LOCUS       DAWFIE010000001        73885 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWFIE010000001 DAWFIE010000000
VERSION     DAWFIE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 73885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73885
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.001"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(1..83)
                     /locus_tag="WGQ71_000001"
                     /pseudo
     CDS             complement(1..83)
                     /locus_tag="WGQ71_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
     gene            complement(188..685)
                     /locus_tag="WGQ71_000002"
     CDS             complement(188..685)
                     /locus_tag="WGQ71_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HEZ0706103.1"
                     /translation="MNVNVHKDTHHFGIHNLNNTDVHLTAEDSDNVIEIKGELADPMP
                     TGNVESTSFLRGIETAFKSNTVLTGNNNKISIATEGKGLGLGVNGLQSNSPYSGHTTG
                     IFINNQSSDLTQPSLDGISTIKLKAVKGNNEVDLNVKNHASVKGIIASHSAKATLEAE
                     GDNIV"
     gene            complement(779..1192)
                     /locus_tag="WGQ71_000003"
     CDS             complement(779..1192)
                     /locus_tag="WGQ71_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706104.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNLQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASEKNTDKQ"
     gene            1504..2814
                     /locus_tag="WGQ71_000004"
     CDS             1504..2814
                     /locus_tag="WGQ71_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ0706105.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYSA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            2928..5933
                     /locus_tag="WGQ71_000005"
     CDS             2928..5933
                     /locus_tag="WGQ71_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ0706106.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNENWDKPTNIFPKI
                     FIESLCRINPDLSPDDARRLLTDIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEEFRPDITLLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     SKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSAYGKPVFNYFREEHKLNLAEL
                     LNTLSDGLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLSFLLQHGL
                     TYVKASQGLVQKHIMRYPQLFATLAIEKHLANGGKKGVIWHTQGSGKTALAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLVVHTIDSREAFAADIKSAQTLHN
                     HAGKAEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREEIGSRYK
                     AQLQEALAQLEIEKGSFDRKEIYAHPHFVHPMLDYILDDFAKFRKTNQDDSLGAMVVC
                     DSAEQARQLFEHFQIASDHNFTAALILHDVGTKDERDQWVKDFKAGKIDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAY
                     WDELSNELGDEIGSYSRLFKTAEEIEQEIADIKNALFDFDTENAEEFCSQISQIADKK
                     QLLALKKALQTSKKLYNVLRLQGSHEFLAHLDFDKLNLLYRETAARLETLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQEDPEYISLR
                     EGLERIFKKKNLAEVGQEEMQANIAILETVYAKIKELNRQNDLLRHKYGGDAKYARIH
                     KRLRENPALYGDKLKVFNALNGVKTDTDQKVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQAINRLLVREYLKESGRI"
     gene            6058..7215
                     /locus_tag="WGQ71_000006"
     CDS             6058..7215
                     /locus_tag="WGQ71_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0706107.1"
                     /translation="MNSALKNYAAYVNNKIDVCEISLNQYISTDNILQNKQGIECAAS
                     LPIQGGKVTAFKKGDILLANIRPYLKKIWYAQFDGGCSADVLAIRANAKIDSHFLFYA
                     LFRDDFFIHAMKGSKGTKMPRGDKTQIMEFKIPVFAPQTQQSITTVLSALDKKIALNK
                     QINARLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGEMVFDETLKREIPKGWESVELQ
                     SCLAKVPSTVKISNKDIKDFGKYPVIDQSQDFICGFTDDEKSILNPQDAHIIFGDHTR
                     IVKLVNFKYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYARHFKFLKEFPII
                     LPDKDISRKYYEIVNYFFIKIRNNIKQNHHLTQLRDFLLPMLMNGQVSVAE"
     gene            7264..8865
                     /locus_tag="WGQ71_000007"
     CDS             7264..8865
                     /locus_tag="WGQ71_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0706108.1"
                     /translation="MTEQHFTEQTKVLIDSLKTICAHYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKKVRKENPNDPIEFVNMDIEGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAACNAELFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEDVRGQIRSVD
                     VYDPSAGSGTLLMNVAHAIGEDKCMIYTQDISQKSSNLLRLNLILNNLVHSLNNVVQG
                     NTILSPAHKDASGCLKKFDFIVSNPPFKLDFSDFRDRLESDENHERFFAGIPKIKPTK
                     KEKMEIYQLFIQHILFSLKENGKVAIVLPTGFITAKSGIDKKIREYLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKVNKDKVVLIDASGLGEKISINDNQKTVLSHEEEQKICH
                     TFTHKQAVEDFSVVVGYDEIKAKNYSLSAGQYFEVKIDYVDISAEEFEQKMAEFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            8876..9013
                     /locus_tag="WGQ71_000008"
     CDS             8876..9013
                     /locus_tag="WGQ71_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0706109.1"
                     /translation="MKPVKLKMPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYR
                     K"
     gene            complement(9173..10510)
                     /gene="ribB"
                     /locus_tag="WGQ71_000009"
     CDS             complement(9173..10510)
                     /gene="ribB"
                     /locus_tag="WGQ71_000009"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ0706110.1"
                     /translation="MTDTADLRRRNLRQWIEKHYGGLQTRFAEAVCLNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPSAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHNLKIGTIT
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPTADEETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRAEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLIGFGLEVVGFEEAEK"
     gene            complement(10725..11739)
                     /locus_tag="WGQ71_000010"
                     /pseudo
     CDS             complement(10725..11739)
                     /locus_tag="WGQ71_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(11907..12500)
                     /gene="ribA"
                     /locus_tag="WGQ71_000011"
     CDS             complement(11907..12500)
                     /gene="ribA"
                     /locus_tag="WGQ71_000011"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ0706111.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(12493..13170)
                     /locus_tag="WGQ71_000012"
     CDS             complement(12493..13170)
                     /locus_tag="WGQ71_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706112.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAEYLTAQTVMDMGTASIERSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNIASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            13324..13408
                     /locus_tag="WGQ71_000013"
     tRNA            13324..13408
                     /locus_tag="WGQ71_000013"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13358..13360,aa:Leu,seq:tag)
     gene            13928..14962
                     /gene="purM"
                     /locus_tag="WGQ71_000014"
     CDS             13928..14962
                     /gene="purM"
                     /locus_tag="WGQ71_000014"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ0706113.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
     gene            complement(15436..16092)
                     /locus_tag="WGQ71_000015"
     CDS             complement(15436..16092)
                     /locus_tag="WGQ71_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0706114.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHDFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
     gene            complement(16089..17861)
                     /locus_tag="WGQ71_000016"
     CDS             complement(16089..17861)
                     /locus_tag="WGQ71_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ0706115.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGTTVETVWENGTHLPTQDEQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAV
                     LKIYSQPQQGTTVSLAGVSEESLK"
     gene            18067..18681
                     /locus_tag="WGQ71_000017"
     CDS             18067..18681
                     /locus_tag="WGQ71_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ0706116.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPGDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQIDLQEG"
     gene            18844..19461
                     /locus_tag="WGQ71_000018"
     CDS             18844..19461
                     /locus_tag="WGQ71_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ0706117.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            19469..20332
                     /locus_tag="WGQ71_000019"
     CDS             19469..20332
                     /locus_tag="WGQ71_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0706118.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(20345..20779)
                     /locus_tag="WGQ71_000020"
     CDS             complement(20345..20779)
                     /locus_tag="WGQ71_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ0706119.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            20925..21653
                     /gene="rpe"
                     /locus_tag="WGQ71_000021"
     CDS             20925..21653
                     /gene="rpe"
                     /locus_tag="WGQ71_000021"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ0706120.1"
                     /translation="MTAYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(21676..22707)
                     /gene="ruvB"
                     /locus_tag="WGQ71_000022"
     CDS             complement(21676..22707)
                     /gene="ruvB"
                     /locus_tag="WGQ71_000022"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ0706121.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(22789..23097)
                     /locus_tag="WGQ71_000023"
     CDS             complement(22789..23097)
                     /locus_tag="WGQ71_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706122.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            complement(23320..24573)
                     /locus_tag="WGQ71_000024"
     CDS             complement(23320..24573)
                     /locus_tag="WGQ71_000024"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ0706123.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWFALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKTFRAAQDKTEKH"
     gene            complement(24645..26333)
                     /locus_tag="WGQ71_000025"
     CDS             complement(24645..26333)
                     /locus_tag="WGQ71_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ0706124.1"
                     /translation="MLQSENSKNLISCSIKALPIMISTNGITMQFGAKPLFENVSVKF
                     GEGNRYGLIGANGSGKSTFMKILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEEMRVL
                     DVVMMGHTEMWAAMTERDAIYANLEATEDDYMKAAELEAKFAEYDGYTAEARAAELLS
                     GVGISEDLHNATMAEVAPGFKLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVL
                     NQYDSTMIIISHDRHFLNEVCTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNA
                     KAKEKLQELQEFVARFSANKSKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEAD
                     EKAKLHRQAVEVEKLAKRFETQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAY
                     NPEYSDGLLPDEGTIKWAEKASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIR
                     GTLGRLLFGSNDVVKKVKVLSGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESL
                     NMALEKYNGTLIFVSHDRQFVSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            26423..26797
                     /locus_tag="WGQ71_000026"
     CDS             26423..26797
                     /locus_tag="WGQ71_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ0706125.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAEYGMEFEFRDFKKQVPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQALCRQG"
     gene            26852..28390
                     /locus_tag="WGQ71_000027"
     CDS             26852..28390
                     /locus_tag="WGQ71_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ0706126.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAMQNEQADGGSPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            28457..29074
                     /gene="recR"
                     /locus_tag="WGQ71_000028"
     CDS             28457..29074
                     /gene="recR"
                     /locus_tag="WGQ71_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ0706127.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(29130..29408)
                     /locus_tag="WGQ71_000029"
     CDS             complement(29130..29408)
                     /locus_tag="WGQ71_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706128.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSDSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            29613..30860
                     /locus_tag="WGQ71_000030"
     CDS             29613..30860
                     /locus_tag="WGQ71_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ0706129.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDAVWVRDWTYNNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            30853..31548
                     /gene="lolD"
                     /locus_tag="WGQ71_000031"
     CDS             30853..31548
                     /gene="lolD"
                     /locus_tag="WGQ71_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ0706130.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAETAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFGRVMVM
                     KDGNLHPKQGANA"
     gene            31616..33361
                     /gene="recD"
                     /locus_tag="WGQ71_000032"
     CDS             31616..33361
                     /gene="recD"
                     /locus_tag="WGQ71_000032"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ0706131.1"
                     /translation="MERQTDEFAQAAARAAIWFLARYAGSSDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFDHIRPLPLDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(33433..34536)
                     /locus_tag="WGQ71_000033"
     CDS             complement(33433..34536)
                     /locus_tag="WGQ71_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0706132.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPVHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(34511..34702)
                     /locus_tag="WGQ71_000034"
     CDS             complement(34511..34702)
                     /locus_tag="WGQ71_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HEZ0706133.1"
                     /translation="MEFDAMPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVL
                     PPYFQNMTQVFHVFFYFSR"
     gene            34787..37249
                     /gene="lon"
                     /locus_tag="WGQ71_000035"
     CDS             34787..37249
                     /gene="lon"
                     /locus_tag="WGQ71_000035"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ0706134.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAALEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEISEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAAAESASKPKPRSRATKH"
     gene            37431..37700
                     /locus_tag="WGQ71_000036"
     CDS             37431..37700
                     /locus_tag="WGQ71_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ0706135.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(37759..38193)
                     /locus_tag="WGQ71_000037"
     CDS             complement(37759..38193)
                     /locus_tag="WGQ71_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706136.1"
                     /translation="MTEPKHEMLTKEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(38186..39493)
                     /locus_tag="WGQ71_000038"
     CDS             complement(38186..39493)
                     /locus_tag="WGQ71_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ0706137.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAAEISRRLGREVRISAVCD
                     LSEEKARQICPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFSLAEEKNVMVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            39666..40061
                     /locus_tag="WGQ71_000039"
     CDS             39666..40061
                     /locus_tag="WGQ71_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706138.1"
                     /translation="MMRRRGKKMLFEENPIDEHFAEYDRRTGEIRLSGRHFNKPVLLH
                     RDSAGLSKWQSLSELTPDNLLSDVKADDYPEILIIGTGAAQEFIHPKIMADFSRIGIG
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            40138..42060
                     /locus_tag="WGQ71_000040"
     CDS             40138..42060
                     /locus_tag="WGQ71_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0706139.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFAGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSLLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            42526..43041
                     /locus_tag="WGQ71_000041"
     CDS             42526..43041
                     /locus_tag="WGQ71_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706140.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(43331..43465)
                     /locus_tag="WGQ71_000042"
     CDS             complement(43331..43465)
                     /locus_tag="WGQ71_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ0706141.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT
                     "
     gene            complement(43607..44575)
                     /locus_tag="WGQ71_000043"
     CDS             complement(43607..44575)
                     /locus_tag="WGQ71_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0706142.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAVIQPGSRPGSRPGRLIR
                     KWSESLLDAFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKES
                     DEAANLTVVFEETETGKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSE
                     LERLSQELENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQ
                     TLMTQEKAAKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLE
                     NSIQQLISRLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            complement(44645..44761)
                     /locus_tag="WGQ71_000044"
                     /pseudo
     CDS             complement(44645..44761)
                     /locus_tag="WGQ71_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(44893..45369)
                     /locus_tag="WGQ71_000045"
     CDS             complement(44893..45369)
                     /locus_tag="WGQ71_000045"
                     /inference="COORDINATES: protein motif:HMM:NF014442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ0706143.1"
                     /translation="MLHLLKSLKPSGKGAIILPHGVLFRGNAEARIRTELLNLDLIKG
                     IIGLPANLFYGTGIPACIIVIDKEHAQTAQFAEEGTNQVISGGSVFMIDASRGFIKDG
                     NKNRLREQDIHKIIDTFTKQEEIPRYSRMVHLSEIAAQDYNLNLPRYIDSGEVEDL"
     gene            complement(45257..45613)
                     /locus_tag="WGQ71_000046"
     CDS             complement(45257..45613)
                     /locus_tag="WGQ71_000046"
                     /inference="COORDINATES: protein motif:HMM:NF014442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0706144.1"
                     /translation="MLKAAAQAGSQISLYGQEKDVATASLARMNMILHNNETAEINTG
                     NTLSDSSFRDENDGLKTFDFAVANPPYPARKKRRLRLFAASAQKPETKRQRCDYSSAR
                     CAVSRQCRSAYSHGIA"
     gene            complement(45526..46173)
                     /locus_tag="WGQ71_000047"
     CDS             complement(45526..46173)
                     /locus_tag="WGQ71_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0706145.1"
                     /translation="MHHFGRAATSCAAVWMRAFIKNYVLTLLFLKYVSDKHKYGGGMI
                     ELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVINVTDFNDEDKLGKGKEM
                     IDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRI
                     MAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            complement(46070..46279)
                     /locus_tag="WGQ71_000048"
     CDS             complement(46070..46279)
                     /locus_tag="WGQ71_000048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0706146.1"
                     /translation="MNADIAVAAGVLKSRLICNFHLLGKTLWQSKKNQLYASLWAGCD
                     ELRGGMDASLYKELCAYAIVFKICF"
     gene            complement(46276..46935)
                     /gene="ung"
                     /locus_tag="WGQ71_000049"
     CDS             complement(46276..46935)
                     /gene="ung"
                     /locus_tag="WGQ71_000049"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ0706147.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLAAHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            47016..47423
                     /locus_tag="WGQ71_000050"
     CDS             47016..47423
                     /locus_tag="WGQ71_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ0706148.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYAEILRHVGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIITHP"
     gene            47438..48385
                     /locus_tag="WGQ71_000051"
     CDS             47438..48385
                     /locus_tag="WGQ71_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ0706149.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(48581..49651)
                     /locus_tag="WGQ71_000052"
     CDS             complement(48581..49651)
                     /locus_tag="WGQ71_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ0706150.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYMEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            49836..50111
                     /locus_tag="WGQ71_000053"
     CDS             49836..50111
                     /locus_tag="WGQ71_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ0706151.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            50114..50737
                     /gene="lipB"
                     /locus_tag="WGQ71_000054"
     CDS             50114..50737
                     /gene="lipB"
                     /locus_tag="WGQ71_000054"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ0706152.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            50730..51698
                     /gene="lipA"
                     /locus_tag="WGQ71_000055"
     CDS             50730..51698
                     /gene="lipA"
                     /locus_tag="WGQ71_000055"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ0706153.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRECEF"
     gene            51761..53504
                     /locus_tag="WGQ71_000056"
                     /pseudo
     CDS             51761..53504
                     /locus_tag="WGQ71_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628995.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            53776..54900
                     /locus_tag="WGQ71_000057"
     CDS             53776..54900
                     /locus_tag="WGQ71_000057"
                     /inference="COORDINATES: protein motif:HMM:NF015210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEZ0706154.1"
                     /translation="MKNRDTPHKKFINPTEYSLFAEEEQYDFELMIENQIKEHEKEID
                     YYVTDYPIETLVAKLDRQELFIPDYQRKNIWEKHRKSRFIESVLIGLPIPFLFFWQDP
                     STGNMEVVDGAQRLTALSEFIHGNLKLSYLEKLIYLNGQTYHQLLESRQKIFKNKPIR
                     GVVLSNKTDKEARLDLFERLNTGSKNATPAEIRRGLLQGAFYDMVETLSEDKTFNELA
                     KISKNMQDMGEREEFITRFFAYGEYLDHYSANVRKFIFDYTEIMNEEFAKNPNMRATY
                     EQKFHEIMAFFKEKYPIGFKRDGKGQSSRTRFEGMAVSAYLAEKENPGILAQVTPKEC
                     VDIFDSKEFIEVTGSDGANATKKLHARLFFAKNAFKRNHP"
     gene            54897..55649
                     /locus_tag="WGQ71_000058"
     CDS             54897..55649
                     /locus_tag="WGQ71_000058"
                     /inference="COORDINATES: protein motif:HMM:NF037890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706155.1"
                     /translation="MNSVKSMMESREEEINLYMDLLKYIHDSTQHGLPALQNRNGVEY
                     QLENTHLHILTASIYMLLYNYIEAVVTQSLNTLICYIQNERMPITQFSEPIQKEWLIG
                     GLNLNDAKASVETRLGNGLKMLKHFGDTQKIDIKISKGGGNFNDDEIFKLAKKLQVNM
                     CIDDETHRQLRQAGNITYESTRKFQDTCMKEITRERNKLAHGEITFQECGRNRTISDL
                     MAIKDTTFRYMHLFTDAFTEYLMQKKYLKPTV"
     gene            complement(55633..56655)
                     /locus_tag="WGQ71_000059"
     CDS             complement(55633..56655)
                     /locus_tag="WGQ71_000059"
                     /inference="COORDINATES: protein motif:HMM:NF012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0706156.1"
                     /translation="MKIEAVDLFCGAGGLTAGLLRAGIKVKAGYDIDAACAYAYEYNN
                     KGAKFILQDVETVTGDQIQAHYSKDAVRLLAGCAPCQPFSTYNQGRDVRNDKKWPLLY
                     AFSRLIHEVQPELVTMENVPDVVKHEVYQDFVNNLKQQGYQVWAEKVLCADYGVPQSR
                     RRHVLLASRIGNIQMVPPTHSNCHVTVRQAIGHLPPLAAGKQDTIDVLHRCSSMSARN
                     LERIRASRPGGTWMDWPEDLRAACHRKKKGQSYKRIYGRMEWDKPAPTMTTLCIGFGN
                     GRFGHPEQDRAISLREAALFQSFPLDYQFTQETDTPIRQISRMIGNAVPVLLGEAIGN
                     SFKLLV"
     gene            complement(57089..57961)
                     /locus_tag="WGQ71_000060"
     CDS             complement(57089..57961)
                     /locus_tag="WGQ71_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HEZ0706157.1"
                     /translation="MNYPHLSPKAKEQLKYSDIERIEYIRSPRWIGYPQAQQVLVKLE
                     DLLSFPKQNRMPNMLLVGDTNNGKTMLIKHFLRQHPAHDNPHGNGIIAPVVIIQAPPI
                     PDESRFYNAILDLLFAPYKTHDRTDKKLAQVLHLLKYVNTKMLIIDEIHHILSGSLNK
                     QKTFLNVIKYLGNELQIPNPDELFTSWLVRLAHANGLKVQTFCHLLFPNYEIWNRDID
                     RHTPDWLVNTLAEKTGCSTIRIKTTTLDSLQGRLFDKNNFSGQLTWINSLKANHRKRD
                     NNAIVYCPLCLATD"
     gene            complement(57958..59283)
                     /locus_tag="WGQ71_000061"
                     /pseudo
     CDS             complement(57958..59283)
                     /locus_tag="WGQ71_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687573.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(59296..59565)
                     /locus_tag="WGQ71_000062"
                     /pseudo
     CDS             complement(59296..59565)
                     /locus_tag="WGQ71_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(59568..60107)
                     /locus_tag="WGQ71_000063"
                     /pseudo
     CDS             complement(59568..60107)
                     /locus_tag="WGQ71_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714285.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(60104..60310)
                     /locus_tag="WGQ71_000064"
                     /pseudo
     CDS             complement(60104..60310)
                     /locus_tag="WGQ71_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease C-terminal domain-containing
                     protein"
     gene            complement(60324..60743)
                     /locus_tag="WGQ71_000065"
     CDS             complement(60324..60743)
                     /locus_tag="WGQ71_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HEZ0706158.1"
                     /translation="MYAFYCHINKMAYLANTAKHIVGVGIKDAYRAIQTDAQLANRFE
                     PAVLPRWELNKDFMRLLMSFERMLPLSQPSDLHEISLATKLYGMSEGYIGELSRLLTQ
                     AAVAAIQQQTECINETILNSINWISPSQRKRQLDKLR"
     gene            complement(60664..61134)
                     /locus_tag="WGQ71_000066"
     CDS             complement(60664..61134)
                     /locus_tag="WGQ71_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7 transposase TnsA N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0706159.1"
                     /translation="MPIRKIPKNYRNITGIAPYNKAIGIAAYESSLERDFLTLLEFDS
                     NVQHFEVQPITIEWFDPAGKKHIYTPDVLVHYQNDVIIYEVKYRSDLRKNWCELKPKF
                     QAALHFCKLHGWHFKLITELEIHTDYLCNVRFLLPYQQNGLSGEYSEAYCRCRY"
     gene            61346..61504
                     /locus_tag="WGQ71_000067"
     CDS             61346..61504
                     /locus_tag="WGQ71_000067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706160.1"
                     /translation="MEFTAKATAVAGIIKPGTPRQSEERFPAPAAPFDVNNINLNCPC
                     RLKALQTA"
     gene            61858..62322
                     /gene="bfr"
                     /locus_tag="WGQ71_000068"
     CDS             61858..62322
                     /gene="bfr"
                     /locus_tag="WGQ71_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0706161.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            62350..62823
                     /gene="bfr"
                     /locus_tag="WGQ71_000069"
     CDS             62350..62823
                     /gene="bfr"
                     /locus_tag="WGQ71_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0706162.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALI
                     AAISVAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            63147..63467
                     /locus_tag="WGQ71_000070"
     CDS             63147..63467
                     /locus_tag="WGQ71_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0706163.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ERGI"
     gene            63515..66073
                     /gene="glnD"
                     /locus_tag="WGQ71_000071"
     CDS             63515..66073
                     /gene="glnD"
                     /locus_tag="WGQ71_000071"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ0706164.1"
                     /translation="MPANLSSALETFKRQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWVEHFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGGIGELCESVRDDITGDTAFLEARFLFGNRQTADELAKKMNAQRNVPA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATNLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNANRAEDRLLFDLQPQVAESMGYQGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLRSRVSSAPMHVTLRIDDDYIQVNNQIAARHT
                     DIFLRQPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNPENRRRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIIGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETSSYSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSLDLKNPKIQSSLKQALLEQLT"
     gene            66170..66631
                     /locus_tag="WGQ71_000072"
                     /pseudo
     CDS             66170..66631
                     /locus_tag="WGQ71_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            66677..66757
                     /locus_tag="WGQ71_000073"
                     /pseudo
     CDS             66677..66757
                     /locus_tag="WGQ71_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            66958..68421
                     /gene="guaB"
                     /locus_tag="WGQ71_000074"
     CDS             66958..68421
                     /gene="guaB"
                     /locus_tag="WGQ71_000074"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ0706165.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVRWVKKTYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            68568..68765
                     /locus_tag="WGQ71_000075"
     CDS             68568..68765
                     /locus_tag="WGQ71_000075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706166.1"
                     /translation="MPSERRLRPVCRIPPAFKTAVEINAIFQYGRFDWPEMMRRPPTS
                     KNRVEASGKYAPPPQAAPSAL"
     gene            complement(68760..71135)
                     /gene="rnr"
                     /locus_tag="WGQ71_000076"
     CDS             complement(68760..71135)
                     /gene="rnr"
                     /locus_tag="WGQ71_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ0706167.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPVHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIADVSHYVRPDDVIDADAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPDVERLCMVCDMVVTYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWISDGIDHPYKAQIDTLYKLFKILQKKRFERGAVEFESVETQMIFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESRKKAKKPVPIKVKKRKGKS"
     gene            71224..71310
                     /locus_tag="WGQ71_000077"
     tRNA            71224..71310
                     /locus_tag="WGQ71_000077"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71258..71260,aa:Leu,seq:cag)
     gene            71346..71432
                     /locus_tag="WGQ71_000078"
     tRNA            71346..71432
                     /locus_tag="WGQ71_000078"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71380..71382,aa:Leu,seq:cag)
     gene            71471..71561
                     /locus_tag="WGQ71_000079"
     tRNA            71471..71561
                     /locus_tag="WGQ71_000079"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71505..71507,aa:Ser,seq:gga)
     gene            complement(71971..73782)
                     /gene="typA"
                     /locus_tag="WGQ71_000080"
     CDS             complement(71971..73782)
                     /gene="typA"
                     /locus_tag="WGQ71_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ0706168.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT        19163 a        18287 c        18259 g        18176 t
ORIGIN      
        1 tgacgcaaat gccgttttgt acaattccaa aagttttcaa ttccgttaat gcgattttgc
       61 cgtttggcga aatgcgtact gttccagtcg tggattgaac ccccaccctg tatagttctt
      121 tcgaagcatt ggggtattgt tttttcaaag catcgattaa agccgtattg gtgtccggat
      181 ttttaaccta gacaatattg tccccttcgg cttcaagcgt tgcttttgca gaatgcgacg
      241 cgataatgcc ttttacggag gcgtggtttt ttacatttaa atcaacttca ttatttccct
      301 tgacggcttt gagtttgatg gtgctgatgc catcaaggga aggttgggta aggtcgcttg
      361 attgattatt gatgaaaatg cccgtcgtgt gtccggaata gggggagttg gactggagac
      421 catttacgcc caatcccaag cccttgccct cagtggcgat ggatatttta ttgttattcc
      481 ccgttaatac ggtattggat ttaaaagccg tttcaatgcc ccttaaaaaa cttgtactct
      541 caacatttcc tgtcggcata gggtcggcga gctccccttt aatctcaatc acattatcgc
      601 tgtcttcggc agtcagatga acatcggtat tgttgaggtt gtgaataccg aaatggtgtg
      661 tgtctttatg aacgttaacg ttcatattga acaaattgcc tgtttttgaa tgaaaggata
      721 tgctgccttt gtcattttca tcggggggct gatagtgttg acataccgaa ctaaggcact
      781 attgtttgtc ggtatttttt tcactggcta catcttgtac cccatcaaca tcgggaacat
      841 tcaaattaaa ttgaattttt cctttggcat ccaaatggat atttccggaa tggggcttca
      901 cgtcaacaga ttggagatta tttttaaata gaatatcatt attgcttttt accaataatt
      961 caggcgtata tcccaattga gagtctgcaa aagattggat gccgtaaatt ccatttaccg
     1021 ttaaatttcc attatcattt ttaccagtgg taataagttc taattttcta aagttttcac
     1081 cttttttcca ccaaggcaca atatagtttt ttacagtgat tttttcatct ttaagggtta
     1141 taagactatc atcgtctctt tgcggatcag aaaataaaga cacccattcc atctcttgaa
     1201 tattagtata ttcatgggct tccgcctcaa aacataatgc cgattgaatc aacaaagcaa
     1261 tggtagataa agcaaatttt ctattttagt ctccttaatg tttaattgaa acgctgattt
     1321 gggtattgtc atgccgatgc ggaatcggtc tccgtgccgg atatattgtg tgaagtctct
     1381 cgtcttttcc agtatgcgcc taatatcggc ttaaggcaaa cggaggggcg gaatccgtat
     1441 gcggcacgcc gccgttccct gccgtataat catattgttt gaaaacctgt tgacggagcc
     1501 gttatgaccg atttgtttgc ccgagaaccc gacgcgccgc ttgccgaacg attgcgtccg
     1561 catacgcttg acgacgtggt ggggcaggaa cacctcatcg gcgaaggtaa acctttgcgc
     1621 gtggcggtag aaggcggcaa gccgcattct atgttgctgt gggggccgcc gggcgtgggc
     1681 aagacgacgt tggcgcggat tttggcgcag agtttcaacg cccagttttt gcctgtttcc
     1741 gccgtatttt ccggcgtgaa ggacatccgc gaggcaatcg ataaagccga aatcgctttg
     1801 cagcagggac gcgcgacgat tttgtttgtc gatgaagtcc accgcttcaa caaggcgcag
     1861 caggacgcgt ttttgccgca tgtcgaaagc ggtttgctaa cctttattgg tgcgacgacg
     1921 gaaaatccgt cgtttgaagt caatcccgcg ctgttgagcc gcgctcaggt gtatgttttg
     1981 caacccttgt cttcagacga cctgaaaaag ctgattgcca aggtattggc tttgcctgaa
     2041 taccaagagt ttacgattga aacggatgcg caaaaattac tcgtgaatac cgccgacggt
     2101 gatgcgcgca gattgttgaa tttgttggaa caacttttac gcgccgccga tacacgtcgt
     2161 ctgaaaaact taaccgccga atttctcgcc gacagtctcg gggcgcaaat ccgccgtttc
     2221 gacaaaggcg gcgagagttt ctacaaccaa atctccgccc tgcacaaatc cgtgcgcggt
     2281 tcgcatccga acgccgcgct gtattggttc tgccgtatgc tcgacggcgg caccgacccg
     2341 cgctacctcg cccgccgcat cgtgcgtatc gcttgggaag acatcgggct tgccgacccg
     2401 cgcgccttcc aaatcgccaa cgatgccgcc gccaccttcg aacgcttagg ctcgcccgaa
     2461 ggcgaactgg ctttggcgca agcggtattg tatcttgccg ccgccgcgaa atccaacgcg
     2521 ggctacaagg catacaacca aatgcgccac ttcgtcaaag aaaacgctag cgacgaagtg
     2581 cccgtccacc tgcgcaacgc cccgaccaag ctgatgaagg aattgggcta cggacgcgaa
     2641 taccgctacg cccacgacga accgaacgcc tactccgccg gcgaaagcta tatgcccgac
     2701 ggcttggacg aaccggactt ctaccaaccc gtcccgcgcg ggttggaaat caaaatcggc
     2761 gaaaagctgg cgtggctcaa atcgctggat gaagaggcat taaaggcaaa atgaagcaat
     2821 gccgtctgaa gcagaacagt atggcaaagc cggtacaccg aaaaggcgta tcgggttttt
     2881 caggtgttat aatccgtttc aataaatttt gatgaatcat tacgtttatg tcccaaactc
     2941 acaacgaaaa ttcccgcgtc aaaatccccg ccgttttgca cctgatgcgc ttgggctacg
     3001 actacctctc gctcaaaaac gaaaattggg ataaaccaac caacatcttt cccaaaatct
     3061 ttatagaaag cctctgccgc atcaatcccg atttgtcgcc ggacgatgca cgccgtttgc
     3121 tgaccgacat acgcttagaa ctggacaacg aagacttggg gcaaaagttt tacgaacgcc
     3181 ttaccaatca atccggcggc aagaaactga tcgacttcca aaattttgat aacaacagct
     3241 tccacgttgt aaccgaattg ccctgtatca acggcgatga agaattccgc cccgacatta
     3301 ccctgctggt aaacggcatg cctttggttt ttatcgaagt caaaaagccc aacaacaaag
     3361 gcggaatcgg cgaagagcgg gagcgcatgg gcaaacgtgc gaaaaattcc aaatttcgcc
     3421 gtttcatcaa catcacccaa ttcatgattt tttccaacaa catggaatac gacgacggcg
     3481 caaccgaacc ggcacaaggc gcgttttacg cttcgtctgc ttacggtaag cccgtgttta
     3541 actacttccg cgaagaacat aaattaaatc ttgccgaatt attgaacacg ctttcagacg
     3601 gcctcgaaaa caacgtcctg caagacaaca acctgcccgt tatcaaacac agccccgaat
     3661 ttatcagcaa taaatcgccc gatacgccga ccaaccgcat cctgacttcg ctgctttgcc
     3721 gcgaacgtct ttcttttctt ctgcaacacg ggctgactta cgtcaaagca agtcaaggct
     3781 tggtgcaaaa acacatcatg cggtatccgc agctatttgc cactctcgcc attgaaaaac
     3841 atttggcaaa cggcggcaaa aaaggcgtga tttggcacac ccaaggctct ggcaaaaccg
     3901 cgcttgccta ttacaacacc cgctacctga cccactatta tgccaaacag ggcatcgtgc
     3961 cgaaattcta tttcatcgtg gacaggcttg atttattgaa acaggcgcag cgcgaattta
     4021 ctgcccgcga tttagtcgtc cataccatcg acagccgcga agcctttgct gccgacatca
     4081 aatccgccca aactttgcac aaccacgcag gcaaggcgga aatcactgtc gtcaacatcc
     4141 aaaaattcca agacgacccc gatgtcgtcg cccgtaacga ctacgacctc gccatccagc
     4201 gcgtttattt tctcgacgaa gtacaccgca gctacaaccc caaaggctcg tttcttgcca
     4261 acctcaacca gtccgacgta aacgccgtca aaatcgggct gaccggcacg ccgcttatcg
     4321 gcgtaaccgc aggcaacgtc aacacccgcg aactcttcgg cgactacatc cacaaatact
     4381 attacaacgc ctccattgcc gacggctaca ccctgcgcct gatacgcgaa gaaatcggca
     4441 gccgatacaa ggcgcaatta caagaagcac tggcgcaact tgaaatcgaa aaaggcagct
     4501 ttgaccgcaa agaaatctac gcccatccgc acttcgtcca cccgatgctt gactacatct
     4561 tggacgactt cgccaaattc agaaaaacca accaagacga cagcctcggc gctatggttg
     4621 tttgcgacag cgcggaacag gcacgtcagc tttttgagca ttttcagata gcctcagacc
     4681 ataatttcac cgccgcgctg atattgcacg acgtcggcac taaggacgaa cgcgaccaat
     4741 gggttaaaga tttcaaagcg ggcaaaatcg acatcttgtt tgtgtacaac atgcttttga
     4801 ccggctttga cgcgccgcgc ctgaaaaagc tctatttggg cagactgata aaagcccata
     4861 accttttaca aaccctgacc cgcgtcaacc gcacctacaa atcttaccgc tacggctatg
     4921 ttgtcgattt tgccgacatc gagcgcgaat ttgacaaaac caaccgcgcc tattgggacg
     4981 aactttccaa cgaattgggc gacgaaatcg gcagctacag ccggcttttc aaaaccgccg
     5041 aagaaatcga acaggaaatt gcagacatta aaaacgccct gtttgatttc gataccgaaa
     5101 acgccgaaga attttgcagc caaattagcc aaatagcaga caaaaaacag ctgctcgccc
     5161 taaaaaaagc cctgcaaact tccaaaaagc tgtacaacgt cctgcgcctg caaggcagcc
     5221 acgaatttct cgcccatctc gattttgaca agctcaatct tttgtaccgc gaaaccgccg
     5281 cgcgtttgga gaccttaaac cttgccgaaa aactacagca aggcgacacc gcccatttgc
     5341 tcaatgaagc gctggaagac gtttactttc aattcgttaa aatcggcgaa gccgaactca
     5401 aacttgccga cgatttgaaa gacatcatgc gcaaagtccg cgaagggctg gcgggcaact
     5461 tcgatcaaga agaccccgaa tacatcagcc tgcgcgaagg actggaacgc atcttcaaga
     5521 aaaagaacct cgccgaagtc gggcaagaag aaatgcaggc aaacatcgcc atactcgaaa
     5581 ccgtctatgc caaaatcaaa gagctgaacc gccaaaacga cctcttgcgg cacaaatacg
     5641 gcggcgacgc caaatatgcc cgcatccaca aacgcctgcg cgaaaacccc gccctctacg
     5701 gcgataaact caaagtcttt aacgcgctga atggtgtaaa aaccgacacg gaccaaaaag
     5761 tgctggatat ggagcagatt ttggacaacc aaaattactt tgaaaaacaa atgcagggca
     5821 tcgtattaaa acgctttaga acggaacagc aatttcctgt tcagcctgca gacatccaag
     5881 ccatcaaccg cttgctggta cgggaatatt taaaagaaag cgggcggatt tgaggaattg
     5941 gtttcaggca gcctttacgg gcgtattgca tacgttcgaa gtggacaata aaagtgcggt
     6001 cggattttga aatgtttttc agacggcctt tgaagagttt tgagagataa aatcaatatg
     6061 aatagtgcac taaagaatta tgcagcatat gtaaataata aaattgatgt atgtgaaatt
     6121 tcgcttaacc aatatatttc aacagataat atcttgcaaa ataagcaagg aatagagtgt
     6181 gcagcatctt taccgattca aggtggaaaa gtaactgcat ttaaaaaggg tgatatttta
     6241 ttagctaata tacgaccgta tctaaaaaaa atttggtatg cccaatttga tggaggttgt
     6301 tctgctgatg ttttggcgat tcgtgcaaac gccaaaatag atagtcattt cttattttat
     6361 gctttgtttc gtgatgattt ttttatccat gcaatgaaag gttcaaaggg cacaaagatg
     6421 ccgcgaggcg ataagacaca gattatggag tttaaaatcc ctgtttttgc ccctcaaacc
     6481 cagcaatcca tcaccaccgt cctatccgct ttggacaaaa aaatcgccct gaacaaacaa
     6541 atcaacgcgc gcttggaaga gatggcgaaa accctgtacg actactggtt tgtacagttc
     6601 gattttcccg acgccaatgg caagccctac aaatcctcag gcggcgaaat ggtgtttgac
     6661 gaaaccttga aacgggaaat tccgaagggg tgggaaagcg tagaattgca atcttgctta
     6721 gcaaaagtcc caagtactgt aaaaatatca aataaagaca ttaaggattt tggaaaatat
     6781 cctgttattg accaaagtca agattttatt tgcgggttta ctgacgatga aaaatctata
     6841 ttaaaccctc aagatgctca tattattttt ggtgatcata caagaatcgt taagttagtt
     6901 aattttaaat atgcgcgagg ggcggatggt acacaagtaa tattaagcaa taatgagcga
     6961 atgccaaact atttgtttta ccaaatcata aatcaaatag atttatcaag ttatggatat
     7021 gctagacatt ttaaattctt aaaagagttt ccaattattt tgccagataa agatatttct
     7081 cgaaaatatt atgagatagt aaattatttc tttattaaaa ttcggaataa tataaaacaa
     7141 aaccaccacc taacccaact acgcgatttc ctactgccca tgctgatgaa cgggcaggta
     7201 tccgtggcgg aataagccgg tttcaagaat ccgagcgata caagaaaaag aatgagataa
     7261 aaaatgaccg aacaacactt taccgaacaa accaaagtcc tgattgacag cttaaaaacc
     7321 atttgcgccc actacggttt gggtaatgac ggcaacgaat ttaaaatcat cagtcaggcg
     7381 tttttgtata aattcttaaa cgacaaatac gatttcgaag tcaaaaaagt tcgcaaagaa
     7441 aaccccaatg acccgattga gttcgtcaat atggatattg agggaaaaac agcggttttg
     7501 aagcccgagc attccatcaa atatttgtcg gagcggcaaa acggcgcgga ctttgccaaa
     7561 ctctttgacg atacgctcac tgacatcgcc gcttgtaatg cggagttgtt ttccgtaaag
     7621 acagaaggcg gagcgaaaat cgtattgttc gagcgtatca gtcaatatat tacggatgaa
     7681 ggccgccgcg atgatttttg ccgcgcgctg atttccaaac tggcaggttt tagcttcgaa
     7741 gcgatttttg cgcaaaaatt tgattttttt gccacgattt ttgaatacct gattaaagac
     7801 tacaacagca attcgggcgg taaatacgcc gaatactaca cgccgcatgc agtggcgcgg
     7861 attatggcgg atattttggt gccggaagat gtgcgcgggc aaatccgcag cgtggacgtt
     7921 tacgacccgt cggccggttc gggaacgctg ttgatgaacg tcgcccatgc cataggcgaa
     7981 gacaaatgta tgatttatac gcaggatatt tcgcagaaat cgtccaatct gctgcggctg
     8041 aatctgattt tgaataattt ggtgcattct ttgaataatg tggttcaggg caatacgatt
     8101 ttgtcgcccg cccacaaaga cgcttcaggc tgtctgaaaa aatttgattt cattgtttcc
     8161 aatccgccgt ttaaattgga tttcagcgat ttccgcgatc ggctggaaag cgatgaaaac
     8221 cacgaacgct tttttgctgg tatcccgaaa atcaagccaa caaagaaaga gaagatggaa
     8281 atctatcagc tttttatcca gcatattctg ttcagcctga aagaaaacgg caaggtagcg
     8341 attgtattac cgaccggctt tatcacggca aaatcgggca tcgataaaaa aattcgcgaa
     8401 tatctggtgg aaaacaaaat gctcgccggc gtggtgtcta tgccttccaa tattttcgct
     8461 acgacgggaa ccaatgtttc tatcttgttt attgataaag tcaataagga taaagttgta
     8521 ttaatcgatg cttccggttt aggtgaaaaa attagtatta atgacaacca aaaaactgta
     8581 ctttcccacg aagaagaaca aaaaatctgc cataccttca cgcacaaaca ggcagtagaa
     8641 gatttcagcg tggtggtcgg ctatgatgaa atcaaagcga agaattacag cttgtcggca
     8701 ggtcaatatt ttgaagtaaa aattgattat gtggatattt ccgccgagga attcgagcag
     8761 aaaatggcag aattttcggc agatttggac aagctctttg cggaatctgc cgaattggaa
     8821 aaagagatta aggatagatt ggcgatgttg aaattcaatt cataaatacc gatgaatgaa
     8881 accggtcaaa cttaaaatgc cgtctgaaag cctttcagac ggcattttgt ttggtttgga
     8941 aaatacttat gccgttcttt ccctgaaaga gagaatccaa aaaaccaaag ccacaggaat
     9001 ttatcggaaa tgacaaaaac ccgacgaacc ggattcccgc ctgcgcggga atgacgaatt
     9061 agaagttacc cgaaatttga aaaaacaaaa tccaacccaa cagaccgggt tttcgtttgc
     9121 acggaaataa tgcaataaat aaagaaaata taaagtattt gaatttacta tattattttt
     9181 cagcttcttc aaagccgacg acttcgaggc cgaagccgat gaggccggtg aaggatgacg
     9241 gctggccgag gacgcgcagt tttttgacgt tgaggccggc gaggatttgt gcgcctatgc
     9301 cgtagctttt gctgtcccat ttgtaggctt ggtttgcgcc tttgggcagg gtgcggtcga
     9361 gcagggatgc gccgtcttcg gcgcggtgca ggaggatgac tacgccgctt tcggcttgtt
     9421 ggatgcgctc aagggcttta ggcaatgacc aggaatggcg cgggttggct tggatgaagt
     9481 ccatcacgct gaagggttcg tggacgcgga cgagggtttc ttcgtcggcg gttggtgtgc
     9541 ctttgacgag ggcgaggtgg gtttcgccgg agagtttgtc gacgtaaacg tgttgttgga
     9601 actcgcccca cggggtttgt acgggtgcgt tgcccatgtc ttcgagcagg ctttcggtgc
     9661 ggctgcggta ttcgatgagg tcggtaatgg tgccgatttt gaggttgtgt tcttcggcga
     9721 acttcatcag ttcgggcata cgcgccatgg tgccgtcgtc gttgatgatt tcgcagatga
     9781 cggcggcggg aatcaggccg ttcatttgtg ccaggtcgac gccggcttct gtgtgtccgg
     9841 cgcggacgag tacgccgcct ttttgggcgc gcagtgggaa gatgtggccg ggttggacga
     9901 tgtcttctgg ttttgcggat ggtgaaacgg cggtttgaat ggtcagggcg cggtcggcgg
     9961 cggaaatgcc ggtggtaatg ccgtgggcgg cttcgatgga gacggtaaag ttggtgccgt
    10021 attgcgcgcc gtttttttgg gtcatcatcg gcagcccgag tttttcgacc atttcgccgt
    10081 ccatcggcag gcagaccaag ccgcgcgcgt gtttgatcat gaagttgatg gcttcggggg
    10141 taacgaattg cgccgccatc agcaggtcgc cttcgttttc gcggtcttcg gcatcggtga
    10201 tgatgaccat tttgccggct ttgatgtcgg ctaggatttc ggggatgggg gaaatatggg
    10261 acatggtgtg ttttcctgtg tgttcgggcg ggcgggcggt gtgttcggta tcgagccaaa
    10321 aggcgggcat ttttgcagcc tgttcgattt tacgggcttt tttctcgccg aaggatttgt
    10381 tttttaaaag ggcggagagt tcgcccgtat tgaggcagac ggcttcggca aaacgggttt
    10441 gcaaaccgcc gtaatgtttt tctatccact gccgcaggtt gcggcggcgc aggtcggctg
    10501 tgtcagtcat tgtttttccc tttgcggaaa ccgaatgtag cggattgtaa tctcgtattt
    10561 accaaaaagc aaatctgttt tttcgccaaa ccgaattatt tgctttttgg taaacagatg
    10621 ccgtctgaag ccggattcgt ggcttcagac ggcattgccg caatccccgc cggcggggcg
    10681 gctgtttctt cccgcccgct tccgattcgg ggtttgccgg aagcttattc cgaaaaaaat
    10741 ttctgataaa cggccttgat tgccgggtac agcataggcg gtacgagcag gcgcaatatc
    10801 ggcaatccta tgatgaatgc cgcctttccg ataaaaatcg gacggattgc cttcgcgccg
    10861 aatatctttc tgcatttttc tttaaatgaa aaatattctc ggtagatgac gataatgttt
    10921 ttggaaaaat aaagccctac caatatagac ctcagatagc cgatattgtc gaagacaaat
    10981 ttttgcaaac cgccgtagat gggggaattt ttgttttcga ctaaaaaatc cacaccccgg
    11041 tcgagatgcg tcagcacatc gctgaaattt ttgtatttga tgttgtggct gatagagccg
    11101 ccgagaacgc ggtattgata aaaagggtta tcgtaaaagg caaaagtctt gacaaaacgc
    11161 gccaattgca aactgtacgg aaaatcctcg tgaatgtatc cttttgggaa aaacagattg
    11221 tttttgatga ttatttcccg cctgacaatc tttgtccacg cgttggcgat atagtaccgc
    11281 ccctccacca gcgtttcaaa atggcggaca aaatcattat ccgaaaagtc cgcccctttg
    11341 gggatgtcgc ggtaattgaa ggacgaggga tgcaggatca aatcaacctt tttgtctgca
    11401 agttgttgta aatcaaagag aatccccccc ccccgcgttt tttgaacggt tggtatcggc
    11461 ccaatagtcg tcgctgtcca aaaagatcag gtaatcgcct tttgccgctc ggataccggc
    11521 gttgcgggca tccgacagcc cgccgttttc ttgatgaatc acttttatat gcggatattt
    11581 gcctgcatat tcgtcgcaaa tcttcccgca gccatccggc gaaccgtcat cgaccaaaat
    11641 catttcataa tcggcaaaat tttcggcaag cacggaatcc acgcagcagc ggaggtattt
    11701 ttccacattg taaataggga cgatgatgga gaaaatcata aatatcaatc cattatatta
    11761 agatgtttgc gcgtatgcct caaacccgca cccgcaatgc gtttgcatcc gcaccctgta
    11821 actttataaa acttattgct ttatcaaggt atggaaacct gtttcccgaa aggcggcgca
    11881 ggatgcccgt tccctgcatc tttgccttat tccgacatca gatgccccag cttgtctgct
    11941 ttggtttgga ggtagcgttt gttttcaagg ttttccccga cgtgcagggg gatgcgttcg
    12001 accacgttaa tccccgcatc tttcagggtt tggatttttt cggggttgtt ggtcagcagt
    12061 ttgaccgagc ggatgcccag atattcgtag atagattgcg ccaaacggaa atcgcgggca
    12121 tcgacgggca gcccgagtgc caaattggct tcaaccgtat ccataccttg gtcttgcaga
    12181 tgataggcgc ggattttgtt aatcagcccg atgccgcgtc cttcctgacg cagatagacg
    12241 atgatgccgc gcccctctgt ctgtacggcc ctcatggccg cttcaagttg aggtccgcag
    12301 tcgcattttc tcgagaacag cgcatcgccc gtcaggcatt cggagtggat gcgcgtcaga
    12361 accggattgc cgtctgaaaa atcaccgacg gtcagcgcga cgtgttcctg cccatttgcc
    12421 tcttcaaagc cgtgcatcgt aaatacgccc cattcggtcg gcaggcggca ggatgccaca
    12481 tggttcaaca tttcagacat cttcactccc atcttttccg gcagacatac ccaacagcag
    12541 tttcaaaggt tcggacaaag ccaaggcaag agccacccat tccacctgtg ctgccgtacc
    12601 cgcacactct gcgcattcaa attccacatg aaccacgccc aacacaccgc cactttccgt
    12661 gcagaccgga atggaaattt gcgccgccga agcatgattg cgttctcccg aaagctctcc
    12721 caaatccaac caacgacgca catccgaggc aatattcatc cagccgcttt gcgccgaacg
    12781 gcaagccagc gaaaccttgc ctgccgcttc atccaaatcc cacaggtttt ccaaaacctc
    12841 gccctgccgg gacaggcaaa ccagccgaaa ggcggcattt tcagacggca tcagggcata
    12901 aacggctgcg gcgcgcacgc ctttacagcg tgaaaacacc gaatccaaag ccatgaaaag
    12961 ccgtttcaga ccttcctcat tcccgctgtt ttgctccaaa taatcggcaa gcctccaatc
    13021 ctcatgctca agccacaaaa ccgaacgctc aatcgaagcc gtccccatat ccattaccgt
    13081 ctgcgccgtc agatactccg cccgaacctc gtcaagcggc agcttcaaac cctgagtcag
    13141 cagaaaatcc ttaatcaaag cagcaggcat acccaaattc cgtcaataaa aacaaaaaac
    13201 cgcccgattc cgatgtcagg cggacgaaaa acagatttta ccgccccaaa ccccaagcca
    13261 tcaacagccc cgccgaaaac cttgcacaaa aaaacaaaat ctgtataatc gtccaattcc
    13321 catgcggacg tggcgaaatt ggtagacgca ccagatttag gttctggcgc cgagaggtgt
    13381 gagagttcga gtctctccgt ccgcaccaat aatgataaat aaattatata gttaatgctt
    13441 atcgggacaa attagtgcca caaaacataa gtcgtctgct tttcagcggg cggctttttt
    13501 atctgcctat atgtttcaac acgcagaacg gtacataaag tgccgccatt tgttccgcac
    13561 cgaatcggaa acgggtgctt ccgcccgcaa tcagattcac cgcccctaat gccggggttt
    13621 caactggaaa gaagaaaata ttgattttga tgatggcatc aaaaaacccc ttccgcccgt
    13681 ctgccaaaag gaatggttaa gaaaagcaat gcgtcttttg atggaacgcg atatataagg
    13741 aattgagggg aagggcaggc gggtttctgc ccgttaatcc tgcggacgat cccgccaaac
    13801 ccgacacgcg cgggcatatg gcggattgtt tacaatattg tctaaaaatc tgaacctgca
    13861 aacgtgaaat aaggtagaat acgccccgtt attttaccgt ccaatcccca tttaccaagg
    13921 aaaaatgatg agtacttcat tgagttaccg cgatgcgggc gtcgatatcg acgcaggcga
    13981 ccaactggtc gaaaacatca aaccgtttgc caaacgcacc atgcgtccgg aagtattggg
    14041 ggatttgggc ggttttggcg cattggtcga aatcggcaag aaatataaaa atcccgtatt
    14101 ggtcagcggt acggacggcg tgggtaccaa gctcaaactt gcctttgatt gggataaaca
    14161 cgatacggtg ggcattgacc ttgttgcaat gagtgtcaac gatattttgg ttcaaggagc
    14221 agaacctctg tttttcttgg attatttcgc ctgcggcaaa ttggatgttc cgcgcgcaac
    14281 cgatgtcatc aaaggcattg cacaaggttg cgaagaatcc ggctgcgccc tgattggcgg
    14341 ggaaactgcc gaaatgccgg gcatgtatcc cgtcggagaa tacgatttgg cgggttttgc
    14401 cgtcggcgta gtggaaaaag agaatgtcat taccggccgc agcatcggcg cgggcgatgt
    14461 ggtattgggt ttggcttcca acggcgcaca ttcaaacggc tattccctta tccgtaaaat
    14521 catcgaacgc gacaatcccg atttggatgc cgagtttgat aatggcaaaa ccttgcgtga
    14581 ggctgttatt gcgccgaccc gtctgtatgt gaaaccgatt cttgccgctt tggaaaaatt
    14641 taccattaaa ggtatggcgc acattaccgg cggcggcatt accgaaaacg tgccgcgcgt
    14701 gttgcctgaa aacacggttg cacaaatcga tgctaaatcg tgggaattgc ccaagctctt
    14761 ccaatggctt caaaaggcgg gcaatgtgga aacccaagaa atgtaccgaa cctttaactg
    14821 cggcatcggc atggtcgtta ttgttgctgc cgaagatgcc gatgcggttc agggtctctt
    14881 gggtgaacaa ggtgaaacag tttaccgttt aggtttgatc cgtgagcgtc agggagacga
    14941 gcatcaaacc caggttgcct gattgcttct atagcgaaat gccgtctcaa acatgggttt
    15001 ggacggcatt tttatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag
    15061 tacagatagt acggcaaggc gaggcaacgc cgtactggtt tttgttaatc cactatatat
    15121 tgattgatat gagggtgggt tacacatgct taaacgatat aagccattgt atatttatgg
    15181 gattcggaat ggaacatttt accctgcatt agtatttccg ctttttatta tgccgtctga
    15241 aaatatcatg atgatacgtt ttcagacggc atagctgata ttgcagtatc agataggggc
    15301 aaacggaaaa cactaaaccg aacaatatta ctgctatgcc aagcgtactt atgggggcat
    15361 aaacttacat gcttggtctg ttatccgaat gaccgtacgg ggattaaaac ccctaatatg
    15421 ccgtctgaac ccgccttatt ccagcacggg ttgggggacg ttgtgccgga tggcgtaaac
    15481 agcggcctgc acccggctgc tgaggttgag tttgcggagc aggttttgaa cgtggacttt
    15541 gacggtggat tcggcaagat cgaggtggcg ggcgatgatt ttgttgctgt gtcctgcggc
    15601 gagatagccc aagatttcca gttcgcgagg ggtaagtgag gagagtgcct gtgtcccttg
    15661 ggcaggttgg ggggaaatca ggcttttaac gagtttggcg gtcatctcgg gcgagaatac
    15721 attatcgcct tcggcggctt tgcgtatgct ttcgagcaga aagtcggcgt tgatgttttt
    15781 cagcaggtag ccgcgcgcgc cgatgcgcat acattcggtc aagtcgtcgt tgtcttcgga
    15841 aacggtcagc ataatcactg cctgctgcgg attgatgctg atgatttggg agagtgcttc
    15901 gcgtccgttc ataccgggca tatcaaggtc aagcaggacg acatcgggtt gcagccggtt
    15961 gatcatttta atgcccgaga ggccgtctgc ggcttcgccg atgacctcaa aatcatgttg
    16021 gcgcgacaaa agtgccttga tgccgctgcg gaaaagggta tggtcgtcta tcaggataat
    16081 tttaatggtc atttcaagct ttcttcagat acacccgcca atgagacggt ggttccctgt
    16141 tggggttggg aatagatttt caatacggcg cggatacgtt tggcacgctc ctgcatgatg
    16201 tgcagtccga catggctgcc cgtgggttct cctattttct ccgtgtcgaa accttgtccg
    16261 ttgtcttgga tggtcatggt aaagcgtccg ccgtgttcgg aaagggtgaa ttttacatgg
    16321 gtggcgcggg cgtgtttgcg gatgttggac aggctttctt gcaggataaa aatcatttgg
    16381 agctgctcgt cctgtgtagg caggtgcgtg ccgttttccc aaacggtttc gacagtcgtg
    16441 ccggtctgtt gcgtgaagcg cgcgaatagg tcggcaacgg cttcgggaaa ttctttatta
    16501 ctgattttgg tacggaagtt gagcagcagt tcgcggacat cttcataaca ttcctgcacg
    16561 cctgttttga tgaagccgat gttttctgcg gcttcctccc gtttgttttc ggcaaaggcg
    16621 gtttccagca tctgtacctg taggtttagg aacgttaatg cttgtgcgat gctgtcatgt
    16681 aatccttgcg caatcaggtt gcgttcctgc aatactgcaa gcaggcgttt ttcttcctcc
    16741 tgttttgcgc cggcaagcga tacgcccaat tgcctgccta gtgtttgaag caggatgcgg
    16801 tcgtcttcat caagagaaat gccgtttgga aagctgagca acagcctgcc caatgtttcg
    16861 ttctggtact caatggggaa gatttcctca tggtacttcc ccaaatccga agctgctgtg
    16921 ccgcaatccg catgatgaat ggaaacataa acatcggatc cgccgtccaa acaaactctg
    16981 ccggaatctg ctcctacggc gggcaggata cggtttagaa aatgttctgc agcctgttgc
    17041 ggtatgtagg attggtgcag gtcccgtgta gtttggtaca gcagggtcag gttttgattt
    17101 tgtttttcga gactgcgtgt ctgctcggcg acttgtcctt ccaaatcatc atataaaatt
    17161 ttcaacctgc cgcccatttg attgaaacaa cgcccgacct gtttgaattc cggcgtaccg
    17221 ccttcgggaa ccggaatatc gaaacacctc cgtccgatgc gttccgcacc ttcccttaac
    17281 gcctgcagcg gccggataac ccaaatctgg tgccaaaaca gcatcagtac agacgacacc
    17341 agcgtcatca acataattgc ccattgaaaa cgcctgagcc accatgtgtt tttttcgttg
    17401 gcattttcca atgcctgcaa aaacagttcg atgtttccgg caaagcggta gagatcgacc
    17461 tgagtcggtc gccggtagga ctggagcggg gggaggatgt gtgcctgcca atctataatc
    17521 agcatggatt gtatcaaatc ataagcaaga ggggtgtccg aaggaatcag cggatggatg
    17581 gcatcacttt gtgaaatacg attcaggctt ttttcaaatt cgtcaacctg attgtctatt
    17641 tgggagcggg gagatccttc acccgccatg tatgccagac ggtatgcctg cattctcaag
    17701 ttgcccgcct cttcgatgac ggaggccgcg ttttccagac gcaaagagag cagcagtgtc
    17761 aaaacgacag acaatgccgc caacccgacc cacagtccgg tcaggagttt caggcgtagg
    17821 gaaaggctga tgccgtctga aaaacgggct ggcagtatca tgctcggcga aaaattgttc
    17881 taaataatgc aaacaatatc attctttggg attagataca actgctcaga aagaattggt
    17941 taaggaaaac ttaatccgca ccgcttcagt gttaaatcat gttatgataa aattcagata
    18001 atatgaattt atttgccgtc tgcttcagac ggcattttga gtcattggga acggcagact
    18061 gcaatcatga aaacctcgaa cttttccaag agaacatccg caatgaaaac ttttgccctg
    18121 atactggccg acggtcaggc gagccgtatg ggaggcgagg acaaggggct tgctcttttg
    18181 gggggtaagg cactgataga tcatgtcatc gacagggtca ggccgcaggt cagccatatc
    18241 gccatcagca ccaaccggaa tttggaagaa tatgctcgaa gaagtccgca tatttttccg
    18301 gacgcacggc agtggcagca tttcggcccg ctttcggcat tgtgtaccgc agccaacgat
    18361 ttgcagttgg cgactgccga ctggcttttg gttgtgccgt gcgatatgcc gtatctgccg
    18421 ggcgatttgg tggcgaggtt tgaaaccgtg tcgaaacgca caccgttgtg caatgcgtat
    18481 tatgtggaaa cgccgataac gatgcactac aacattatgt atatccgccc gcagattctg
    18541 caaagcgcga ttccctatct gttttcgggt atgaaaacat taagaagctg gttgcagcag
    18601 caaagggcgc ggccggtcag attcgagttt gacgggcatt ttgccgactt gaacacgcaa
    18661 atcgatttgc aggagggata agggcaagac cgccgaccgg cgtggaaagg aaaggtcaag
    18721 ccataccggg cggtttttgc ccgaatcgga ggcataatgc tgtctgaagg catttcagac
    18781 ggcatttttc gcggggagat gctacaattt gcaccatttt taccgacaca gggaaacagg
    18841 attatgttta caggcattgt tcaaggattg ggaaaactga cggcaatcca ccgcccgtcg
    18901 gaggcatttc acacttatgt cgtcgagctt ccgcaagagg cggcggacaa tctgcaacgc
    18961 ggcgcatcgg tcgccaataa cggctgctgc ctgacgatta ccgaaatcga aggaaaccgc
    19021 gtcagtttcg atttaatggc ggaaactttg gcaaaaacca atttggggct gctgaaggaa
    19081 ggcgattgcg tcaacatcga acgggcggcg cgtttcggag acgaaatcgg cggacacgtc
    19141 atgagcggac acattatggc aaccgtgcct attgtcgaaa tcgaacggga cgggttcaac
    19201 cgcacggttt ggttttcgct cccgcatgaa ctcaaaccct atatcctgac caaagggttc
    19261 gtcggcttgg acggttgcag cctgaccata ggcaaagtcg aagacggccg tttcaatgtc
    19321 catctgattc ccgaaacttt ggaacggacg ctgttcggca gcagaaaggt cggcgacagg
    19381 atcaacatcg aaatcgatcc gaatacgcag gcaatcgtcg ataccgtcga acggctgatg
    19441 gcgcaaagat atgcaaagtg aggcagagat ggaattgaac gaatttctcg ataaagccta
    19501 tgccgttttg cggcgtttgg atgccgtgct tccgcccgaa cccgggcata cggattggaa
    19561 cgcgcttgcc ttccgctggc agagtgcggg caaaaaaggt tttttggaac acttgcccga
    19621 tccgcacacc tttcccttgg taaggctggc gggagtcggc aggcaaaccg aattgctggt
    19681 gcgtaatacc gaacagttca ttgtcggcag acccgcgaac aatgtattga tgagcggcgc
    19741 gcgcggaaca ggcaaatcct cgctggtcaa agccctgcta cacgaatatg cggataaggg
    19801 attgcgcctg atcgaagtcg ataaaagcga tttaatcggc ctgccttacc tgttgacgct
    19861 tttgaaggaa cgtccggaaa aatttatcgt attttgcgac gatttgtcgt ttgaaagcgg
    19921 cgatgaaacc tataaggcat tgaaaaccgc gttagacggc ggtctgtctc aacgttgcgc
    19981 caacgttttg gtttacgcga cttccaacag acggcacctg atgcccgaat attttgacga
    20041 gaatgccggt acgacgggga tgcgggggga aatccatcag aaagaagcgg tggaagaaaa
    20101 aatatccctg tccgaccgat tcggattgtg gctcagtttt tatccgttcg atcaaaacga
    20161 ttatctggcg gcggtgcaaa attggctgga agatttcggc gtgccgtatg atgaaaccgc
    20221 acagatggcg gcattgcagt gggcgcagac gcggggcagc cgttcgggac gttccgcgtg
    20281 gcagtttgcc tgcgactggg cgggcaggct gccgaaacaa cgggcgttgt gagcgtttcg
    20341 gggattattt gaggcaggaa gaaagccaca tttgctgctg ttgcggtgtg aggatatgga
    20401 agaaccggtg ttggatttcc aattcgtcca ccgcaaaatc cataccggac aaatagcggc
    20461 tttcgacata atcgcgcgcc tcgttccgat taaaaacatc cgaggaaatg atttcgacga
    20521 cagaccggcg gcggctgtgt tcggaatgca taaccttcaa acgcgccctg tcgcccgcca
    20581 ttttgaaggc ggtgcggatt ttacgcagct cattgtgctg actttgggtc agcccgagtc
    20641 ggcgtatgtc gcagttcggt tgaaaatcgt tcggggggat ataagccgtg gcggcgtggg
    20701 aaagctgaca ggaaagcaaa gccatactta aaaaagaggc ggcaggtttg gcaaaacggc
    20761 agggagcagg cagtggcacg gcttttcctt taatccagat atttcggaca atatacttga
    20821 ttgccgtgtg aaagatggta ttatggcagt taacttttgt taataatgct gtttcggggc
    20881 tttcccgcgt tgagtccgcc cgtaaaggca ttgaaggaaa aaatatgacc gcctaccgta
    20941 tcgcacccag tattttgtcg gcagactttg cccgcctcgg agaagaggtg gaaagcgtca
    21001 ttgcggcagg tgcagatctg atccattttg acgtgatgga caaccattat gtgccgaacc
    21061 tgaccttcgg ccctatggtt tgcgcggcgt tgaagcctta tgcaagcgtg ccgatcgatg
    21121 tgcatttgat ggtcgaaccc gttgacgacc tgattcagtc gtttgccaaa gcaggagcat
    21181 cgattatcac ttttcatccc gaagcgagcc gccatatcga ccgcagcttg agcctgattc
    21241 gtgatatggg ctgtcaggcg gggctggtgt tgaatccggc aacgcccgta tatctgttgg
    21301 aaaacgtatt ggacaggctg gatatggttt tgctgatgtc ggtcaacccc ggattcggcg
    21361 gacaaagctt catcccgcac acccttgaaa aaatccgccg ggtgcgggcg atgctggatc
    21421 ggtacgaagc acaaagcggg cggcacatcg ccatcgaagt ggacggcggc atcaaaaccg
    21481 acaatattgc cgccgttgcc cgagctggtg cggatacctt tgttgccggt tctgcgattt
    21541 tcggcaaacc agactataag gcggtgattg atgcgatgag ggcggaactg gaaaaaagcg
    21601 gcaggctgaa ccttgcgcca atgattgaaa tagaaatgcc gtctgaaaat tagctctgtt
    21661 tcagacggca ttgcgttatt tttcgacggg cagcccgaaa tgcaggtagg cgcgttcggt
    21721 cgccatcctg ccgcgcgggg tgcgttgcag gaagccttgt tggataaggt agggttcgat
    21781 aacgtcttcg atggtgtctg tagattcgcc gatggcggcg gcaacattgt ccaaaccgac
    21841 cgggccgccg ccgaatttgt gcaaaacggc ttcgagaaat ttcctgtcca tcacgtccag
    21901 cccctgcgcg tccacgtcca gcatacttaa agcggcatcg gcgatgccgc cgtcgattgt
    21961 gccgttgttt ttcacgtcgg cgaaatcgcg cacgcgtcgc aacaggcggt tggcgatgcg
    22021 cggcgtaccg cggctgcgtt tggcgatttc ttccgcgcct tcttcggaca tatcgagctg
    22081 caacagttgt gccgaacggc tgacgatggt ggtaaggtct cggttttcgt aaaactcaag
    22141 gcgggagacg atgccgaagc ggtcgcgcaa cggattggtc agcataccgg cgcgggtggt
    22201 cgcgccgatg agcgtgaagg gcggcaggtc gattttgacg gaacgggcgg cgggtccttc
    22261 gcctatcata atgtcgagcc ggtagtcttc gagcgcggga tagaggattt cttcgacaac
    22321 agggctgagg cggtggattt cgtcgatgaa caatacatcg tgcggatcaa ggttggtcaa
    22381 aagggcggcg aggtcgcctg cgcgttcgag gacggggccg ctggtttggc gcaaatttac
    22441 gcccaattct ttggcgatga tgtgcgccag tgtggttttg cccagtccgg gcgggccgaa
    22501 gagcaaaacg tggtcgagtg cttcgccgcg ttttttggcg gcttggatga aaatggcaag
    22561 ctgttctttg gctttgtctt gcccgatgta gtcgtccagc gttttggggc ggagggcgcg
    22621 ttcgagcagt tcttcctgtg cggaggcggt ttgggcggca acgatgcgtt gcggttgcgc
    22681 ggcggtcagg ttgtcggttt gcagcatagt gttccctttg tcgggtatgc cgtccgaacg
    22741 gtcggcggcg tttcagacgg cattgaaaag ataacggtca aaagcgtttt atccgcgggt
    22801 ttcgcgccaa acgagatagt cgcgcagggg cggcataggc gggttgatgc agtgggggca
    22861 gatgccggtg gtgtcttcgt cgtcgctgtc gatgtggtag gcgttggggt cgaagcggat
    22921 ttgctgcatg acggcttgcg ccaatgcctg cgcctgaaca acgtcgaaat tgaatttttc
    22981 gaggatttcg ttcaacaggc ggatttcgcc accgctaaat tccgcactgt gtttgaggat
    23041 gaggcgttga taatcttcgt tgttgatttt aggcaataaa tcggttttca gggtcatgat
    23101 tttatgtcgg gggcaggatg gctgaagcgt gaattatagc ggatatggca gcggctgttg
    23161 caaagcttgg attgggtatc gtcctcaaaa ctttatagtg gattaacaaa aaccagtaca
    23221 gcgttgcctc gccttgccgt actatctgta ctgtctgcgg cttcgtcgcc ttgtcctgat
    23281 ttttgttaat ccactataaa agtggctgca ttggacgttt taatgttttt ctgttttgtc
    23341 ttgcgccgct cgaaacgttt tttgcaatgg ggcgatttgt gccagccgcg cccggtcgat
    23401 ttgttcggcg ataaggtgcg cttttccctg cgcgcggcat tcggcggcga ttttgcccga
    23461 atccgcctga ttggcggctt cgagcagggc gaaccagtgt gcgcgttgcg ggtagggcgt
    23521 gtgttcgcgg ttcagacggc cttgcgtgtc ggcaatgcag acgttcaatg ccgtctgaaa
    23581 gcgttcggga cggcggaagg catcggtttt tttcaatacg ttcagaatgg tttggctttt
    23641 aagctgtccg acttggtgga aaataatgtg ccaacggcaa accaattcgg caagctcggc
    23701 gcaatgtttc ggcgcacgca gccgctgatt gacttcgcgc acgggttcga caccggcgag
    23761 gtcgtgtccg tggtggcgcg gcaggatgtc ggacggtgtt ttggctttgc ccaagtcgtg
    23821 cagcagggcg gcatagcgtt cgggcaggct caaacccata tcggcggcgc gttgcagcgt
    23881 catcagggta tggatgccgc tgtcgatttc gggatggtag tcggcgcgtt gcggcacgcc
    23941 gaagagggca ttgacttcgg gcagcaagac tttgagcgcg ccgcattcgc gcaacacttc
    24001 aatcattttg cgcggatttt tttccatcaa acctttcgca aactcctgcc agacgcgttc
    24061 ggcaaccaat gcgtccgctt cgccgttttc caccatctgc cgcatcagct ttatggtttc
    24121 ttcggcgatt tcaaacctgt aacgcgcggc aaagcgggca gtacgcagga tgcggacggg
    24181 gtcttcggcg aaggccgggg aaacgtggcg caaaatgcct gccgccaaat cccgttgtcc
    24241 gccgaaaggg tcgataatct tgccgtccgc atcttgcgcc atcgcgttga tggtcaggtc
    24301 gcggcgcatc aagtcctgct ccagcgtaac gtctttgtcg gcgtggaaac tgaaaccgac
    24361 gtaacctttg gcggttttgc gctcggtgcg ggcgagggcg tattcttcgt gcgtttcggg
    24421 atggagaaac acgggaaaat ctttgccgac cggctggaag ccttgcgcca gcatggtttg
    24481 tgcgtctgcg ccgacgacca cccaatcgcg gtctttgacg ggcaagccca aaagataatc
    24541 gcggacggcg ccgccgacga ggtaagtctg catatgcttc ctattttaaa gttatcaaca
    24601 atgccgtctg aagcggcttc agacggcatt gttccaaccg gcggttatgc tacgcctttt
    24661 ttctccaagt aactttcgta atcgcccaag tagtgttcat atccgccttt gccgtccagt
    24721 tcgatgattt gggttgccaa ggaggaaacg aactgacggt cgtgggagac aaaaatcagc
    24781 gtgccgttgt atttttccag tgccatgttc aaggattcga tgctttccat gtccatatgg
    24841 ttggtcggtt cgtccatgac taagacattg ggtttcaaca gcaacagttt gccgtaaagc
    24901 atacggcctt tttcaccacc ggagagaacc ttcacttttt tcacgacatc gttactgccg
    24961 aagagcaaac gccccaaagt gccgcggatg acttgttcgt cgtcgccttc ctgcccccat
    25021 tggcgcatcc attcgctcag gtccatatcg acgtcgaagt cgttttcatg gtcttgcgga
    25081 tagtagccga cactggcttt ttccgcccat ttgatggtgc cttcgtccgg taacaggccg
    25141 tctgaatatt cggggttgta cgcgcctgcc aagagtttca gcaaggtaga tttacctgcg
    25201 ccgttcgggc cgatgatggc aaggcgttgt cccgcttcga ggatgaagtt caggttttta
    25261 aacaactggg tttcaaagcg tttcgccagt ttttccactt ccacagcctg acggtgcaac
    25321 ttggcttttt cgtcggcttc aaaacggata tacgggtttt ggcgggtgga aggtttgact
    25381 tcgaccatct ccgatttgat tttgtctgcc tgtttcagac ggctggtcgc ctgacgggct
    25441 ttggatttgt tggcactgaa ccgcgccaca aattcttgca attcctgcaa tttttctttc
    25501 gccttggcat tgtctttcag ggcgcgctca cgcgattggg cagaagcgag catgtaatcg
    25561 tcgtagttgc ccggatagat ggtgatagtg ttgtagtcca aatccgccat atgggtgcag
    25621 acttcgttca aaaagtggcg gtcgtgcgaa ataataatca tcgtagaatc atattggttc
    25681 aacacgcctt ccaaccagcg gatagtgttg atgtccaagt tgttggtcgg ttcgtcaagc
    25741 agcagcacat ccggcttgga gaacagggct tgcgccagca atacacgcag tttgaagccc
    25801 ggggcaactt ctgccatggt cgcattgtgc aaatcttcgg aaatgcccac gccgctcaac
    25861 aattcggcag cgcgtgcttc ggcggtgtag ccgtcgtatt cggcgaactt ggcttccagt
    25921 tcggcggctt tcatgtaatc gtcttcggtg gcttcgaggt tggcgtaaat cgcatcgcgt
    25981 tcggtcatcg ccgcccacat ttcggtatgc cccatcatca ccacgtccag cacgcgcatc
    26041 tcttcgtagg caaactggtc ttggcgcaat ttacccaaac gcacgccgtt ttcaatcgcg
    26101 acttcgccgg cagtctgttc caaatcgccg ccgaggattt tcatgaaggt ggacttgcct
    26161 gagccgttgg cgccgatcaa gccgtagcgg ttgccttcgc cgaatttgac ggatacgttt
    26221 tcaaacagcg gcttcgcgcc gaactgcatg gtaatgccgt tggtagaaat catgattggt
    26281 aaagccttta ttgaacaaga gattaaattt ttcgaatttt cggattgtaa cataaccgcc
    26341 ccgccgtctg aaacagacgg gcaaattttc agacacttat agtaaaatat ccgttttatt
    26401 atttcagaca aaaggtttcc aaatgatagt ccttcacggc atcccaaatt gcgatacggt
    26461 caaaaaagcc aaaaacaggc ttgccgaata tggtatggaa tttgaatttc gggattttaa
    26521 aaaacaggtg ccttccgaag cggaaatctg ctcgtggctg gaacaagtgc ctttggcaac
    26581 cctgctcaac aaacgcggga caagctggcg caaactcgat gccgaaacac agcaaaaagt
    26641 gctgtcctcg acggcggagg ccgtcaaact gatgtccgaa atgccgagcc tgatcaagcg
    26701 tcccgtattg gagtgcggcg gcaaggttta cgccggcttc agcgaagaaa cctacgacgg
    26761 catcttcaac cggcaagccc tgtgcagaca gggataaaaa ccgttacaat acccgacttg
    26821 aatttcccaa tcccatttcc aatctgaaaa tatgttccat tccatcgaaa aatacagaac
    26881 gcccgcccaa gtccttttgg ggctgattgc attaaccttc gtcggcttcg gggtcagcac
    26941 ggtatcccat ccgggtgccg actacatcgt ccaagtgggc gacgaaaaaa tcagcgacca
    27001 ctccatcaac aacgctatgc aaaatgaaca ggcggacggc ggcagccctt cgcgcgacgc
    27061 ggtgttccaa tccctgctgc aacgcgccta cctgaaacag ggcgcgaagc tgatgggcat
    27121 ttcggtttct tccgaacaaa tcaagcagat tatcgtggac gatcccaatt tccacgacgc
    27181 aaacggcaaa ttcgaccacg cgcttttaaa ccgctacctt tcccaacgtc atatgtctga
    27241 agaccagttt gtcgaagaaa tccgcgatca gtttgccttg cagaatttgg taaacctcgt
    27301 ccaaaacggc gtattggtcg gcgacgcgca ggcggaacag ctgatcaggc tgacgcaggt
    27361 caaccgcacc atccgttcgc acactttcaa ccccgacgaa ttcatcgccc aagtcaaagt
    27421 gtctgaagcc gatttgcaga agttttataa cgcaaacaaa aaagactacc tgcttcccaa
    27481 agcggtcaaa ttggaatatg tcgccttgaa tctgaaagac tttgcagaca aacagaccgt
    27541 cagcgaaaca gaagtgaaaa atgcgtttga agagcgcgtg gcgcgtttgc cggcaaatga
    27601 agccaaacct tctttcgagc aggaaaaagc cgccgtagaa aacgaattga aaatgaaaaa
    27661 ggcggttgcc gacttcaata aggcaaaaga aaagctgggc gatgacgcgt tcaaccatcc
    27721 ttcctcgctt gccgaagccg ccaaaaacag cggtttgaaa gtcgaaaccc aagaaacttg
    27781 gctgagcagg caggatgcgc aaatgtccgg tatgcccgaa aacctgatca atgccgtttt
    27841 cagcgacgac gtattgaaga aaaaacacaa ttccgaagtg ctgaccatca acagcgaaac
    27901 cgcgtgggtc gtccgcgcca aagaagtccg cgaagagaaa accctgccgt ttgccgaagc
    27961 caaagacgcg gtacgtcagg cttatatccg taccgaagcc gccaaacttg ccgaaaacaa
    28021 ggcaaaagac gtgcttaccc aactgaacgg cggcaaggct gttgacgtga aatggtcgga
    28081 agtgtccgtt ttgggcgcac agcaggcaag gcagtccatg ccgcccgagg cttatgcgga
    28141 actgctgaaa gcaaaaccgg caaacggcaa acccgcctac gtcaggctga tcggtctgcc
    28201 ggcacccgtg attgtcgaag tacaggctgt aaccccgccg gatgatatcg ccgcacagct
    28261 tccgcttgca aaacaggctt tggcgcaaca gcagtctgcc aatactttcg acttgttgat
    28321 acgttatttc aacggcaaaa tcaaacagac caaaggagcg caatcggtcg acaacggcga
    28381 cggtcagtaa ttgacacttt tgttgacaaa ataacggtcg gaatattccg gccgttttcc
    28441 catacggcgg aatatgatga gccacaaaaa acaagatgcc ttccaaggat tgatcgacgc
    28501 gctgaaggtt ttacccaacg tcgggccgaa atcggcacag cggatagcgt atcatttgct
    28561 ccaacacaag cgcaaagagg ctgaaaaact ggtggatgcc ttgcagacgg cattgaagca
    28621 ggtttaccat tgcgcgatgt gcaacacgtt ttgcgaaggc ggattgtgcg atatttgtgc
    28681 cgatgaaaca cgcgacgggc ggcggctgat ggtggtgcat atgcctgccg acgtgtcgaa
    28741 tatggaagcg gcaaactgcc acgacgggct gtatttcgtc ctgatggggc aaatcaatac
    28801 ggcattggga atggacgtat ccgccatcgc attggacagg ctggcgcaac ggctgggcgg
    28861 cggggaagtc gaagaaatca ttattgcaac cgcttttacc gcagaaggca atgcgacggc
    28921 gtatgtcctg tccgagtttt ttaaaaacct gccttacaaa gtcagcaggc tgtcgcaggg
    28981 cattcccttg ggcggcgaat tggaatatgt cgatgcggga acgctggcgc aggcggtgta
    29041 cgaacgccgc ctgattaaag aaggcggggc ataacgccgc caatgcaaaa tgccgtctga
    29101 agccttcaga cggcattttt ccgggcggtt tacttgcagt ttagcccttg aatctgcaat
    29161 gcgcctccgt tgccgacttt gaacacgcac tgcgtcgcgc tgctgccgga cacggtaatg
    29221 ctgtcgctgc cgccgttcag gctgataggt tccttaacac ccataccgac tgccgttgcc
    29281 gccagctttt gcgcgtcggc ttcggaataa ggcgtgttgt tggcatcgat tttgatttct
    29341 ttggcgtagg cagccgcagc ggcggataag gcgatggcgc acaaagcaaa agcagttcgg
    29401 atattcattg ttttcctcaa gaaacggttt ttctgaagga aaggataggg cagtttgacg
    29461 cgttgtgcaa gttttccgcc cgctctgccg ccccgtcccc cgcactcggg cgttcggacg
    29521 ctgcctgggt tttacggtac aataaacttt tgccgccgcc cccaaaccgc cgtccggggc
    29581 ggcacgtttg cagacatttt taaggtagcg ttatgttttc tctagaggct tggataggct
    29641 tgaggtatct cagggcgaaa aagcgcaacg gctttatgtc gtttatcacg atggtttcga
    29701 ttgccggaat cgccttgggc gtaaccgcgc tgattgtcgt cttgtcggtt atgaacggct
    29761 ttcagaaaga aatacgcggg cagctcctga atgtcgcgcc gcacgccgaa atcggctata
    29821 tcgacaatac ggatacggat tggcgcaacc tgcttcggtt taccgaaaac cgcaaaggta
    29881 ttttggctgc cgcgccctat gtttccaatc aggctctgct ggcaaacgcg ggcgaaatca
    29941 ggggcgtgca gatgcgcggc attttgccgt ctgaagagcg caaagtggtg gaatacggcg
    30001 acaaaatgcc ggcaggcaaa tttgaagatc tgattccggg cgagtttgac attatcctcg
    30061 gtgtcggctt ggcggaggct ttgggggcgg aagtcggcaa taaagttacc gtcatcacgc
    30121 cggagggcaa cgttacccct gccggagtcg taccgaggtt gaaacagttt accgtggtcg
    30181 gtctggttaa aacgggtgtt tacgaggttg acaattcatt ggcgatgacg catatccaag
    30241 acgcgcgcgt actgtaccgc ttggataagg aagttgcggg gctgcggctg aaactcgccg
    30301 acccgcaaaa cgcccccgcc ttgacggcaa cactgattcc ggaggcgcaa agggacgcgg
    30361 tttgggtgcg cgattggacg tacaacaacc gcagctattt tgaggcggtc gagttggaaa
    30421 aacggatgat gttcatcatc ctgacgctga ttatcgccgt ggcggcgttc aaccttgtct
    30481 cttccttggt gatggcggtt acggaaaagc aggcggacat tgcgattttg cggactttgg
    30541 gtctttcccc tggcggcgtg atgaagattt ttatggtgca gggcgcgttt tcaggctttt
    30601 tcggcacgct ggcgggtgtg gtctgcggcg tgcttttggg ttggaacgtc ggcagggtcg
    30661 tggcgttttt tgaaaacctg ctcggtgtcc accttatcaa ttcgcaggtt tattttatcg
    30721 actacctgcc cagcgatgtc gatatgggcg acgttgccct gattgcctgc atttccttgg
    30781 gactgtcttt cgttgccacg ctctacccga gctggcgtgc ctcaaaaacc caaccggcgg
    30841 aggctttgcg ttatgagtga gttgattttg aaatgcgaag gcgtgggcaa acgctaccgc
    30901 gacggcggtt tggacgttcg ggtgctgcac ggcttggatt tggaaatccg cgcgggggaa
    30961 agcaccggca tcatcggttc ttcgggcagc ggaaaatcga cgctgctgca tattctaggc
    31021 gggctggata tgccgtctga aggcagggtg ctgctgatgg gcgaggattt gcgtacctta
    31081 aaccagcggc gtttgggcga tttacgtaac cgccatctcg gtttcgtgta ccagttccac
    31141 catcttctgc ctgaattttc ggcactggaa aacgtgatga tgccgcttct gatcggcaaa
    31201 aaaagccgtg aagaggcagc agaaacggca atggcgatgc tggaaaaggt cggactgaaa
    31261 caccgttcga cgcaccgcgc gggcgaactt tcaggcggtg agcggcagcg tgccgccatt
    31321 gcacgcgcct tggttacgca acccaaatgc ctgcttgccg acgaaccgac cggcaatctc
    31381 gatcgtgcga acgccaggaa tgttttggat atgatgctgg aactgaaaac agaattggga
    31441 acggggcttg tggtcgtaac gcacgacgac gaactcgccg gccgcttcgg gcgcgtgatg
    31501 gtcatgaaag acggcaacct gcaccccaaa cagggcgcaa acgcctaaac agccgaaaca
    31561 acaccgcgcc gtctgaagcc ccacgccttc agacggcatt tcgacaagga atacaatgga
    31621 acggcagaca gatgaatttg cccaagcggc ggcaagggcg gcaatctggt ttttggcacg
    31681 ctatgcgggt tccagcgacg aagtgcttgc aaactgcacc gaacgcctgt ttcaggcatt
    31741 gcaaaacggt cattcgttta tccgtttgag cggtggcgag gctgacgcgc tgtcggcact
    31801 cgcacccgtg gtcggaacat ccgccgcgcc tttgattttg gaaggcagaa ggctgttttt
    31861 gggcaggatg tggcagttgg aatacgattt ggctgccgag ataaaacgct tggcggcagc
    31921 cgacacgtcc gcccccgacg cggcaggcgc gaggcaaaac ctcgcaaaat ggtttcaagg
    31981 cacgggcagc gaagggcagc gcgatgccgc cgccttggca ctgttgcagt cttttatggt
    32041 gattaccggc gggccgggaa cgggcaaaac gacaacggtt gccaaactgc tggcgctgat
    32101 ttgcggtgaa gacgaaaatc ttcccaacat cgcgcttgcc gcaccgacgg gcaaagcggc
    32161 ggcacatatg gcgcgcgcgc tgcaccgtgc aatcaacggt tttgacgcgc cggaggccgt
    32221 ccgccgccat ttgctcaaac tggaagggca aaccgtccac cgactgctga agctgcgccc
    32281 gcctaaaatg caggcggcgt tcgaccatat ccgtccgctg ccgttggacg tattggttat
    32341 cgatgaagcc tccatgttgg ataccgcatt gatgttgcaa cttttaaaag cggtcaaaac
    32401 cggcgcgcgc gtgattctgc tgggggatga aaaccagctc ccgtccgtcg gaatcggggc
    32461 ggtgctgtcc gttttgtcgc aaaaaaccgt tttggacgga gaaacgcacc aaaggctggc
    32521 cggcttcctt ccggaacacg gtttcagcgt cagcgcaaat ccgcccgtgt tggcgcaaaa
    32581 caccgcccat ctgtcgttca gccaccgctt cggcgacaac agcggcatcg gctgccttgc
    32641 ccgtgccgcc gtatcgggcg atgagggggc gtgggcattg tttgaccggt ttccggacga
    32701 actggaacat tcggaatgca gtccgaacgc tcgagtcgaa aggttgtacc gggcgcacaa
    32761 agcctattgg caggcggtaa aagacggcaa tatcgaagcc gcatacgcag gcatctcgga
    32821 tatcgtggtc ctggcagctt ggcggcagga tgcggaagac ttcaacgaag cctattgcag
    32881 ccatgtacgc cgcaaaatga acataccgga acatcaggcc tatttcgccg gagaaccgat
    32941 tatgatcaga cagaacgact acgcgcttga actgttcaac ggcgacatcg gattgattat
    33001 ggaagatgtc ggacggcagg gcagccttgc cgcctatttt gccgatgcgg acggatttaa
    33061 aaaggtagcg gtaagctgcc tgcccgaatt tgaacccgca ttcgccatga ccgtccacaa
    33121 aagccaaggt tcggaatacc gggaagtatg gctgctgccg ccttccgccg caccttcgga
    33181 cgaaggggac gatgcattgt ccggattgag taaggagctg ttatataccg ccattacccg
    33241 cgcgagagag aagttcgtat tcttcggcgg ggaagaagcc ttccggcaag ctgccgccac
    33301 cgtcaaaacg cgtcagacgg cattgggcag tatgctcgag cgggtatttt cacaagaata
    33361 atccgcccga atgatgccgt gccggcggcg cgggcgttcc gatgcctgcc gccggaagcg
    33421 caccgccgcc ccttatgcct ttttcaaacg gtacaggaaa gtagtttccc tgtttcgggc
    33481 aaaagccagc ggattgttcg gatcggcagc ttttttgtga accggtttgg ttttcaaaac
    33541 atccgcaacg cgcaaacccg cctgagcaaa tgccttatcc agaaaatcgg acggacgcag
    33601 gtgcagatgt tcggcaagat cggaaatgat caggcggatt tctccgtcgg gattcagatg
    33661 tttcggcgca tcccgcaaaa acgcagccag catcgcagat tcggggtcgt ataacgcgga
    33721 ttcgacggcg gaagtcggct tggcgggaag ccagggtgga ttgcagacaa tcagatcggc
    33781 aaacccttcg ggaaacagat cggtttcccg tatctcaacc tgtttttcaa agcccaaacg
    33841 ggcaatattg gcacgggcgc aggcgacggc tttcggattg gtatccgtgc cgatgacgga
    33901 aggaatgccc tgtttcgcca aaatggcggc aagcacgccc gagcccgtcc cgatatcgaa
    33961 tgccgtctga aaacccgttg acggcgcatg ggcgagcagg tcgaggtatt cgccgcgcaa
    34021 cggcgagaat acgccgaaag gaacgtgtat gctgccgccc agctgcggaa cggcaacccc
    34081 tttcttatgc cactcgtgcg cacccataaa ccccagcagc agattgagcg gtaggaaaaa
    34141 cggtttgccg tccgcctctc cgtacacgtc gagcagggcg gcgcgtatat cgggcgcgcg
    34201 tttgttgtcc aacacaaaac cggggcggat ttcaacggca agcatattca gaatacggct
    34261 ctgctgcgcc tgcttcatac ggtgggcgtg gaaaagggcg gcgatatccg catcggaacg
    34321 gacggcggca ggtttgcgaa ccctcttctt cattgcggaa agcacctgtt tggcattgtg
    34381 gaaatcgccc tgccagacag ttgcaatatt ttgataggca gccttcaaaa tgccgtttgc
    34441 accgctttcg cggacataat gccaaccttt gggcggcttt tgcaaacttt cgttgcgcca
    34501 ttcgaacccg ttatcgggaa aaataaaaga agacatggaa tacctgcgtc atgttttgaa
    34561 aatagggcgg cagaaccgca aaccatacgg atggtacagc aaggagcggc aacacagaac
    34621 agttttttgt tcccgccttg tctttccaag cccatgccgt ctgaagccgg aatgtttcag
    34681 acggcatcgc atcaaactcc ataaataaac cacatatgct tgaaataata ccttcaaccc
    34741 caatgtacgc gaaaatcggc aatctttcag acacaagaga gtacctatga cacaaaaaga
    34801 aaagcatttt gaggaatatg ccgccttggc aacccttcct ttgcgggatg tcgtcgttta
    34861 cccgcatatg gttctgccgc tgtttgtcgg caggccgaaa tccatcgccg cactggaaaa
    34921 cgccattacc cgcgaggagc cggttttcct gttggcgcaa accgatgcgg cgctagaaga
    34981 accggttgcc gccgacctgt atcagaccgg tacggtcgca caagtcctgc aagtgttgaa
    35041 actacccgac ggcacggtaa aagtattggt cgaagggctg tatcgcggac gtgttctgac
    35101 cattgaagac acgggcggtc tgttcgtttc ccatatagag acggtcgtgg aagaagacac
    35161 gggcggcaat accgacctcg aagccgtgcg ccgcaccctg ttggcgcagt ttgaacaata
    35221 cgccaaactc aataaaaaaa tccccgccga aattatcggc agcattaacg gcattgccga
    35281 aaacagccgg ctaaccgata cggtcgcagc gcatttgcag ttgaaactgg cgcaacgcca
    35341 acagattttg gagatttccg aaatcggcaa acggatggaa ttcctgctgg cacagctgga
    35401 atccgaactc gacattatgc aggccgaaaa acgcatacgc ggacgcgtca aacgccaaat
    35461 ggaaaaatcc cagcgcgaat attatctgaa cgaacagatt aaagcgatac acaaagaatt
    35521 gggcgaagaa gacgaaaacg gcgaactgga tgccttggaa gcaggcatca aaaaggcggg
    35581 gatgagcaaa gaagcggaag aaaaatgcct gtccgaactg aaaaaactca aaatgatgcc
    35641 gccgatgtct gccgaatcca ccgtcgtacg caactacatc gacaccctgc tcgaactgcc
    35701 gtggaagaaa aaatcccgcg tcagcaaaga catcgccaaa gccggattgg tgctggatac
    35761 cgaccactac ggcctggaaa aagtcaaaga acggattttg gaatacctcg ccgtccaaaa
    35821 acgtatggac aaactcaaag gcccgattct gtgcctggtc ggccctccag gcgtgggcaa
    35881 aacctctttg ggcgaatcca tcgccaaagc aacggggcgg aaatatgtcc gcatggcttt
    35941 gggcggcgtg cgcgacgaaa gcgaaatccg gggacaccgc cgcacctata tcggctccat
    36001 gcctggtaag attctgcaga atatggcaaa agccggcgta aaaaacccat tgttcctgct
    36061 cgacgaaatc gacaaattgg gtagcgactt ccggggcgat cccgccagcg cgttgctcga
    36121 agtgcttgat cccgagcaaa acaacaagtt cgccgatcat tatgccgaag tggattacga
    36181 tttgagcgat gtgatgttta ttgcgacttc aaacagcctg aatatcccga ccccgctgct
    36241 cgaccgtatg gaaatcatcc gtctgtccgg ctacaccgaa gacgaaaaaa tcaatatcgc
    36301 gatgcagtac ctcgtgccga agcaaatgaa gcgcaacggc gtgaaagaag gggaattggc
    36361 aatcgaagaa agcgcggtgc gcgatattat ccgttactac acccgcgagg cgggcgtgcg
    36421 ttcgctcgac cgcgagattg ccaaaatctg ccgcaaggtg gtgatgcaga ttaccttgga
    36481 cgaagataag aagaggttgt ctgaaaccaa gaaaaccagc aaagccaaac ctaaagcggt
    36541 taaagtaaat gagaaaaatc tgcacgacta tttgggtgtg cgccgcttcg attacggcgt
    36601 tgccgaaagc gaaaaccgta tcggacaagt aaccggtctg gcgtggacag aagtcggcgg
    36661 cgaactgctg accgtcgaag ccgcagcatt gccgggtaag ggcgtgattc agtgcaccgg
    36721 ccagttgggc gatgtgatga aggaatccgt gtctgcagcg tggtcggttg tccgctcccg
    36781 cgcggaatca gtgggcttgg ctcctgattt ttacgagaaa aaagacatcc atgtccatgt
    36841 tcccgaaggt gcgacgccga aagacggtcc gagtgcgggc attgccatga ctttggcgat
    36901 ggtgtctgca ttcaccaaaa tcccggtgcg cgccgatgtt gccatgacgg gcgaaattac
    36961 cctgcgcggc gaagtcctgc cgattggcgg tctgaaggaa aaactgttgg ctgcgcttcg
    37021 cggcggcatc aaacacgttt taattccaaa agacaacctc aaagacttgg aagaaatccc
    37081 tgaaaacgtg aaaaccggcc tgaccatcca tccggtcaaa tggatagacg aggtattggc
    37141 tctgggtttg gaaagccagc ctgagccttg ggcagaacct tctggtgcgg aagcggcggc
    37201 ggaatccgct tcaaaaccaa aaccccgcag cagggcaacc aaacattgaa acgcaggaaa
    37261 tgtgttgtaa aaatgcggtt tcgtcctgaa agcctgtcaa atagggtgat tccgtatttt
    37321 ttgcttgaca cggcaatttc agaattgcta taaagcgaaa gttgctcaag cagtacaaac
    37381 cccgagtatt cgggcatttc atcatttttt aagacaggaa gggactgatt gtgaacaagt
    37441 ctgaattgat cgaagcgatt gctcaagaag ccgacatttc caaagccgcc gcacaaaaag
    37501 ctttggatgc cactaccaat gcagtaacca ccgccctgaa acaaggcgac accgttactt
    37561 tggtcggttt cggtactttc tacgtgggcg aacgtgcgga acgccaaggc cgcaacccca
    37621 aaaccggcga gcctctgacc attgccgccg ccaaaacgcc taaattccgc gccggcaaag
    37681 ctttgaaaga cgcactgtaa gccgtttttt atgaaaaaag ccgattcttt aaagaatcgg
    37741 cttttttatc ggtccacatt attctgattt caaatcggca acacactgct tgtcacgtgc
    37801 ttcaaaggca tttgcgccgc cgagcaggtc aagctgttct tgtgcgccga gtttgccgaa
    37861 ggatctaatc tgtttctcgc tcaatctgtc caaaggctgc tcccacatac atttgcagta
    37921 gtcgacggcg aggcgggtat tgtttgaatc taaaccgcgg gcccgcaaat cgttttgcca
    37981 tttttcggca aacggaatat tcttcacgca agactcgaca attttctgtt ttgcctgcgg
    38041 tttggacatc gcgcattggg agagcagggc ggttaaagcg agcaacgcca aaatgaccca
    38101 cgcccaaatg cggatggtgc ggattttggc ttttgctttt ttgcgcgcgg caacctgctc
    38161 tttcgtcagc atttcgtgtt tcggctcagt catgcaggct ttccatgcgg atcatggtaa
    38221 tcggtttttc cacgcaatcc agtgcttcga tggctgcgat tgccgacttg atgtgttttt
    38281 cgaccgtgct gtgggtcaga atcacgattt cggcagtggt ctgatcaatc acgccttttt
    38341 gaatcagtgc ttcgatggac acgttttctt gtgccaacag cgcggcgatt tgccccagcg
    38401 tgcccggttc gtctttggct tggacgcgca ggtagtagct gctggtaatt tcgtccatag
    38461 gcaggatggt ttgcgcttgg acttgcgcgg gttggaacgc cagatgcggt acgcggtggg
    38521 cggtatcggc ttcaaccagg cgggcgatgt cgatgatatc ggcaaccacg gcggaagcgg
    38581 tcggcaatgc gcccgcgccc gcgccgtaat ataaggtttc gccaaccata tcggcgttga
    38641 cgcgcacggc gttcatcacg ccgttgacgt ttgccaagag gcggctttcg ggaatcaggg
    38701 tagggtggac gcgcagctcg atgcctttgc cggttttgcg ggtaatgccc aacagtttga
    38761 tgcgatagcc aagttcttcg gcgtatttga tgtcgcggct gtcgagtttg ctgatgcctt
    38821 cgaggtagca ggcggaaaag ttcatcggcg tgccgaatgc cagtgcgctc atgatggtga
    38881 ttttatggcc cgcatcgttg ccttcgatgt cgaaggtcgg atcggcttcg gcataaccca
    38941 atgcctgcgc ttctttcagt acatcggcaa acgcgctgcc tttttcgcgc atttcggaga
    39001 ggatgaagtt gctggtgccg ttaataatgc cggcgatgga tttaatcctg tttgctgcca
    39061 aaccttcgcg cagggctttg atgattggga taccgcccgc tactgcggct tcaaattgca
    39121 ccatgacatt tttttcttcc gccagcgaga agatttcgtt gccgtattcg gcgagcagtt
    39181 ttttgttggc ggtaacgatg tgtttgccgt tttcaatggc tttcaacacc gcatctttgg
    39241 caatgccggt accgccgaac aattcgacga cgacatcgac gtcttcacgc gcgaccagtt
    39301 cgaacgggtc tttgacaaag gctgcggacg ggcagatttg gcgggctttt tcttcgctca
    39361 agtcgcatac ggcactgatg cgcacttcgc gtcccaaacg gcggctgatt tccgccgcat
    39421 tgtcccgtaa cacggcagcc gtaccgccgc cgaccgtacc taaacctaaa agaccgatgt
    39481 ttactggctt cattgtgtct ccttgtaagc cgactgaaat gtaaatattg aaagacgaaa
    39541 atatccgctg ccgatataat tgtgccgcac tttgaatcaa atgccgtctg aaatcggcag
    39601 gcgggtcaga tgaaatctgc caatcctaca tgaattcgcc tgattttgca cccctttcgg
    39661 tgtagatgat gcggcgacgg ggtaaaaaaa tgttgtttga agaaaatccg atagacgaac
    39721 attttgcaga gtatgaccgc cgcacgggtg aaatccggct gtccggcaga catttcaata
    39781 agcctgtgct gctgcatagg gattctgccg gcctctcgaa atggcagtca ttgtcagaac
    39841 tgactccgga taatctgttg tctgatgtca aagcggatga ctatccggaa atattgatta
    39901 tcgggacggg cgcggctcag gagtttatcc atcccaaaat catggcggat ttttcccgaa
    39961 tcggaatcgg cgtggaatgc atgaataccg attcggcatt caggacattg gttttcctgc
    40021 actcggaagg gcgcagggct tgggcttggc ttcagccgta aatttcccat ttcagacggc
    40081 atcggcactg actttcaggt aaaatacggg ctttttccgc ccgacgatgt ttccgttatg
    40141 attgaaatca aaaacctcac cctgcaacgc ggtttgaaag tcctgctcga caaagccaac
    40201 gctaccgtca atcccggtca gcgcgtcggt ttgatcggca aaaacggaac gggcaaatcg
    40261 agcctgtttg ccttaatcaa gggtaaaatc actcaggacg gcggcgatgt ctcgattccg
    40321 aaaaactggc ggctcgcttc cgtttcccaa gaaacgcccg atttggatat ttccgctttg
    40381 gattacgttt tgcagggcga tgccgagttg caggcttttc agacggcatt gaggcaggcg
    40441 gaagcgcaaa atgacggcat gaagcaggcg gaatatcacg ccaaattgga agaaatcgac
    40501 gcttataccg cgccggcccg tgcagcgaaa ttgttgaacg ggctgggttt ttcgcaagaa
    40561 gaacacagcc gtcccgtcaa atccttttcc ggcggctggc gtatgcgcct caatctcgcc
    40621 caagccctga tttgccgcgc cgatttgctc ttgcttgacg aaccgaccaa ccacttggat
    40681 ttggaaaccg tcttgtggct ggaaaaccac cttgcctctt taccctgcac gcaaatcatc
    40741 atttcccacg accgcgattt cctcaatgcg gcaaccaccc aaaccatcga attgtcccag
    40801 caaaagctca cgcaatacgg cggcaattac gatttttacc aaaacgaacg cgcgcagcgt
    40861 ctcgcgcaac aacaagctgc ctatgtcaaa cagcaggcgc aaatcaaaca tctgcaatcc
    40921 tttatcgacc gcttcaaagc caaagccacc aaagccgttc aagcgcaaag ccgcatgaag
    40981 gctttggcaa agctagaacg cgttgcgccc gcgcatctgg acagcgagtt ttcctttgag
    41041 ttttaccatc ccgaccatct gcccaatcct ttgttaaagc tagaacacgc tgatttgggt
    41101 tacgaaggca aaaccgttct gcatgacatt accctgtcgc tggaaagcgg cgcacgctac
    41161 gggctattgg gtgtcaacgg cagcggtaaa tctaccttta tcaaagcctt ggcgggcaaa
    41221 atcgatttgc tttccggcag catcgtccgt tccgaaaaac tcaatatcgg ctattttgcc
    41281 caacaccaac tcgataccat ccgcgccgac caaaaccctg tttggcatat ccagcagctt
    41341 tctcccgaag tacgcgaaca agaaatccga aacttcctcg gcggcttcaa ctttgccggc
    41401 gatatggcgt tgcagaaaac cgaacctttt tccggcggag aaaaagcccg tctcgccctt
    41461 gccatgatta tctggcaaaa gccgaacctg ctgctgcttg acgaaccaac caaccatctg
    41521 gacttggata tgcgccacgc cctgacgctc gctctgcaaa gtttccaagg cgccttaatc
    41581 atcgtatcgc acgaccgcag cctgcttgaa gcgactaccg acagcttcct cctgatcgac
    41641 aaaggccgtc tgaagaactt cgacggcgat ttgaacgatt accgccaatg gcgtttggca
    41701 caggaaaacg ccgccgccgc acccgcagca tccgcacaaa gccaaagccg caaagacacc
    41761 aagcgcatcg aagcgcaaat ccgtcaggaa aaagcccgac gcggcaagcc gatacagcag
    41821 aaaatagacc gtgccgaaaa agaaatggcg cagctttccg aaattcagac ggcatgtgaa
    41881 gcatttttag cacaagaaga cgcttacctt gaagcaaaca aagaaaaatt gcaaaatacc
    41941 ttatccctgc tggcaaaagt caaaacacaa cttgcccaaa tcgaagaggt ttggctggct
    42001 tgtcaagaag aattggaacg gattgaaact gaaatcgaga aacagtttgc cgagcgataa
    42061 agaagcgcgg acagggtgct tatctttgcc tgccaataac ggtataattc gggcgttatc
    42121 accgcctttt accggtataa acatcagact ttttgccgcc ttgggtctcc tgtccctttc
    42181 cggcgcggcg gcgcaagcct ccgtatacca ttgcaactca aatggcaaaa gcgtatatac
    42241 atcagcccca gcaggatcgt gcgccgatgc ggatttacct aaaatcagca gccatcaggg
    42301 aggcggatac cgcctgaaaa ttaaaaaact cagttaagaa gccaaaatac acatagaaag
    42361 taaaaagaaa aacaaaaaac ctgccgggaa aaagaacaag caggctgcca aagccccgaa
    42421 agaaaatcaa aagcaaccaa ccgaaaagaa aagcccgtaa aacgccaaga aagcattaca
    42481 aaaaatacca aaaaatcaaa taattatccg agcatcaagc acattatgaa atcaaaactc
    42541 cccttaatcc taatcaactt ttccctgatt tcaagcccat tgggtgcgaa tgcggccaaa
    42601 atctacacct gcacaatcaa cggagaaacc gtttacacca ccaagccgtc caaaagctgc
    42661 ctctcaaccg atttgccccc aatcggcaac tacagcagcg aacgctatat cccgccccaa
    42721 acatccgaac cgacaccatc accgtcaaac ggcggacagg ctgtcaaata taaagccccg
    42781 gtcaaaacag tatccaagcc ggcaaaatcc aatacgcccc cgcctcaaca agcacctgta
    42841 aacaacagca gacgctccat tctcgaagca gaattgagca acgaacgcaa agccctgact
    42901 gaagcccaaa aaatgttatc acaagcacgt ctggcaaagg gcggcaacat caaccatcaa
    42961 gaaataaatg cattacaaag caatgtattg gacaggcagc aaaatatcca agcactgcaa
    43021 agagaattgg gacgtatgta aagccgtgtt ttcaaatcga ccgttccaag gatttgacag
    43081 aagaaatgat gaaaaagcag cggcatatcc tgtgtgtcgg ctgcctgtat attatattga
    43141 aggtcaacgt atttcccaat accggctgga tggaagcatc gaaagggatg cagggcatgt
    43201 cgttcagtac cttcggatac gcggatgctc cgggctatac attcagccgc attggccata
    43261 ctttaatttt cctacttgga aagaccacag ggacacgtac aactgtttcc gatagttcgc
    43321 ataatgtata ttatgttaaa ttatatattt gtgcaaataa ctccaaggca ttaagctgtt
    43381 gtttgatgct ttgccagttt gggagaaagt tgtccatatg cgccataaaa cgggcgttgc
    43441 gatggcgttc gagcaaatgg gtcagttcgt ggacgacgac gtattaagcg gtgcggacac
    43501 ccgaaccgcg atcccctaaa tgtcttggtg ggaatttagg ggattttggg gaattttgca
    43561 aaggtctcaa aacccgctcg ccttatagcc tgtctgtgcc gccatttcag cctccaaaac
    43621 ccatattttc aaggtgggca ttgactttgc tttgccactt ttccacttct cgggcaagct
    43681 cggccatcgg gctgcggtag cggtcttcca gcgtgttcaa gcggctgata agctgctgaa
    43741 tactgttttc caagcgattt tcgattcggc tttgtaaatc ggcaagccat ttgtcttgaa
    43801 cggcaagctg cttgatttcg gcttcggaaa gtcggccgaa ttgtttgaat acggcaaggt
    43861 tcagggcttc gatttgggtt ttgactgcgt ctttcgcggc tttttcctgc gtcatcagtg
    43921 tttgggtggt ttgtaaaaca gcccgttcgc cttcttctat gccgctttct tccaatgcgg
    43981 ttttcagaag tttggcggaa agtttgcctt ttgcatccaa tacgtcgttc agcgcaccct
    44041 cttcgccgcc gtgttcttct atgtggtttt ccaattcttg gcttaggcgt tccagctcgc
    44101 tttgtttttc ttccagcttg gcgatgccgt ctgaaaagta gcggcgggca accagctcgg
    44161 gggcgatgac ttcgctgcgg tattttttgc tgatgcgctt ggttttggct ttgccttttt
    44221 tgccggtttc ggtttcctca aagacgacgg tcaggttcgc ggcttcatcg ctttctttgg
    44281 tgatttcggc caggttttta accgccttcc agccatcttg ggcgatgaga taaacatcgt
    44341 cttgcagggt ttccgcccag tagtccgtca ggatttggta gaaatcgtat tcttcaatca
    44401 ggctgccggg tttgaacgcg tccagcaggc tttcgctcca tttccggata agcctgcccg
    44461 gccggctgcc cggccggctg cccggttgga tgacggcaag gtcgttttga gtgtgccacg
    44521 cggcaaactt tgctaggtgt ccggctttga aggcggcgta atcggggtgc gccaatatat
    44581 gggctttgat ttggctgctt tcgattttat agtggattaa atttggggct gtactagatt
    44641 agccctaaat tccacaccaa tcccgcagga ttttaagctg ttgagacggt gtgccgaagt
    44701 taaatcgaaa ttcgcattct ttcaagaaca gtgggaaaga tttgcaatcg attccgttgt
    44761 atagtggtaa agtggccatc gtgttcggca aacaactcgt ttttcatacg gcctaaaact
    44821 tgccaatagg ctttcaatgc gtccatatcg tgcgcaggta tgccgccatc cataaagata
    44881 ggcggcgaga ttttacaggt cttcgacttc tccgctgtcg atatagcgag gcagattaag
    44941 gttgtaatct tgtgctgcga tttcgcttaa atgcaccata cggctgtaac ggggaatttc
    45001 ttcctgtttt gtgaaggtgt cgatgatttt gtgaatgtct tgttcgcgca gacggttttt
    45061 gttgccgtct ttaataaagc cgcgcgatgc gtcaatcata aacacgctgc cgccactgat
    45121 aacttggttt gttccctctt cggcaaattg ggcggtttgg gcgtgttctt tgtcgatgac
    45181 gatgatgcag gcaggaatgc ccgtgccgta aaacaggttg gcaggcagcc cgataatgcc
    45241 tttaataagg tcaaggttaa gcaattccgt gcgaatacgc gcttcggcat tgccgcgaaa
    45301 cagcacaccg tgcggaagaa taatcgcacc tttgccgctt ggtttcaggc ttttgagcag
    45361 atgcagcaaa aaggcgtaat cgccgttttt ttcgggcggg ataaggcgga ttggcaacgg
    45421 caaaatcgaa ggtcttaagc ccgtcgtttt catcacggaa agacgaatcg gacaaggtgt
    45481 tcccggtgtt gatttcggcg gtttcgttgt tgtgcaaaat catattcata cgggcaaggg
    45541 acgcggttgc cacatctttt tcctgaccgt aaaggctgat ttggctgccg gcttgggcgg
    45601 cggctttgag caacagcgaa cccgagccgc aggtcgggtc ataaacgctg gtgctggacg
    45661 gcaatctgcg ctgattccga taatcttcgc cataatgcgg gagacttcgg caggcgtgta
    45721 aaactgccct ttggatttgc ctgactcggt cgcaaaatgg cgcatcaggt attcgtaggc
    45781 atcacctaac aaatcgtcgt cttcggcttg gttggaagaa aggttgagct ttttaaaaat
    45841 gccgacaagc ctgctcaaac ggtcgatcat ctccttacct ttacccagtt tgtcttcgtc
    45901 gttgaagtcg gtaacgttga tcacgccttt taagtcgttg gcttcggcaa tttgggcgat
    45961 aatcttattc aggcggtcgc cgatgtcggc ggtgtttttg agtttgacga tgtcgtcaaa
    46021 agtcgtaccg gcgtgcagct caatcatgcc gccgccgtac ttatgcttat cagaaacata
    46081 ttttaaaaac aatagcgtaa gcacatagtt ctttataaag gctcgcatcc ataccgccgc
    46141 gcaactcgtc gcagcccgcc caaagtgatg cataaagctg attttttttt gattgccata
    46201 aggttttgcc taataaatgg aaattacaaa tcaaacgcga ttttaacacg ccggcggcaa
    46261 cggctatatc ggcattcaca gcttccagtt tatcggatcg ataccgtgcc ggctcaaata
    46321 gctgtttgcc tgtgaaaaat ggcggcaacc gaaaaaaccg cgatatgccg acagaggaga
    46381 cggatgcggt gcggtcagta tcaaatgatt ttgactgtct atcagtttcc ttttttgttg
    46441 cgcataccca ccccacaaca tgaagacaag gtgcttgcgg tgcgccgcaa gctgcctgat
    46501 gacggtatcg gtaaagcgtt cccagcctaa aagggcgtgc gaatgcgcct gtcctgcacg
    46561 caccgtcaaa accgtgttca gaagcaatac gccctgctcc gcccacgctg tcagacagcc
    46621 gtgcgcggga atggaaaagc cttcgatgtc ggtttccaac tccttgtaga tattgagtaa
    46681 agacggcggt atgcggatac cctgccggac ggaaaatgcc aaaccgtgcg cctgccctgc
    46741 cccgtgatac ggatcttgtc cgagaatgac ggctttgacc cggtcgaacg ctgtcaggcg
    46801 gaatgcgttg aacacatccg ccgccggcgg atagatgatt tgtcccgaca aacgttcctg
    46861 cctgactgca tttaaaattt cctgaaaata cggctgctgt ttttcgccgc cgagtgcatc
    46921 gtgccaagtg tccatatctg ccctatcctt tcaaattcgg ctatactgct gatattgcgt
    46981 tattttgtca aacgacgcgc tttgtgggga aatgtatgac tgtatggttt gttgccgctg
    47041 ttgccgtctt aatcatcgaa ttattgacgg gaacggttta tcttttggtt gtcagcgcgg
    47101 ctttggcggg ttcgggcatt gcttacgggc tgaccggcag cacgcctgcc gccgtcttga
    47161 ccgccgctct gctttccgcg ctgggtattt ggttcgtaca cgccaaaacc gccgtgggaa
    47221 aagttgaaac ggattcatat caggatttgg atgccgggca atatgccgaa atcctccggc
    47281 acgtaggcgg caaccgttac gaagtttttt atcgcggtac gcactggcag gcgcaaaata
    47341 cggggcaaga agagcttgaa ccgggaacgc gcgccctcat tgtccgcaag gaaggcaacc
    47401 ttcttattat cacacaccct taacactcgg aggaattatg gaatttttca ttatcttgtt
    47461 ggtagccgtc gccgttttcg gtttcaaatc ctttgttgtc atcccacaac aggaagtcca
    47521 cgttgtcgaa aggctcgggc gtttccatcg cgccctgacg gccggtttga atattttgat
    47581 tccctttatc gaccgcgtcg cctaccgcca ttcgctgaaa gaaatccctt tagacgtacc
    47641 cagccaagtc tgcatcacgc gcgacaatac gcagctgact gttgacggta tcatctattt
    47701 ccaagtaacc gaccccaaac tcgcctcata cggttcgagc aactacatta tggcgattac
    47761 ccagcttgcc caaacgacgc tgcgttccgt tatcggacgt atggagttgg acaaaacgtt
    47821 tgaagaacgc gacgaaatca acagtactgt tgttgcggct ttggacgagg cggccggggc
    47881 ttggggtgtg aaggttttgc gttatgagat taaagacttg gtgccgccgc aagaaatcct
    47941 tcgctcaatg caggcgcaaa ttactgccga acgcgaaaaa cgcgcccgta tcgccgaatc
    48001 cgaaggtcgt aaaatcgaac aaatcaacct tgccagtggt cagcgcgaag ccgaaatcca
    48061 acaatccgaa ggcgaggctc aggctgcggt caatgcgtca aatgccgaaa aaatcgcccg
    48121 catcaaccgc gccaaaggtg aagcggaatc cttgcgcctt gttgccgaag ccaatgccga
    48181 agccatccgt caaattgccg ccgcccttca aacccaaggc ggtgcggatg cggtcaatct
    48241 gaagattgcg gaacaatacg tcgccgcgtt caacaatctt gccaaagaaa gcaatacgct
    48301 gattatgccc gccaatgttg ccgacatcgg cagcctgatt tctgccggta tgaaaattat
    48361 cgacagcagc aaaaccgcca aataagccgt gatgaaatgc cgtctgaagc tctgttttcg
    48421 gggttcagac ggcattttta tttaaactcg ggcagggttg tattccgctt caaatatagt
    48481 ggattaacaa aaaccagtac ggcgttgtcc tatttgtact gtctgcggct tcgtcgcctt
    48541 gtcctgattt ttgttaatcc actatatgtt tcggaaccgc ctacctgccc cggtaaaaac
    48601 tgccggcaaa atatttctgc acgccctcgc gaagcaagac caaggttacg gcggccaaag
    48661 gcaatcctgc caacattccg acgaaaccca tcagctgtcc gaacgccatc agggaaaaga
    48721 taacccaaaa cggcgacagc ccgatacggt ctcccacgat tttcggcgta atgaaaaaac
    48781 tttcgagaaa ctgtcctacg gcaaaaaccg cccaaaccga taggatgccg ttccacgaac
    48841 cgaactggag caaggcggcg acggttgcca gcagcagtcc tgtaaacgcg cccaaatagg
    48901 gaacaaaaac caaaataccg gcaaccatac cgattgcaaa ccccgaatcc agcccgacca
    48961 gcaccaaccc caagccgtaa accaaaccca taatcagcat caccagaagc tgcccgcgca
    49021 aaaattcgcc caatacctcg ttcaaattac ccgtaatgcg cgtataagca ccggcaaaac
    49081 gcctcggaac cagtttggcg atgccgcacg accaccgctg ccaatccagc aggaaataat
    49141 aaagcagcaa gggcagcagc agtaggttgc cgatactgct gacaatattg ccgccctgcc
    49201 tcatcaaaac gggaaaccac gccttaagcg cgttgctcaa ctcgcccgta tgcgcctgaa
    49261 gccacgcaat aatagatgcc tgatcgattt ccatatatcc gccgattgta tttttcaacc
    49321 acggcagcag cgtgttctgc ataaaaccga ttaattgggg caggcgcgat gccaaattgt
    49381 tgaactgccc gaccagcata gggacgataa tcaacaataa tgccaacaac aaaatcaagg
    49441 aaaacaccat cacagacatc gaagcggatg cacggttcaa accctttttc tgcaaccatt
    49501 cgaccaaagg gtccaataca tacgccagca ccgccgcaac cgcaaacgga gtcaaagtat
    49561 cgccgagcgc gaaaaccagc caaaccaagg cggcaaacgc cgcaccggca cccatccacg
    49621 gcttgatgcc ccgccctttc ctccgataca taaaaaccac ccttctaaaa ataatattgc
    49681 gcgccagtat agcagaaccg ccgcgccgtc aaaaaagcct ccgccttccc gatagcctgt
    49741 tatcgcggtt taggctaaaa taacgcacat ccgatgccgt ctgaaaggca aacccgcctt
    49801 cagacggcat accgtacaca acaaggcagc acatcatgac cgaacaaaaa aacaaaacat
    49861 ccctcatcga atttccctgc accttcccat tgaaagtaat gggcgcggtg catcccgagt
    49921 tcgagcaggc ggtattggac accgtccgcc ttcacgcccc cgacacgcag gcgcaccaca
    49981 tcaccacgcg tccgagcagc aaaggcaact atactggcgc caccgtacag gtaaaggttg
    50041 aaaaccaaga acaattggac aacatctacc gtgcgctgac ttcgcacgaa ctggtcaaag
    50101 tggtacttta gagatgaaaa tcatacacaa aggtttggtc gaatatctgc cgacttttga
    50161 agcgatgaaa acctttaatg ccggacggaa tgccgacacc gaagacgaac tgtgggtcgt
    50221 cgaacacccg cccgtgttca cacaaggact ggcgggaaaa cccgaacacc tgctgattcg
    50281 cgacgacatt cccgtcgtcc aaatcgaccg gggcgggcag attacctatc acgggcccgg
    50341 gcaattggtc gtttatacga tgattgattt caaacggcgc aaaaccagtg ttagaaacat
    50401 cgtttccgcg cttgaaaaca gcatcatcgc cacattggca gaatacggca tcgaagcggc
    50461 ggcagacccc aaacgccccg gcgtttatgt cggagaacgc aaaatcgcct cactggggct
    50521 gcgtatcaaa aacggctccg tctatcacgg gcttgcgctc aacgtcaata tggatttaag
    50581 cccgtttacc cacatcaacc cctgcggcta cgccggtatg gaaatgacgc aaatcgcgga
    50641 ttttgtccaa ccctgcccca cgccggacga agtcgccgcc aaactcaccg cacaccttga
    50701 aacacaattc acaccgaaag cagacaacaa tgagtgaaat caaaaccgac gaccccaaac
    50761 gcggcatcaa actcagaggc gcggacaaaa ccgcgcgcat ccccatcaaa gtcgtccccc
    50821 ttcaggaaaa actgaaaaag cccgaatgga tacgcgccaa actcccatcg cgcaaattct
    50881 ttgaaatcaa agacattttg cgcgaacaaa agatgcacac cgtttgcgag gaagcctcct
    50941 gccccaacat cggcgaatgc ttcagcaaag gcacggcgac cttcatgatt atgggtgaca
    51001 tctgcacccg ccgctgcccg ttctgcgacg tgggacacgg tcggcccaat atgctcgacc
    51061 ccgacgaacc gagaaacctc gccgaatccg tcaaagccat gaacctgcgt tacgtcgtca
    51121 tcacctccgt cgaccgcgac gacctgcgcg acggcggcgc acagcatttc gccgactgca
    51181 tcaaagccat ccgcgaaacc agcccgaaca ccaaaatcga aatcctcgtc cccgacttcc
    51241 gaggacgctt ggacatcgca ctcaaaatcc ttgccgaaac cccgcccgac gtgatgaacc
    51301 acaacctaga aacccatccg agcctgtaca gaaaagcccg tcccggtgcc aattatcaac
    51361 attctttaga cttattaaaa cgttataaag aaatgatgcc gcacatcccg accaaatccg
    51421 gcatcatggt cggcttgggc gaaacagacg aagacgtgcg tgaaattatg cgcgatatgc
    51481 gggcgcacaa tatcgaaatg attaccatcg gacagtacct ccagccttca gacggacacc
    51541 tgcccgtcct gcgctacgtt acgcccgagc agttcaaaat ctttgaaaaa gaagcatacg
    51601 aactgggctt cagcaatgcc gccatcggcg cgatggtacg ttccagctac cacgccgacg
    51661 aacaggctgc cgaagcctta cgcgaatgtg aattctgacg ctatttgaga ctgaactacg
    51721 ctatttgcga ctttataacg ctaagttcga ctgggcaaat atggatcaac aaccagttat
    51781 tgaaattgcc gaattcccac atgataataa attgtggcga attgatgggt tagggaagat
    51841 tttttcccaa ttgaaaaata acaataacct gtttattgaa gtctatttac gcccagttaa
    51901 ctttcacgat aattctgtat ataactttgc taaatttgat agttctggta gagaaattcc
    51961 agaatataat caattgaaaa agattcctgt taatataggc ttgcttccta ttttaagagt
    52021 gggatctctt tggataaaag gaaaacttca aaacaataat gcagttacag atatagccac
    52081 tcttaaaaat attgaaataa atgcaaatac agtagaaata attaatagca attacaaaat
    52141 aaaatattta gattcaagcg gaagagaaca agagtgcaaa ctattgttgc agaaaaattt
    52201 tgcaacggat gcaaaagtac ctttttcttg cttcaaaatt caaaatgaaa attacaaaaa
    52261 tggaataatt attccagtaa tagaaattat ccgtttttac tatgcctgtt caactagact
    52321 agctcattta acattttcta atccagacga aattacaaaa atctcatatg cgatagataa
    52381 tgaaacagac aaggtagtca tcaaattacc acagcaatat acagatcaag aagcctttat
    52441 tttagcaaga atattgactt caaatattgc tttatctgga ctctttcaga ttaaaaattc
    52501 cctaataaaa ataggagtta atgaacaacc aactacatta ttatctacca atttcccatt
    52561 tatcaatcca accaatttaa tggttcgcgg tattaaaatt gttgatagat tcttagttac
    52621 ggagatttgt tcttgttcgg ctccttttcc cgatatttct gttgatagga ataatgacaa
    52681 tcgaaaggga gataaaaaaa acagatatac ctgatgataa aaagaaagaa tatcgaagag
    52741 ggggacaaac tcctagtctc aagggaaatt ccactattca aaatcaatat gaatcagtca
    52801 attgcgtatc aacattacaa tttaacttaa acataaattg cttttcagat ttgatcggta
    52861 aaaagattga aaaaccccaa aagcccttta atgaatacaa agcacagaaa cttgccataa
    52921 atgaaaaaat taatgtaact agcttaggag tggatttagg tacatggagc caaactacaa
    52981 ttgtgcctgc gtccattagc acttttaata cccaatctga aaaacaaaat agggatgcct
    53041 tagctgcgac ttttgacaat tccgtacgtg ctattgaaca tttgaatgaa gaatattcag
    53101 atattcaggc aactatctac atgtctagct ctcgagcttg tgagtttgtt ccattaactg
    53161 actcagccca taaagcccaa tggtcgtatc ttgtttccaa aaccaaaaca aggcgtaagg
    53221 tattattagg aaaaattgag tacaaagata aaacattttg gttgattgaa attcaagctc
    53281 ggaaaagtga gcattttgtt acggcgatta tttattttga ggcaattgac gagacaaagt
    53341 atttgactca attattatat gtcttagcaa aacagcgggg aatttgggat aaagttccaa
    53401 aaaactccag tttgcaaatt attactctaa agcatacgca taaagatatc aagagctacg
    53461 ctcaaaaaat acacaaaaaa atatcaaatt ttatcttaaa ataaattagt tagtaatata
    53521 actaatctag ttataaatta ccaaaatact tcaggctgat acttttatat aaaatttaag
    53581 ttacctactc tcttattgat aaggctggtg gtgtcgtaac tactatatag gagtaatggt
    53641 tcccatctgc taacataaat gttaaggtaa gagtaaattc taaagacaca aaagaaaacg
    53701 gttcttttga tatttcaagg ttatgttttt aatctataac ataatattaa caatttctat
    53761 ttttaggtat tcaaaatgaa aaatcgagat actccgcata aaaaatttat caaccctacg
    53821 gaatatagtc tatttgcaga agaagaacaa tatgattttg aattaatgat tgaaaatcag
    53881 ataaaagaac atgaaaaaga aattgattac tatgtaactg attatcccat agaaacacta
    53941 gttgctaaat tagatcgaca agaattgttt attccagact accagagaaa aaacatttgg
    54001 gaaaaacatc gtaaaagtag gttcattgag tctgttttaa ttggcttacc tatcccattt
    54061 ttattttttt ggcaggatcc ttcaactgga aatatggaag tggtagatgg tgcacaacga
    54121 ctaacagcat tgtctgaatt tattcatggc aatttaaaat tatcctattt ggaaaagctt
    54181 atttatctta atggccaaac ttatcaccaa ctcctagaaa gtcgtcaaaa gattttcaaa
    54241 aataaaccta tccgaggcgt agtacttagt aataaaacag acaaagaagc tcgacttgac
    54301 ttatttgaac gtcttaatac aggtagcaaa aatgctacac ctgcagaaat tcgtcgaggt
    54361 ctgcttcaag gagcatttta cgatatggtt gagactttat ctgaagataa aacatttaat
    54421 gaattagcaa aaattagcaa gaatatgcag gatatggggg agcgagaaga attcattact
    54481 cgcttttttg cctatggtga gtatttagac cattattctg ctaatgttcg taaatttatc
    54541 tttgattata cagaaatcat gaatgaagaa tttgctaaaa atccaaacat gcgtgcgact
    54601 tacgaacaaa aattccatga aattatggct tttttcaaag aaaaatatcc aattggtttt
    54661 aaaagagatg gaaagggtca gtcttctcgt actcgtttcg aaggcatggc tgtttcagct
    54721 tatctcgctg aaaaggaaaa tccaggcatt ttagctcaag ttaccccaaa ggagtgcgtt
    54781 gatatttttg acagtaaaga attcattgaa gtaactggta gtgatggtgc aaatgccact
    54841 aaaaagctac atgctagatt attttttgct aaaaatgcct ttaaacggaa tcatccatga
    54901 actcagtaaa atctatgatg gaaagtcgtg aagaggaaat aaacctatat atggatctgc
    54961 taaagtatat ccatgatagt actcaacatg gtttaccagc tttacaaaat agaaatggtg
    55021 ttgaatatca attggaaaat actcatctcc atatccttac cgctagtatc tatatgttac
    55081 tatataacta cattgaggct gttgttaccc aaagcctaaa tactttaatt tgctatattc
    55141 aaaatgaaag gatgcctatt acccaatttt cagaacccat ccaaaaagaa tggctaatag
    55201 gagggttaaa ccttaatgat gcaaaagcta gtgtagaaac acgattaggt aatggactca
    55261 aaatgcttaa acatttcggt gatacacaaa aaatagatat taagatttct aaaggaggag
    55321 gaaattttaa tgatgacgaa atatttaagc ttgcaaagaa acttcaagtt aatatgtgta
    55381 tcgatgacga aactcaccgc caattacggc aagcaggaaa tattacctat gaaagtacgc
    55441 gcaaatttca agatacttgt atgaaagaaa taacccgtga acgaaataaa cttgctcatg
    55501 gagaaatcac ctttcaagaa tgtggtagaa accgaacaat atctgattta atggcaataa
    55561 aagacactac tttccgctat atgcatttat ttactgatgc atttactgaa tatctaatgc
    55621 agaagaaata tctcaaacca acagtttaaa actatttccg attgcctcac ctagcaatac
    55681 aggaacagca ttaccaatca ttcgactaat ttggcgaatt ggagtatcag tttcttgagt
    55741 gaactggtaa tctagaggaa aactttggaa taaggctgcc tcccttaaag aaattgcacg
    55801 gtcttgttcc ggatgcccaa agcgtccatt gccgaagcca atacataaag ttgtcattgt
    55861 tggggcgggc ttgtcccatt ccatacgacc ataaattcgt ttataagact gtcctttttt
    55921 tttgcgatga catgcggctc gtaaatcttc tggccaatcc atccatgttc ctcctggacg
    55981 agaagcacga atccgttcta gattacgagc tgacattgat gaacaccgat gtaatacatc
    56041 tatagtatct tgtttgcctg ctgccagtgg cggcaagtgt ccaatagcct gacgaactgt
    56101 tacatggcaa tttgaatgcg ttggaggaac catctgaata ttaccgatac gtgatgcaag
    56161 taatacgtgt ctacgtcgtg attgagggac tccataatct gcacataata ctttttcagc
    56221 ccaaacttgg tatccctgtt gttttaaatt atttacaaaa tcttggtata cctcatgttt
    56281 tacaacatct ggcacattct ccatagtcac aagctctggt tgcacttcat gaatcaagcg
    56341 agaaaaagcg tatagtaatg gccatttttt gtcattacga acatcacgac cttgattgta
    56401 agtagaaaat ggctgacaag gagcacaacc agctaataat ctaactgcat cttttgaata
    56461 gtgcgcctga atctgatctc cagtaactgt ttccacatct tgtaatataa attttgcacc
    56521 tttattgtta tactcgtacg catatgcaca agctgcatca atatcatatc cagctttcac
    56581 tttaattcct gctcgaagta aacctgctgt caacccacct gctccacaaa ataaatctac
    56641 tgcttcaatt ttcatttttc aactcatatt tttgatataa ggattatatc cttaactttg
    56701 attggagtcg agcttttaat agtatataag ctctgaatag tacagttcct tatgaacgtt
    56761 tctattcatt tttaaaatta tttcctgaaa actttggggg caatcattaa aatccttcaa
    56821 taaatggtta taacggacgg ctttatcttg ccatgcttta aatagatttt ttggaaaatt
    56881 ttgtattaac cacaaacaag catgaatagt actgtgtcgt tccgccaaac tgcgactttc
    56941 ccaactcagt cgacgagaac gttctaattg aactggaatc tgatgtattt gcttgcacaa
    57001 ataagagtac aaatccggtg caagcttctt tgatgtagta atcgccaaaa agtgatgcag
    57061 aatttttagt tgttcttttt cagattggtt aatcagtagc tagacatagc gggcaataaa
    57121 caatagcatt attatcgcgt ttacggtgat tggctttaag gctgtttatc cacgtcaatt
    57181 gtcctgaaaa attgttttta tcaaataatc gaccttgtag gctatctaat gtggttgttt
    57241 tgattcgaat ggttgaacag cctgtttttt cagccaatgt attgaccaac cagtcaggag
    57301 tatggcgatc tatatcccta ttccatatct cataattagg aaatagcaaa tgacagaagg
    57361 tttgtacttt caacccgttt gcatgtgcta agcgaaccaa ccatgatgta aacaattcat
    57421 caggattcgg aatttgtaat tcatttccta agtatttaat tacatttaaa aacgttttct
    57481 gcttattcaa actcccggaa aggatatggt ggatttcatc aataatcaac attttagtat
    57541 taacgtattt gagcaaatga agcacttggg caagtttttt atctgtcctg tcatgagttt
    57601 tatagggggc aaacagtaaa tctaaaatag cattgtaaaa acggctttca tcaggtatag
    57661 gcggtgcttg aataatcact actggtgcga taataccatt tccatgtgga ttatcatggg
    57721 ctggatgttg ccgcaaaaaa tgtttaatca acatggtttt accgttatta gtatcaccaa
    57781 ccaacaacat attaggcata cggttttgtt tcgggaaact tagcaaatct tccagtttta
    57841 ccaaaacctg ttgagcctgc ggataaccta tccaacgagg ggaacgtatg tattcgatgc
    57901 gctcaatatc gctatacttt agttgttctt ttgctttggg agaaaggtga ggataattca
    57961 tgataaatcg tccaaatcat caaaaggctg aatatcatca aacatatttt cctcattttc
    58021 agacgatata atattcctcg aatcaatttg agacataggt aaagatgctc gagtacgttg
    58081 atctaattgg cgacgttgtt gagcgcgacg gatagcttta gtcttacctt gagcttgctg
    58141 ttccaattct cgcattcgtc cgtatgctgt aaaaatcatg ttctcatcaa tattctgttt
    58201 ctgctcattt tttaattgtc tctgaatctc tcgtaactcc caaatgctga taactggatt
    58261 cgaactgttc cgataaggta ttggaaaata catcgataaa tctgggtcat aaaaccaaat
    58321 cacgcttaaa tcacgtggat ctatgcgaaa aatgaactgg cgagcctgtc taggattagt
    58381 cggattagtt gctgcaatcc atggtcgtaa aacgtcatgc caataagtga ttttatggat
    58441 aactacccca tattgctgca ccgttcgcat ttcaaacggc ataaaggtca agcgcaatag
    58501 tgtctcatca accactttat caggtaatcc tgttcctaaa gaaacatcat ctcctaatac
    58561 acctgatttc catttggcta acggtgagat ccccaatcca gaatgagtac gctgatgata
    58621 aacatcacaa ataaatagtg ttaaccacgt ttcaaattca gttagcgtta atgccgcctt
    58681 gctttcggaa gcataatctt ttctctgttg tgtatttgca aaagtagtac cagttaaacc
    58741 gtggacttca tccaaaatcg tacctagtaa acgctcaatg tgtcctccga aatgaggacg
    58801 tgccacaggc ctccactcaa tttcaatacc gtattgttta cacgcctttt ccagcacttt
    58861 gccacggaat tcttttgcat tatccaaatg aattttgcgt ggcaaacccc acacaggcca
    58921 ccgccctttc actccatgtt ttgccaacca gctttcctta gacaaaatac tatgtgctaa
    58981 acacaaacct gtagataatg cacttggtgg atcgaatgac acataaaatc ccgtaaccat
    59041 tcgactgaac acatcaatcg ccagcgtaat ccacggccta ccaataggct tacgatatat
    59101 atcgtcaact aaaataatgt ctaactttgt atgatctatt tgtaccactg aaagcggata
    59161 atcggcattt ggaaagctac cttgtaaagg agaaaatttc atttctgctg ctttatggct
    59221 tagtcgttta gcaattttaa tttgaggcgc aatatttttg attcgagaac gaatggtatt
    59281 agaaagataa tcaagattag caatttggtt taaccaattt tgccattgca gcctgatttc
    59341 atcattaaag aaaataacat tctcacaagg actgttgctc aagtcatatt gacaattcca
    59401 acaatatttc aactctgtaa agcgctgcaa attaggttta cctaaatctt gtcggtgaaa
    59461 agcaatatat atgccacatt taggacagca atcatgcatc atgatttgat gtattggaca
    59521 aaatgtatat agggctatgc gccatgattt tcggaaatat gtttcatggc atgaggagct
    59581 ggtaaatttt gtttatggca acgccgtgta atttctctaa tgattttctg tattgattta
    59641 cgttgtgtgc ttaaataatc cacttccaat acttgcctga taagctgttc tacatcatca
    59701 gaaagctgtg ttttaccact atcactacgc tgcttacgtg ttaaagcagt cagaacgccg
    59761 ctttccttat aactttttaa ccagcgataa accgtactga tatgtacacc gcattcggca
    59821 gctctcgctt gaacatcaga tttactacgt tttggatttc ggataagtgg ctcaattaca
    59881 tcgaaatgtt gttgagctaa ttgccatttt tgctcaggaa tactatctaa gtgggttaat
    59941 tcagcactct ggactgttga tagatccgct aattttgctt ggtaatctgc accggttaca
    60001 ctatcgcgta actgatatgt ttctaactcc aggactgctg taattacaca gattttgttt
    60061 tgaaacaaaa ccattgcacc gacattcaca tttaaagttg ctttcatttt ttaaaccata
    60121 tagcggattg catagtgagt ggttgattta aatctgcgcc aatttttcgt tctgcaatta
    60181 agtaccacaa tacaggcaat aattttgctt gattgtattc atcctgaaaa gtctgttgaa
    60241 taagctgttt aggtgtggat tttttcaatt cgcgtaacga ttcatccaat aaagtcataa
    60301 aggtttcgga tgagcgggta aaatcatctg agtttatcca attgacgttt acgttggctt
    60361 ggactaatcc agttgattga atttaagatt gtttcgttaa tacattcagt ttgttgttga
    60421 atggcggcta cagcagcttg tgtcagcaaa cgagaaagct ctccaatata tccttcactc
    60481 atgccatata actttgtagc caacgaaatt tcatgtaaat ccgacggctg actaagcggc
    60541 agcatccgtt caaagctcat caataaacgc ataaaatcct tatttaattc ccaacgagga
    60601 agcaccgcag gttcaaaacg gttggccaac tgtgcatcag tttgaatggc tcgatatgcg
    60661 tctttaatac ctacacctac aatatgcttc gctgtattcg ccagataagc cattttgttg
    60721 atatggcaat aaaaagcgta cattgcataa ataatcagta tgaatctcta actctgtaat
    60781 cagtttaaaa tgccaaccgt gtaatttgca aaaatgtaag gcagcctgaa atttaggttt
    60841 gagttcacac cagtttttac gtaaatcgct acgatatttt acttcgtaaa taattacatc
    60901 attttgataa tgaaccaaaa catcaggggt atagatatgc ttctttcctg ctgggtcaaa
    60961 ccactcaatc gtgataggct gtacttcgaa atgctgcaca ttagaatcaa attccagcag
    61021 agttaaaaaa tcacgctcca aagatgactc ataagctgca atgcctatag ctttgttata
    61081 tggagcgatg cctgtaatat ttcgatagtt tttaggaatt ttacggattg gcatagtttt
    61141 atatgtgtag tagaaaaagg attaaaagga agacaatctt aatgagttaa tgccctttat
    61201 caataaccca aactcaatta gctttcttat aatttagtct caaataacaa tattattttc
    61261 aaatcttaat gcaaaatttt tgctctcagt cgcaaataac gttattagaa gtataagatt
    61321 ttgcatattc tagtcgcaaa caacgttgga attcacagcg aaagccacgg cggttgcggg
    61381 catcattaaa cccggaacac cgcgccaaag tgaagaacga ttccctgcgc ctgccgcgcc
    61441 gtttgatgta aacaacatca atttgaattg tccatgccgt ctgaaagccc ttcagacggc
    61501 atgatttatt cccaaaatcc gataatctca aaagaatctg tcagttaatg agaacgattc
    61561 attataaatt ttgataaaaa accgtgctct atttgttttt tgctatggtt aataagtgta
    61621 aatgttcttt attgatgaga tggattctta gttttagcag caaaaaaaaa cgataagaaa
    61681 cagagcattg atttggaagg catcggggaa tacgccctat ttcttttgat aattgtttgt
    61741 attttaaata gaaaatttgt catgccgaca gcttttgcat acaatgcacg catcccgaat
    61801 agcgacacag atgctgttca gacataccga aaaccaagtt acggaaagag agatattatg
    61861 caaggcaatc aagctgttgt tgattatatg aacgaattgc tgtccggaga gctggcggca
    61921 cgcgaccaat acttcatcca ctcccgcctc tactccgaat ggggctacac caaacttttt
    61981 gaacgtctca atcacgagat ggaagaagaa accacacacg ccgaagactt catccgacgc
    62041 atcctgatgc tgggcggtac gccgaaaatg gcgcgcgccg agctgaatat cggcacggac
    62101 gtggtttcct gcctcaaagc cgacctgcaa accgaatacg aagtgcgcga cgctttgaaa
    62161 aaaggcatca aactgtgcga agaggctcaa gactatgtta cgcgcgacct gatggttgcc
    62221 caactgaaag ataccgaaga agaccacgcc cactggctgg aacagcagct tcgcctgatc
    62281 gagttaattg gcgaaggcaa ctactaccaa agccaactgt aatccgtatt cggaataaaa
    62341 ggagtccgta tgaaaggcga ccgtctggtt atccgcgagc taaacaaaaa cttaggcttg
    62401 ctgctggtaa ccatcaacca atatttcctt cacgcccgta ttttgaaaaa ctggggcttt
    62461 gaagaactgg gcgaacattt cttcaaacag tccatcgttg aaatgaaagc tgccgacgat
    62521 ttgatcgagc gcattctgtt cctcgaaggt ctgccgaacc tgcaagaact gggcaagctt
    62581 ctgattggcg agtccaccga agaaatcatt gcctgcgatt tgaccaaaga acaggaaaaa
    62641 cacgaagcat tgattgcggc aatttccgtg gcagaagcgc aacaggatta tgtcagccgt
    62701 gatttgttgg aaaaacaaaa agacaccaac gaagaacaca tcgactggct cgaaacccag
    62761 caggaactaa tcggcaaaat tggtttgccg aactacctgc aaacagcggc gcaagaggac
    62821 taaaacacaa accactgcca aatacagcag tcccctgcat catcagacgg catatccgta
    62881 taaacgggta tgccgttttt ctttgccggt ttgcgaataa aatctaaatt gatttgaatc
    62941 aatactcgat aacggttttt gtttgataat accgatatga aatctagatt gcttgtttgc
    63001 tgtttcccaa tctgtcttga ttttacctct ttcctcttga tgtgtgtgtg tttgggtgtg
    63061 gctgccgcca cccctttttt ttggctttta tgtgaagtaa aatccgtaac agcaaatgct
    63121 taactttaac attcatccgg aagcaaatga tgggcaacaa attgactctg cctgccgaac
    63181 tgcctgacga acaggatttg cgggcggtat tggcatacaa tatgcggctt ttccgcgtga
    63241 acaagggttg gtcgcaagaa gaattggcgc ggcaatgcgg tttggacagg acttatgtgt
    63301 cggcagtcga gcgcaaacgc tggaacatcg ccctgtcgaa tatcgaaaaa atggcggcgg
    63361 ctttgggcgt ggcggcgtat cagttgctgc tgccgccgca ggaacggttg aacctgatga
    63421 ccaattccgc cgatacccga caaatgccgt ctgaaagagg tatttgaccg ccgaaccatc
    63481 cacctccctt ttcccgccaa tcgaaagaat aaaaatgcct gcaaacctgt cttcagcact
    63541 ggaaaccttc aaacggcagc gcgatgccgc cgaagcgcat tatttgaaag ccaaccgcgt
    63601 gtcggtattt ttcagggaat atacggcggc agtcgaaacc ttactggcgg cattgtgggt
    63661 agaacatttt caaaacagcg cgttatgcct gatggcggtg ggcggcttcg ggcgcggcga
    63721 actgtatccc tgttcggatg tggatttggc ggttgtctcc cctgccccgc tttcagacgg
    63781 cattcaggaa cagattgccc ggtttgttca aaccctatgg gactgcaaac tgatgccgtc
    63841 tgtaaaaagc ggcggcattg gcgaactgtg cgaaagcgtg cgcgacgata ttacggggga
    63901 cacggcgttt ttagaggccc gttttttgtt cggcaaccgc caaacggcgg acgaactggc
    63961 gaaaaaaatg aacgcgcagc gcaatgtgcc ggcgtttgtc gaggcaaaac tggtggagat
    64021 ggaacaccgc cacgccaaat cgcaaggctc aggggcggta ttggaaccaa acatcaaaag
    64081 ctgtccgggc ggtctgcgcg acatccacac cctgctttgg atagcgaaag cacaaggctt
    64141 ggcgaccaac ctgcccgacc tgctcaaaca gcggattttg acgcgtgccg aagccggtat
    64201 gctttcgcac ggctaccgcc gcctcgccca catccgcatc catctgcatt taaacgccaa
    64261 tcgcgccgaa gaccgcctgc tgttcgattt gcagccgcaa gtcgccgaaa gtatgggtta
    64321 tcaaggcttg aacctccgcc gccaaagcga agaactgatg cgcgtgtttt accgcgcgat
    64381 taaaaccgtc aaacaactgg gcggcatcct cacgcctatg ctgcgaagcc gcgtttcctc
    64441 cgcgccgatg cacgttaccc tgcggattga cgacgactac atccaagtca acaaccaaat
    64501 cgccgcgcgg cacaccgata tttttttgag gcagcccgaa cacattttca aaatcgtcga
    64561 aattatgcag cagcgcaacg acattaccgc gcttgaaccg caaaccctgc gcgcctggtg
    64621 gggcgcgacg cgcaaaatca accgcagctt ctaccaaaat cccgaaaacc gccgccgctt
    64681 cgccggtttt ttccgcaacg gcaacgggct gacccagacc ctgcgctttc tcaacctcta
    64741 cggcgtgttg ggccgctacc tgcccgcgtg ggaaaaaatc atcggcctgc tccaacacga
    64801 cctgttccac atctatcccg tggacgacca catcctcacc gtcgtccgca acgtccgccg
    64861 ccttgccttg gatatgcaca gccacgagct gccctacgcc tctgcactga tgcagtcctt
    64921 tgaaaaacaa gacatcctct accttgccgc ctttttccat gacatcgcca aaggacgcgg
    64981 cggcgaccat gccatacaag gcatcgcaga cgcgcgccaa tttgccgctg accacttcct
    65041 gaccggagaa gaaagcgacc tgctcgcttg gctggtcgaa aaccacctgc tgatgtctgc
    65101 cgtcgcccaa aaagaagaca tccaagaccc cgacgtactc gatgctttct gcaaacgcgt
    65161 ccaaacccac gaacgcctca gcgcgctcta ccttctgacc atttccgaca tacgcggtac
    65221 caatcccaag ctgtggaacg catggcgcgc cagcctgctg gaaagcctct tccatgccgc
    65281 cggacgctac cttacaggca acggcggcaa cccgcacacc ctcttcggcc gccgccggca
    65341 ggaagccgcc gacttactca cccgcgccgc cgtccccgaa aaacagcaga aaaaactatg
    65401 gaacgcgctc ggttccgcct acttcgcccg ccaccagtcc cgcgaaatcc tgtggcacgc
    65461 cgccaacctc gtacacgatt ttgaaacccc catcgtccgc agccgcatcc tgccccaaag
    65521 cgacagcttc caagtgatgg tctttatgcc caacggcccg cgcctgttcg cccgcctctg
    65581 ccgcatcttc agccgccacg gcttcgacat tctcgccgcc cgcgccttca tcaccgaaca
    65641 cgactacatc ctcgacacct tcatcgtgca aatcccctcg cagcacgctc ccgaagacta
    65701 ccccgacatt caaagcgcgc tcgaagccga actcaacagc tttatccacg gacacaccgt
    65761 tgccgaaacc tccagttaca gccgccgcat cagccgccgc agccgctata tgccgatagc
    65821 gccaagcatc accatcaccc ccgaagaaga ctatccagac tggtactccg tcgaaatcac
    65881 tgccgtcaac cgccccttcc tgctcgccga tatggcggaa gtctttttcg cccacaacgt
    65941 cagcctgcgc tacgccaaaa tctccacgct ggacgaacgc gccgaagaca gttttaccgt
    66001 ttttagccta gacctgaaaa acccaaaaat ccagtcctca ttgaagcagg ctttgctgga
    66061 acagttgaca taagcaccct gccggttttc agacggtatc ccgccgccgc gccgtatgcc
    66121 gtctgaaagc gggggatatt gcaaacaatt tattaacata atgatttata tgaatattat
    66181 tatggactca acaagaaaat ttgggatttt atgggaataa aactctgaat gtaatggttt
    66241 tatttatgga aaaattcaga taataatagg agagaatata tatcctaaga tatgcccata
    66301 tggatatttt acgctaaata cagtttttaa tagtttgaaa tcatcctttg aagagaagta
    66361 ttatgcaggt ggaaacaatg gtttagattt tggagagcaa ctatttgata tagataaata
    66421 taattctttg gaattatgca atatattttc tattgatact acatatatga gtggaggggg
    66481 taattgtgaa atagattgtt tagtattaga aatgggatat tctggtgagg aagagagact
    66541 tttttatagt tttgacaatg ggaaaaactt taaagaaatc aggtataaga aaggtacagt
    66601 tgaatctgtt atttttcaat tgaatctata atatatgggg ctgtaactag attagcagat
    66661 atgttaccct cgaaatatca agataatata ctgaaaatta aaggaaaaaa taaataaaga
    66721 actactccgt ttttttgtgc tggaagttac cgcccgatat agggcttcaa taactgagcc
    66781 acttaccaaa aattagaaaa aagcttttcc tgaaattgaa agaacgggag caacagtggt
    66841 cggcaaagga aaaccgggct attcaaaggg gaccaaaatt aaacctgata aactgtcaag
    66901 ccgccggtaa cgtgctataa tgccgtgcat cttgcacaaa ctgaaagacc gagcattatg
    66961 cgtatcgtag aaaaagccta tactttcgac gatgttttgt tggttcccgc acattcgacc
    67021 gtgctgccgc gagacgttaa acttcaaacc aagctcaccc gcgaaatcac actcaacctc
    67081 cccctgcttt ccgccgcgat ggacactgtt accgaggcgc gcctcgccat ttcgatggca
    67141 caagaaggcg gcatcggcat catccataaa aacatgccgc ccgaaatgca ggcgcgcgcc
    67201 gtttccaaag tgaaacgcca cgaaagcggc gtggtcaaag accccgtaac cgttgcaccg
    67261 acaacgctca tccgcgaagt cttggaaatg cgtgcgcagc gcaaacgcaa aatgtccggc
    67321 ctgcccgtcg ttgaaaacgg caaagtcgtc ggtatcgtaa ccaaccgcga cctgcgtttt
    67381 gaaaaccgcg tcgatttgcc cgtttccgcc attatgaccc cgcgcgaacg tctggttacc
    67441 gtccccgaag gcacaagcat agacgaagcg cgcgaactga tgcacacgca caaagtcgag
    67501 cgcgttttgg ttctgaacga aaaagacgaa ctcaaaggtc tgattaccgt caaagatatt
    67561 ttaaaaacca ccgagtttcc caatgccaac aaagactccg aaggccgtct gcgcgtcggt
    67621 gcggcagtcg gcaccggcgg cgacaccgaa gagcgcgtca aagccttggt tgaggccggc
    67681 gtggacgtga ttgtcgtcga taccgcccac gggcacagcc aaggcgtgat cgaccgcgtg
    67741 cgttgggtca aaaaaaccta tccgcacatc caagtcatcg gcggcaacat cgccactgcc
    67801 aaagccgctt tggatttggt cgctgccggc gcggatgccg tcaaagtcgg tatcggtccg
    67861 ggatcgattt gcaccacccg tatcgtggca ggcgtgggcg tgccgcaact gaccgccatc
    67921 cacaacgttg ccgaagccct caaaggcacg ggcgttccgc tgattgccga cggcggcatc
    67981 cgcttctccg gcgacatcgc caaagccctc gccgcaggcg cgtacagcgt catgctcggc
    68041 ggtatgtttg caggcacgga agaagcaccg ggcgaaatcg aactctacca aggccgctcg
    68101 tacaaatcct atcgcggtat gggttccttg ggcgcgatga gccaaggttc tgccgaccgc
    68161 tacttccaag acaaaaccga cagcaccgac aaatacgtcc ccgaaggcat cgaaggccgc
    68221 gttccttaca aaggtccgat tgtgaacatc atccaccaac tgaccggcgg actgcgctcc
    68281 agcatggggt atttgggttg cgccaatatt gctgaaatgc acgaaaaagc agaatttgtg
    68341 gaaatcactt ccgcaggtat gagcgaatcg cacgttcacg acgttcaaat taccaaagaa
    68401 gcaccgaact accatcgctg atttgaacag ccttttcaag gaaaaatgcc gtctgaaacc
    68461 tgattttcgg gtttcagacg gcattttttg tccgttcaag gcaagccgca cgggctgatt
    68521 gcgcggacgg gcaaagcaat tcggacggac acgccgaaag aatgaaaatg ccgtctgaac
    68581 gccgtttgcg ccccgtctgc cgaataccgc cggcctttaa aacggcggtg gaaataaacg
    68641 caatctttca atacgggcgt ttcgattggc ctgaaatgat gcgccgtccg cccacatcaa
    68701 aaaatcgggt ggaggcttcg ggaaaatatg ccccgcctcc tcaagccgcc ccgtcagcat
    68761 tatgatttgc ctttccgttt ttttacctta atcggaaccg gtttctttgc ctttttcctc
    68821 gattctgcgg cggcagacgc gccccttcct cttactttgc cgcctcgggc tgtttttttc
    68881 tcggcggcgg ttttcggctt ccctttcccc gccgtccctg ccggtttggc agacgcggat
    68941 gatttaactt tccgccccct gccgctcccc ccggcaatca ggacaaaatc gatttttccg
    69001 tcatccaaat cggcacgggc gacccggacg gcaaccctgt cccccatgtt gaaacggatg
    69061 ccgctgcgtt cgccttcgat tgccatgatt tcggggcgga agttgaaata gtcttcgccc
    69121 aaatcgctga tatgcaccaa gccgtcaatg tggatgccgt ccagtgttac aaagatacca
    69181 aaactggtca tgccggagat tttaccttcg aatacttcgc cgaccttatc gcgcatataa
    69241 taggttttca gccagttttc cacgtcgcgg ctggcgtcgt cggcacggcg ctcacagaac
    69301 gaggtatgca cgcccaaagc ctgccagctt ttttttggcg tgtaggtttg ctgattcaac
    69361 acggctttga tggcgcggtg tacggtcagg tcgggatagc ggcggatggg cgaggtgaag
    69421 tgggcgtatg cttcgtaggc aagaccaaag tgtccgtcgc aatgcggttc gtaaaccgcc
    69481 tgctgcatgg agcgcaacat catgacttgc agcaattcgg catcaggtct gcctttgaat
    69541 tgttcgacaa gcgcggcata gtctttcggc gacgggttgt cgccgccgcc aagttgaagc
    69601 cccaacagac cgagctgctc gcgcagggtg gcgagttttt cgggcgtggg gcccaaatgg
    69661 ttgcggaaca aagccgtatg cttgtttttc aacaggaaat ccgctgcgca aacattcgcc
    69721 gccagcatac attcttcaat cagcttgtgg gcatcgttgc ggacaacggg gacgattttt
    69781 tcgattttgc cgttgtcatc gaaaatcatc tgggtttcga cgctttcaaa ctccaccgcg
    69841 ccgcgttcga aacgcttttt ctgaaggatt ttgaagagtt tgtaaagggt gtcgatttgg
    69901 gctttgtacg gatggtcgat gccgtctgaa atccatttcc aaacttggtt gtaggtcagg
    69961 cgggcatgag agcgcattac ggcggggtag aagcggtatt ctttgatatt gcccgcatag
    70021 gtaacgacca tatcgcacac catacacaaa cgctcgacat cgggattgag cgagcaaatg
    70081 ccgttagaca ggttttccgg cagcatcgga atcacacggc gcgggaaata tacgctggta
    70141 ctgcgttctt gagcatctgc atcaatcaca tcgtcagggc ggacataatg gctgacatcc
    70201 gcaatcgcca cgaccagacg gtaattgcgt ccgacttttt cggcaaacac cgcgtcgtcg
    70261 aaatcgcgcg ccgtttcgcc gtctatcgtt accaaaggca ggtcgcgcaa atcgacgcgg
    70321 cctttcaaat cgcttttgcg tacatggacg ggaatttttt tcgcagcttt ggcacacgct
    70381 tcactgaatt ggtgcggcaa atgatgcttg cgcacggcaa tttcaatctc catgccgctg
    70441 tcggcataat cgcccaaaac ttcgatgatt tttgccactg ccggccggtt ttgctcagga
    70501 taaacctcaa tttcgccgac gatgacctga ccggattcag gtttgaaacg cgccacgccg
    70561 tccggttcca atacgatgct ttggttcaga cgcttgtctt ccggctccaa aatcgccacg
    70621 cccctatcca tatagaaacg gccgaccact ttgctttgcg cgcgttcgac aatatccaga
    70681 actgtccctt cgcggcggcc cctacggtcc atgccggcag gacgaacagt gacaatatcg
    70741 ccgtgcataa tgccgcgcat ctggcgttca tacaaaacaa aatcaccgtc tttggcgggc
    70801 gtgagcggca cggcaaaacc gaaaccgtcc ttatgcgcct cgacgcggca tttgaccaaa
    70861 tccaatttgt ccgccgcgca aaccgcgccc cgacggttga ttaaaacctg accgtcccgc
    70921 gccatcgcct tcagacggcg ttcaaaaaag acatactcgt cttccgtaat cgacagctcg
    70981 cgcgcaagcg attcgatttt tgaaggcaca cctttgcgct ccaacaattc gattatccat
    71041 tcccgactgg gcaaaggatg ttcataacgc tgtttttcac gacttaaaaa cgggtctttt
    71101 tcccgtaaat ttaaagattt aatattttta ttcatttgga ctgttgacat tctctttatg
    71161 aactatataa tagcgacttc tttgcggcag gacactgtac cgcaaaacag caaataaagc
    71221 aaagcccggg tggcggaatt ggtagacgcg ctagcttcag gtgctagtat cctcacgggt
    71281 gtggaagttc gagtcttctc ccgggcacca aatcaaatgc tttgctttat ttcaatattt
    71341 agattgcccg ggtggcggaa ttggtagacg cgccagcttc aggtgctggt atcctcatgg
    71401 gtgtggaagt tcgagtcttc tcccgggcac caaataataa attatcccga ttgggataat
    71461 ttccgccatt agagaggtgg atgagtggtt taagtcgcac gcctggaaag cgtgtatacg
    71521 tgaatagcgt atcgagggtt cgaatccctt cctctctgcc aaatacaaat cccaaacttt
    71581 tacggtttgg gattttttat tattcaaaca ataatttcat tgggaaaacc cgttattgtc
    71641 tataatcgtc atcaaccgct tacaactatc cgttaccgtt ttttgtggta tgttttgcgg
    71701 tatgttttac cctcttcctc agcctgtaag gactcaaaag gcgtattcct tcaaacggca
    71761 ttgttcgaca tgctgccggg aagccgaacc cgataccgaa acgcgacggg gcgatatacc
    71821 gctggaaaaa tggcggcagt ttcttgattg ggtaggaaaa acgccgtctg aaacagcttc
    71881 aagaagccgc aaagcccgtc cgaataaaaa atgccgtctg aaatttttca gacggcatcg
    71941 cgtatcattt aaacattaat ccagtaaaca tcaatccagc tttttaaagt ggcggcggcg
    72001 ttccaattcg ctcaagtaac gcttgcgcag acggatggat tgcggcgtga tttcaacgag
    72061 ttcgtcatcg tcgataaact caaccgcacc ttccagcgtc agcttgattg gcgtggtcag
    72121 gcgaacggct tcgtcggtac cgctggcacg gatgttggta agttttttgc ctttgagcgg
    72181 gttgaccacc aaatcgttgt cgcgactgtg gatgccgata atcatgcctt cgtagatttt
    72241 gtcgttgggc gatacgaaca tacggccgcg gtcttccaga ttccacaagg cgtaagcgac
    72301 tgcctcgccc tgctcttggg acaccagcac gccgttgtgg cggccgggca tatcgggttt
    72361 gacgggcgcg taatcgtcga acacgtggct catcagcccg acaccgcgcg tcagggtcat
    72421 gaattcgcct tggaaaccga tcaagccgcg cgctggaata tggtattcga ggcgggtgcg
    72481 tccgttgcca tcgctttcca tattggtcag ttcgccacgg cggcggccga gttcttccat
    72541 taccgcgcct tggttgtcgt cgggtacgtc tacggtcagg ttttcataag gttcgcattt
    72601 ttgaccgtcg atgtcgcggt acacgacgcg cggtttgccg acggcgagtt cgtagccttc
    72661 gcggcgcatg ttttccagca aaatggtcag gtgcagctcg ccgcgcccgg atacgcggaa
    72721 cacgtcggca tcggcggtat cttccacgcg cagggcgacg ttggtcagca attctttttg
    72781 caggcggtcg cggatttggc ggctggttac gaatttgcct tccgtacccg ccagcgggct
    72841 ggtgttgacc ataaagtcca tcgtcagcgt cggttcgtcc acgctcaaca tcggtaggcc
    72901 tttgggattg tctttgtcgg tgatggttac gccgataccg atgtcttcga taccggaaat
    72961 aatcacgatg tcgccggctt cggcttcttc aagcggcacg cgttccaaac ctttgaaacc
    73021 caaaagctgg ttgatgcggc cttgggcgat ttgctgatcg tggttcatga cggcaacggt
    73081 ttggccgggt ttgattcgtc cgttcaagat acgaccgata ccgaggcggc cggtgtagtt
    73141 gtcgtagtcg agttgggaaa tttgcagttg cagcgtttcg tccgcgctgc cgctcggtgc
    73201 aggcgtatat tttaagatag tatcgaacag cggacgcatg tcgttgctct cgtcggtttc
    73261 ttccaatttg gcgaaaccgg acagaccgga agcataaaca atcgggaaat ccaactgctc
    73321 gtcggtcgcg cccaagttgt cgaacagctc gaaagtttgg tcgatgaccc agctcggacg
    73381 agcggacggc ttgtcgattt tgttgatgac gacaatcggt ttcagcccca aagccaaggc
    73441 ttttttggtc acgaaacggg tttgcggcat cgggccttcc tgcgcgtcca ccaacaagac
    73501 gacgcagtcc accatcccca aaacgcgctc tacttcgccg ccgaagtcgg cgtgtcccgg
    73561 cgtgtcgacg atattgatgt ggtagccttc gtaatcgatg gcggtgtttt tggcgaggat
    73621 ggtgatgccg cgttcttttt cgaggtcgtt gctgtccatc acgcgctcgt caacctgctg
    73681 gttggcgcgg aatgtgccgg attggcgcag cagttggtcg accaatgtgg ttttgccgtg
    73741 gtcgacgtgg gcgatgatgg cgatgttgcg gatttgtttc ataattaatg tttttcaaaa
    73801 actgtaagag ataactgcgc attataccac gctttaccgg acagtatttg ggaaatgaag
    73861 atgccgtctg aagcccttgc ctttc
//
LOCUS       DAWFIE010000002        72458 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWFIE010000002 DAWFIE010000000
VERSION     DAWFIE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 72458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.002"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(67..3166)
                     /locus_tag="WGQ71_000081"
                     /pseudo
     CDS             complement(67..3166)
                     /locus_tag="WGQ71_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(3203..3306)
                     /locus_tag="WGQ71_000082"
                     /pseudo
     CDS             complement(3203..3306)
                     /locus_tag="WGQ71_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3351..4568)
                     /locus_tag="WGQ71_000083"
     CDS             complement(3351..4568)
                     /locus_tag="WGQ71_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0706169.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGIADGYKGRLKDVVWKTLGEVFD
                     LKNGYTPSKSNKEYWENGSIPWFRMEDIRENGRILDNSLKHISKSAVKGGKLFPAKSI
                     MMSTTATIGEHALIKVNYISNQQLTNFTIKDEFKDALDINFAFYYFFIIAEQSKKLIN
                     TSSLPIISMKELKKLKIPIPPLPEQEKIAAILDKFDTLTHSISEGLPHEIALRRKQYE
                     YYREQLLAFPKAT"
     gene            complement(4734..5744)
                     /locus_tag="WGQ71_000084"
     CDS             complement(4734..5744)
                     /locus_tag="WGQ71_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ0706170.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTVAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(5741..7285)
                     /locus_tag="WGQ71_000085"
     CDS             complement(5741..7285)
                     /locus_tag="WGQ71_000085"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ0706171.1"
                     /translation="MMTEIQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     SAEIEA"
     gene            complement(7358..8362)
                     /gene="rfaD"
                     /locus_tag="WGQ71_000086"
     CDS             complement(7358..8362)
                     /gene="rfaD"
                     /locus_tag="WGQ71_000086"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ0706172.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMDNNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
                     "
     gene            complement(8403..9521)
                     /locus_tag="WGQ71_000087"
     CDS             complement(8403..9521)
                     /locus_tag="WGQ71_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0706173.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPLIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     GEKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFNQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(9523..10758)
                     /gene="dcm"
                     /locus_tag="WGQ71_000088"
     CDS             complement(9523..10758)
                     /gene="dcm"
                     /locus_tag="WGQ71_000088"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0706174.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDASKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(10795..11766)
                     /gene="hldA"
                     /locus_tag="WGQ71_000089"
     CDS             complement(10795..11766)
                     /gene="hldA"
                     /locus_tag="WGQ71_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ0706175.1"
                     /translation="MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGDDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKQKYLEVLSEYDAIIFSDYGK
                     GGLSHISDMIDCAKHEGKIVLIDPKGDDYEKYAGATLITPNRAELKEVVGSWKNEGEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELTKALSGQSTM"
     gene            complement(11822..12562)
                     /gene="pyrF"
                     /locus_tag="WGQ71_000090"
     CDS             complement(11822..12562)
                     /gene="pyrF"
                     /locus_tag="WGQ71_000090"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ0706176.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRSLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNIAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAANPLAVLREVNRVANLEAN"
     gene            complement(13088..13735)
                     /gene="adk"
                     /locus_tag="WGQ71_000091"
     CDS             complement(13088..13735)
                     /gene="adk"
                     /locus_tag="WGQ71_000091"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ0706177.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            13950..14789
                     /gene="htpX"
                     /locus_tag="WGQ71_000092"
     CDS             13950..14789
                     /gene="htpX"
                     /locus_tag="WGQ71_000092"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ0706178.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(15090..15650)
                     /locus_tag="WGQ71_000093"
     CDS             complement(15090..15650)
                     /locus_tag="WGQ71_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706179.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(15764..16156)
                     /locus_tag="WGQ71_000094"
     CDS             complement(15764..16156)
                     /locus_tag="WGQ71_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0706180.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(16364..16756)
                     /locus_tag="WGQ71_000095"
     CDS             complement(16364..16756)
                     /locus_tag="WGQ71_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0706181.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(16873..17283)
                     /locus_tag="WGQ71_000096"
     CDS             complement(16873..17283)
                     /locus_tag="WGQ71_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0706182.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(17297..17734)
                     /locus_tag="WGQ71_000097"
     CDS             complement(17297..17734)
                     /locus_tag="WGQ71_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0706183.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(18145..19443)
                     /locus_tag="WGQ71_000098"
     CDS             complement(18145..19443)
                     /locus_tag="WGQ71_000098"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ0706184.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMEGWKESTFGIKNYEE
                     LPDNAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(19547..20698)
                     /locus_tag="WGQ71_000099"
     CDS             complement(19547..20698)
                     /locus_tag="WGQ71_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0706185.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            21033..22412
                     /gene="norM"
                     /locus_tag="WGQ71_000100"
     CDS             21033..22412
                     /gene="norM"
                     /locus_tag="WGQ71_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ0706186.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            22561..23997
                     /locus_tag="WGQ71_000101"
     CDS             22561..23997
                     /locus_tag="WGQ71_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0706187.1"
                     /translation="MFIKSICLSNFKGFIGDNHQIKFKIPDGATAGSGLNIFIGENNS
                     GKSSIFEAINFLRNGIKEDEAYRIKSKLADDTQPNDACVELAFCGNIQNAITYFVQRN
                     KQAAFNNAINNEQNLKAKRNTVSCKKLDLWDNESQDYSNPSGIDAPFKALFETNFIWA
                     DTNPNDEAAFGSTTLCGLLLKEIAQAHINSAEYQTFRDSFNQVFNSPASNLRQQIATI
                     EQKVQRIFTEQFGQADIHFRFDELKIDSFFKNASIFIDDGVDIPMSEKGNGMQRSVAL
                     ALLQVYAEELAHDEEQGQTKPFYLFIDEPELCLHPKGQTKLLEALLEISRTKQVFLTT
                     HSPYFLVTPHLSNVGLFIFRKDGISNIVEDASLEPMFPWSPTWGEINFKAYKLPTVEL
                     HNELYGKLQHDCGQFREKDFEQWLQSRNIQFTKQWIRENNGSAQAPYDVTLQTFIRNH
                     IHHPENRRNALYTENELRQSIEEMIRLL"
     gene            complement(24263..24637)
                     /locus_tag="WGQ71_000102"
     CDS             complement(24263..24637)
                     /locus_tag="WGQ71_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0706188.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKSTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYFAERQNHINGIENFWNQAKRHLRKFNGIPKAHF
                     ELYLKGYERRFNNSEIKVLVPF"
     gene            24712..25752
                     /gene="murB"
                     /locus_tag="WGQ71_000103"
     CDS             24712..25752
                     /gene="murB"
                     /locus_tag="WGQ71_000103"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ0706189.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADELCDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYAGLVVHMENKGIREIARSDGIVRIEAQAGEIWHDFVLHT
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVIGAEKAAALLEQHPDMPHYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHGRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            25764..26414
                     /locus_tag="WGQ71_000104"
     CDS             25764..26414
                     /locus_tag="WGQ71_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ0706190.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRAEYDRKIGNGNL"
     gene            26469..27245
                     /locus_tag="WGQ71_000105"
     CDS             26469..27245
                     /locus_tag="WGQ71_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0706191.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVSAVKRSLTSDDI
                     NLPIHLDTIDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARTCN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGVPNIDILRLGG
                     LYCAERHPVGRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            27296..27862
                     /locus_tag="WGQ71_000106"
     CDS             27296..27862
                     /locus_tag="WGQ71_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ0706192.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLAAGNGFADTEITFRNSAIQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGRFSREPESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            27882..28772
                     /locus_tag="WGQ71_000107"
     CDS             27882..28772
                     /locus_tag="WGQ71_000107"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ0706193.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            28797..29555
                     /locus_tag="WGQ71_000108"
     CDS             28797..29555
                     /locus_tag="WGQ71_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0706194.1"
                     /translation="MNPKNPPDHTEDAVRLSKRMAQLGLCSRREADGYIGQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NRWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(29610..30275)
                     /locus_tag="WGQ71_000109"
     CDS             complement(29610..30275)
                     /locus_tag="WGQ71_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0706195.1"
                     /translation="MTVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(30476..31372)
                     /locus_tag="WGQ71_000110"
     CDS             complement(30476..31372)
                     /locus_tag="WGQ71_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ0706196.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQYSEALDFAQLHRLTAEMVALLDSRAGKISVSFPFFRKKTAPVSGIQSLLD
                     YDVCLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            31441..32598
                     /locus_tag="WGQ71_000111"
     CDS             31441..32598
                     /locus_tag="WGQ71_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ0706197.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGATGFAFSSGMAGIDAVWRTFLQQGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKATGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMEAHCKNALEIARRLEDHPAIEKVFH
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIRVGLLRFSVGIEDVDDIWNDISAALDTTV"
     gene            complement(32662..33663)
                     /gene="hemB"
                     /locus_tag="WGQ71_000112"
     CDS             complement(32662..33663)
                     /gene="hemB"
                     /locus_tag="WGQ71_000112"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ0706198.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPAAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGDKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(33846..34130)
                     /gene="yhbY"
                     /locus_tag="WGQ71_000113"
     CDS             complement(33846..34130)
                     /gene="yhbY"
                     /locus_tag="WGQ71_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ0706199.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            34240..34860
                     /locus_tag="WGQ71_000114"
     CDS             34240..34860
                     /locus_tag="WGQ71_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ0706200.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            34924..36891
                     /gene="ftsH"
                     /locus_tag="WGQ71_000115"
     CDS             34924..36891
                     /gene="ftsH"
                     /locus_tag="WGQ71_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ0706201.1"
                     /translation="MGNTFKSILAWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASSESEQRSENKA"
     gene            37112..37543
                     /locus_tag="WGQ71_000116"
     CDS             37112..37543
                     /locus_tag="WGQ71_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ0706202.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            37536..37814
                     /locus_tag="WGQ71_000117"
     CDS             37536..37814
                     /locus_tag="WGQ71_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ0706203.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            37867..38445
                     /gene="pth"
                     /locus_tag="WGQ71_000118"
     CDS             37867..38445
                     /gene="pth"
                     /locus_tag="WGQ71_000118"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ0706204.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAIAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            38506..38712
                     /locus_tag="WGQ71_000119"
     CDS             38506..38712
                     /locus_tag="WGQ71_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ0706205.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            38709..39071
                     /locus_tag="WGQ71_000120"
     CDS             38709..39071
                     /locus_tag="WGQ71_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ0706206.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            39302..40717
                     /locus_tag="WGQ71_000121"
     CDS             39302..40717
                     /locus_tag="WGQ71_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HEZ0706207.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMIPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVALLRLSRGSMKAAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKERKTYVFVLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            40836..41345
                     /locus_tag="WGQ71_000122"
     CDS             40836..41345
                     /locus_tag="WGQ71_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0706208.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            41508..42890
                     /locus_tag="WGQ71_000123"
     CDS             41508..42890
                     /locus_tag="WGQ71_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ0706209.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIGVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLSIIEKDGFAAAGKQGSVTEKDISGEYHNHI
                     VGHVKLKRPMNIAIDAGNGVGGAFAGKLYRALRCGVTELFCDVDGNFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSSMKKTGALVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIDLPEGSNGHKVIEELAAN
                     AKFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(42910..43665)
                     /locus_tag="WGQ71_000124"
     CDS             complement(42910..43665)
                     /locus_tag="WGQ71_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0706210.1"
                     /translation="MIKIRNIHKTFGKNTILRGINLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVNLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLDTMKELAQEGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPQDLFNHPKHERTRRFLSQIQSTKI"
     gene            complement(43675..44391)
                     /locus_tag="WGQ71_000125"
     CDS             complement(43675..44391)
                     /locus_tag="WGQ71_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0706211.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(44381..45211)
                     /locus_tag="WGQ71_000126"
     CDS             complement(44381..45211)
                     /locus_tag="WGQ71_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0706212.1"
                     /translation="MMLKKFVLGGIAALVLAACGGEGGSASSSAPAQSAGASGDSLIE
                     RINNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMM
                     AGLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKT
                     AQSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAG
                     VKIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ
                     "
     gene            45496..46404
                     /locus_tag="WGQ71_000127"
     CDS             45496..46404
                     /locus_tag="WGQ71_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ0706213.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            46484..50098
                     /gene="recB"
                     /locus_tag="WGQ71_000128"
     CDS             46484..50098
                     /gene="recB"
                     /locus_tag="WGQ71_000128"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ0706214.1"
                     /translation="MSAPIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAKLGDDTLSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLIPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGYPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLADNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADTPLAYLIEGSPQ
                     DSRETVRQTYAQEKDGIAMLKRNWRRLADNAPAGTDFVFTEDAPPPAAYRGNADQTAE
                     FAANSIPERGFRFVRHTSFTTLSRHTQTPDGSEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHNFPKGTQAGLCLHEILENFKFGQAAAGQETFITDKLKKYGFEEIWLPAVAEM
                     AEACQKTPLTGAYGLSDILSECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDADTLEQIK
                     "
     gene            50137..50496
                     /locus_tag="WGQ71_000129"
     CDS             50137..50496
                     /locus_tag="WGQ71_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ0706215.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            50621..51100
                     /locus_tag="WGQ71_000130"
     CDS             50621..51100
                     /locus_tag="WGQ71_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ0706216.1"
                     /translation="MKHILPLIAASALCISTASAHPAGEPSTPNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIIFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(51171..52550)
                     /gene="radA"
                     /locus_tag="WGQ71_000131"
     CDS             complement(51171..52550)
                     /gene="radA"
                     /locus_tag="WGQ71_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ0706217.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAALEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            52907..54031
                     /locus_tag="WGQ71_000132"
     CDS             52907..54031
                     /locus_tag="WGQ71_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0706218.1"
                     /translation="MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGYETVWAND
                     FSHWACESFRKNIGDVIVEGDIEQIDPNNPTIPDCDIILGGFPCQDFSMIWKQPGLEG
                     ERGNLYKSFLRFVNAKKPKVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYN
                     FAEFGVPQFRERVLIVGVRLDTGFDFHHPEPTHGENTDYGLLPYVTAGQAISDIPENA
                     NNHEQLKISEQTKKRLNLIPEGGNYSDIPRDHPLYVKSMLSLVYRRMHRDKPSTTIIA
                     AGGGGTWGYHFPEPRAFTNRERARLQSFPDNFEFMGSTTEVRRQIGNAVPPQGVVELA
                     KAILPVFSDGYHKVNLHEKLEAEKNVFFQDRLNKIRGGKR"
     gene            54028..55068
                     /locus_tag="WGQ71_000133"
     CDS             54028..55068
                     /locus_tag="WGQ71_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoFVII family restriction endonuclease"
                     /protein_id="HEZ0706219.1"
                     /translation="MNTVFSNIANAKITDKSLNKVWMDLFMSADDMLMATGYVSNDAV
                     KELHKILEESDHKQKLSLMVGMHYLEGFSRPQYSSLCELNDFLQDEQRGMVYVSPWVK
                     FHGKMYSFQNQREIHTLIGSANLTCFWDKAERTYETMVHLDDYQIASKLREDIAQTIK
                     QLGIPINKADKPVEFKEHNVHLEQCLGVEKISKERVEKLFTQTSQYYFELPVKTEPRS
                     SLNRFFAEGRKNQRGFYLPRPWYEAELIVSTRFLTAEGYPKEKNFTVITDDGWQFECN
                     TTGQNGKNFSSKGDQTIFGKWVKGRLEATGCLKTGEPVTEETLRQYGNDHFEFRSTDN
                     PDVWLLSFKGKN"
     gene            55069..56958
                     /locus_tag="WGQ71_000134"
     CDS             55069..56958
                     /locus_tag="WGQ71_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0706220.1"
                     /translation="MLRTYLNQLTPPELADSVENTVNGFMEKLSQTEPKIAQNVLLLG
                     NVQSGKTAQVLGILSALADDGDHKVFLYLTTDSVDLQDQTVKRAKANLKNFIVLSEAD
                     DRSFMEVMKAENPILVVIKKNARVLKRWRNLFASQSSLKGYPLVIVDDEADAASLNTN
                     TDKPDKDASTINKLLNDIKNSCCQSLFIQLTATPQSLLLQNEASDWQPEFIHFFEAGE
                     KYIGGNFVFSDPPSYIVRFIDSELDDMKDESGEIAEGAKQALLSFLITCAEFALCDKA
                     NCNFALHPSYKIQDHQAFSKKIQAFLNDLVQAVNNGEDLAGSFKESYLDLQKTKPDIH
                     HFDEIYEKLTALLENKQISTLVVNSQTESDFDLERGFNIIIGGNVIGRGLTIPKLQTV
                     YYSRTAKKPNADTFWQHSRIFGYDRDKSLLRLYIPFDVYYFFVQLNQANNLIIGQAKN
                     SGGNIQVIYPKNINPTRKNVLKFDSINQIVGGVNYFPLHPNEDNLSEINKILPSILKD
                     EIQSDLYQIDIEDLFLVLDKLGRYVPDDWNKEKFIAGVEALKAQRPSFKTYVLIKTGR
                     KLSRATGTMLSEDDRKLGEKYPNDLFLTLYQVVGNKDKGWQGKDFWLPNIKLPHNGLV
                     YQSAK"
     gene            complement(57021..58085)
                     /locus_tag="WGQ71_000135"
     CDS             complement(57021..58085)
                     /locus_tag="WGQ71_000135"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ0706221.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTGLATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVSSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGNQVALQGNFDPFALFGTPESIRTEVARILVDYGQGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(58160..59377)
                     /locus_tag="WGQ71_000136"
     CDS             complement(58160..59377)
                     /locus_tag="WGQ71_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ0706222.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPEKMQRFGSARKGRKAALYLNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            complement(59374..60708)
                     /locus_tag="WGQ71_000137"
     CDS             complement(59374..60708)
                     /locus_tag="WGQ71_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0706223.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLMLDGVLKPGVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLLSPEQAYFVRENLRLRLLDARIALMQRNGEVYQGDLNNAETAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            complement(60723..61463)
                     /locus_tag="WGQ71_000138"
     CDS             complement(60723..61463)
                     /locus_tag="WGQ71_000138"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ0706224.1"
                     /translation="MLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAAGLELLSE
                     QYARADAVFWVSPTAVETAVPYLNLSDGIKMHISVGQGSRRALERCLGRTVIAPEDGN
                     DSEAVLRLTVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFRRHKPLNF
                     QNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALKREGVCSV
                     ETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(61495..61917)
                     /locus_tag="WGQ71_000139"
     CDS             complement(61495..61917)
                     /locus_tag="WGQ71_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706225.1"
                     /translation="MKKTFLSAVLLLSAATQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGWGRGRWATMKFQNGEFMYTVDAGFDSV
                     THAVGSGVLVERRGKEIARFDCTSKTAQANFDDDDFAW"
     gene            complement(62168..62488)
                     /locus_tag="WGQ71_000140"
     CDS             complement(62168..62488)
                     /locus_tag="WGQ71_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ0706226.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVRDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            complement(62511..63137)
                     /gene="upp"
                     /locus_tag="WGQ71_000141"
     CDS             complement(62511..63137)
                     /gene="upp"
                     /locus_tag="WGQ71_000141"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ0706227.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKEKGCRNIKALVLV
                     AAPEGVRAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(63243..63554)
                     /gene="grxD"
                     /locus_tag="WGQ71_000142"
     CDS             complement(63243..63554)
                     /gene="grxD"
                     /locus_tag="WGQ71_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ0706228.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(63655..63870)
                     /locus_tag="WGQ71_000143"
     CDS             complement(63655..63870)
                     /locus_tag="WGQ71_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ0706229.1"
                     /translation="MEAIHPYAYPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            complement(63896..64675)
                     /locus_tag="WGQ71_000144"
     CDS             complement(63896..64675)
                     /locus_tag="WGQ71_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0706230.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     AEVSAIAEVYEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            complement(64680..65030)
                     /locus_tag="WGQ71_000145"
     CDS             complement(64680..65030)
                     /locus_tag="WGQ71_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ0706231.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(65034..66011)
                     /locus_tag="WGQ71_000146"
     CDS             complement(65034..66011)
                     /locus_tag="WGQ71_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ0706232.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVVLIHPAESMNIQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(66045..67157)
                     /locus_tag="WGQ71_000147"
     CDS             complement(66045..67157)
                     /locus_tag="WGQ71_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0706233.1"
                     /translation="MTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGEI
                     SLQDALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            67343..68044
                     /locus_tag="WGQ71_000148"
     CDS             67343..68044
                     /locus_tag="WGQ71_000148"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ0706234.1"
                     /translation="MSLKTVTVIGAMEQEIELLREMMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPDCVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AANSAKMVAEIVKSL"
     gene            68187..69980
                     /gene="lepA"
                     /locus_tag="WGQ71_000149"
     CDS             68187..69980
                     /gene="lepA"
                     /locus_tag="WGQ71_000149"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ0706235.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            70123..71142
                     /gene="lepB"
                     /locus_tag="WGQ71_000150"
     CDS             70123..71142
                     /gene="lepB"
                     /locus_tag="WGQ71_000150"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ0706236.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            71240..71346
                     /locus_tag="WGQ71_000151"
                     /pseudo
     CDS             71240..71346
                     /locus_tag="WGQ71_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(71401..72306)
                     /locus_tag="WGQ71_000152"
     CDS             complement(71401..72306)
                     /locus_tag="WGQ71_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0706237.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGV
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEVEPVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSSH"
BASE COUNT        17699 a        19139 c        17785 g        17835 t
ORIGIN      
        1 tccactataa aaaccgcctc cgcataaaat caaaaatgcc gtctgaaagg ttcagacggc
       61 attttgtcaa atatcggtcc ctattccggc aaacttctcc acaaacgccg caatcttttg
      121 aaaaacactt tgtttcttcg tcagatattg cgggttgagc ggactcattt tcggcagggt
      181 ttcggtcagt tccgtgccgt tttcgctggc atagccgcgt ttgagcgagc cggtcaaata
      241 gcgtttggcg gcggtttcgt tcaggccttc ggcgacaatc aatcctgccg cttcgtgccg
      301 catcacctct tgcgcgtagg tgtagaaggt ttccagtatg gcgggaacgt cgggtacttt
      361 gtccaaatcc gtatcgttga tgaaatccac aatcagaccc tctttggcgc ggtggccgat
      421 gctggcgcgg atgatgcggc ggatttcttc caccagctcc gctttgcctt tgattttttt
      481 gtggtgttcg aaaaccagtt gcaggatgta atccagattg atttcctgtg atttgagcaa
      541 atccacctca aaaaccacat cgtcccaatc gatttttgac tgttctttct gttcgcctgc
      601 tttttggcgg cgcaaccaat cgcggatgtc gttgtaggcg gaacggtagt cctgcaccgc
      661 cctttcagac ggtatcggca cttgccgcat ttcctgcacg tcttcatcgc tcaagtagta
      721 tttttcttga aacgccttca tcgcatcttc gtccgccgcg tccacactct gcaactcgcg
      781 cagcgcggca aattcatcgt agttctgcaa tacgttttcc gcccgcaggt attcgccgaa
      841 gagtttggca aaatcttttt tgtctttttc cgtttcgatt tcgtcgggtt cggggaaacg
      901 ttcacgcaac tcttttgcca catccaaata accgcgccgc gcttcgccgg tctggctgtc
      961 ggtatagccg ttcatgtatt cttcgtagct tttttccagc accacgtttt tggtgtgttt
     1021 gtcgccgaac agggtaatcg catcaatggt tgcctgctcc aaatcgcgga agcagacaat
     1081 attgccgaag gttttggtgg catcgtaaat gcggttggtg cgcgaaaacg cctgcatcag
     1141 gccgtgatag cgcaggtttt tatcgacgaa cagcgtgttc agcgtcggcg cgtcaaaacc
     1201 cgtcaaaaac atgccgacca caatcagcaa atccacttcc tgatttttca cccgttttgc
     1261 caaatcgcgg tagtagtttt gaaaggcttt gctgtccgtg ccgaaattgg ttttgaaaca
     1321 ggcgttgtaa tcgttgatgg cagcctgcaa aaattctttc gcgctgccgt ccatcgcttc
     1381 cggttcaaag gtctcatcaa caatttcacc gacggcgttc tgctcttcgt tggccgcaaa
     1441 ggaaaaaatg gtggccactt tcagcgggtg caggctgcct gcctgttgtg ttttgaacgc
     1501 ttcgtaatag cacttcgccg catccacgct gctgaccgca aacatggcgt tgaagccttt
     1561 accgcccgca ttcaggcggt gcgttttctg cctgaactgg ttcaggatat attgcgtgat
     1621 ttcgcggatg cgttcggggt gcagcagggc ttggcggttt tcggcagcac tcagtttctt
     1681 ctcatcctgt tccgcttcca cggctttgaa ctgcgggcgc acatcgttgt aatccacttt
     1741 gaatttcaat actttttcat cgcggatggc atcggtaatc acataagaat gcaactcacg
     1801 cccgaacacg cccgccgtgg tttccgcgcc caaagcgttt tcgggaaaaa tcggcgtacc
     1861 ggtaaagccg aactggcaga attttttaaa tttctttttc agattttttt gcgcttcgcc
     1921 gaattgcgag cggtggcatt cgtcgaaaat aaagacaacc tgctggtgat aaaccggcag
     1981 attatcctca ctcttcatca ggttgttcag cttttggatg gtggtaacga tgattttgtt
     2041 gtcgtctttt tccaaattgc gtttcaaacc cgccgtgctt tccgaaccgt ttacgctgtc
     2101 gggcgaaaaa cgctggtatt ccttcatcgt ctggtagtcc aaatccttcc tgtccaccac
     2161 gaagaaaacc ttgtcgataa acgccgattc cgtcgccaga cgcgccgcct taaagctggt
     2221 cagcgttttg ccgctgcccg tggtatgcca aacatagccg ccgctttccg gtttgctcca
     2281 attcttcgcc tgcgccgaac tgttaatttt ccacagaatg cgttcggcgg cggcaatctg
     2341 gtacggccgc ataatcagca gcgtatcatt cgcatcgaac acgctgtaat gcagcaaaac
     2401 gctcagtaat acgctttttt tgcaggaacg tggcggtaaa gtctttcaaa tccttaatcg
     2461 gatgattgtc cgaccgcgcc caattcatcg tgaaatcgaa gctgtttttg tcgcgcttgg
     2521 tggtattggc gaaatagcgc gtgtccgtgc cgttggaaat cacgaagatt tgcaggaatt
     2581 tgaacagcga attttcgctg ttgaagctct ctttgctgta acggtgcacc tgattgaatg
     2641 cctcgcgcac cgccacaccg cgctttttca attcaatctg caccagcggc aggccgttta
     2701 ccaacacggt aacgtcatag cggtttgcat gcgtgcccgt ctgctcaaac tggttgataa
     2761 gctgcacatg gttgcgggca aggtttttct tgtccagcag ataaatgttt ttcagacggc
     2821 cgtcatcaaa agcgaaatca tagatatggt cgtcgtggat tttgcgggtt ttatcggtaa
     2881 tgttttcaga cggcctgtcc agatattccg tcaaaaaccg cgcccattcg ccgtctgaaa
     2941 acgccacatc gttcagccgc tgcaactgcg cgcgcaggtt ttccagcagc ctactttggc
     3001 tgttcaaatc cttgcggtat tcgtaaccct gattctgcaa atcggcgatt aactccgctt
     3061 ccaactggtt ttccgattgg tagctgcccg actgttcgat tttttcatat tggtcgagca
     3121 cgatgaaatt cggcgtttcg gcgatgggtt tggtttcgag gttcatatgg gtcgtccttt
     3181 gcggtgtcgg gttttatagt gaattaaatt taaaccggta ccgcgttggc tcgccttgcc
     3241 gtactatttg tactgtctgc ggctcgccgc cttgtcctga tttaaattta attcactata
     3301 aacggtttga tgttcggatt gggctgcctg aaaatgtgca ggctgctttt tcaggtagcc
     3361 ttagggaagg caagcagctg ttcgcggtaa tattcgtatt gtttgcggcg cagggcgatt
     3421 tcgtgcggca ggccttcgct gatagagtgg gtcagggtat cgaatttgtc taggatggcg
     3481 gcaatttttt cctgttcggg gagtggtggg atggggattt ttaatttctt taattctttc
     3541 atgctaataa taggtaaact tgaagtatta ataagttttt tgctctgctc cgcaataatg
     3601 aaaaaataat agaaagcgaa atttatatct aaagcatcct taaattcatc tttaattgta
     3661 aaattggtca gttgttggtt actaatataa ttaactttta ttaacgcatg ctctcctatg
     3721 gttgccgttg tcgacatcat tattgattta gcaggaaaca gcttgccgcc cttaactgcg
     3781 ctttttgata tatgcttcaa tgaattatct aaaatacggc cattttccct aatatcttcc
     3841 attctaaacc aagggattga gccattttcc caatattctt tatttgattt ggaaggggta
     3901 tagccattct tcaagtcaaa cacctccccc aacgtcttcc aaaccacatc tttcagacgg
     3961 cctttatagc catcagctat ccccccgatt tgattgtcaa aatctaaaag aaagtcgcgg
     4021 taataccggt attggcgttt gcgcagggct aattcagctt ccagctcggt gaatttgtca
     4081 agtattttta caattttttg ttgggtttcc aggggtggga tagggattaa taattttttc
     4141 aagttactgg catttagagc aggaatacct gtaaatgtac ataaggcatg aatctcttgt
     4201 tcaaattcaa gtaatatatg atagagaaaa cgaacatcca attctgttct attcttaata
     4261 gtaactgcat aatttaagtt gccacgaaaa tatcttcctt cttgccacgt tattgagccg
     4321 actcccgctc ctcttgttgt gatgccgata gggtcatttt cagtgttcca ttcatcaata
     4381 tagccaagcg gctctttgcc actgttaata actggataac taccaatatt atttgagatt
     4441 ttctgcttac ttacagcttg tcctgttttt atgatagcta tcccattttc tccacccaac
     4501 ggcttccact ccaccggtgc cgtctgaatc atctcaatca attttttcgc tttgctttgc
     4561 atatccatgg ttgttttccg tttctttccc gctgtggcgg gatgtggggc ggttgtgggg
     4621 tgcttcggct cactctgtcc gtttgccgtt atttctgcgg gctgaagtcc gtcctgcttt
     4681 caggctgcct gcaatggtgt gcaggctgct ttttcaggta gcctgaatgg tagttatttt
     4741 gtgcggtctt ttttctgttt gatctgttgc acggctgttt tttccaacgc tttaacgctt
     4801 tccagatagg cttgctccac cggcgacagg atgcgcgctt cataggcgcg gtattcgcgt
     4861 tcggctttct gctctgcctg tttgcggctg atgctgcctg caccctgtaa tgtgccttgt
     4921 ccgtacagcc cggaaaattt gtccaattcg gctatccagt cgcgcatata catggggctt
     4981 tgctcctgcg ctttgatttc cgccaggtcg aagaaggcgg aaaccagacg gttcaggcgg
     5041 aacagttcgt cttcggtcag atagtttttg gcgattttgg cttcattcag cgtggggatt
     5101 gcgccttgaa aggtggtcag ccccataaag tctttgctgc tgtcggcacg gctgtatatc
     5161 agctcagcta cggtttgccg gctggcggca tagtgcagtt tgttttgaac ggcggcaaaa
     5221 aaggtttggc tttcgctgct tttggggttg tagtcttggc tggtggcata taaatcaagc
     5281 acttgccggt atagggcttt ttcgctgctg cggatgtcgc gaatgcggtt gagcagttct
     5341 ttccaatagt cgccgccgcc tgtgcctttc aggcgttcgt cgtctatggc aaagcctttg
     5401 gtcagatatt cgtccagccg ttcggttgcc cattggcgga attgtatgcc ccgcgcggaa
     5461 cggacgcggt agccgacggc aataatcatg ggcagggaat aatgggcgat tttgcggttt
     5521 accttgcgcc cgttttcatt ttgaactgtc aactggaagt tgacagttgc cttctcttcc
     5581 aattcttgct ctgcaagaat ggttttaatg tgtttgctga tattttgttt ggtggtttgg
     5641 tacagttctg ccatgtccgc ctgcgtcagc caaagctgtc cgccaaattc ctgcaaggca
     5701 aattgggcag tgccgtcgtt ggcggtgtat aggatgatgc tcatgcttca atctctgcac
     5761 tcacttcgtc aatttcacgc cgcagccgtt cgattttggc aacggtttcg ctgatttcgg
     5821 cgttaagctg tttgatgtcg ataatttcgc gggtgtcttc gggttcaaca tagctgctga
     5881 cggcgaggtt gtagccgttg tctttgacgg tttgctgggc ggcgttttgg gcgatatgcg
     5941 gcacatcggc tttatcggcg aagagtttga cgatttcggc aatgtgttct tcggttaaga
     6001 cgttgttgtt ggtttctttt ttaaagaagc cgcctgcgtc gatgaattgg atgtcggtat
     6061 tgtctttgtg tttggacaaa accagtatat tgacggcgat gccggtgccg taaaagagat
     6121 tgggcgcaag ggcgatgacg gtttccacgt agttgccctc caccagatat tggcggattt
     6181 tctgctctgc gccgccgcga tagaaaatgc cggggaatga gacgatggcg gcgcggcctc
     6241 tgccagaaag gtagttcagt gcgtgcagaa tgaaggcaaa atcggctttg gatttcgggg
     6301 cgagtacgcc tgcaggggca aagcggtcgt cgttgattaa ggtggggtcg ccgctgccta
     6361 tccagttgat ggaatagggc ggattggaaa cgacggcatc aaagggtttg ctgtctttga
     6421 gtttgggatt ggtcagtgtg tcgcccaatt cgatgtggaa tttgttgtaa ttgacgttgt
     6481 gcagaaacat attcatgcgg gcgaggttgt aggtggtgtg gttgatttcc tgcccgaaga
     6541 agccttcttc gatgatgtgc tcgtcaaact gttttttcgc ctgcaagagc aggctgcccg
     6601 agccgcaagc tgggtcgtag attttgttta ctttctcctg cccgtgcacc gccagccgcg
     6661 caatcagctt ggatacgctt tgcggggtga aaaattcgcc gccggatttg cctgcgttgg
     6721 cagcgtagtt ggaaatcagg tattcgtagg catcgccgaa aaggtcgatg tggtggtctt
     6781 caaaactgcc gaaatcgagt tccgccacgc cttttaggac ggcggcaagg cgtttgttct
     6841 tgtcggcaac ggtgctgccg agccggctgc tggtggtgtc gaagtcgtca aacaggcctt
     6901 taatgtcttg ttcggacgga tagccggagg cggagctttc aatcgcggta aaaatttctt
     6961 tcagcttggt gttgagctct tcgttttgat gggcttcggc ggcaatattg caaaaaagct
     7021 ggccggggta gatgaaatag cctttgactt tgacggcatc gtctttgatt tcgggcgtga
     7081 tgatgctgtc cggcatagcg gcgtaatcaa tactgctgtc gcctgcctgc atatagtcgg
     7141 taaagttttc gctgataaag cggtagaaaa gtgtgccgag aacgtattgt ttaaagtccc
     7201 agccgtccac cgcgccgcgt acttcgtcgg caattttcca aatttggcgg tgcagttggg
     7261 cgcgttgttg tatttcggtc atcatcgaaa tccatataaa agttaaacaa atcgaaatcg
     7321 cctgatattt tcagacgatt ttttacgggc attcaaatta agccaaattt tccagcatcc
     7381 atttgacgta gcgctcgacg cctgatttga catcgaaaaa ttcttccgta taacctgcct
     7441 cacgcaattt ggtgatgtcg gcttgagtga agctttggta tttgcctttg agcgcatcgg
     7501 gaaatgggat gtagcggata agttcttctt ctaccagctc tttcaagctc atttcgggtt
     7561 tgccttcggc ggcgcggcag gcgttgacgg tggcggcagc gagttcgttg aactgttggc
     7621 tgcggcctgt tccgaggttg tagatgccgg aaagttcggg atggtcgaag aagtagaggt
     7681 tgactttggc aacgtcttcg acgctgacga agtcgcgggt ttgttcgccg ttgccgtagc
     7741 cgtcgttgct gccgaacagg ttgacgtaac cgtgttcgcg gtattggtgg aagtggtgga
     7801 aggcgacgga tgccatgcga cctttgtgtt gttcgtgttg tccgtaaacg ttgaagtagc
     7861 ggaagccgac gacttgggcg gtgagacctt ctttcatgcg gcggcgcaat acttggtcaa
     7921 acaggaattt ggagtagccg tacacgttga gcggtttttc gagttcgcgc tcttcgcgga
     7981 agatttcgcc tttgccgtaa accgccgcac tggaggcata aaggaaggga atgcgttcgt
     8041 cctgacacca gtccaacaga tccagcgtgt actggtagtt gttgtccatc atatacaaac
     8101 cgtcgtggtt catcgtatcg gaacacgcgc cttgatggaa aacggcttcg atgttttgat
     8161 aaggtaaaat gtgttccctc acttggcgga tgaattcatg tttgtcgaga taatgggcga
     8221 tttcgcactc ggcaaggttt ttgaattttt cgcctttgct caaattatcg acggcaacaa
     8281 tgtcagtaat accgcgttga ttaagtgctt tgacgatgtt gctgccgata aagccggccg
     8341 cgcctgttac gatgatggtc atattcggtt tcctttgttt tgagtaaaat taataaactt
     8401 agttacaaga aatcatcgat attgtcctga aacacaaatt tgcgatgata ttccaaatat
     8461 ttttctcttc cttcgggata tattctcatc ttattttttg catttggatt gatattaagt
     8521 ttttcccatg tgtaacggct tagtcgagta ccgcaaatca aacgaccatc atccaagaaa
     8581 gtaatataac cttgattgaa caaccaatca taagtcggcg ttaaagctaa cccatttaaa
     8641 taatctaatg cctctttctc atttttctca gtaatacaaa ccatataagg tttaatatga
     8701 ctagccctta acaaaatctc atctgtaatt aatgtaaatg gacattgcgg catatgattt
     8761 ataacatcct tgcgatattt ctgctgccct tttctacttt tctgtttaat gatttcttgc
     8821 tctacgaatt gattaaattc tgaagccggt atttctaatt tttctcctga taagagttgc
     8881 gggtgcacga tagagcgaaa ttgataatcc aaaaatattc ggaaataaaa taatggttct
     8941 gaatcttcta tatctttgac aggcaatagt ttaagaattg ataaataact aattttgggc
     9001 aatacaatct tacgccaaat tgaccatata tcatcctccg aacggatata acccctacgg
     9061 taacctttag aattttccaa atggtctgat acatcatgga ttgaaaagaa aatattttct
     9121 gtaggcagat tattaatact taaaacgtaa tcatcataaa accttgaaat atcatgatga
     9181 tatttctgtt cttgtttgtg atattcaact tttgcatcaa ccaaataacg ctgtaaattt
     9241 ttcttattaa aaaaacaatt tttctgaatg agaggatatt ctaccctcct tccatttttt
     9301 gtctcagtaa tcccccaatt ttcatactca aaaaatgtac ttagttttcg accactatca
     9361 tcaatataac tacctacata tttgcgtgct tccccattac caccaaaaac agataacttg
     9421 ttattcctgt gaataaaact atcttccgca cgtaaatttt gaatactatc gacaataaaa
     9481 tactctatgt tttcaatagt aattctttga aaatccggca ttttactctc cataaagcgt
     9541 aatatccatt tgttttaata tagcgattaa tacatcaaca acaatactgt ttcccgcttg
     9601 gcgatacatt tgagtatcac taactaaaat tttaaaatca tctctaaaac ccattagccg
     9661 caaacattcc ctaggtgtta atttacgaat acgtccacga ttatgagtaa cataattatc
     9721 aaccccggca cgatgcattt tatgcatagt ttgcaataat gggcgagcta caggcaaatc
     9781 agtttccgta ctggttttaa aattttttgt tcctcctgcc aaaacataat ttttgatttt
     9841 ttcagaaaga taatattttt cctcaacgtt atttacatca aaaataaagt catcaaattc
     9901 agattcgcgg gctgcctgaa aaataaaatc accatgccaa ttaaattgtt gatttgcttt
     9961 ttggcataag gcaatttctc cattaatctg tgtataacgt ttttgtctat ttttagaact
    10021 ggtaacaaat ttcacaccct tttcacgtaa aaaatatttg ctatcagtat agtcctctaa
    10081 aaaatcttgc atagtatgtt ctaattcaat cttttcagga aactgaaaac catttatagg
    10141 aggggtatga aagccaacaa caaaaatacg ctcacgatgt tgaggaatcc cataatcctt
    10201 actattcatt atttggaaat ataagtcata accaagtgaa taaaaaacac tttttacaac
    10261 tttccaagtt tttccattat catgattgag caagcccttt acattttcat aaataaatat
    10321 ctttggttgg acttcatcca caacgcgagc aaattcatag aataatgttc ctcgtgtatc
    10381 ttctaatcct gcacgtttgc caaccatgga aaatgcttgg caaggactgc ctccaactaa
    10441 aatatcaact tgatttctaa acttgctcgc atcaaattga gtaatgtcgt tatgccaaaa
    10501 atcttcattt aatttatagt ttccaagata acttttttta acgtatggat caatatctcc
    10561 tgcaaaaaca atggtatggt ttaaatttaa tcggtggaat gcctgttcaa ccgcaccaat
    10621 cccgctgaat acggttgcta atctaatatg tgaatcaggt ttaagaaaag ttttagattt
    10681 ccaaccttgt tgactgggaa agagcaaagt tttttgtaat cgagtatcgt gtgtctgtgc
    10741 cattgtcgaa atagtcatac ttatatcgtt ctgtttatct tatcaatatg aaaactacat
    10801 tgttgattgc cctgacaatg ccttggtcaa ttcggcaaac gagcaaaccg ccgtaccgag
    10861 tttcgccacg acaaccccgg ccgcagtatt ggcaaggtac atggcttcgg gcatggtgca
    10921 gcctgccgcc aaacccaagc ccattccggc aatgacggtg tcgcccgcac cggatacgtc
    10981 gtaaacttct tgggcgcggg tgggctggta aatcggttcg ccttcactga acaaggtcat
    11041 gccttcttcg cttcgggtca gtaaaacggc ggtcaggtcg aggtggcggc gcaggttttg
    11101 cgctttttcg gtcagctcgc cttcgttttt ccaactgccg accacttctt tcaattcggc
    11161 gcggttgggc gtaatcaggg ttgcgccggc gtatttttcg taatcatcgc ctttggggtc
    11221 gattaatacg attttgcctt cgtgtttcgc acaatcgatc atatcggaga tgtgcgacag
    11281 gccgcctttg ccgtagtctg aaaaaataat tgcatcgtat tcggacaata cttcgaggta
    11341 tttctgcttg atttgttcca atacttcgcg gttgggatgt tcttcaaaat caaggcggat
    11401 aagctgctgg ttgcgggcga cgacgcgcag tttgacggtg gtggcgattt gcttgtcgcg
    11461 catcagatag gaggcgacgc cgtcttgtac catcagcgcg tcgagcgcgt cggcggcttc
    11521 gtcgtcgccg gttaccgaca acagccctgc cctgccgccc aacgaagcga tgttgcgcgc
    11581 aacgttcgcc gcaccgcccg cgcgttggtc gatacgtccg atttttgcta cgggaacagg
    11641 ggcttcgggc gaaatacggg acacatcgcc aaaccaatag cggtcgagca tcacgtcgcc
    11701 aacaaccagg actttggctt gtgcgaaacg ggatttgagg gtttcttgtt gaaacttggc
    11761 gggcatattg cctccgttgt gttttgttca gacgacctta accggtaagg ccgcctgaaa
    11821 atcagtttgc ttcaaggttt gccacgcggt tcacttcgcg caatacggct agcggattgg
    11881 cggcttgggt aacggggcgg cccatcacga gataagtcga acctgccgcc aaggcttcgg
    11941 cgggcgtcat gatgcggcgt tggtcgtcgt tgttgccggc aacgtccaag cggataccgg
    12001 gcgtgaccaa gacaaaatct tgtcccaact cgcggcgcag cggtgcggct tcttgggcgg
    12061 agcagaccac gccgtccaag cccgaacttt gcgccagttt cgccaaacgg atgacttgtt
    12121 cttcgggagc gatgttcaaa ccgatttccg ccaaatcgct ttgttccatg ctggtcaaca
    12181 cggttacgcc gattaagagc ggctttgtgc cgtatccggc aacggcttct gctgcggctt
    12241 ccatcatacg gcggccgccc gacgcgtgca tatcgaccat ccaaacgccc atgtcggcag
    12301 cgactttgca ggcttgcgcg acggtgtggg gaatatcgtg gtatttcaaa tcgaggaaaa
    12361 gtttgaaacc ttgattgatt aagctttctg ccaaactgcg tcccgtcgcg gtaaacagct
    12421 ctttgccgat tttgatttga cacaatgtcg ggtcaaggtt gcggacgaat ccgagcgtgt
    12481 ctttttcgtt ggcaaaatca agggcgacga tgacgggggt gcgttgttgc ggagtttgga
    12541 agtcggagat taaaggattc atggtgtttt cgtttcgggt tttcagacga cctttatcgt
    12601 ctttttcgca gtatagtgga ttaactttaa accggtacgg cgttgtctcg ccttagttca
    12661 aagagaacgc ttctctaagg tgctgaagca caaagtgaat cggttccgta ctatctgtac
    12721 tgcctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataaca acgtaaattc
    12781 aaaccgtagg tcgggcattg atgtccgacc tacgcggact caagcctccg acaaactgtt
    12841 tcggataaat ttgaaccggt acatcagccg tcattcccgc gcaggcggga atccattgat
    12901 aaaactcagt tcatcagatt taaacagcga ttgcccaaat tcagaaatgg attcccgcct
    12961 gcgcgggaat gacgccaaat gattattttt cggttgcaac acgctgttta tcaaaaacag
    13021 gtcgtctgaa aacctgattt tctgttttca gagtgcgaag cctgcccgtg ggtacgacct
    13081 tttcgattta tttgcccaat gcgcccaata cttcggcttt cacggcttct accggctgag
    13141 tgccgtcaac tttgatgtat ttaggcgcgt gttcgccttc cagtttgctg taaaaatcga
    13201 ccaaaacttc ggtttgctcg tggtaaacgg caaggcgttt tttcacggtt tcttctttgt
    13261 cgtcgtcgcg ctgaatcaaa tcttcgccgg ttacgtcgtc tttgccttca actttgggcg
    13321 ggttgtaggt aacgtggtaa gtacggccgg aagccaaatg cacgcggcgg ccgctcatac
    13381 ggtcgacaat cacgctgtca ggcacgtcga tttcaacgac tgcatccaaa tccacgcctg
    13441 cttcaaccat cgcttcggct tgtgccaatg tgcgcgggaa accgtcgaac aggaaaccgt
    13501 ttttgcagtc gtcttgcgcg atgcgttctt tgaccatgcc gataatgatg tcgtcgcgca
    13561 ccaagccgcc ttcgtcaatg atttttttcg cttccaaacc caagggcgtg ccagccttaa
    13621 tcgcggcgcg gagcatgtcg ccggtagaga tttgcggaat gccgaacgcg gcggtgatga
    13681 attgcgcctg agtgcctttg cccgcgcccg gcgcgcctaa aagtaatgct ttcatgaagt
    13741 gatcctttga aatgtgttta ttggaatcaa acagatggtt tacgattcta cacggcttgg
    13801 cacaatagtt caaaaaccaa ctgcacaaac tgccatttgc aagcaaatgt tttgcatacc
    13861 tttgccgagt ctgatattca caagccggca actttttggg acaatcccca aaatttcaaa
    13921 acatcacacc ctaccaaaaa ggaacatccg tgaaacgaat ttttctgttt ttggctacca
    13981 atatcgctgt tttggtcgta atcaacattg ttttggcggt tttgggcatc aacagccggg
    14041 gcggcgcggg cagcctgttg gcgtattccg ccgtcgtcgg cttcactggt tcgattattt
    14101 cgctgctgat gtccaaattc atcgccaaac aatcggtcgg cgcggaagtt atcgacacgc
    14161 cgcgcaccga agaagaagcc tggcttttga acactgtcga agcccaagcg cggcaatgga
    14221 acctgaaaac acccgaggtc gccatctacc actcccccga acccaatgcc tttgccacgg
    14281 gcgcatcgag aaacagctcc ctgatcgccg tcagcaccgg tttgctcgac catatgacgc
    14341 gcgacgaagt ggaagccgta ttggcgcacg aaatggcaca cgtcggcaac ggcgatatgg
    14401 ttacgctgac gctgattcaa ggcgtggtca atacctttgt cgtgttcctg tcgcgcatta
    14461 ttgccaacct gattgcccga aacaacgacg gcagccagtc ccagggaact tatttcctgg
    14521 tcagcatggt attccaaatc ctgttcggct tccttgccag cttaattgtc atgtggttca
    14581 gccgacaacg cgaataccgc gccgatgcgg gcgcggcaaa actggtcggc gcgccgaaaa
    14641 tgatttccgc cctgcaaagg ctcaaaggca acccggtcga tttgcccgaa gaaatgaacg
    14701 caatgggcat cgccggagat acgcgcgact ccctgctcag cacccaccct tcgctggaca
    14761 accgaatcgc ccgcctcaaa tcgctttaaa ccgatttgaa acggcaaaaa ccgtacgcgc
    14821 aagcagtacg gttttttgta tatgctgtct gaaaacatca ggtttccata ccgacaccaa
    14881 ccgtatccat ttccgcaccg gatacgccac attgatatac cctgataaac cgtatccgga
    14941 cagaaaatat agtggattaa caaaaaccag tacggcgttg cctcgcctta gctcaaagaa
    15001 aacgattctc taaggtgctg aagcaccaag tgaatcggtt ccgtactatt tatactgtct
    15061 gcggcttcgt cgccttgtcc tgatttttgt taatccccta tatcagataa aacagcggta
    15121 aaattgccgt ctgaaaccaa ctctgcgcca tacgggacac gctctgcacg tctatttgcc
    15181 tccgatatat cctccctact gtaggaacgt ctgccgattt gcacgaaatc ggcacctcta
    15241 gcacccttca ggatggcgga cgttatccgt tcttgggatt ctgcaagacg gtcgacggca
    15301 ttatacagac gctcggtctg atacccttcc gaagcaacct gcttcaggta ttccaaataa
    15361 ccgtaaccca acgcccctcc gacaagcagg gtcgtcaacg ctaccagcgt tgccgaacgg
    15421 attttagggg gcatatcttg aagcatatct ctaccgaacc gaaccaaagt ctctttaaag
    15481 tcggactcta cacgcgtaga accctcggaa atcttgaagt tcaactcata ctgcttccgt
    15541 ttgtccgctg tcaaccgctt gaggttgtca tccccgtatt ggacggttga cacaaccctg
    15601 tacagactgt cctgtatttc gaccagcgtc tgtgcaaagt tggcggtcat aactggattc
    15661 ccaacccttg ccgccgattc tgatactcaa aacggtatcc aggcgttcca gcagggcttc
    15721 gggagacagc agtgcgtatt cttcaatatt ttccataggt tgattatagc agaaccttat
    15781 acctgttaca gacaaggttg tcgggatttg aacgtccgtc gagcttggtt cgtatagagc
    15841 cgtagggggt gtttagggct ttggcggcag cttgtacgct ggggaacgtg agtgtttcgc
    15901 ctgtggcggt gtcgaggatg gagacaggtt tttggttttg gggaggcgta ttttccttca
    15961 ttttaagttt cccgcactcc ggacacccat actttgactt tatagctgtg tcgtaataca
    16021 aaagttggac aaccccgtgc gtaggacatc taacactgga aggtttataa atgccactga
    16081 actccaacaa ttctaaatgt ggaaatttct cttgaaagcg tgatttggcg gtatcgaaag
    16141 tcattcgctt acccataatt tgtctccata ggttatgtgc aaatggatta cacaaccaaa
    16201 atatagaaat tttcaaattt tttgagacct acctgaaatc ctgcgcaagc acaatgtaaa
    16261 gaaaaatggt atacgtcttt actaaatctt tacataaatt aactctaacc gggatttgaa
    16321 cgtccgtcga gcttggttcg taggatttag ggtgttgtga accttaccta tcgtacccaa
    16381 gcactttgta cctgtttgaa ataaggttgt cgggcgacgt tcgacccttg agcctgtgat
    16441 tgatttgttg gaaatgtaca cctaaagcag cacctgcggc agtaacgctt gggaacgtga
    16501 gtgtttcgtt ggtcgttgtg tcgaggatga ggaggggctt ccctacattt ggaggtgttt
    16561 gggtttttga catcttagca cactcaggac acccgtactt tgactttata agggcattaa
    16621 atgtcgaata acgaacgttc ccgtgcatag gacaattaac ggttactggg taattcacac
    16681 cgtcaaagcc ggttagagag atattaggga atatatcagt aagttcagaa gctgcttggt
    16741 tgaaagattt ggccatttga gactccaaaa ttgattgtca aatggattta atagcaaatt
    16801 tataaaaatg tcaaattttt cgagaagcac ctgatagtgg cgaggggtat acactgttaa
    16861 aaagtgtata cgtcatttag tgcagatgtg aaccttatac ctccctgcaa gtaaagtctc
    16921 tgtgtgaacc cgacctttta tacggtcagt gatagctgct gggtttatac ccaatgacct
    16981 tgcagcagat gctcttgagg ggaatgtaag tgtttcgcca gttacagtat cctccaatac
    17041 tgtattttta gataatttat ctattgtatc ttctggaatt ttgtgggaat tgacaccata
    17101 agatgcacat tttggacacc cacttttaga gtccaacata cttttgaatg tagagcaagt
    17161 aaccctcccg tgtataggac agacgattac cgtcgggtaa cgaacgccat tgaactccac
    17221 aagattcaaa tgtggaaatt tagaggttag cttagaaact gcttgagtaa atgtaagtgc
    17281 catagtggtt gtgaacttac tttaccaacc tataacgata cctaatcaat cggtcaaacg
    17341 gagtgcgtcc gcttaaacgt acagctaaag tgctgttgga gcaaccgata aatttactgg
    17401 cagccagtgt acttgcaaac tcgtagattt cccctgtctc tgtgtcctcc aacctaatgg
    17461 caacggcgtt tggagagggc ttttcgggat ttcgagtaca ttcaggacat cctgttgagg
    17521 atttcatggc agcttgtaga ttcccgtaaa caaccgaccc atgcaaaggg cattggattg
    17581 ttaccggttc gtagttttta gtgaacttca aaaccgtctt atcaggaaat ttactggaaa
    17641 accttaactg aacatcttgt agcgagagtg attttggcat tatagacctt tctaaaatga
    17701 aagcattata caaccaaaat atagaaattt tcaaattttc tctgaacgca ctggtatccg
    17761 atggagctaa atgtaaagaa aagggtatat gtccttacta aatctttaca cattttaact
    17821 gtatagtgtt tgacaaacaa caggcatagg cgcataatgc acccgtagtt agatagatag
    17881 taaggagata accaatgact tagcacctgc ccccctctct ctcgcgcacc ccgcccctcg
    17941 cttgcggcta atgggctttg cgcgaaatat acactttcat ctcctgcatg gcttaaccgc
    18001 ctatgcaata gtacacacat caaccaatca ggctggcacg tcttggcatt ggctgacgta
    18061 ccattgtagc ccgaaatgtt gataccctac tatttatgac caacggcgga ttagggcttc
    18121 agacggcaat tttactgcaa aaccttatgc gaacggatga tgcagcacaa tcgtttcttc
    18181 gcggtcgggg ccggtggaga cgatggcgac cggcgcgccg cagacttctt caatccgttt
    18241 caaatatgct ttggcattat cgggcagttc ctcataattt ttgatgccga aagtcgattc
    18301 tttccaaccc tccattgttt cgtaaatcgg tttgcaggtt tccaccgcat ccgaaccgca
    18361 aggcaggata tcggttttgc cgccgtcggg caattcgtag ccgacgcaga tattgatggt
    18421 ttcaacgccg tccattacat cgagtttggt aatgcacata ccggaaatgc cgttgatttg
    18481 gatggagcgt ttcagggcgg cggcatcaaa ccagccgcag cggcgtgcgc ggccggttac
    18541 cgagccgaat tcgtgtccgc gttccgccaa acctgcgcct acttcgtcga acaattcggt
    18601 cgggaacggg cctgaaccga cgcgcgtggt ataggctttg acgatgccca aaacataatc
    18661 cagcatttga ggacctacgc ccgcgcctgc ggaagcggct cccgccagac agttggacga
    18721 ggtaacgaag ggataagtgc cgtagtcgat gtccaacaac gcaccttgcg cgccttcaaa
    18781 cagcagtttt tcgccgtttt tgtttttctc gttcaacacg cgggacacgt cggtaatcat
    18841 cggcgcaatg cgcggcgcga ctttttcgat aaccgccatc acgtcttccg ctttaaccgg
    18901 ctcggcattg tgcagatgtt gcagttggac attgtaatag gcaaggacgg catccagttt
    18961 ttcacgcagt ttttcaggat gcagcaaatc ggcggcgcga atggcgcggc gtgccacttt
    19021 gtcttcgtag gcagggccga tgccgcggcc ggtcgtgccg attttgcctt tgccgcgcga
    19081 tgcttcgcgg gcttggtcga gcgcgatgtg ataaggcagg atcagcgggc aggtcggcgc
    19141 gattttcaga cggccttcga cgtttttcac gcctgccgcg ttcaactcgt cgatttcgcc
    19201 caacagggct tcgggggaga cgacaacgcc cgaaccgatg aagcagtcca aactttcatg
    19261 caggatgccg ctcggaatca ggcgcaaaat ggtttttttg ccgccgacaa ccaaggtatg
    19321 gcccgcattg tggccgcctt ggaagcgcac cacgccgccg gcttcttccg ccagccagtc
    19381 aacgatttta cctttaccct cgtcgcccca ctgtgcgccg attactacaa catttttagc
    19441 catagccata taacctatcg atattaaaaa ttattgcgga aatccccaaa ccggtgcggc
    19501 tttgcgcccc gccacgattt gcctttcatc cgccatgaac gggtatttag cgtttcacga
    19561 cctgccaaac gccgtccgtc tttttcagac ggcctgcaag ctcttccgaa acattgtgtc
    19621 cgataccgta atcgattacg acacactgcc cttgttcacg caaggcttcg accgcttcgc
    19681 gcgccgcttc ggcatcttcc gcatcgacca acacggcggg ctgccgttcg atggcgggca
    19741 aacgcccgat aaagctgcgc aagtcgaaac tgaatcccgt tgccgggcgc gccctgccga
    19801 aatatccgcc caatccgtca taacgcccgc cgcgcgcgac cgcgtcgtgg aaatcggcgg
    19861 cataggcggc atacagcaag cccgtgtggt aattgtcgac acgcagctcg gacaagtcga
    19921 tatggatttc acaatcgggg aatgcgtcgc acaccgcctg caattcgccc aacgcgccgc
    19981 cgaccgccga caaatccggc aaccgtccgc gcgcgtcgga caacacttca cgcccgccgt
    20041 acaggcgcgg caacagcgag aatgcttttg cccacatgcc gtccagcttc caagccttga
    20101 cctgcgcttc gaccgccccg gtatctttat cctgcatcaa ggcaagcagc gttgcggact
    20161 gccccgcatc caaatgcgcc gcatcggaca aggcgcggaa tatgccgata tgccccagcg
    20221 aaagcagcac tttgcccata tcggcaattt tcatgctttt cagcatcagg tctatcagct
    20281 cgatgtcgcc acggatgtcg gcaaaaccgt acatttccgc ccctgcctgc aagggttcgc
    20341 gcatattcag cagaccgtcg ggctgcgcgt gcaacaccgg accggcataa cacaaccggt
    20401 taatcccttg gttggcggat aaaagatggg catcaatacg cgccacctgc ggcgtgatgt
    20461 cggcgcgtat gcccaactgc ctgccgctga gcctgtccgt taccaaaatg gttttcaggg
    20521 aaagccccgc atcgatatgc gtcagcaggg aatgtgcgta ctccatcagc ggaggctgta
    20581 ccagttcata accgtgtacg cggaacagtg ccaacaactg ctccctcgcg ctttcaagct
    20641 gccgcgcgtt cgtgggcagt acgtcggcga tatgttccgg aagctgccat gtctgcatga
    20701 gaatctgcct tctattttat catgtaaata caaaaggctg cccgatccgc aatcagacgg
    20761 catctttgcc gcaacgcatc acgccgctgt caaaacggcg atgcttcctt ccgattcaga
    20821 atccgaacag cccctgtccg acggctcaat gccgtcaagt cgttaaaacc aaactttacc
    20881 ataaaataca cacaatctga atatactttt ataccctgta tccgccgtct gacggcacgg
    20941 cattttttta ctatataatg tgcgccatta tttctcatca cttcctgctg ccgttttctg
    21001 cttcagacgg catttttgtt gattgaacac ttatgctgct cgacctcaac cgcttttcct
    21061 tttccgtctt cctgaaagaa gtccgcctgc tgaccgctct tgccctgccc atgctgttgg
    21121 cgcaggtcgc gcaggtgggc atcggttttg tcgataccgt gatggcgggc ggtgcgggca
    21181 aggaagactt ggcggcggtg gctttgggca gcagcgcgtt tgccacggtt tatattacct
    21241 ttatgggcat tatggcggcg ctgaacccga tgattgccca gctttacggc gcgggtaaaa
    21301 ccgacgaagt gggcgaaacg ggacggcagg ggatttggtt cgggctgttt ttgggcgtgt
    21361 tcggcatggt cttgatgtgg gcggcgatta cgccgttccg caactggctg accttgagcg
    21421 attatgtgga aggcacaatg gcgcagtata tgctgttcac cagcttggcg atgccggcgg
    21481 caatggtaca ccgcgcactg cacgcctacg cctccagcct gaaccgcccg cgcctgatta
    21541 tgttggtcag ctttgcggcg tttgtgttga acgtgccgct gaactatatt ttcgtttacg
    21601 gcaaattcgg tatgcccgct ttgggcggcg caggctgcgg actggcgacg atggcggtgt
    21661 tttggttcag cgcgctggca ttgtggattt atatcgccaa ggaaaatttc ttccgcccat
    21721 tcggactgac ggcgaaattc ggcaaaccgg attgggcggt gttcaaacag atttggaaaa
    21781 tcggcgcacc catcgggctg tcttattttt tggaagccag cgcgttttcg tttatcgtgt
    21841 ttttgattgc gcctttcggc gaggattatg tggcggcgca gcaggtcggc atcagtttgt
    21901 cggggattct ctatatgatt ccgcaaagcg tcggctcggc ggggacggtg cgcatcggct
    21961 tttcgcttgg gcggcgcgaa ttttcgcggg cgcgttatat ttcgggcgtg tcactggtgt
    22021 taggatggat gctcgccgtg attaccgtgc tttccttggt attattccgt tcgccgctgg
    22081 taagtatgta caacaatgat ccggcggttt taagcatcgc cgccaccgtc ttactgttcg
    22141 ccggcttgtt ccaaccggca gacttcaccc aatgtatcgc ctcctacgcc ttgcgcggct
    22201 acaaagttac aaaggtgccg atgttcatcc acgccgccgc cttttggggc tgcggtctgc
    22261 tgccgggcta cctgctcgcc taccgtttcg atatgggcat ttacggcttc tggacggcat
    22321 tgattgcctc gctcaccatc gccgccatcg ccttggtgtg gtgcttggaa ttgtgcagta
    22381 gggagatggt cagatcgcat aaggccgtct gaaaactcca tgggtaggat gaagtctgct
    22441 aattacaata ttaatagatg atgtggcagg gttgagctag gagaatttag agtttttgaa
    22501 gggagagagt ttggtgtttt agcttaatct ctatttctaa cttaagataa ggaaaccgtt
    22561 atgtttataa aaagtatttg tttatctaat tttaaagggt ttatcggtga taaccatcag
    22621 attaaattta aaattcctga tggagcaact gctggtagtg gtttgaacat ttttatcgga
    22681 gaaaataact ctgggaaatc gtctattttt gaagctataa atttcttaag gaacggtatc
    22741 aaagaagatg aagcttatag aataaaaagt aaactggcag atgatactca gccaaatgac
    22801 gcatgcgttg agctggcttt ttgtgggaat attcaaaatg caataactta ttttgttcaa
    22861 aggaataaac aagcagcttt taataatgca attaataatg agcagaattt aaaagctaag
    22921 agaaatacag tttcttgtaa gaaattggat ttgtgggata acgaatctca ggactattca
    22981 aatccaagtg ggatcgatgc tccttttaag gctctttttg aaactaattt tatttgggca
    23041 gatacaaatc ctaatgatga agcggctttc gggtctacaa ctctttgcgg attgttactg
    23101 aaagaaattg ctcaggcgca tatcaattcg gctgaatatc aaacatttcg tgatagtttt
    23161 aatcaggtat ttaattcacc agcttctaat ttgcgtcaac aaattgctac tatcgaacag
    23221 aaagttcaac gtatttttac tgaacaattc ggacaagctg atattcattt tagatttgat
    23281 gaactaaaga tagattcgtt ttttaaaaat gcctctatct ttattgacga tggtgtggat
    23341 attccaatgt ccgaaaaagg taatggtatg cagcgttctg ttgcattagc cttgcttcaa
    23401 gtctatgcag aagagttagc gcatgatgaa gagcaaggtc agactaagcc gttttatttg
    23461 tttattgatg agcctgagct ttgtttacat ccaaaagggc aaactaaatt acttgaggcg
    23521 ttgttagaaa tttcaagaac gaaacaagta tttttgacta cgcattcccc atattttttg
    23581 gttacacctc atttaagtaa tgttggtctg tttattttta gaaaggacgg aattagcaat
    23641 attgttgaag acgcttcatt ggagccaatg tttccttgga gcccaacttg gggagagatt
    23701 aatttcaagg cgtataaatt acctaccgtg gagttacata atgaattata tgggaaatta
    23761 caacatgatt gtgggcaatt tcgagaaaaa gactttgaac aatggttaca gagtaggaat
    23821 attcagttca ctaaacaatg gatacgagaa aacaatggat ccgcacaagc tccatatgac
    23881 gtaaccttgc aaacatttat ccgcaaccat attcatcatc ctgaaaatcg gcgaaatgca
    23941 ctttatacgg aaaatgaact tagacaatct attgaggaaa tgattagatt gttatagttg
    24001 aaggagtaaa aagtgcggtc aattctctca atgttttcaa aacgtcgtct gaagccacac
    24061 tttctagccc aaagaagaaa attcagttat tcaaaatttt tgggtaaccg ttgatgatgg
    24121 taaaaactaa aaaactttga tcgcctgctg aaataaggac aatttgagga taaacagcaa
    24181 taaacctgtt taatctttgt tttgataaaa acatatggag ctgtcctaga taactaggat
    24241 aaactcgatt ttactaattg ttttaaaatg gaacaagaac ttttatctca ctgttgttaa
    24301 aacgccgttc gtaccccttt aaatacagct caaaatgcgc tttgggaatg ccgttaaact
    24361 tgcgtaaatg acgttttgcc tggttccaaa agttctcaat tccattaata tggttttgtc
    24421 gttcagcaaa ataactttca tctgcttcta cttcgccatc aaacatttcc aaatgtggac
    24481 tgttttgata aataagtaat cgtaaacgat gaaaataata ggctgcggta cttttattaa
    24541 cgcctactaa ctctgctgct gttcttgcag ttacacctgc gacaaatagc tcaatgagtt
    24601 tattttgttt ataccggctt agacgacttt ttctcatagg ggcaactcta acttaatttg
    24661 aatttcccta gttatctagg acagccccaa acatatttta aagctcaaac catgcaaccc
    24721 atccgatacc gaaccgacct caccccctac aacaccttcg gtcttcgcgc ccaagcccga
    24781 gcctttattg cgcttgaaca cgccgacgag ttgtgcgaca tcgtccgact gcccgagttc
    24841 gaccgcgata ctgttttatg gctgggcggc ggcagcaaca tccttttgat gcaggattac
    24901 gccggactgg tcgtacacat ggaaaacaaa ggcatacgcg agattgcgcg ttcagacggc
    24961 attgttcgca tcgaagcgca ggcgggcgaa atctggcacg attttgtcct gcacaccgtc
    25021 gcgctgggct tgaatgggct ggaaaacctg agcctgattc cgggtacggt cggcgcgtcg
    25081 cccgtgcaga acatcggcgc atacggcgta gaggcgaaag atgtgattca cagcgtgcgc
    25141 tgcttcgatt tggatacgga aacctttgta accctttcca atgccgactg ccgcttcgcc
    25201 taccgcgaaa gcctgttcaa gcaggaaggt aaagggcgtt atgtgattgt ttcggtcgta
    25261 tttgcattaa aaacgcattt tgtgccgaac ttgggttacg gcgatttggc ggccgccgtt
    25321 gccgaactga gcgcgggcag ggtgccgacg gcgaaagatg tttccgatgc agtgtgtgca
    25381 atccgcaaca gtaaacttcc taatcctaac gtgcttggca atgtcggcag tttctttaaa
    25441 aatcccgtca tcggcgcgga aaaagctgcc gccctgttgg agcagcatcc cgatatgccg
    25501 cattatccgc agcccgacgg ttcggtcaaa ctcgccgccg gctggctgat cgaccaatgt
    25561 cgtctgaaag gctttcaaat cggcggcgcg gcggtacatg gcaggcaggc tttggtctta
    25621 gtgaacaaaa acaacgcctc ggcaaacgat gtccggcaat tggcgcaaca catcaaattt
    25681 acagtatttg ctcggtttca ggtagaattg cacgccgaac ccaattggct gcctgcttcg
    25741 ttcagcctgt aaatccaagg agtatgcccg tgacccgccc cgccaaaatc aatacttaca
    25801 cacgcatcat cgacgccagc cttgcgctct tcaacgagga aggcgagcgc aacatcagca
    25861 ccaaccatat tgccgcccac ttgggcatca gtccgggcaa cctctattac cacttccgca
    25921 acaaagacga aatcatcgtc caactgttca aacgttacag cgaagccctg ctggaatacc
    25981 tcaacgaagc cgtgttgccg tctgatgtgg aagactccat caactatatg gcgggcattt
    26041 acgacgtgat gtgggaatac cgcttcctat tcagcgacgt gaacaccctg cttgcacgca
    26101 gtgccgaact attgggcgaa cacaatacct tcacccaagc caaagtctcc ccgctcttgg
    26161 tcaacctgct cacccagctc aacggtctga acatcatcca agccgaccaa accgctatga
    26221 acgacctcgc cgtcaatatg tggatggtca cgaaatactg gttcgacttc gacagctccc
    26281 tgcgcggccg caccaagctg accgaagact ccaaagcacg cggcatccgc cgcaccttaa
    26341 gcctcctgcg cccttatctt ttgcccgaac accgcgcgga atacgaccgg aaaatcggca
    26401 acggcaacct gtaaacccat aatgtccgaa tcagtcttca gacggcactt acccgacggt
    26461 atcagcgaat gcgcgcagtc cccccgcccc atatctccat cacaggccta ggttacctcg
    26521 gcctgccgct ggcacaaaag ttttaccaac acggcagccg cgtttccgcc gtcaaacgca
    26581 gcctgacttc ggacgacatc aacctgccca tacacctcga caccatcgac ctcggcagca
    26641 ccgatgcctt tcagacggca ttgtggcggc accacgccga caaacccgtc tggttcttcc
    26701 tgctgccgcc atcctcactg acccattacg ccgacaccgt caaacaatgg gcagaacttg
    26761 cccggacgtg taacgtgcaa cacctgattt tcacaagcag caccagcgtt tacggcgata
    26821 cagcgcgcga atgcgacgaa accgccacac ccgatccgca aaccgagtcc gcccgccaaa
    26881 tcctcgccgc cgaacaacac ctgctcgaca gcggcgtgcc gaacatcgac atcctgcggc
    26941 tgggcgggct ttattgcgcc gaacgccatc ccgtcggccg ccttgttcaa aagcaaaaca
    27001 tcccgggcgg caaccgcccc atcaacatcg tccaccgtaa tatcgccgtc gaaagcctgt
    27061 ttcagacggc atttaacccc ggcggcaggc ggctgaaaaa cattatcgaa ccgcgccacc
    27121 cgacacgacg cgaattctat acggaagaag ccgccaaact cggcttgccg gctcctgatt
    27181 tcgcacccga cgacagcgtg ggcaaaatca tccgtaccgt ttgcgataac ggcttaagcc
    27241 tgtaaaataa gcgtcaacag caacaaacag gcattctccg ccccaatcag aaaatatgtc
    27301 cgccctcctc cccatcatca accgcctgat tctgcaaagc ccggacagcc gctcggaact
    27361 tgccgccttt gcaggcaaaa cactgaccct gaacattgcc gggctgaaac tggcgggacg
    27421 catcacggaa gacggtttgc ttgcggcggg aaacggcttt gcagacaccg aaatcacctt
    27481 ccgcaacagc gcgatacaaa aaatcctcca aggcggcgaa cccggggcgg gcgacatcgg
    27541 gctcgaaggc gacctcatcc tcggcatcgc ggtactgtcc ctgctcggca gcctgcgttc
    27601 ccgcgcatcg gacgaattgg cacggatttt cggcacgcag gcagacatcg gcagccgtgc
    27661 cgccgacatc ggacacggca tcaaacaaat cggcaggaac atcgccgaac aaatcggcag
    27721 attttcccgc gaacccgagt ccgcaaacat cggcaacgaa gcccttgccg actgcctcga
    27781 cgaaataagc agactgcgcg acggcgtgga acgcctcaac gaacgcctcg accggctcga
    27841 acgcgacatt tggatagact aaccttcaga cggcatccga catgaacagc ccttttcaca
    27901 acatcggcat cgtaacccgc cccaacacgc ccgacatcca agacaccgca cacacgctga
    27961 ttaccttttt gaagcagcac ggctttaccg tctatctcga cgaagtcggc ataaaggaag
    28021 gctgcatcta tacccaagac accgtcggct gccatatcgt caacaagacc gaactggggc
    28081 aatactgcga cctggtcgcc gttttaggcg gagacggcac ctttctctcc gtcgcccgcg
    28141 aaatcgccct gcgcgccgtt ccgattatcg gcatcaacca agggcatttg ggcttcctga
    28201 cccaaattcc ccgcgaatat atgacggaca agctattgcc cgttttagaa gggaaatacc
    28261 ttgccgaaga gcgcatcctg attgaggccg cactcatccg cgaaggcaaa accgccgaac
    28321 gcgccatcgc cctcaacgat gccgtcctct cccgtggcgg tgccggacag atgattgagt
    28381 ttgaagtctt cgtcaatcgg gaattcgtct atacccagcg ttcggacggg ctgattgtct
    28441 ccacccccac cggatcgacc gcctattcgc ttgccgccgg cggccccatc atgcaggcag
    28501 gtttgcacgc cttcacgctc gtccccatct gcccgcaatc catgaccaac cgccccatcg
    28561 ccattccaga cacgtccgaa atcgaaatcc tcgttaccca aggcggcgac gcgcgcgtcc
    28621 atttcgacgg tcaaacccat atcgacgtgc aaaacctcga ccgcatcacc atccgccgct
    28681 accgaaaccc cttacgcatc ctacatccga ccgactacca atatttcaaa accctgcgcc
    28741 aaaaactgca ctggggcgaa caattggtct gaaccgaccc ttaccggaaa acacctatga
    28801 atccaaaaaa cccccccgac catacagaag atgccgtgcg cctctccaaa cgcatggcgc
    28861 aactggggct ttgttcgcgc cgcgaagccg acggctacat cggacagggc tgggtaacgg
    28921 tcaacggcaa aaccgccgta ctcggtcaga aagtttcacc ggccgaccgt atcgaactaa
    28981 acaagaaagc ccacgaacag caggcggcac gcattaccat cctgttgaac aaacctgtcg
    29041 gctatgtcag cgcacaagcg gaaaaaggct ataaatccgc cgccgaactg attacccctg
    29101 aaaaccgctg ggaaggcgac accggacgca tccgtttcga tccgaaacac aaaatcggtc
    29161 ttgcccccgc cggcagactc gatatcgatt cagtcggatt gctggtgttg acgcaagatg
    29221 gccgtatcgc caagcagctt atcggcgaaa acagcggcag tgaaaaagaa tatttggtgc
    29281 gcgtgcgcgg caaattggac gaaaaaggac ttgccttact gaatcacgga ttgagtttgg
    29341 acggcgagaa actgcgtccc gccaaagtag aatggcaaaa cgaagaccaa ctgcgcttcg
    29401 tgttgaaaca gggtaaaaag cggcaaatcc gccgtatgtg cgaactggtc ggactgcgcg
    29461 tcgtcgggct gaaacgcatc cgcatgggca aggtcaaact cggcaggctg ccgcccggca
    29521 aatggcgtta tctcgccccc ggtgaatcgt tttaaataag cattaaaaaa tgccgtctga
    29581 aaatccttgc gttttcagac ggcattgcct tatgcccaaa taacggtttc ttccaagggc
    29641 ctgcgcgcct tcgtggcgat ttcctgaacg cggtaaccga atgtcgcggc gacggacacg
    29701 ccccattctg ccgcatcgaa caaaccgaac tgcccggaca atacgcgttc catatcggca
    29761 tagttgaaac cttccaccgg gcaggaatcg atacctgcca tcgccgcacc cgtcatcatg
    29821 ttgcccaacg cgatataggt ctggcggcaa caccagtcaa acaaggcgcg cgaatcgtcc
    29881 aaaatcttga tgtcgtcggc ttgaaacgcc tgatacctcg ccaaagattt tgccatggca
    29941 tccggttcgg taacgccgcg ccgtttgagg ctttccaaca taaacgggct gtcgaagcgg
    30001 gcatttttct ttgccaaaaa caccaccaaa tgactggcgg tatccaaagc atccgccata
    30061 ccccaagaaa acggcttgat tgcctgtcgg atttcggggt tttgaaccac aacaaactgc
    30121 caaggctccg aaccgaccga actgggcgac aaacgcccaa gttctaaaat aaactgaaaa
    30181 tcctcggcac tgattttgcg tgccgcatcg taatgccggc acgacttgcg gtttttaaat
    30241 gcggatagga cctgctcttt gcttaatact gtcatcgtgc atccttttat tactttgatt
    30301 gcaaattgat ttcaaaccga tacggctctg ccgcaccata caatccgtcc tcgccatcaa
    30361 gcttcatccg gactttaccc atctctgcta cggctgggaa aacaaagcgg cggcagactc
    30421 gggaataacg ccccaatgcc tttcagacgg catttgggca tcattgcaaa cgcgcctacg
    30481 ggtaggcgat ataggcataa gccgaatggt tgtggataga ctcgaaattc tcgctctcga
    30541 caacgaaact cttgatgcgt ttgtcggcga tcagcgaagt agcgacatcg cgcaccatat
    30601 cttccacgaa tttcgggttt tcgtaggcct tttcggtaac gtatttttca tcggggcgtt
    30661 tgagcaggcc gtagagttgg cagctcgcct gcgcctccac ataatcgatg acttcctcga
    30721 taccgacttc ggcatcggca gtcaggctga cggtaacgtg cgaacgctgg ttgtgcgcgc
    30781 catattggga aatttctttg gaacacgggc aaagcgaggt tacgggaatc atgaccttca
    30841 tactgtggcc gtatgcaccg tctttcattt cgcccgtgag gcagacatca taatccagta
    30901 aagactgaat accggaaacc ggggcggttt tcttgcggaa aaatgggaaa gaaacgctga
    30961 ttttaccggc gcgggaatcc aaaagcgcga ccatttcggc agtcagccta tgcaattgtg
    31021 cgaaatccaa ggcttcagaa tattgctcca tcaatgcgac aaagcgcgac atatgcgtac
    31081 ccttctgctc ggcgggcagg taaaccgtca tggtcagacg ggcaatggtg gactggatgc
    31141 cttctgcagt ttgcagggta atcggaaagc gcaggtcttt gataccgacc tgattgatcg
    31201 gcagattgcg caaatctcgg ctggattgca cgtctgcaat agtgttcatg agttgttttt
    31261 ccttaatatt catgtaattg ttgagatgtg tgcatggtat ggcgtttccg ccggggaata
    31321 taccgtcaat ctgcaaatgc gagattatat cactcgcttt tagcagctgc attgattgtt
    31381 tctatttttc agatagtgat aaaatcttcg attattcaca tctacatagg cagagaccaa
    31441 atgaaattcg ccacgaaagc cattcattcc agctacgatt gcgacgaaca caaccgcgcg
    31501 ctgatgccgc cgatttatca aaacagtatg tttgcgttgc acgagattgg cgaaaatgtg
    31561 ccttaccgtt attcgcgcct gagcaacccg acccgtcaga ttttggaaga caccgttgcc
    31621 gatttggaac acggtgcgac agggtttgcg ttttccagcg gtatggcggg gattgatgcc
    31681 gtatggcgta ccttcctgca acagggcgac accatcgtcg cggtcgccga tatttacggc
    31741 ggcgcgtatg acttattggt cgatgtttat caaaaatggg gggtgaacgt tgtttttgcc
    31801 gatttgggca atcccgataa tctggacgag ctgctcaaag cgcacaaggt caaactggtc
    31861 tggctggaaa cgccgtccaa tccgctttta cgcttggtag acatcaaagc ccttgccgcg
    31921 aaagccaaag cgaccggcgc actggtcggt atcgacaaca cctttgccac gccgtatctg
    31981 caacagccct tggatatggg ttgcgatttt gtattccatt ccgctaccaa atatttgtgc
    32041 ggccattccg acgtattgat gggcatcgtc gttgccaaaa ccaaagaact ggcgcagcct
    32101 ttgcacgata tgatggtgca taccggcgcg gttgccggcc cgctggactg ctggctggtg
    32161 ttgcgcggca tcaaaacgct tgccctgcgt atggaggcac actgcaaaaa cgcactcgaa
    32221 atcgcgcgcc gtctggaaga ccatcctgcc attgaaaaag tgttccatcc cggcctgccg
    32281 tcgcacgaac attatgaact cgcccaagcg caaatgccta aaggcatcgg cggcgtggtg
    32341 acggtttatc tcaaaaacga cacgcgcgaa gcggcaaaca gcgtgattaa aaacatgaaa
    32401 ctggtcaaaa tggcttccag cctcggcggc gtggaaagtt tggtcaacca ttgctattcc
    32461 cagtcccaca gcggcgtgcc gcataatgtg aaaatggaaa tgggcatcag ggtcggtctg
    32521 ctgcgtttct ctgtcggtat cgaagatgta gatgatattt ggaatgatat ttccgccgca
    32581 ctggatacga ctgtataatc cacgaaaaat gccgtctgaa accatggttt cagacggcat
    32641 ttcaatcaaa ccgaccgaaa atcaacgctt cagcatcttt gccgcctcaa tggcataata
    32701 ggtcaaaatc ccgtccgcgc ccgcacgttt gaatgccagc aggctttcca agaccacctt
    32761 atcgccgtcc agccagccgt tggcaaccgc cgcctgcagc atcgcatatt cgcccgaaac
    32821 ctgataggca taagtcggca caccgaactc gtccttcacg cgtcggacaa cgtccaaata
    32881 cggcaaaccg ggcttgacca tcaccatatc cgcaccttcc tgaatgtcca acgccacctc
    32941 atgcagcgcc tcatcggtat ttgcgggatc catctgatag gtctttttat ctgccttgcc
    33001 caaattgccc gaactgccta ccgcatcacg gaaagggccg taaaatgcag aagcatattt
    33061 ggcggaatac gccataatcc gcgtatggat atgcccggca tcctccaacg cctcgcgaat
    33121 cgcaccgata cgcccgtcca tcatatcgga aggggcaacc acctgcgcgc ccgcttcagc
    33181 gtggcacaaa gcctgcttga ccaaaacctc tacggtttca tcgttcatca cataaccgtt
    33241 ttcgtccgtc agcccgtcct gaccgtgaac cgtataagga tcgagcgcga catccgtcat
    33301 aatgcccagt tcgggaaacc tctcgcgcaa ggcgcggacg gctgccggca cgagtccttc
    33361 agggttatac gcctcctgcg cacgctcggt tttgtttgcc gtaaccacgg ggaacagtgc
    33421 caacatcgga ataccgagct ttaccgcctc ttccgccgta aacagcagcc tgtccaaact
    33481 ttgacgcttc acacccggca tagaaggcac atcctcctcg cgcgccgacc cctccaatac
    33541 gaacaccgga taaatcaaat catcggcggt cagcgtgtgt tcgcgcatca ggcggcgtga
    33601 aaaatcgtcc ctgcgcatac ggcgcatacg cgaagccgga acattgcggt aaggaaactg
    33661 cataagccct ccaatcatcc tgtcaacaat tcaaaccata cggaagccgc cgcaacttgc
    33721 agacagcatc gggcggcaat caaaatccgt tttcaccgga aagggtttcg gcccgccgaa
    33781 atgccgcgcc gaaggacggc gtcccgccca ttaaagaaca atagcaaaac aacagcttca
    33841 ggctgtcagc cttcgatatt cttacgccac aataccaaaa gtttcccgat atgctgaaca
    33901 ggttgcgcgt caaccgcctc acataaggca gtgcagattt cgatacgttc ggcacggtcg
    33961 tcgccgaata cgcgcacttt aatcagctca tgcgccgtca atgccgcatc ggtttccttg
    34021 atgaccgcgt ccgtcagacc ctgctgaccg accatcacaa caggatggag atggtgcgcg
    34081 cgcgctttca gttccaaaat ttctttggtg ttcaatttgg tatccgtcat tttcctactg
    34141 cttgaataaa aagaataatg cggcattgta cgcgatttgg cacgacatcg gcaaaattaa
    34201 cgtacaatac gcggtttcca ttccgacccg cgaaccacta tggctgtacg ttccaaatcc
    34261 tcaaaagcgt ggctgcacga acacgtcaac gaccactacg tccatatggc gcaaaaagac
    34321 ggctaccgcg cccgtgccgc atacaaactt ttggaaatca acgaaaaaga caaattaatc
    34381 aaacccggca cggtacttgc cgacttgggc agcgcaccgg gaagctggtc gcaggttgcc
    34441 gccaagctga cgggtacttc cggcgcggta ttcgctttgg acatcctacc catggaagcc
    34501 atagggggcg tctccttcat tcagggcgac ttccgcgaga acgacgtact ggcacaattt
    34561 gaaaccttgt tggacaaccg cccgctcgac cttgtaattt gcgatatggc gcccaatatg
    34621 tcgggaaacg ccgtaagcga tcaggcacgc agcttttatc tgtgcgaact ggctttagac
    34681 ttcgcctcgc aacacctgaa aaccggcggc agctttttgg tcaaagtctt tcagggtgca
    34741 ggctatcagg aatacatggc agccatgcgc gaaattttcg gcacggtgca gacgcgcaaa
    34801 cccgaagcct cgcgcaatcg ctccagtgag atttatttat tgggcaaaaa taaacgctga
    34861 caatacagac ggcgtgcttt acaatcattt ccgttttaca cctcaattat ggagccttgc
    34921 taagtgggga acacctttaa atcaatcctt gcctgggtcg ccttgggtat cggcctgatg
    34981 gctgcattca acgctttaga cggtaaaaaa gaagacaacg ggcaaatcga atattctcag
    35041 ttcatccaac aggtcaacaa cggcgaagta tccggcgtca acatcgaagg atccgtcgtc
    35101 agcggctacc tgattaaggg cgagcgcacc gacaaaagca ccttctttac caacgcgcct
    35161 ttggacgaca acctgattaa aacactgctc gacaaaaacg tccgcgtaaa agtaacgccg
    35221 gaagaaaaac cgagcgcgct ggctgccctg ttttacagcc tgctgcccgt cctgctgctg
    35281 attggcgcgt ggttctactt tatgcgtatg cagacgggcg gcggcggaaa aggcggcgca
    35341 ttctcattcg gcaaaagccg cgcccgccta ctggacaaag atgccaacaa agtgaccttt
    35401 gccgatgtcg ccggctgcga cgaagccaaa gaagaagtgc aggaaatcgt cgattacctc
    35461 aaagcgccga accgctatca aagcctgggc gggcgcgtgc cgcgcggcat cctgctggcg
    35521 ggcagcccgg gtacgggtaa gacgcttttg gcgaaagcga ttgcaggcga agccggcgtg
    35581 ccgttcttca gcatttcagg ttccgacttt gtcgaaatgt tcgtcggtgt cggtgcgagc
    35641 cgcgtccgcg atatgttcga gcaggcgaag aaaaacgccc cctgcatcat ctttatcgac
    35701 gagattgacg cagtcggccg ccaacgcggc gcaggtttgg gcggcggcaa tgatgagcgc
    35761 gagcaaacat taaaccaatt gttggttgaa atggacggtt ttgagagcaa tcagactgta
    35821 attgtgattg cggcaaccaa ccgtcccgac gtactcgatc ctgcgctgca acgccccggc
    35881 cgtttcgacc gccaagtggt tgtccccctg ccggacatcc gagggcgcga acagattttg
    35941 aacgtccatt ctaaaaaagt gcctttggac gaatctgtgg atttattgtc cctcgcgcgc
    36001 ggcacgccgg gtttttccgg cgcggatttg gcgaacttgg tcaacgaagc cgccctgttt
    36061 gccggccgcc gcaataaagt caaagtcgat cagagcgatt ttgaagacgc caaagacaaa
    36121 atctatatgg gtccggaacg ccgcagtatg gtgatgcacg aagacgaaaa acgtgcgacg
    36181 gcgtatcacg aatccggaca cgcgattgtt gccgaaagcc tgccctttac cgaccccgtc
    36241 cacaaagtaa ccattatgcc gcgcggacgt gcgctgggtc tgacttggca gcttccggag
    36301 cgcgaccgca tcagtatgta taaagatcag atgttgagcc agctctccat cctgttcggc
    36361 ggacggattg ccgaagacat cttcgtcgga cgcatctcca ccggcgcatc aaacgacttt
    36421 gaacgcgcaa cccaaatggc gcgcgaaatg gtaacgcgct acggcatgag cgacaaaatg
    36481 ggcgtgatgg tttatgcgga aaacgaaggc gaagtcttct tgggacgcag cgtaacccgt
    36541 tcgcaaaaca tttccgagaa aacccagcag gacatcgacg cggaaatccg ccggattttg
    36601 gacgagcaat atcaggttgc ctacaaaatc ctcgatgaaa accgcgacaa gatggaaacg
    36661 atgtgcaaag ccctgatgga atgggaaacc atcgaccgcg atcaggtact ggaaatcatg
    36721 gcgggcaaac aacccagccc gcccaaagat tacagccaca acctgcgcga gaatgcggac
    36781 gcggcggaag ataacgcgcc gcacgctccg actcgggaag aaaccgaagc acctgccccg
    36841 gcagacaccg cttcgtcaga gtccgagcag cggtctgaaa acaaggctta acttcccgaa
    36901 caaacggcag cccgcaagct gccgttttcc catcctgttt tcagacggca ttttccgctc
    36961 aatgccgtct gaaacgccaa agccgtatcg gtttttgcga atctgcgata tggcggttta
    37021 aattgaaatc agacgaggca aggctgtacc ggtttaaaat taaaccgcta taatactgcc
    37081 cttcgaatac acgcccgcat tggtgatgcc cgtgaaaaaa gttgaaaaaa acatcttggt
    37141 gctgcacggc gcggacaaaa tgttcgagct ggtcgacaag gttgaggatt acccgcactt
    37201 tctgccgtgg tacagcaaga ccgaagtcat cgggcgtagc ggcaacgaac tgaaggcgcg
    37261 gctgtttatg gattatatgc acgttcgcca atcgtttgcc acgcacaacc gcaacattcc
    37321 gggcagggaa atccgtatgg aactgctcga gggtccgttc aaaaccttac gtggaacgtg
    37381 gaaatttatc gatttgggcg acgatatgtg caaaatcgaa ttcaatttgg aatacgattt
    37441 ttccaatgcc gttttgtccg ccttaatttc ccccgtcttc aaccaccttt ccgccacgct
    37501 ggtcgaagcg ttcgtcaaag aggcagaccg ccgttatgct tgaaattgag attgtgtacg
    37561 ggctgcccga ccgacaggtt ttgaaaacca tgcagcttgc cgagggaaca accgtccgcg
    37621 ccgccgcact gcaaagcggt ttggacggca tatttgaaga tttaaacctg cattccgcgc
    37681 ctttgggcat tttcggcaaa gccgtcaaag acgacacgcc gctgcgcgac ggcgaccgca
    37741 tcgaagtgta ccgcccgctg ttgatcgacc ccaaagaagc gcgccgcaaa cgcgttcaaa
    37801 atcaagaaga ataaccatgc cgtctgaagc cttcagacag cattcgacag aaacacggaa
    37861 aatatcatgt caaacacaat caaaatggtt gtcggcttgg gcaacccggg caaagaatac
    37921 gaacagacac gccacaatgc gggtttttgg ttcctcgacg aactggcgtg gaaatggaag
    37981 gcttcattta aagaagaaaa aaaattcttc ggcgaagtcg cccgtgccgc cctgcccgac
    38041 ggcgacgttt ggctgctcaa acctgccacg ttcatgaacc gttccggaca ggcaattgcc
    38101 gcccttgcgc agttttataa aatcaaaccc gaagaaatcc tcgtcgtcca cgacgaactc
    38161 gacattccct gcggacggat taaattcaaa ctcggcggcg gcaacggcgg acacaacggc
    38221 ttgaaagaca ttcaggcaaa actcggcacg gcagactatt accgcctgcg cctcggtatc
    38281 gaccacccag gcgaccgcaa cctcgtcgtc ggctatgtcc tgaacaaacc cagtacggaa
    38341 caccgccgac agattgacga tgccgtcgcc aaatccctgc aagccatacc cgacatcctt
    38401 gccggcaaat gggaagaagc aacccgcttc ctgcacagca aatgacccga tgccgtctga
    38461 agccctttca gacggcatgt tcccgatttc catatccgaa cagtcatgac cgaactcaag
    38521 cagcttatcc aaaccgaatc catccccgtc atcgaagaaa ccctcgattt cctgctgtac
    38581 gaatgcagca tcgacgacgc accatccgcc gaagaagtgg cacaatggcg cgacatactt
    38641 gccgcacgcg gcggcaaatt cctgcgcctg tccaaaatct gccaaacgtg gctggacgag
    38701 gaggcggcat gaagctgccg cgcaaccgct tcagcctgct ttccgcattg tggtttgccg
    38761 gcggcatcta ttcgctgctc ttcaaagctg ccgacaccgc gccgccgccg tttccgcatt
    38821 tcgacaaagc agcacacctt gccctgtttt tcgcacaaat ctggcttttg accaaagcat
    38881 tcaaaaccgg aaaacttccc atcccctacc gcagcctgat ggtctttgcc ctctgtttcg
    38941 ccctcttcag cgaatgcgcg caggcatggt ttaccgcaac gagaaccggc agtttgggcg
    39001 atgttcttgc cgatatggca ggtacggttc tcgcactctt tgccgcccgc gccgccgacc
    39061 gcccggactg aatcggttta tttcccccaa acaggatgca cctcttcccg atacatccta
    39121 tttttcccct tacatatcaa tcccatctca taaacaaaac cgacaaatcg tttacaatat
    39181 atttacacta catcagatta caaatatact cgaaccagtt gcagaatcgg cggttccaca
    39241 aaccgtttcg gattgcacga cacaaaagaa aaagcgattt tattgcctta aaggagtatt
    39301 catgaacctg catgcaaagg acaaaaccca gcatcctgaa aacgtcgagc ttctcagtgc
    39361 gcagaagccg attaccgact ttaaaggact gctgaccacc attatttccg ccgtcgtctg
    39421 tttcggcatt taccacatcc tgccttacag tcccgatgcc aataaaggta tcgcgttgct
    39481 gattttcgtt gccgcacttt ggtttaccga agccgtccac attaccgtta ccgcgctgat
    39541 gatccctatc ctcgccgtcg tactcggttt ccccgacatg gacatcaaaa aggcgatggc
    39601 tgatttttcc aacccgatta tctacatttt tttcggcggc ttcgcgcttg ccaccgccct
    39661 gcatatgcag cggctggacc gtaaaatcgc cgtcgccctg ctgcggctgt cgcgcggcag
    39721 tatgaaggcc gccgtgctga tgttgttcct cgttaccgcc tttctgtcca tgtggatcag
    39781 caacaccgcc accgccgcga tgatgctgcc tctggcaatg ggtatgctga gccacctcga
    39841 ccgggaaaaa gaacgcaaaa cctacgtctt cgtcctgctc ggcatcgcct attgcgccag
    39901 catcggcggc ttgggcacgc tcgtcggctc gccgcccaac ctgattgccg ccaaagccct
    39961 gaatctggac ttcgtcggct ggatgaagct cggcctgccg atgatgctgt tgattctgcc
    40021 cttgatgctg ctctccctgt acgtcatcct caaacctaat ttgaacgaac gtgtggaaat
    40081 caaagccgag tctatccctt ggacgctgca ccgtgtcatc gcgttgttga ttttccttgc
    40141 cacagccgcc gcgtggatat tcagttccaa aatcaaaacc gccttcggca tttccaaccc
    40201 cgacaccgtt atcgccctga gtgccgccgt cgccgtcgtc gtcttcggcg tggcgcaatg
    40261 gaaggaagtc gcccgcaata ccgactgggg cgtgttgatg ctcttcggcg gcggcatcag
    40321 cctgagcacg ctgttgaaaa catccggcgc gtccgaagcc ttgggacagc aggttgccgc
    40381 caccttttcc ggcgcgcccg catttttggt gatactcatc gtcgccgcct tcattatttt
    40441 tctgaccgag ttcaccagca acaccgcctc cgccgcattg cttgtaccga ttttctccgg
    40501 catcgctatg cagatggggc tgcccgaaca agtcttggta ttcgtcatcg gcatcggcgc
    40561 atcttgtgcc ttcatgctgc cggttgccac accgcctaac gcgattgtgt tcggcacggg
    40621 cttaatcaag caacgcgaaa tgatgaatgt cggcatactg ctgaacatcc tctgcgtagt
    40681 attggttgct ctgtgggctt atgctgtact gatgtaaacc atcgacctaa acaacaagac
    40741 cgtctgaaag aatatttttc agacgacctt gaagtttgtc gttcagacac aatttgtcga
    40801 atcattcaaa accggattct aacgaaagga aacccatgat tatcctgcac accaacaaag
    40861 gcgacatcaa aatcgaactc gatttcgaca aagcccccgt tactgctaaa aacttcgagc
    40921 aatacgttaa agacggcttc tacgacggcg taatcttcca ccgcgttatc aaaggcttca
    40981 tgattcaagg cggcggcatg gatgaaaaca tgaacgaaaa agaaacccgc gatccgattc
    41041 aaaacgaagc gtccaacggc ctgcccaacg ataaatacac catcgccatg gcacgcactt
    41101 ccgaccccca ttccgccagc gcgcaattct tcatcaacac tgccgacaac gctttcctga
    41161 acttccgttc taaagagctg tacggcaaaa ccgtcgtcca agactggggc tatgcagtat
    41221 tcggtaaagt cgttgacggt tttgacgttg tcgatgccat cgaaggcgta tccaccaaac
    41281 gccacggcta ccatgacgac gtaccgagcg aacctgtcgt catcattaaa gccgaagcgg
    41341 tataaaccgg caatccgaaa gcagcctgca caaaggctgc tttttttgtt tcaggcggca
    41401 taaaatccaa gcacatatgc cgtctgaaca cctcccgcaa gccttcggac aatgttgcaa
    41461 cgtgttaaaa tagcctcctt tcctgaaacc acaacaagga aaccatcatg gcaaatattg
    41521 cccgcgacat cttcaaagcc tacgacatcc gaggcattgt cggcaaaacc ctgactgacg
    41581 atgccgccta tctcatcggc agggccatcg ccgccaaagc cgccgaaaaa ggcatgaccc
    41641 gcatcgcgct cggacgcgac ggacgcttga gcggtcccga actgatggag cacatccaac
    41701 gcggctttac cgacagcggt atcggcgtcc tcaacgtcgg tatggtcgcc acgcccatgc
    41761 tctattttgc cgctatcaac gaatgcggcg gcagcggcgt gatgattacc ggcagccaca
    41821 atccgcctga ttacaacggt ttcaaaatga tgctcggcgg cgacacgctt gccggcgaag
    41881 ccatccaaga gcttttgtcc atcattgaaa aagacggttt tgctgccgcc ggcaaacaag
    41941 gcagcgtaac cgaaaaagac atctccggcg aataccacaa ccacatcgtc ggacacgtca
    42001 aactcaaacg cccgatgaac atcgccattg acgcgggcaa cggcgtgggc ggcgcatttg
    42061 caggcaaact ctaccgcgct ttgaggtgcg gcgttaccga acttttctgc gacgtggacg
    42121 gcaatttccc caaccaccat cccgacccat ccaaacccaa aaacctgcaa gatttgattg
    42181 ccgcgctgaa aaacggcgat gccgaaatcg gcttggcgtt tgacggcgat gccgaccgct
    42241 tgggcgtggt taccaaagac ggcaacatta tttatcccga ccgccaactg atgctgttcg
    42301 cgcaagacgt gttgagccgc aaccccggcg cgaaagtcat cttcgacgtg aagtccaccc
    42361 gcctgcttgc cccgtggatt aaagaacacg gcggcgaagc cataatggaa aaaaccggcc
    42421 acagctttat caaatcctcc atgaaaaaaa ccggcgcact cgtcgccggc gaaatgagcg
    42481 gacacatctt cttcaaagaa cgctggttcg gattcgacga cggtctgtac gccggcgcac
    42541 gcctcttgga aatcctgtct gcctccgata atccgtccga agtgttaaac aacctgccgc
    42601 aaagcatttc cacgcccgaa ctcaacattg acctgccgga aggcagcaac ggccacaaag
    42661 tgattgaaga gctggctgcc aatgccaagt ttgaaggtgc gaccgaaatc atcaccatcg
    42721 acggtctgcg cgttgaattt cccgacggct tcggtctgat gcgtgcttcc aataccacgc
    42781 cgattctggt gttgcgtttt gaggcagata cgcaagaagc catcgagcgg attcaaaacc
    42841 aattcaaagc cgtcatcgaa agcaatccga atctaatctg gcctctgtaa acacaggaaa
    42901 aatgcttaat caaatcttgg tagattggat ttggcttaaa aatctccgcg tccgctcatg
    42961 tttgggatgg ttgaacaaat cttgcgggct gccttgttcg acaataacgc ctccgtccat
    43021 cacgacaacg gtggtcgcca cttctaaggc gaatttgatt tcatgcgtaa cgacaaccat
    43081 ggtccagcct tcttgcgcca attccttcat ggtatccaaa acatcttgca ccaattcagg
    43141 atcgagtgcg gaagtcggtt cgtcaaacaa catcagttca ggctgaatcg ccaatgcgcg
    43201 ggcgatgccg acgcgctgct gctgaccgcc ggaaagctgg tagggataaa ggttaacctt
    43261 atcgcccaag ccgacttttt ccagtaattt caaagcttct tcgcgcgctt gggcggcagg
    43321 cttaccctgc acggcaaccg gcccttccat cacgttttcc aacgcagttt tgtgcggaaa
    43381 gaggttgtat tgttggaaca ccatgccgga cttgcggcgc agtgccaaaa tatcgtgttt
    43441 gcttggtttt ttagaaaaat cgattttcag cggtcgctcg ttgtcgaact cgatttgtcc
    43501 gtcttcgggc atttccaacg cgtttaggca tcgcagaaac gtcgttttac ccgagccgga
    43561 aggcccgagg atgacgacca cctgcccttt gcacacatcc aaattgatgc cgcgcaaaat
    43621 ggtatttttg ccgaaggtct tatgaatatt gcggatttta atcatgacaa ctccttattt
    43681 ggcgacgtag cggtcgaaac gtttttccaa acgcgcctga atcaggaaca gcactttaca
    43741 aaaacaccag taaaccaaag cggcttcgat atagacgggc aaaaagtcat aagtgcggtt
    43801 tgccgtttcc tgcgcgacgc ggaacaattc cgttaccgtc acgactgccg cgagcgaggt
    43861 gtttttaaac aaaccgataa actcgttgct caaaggcggc acggcaacgc gaaatgcctg
    43921 cggcgcgacg atgcggcgga acgtctgcat ataggtcatg ccgatggaga aacctgcttc
    43981 ccattggcct ttcggtacgg acaaaattgc cgcgcgtatg gtttcggaag catatgcgcc
    44041 gacattgagc gaaaagccga tgatggcggc aggaatcggg tcgatataga tgccgacgga
    44101 aggcagcccg taaaacacaa tcacaagctg aaccaacagc ggcgtaccgc gaatgacgga
    44161 aatataaaat tccaccaatt tcagcaggat tttccgcacg atgccgccgg cgggcataat
    44221 ccgcaccaaa gccacggcta ccgcaatcat cataccgata acgaaagaag ctgccgccaa
    44281 aggcagagag accgcgaagc cggctttgac cataggcaaa aacgcgctga caatcatatc
    44341 ggcgcgtgtt tccgtcataa acggcagcga agcaaggaaa ttattgaaca ctgatgtctt
    44401 ttccgaagaa ttgttcgccc agttttttca gcgtaccgtc ggctttcagc tcgttgattg
    44461 ccgtactgaa tttcgccacg gcttcgtcat tgcccttgtt gacaatcagg ccggaaccga
    44521 ctttttcatc ggcaggtgcg gaccaaacga ttttcacgcc cgcattcggg tttttcttca
    44581 gatagtccaa aaccgccaat tcgtcgttca gggttgcatc ggcacgtttt tgttcaatca
    44641 gggtcagcga ttgcgccaaa ccgtcaacag ccaccaaatc tgcgcctgca gctttggctt
    44701 tttcgccgta gttgctggtc agggattgtg cggttttcac gcctttgatg tcgtcgatag
    44761 atttgatgtt gctgtcgtta cgggcaacca atacggcacc gctccagctg taaggatcgg
    44821 atttgtcgaa tgtcgcttgg cgttcggggc tggtcagacc gacttggttt gccaccacgt
    44881 cgaaacgccc cgccttcaaa cccgccatca tcgaatccca ttgcgtttct ttaaattcga
    44941 cttttacgcc taatttttcc gcaacggcgc gggtaacttc cacatcgtag ccggtcagct
    45001 tgccgtcttt gtcgtggtag gtaaacggtg cgtaagtgcc ttccgtgccg acggtaaccg
    45061 tacctttatt gttgatgcgc tcgattaaag aatcaccgga cgcgccggca gattgggccg
    45121 gagcagaaga agacgcgctg ccgccctctc cgccgcaggc tgccaaaacc aatgcggcaa
    45181 tgccgccgag tacgaatttt ttcaacatca ttctctcctg taaaaagtgt gcaagtgcgg
    45241 cattctaagt gaaattgcgg caaacacaaa gaatactttt tttatatggt tattccaaat
    45301 cggaatggaa aagatatgcc gaagccgccg gaaacggaaa taaagggggc gggaaaggcg
    45361 gcgggcggca cgaaaccgaa aagcggcatc aggtatgcaa acggcggatt cacgcacgcc
    45421 gttccggtaa aaatccgcct gccgattcgg tgctataatc catccgaaac agatgacacc
    45481 aaaaaggaac atctcatgaa cacgccccaa tccgccatca ttcccgacca cgcccaagcc
    45541 ggcatcttta tcgaagccga cttcgccgcc aaccgcctca acgatattaa agccgcctgc
    45601 cgcgcttcgc tcgacgcgtt aactgccttg aaagcccgct ttccggacga tattttgggt
    45661 ctgaccatcg ccttcggcag caaagcctgg gcaacattcg gacatacgga cgaaggcagt
    45721 gaaatcaaac ccttctccga aatgggcaac gggcttgcgc cgtccacgca gcacgatatg
    45781 tccatccaca tccaatcctt ccgccaaaac gccgcctacg cgcttgccca atccgttttg
    45841 ggcgcgttcg gcgacagcat atgcgtcgcg tccgaagaac acggtttgcg cctgtatcag
    45901 gatcgcggac tggacggttt cgtcgacggc acggaaaacc cgcagggcga tgaaaatgtc
    45961 cgcgaagtcg ccatcatccc cgaaggaaaa cccgatgcgg gcggcagcta tgtcctgctg
    46021 caaaaatacc tgcacgattt gaaaaaatgg gatgccgtcc ccgtcgccga acaggaggcc
    46081 tcggtcggac gcggcaagga aaccgacgac gaattcggcc gcgatgtccg cctgcccgat
    46141 tcgcacctcg gccgcgtcaa cctgaaagaa aacggagtcg gcctgaaaat cgtccgccgc
    46201 agcctgccct tcggcaaaat cagcggcgaa cacggtttga tgtttaccgc atactgccgc
    46261 acgctgcaca atatcgaagc gcaattattg agtatgttcg gcgatacgga cggcaaaacc
    46321 gacctgctgc tccgacatct ctccgccgcc gtttcgggcg gatactacta cgcgccgtct
    46381 gtcgaacgcc tgcaaaacct ctaagccgga cgatgccgtc tgaaaccatt tttcagacgg
    46441 cattttcccg ttttaccgtc caaccgtttt ccgagcgcgc actatgtctg cacccatcca
    46501 agcatttgac ccgctgaccg tccccatttc cggcaccaac ctgattgagg cctctgccgg
    46561 caccggcaaa acctacggca ttgccgccct gtttacgcgc ctgatcgtat tagaacaaaa
    46621 aaacgtcgaa cgcgtattgg tcgtaacctt caccaaagcc gccaccgccg agttgaaaac
    46681 acgcctgcgc gcacgtttgg acgatgtgtt gcaggtttta gaaagtaaag aaattgccaa
    46741 acttggagac gacacgcttt cagacggcat cgccgcctat tgtgcggaac accacaaagg
    46801 cgacaccttc ctgcccgcac tcttaaaaca ggctttgcaa aaagagggcc ggacgcgtct
    46861 gattgtccgc ctcaaggccg ccatcgggca attcgacaac gccgccatct ataccatcca
    46921 cggcttctgc caacgtatcc tgcgcgacta cgccttcttg tgccaagcac cgttcgatgt
    46981 cgaactgacc gaagaagacg gcgaccgcct gcttatcccg gcgcaagatt tttggcggga
    47041 acgcgtcagc aatgatccgg tgcttgccgc attggcgttt aaacgcaaag ctgtgccgca
    47101 aaccgtcctt gcccaaatcc gcgcctacct gtcccgcccg tacctgaatt tccgccgtcc
    47161 gcaggcggat ttgaaacagg cgcagcgcaa cgccgaaacc tcatggcaaa ccgtctgccg
    47221 cctgctgccc gagctggaag ccggcttttg gcgcatccat cccgacctca acggcaacag
    47281 ttaccgcaaa aacagcttcg gcaacctttt taaagaactg gcacaaaaat ccgccgccgg
    47341 acaactgccc tttctggaca aagacacgca cgacagactc ttgaaacttg cgtccgacaa
    47401 actcgaagcc ggactgaaaa aaggcaaaac gcccgatgcg gcagtatttg ccgaattgca
    47461 gaaactggca gacttcgggc gcgatttgaa cgcactcgaa gaagcggaag aagcaacaat
    47521 gatccggctg caactggatt taatcgaata cctcaaccgc agccttgccg agatgaaaaa
    47581 atcgcgccgc gaacgcggtt tcgacgacct gctgctcgat gtccacaccg cgctgaccga
    47641 caatccgcac gccgaaaccc tcgcccgcgc cgttgccgaa aactgggaaa ccgcgctgat
    47701 cgacgagttc caagacaccg acccgctgca atacgaaatc ttccaaaaaa tcttcatcgc
    47761 ccaaaaccga ccgctgttcc tcgtcggcga tcccaaacag gcgatttaca gctttcgcgg
    47821 agcggacatt tacgcctacc ttcaggcggc ggaagacgcg cggcaccgct acacgctcgc
    47881 caccaactac cgcagccacg ccgcgcttat cggcagcata ggcgcgctgt tccgcctcaa
    47941 agaacgcccg ttcgttttgg aaaacatcgg ctattcggaa gtcggtgcgg cgcgtgccga
    48001 aagcagattg tcgcccaaac gtcctgccgt acaggttcgt tggctgcacg aaaacgacaa
    48061 tgaaaaagcc aacaaagacg ttttgcgccg acgtgccgcc gactattgcg ccgacgaaat
    48121 cgcccacgcg ctcaacgaag ccgccggagg ccgtctgaat ttcaaaggct acccgttgca
    48181 gtcgggcgat attgccgtgc tggtccgcac gcacaacgag gcggtgatgg tttccgccgc
    48241 cctgaaaaaa cggcaggtgc aaagcgtcct gctttcgcgc gaatccgtgt tcgcctcgcc
    48301 cgaagccgcc gccctttccg cactcatcgg cttctggctc gaaccgcgcc gcgccggaac
    48361 gctgcgcttt gtcctgacaa gcggcatatt cggttatgac gcgcagcaat tgcacgactt
    48421 caaccaaaac gaaagcgaga ttttgcattg ggcggaatct gcccgaaccg cgctcgacaa
    48481 ctggaatcaa tacggcattt tcgccgccat gcagcaattt tcccaaacac acggcatcga
    48541 aacgcgcctc ttaagccgga acaacgggcg cagcctgacc aattatttcc aactgctcga
    48601 actgcttgcc gccgaagacg cccaaaaccg caaccccgcc gcgctgcaca aatggctgcg
    48661 cgaccaaatc agcctagccg acaacaacgg cggcgacaac cgcgccatcc gtctggaaag
    48721 cgacgaagat ttggtcaaaa tcgttaccat gcacgcctcg aaaggtttgc agtatccgct
    48781 ggtgtactgc ccgtttgcgt gggacgcgca agataccggg ccgtccgact ggcaaatcct
    48841 ccaccaaagc gccaaccgaa ccgaactgtt ggcaaaggcg caactgtcgg aagacgaaca
    48901 gaagcaatac gccgatgagg aaatggcgga acgcctgcgc ctgctttatg tcgcgctgac
    48961 gcgtgcagag gaacagctca acatctacgc cgcatactcc accaatactg ccgacacccc
    49021 cctcgcctac ctgattgaag gctcgcccca agacagccgc gaaaccgtcc gccaaaccta
    49081 tgcccaagaa aaggacggca tcgcgatgct caaacgcaac tggcggcgtt tggcggacaa
    49141 cgccccagcc ggcacggatt tcgtcttcac agaagatgcg ccgccccctg ccgcctatcg
    49201 cggcaacgcc gatcaaaccg ccgaatttgc cgcaaacagc attcccgaac gcggattccg
    49261 atttgtccgc cacaccagct ttaccacctt aagccgccat acccaaacgc ccgacggcag
    49321 tgaagaagat gcctgcccgt ccttggatgc cgccgaaacc tcagtgccgg caatgccgtc
    49381 tgaaacgccg acggcttcag acggcatatc gatacacaac tttccgaaag gcacgcaggc
    49441 ggggctgtgc ctgcacgaaa ttcttgaaaa tttcaaattc ggacaagcag ccgccggaca
    49501 ggaaaccttc atcaccgaca agctgaaaaa atacggattt gaagaaatat ggctgcctgc
    49561 cgttgccgaa atggcggaag cctgccaaaa aacgccgctg acgggggcat acggcctgtc
    49621 cgacatccta tccgagtgcc gctgtcccga aatgggcttt accctccaca ccgaagactt
    49681 cggtctcaaa cggctgcgcg actggtttgc ccgcgacgac atcaggctac ccgaagtctg
    49741 ccgtgccgcc gccgaaacgc tcgacttcca caccgtcaac ggctttttaa acggctttat
    49801 cgacatggtc tgccaagacc ccgacggcaa tatctgcgtc atcgactaca agtcgaacca
    49861 cctcggcacg gacgcatccg cctacacgca acaggcaatg gacgaagccg tcgcgcacca
    49921 ccactattac cttcaggcac tgatttacgc cgttgccgcc gcacgctact tcaagctgcg
    49981 cggacaaccg cccgccgccg tttccgtccg ctacctgttt ttgcgcggat tggatggcaa
    50041 aggcggcggc gtttggcgtt gggacataga tgccgacact ttggaacaga taaaataact
    50101 tcccgtccct taagacgaca accgcaggag aaccctatga atatcaaaca cctgattacc
    50161 gccgcactca ttgcctcagc cgcctttgcc gcgcaggcag ccccgcaaaa acccgtatcc
    50221 gccgcccaaa ccgcgcaaca ttcagccgtt tggatcgatg tccgcagcga acaggaattt
    50281 agcgaagggc atttgcacaa cgcggtcaac atccccgtcg accaaatcgt ccgccgcata
    50341 cacgaagccg cgcccgacaa agacacgccg gtcaacctct actgccgcag cggacggcgt
    50401 gccgaagccg cccttcaaga gctgaaaaaa gcaggttata caaatgttgc caatcacggc
    50461 ggttatgaag acctgctcaa aaaagggatg aaatgacgca atgccgtctg aaaggacagg
    50521 ctttcagacg gcatacggca ctccgacacg cggcgcaaca tcgggcgcgg cgcgtgccgc
    50581 cccgtccgaa gcctgataaa caaaccgata aggaacaatg atgaaacaca tactccccct
    50641 gattgccgca tccgcactct gcatttcaac cgcttcggca catcctgccg gcgaaccgtc
    50701 caccccaaac gaaaccgcta tgaccacgca taccctcacc tcaaaataca gctttgacga
    50761 aaccgtcagc cgccttgaaa ccgccataaa aagcaaaggg atggacattt ttgccgtcat
    50821 cgaccatcag gaagccgccc gccgaaacgg cttaacgatg cagccggcaa aagtcatcat
    50881 cttcggcacg cccaaagccg gcacgccgct gatggtcaaa gaccccgcct tcgccctgca
    50941 acttccactg cgcgtcctcg ttaccgaaac agacggcaaa gtacgcgccg cctataccga
    51001 tacgcgcgcc ctcatcgccg gcagccgcat cggtttcgac gaagtggcaa acactttggc
    51061 aaacgccgaa aaactgatac aaaaaaccgt aggcgaataa gcccgtcgcc gacatatgtc
    51121 ggatggaaat caaatgccgt ctgaaacccg atttcagacg gcatttccgt ttattccctg
    51181 ctgtcgcggc aaatatcgat ggcttcctgc aaactcgaaa cgccgtagat tttcaggttc
    51241 ggaaactctt tggcgttgcg cggcatattg gctttgggaa caatggcgcg tttgaagccg
    51301 agtttttccg cttctttgag ccgctcttgt ccgcgtgcga cagggcggac ttcgccgctt
    51361 aagccgattt cgccgaaaac cacggttttt tcgggcatgg ggcggttgcg gaagctggaa
    51421 agcatcgcga ggatgactgc caaatccgcc gccggttcgc cgattttcac gccgccaacg
    51481 gcattgagaa acacgtcttg gtcgaaacag gcgatgccgc cgtgtcggtt taacacggca
    51541 agcagcatcg caaggcggtt ttgttccagt ccgacggtaa ggcgtttggg ggtgaatccg
    51601 tgcgcgtcat cgaccaatgc ctgaatttca accaaaagcg gtcggctgcc ttcctgcgta
    51661 accaaaacgc acgaaccggg cgtgtcgtcg cggtagctgg caaggaagat ggcggacggg
    51721 ttggacacgc ctttcaaacc gttttcggtc atggcgaata cgcccagttc gtttgccgcg
    51781 ccgaagcggt ttttgatggc gcgtatcatg cggtagttgg aatgctggtc gccctcgaaa
    51841 tacagcacgg tatcgaccat atgctccagc acgcgcggac cggcaatcgc accgtctttg
    51901 gtcacgtgtc cgaccagtat catggcgatg cccatctgtt tcgccatgcg cgtcagttgg
    51961 gcggcacact cgcgcacctg cgacacggaa ccaggggcgg aagtgatttg gtcggaatac
    52021 atggtttgga tggagtcgat gacgacgact tcgggctgat gctgtttcaa ggccgtctga
    52081 atggcttcca tgcggatttc ggcaagcagg ttcacgcctt cggtcggcag ttccaaacgt
    52141 tgcgcgcgca gggcgacctg ttgggcggat tcctcgccgg aaacgtacag tactttgcgg
    52201 ctttgcgcca ttttggcgat ggtttgcagc agcagcgtgg atttgccgat gccggggtcg
    52261 ccgccgagca ggatgaccgc accgtcgacc aaaccgccgc ccaatacgcg gtcgagttcg
    52321 cccatgccgg tcggattgcg cggcacttcg gtggcggtaa cggcggagag ggattggacg
    52381 gtcgaagtgt ccgccgccca agattggaaa cgggcatttt tcggctcgag cgcggctaag
    52441 ctttcctgaa gtgtgttcca ctcgccgcaa tgcgggcatt tgccctgcca tttcggggaa
    52501 gtgccgccgc attcggtgca ttggtaaagg gttttaagcg tttttgccat gatgttttcc
    52561 ctatgggatt tttgaagtgt gaaaaaatcg tctgaaactt cttttctaaa aaatttttta
    52621 ttattagttt gttaattttg attacaagac catatcacga tacaaacaag ggtggctttc
    52681 tgttattttg tggcataatg acggaaaata caaaacagga atcccaaaca atgactttcc
    52741 cattaaatat aatggtagaa ttgcgttgcg ttgcgttgcg ttgcgttgcg ttgcgttgcg
    52801 ttgcgttgcg ttgcgttgcg ttgcgttgcg ttgcgttcag agcatagttc acctttaaaa
    52861 tccatacaag gatttttatc aaatttatgc ataggagtga aatcctatgc tatctaaaca
    52921 aatctcaaat cttaattctt ctagcaataa accaaaaatc ctatctctat tttcaggatg
    52981 tggcggtttg gatttgggct ttcatcaagc tggttatgaa actgtttggg cgaacgattt
    53041 ctcccattgg gcttgcgaaa gtttccgtaa aaatatcggc gatgtcatcg tagaaggtga
    53101 tattgaacaa attgatccga ataatccaac tattcccgat tgcgacatca ttttaggcgg
    53161 attcccttgt caagattttt ccatgatttg gaaacagccg ggcttagagg gtgagcgcgg
    53221 caatctttat aaaagctttt tacgttttgt aaatgcaaaa aaaccgaaag tttttgttgc
    53281 tgagaatgtg aaaggtttat tgactgctaa caagaaaaaa gccatccagc aaattattac
    53341 cgactttgaa aattgcggtt attacgttca ggcgaagctg tataactttg ctgaatttgg
    53401 cgtacctcaa tttcgtgaac gtgtgctaat tgtcggtgtg cgtttggata cgggtttcga
    53461 cttccaccac cctgagccta ctcacggtga aaatacggat tacggtcttt taccttatgt
    53521 aaccgctggt caggctattt ctgatattcc tgaaaacgca aacaatcacg agcagttaaa
    53581 aatcagtgaa caaactaaaa aacggttgaa tctgattcca gaaggaggaa attacagcga
    53641 cattcctaga gatcaccctt tatacgttaa aagtatgttg agtttggttt accgacgaat
    53701 gcatcgggac aaaccttcta caaccatcat tgctgctggc ggtggaggta cttggggtta
    53761 tcacttccct gaaccgcgtg cctttaccaa ccgcgaacgg gcaagattac agagttttcc
    53821 cgataatttt gagtttatgg gctcaaccac ggaagtccgc cgtcaaatcg gaaacgccgt
    53881 tcctccacag ggtgtagtag aactagcaaa agccatactt ccagtttttt cagacggtta
    53941 tcacaaggta aatctacatg aaaaattaga agcagaaaaa aacgtcttct tccaagacag
    54001 actgaacaaa atccgtgggg gaaaacgatg aatacggttt tttccaacat tgccaatgcc
    54061 aaaatcacag ataaatcctt gaataaggtt tggatggatt tgtttatgtc tgccgatgat
    54121 atgttgatgg cgacaggtta tgtatccaat gatgcagtaa aagaactgca taaaattttg
    54181 gaagaaagcg atcataaaca aaaactcagt ttgatggtcg gaatgcacta tttagaaggt
    54241 ttcagtcgcc cacaatacag cagcttatgc gaattgaacg actttttgca agatgaacag
    54301 cgtggaatgg tttatgtgtc gccgtgggta aagtttcacg ggaaaatgta ttcatttcaa
    54361 aatcagcggg aaatccatac actaatcggt tcggcaaatc tgacttgttt ttgggataaa
    54421 gccgaacgaa cttatgaaac gatggtgcat ttggatgatt atcaaatagc gtctaaattg
    54481 cgggaagata ttgctcaaac tattaagcag cttggaattc ctatcaacaa agcagataag
    54541 ccagtagagt ttaaagaaca caatgttcat ttagagcaat gtttaggtgt tgagaaaata
    54601 agtaaagaaa gagtagaaaa actatttact caaacttctc aatattattt tgaattacct
    54661 gttaaaactg aacctagaag cagtttaaat cgtttttttg cggaaggtag aaaaaatcaa
    54721 cggggctttt atttacctcg accttggtat gaggcagagt tgattgtttc aacaagattt
    54781 ttaactgcag agggttatcc aaaggaaaaa aactttacag ttattacaga tgatggctgg
    54841 cagtttgaat gtaatacgac aggacaaaat ggtaaaaatt tcagttcaaa aggtgaccaa
    54901 actatttttg gaaaatgggt aaaaggacgt ttggaagcaa ccggctgttt aaaaacaggt
    54961 gaacctgtaa cagaagaaac cttacgccaa tacggtaacg atcattttga gttccgttca
    55021 accgacaatc ctgatgtttg gctgctctca tttaagggga aaaattaaat gttgcgaacc
    55081 tacttgaatc aattaacccc tcccgaatta gctgactctg ttgaaaatac tgttaatggt
    55141 tttatggaga aattatcaca aaccgagccg aaaatcgccc aaaatgtttt attactgggc
    55201 aatgttcaaa gtggcaaaac ggcacaggtt ttgggtatat taagtgcgct ggctgatgat
    55261 ggtgaccaca aagttttttt gtatcttact actgacagcg tagatttaca ggatcaaaca
    55321 gtcaaacgag ctaaagccaa tctgaaaaac tttatcgtat tgtctgaagc tgacgaccga
    55381 agttttatgg aagtaatgaa ggcagaaaat cccatcttgg tcgtgattaa aaagaatgcc
    55441 cgtgtattga aacgttggcg taatttgttt gccagtcaaa gtagcctaaa aggctatcct
    55501 ttagttattg tggatgatga agccgatgct gccagtctaa atactaatac tgataagccg
    55561 gataaagatg ccagcactat caataaactg ctaaatgaca ttaagaattc ctgttgtcaa
    55621 agtctattta ttcagttgac cgccacacct caatcgcttt tgttgcaaaa cgaagcatct
    55681 gattggcaac ctgaatttat ccacttcttt gaagcaggcg aaaaatacat cggcggcaac
    55741 tttgtctttt ctgatccacc aagctatata gttcgtttta ttgatagcga attggatgat
    55801 atgaaagacg agagtggtga aattgccgaa ggagcaaaac aggcattgct tagttttcta
    55861 ataacctgtg cagaattcgc gctatgtgat aaggctaact gtaattttgc actacatcca
    55921 agctataaaa ttcaagatca tcaagccttt tcaaagaaga tccaagcctt tttaaatgat
    55981 ttagtgcaag cagtcaataa tggggaagac cttgcaggta gctttaaaga aagttatttg
    56041 gatttgcaaa agaccaagcc cgatattcat cactttgatg aaatatacga aaaattgaca
    56101 gcacttttgg agaataaaca aatctctact ctcgtcgtta actcgcagac agaatcagat
    56161 tttgatttgg aaagaggttt taatattatt attggtggaa atgtgatcgg acgaggttta
    56221 actattccga aactgcaaac ggtttattat agtcgcactg ccaaaaaacc gaatgcagat
    56281 actttctggc aacattcgcg tatttttgga tatgaccgcg ataaatcatt attacggctt
    56341 tatattccat ttgatgtcta ttacttcttt gtacaactca atcaagcaaa taatctgatt
    56401 attgggcagg caaaaaattc aggcggcaat attcaggtta tttatccgaa aaatatcaat
    56461 ccaactcgaa aaaatgtatt gaaattcgat agtatcaatc aaattgtggg tggtgtgaat
    56521 tattttccac ttcacccaaa cgaagataat ttatcggaga ttaacaaaat tttgccttct
    56581 attttgaaag atgaaattca atctgattta tatcaaatag atatagaaga tttattcttg
    56641 gttctagata aattgggtcg ttatgttcca gatgactgga ataaggaaaa atttattgct
    56701 ggtgtagaag cattaaaagc acaacgtcca agttttaaaa cctatgtttt gatcaaaacc
    56761 ggacgtaaac tttctcgagc aaccggcaca atgctttcgg aagacgaccg taaattagga
    56821 gaaaaatatc ccaatgattt attccttacg ctttatcaag tagtaggaaa taaagacaaa
    56881 ggttggcagg gaaaagattt ttggttaccc aatatcaagc taccacacaa tggcttggtg
    56941 taccaaagtg caaaatgaag catatgccgt ctgaaaactt ccgattcttc agacggcata
    57001 tcgggcagtt tcctgccggc ttacccgccg tgatactgcc gcgacaactc gtgtacggtg
    57061 tcgactaaga ttttggcgtg ttcggggtcg gcgtgttggt tgatgccgtg tccgaggttg
    57121 aagacatggc cgctgccttg tccatagtcg accaggatac gtgcgacttc ggtgcggatg
    57181 gattccggcg taccgaagag ggcgaacggg tcgaagttgc cttgcagggc gacttggttg
    57241 ccgacgcggc ggcgtgcttc gcctatgttg cacgtccagt ccaagcccaa tgcatctgcg
    57301 ccgatttggg ccatactttc cagccacagc ccgccgcctt tggcaaatac gataacaggc
    57361 acgcggcggc cttcgctttc gcgtttgagt ccggcgacga tctggcggat gtatttgagg
    57421 ctgaattctt taaacgccgc atcgctcaac acgccgcccc aagtgtcgaa aatctgcacc
    57481 gcctgcgcgc ccgcgtcgat ttgggcgttg aggtaggcgg taacggcttg ggcgttggta
    57541 tcgaggattt tgtgcagcaa atcggggcgt gagtacatca tggttttgat ggtgcggaat
    57601 tctttgctgc cgccgccttc gaccatataa caggcgagcg tgaacgggct gccggagaag
    57661 ccgataagcg gtacgcggcc gtccagtgct ttacggatgg agctgacggc atcaaagacg
    57721 tattgcagtt tttccatatc gggaacgtgc agcttggcga tatcggcttc gtgttgcagg
    57781 gcgcgtttga atttcgggcc ttcgccttcg gcaaagtaca gtcccaagcc cattgcgtct
    57841 gggacggtca ggatgtcgga gaacaaaatc gccgcgtcca aatcgaagcg ttccaaaggt
    57901 tggatggtaa cttcggtcgc caatccggtg tttttgcaca aatcgaggaa gctgcccgct
    57961 ttcgcgcgtg tggctttgta ttcgggcaga taacgccccg cctggcgcat catccaaatc
    58021 ggcgtgtatt cgacgggctg tttaagcagg gcgcggagga aggtatcgtt ttttaaaaga
    58081 gtcatacggg ttttctgtca gtctgtttgt caagtgaaat ggttttcaga cggcatttca
    58141 acgatgccgt ctgaaggttt caatgggttt cggcggaagg tcggttttcc tcggcaacgc
    58201 ttgccaaaac caagttgcgc tgcgcctccg ccttctgcgg ttctccggtt tcgtcaaaaa
    58261 cctttgccag aaccaaacgc gcggaaatac tcggctttaa tgcaatgctc gcttcaaggt
    58321 agccttttgc cttgccccaa agtttgcggc cgtaggcgag ccgaccgaga tacatcagca
    58381 gaagcgcgtt atcgggctgt tctttcagcc aagcatcggc aaaatcgatg gctttctgct
    58441 gttcgcgctc gcccaaaaag cgcacgcttt cgacaaaggc ttccaaaagt tcggggcggc
    58501 ggttgtgcgg ataatgctgt ttgacccatt tgaccgcatc ggcatacagt cccaaacgtt
    58561 cgtacttttc cgcaaccgat acgctcaatt ccccgttttt gaggctgtcg ggaatccgct
    58621 tcaggcaggt tttcaaagcg gcggcatcgg cagcatccgc cagctggcgg cggtatgccc
    58681 aattttgata ccgttccatt tccgatttgc ccaacgcgcc cgccttggaa agtttttcgg
    58741 tttttgccag aacctgcaac gcgtcgcccc tgtcgaaagc gtaacgaagt tgcagacgca
    58801 cgaggcgcgt aaggttggca ttcatcttcg ccgccgcatg aagattggct tccgccgctt
    58861 cgtaatcgcg ccggttcaac gccgattccg ccaacaaaag ataacgggaa agctgctgtt
    58921 tttccggcag tttggcgatt tccgcaagat aacggtcgcg cagctcgatg ttttccatct
    58981 gtccggcggc gtgcgcaccc agcatcaatg ccaaagtccg gttgtcgccc gcctctttgt
    59041 tgcccaacac gcgcgaggct tctagttccg ccttttcaaa acgcccttca aaatacgcca
    59101 aacccgcttt gttcaagtaa agtgcggcct tgcggccttt ccgcgccgaa ccgaaacgct
    59161 gcatcttttc ggggatattg agtacgccga tgatgaattt gaacaggaaa taccacacca
    59221 cgacagcaat cagcgaacct aacacaaagg cgtgcaggtt gattctgagc atggtctgtc
    59281 cgagtacgat atacacgtcg ccggtgtaaa tgcccgaagc cagcgccagt ccgacggcgg
    59341 cggcaaacag gacgacaatc cagactaccg ttttcatgcg cgttctcctt tagcggacgg
    59401 cgaacgccct gccttttccg gtacgggctg tttttttgca ggctgttccg gtttgcgttc
    59461 cgaaggcagg gacggtgctt ctatggtctt cacatccgat gcggcagccg gttcggaagc
    59521 tgtgttttcg tttgccggtt cggaagccgc ctgttccgct tcttgatttt ccgccgccgt
    59581 catgcgcgta ccgtcgcgat aggcgcggac ggcatttagg ctgtttttca aaccgtcatc
    59641 cgccgtcatg cgcacatcca acgccttcaa ttccgccagt tctttcagcc acgactgcgt
    59701 ggcgggagac ttggcatcaa aatactgtct gacggcggtt tcggcattgt tcaaatcgcc
    59761 ctgatagact tcgccgttgc gctgcattaa tgcaatgcgc gcatccaaaa ggcggaggcg
    59821 caggttttca cgcacaaaat atgcctgttc cggtgaaagc agcatggcat cgttgttttc
    59881 caaacggcgg atttcgacca gccccttcaa tgtgccgagg gatttttccc atacgttctg
    59941 ccaccatgaa gcggaaacgg cttcgttgcg tgcctgtacg cccggtttca gcacgccgtc
    60001 gagcatcagc ggcagtccgg atacggcggt ttccagcctg tcgaggcgca atgccgtgcc
    60061 ggaaatatcg acatagggac ggtttttcag ttccgccaag tcgctgctga ccgcctgctt
    60121 gatcggcaga agctctgcct gattgaaacg ggacaggcgg ctgtcgatat gctccaatac
    60181 gccgactgcc gtttggatat tgccagtcaa caccagctgt tgcgccgcca gattgagtat
    60241 ggtctcggtt tcgtccacca gccaatcggc gcgtcctttg gtcaactcgc gataggattt
    60301 ttgcatttcc aagatttgtt cgccgtttgc tttgacgttt ccgtccaaac ggtcgagctc
    60361 tgattgtatg gcggcttgcc ggttgaggtt gtctttcaac agggcggcgt tttccgactc
    60421 gcccaaggcg gctttgtcga ttttttggtt gaatgccagc tcttggtttt tcaagacatt
    60481 ctgtccttgg acaaacaaaa aaccacttgt acccaaaccc aatgccgcca ataccagggc
    60541 gcagactgcc aaagcgttgc tgccggactg tttgacgata aaaggagcgg caggaatgtg
    60601 tacttctgtt tcgttttctt tgcgtgggga agaggtttca gacggcattt cttttgatgc
    60661 ctgtatctcg cgtacgggtt cggatgattt gttttcaggt tcgcccaccg ttttgctcct
    60721 tattaatttg aggttccggg aaagaccatg ccgtctgaaa cggaaatgga agaatgggaa
    60781 agcgcggctt ccagcgtagg gacggtttcg accgaacaca cgccttcgcg cttcaatgcc
    60841 tccgcaatgc gcggatgatg ggtaaagtat agcaaggatt tgaagaatcg ggaaaattgc
    60901 ggcggaagct gtccgaacag caagcgcacg agttctgtgg acgtaatata ggcggcggca
    60961 atattttcgg tttggaaatt ttgaaagttc aaaggtttat gccgtctgaa atagacttct
    61021 gccacctccg tccgaaaacc tttctcctgc aaggcattca tcagaaaatc ccgcccgccg
    61081 tgtccgcgca caaacaatac gcgcgcacct tcgggcagac tgttccaaac cgtcaggcgc
    61141 aaaaccgcct cgctgtcgtt gccgtcttcg ggcgcgatga ccgttctgcc caaacagcgt
    61201 tccaatgcac ggcggctgcc ctgccctacg gaaatgtgca tctttatgcc gtctgaaagg
    61261 ttaaggtacg ggacggcggt ttcaacggcg gtcggactga cccaaaacac ggcatccgca
    61321 cgggcatatt gttcggacaa aagttccagt ccggcagcat ctgcttcgat ttcaatcggg
    61381 ctcaacactt ccgcctgcca tccggcacga cggcaggttt ccacatcgtc cttcgccctg
    61441 cccgacggac ggacaatcag catagtcggc atcattaaat tccattatcg gggattacca
    61501 ggcaaaatcg tcatcgtcga aattcgcctg cgcggttttc gacgtacagt cgaaccgtgc
    61561 gatttccttg cccctacgct ccacaaggac accgctgcct actgcatggg tcacggaatc
    61621 gaagcctgca tctacggtgt acataaattc gccgttttgg aatttcatcg tcgcccaacg
    61681 gcctctgccc cagccttgcc acctgtcgga acgccccaac agatcagctt tgctgttgcg
    61741 tatggcaatt tctttttttg ccgcactgcc gaacgaatat tcgtaaagat tgcggttaat
    61801 tttttgaact tctatatatt tgttgttgtc cgttttacag gaaaacaccg tatctgccca
    61861 cacggtttgg gtcgcagctg acagaagcaa tacggcagaa aggaacgttt ttttcatcgt
    61921 atttccttaa tggttaaaac cccgccactt ggacatccgt ccttcgtggc ggcagcatca
    61981 gattttattt gagaggggtg taaccccttc caaattaggg caacacatag ggcaacgctt
    62041 tatgtgtcgt cctgtgtgtt gaaacatcat tgcccgtatc ctgaaataga aaagaaaatg
    62101 cccgttttac ggtcttcttt ccgacaaaat gccgtctgaa acggatttca gacggcatgg
    62161 tcgaaaatta ttgcgcggca agcggcttga gcagcaaggt ttcgccgctt tgcacaactt
    62221 tttcaagcag gtcgatattg ggatgcttgc cgggatgggc gcgcgcgtcg gcaacgtgtt
    62281 cggcaaacca tatcaaaccc tgttctgccg cttctttgtc caattttccg tcaaaacgtt
    62341 ccgccaaagc attgtacagc ttgagcgaac cgagcttgcc ctgaacggcg ggaatgtggt
    62401 gaaccgtttt gccgtcagca tcccgaacgt ccaaaccgcc cagatggtcg attgaaggaa
    62461 atgtggcgag atagtcttga aaattcatat caactccgaa aatcagtcag ttagcgcgtg
    62521 ccgaaaatct tgtcgcccgc atcgcccaga ccgggaataa tatagccgtt ttcgttcaaa
    62581 tggctgtcga gcgcggcggt gtaaatcgta acgtcagggt gcgcgtcatt aacagccctc
    62641 acgccctcag gggcggcaac caaaactaag gctttgatgt tgcggcagcc tttttctttc
    62701 aacaggtcaa tggttgccac catcgaaccg cctgtcgcca gcataggatc gataatcaaa
    62761 gccggacgtt cgtccatgct gtccacgaat ttctcgaagt aggaaacggg tttcagcgtt
    62821 tcctcgtcgc gctgcagtcc gactacgctg attttggcag tcggaatcag gtcgagcacg
    62881 ccgtcaagca tacccaaacc tgcacgcagg atgggaacga cggtcagggt tttgcctttg
    62941 atgcgatcgc cttcaatctg accgcaccat ccgtcgataa ggtatttttc gatttcaaaa
    63001 tcacggcttg cctcgtatgc catcaggcgc gccagctcga tggcaagcgt ccggaatttg
    63061 taggtgctgc aatccgcctc cctcatcagg gttaatttgt ggcggacgag cggatggttg
    63121 ataacattaa cgttcatcgc ggtgttccag tgtgattttg agacgcgcca ttatactttt
    63181 ttttgatttg ccggaaaaga aaatgccgtc tgaaacacgt tcagacggca ttgccgaatc
    63241 catcaggctt tcagcagctc ttgcagctcg cctgcctcat acatttccat caggatgtcc
    63301 gaaccgccga caaactcgcc gttcacataa agttggggga tggtcggcca gtcgctgtat
    63361 tccttaatgc cttggcgtac ttcgggattt tccaatacgt tgacggtaac gtaatcggtg
    63421 cagcctgccg cgttcaggat ttgcacggcg cgggaagaga aaccgcattg cggaaactgc
    63481 ttcgtacctt tcataaacaa tacgacgcgg tgtgtcgtta ctacttcttt aatttggtcg
    63541 tggatggaag ccatcgctta ttccttttga taaagttgac aaaatttgtc aggtaggacg
    63601 cattatacgc aaaagcggaa acttcccaca atatttgccg tatatcgcgc agtatcaatg
    63661 ttgtgaacgg aaatattccc actcctcgac aatcctactg tccggcaaat ggcacagggc
    63721 atatccgccg ccgctttcgt ctttttttac ttcccgtctg ccgcctttgg caatgcagaa
    63781 tttggaagcg gaatttgcca tgcctgtccg aaacgtgttg gcaggcagcc ggcagcagcg
    63841 cggatatgca tacggatgaa ttgcctccat ggttttcctt atcgtcggta cagggtcaga
    63901 cggtccgcat atcgggtact tttttaaaca gccggtaaaa gttttccgtc gtatatgccg
    63961 caacctgttc caatgtttga ttccgcaatt tggcgatatg ttcggcggta tggcgcacaa
    64021 aagccggttc gttctgcctg cctcgtttgg gaacgggggc gaggaacggc gcatcggttt
    64081 ccaccaaaat gcggtcgtcc ggaacgtatt ttgccgcctc ctgaaccaag ggtgcgtttt
    64141 taaaggtaac gattcccgag aaagaaatat aaagccccaa atccattgct gcacgggcaa
    64201 aaccgatgtc ttcggaaaaa cagtggataa cgcccgaatt aacccggcat tctttcagga
    64261 tagacaaggt gtccgccgcc gcatcacgcg tatggacgat aacgggcagt ccggtttgat
    64321 tggctgcttc gatgtggtct gcaaagcgtt tgtgttgcca ggacaaatcg cctttgcacc
    64381 agtaataatc caagcccgtc tcgccaatgc cgaccacttt cggatgggcg gctgcagcaa
    64441 ccatttccgc aatagaaaat tcttcggctt ccttgctgtc gggatgtacg cctattgtgc
    64501 agtaaatatg ttcataaact tcggcgatag cggatacttc ggcaaaactt tccctgctga
    64561 cgctgatggc gagcgcctgc cccacgccgt ttgcttccat gttggacaaa acttcgggca
    64621 ggcgttcttt caaaccttca aaattgaggt ggcagtgcga atcgatgata tgcatggcgt
    64681 tacatggtaa acgtaggtct gctgccgccg attgtgttgc ccagttcgac ttcgatctgg
    64741 tctttgactt tcaggcagtt ttcgtgtttt gtgaatgcca gccccacccc tttgggtttg
    64801 gaggaagtac gcgcaggatt gatccaggcg actttggtcg gcaggaacag tttggggaag
    64861 ttgaggattt ctacggcaag cagaatatcg tccccgatgg aaaatacgtc gttggtctgc
    64921 acaaacagac cgccgtgttc caaaaacggc atgtaggagc tgtacagcag attcatgtct
    64981 ttcagctgca acgacatcat ttttgccgga atattttgtc cgtctgacat aattcacccg
    65041 ttctctttct tcaattccaa atagttgatg agcagatgct cgatctgcat tttgacattt
    65101 aaagtatgaa acccgtaggg ggcaagctgt ttgagcatat cctccgccgc aaatacattg
    65161 cgcggacgga aaccggatac cgtctgaagc agcctgtctt cataagcggg ataatagacg
    65221 ggtttcatgt gttgcaggca caatcccaaa tcgaccagcc atttctgcat ccacccgaca
    65281 aatacggcga gcggaagttt ttccttatcg aaaagcgcgg cgtaatccaa aatcttcaac
    65341 aaccttggtt ctgccaaaat atccaacagt ttaatccgca acgcacggac accgtccgcc
    65401 tcatcaaaca gcggcgctcc ggaatggaaa gccagacgtt cctcaggttc cgccacaccc
    65461 ctttcacgca gatatgccaa tgcctcttca tgggaaggcg cgggcaaaac catcttccgg
    65521 cagcggcttt taatggtcgg taaaaccttg tccgccgcgt ggctgaccag caaaaagacc
    65581 acttgtggcg gaggctcttc caacactttc aacaaactgt tggcggcctg gatattcata
    65641 ctttccgcag gatgaatcag aaccacgcgc aaaccgcccc gtaccgaagt caggtacaca
    65701 ttatcgatga tttccctgac ggcatcgatt ttgatctgca acagtttgcg tccgttttcg
    65761 ggttcgtccg acaagggggt gatttcgtaa aaatcgggat ggcttccctg tccaaacaga
    65821 tggcaggaca tacattcgcc acagggtttg cagcccggtg caggggtttc gcacaacagt
    65881 gccttcgccg caaagcgggc aaatgtagtt ttccccgtat cttttttgcc ggcaaacagc
    65941 catgcattgg gacgacgctc ccaatgttcc gcaatctgcc gccattgctc attatgccac
    66001 ggataaatca tttcaaacca tccgtccgga aatccatacc gccatcagag cagttccaaa
    66061 tcggggctgg aactttgcgc gcggcggctg cgcaactgca ccgccaaacg caaatcgtgt
    66121 gcggaatcgg catttttcaa ggcatcctgc aaggaaatct cgcctttttc atacaattgg
    66181 taaaggtgtt ggtcgaaggt ctgcataccc aaggtagtgg attttttcat cacttctttg
    66241 atttcatgga tgttgccgtt gtgaatcaac tccgaaatca ggggcgaatt gagcagcacc
    66301 tcgactgccg ccaccctgcc cttgccgccg tctcgcggaa cgaggcgttg cgaaataaac
    66361 gcctgaaggt tgagcgacaa atccgtcagc aattgttcgc gccgctcctc ggggaaaaag
    66421 ttgatgatgc ggtcgagtgc ctgattggtg ctgttggcgt gcagcgtcgc catacacaaa
    66481 tgccccgttt cggcaaaggc aatggcgtag tccatcgttt cgcggtcgcg gatttcgccg
    66541 ataaggatga catccggcgc ctgacgcagc gtgtttttca acgccgccat ccagttttcc
    66601 gtatccacgc cgacctcgcg ctgggtgatg atgcagtttt tgtgttcgtg gacaaactcg
    66661 atcgggtctt cgatggtgat gatgtgtccg aacgaatttt cattgcggta gtcgataagc
    66721 gaggcaagcg aagtcgattt gcccgagccg gtgccgccga caaaaataac cagcccgcgt
    66781 tttttcagcg cgacatcctt caagaccggc ggcaggttca ggctttcaaa cttgggaatc
    66841 ttgctggtaa tcgcacggaa taccaacgcc gtcgcgccgc gctgtatcat cgcattgacg
    66901 cggaagcggc tggtgtccgg cagactgatg gcgaagttgc actcgttggt cgatgaaaat
    66961 tcttccgcct gcttcgcact cataatcgaa aaggcgattt ccatacattt ttccgccgtc
    67021 agcggctcgt ccgtgatgcg ggtgattttg ccgtccaact tcatcgcggg cgggaaatgg
    67081 gtcgtcacga acaggtcgga acctttgttt tggttcatat ggcgcagcca tgcgaacagt
    67141 tcttcctttg cggtcatatt ttcatcggta aacatcctcg tcccctaatc ttcaaacaat
    67201 ctgtaccttc ccattctacc aaaaacccgc cgtgccgtct gaaaccgttt cagacggcac
    67261 ggacaaacca cctccactca atcctgccgt gttagcgtgt ataatcaggc attctatccg
    67321 aaattcacag caggtcagca atatgtcttt gaaaacagta accgttatcg gcgcaatgga
    67381 acaagaaatc gagcttttgc gcgagatgat ggaaaatgtc aaagccgtct ctttcggcaa
    67441 gtttgccgcc tatgaaggcg aattagcggg aaaacgcatg gtgcttgcat tgagcggcat
    67501 cggcaaggtc aacgcggcgg ttgcaacagc ttggcttatc catcaattcg caccggactg
    67561 cgtcatcaac accggcagcg cgggcggttt gggcaagggt ttgaaagtcg gcgatgtcgt
    67621 ggtcggcaca gaaatcgcgc accacgatgt cgatgtaacc gcattcggct atgtttgggg
    67681 acaagtgccg caactgcccg ccgtatttgt ttcagacggc ctcttggtcg gcaaagcaaa
    67741 acaggcggcg gaagtgtttg aaggcgcggc ggtagaacaa gggctgattg tcagcggcga
    67801 ccgcttcgtc cacagcagcg aaggcgtggc ggaaatccgc aagcacttcc ccgaagttaa
    67861 ggcggtagaa atggaagcgg cggcaatcgc ccaaacctgt catcagttgg aaacgccttt
    67921 cgtcatcatc cgcgcggttt ccgattcggc agacgaaaag gcagacatca gctttgacga
    67981 gttcttgaaa acggcggcgg caaattccgc aaaaatggtg gcagaaatcg tcaaatcttt
    68041 ataagacctt tatcaaactt atgccaaaac agcggttttc cgttagaata tcgggctatt
    68101 tccacgttgc gcccgatatt tggacgtacc gctgtttgtt cagacggcat ttctaaaacc
    68161 taccgttcag cattaaaaac aactcaatga aaaatatccg aaatttctcc atcattgccc
    68221 acatcgacca cggcaaatcg acgcttgccg accgcttcat ccaatattgc ggcggtttgg
    68281 atttgcgcga aatgagtacg caggtactcg attccatgga catcgaaaaa gagcgcggca
    68341 ttaccattaa agcgcaaacc gccgcgctca actacaaagc acgcgatggg caggtgtatc
    68401 agcttaattt gattgacacg ccgggacacg tcgacttctc ttatgaagtt tcccgctcct
    68461 tgtccgcctg cgaaggcgcg cttttggtgg ttgacgcgtc gcaaggcgtg gaagcgcaaa
    68521 ccgtggcgaa ctgctatacc gcgattgatt tgggcgtgga agttgtgcct gttttgaaca
    68581 aaatcgactt gcctgccgcc gaccccgaac gcgtggaaca ggaaatcgaa gacatcatcg
    68641 gcatcgatgc cgtcggtgca gtacagtgtt ccgccaaaag cggtatcggc gtggaagatg
    68701 ttttggaaga aattgttgcc aaaatccctg cgccgaccgg cgacgaaaac gcgccgctgc
    68761 aagcggttat cgtcgattcg tggtttgaca actatgtcgg cgtggtcatg ctgattcgag
    68821 tgaaaaacgg caccatcaaa ctgaaagaca aagtgcgctt tatgagcacc aaggcggaaa
    68881 cgcaggtcga gcagctgggc gtattcacac caaaatcagt tcaaaaacaa gaactcaaag
    68941 ccggcgaagt gggctttttg attaccggcg taaaagaatt gggtcaggcg aaagtcggcg
    69001 atacggttac actggttgcc aaccctgcca ctgagccgct gcccggcttc caagaagtac
    69061 aaagccaagt attcgccgga ctttatcccg tggaaagcca cgactacgaa gccttgcgcg
    69121 acgctttgga aaaattgcag cttaacgacg cttcgttgaa attcgagcct gaagtttccc
    69181 aggcattggg cttcggcttc cgttgcggct tcttgggtct gttgcacttg gaaatcgtgc
    69241 aggaacgctt ggagcgcgag ttcgatatgg atttgattac caccgcgccg acggtggttt
    69301 atgaagtcgt gttgaaaaac ggtgagaaaa tcgaagttga aaacccgtcc aaactgcccg
    69361 acatcggcag catcgaaacc atactcgagc cgattattac cgcgaccatc cttgtgccgc
    69421 aggaatacgt cggcaacgtc atgactttgt gtaaccaaaa gcgcggcgtt caggtcaata
    69481 tgcagtacat gggccgccaa gtgatgctga cttacgattt gcccatgaac gaagtcgtca
    69541 tggacttctt tgacaaactc aaatccactt cgcgcggtta tgcttcgttg gactaccatt
    69601 tcaaagagtt ccaaccgtct gatttgatta agctggatat tatggtcaac ggcgaaaaag
    69661 tcgatgcgct gagcctgatt gtccaccgtc aaagcgcggt tcaccgaggc cgcgagctgg
    69721 catcgaaaat gcgcgagctg attccgcgcc aaatgttcga cattgccgtc caagccgcca
    69781 tcggcagcca gattatcgcc cgcgaaaacg tcaaagcact gcgtaaaaac gtcttggcga
    69841 aatgttacgg cggcgatatt acgcgtaaga aaaaactgct tgaaaaacaa aaggcaggta
    69901 aacgccgtat gaaacaagtg ggcaatgtgg aaattccgca aagcgcgttc ttggcgattt
    69961 tgcaggtgag cgacaaataa ctctggcaca cagggcggcg cgtcgggaac acccctcaaa
    70021 ccgccctgat ttcaggggca cgcgcccgca aacaaatttt gcaatttgcc gccttaaaac
    70081 ggttgcacgg acgggcggtt ttttaagcat taaggaataa caatgaacac tatgctaatg
    70141 tcgggcgcgg ctgccgcgct gcttgccggc atcatccttt atttcaaaag cgacaaaaag
    70201 cggcaggaaa acggtgaatg gagttccggc ctcgaatacg cctatatcct gacagcggtc
    70261 ggcgtgtttg ccgctttgtc cctgtttatg agctttaccg ccgttttcct gattttcgtt
    70321 gtattgtgcg gtacggcttg gggggtatat aaataccgcc tgaagactca tcccgaaatc
    70381 tcggaaagca gccacttcgg cgattatttc ggcagtttct tccctaccgt tttggtattg
    70441 ttcctcatcc ggtcgtttat cgccgaaccg ttccaaatcc cgtccagctc aatgcgcccg
    70501 ggcctgatca agggcgattt cattttggtc ggcaaatttt cctacggctt gcgcgtaccc
    70561 gttttaaaca atatatttat ccccacaggc aaaatcgaac ggggcgatgt cgttgttttt
    70621 aattatcctc tgcagccgga gatgacctac atcaagcgta ttgtcggcat tccgggcgat
    70681 gtggtcgaat atcgggataa gattttgacg gtaaatggca aacccacttc cgatattcct
    70741 aacggcacat accgttatcc cgacgatacc gacccttccg aaatccacaa cacggatatg
    70801 ttccgcagcg gtctagacgg caaatccttc aatattctga aaaaagaagg acagcctgcc
    70861 gtttccctgc ccgtattggg caaatatacc tccgatatta tgtctgaaaa cggatattcc
    70921 atagagcaaa gcggtttgga acactgccaa tatgccgacg acggcagcgg tttcgtgtgc
    70981 aaagttcccg aaggacgcta tttcgctatg ggcgacaacc gcgacaacag tgccgattct
    71041 cgctactggg gatttgtgga tgacaagctg gttgtcggca aggcaatgtt cattttgatg
    71101 aacttcggcg atttcggcag ggccggtacg gcaatccgtt aggccgggaa gccaagaatg
    71161 ccgtctgaaa atagtttcag acggcatttg ctattcctta cctttgcagg cgggtctaac
    71221 gttgtcggat tttcctacct atagtgaatt aaatttaaat caggacaagg cggcgagccg
    71281 cagacagtac aaatagtacg gcaaggcgag ccaacgctgt accggtttaa atttaattca
    71341 ctatatcagt ccggtaacgc aggttttgca cggcgaactg ccaatattgc cctgatgaaa
    71401 tcaatgtgaa gactgctgtt tcagcgaacc gagataaatt gccgaaagcg tcagcgccac
    71461 ccctgcccac tgcacgcctt caatcggttt gcccaatccg aaataatcga tgaacagggc
    71521 ggcaaccggt tcggacaaaa gcagcagccc cgtcagcgac agcgaaagca gcggaatcgc
    71581 ataggcaacc atcgcccacg cgaagcactg catcaccacg ccgtacacaa gcaccaaacc
    71641 cgcatctttc caagtcgtcg gataaagcgc gccgccatcc atcagcaatg ccggaacaac
    71701 caacgatacc gcgccgccta aactcaaaat catcattgaa gggaaaagcg ccaccggctc
    71761 gacttcatga gtcttgcgga cgaacaccat cgacagggca agcatcagcc cggacaccaa
    71821 accgctgacg aatccccaaa ccgcattacc gttgtagccg aattccgcac cggcaatcat
    71881 cgccacaccg gcaactgccg atattaagcc tgcctttttc agcccgctca aacgctcgcc
    71941 gaaaaagaaa acaccgattg ccgacaagaa aaagatttgc aggctgttga gcagggtgga
    72001 aacacccggc ccgaccgcgt gtatgctttc gtgccacaac gccaaatcga aagcgagaaa
    72061 cacgcccgcc gtcagggcat atcggacggt tttcctgttt tttgggaatt tttgcccgaa
    72121 aaaccgtgct aaaaaccaaa atacgaacac cgaaatcagc aaccgccaaa atgcgattgc
    72181 atacgaaccg acggggacgg atctgacaat cagactgccc agaccgaaca ccacgcaacc
    72241 cagcaccagc aatggtgcgg catatttttt gccgttaccg accacaatat cccctccctt
    72301 ccccatacgg ctgccgcgcc gacggcaaga atgccgccat tccgaaaata tgccgtctga
    72361 agcccaaagc cccggctgca cccggacaaa cggctgattt tacgggcagg tcggctattt
    72421 tccgttgggg caaaaaaccg tcctgccgga taaaaccc
//
LOCUS       DAWFIE010000003        63664 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWFIE010000003 DAWFIE010000000
VERSION     DAWFIE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 63664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63664
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.003"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            108..1074
                     /locus_tag="WGQ71_000153"
                     /pseudo
     CDS             108..1074
                     /locus_tag="WGQ71_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
     gene            complement(1121..3310)
                     /locus_tag="WGQ71_000154"
     CDS             complement(1121..3310)
                     /locus_tag="WGQ71_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ0706238.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSYSEPLPPGTRVLVPFRNKTVVGIV
                     WETDISPDMDTARILSVQTAFSDEPPLPQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     VKLIENWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRVRQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADALEFLNAAKETLAPLLPASVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            3449..4231
                     /locus_tag="WGQ71_000155"
     CDS             3449..4231
                     /locus_tag="WGQ71_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ0706239.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAAKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(4647..6581)
                     /locus_tag="WGQ71_000156"
     CDS             complement(4647..6581)
                     /locus_tag="WGQ71_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ0706240.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(6647..7825)
                     /locus_tag="WGQ71_000157"
     CDS             complement(6647..7825)
                     /locus_tag="WGQ71_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ0706241.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQASYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTTQTNTLNMEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAIPVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMKDSMNTEVKSGLKEGDKVVISEMTADEQQESGVRVMGGPPRR
                     "
     gene            8051..8440
                     /locus_tag="WGQ71_000158"
     CDS             8051..8440
                     /locus_tag="WGQ71_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0706242.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFDKYDKNKLKQNKYFNITLSEVSPDHFTLQAAPDPMTNE
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            8717..9763
                     /gene="adhP"
                     /locus_tag="WGQ71_000159"
     CDS             8717..9763
                     /gene="adhP"
                     /locus_tag="WGQ71_000159"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ0706243.1"
                     /translation="MKMQAVVVNKNVAGDVEVIEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVNADYAVKVPDGLDPAQASSITCAGVTCYKAVKV
                     SGVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(10114..13599)
                     /gene="smc"
                     /locus_tag="WGQ71_000160"
     CDS             complement(10114..13599)
                     /gene="smc"
                     /locus_tag="WGQ71_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ0706244.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIHQE
                     QQQIRVKLEENESQTEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPHGFVPPEPLPQGQAAWL
                     SDDLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELEKLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIVTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIAQLEQQTLDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQTLDEEYIVVQEKLAQIQEQGREQYAKVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNSKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCRLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            13848..14570
                     /gene="lpxH"
                     /locus_tag="WGQ71_000161"
     CDS             13848..14570
                     /gene="lpxH"
                     /locus_tag="WGQ71_000161"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ0706245.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAARQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQNFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLATKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFGAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            14839..16425
                     /locus_tag="WGQ71_000162"
     CDS             14839..16425
                     /locus_tag="WGQ71_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0706246.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(16594..16797)
                     /locus_tag="WGQ71_000163"
     CDS             complement(16594..16797)
                     /locus_tag="WGQ71_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ0706247.1"
                     /translation="MDNLNTQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRFDGKMPHHHYA"
     gene            16913..17410
                     /locus_tag="WGQ71_000164"
     CDS             16913..17410
                     /locus_tag="WGQ71_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ0706248.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            17492..18685
                     /locus_tag="WGQ71_000165"
     CDS             17492..18685
                     /locus_tag="WGQ71_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0706249.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVALLKAKG
                     ASQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(19089..20024)
                     /gene="hemC"
                     /locus_tag="WGQ71_000166"
     CDS             complement(19089..20024)
                     /gene="hemC"
                     /locus_tag="WGQ71_000166"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ0706250.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            20143..20658
                     /gene="ybeY"
                     /locus_tag="WGQ71_000167"
     CDS             20143..20658
                     /gene="ybeY"
                     /locus_tag="WGQ71_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ0706251.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KSGYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPEQHFAHLTIHGTLHLMGYDHIEDDEAEIMEAEEIRLMRAA
                     GFPNPYQEDGH"
     gene            20660..21484
                     /locus_tag="WGQ71_000168"
     CDS             20660..21484
                     /locus_tag="WGQ71_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ0706252.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVIIGSLQFTVARADNRRLHTLMATRVK"
     gene            complement(21627..23006)
                     /gene="nhaC"
                     /locus_tag="WGQ71_000169"
     CDS             complement(21627..23006)
                     /gene="nhaC"
                     /locus_tag="WGQ71_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ0706253.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLVRGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFHADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GAIPSLLDAVRSFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(23297..24520)
                     /locus_tag="WGQ71_000170"
     CDS             complement(23297..24520)
                     /locus_tag="WGQ71_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ0706254.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            25120..25533
                     /locus_tag="WGQ71_000171"
     CDS             25120..25533
                     /locus_tag="WGQ71_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0706255.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEDAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            25579..26208
                     /gene="nth"
                     /locus_tag="WGQ71_000172"
     CDS             25579..26208
                     /gene="nth"
                     /locus_tag="WGQ71_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ0706256.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            26346..27845
                     /locus_tag="WGQ71_000173"
     CDS             26346..27845
                     /locus_tag="WGQ71_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ0706257.1"
                     /translation="MFKKYQYLALAALCAASLAGCDQAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            28045..29565
                     /locus_tag="WGQ71_000174"
     CDS             28045..29565
                     /locus_tag="WGQ71_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ0706258.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAIITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDGSRGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVDPSLIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            29622..30707
                     /gene="nagZ"
                     /locus_tag="WGQ71_000175"
     CDS             29622..30707
                     /gene="nagZ"
                     /locus_tag="WGQ71_000175"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ0706259.1"
                     /translation="MTVPCIPRGLVMADIAAFRLTEEEKQRLLDPAVGGVILFRRNFQ
                     NIAQLKELTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHRNPEAVARLALAL
                     QKGLEKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLSELEAADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTTD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            30788..30864
                     /locus_tag="WGQ71_000176"
     tRNA            30788..30864
                     /locus_tag="WGQ71_000176"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30822..30824,aa:Pro,seq:cgg)
     gene            complement(31201..31839)
                     /locus_tag="WGQ71_000177"
     CDS             complement(31201..31839)
                     /locus_tag="WGQ71_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ0706260.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(31836..32207)
                     /locus_tag="WGQ71_000178"
     CDS             complement(31836..32207)
                     /locus_tag="WGQ71_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ0706261.1"
                     /translation="MKLLFILLVLFVAVEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QKRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            complement(32476..32898)
                     /gene="queD"
                     /locus_tag="WGQ71_000179"
     CDS             complement(32476..32898)
                     /gene="queD"
                     /locus_tag="WGQ71_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ0706262.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDPVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGG"
     gene            complement(32905..33423)
                     /locus_tag="WGQ71_000180"
     CDS             complement(32905..33423)
                     /locus_tag="WGQ71_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ0706263.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQIVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            complement(33451..34113)
                     /gene="queC"
                     /locus_tag="WGQ71_000181"
     CDS             complement(33451..34113)
                     /gene="queC"
                     /locus_tag="WGQ71_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ0706264.1"
                     /translation="MSNQQALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGIKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGGCRECPSCILRERGLAEYLESKK
                     AV"
     gene            34218..34643
                     /locus_tag="WGQ71_000182"
     CDS             34218..34643
                     /locus_tag="WGQ71_000182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706265.1"
                     /translation="MPSEHAAYADLTASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGAAVLGIVEDEDFEGDLGSWADSVKM
                     VRSSNGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            34658..35884
                     /locus_tag="WGQ71_000183"
     CDS             34658..35884
                     /locus_tag="WGQ71_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ0706266.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTRADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(36266..36400)
                     /locus_tag="WGQ71_000184"
                     /pseudo
     CDS             complement(36266..36400)
                     /locus_tag="WGQ71_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(36399..36857)
                     /locus_tag="WGQ71_000185"
     CDS             complement(36399..36857)
                     /locus_tag="WGQ71_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706267.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(38182..39117)
                     /locus_tag="WGQ71_000186"
     CDS             complement(38182..39117)
                     /locus_tag="WGQ71_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ0706268.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPESIDYVDEKKGVP
                     LAAMKYILMYTEAAASHAYLFEHDLKKFKQYAYVAGKLGILQSVDDEDPEPFFFPCDM
                     LNIQDPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDHEIAPRDLTVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
     gene            complement(39120..41261)
                     /locus_tag="WGQ71_000187"
                     /pseudo
     CDS             complement(39120..41261)
                     /locus_tag="WGQ71_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
     gene            complement(41368..41631)
                     /locus_tag="WGQ71_000188"
     CDS             complement(41368..41631)
                     /locus_tag="WGQ71_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706269.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            complement(41650..47259)
                     /locus_tag="WGQ71_000189"
     CDS             complement(41650..47259)
                     /locus_tag="WGQ71_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0706270.1"
                     /translation="MNKGLHRTVFSKKHNTMVAVAETANSQGKGKQAGTSAPASPQIS
                     DGLYSKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDNKGAVLNNDRNNNPFLVKGGAQLILNEVRGTASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGGADYTEILARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADS
                     TEASPTYLSIETTETGAAGAFISNGGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSTGGRTTINDATIQAGSSVYSSTKGDTELGE
                     NTRIIAENVTVLSNGSIGSAAVIEAKDTAHIEAGKPLSLETSNVASNIRLNNGSIKGG
                     KQVVLMADDDIQAKASHLNASGNLYIHAGSLSVRQSNLQSSGGNVQMSATKGNISLNQ
                     SWINASQNIDTAALQGNIISDGLTAVAEVGRVSLLANGNVDFNGLNTLIAEGDINAGS
                     VGKGRLKMDNTDIYASAGDVKLVAGGQLDLGNGTVNGGHISLDSNKGSMVVQNVHLNA
                     RASLKVDADQTLTINNSKLNSDHNTQINTNHGHMTLNQLDAHSRRHMSISAQGKGKGK
                     DSGQILQNDQQNSKSTLAADGVLSLNSSALQVLDNTTLRGGAINIKAGGGIIKRGHID
                     WETQDTATMRSAELKPLSGMMSIESGGDNPLTVEPGNRIVSAGDLAVKHNGTFQISAR
                     AGNNGNPSAQTASVSAKGNIGIVAGEVDIDAANIAAGKDLALVATKGNISLNSIRNTF
                     SNYQLKTDKHNITQQLTDVEQELSKLTSDPKYRKAQDLPQMLRRKYKRRDKVFGDSEA
                     RLRGLRAKINAADEAWAERQSPVKALLERKQLLQQALLTVSQPGSGHENQGSTLSGQN
                     IKLLAAGGIRIQGSKVAATQQANIQAAGFLPAPAAEELQEGRLQSAIDISGVLDTFEY
                     GQQGSDKYGYAIFSRPSEISGKTGVTLSAPNANENSRISLSAANIEAENGKIKIQSYG
                     DQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQGIDIKSGG
                     SIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYSKAHDTTT
                     QVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARADAKIILEG
                     IKTTIHNETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYIADIPKGN
                     LKTEIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGLTEAGAAIIALAVT
                     VVTSGAGTGAVLGLNGAAAAATDAAFASLASQASVSFINNKGDVGKTLKELGRSSTVK
                     NLVVAAATAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNL
                     EANILAALVNTAHGEAASKIKQLDQHYIVHKIAHAIAGCAAAAANKGKCQDGAIGAAV
                     GEIVGEALVKNTDFSRMSATEIEKAKAKITAYAKLVAGATVGVTGGNVDVAANASETA
                     VKNNALDIIWDIGNLVWDGGKWIYAKSIGDKQMAREAAIDFGVDAAAAAVPFVPAGAT
                     KISRGRAYVLKAGDEAVDTAKAIQEIQKQTGIKLTYDKVNKVWTTPAGLDYGLDAKHG
                     NRIKHVLAHTIPNPNKPVHSVFNVSRKEVLPLVDEAWRMKGNPLPNDSSVYLVDMKKP
                     IGTKGETKVRIVVQKGTNKIISAYPQK"
     gene            complement(47352..49094)
                     /gene="hrpB"
                     /locus_tag="WGQ71_000190"
     CDS             complement(47352..49094)
                     /gene="hrpB"
                     /locus_tag="WGQ71_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ0706271.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLPSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRLHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGKRLAGAVAGFRGSHKVGGIFAYDLFAGKPLHKPEGFQ
                     TANTVYGFNLNYSF"
     gene            complement(49155..49403)
                     /locus_tag="WGQ71_000191"
                     /pseudo
     CDS             complement(49155..49403)
                     /locus_tag="WGQ71_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(49687..49989)
                     /locus_tag="WGQ71_000192"
     CDS             complement(49687..49989)
                     /locus_tag="WGQ71_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706272.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            complement(50299..50793)
                     /locus_tag="WGQ71_000193"
     CDS             complement(50299..50793)
                     /locus_tag="WGQ71_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ0706273.1"
                     /translation="MRSEQIIRAGRSGYIAIPNVEVGQQVDPSKLLLSIVPERTELYA
                     HLYIPSSAAGFIKPKDKVVLRYQAYPYQKFGLASGSVVSVAKTALGRQELSGLGMVSS
                     DLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADILHEKRRLYEWVLELIYSM
                     SGKL"
     gene            complement(50858..51574)
                     /locus_tag="WGQ71_000194"
     CDS             complement(50858..51574)
                     /locus_tag="WGQ71_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ0706274.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQELH
                     ISQQIDGQKRRIRLAEEMLQKYRFLSANDAVPKQEMMNVKAELLEQKAKLDAYRREEV
                     GLLQEIRTQNLTLASLPQAA"
     gene            complement(52128..53282)
                     /locus_tag="WGQ71_000195"
     CDS             complement(52128..53282)
                     /locus_tag="WGQ71_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0706275.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDIRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHLA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPINYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKHEEVRKLIYQLIELDFLELL"
     gene            53644..53937
                     /locus_tag="WGQ71_000196"
     CDS             53644..53937
                     /locus_tag="WGQ71_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706276.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQAVGQRSDGIPANHTKEKP"
     gene            53934..54977
                     /locus_tag="WGQ71_000197"
     CDS             53934..54977
                     /locus_tag="WGQ71_000197"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ0706277.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSNPETVLRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLDAIPLLP"
     gene            complement(55802..57844)
                     /gene="recG"
                     /locus_tag="WGQ71_000198"
     CDS             complement(55802..57844)
                     /gene="recG"
                     /locus_tag="WGQ71_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ0706278.1"
                     /translation="MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQVREIAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(57930..58373)
                     /gene="secB"
                     /locus_tag="WGQ71_000199"
     CDS             complement(57930..58373)
                     /gene="secB"
                     /locus_tag="WGQ71_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ0706279.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDADLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(58394..58651)
                     /gene="grxC"
                     /locus_tag="WGQ71_000200"
     CDS             complement(58394..58651)
                     /gene="grxC"
                     /locus_tag="WGQ71_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ0706280.1"
                     /translation="MQTVTMYTGPFCPYCTMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            58865..60358
                     /gene="rng"
                     /locus_tag="WGQ71_000201"
     CDS             58865..60358
                     /gene="rng"
                     /locus_tag="WGQ71_000201"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ0706281.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(60393..61643)
                     /locus_tag="WGQ71_000202"
     CDS             complement(60393..61643)
                     /locus_tag="WGQ71_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ0706282.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQPAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVRGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGHL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(61656..61813)
                     /locus_tag="WGQ71_000203"
                     /pseudo
     CDS             complement(61656..61813)
                     /locus_tag="WGQ71_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            complement(62036..63349)
                     /locus_tag="WGQ71_000204"
     CDS             complement(62036..63349)
                     /locus_tag="WGQ71_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ0706283.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT        14859 a        17328 c        15786 g        15691 t
ORIGIN      
        1 tgcggccgtt tttcgtcggt gcggcggtac ttgccatact cggcgcgttg gtgtttttca
       61 tcagccccga tgccgtcgtc ctgcaccgcc aaatcttctt agaactcatg ctacctgcgg
      121 catatggtgg tttcctgact gcggctttgt tggactggac ggtttttttc gggtaacctg
      181 aaacctgtcg cgactttgat ggcggcatta ttgctcgccg catccgctat actgcccttt
      241 tcgccgcaaa ctgcctcgtt tttcgtcgcc gcctattggc tggtattgct gctgttctgc
      301 gcccggctga tttggctaga ccgaaacacc gacaacttcg ccctgctaat gttacttgcc
      361 gcgttcactg tttttcagac ggcatatgcc gtcagcggcg atttgaacct gttgcgcgcg
      421 caagtgcatc taaatatggc ggcggtgatg ttcgtatccg tgcgcgtcag tattcttttg
      481 ggcgcggaag ccctgaaaga atgccgtctg aaagacccag tattcatccc caatgtcgtc
      541 tataaaaaca tcgccattac cttcctgctc ctgcacgccg ccgccgaact ttggctgcct
      601 gcgcaaaccg ccggttttac ctcgctcgcc gtcggcttta tcctgcttgc caagctgcgt
      661 gagcttcacc atcacgaact cctgcgcaaa cactacgtcc gcacttatta cctgctccaa
      721 ctctttgccg ccgcaggcta tttgtggaca ggcgcggcga aattacaaaa cctgcccgcc
      781 tccgcgcccc tgcacctgat taccctcggt ggcatgatgg gcagcgtgat gatggtgtgg
      841 ctgactgccg gactgtggca cagcggcttt accaagctcg actacccgaa actctgccgc
      901 atcgccgtcc tcctgctctt tatggctgcc gtctcgcgtg ctttcttgat gaacgtgaac
      961 ccgatattct tcatcaccgt tcctgcgatt ctgaccgccg ccgtgttcgt gctttacctg
     1021 ctgacattcg taccgatctt tcgggcgaac gcgtttacag acgatccgga ataaaaatgc
     1081 cgtctgaaac ggttttcaga cggcatcgta ttgccgataa tcaagcctcc tgcggatcga
     1141 catccaccga ccatctaatt ttgccgtcgc ggttttgctg caacacctgc acccacaaac
     1201 tcacggcacg gtgcaaatcc tgtcgggacg gggattcgag gaagatttgc gcgcgttcgc
     1261 gttcggcgag gcgcaccatc agcatggggg cggcgccaaa ctgggaaacg cttgcgggca
     1321 acagcggggc gagggtttct ttggcggcat tgagaaattc cagcgcatcg gcaacacgcg
     1381 gcgcgtccgc gcggacggcg gtctgaaaac cgaagggcgg catggcgaac atttgccgtt
     1441 cgttcaattc gttttcggca aacacggcat aatcctgcgc tttgacggcg gcgaagacgg
     1501 gatgttcggg cagttgggtc tgtatcaaca ccttgccggg tttgtcggcg cgccccgccc
     1561 tgccggacac ctgcatcagc tcggcgaaca gtctttccgg cgcgcggaaa tccgcgctgt
     1621 aaaggctgcc gtcggcgttc aatacgataa cgaggttgag ccgcgcaaag tcatggcctt
     1681 tggcgagcat ctgcgtgccg accagaatgt cgatttcgtt gtcggcgatg cggcggtaca
     1741 aatccgccca gtcgtttttg tgcgcggtgc tgtccctgtc cacgcggacg acggctgcct
     1801 tgggcaggaa ggcgcgcagg gtttcttcga cgcgctgcgt gccgtgtccg acggcggtca
     1861 ggtcttgatt tccgcagtcg gggcatttga acggtatggg ttcgcggtgg tcgcagtggt
     1921 ggcagcgcag ttggcggacg cgttggtgca gcaccatttt ggcggagcag ttcgggcagc
     1981 cgaaggtatg gccgcagtcg ccgcaaaaca gcgcgggcgc gaagccgcga cggttgaggt
     2041 acaccagcga cataccgccc gcttcaaaat tctgtttcaa aagctgcaag gcttgcggcg
     2101 agaagccatt gtctagtttc agacggccta cgttgaggat gtccacttgc ggcagttgcg
     2161 cagcggtatg ggcgcgctcg gtcagttgca gcaggcggta cgcgccactt tgcgccttgt
     2221 gccagctctc caagctgggc gtggcactgc ccaacacgac ggggcagccg ccctgcttcg
     2281 cccgccacac cgccaaatcg cgggcatgat agcgcaattc gttgtcttgt ttgaacgagc
     2341 cgtcgtgttc ctcatcgacc acaatcagcc cgacatcatc catcggcgtg aacactgcca
     2401 gccgcgtgcc gatcaccagc ttcgcctgcc ctaacatcgc gcgcagataa tcctgcgtgc
     2461 gcttgcctgc cgccatctga ctgtgcaaca cggcggtcgg cacgtcggca aaacggtttt
     2521 ccacccgctt caaaagctgc ggcgtgaggt tgatttcggg caacagaaac aacacctgcc
     2581 gcccctgagc caacactttc gccatcgcat caaaatacac ctcggtcttg ccgctgccgg
     2641 tgatgccgta cagcagaaac ggctggaagc gtcccaatgc cgtctgaatg gcatcggaag
     2701 ccttctgctg gtcagcattt aatacaaatt cagagtgcga agcctgccca ttacacgacc
     2761 ttaaaacggg cttcgccgct tccgtcgttt caatccaacc ctgttccgcc caattttcga
     2821 ttaatttcac cgcctgcgcg tttacctgct tcaacgctgc catcgtcatt tcgcccgaca
     2881 gcagcgcgtc ccacaaagcc gcttttttgt tgaaccgcgc cggcggcggc gtttgcgccc
     2941 tgcctgcttc gtttaaagca taaaacaatg gcggctgcgg catttccacc gcgcgcgttt
     3001 ccttcaaacc ctgcggcagt gcggcaaata ctgcctgccc cgtcggatag tggtaataac
     3061 gcgacgtaaa cgccaacaaa tcacgccagc tttgaggcaa cggcggttcg tccgaaaagg
     3121 ccgtctgaac gctcaaaatc cgcgccgtat ccatatcggg cgaaatatcc gtttcccaca
     3181 caatcccgac aacggtttta ttgcgaaaag gcacaagcac ccgagttccc ggaggaagcg
     3241 gctcggaata ggaatacgtc aaaaggccgt ctgaaagcgg cacgtttacg gcgatgcggt
     3301 ggtagatcat acaggcaggc aaatcatttt tttgcgtatt ttaacaggcg gaagctattt
     3361 tcagacgata aattgagcct aggcgcaatg caggcgcaac agagatttga tacaattccg
     3421 cctttccaac acgcgcaatc atctttatat gaaaaccaag ttaatcaaaa tcttgacccc
     3481 ctttaccgtc ctcccgctgc tggcttgcgg gcaaacgccc gtttccaatg ccaacgccga
     3541 acccgccgcc aaagccgagt ccgccggcaa atccgttgcc gcctctttga aagcgcgttt
     3601 ggaaaaaacc tattccgccc aagatttgaa agtgttgagc gttagcgaaa caccggtcaa
     3661 aggcatttac gaagtcgtcg tcagcggcag acagattatc tacaccgatg ccgaaggcgg
     3721 ctatatgttc gtcggcgaac tcatcaacat cgacacgcgc aaaaacctga ccgaagaacg
     3781 cgccgccgat ttgaacaaaa tcgacttcgc ctccctgcct ttggacaaag ccatcaaaga
     3841 agtgcgcggc aacggcaagc tgaaagtcgc cgtcttctcc gaccccgatt gtccgttctg
     3901 caaacgcttg gaacacgagt ttgaaaaaat gaccgacgtg acggtttaca gctttatgat
     3961 gcccattgcc ggcctgcacc ccgatgccgc gcgcaaggcg caaatcttat ggtgtcagcc
     4021 cgaccgcgcc aaagcgtgga cggattggat gcgtaaaggc aaattcccgg tcggcggcag
     4081 catctgcgac aatcccgtcg cggaaaccac ttccttgggc gaacaattcg gcttcaacgg
     4141 cacgccgacc ctcgtcttcc ccaacgggcg cagccaaagc ggctacagcc cgatgcccca
     4201 actggaggaa atcatccgca aaaatcaata atccatccgc aaaacaaaag gcttggaaaa
     4261 ttccaagcct tttttgcggg aaatgccgcc tgaaaaccaa tccgccctga aagttagcaa
     4321 gccaacaaac acatcctaac ccattgaatg attgaaaata ataaattgaa caatacgaag
     4381 ccccgccgcc gttcccacgc gggcgggaat ctggaatatt gagttaccgt gatttatccg
     4441 aaacaagcaa agccgaacga accgaattcc cgtctttacg ggaatgacgg gactcgagcg
     4501 ttgtgtgcaa tcatcgaacc cccgccgccg tcattcccac gcaagtggga atctagaacg
     4561 tagaatctaa gaaaccgttt tgtccgataa gtttctgctc caaaatgacc ggtccggcag
     4621 cttcaaacgg catctttgtg ccaacctcaa tcctgcgcca atgcgtctat cggattgagt
     4681 ttggctgctt tattggcagg cataaagccg aacgcgatgc cgattccggt cgaacaggcg
     4741 accgccccga taacggatgc cgccgaaatg tccatcggga agtcggttac aaaatgattg
     4801 aacacgaggc tgacggcggc ggacaaaccc acgccgacca aaccgccgat gacgcagatt
     4861 aacaccgcct caatcaaaaa ctgctgcaag atattgccgc gccgcgcgcc gattgccatc
     4921 cgtatgccga tttctttggt gcgctcggta acggacacca gcatgatgtt catcacgccg
     4981 atgccgccga ctaccaatga aatcagggcg atggaggaaa tcagcagctt catcgtaccg
     5041 gtggtgcttt cgactatctg cctgatgctg tcgctgttgt tcatgaagaa atcttccgtg
     5101 ccgtgccgcg ctttgagcag atcggtcagc cctttttcgg caacctgggt attggcattg
     5161 tctttgattt tgacggtgat ggagttggtg tggctctcgc ctgtgatttg gtgcatcacc
     5221 gtcgtatagg gcgaccaaag catcagcacg tcggaattgc cgaaagcgtt ttcgtctttt
     5281 ttcatcacgc cgatgacggt caaggggcgt ttcctgaaca aaatggtttt acccaacgga
     5341 tccgagtccg caaagagttt gtctttgaca ttttggtcga tgacgacgac ctgcgcgtct
     5401 tctttcacat cgttttcgtc aaacagccgc cccgtttcca gcttcagtcc gcgcacgtcg
     5461 aaatattgtt cgcccacgcc gtacaaagaa gcggtcaggt cggtattgcg gtaagtcagc
     5521 gtgccgccgc tcgaagtcat gggcgtggcg gaagcaacgt agctttgttt ggcgatgatt
     5581 tttgcgtcgt ctatggtcag ggttttaatc ctgccgctgc gcctgtcgcc gaagccgcgc
     5641 cccgggaaga tgctgatggt gttcgtccct atcgaactga tgtcttcaag gatttttttc
     5701 tgcgaaccat tgcccaatgc gacgacggag acaaccgaag cgataccgat gatgatgccg
     5761 agcatcgtca gaagcgaacg cattttgtgc gccaatactg cttgcaccga cattctgaag
     5821 gcttcgacaa actggtcgta ataaaacgac cacgaggctt tttcctgaat cctcccgaca
     5881 ttgcttgcgg ggatttctgg atttttcgag gtgtcggaaa tgatttcgcc gtcccggatt
     5941 tcgatgacgc ggttggcatt ggcggcgatg ccggggtcgt gcgtgaccat aatgacggta
     6001 tgcccggctt cgtgcagcct gcggatgatt tccatcacgt ttttgccgct ggcggtatcg
     6061 agcgcgccgg tcggctcgtc ggcgaagatg atttctccgc cgttcatcag ggcgcgggcg
     6121 atggagacgc gctgctgctg tccgcccgag agttcgccgg gcttgttgcc ctctttgctt
     6181 gccaaaccca aatcctgcaa gagtttgtcc gcccgcgcgg aacgctcttt gccgcccgcg
     6241 cccatataga cggctggcag cgcgacgtta tcccttgcgg tcagcgagct taagaggttg
     6301 tagcgttgga agataaagcc gaagcgttcg cggcgcaatg ccgccagctc gtcaggctgc
     6361 attttggcag tttcgatgcc gtcgattcgg tacgaaccgg aaccggcggt atccaaacag
     6421 ccgagtatgt tcatcagcgt ggacttgccc gaaccggact gcccgatgat ggcgacaaaa
     6481 tcgcccttct ctatcgacag gctgatgtct ttcaaaatat ggacgcggtt ctcgccgctg
     6541 ccgaaatagc ggttgatgtt tttacattcg atcaagctca taatattccg taaatcaata
     6601 acaaatgccg tctgaaaccg tttccgtgtt cagacggcat attcgtttat cggcgcggcg
     6661 ggccgcccat cacgcgcacg ccgctttcct gctgttcgtc cgccgtcatt tcggagataa
     6721 ccactttgtc cccctctttc aaaccgcttt tcacttcggt attcatactg tctttcatac
     6781 cggttcggat ttcgcgttcc gccgccttgc cgtccgcacc caacacgcgc acaaacgcct
     6841 tgccgccgcg atttttcacg gtcagcgacg gaataatcag cacatttttc acgccgtcga
     6901 tttcaaccgt attctgcgtc gtcatccccg tggcgagttt gccgtccgga ttcggcacaa
     6961 acgaacgggc ataatagtag accgcattgg aagccgtatc cgtactgctg ttgtagccgc
     7021 ccgacgacat cgtggtcagc ccggggtcga cgctatcgag cttcgcctta atcggcgtat
     7081 ccggttcgga caaaatcgta aacgaaatgt cctgccccgc ctttaccttg gtaatatcgc
     7141 cctcggcaat ctgcattttg ttcaacatca tatccagatt cgccaattgg acaatcgtcg
     7201 gcgtagactg cgccgcgttc acagtctgcc cctcttccac gggaatcgcc accaccgtgc
     7261 cgtccatcgt cgcggtaatg cgcgtgtagc ccaattccga ctcggcggta ttgatggaaa
     7321 ttttgctctg tctgattaga gccttcagct cggcaacatt ggctttggcg gcggcaagcg
     7381 catcctgcgc gctttccaaa tcttctttag aggtcgcatc atccttccat aacgccgcct
     7441 ggcgtttgta tttcttctcc gcactgccca atgcaatctg tgccgacacc agcttcgcct
     7501 gatacgtttc caatttggat ttttccatat tgagcgtgtt agtctgcgtg gtcgaattga
     7561 tttccgcaat caaatcgccc tttttgacct gttgtccgag tttgacataa agtttcttaa
     7621 tctgccccga tgcctgcgcg ccgaccgata ccagattcga cggcgaaatc tcccctgtcg
     7681 cagaaaccgt ccggctgatg tcgccgcgcc tgaccgtttc cgtaatatag gaagcctgcg
     7741 gttcgggctt cagataagac catccgcccc aaaccgctgc cgccgcgacc gccgcaacag
     7801 ccgcccattt catcattttt gccatgtttt caattctctg tatgcgtcaa tcccataaat
     7861 cggaagcgga ttctaccgca agtccctgcc ccatccaagg cggcaaactg ccgacaataa
     7921 aaataaccat ttgttttaaa tggcttatac aatctgcttt cccgaaaatt tcaatccgtt
     7981 tacatattcc gccatttccg atacggcgtt ataatgccgt cgaccgacaa acaaatggaa
     8041 acacattgct atgaaaaacg ttcaaaaagg ctttacgctg ctcgagctga tgattgccgt
     8101 cgccatcctc ggcatcctga cgcttatcac ctacccttcc tataaaacct acatccggcg
     8161 cgtccgcctg tcggaagtca ggacgaccct gctgcacaac gcgcagacca tggaacgtta
     8221 ctaccgccaa aaagggacgt ttgacaaata cgacaaaaac aaactgaaac aaaacaaata
     8281 tttcaacatt accttaagcg aagtcagccc cgaccacttc acccttcagg ccgcccccga
     8341 tccgatgacc aacgaaggcg aaacctgcgt cgttacgctc aacgacggcg gtaccattgc
     8401 cgcttccggt acaaaccaat cctgccccgg cttcgactaa tcctccgccg aagcccgcaa
     8461 acccgtaaag atgccgtccg gacacagctt cagacggcat ttttacgggt ttgcccatcc
     8521 ggtttttcaa acttgaaacc tgccgccccg gcaaccgccc agatgccgcg ccttttcaag
     8581 ccgttatagt ataacatata acccatattt ttcaacaaca taccatttga cctgtaagat
     8641 gcggaagcgt atgcttaccc ttatatcgga caacattccg tttacaggtt ttttaccgta
     8701 actaaggagc gcaatcatga aaatgcaggc agttgttgtg aataaaaatg tagcgggcga
     8761 tgtggaagta atcgaacgcg aggttcgccc gttggaatac ggcgaggcat tggtcgaagt
     8821 cgaatattgc ggcgtgtgcc acaccgacct gcacgttgcg gcaggcgact acggcgaaaa
     8881 accgggccgc gtgttgggac acgaaggcat cggtttggtt aaagaagttg ccgacggtgt
     8941 gaaaaatctg aaagtcggcg accgcgtcag catcgcctgg ctgttccaaa gctgcggctc
     9001 ttgcgaatac tgcaataccg gccgcgaaac cctgtgccgt tccgtattga acgcgggcta
     9061 caccgccgac ggcggtatgg cgacccactg catcgtcaat gcggattatg cagtcaaagt
     9121 ccccgacggt ctcgaccccg cccaagcgtc cagcatcact tgcgccggcg ttacctgcta
     9181 caaagccgtg aaagtatccg gcgtgcgtcc gggacagtgg attgccatct acggcgcggg
     9241 cggtttgggc aacttgggtg tccaatacgc gaaaaaagta ttcggcgcgc acgttgtcgc
     9301 catcgacatc aacgacgaca aactggcgtt tgccaaagaa accggcgcgg atttggttgt
     9361 caacgccgcc aaagaagacg ctgccaaagt gattcaggaa aaaaccggcg gcgcacacgc
     9421 tgcggtcgta accgccgtat ctgctgccgc attcaactct gccgtgaatt gcgtccgcgc
     9481 aggcggacgt gtggttgcca tcgggctgcc gccggaatcg atggatttgt ccatcccgcg
     9541 tttggttttg gacggcatcg aagtggtcgg ctctttggtc ggcacgcgca aagatttgga
     9601 agaagccttc caattcggcg cggaaggttt ggttgtgccg aaagtccaac tgcgtgcttt
     9661 ggatgaagca cccgccattt tccaagaaat gcgcgaaggc aaaatcaccg gccgtatggt
     9721 aatcgatatg aaaaaagaat gcggctgcgg ccatcaccac tgatttgacg tggcagtaca
     9781 catcgaaatg ccgtctgaac gctgttcaga cggcattttt tatggatttg gatttgattt
     9841 taatctgttc tgtttgaaat accgtctgaa aacccgtatc gcaacacgtc attatagtgg
     9901 attaacaaaa atcaggacaa ggcggcgagc cgcagacagt acaaatagta cgaaaccgat
     9961 tcacttggtg cttcagcacc ttagagaatc gttctctttg agctaaggcg agccaacgct
    10021 gtaccgattt aaatttaatt cactataccg tctgaaaacc catatcgcaa cacttcatta
    10081 aaaacggcaa gattcagccg ttctgcaacc cgttcaaacg gcttccgcca tttccaacgc
    10141 ctgtttgatg tccacggcga cgacgcgcga tacgcctttt tcctgcatgg ttacgccgac
    10201 cagctgctct gccatttcca tggtcaggcg gttgtgggag atgtagagga actgggtttg
    10261 cgccgacatt tctttgacca gcctgcagaa acgcgaggtg ttggcgtcgt ccagcggggc
    10321 atcaacttcg tccagaaggc agaagggtgc ggggttgagg ctgaacaggg cgaacacgag
    10381 gctcatggcg gtgagggctt tttcgccgcc ggagaggagg tggatggtgc tgtttttctt
    10441 gccgggcgga cgcgccatga tggatacgcc tgcggtcagg aggtcgtcgc ctatcatttt
    10501 gagggtggct tcgccgccgc cgaacagggt ggggaagaag gtttggactt tgctgttgac
    10561 ggcatcgaag gtttctttga aacgcgcttt ggttttgtcg tcgatttggg cgatggcttc
    10621 ttccaaaagg gtgatggctg cctgcacgtc ttcgctttgg ctgcggtagt agccgtcgcg
    10681 ttcgcgcgct tcttcgagtt cttgcagggc ggcgaggttg accgcgccga gtgcttcgat
    10741 ttgttgcgaa aggctgccga tgctgctgtt caatactttc ggcgattctt tcgccaacgc
    10801 ttcgagcgcg tccaaatcgg cggcgcgttc ggtcaggttt tgatggtagc gtttggcgtt
    10861 gatcagggct tcctgctgct gcaacaaggc ggtttgggtc gcggcctgaa gctgaggcag
    10921 cttggtttgc agggtttgca ctttagcgta ttgttccctg ccctgctcct gaatctgcgc
    10981 gagtttctcc tgtacgacaa tatattcttc gtccaaggtc tgtacggctt cgcttaattc
    11041 ttcaagcttg atgtgctgct cgtcgttttg gaactcggtt tcataggcga gggcaagctc
    11101 ttgctggcgt tcctgccagt ctagggtttg ctgttcgagc tgggcaattt gctgccggta
    11161 gttttgtttt tgctggttga gcttgtgaac ggcgacttcg gccagcccgt attggcggtt
    11221 ggcttccaac agggcaagct gcgcctgttt cagacggcct tgctgctctt ggcggctgtg
    11281 cgcggtggtt tgctgctggt gttcgagttc ggcggcggct tcctgcaagg taacgatgtc
    11341 gtctgaaagc ccgtcggacg tgtgttgcaa cacggtctgc tcttccgcca actgcgccag
    11401 ttcgcgctcg atgtgttcgc ggcggatttg cccttggttg gtgcgcgcca gaagttcggc
    11461 ggcgcgttgc tgcgcctgac tgtactggcg cgtatgctgc tgttgttgct gcatcaggtt
    11521 tttgtgctgc acttcggacg agcgcacggc ggcttccgcc tgtttgaacg cggcttcggc
    11581 ggcggagagt tcgggggcaa gtttttccag ttcggacgcg atgccatcga ggcgtgcttt
    11641 ttgggcaatc aggctttcct gcgcgggctt ggcatagagc aggacgctga ctttgtcgac
    11701 ctgatggcct tcgggcgtaa gccagatttg gtgtgcgccc aaatcgcttt gatgcgcgag
    11761 ggcgtagctc aaatcgggcg cacacaatac gccgtcgagc cagtggtgca atgccgtctg
    11821 aaacggcggc tgcgcttgga tttggttcag caatgcctgt acgggcaggg attttttgat
    11881 gccgcctgag aggtcgtctg aaagccatgc cgcctgcccc tgcggcaaag gttcgggcgg
    11941 tacgaagccg tgcggcacgg cgcgggcgtg caggcgttcg gcaaggatga cggacaaggc
    12001 gtgctgccac tcggcgggcg cggtgatgtg ttgccacagt tgcggcgcgg cggcgtggtc
    12061 ggttgcctgc cagaagtcgg cagcttcctg ctgttgcgat aggatttgcg acaacgcctg
    12121 ctgctgcgcc tgcaaggtga tgtgttgctg cttcaggctt tggaagcggt ttgaggccgt
    12181 ctgaaacgct tcgcgggcgg catgtagggc ttcttcggcg gcgatgattt gttcttcgta
    12241 atgctcttgc cggctttgca acaaggcggc ggcttcctgc gcggcggcgg tttcggcttc
    12301 gtcgggcagg ttcagggctt ggttttcctg tttcagacga cctttgcgct cttcgtgctt
    12361 ggcaacggtt tgttcggcat gggcaagctg ctgctgcttc aacgccaact cgcggcggat
    12421 gcggttcgcc tcgtcctgct gggtttggaa ggcggcgttg agcgtggctt gggcttcttc
    12481 caattcgggc agacgctctt cgtgttcggc aacctgcatc gcccattccg ccaattcggt
    12541 ttgcttttct tcggtctgcg actcgttttc ttccagcttg acgcggattt gctgctgctc
    12601 ttgatggatg cgttgcatct gcgcctgcgc cgcctgcttg tcgcgttcga tgcgttggtg
    12661 caggttttgc tgatggcgga tttgttcttc caaacgggca atctgctcgc gcaacacgcc
    12721 gcgtttgttg cccagttcgt gtaccgcctg ctgctgcgac tgttcggcgg tctgcaatgc
    12781 gtgtacttcg tcgtttaacg cctgaacctg cgcggcggtt tcgtcctgct gcgcctgcaa
    12841 agactgatgc tgcgcggtcg ctttgtcggc ggcggcaagc gattgctgcc attgggcgta
    12901 atcgagcaaa tcctgttgtt ggttcaactg cgcggtcagg gatttgtagc gttcggcggt
    12961 ttccgcctgt ttctccagct tttccacctg acgcgccaac tcgttctgca aatcgcccaa
    13021 acgctgcaaa tgctcgcgcg tgtctttcag acggccttcc gtctccttgc ggcgttcctt
    13081 atatttggac acgcctgccg cctcctcgat ataggcgcgc aactcctccg gccgcgcttc
    13141 gatgatgcgc gaaatcatcc cctgctcgat aacggcatac ccgcgcgcgc ccacgcccgt
    13201 acccaaaaat aaatcggtaa tgtcgcggcg gcgcacggtc tgattgttga tgaaataagt
    13261 cgattcgccc tgacgcgtca gctgccgctt gatgctcacc tcggcatact gcccccacgc
    13321 cccctgcaaa ctgtggtcgc tgttgtcaaa caccagctcc accgaagccc tcggcgcagg
    13381 acggcgcgtc gccgcaccgt taaagatcac gtcctgcata ctctcgccac gaagctgctt
    13441 cgccgaagcc tcgcccaata cccagcgcac cgcatcaatc acattcgact tgccgcagcc
    13501 gttcggcccg ataaccgcaa caagctgccc cggaacatga atcgtggtcg ggtcggtaaa
    13561 agatttgaag ccggagagtt tgatgtgggt caggcgcata atggtcggaa aaaataaaaa
    13621 agaagcgtat tttaacggaa atcccgccgc accaccccat atcttgccgg caaaacctta
    13681 ccgcatcccg cacccgcgat gccgtctgaa gccttcagac ggcattttta acgcgcccga
    13741 accccggttc cgccaatgcc cgatgcttgg cgcggcattt gccgtattgc cccaccgccg
    13801 ggcgcgttat aatccgcccc gacatccgcc cgattggaaa cctgcctatg aaacccgcct
    13861 atttcatttc cgacctgcat ttgagcgaaa agcagcccga actgaccgcg ctgttgctgc
    13921 gttttttacg ttcttccgcc gccaggcagg cgcgggcggt ttacattttg ggcgatttgt
    13981 ttgatttttg ggtgggcgat gacgaagttt ccgagttgaa tacttcggtt gcgcgtgaaa
    14041 tcaggaaatt gtccgacaaa ggcgttgccg tgttcttcgt caggggcaac cgcgacttcc
    14101 tgatcggtca gaatttttgc cggcaggcgg gcatgacgct gctgccggat tactcggttt
    14161 tggacttgtt cggctgcaaa accctcatct gccacggcga cactctgtgt acagatgaca
    14221 gggcatacca acgtttccgc aaaatcgtgc atcggaagcg gctgcaaaaa ctgttcctaa
    14281 tgctgcccct gaagtggcgc acgcgccttg ccaccaaaat caggcgtgtc agcaaaatgg
    14341 aaaaacaggt caagcccgcc gatattatgg atgtcaatgc cgcctttacc gcgcggcagg
    14401 tacgcgcctt cggtgcggaa aggctgatac acggacacac ccaccgcgag catatccatc
    14461 acgaaaacgg ctttacccgc atcgttttgg gcgactggca taacgactat gcttcaatcc
    14521 tccgcgtgga cggggacggc gcggtattcg tgccgctgga aaaatactga aaatgccgtc
    14581 tgaagccttt cgggcggctt tttttacacg cccctttccc acactccctc cgccgcatca
    14641 tcaccatcct taacccgcat taatagccat tatcgataaa atgcttgacc gattacataa
    14701 gattacgtaa agtgtgcaaa cgcataacaa ttggtcttac caatttatct tttaaccaaa
    14761 atatcgggtg tacgggattt gaacgtcttt ccaaacctcg acgcgcctta tgatgcaaat
    14821 gcctaaaaag gattcgttat ggcacttttc ctcagcatat tccccatcgt cctgctgatt
    14881 tggctgatgg tgaaaaaaaa cagtatgccc tcctacgtcg cgctgccgat taccgcagtg
    14941 ctgatttacg ccatcaaact tttctacttc gacgatgcgg gcatgctgct caacgccacc
    15001 gccgcttccg gcctcgtcaa aacgctcacg ccgattaccg tgattttcgg cgcgattatg
    15061 ttcaaccgta tgatggaaac cacgggctgc atcgatgtca tccgcaaatg gctggcgacc
    15121 atcagcccca accccgtagc gcaactgatg attatcggct gggcttttgc ctttatgatt
    15181 gaaggcgcat ccggcttcgg cacgcctgcc gcgattgccg cgccgattct gatgagcttg
    15241 ggcttcaacc cgttgaaagt ggcgattttc actttggtga tgaactccgt ccccgtctct
    15301 ttcggcgcgg tcggtacgcc gacctggttc ggtttcgcac cgctgaacct gagtgccgaa
    15361 gacatcctcg ccatcggcag gcagaccggc gtaatgcact tcttcgcagg tttcgtcatc
    15421 cccgtcatcg gcttgggctt catcgtacct tggtctgaaa tccgcaaaaa cttgggcttc
    15481 gtcgccattg ccgtcttctc ctgcaccatt ccttatgtcg cattggcgat ggtcaacgaa
    15541 gaattcccgt cgctcgtcgc cggcgcgatc ggcctgatgg tgtccgtatt cgccgccaac
    15601 caaggctggg gcttgagcaa agaccacgcc aaagacccga atgccgaaaa agtgccgttc
    15661 gcccaagtcg ccaaagcact cgcccctttg ggtatgctga tcggcatgct ggtggttacg
    15721 cgcatcaaac agctcggcat caaaggcatt ttgaccagca aagaagaatg gttcagcttc
    15781 caactgccgt ttgatttgtc caaaatcacc gtcagcgact ccctgacgat taccttcggc
    15841 aatattttcg gacaagatgt cagcgcgtct taccaaacgc tgtacgtccc ggcttggatt
    15901 ccgtttgtgc tgaccgtttg gatttgcatc ctgctgtata aaaccaaatt caaagatgcc
    15961 tggacgattt attccgtaac tttcaatcaa accaaaaaac cgctgcttgc cctgatgggc
    16021 gcgctgatta tggttcagct gatgctggtc ggcggcgaca attcgatggt gaaaatcatc
    16081 ggtaaggaat ttgccgcaat ggcgggcgaa cactgggttt atttctcgcc gtatctgggc
    16141 gcgatcggtg cgttcttctc cggctccaac accgtgtcca acctgacctt cggaccgatt
    16201 cagcagcaaa tcgccttgga taccggcctg tccgttaccc tgattctggc gttgcagtcc
    16261 gtcggcggcg cgatgggcaa tatggtgtgc ctcaacaaca tcatcgccgt atgtaccgta
    16321 ttggacgtga aaaattccga aggtgcgatt atcaagaaaa ccgttatccc gatggcgatt
    16381 tacggcgtga ttgccgtcgt cgcggcaatg attttcttcc tctaggcgaa acgccaccat
    16441 ccatagcggc agcacgcggg gaacggaaac cgccgttccc cgtccgggcg gcgcgtcggg
    16501 cagaatcccg gtgccggaac aagcccgccc cgccaaacaa atgccgtctg aaaccgaaaa
    16561 aggcttcaga cggcattttt ccgccgcttg cgttcaggcg taatgatgat gcggcatctt
    16621 gccgtcaaag cggtagcgcg tgccgcaata cgggcaggca acgctgcccg attctccttc
    16681 gcacaaaggc aaaaacactc tcggatgccc gttccactgc tcgttgtcgg gccccgagca
    16741 atacaacggc agattttccg gcaacacgga aatttcctgc gtattcagat tgtccattta
    16801 attttccttt gcttggttgg tatccgtcca ttttacgcca tcggcaggct aaaggatatt
    16861 ttcggcgcaa agccgcaatc cgctataatc cccacttttc agacggcata ccatgactgc
    16921 gcttaccctt ccggaagaca tccgccaaca agagccatcc gccctgctct ataccctcgt
    16981 ttccgcctac ctcgaacaca ccgcccaaac cggcgacgaa tccctctcct gcctgtccga
    17041 cgaccagcac acgctgaccg cattctgcta cctcgacagc caagtcgaag aaggcggctt
    17101 cgtgcaactc atcgcctccg gttacggcga atatattttc cgcaacccgc ttgccgacag
    17161 cctgcgccgc tggaaaatca aagccgtgcc gaaagtcttg gacaaagcca aagccctcta
    17221 cgaacaacac ggcaaaacca tcgaaacgct cgccgacgga ggcgcagaca tcgaatccct
    17281 gcgcaaacag ttccccgact ttgaagaatg ggacggcgca tactacgaag ccgccgaaca
    17341 agacctgccc ctgcttgcag aacacataca gtcaaactgg gaaaccttcg cccatatcgg
    17401 gcaggcgtga ttgcgtctgt ttccagcccg tgtaaaacag cgtaaaatcg gcaaacccga
    17461 atgaccttcc gtccccatca aggagcaagc tatgttcttc aagcacatcg aagccgcccc
    17521 cgccgatccg attctcggtt tgggcgaagc gttcaaagcc gaaacccgcc ccgaaaaagt
    17581 caacctcggc atcggcgttt ataaagacgc atccggcgcg acacccctcg tcaaagccgt
    17641 caaagaagcc gaaaaacgcc tgttggaaag cgaaaccacc aaaaactacc tcaccatcga
    17701 cggcgttgcc gactacaacg cgcaaaccca aatcctgctg ttcggcaaag accacgaaat
    17761 catcgccagc cgtcgcgcca aaacagcgca aagcctcggc ggtacgggcg cattgcgtat
    17821 tgcggccgag tttgccaaac gccagttgaa cgcgcaaacc atctggattt ccaatccgac
    17881 ttggcccaac cacaacgcca tcgccaaagc ggtcggtatc caagacaaac cttatcgtta
    17941 ctatgatgcc gccaaacacg gtttggattg ggacggcatg attgaggact tgagccaagc
    18001 gcaaaaaggc gacatcgtcc tgctgcacgg ctgctgccac aatcctaccg gcatcgaccc
    18061 tacgcccgaa caatgggaaa ctttggcaaa actttctgcc gaaaaaggct ggttgccgct
    18121 gtttgacttt gcctaccaag gcttcggcaa tggtttggaa gaagatgcct acggcttgcg
    18181 cgtgttcttg aaacacaata cagaattgct gattgccagc tcttattcca aaaacttcgg
    18241 tatgtacaac gagcgcgtcg gcgcgttcac tttggtggcc gaagatgaag aaacagcagc
    18301 ccgcgcccac agccaagtca aaaccatcat ccgtaccttg tattccaacc cggcttcaca
    18361 cggtgcgaac accattgcgc tggtgttgaa aaatgatgat ttgaaagccc aatggattgc
    18421 cgaactcgat gaaatgcgcg gccgcatcaa agccatgcgc caaaaatttg ttgcgttgct
    18481 caaagccaaa ggtgcaagcc aagactttga tttcattatc gaacaaaacg gtatgttctc
    18541 tttcagcggt ctgactcccg aacaagttga ccgtttgaaa aacgagtttg ccatttatgc
    18601 cgtccgctcc ggccgcatca acgttgccgg cattaccgac gacaacatcg attatctgtg
    18661 cgaaagcatc gtgaaagtac tgtaaaaagc acagccatat gaaaatgccg tccgaacctt
    18721 aaccagttca gacggcatct gttttttgtt aaaaaacaat aaattataac aatttcaaac
    18781 aagctaaaaa catctataaa atcaacccaa tatgatgaac tcaaataaaa aatacgaata
    18841 tcatcattct caaattcaaa atttgaacgc cacagtatta tccgaagcca caaaaacctt
    18901 tccgccgtca ttcccgcgca ggcgggaatc cgggaatcca atgctgcaag aattgattaa
    18961 aaacaactga aaccgagcaa actggattcc cgcctgcgcg ggaatgacga aataaggcga
    19021 gtaaaactga tgaaacaaat tgattaaaaa caataactta tattatttta tttaaatttt
    19081 cgataaaatc aattttccgt attcaatact gctccaatca attcccgcgc accgtcgtcc
    19141 gccaatttct tagcgacagc gcgtccgagc gcgtcggcat attcggcagg ggcttgcgcg
    19201 tccgcccgca acacaaccga accgtcggga tgaccgacca agccgcgcaa ggtcagcaag
    19261 ccgttttctt ccgtgcaata tgcggccagc ggcacttggc agcttccgcc caaagcgcgc
    19321 gccaaagctc gttcggccgt aacgcaggcg tgtgtggtat cgtggtttag cggttttaaa
    19381 acctcataca aatcttcgcg gtgcgcggca atttcgatac ccaatgcgcc ttgtccggcg
    19441 gcaggcaggc tgtcggattc cgacaaaatc atgcggatgc gtccgtccaa tttcagacgc
    19501 tgcaaaccgg cggcggccaa aataattgcg tcgtattcgc cgttatcgag tttggacaaa
    19561 cgggtttgca cattgccgcg caaaggtttg ataagcaaat gcggatagcg cgcgcgcaac
    19621 tgggcttcgc ggcgtaggct ggatgtaccg acgacagcgc ctttgggcat ttcttccaaa
    19681 cgcgcgtatt ggttggacac aaacgcgtca aacggattgg cgcgttcgcc gatggcggca
    19741 agcgcgaaac cttcaggcaa atccatcggc acgtccttaa tcgaatgcac cgccaaatcg
    19801 gcgcgcccgt catataaagc ctgttccaac tctttgacaa acaagccttt accgccgact
    19861 tttgacaaag ttttgtccaa aatctgatcg ccgcgcgtgg tcatgcccaa aatctcgact
    19921 tcgcaatcgg gatacagagc cttcagacgg ccttggatat gctttgcctg ccacatagca
    19981 agcaggcttt cgcggctggc gatgacgagt tttttcgggt tcatgttcaa tgcgttcaaa
    20041 gaatggaaac ggcgtgaagt ctatcatatt ttgaacgttt cttcagacgg caccgtcagg
    20101 tctatgttat atttacaccc acaaaacacg aaacccgcca caatgaaacg caccaaaaaa
    20161 taccctttct taacgttgca gcggcaacgt ttccatttga actttgaaaa cgcctcttcc
    20221 gccgccggca tcccagccga acgcgatttc taccgctggg cgtggtctgc cttgaaaagc
    20281 ggataccgcc gcgccgacat cagcctgatt cttctggacg aagaagaagc ccgagcctac
    20341 aaccgcgact accgcggcaa agattacgcc accaatgtct tgagtttcgc gctcaacgaa
    20401 ggcgaaatcc tgccctgcca agtttcggaa aaactgtacg gcgatttgat tatctgcccg
    20461 caagtggttt tgaaagaagc cgccgaacaa ggcaaaacac ccgagcagca ttttgcccac
    20521 ctgaccatac acggtacttt acacctgatg ggctacgacc acatcgaaga cgacgaagcc
    20581 gaaataatgg aagccgaaga aatccgcctg atgcgggcgg caggcttccc caacccctac
    20641 caagaggacg gacattaaaa tggacggcgc acaaccgaaa acgaattttt ttgaacgcct
    20701 gattgcccga ctcgcccgcg aacccgattc cgccgaagac gtattaaacc tgcttcggca
    20761 ggcgcacgag caggaagtat ttgatgcgga tacgctttta aggttggaaa aagtcctcga
    20821 tttttccgat ttggaagtgc gcgacgcgat gattacgcgc agccgtatga acgttttaaa
    20881 agaaaacgac agcatcgagc gcatcaccgc ctacgttatc gataccgccc attcgcgctt
    20941 ccccgtcatc ggcgaagaca aagacgaagt tttgggcatt ttgcacgcca aagacctgct
    21001 caaatatatg tttaaccccg agcagttcca cctcaaatcc attctccgcc ccgccgtctt
    21061 cgtccccgaa ggcaaatcgc tgaccgccct tttaaaagag ttccgcgaac agcgcaacca
    21121 tatggcgatt gtcatcgacg aatacggcgg cacatccggc ttggtcacct ttgaagacat
    21181 catcgagcaa atcgtcggcg aaatcgaaga cgagtttgac gaagacgata gcgccgacaa
    21241 tatccatgcc gtttcttccg aacgctggcg catccatgca gctaccgaaa tcgaagacat
    21301 caacaccttc ttcggcacgg aatacagcag cgaagaagcc gacaccattg gcggcctggt
    21361 cattcaagag ttagggcatc tgcccgtacg cggcgaaaaa gtcattatcg gcagcctgca
    21421 atttaccgtc gcacgcgccg acaaccgccg cctgcatacg ctgatggcga cccgcgtgaa
    21481 gtaagcagag cctgcccgca ccgccgtttc tgcacagttt aggatgacgg tacggacgtt
    21541 ttctgtttca atccgcccca tccgccaaac ataaaaatgc cgtctgaaac cggaaaacag
    21601 gttcagacgg catttcgctt atccgcttat tttttgctca aagtcagccc cgtccagccg
    21661 aataacaggg ttaaagccaa actcaaatag cagaaaaagg catagggcag atattcccag
    21721 accggcacac ccagcgcatg gctgataaat acgccgcaca cgctccacgg aacaagcggg
    21781 ttgatgaccg tacccgcgtc ttcgagcgta cgcgagaggt tgcgggaatg tagtccgagc
    21841 ttatcgtaaa cgggtttgaa cgtttcaccc gacaacaaaa tactcaaata ttgctcgcca
    21901 atcaggaaat taaccccgac cgaagtcata gcaacgctga atgtggcacg cccggcattc
    21961 gtcaaaaagc tgcggacggc atccagcagg gaaggaatcg cgcccagtgc aaacaacagc
    22021 ccgcccaaac tcatcccgag gataacgata gtctgcgtga agaacatact ttccaaaccg
    22081 ccgcgcgaaa tgagtttggc aatgtcttta aacgcttcgc cttcgagttt gtagccgccg
    22141 taaaaccacg cgccgagctg acgcagatcg ggcgtactgt gcagatacgt tacggcaacg
    22201 gcaaccatga cggtaaagag catggcgacc acggcattga cgcgcatcaa tgccaaaacg
    22261 accaacagtg caaacggaat cagcgaatag ccgtgcacca atcccgtggc ttcaagctgg
    22321 ctgcggaagg attcgacgct gttcaaatcc tgcgcggcga cactgggaag aagccacagc
    22381 atcagtgccg cactgatgag ccacgcggga atggtggtgt acatcatatt tttgatgtgc
    22441 tcaaacaggt cgatgccgac gatggacgcg gaaatgcccg tggtgtcgga aagcggggac
    22501 attttgtcgc cgaaaaacgc accggaaaca atcgcgcccg ccgtcatcgc catatcggca
    22561 tgaaacgccg ccgccatccc cataaaggca acgccgacgg tggcgcaggt ggtcaggctg
    22621 ctgccgatgg acacgccgat gacggaacac agcgcgaagg cggaaaaata aaaataagtc
    22681 ggggaaatca gcccgaaacc gtaatacatc agcgtcggaa tcgcgccgct catcatcagc
    22741 gcgctgacca tcagcccgat gaagaaaaac aggtaaatcg cgcccatacc ctgattcaac
    22801 gcgcctatca tgccctgctg catatcgttg tatttcaaac cgcgcaccaa gccgtacaaa
    22861 atcagcacga cgatggcggc aataatggac atatgcggca gccactccaa tgaaatgatg
    22921 gtatagccca tcgcggcaat cagagcgacg acgacggcaa gtgcctcacc gcgcggcata
    22981 tcgagtaagg atttgaaagc aaacatgatt ctttgcccga tttgatataa agttaacgga
    23041 ttctaacaca ccataccgaa tccgcccaac gttttcagac ggcattttag cgttttacaa
    23101 aagccggaca gaaacaaaac ggcaattttc tcaatttaaa ttaatttttt tgaaatataa
    23161 ttttttattt ttttgtttgt tggcatataa gacttttatg gattgaagta ttaaaacctc
    23221 ggcacacatg ccgcaaacac cggtacgcaa aaatgccgtc tgaaagatgt tcagacggca
    23281 tttctcaaga aaaagattat ttgtccgccc tatatccgta caccgcaaaa aacacgatat
    23341 acagataaca gatgacggaa acgacaaacg aagacatcag ggtgtaagta tctgccaccc
    23401 agccctgcac gacaggaacg accgcaccgc cgacaatcgc ggtacacagt acaccggaag
    23461 ccgcgttggt aaattttccc aatcctttgg ttgccaaaga gaaaatcgtc gggaacataa
    23521 tcgagttgaa aaaaccgatg gcaagcagcg accacatcgc cacatccgca ttgccgctac
    23581 ccgtcgccat cgcgacggca agcagtacga ccgcagccga tgcgttaaac gccaaataac
    23641 ggttgggcgc gaatttcgcc atcaccgccg aaccgaggaa acgtccgacc atcgcgccgc
    23701 cccaatagaa cgacaggtaa tgcgcggcag aagcatgatc cagccctttc agataaccca
    23761 atacgttgac catcaacgaa ccgatagaca cctccgcgcc gacatagcag aaaatgcctg
    23821 ccgtaccgaa cacgagatgt ttgtattgcc atacgctggt tttgccgtcg tggttgtgcg
    23881 cgctttcctc ggcggcaatt ttgcgcgcgt cgggcagccg gatcattttc acgaaaacgg
    23941 caaggataat cagcagcccc gccagtccca aatagggaat ctgtacggaa gaaatctgtt
    24001 ccgccttgct gacggtttgg gttgcgtccg ccagaatcag gaacgcgccg atttgcggcg
    24061 caatggtcgt acccaaagcg ttaaacgcct gaaccagcgt cagtgttgcc gattccttgc
    24121 cgggtttcgc cagcagggta acataaggat taccggcgac ctgaagcagc gttacgccgg
    24181 aagccaaaat aaacaacgcg cccaaaaata ccgcgtaaga atggctaccc gcagcaggat
    24241 aaaacagcag gcatccgacc gccgtcagca gaaagccgcc gataacgccg tttttgtagc
    24301 cgactttgcc gacaaaagcc cccatcggga tggacatcac cgcataggcg gtaaagaaac
    24361 agaattggat cagcatcgcc tgaacgtaag acaggtcgaa aatttctttc aaatgaggga
    24421 taaggatgtc gttcatgcag gtaataaaac ccatcatgaa gaacagcgtg gtcaagacga
    24481 ccaatgggga cgtatgattg ttttgtgatt gcgcagacat gatttgcctt tcgtggtgtc
    24541 ggaatattac ccgtttcaag aaaatagaaa ttgacatttc ttaacggcaa tgcaaatcat
    24601 actgatattt gaacattttg caaactttat cggatgcagg caatgcgccg atctgaaatt
    24661 caagcagcag aaattaatat tttacagttg cttgcataga ttttaataga ttttaataga
    24721 ttttaaaaaa ttccggcgga cttgcaaaaa ccgttcccaa taaaatgaaa atccgccgcc
    24781 gtatccggaa aattcggaaa tgccgcatcg tggcatcaaa accggagcgg aaagaatgtc
    24841 gtatgcccga caaaaaaggg gcatacggct gtgccgtatt ttttgcaccg tcctccgccc
    24901 ttcggcgggg gcgtgaaaat atagtggatt aacaaaaatc aggacaaggc gacggagccg
    24961 cagacagtac aaatagtacg gaaccgattc actcggtgct tcagcacctt agagaatcgt
    25021 tctctttgag ctaaggcgag gcaacgccgt actggttttt gttaatccac tataccgtac
    25081 aatccgtcgc cgatttttgc ctgtttgaga ctgtttgcaa tgatgcccgc ctacgcctac
    25141 gccgcgctcg ctttttccgc cttcacttcc gccaccctcc tgcccggcac atccgaagcc
    25201 gcctttgccc tgttcgtcca ccgctttccc gaagacgcat acggcgcgtt gctgtgcgcc
    25261 ggccttgcca acggattggg cagtatggtt tcctattgga tggggcgttt gctgccctcc
    25321 cgaaaaatgc cgtctgaaaa aacactgaat ctgatgcggc gtttcggtat ttggctgctt
    25381 gcgtttgcct ggctgcccgt cgtcggcgac gcactgccgc ttgccgccgg ctggctgcgg
    25441 ttgaatccgt ggacaagcgg gctgatgctg gttatcggca aaacggcgcg ttacgccttc
    25501 attctgtggg gaatgcaata ttacgccgcc taaaccatac gttataatgc cgaacacaca
    25561 aacccaccgt ataaaaaaat gaacagacac atccgccaag aaatcttcga acgcttccgc
    25621 gccgccaacc cccatccgac cacagaactc aacttcaact cccctttcga gcttttaatc
    25681 gccgttttac tgtcggcaca ggcaaccgac gtcggcgtaa acaaagcgac ggcgaagctg
    25741 tttccggttg ccgatacgcc gcaggcgatg ctggatttgg gtttggacgg cgtgatggaa
    25801 tatacgaaaa ccatcgggtt gtataaaacc aagtccaagc acatcatgca gacctgccgc
    25861 atcctgctgg aaaaatacaa tggcgaagtg ccggaagacc gcgaggcttt ggaatcgttg
    25921 ccgggcgtgg ggcgcaaaac ggcaaacgtg gtattgaaca cagcgttcgg acaccccgtt
    25981 atggcggtcg atacgcatat tttccgcgtg tccaaccgaa ccaaaatcgc ccccgggaaa
    26041 gatgtgcgcg aagtcgaaga caaactgatg cgcttcattc ctaaagagtt cctgatggac
    26101 gcgcaccact ggctgatttt gcacggacgc tacacctgca aggcactcaa accgcaatgc
    26161 caaacctgca tcatcaacga tttgtgcgaa tatcccgcca aagcctgacc gccgccgccc
    26221 ttcaaaatga aacgcttgaa aattcggacg gacgttgtag aatttcaacc tatgccgtct
    26281 gaagccgaca aaatgcgctt cagacggcat taaagccggt accggcatcc gacaaaggac
    26341 aaaacgtgtt caaaaaatac caatacctcg ctttggcagc actgtgtgcc gcctcgctgg
    26401 caggctgcga ccaagccggc agctttttcg gtgcggacaa aaaagaagca tctttcgtag
    26461 aacgcatcga acacaccaaa gacgacggca gcgtcagtat gctgctgccc gactttgtcc
    26521 aactggttca aagcgaaggc ccggcagtcg tcaatattca ggcagccccc gccccgcgca
    26581 cccaaaacgg cagcggcaat gccgaaaccg attccgaccc gcttgccgac agcgacccgt
    26641 tctacgaatt tttcaaacgc ctcgtcccga acatgcccga aatcccccaa gaagaagcag
    26701 atgacggcgg attgaacttc ggttcgggct tcatcatcag caaagacggc tatattctga
    26761 ccaatacgca cgtcgttacc ggcatgggca gtatcaaagt cctgctcaac gacaagcgcg
    26821 aatataccgc caaactcatc ggttcggatg tccaatccga tgtcgccctt ctgaaaatcg
    26881 acgcaacgga agagctgccc gtcgtcaaaa tcggcaatcc caaagatttg aaaccgggcg
    26941 aatgggtcgc cgccatcggc gcgcccttcg gcttcgacaa cagcgtgacc gccggcatcg
    27001 tgtccgccaa aggcagaagc ctgcccaacg aaagctacac acccttcatc caaaccgacg
    27061 ttgccatcaa tccgggcaac tccggcggcc cgctgttcaa cttaaaagga caggtcgtcg
    27121 gcatcaactc gcaaatatac agccgcagcg gcggattcat gggcatttcc ttcgccatcc
    27181 cgattgacgt tgccatgaat gtcgccgaac agctgaaaaa caccggcaaa gtccaacgcg
    27241 gacaactggg cgtgattatt caagaagtat cctacggttt ggcacaatcg ttcggtttgg
    27301 acaaagccgg cggcgcactg attgccaaaa tcctgcccgg cagccccgca gaacgtgccg
    27361 gcctgcaggc gggcgacatc gtcctcagcc tcgacggcgg agaaatacgt tcttccggcg
    27421 accttcccgt tatggtcggc gccattacgc cgggaaaaga agtcagcctc ggcgtatggc
    27481 gcaaaggcga agaaatcaca atcaaagtca agctgggcaa cgccgccgag catatcggcg
    27541 catcatccaa aacagatgaa gccccctaca ccgaacagca atccggtacg ttctcggtcg
    27601 aatccgcagg cattaccctt cagacacata ccgacagcag cggcggacac ctcgtcgtcg
    27661 tacgggtttc cgacgcggca gaacgcgcag gcttgaggcg cggcgacgaa attcttgccg
    27721 tcgggcaagt ccccgtcaat gacgaagccg gtttccgcaa agctatggac aaggcaggca
    27781 aaaacgtccc cctgctgatc atgcgccgtg gcaacacgct gtttatcgca ttaaacctgc
    27841 aataacacac ataatttaac caaaatattt tttaaatgat aaaatgcccg tcaataccga
    27901 ttaacgggca ttttaaaaaa tcagttaatt gatttcaaat aagtattttc cggaacagaa
    27961 aatactttcg cccattttgc caacaaaatc cgaaccgttg cggacagaaa ccgccccatc
    28021 cggacgctta acacccccat aaccatgcag ctgatcgact attcacattc atttttctcg
    28081 gttgtgccac cctttttggc actggcactt gccatcatta cccgccgcgt actgctgtct
    28141 ttaggcatcg gtattctggt cggcgttgcc tttttggtcg gcggcaaccc cgtcgacggt
    28201 ctgacacacc tgaaagacat ggtcgtcggc ttggcttggt cagacggcga ttggtcgctg
    28261 ggcaaaccaa aaatcctggt tttcctgata cttttgggta tttttacttc cctgctgacc
    28321 tactccggca gcaatcaggc gtttgccgac tgggcaaaac ggcacattaa aaaccggcgc
    28381 ggcgcgaaaa tgctgaccgc ctgcctcgtg ttcgtaacct ttatcgacga ctatttccac
    28441 agcctcgccg tcggtgcgat tgcccgcccc gttaccgaca agtttaaagt ttcccgcgcc
    28501 aaactcgcct acatcctcga ctccactgcc gcgcctatgt gcgtgctgat gcctgtttca
    28561 agttggggcg cgtcgattat cgccacgctt gccggattgc tcgttaccta caaaatcacc
    28621 gaatacacgc cgatggggac gtttgtcgcc atgagcctga tgaactatta cgcgctgttt
    28681 gccctgatta tggtgttcgt cgtcgcatgg ttttccttcg acatcggctc gatggcgcgt
    28741 ttcgaacaag ccgcgttgaa cgaagcccac gatgaaactg ccgtttcaga cggcagccgg
    28801 ggcagggttt acgcattgat tattcccgtt ttggccttaa tcgcctcaac ggtttccgcc
    28861 atgatctaca ccggcgcgca ggcaagcgaa accttcagca ttttgggggc atttgaaaac
    28921 acggacgtaa acacttcgct ggtattcggc ggcacttgcg gcgtccttgc cgtcgttctc
    28981 tgcacgctcg gcacgattaa aaccgccgac tatcccaaag ccgtttggca gggtgcgaaa
    29041 tctatgttcg gcgcaatcgc cattttaatc ctcgcttggc tcatcagtac ggttgtcggc
    29101 gaaatgcaca ccggcgatta cctctccaca ctggttgcgg gcaacatcca tcccggcttc
    29161 ctgcccgtca tcctcttcct gctcgccagc gtgatggcgt ttgccacagg cacaagctgg
    29221 gggacgttcg gcattatgct gccgattgcc gccgccatgg cggtcaaagt cgatccctca
    29281 ctgattatcc cgtgtatgtc cgcagtaatg gcgggggcgg tatgcggcga ccactgttcg
    29341 cccatctccg acacgaccat cctgtcgtcc acaggcgcgc gctgcaacca catcgaccac
    29401 gttacctcgc aactgcctta cgccttaacc gttgccgccg ccgccgcatc gggctacctc
    29461 gcattgggtc tgacaaaatc cgcgctgttg ggctttggca cgacaggcat tgtattggcg
    29521 gtgctgattt ttctgttgaa agataaaaaa cgcgccaacg cctgacccct ttccctgttc
    29581 agacggcata tgccgtctga agctttttgg agcaaaccga aatgactgtt ccctgtattc
    29641 cgcgcggcct cgtcatggcc gacattgccg ccttccgcct gaccgaagag gaaaagcaac
    29701 gccttctcga tcccgccgtc ggcggagtca tcctgttccg ccgcaacttc caaaatatcg
    29761 cacagctcaa agaactcacc gccgaaatca aagccctgcg cacacccgaa ctcattatcg
    29821 ccgtcgatca cgaaggcggc agggtgcaac gtttcatcga aggcttcacc cgcctgcccg
    29881 caatgagtac gctcggcgag atttgggaca aagacggcgc gtccgccgcc gaaaccgccg
    29941 ccggacaagt cggccgggtt ttggcaaccg agctttccgc ctgcggcatc gatttgtcct
    30001 tcacgcccgt cttagatttg gactggggaa actgccccgt catcggcaac cgcagtttcc
    30061 accgcaatcc cgaagcggtc gcccgccttg ccctcgccct tcaaaaaggt ttggaaaaag
    30121 gcggtatgaa atcctgcggt aaacattttc ccggacacgg atttgtcgaa ggagacagcc
    30181 atttggtgct acccgaagac gggcgcagcc tgtccgaact cgaagccgcc gacctcgccc
    30241 ccttccgcat tatgagccgc gaaggtatgg cagcggttat gcccgcccac gtcgtttatc
    30301 cgcaagtgga cacaaagccc gcagggtttt ccgaaatctg gctcaaacaa attttgcgcc
    30361 gcgacatcgg gtttaaaggc gtaattttct cggatgattt gactatggag ggcgcgtgcg
    30421 gggcaggcgg catcaaagaa cgcgcccgca tttcctttga ggcaggttgc gacatcgtcc
    30481 tcgtctgcaa ccgtcccgac cttgtggacg aactgcgcga agatttccgc attcccgaca
    30541 atcccgcttt ggcgcaacgt tggcaatata tggccaacac gctcggcagt gccgccgcgc
    30601 aagccgtgat gcaaacaacg gatttccaag cggcgcaagc ctttgttgcc ggccttgcct
    30661 cgccgcagga cacggcgggc ggcgtgaaag tcggagaggc cttttaaatc cgcccgacac
    30721 gcggcaaaaa ggcaaaacgg tataggcacg ccgtattttt tgcgctataa tcacgcctta
    30781 cttaattcgg aatgtagcgc agcccggtag cgcacttcgt tcgggacgaa ggggtcggag
    30841 gttcgaatcc tctcattccg accatattca aaccgcctcg gcatggattg ccgggacggt
    30901 ttttcctttt ggcggcaaca gaaaaatgcc gtctgaaaca gaatgctgtt tcagacggca
    30961 tttcgttttc ccccaaccct gccatttgcg cgcactggca cagagtatcg gcaaggttca
    31021 aaagaccatc ggaatttagg aagccgggct tgtacccaac gacggcatct tattcagtaa
    31081 ggctccgccc ccaatcaggc agcggaaatg ccggcagttt cacgcggaag cggcacactt
    31141 tccccgtatg tcaaacatcg gcaaaacggt aagccggccc tttgagttac actgcttaaa
    31201 ctactctatc cccgcccatt tgtgcgtctg cacgctcaac tgccaatgca ccggcgcgtc
    31261 ggggcggctg ttcagaatac cgatttggcg gatggtgtcg tagatgttca tcgcaccgtc
    31321 ttgctcacag ggcgaaaggt agtaatgatg tgcgcggatt ttgcgttcca tgttttcgca
    31381 gaacgcaagg acatcaccat cggcaacaat ccgcacttcg tcggctgttt cgatacagct
    31441 attttcatat ttggcggcat agcaggcttt ggggctggtg gcgacgtagt cgatttgcgg
    31501 cggcgcggga ttgagtccgt tggtttcgag acagaggaaa tagccttccg ccttgagcgt
    31561 gtccagcagc atatcgagat gcggctgtat ggtcggttcg ccgccggtga tgatgatgtt
    31621 gcgggcggca taggttttca gacagcctaa gatgtcggac aggctcatca tgccgaatgt
    31681 caaataatcg gtatcacacc agccgcaggc gaggttgcat ttgcccaagc ggacgaaaac
    31741 ggcgggcatg cccgtgttcc agccttcgcc ttgcaggctt tcaaagattt cgacgatgcg
    31801 gtattgggga ttttcggggg caacattgat ttttttcata ccgccaacac cgccgccgct
    31861 gccaaaaatg cgggcaaacc ttgtttgacg agtatgcctt tgttgccgga agagaacgcg
    31921 ccccacgcgg cggcaatcag gacgaagccg agaaacagta ccgttgcgcc gtacaccgcg
    31981 ttatccggcg cggcaaaccg cgaccacacc agcccgatgc cgagaaagcc gttatacagc
    32041 ccttggttgc cgaacagggt ctgcacgcgc ttttgttcca taaattcata aggcagcttg
    32101 aatatttccg ccgctttttc gctgggaatc tgcgtcattt caagccaggc gatgtagaaa
    32161 tgttcgacgg cgacgaagag gactaggagg atgaagagga gtttcatggg tattcctttt
    32221 aatgggtgta tttgatatgg aaggtcgtct gaaaaaacgg ggaatccgta caggagaaaa
    32281 aaccgtttta cggtaaattc aaataaggac gctctaaacc gtcctttccg cgcaggcggg
    32341 aatccagaga gttggaattg ctgcaatttt taaatattcc tgaaaaacca aggtctagat
    32401 tcccgcctgc gcgggaatga cggcttaggg caagttttat tcaaattcac tatatcgata
    32461 cgttcaaact atcccctacc ccccttcata ctccgcacac gatgtcggcg tttcccacaa
    32521 tttgacgcgg cacacgttca gccccgcgtt tttcagacgg tcgtacattt cgaccgccat
    32581 attttcggca gtggtgcggc agggcaggcg cagggttttc atgttccagc cttccaaaag
    32641 tgcggcgatt tgagattcgc gcccgttgcc gccgtggtag atgaaggcgt ggtcgaaggg
    32701 gtcggtaatg tgttgtttga caatggcttt caagtcggta aagtccatca ccataccgtc
    32761 tttcggcccg cctctgacgg ggtcgtctga aacggtgatt tcgagtttgt aggtatgccc
    32821 gtgcaggttt tggcatttgc cgtcatgccc gtcgagcata tgcgaggagt cgaaggtgaa
    32881 gattttggtg attttcatgg gttctcaaat gggcaaatag ccttgaaagc cgatttcggc
    32941 gggtttgccg tgcggattcg gggtcaggcg gatatggaaa ttgccgatgc ggtcgagcgc
    33001 aagcaggttg tccacgtctt ttaagttgtc tttatgttgc tgaacatagc tgtcggtcaa
    33061 cagggtttgc agggcttttt gtttggcttc ggcaggcgtg gcggcggcga acagcccaaa
    33121 agcgtgcgct tcggcgagct gccctgcgcg ataaccgccg acattggcaa aatacaggcg
    33181 cggggcgtgt tcagacggca ttttcgtttt gggcgtttcg gatacggcga tgtcgtaacc
    33241 gtccgcccat tcgacaatct gccagccgtc gatgtggatt ttgtccgcat cgccgaacca
    33301 cgcggctttg agtgcgggga cggcttcacg gtagtcgtcg cacacggcaa attggatgtc
    33361 gtgcacttcg atattcgacc tgccggcatt gccgccgagg taaaacatat ggagcttggg
    33421 catatcgtat tccttatggt ttgcgcgtgt tcagacggcc tttttacttt ccagatattc
    33481 cgccagcccg cgttcgcgca agatacagct cgggcattcg cggcagccgc cgccgacgat
    33541 gccgttatag caggtgtggg tttgctcgcg gatatagtcc agcgcgccca tttcgtccgc
    33601 caacgcccaa gtttgcgcct tggtcagata catcagcggc gtgtggattt gaaaatcata
    33661 gtccatcgcc aaattaaggg taacgttcat cgatttgaca aacacgtcgc ggcagtcggg
    33721 atagccggag aagtcggttt cgcacacgcc cgcgatgatg tgccgtatcc cctgcccttt
    33781 ggcgtaaatc gcggcataga gcaggaaaag cgcgttgcgg ccgtctacaa aggtattcgg
    33841 aacgccgttt tcggcagttt cgatggcggc ggtatcgtcc atcagggcat tgtgcgtaat
    33901 ctgccgcatc aggctcaagt cgagtacggt ttgtttgatg cccaaatcct gcgcaatcca
    33961 gcgggcgcgt tccagctcga cggcatggcg ttgcccgtat tggaaagtga tggcttggac
    34021 gttttcgcgc ccgtaggttt ggattgcctg aatcaggcag gtggtcgaat cctgaccgcc
    34081 cgaaaagatg accaaggctt gttggtttga catatcgaat cctagttttg gtaacgcggg
    34141 aggtttgcga accgcgaagt tgaacagtaa agatgcggat tatacaggca aacgcgttca
    34201 gacggcatct gaacgccatg ccgtctgaac acgcggctta tgccgacttg acggcgagtt
    34261 ccgccaagcg tttgctttgt gcgaaggcga tattgttttc ttcggcggtc aggacgggag
    34321 ccgctgtcgg gaatatcttt tttgacggct tcgcagacgg cggagacttt ggggacggtg
    34381 cgcaatacgg cttcgcaacc ttcaacgctg tcgatgccgc gagcgattcg gcgggcggga
    34441 ttgcgggggc tgccgttttg ggaatagtag aggacgagga ttttgagggg gatttgggtt
    34501 catgggcgga ttccgttaaa atggttcggt cttcaaacgg cataacagtt gttccgatgc
    34561 cgtctgaaac agccgcagtt caggcatttg ttcctgacgg caaaattggc tataacatcg
    34621 ggcatcaatg cccaacttac tgaccgggca agaaattatg acctttttac aacgtttgca
    34681 aggtttggca gacaataaaa tctgtgcgtt tgcatggttc gtcgtccgcc gctttgatga
    34741 agaacgcgta ccgcaggcgg cggcaagcat gacgtttacg acgctgctgg cactcgtccc
    34801 cgtgctgacc gtgatggtgg cggtcgcttc gattttcccc gtgttcgacc gctggtcgga
    34861 ttcgttcgtc tccttcgtca accaaaccat tgtgccgcag ggcgcggaca tggtgttcga
    34921 ctatatcaat gcgttccgcg agcaggcgaa ccggctgacg gcaatcggca gcgtgatgct
    34981 ggtcgttacc tcgctgatgc tgattcggac gatagacaat acgttcaacc gcatctggcg
    35041 ggtcaattcc cagcgtccgt ggatgatgca gtttctcgtc tattgggctt tactgacgtt
    35101 cgggccgctg tctttgggcg tgggcatttc ctttatggtc ggctcggtac aggatgccgc
    35161 gcttgcctca ggtgcgccgc agtggtcggg cgcgttgcga acggcggcga cgctgacctt
    35221 catgacgctt ttgctgtggg ggctgtaccg cttcgtgcca aaccgcttcg ttcccgcgcg
    35281 gcaggcgttt gtcggggctt tggcaacagc gttctgtctg gaaaccgcac gttccctctt
    35341 cacttggtat atgggcaact tcgacggcta ccgctcgatt tacggcgcgt ttgccgccgt
    35401 gccgtttttt ctgttgtggc tgaacctgtt gtggacgctg gtcttgggcg gcgcggtgct
    35461 gacttcttca ctctcctact ggcagggaga agcgttccgc aggggcttcg actcgcgcgg
    35521 acggtttgac gacgtgttga aaatcctgct gcttctggat gcggcgcaaa aagaaggcaa
    35581 agccttgcct gttcaggagt tcagacggca tatcaatatg ggctacgacg agttgggcga
    35641 gcttttggaa aagctggcgc ggcacggcta catctattcc ggcagacagg gttgggtgtt
    35701 gaaaacgcgg gcggattcga ttgagttgaa cgaactcttc aagctcttcg tttaccgtcc
    35761 gttgcctgtg gaaagggatc atgtgaacca agctgtcgat gcagtaatga tgccgtgttt
    35821 gcagactttg aacatgacgc tggcagagtt tgacgctcag gcgaaaaaac agcagcaatc
    35881 ttgagtattt ttgaaactgt atttttatcc taaagacatt ttcctatctg ctgattccac
    35941 cgtttccttt ctgtgtgccg ccataatttt tatgatagac aagccattca agtttgcatt
    36001 gactgctttt tcattggcaa tatccaaatg gctggtcgct gcatctaaaa ataggatttt
    36061 cggttcgcaa tattaaggcc cgcgccaata cgatgcgttg tttttgtccg cctgacagtg
    36121 cgctgcccat atcgccgatc aaggtttcat agcccatagg catggcgcag atttcttcgt
    36181 gaatcatagc aaggcaggcg caatgttcga tttttcctct atccggttcc gtatcgaaaa
    36241 aacaaggggc tgtactagat tagccctaaa tcccacacca atcccgcagg attttaagct
    36301 gttgagacgg tgtgccgaag ttaaatcgaa attcgcattc tttcaagaac agcgggaaag
    36361 atttacgatc gattccgttg tattttcgca agacgcgttt agtctagagt ctgtatatta
    36421 catttatttt tagggtctgc tagccaattt cttgttccct tcattatttt atcttctgaa
    36481 agaaaattat ttttttccat gctattaata ttaatgatat gatttttatt taaaataatt
    36541 tttccatata aaatttccgg tttgtaatag caagagtctg aagcaaatcc ttgcccgtca
    36601 atataagaaa aataaatgtt atcatcctcc ttataatatt ctaaaaccaa tccggatatt
    36661 attttatcaa aattcccttc atttccatct ttcatgacta aaatataatt agcaggccaa
    36721 cctttatcgt aaacatattc aaactgatag ttcccgaaac tctcgatatc cgaactaaaa
    36781 aagaagaaaa gcaaattaat agccaataca aaaacaaata agaacaaaaa tagcaaaatt
    36841 ttcaacttag ttaacaatat ttacctctcc tttaaattca atcctgaaag gtacccctta
    36901 ccctgggcta ccaattatag tttccatatt tctaaatatt gtttttacat tacttttttc
    36961 tccccccaaa ggaatgcatt ttaaaatcat gcttttcagg tgctaatcga tacttaccat
    37021 taacatcttt aatcacagat atatttccat gtatagccca acgtgaaaaa tctgagtatt
    37081 atatacagtt ataccttact gaaacaaagc tagctgtaca tatctgtacc atatttttac
    37141 tacaaacaaa aaatatttat aattattaag gataagataa aaaaccccaa cgccagatta
    37201 attcttaata atttcaaata aaaaatattt gagatttttt cttttccaaa taatatattg
    37261 aaatatttaa agttaaggta gcttgattta agtagaaaat caaaaaaacc attaattaat
    37321 ttttcttctt taaatatttt atatgatatt ataaaattaa ataacattgc gaaaacatat
    37381 gaaatcatca aaataccaaa tatatagtgg gaatgcatta ttatttagct ccttcttttg
    37441 gctttgacaa attgacagtc cattgatatc caatgcttgc aattgctgac gcacctgccc
    37501 ctgttttccc tgttagtatc aatgattctt ttcttgcaag atgtgcacct gcacaaccac
    37561 ctaatactaa accaccacct attgtaaatc cttcctattg tatctctaat gataacattt
    37621 tcttcttgtg ccgcccttaa tacagattgt ggaattggat tgacctgagc aggaacttct
    37681 aaaataagtt ttccttcaag ctgtcttcct cttagcagat taggtgtact tggaacattg
    37741 gaaatgacaa agtgagtgcg caaagccatc tgaaggcgat gtgtttgaac ctgttgaaag
    37801 cggccaacag gctaaatgcg cccgctgccg cctaaaaggc agcccggatg cctgattatc
    37861 gggtgtccga ggaggattaa gggggtattt gggtaaaatt aggaggtatt tggggcgaaa
    37921 acagccgaaa tcctgtgttt gggtttcgga tgtcggggaa gggctttttt gcaaaggtct
    37981 caactcatgt tattgcaaag atctcatgcc ttgttttgcc agcaggttga gttcttctcc
    38041 ggcaatcagc gtggtaaagc cccgccacaa cacagatcta tgtactatgg aagggcttca
    38101 atttatagct ttcgaaaatc tttcagcttt aagacggcct aaagttgtaa tctataaaac
    38161 aggagctcag agttttacgg ctcactctcg cttcgcgtta ccaaatacca gcttatcggt
    38221 tttgaacgta tagtaatcta tccaatcctg cagatattca tacgggtaag acaagtcata
    38281 ctgtttcata aaatcgaaga tgtcttgata ttcgtctgcc ggcagcggat cgtaaacagt
    38341 caaatccctc ggcgcgattt cgtggtctat gcccaaatcg aaaccgtgca tggatgcgat
    38401 tttggcgtag gtaacgattt gcggctgcag gtagaaatcg aaataggaca atgtttcttt
    38461 ggcagccata cgcgcggttt ttttatcaaa aagcggctct aaggcggcct tcatcgcctc
    38521 ggcatcctgt tcggcgaaag cgaggaagaa gcggtaatcg tacaaccgct tttgcagcca
    38581 tttgctaggg gtgggatgcg ccaagacttt ctcgctacgt tgtttcaacc gatcaagctg
    38641 cttaccctcc accatcagca gagtattgta aatcatatga cggttgaggt cgtatcggtt
    38701 tacgaaggct tctgtatcgt tggcgatgtt gtcgatattg cgcaccaaaa actcgcgcag
    38761 ctgcgggctg tcgctcatca gcatcagaaa catcggatct tgaatgttga gcatgtcgca
    38821 gggaaagaag aagggttcgg ggtcttcatc atctacactc tgcaaaatac ccaactttcc
    38881 tgcaacataa gcatattgct tgaatttctt aagatcatgt tcaaatagat aggcatggga
    38941 agccgcagct tcagtgtaca ttaaaatgta tttcatcgct gcaagcggca cgcctttttt
    39001 ttcatctaca taatctatag attcggggca accgctattg aaattagcag tattgcctat
    39061 gattacatta gtaatatgtt cataccattt ttgggtggtc atcatattgt gccccattgt
    39121 tatctcctta tattggtttt agaaggaact ttgacagaaa gaataacggc cttacctgtt
    39181 tgacgatcaa cgcctgctat tgccgttttt aatttgccgt tctgatttgc ttttaagaca
    39241 actgccttag caggactacc atcaggtaaa ctttttacaa cttgtttaat ccattcacga
    39301 ctcatctgcg tatatccacc cgcaccattc ggattcagct gtaccgtacc gttcctaatc
    39361 tgcttacttt ctacaatcaa aacaacacta ccatcggcag cctgccatac atgatcaaaa
    39421 ccgttatttc cgccgtattt gccgcccgaa agcactctga agccattttg tttagctaaa
    39481 gagtcaaata actgctcggt cattttacct gtcgtgtccc ttccttcgcc aatagctttc
    39541 agcaattgtt tgtcagttgc tgcagtaacc cttaattcag tttgaccgat tttgattttg
    39601 gttttactga catagccctg tgcagtggca acggttgctg ccttgtccaa ggcttttttc
    39661 atgccttgta aattttttgc cgctttcgct actttatagg cctgtatcga ttcaccgata
    39721 cccggaacca cacccagcaa agcaaacagg tgatcggcag cggtctgtgc ttgtacaaaa
    39781 ctctgtatat caccgataat tggaataaat cctgtctgaa gaagcaaaac ttctcgatat
    39841 tcttcaggat tttcacgtat ccatttttgt tgttcctttt gaaattcatc acactttgca
    39901 ccaacgcatc taggatacaa agtattattc tccaccgcta tctgtgccgc attcgccgct
    39961 gtattcacat ccccgcctac aacggcagac gcagtgccgg caaccagttt tgaataggca
    40021 gtaatcttcg ctttagcttt ttcgatttcg gtcgcactca tacgactgaa atcagtattc
    40081 ttaaccaaag cctcaccaac aatctcaccg accgccgctc ctatcgcacc atcctgacac
    40141 ttgcccttat tcgccgccgc agccgcacag cccgctatgg catgggcaat cttgtggact
    40201 atgtagtgct gatccaactg tttgatttta ctggctgctt ctccatgcgc ggtattgacc
    40261 aaagccgcaa ggatattcgc ttccagattg tctttcaggc tgccgccgtt aacagtggta
    40321 ttaatcagcg cggcactgcc cgcattggcc aggttgacgg ttaggtcatt gacccactgc
    40381 ttatcgctga tattgttcag tgccgaagcg ccgattttgt cggctacgcc tgcggtagcg
    40441 gcggcaacca ccagattttt caccgtgcgg cttctgccca gctctttcag ggtgttaccg
    40501 atattgcctt tgttgttgat cagggaaatg gaagcctggc tcaccaatgc ggtaaaggca
    40561 gcatcggcca cggctgccga tgcggtaccc tgtgccgctc cgataaggcc tgcgcctact
    40621 cctccggtta ccgccgctac tgccagggcg ataattgcgg ctccggctcc ggttaggcct
    40681 tcctgtttat agtcccattt gtcgtaagcg agctgtactt ggttccagtt cacgtccttg
    40741 accgtctgaa gctgtttcag ataggcatat tcgggctgtt tggccagctt ttcgatttcg
    40801 gttttgaggt tgcctttggg gatgtcggcg atatagccgc cgggagcggt cagcttaggc
    40861 agtgccggcc cttcaaagct cggtagcttc agcgtttcaa ccgtgctgcc gcttccggcc
    40921 tgcttttgcc atacggtcga gttggattcc agcttttctt cggtttggat gcggttaacg
    40981 atgcctttta ggataatttt cgcatcggct cgggcttttt cacccacccc tgcctgtatg
    41041 tcggctccgg aaagggtggt tttgaattcg gtgccttcga gtacggtatc ccagccggaa
    41101 cgggttttgg ctgtttgggc gataacgcgt acgggcagtt tggtttcgtt cagctcgttt
    41161 ttgctgtaat tgcttttacc cactttgatg ccgatgaaac gggtactttt ctgaacattc
    41221 aattggtgtt ggtgaatgcc ttcggctgcc agcagttgca ttgaggccgt ctgaaaaacg
    41281 gatattcaga cggcctattg ctactttaaa taatatttgg gtttaaaata tcatcgtatt
    41341 tcccaaattc ggggaacaga aaatcaatta tttaaatttt tcatcctcat aatatgggtg
    41401 atcaaaaagc tctccttccc acatatttat ttcttctatg cctttatctt tcataaactc
    41461 atgataatca taaaaatctt ttccatattg tccgtaaaaa attttcatat ccccaagcca
    41521 catagcataa cattcatcgc ataaaatgac ctccccagag aaatatttag gctttgcatg
    41581 ataaaggtag ccttgctcac atcttgggca aattttaatt tctttatcca taagagattc
    41641 ctttcttaat tatttctgag gatatgcaga aatgatttta tttgttcctt tttgcacaac
    41701 aatccgcact tttgtttctc cttttgttcc aataggtttc ttcatatcta caagatatac
    41761 ggatgaatca tttggcaaag gatttccttt cattctccaa gcttcatcaa ccaaaggcaa
    41821 aacttcttta cgggacacat taaaaacaga atgaacaggt ttgtttggat ttggaattgt
    41881 atgggctaaa acatgtttaa tcctattacc atgcttagca tctaacccat aatctaaccc
    41941 cgccggtgtt gtccaaacct tattaacctt atcataagta agcttgattc cggtctgctt
    42001 ctgaatttcc tgtatggctt tagccgtatc aactgcttcg tctcccgcct tcagaacata
    42061 agccctgcct cggctgattt tagtcgcacc tgccggaaca aagggaacgg cagctgcggc
    42121 ggcatccaca ccaaaatcaa tcgccgcttc tcgagccatc tgcttatcgc caatagattt
    42181 ggcgtaaatc catttaccgc cgtcccatac gaggttgccg atatcccaaa taatatctaa
    42241 tgcattattt ttaacagctg tttcggaagc atttgccgcc acatcaacat tgcctcctgt
    42301 aacacctaca gtcgctccgg ctaccaattt tgcatacgca gtaatcttcg ctttagcttt
    42361 ttcgatttcg gtcgcactca tacgactgaa atcagtattc ttaaccaaag cctcaccaac
    42421 aatctcaccg actgcagcgc ctatcgcacc atcctgacac ttgcccttat tcgccgccgc
    42481 agccgcacag cccgctatgg catgggcaat cttgtggact atgtagtgct gatccaactg
    42541 tttgatttta ctggctgctt ctccatgcgc ggtattgacc aaagccgcaa ggatattcgc
    42601 ttccagattg tctttcaggc tgccgccgtt gacagcggta ttaatcagtg cggcactgcc
    42661 cgcattggcc aggttgacgg tcaggttgtt gatccactgc ttatcgctga cattgttcag
    42721 tgccgaagcg ccgattttgt cggctacgcc tgcggtagcg gcggcaacca ccagattttt
    42781 caccgtgctg cttctgccca gctctttcag ggttttgccg acatcgcctt tgttgttgat
    42841 gaacgatacg gaagcctggc tggccaaaga ggcaaatgct gcatcggttg cggcggcggc
    42901 cgcaccgttt aatcccaata cggctccggt tcctgcgcct gaggtgacca cggtaacggc
    42961 cagtgcgata atcgccgcac ctgcttcggt taagccctcc tgtttgtagt cccatttatc
    43021 gtaagcaagc tgcacctgat tccagttgat gtttttcgca acttgaagct gttttaaata
    43081 agcatactcg ggctgcttgg tgagggtttc gatttcggtt ttgaggttgc ctttggggat
    43141 gtcggcgata tagccgccgg gagcggacag tacgggcgca acgggaccgg tgaaactcgg
    43201 caattgcaag gtttcgatgt tactgccccg tcctgcctgt ttttgccata gagcagattt
    43261 gctgctgctc acggtttcgt tgtggatcgt ggttttgatg ccttcgagga taatcttggc
    43321 atctgcccgt gcctgctcac ctacgcctgc gcgtatggtt gcgccaccca gtgtggtttc
    43381 aaactgtgtg ccttgcagtt tggtatccca acctgattgc agattggctg attctgcaac
    43441 tacccttgag ggcagcgcgg ttttcatgac ttgggtggtg gtgtcgtgtg ctttgctgta
    43501 gctgatgcca agaaatctgc gccttttttg gctgtcaagt ttgtcgtagt tgagctcttc
    43561 tacggcatag agtgtcaatt tccgcccggc ttcgatgtta atgctgcctt tgggggcatc
    43621 gaatgcggtg gcgtaggcgt cgatgctgcc accagatttg atgtcgatgc cttgggatgc
    43681 gctgaggttt actgcgtcgg gcttggcgtt tttgtgttct ttgacttcgg taatgtgttt
    43741 gcggttgtac catttgccgg ttttgtagct gcggcgttca aaggtataga gttcgctctg
    43801 tctcgcatag taatattggt caccgtaaga ttgaattttg attttgccgt tttcagcttc
    43861 gatgtttgcc gcactcagac tgatgcggct gttctcattg gcatttggtg cagagagggt
    43921 aacacctgtt tttcctgaaa tttcagatgg cctgctgaaa atggcatagc cgtatttatc
    43981 gctgccttgc tgaccatatt caaaagtatc aagtacaccg ctgatgtcaa tggctgattg
    44041 aagtcgacct tcttgcagtt cctctgcggc tggtgcaggt aaaaatcctg cagcttggat
    44101 atttgcctgt tgggtggcag caactttgga gccctggata cggataccac ctgcagcgag
    44161 cagtttgata ttttgaccgc taagggtgct accttggttt tcgtgaccac tacccggttg
    44221 ggaaactgtt aaaagggctt gttgtaacaa ttgtttacgt tcaagtaacg cttttactgg
    44281 ggattggcgt tctgcccaag cctcatcagc agcatttatc tttgctcgta aaccacgtaa
    44341 tctggcctca ctatcaccga atactttgtc acgcctctta tatttacgcc tcaacatttg
    44401 tggaagatcc tgtgctttac gatatttagg atcgctcgtt aacttactaa gctcctgctc
    44461 tacatcagta agttgctgtg tgatattgtg tttatctgtt ttcaattgat agttactaaa
    44521 cgtattccta atactgttta gtgaaatatt ccctttagtt gccactaaag ccaaatcttt
    44581 ccctgcggca atatttgctg catcaatatc cacctctccc gccacaatcc cgatattgcc
    44641 tttagctgaa acgctggctg tttgcgcact cggattgccg ttatttcctg ctctggcact
    44701 gatttggaat gtgccgttgt gtttcacggc caaatcacct gcagaaacga tacggttacc
    44761 gggttcaacg gttaaagggt tatcaccgcc cgattctatc gacatcatac cggacagtgg
    44821 tttcagttcc gctgaacgca tagttgccgt atcttgggtt tcccaatcga tatgcccccg
    44881 tttgatgatg cctccgccag ctttgatgtt tatcgcgcca ccgcgcaggg tagtattgtc
    44941 caaaacctgt aatgcgctgc tgttcaatga caatacacca tctgccgcca aagtactttt
    45001 actgttttgc tggtcgtttt gtaaaatttg accgctgtct tttcctttgc ctttaccctg
    45061 tgcactgata ctcatatgac gacgtgaatg agcatcaagc tgattaagcg tcatatgacc
    45121 atgatttgta ttaatctggg tattgtgatc ggaattgagc ttgctgttat taatggttaa
    45181 agtttgatcc gcatccactt tcagtgatgc gcgggcattc aggtgtacat tctgcaccac
    45241 catgctgcct ttattgctgt ctaagctgat atgaccgccg ttaacggtgc cgttgcctaa
    45301 atctaattga cctccggcaa ccagtttcac atcgcctgca gaagcataaa tatcggtatt
    45361 gtccattttc agacggcctt taccgactga accagcatta atgtctcctt ccgcaatcaa
    45421 ggtatttaga ccgttgaaat caacattgcc gttggcaaga agagatacgc gtccaacttc
    45481 ggcaacagca gtcagaccgt ctgaaataat attgccttga agtgctgccg tatcaatatt
    45541 ctgactggca tttatccaac tctgattcaa gctgatgttg cctttagtcg cgctcatctg
    45601 cacattaccg ccactggatt gcagattact ttgacgcaca ctcaagctac ctgcatgaat
    45661 atacagattg ccggacgcat tcaggtggga ggcttttgcc tgaatgtcgt catcagccat
    45721 caataccacc tgctttccgc ctttaatgct accgttgttc aaacggatgt tagaagcaac
    45781 gtttgaagtc tctaaagaaa gcggtttacc cgcttcaatg tgtgcagtgt ctttagcctc
    45841 aattacagca gcactgccaa tactgccgtt ggacaatacg gttacgtttt cagcaataat
    45901 acgggtattt tcacccaatt cagtatcgcc tttggtggag ctgtacacgg aactgcccgc
    45961 ttgaatagta gcatcattga tcgtagtacg accgccggtc gacagattgg tcgaaccttt
    46021 agcattgttc acatttgctt tgctttcgat aaccaaatta cggccggcat ttaataccgt
    46081 agcagcaggg cggttgccgt tgttttgaac gaccgctccg ttacgcaaac cgatgtcctc
    46141 cccggtatca ataaccaata agcctttgct ctctatccgg ccgccattgg agataaatgc
    46201 gcctgctgcg cctgtttcgg tggtttcgat ggagagataa gtcggtgatg cttcagtgct
    46261 gtcggcagtg gtggcgatgc ggccgctgtt ttcaatgcgg cctgacgaag tcacaatcaa
    46321 ttgcttggcc gcttcgagtg tgccggcatt tttgacgcct acgccttttt cattggcaat
    46381 cagtgtgatg ctgtcggcgt acataccgcc cagtgcggca gtatcaaggg caatagtcgg
    46441 tttcgtaccc gctgccgtac ctgcactgat ttcgccgctg gcgtaatcta ctttctgagg
    46501 accggtagaa accgccaggt ttttaccctg taatttcccc tgcaaagcaa ctgcacgagc
    46561 aagtatctcg gtgtagtcgg ctccgccttt atcattccaa cctgctgctc ctacggtcaa
    46621 tgtgccttga cgcacatcaa atcctgtcag tgcaccgtct ttgccgattt ggggcgcacc
    46681 ggtagttaag atgccccgac cgacattttt aaagccgccg ccattaacgg taatgccgtt
    46741 ggggttggca ataatcacgt cggccttttg gccgcctacg gtaacgatgc cgttgagttt
    46801 gctagccgta ccgcgtacct cgttcaaaat caattgcgca ccgcctttga ccagaaacgg
    46861 attattgtta cggtcgttgt ttaacactgc ccctttgttg tcaacatcaa actgcgtata
    46921 gcggttgtgg ctcaatccgc gtccattcgg agtttggata ttcaccaagg gggcaccagt
    46981 gttggtttta aggataacga cctgctggtt tttaggtgct gatttgtcgg tggtaatttg
    47041 ggcatgggca ggcaatacca tactcaggga aaccaaagag cagaccaagg ttttaagggt
    47101 ggttttgagt ttactgtaaa ggccgtctga aatttgtggc gaagccggag cggaagttcc
    47161 tgcctgtttg cctttaccct gactgttggc ggtttcggca actgcaacca tagtgttgtg
    47221 ctttttgctg aaaacggtac gatgcagacc tttgttcata tttatccctt taaatgatgt
    47281 ttgtaatcgc ttcagacggc ctgtaaatga tgaaagactg tctgaatgtc ggtaaaaaat
    47341 gcaggagagg gttagaaact gtaattcaaa ttgaagccgt aaacggtgtt ggccgtctga
    47401 aaaccttcgg gcttgtgcag cggcttgccg gcaaataagt cataagcaaa tataccgcct
    47461 actttatggc tgcctctgaa gccggccact gcacccgcca gccgcttgcc cgatacatat
    47521 tgtgcacttt cgccagatac gcggccatag tccgcaccga gatagaactg atggttcgga
    47581 tgaaaatacc aagttaaagt attctgccag tagaaacctc gctctccgaa aagactctgc
    47641 tccccatcaa atccgcgaac ggtgtagcgg ctgccgattg acaatttatc ttgggcaacc
    47701 aacggcgttt tgttccattg agcttgaatg gcggttgcgt agaaaaactg ctgtttgccc
    47761 aacataaacg gggccgctgc atccaatccg gcggttatga ttttcatacg ggatgtgcct
    47821 ggaatagtac cgccgccgtt ttcttcaggt gcgggcatac tttggcgcat accggtcccg
    47881 cgtttgtaag acaacttgcc gtcaagctgc caacggttga ggtaagcacg gtggcgcaat
    47941 tcggcttccc agcctgcaga gcggcggcgt tgcacttcga tttcggcatc gtcgatgtat
    48001 ttataggttt ggcgtgtcca taatttcatt ccgactgaag ttttatgaag tctgttacgc
    48061 caaagcatgc gctcggcggc caggctgctc tgatattgtt tgccgttgta atcgtaattg
    48121 acggaatagc cttcggttgc ttcgtggtaa cgatgtccat tgtgattaaa agaaaacagc
    48181 cattttttta cgggcaccga ataatgcacg ctgtaacttc tggatccgct ttcagtttcc
    48241 gtaccggtgg catcagtcaa gtccgttttg tgcgccaaac cgcgtccata tgaaacataa
    48301 aacaaatcgc ttaagcccaa agggttatcg aacgataaag cgacatttcc ttgatatttg
    48361 ccggtcgttt tgccgcccgc atcatctata ccgatactga accgtatggg tttattctgc
    48421 tgccatttga tctgtaaatc gcttttgcct tcttcttcgg acggtataat ctgaatatct
    48481 gttttaacac tcggcaaacg acgcaggttt tccaagccct gctctacatc gcgaagattg
    48541 agaattttgt tcctatataa gggaaatttg ttattgaatg cactaatact gccctcggca
    48601 gacttcccat cccgtttttc ttcatagcgg atatccccta tttcgcctgc tgatacccgt
    48661 aatttcagaa ttcccgaatc catattctgt ggttggataa tagcttggga agtgaggtag
    48721 ccacgcacga tcagtatctg ttgcgcggct ttttgtagcc tgctcaaatt attggaacct
    48781 aaacacatcc cagttttaaa agctgtttct ttcatgagca cagaaggaag aaaagaaaat
    48841 ttgcgcgccg tcttatcatc taaactaatg taatttaccc gagtacacgg tgtttcatct
    48901 tcactcagga cataattgtt cttctccaat ggttgctcga aacggacatt tgcatcagtt
    48961 aacaattcag catctatgtg ctgctgacgc tgcatggaac ggataagttc tgcatcgttt
    49021 tcatcggcag ctaaggtttt aaggggtatg acagccagga taaccaacag acatggagca
    49081 ggaaaaaatt tcatgacatc aatattattt tagcaatatt tactattttg tcataaattt
    49141 aaaagtattt acagttatag aatgagacct ttgcaaaatt ccccaaaatc ccctaaattc
    49201 ccaccaagac atttagggga ttttggggaa ttttgcaaag gtctcggaca gtattttgaa
    49261 cgcagtgcgc gtaaatccgt atggaaacca tgaaatcccg ccacagccgc cagacatgcc
    49321 aagccgcatt ctgatatttc tgtttacagg ataacaggca gctttttctt taagcccaaa
    49381 gacaggtttt gcagatgggg catagatttc ctttttgaaa aatagggatt aggaagttgg
    49441 atgtatttta gaaaggccgc ctgaaaaggt ttcagacgac cttttgcgac tagctgctat
    49501 tttatttaaa gcttttctct aacaaacgag ctaatatttt tcctgtaata aaacagataa
    49561 aaaacagcat ccaatacgtc agattggaaa aatcggtcgt atagagaatc aacatataaa
    49621 gaagcagcat gatgccgagt gcgatgaatt gataatgttt ggcaaacatc atgacctcct
    49681 caactattaa ggcaaaccgc ctgaatattc tcgttcaatc gtttcggcaa tttccctata
    49741 acgtcgatac catgaccagt cgaaattttc aatggcatgg ctcgcaaacg taccaaattc
    49801 aggcatccct atgcggctac ctgctaaagc tccgattgta gctccccaac caggcggatt
    49861 actgaacgta ttgtttggca atcctaaaga tttatcagga tttcccgtat cgttaagccc
    49921 tgcagattcg caaatttcgc aataatatga gctttgttgc gcattacctg agcctccgac
    49981 ccaagtcatt tcatgaacac atatagtgga ttaaatttaa atcaggacaa ggcgacgaag
    50041 ccgcagacag tacaaatagt acggcaaggc gaggcaacgc tgtactggtt tttgttaatc
    50101 cactataaaa ctctcatttt gaaactcctt gtatcgttaa tcaaacaatc aaaagggcag
    50161 atgccctatc cttgctttta caaacggagt gcctgtaaaa ggggatggtt tcaggcagtt
    50221 ttgaagtttg tgttttttat tgtcttctgg tcgtctgaaa aggtttcaga caacttcttt
    50281 atctttcagc ctcaagtctt acagtttgcc cgacatacta taaatcagct ccaataccca
    50341 ttcgtacagc cgccgtttct cgtgcaggat gtctgcttcc aacgtcatgc cgatttgcag
    50401 cggtttttcc tcaccgtatg cagtgatggt tggtttgtcg ggttttattt tcacgagata
    50461 aacaggttcg ttgctcttcg ccaaatcgga ggataccatg cccaatcccg acaattcctg
    50521 tctgcccagt gccgtttttg ctactgatac gacactgccg gaagcaagcc cgaatttttg
    50581 atagggatat gcctgataac gtaggacaac cttgtctttc ggcttgataa agcctgctgc
    50641 actgctgggg atatatagat gggcatatag ctcggtacgt tcgggaacaa tgctcaagag
    50701 cagtttggaa ggatcaacct gctgtccgac ttcgacgttc ggtattgcta tataacccga
    50761 ccgtcctgca cggatgattt gttcagagcg catttcaaaa tccaaaactt cttgagaaat
    50821 atcggcaatg gtgcgttcaa gccagctttg ttctgtctca tgccgcttgg gggaggctgg
    50881 ccaatgtcag attctgcgtg cggatttcct gaagcagccc gacttcttct cggcggtagg
    50941 catcaagttt ggctttctgc tctaaaagct ctgccttgac attcatcatt tcttgttttg
    51001 gcactgcatc attggcggat aggaaacgat atttctgcaa catttcttcc gcaagtctaa
    51061 tgcgcctttt ctgaccgtct atctgttgcg aaatatggag ttcctggttt tccaaacgtt
    51121 cgacagttgc tttaaggctg cgcgtttcat tcccgtgtat cagcttcaga cgacccagtt
    51181 cctgttctgc caacgttttc ttcaaaactg cctccgtttt caactgctgc tgcacgctac
    51241 ctcctgcgcc gaaacgtgag gtcgaaagcg caaatagctt gtcgccagcc ttaacctttt
    51301 ctccatcttc cacgaatttc gctgtaattg tccccgtatc cggtgcatac accctgatta
    51361 cgcccgatgc aggtaaaatt tgtccctcca ctgttgtctt tcgcgtatag ttaccaaata
    51421 tcaaaaacag gataatcaat aacgcagata tcgatgcaaa tgtcgtccat agggaaaatg
    51481 acaacggtcg tgtcagaatc actttacccg tcaggctggt ttggcgggca acggcgactt
    51541 cgggacggaa gaagggttgc ttgggtctat tcataaaatt gaagttaaga aagtttcaga
    51601 cgacccctag agatcgtctg aaagatgaga aatatcagca gtaatctgta ccgtcagtgt
    51661 agccgtttcc tgatttatct gcttttgttg cgggagcagt taatccatgt tcaatctcaa
    51721 agattggtct tccgttataa ggaggtgcat taacggcatc atttacccaa tcacgagtca
    51781 gattgtatac accgacctcc tttggttgtg gcagcattag ctacatttcc agctatattg
    51841 tctgttacag gacctccccc tgaaaccagc ttcaattcat gaagttgaag ttcaatcatt
    51901 tttatactcc ttttcttggt tggtattcct aaaaattcgg ctaacaaaaa catatggcag
    51961 atatattgaa aaaaaattca aagtaccctg aataaaattc aaattccaac tatatttgtt
    52021 aatgtagtcg agaagaaaca tatctgataa aaaatatagc aattgataac aagctattac
    52081 taatattacg aaaaatgtaa attgcttcca gtttttcata gaatccctca caaaagttcc
    52141 agaaaatcta actctatcaa ctgataaatc aacttcctaa cttcttcatg ttttccctga
    52201 ttgaagttaa ccagtagatt tttcaacaat aacggttcat tcttaccgat atgttctaac
    52261 acttttttcc ccaactcatc tacgcttatc ttcatcccat tcccaatcaa atatcccttt
    52321 tccaacgtat ccaaattatt ggcatttagt ctcaacctga cgtcgtctga aagcggagta
    52381 gcgttgggat tcgcgaactg ttcgagatga aaagcggtat cggtacgttc tttgccgagg
    52441 aagtcttcac tgaaggcttc gtaattgatg gggtcggcaa tcatggcagc aatttgtgcg
    52501 gcagtatcgt tgatacgcgt cctatcttgc tcccagtctg agaaactgtg gcgcagactt
    52561 tctatggtgg ggaatttctt cattagccac tcgaggtaat tatagccgtt gggcggaaag
    52621 gtaccgacag cgaggtggaa ggtttcacag ccgagcggga taggtctgtg ccaccaaccg
    52681 cgcgggatgt agaggacatc acctgcttca aggataatat ccatatcgat atgttcagga
    52741 atggaaatat cagtatcttt agtctgttgc atatacaatg gcatagggaa atcaggggca
    52801 gtaagttgcc aacgtttctt gccgaaaagc tggatggcat acacatctcg gatgtcccaa
    52861 tggtttttat aagattcgtc gctgccaaaa gcaagatatc cactaacaat agtatgtgcg
    52921 ccggcaaagc gggcgacttg acgggcgata tggtctgaaa acggctcgtt gttaatatgg
    52981 ttatagacta acgacgcacc attcttcata tgttcgtaga taacggattt aataaaacgg
    53041 tagcgagttt tgcccaaatc gtcgaaactt tcgacgtatt cttctttagg aacgattgcg
    53101 ccttttttac gcagatgaaa cagcggtgcg gttgggtctg ctcgttggta tatctcgttg
    53161 atatctttcc aagatgcgga ttcgagattc cgaaccgctc ctttaaagag cttgggcttt
    53221 tgatacagat aagtctgtcg gaacgtttta ggactaatgc cgaagtcgag atggatgctc
    53281 attacttccc cttactcaga aaatatttaa aatttataat gttacatata tttacaaata
    53341 ttaaagtttt ttttgtgtgt gcgtcaagga attgttgcca attttgttat agtggattaa
    53401 caaaaatcag gaaaaggcga cgaaaccgca gacagtacaa atagtacggc aaggcgaggc
    53461 aacgctgtac tggtttaaat ttaatccact ataaaaattt gttttgtttc caaacgaaaa
    53521 aaagcggttt gttttttgtt gttaacattt ttattgtaat taaataaaag gtcgtctgaa
    53581 aacggttttc agacgacctt ttgctataat cgggcttcat cgccccgttc ggtttggaac
    53641 cttatgaaaa ccctcgtcct cctcctgctt tccttctcca cgaccaccgc tttcgccgca
    53701 tacggtttgg gtttggggca ggcaccgaaa tatcctgccg actttcgcgc ctacggttat
    53761 gtttattccg gacggcaggg ctgggtttta aaaacagagg cggatgccat taaattagac
    53821 acgcttttca aacgctttgt gtaccgtcct tccgccgcca atcaaaaccc cgtcggacaa
    53881 gccgtcggac agcgttcgga cggcataccc gccaaccaca caaaggaaaa accatgagta
    53941 aaaaaatcaa agtcggcatt gtcggcgcga cgggctacac gggcgtggaa ctgctgcgcc
    54001 tgcttgccgc ccatcccgat gtcgaagtcg ccgccgtaac cagccgcagc gaagcgggaa
    54061 ctgcagttgc cgattacttt ccgagtttgc gcggcgtgta cggcctcgcc ttccaaacgc
    54121 ccgacgaggc aggtttggaa caatgcgaca tcgtcttctt cgccacgccc aacggcatcg
    54181 ccatgaaaga cgcgccgcgc ctgattgaac agggcgtgcg cgtcatcgac ctttccgccg
    54241 acttccgcat acgggacatt ccgacctggg aacactggta cggcatgacc cacgccgccc
    54301 ccgacctcgt ttcccaagcc gtgtacggat tgagcgaact caaccgcgaa gccgtcgcac
    54361 aggcacgcct cgtcgccaac cccggctgct acccgacctg cgtatccctg ccgctcgtgc
    54421 cgctgttgcg gcaatgccgt ctgaagcccg gtatgccgct gattgccgac tgcaaatccg
    54481 gcgtgtccgg cgcgggcagg aaaggcaatg tcggttcgct gttgtgcgaa gccggcgaca
    54541 acttcaaagc ctacggcata gccggacacc gccacctgcc cgaaatcagg cagaccatcg
    54601 ccgggcttca ggacggcatc gccgaaggat tcgtgttcac gccgcacctc gcgccgatga
    54661 tacgcggtat gcacgccacc gtttacctcc acctttcaga cggcagcaac cccgaaaccg
    54721 ttttgcgcga ctattatcgc gacagcctgt tcgtggacat cctgcccgcc ggttcgacac
    54781 ccgaaacccg cagcgtgcgc ggcgcaaacc tctgccgcat cagcatccaa caggcggcgc
    54841 aatccgatgt gtgggtcgtc ctttccgtca tcgacaacct cgtcaaaggc gcggcgggac
    54901 aggcggtgca aaatatgaac atcatgttcg gactgaagga aacacacggc ttggacgcaa
    54961 tccccctgct cccctgaaac gcaaacggca aaccgcaggc atcgtgcctg cggttttttg
    55021 atgccatctg aaagcggcgg tttttttggg ttcggacggc ttttggctca ttattccgcg
    55081 aaaacaaaaa tctaaaatac cgtcattccc gcaaaagcgg gaatctatag tggattaaat
    55141 ttaaaccagt acagcgttgc ctcgccttgc cgtactatct gtactgtctg cggcttcgtc
    55201 gccttgtcct gatttaaatt taatccacta tagtttatcc ggcttcagcg atttccgaca
    55261 catttccgca cgcttcaatt tcgtcatttc tccggtttca gtcattgccg ataacaccgt
    55321 ggttttttat ttctagattc ccgcctgcgc gggaatgacg gcggagggct tgccgttttt
    55381 cccggtaaat acctgcaatt taaaatccca tcattgccgt gaaaacaaac caaaaaccta
    55441 aaatcccatc attgccgcga aaacaaacca aaaacctaaa atcccgtcat tcccgcaaaa
    55501 gcgggaatct agtttatccg gcttcagcga tttccgacac atttccgcac gcttcaattt
    55561 cgtcatttct ccggtttcag tcattgccga taacaccgtg gtttttcatt tctagattcc
    55621 cgcctgcgcg ggaatgacgg cggagggctt gccgtttttc ctggtaagtc tctgcggctt
    55681 ctcattgccg gtttccgcct acttgggaat gacgtgattt aaaatcatga aaatgtgtca
    55741 aaaataatgc aaaatcagat ttccgttaca cttttttcca atatttcaga cggcattttg
    55801 ctcacacgcc caaataccct tccctgccgg aaagccacct tgccaaatgc gcttcgacga
    55861 tttcggggtt ttgctcaatc agcatcgggg cgatttcgcg cacttgttcc aaaaggtgta
    55921 agtcttcttc aagcttggcg aagcgcagca taggcacgcc gctttggcgc gcgccgagaa
    55981 attcgccggg gccgcggata ttgaggtctt ggcgggcgat ttcaaagccg tcggtgtgtt
    56041 cgtaaatcac tttcaaccgc gctttggcga gttcgcccaa gggttcggca aacaggagga
    56101 cgcacacgct ttctgccgcg ccgcgcccga cccgaccgcg taattggtgc agctgcgcca
    56161 agcccatgcg ctcggcgtgt tcgatgacca tcagggcggc attgggcaca tctacgccga
    56221 cttcgatgac ggtggtggcg accaagacgt tcagcccccc cgaagaaaac cgcgccatca
    56281 cttcggcctt ttcggcggcc ttcatgcgcc cgtgtaccag tccgatattg agttcgggca
    56341 atgccgtctg aagccgggcg agggtttcgg cggcggtttg cagttgcagg gtttcgcttt
    56401 cttcaatcaa tgggcagacc caatacgcct gccgcccttt tcggcaagtg ccgaggacga
    56461 agccttcgac ttcggcacgg cggacgttgt tgacgaggcg cgttttaatc ggtgtgcgcc
    56521 cgggcggcaa ttcgtcgatg acggacacgt ccaaatcggc gaaaaaactc atcgcaagcg
    56581 tgcgcgggat gggcgtggcg gacatcatca gctgatggac ttcgcgccct ttgtttttga
    56641 gggcgaggcg ttgggcaacg ccgaaacggt gctgttcgtc cacaatggtc aagcccaaat
    56701 tgtgaaacgc cacgccgtct gaaaacaggg cgtgcgtgcc gacggcgatt ttgacgctgc
    56761 cgtcagccag tttggctttg gcttcgtctt tggctttttt acgcaaactg ccaaaaaggc
    56821 ggacaacttc aatgcccaaa ggttcgagcc attgtttaaa tttaataaaa tgctgttcgg
    56881 caaggatttc agtgggcgcc attacagcca cctgcgcacc ggattcgata gccgtcaaag
    56941 cagacaaagc agccacaatg gttttgccgc tgccgacatc gccctgcagc aggcggtgca
    57001 tcgggtaggt ttgcgccata tcgcggcaga tttcggaaac aactttttct tgcgcatcgg
    57061 tcagggcaaa cggcagggct tggcgcaggg cttgggtcaa tgtgccgtcg ccgcccaatg
    57121 ccgccgccgt gccgccgata cgcttctgtc gcgccaagcg catcgaaagc tgttgcgcca
    57181 aaagttcgtc gaatttgagc cgttgccatg caggcagcgt gccgtctgaa agctgatgaa
    57241 tcgtgaaact cggcggcggc gaatgcaaaa gacgcaggct ttcggcgagg tgtggcagct
    57301 tcagacggca cagcagggca tcgggcagcg tgtcgtgcag cggcgtaacg tccaacgccg
    57361 tctgaataat acggcgcaaa gtgggctggt tcaaaccgtt tacggtcggg taaaccggcg
    57421 tgaggctttc cgccaaaccg ccgccctcgg catcgcggat tttgggatga atcatctcgt
    57481 cgccgtaaaa gccgtgtttg atttcgccca cggcgcggat gcgtttgccg accgccgtct
    57541 gtttctgatg gctggcgtaa aagtggatga agcgcagaaa aaggacgctg ccggagccgt
    57601 cggcgatttg gacaatcagc tgcttgcgcg gtttgaacgt tacttcctga tggataacct
    57661 ccccctcgac ctgacacggc acgccaatcg gcgcgtcctt aatcggcata atgtgcgtct
    57721 cgtcctcgta acgcagcggc aggtgcaaca ccaaatccca cgcggtatgg aggttgagtt
    57781 tgtcgagctt cttggcggaa acatcggtga ttttgagctg ttttcgggtt tcgggcgaca
    57841 tcataggcag attcctttgg acgcgcctat tttatccgaa aacaaaaatg ccgtctgaaa
    57901 cggattcaga cggcatcgac aggcaggaat caagccccgg cggcttcggc ttcctgctgt
    57961 tgttggtaaa tcgcctcaaa attaatcggc gcgagcagga cgggcgggaa gccggcgcgc
    58021 gttaccgtac cggaaaccgc ttcgcgcgcg taagggaaga ggatgttcgg acacgccacg
    58081 ccgagcagca ggtcggcatc ttcttcgggg atgttttcca aacggaagat gccgctttgg
    58141 gttacttcgt tcaaaaacat cgtgcgctcg ttatccagct tggcggtaac ggttacggta
    58201 acgtccacgt tgtagtagcc gtcttccagc ttttggctgc cggtggaaac gcgcatatcc
    58261 acttcgggct cgccctgttc caaaaagatt tgcggcgcgt gcggcacttc caaagacaag
    58321 tctttgacat acagtcgctc gatgctgaat acgggttgca gttcttcgct cattttgttt
    58381 tcctagttgg gggttaaggg ttcagcagtc cgtccagccc gccttcctgc tggaggcggt
    58441 agaggtcggt aaatccgccg acgtgcgttt cgccgatgaa aatctgcggc acgctgcgct
    58501 gtccggaaag ctgctgcatt tcggcaaagg cttcgggact tgcatcgaca cggatttcgt
    58561 cgatatgtcc gacacctgcc gcgtgcagca gccttttcgc catcgtgcag taggggcaaa
    58621 acggacctgt gtacatggta acggtctgca tattgggttt ccgaaagttt tgcaatgata
    58681 atcaatatag gggcatttcc cccgtttggc aagtgcggaa cagatgcacg ttcaaacggc
    58741 atgtgcggaa tgtgtcaaag tttctttttt aaagtatgat agacattgtg aaaaatattt
    58801 ttgcacccgc gctgcgcggc ggaacggatg caaaaatatt tttattacat tttcaggaaa
    58861 aaccatgttg tcaggactcc ccatccccaa agacatcgcg cgcccgcccg aaacgatatt
    58921 ggtcaacatc acgccgcagg aaacgcgcgt agcggtgttg gaggaaaaca atatctgcga
    58981 gctgcacatc gagcgcaaca gcgaacacag cctagtcggc aatatctatt tgggcgtggt
    59041 gcgccgcgtg ctgcctggga tgcagagcgc gtttatcgac atcggcttgg aacgcgcggc
    59101 gtttttacac atcgtcgatg tcctcgaaca acgccgcaac cccgaagaaa cccagcgcat
    59161 cgaacatatg ctgtttgaag ggcagtctgt tttggtgcag gtcatcaaag acccgatcaa
    59221 caccaaaggc gcgcggcttt ccacccaaat ctcgctggcg gggcgtttcc tcgtccatct
    59281 tccgcaagaa gaccacatcg gcgtgtccca acgcatcgaa gacgatgccg aacgcagcag
    59341 cctgcgcgaa cgcctcgaca agctcctgcc ggaaaatgcc tgccggggct acatcatccg
    59401 caccaacgcc gaaaacgcca ccgacgaaca gctccagtcc gacatcgact acctgaccaa
    59461 agtgtgggaa cacatccaag aacaggcgaa aatccggccg ccggaaaccc tgctttatca
    59521 ggatttgccc ttgagtttgc gcgtgttgcg cgatatggtc ggctgcgaca cgcaaaaaat
    59581 cctcgtcgat tccaccgtaa accacggccg catgacgcgt tttgccgaac aatacgtcca
    59641 cggcgcattg ggcaggatag aattgttcaa aggcgaacgc ccgctatttg aaacccacaa
    59701 cgtcgaacag gaaatcagcc gcgccctgca accgcgcgtc aacctcaact tcggcagcta
    59761 cctgattatc gaatccaccg aagcgatgac cacgatagac gtgaacacag gcggcttcgt
    59821 cggcgcacgc aatttcgatg aaacgatttt ccgcaccaac ctcgaagcct gccacaccat
    59881 cgcccgcgaa ttgaggctgc gcaacctcgg cggcatcatc atcatcgact tcatcgatat
    59941 ggcgcaggaa agccaccgcg aagccgtgtt gcaggagctt gccaaagccc tcgccttcga
    60001 ccgtacccgc gttaccctgc acggttttac cagcctggga ctggtcgagc tgacgcgcaa
    60061 acgctcgcgc gaaaacttaa accaagtcct ctgcgaaccc tgcccttcct gccaaggcag
    60121 aggccgtctg aaaacgccgc aaaccgtgtg ctacgaaatc cagcgcgaaa tcgtccgcga
    60181 agcgcgccgt tacgatgccg aaagtttccg catcctcgcc gcccccaacg tcatcgattt
    60241 gtttttggac gaagaatcgc aatccttggc aatgctgata gatttcatcg gcaaaccgat
    60301 ttctctggcg gtcgaaaccg cttacacgca ggaacaatac gatatagtgc tgatgtaaaa
    60361 aatgccgtct gaagccttca gacggcatct gtctatttca gggtttcctt gtccaacaac
    60421 gcccgtatca acagaccgtg tccgaaacgt cggctgtcgg tcagttccaa atgtccgccg
    60481 tattttttgg caagcgtgtc ggcgatggac agacccagcc ccgtcccctg ctgctccgtt
    60541 cccaaaatac ggtaaaacgg atcgaggacg cgggcgcgtt cggattcggg aatgccgttc
    60601 ccgttatctt ccacccacac ggcaagatat ttcccttcgt ctgtgaaacc caaatctatc
    60661 ctgccttcag gcggcgtgta acgtaccgcg ttatcggcaa aggttttaat cagcgtatag
    60721 atttctgttt cgtcggcaga cacttcgaca tcgcctctga ccgccacacc gatgtcctga
    60781 cgtttttcca aagccagcgg catcagttcc tgcaacactt ggcggaaacg gctttgcaga
    60841 ccgaatgtcg ttttcgtcag aggggtttcg tccgactgcg aacgcgccag tgccaaaagc
    60901 tgttcgagca ggtgtttgtt gcgcctgatg ctctgctgca aaacggcagg ctgccgcgct
    60961 gcatcgggtg ggagcgacat attgttgagc cgttccgcct gaagggaaag ggctgtcatc
    61021 ggcgtacgca attcgtgtgc cgcgtcggcg acaaaccgct gacggtggcg gatgtcttca
    61081 tccgcacgtt tcaaaagcag gttgatggcg gttacgaacc ctctgatttc actgggaata
    61141 ttgtccacac tcaaagcaga caggtcattg attcggcgtt gttcgagact ttgcgacaat
    61201 ttgcggacgg ggcgcatggc tttgtgcgta atccacacgg tcagcagtat catcagcggc
    61261 agtgccgcca gcaggggcaa cacgctttgc cgtgccgcat ccgccgccaa atcttcacgg
    61321 tattcgtttt cctgcataac ggcaatccgt ccctgttcgg tcgtgcggat atagacgcgg
    61381 taataatcgt cgtccccgtc cgcctgaagc gtgtgcagac cgtccgccag atgcgcaggc
    61441 aggctgacaa cagggtcttc ctgctgcggc atctgtacca aaatacgcgt atcgccgtcg
    61501 ccctcgggca aagtttcggg tttggaatcg ggggcgacgt acaatgccgc ctgacggagc
    61561 aggtcgtcct gcaacgcttc cgtttcgtgg aaggtttcgt agtaggaaaa catacctgca
    61621 agcattgcca gcggaacaaa catccaaacc gtccgccccg gcaaacccaa atccagcagc
    61681 atcaagtcat aaggctgggc agcggcagcc gcgcgccgtt tttgacccaa tccaccgcat
    61741 agccgccgtc tttcaaactt gccgacaccg cctccgcaat catcgcatcg tcttccacca
    61801 gcaaaacacg catcaacttt ccctttcaga caaaccgtgc ctattctaac accccaaaat
    61861 tagccgcaat ttagcggcct ttacgcttgc cggtattttt caaaactgca gcacaaaaaa
    61921 accgcgccgg caactgcctt cagacggcat tggggcgcga ttgcaacaca cgggcaggca
    61981 ggcagagcct gcgacagacc acaggaacga ttcgggcttc agacggcttc gccgtttacg
    62041 gcagaggcac gattcctgcc gctatcgaac tggccaatat cgccagcgac aaaccccacg
    62101 cccaaaaaaa cgaataacgg atgtgtttgc ccatcgacaa tttcgccaaa cccaagccca
    62161 tccacaaagc cggcgaaagc ggcgtaacaa aagtgccgac gatactgccg atcaacatcg
    62221 cataacctgc tgcttcgggc gccacgcccg cctgcgaggt aatctgctcc acaatcggaa
    62281 acagtccgaa ataataagcg tccgtactca aaaccaactc aagcggaatg cccaacacac
    62341 cgatggcaat atgcagataa ggcaacagcg cgtctggcag gatatgcaca atgtctttgg
    62401 aaatcgcgtc caacatcccc gcacccttca aaatccccaa aaacgtacct gccgccaaaa
    62461 taatggacgc catcatcacc gcgccgccgg cgtgggcata aatccgctcc atctgttcct
    62521 gcgggctgcg gtaattcaaa agcaacgccg ccgttgcagc cagcataaat acataacccg
    62581 gcgggaagat gcccgaaaaa agcaggctca tcgccgccaa aaacagcagg acattccacc
    62641 aaaacagttt cggacgcgcc aatttttgtt cttcttccga caaaggcacc ggctttatca
    62701 aatccgccac ggcgggcaac gcgcccaact cccggacaat ccgccttttt tcacgcacac
    62761 ccaaaagcag ggacagcgca aggataaaca ctacaccgat aatttgcacc gtcaacaaag
    62821 gtttatacaa ttcgcccaca tctgcgccca acacgcttgc aacccgccca gtcggcccgc
    62881 cccacggcag aaggttaatc aatcccgcac tggaagttag cagcaaaaac agcaggtaag
    62941 gattcatatg cagacgcttg taaagcggca aaagggcggg gacgaccaat aaaaacgtcg
    63001 tcgcacccgc cccgtccaac tgcgccacca ccgacaccaa gaccgtcccc acactcactg
    63061 ccacgatatt accccgagtc agcttaatca aaccgcctat catcggacgg aacagcccca
    63121 catcgttcat gattccaaaa aacaaaatgg aaaacataaa cataatcaca atctgcatca
    63181 ccgatttggt gccgcccgaa taaaattctt ttaattggga tacatcaaac cccgccagca
    63241 acgccccaaa cagcggcacc aagattaatg cgatgatggg cgacactttt tccgtcagca
    63301 gcagccatac gatgaccccg ataatcagca gtccgataaa cgtcagcatc atttctcctt
    63361 tattttattt taaacagaaa accgaccgtg caggcaaaac cgcccacaga cgcgggataa
    63421 gccctgcatt ctactttttt attttgaaac aagtcaatcg gtcatttcct cccattcacg
    63481 cctgccgcca ttcctgcatc cgtccgtcat ttcacggcgg caaccgatac ggaacaaccg
    63541 gcaaatcggt atcgggacgg cgcgggggca ttcatcccgg tgcgccgatt caaacgaaac
    63601 cgcccctatc attgcggagc gcggggcgtg ccgtacacgc gggattttat agtggattaa
    63661 caaa
//
LOCUS       DAWFIE010000004        46029 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWFIE010000004 DAWFIE010000000
VERSION     DAWFIE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 46029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46029
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.004"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..194)
                     /locus_tag="WGQ71_000205"
     CDS             complement(<1..194)
                     /locus_tag="WGQ71_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0706284.1"
                     /translation="MKITHCKLKKKVQKELLHFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLVLAADEVFE"
     gene            241..810
                     /locus_tag="WGQ71_000206"
                     /pseudo
     CDS             241..810
                     /locus_tag="WGQ71_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            1042..1542
                     /locus_tag="WGQ71_000207"
     CDS             1042..1542
                     /locus_tag="WGQ71_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706285.1"
                     /translation="MVFVNPLYYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAAL
                     FITLFFAAVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEK
                     AVRYTRRVTQLWCVFFIINGTLAALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYR
                     KLVLKV"
     gene            1549..2064
                     /locus_tag="WGQ71_000208"
     CDS             1549..2064
                     /locus_tag="WGQ71_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ0706286.1"
                     /translation="MYTCTSCGENHEEMPAIGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYTDQTDRFIRAVLPIPIIGHQETLEYGVWVSVSEKSFNDYQSRFYDNLENAVYF
                     GMICNQLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGGEHPLVRDFYHGIEYAEAQAR
                     IGAVSELDCSG"
     gene            2084..3562
                     /gene="ubiD"
                     /locus_tag="WGQ71_000209"
     CDS             2084..3562
                     /gene="ubiD"
                     /locus_tag="WGQ71_000209"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ0706287.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMVPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFHKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPMFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(3627..5741)
                     /locus_tag="WGQ71_000210"
     CDS             complement(3627..5741)
                     /locus_tag="WGQ71_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ0706288.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDRTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSNGIGPPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            5832..6725
                     /locus_tag="WGQ71_000211"
     CDS             5832..6725
                     /locus_tag="WGQ71_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ0706289.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHDAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGNLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLNHTKGHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            7051..7908
                     /gene="folP"
                     /locus_tag="WGQ71_000212"
     CDS             7051..7908
                     /gene="folP"
                     /locus_tag="WGQ71_000212"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ0706290.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRLGADYVSPEEEWARVEPVLAEVAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQADTGICLMHMQGLPKTMQINPKYQ
                     DVVGEVARYLKARSAECIAAGIAPQRIILDPGFGSGFGKTLQHNIALMRHLPELMAET
                     GFPLLIGVSRKSTIGELTGEANAAERVHGSVAAALASVARGAQIVRVHDVKATADALK
                     VWEALGINL"
     gene            8038..9372
                     /gene="glmM"
                     /locus_tag="WGQ71_000213"
     CDS             8038..9372
                     /gene="glmM"
                     /locus_tag="WGQ71_000213"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ0706291.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVIQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPTHSDLRGLKLVVDAANGAAYSVAPKVFHELGAQVVSIGDEPDGYNINEK
                     CGATHTKTLQAAVLQNEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRSWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIGKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            9561..10244
                     /locus_tag="WGQ71_000214"
     CDS             9561..10244
                     /locus_tag="WGQ71_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0706292.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            10311..11300
                     /locus_tag="WGQ71_000215"
     CDS             10311..11300
                     /locus_tag="WGQ71_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ0706293.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDTADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVRDFTRQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGAEDVEKELARLKL"
     gene            11415..12185
                     /locus_tag="WGQ71_000216"
     CDS             11415..12185
                     /locus_tag="WGQ71_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0706294.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPQQRERQIQVFSRQLEIAQTLRRRVIIHNLKAAADIAAAVKRTGFTWGGIVHA
                     FSGSIEEAHVLAKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKNTV
                     NTPANILRIAEIAAQIRGTGAAEIAAITERNADSLLRP"
     gene            12275..13351
                     /gene="prfA"
                     /locus_tag="WGQ71_000217"
     CDS             12275..13351
                     /gene="prfA"
                     /locus_tag="WGQ71_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ0706295.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKINTLDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            13476..14474
                     /locus_tag="WGQ71_000218"
     CDS             13476..14474
                     /locus_tag="WGQ71_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0706296.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAHDPFCNPEAEKIGGRYVDLDELYAQSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(14552..15847)
                     /gene="serS"
                     /locus_tag="WGQ71_000219"
     CDS             complement(14552..15847)
                     /gene="serS"
                     /locus_tag="WGQ71_000219"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ0706297.1"
                     /translation="MLDIQLLRSNTAAVAERLAARGYKFDAARFNALEEQRKAVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLEAVQKELDAWL
                     LSIPNLPHEDVPFGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            16013..16537
                     /locus_tag="WGQ71_000220"
     CDS             16013..16537
                     /locus_tag="WGQ71_000220"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ0706298.1"
                     /translation="MLDREGYRPNVGIILINNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQSSDNR"
     gene            16604..18589
                     /gene="parE"
                     /locus_tag="WGQ71_000221"
     CDS             16604..18589
                     /gene="parE"
                     /locus_tag="WGQ71_000221"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ0706299.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVSNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(18663..19088)
                     /locus_tag="WGQ71_000222"
     CDS             complement(18663..19088)
                     /locus_tag="WGQ71_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ0706300.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(19179..20279)
                     /gene="aroC"
                     /locus_tag="WGQ71_000223"
     CDS             complement(19179..20279)
                     /gene="aroC"
                     /locus_tag="WGQ71_000223"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ0706301.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRRETDQVEILSGVFKGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            20422..21525
                     /gene="trmA"
                     /locus_tag="WGQ71_000224"
     CDS             20422..21525
                     /gene="trmA"
                     /locus_tag="WGQ71_000224"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ0706302.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETH
                     AVERAALFDQFPFTHHIESGVLLKKKILGKSKR"
     gene            21531..22724
                     /locus_tag="WGQ71_000225"
     CDS             21531..22724
                     /locus_tag="WGQ71_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0706303.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAATPVPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFESAGFEVGTYPYYDPAAVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(22789..22864)
                     /locus_tag="WGQ71_000226"
     tRNA            complement(22789..22864)
                     /locus_tag="WGQ71_000226"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22829..22831),aa:Arg,seq:ccg)
     gene            23149..23988
                     /locus_tag="WGQ71_000227"
     CDS             23149..23988
                     /locus_tag="WGQ71_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0706304.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            complement(24453..27305)
                     /gene="gcvP"
                     /locus_tag="WGQ71_000228"
     CDS             complement(24453..27305)
                     /gene="gcvP"
                     /locus_tag="WGQ71_000228"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ0706305.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEQSMDDFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSGRFFVDERVYPQTLDVMKTRAKYFGFELVVGDFAKADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVSADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLVGMYAVYHGPEGVKRIAKRIHALASAFADALVSDGIKVVHKVFFDTVTFDFGSK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSAYEDLVDLYRAFTGKDTFTFADDVK
                     GRLNAELLRQDDILQHPVFNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEVQTAGYRELLTDMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLSIRRYQESQGEAHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHNAAADQT
                     AVAAAAYGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGNWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(27336..27695)
                     /locus_tag="WGQ71_000229"
     CDS             complement(27336..27695)
                     /locus_tag="WGQ71_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706306.1"
                     /translation="MTDRPNLLRIKPGQTEIMKYCEPEKASDPYATYRRANLMAVLPL
                     FVVILVLLNIVFPLPAHPLAWLVLEGFMVLVGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCYPYPNHPMSKNSA"
     gene            27643..27873
                     /locus_tag="WGQ71_000230"
     CDS             27643..27873
                     /locus_tag="WGQ71_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0706307.1"
                     /translation="MISVCPGFIRSRFGRSVMPSEPAGRLFQKTPYRCNFMRLYGVGF
                     ALQTAYASRSSLVFQSVHDYINNLGADKPLFI"
     gene            complement(27866..28507)
                     /locus_tag="WGQ71_000231"
     CDS             complement(27866..28507)
                     /locus_tag="WGQ71_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ0706308.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARAHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(28497..29048)
                     /locus_tag="WGQ71_000232"
     CDS             complement(28497..29048)
                     /locus_tag="WGQ71_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ0706309.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIDSDNGLFERLVLEI
                     NQAGLNWTLMLKKQQAFQTAFKGFDIDTVAAFGEADIERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(29052..29570)
                     /locus_tag="WGQ71_000233"
     CDS             complement(29052..29570)
                     /locus_tag="WGQ71_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ0706310.1"
                     /translation="MRLFPIAAALSLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(29570..31231)
                     /gene="pqiB"
                     /locus_tag="WGQ71_000234"
     CDS             complement(29570..31231)
                     /gene="pqiB"
                     /locus_tag="WGQ71_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ0706311.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGVKLNSAPLPALLSGAISFDSPKTSDSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLSAGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFGKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRITLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(31224..32558)
                     /locus_tag="WGQ71_000235"
     CDS             complement(31224..32558)
                     /locus_tag="WGQ71_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ0706312.1"
                     /translation="MPPLIFSTFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCR
                     ADVPRLDKGEAAFCPRCGHKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPG
                     AASVLSLPEMVRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKRVYPALR
                     LATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSV
                     PQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLS
                     ISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSAS
                     ILVPVLKIAAMSVLITSARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFH
                     TYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            32857..33486
                     /locus_tag="WGQ71_000236"
     CDS             32857..33486
                     /locus_tag="WGQ71_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ0706313.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            33661..36036
                     /gene="hmbR"
                     /locus_tag="WGQ71_000237"
     CDS             33661..36036
                     /gene="hmbR"
                     /locus_tag="WGQ71_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
                     /protein_id="HEZ0706314.1"
                     /translation="MKPLQMLPIAALVGSIFGNPVFAADEAATETTPVKAEVKAVRVK
                     GQRNAPAAVERVNLNRIKQEMIRDNKDLVRYSTDVGLSDSGRHQKGFAVRGVEGNRVG
                     VSIDGVNLPDSEENSLYARYGNFNSSRLSIDPELVRNIEIVKGADSFNTGSGALGGGV
                     NYQTLQGRDLLLDDRQFGVMMKNGYSTRNREWTNTLGFGVSNDRVDAALLYSQRRGHE
                     TESAGNRGYAVEGEGSGANIRGSARGIPDPSKHKYHSFLGKIAYQINDNHRIGGSLNG
                     QQGHNYTVEESYNLLASYWREADDVNRRRNANLFYEWTPDSSWLSMVKADVDYQKTKV
                     AAVNNKGSFPMDYSTWTRNYNQKDLDEIYNRSMDTTFKRITLRMDSHPLQLGGGRHRL
                     SFKTFASRRDFENLNRDDYYFSGQISRTTSSIQHPVKTTNYGFSLSDQIQWNDVFSSR
                     AGIRYDHTKMTPQELNAECHACDKTPPAANTYKGWSGFVGLAAQLNQAWRVGYDITSG
                     YRVPNASEVYFTYNHGSGNWLPNPNLKAERSTTHTLSLQGRSEKGMLDANLYQSNYRN
                     FLSEEQKLTTSGTPGCTEENAYYGICSDPYKEKLDWQMKNIDKARIRGIELTGRLNVD
                     KVASFVPEGWKLFGSLGYAKSKLSGDNSLLSTQPLKVIAGIDYESPSEKWGVFSRLTY
                     LGAKKVKDAQYTVYENKGWGTPLQKKVKDYPWLNKSAYVFDMYGFYKPAKNLTLRAGV
                     YNLFNRKYTTWDSLRGLYSYSTTNAVDRDGKGLDRYRAPGRNYAVSLEWKF"
     gene            36408..36656
                     /locus_tag="WGQ71_000238"
     CDS             36408..36656
                     /locus_tag="WGQ71_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ0706315.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLIGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
     gene            36653..37039
                     /locus_tag="WGQ71_000239"
     CDS             36653..37039
                     /locus_tag="WGQ71_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ0706316.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(37644..38120)
                     /locus_tag="WGQ71_000240"
     CDS             complement(37644..38120)
                     /locus_tag="WGQ71_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ0706317.1"
                     /translation="MLAACQSSRAPKNAHTASAGTPAGESCKVIRGDTAGSGKNIIYR
                     CSNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGVGKSDSEACERA
                     LINAARKFQQTAGKLGGRSVTGFHSYFDKQPLQGGQYDCQTGSFHVRVVMRANVVR"
     gene            complement(38253..39608)
                     /gene="yegQ"
                     /locus_tag="WGQ71_000241"
     CDS             complement(38253..39608)
                     /gene="yegQ"
                     /locus_tag="WGQ71_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ0706318.1"
                     /translation="MKAPELLLPAGGLERMRTAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDENGWATVEVKNR
                     FAVGDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            39933..40721
                     /locus_tag="WGQ71_000242"
     CDS             39933..40721
                     /locus_tag="WGQ71_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ0706319.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREMPMDVGQMGRACSFLDGL
                     GMDYAFVGGRGIVVSALSECVCRALKHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGASKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCHSVDEDGVLRGLQDLGVI"
     gene            complement(40767..41366)
                     /locus_tag="WGQ71_000243"
     CDS             complement(40767..41366)
                     /locus_tag="WGQ71_000243"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ0706320.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            41494..42111
                     /gene="gmk"
                     /locus_tag="WGQ71_000244"
     CDS             41494..42111
                     /gene="gmk"
                     /locus_tag="WGQ71_000244"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ0706321.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLAKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLVHIVNACRLKRSRQLGFIADLLENS"
     gene            42170..42376
                     /locus_tag="WGQ71_000245"
     CDS             42170..42376
                     /locus_tag="WGQ71_000245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ0706322.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            42468..44624
                     /locus_tag="WGQ71_000246"
     CDS             42468..44624
                     /locus_tag="WGQ71_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0706323.1"
                     /translation="MPAPQPSAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATLVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(44691..45875)
                     /gene="coaBC"
                     /locus_tag="WGQ71_000247"
     CDS             complement(44691..45875)
                     /gene="coaBC"
                     /locus_tag="WGQ71_000247"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ0706324.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILKGKKVLITAGATFEAIDPVRGITNISSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAENMHRAVHRLIDKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKRLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
BASE COUNT        10790 a        11670 c        12775 g        10794 t
ORIGIN      
        1 tcctcaaaaa cctcatcggc agccaagacc aaatgatggt tgataaccgt gcggatttta
       61 cggtagaaca gtgctgccga attgggatgg atacccaaaa tatcggcggc agaacgggcg
      121 gtaacttcca gtacaaaaaa atggagcagt tctttctgta cttttttctt taatttgcag
      181 tgtgttatct tcatatttcg agggtaacat atctgctaat ctagtaaaat ttaatccact
      241 atagtttatg cctcgcacga cggggaaatg gcgcgcagtt tcgatttgtg gttggaactg
      301 ttgaaatcgc aaatcgtgtc gccgacttcg ttcggtttgt cggtgcacaa tgcaacggcg
      361 gggtaatggt cgatattgag gcgggatatg agcgagcaga cggcattggc cgtgtgtgcc
      421 gacggtgtgg aaacggcatt ggcggaagcg gcatcactgt tggaagaggg atgcgggtcg
      481 gttttggttt tggcggcgga cgatccgctg ccggaaggat atgccgtatc ggcaacgcgc
      541 gcgccgtttg cttatgcttt ggcgatggtt ctgaccaaag ggacgcgtta cagcctgact
      601 ttatctgctt cggacgatat gccgtctgaa gccggtatgc tgcccgaggc atattggagc
      661 ggtttggaat gggtgcgctt tctgctgaac ggcagtcggg aatgccgccg ggtatatcgc
      721 aatcgtgaat ggctgtggca gcccgcaagc tgcaggctga agatttccgc aatgtccgca
      781 ccgtcaacga cgtggtacag gcggtattga agattcaggc gggctaatcg ggtatttcta
      841 attatttttc agacggcatg tttttatcgg atgccgtctg aaaacatttg tgcggattca
      901 aaatgaaacc cggttttttc ggacatattt ttctgaccgt attaagcatt gcctatcccg
      961 ctttgtggta tagtggatta acaaaaatca ggacaaggcg acgaagccgc agatagtacg
     1021 gcaaggcgag gcaacgctgt actggttttt gttaatccac tatattacgg cagggaggcg
     1081 ggctggtttg tctggctggc ggcggcgatg tgcgggttgt ggctgctgcg cgccctgact
     1141 gccaaactgc cccaacagcg ttatgcggct ttgtttatta cactgttttt tgccgcagta
     1201 ttggtgttcg ggcggcatga ttcgatgtat tggtatcccg tcctcgtcaa tctgatgatg
     1261 ttggcggtgt tcggcggcag cctgtttgcc gggcagacgg tgattgaaaa acttgcgcgg
     1321 ctgcaacagc ccgacctgcc tgaaaaagcc gtgcgctata cccgccgggt aacgcagctt
     1381 tggtgcgtat tttttataat caacgggacg cttgccgctt tgttggcacg gttggggcgg
     1441 tatgattggt gggcggttta tacaggcgtg attgcttatg tattgatggg gatgctgttt
     1501 gccggcgaat ggctttaccg caaactcgtt ttgaaagtct gaaaccttat gtacacttgc
     1561 acttcctgcg gcgaaaacca cgaagaaatg cccgcaatcg gctttaccgc cccctatccc
     1621 tataatcaac tcagtgaaga agaacgcatt gcttatcaag cggaatgtga ttctgatttc
     1681 tgcatcatcc gctataccga ccaaaccgac cgctttatcc gcgcggtgct gccgattccg
     1741 attatcgggc atcaggaaac actggaatac ggcgtgtggg tatccgtcag cgaaaagagt
     1801 tttaacgact atcaaagccg cttttacgac aaccttgaaa acgctgttta tttcggcatg
     1861 atctgcaacc agctgccccc ttatgaaacc gacaccttcg gtctgcactg caatgtcgtt
     1921 acccaacccg atggtcaacg tcctttgttg caactccatc aaggcggcga acatccgctg
     1981 gtacgcgact tttatcacgg catagaatat gccgaagcgc aggcaaggat aggggcggta
     2041 tccgaattgg attgttcagg ataaaaggac aaaaaggata aatatgaaat acaaagacct
     2101 gcgcgacttc atcgctatgc tcgagcagca gggcaaactc aagcgcgtcg cgcatcctgt
     2161 ttccccgcat ttggaaatga ctgaaatcgc cgaccgcgtg ctgcgcgccg aagggccggc
     2221 attgttgttt gaaaacccag ttaagcccga cggtacgcgc tacgattatc ccgtgttggc
     2281 aaacctgttc ggcacgcccg aacgtgtggc aatgggcatg ggtgcggaca gcgtgtccaa
     2341 gctgcgcgaa atcgggcaga cgctggcgta tttgaaagaa cccgaaccgc ccaaaggcat
     2401 caaagacgcg ttttccaaac tgccgctctt gaaagacatt tggagcatgg tgccgaacgt
     2461 ggtgaaaaac gcgccgtgtc aggaaatcgt gtgggaaggc gaagacgttg atttgtatca
     2521 acttccgatt cagcattgct ggccggaaga cgttgcgccg ctggtaacat ggggcttgac
     2581 cgtcacgcgc gggccgcaca aaaaacgcca aaatctcggc atttaccgcc aacaactcat
     2641 cggcaaaaac aagctgatta tgcgctggct gtcgcatcgc ggcggcgcgt tggattatca
     2701 ggaattccac aaactcaatc ccgatacgcc gtatcccgtc gccgtcgtac tcggctgcga
     2761 ccccgccacc attttgggcg cggtaacgcc tgttcccgat accttgagcg aataccagtt
     2821 tgccggactg ctgcgcggtt cgcggacgga gctggtgaaa tgtatcggca acgatttgca
     2881 agtgcctgca cgcgccgaaa ttgtgttgga aggcgtgatt catccgaacg aaaccgcgct
     2941 ggaaggccca tacggcgacc acaccggcta ttacaacgag caggaccatt tccccatgtt
     3001 tacggtcgaa cgcatcacca tgcgcgaaaa cccgatttac cattcgacct acacaggcaa
     3061 accgcccgat gaacctgccg ttttgggcgt ggcattgaac gaagtgttcg taccgctctt
     3121 gcaaaagcag tttcccgaaa tcaccgattt ctacctgccg cccgaaggct gctcctaccg
     3181 catggcggtg gtgagcatga aaaaacagta cgccggacac gccaagcgcg tgatgatggg
     3241 ctgctggtcg ttcctgcgcc agtttatgta taccaaattc atcatcgtgg tggatgacga
     3301 tgtgaacgtg cgcgactgga aagaagtcat ctgggcggtc accacgcgca tggaccccgt
     3361 gcgcgacact gttttggtag aaaacacgcc catcgattat ctcgacttcg ccagccccgt
     3421 cagcggactc ggcggcaaaa tgggtttgga tgcgaccaac aaatggccgg gagaaaccga
     3481 ccgcgaatgg ggacgcgtca tcaaaaaaga ccctgcggtt acggctaaga ttgatgggat
     3541 ttgggaggaa ttggggttgt aggttttacg gtcatgccgt ctgaagcgtg ggggtgtttc
     3601 agacggcatt tgtgtacgca ggatgtttac ggttctttag gtttgagcca ctgccattgt
     3661 tccagcaggg tgtcttcgag gattttctgt ttctctttgc gcgaattgat gccgccggtc
     3721 agcctgccgt aatcgacggt aatggacggc ttgtccaccg taccggtgat tttcagggga
     3781 atcggtttgt ttttcggatg gacggcgttg cggataagga catcttcaga caattcctgc
     3841 gtatccagat tggtatagcc gttgctggta acatagaggc tgtcggagaa gagttcggta
     3901 tcgatgtggc ggctgatgcc gtctgaaatt tcgctgttga gcgtgaatcg gtggaagggt
     3961 gtgctgggtg cggcattgtc ggcagttttg cccgaaatgc cgtttttcag gatattgtcc
     4021 atgtcgatac cgtgccacgc accgttggaa atatttagcg acaggctgcc ttgaagcgag
     4081 cggataagct cttttcgggt ttcgccgccc gcggtcaggt cgatgaccgc gtcgccgttg
     4141 ccgctgaagc tgtggaagcc gaacaggtct tgcagcagcg gttggatttg gatgttgctt
     4201 gcattctgtt gcaggcggta agtggcggga cgggtgttgg cgatgctgat gccgccttcg
     4261 gtatggccgc cgtaaagccc tgacttgaaa cggctgagcg cgatatggtc tttgtcggcg
     4321 tggaggtagg tttccatatc gtccagttgc aggccgggaa gttggacttt tccgattttc
     4381 aggtgcgcct cgatgtcgcc ggacagcttg gcgagggtgt cgggaaatat tttgccgttt
     4441 tgttgccgca cgtcgtcaag atagggggtc aggttcaatt tttgcagtgc gacggcggct
     4501 tccagatgcg gtgcgtcttc atgtgtgtat ctgaatttcg cggcaacggt ttggcggtcg
     4561 aatgtgccgt ttaattcggc attccaattt tgcagattcg gtacggacag cgaaccgtcg
     4621 agccggctga tgaaacgggg ttgcggcagg cggttgacgg tatcctgaag ggtcgataca
     4681 tacaggcgcg gcgcgtccag ccctttgttt tccgtccata cgagcggcga attgagggag
     4741 aagttggtct ggtggcgcgg tgttttgaag ctgccggaga tttcggcgtt gccgatgttg
     4801 gcgatgccgg agtgcaggtt ggctttgtcg agtttgaacg aaccgtccca ttgggcatat
     4861 tcgccgccgg cggtaaatgc gccgttgacg gtttcaattt taatgctgtt gttcctgagt
     4921 gccagcgtag ggatttgggc ggtcaggtgg aggttacgga aggaagtgtc tgcacgcagg
     4981 ccgaggccgg cggcatccgc tccgccggcg ttgaagcgga cagaagggct gccggtggtg
     5041 gaaatggtaa tgccgtgtcc gtccagcgaa gtggaagctt caaaatggaa cggtgagatt
     5101 tcgggcgggc cgatgccgtt tgaaaggaac agccccctgc ttttccacgg gacggacagc
     5161 tttccccaaa ccagtatgcc cgaactttca aacggctgcc ccgacgaatc gggggattgc
     5221 aggttgaggt tgatttcctt caggataagc tgttcctgca ggaaattgag gcggacggtg
     5281 ctgttttcga cgataatgcg gttgactgag gcttggcgtt tttggctgtc gatcaggtct
     5341 tgaatgttcc aaacaccttt cccgtcgcgc gtcagggcaa gttccgcact cgaaaccacc
     5401 catttttcaa tctgtatctg atccgaccac aggtttttcc agctcaatcc gattttggtt
     5461 tcttggacgg aaacggcagt ccggtcgccg ccgggttcgg taatggtcag gtttttcagg
     5521 atgacggtcg gccggggcag gagcctgcgc tgaatgtccg catcaaacga gattttccgg
     5581 tgtgtgtgtg caatgctttg ttggaggcgg ctgcggatgt tttcaggcgt gaaggtgcga
     5641 tatacggaag cgtgcagccc gactgccgcc aaaagcagta tcgtcagcac ggccaccgca
     5701 tatttcagac ggtatttgtg gaaaaccgat aataaatcca tagcccgaac aatctgtaaa
     5761 acacgccaca gtataacacg gcttgcaaaa atcctatttt gggcaggccg tttcttgtgt
     5821 caggaggaaa tatgattatt gtcatgagca ggcgtgccgc cgaagcggac attgccggcg
     5881 ttgtcgcctt tatccgcagc agaggtctgc gagaacacat ttctcacggc gacgagcgta
     5941 ctgtcatcgg cgcaatcggc gacgataggg ttttgagcgt cagggaggtg cagacgctgc
     6001 cggaggtcga gaaagccgtc cgcattttgg atacatggaa aaccgtcagt cgggaaaatc
     6061 gggcggaaga cagccgcgtt gccgccaaag gcgtggcttt cggcggcggg gaaaccgtcc
     6121 gtatcgccgc agaaccgtca gtatggcgca atgcggatgc agtgtttctg gatccgtttt
     6181 tcacttcggc aaacttatac gacacttcaa gtgcggatga agggcgggga cggtgcagac
     6241 ggttggcgga acagaccgca tcggcgcatg atgcagggaa acccgtattg gtgcgcgtcc
     6301 gcaacgtccg ccatgtcgaa gcggctttga atgcgggggc ggacatcctg tatttgggcg
     6361 gcgggctgat gtccgatctt gccgtattga atgaagccgg aaacctgaac attcccttgg
     6421 tgctgtgtaa agacaagcac cattctgccg aagattggtt gaatgccgca gaatatgtcg
     6481 tctcgcgcgg caaccgccac ctgattctgg gcgagtcggg cgtattgaac cataccaaag
     6541 gccatcccta ccgtttggat gtcgaatcca tcgtgaaagt ccgccaaatc agccatctgc
     6601 ccgtgattgc gaatattacg ggattatgga accgcgatat gccgcaggaa atcctgtacg
     6661 gcttggcaaa ggcggcaggc gcgtgcggaa tcgtcggaac ctgttttgaa aagtcgggcg
     6721 gatagcaggc ataccggcag attcaaaggc ggagcagaca ttcggacaac atgccgtctg
     6781 ccgcccgaat agcggtgccg tctgaagcgc acaagacggt ttcagacggc atataatctg
     6841 agacctttgc aataacatag gttactaaaa ttttatgctc aatctcattt tcaaaatgca
     6901 aaacttttct gatttttcct actttttgct caatattagg aaggttttag gcaattgaaa
     6961 attttttggc gcatttttat gcgtcaaatt tcgttaacag actatttttg caaaggtctc
     7021 aatctgtttt ttattttggg aggagttcag atggcacgac acgtttggca ggcaggacgg
     7081 tttgaaatcg gtttggacaa accgaaaatc atgggtatcg tgaatctcac gcccgattct
     7141 ttttccgacg gcggagcgta ttcgcaaaac gcccaaacag ctttggcgca tgccgaacgg
     7201 cttttgaaag agggtgcgga cattctcgac atcggcggcg aatcgacgcg gctgggtgcg
     7261 gattatgttt cgcctgaaga agaatgggcg cgggttgagc ctgtattggc agaagtggcg
     7321 gggtggggcg ttcccgttag tttggacacg cgccgcacgg tggttatgga aaaagcgttg
     7381 gcactcggcg gcatcgatat tattaacgat gtggcggcgt tgaccgacga aggcgcggtc
     7441 gaattgctgg cgcgtcaggc ggacacgggc atttgcctga tgcacatgca gggtttgccc
     7501 aaaaccatgc agattaatcc gaaatatcaa gatgttgtcg gtgaagtagc gcggtatttg
     7561 aaagcgcgct cagcggaatg tatcgcggca ggcatcgcac cgcaacgcat catactcgac
     7621 cccggcttcg gctccggctt cggcaaaacc ttgcaacaca atatcgcgct gatgcggcat
     7681 ttacccgaat tgatggcgga aacaggtttt ccgctgctca tcggcgtatc gcgcaaaagc
     7741 acgataggcg agctgaccgg cgaggcaaat gcggcggaac gcgtacacgg cagcgtggcg
     7801 gcagcgttgg cttccgtagc gcgcggcgca caaatcgtgc gcgtgcatga tgtgaaggca
     7861 acggcggatg ctttgaaggt gtgggaagcg ttgggcatca atctgtaaac agcggttcac
     7921 atacttcatg ccgtctgaaa aggacggggc ggcttcagac ggcatcaacg gtgcagacag
     7981 caaggtacaa acagactgta taatctcgga caacggttca cattctcgga gcaacacatg
     8041 gcaaaaaaat atttcggcac ggacggcgtg cgcggcgaag tcgggcagtt tccgattacg
     8101 cccgattttg tattgaaact cggctatgcg gcggggcagg ttttggttca gcacgacacc
     8161 gaccagaaac cgaccgtttt gatcggcaag gatacgcgta tttccggtta tatgctggaa
     8221 gccgctttgg tggcaggttt taccgccgca ggtgtcaacg ttatccaaac cggtccgctg
     8281 cctacgcccg gcgtggctta tctgacccgt gcgctgcgtt tgtccgccgg tgtgatgatt
     8341 tccgcgtcgc ataatgccta ttccgacaac ggcatcaaat tctttgccga gggcggcgtg
     8401 aaactgagcg atgaaatcga gttggaaatc gaagccaaaa tcgacgaaga aatgaaaacc
     8461 cagccttctg cacgcctcgg gcgcgcacgc cgcatcagcg gtgcggacga ccgctatatc
     8521 gaattttgca aatccacctt ccctacgcat tccgatttgc gcgggctgaa gctggtcgtc
     8581 gatgccgcca acggcgcggc ttacagcgtc gcacccaaag tgtttcacga actgggcgcg
     8641 caggtcgtca gcatcggcga cgaacccgac ggctacaata tcaatgaaaa atgcggcgcg
     8701 acccatacca aaaccctaca ggccgccgtg ttgcaaaacg aggcagacta cggcatcgcc
     8761 ttggacggcg acggcgaccg cctgatgatg gtcgataaaa acggtaaggt ttacgacggc
     8821 gacagcctga tttacgtcat cgccaaagcc cgcgcccgag aaggtatcaa catcggcggc
     8881 gtggtcggca cggtcatgac caatatggcg atggaaatcg ccctgaaaga gcagggcgtc
     8941 gatttctgcc gcgccaaagt cggcgaccgc tatgtgttgg aacagctgaa ccaacgcagc
     9001 tggctcatcg gcggcgaagc cagcggccat attttgtgta tggacaaaca caacaccggc
     9061 gacggcatta tttccgcact gcaagttttg gcggcactgc aaacgctgaa ccaagacctc
     9121 gccaccgttt gcgccgattg gcagccgtat ccgcaaacga tgattaatgt ccgtatcggc
     9181 aaagggcaaa actggcagga agcctcaaaa gacgtgttgg cggaagtgga aaaagaactc
     9241 gaaggcaaag ggcgtgtcgt gttgcgcgct tcgggaaccg aacccgtcgt gcgtgtgatg
     9301 gtggaggcgc ggcaggcgga ttgggcgcgc gacggtgcgg aacgtattgc ttccgccatc
     9361 ggcagccttt aggcaaaatc aggcaaaacc gtttcaacag cggcggtttt cctgtttaaa
     9421 tgccgtctga acgtccggca gagggcgcga atctgcccct tgccgctttc agacggcata
     9481 atctttgata gaatgccgtt tcgcgcagaa aatgtttcgg cttgttctga ctttttatct
     9541 ccgacaatgg aatatccgct atgtttgccc ttttggaagc cttttttgtc gaatacggct
     9601 acgcggccgt gtttttcgtt ttggtcatct gcggtttcgg cgtgccgatt cccgaggatt
     9661 tgaccttggt aacaggcggc gtgatttcgg gtatgggtta taccaatccg catattatgt
     9721 ttgcagtcgg catgctcggc gtgttggcgg gcgacggcat catgttcgcc gccggacgaa
     9781 tttgggggca gaaaatcctc aagttcaaac cgattgcgcg catcatgacg ccgaaacgtt
     9841 acgcacaggt tcaggaaaaa ttcgacaaat acggcaactg ggtgttattt gtcgctcgtt
     9901 tcctgcccgg tttgcggact gccgttttcg ttaccgccgg catcagccgc aaagtatcgt
     9961 atctgcgctt tctgattatg gacgggcttg ccgcgctgat ttccgtgccc gtttggattt
    10021 acttgggcga gtacggcgcg cacaacatcg attggctgat ggcgaaaatg cacagcctgc
    10081 aatccggcat cttcatcgca ttgggcgtgc tggcggcggc gctggcttgg ttctggtggc
    10141 gcaaacgccg acattatcag ctttaccgcg cacaattgag cgaaaaacgc gccaaacgca
    10201 aggcggaaaa ggcagcgaaa aaagcggcac agaagcagca gtaaatcaga ttttcgaccg
    10261 tccggacgca tttcgcgttt caggcggtct ttgcattttc aggatgagcc atgaaacaga
    10321 aaatttttgt cctttatacc ggcggtacca tcggcatgac gcaaagcagc gaaggcctgc
    10381 gccccgatac cgcgctggtc gggcaggcac tcgcgccgtt ttcaaacggc atggatttcg
    10441 attggcacgt ttgtgggacg ctgattgatt cttccgccgt ccaactgcaa aactggtgcg
    10501 cttggctgga catcattgcc gccgcgctgc cgcagtacga cggcgttttg gtgctgcacg
    10561 gcacggacac gctcgcctat accgccaatc tgttcgccct cgcgctgcag ggtttggaca
    10621 agccaatcgt cctgaccggc tcccaatggc cgtacaacgc cgtgggcagc gacgcgccgc
    10681 gcaatctggc gactgccgtt gccgcattcc cactcggatt gaagcagacg cttattgcct
    10741 ttgacggcaa actttatccc gccgtcggca gcagcaaagt cagcaccgaa accgccgccg
    10801 gtttcgacaa tccgcatttc ggcgcactgg cggagtggga cgaaacccag ggctggcaca
    10861 atgtccgcat cccgaatcag gacacggcag atgtttcaga cggcctcaaa atccgttatc
    10921 ccgacccgca ggcaaaaatc gccgtccgca cgcttatccc cggctttgcc gtccaagaac
    10981 ttgccgacgg actcggacag cttcccgccc acgcccttat cctgcaaagt tacggacacg
    11041 gcaacacgcc cgcagacgaa ggctttatcc gcgctgtccg agacttcacg cggcaaggaa
    11101 aattgttgct caacatcagc caagtccgac aaggcagaac tgccgccgtt tacgcgcaag
    11161 gcaatgcgtt ccgcaactcg ggcatcatca acggcggcaa atgcaacctc gaaaccgcca
    11221 ccgcgctgat gaccctcgcc gtatcgcaag gctggggcgc ggaagatgta gaaaaagagt
    11281 tggcaagact gaagttatag cggattgaaa tggcaatgac ggggcgttga ccgcgcctta
    11341 tgcgccgccc gtacacggca tcccttgccg ccctgcctta tttttatgtg aacctgccgc
    11401 attgcggaat cgaaatgaac ttcaccgaca cccactgcca ccttgccgat gccgccctgc
    11461 gcgaaaacct gccgcacatc ctgaccgccg cgcggaaagt tggggtgaag cggtttatcg
    11521 ttcccgccac ctgtccgcag gattggcaaa gcgtggcgga actgtcggaa atgccgtccg
    11581 aacacggtca aatccgtatt gcgctcggca tccatccttg gttttcagac ggcattgccg
    11641 agcgtgattg cgtccgcctg gaagcgatgt tggcacgtta tccgcaggca tgggtcggcg
    11701 aaatcggctt ggatttttac gacaaaaccc aaacgccgca gcagcgcgaa cggcaaatcc
    11761 aagtcttcag ccgccagctt gaaatcgcgc aaaccctgcg tcggcgcgtg attatccata
    11821 atctcaaagc cgccgccgac atcgccgccg ccgtcaaacg gacgggcttt acctggggcg
    11881 gcattgtcca cgccttttcc ggcagtattg aagaagcgca cgtgttggcg aaactgggtt
    11941 tcaaaatcgg catcggttcg ctgctgctca acccgaacgc gcgtaaagtg cgcgatacgc
    12001 ttaaagcctt gaacgacggg gatttcgtat tggaaacgga tagcccgttt atgttgaaaa
    12061 acaccgtcaa tacgcctgcc aacattcttc ggattgccga aattgccgcg cagatacgcg
    12121 gtaccggcgc ggcggaaatc gccgcgataa cggaacgcaa tgccgacagc ctgctccgtc
    12181 cctagattat ctttttgaat attcagaaaa tatattataa tgaccgcggt ttgatgccgt
    12241 ctgaaattca ggcggcgttt gttatggaag aattatgaag ccgtctatct tagaaaaact
    12301 gcaacaactc ggcgaccggt tggaagaagt tacccacctt ttagggcaac ccgaagccac
    12361 atccgatatg gacaactacc gcaagctcac gcgcgaacac gccgaactga cccccgtcgt
    12421 cgaagtattc caaaactatc aactggcgca aagcgacttg gcagatgccg aagaaatgct
    12481 gtccgacccc gaaatgaaag actttgccgc cgaagaaatc gaagcggcaa aagccaaaat
    12541 caacacgctc gataccgaac tgcaaaaact gctgctgccc aaagatgccg acgacgacaa
    12601 aaacatcttc atcgaaatac gcgccggaac gggcggcgac gaagccgcgc tgtttgccgg
    12661 cgatttgctg cgtatgtaca gccgctacgc cgaacgcaac cgctggcagg ttgaaatcgt
    12721 ttccgccaac gaaagcgagt tgggcggcta taaagaagtc atcgcccgta ttgtcggact
    12781 cggggcgtac agccgtttga aatttgaatc gggcggacac cgcgtgcagc gcgtccccgc
    12841 caccgaaagc caaggccgca tccatacttc cgcctgcacc gttgccgtca tgcccgaagc
    12901 ggacgaactg gaagacatcg agttgaaccc cgtcgacctt cgcatcgaca ccttccgcgc
    12961 atccggcgca ggcggtcagc acatcaacaa aaccgattcc gccgtccgca tcacccacct
    13021 gcccaccggc atggtggtcg aatgccaaga cggccgcagc caacacgcca acaaagcgca
    13081 ggcaatgaaa gttctcgccg cccgcctgaa cgacgcgcaa aaacgcgaag cccaagccaa
    13141 agaagccgcc gaacgcaaat ccctcatcgg cagcggcgac cgcagcgaac gcatccgcac
    13201 ctacaactat ccccaaggca gggtaacaga ccaccgcatc aacctcaccc tgcacaagct
    13261 ggattttgtg atggacggcg atttggcaga aatcaccgat gccctgattg ccgaacatca
    13321 ggcggagctt ttggcggcaa tgggcgattg agttttgtgt tttgaaatgg aaatgccgtg
    13381 catgcaatca attttaatcc accatattct actgatttgt ttgaattcag tgtattatgc
    13441 cgtttctcaa ccatagacga caactggaca tattgatgaa gattgcgatt tacggtacga
    13501 aaagctatga ccgcgaacat tttacccgcg ccaaccggca tttcggcttc gagctggagt
    13561 tttttgattt tatgctggat gcgaaaacgg cgaaaatggc ggaaggcgcg gaagcggttt
    13621 gtatttttgt aaacgacgac ggcagccggc ccgtgttgga aaaattggcg caaattggcg
    13681 tgaaaactgt ggcgttgcgt tgtgccggct tcaataatgt ggatttaaag gcggctgaag
    13741 agttgggctt gaaagtcgtg cgcgtgcctg cctattcgcc cgaatcggtt gcggaacata
    13801 cggtcggtct gatgctgact ttgaaccgcc gtatccacaa agcctatcag cgtacccgcg
    13861 atgcgaattt ttcgctggaa ggtctgaccg gtttcaatat gtacggcaaa acggcgggcg
    13921 ttatcggcac ggggaaaatc ggtattgcaa ccatgcggat tttaaagggt ttcggcatga
    13981 acctgctggc tcacgatccg ttttgcaacc ccgaagcgga aaaaatcggc ggcaggtatg
    14041 tggatttgga cgagctgtat gcccagtcgg acatcatcac gctgcattgc cccgccacgc
    14101 cggaaaacca ctatatgctg aacgaagcgg cgttcgacaa aatgaaagac ggcgtgatga
    14161 ttatcaacac cagccggggc gggctgattg acagtgccgc cgcgattgag gcgttgaaac
    14221 gccggaaaat cggcgcattg ggcatggatg tgtacgagaa cgagcgcgag ctgttttttg
    14281 aagacaaatc caacgatgtg attaccgacg acgtgttccg ccggttgtcg tcttgtcata
    14341 acgtgctgtt taccggtcat caggcgtttt tgacggaaga ggcgttgggt aatatttcgg
    14401 aagttacgct gtccaatatc cgcgaggtcg gacagaccgg cgattgcggc aatgcggttc
    14461 gtgccgacgg ctgacgtgtt ttaaaagttt aaaggaaatg ccgtctgaac caggtttcag
    14521 acggcatttg acgttcaggc agcttgcaaa cttatttcac ttccaacttg gcaacgccgc
    14581 ccatatacgg ttgcagtgcg gcagggatgt tgatgctgcc gtcggcgttt tgatggtttt
    14641 ccaatacggc gaccagcgtt ctgccgacag ccaagccgga gccgttcaaa gtatgtacca
    14701 agcggttttt gccgttttcg tctttgaaac gcgccttcat gcggcgggct tggaaatctt
    14761 cgcagttgga gcagcttgag atttcgcggt aggtgttttg cgccggtacc caaacttcca
    14821 agtcatacgt tttggttgcg ccgaagccca tgtcgccggt acacagggta atcacgcggt
    14881 agggcagttc caaagccttc aggatgtttt cggcatggcc gaccatttct tccagcgttt
    14941 cgtatgattt ttcgggatga acgatttgaa ccatttccac tttgtcgaac tggtgctggc
    15001 gaatcagacc gcgcgtgtct ttgccgtacg aacccgcctc gctgcggaaa cagggcgaat
    15061 gcgcggtcag cttcagcggc agttgttcgg acgggataat gctgccggca acggtattgg
    15121 tcagggtaac ttcagctgtc ggaatcagat attgcgtggt tttgctctcg tcgccgccac
    15181 gggtaacgtg gaacaaatct tctgcaaact tcggcagctg acccgtgcct tgcaaggtgg
    15241 tgtcgtccac gatatagggt gtgtaatgct cggtgtagcc gtgtttcagc gtgtgcgtat
    15301 cgagcatgaa ttgcgccagc gcgcggtgca ggcgggcgat ttgtccgcgc atcacggtaa
    15361 atcgtgcgcc ggaaagtttc gcgccacctt cgaaatccaa acccaaaggt tcgcccaaat
    15421 cgacatggtc tttgatttca aagtcaaatt cgcgtggcgt gccgactttg cggacttcga
    15481 cgttttcggt ttcatcctta ccgaaaggca cgtcctcatg cggcaggttc ggaatgctca
    15541 acagccatgc gtccagttcc ttttgcacgg cttccaaatc ggcggctgcc tgttccaaat
    15601 cggttttgat ttgggcgact tgatccatgg ccgcctgcgc ctcttcatgt ttgccctgac
    15661 ctttcagtgc gccgatttgt ttggaaatgc tgttgcgcga ggcttgcagc tcttcggttt
    15721 tcacctggac ggcttttcgc tgctcttcca gcgcattgaa acgcgcggca tcaaacttgt
    15781 aaccgcgtgc cgccaagcgt tccgccacag cggcggtgtt gctgcggagc aattggatat
    15841 ctaacatggt ctgattcccg aattaatttg aaacgtatta ttgtaaacca aatttaagat
    15901 attttcccga atccgtctgt gtttcgacat gcctgaaccc tatcgtccga tttgtcaaaa
    15961 ccgtccgatt tgtgggaaaa tcccaagatt gaatttaacg gagggcgaca ccgtgttgga
    16021 cagggaaggc tatcgcccca atgtcggtat catcttaatc aacaaccgcg acgaagtctt
    16081 ttggggcaag cgcgtgcgcg aacattcttg gcagtttccg caaggcggca tcaagccggg
    16141 cgaaagcccc gaaaccgcca tgtaccgcga gttgtacgaa gaagtcgggc ttttgccgca
    16201 acacgtcaaa attatcggcc gcacgcgcga ctggctgcgt tatgacgtgc cgaacaactg
    16261 ggtacgccgc gaatggcgcg gatcctatcg cgggcagaag cagatttggt atctcctgcg
    16321 cctgaccggc cgagattgcg atgtcaacct gcgcgccacc cgccaccccg aatttgacgg
    16381 ctggcgttgg catcaatatt gggcgcccgt tgacgaagtg attgatttca aacgcgacgt
    16441 ttatttgggg gcgttgaaag aactctcttc ccgcttcctg cgcggtatgg aaagttatga
    16501 agactttgcc gcccgccaat cttccgacaa ccgctaaaac aacggaacgc aacgctttca
    16561 ggccgtctga aagcgttgtt tttcttactt tcagattatt cctatggcta aaaacaacca
    16621 atacagcgaa tccagcatca ccgtcctcaa aggcttggag ccggtcaaag aacgtcccgg
    16681 catgtacacc cgcaccgaca gcccgaccca tatctgccaa gaagtcatcg acaacgcggc
    16741 ggacgaggcg ttgggcggtt tcgctacggc aatcgacgtg caaattcatg aagacggttc
    16801 gctttccgta cgcgacaacg gccgcggtat tcccgtcggt ctgcaccctg aagaaggcgt
    16861 acccgtagtc gaactcgtgt tcacccgtct gcacgcgggc ggcaagttca acaaaaaaga
    16921 cggcggcagc gcgtatgcct tttcaggcgg cctgcacggc gtgggcgtat ccgtgaccaa
    16981 cgccctttcc acccgccttg aggtaacggt caaacgcgaa ggcaaaatcc accgcatcgt
    17041 gtttgccggc ggcgacgtgg tcgaaccgtt ggcacaagtg ggcaaatgcg ccgtcaaaga
    17101 cagcggcacc gaagtgcgcg tctggccgga cggcaaatat tttgaaagcc cgaattacag
    17161 catccccgaa ctcgaacgcc tgctgcgcgc caaagccgtg ctgctgccgg gcgtgcgcgt
    17221 ttccctgacc cgtccggtca aaggcgaaga cgaagcgcac acccaaacct ggcattaccc
    17281 cgacggtctg aaaagctatc tgaccgacct gattgccgac gcgcaggaag ccgtgcctat
    17341 cttctcttgc gaaaactaca tttcagacgg ccacaacggc gatttcagca tcggcgaagg
    17401 cgccgcgttt gccctgacct ggctggaaga aggctcgtgc gccaacgaaa gctacgtcaa
    17461 cctcatcccc accccgctgg gcggcacgca cgaagcgggc ttgaaacaag ccgtgttcaa
    17521 cgccgtcagc aacttcatca atctgcacaa ccttttacca cgcggcgtga aagtgcaaag
    17581 cgacgacgtg ttcggcaaaa ccgcctttgt cctctctgcc cgtgtcctcg acccgcagtt
    17641 ccaaggtcag accaaagaca aactgaccaa ccgcgacgcg ctgaaactcg ttgctaccgt
    17701 atcgggcgac cctttggaat tgtggctgaa ccaaaacgtg gacttcggca aaaaaatcgc
    17761 cgaactcgcc atccgtcagg cacaggcgcg gatgcgttcg gttaaaaaga ttgaaaagaa
    17821 aaaaggcagc ggcgtcgccg tcctgcccgg taagctgacc gactgcgaaa gcgaagacat
    17881 ccgcgaaaac gaactcttcc tcgtcgaagg cgattccgcc ggcggctccg ccaaactcgc
    17941 ccgcgacaaa gccacccaag ccatcctgcc cctgcgcggc aaagtgctca acagctttga
    18001 agtccacccc gaccagctct tcggcaacgc cgaaatccac gacatttcag ttgccatcgg
    18061 cgtcgatccg cacggcatca acgaccatcc cgatttaagc ggcctgcgtt acggcaaaat
    18121 cgccatcctg tccgatgccg acgtggacgg ctcgcatatt caagttttgc tgctgaccct
    18181 gttctaccgc cacttcccga aactggtcgc tgacggacac atctacgtcg cccagccgcc
    18241 gctgttccgc gtagatgtca acgcacaagg taaaagcaaa cccgcccgca aattctacgc
    18301 cctcgaccaa aacgaacttg acggcatttt agagcggctg caaaaagaag gcgtcaaaga
    18361 aactgcttat tccatcagcc gtttcaaagg tttgggtgag atgaacccag accagctcaa
    18421 agatactacc atgcaccccg acacccgccg cctgttgcag gtacaaatcc ccgaaggcgc
    18481 ggatgacgag acgcgcgaca tcttcgtcaa actgatgggc aaaggcgaag ccgccgcccg
    18541 ccgtgcctgg atggagcgcg aaggcgatac ggcggaactc gatatttgaa ccgtgattgc
    18601 cgcatacgga aaaatgccgt ctgaaaccaa atcggttttc agacggcatt tcatttgggg
    18661 atttattctg ctgcggaaga ttcggctgct tcggcttcaa cagacggcgt ttcggcggca
    18721 gcttgggcgg actgctgttg caaagcctgc tgcacgaaag gatggttttg cgcgatcgcc
    18781 tgttcttcgg gggtaacttc gcctttgaaa cggttgttca aatcgaaacg tttgccgccg
    18841 cgcgccaagg ctttcagata gcgcggacgg cggcagtggt tggcgaggac gcgtgcaatc
    18901 agtgcggcat cgtattgcgg caaagcggca atcaaatcct gatcgatgcc gagtgccaac
    18961 ggtttgaagc gtttgaacac atcgtatttg ccgtagatgt ggtcggcgat catttcggtc
    19021 tgtttctttt tgctcatagt ttggacggcg gatttcagtg ccgcacccaa agcggtttct
    19081 tgtgtcattt cggttgtatt cctctaagaa tcgtgcgttc agacggcacg gcaatggtcg
    19141 gtattttagt ataaagcccg tgttttggct aaatcttttt acttgtccga gcgggcgata
    19201 tcgggcgtat ccacggcaac atccgcattt tgcgcacgat ggcgcagggc gtggtcgatt
    19261 aaaaccaagg cgagcatagc ttcggcaatc ggcgcggcgc gcaagccgac gcaggggtcg
    19321 tgcctgccgt gcgtggcgag ttcgatgggg ttgccgttga tgtcgatact gcggcgcggg
    19381 gtggcgatgg agctggtggg tttgatggcg atattgacgc agatgtcttg cccggtgctg
    19441 atgccgccga ggatgccgcc cgaatggttg gacaggaagc cttgcggggt cagttcgtcg
    19501 ccgtgttcgc tgccgcgttg cgttacgctg tcaaaacctg cgccgatttc cacgcctttt
    19561 acggcgttga tgcccatcat cgcgtaggcg atttcggcat cgaggcggtc gaaaacgggt
    19621 tcgcccaagc cgacggggac attggcggct tcaatatgca gcttcgcgcc gacggaatcc
    19681 aacgatttgc gcacgctgtc catatagttt tccagctcgg caatttggct atggttggcg
    19741 gcaaaaaaag gattttggga aatgtgttcg cagccttcaa accggatttc tttttcgccg
    19801 acttgggtaa cgtaggcggt gatttccgtg ccgaattttt ctttcaacca ttttttggca
    19861 acggctccgg cagcaacgcg ggcggcggtt tcacgggcgg agctcctgcc gccgccgcgg
    19921 tagtcgcgcg tgccgtattt gtgccaatag gtatagtcgg catggccggg gcggaaactt
    19981 tgggcgatgt tgccgtagtc tttgctgcgc tggtcggtat tgcggattaa gagggcgatg
    20041 ggcgtgccgg tggttttgcc tttgaatacg ccggagagga tttctacttg gtcggtttcg
    20101 cggcgttggg taacgtggcg gctggtgccg ggtttgcgtc ggtcgaggtc aaattggata
    20161 tccgcttcgc ttaattccaa gccgggcggg cagccgtcga tgatgcagcc caaacctgcg
    20221 ccgtggcttt cgccgaacgt ggtcacggtg aagagttgtc cgaaagtgtt gcctgccata
    20281 ttttccttct gtcggcggta tgttggagac ggcggatttt agcataaaag ttggaggaaa
    20341 acgggttata tgccggtaaa ggggttgcgt gttttgcagt gaatcggctt ataatccggt
    20401 ctgatttttc aggatagcga tatgagcgac tacacgcagc agcttcaaga taaaaaagac
    20461 caccttaaaa ccctttttgc aggtttggat gttcctgagt gggaagtgta cgaatctccg
    20521 gacaaacatt accgtatgcg tgccgagttc cgtatttggc acgaaggcgg tgaaatgttt
    20581 tatgccatgt ttgaaaaagg acaaaaggct ggcggggcaa gcctgatccg ttgcgaccgt
    20641 tttgaagcgg cttccgaagc cgtcaaccgc ctgatgccag aattgattgc cgttgccgcc
    20701 caatctgccg aactgagaaa ccgttggtat gccgtcgaat ttttgtctac gctcagcggg
    20761 gaaatgctgg ttaccatgat ttaccacaaa aggcttgata atgagtggat gcaggcggcg
    20821 caagcgttac agcaacagtt ggatatttcc gtcatcgggc ggagcagggg acagaaaata
    20881 gtcttaaaac aggactatgt aacggaaact ttgaaggtcg gcaaccggga tttccgttat
    20941 cggcaaattg aaggcagttt tacccaaccg aatgccgccg tgtgtcagaa aatgcttgaa
    21001 tgggcctgcg gtgcagcaga aggattggat ggggatttgc tcgaactgta ttgcggaaac
    21061 ggcaacttta cgcttccttt atctgaaaag tttgaacgtg ttttggcaac cgaaatctcc
    21121 aaaacctccg tcagcgcggc gcaatggaat atcgaagcca accgcatcgg caacattaaa
    21181 atcgcccgtc tgtctgctga agaatttacc gaagcctata ccggaaaacg tgagtttaaa
    21241 cgccttaagg atggcggtat tgctttgacg gattacgcat tttccactat tttcgtcgat
    21301 ccgccgcgtg ccggtatcga tgaggaaact ttgaagctgg tgtcgcagtt tgacaacatc
    21361 atctacattt cctgcaatcc cgaaacgctg cgtaccaatt tggacacctt ggcggaaacc
    21421 catgcggtcg aacgtgccgc cctgttcgat caatttccgt ttacgcacca tattgaaagc
    21481 ggtgtgctgt tgaaaaagaa aatccttgga aaaagcaaga ggtaaggact atgtaccgac
    21541 acatcgaata ctatcctggc gatccgattt tgagtttggt cgaaaccttc aaaaacgacc
    21601 cgcgccccga aaaagtcaat ttgagcatag gcatttattt cgacgacgaa ggcaaaatgc
    21661 ccgtgttgga atccgtgagc cgcgccgaaa ccgcccgcgc cgccactcct gtgccgtcgc
    21721 cctacctgcc gatggaaggc ttagacactt accgcagcgc ggtgcagcat ttgttgttcg
    21781 gcaaagacaa ccccgcgctc gcgcaaggac gtattgttac cgtacaaacc ttgggcggct
    21841 caggcgcgct caaagtcggg gcggactttc tgcaccgctg gtttcccgaa gcgcgcgcct
    21901 acgtcagcga cccgacttgg gacaaccatc gcggcatttt tgaaagcgca ggtttcgagg
    21961 tcggcactta cccatattac gaccctgctg ccgtcggcgt gaaattcgac gaaatgaccg
    22021 cgtttttcaa caccctgccc gaaaacagcg tcctgatcct ccatccctgc tgccacaacc
    22081 cgaccggcgt ggatatgtcg gaacaacaat gggacgaagt gttgcacatc atcaaaacgc
    22141 gcaaactgat tccgtttatg gacatcgcct accaaggctt cggcggcgat ttggacagcg
    22201 atgcctacgc cgtccgcaaa gcggtggaaa tggaattgcc cttgttcgtc agcaattcct
    22261 tctcaaaaaa cctgtcgctc tacggcgagc gcgtcggcgg cctgagcgtg gtttgcccga
    22321 ataaggaaga agcggatttg gtgttcggac agctcaaatt caccgtccgc cgcatctact
    22381 ccagcccgcc cgcgcacggc gcgtatatcg ccgccgacgt gatgaacagc cccgaacttt
    22441 acgccttgtg gcaaaacgag gtttatatga tgcgcgaccg catccgtgcg atgcggcaga
    22501 aactttatga tgtcttaact gcgcaaatcc ccgatcgcga tttcacttat ttcatcaaac
    22561 agcgcggcat gttcggctac acaggattga gcgtggaaca agtccgcagg ctgcgcgacg
    22621 aatttgccgt ttacctgctg gattccggca ggatgtgcgt cgccggactg aatacgtcga
    22681 ataccgacta tgttgcccgc gcatttgcgg aagtcttgaa ataagagttt gaagtaagtc
    22741 aaaagaccgt ctgaaaaata tttatctttc agacggtctc tttataattg gcgcacccaa
    22801 cagggatcga acctgtgacc tccagcttcg gaaactgaca ctcttccaac tgagctatgg
    22861 gtgcggaaaa cgtaaggtta acgtaaattc tgcaaattgg caaaggttat cttgcgccgg
    22921 caggcggcgt atggcggcat aaggagagtt ttcttgagga taaacaatta ttttcaaagg
    22981 attatttgtt ttatacagat accgttggca aattttttca tattttcaat gatagcgatt
    23041 ggaaagaccc tgtgaattca gtataatcca gcaaaatatt gttaactgcg tttaacacac
    23101 caaactataa tttacagcac aacctaacct gacggcgagg cctaccaaat gaaacaactc
    23161 cgcgacaata aagcccaagg ctctgcactg tttacccttg tgagcggtat cgttattgtt
    23221 attgcagtcc tttatttcct gattaagctg gcgggcagcg gctcgttcgg cgatgtcgat
    23281 gccactacgg aagcagcaac gcagacccgt atccagcctg tcggacaatt gacgatgggc
    23341 gacggcatcc ccgtcggcga acgccaaggc gaacagattt tcggcaaaat ctgtatccaa
    23401 tgccacgcgg cggacagcaa tgtgccgaac gctccgaaac tggaacacaa cggcgattgg
    23461 gcaccgcgta tcgcgcaagg cttcgatacc ttgttccaac acgcgctgaa cggctttaac
    23521 gccatgcctg ccaaaggcgg tgcggcagac ctgaccgatc aggaactcaa acgggcgatt
    23581 acttacatgg cgaacaaaag cggcggttct ttcccgaatc ctgatgaggc tgcgcctgcc
    23641 gacaatgccg cttcaggaac agcttctgct cctgccgata gtgcagctcc ggcagaagcg
    23701 aaggcagaag acaagggtgc ggcagcccct gcggtcggcg ttgacggtaa aaaagtcttc
    23761 gaagcaacct gtcaggtgtg ccacggaggt tcgattcccg gtattcccgg cataggcaaa
    23821 aaagacgatt gggcaccgcg tatcaaaaaa ggcaaagaaa ccttgcacaa acacgccctt
    23881 gaaggcttta acgcgatgcc ggccaaaggc ggcaatgcag gtttgagcga tgacgaagtc
    23941 aaagcggctg ttgactatat ggcaaaccaa tccggtgcaa aattctaaat ttagattaaa
    24001 atttcggatt aaataaaaac ggggcaggta tctgctccgt ttttttatgg gaatttatag
    24061 tggattaaca aaaatcagga taaggcgacg aagccgcaga cagtacagat agtacggaac
    24121 cgattcactt ggtgcttgag caccttagag aatcgttctc tttgagctaa ggagaggcaa
    24181 cgctgtactg gtttttgtta atccactata tttgtttgtc cgtgtggaaa tgacatcaaa
    24241 gggtgtgaat aggcttttca tctttgaaat ctgtttgcac gatcggatga ataaaaggta
    24301 ggatgataaa aaagctgatg tatggatact ggtttaactc atttgtttta aagttatagt
    24361 cgagatacgt gtattggtgc aaccagtctg ccgtcgacga aaatgccgtc tgaaagtgtt
    24421 tcagacggca tttgtcttcg gggtttcaac agtcagtctt cataattttc catcggtggg
    24481 cagctgcaca cgagattgcg gtcgccgtac acgtcgtcca cgcggttgac gaagggccaa
    24541 aacttgtgtt cgcgcacgaa gggcagcggg aagacggctt cttcgcgaga atacggatgc
    24601 gcccagttgc cggttatatc tgcggcggtg tgcggcgcgt tgaccagcgg attatcgtct
    24661 ttcggccatt cgccgtcgat gactttctgc acttcgcggc gaatggattt cagggcggcg
    24721 ataaagcggt cgagttcggc tttgctctcg ctttcggtcg gctcgatcat cagcgtaccg
    24781 gcaacgggga aggagacggt cggggcgtgg aagccgtagt ccatcaggcg tttggcgatg
    24841 tcggtttcgg taatgccgct ttcggctttg agcggacgca aatcgacgat gcattcgtgc
    24901 gcgacgcgtc cgtttttgcc tgtgtacaga atcggatagt cttcgctcag acgtttggca
    24961 acgtagttgg cgttgagcaa tgcccagcgt gtcgcctgct ccatgccttg tttgcccatc
    25021 atggtcaggt acatccaagt aatcggcagg atggacgcag aaccatatgc tgcggcagcc
    25081 acggcagttt gatcggcagc cgcgttgtgg gtgtcggtca aggtatggcc cggggcaaac
    25141 ggagccaaat gggctttcaa gccaatcgga cccatgcccg ggccgccgcc gccgtgaggg
    25201 atacagaagg ttttgtgcag gttcatgtgc aacacatccg caccgacttc ggcaggctgc
    25261 atgatgccga tttgggcgtt gaggttcgca ccgtccatgt aaacctgtcc gccgttttcg
    25321 tgaataatgc ggcagatgtc gcggatgcct tcttcgtaca cgccgtgggt ggacggatag
    25381 gtaatcatga tggcagacaa agcgtcgcgg tgttgctcgg ctttggcttt caaatcgtca
    25441 atgttgacgt tgccgtgttc gtcggtgtcg acgacgacga ctttcaaacc gagcatggcg
    25501 gcggtggcgg ggttggtacc gtgggctgat tttggaatca gacagatgtt gcggtgcgct
    25561 tcgccttggg attcctgata gcggcggatg gagagcatac cgctgtattc gccttgcgcg
    25621 ccggagttgg gctggaagga aatcgcgtca aagccggtga tggctttcag gctgttttcc
    25681 atatcggtca ggagttcgcg gtagccggcg gtttgcactt cgggagcgta aggatggatg
    25741 tcggagaact ccgcccaagt aatcggcagc atttcggcgg tggcgttgag tttcatggta
    25801 cagctgccca gcgaaatcat actgcggttc atcgccaagt cgcggtcttc gagttttttc
    25861 agatagcgca acatttcgtg ttcggtgtgg taactgttga acacaggatg ttgcagaatg
    25921 tcgtcttgac gcagcaattc ggcattcaga cggcctttga cgtcatcggc aaatgtgaac
    25981 gtatctttgc cggtaaacgc gcggtacaaa tcaaccaaat cttcgtatgc cgatgtttca
    26041 tggaatgcag ccgcaacttg agtatcgttg acgcggcgca gattgtaacc cgactccaaa
    26101 gcagcggcaa acacttggtc tgctttctct ttgctgccga aatcgaaggt aacggtatcg
    26161 aaaaagactt tgtgaaccac tttaatgccg tctgaaacca gcgcgtcggc aaaggcggaa
    26221 gccagcgcgt gaatgcgctt ggcaatgcgt ttcacacctt cggggccgtg gtaaacggca
    26281 tacataccca ccaagttcgc cagcaatgcc tgtgcggtac aaatattgga tgtcgctttt
    26341 tcgcggcgga tgtgttgctc gcgggtggac agagccatac gcagcgcggg tttgcccgat
    26401 gcatctttgg atacgccgat gatgcggccc ggagcggaac gtttgaactc gtctttaaac
    26461 gcaaaataag cggcgtgcgg cccgccgaag cccatcggta cgccgaagcg ttgagtgttg
    26521 cccaatgcga tgtccgcgcc caactcggca ggcgatttca gcaagaccaa gctcatgatg
    26581 tcggcggaaa cggccacgat tgtgcctttg gctttcagac ggccgataac gtcctgcaag
    26641 tcttgcacat cgccgtcttt gccgacgtat tggaacagcg cgccgaagta ttcgccttca
    26701 tccgctttgg caaaatcgcc gaccaccagc tcgaagccga aatacttggc gcgggttttc
    26761 atcacgtcca aagtttgcgg atacacgcgc tcgtccacaa agaaacgccc ggatttcacc
    26821 ttgcccacgc ggtgcgccat cgccattgct tcggcggcgg cggtcgcttc gtccaacaaa
    26881 gacgcgcccg ccacagggaa accggtcaaa tcgatacaca cttgttggaa gttcaacaaa
    26941 gcttccaaac gaccctgggc aatttccgcc tgatacggcg tgtaggcggt ataccagccc
    27001 ggattttcca atacgttgcg caaaatcacg ttcggcacgc gggtcgggta atagcccaag
    27061 ccgatatagg atttgttgat cacgtttttc gacgcaatgc ctttcaattt tgccaacgcg
    27121 tccgcttcgg tcaacgcttc gggcaaatcg agttcagacg gcatacggat gctttgcggc
    27181 acggtgttgc cgacaaagtc gtccatgctt tgctcgccga cagcggcaag caacgccgct
    27241 tcgtcgccga aactcaaatg ccgcgcggca aattcgttgg ggttgaacag ttcggacagt
    27301 ttcatttcta ctccttcggg tttgtagggc tgccatcagg cagaattttt agacatcgga
    27361 tgattcgggt aagggtagca caaaagacgg gataatggcg gacaatgcgc cagaatgcag
    27421 caggtcagga caagcagcgc cacaagcggc agcgataagg gaaagccgcc gaccaaaacc
    27481 atgaaaccct caagcaccag ccaagccaag ggatgcgccg gaagcggaaa aacaatattg
    27541 agcagaacca aaatcacgac aaacagcggc agcaccgcca tcaggttggc acgcctgtat
    27601 gttgcgtacg ggtcggatgc tttttcaggt tcgcaatatt tcatgatttc agtctgtccg
    27661 ggctttatcc gcagcaggtt cggacggtcg gtcatgccgt ccgaaccggc aggccgtctt
    27721 tttcaaaaaa cgccataccg ctgcaacttc atgcggctgt atggcgtagg ttttgcactt
    27781 cagacggcat atgcgtcccg aagcagtttg gtgttccagt cggttcatga ttatataaac
    27841 aacttgggtg cggataaacc tttgtttatc taaacccctg ttttctcaaa aattgattca
    27901 aatcttcggc aaatttccga tagcctttgc cgttggcgtg gatttggtcg gatttcagat
    27961 tattatcgcc caaaatttcc gcccacgcgc cgccgaacag cggaatgccg tattcctcgg
    28021 acaaatcctc atacagcgga tgatcgctca aatgcccgaa caacgcgccc agtgtgatgt
    28081 gcggcacgcc gacgaggacg gcggggatgt tttccttctg tacggtttcg atgattttcg
    28141 cgatattggc gcgggtctgc tccttgggaa ctttgcgcag aaagtcgttg ccgcctatgc
    28201 cgacaatcac aagcttgggt ttgcgtgcca acagcgcggg caggcgcgac agggcttggg
    28261 cggatgtatc gcccgatacg ccgccgttga caatattcca acccgtcagt ttttgcagtt
    28321 gcgcggggta ggattcgccg gggtttgcgc cgtagccgaa ggtgagcgaa tcgcccaagg
    28381 caagtacggt gcttccttcg ggaattttgg cgtgggctcg ggcggatttt ctgccgcagg
    28441 cggtaaggag caacgcgcct gcgccgagga ggaaggttct tctgttcatt ggtttttcag
    28501 atggcatcga gccatttcgg gtggtatttc agggtttcac ggtaaaccgg acagctttcg
    28561 gcgtgcgcgc ctttgaggta gccggtactc atcagaaatt cgccgacgat ttcgccgccg
    28621 acaaatttga aatgtttttt aaagagtttg acccattcgt ctttgcttcg cggatggtgc
    28681 gcgtcgagcc agttcttgaa cgagccgtat tcttgttgca acgcttggat ttgccgtgca
    28741 ttgaaaatgg cggcatcgat tttcaggcgg ttgcggacga tgcccgcgtc ggcaagcagc
    28801 cgctcaatat cggcttcgcc aaatgcggca accgtatcga tgtcgaaacc tttaaatgcc
    28861 gtctgaaatg cctgctgctt cttcagcatc agcgtccagt ttaatcctgc ctgattgatt
    28921 tccaacacca gccgttcaaa cagtccgttg tcgctgtcaa tcggaaaacc gtattgcgtg
    28981 tcgtggtaat gtttgttcgg gttatcggtg ttttcgggaa gtgaggcggc aaattcgcaa
    29041 tagttcacgg tttactcgac catctgttgc gccgcctgtt tcagtccctg ttcgagtgcg
    29101 gcggtcatcg cggcgtagcc gtcaccctgc tgttcggttt cgatatggaa gggtctgttc
    29161 gtaccgtcgg gtaggacggc gtagccgctg atgagggttt tgcccgtgta gctgccttgg
    29221 aatgcgtcga tatagaccgt ccatttttcg gtactgccgc tgcgtgaggc aggaacaaag
    29281 atgcgtgtgc tgtccaaacg gttgaatgca ttgctcaacg ccgcttcgag catatcgtcc
    29341 aaggtgtctg cccagacgtg gttttgtgcg gtgttgaggc ggtaggggtc ggtttgatag
    29401 accagtccgc cgcgtttgag cggttcggca agacggactt cgacggcagt ttcgccgcct
    29461 tgcgttgcag gacggatgta gcggctgtcg ggcaacacga aatattgtgt gctttgcaca
    29521 gtaccgcagg cggcaagcga cagggcggcg gcaatcggga aaaggcgcat tatcggcttc
    29581 ctttcgggat agggtctttg ctgctgctgt tgaaaatcag cgcgttgggt ttttctttca
    29641 aagtgttaat gacgggttga acgtctttta aggttttgtc caaactttgc agcgtatttt
    29701 gtacgtcgcc gtagataggc gattgcggcg atacgccttg cagggttatg cgcaactctt
    29761 tcagggtttg gttcagttcg ttcggaatgt tttgcgtctg cggtttgccg accagtttgt
    29821 cgatggagct tagggcggca ttggcagatt tgagtgtgga cttgagctcg gcaagcgagc
    29881 cgttcaattc ggcaaccgtc ttatccaaag gcagtttgcc gaatttgtcc agcaaatccg
    29941 ccaatttgac ctgtaagtcg tccaaaccgc cgccttgtgt agcgatgacg gtatcgcctg
    30001 cataaacggt atgcggtcgc agcttgggcg aagacgaagg ctgatcgttc aactcaatca
    30061 ttttgctgcc ggtcagcagg ttgttgctgg agatggtggc ggtcaggcct ttgtttaaag
    30121 ccgtctgaaa ttgttgtttc caatgttctt tgctttgctc gtcggcattg atttccaaac
    30181 gggaaggctc gatgcggatg cgtacgggaa tccaaccgtt ttcaaacaaa tgcaggctgt
    30241 cgttgcggtc gaaataaggg acatcggaaa ccacaccgac attcaggcct ttgtattcga
    30301 caggcgaacc ggcagacagg ccgcgtacgg attgtttgaa aaacgcggtg tagtatagcg
    30361 aacggtcgtc aggcaggttg gcgacttcgc tgcggctgtc gtaaagtgtg aagctgtctt
    30421 cgcttttaac gtttttgctg tcgcttgttt taggtgagtc aaaggaaatc gcgccggaca
    30481 gcagggcagg cagaggggcg gaattgagtt tgacaccgct gcctgtggtt tcgatattga
    30541 tgccgctttc cagccaaaaa cggctggcgg aatgaatcag tttgtcgttg gggctttgga
    30601 tgaagatggt gtaatgcacg ctttggtcgg acgggtcgaa atgcgcgctt tcgacttgcc
    30661 cgaccataaa gttttcatac aaaacagggc tgttgacgtt gaggatgcgg tcgtttttac
    30721 caatcaaatt caagcgcagc ccgctttgcc cgatggcggt aacgggcgga atgtcctgca
    30781 cttggaacac gtcttttgcc tcgtcgcttt tgccgggtgt aaaggcgatg tacgaacccg
    30841 aaagcagcgt acccaaaccg gttacgccgc tttggtcgat acgcggcttg accacccaaa
    30901 actgggtatc gctgcggatg aggccggata cgtccgcatt gagttgggcg gttacttcca
    30961 cgcctttttg gtcgtcgcgc agtttgattc gggtaacgcg tccgacatcg atgctcaata
    31021 ctttgatgac cgtattgttg acctcaatgc cttccgcgct gtccatcaag agcgtaacca
    31081 caggccccct gttgcggatt tccttaaccc aaagccagcc gccggcaatc agcgcaatca
    31141 gcgggaccag ccagacggca gagaggaagg tgttgttttt gcggacgcgt gcttgagcgt
    31201 gtccgtttgg aggagggctg ttgtcagtca tatttttccg tttcattgaa agcaatgccg
    31261 tctgaagcgc gtttgtccca aagcaggcgc gggtcgaaat aataggcgga cagcatcgtc
    31321 agaatcacga ccaggcagaa atagactgcc gcactgcccg gaatgacacg cgcggcataa
    31381 gtgtggaacg aacacatcaa aataataatc acaaaaatat caatcatcga ccagcggccg
    31441 accgcttcgg tgatgcggta gaggtgcgac aatttctttg cgcccgttgg caaagcgaag
    31501 cgggcggacg taatcaaaac cgacattgcc gcaatcttca gtaccggcac caaaatactc
    31561 gcgctgaaaa taaccgccgc aatcagcctg tcgccctcgt cccacatata agcgatgccg
    31621 ttaaggatgg tattgacctc tgtggcggca ggattggacg aaatcataat cggcaggata
    31681 ttggcaggga aatacaaaat aaccgccgcc gtcagaaacg ccgacgaaat actcagactt
    31741 ttcggccgtc ggcggtacag ttccgcgccg cacacgccgc agggggattc ggcactgtcg
    31801 cggaaataca ggcagcggct gcaacaggtt ttaccttccg atgccgtctg aaccgcatta
    31861 tcccccgtca gccgcccgat ttgaaaatac acccaatgct ggggaaccga taccgaagtc
    31921 cgaatcagca taaccgacag cgcgaacatc agataaaacg ccgatccgaa gcgaacctct
    31981 gccacagacg agagcttgat atacgccacc agagtggaaa caaaaaacac atccaccatc
    32041 atcgcctgcc tcaagcgcac catcacacgc gttgccaaac gcagcgcagg atacacccgt
    32101 ttccgtatca gcgcggcata gacatacagg cacagcagca gaaacagaac cggcgcgccg
    32161 aaggtcagca caaacatcac ttcggccaaa aaaccataat cctgaaacac catcaggcgt
    32221 accatttcgg gcagcgaaag gacggatgcc gcacccggta tcccgacctc gatatacgtc
    32281 ataccgtaag caaacgccat caaaatcagc gaagccgccg cataagcggg cggggcggaa
    32341 aaaggatgcc tgcccaccct gaagagtttg tgtccgcaac ggggacagaa cgccgcctct
    32401 cccttgtcca accgaggcac atccgcgcgg cagccgcatt ccggacaatc taccgtatgc
    32461 ggcggcaggg cttcgtcgcg gcagttgccg cgaaggctgt gggggatatt ttcggcaaac
    32521 ggcttcatcg ggaaagtgga aaatataagt ggcggcatta taaaggaaaa aagcaacgca
    32581 cccaatcagg atgcaaaacc ggtaggttcg gcagtatgaa caaacccttg tcgtccggca
    32641 aaaatatttt ttgagcggct gtccgccgac tgccgaaccc atttgataaa atcccgattc
    32701 tgtaaagaat agtaaaatac ctgcttaaaa acatcacgca aatgatattt tttccaaaaa
    32761 tatgtgataa taaatctcgt ttattatttt tgtatgcggt caggtacgga ttttgcccgc
    32821 actgcatcct taccccttac cgacaaagga aaagctatga gcgaaaccga aaatcaagcc
    32881 ctgacgtttg ccaaacgcct gaaggcggat actacggcgg ttcacgacag cgtggataac
    32941 ctcgttatgt ctgtccaacc gtttgtcagc aaagaaaact acatcaaatt tttgaaactc
    33001 caatccgttt tccacaaggc tgtcgaccac atctataaag atgccgaatt aaacaaagcc
    33061 attcccgagc tggaatacat ggcgcgatac gatgccgtaa cgcaagactt gaaagacttg
    33121 ggcgaagagc cttacaaatt cgacaaagaa ttgccacacg aaaccggcaa caaagcaatc
    33181 ggctggcttt attgcgccga aggttccaat ttgggcgcgg cgtttttgtt caaacacgcg
    33241 caaaaacttg attacaacgg cgaacacggc gcgcgccacc tcgctccgca tccggacggg
    33301 cgcggcaaac actggcgcgc tttcgtcgag catctgaacg ccttgaactt gactcctgaa
    33361 gccgaagcgg aagccatcca aggcgcgcgc gaagcctttg cattctacaa agtcgtgttg
    33421 cgcgaaacct tcggcttggc agccgatgcc gaagcccccg aaggtatgat gccgcacagg
    33481 cactaaaaaa taatcgaacc aaataaacaa ggtctcggca tagctgtttg cagggacctt
    33541 taattacacg gcgcggcttt gtttacatgg attactgtct tattaaatat taatgattat
    33601 cataaaatct attattcgct aaccgatgga tgaacaatcc atacatcttg agttgataat
    33661 atgaaaccat tacaaatgct ccctatcgcc gcgctggtcg gcagtatttt cggcaatccg
    33721 gtctttgcgg cagatgaagc tgcaactgaa accacacccg ttaaggcaga ggtaaaagca
    33781 gtgcgcgtta aaggccagcg caatgcgcct gcggctgtgg aacgcgtcaa ccttaaccgt
    33841 atcaaacaag aaatgatacg cgacaacaaa gacttggtgc gctattccac cgatgtcggc
    33901 ttgagcgaca gcggccgcca tcaaaaaggc tttgctgttc gcggcgtgga aggcaaccgt
    33961 gtcggcgtga gcatagacgg tgtaaacctg cctgattccg aagaaaactc gctgtacgcc
    34021 cgttatggca acttcaacag ctcgcgtttg tctatcgacc ccgaactcgt acgcaatatt
    34081 gaaatcgtga agggcgcaga ctctttcaat accggcagtg gtgcattggg cggcggtgtg
    34141 aattaccaaa cgctgcaagg ccgtgatttg ctgttggacg acaggcaatt cggcgtgatg
    34201 atgaaaaacg gttacagcac gcgtaaccgt gaatggacaa ataccctcgg tttcggcgtg
    34261 agcaacgacc gcgtggatgc tgctttgctg tattcgcaac gtcgcggtca tgaaaccgaa
    34321 agtgcgggaa accgaggcta tgctgtggaa ggggaaggca gtggcgcgaa tatccgtggt
    34381 tcggcacgcg gcatccccga cccgtccaaa cacaaatacc acagcttctt gggtaagatt
    34441 gcttatcaaa tcaatgacaa ccaccgcatc ggtgggtcgt tgaacggcca gcagggccac
    34501 aattacacgg ttgaagagtc ttacaacctg cttgcttctt attggcgtga agctgacgat
    34561 gtcaacagac gacgcaatgc caacctcttt tacgaatgga cgcctgattc cagctggttg
    34621 tctatggtga aagcggatgt cgattatcag aaaaccaaag tagcggcggt taacaacaaa
    34681 ggctcgttcc cgatggatta ttctacctgg acgcgcaact acaatcagaa ggatttggac
    34741 gaaatctaca accgcagtat ggatactacg ttcaaacgca ttacgctgcg tatggacagc
    34801 catccgttgc aactcggggg ggggagacac cgcctgtcgt tcaaaacttt cgccagccgc
    34861 cgtgattttg aaaacctaaa ccgcgacgat tattacttca gcggacagat atcccgtacc
    34921 accagctcga ttcaacaccc tgtgaaaacc accaactacg gtttctcgct gtctgaccaa
    34981 atccaatgga acgacgtgtt cagcagccgc gcaggtatcc gttacgacca caccaaaatg
    35041 acgcctcagg aattgaatgc cgagtgtcat gcttgtgaca aaacaccacc tgcagccaac
    35101 acttataaag gctggagcgg ttttgtcggc ttggcggcgc aactgaatca ggcttggcgt
    35161 gtcggttacg acattacttc cggctaccgt gtccccaatg cgtccgaagt gtatttcact
    35221 tacaaccacg gttcgggtaa ttggctgccc aatcccaacc tgaaagccga gcgcagcacc
    35281 acccacaccc tgtctctgca aggccgcagc gaaaaaggca tgctggatgc caacctgtat
    35341 caaagcaatt accgcaattt cctgtctgaa gagcagaagc tgaccaccag cggcactccc
    35401 ggctgtactg aggaaaatgc ttactacggt atatgcagcg acccctacaa agaaaaactg
    35461 gattggcaga tgaaaaatat cgacaaggcc agaatccgcg gtatcgagct gacaggccgt
    35521 ctgaatgtgg acaaagtagc gtcttttgtt cctgagggtt ggaaactgtt cggctcgctg
    35581 ggttatgcga aaagcaaact gtcgggcgac aacagcctgc tgtccacaca gccgctgaaa
    35641 gtgattgccg gtatcgacta tgaaagtccg agcgaaaaat ggggcgtatt ctcccgcctg
    35701 acctatctag gcgcgaaaaa ggtcaaagac gcgcaataca ccgtttatga aaacaagggc
    35761 tggggtacgc ctttgcagaa aaaggtaaaa gattacccgt ggctgaacaa gtcggcttat
    35821 gtgtttgata tgtacggctt ctacaaaccg gctaaaaacc tgactttgcg tgcaggcgtg
    35881 tacaacctgt tcaaccgcaa atacaccact tgggattccc tgcgcggttt atatagctac
    35941 agcaccacca atgcggtcga ccgcgatggc aaaggcttag accgctaccg cgccccaggc
    36001 cgcaattacg ccgtatcgct ggaatggaag ttttaatctg gtattattga attaatcgcc
    36061 ttgttgaaaa ttaaagccgt ccgaattgtg ttcaagaatt cattcggacg gctttttacc
    36121 gtaaatctgt gtgatggggt tttatagtgg attaacaaaa atcaggacaa ggcgacgaag
    36181 ccgcagacag tacagatagt acggaaccga ttcacttggt gcttcagcac cttagagaat
    36241 cgttctcttt gagctaaggc gaggcaacgc cgtactggtt tttgttaatc cactataaat
    36301 agcaaggtgt gaataaaacg gcggatataa tcaaatatat gtaatgagat tcccaaaatc
    36361 ttggtaatga tgtatattag tcaagttagc taatcatgag aagtgttatg tttcaagcaa
    36421 atattcatca ggcaaaaacc aatttgagtc aattgattca aagagcagaa gcaggggaaa
    36481 tcgttattat tgcgaaggca ggtaagcctt gcgtccaact aatcggtatt gaaaaaccgg
    36541 cacgaaatgc aggaaggttg aaaaaattca gtcatatgga aaatacggat atttcacgta
    36601 ttcttgagga tgacaatgaa acggcagctt tattttttga ggagtcggct ctgtgagaaa
    36661 gattctgctt gatacccatg cgttgctgtg gtggttgttg gatgacaaga aactggggat
    36721 atctgcacgc aaactgatag aaaatccgag aaatgcgatc tttgtcagtg cagcaagtat
    36781 ttgggaaatt tccatcaagc agaacaaggg gttgttgaaa ttaccggaag agttttttga
    36841 tgtgcttcaa gaagaggatt ttgaaatgtt gcctataggt ctgtttcatg caaaacaggc
    36901 tggaagcctt ccggagatac acaaagaccc tttcgatagg atgttgattg cacaaaccca
    36961 agcggaaggc tttgaactga tgactgtcga tgaatatatt ccgcagtatg gaattagagt
    37021 tgtcaacgca tcaagctgac aaatacaaaa actgtgtaca caagaaagac cgccgaggcg
    37081 gtcttttcgg ttattgtctt cgcttaaaag ccagacttcc gtcgcggcct ataaacgcgg
    37141 gaaatccgcg tgtataggca tagttggcga ccacaagtgc aaccttaagt gcatctccca
    37201 actttggcag ggtgtggtat gcacgcatgt attctatatc ctgtccatca aatttgggca
    37261 agacttcgct attgaaccgt ttgatcttcc tttcctaaaa aagcgcataa agagcaggta
    37321 cttaatatat atggctgtgt tttatagtgg attaacaaaa accagtacgg cgttgcctcg
    37381 ccttagctca aagagaacga ttctctaagg tgctgaagca ccaagtgaat cggttccgta
    37441 ctatctgtac tgtctgcgac ttcgtcgcct tgtcctgatt tttgttaatc cactataata
    37501 tagtgttgat agacgtattt aacttaaatg tattcaatag gataggtctc ttcctaaccg
    37561 aaaaaataat tgtcttttcc tttgcaaata attgaaatca acagattact aaaacacagc
    37621 ctaatttttt gattgccgat atgttaacgg acgacattgg cgcgcatgac cacgcggacg
    37681 tggaacgaac ctgtctgaca gtcgtattgc ccgccttgca gcggttgctt gtcgaagtag
    37741 ctgtggaagc cggtaacact gcgtccgccg agtttgccgg cggtctgctg gaatttgcgg
    37801 gcggcattga ttaaggcgcg ttcgcaggcc tcggagtcgg atttgccaac gccgtttgct
    37861 gcttgccgtg taaccagatt cctgccgttt gcaaccggta cgcccccgcc gccgaagctg
    37921 acgggaatcc cggattccaa tacgccgtct gcaatggctg catttattgc cgcctgaccg
    37981 ttgctgcaac ggtaaatgat gtttttgccg cttcctgcgg tgtcgccccg gatgactttg
    38041 catgattctc cggccggtgt gccggcggat gccgtgtgcg cgtttttagg tgcgcggctg
    38101 gattggcagg ctgccagcat cagggcggcg gcggagagta tgaggatttt tttcatgtcg
    38161 gatgtccttg cttgctgtgg gaaatcatta gcattgtaca gtaagattca acaaaatgcc
    38221 gtctgaaaac gggtttcaga cggcattgtg gctcaggggt tcaacacgcg tgcgatgagg
    38281 gctttttctt tgccctgcat atcggggatt ttgacctgaa tgccgttgcc gggtgcgaca
    38341 tcgacaggct ggcctttgcg ggtcatttgc tccaatttga tggtttggtt gccgctcggg
    38401 tggatgattt cgagcgaatc gccgacggcg aagcggtttt taacttccac tgttgcccag
    38461 ccgttttcgt cgatttcggt aacgtggccg acatattggc tttgtttggc ggtggaatgg
    38521 ccgctgaggt agttttgata atcctgagtt tggtggcgtt cgaggaagcc gctggtgtag
    38581 ccgcggttgg cgaggccttc gagttcgctc aacaggctgt aatcaaacgg acggcctgca
    38641 acggcatcgt caatcgcttt gcggtaggac tgggcgacgc gtgcaacata atagagcgat
    38701 ttggtgcggc cttcgacttt gaggctgtcc acgccgattt tggcgagttt ttcaacgact
    38761 tcgataccgc gcaggtcttt ggagttcatg atgtaggtgc cgtgttcgtc ttccataatc
    38821 ggcatcattt cgcccgggcg gttggactct tcaatcagga agactttgtc ggcatagggg
    38881 tggcgttttt gaccgttgat gccttcaaag ttttggttgg cttcttcttg ggctttttca
    38941 aagttaaaac cttgcagcag ttgggcatcg cccgcatcgc tttcagtagc gttgtggact
    39001 ttgtaatccc agcggcagga gttggtgcag gtgccttggt tggggtcgcg gtggttaaaa
    39061 tagcccgaca acaggcagcg gcctgagtaa gcgatgcaca atgcgccgtg gatgaagact
    39121 tcgagttcga tgtcggggca ttcttggcgg atttcagcaa tttcttccat actcaattcg
    39181 cgcgacagaa taatgcgttc gacgccgatg ttttgccaga atttcacgcc ccaatagttg
    39241 gtggtgttcg cctgtacgga cagatggatc ggcatttccg gccatttttc gcgcacggtc
    39301 ataatcaaac ccggatccgc cataatcagc gcgtcgggtt tcatcgcaat cagcggctcc
    39361 atatcggcga cgaaggtttt gagtttggaa ttgtgcggca gggtgttgac ggtcaaaaag
    39421 aattttttgt tgcgctcgtg cgcttcttta atgccttgct cgaggacgtc gagtttggca
    39481 aattcgttgt tgcgggcgcg cagggagtaa cgcgggctgc cggcataaac ggcatctgcg
    39541 ccgtagtcgt aggcggtgcg catcctttcc aatccgccgg cgggcaataa gagttcgggt
    39601 gctttcatcg tgtttccttt tcggttgaaa ccccgccctt tagggcggta gaatcagact
    39661 ttatttggga gggacgtaac cctttccgaa tcagggcaac atatagggcg acgctttatg
    39721 tgtcgtcctg tgtgttgaaa catgatttgt gtgctttgta aaaatgtttc agacggcatt
    39781 gctgcaaatg ccgtctgaag ggtgtttgaa gatgggcgga ttatccgcct tttcgtgttc
    39841 gcggtcaatt ttgccgggaa atttataagg atttatcaag tatttgcctg attgggatac
    39901 aatgcggcag tttgaatgcg agagagaatg tgatgaatcc taaaatcgtg tttttcgata
    39961 ttgacgatac gctgtaccga aaatatacgg ataccttgcg tccttccgtg aaaacggcgg
    40021 tggcggcttt gcgcggcaaa ggtatattga cggcgttggc gacggggcgg tctttggcga
    40081 cgatacccga aaaggtcagg gacatgatgg cggaaacggg tatggatgcc gtggtaacga
    40141 taaacgggca gtttgcgctg ctgcacggaa aaactgtgcg cgagatgccg atggatgtcg
    40201 ggcagatggg tcgggcttgt tcttttttag atggattggg gatggattat gctttcgtcg
    40261 gaggaagggg gattgtcgtg tccgcgctgt cggaatgcgt gtgccgcgcc ttgaagcata
    40321 tcgccagcga tttttttgcc gataaggatt atttctcaag caaaccggtg tatcagatgc
    40381 tggtgtttgc ggaggaaaac gaaatgccgc tctggtcgga tattgtggaa cgggaaggct
    40441 tgaaaacggt gcgctggcac gaggaagcgg tcgatctgct gccggccggc gcgtcaaaaa
    40501 cagacggtat cagaagcgtg gttgaagcat tgggtttgga aatggcagac gtgatggcgt
    40561 tcggcgatgg tttgaacgat gtggaaatgc tgtctgaagt cgggttcggt gtggcaatgg
    40621 gcaacgggga acaggcggcg aaagaagcgg cgaaatatgt ttgccacagc gttgatgaag
    40681 acggcgtgtt gaggggcttg caagatttgg gcgtgatttg aacgcatcat aacaaccctg
    40741 ccggtttcag acggcagggt cgattttcag cccttcatgc agccttcgtt ttgaagcagg
    40801 gtaaataagg gcgcgccgct tgcacggata ttcttgccgc cttgaaggtc ggtaaattcc
    40861 aaaatggcgg cagcttcgac aatttccccg ccgagtttgc ggatcagctc cagtccggca
    40921 agcatcgtgc cgcccgtggc aaccaaatca tcgaccagca gcacgcgcga accgggtttg
    40981 acggcatcgg tgtggatttc caccgcagct tccccgtatt cgagcgcgta gctttgcgat
    41041 acggtttcaa aaggcagctt gccttttttg cggatgggga cgaaaccgac attgagctgg
    41101 taggcgagtg ccgcgccgat aatgaagccg cgtgcgtcca aaccggcaac gatgtcgatt
    41161 ttctgatcca tatagcggta aaccaataaa tcgaccaaaa ggcggaagta ttccgcgctt
    41221 tgcaggacgg gcgtgatgtc gtggaataag atgccttttt gcggccagtt ttcgattttg
    41281 cggattttgt cggcaagcgc gccgacactc atagcttcgg gatgaaccag cattgcgtgt
    41341 tccaagtttg atgtttttaa cgccatattg tagccgtcag cctgccgctt gccaaatcaa
    41401 tcaaaacaat agcggcaaaa tatgggcatc ggcaaatagg aaatcgggtg ttcgttgtga
    41461 tacaatcggc tggaaaatat acaggtaaaa aatatgtccg cttatcgaaa aggcaacatc
    41521 tttatcattt cggccgcttc cggcacgggc aaaaccacgc tggtgtcgcg gctgttggca
    41581 aatcataacg gtttgcgcgt ttccgtatcg cacacgacgc gtccgccgcg tgaaggcgaa
    41641 gtaaacggcg tgcattatca ctttgtttcc aaagaagagt ttgagtcgct tatcgcgcag
    41701 gaagcttttt tggaatacgc agacgtattc ggcaactatt acggcacaag cgcggagggc
    41761 gtgaatgcgt tggcggcggc aggctatgac gtaattttgg aaatcgacgt tcagggcgcg
    41821 gcgcaggttc gagatgcgct gcctgaagct gtcggcatct ttatcctgcc gccgtctttc
    41881 gatgtgcttg ccgcgcgtct caacggacgc gggacggaca gtcgggaagt tatccaaagg
    41941 aggcttgcga aggcaaggca tgaaatcgaa cagtccgtat tgttcgactt tgtcgtggtc
    42001 aatgacgact tggcgcgggc ggaggaggat ttggttcata ttgtgaatgc ctgccgtctg
    42061 aaaaggtcgc ggcaactggg atttattgca gatttgttgg aaaattccta aaaaaccgcg
    42121 aaaatactcg gtttcccaat ttaaatattt ttgaaagaaa gcaaataata tggcacgtat
    42181 taccaccgaa gactgtaccg gaaaaatttc caaccatttt gacctgacat tggtagcggc
    42241 ccgccgcgcc cgccagcttg agaacggcaa cacgccgctt gtggacgatg tccgcaataa
    42301 caaaccgacc gttaccgcct taagggaaat cgccgccgga catatcggta cagaactgtt
    42361 gacgcgcaat aaataaattc tgccggaaac gcacgtcggc acattctgcc gccgtgcagt
    42421 ccgacggttt aaaatgaaaa acacatacat accgaaagcc gtctgaaatg cccgcccccc
    42481 aaccttccgc cccttacgac cccctgaccg ccgaagcgcg tgccctgctt ttccataccg
    42541 cctcctacct caagcccgag gaacaggcgg agcttgaaaa agctgtcgcc tatgcgtttc
    42601 gcgcccacga cgggcaaacc cgcaaaagcg gggagcccta catcacgcat ccgattgccg
    42661 ttgcgacgca gctcgccctt tggcatatgg acatacaggg tctttgtgca ggcgtgatgc
    42721 acgacgtatt ggaagatacg ggcgtgacaa aaggggaaat ggcggcggtg ttcggcaata
    42781 cgattgccga gatggtggac ggtctgtcca agcttgaaaa actcaaattt gaagatcatg
    42841 cagagcatca ggcggagagt ttccgcaaac tgattttggc gatgaccaaa gatgtgcgcg
    42901 tcatcgtcgt caaacttgcc gaccgcctgc acaatatgcg gacgctcggt tcgatgcgcc
    42961 cggacaaacg ccgccggatt gcaagggaaa cccttgaaat ctatgcacag attgccaacc
    43021 gtataggttt gaataacgca tatcaagagc ttcaggattt atcgttccaa aacctgcatc
    43081 ccaaccgcta cgagacttta aaaaaagcga tggacaagag ccggaagaac cggcaggacg
    43141 ttgtcggcaa agtcttgcgc gcattcggcc agcggctggt aggcgcgaat atagaggcca
    43201 aaatcaaagg cagggaaaaa aacctgtacg gcatccatca gaaaatgatg gcgaaaaagc
    43261 tgcgctttgc cgaggttatg gatatttacg gtttccgcgt cattgtcaac agcattccag
    43321 cctgttatgc cgcactcggc gcattgcaca ccctctatca gcccaagccc gggcggttca
    43381 aagactatat cgccattccg aaaagcaacg ggtatcaaag tctgcatacg actttggtcg
    43441 gtccttacgg cttgccgatt gaagttcaga tacgtaccaa ggaaatggat gctgttgccg
    43501 aaggtggaat cgccggacat tggagctata aatcatattc taagacggtc gatcaggcgg
    43561 tgcttcacac aaaccggtgg ctgaaaaata tcttagattt gcaggcaagc agtgccaatg
    43621 ccattgagtt tctcgaacac gtcaaagtcg atttgtttcc gaacgaaatc tacatcctta
    43681 cgccaaaagg aaaaatccta actttgccca aaggggcaac gcttgtcgat tttgcttatg
    43741 cggtgcatac cgatatcggg cacaaaaccg ttgccgcacg tatcaacaat atcatgatgc
    43801 cgttgcgtac gaagctcaaa acaggcgatt ccgtcgaaat tattacatcc gaacatgcca
    43861 aacccaatcc tgcatggttg aatttcgccg tgtcaggcag ggcgcgcagt gccatacgcc
    43921 aatatattaa aaaccttaac cggcacgatg cggtcgtttt gggagagagc ctcttacaaa
    43981 aagccctgtc cagtttgctg cccaaagatg tcctgctttc agacggcatc aaagaaaaat
    44041 accttgccga tctcaacgac aaacagactt catttgagga agtgctgtac aacgtcggta
    44101 tggggcatac cctgcctgtt tatgtcgcta tgcacattgc cgagttggca ggggagcatt
    44161 tcggcagcga ggtcaggctc agttccatta aagttgatgg tcaggaaagc gggcatattc
    44221 attttgcaga gtgctgccac cctgttcctg gtgattccat ccgtttgctg ttggttaagg
    44281 gaaaaggcat gattatccac agagatacct gcccgacgtt gttgaagtcc gatcccgaac
    44341 agcagctgga tgcagactgg gaaaatatga acgggcagaa ctaccgtgtc gggcttcaag
    44401 tccaatcgga agacagccac ggcctgttgg cattaatggc gcaagcgatt tccgattccg
    44461 gtgcggacat tgagtcggtc gaaacaccgt ctaaatccca gtcgggaacg gaaggttttg
    44521 tcgaattcaa attcttattg aaagtcaaga atctgaatca attgaatcag attattcaaa
    44581 atctgcatag tattccatat atccgcaaag tcatcagaag ttgaagcagg taaagcgggt
    44641 tgccttttga ttaacaggca acccgcttta tggtttatcc gttcaattgc ttatttgttc
    44701 aaatatacgg caagcctttc aacaatctgc atcgccgctt catctttact tgtttcggga
    44761 aaagatagtt ccccgtcgtc cccgataatg gtaatccggt tggtcggttt gcccatcgcg
    44821 attgaaacat cattggcaac tattaccggt atttttttct taatacgttt ttctcgtgca
    44881 tatgccatta cattctccgt ttcagcggca aaaccgatgc agaacggcgg gttcggcaat
    44941 gaggcaatag aagccaaaat atcggggttc tcatccaatt cgatggatag ccgtttggca
    45001 tttttatctt ttttgaattt ttgcgtactc ctgtttttaa ccttatagtc tgagacggcg
    45061 gcaacagaaa taaaagcatc ttgtttgtcg attaaacgat gcactgcgcg atgcatattt
    45121 tcggcactga cggcttgaac cgtatcggat atgccgaaag gcagcgcggt ttgaagctgt
    45181 ccgtgaatca ggctgacttc cgcaccggcg gcacggcacg cccgcgccaa agccacgccc
    45241 attttcccgc tggagatatt tgtgatgcct cggacagggt caatggcttc aaatgtcgca
    45301 cctgcggtaa tcaagacttt tttgcccttt aaaattttcg gtgtccataa atccggaagc
    45361 agatccagca attcggcagg ttccggcatc cttcccatac cattttctcc gcaagcctgt
    45421 tcgcccaagc ccggcatata gacagtaatg ccgtctgaaa ccagttgtgc gatattccgt
    45481 tggttggcag gattcagcca catttccaca ttcatcgcgg gagcgatggc aagaggacat
    45541 ttccgtgcgg ctgccagatt ggtcagtagg ttatctgcca cgccgttaca gatttttgcc
    45601 acggtattca tacttgccgg cgcaatcaga aaaacatccg cattccgggt caggttgata
    45661 tgttccatac cgtttgaacc gttgccgccg tgcgtgtcgg tcaggacagg attgccgctt
    45721 aaagcctgaa aagtcagcgg agaaacaaat tcagttgccg agcggctcat aaccaccgta
    45781 accgaatgcc cctgtttttt cagcagccga accaactcgc aagacttata cgccgcaata
    45841 ctgcccgtta cacctaaaag aatatgtttg cccattttga caatttctaa ttacgaagaa
    45901 aaaacagcag ccattctata acaaagtacg gatacattag ttaccgtttt ggatacaaaa
    45961 tgccgtctga agccccaagt ttcagacggc atattcacaa aggcgcacca gtcagaggag
    46021 ggggaggaa
//
LOCUS       DAWFIE010000005        43343 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWFIE010000005 DAWFIE010000000
VERSION     DAWFIE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 43343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.005"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(226..2001)
                     /gene="ptsP"
                     /locus_tag="WGQ71_000248"
     CDS             complement(226..2001)
                     /gene="ptsP"
                     /locus_tag="WGQ71_000248"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ0706325.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIAKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(2001..2270)
                     /locus_tag="WGQ71_000249"
     CDS             complement(2001..2270)
                     /locus_tag="WGQ71_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ0706326.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFQSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(2339..2776)
                     /locus_tag="WGQ71_000250"
     CDS             complement(2339..2776)
                     /locus_tag="WGQ71_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ0706327.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(2857..3420)
                     /locus_tag="WGQ71_000251"
     CDS             complement(2857..3420)
                     /locus_tag="WGQ71_000251"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ0706328.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(3489..4313)
                     /locus_tag="WGQ71_000252"
     CDS             complement(3489..4313)
                     /locus_tag="WGQ71_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ0706329.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQRYAFAPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRYGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTQKGTPKFATFVRARTDR"
     gene            4405..5037
                     /locus_tag="WGQ71_000253"
     CDS             4405..5037
                     /locus_tag="WGQ71_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ0706330.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(5243..7027)
                     /gene="rmuC"
                     /locus_tag="WGQ71_000254"
     CDS             complement(5243..7027)
                     /gene="rmuC"
                     /locus_tag="WGQ71_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ0706331.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAHLAEKAA
                     RCDFVEQAHGKTVSELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQHDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERSNETTALSESLEYVAEDEAV"
     gene            complement(7366..8154)
                     /locus_tag="WGQ71_000255"
     CDS             complement(7366..8154)
                     /locus_tag="WGQ71_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ0706332.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(8169..9518)
                     /locus_tag="WGQ71_000256"
     CDS             complement(8169..9518)
                     /locus_tag="WGQ71_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ0706333.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCSVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(9537..10118)
                     /gene="petA"
                     /locus_tag="WGQ71_000257"
     CDS             complement(9537..10118)
                     /gene="petA"
                     /locus_tag="WGQ71_000257"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ0706334.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(10241..10990)
                     /locus_tag="WGQ71_000258"
     CDS             complement(10241..10990)
                     /locus_tag="WGQ71_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ0706335.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            11121..12050
                     /locus_tag="WGQ71_000259"
     CDS             11121..12050
                     /locus_tag="WGQ71_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0706336.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAEWDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELESV"
     gene            complement(12305..12697)
                     /gene="rpsI"
                     /locus_tag="WGQ71_000260"
     CDS             complement(12305..12697)
                     /gene="rpsI"
                     /locus_tag="WGQ71_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ0706337.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12707..13138)
                     /gene="rplM"
                     /locus_tag="WGQ71_000261"
     CDS             complement(12707..13138)
                     /gene="rplM"
                     /locus_tag="WGQ71_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ0706338.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(13316..13495)
                     /gene="ssrS"
                     /locus_tag="WGQ71_000262"
     ncRNA           complement(13316..13495)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WGQ71_000262"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13501..13806)
                     /locus_tag="WGQ71_000263"
     CDS             complement(13501..13806)
                     /locus_tag="WGQ71_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ0706339.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(13817..14146)
                     /locus_tag="WGQ71_000264"
     CDS             complement(13817..14146)
                     /locus_tag="WGQ71_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706340.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(14201..15190)
                     /locus_tag="WGQ71_000265"
     CDS             complement(14201..15190)
                     /locus_tag="WGQ71_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ0706341.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMKEERE
                     NKHGLPGFPFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(15313..18015)
                     /gene="ppc"
                     /locus_tag="WGQ71_000266"
     CDS             complement(15313..18015)
                     /gene="ppc"
                     /locus_tag="WGQ71_000266"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ0706342.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVDVPDILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNRLNEEQKQTALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKETGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKIIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            18058..18951
                     /locus_tag="WGQ71_000267"
     CDS             18058..18951
                     /locus_tag="WGQ71_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ0706343.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDELLLRYSRHILLDEIGIEGQQKLSDAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALAGRLKRINHTVNVRAVNEKLDGCRL
                     TGLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAER"
     gene            complement(19366..19590)
                     /locus_tag="WGQ71_000268"
     CDS             complement(19366..19590)
                     /locus_tag="WGQ71_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ0706344.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(19591..20979)
                     /locus_tag="WGQ71_000269"
     CDS             complement(19591..20979)
                     /locus_tag="WGQ71_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ0706345.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTIGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGMMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(21065..21886)
                     /gene="prmC"
                     /locus_tag="WGQ71_000270"
     CDS             complement(21065..21886)
                     /gene="prmC"
                     /locus_tag="WGQ71_000270"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0706346.1"
                     /translation="MTFDKWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQCRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARENAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(21876..22337)
                     /locus_tag="WGQ71_000271"
     CDS             complement(21876..22337)
                     /locus_tag="WGQ71_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706347.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAAFWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVATIGTFAGKNKERKAAPAADGIKNDV"
     gene            complement(22402..23886)
                     /gene="tldD"
                     /locus_tag="WGQ71_000272"
     CDS             complement(22402..23886)
                     /gene="tldD"
                     /locus_tag="WGQ71_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ0706348.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      24064..24167
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            24234..25457
                     /gene="cytX"
                     /locus_tag="WGQ71_000273"
     CDS             24234..25457
                     /gene="cytX"
                     /locus_tag="WGQ71_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ0706349.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYASATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGVSANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            25454..26554
                     /locus_tag="WGQ71_000274"
     CDS             25454..26554
                     /locus_tag="WGQ71_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0706350.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWHADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECVPEDLQAQYDWLIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHIFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            26575..27192
                     /gene="thiE"
                     /locus_tag="WGQ71_000275"
     CDS             26575..27192
                     /gene="thiE"
                     /locus_tag="WGQ71_000275"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ0706351.1"
                     /translation="MTFLPLKSPLKFYAVVPTADWVERMVEAGADTVQLRCKALHGDE
                     LKREIARCAAACRGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLEKLREYVKQAGSTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
     gene            27614..27808
                     /gene="thiS"
                     /locus_tag="WGQ71_000276"
     CDS             27614..27808
                     /gene="thiS"
                     /locus_tag="WGQ71_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ0706352.1"
                     /translation="MNIILNGEPAELHGTSVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
     gene            28021..28809
                     /locus_tag="WGQ71_000277"
     CDS             28021..28809
                     /locus_tag="WGQ71_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ0706353.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSIQTAQPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(29572..30396)
                     /locus_tag="WGQ71_000278"
     CDS             complement(29572..30396)
                     /locus_tag="WGQ71_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ0706354.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(30509..32287)
                     /locus_tag="WGQ71_000279"
     CDS             complement(30509..32287)
                     /locus_tag="WGQ71_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ0706355.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRAGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            complement(32284..32790)
                     /locus_tag="WGQ71_000280"
     CDS             complement(32284..32790)
                     /locus_tag="WGQ71_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ0706356.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            complement(32926..33001)
                     /locus_tag="WGQ71_000281"
     tRNA            complement(32926..33001)
                     /locus_tag="WGQ71_000281"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32966..32968),aa:His,seq:gtg)
     gene            complement(33029..33105)
                     /locus_tag="WGQ71_000282"
     tRNA            complement(33029..33105)
                     /locus_tag="WGQ71_000282"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33069..33071),aa:Arg,seq:tct)
     gene            complement(33130..33207)
                     /locus_tag="WGQ71_000283"
     tRNA            complement(33130..33207)
                     /locus_tag="WGQ71_000283"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33170..33172),aa:Pro,seq:tgg)
     gene            33430..34284
                     /gene="folD"
                     /locus_tag="WGQ71_000284"
     CDS             33430..34284
                     /gene="folD"
                     /locus_tag="WGQ71_000284"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ0706357.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQNNLHTPCLAVVLVGGD
                     PASAVYVRNKKTSCQKCGIKSLSYELPESTSQEELLALVDRLNVDSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            complement(34346..34927)
                     /locus_tag="WGQ71_000285"
     CDS             complement(34346..34927)
                     /locus_tag="WGQ71_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0706358.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            35207..36322
                     /gene="asd"
                     /locus_tag="WGQ71_000286"
     CDS             35207..36322
                     /gene="asd"
                     /locus_tag="WGQ71_000286"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ0706359.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQPLRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGTIHAQVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            complement(36550..37080)
                     /locus_tag="WGQ71_000287"
     CDS             complement(36550..37080)
                     /locus_tag="WGQ71_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0706360.1"
                     /translation="MQGFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLRTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(37091..37435)
                     /locus_tag="WGQ71_000288"
     CDS             complement(37091..37435)
                     /locus_tag="WGQ71_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706361.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKVNPIWALVWLLICMPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            complement(37422..38201)
                     /gene="xth"
                     /locus_tag="WGQ71_000289"
     CDS             complement(37422..38201)
                     /gene="xth"
                     /locus_tag="WGQ71_000289"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0706362.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            complement(38372..39793)
                     /locus_tag="WGQ71_000290"
     CDS             complement(38372..39793)
                     /locus_tag="WGQ71_000290"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ0706363.1"
                     /translation="MTTIYNTLTRQKEPFTPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGRGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNDAQL
                     AGRLKALGGIIGLLQRDPTEFLQGSAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            complement(40481..41530)
                     /locus_tag="WGQ71_000291"
     CDS             complement(40481..41530)
                     /locus_tag="WGQ71_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0706364.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDACMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            complement(41613..42767)
                     /gene="obgE"
                     /locus_tag="WGQ71_000292"
     CDS             complement(41613..42767)
                     /gene="obgE"
                     /locus_tag="WGQ71_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ0706365.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDEAVDPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQERTVTFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRLEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            complement(43060..43343)
                     /locus_tag="WGQ71_000293"
                     /pseudo
     CDS             complement(43060..43343)
                     /locus_tag="WGQ71_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         9615 a        12164 c        11291 g        10273 t
ORIGIN      
        1 agaacgtaaa atctaaagaa accgtttttc ccgacaagtt tctgtgccga caaacctaga
       61 ttcccgcctg cgcgggaatg acggatttta ggtttctgat tttggttttc tgtttttgag
      121 ggaatgaccg attttgggtt tctgtttcgg ttttctattt tgcaagaatg gcaaaatttc
      181 agattgcggg cattgttaag tatttctatt ttttacctgc cgtatttatt tccgcccctt
      241 gaagtcggct tcttcctcga cagacacgct gttcatctgt ttgatcagct tttccgactt
      301 ctcttcgtct tcgcagcgga tgactttcgc aatatcgctt tcgagctgtc cgacattgct
      361 gtgcagaatg atgtttttga cgggcaggat gttgttgggg ttcatggaaa aacggcgcag
      421 ccccataccc aataaaacgc gggtaaacgc ggtatcgccc gccatctcgc cgcatacgga
      481 tacgtctttg tccatgcggt tggcggtacg gatgacgtgt tgcagcattt tcagcacggc
      541 gggatggccg ggctggtaga ggtggctgac gctgtcgtcg ccgcgatcga cggacaagat
      601 gtattgaatc aggtcgttgg taccgacgga gatgaaatcg accagtttca aaatactgcc
      661 gacggtcagc gcggcagacg gaatttcaat catacagccg atgccgactt taccgaaggc
      721 atcgccgcgt tcggcaagct ggcgttgcgc ggtgtcgagg tggatgaggc actggcgcac
      781 ttcggatacg gaggtaatca tcggccacat catccgcacg gggccgtgta ccgccgcacg
      841 gaggatggcg cgcatctggg tgcggaacat gaccggttcg gcaaggcaca ggcggatgcc
      901 ggtcatgccc agcgcggggt tgaggctgcc gttgggcgtg ctgtttttcc cgaaccagcg
      961 ggggttttta tccacgccca aatcgactgt ccgtatcgtt acgcttttgc ccttcatttt
     1021 tttgacaatc gcgctgtaca cttcgtactg ctcgtcttca gacggcatcg tatcgcggtt
     1081 caggtaaaga aactcgctgc ggaacagccc gatgccgtct gcgccgaggt tgtgcagcgg
     1141 tttcacgtct tcggcggatt ctatattgcc cacaagctcg atgcagaccc cgtcagcggt
     1201 ggcggcggcg gtttttttga gcttgttcaa atcgcgtttg tggctgcggt attcgcgggc
     1261 acggcggcgg tattcgttca acaccgactc atccggcgcg ataatcaaca cgccgttgat
     1321 accgtccaca atgaccgttt cgccctcggt aatcagtttg cgcgcgttgt gcagcccgac
     1381 gacggacggg atgtccaagc tcctgcccaa aatcgccgta tgcccggtgg ggccgccggc
     1441 atcggtaacg aaggcggcaa tgcgctgctc tttaaacaaa accgtgtcgg cgggcgaaag
     1501 gtcgtttgca atcagaacgg tttcgtcaaa caggttgtcg gcaacttcca actcgttgcc
     1561 ctgcccgatc aggttgttgt ggatgcggcg gacgacttgc agcatatcct gcttgcgttc
     1621 gcgcaaatag gcatcgtcca tattgtcgaa ttgggcggcg agtttgtcgc tctgctgctt
     1681 caatgcccac tcggcgttga ttttttgttc ccttaaaata tcgacgggtt cgcgcgacaa
     1741 ggtaacatcg gtcaagagca tcaggtgcag cgagatgaac gcgcccaact cggtcggggc
     1801 gttttcggga atcgcgctgc ggagctgttc caactctttg cgcgtggctt tgacggcggc
     1861 atcgaaacgt tcggcttcgg catcggtgtc cgcctccgca acatcatact gcggcacttc
     1921 ctccgtaccg cgcgcaatca ggtgggcgca accgacggca atgcctttgc ccgccgccac
     1981 gccgtgcagc acgatactca ttattcgccc tcgccgaagt agccgttgat taagtcggtc
     2041 agggcgcgca tcgcttccgc ctcgtccgcg ccgtccgtct ccagctcgat gaccgtaccc
     2101 ttggcggcgg cgagcatcat cagccccatg atgcttttgc cgttgacgcg gctgccgttt
     2161 ttcgttaccc agacttcgct ttgaaactga gacgcggttt gggtgaattt gctggaggca
     2221 cgggcgtgaa gtccgagttt gttgatgatt tcgatggatt gtttgagcat ttcgattccc
     2281 gtgttatgta tatcggcagc agacgccgtt taaaatgttt tcctgccctg ccgcttcttc
     2341 agacggcatc gccgctgcgc cggcacacca aatcttcggg cgcggacgtg atggcgaaaa
     2401 tgccttttac agccgcctct ctgacacatt cggtaaaggc ggcaaggtct tccgccgccg
     2461 gcgaatattg gacggcctta accatcatcg gcgcgttcag tccggtcaaa atcgccgatt
     2521 tgttttcgcg cacgaggcgg cgggctgcat tgcagggggt cgcgccgaaa atatcggtca
     2581 taatcagcac gccgtcgttg tcgggaaatt cctgaagcgc ggcaatggcg ttgttgatga
     2641 tgtcgtcttg gtcttccgtc ggttgcacgc cgagtatgcg gacgttttca ggcagcccgc
     2701 ccgggaaaaa atgatgtgcc agcttgcggt aggcttcgcc tatggtttcg tgtgtgatga
     2761 ttaaaagccc tatcatatta tgcgtcctgt tcctcgttgt cctaccggcg tatgggcgcg
     2821 atgccgtctg aaaggccttc agacggcatc gcgtccttat tttccgtcca atgcgtaaat
     2881 ctcgcccaga ttgcgccagc agcccgccgc atccatgccg taaccgaaaa cataacggtt
     2941 cggcacatcc agtccgacat aatcggctcg gataggcttg ggtttgtcaa tcagcttgtt
     3001 ggcgaacacc gccgcacggc agcttgccgc acccatttcc aaaagtttgg cttgaatggc
     3061 ggacatcgta tgcccttcgt ccaaaatatc gtccagcacg acgacgtgcc tgccccggat
     3121 ttgttccgca tcgggcatac gcttccagtt gaacgcgccg ccctccagct tgtcgccgta
     3181 acgggaaacg tgaacataat caaaatctaa gggaaaacgc aacagcggca gcaactgccc
     3241 cgtaaacacc accgcgccgc ccatcacggg cagcagcagc ggatatttgc cgcccaaatc
     3301 acgcgtaatc tcgtccgcca ctttttgcag tgcggcacgg cattggcctt ggtcgaacaa
     3361 aagatcggcg ttttcaagca tcgcctgtgt ttcaaggcgt ttggtttcta aatcggtcat
     3421 atgtcggaat cggtcggtaa aaggaaaatt ataaaccaaa gtatcggctg ccgtctgaac
     3481 cgtcctgctc aacggtcggt acgcgcgcgc acaaacgtgg cgaatttcgg cgtgcctttc
     3541 tgcgtaaagc cacggtagcg gtaggtaatc agtgtgccga ttttgggcgg gttgtctcgg
     3601 tctttatctt tgaagccgct gccaatgcgg aattcgccgt accggttttt gcagccgacc
     3661 gcgcccaacc gtccggcgtt tcgccctttg ccctcatagt gccgcgttac cgtgcattcg
     3721 tcgtcgtatt ggcttttcag cttcaataat tggctgctcc tgccgccgct gtaacgggat
     3781 tcgggctgac gcagcatcac gccttcgccg ccctgcgctt cgatttgttt taaaaagtcc
     3841 atcgcgtgct gccggtcgcg cactttgatt tgcgggatga tggtaatcgg cgcgttcgga
     3901 tgcgttttca gccactgcgt tgcgactgcc aaacgttggt agagattgcc ctgcgccttg
     3961 ggtacatcga aaacgtgcag gcggatgccg cgccagtctg aagaaacaga acgcacggta
     4021 gcggaaatct gctcgaactg accacgtccg ctatacaatt cgccgtccaa aggataaggc
     4081 ggaaactgag cggtaaaacc tttgggcgga gcaaacgcat acctctgacg gcttatcagg
     4141 tgctttccgt cccaataggc gcgcacgccg tcgagtttct cgctcatcgc ccagccggca
     4201 atgtcctgcc ctttgtattc ctgcgccagc atcaaatccg ccgcgcctgc cgatgcaggg
     4261 atgaaaaccg ccgtaaaaat cggtatgatg ccgccgattg tcttcttaat catctgattc
     4321 ccccaatatc aaaacgggcg gcaaaccgcc ataaaacaaa cggcaaaccc gatgccgtct
     4381 gaaaaaccgt ttaggaacac gccgatgacc ctacgttacg aaatcttccc cgttaccccc
     4441 ttccgccaaa actgcaccct gatttgggac gacgaaagcg gcgaagccgt cctgaccgat
     4501 gtcggcggcg acgtgccgtt cctgctgcaa gcgttggcaa accgcaaact tacgctcacg
     4561 gcaatctggc tgacgcacgg ccatctcgat cacgcgggcg gcgtggtcga aatgttgaaa
     4621 acgcataaag tccctgtcct cgggccgcat ccggacgatg aattcctgct ccaatcgctg
     4681 ccgcaaacca ccgcgcaata cggatttccc gtctcgcccg cctttgcgcc gaaccgttgg
     4741 ctcgaagaag gcgaaacgct cacggtcgga cgctatgcct ttcaagtgct gcatattccg
     4801 ggccatacgc cgggacatat cgtcttttat tgtgccgagg cggaattgct gattgcgggc
     4861 gacgtgctgt tttacgaaac cataggcaga accgattttc cgcgcggcaa ccacgccgac
     4921 ttaatcaata atatccgcaa caaattattc acccttcccg aaaccgtgca agttgtcgcc
     4981 ggacacgggc gtatgacttc catcggacac gaaaagcggc acaatccgtt tttctaaccg
     5041 ccttccctac ggtcttcaga cggcatcatc tgcactgatg ccgtctgaaa cgcaaaaggc
     5101 tcagacaacc gccgccttgc cggtgtacct cgccggacaa ggctttggta agtacttcaa
     5161 accaacccca aatcagaacc ggtacagtta cgccgcgctt tcggcattcc cgcccctgct
     5221 gaaacaagct tttccgcaca agtcagactg cttcatcttc tgccacatat tccaaagatt
     5281 ccgacaacgc cgttgtttca ttggaacgct cgaccaaatc ccgttgaagt tgtttgcctg
     5341 ccttcacgcc caacagacgc agtttctcgg cgcgtccgac cagattcccg cgcccttcgg
     5401 caagttgctt gaatgccgtc tgaaaactgc tttgcgcctg atcgatgcct ttgccgacgc
     5461 tttcgagcgt ctgtacgaag ccgacaaact tgtcgtacag cttgccgcct tcgtccgcaa
     5521 tcgccagtgc gttctgattt tgctgttcgt tgcgccaaat attcgccacc gtcctcaaag
     5581 tcgccagcag cgtactgggg ccgaccagca taatccgttt gtcgaaacac tcttggaaca
     5641 atcccgcgtc atgctgcaac gccagcaggt aggccggttc gacagggata aacataaaga
     5701 cgaaatccaa tgtgttcaca ccttccaaat cggtgtaatc cttcagcgac aagcctttca
     5761 tgtgtgcacg gatgctggca acgtgtgccg ccagttcgcg tgccgccgta tccgcatccg
     5821 ccgcctgcgt gtagcgcaca taagctgtca gcgagacctt ggagtcaatc acaatctgct
     5881 tgttgtcggg caggttgacc aaaacgtcgg gctggaggcg gcgcgtgccg ccgtcttcct
     5941 cttttcggac ggatgccgcc tgaaccacat attcgcgccc tttctggagg ccggaatttt
     6001 ccaaaaccgt ttccagaatc atttcgcccc aattgccctg aaccttattc tgcgtaccgg
     6061 tcagcgcgtt ggtcagtgcc tttgcctcgc tgtgcagctg cgcattcaat ccttgaagcc
     6121 gtttcaattc gttttccaac gtcagccgct cgcgcgattc tttatcatag gtttgcttga
     6181 ccaactcgcc gaaaccgtgg atgcgttcgt ttagcgggtt caaaacctga tggagctgct
     6241 cgcggttctg ctcggtaaaa cggcggcttt tttcttccaa aatcgtgttg gcaagatttt
     6301 gaaactgatc gctcaaactt ttgcgcgcct cgcccagtaa ggacagcttc tcttcagaag
     6361 caaggcgttc ctgttcgatt tgcgttgcca aacgttcgtt ttcaaccgcc aaaccctgtg
     6421 ccttttcctg caactcggta tgcgactgcc tcaaccgctc cgcttccgcc tctttttcct
     6481 gcaaatgggc aatctgtttt tcggctgcgg caaaacggtt gcccacatcg gcaaggtcgt
     6541 tttgcacgtc gcggacagtt tggcggcttt cttccaaatc ggtttcgatt ctttggcgga
     6601 tttggcgttc caaggcatat tggtcggcaa acgccttccg ttcctgtccg agctgcgttg
     6661 ccaaacgttc gttttcaacc gccaaaccct gtgccttttc ctgcaactcg atatacgact
     6721 gcttcagccg cgccgactcc gcctcttttt cctgcaaatg ggcaatctgc ttttcggctg
     6781 cggaaaaacg gttgcccaaa gcataatttt cgtcctgcaa atgccggtat ttcccgtcca
     6841 acaccgccaa ttccgacacg gttttgccgt gtgcctgttc gacaaaatca catcttgccg
     6901 ccttttccgc caggtgcgcg ttcaaaccgg caaactcgcc ctgaaacctg cccttcatca
     6961 gcaaccatgt aaacaacacg cccgacacca acgccgccaa aggcagcaaa acagtcatca
     7021 gttccatcaa tcatcctaat attcaaacat tttcacaccg gacaaaaccg ccgcttattc
     7081 cgattctacc tgtttgttcg acatagctcc aaaaaatata gcggattggc tttaaacctg
     7141 ttcgacatcg ccttaccatg ctgcttgcgg tttcagacct tttcctaatt caatatcaat
     7201 ctgccacaaa ccctgattaa gttcccgatg tctgacattt ttagaatgat gccgtctgaa
     7261 atgttgcagc tatgttcaga cggcatacgg attcaggctt ttcaaacggc aggcaaaatg
     7321 aaaaaagggc aaaccctaaa ggatttgccc ttttgttcca aacgcttagt gtacgtcttt
     7381 ccaatattct tttttcagga aatacgccaa aggcagcata accgcaaata ggaaaatcat
     7441 cacgacatag cctatacgtt tgcgttgcag ttgtgcaggt tcgcccatgt acacaaggta
     7501 attgaccaaa tcgcgtacat atgcgtcgta ctctttttgg atcactttgc cgttaggcag
     7561 acggcggctg tgcaaaccgg tagattccca atacagctta ggcttcatct cgccgtgttc
     7621 gtcttttacc ataaccggct gacctttggc atccaactca acggcttgaa cgccttgctg
     7681 ttcccacaac gggtggggca taccgacttt atcgaataca gtattgttcc agccgctcgg
     7741 acgggtcgga tctttataga agccgcgcat ataggcgtaa aggtagtctg cacctttgga
     7801 acgcgcaatc aacgtcaaat cgggcggagc agcaccaaac cattttgccg catctttcgg
     7861 gttcatcgcc gaatgcatga catcgccgac attatcggtg gtaaacatca ggtttttctt
     7921 gatttcttcg tcagtcaaac cgatgtcttt caaacggttg aaacgcatac cgcttgccga
     7981 gtggcaggac aggcaatagt tggtaaagat ttgtgcgccg cgctgcaggc tgacttggtc
     8041 gcgcaaatcg atatcgactt tctcatagtg tgcatgaccg cccgaagcaa atgccgcacc
     8101 cataggtact gccaacagca aggcagcaaa ccaatttttc atagcttgtt tcattttgac
     8161 tgccctcatc agatgttggt tgcaaacagg taagcaccca cggcggtaat gccgacgtaa
     8221 acaaagaaca tgattttttg tttggcggtg ctcatggtta cgcgttcggg aaccggtttg
     8281 tttttatcca gcttggtata gaacggcata cccaggaaga atgcgaaata gacgaaggac
     8341 aggatgcgcg cgaccaaagt acgcgtatcg gttgccacca tcgcgcccaa aatacccaaa
     8401 ccgataaagg aaatgatgaa caagaccaat gcggttttga aaatcgggcc gcggtaacgg
     8461 acggatttca cctcacccct atccagccaa ggcagcaggg caatcaacac gaccgccgcg
     8521 cccataccga ttacacccca aacctgcgtt cccaagaacg agggaatcgc acgcaaaatg
     8581 gcgtagaacg gtgtgaaata ccataccggc gcaatgtgcg gaggtgtttt cagcgcattt
     8641 gccgcatcga agttcggcgc ttcgaggaag tagccgccac cttctggtgc aaagaacaac
     8701 acggaacaga agacaatcag gaatacaacg acacccaaga tgtccttaac ggtgtagtaa
     8761 ggatggaatg ggataccgtc gcgcgggata ccgttttcgt ctttgttttt cttgatttca
     8821 acgccgtcag ggttgttaga acccacttcg tgcagggcga tgatgtgcgc cacaaccaag
     8881 ccgagcaata ccagaggtac cgcgataacg tgcagggcaa agaagcgatt cagggtaacg
     8941 tcggaaacgt tgaagtcgcc gcgaatccaa gtggacaaat ccggaccgat aacagggatg
     9001 gcggagaaca ggttaataat tacctgcgca ccccaaaagg acatctgacc ccaaggcagc
     9061 aggtagccca taaaggcttc cgccatcaat gccaagaaaa tcagagaacc gaaaatccac
     9121 accaactcac ggggtttttt gtacgaaccg taaatcaaac cacggaacat gtgcagataa
     9181 acaacaatga agaagaaaga tgcgccggtg gaatgcatgt agcggataat ccaaccgccg
     9241 gacacgtcgc gcatgatgta ctcgactgca gtaaaggcag caggcagatg gtaggcattc
     9301 aggttaccgt ccggtttgta gttcatcgtc aggaaaatac cgctgacgat ttgaatcacg
     9361 aggacaagca aggctaaaga gccgaagtag taccaaaaat taaagttttt cggtgcgtag
     9421 tattgagcca aatgctcatt ccacatttta gacaggggga aacgagcgtc catccagcct
     9481 aacaatgctt ttgctttgct attggtttgg tttgccataa ttatcgttcc ttattcttag
     9541 tcttcgccca ccaagatagt tgtgtcgctc aagtatttat atggcgggac aaccaggttg
     9601 gtcggggcag gaacaccttt atatacgcgg ccggccaagt cgaatttcga accgtggcag
     9661 gggcagaaga agccgccttt ccagtctgca cccaaatcgg cgggggcaat gtcgggacgg
     9721 aaggtgggcg agcagcccaa atgggtgcag ataccgatgg cgacaaggat gttcggctta
     9781 atcgaacggg tctcgttttt agcatactcc ggctgctgtt ccgcatcgga attgggatcg
     9841 gtaagttcgc cgttcaggcc tttcaggtct ttaagctgct gatctgtacg gttgagcacc
     9901 caaatcggtt tgccttgcca ctcggcggtc agtagctgac ccgcttcgat tttactgaca
     9961 tccacctcga cggcagcacc ggcggccttg gctttttccg aagggaaaaa actggccaca
    10021 aacggcgttg ccacacccaa tgctgccact ccgcccgcgc cgcaggtcgc gagtgtcagg
    10081 aaacggcggc ggccgttgtt gatttcttga ttatccatta ttcagtcgtc ctaatatttt
    10141 gggaataccg agccattaaa cgttgcaatt ttacccagtt tgcagtgata ctcaaagcat
    10201 tatttaaaat aaggtaaagt tttatgatat ttctcaagac tcaagccgga ttgttttcgt
    10261 caaaatggca cacttccaac ccgaaaacct ctgccgccga ttctgccagc gcgcgtacgc
    10321 cgtaacgttc cgtcgcgtga tgccctgccg aaatgaaagc cgtacccgtt tcattggcaa
    10381 ggtggtattg agcttcagag atttcccccg tcaaatacag atcgacacct tcgtctattg
    10441 ccgtctgaaa aaacccctgc gccccgccgc tgcaccatgc aacccgtcgg atttcgcgtt
    10501 cgggtttgcc gatgacgaca ggcttacgtt gcaaaactgt ttcaatatgc gccgccaatg
    10561 cgccgagtgt cttggcttgt ttcaggctgc ccgagttgag caggttttgt tcgccgaacc
    10621 gtttttctgt cgcaaaaccc aatctgtcgg cgagttgggc attgttgccc agtgtgggat
    10681 gtgcatccag gggcagatgg tagcctgcca tattgatgtc gtgccgtaac agtgcggcaa
    10741 tccgttcttt tttccaacca gtaacggtcg gcaactcgtt tttccaaaac ataccgtgat
    10801 gtaccaaaag caaatctgcc ttctgctcca cagcaaaatc aatcgctgcc ctgcttgccg
    10861 ttactgacgt aacgattttc ccgatatatt ccctcccttc aacctgcaaa ccgttagggg
    10921 cgtaatcttt aaacaacgct gtctgcaatg tttcattaca ccaagtcaga aaatccctgc
    10981 gcaataccat cttttttcct aatcgcttta aacaagcggg cattctaatc gcaaaatgtc
    11041 cggaattcac atttttccga tttgcacccg catatgaatt attttaatat gcgccggttc
    11101 aatatgccgt ctgaagcccc atggattcca ttatcgaatt gcgccacctc aaaaccctgc
    11161 tggcacttga agaaaccggc agcgtctccc ttgccgccaa acgggttttc cttacccaat
    11221 ccgccctttc ccaccagatc cgtatgctcg aaaaccacta cggcacgccg ctgttcgaac
    11281 gcaaatccac gcccttgcgc tttaccccgg tgggcgaaag gctgctgcgc ctcgcccacg
    11341 aactgatacc tcaagttgct gttgcagaat gggatttagc gcgaatcacg gaaggagagg
    11401 cgggagagct gcggattgcc gtcgaatgcc atacctgttt cgactggctg atgcccgcca
    11461 tgggcgaatt ccgcccgatg tggccccaag tcgaattgga tatcgtatcg ggattccaag
    11521 cggatcccgt cggactgctg ctgcaacacc gtgccgacct tgccattgtt tccgaagcgg
    11581 aaaaacaaaa cggtatttct ttccaaccgc tgtttgccta cgaaatggtc ggcatttgcg
    11641 caccagacca tccgcttgcc gccaaaaacg tttggacggc ggaagacttt atcggggaaa
    11701 ccctgattac ttatcccgtt cccgacgaga tgctggattt gcccaaaaaa atcctgattc
    11761 cgaaaaacat caacccgccg cgccgacaca gcgaactgac catcgccatt atccaactgg
    11821 ttgccagcag acgtggcatt gccgcccttc cttattggac agtcatgccc taccttgaaa
    11881 aaggctatgt cgtccaccgc caaattactg ccgacggact gcaaagcaaa ctgtatgccg
    11941 ccatccgtac cgaagatacg gacaagagct atctgaacaa tttttgccaa atcatacgcg
    12001 aacgcggttt tgcagatttg cccggactga gcgaactgga atcggtctga ccccttattc
    12061 aaccataccc ggcagttttt ctattttttc atgtatagtg gattaacaaa aaccagtaca
    12121 gcgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat
    12181 ttttgttaat ccactatact gtttttgatt tttgcccaat ctgtaatctt tagattgcca
    12241 atgggaaacc gtctactaca aataaaaaac cctgcgataa gcagggtttt ttgaatttcc
    12301 aacattaacg tttggagaat tgttttgcac ggcgtgcttt gcgcagaccc ggttttttac
    12361 gttcaacttc gcgggcatcg cgggtaacaa aaccagcttg agacaaggcg ggtttcaacg
    12421 cggcatcgaa gtcgatcagg gcacgggtaa tgccgtggcg gattgcgccg gactggccgg
    12481 tttcgccgcc gccaacaaca ttgactttga tgtcgaaaga ttcggcgttt tcagtcagaa
    12541 ccaagggttg gcgaacaacc attcggctgg tttcgcgtgc gaagaattcg tcaacgggac
    12601 gaccgtttac gatgatttga cctgtacctt taatcaggaa tacacgagcc actgaacttt
    12661 tgcggcggcc tgtgccgtag tagtatttac cgttcatgtc gcgtccttat ttcagttcca
    12721 aaactttggg ttgttgcgca gcatgggcgt gttccgcacc cgcatacact ttcagttttt
    12781 taatcatggc gtaacccaga ggacctttgg gtagcatacc ttttacagct tgttccaaag
    12841 cgcggcccgg gaattgctct tgcatttcgc ggaaagtgcg ttcgtagata ccgcctggga
    12901 aaccggaatg gcggaagtat tttttatctt cgaatttggc accggttaca cgcagtttgt
    12961 ccgcattgat aacaatgatg taatcgccgg tatcgacgtg gggggtgtat tcaggtttgt
    13021 gtttgccacg cagacggctg gcgacttcgg ccgcaacgcg acccaagact ttgtcttggg
    13081 catcgatgac gaaccattcg cgcttcacct cgtggggttt cgctgaaaag gttttcatag
    13141 tggaaatcca aatagatata gaaagttgta aattttaaag acaggattcg attttgtcaa
    13201 tcgcattacc gcgttacgga aggattttcc ggatttcggc agactgcata ctgctttttc
    13261 gggcggggcg gcggccaatg tgaaaaaccg catcgttgcg atgcggtttt gaatgggaat
    13321 ccccgcgaga gccgtttcgg ccgaatccgc ttgaaccttg ctgacaaggc ggctgcctcg
    13381 ggtagtttcg ggtgcgtccg caaaaggacg ctcgcgccca ctactgctcc cggcaacctt
    13441 aagcgaactt attggttcaa aggaatatat gccttcgcgg acaccgcagg gaaaaagggg
    13501 ttattcctgc gccaagcggg atagtgcttt ttggcaggcg ttgtccatat cggctatttt
    13561 acgcgcaaaa tcgccgattg ccaaatcgcc gccgttcagg gaggttttca acaggtcgtg
    13621 gacgacgttg agcgcggcca taatgacgat tttttcgctg tccgcgacgc gtccgccttc
    13681 gcggatggct tcggctttgc cgttgagcat tccgactgcc tgcaacagtg tgtctttttc
    13741 ttctgccggc gtgttgacgg tcagccgggc gtgcatgact tcgatgtgga cttgttcgat
    13801 gttcatcctt taatccttat tgctgcgttt cctgccattg ggggaggcgc gctgccagtg
    13861 cgctgatttt ttccctgctc tgttcgagca ggctgcggta tcgtgtattt tcttctgtca
    13921 ggctgtcgat tttgttttgc aggtcttctt tgagtttgcc gacttggacg agcagggctt
    13981 cgctgagttc gtcgacggcg gtttcgtgtt cgagtttttg ccgctcgtgc gcccgtttga
    14041 gttcggcgac ggtttctttg aggcggcggt tttcgctgac gagggtttcg aatttttgta
    14101 ccagcgtata aacgctgctt tcgagttttt cgatattttg tttcataacc ttacctgtcc
    14161 gtatgccgtc tgaaggcttc agacggcatc tgtctgttgt ttattcaaaa cgcgcgctgc
    14221 gttccatcag cctttcgacg acctgttgcg gggtcatttc tttgcggatg agttgcagca
    14281 gggtttgggt aatcggcatg tcgatttggt acttacaggc agtattgaag acttcttcta
    14341 tcgtgctgac cccttcggaa acgtgtccga tttcgacaag cacctgatgc agttccttgc
    14401 cttctgccaa acccaagccg acgcggcggt tgcgtgagag tgcgccggtg caggtcaaga
    14461 tgaggtcgcc gatgcctgcc agccccatca tggttttggg ctgtgcgccc attgcggagg
    14521 caaggcgggt gatttcagct aatccgcgcg taaccagtgc ggcacgggcg ttaagcccgt
    14581 actctaggcc atcggacaat ccggtggcaa tcgccataac attttttacc gcgccgccaa
    14641 ccgccacgcc gataacatcg gtactgccgt aaagcctcat gacggtcgtg ttgagctgcg
    14701 gtacgagttc ttcaatccac tcttggtttt cggaggcgag gacgacggcg cagggcagtt
    14761 gtttggcgag ttcctgtgca aaactcgggc cggagagtac gccgattttc ttattgtcgg
    14821 gcaatacttc tttcaatact tgaaaggtca gcagccctgt atcctgctcg aatcctttgc
    14881 aggcggcgag gacggggagg tgtcccgcgc cgtactgttt gagcagctct gcgctgcttc
    14941 tcaatccggc aacggaggtt acgataagga caagtccgct gtctttgagc gcgtctgcca
    15001 aatccgcaca cacttccagc gtttcaggaa aggggaaacc gggcagcccg tgcttgtttt
    15061 cacgttcttc cttcatttgg cggacttgat ctgcgttgcg cgtccatagg gatacgcggt
    15121 tgccgtgttg ggaaaaatgc agggcgagcg ccgtacccca cgaacctgcg ccgataacgg
    15181 taattttcat tggttgtctt tcaacatatc actgccgttc actttaaaac aatcgctgtt
    15241 tctctgcaag tgcagacagg gaaatgccgt ctgaaaggcg ttcagacggc attttgcccc
    15301 gatgcggcac tatcagcctg tattgcgcaa accttgcgcc acgccgttga tggtcaggtg
    15361 caccatcaga agggcgtgcg gattgtcggg ttctttacgc aggcgtttga gcatggcgac
    15421 ttgcaaaccg ttgagcgcgt tcaggtaggg aatcctcaaa gcgagcgaac gggcgaggct
    15481 gcggttgtcg cgcaaaagtt cttcggtttg cagcaggtcg agcagggctt tgcggctgcg
    15541 ccggtattct tccttaatca tcccgaagat gatttttgcc ttatcgggcg attcgctcaa
    15601 gccggcatag ttttccgcga gggtgatgtc ggttttcgcc atcacttgtt ccatattgga
    15661 gagcatggct tggaagaacg ggttgctttg ggcgtgttcg cgcagggcgg cgagcgtttc
    15721 gggtttgtct tcgcacaagg tttccaccgc gctgccgaaa ccgtaccaag caggcagcat
    15781 gaggcggttt tgcatccagg aaaataccca cggaatcgcg cgcaagtctt gaatccgcgc
    15841 taaggtcttg cggctggcag gacggctgcc gaggttgagg gtggcgattt cctgaatcgg
    15901 gctggtttgc aggaagtagt cgatgaagtc ggggtgggta atcagttcgc ggtagtattt
    15961 gaacgatacg tccgacaatg cctgcatcag tttggcatca gggtcttttt tatccggcag
    16021 gatgctggct tccaaagtcg cggcaaccaa ggtttccaag ttgcgttggg cattgccggg
    16081 gtcggcgtat ttggcggtaa tgacttcgcc ttgttcggtg atgcggattt gtcccgccac
    16141 gctgcccgcc ggttgggcga gaatggcttg gtaagaaggg ccgccgccgc gacctacgct
    16201 gccgccgcgt ccgtggaaca ggcgcatacg gacatcgtat tttttgaaga gttcgaccaa
    16261 gcccaattcc gcctgataca gacaccatga gctggtaacg tagccgccgt ccttgttgga
    16321 gtcggaatag ccgagcatga tttcttggat gtttccacgg ctttcgagca gtgcatcgta
    16381 ccagtcgagg cggaacatgg tttccatgac cggacaggcg ttttccaacg cttcaatggt
    16441 ttcaaacagc ggcacgatat tgatgcggct gtgcggtttg ccgttttcca ccgccaacag
    16501 gccggtttct ttcagcagca atgccaaggc gagcaggtcg ctgggttgtt cgcagttgga
    16561 aataatgctt tgtgttacgg catcttcgcc aaattcgtct ttgattttgc gcgcttcgtt
    16621 gaaaattgcc agttcgtggc gggtatggtc gctgtatgtg ataaacgggc tgtacagcgg
    16681 acgttgatgg ctcaattcgc gcaacagggc ggtttgtttt tgctcttcgt tcaggcggtt
    16741 gtagtcttcc aagcctgcgt gttggaaaag ctcggcaacc acatcggcgt gtttgcctgc
    16801 gtgttggcgc aagtcgagcg gcatcatgtg gaagccgaac acggacacgg aacggatgag
    16861 gtctgccaaa cggccttcgg caagcaggcg gctgccgttg tcgataaggg aacgttgcaa
    16921 ttttttcaaa tcatcgagaa attcttgtgc cgaagcatac ggctcgagaa agccgaattt
    16981 gcagcccatg cccaaaccga gcgcgcgcgc tttgcccata gcgcgcgcca taatgtaggc
    17041 gatggcgcgg cggtagggtt cttccgtgcg agcgatttct tcgtcaggcg agagggctgc
    17101 cagtgccatt acgtcgtcgt tgactttgac gcggcggatg gagagcggca gttcgcggta
    17161 aagtttgtcg agttcgccac ggtaaaaacg gaacactgcg tcggcgtggc ggcggaaggc
    17221 aaagcgcagg gtttcggcag agacgaacgg gttgccatcg cggtcgccgc cgatccagcc
    17281 gccgattttc aggatatcgg gaacgtccac atcgggatag gccgtctgaa agtcgtgttc
    17341 catcttgcgg tagagcttgg gcagggcttc gaaaaagctc atcgggaaga tggacacgcc
    17401 gttgttgatt tcgtcgttga cgctgagttt gtggcggcgc gtttcgctgg tctgccacaa
    17461 gcccagcagg atagtgtcga tttcgcggcg cagccgtgcc agcgcgtcgg cattggtgca
    17521 gcgttcgcgt tgcggcaaca gtgcgcggat gcggcggttg aagcttaaga cggtttggcg
    17581 ttgcacttcg gtcgggtgcg cggtcaaaac ggcggtaacg gacgtattgt ccaactgccg
    17641 ctgcaccgat ttgccgtcgg ctttccccgc tttgagcctg cggacggttt ccgtcaggct
    17701 gccttccgcg ccgccgcgtc cggcttcttc gtggatttgg cggcggcgtt cgtggtgcac
    17761 gtcttcggcg atgttcaaaa tctgggcgaa caggccgcag gccaaggtta aatcgtgggt
    17821 ttgttgttcg tccaattgcg gcaatacttt ttcaatcaat gccgcgctgt cgtcggaagt
    17881 ggacaagagt ttgactgttt cgacaaccaa cggcgaggct tcttcgtgca ggaggttgaa
    17941 cagggattgt ttcagaaatt ccgcgtccgc cgccaaagcc gcgtccttcg gattgttcag
    18001 aatatgcagt tgcatgattt ttctctcatt gccgtaaata ctgtaaatgt acctcaaatg
    18061 ccgcatccgt gccaaaccgt tcacacttta accgcccgtg tcccgaaatg ccgtctgaag
    18121 ttgaacgccg cccgacggca gcgttacaat cgcccgcaac tgttttttcc gaacatcatc
    18181 atgaccacga ccgaacacga caacgacgac gaactcctgc tgcggtacag ccgccacatc
    18241 ctcttggacg aaatcggcat cgaagggcag caaaagcttt ccgacgcaca tattttggtc
    18301 gtcggatgcg gcggtttggg tgccgccgca ctgccctacc ttgccgcttc gggtgtcggc
    18361 acgctgacca tagccgattc cgacacggtc gagctgcaca acctgcaacg ccaagtcgca
    18421 tttgacgagg gcgatgtcgg caagcccaaa gccgaagcct tggcaggccg tctgaaacgc
    18481 atcaaccata ccgtcaacgt ccgcgccgtc aacgaaaaac tcgacggctg tcgcctaacc
    18541 ggtttggttc aagccgccga catcgtttta gactgctgcg acaactacgc cacgcggcag
    18601 gcggtcaacc gtgcctgcgt gcagacgaaa acaccgctgg tttcaggggc ggcggtgcgc
    18661 tttgaagggc aacttgccgt gtaccgtccc gacttgcccg actcgccgtg ttacgcctgc
    18721 ctgtttgacg gcggatcggc ttcagacggc atctgttccc tcttcggcgt gttctcgccg
    18781 ctggtcggca tcatcggcag tacccaagcg gcggaagctc tgaaaatcct gctggatgcg
    18841 ggcgaaccgt cgcacggcag gctggcggtt taccgcgcct tggaaggggg ctggcaatat
    18901 ttcgacctgc cgcgcaaccc agaatgcccg gtttgcggcg cggaacggta aaaccctgcc
    18961 gccgtttcag acggcatcca aacggatgcg gaggaacggt tttaaaaatt taaaaattta
    19021 catttctttg caaaaaaaaa aaaaaaaaaa aaaaataaac ttaccttata attgcagttg
    19081 ttttagcaat gtctgtttcg cagactcatt gagtaagacg ttttccccgt aatgtgtttg
    19141 gccgtctgtc ccctttgggt tcggacggct tttttttggc tgtgtatgaa tacccggttg
    19201 gttttatctg tttacagggg aaaaccgctt atttccgttc gggcggaaaa cggttccatc
    19261 ggataaaagg cattttgtcc gactgattaa agttatatct taggtttaca tcggcggaat
    19321 attcaaacac agcctttttt aaggaaatcc ggatacggcg gcgcatcaat aatgcggcgg
    19381 aatctcgtcg cgcagggaat acggctcttg cgcgtcggga ttcctgtcct gcattttttg
    19441 atacagcagc ctcaactgag cctgctgcaa atccagcgtc tgccgcaatt ccgccaccat
    19501 cgcgttcagg ccggcgatta cgtcctcctg aagcgcggat tggatttcca attcgacaat
    19561 acggcgttcc aactcttgaa ccgcgtccat ttacagcacc atcgcggcaa tccagccggc
    19621 aatcagcagc gggatgttgt agtggatgaa ggtcgggata acggaatcgc ggatgtggtc
    19681 gtgctgcccg tcggcgttca gccccatcgt cggacccagc gtggaatcgg acgcgggcga
    19741 accggcatcg cccaacgccc ccgccgtgcc gacaatggcg acggtggcaa gcggcgaaaa
    19801 acccaaaccg acacacaaag gcacataaat cgcggcaata atcggcaaag tggaaaagga
    19861 cgaaccgatg cccatcgtta ccaaaagccc caccaccagc atcgccaatg ccgccatacc
    19921 tttgctgttg ccgaatatcg ccatactgct ttccaccagc ggctgaatat gcccggtcgc
    19981 attcatcacg gcggcaaaac cctgcgcggc aatcataatg aagccgacca tcgccatcat
    20041 cttgatacct tcgccgaata cgtcgtttgc cttgtcgcgg ttgatgaccc ccaacatcat
    20101 aaatacggcg aaaccgagca tcgcgcccaa caccagcgag tcttcataca tcaactggat
    20161 ggcaaagcat acggcaatgg cggcggcggc ggccaggctg cggtaggcgg acggctgcgg
    20221 acggtttgcc gcatcggcgt tgcccgccgt atcggcattg ttgctttggt acaggcgcgg
    20281 tttgcggtaa tggacaaacg ccagcaggag tccggccagc attcccaacg cgggaatcgc
    20341 cattgccgcc atcacgttaa tgtttttcac atcaagctgc ggcgcggcgg aatggatgtt
    20401 gcccaacagg atttcgttca aaaaaatcgc gccgaagccg taaggcagga acatataagt
    20461 cgtaaccagc ccgaaagtga tgacgcacgc aatcaggcgg cggtcgattt tcaggcggtt
    20521 gaacaccaaa agcagcggcg gaacaatcat cgggataaag gcaatgtgga tggggatgat
    20581 gttctgactc atcatgccca tcacaaggat gatggaaagc agcagccatt tgaccgcgcc
    20641 ctcgcccgaa cgcacgctgt cgggcatacc gccccggttc agtttgcgga cgaccgcgcc
    20701 ggcaagctgc tgcggcaggc cggaatgggt aatcgccatc gcaaacgcac cgagcatcgc
    20761 ataagaaagc gcaatcttcg caccaccttc caaacctttg ttaaacacgg ggataatccc
    20821 cgcctgactg acctgtcccg ccgcatcggc aatgttttgc agcggcatac ccgccaccgc
    20881 gccgccgaca aacgcgccga tcgtcaggct caataccacg tgcacgcgcg acagcgacag
    20941 caccagcata acgattacgg caactacgac tgcattcatt gtttatcgct ccaaacctat
    21001 aaatgtttac atatcgaaac acatcataac ccaataacgg gaaacccgcc aattttgcaa
    21061 acaattattt caaatgcttc atatacttcc ccagcgtaac cctgtccaaa cccgccaaat
    21121 ccggcagggt ttccactcct gaaaaaccat tctccgccaa cacgccgcgc accgccgcgc
    21181 cctgatcgaa accgtgttcc agcaataaaa aaccgccttc cgccaaacgg tcgggcgcgc
    21241 cttgcgccaa ggtacggatg cagcttaggc cgtctgaaaa gtcggtcagc gcgatttgcg
    21301 gctcaaaccg caaatcgcct tgcaacaaat gtttatcgcc gttttcgata tagggcgggt
    21361 tggacacgat gatgtcccat ttcccttcag acggcatatc ggtgtcgaac cacgaaccgt
    21421 gtgcaaattc gacccgcgcg cccaaatccg ccgcattttc ccgcgccgtt tcaagggcgg
    21481 gcgggctgat gtcggatgcg cgcacaaacg catcggggcg ttcgagcgcg acggttacgg
    21541 caaccgcgcc gctgcccgtc cccaaatccc acacgcgccc gttttcgggc aggcgcgcca
    21601 atacggcttc gaccaaatgt tcggtttcgg ggcgcggaat cagcacgctc ggattgactg
    21661 taaagcgtct gccataaaat tcgcgcacac ctaaaatata ggcaaccggc tcgccgttca
    21721 gacggcattg cgccagcctg tccgcccgct gtcggacttc gtccggcatt tcttccccgc
    21781 cccgcgtcaa caactgcacg cgcgtatatt ccgaaacata ttgcagcagc attcttgctt
    21841 caattttagg cagttttgac aagcccaacc atttatcaaa cgtcattttt tatcccgtct
    21901 gccgccggtg cggcttttct ttccttattc tttccggcaa acgtaccgat ggtggcaacc
    21961 gcaaatgcgg cataccacaa ataaaatcct gcaccgtagc gcacaatatc cgatgtattc
    22021 cctgcttcat cgacgtatac ggctttcaca ctgaaagcca ccaacgccaa gccccaaaat
    22081 gccgcatgga caggcacgac cttcttccgc aacgccagca aaacaatggc cgccaaccaa
    22141 acataattcg catagaccgc acaatacctg atatccaaag aagcaaatat cgaccccaaa
    22201 atcaaaacgg tcaaaccctc catgcttcca tgattgccca aataaaatgc aacattggat
    22261 aaagacgcta tccacagggc aaccgacacc agcaacatca ctatgggaaa acttgatttc
    22321 cgattctgtt cctgcatggt tttatcctaa tgtaaaaggc cgcctgaaaa cctttcagac
    22381 ggcatcgtgc cggattccgc gtcagattgc gctgccgccg acggtcagcc cggcatcgat
    22441 acgcagggtc ggctgcccca cgccgacggg gacgctctgc ccttctttgc cgcacacgcc
    22501 gacaccgctg tccaacgcgg tatcgttgcc tatcatcgaa acgtgtttca gcacttcggg
    22561 cccgttgccg ataatggtcg cgcctttgac ggggtattgc agcctgccgc cttccaccca
    22621 ccatgcttcg gacgcgctga acacaaattt gccgctggta atgtccactt gtccgccgcc
    22681 gaaattgacg gcgtaaatgc ccttgtcgat ggacgcgatg atttcttccg gctcatagct
    22741 gccgttttcc ataaaggtat tggtcatgcg cggcataggg gcggaagcgt aactttcgcg
    22801 gcggccgttg ccggtggatt gcgtacccgt caggcgggca ttggtttcgt cctgcatata
    22861 gccgactaaa atgccgtctt caatcaatac ggtgcggcgg gtttcgttgc cttcgtcgtc
    22921 gatgttgagc gaaccgcgcc ggccggcaat atcgccctga tcgacgacgg taacgccttt
    22981 ggcggcgacg cgctcgccta ttctgccgga aaagacgctg gtccccttgc ggttgaaatc
    23041 gccttccaaa ccgtgtccga ccgcttcgtg cagcaacacg cccggccagc cgttgcccaa
    23101 aacgacggtc atttcgccgg cgggcgcggg gcgggattcg aggttggtga gtgcctgttt
    23161 gacggcggca tcgacaaact gccgcaccaa gttttcatcg aaataagcca agtcgtagcg
    23221 tccgccgccg cccgcgctgc cctgttcgcg gcgttcgccc tgtttggcga taacggtaac
    23281 gttcaggcgc accatcgggc ggatgtcggc ggcgtgtttg ccgtccagac gggcgaggta
    23341 aaccatatcg tattcgcagg tcaaaccggc catcacttgc acgatgcgcg gatcggcggc
    23401 tttggcgatt gcttccactt tgttcaacag cgcgactttg gcggcagaat cgaggccggc
    23461 aatggggtcg gacgcggaac aaaccggctt gccgcgcgtt tcagacggca ttttggcgga
    23521 caccttgccg cctgccgccc caatcgcgcg gacggcgcgg gcggaacggt ttatcgaatc
    23581 gatgcacagg ctgtcggcgt aggcaaaggc ggttttgtcg cccgaaacgg cgcgcacgcc
    23641 cacgccctga ttgatttgga agctgcccga tttgacgatg ccctcttcca aatgccagct
    23701 ttcataagcg gtgcgctggc agtagatgtc ggcgtaatcg acgtggtgcg cgccgatgat
    23761 gcacaggctt ttggcgagca gttcggggga aaggcggttg gcttcgagca gccgcgcctg
    23821 tacggcggaa taggtcggat gcatagtgtc ggcgcataaa aaatcagggg cttgattata
    23881 cggcatttgt tatatagtgg attaacaaaa aacagtacgg tgttgcctcg ccttgccgta
    23941 ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatagaa
    24001 atgcgccgtg ccgccgaaaa tgtaagaatt ttgccaacgc acgttgcttt gtgtaaactt
    24061 aaagctttct tgtcggagtg ccgccgccgg gcggctgaga ttgcgaaagc agaatccgta
    24121 gaacctgtcg gggtaatgcc tgcgtaggaa acaaaccgtc aaatgcctta tcaggcttcc
    24181 gttccctttt ccgcacttcc ccgcccacct tccttctttt taaaggacgt catatgtcgg
    24241 gcaatgcctc ctctccttca tcttccgccg ccatcgggct gatttggttc ggcgcggcgg
    24301 tctcgattgc cgaaatcagc acgggtacgc tgcttgcgcc tttgggctgg caacgcggtc
    24361 tggcggctct acttttgggt catgccgtcg gcggcgcgct gttttttgcg gcggcgtata
    24421 tcggcgcact gaccggacgc agctcgatgg aaagcgtgcg cctgtcgttc ggcaaacgcg
    24481 gttcagtgct gttttccgtg gcgaatatgc tgcaactggc cggctggacg gcggtgatga
    24541 tttacgccag cgcaacggtc agctccgctt tgggcaaagt gttgtgggac ggcgaatctt
    24601 ttgtctggtg ggcattggca aacggcgcgc tgattgtgct gtggctggtt ttcggcgcac
    24661 gcaaaacagg cgggctgaaa accgtttcga tgctgctgat gctgttggcg gttctgtggc
    24721 tgagtgccga agtcttttcc acggcaggca gcaccgccgc acaggtttca gacggcatga
    24781 gcttcggaac ggcagtcgaa ttgtccgccg tcatgccgct ttcctggctg ccgctggccg
    24841 ccgactacac acgccaagca cgccgcccgt ttgcggcaac cctgacggca acgctcgcct
    24901 atacgctgac gggctgctgg atgtatgcct tgggtttggc agcggcgttg ttcaccggag
    24961 aaaccgacgt ggcaaaaatc ctgctgggcg caggtttggg tgcggcaggc attttggcgg
    25021 tcgtcctctc caccgttacc accacttttc tcgatgccta ctccgccggc gtaagtgcga
    25081 acaacatttc cgcgcgtttt gcggaaacac ccgtcgctgt cggcgttacc ctgatcggca
    25141 cggtgcttgc cgtcatgctg cccgttaccg aatatgaaaa cttcctactg cttatcggct
    25201 cggtatttgc gccgatggcg gcggttttga ttgccgactt tttcgtcttg aaacggcgtg
    25261 aggagattga aggctttgac tttgccggac tggttctgtg gcttgcgggc ttcatcctct
    25321 accgcttcct gctctcgtcc ggctgggaaa gcagcatcgg tctgaccgcc cccgtaatgt
    25381 ctgccgttgc cattgccacc gtatcggtac gccttttctt taaaaaaacc caatctttac
    25441 aaaggaaccc gtcatgaccc gtatcgccat cctcggcggc ggcctctcgg gaaggctgac
    25501 cgcactgcag cttgcagaac aaggttatca gattgcactt ttcgataaag gctgccgccg
    25561 gggcgaacac gccgccgcct atgttgccgc cgccatgctc gcgcctgcgg cggaagcggt
    25621 cgaagccacg cccgaagtgg tcaggctggg caggcagagc atcccgcttt ggcgcggcat
    25681 ccgatgccgt ctgaacacgc acacgatgat gcaggaaaac ggcagcctga ttgtgtggca
    25741 cgggcaggac aagccattat ccagcgagtt cgtccgccat ctcaaacgcg gcggcgtagc
    25801 ggatgacgaa atcgtccgtt ggcacgccga cgacatcgcc gaacgcgaac cgcaactcgg
    25861 cggacgtttt tcagacggca tctacctgcc gaccgaaggc cagctcgacg ggcggcaaat
    25921 attgtctgca cttgccgacg ctttggacga actgaacgtc ccttgccatt gggaacacga
    25981 atgcgtcccc gaagacttgc aagcccaata cgactggctg atcgactgcc gcggctacgg
    26041 cgcaaaaacc gcgtggaacc aatcccccga gcacaccagc accctgcgcg gcatacgcgg
    26101 cgaagtggcg cgggtttaca cacccgaaat cacgctcaac cgccccgtgc gtctgctcca
    26161 tccgcgttat ccgctctaca tcgccccgaa agaaaaccac atcttcgtca tcggcgcgac
    26221 ccaaatcgaa agcgaaagcc aagcccccgc cagcgtgcgt tcagggttgg aactcttgtc
    26281 cgcactctat gccgtccacc ccgccttcgg cgaagccgac atcctcgaaa tcgccaccgg
    26341 cctgcgcccc acgctcaacc accacaaccc cgaaatccgt tacaaccgcg cccgacgcct
    26401 gattgaaatc aacggccttt tccgccacgg tttcatgatc tcccccgccg taaccgccgc
    26461 cgccgtcaga ttggcagtgg cactgtttga cggaaaagac gcgcccgaac gcgatgaaga
    26521 aagcggtttg gcgtatatcc gaagacaaga ttaaagccgt ctgaaaggaa ccccatgacc
    26581 ttcctgccac tcaaatcccc gctcaaattc tacgccgtcg tccccaccgc cgattgggtc
    26641 gagcgcatgg tcgaagcagg tgccgacacg gtgcaactgc gctgcaaggc cctgcacggc
    26701 gatgaattga aacgcgaaat cgcccgctgc gccgcagcct gccgtggcag ccgtacgcag
    26761 cttttcatca acgaccactg gcgcgaagcg atagaagcag gcgcgtacgg cgtgcatctc
    26821 ggacaagaag acatggacac cgccgacctc gccgccattg ccgctgccgg tttgcgcttg
    26881 ggtttgagta cgcactccgt cgccgaactc gaccgcgccc tgtccgtgca ccccagctac
    26941 gtcgccagcg gcgcgatttt cccgaccacg accaaacaaa tgcccaccgc ccctcaaggc
    27001 ttggaaaaac tgcgtgaata tgtgaaacag gcaggcagca cgcccgtcgt cgccattggc
    27061 ggcatcgatt tgaacaacgc ccgagccgta ctcgccaccg gcgtttcctc actcgccgcc
    27121 gtccgcgccg taaccgaagc ggcaaatccc gaagcggtgg ttaaagcgtt tcaggctttg
    27181 tgggatggat aaaaccgaaa gaagaaaatt caattgccgt gtcggcaaaa cttagcccgt
    27241 tatcgcaaac atacttaact ttaaatgtgg catatcatca aattccgtca ttcccgcgta
    27301 agcgggaatc cgccttaaaa cttgagaaac catcatttga aaaacagttt ccgaatttca
    27361 aaaatggatt cccgcctgcg caggaatgac ggcaaccggc cggttgcgta tcaaaaaata
    27421 aagtaattcg gctagatata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
    27481 acagtacaaa tagtacggaa ccgattcact tagtgcttca gcaccttaga gaatcgtttt
    27541 ctttgagcta aggcgaggca acgccgtact ggtttttgtt aatccactat aaatacagaa
    27601 acatcgagaa accatgaaca tcatcttaaa cggcgaaccc gccgaacttc acggcacaag
    27661 cgttgccgac ctcatcgccc aaaccgcgcc gcaaaagccc tttgccgtgg cggtcaacac
    27721 cgttttcgtc cccaaaggcg cgtatgcgga aacggtttta aacgaaaacg acaaaatcga
    27781 tatcgtgcgg ccggtggtcg gcggctaggc ggttttgcct tttcagacga cccctgtccc
    27841 caaaacaacg ttatggtgga ttaactttaa atcaggacaa ggcgacgaac cgcagacagt
    27901 acaaatagta cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttttctttg
    27961 agctaaggcg aggcaacgcc gtactggttt ttgttaatcc actatacaaa ggaacccatt
    28021 atgctcaccc tatacggaga aactttccct tcacggctgc tgctcggcac ggctgcctac
    28081 ccgacccccg aaatcctcaa acaatccatc caaaccgccc agcctgcgat gattaccgtc
    28141 tcgctgcgcc gcgcgggaag cggcggcgag gcgcacggtc aggggttttg gtcgctgctt
    28201 caagaaaccg gcgttcccgt cctgccgaac acggcaggct gccaaagcgt gcaggaagcg
    28261 gtaacgacgg cgcaaatggc gcgcgaagtg tttgaaaccg attggataaa attggaactc
    28321 atcggagatg acgacacctt gcagccggat gtgttccagc ttgtcgaagc ggcggaaatc
    28381 ctgattaaag acggcttcaa agtgctgcct tattgcaccg aagacctgat tgcctgccgc
    28441 cgcctgctcg acgcgggctg tcaggcgttg atgccgtggg cggccccgat cggcacgggt
    28501 ttgggcgcgg ttcacgccta cgcgttgaac gtcctgcgcg aacgcctgcc cgacacgccg
    28561 ctgattatcg acgcgggctt gggtttgccc tcacaggcgg cacaagtgat ggaatggggc
    28621 tttgacggcg tgcttttgaa tactgccgtt tcccgcagcg gcgatccggt caatatggca
    28681 cgcgccttcg cactcgccgt cgaatccgga cggctggcat ttgaagccgg accggtcgaa
    28741 gcacgcgaca aagcgcaagc cagcacgccg acagtcggac aaccgttttg gcattcggcg
    28801 gaatattgaa aaaggcggca aaaatgccgt ctgaacggtt cagacggcat cgcagcccaa
    28861 aacggcggcg gtctgaaacg ggcaaaccgc cattcccaag catcacggct ctgtcggaaa
    28921 aaatggaaaa accggccgga aaaccttgcc gcccgtcccg atgccgcaac caacgaaaca
    28981 ctcggcctcc acggtgtgca ggctgccgcg caagccctaa atacggcaat agaagaagcg
    29041 ttttgttgtt gtttgaatac acttaaaaat acctaaagcc gtccgtaagc cttcatccgc
    29101 aacggtttta ccgcttttgc atccccgaat ccacgctcaa acacccccga atgacaaccc
    29161 tgtccgcgcc aaatcggacg gatgttcaaa cacggcaggc ttatttccgt caggcacgaa
    29221 gccctcagct atgactgccg accccgattt gtccgacaca atgaaagttt gccgacccga
    29281 atcacaaaca tcggcggaca ggttaatttg tttatttttc atcgtattac aaaaaatccg
    29341 catttatttt taaattttta ttgataatta ttattattag cgtataatca aaaccactcg
    29401 gaagtcgtcc attccgaacc attaaacact gtattccctc acaattcact ttcacacttg
    29461 gagtcggcat atacgagaca tacattccct ttttatatat cagatactca aaaccgaaac
    29521 gccaaaccca ccttcgcggt gggtttggcg tttatcgtcc ggctttcgcg cctatttgca
    29581 agacttgagg ttcagtttgc cgtataggga cgtgatttta cgaatttcgt ccgcatcggc
    29641 ggcattcacg ccggtaaaca aaaccgtcat acgcgacacg ctcaaagaat cgtcctgcct
    29701 gtcggcttcg gcaaagtgtt cgacaatatg cgccccgccg aatcctgcgc cggcaagccg
    29761 gttttgcagg gcttgggcgt tttcccggct gttgccgaca cccaaactca atgcgccgtc
    29821 aaacggtatg gggttgaaac ctttggcaga cagctccgcc gcctgatttt cggcatcggc
    29881 ggaaacgggc aggacgacgc gataggtttt gtcggcaggt ttggctttgg cggtgcgttt
    29941 ttcgacgctc ctgctggcaa cgtgcgacca tttgcccaaa agtcctttga tgcggtggta
    30001 gtcatcttcg tccatcgtga ggcttgcctg cgcggcgcat aaggcatccg ccgcgccgtt
    30061 ttcgcgttcc gcctgcgcct tttctgcggc gagtttttcg cggcgggctt tttcttcacg
    30121 ctgttttttc tccctcagtt ttttctgttc cgcttccttt ttcaagcgca actgctcggc
    30181 ctgctcttcg gtcaggatgt cgcccggttt gagcagtgcc gccgtatcct ccacggatgc
    30241 cgccgcattg tcggcggcgg gcgttgcggg gggcgtgttt tcgacggccc ggttttccgg
    30301 cacgccgccc gtcgctttaa cggctaattt atagcccacc gtgccgccga atacggcaat
    30361 attgagtacc gccaaaaggg tgaagagcca tttcattaga atttcctcaa tgtttgaaac
    30421 cccgcccgcc tgggcggcag tctgtcgggc ggcagaatcc ggacggattc ggacggatgc
    30481 cgccccgtgt tgaaaaaggg tgtatttttt aagtatgttc cgattccccg ccttcggcgg
    30541 caatcaggtt cagcagcccg tgaatgacga ggttgtccgc cacgcgcacg gtattttccg
    30601 ccaaaaatgc aggcggcagg gcttcggcaa cttttgccgc gccgccgccg gtaatgatga
    30661 catcgacagg cttgcccgcc ccggtttttt ctttcaaacg cccgtgcatc atcataaccg
    30721 agccgcaaac cgcatccatc atgccgctgg cgacggcatt gcccgttgtg gtcgggaaag
    30781 gataacgctt accggcgtgc cggttgaggt tggcggttcg gacggcgagc gattctttca
    30841 tcaggtggaa accgggcatg atggttcccc cgagataatg tccgtcatcg gtgagcgcgt
    30901 caaccgttac cgccgtgccg caactgacga cgacgcaggc attgcggctg aagcggcggc
    30961 tgcccaaggc gttgaaccag cggtcggaac cgtgttcttc ggggtggcgg tagtggttgc
    31021 gtatgcccaa agcctgtgcg gaagacggca gccactcgat ttttcgggcg agctgttcct
    31081 gcacttgtgc ctttttgaat tctccgcaca cggcgcaacc gacgatgcgg acatttccat
    31141 ccgccttttc cgcccactcc gcgcccaaag gcgacaaatc gcggtacggc gcgctaccga
    31201 cggttgcgaa cgtgccgttt tccacccacg cccacttgag ccggctgttg ccgccgtcca
    31261 acagcagaaa acgttccgaa tcccgccgct tcggcacgga aaccggcctg tcatcggacc
    31321 gcaggctgat ttcgccgctg acgaccgtct gtttgccctc tgccgtttcc aagtgcaaaa
    31381 cgccttgtcc gtccacgcct ttaaccgtgc cttcgaacac ggtttcgccg tcgcgcaaca
    31441 gcaataccgc cttgccgtgg tcgcggttgg cagcctgata ttccgccaca aaaggcgcaa
    31501 atccgtcccg cgcatattgc aacaacaccg catcaagttc cgccaacagc gtttccagca
    31561 acacggcggc atcggcattt ccccgccgcg atgccgtctg aaacagcgat tgcacggaag
    31621 cggcattttc cacttccttg ggcaggacaa aattgatgcc gataccgacg acggcaaccg
    31681 tcttgccgcc cgccctgacc gtttcaatca gaatgccgcc caatttatcg cgccccacga
    31741 ccaaatcgtt gggccacttg atttgcgttt tcaaacccaa acgcgacaag gcgcgccggc
    31801 acgccactgc cgcaacaggc gacagcgaac ccaactcata ctgcggccgg tcaaacaccc
    31861 agccaaaact gaacatcaga cactcgccca aacggtgcga ccacttccgc ccctgccgcc
    31921 ccctgccctt actttgcagg tgggtcacac atatggtttt gtgcgccttg tccggcgcaa
    31981 tccgcgccaa ttccagtatc tcgtcgttgc tggacgcgca ctcgtgcttc aatgccgtct
    32041 gaaaacccga cctttccccc agctcgcgca aaccttcggc atcgaaaacc gccaatgggc
    32101 gcaccagccg ccaatagccg tcgtgttggc gcaacagccc gcgtatgtgc gccggcatct
    32161 gctgccaaaa accgttgagc tgctgcggct tcatatccgc catacgcgcc agttgcgaga
    32221 cgtgttgcgg caaaccgtcg gcaagctccg ccaacacccg ccagtgcgaa agcttcaaaa
    32281 ccgtcatttt ccgccctctg ccgcacggat ttttgccaaa gtcttcgttg tcgaagtctg
    32341 gtgcagaaac ggaattgaaa acacctgacc gccgcgcgcc aacgtttctg ccgcaccgac
    32401 aatcttatcc gcagcccaat cgccgccctt gaccaaaatc tcaggtttga ccgcctcaat
    32461 caacgccgcc ggcgtatccc cgtcaaacca cgttaccaaa tccacacttt ccaaagcggc
    32521 ggcaacggcg gcacggttct ccaaaggatt aatcgggcgg tcgccgccct tgcccaaacg
    32581 ccgcaccgaa gcatcggtat tcaacgccag caccaacgcg ccccccatcg aacgcgcctg
    32641 cgccagataa gtaacgtgcc ccctgtggag gatgtcgaaa cagccgttgg taaacaccag
    32701 cgggcgcggc aacaacgcca aacgcgccgc caacgcctcg ggcggacaga ttttcgattc
    32761 aaaatcaggg acagaccaag cgtcaaccat caaagcctcc gacaaaaacc ataaaagaca
    32821 gaaaaaccca catgatacag aagcatatgc gaaaggcaaa gccggcggcg cggacagtac
    32881 gcgcaaacga gaaaagaccc gtaccgaaaa gtacgggcct ttatctgggg tggctgatgg
    32941 ggctcgaacc cacgacaacc ggaatcacaa tccggggctc taccaactga gctacagcca
    33001 ccataaaaac ggttttcaat caaattcttg gcacgcccga caggaatcga acctgtaacc
    33061 cccgacttag aaggtcggtg ctctatccgg ttgagctacg ggcgctcatg ccgattcgtg
    33121 ctgattgatt ggtcggggcg gtgggattcg aactcacgac cctctgctcc caaagcagat
    33181 gcgctaaccg ggctgcgcta cgccccgact tgaagaagcg aactatacaa ctcagggaaa
    33241 gatgcgtcaa tatttatttt caagacacca agatgaaaaa tatagttttt tgatttgaaa
    33301 aaatatttaa tccgtccaaa cagccgtatt ttatttcagg gcaaatttat tttcggcatc
    33361 ctgctgtaaa aacaaacgga aaatgcgata attttcagca ttttctacct gtttaacaaa
    33421 aggacggata tgtcggcaca actgatcaat ggtaaagaag tttcgcaaaa acgcctgcag
    33481 gcggttgccg aagcggtggc gcaacgccaa cagaacaatc tgcacacgcc ttgcctggcc
    33541 gtggttttgg tcggaggcga ccctgccagc gcggtttatg tccgcaacaa gaaaacctcc
    33601 tgccaaaaat gcggcatcaa atcactgtct tacgagctgc ccgaatcaac atcgcaggaa
    33661 gaactgctgg cactggtcga ccgcctgaat gtcgattccg aagtggacgg tattctggtt
    33721 cagctgccac tgccgaagca cctcgacagc caggcggttt tggaacgtat ttcgccggat
    33781 aaggacgtgg acggcttcca tccttacaat gtcggcaggc tggcggtcaa aatgccgctg
    33841 atgcgcccgt gtacgcccaa gggcgtgatg acgcttttgg aggcttacgg catcgatccg
    33901 aaggggaaaa aagcggtcgt ggtcggcgcg tcgaatatcg tcggccgccc gcaggcgttg
    33961 gaattgctgc tggcgcgcgc aacggtaacg gtctgccaca gcgcaaccga aaatctggca
    34021 gacgaggttg ccggagccga tattttggtg gtcggcgtgg gcattccgaa ctttgtcaaa
    34081 ggcgaatgga tcaaacccgg cgcggtcgtt atcgatgtgg gcatcaaccg tttggacgac
    34141 ggcagcctgt gcggcgacgt ggaatttgaa acggcaaaag aacgggcggc gatgattacg
    34201 cccgttcccg gcggcgtggg tccgatgacg attgccacat tgatggaaaa caccctgcac
    34261 gcggcttcac tgcacgatgc ttgagcggtt ctgaagataa aaatgccgtc tgaaaggctt
    34321 tcagacggca ttttgccgtg tccgtttatt tgggcagctt gacgacaacc gtatccgcca
    34381 gtatgtcgta aagcgtgcgg cggtcgcgtt tgaccataaa gagcaggaca aagttggcaa
    34441 ggaatgccag caggttgatg gcgttttctc cgttgtcacc tactgcaaga ccgataacgg
    34501 cggcaataat ggcaaccaaa accgaccatg cgatttcgcg taccaaaacc gtgccgacaa
    34561 aacccggatt gcggccgtcg gttttcaaca ccctgattct catgatcttc ttacccaatg
    34621 actgcccgtc ccgactcata tagtagattt gaacgacggt ataaaccaaa atgcccgcca
    34681 gtcctaccca aaaggaagtc atgcccaaaa gcagcccgaa tatttcctca ctgccgccaa
    34741 tcctgccttc attcttgatg gcgaaagcaa tcagtccggc aaacggcacc aacaaaacca
    34801 aaaaggtaaa caattggttc agcagcgcgg caagtatccg gtcgcctgcg ccggcaattc
    34861 cgacttcaat ttcctgcccg ttgcggttgt cggatgccgc gtcggtgtag tcgttttttt
    34921 cttccatatc cgttcctgat aattgttctt aactgacccc gattctaccg ccacgacacc
    34981 gaaaacgcca atacttaaag aaatcccgat aaagaacttt acattttccc aatacggcgt
    35041 taaaacgctt cctttacgcc atacataatt ttatagtgga ttaacaaaaa tcaggacaag
    35101 gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgcc gtactggttt
    35161 ttgttaatcc gctatattaa cgattttttc ctcaaggagc aacacaatga aagtaggttt
    35221 cgtcggctgg cgcggtatgg tcggttcggt tttgatgcag cgtatgaaag aagaaaacga
    35281 cttcgcccac attcccgaag cgtttttctt taccacttcc aacgtcggcg gcgctgcccc
    35341 tgatttcggt caggcagcca aaacattgtt ggatgccaac gatgttgccg aattggcaaa
    35401 aatggacatc atcgttacct gccagggtgg cgattacacc aaatccgtct tccaacccct
    35461 gcgcgacagc ggctggaacg gctactgggt tgacgcggct tcttctctgc gcatgaaaga
    35521 cgatgcgatt atcgtcctcg accccgtcaa ccgcaacgtc atcgacaacg gcctcaaaaa
    35581 cggcgtgaaa aactacatcg gcggcaactg caccgtttcc ctgatgctga tggcgctggg
    35641 cggtctgttc caaaacgatt tggtcgaatg ggcgaccagc atgacctacc aagccgcttc
    35701 cggcgcaggc gcgaaaaaca tgcgcgaact catcagcggc atgggcacga ttcacgccca
    35761 agtagctgac gagcttgccg atccttccag cgcgattctc gacatcgacc gcaaagtgtc
    35821 cgatttccta cgcagcgaag actatccgaa agccaacttc ggcgtaccgc tcgccggcag
    35881 cctgattccg tggattgacg tggatttggg caacggccag tccaaagaag aatggaaagg
    35941 cggcgtggaa accaacaaaa tcctcggccg cagcgacaat ccaaccgtga ttgacggcct
    36001 gtgcgtccgc gtcggcgcga tgcgctgcca cagccaagcc atcactctga agttgaaaaa
    36061 agacctgcct gtttccgaaa tcgaaacgat tttggcaggc gcgaacgact gggtgaaagt
    36121 catccccaac gaaaaagaag ccagcatcca cgagctgact cctgccaaag ttaccggcac
    36181 gctgtccgtc cctgtcggac gcatccgcaa actgggcatg ggcggcgaat acatcagcgc
    36241 gttcaccgtc ggcgaccagc tcttgtgggg cgcggccgaa ccgctgcgcc gcgtattgcg
    36301 tatcgtgttg ggcagcctgt gagccctgtt tgaatggaaa tgccgtctga agcctgtttc
    36361 agacggcatt ttccttgcaa ccctgccgga taacgccctg ccggcactgc cgacgtaaaa
    36421 aataaaggat tccatttccg gcggtatgcg gcagcccgac tttatccgaa cctgatgcgc
    36481 ctgcacgtca atgaaaacag cccgattgcg gacttcctgc tacagccgaa attccgataa
    36541 ggcaagcgtt cacgccagca acatttcctg catcagcttc ataccccact gccagccgcc
    36601 gagcatgccg ttcaaactgc ccgaatgcgg ggaaaccaac aggcgggcgt tccacaaatc
    36661 cgcctgtttt tgcgcccaac cgtgcggcac gccgccgtgt tcgggtacaa ccaatgcggc
    36721 acggcaggga cagcggacgc gttggaaagt gtgttccgca tcgtcgggaa aaatatcggg
    36781 acgctgcggt acaaggatga tgttggcaat tttcttccgt gtcaggatgt ctgcctgata
    36841 cagccacgcc aaaaatgcgg ccgcgcccgc accgtgtgcg acaacggcga cgtatttgcc
    36901 acgtatgcgt tcaaatgccg tccgaagccc tgcctgccat tcccctatgc tttgaccggc
    36961 cgacgcttcg gacatctgca cgacgggata actgatcgcc caacggtcta tccacatttc
    37021 cgcttcatcc gcatcgcgta ccagccacag cgtcaaatct tcgagttcaa aaccctgcat
    37081 accgccccgc ctatttcagc aggtcccgga gggtaaaggc gatgagcagc gaagcgggta
    37141 cgctcaatat ggcgcagacg gtcaggcagg caaaaatatt caccacccgc cgccagcctt
    37201 tccaacccaa acatttggac gcaatcagca ggcagggcat gcagatcagc agccacacca
    37261 acgcccatat cgggtttacc ttggtcggcg caagccagcc ttgcatccgc gacaacataa
    37321 atatcgccca caccaatatg ggcaggataa acgcagcgac aacccatgct gcgcctattc
    37381 ctgtttttcc gtccacattc caatcatatt tacccaaaac tttattcggc agcatagtca
    37441 tactccatga ccagcggcgc atggtcggaa aatttttcat ctttataaac gtgtgcggac
    37501 acggctttgg cggcaagttc gggcgtaacc atctgataat cgatgcgcca cccgacatct
    37561 ttcgcatacg cctgcccgcg gttgctccac cacgtgtagc ccggcacatc gggataaagc
    37621 gtgcgccaca tatccgtcca accgagcttg tggataacct tgcctatcca ctcgcgttct
    37681 tccggtagga agcccgaatt tttctgattg cctttccagt ttttcaggtc gatgttttgg
    37741 tgggcgatgt tccagtcgcc gcaaacgaca atgtcgcgtc cttcgttttt catcgcttcg
    37801 agcatagggt aaaacgcatc aaggaaacgg tatttcacct gctggcgttc ttccgcgctg
    37861 ctgccgctgg gcaagtaaag cgaaataacg ctcaacctgc cgaaatcgca acgcacaaac
    37921 cgcccttccc tgtcgaattc ttcaatgccc ataccgattt gcacattgtc gggtttgcgt
    37981 ttgctgtaca ccgccacgcc gctgtaaccg cgcttctcgg cgcaatgcca atggccgtgc
    38041 atcccgtgcg gatttttcat atcggcagac aaatcagcct cctgcgcttt gagttcctgc
    38101 acgcagacaa tgtccgcgcc cgatgcggcg atgtattcgt aaaaaccttt tttgtaggcg
    38161 gagcggatgc cgttgacgtt ggcggaaatg attttaagca taataaaaat aagttctcac
    38221 aatgttccca tacacaaagc agcacccggt gcgccgcccg gcatttccca ttcggccctg
    38281 cctgctgaaa acggcggggc ttcaagcggg aaatgccgtc tgaaaacgga aaaagagcaa
    38341 aaggcggtac ttttgccctt tccgatacag ttcaaccgcg ccgccaagtc gtgccgccgg
    38401 cgttgtcttc caaaatgatt ttgtgttcgt tcagaaggtc gcggatgcgg tcggattccg
    38461 cccagttttt atcggcgcgc gcctgtttgc ggcgggcgat caagtcttcg atttcttcgt
    38521 tggagaggcc gtctgaagcc gcgctgcctt gcaggaactc ggtcgggtcg cgttgcagca
    38581 ggccgatgat gccgcccaag gctttcagac ggcctgccag ttgcgcgtca ttggttttgt
    38641 tcacttcgcc ggcaagttcg aacaacaccg ccaccgcttt caccgtatca aaatcatcat
    38701 tcatcgcaac ataaaagcgg cgcgtgtagt catcgccggc ttcagacggc atcggatcgg
    38761 cgggcggcgt atttttcaaa gtcgtataca aacgcgtcaa cgcgcctttt gcatcatcca
    38821 aatgcgcgtc ggaatagttc aacgggctgc ggtagtgggc gcgcaggatg aagaagcgca
    38881 cgacttccgg atcgtattgt ttcaacactt cgcggatggt gaagaagttg cccagcgatt
    38941 tggacatctt ttcgccgtcc acgcggataa agccgttgtg cagccagtat ttgacgtggc
    39001 tggcgatgct ttgcccgtgg tgggtttgcg cgtgatgatg accgcaggta tgccccgtcg
    39061 cgccgacgct ttgggcaatt tcgttttcgt ggtgcggaaa ctgcaaatcc gcgccgccgc
    39121 cgtgaatgtc gaacgtatcg ccgaacaggt tttcactcat cgcagaacat tcgatgtgcc
    39181 agcccggccg tcccctgccc cacgggcttt cccatgccgg ctcgcctgct ttcgccgctt
    39241 tccacaacac aaaatcaagc ggatcgcgtt tgaaaccgtc cacttccaca cgttcgcccg
    39301 cgcgcaggtc gtccaacgat ttgcccgaca attggccgta agcggcaaac tcgcgcacgg
    39361 cgtagtaaac gtcgccattt gcggcaggat atgccttgcc gttttgaatc agggtttcaa
    39421 tcatggcaat catttgtggg atgttttccg tcgccttcgg ctcgatatcg ggacgcaaca
    39481 cacccaaagc atcggcatct tcgtgcatag cctgaatgaa acgcgcagtc agctcgccga
    39541 tagtctcgcc gttttcagcc gcgcgggcaa tgattttgtc gtcgatgtcg gtgatgttgc
    39601 gcacataagt gagcggatag ccgcactcgc gcaaccaacg ggcaatcatg tcgaacacaa
    39661 ccatcacacg ggcatggcct aagtggcaat agtcgtaaac agtcatgccg caaacgtaca
    39721 tacgcacgtt ttcagggtcg atgggggtaa agggttcttt ttggcgggta agggtgttgt
    39781 agatggtggt catgggatta tggattaatc tttgttgctc ggatgataat ttctgttctg
    39841 ttcctgtaga tacggaccaa ggaacattac gcagttgcgg attattaata tggctgatat
    39901 ttgtgaaaat tggttctgca taacagtttg caaaattttt tgtaaattct gataatttaa
    39961 acttatcttt taataagttt gctaaatctg atgacgaggg ataaagttta cttcttatat
    40021 taggcatttc aatatgaagg actattttta tttcgttaca atctaaagcc aagcgagaaa
    40081 aatctttttc ttcctgtttt tctgctttaa atttagcaga aaccaatcct gccaatgaat
    40141 ctcgaatttt tcttgcgatt aaatatggta ggtcagaaag tttttcatct attgattggg
    40201 catttggctc aagcccaagt cggtaataat ctttaatttc gattagccaa agtgtcgact
    40261 catgaagggc tattatatct acacctgagc tgccattatc gtcatctaca ctttgattta
    40321 tcccgttctt tcccttttca tttgtatcaa ttttattacg taaattacaa ctgttctgaa
    40381 aaattttata atgttcccat tcgtcatact tggtaacgta ataatcttca ggaaaagcaa
    40441 aggttaatct cttttctgtg attgtagtca tagcttaacc tcaaatattc agatacctgt
    40501 ctgcctgcat aatgttttca tctaacaata tcaatgtgtt caaatcatta atactgttcc
    40561 cttgctccac ttttgttcca tcatcggaag caatcaacga gaaaaaacgt acaggtaaat
    40621 ccgtgttatt ttcaagcttc aaaagttcca attctctcaa taagaataaa gagtgtgttg
    40681 caataaaaac ctgaataccc tgttgagata aagaccaaat aatacgggca gccactttga
    40741 tcaatttagg attcagatta gcttccggtt catcccaaaa tagatagcct ttatccagca
    40801 atgcccctgt tgcgattaac cgggcaatca tgacaaattt ccgcaaaccc tctgctacca
    40861 aaggtgcttc aatcttaccg cccgtatttg tcagcgatag ataaaacctt ccttgttctt
    40921 cagatacttt tccgcccatc gcgttctcaa taggttcgag caattctcga atttttgttt
    40981 ctctggggcc tttggcaagc gggtgattta attgcataca ggcatcaaac caagtttctt
    41041 cgaaagggat gctttggttt tgatacaaag aagtgaacca agggcaaagt gtaattaatt
    41101 cgcggctggg taagaagata ggtgtcggag tatattcatt ttctttcaat ccgatgcttt
    41161 gaacattgac ttgcgatgat gagttactgg aaaaattcag actactatgc gtagtgccgt
    41221 tttgcagttt taaaacgatt tccgtacgcc cgcgcccctg caaacgtttg ctcaacctgc
    41281 ccaaggaatc gggacggaaa acattcagta atttatcggc aaaacttttt tgcaattctg
    41341 ttttcagtaa tctgtttttg gtgttagatg ttacttctag caggctgtat aaaattttta
    41401 acaaatgtgt tttgccacaa ccgttttcgg caacaataac attgagattt tcagaaaatt
    41461 caaaagtatc gtttggaaga acggtaaagt ttgtcaactc aagcgactgg atatattggt
    41521 tagatgacat ttttaatcca tttcaatctt gctttaaaat tgtttcaaac aaccttttgt
    41581 agaacaaata tcgtctgaaa ccctttcttt tttcactccg gcttaaacac gcccgtatcc
    41641 gttttaggct gctgttcgat aatttcaaca tttgccgccg ctttctccgc ttctgctttt
    41701 tcggcttcca agcgtttttt ctcggtcagg tattggttga tttggtgtac caattcctgc
    41761 gtgccttggt gggttagcgc gctgatttgg aagaggcgcg gggtttccat atcgaattgg
    41821 aagcggtcgt ccggtttcgg gtaatcccag ccgacggctt cgaggaaggt gacggtgcgt
    41881 tcttgggctt cttcttcatc gagcatgtcg agtttgttca gcaccagcca gcgcggtttg
    41941 ccatagagtt cttcatcgta tttgcgtaat tcgttgatga tggcaagggc ttcttcggcg
    42001 ggatcgacgg cttcgtcgaa gggcgccaaa tcgacgacgt gcagcaataa tccggtgcgc
    42061 gataagtgtt tgaggaaacg atgccccaaa cccgcgcctt ctgccgcgcc ttcaatcagg
    42121 ccggggatgt cggccatcac gaagctgtgg ttttcgtcga tgcgtaccac gcccaagttc
    42181 ggatgcaggg tggtgaaggg gtagttggcg attttggggc gtgcggcgga tacggcggta
    42241 atcagggtgg atttgccggc gttgggcatg cctaataagc cgacatcggc gaggactttg
    42301 agttcgagtt gcagggagcg ggtttcgcct tcttcgccgg gcgtggattg ttttggggcg
    42361 cggttgacgg aggatttgaa gtggatgttg cccaagccgc ctttgccgcc tttggctagg
    42421 cagacgcgct gtccgtggta agtgaggtcg gcaacgattt cgtcggtgtc gaggtcgcgg
    42481 ataagggtgc cgacgggcat tttgaggacg atgtcgtccg cacctgcgcc gtaacggtcg
    42541 gaaccgtggc ctttttcgcc gtttttggct tggtagcgtt taacgaagcg gtattcgacg
    42601 agggtgttgg tgttttcgtc ggcttccgcc cagacgctac cgcctttgcc gccgtcgccg
    42661 ccgtccgggc cgccgcgcgg tacgaatttt tcgcggcgga aactggttgc gccattaccg
    42721 cctttgcctg cggcgacttc gatttttgct tcgtcgatga atttcattca atgctcttgt
    42781 ttgttggttt caaatggggg gttcagacgg attaccatgt gttttgatgc cgtccgaaca
    42841 gaatttcgga cgctattata agggataagc ggtatttcaa cacgccgtac ccaaactatt
    42901 tgttccgccc atcttaatga atttttaagc aaatcttcag cctgcaaaca aaattatgtc
    42961 caacttcttt ggtacaatcg cgcctttttg acattccgac ccgacggaat gtccgttcaa
    43021 accgttacat ataataagtt ttggggctgt cctagataac taggataaac tcggttttac
    43081 taattgtttt aaaatggaac aagaactttt atctcactgt tgttaaaacg ccattcgcac
    43141 tcctttaaat acagctcaaa atgcgctttg ggaatgccgt taaacttgcg taaatgacgt
    43201 tttgcttgat tccaaaagtt ctcaattcca ttaatatggt tttgtcgttc ggcaaaatgt
    43261 gtgctgtgat tgatacgaaa acgaagtttc agcgaagcta aaatggctaa attcgctcac
    43321 atctaataca tcatagctac gat
//
LOCUS       DAWFIE010000006        40898 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWFIE010000006 DAWFIE010000000
VERSION     DAWFIE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40898
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.006"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(305..997)
                     /locus_tag="WGQ71_000294"
     CDS             complement(305..997)
                     /locus_tag="WGQ71_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0706366.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(1105..2298)
                     /locus_tag="WGQ71_000295"
     CDS             complement(1105..2298)
                     /locus_tag="WGQ71_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0706367.1"
                     /translation="MSDQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2427..3164)
                     /gene="pxpA"
                     /locus_tag="WGQ71_000296"
     CDS             complement(2427..3164)
                     /gene="pxpA"
                     /locus_tag="WGQ71_000296"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ0706368.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3151..4080)
                     /locus_tag="WGQ71_000297"
     CDS             complement(3151..4080)
                     /locus_tag="WGQ71_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ0706369.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQVYLNQIRRITHEAG"
     gene            complement(4077..4724)
                     /gene="pxpB"
                     /locus_tag="WGQ71_000298"
     CDS             complement(4077..4724)
                     /gene="pxpB"
                     /locus_tag="WGQ71_000298"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ0706370.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4908..7115
                     /gene="uvrD"
                     /locus_tag="WGQ71_000299"
     CDS             4908..7115
                     /gene="uvrD"
                     /locus_tag="WGQ71_000299"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ0706371.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVNVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKIAAFVRLIEALRNQVGQLPLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKPAYDSYGNTRQTATFKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            7472..8011
                     /locus_tag="WGQ71_000300"
     CDS             7472..8011
                     /locus_tag="WGQ71_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0706372.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYQEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAENHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHALFALDYRACGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(8201..8416)
                     /locus_tag="WGQ71_000301"
     CDS             complement(8201..8416)
                     /locus_tag="WGQ71_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706373.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(8424..8627)
                     /locus_tag="WGQ71_000302"
     CDS             complement(8424..8627)
                     /locus_tag="WGQ71_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706374.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8540..8899)
                     /locus_tag="WGQ71_000303"
     CDS             complement(8540..8899)
                     /locus_tag="WGQ71_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0706375.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            8952..9329
                     /locus_tag="WGQ71_000304"
     CDS             8952..9329
                     /locus_tag="WGQ71_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0706376.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWSQAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVQISILKQLVKSSLS"
     gene            9528..10148
                     /locus_tag="WGQ71_000305"
     CDS             9528..10148
                     /locus_tag="WGQ71_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ0706377.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            10171..11622
                     /locus_tag="WGQ71_000306"
     CDS             10171..11622
                     /locus_tag="WGQ71_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ0706378.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFSNVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            12073..12429
                     /locus_tag="WGQ71_000307"
     CDS             12073..12429
                     /locus_tag="WGQ71_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ0706379.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGVLEWE"
     gene            12420..12902
                     /locus_tag="WGQ71_000308"
     CDS             12420..12902
                     /locus_tag="WGQ71_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ0706380.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            12915..13508
                     /locus_tag="WGQ71_000309"
     CDS             12915..13508
                     /locus_tag="WGQ71_000309"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ0706381.1"
                     /translation="MASIQNLYETVVGVLGDQASKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVPVYNSADWYEREAFDLYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            13498..14754
                     /gene="nuoD"
                     /locus_tag="WGQ71_000310"
     CDS             13498..14754
                     /gene="nuoD"
                     /locus_tag="WGQ71_000310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ0706382.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVTPERAMQKGFTGVMLRGSGVEWDVRKKQPYEMYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            14754..15227
                     /gene="nuoE"
                     /locus_tag="WGQ71_000311"
     CDS             14754..15227
                     /gene="nuoE"
                     /locus_tag="WGQ71_000311"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ0706383.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            15617..16918
                     /gene="nuoF"
                     /locus_tag="WGQ71_000312"
     CDS             15617..16918
                     /gene="nuoF"
                     /locus_tag="WGQ71_000312"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ0706384.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVSFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            16918..17292
                     /locus_tag="WGQ71_000313"
     CDS             16918..17292
                     /locus_tag="WGQ71_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706385.1"
                     /translation="MAEKNYFGYPCDDWEAAKLEAKKYLIEKVAKLGNDITYGNLALA
                     IKTIDIPAYGDAMSNFLDEMSRSTYDAHGFMLSALVVSQKTGEPGKGFWILAKELNGE
                     QKSWGYWYRNEKKKIWEKYKQS"
     gene            17503..17814
                     /locus_tag="WGQ71_000314"
     CDS             17503..17814
                     /locus_tag="WGQ71_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0706386.1"
                     /translation="MQNHPFASQPAVYILANGKNGTLYIGVTMNLPERVWQHKNHVNI
                     DGFTARYDVHDLVWYEFFENMPEAVAKEKTMKKWRREWKIKLIEEQNPEWLDLSGVLF
                     V"
     gene            18096..20357
                     /locus_tag="WGQ71_000315"
     CDS             18096..20357
                     /locus_tag="WGQ71_000315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ0706387.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAVQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTVAI
                     LKDALDAESLPSKLNNRAASTQKDFQTASSRLVRVGGVGIYHTDAIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            20360..21436
                     /gene="nuoH"
                     /locus_tag="WGQ71_000316"
     CDS             20360..21436
                     /gene="nuoH"
                     /locus_tag="WGQ71_000316"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ0706388.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPRLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILISALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            21517..21996
                     /gene="nuoI"
                     /locus_tag="WGQ71_000317"
     CDS             21517..21996
                     /gene="nuoI"
                     /locus_tag="WGQ71_000317"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ0706389.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            22008..22679
                     /locus_tag="WGQ71_000318"
     CDS             22008..22679
                     /locus_tag="WGQ71_000318"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ0706390.1"
                     /translation="MTFSTILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTNLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            22676..22981
                     /gene="nuoK"
                     /locus_tag="WGQ71_000319"
     CDS             22676..22981
                     /gene="nuoK"
                     /locus_tag="WGQ71_000319"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ0706391.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLMMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTIDVADLDGLKG"
     gene            23015..23590
                     /locus_tag="WGQ71_000320"
     CDS             23015..23590
                     /locus_tag="WGQ71_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine monophosphate-protein transferase
                     NmFic"
                     /protein_id="HEZ0706392.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            23714..24055
                     /locus_tag="WGQ71_000321"
     CDS             23714..24055
                     /locus_tag="WGQ71_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706393.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            24104..26128
                     /gene="nuoL"
                     /locus_tag="WGQ71_000322"
     CDS             24104..26128
                     /gene="nuoL"
                     /locus_tag="WGQ71_000322"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ0706394.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPVTWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLVVTLPLILLAVPSVIIGYIAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSARLVGAVAAQVRKVQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            26184..26504
                     /locus_tag="WGQ71_000323"
     CDS             26184..26504
                     /locus_tag="WGQ71_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEZ0706395.1"
                     /translation="MSNTQTIRSADFTTSRAWGALDIANMNGTTVRLHWTDQPYKWHV
                     NDGEEVFAVMDGEVDMHYRENGEEHIVRLKSGDIFYAGIGAEHVAHPRGETRILVIEK
                     EGSV"
     gene            26579..28294
                     /locus_tag="WGQ71_000324"
     CDS             26579..28294
                     /locus_tag="WGQ71_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEZ0706396.1"
                     /translation="MANLNVSSKKIINLLGNQDMQEKYFIIPEYQRPYRWDTEKCAVL
                     WSDIVDFRQSSTGKNEEYFLGTIVVCPNEKGNLEVIDGQQRLTSLLLLLRAFYRVLEQ
                     TESTPDTAAKITTLKSKIAPCIWDVDVLTAEITDRTAIRLHTQVATETDNEILHEILA
                     NGEISEECRSNYAVNYLYFLKQCQEYARTNPMDWYHLCLTILNDCILLPIESDSLNSA
                     LTIFSTLNDRGLPLSDADIFKARLYKMAQQKDKFVEQWKELVALAEGASISVDDLFRY
                     YTHVLRAKEKITDKEIGLRRFYTDNRNKQLENENLFNHLIELAGFWQDINNSKTNQDV
                     ETSNFVNDEAAKWLQCLNVYPNEFWKYAASVFFIANKGAQSFKDDFAALLKQLTAFLY
                     AKFLVAPTVTAIKDDIYKLCVAIYHGEKNALSYVMPAPIHYEQNTGRITKGLLMINAY
                     LFDEKQKLLPTKTEIEHIFPKKWQETNYNGWSIIDAEQYLEQLGNKIVFEKRLNIQAG
                     NGYFGKKKEQYQKSDIVEVRELAGENANDWLPSDIQKRQQQIVDRLKQFFDNCFEAAS
                     IPKKN"
     gene            28331..29827
                     /locus_tag="WGQ71_000325"
     CDS             28331..29827
                     /locus_tag="WGQ71_000325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ0706397.1"
                     /translation="MFSNHLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKM"
     gene            29837..31282
                     /gene="nuoN"
                     /locus_tag="WGQ71_000326"
     CDS             29837..31282
                     /gene="nuoN"
                     /locus_tag="WGQ71_000326"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ0706398.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVIMSLIGAFYYLRVVKVMYFDESGRAHPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            31394..31693
                     /locus_tag="WGQ71_000327"
     CDS             31394..31693
                     /locus_tag="WGQ71_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ0706399.1"
                     /translation="MTASMYILLLLALIFANAPFLTTKLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(31802..32698)
                     /locus_tag="WGQ71_000328"
     CDS             complement(31802..32698)
                     /locus_tag="WGQ71_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ0706400.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGVVHEAVEQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHVQYDEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGATACPDLSDAELSVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(32682..32906)
                     /locus_tag="WGQ71_000329"
     CDS             complement(32682..32906)
                     /locus_tag="WGQ71_000329"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ0706401.1"
                     /translation="MKKNAPKSFEEALSRLESLTQAMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGLKELNLESDE"
     gene            complement(32967..33143)
                     /locus_tag="WGQ71_000330"
     CDS             complement(32967..33143)
                     /locus_tag="WGQ71_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII"
                     /protein_id="HEZ0706402.1"
                     /translation="MPSETGFGFQTASVFQKCYNPLFTGTSETMFQHAGRHLKHRPMC
                     RPDLEGLTPLPNKV"
     gene            complement(33170..34093)
                     /gene="rsgA"
                     /locus_tag="WGQ71_000331"
     CDS             complement(33170..34093)
                     /gene="rsgA"
                     /locus_tag="WGQ71_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ0706403.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENADILRPVLQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETRAASPERLAFLQGITDELPG"
     gene            complement(34098..35954)
                     /locus_tag="WGQ71_000332"
     CDS             complement(34098..35954)
                     /locus_tag="WGQ71_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0706404.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESAQLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVSQGEIKFEHVDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(35980..36117)
                     /locus_tag="WGQ71_000333"
     CDS             complement(35980..36117)
                     /locus_tag="WGQ71_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706405.1"
                     /translation="MPSEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(36117..36701)
                     /gene="ruvA"
                     /locus_tag="WGQ71_000334"
     CDS             complement(36117..36701)
                     /gene="ruvA"
                     /locus_tag="WGQ71_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ0706406.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            complement(36783..37109)
                     /locus_tag="WGQ71_000335"
     CDS             complement(36783..37109)
                     /locus_tag="WGQ71_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0706407.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(37163..37882)
                     /locus_tag="WGQ71_000336"
     CDS             complement(37163..37882)
                     /locus_tag="WGQ71_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ0706408.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGKSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASTSSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQAHGMVQTVLTAG"
     gene            complement(37879..38001)
                     /locus_tag="WGQ71_000337"
     CDS             complement(37879..38001)
                     /locus_tag="WGQ71_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0706409.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            complement(38012..38476)
                     /gene="trmL"
                     /locus_tag="WGQ71_000338"
     CDS             complement(38012..38476)
                     /gene="trmL"
                     /locus_tag="WGQ71_000338"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ0706410.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(38541..39038)
                     /locus_tag="WGQ71_000339"
     CDS             complement(38541..39038)
                     /locus_tag="WGQ71_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0706411.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     GERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKLGANRICCWTLAR
                     TPMKK"
     gene            39094..40008
                     /gene="bioH"
                     /locus_tag="WGQ71_000340"
     CDS             39094..40008
                     /gene="bioH"
                     /locus_tag="WGQ71_000340"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ0706412.1"
                     /translation="MCLKHRGQLFAASVRYAAKSLRQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQQERKSMPDAVKKVYLIHGWGANRHMFDDLMPRLPATWPVSAVDLPGHGDA
                     PFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TADEDYPEGLAAPALGKMVGAFRSDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            40010..40798
                     /locus_tag="WGQ71_000341"
     CDS             40010..40798
                     /locus_tag="WGQ71_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0706413.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKYILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFTGKGVVQHCQSPIAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWACALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGIWRAMAWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKR"
BASE COUNT         9466 a        10369 c        10928 g        10135 t
ORIGIN      
        1 gtggtatcgt tcgccttggg tcagttgcgt gtggctcatg gcaatctttc ttgcaggaaa
       61 ggccgtatgc taccgcatac tggccttttt ctgttaggga aagttgcact tcaaatgcga
      121 atccgccgac ctctttcagt tacagcagct tgatcccttt cccttatcca acggggggaa
      181 ggctaggata gggtggcttg caaatataca gaacaaggga caagagccac cctctctcca
      241 accctctccc tccgtacggg agggggtgga ttctcgcggg cgaagcccac gctacggtta
      301 gccttcaccc cagcacaaac aattcccgcc cgtgcgcctt cagccaactt ttagcattgt
      361 cggtatgcgg cgtcagcgtg ttcaccaaat gccaaaagcg cggactgtgg tcggggtggc
      421 ggaggtggca gagttcgtgg atgcagacat agtcggcgac gtattcgggc gtgccgatca
      481 gccgccagtt gaggcggatg ccggtgtgcg ggcggcatac gccccaaaag gttttggcgt
      541 tgctcaggtc tgtggcggtg ggcgtcagtc ctgtttcggc tgcgtgtttt tcaaggcggg
      601 gcagcaggta ttcgcgggcg cgttcgttca acaggcggcg caggtggtcg atttgtgcgg
      661 cggtttcttt tcggggaagc aggatttcag acgacgtgat acggatatgg ctttggctgt
      721 gggtatccag cttggtcttt attccccgat accaaatcca ctcgggtaag tttgggtggg
      781 aaacaggatg cacgggcgtt ttggcaagcg tgttccgcaa aatcgtttcg tttgccgcca
      841 gccagtttgc taacgcgtgg tcttgaaaaa agggtgggac gttgatgctg accgtctgca
      901 tattgacggg gcgcagaatc agatttttct tggcactgcg tttgagttcg atttcgatgc
      961 acaaaccgtc ggaaagagta taggtgaagc gtttcatagt tgtgaatagg tttcagaccg
     1021 gatacatcgt ctgaaacagg aattttccat atcaggcggc aaacttcgga taatatacaa
     1081 aatcaaacat ctgcgctaca aggttcagcc gaacaagccg ccgatatatt tgctgatggt
     1141 aatggcgctg agtactgcca tcaaaccgac cacaatcacg ccggaaacgg tgagccacag
     1201 cgggtgtttg tagtcgccga caattttggt tttgtaggcg gcaatcagaa tcagaccgag
     1261 ggaaatcggt aaaatcaggc cgtttaatgc gcctacgaac accagcacct gcgccggttt
     1321 gccgatggtg gaaaatacgg cggtggacac ggcgataaag gcaataatcc atttgttttt
     1381 attgcgttcg atagacgggc tgagaccgga gaagaacgac accgaagtat aagccgcacc
     1441 aatcaccgaa gtaatcgaag ccgcccaaat caccacgccg aaaatcagca ggccgatgta
     1501 tcccgccgca tattcaaacg gtgtggaagc agggttgtcg ggattgagct gtacgccttg
     1561 gctgaccacg cccaaaaccg ccaaaaacaa tacaatccgc ataatcgagg caatcagaat
     1621 cgcccgcacc gagctttggc tcacttccgg caacgccgat ttgcctttga tgcctgcgtc
     1681 cagcagacgg tgcgcaccgg cgaaggtgat gtagccgccg accgtgccgc ccaccagtgt
     1741 aacaatcgcc attgcatcga gtttttccgg cataaaggta tgcacggcgg catctgccag
     1801 cggcggattc gcctgccatg ccacataaac cgtcagcgca atcattacga aacccatcac
     1861 ttgggcgaat ttgtccatca ctttgcctgc ttctttaaac agaaacacac cgatggcaat
     1921 cacgccgctg atcacggcac cggtttccgg cgacagtccg gtcagcaggt tcagacccaa
     1981 gcctgcgccg ccgacgttgc caatattgaa cgccaaaccg cccatcacaa tcagcacagc
     2041 caagaaatag cctgcgccgg gcaagacctg attggcaata tcctgcgcct gtttttcgga
     2101 aacggcgaca atccgccaaa tattgagctg cgccccgatg tcgagcagaa tcgagagcag
     2161 aatcacaaag ccgaaacttg ccgccagtgc ttgggtgaag gtggcggttt gggtcagaaa
     2221 gcccgggccg atggcggaag tcgccatcag gaatgcagcg ccgattaagg catttctgcg
     2281 gtttttttga tcagacataa tcgcttatcc tctataaaat tggttgttgc tgtgtttggg
     2341 cgaaacctgc ggttttagct acgcagaaac tcgctttgct cgttttggcg aaacctgcgg
     2401 ttttcagacg gcctatgaac tgtttttcaa gcagaaactt tgatgcctgc cgccagtagt
     2461 tcctgccgga ttttttcggc aaacaccacg gcgtgcggcc cgtctccgtg cagacagatg
     2521 ctgtcggctt gcacggcaac caggctgccg ttcactgctt tgacccgccc gtcccgcacc
     2581 atctgcaata cttgggcgat ggcttcttcg tcgctgtcca cctgcgcatc ggggcggctg
     2641 cggggaacca gcgtaccgtc gggcatatag cggcggtcgg cgaatacttc ggaaatcaca
     2701 cccaagcctg cggcttttcc ggcttccaag agcaggctgc cggaaagtgc catcaatttc
     2761 aatttcgggt cgaaatccgc cacaattcgg gcaacggtat ccgccagcgc acggtttttc
     2821 gccgcttggt tgtacattgc gccgtgcggt ttgacataag ccatttccaa accctgatca
     2881 cggcacaagg cctgcaatgc gcccaactgg taattcagac acgcccgcaa atcggcttcg
     2941 gacagattca tttcggtacg gccgaagttt tcccgatcgg gatagccggg gtgtgctccg
     3001 atgcgcacgc cgttttgttg ggcatacgcc aatgccgccc gaatatcggc aatgctgccg
     3061 gcgtgttggg cgcaggcgat gttggccgaa gtaatcagct gcaacaaggc ttcgtcgctg
     3121 ccgcagcctt cggcgagatc ggcgtttaaa tcaacctgct tcatgggtga ttctccgtat
     3181 ttggttcaga tagacttggt ttttgcgccg cagggcggtg gcttctttca agccgattat
     3241 tttgaatttg actttgctgc cgaagcgcac ctgtgccagc ctgcccaaat cggcggcggc
     3301 aacggtagcg attttcggat aaccgccggt ggtttgcgca tcggccagca ggataatcgg
     3361 tttgccgccg ggcggcacct gcacggttcc tgcctgaaca gcgtgggaca gcatttccaa
     3421 aggttgcgac agggtcagcg gctgtccgtc gaagcggtag cccatgcggt tgctatcgct
     3481 ttgcagcgtc cacgtttccc gttccagatt cagacgccct ttttcactga aagcggcata
     3541 ttccgacgaa ggaacaaggt ggacggtatc ggtaaacggt atcggggcaa tgccgacttt
     3601 ggacaattcc tgcgcacctt tgccgatggg gagacaatca cctttttgca gcattctgcc
     3661 ttgatggccg ccgaaaccgg ctttcaggtc ggtgcttctc gaacccatca cttccggcac
     3721 atcaaatccg cccgccacgc acacatagcc gtacatgccc tgcacggcac gcaccatttt
     3781 caaggtctgc cctttgcggg cggtataacg ccaatacgaa tagaccggtt cgccgtccaa
     3841 ttccgcctga tacacggcac cggtgagaca aaacggcgta tcccgttcaa acaccagcat
     3901 tatcccgccc aaagcgattt cgattgcggc cgtgccttcg tcgttgccca ataaaatatt
     3961 acccgccgcc aaagcaaccg tgtccatcgc accggcatga ccgatgccgt aacgccggtg
     4021 tccgtagcgt ccggtatcct gaatatgcgc cggtgcctgc actgccgaaa cgtgaatcat
     4081 ggctcaatcc tttctgcaac aaagcggact tggtcacccg ccgccagcag ggtcggcgga
     4141 ttcaaatcgg ctcggaacaa gggtaattcg gttctgccga taatctgcca gccgccgggc
     4201 gaagcgaacg gatacacacc ggtctgactg ccgccgatac cgaccgaacc ggcaggaacg
     4261 gacgttctcg gcacggcacg gcggggcgtg tgcaatgctt cgggcaagcc gcccagataa
     4321 gggaaaccag gctggaagcc catcataaat acggtataag tttgcgccgt atggcggcgg
     4381 acgatttcgg aaataaccgt ctgatggaaa gcagcgactt ccgccaaatc cgggccgtat
     4441 tcgccgccgt agcagacggg aatttccacc agtttgccct gatggtctgt aacggcggtg
     4501 tgttcccaca catattgcaa ttcatcggca agcgtcgcca aatcggtatc gaaacgggtg
     4561 aacacggtca gattgttcat gccgaccacc acttcctcaa tcctgtcgtg ctgcccgagc
     4621 gcagcggcaa acgcccacaa cttttgctgt ttgcccagtt cggaaggtgc attcagtcgg
     4681 cagaccaaag cggattcgct gattggtgtg atctctattc tcatttgttg ttcattttgg
     4741 ttatgtttta atgaatctat atgcaggggc ggcggtttgt caatatcttc tgtgctgcat
     4801 catcaaaccg tcgattggaa aagtgctgcc ctgccgctgc actttttcag acgaccttaa
     4861 accgtttcta ttaaaatagc gcattccact tttcagacgg catctctatg tttcccgacc
     4921 aatccgcccc caacctgctg caaggcttga accctgaaca actttccgcc gtaacctggc
     4981 cgccgcaatc cgcccttgtg ctggcaggcg cgggcagcgg caaaacgcgc gtgctgacca
     5041 cgcgtatcgc atggctgttg caaaccggtc aggcaagcgt gcatagcatt atggcggtaa
     5101 cgtttaccaa taaagccgcc aaagaaatgc agacccgttt gggcgcaatg attcccgtca
     5161 acgtccgcgc catgtggctc ggcacgtttc acggtctctg ccaccgcttt ttgcgcctgc
     5221 accaccgcga cgcaggcctg ccgtcttcct ttcaaattct cgacagcggc gaccagcttt
     5281 ccctcatcaa acgcctgctc aaaagcctca acatcgccga agaaatcatc gcaccgcgtt
     5341 cgctgcaagg ctttatcaac gcgcaaaaag aatccggttt gcgcgcttcc gtgttgagcg
     5401 cgcccgaccc gcacacaagc cgcatgatcg aatgctacgc cgaatacgac aaaatctgcc
     5461 aacgcgaagg cgtggtcgat tttgccgaac tcatgctccg cagctacgaa atgctgcaaa
     5521 gcaacgaaat cctgcgccag cattaccaaa accgcttcaa ccacattctc gtcgacgagt
     5581 tccaagacac caacaaactg caatacgcct ggctcaaact catggcgggc ggaaacgcag
     5641 cggtatttgc cgtcggcgac gacgaccaaa gcatttaccg tttccgtggc gcaaacgttg
     5701 gcaatatgac cgcgctgatg gaagaattcc acatcgacgc gcccgtcaaa ctcgaacaaa
     5761 actaccgctc cgtcggcaac atccttgccg ccgccaacgc tgttatcgaa aacaacgacg
     5821 aacgcctcgg caaaaacctg cgcaccgacg ccgaagcagg cgacaaaatt cgctactact
     5881 ccgcctttac cgacctcgaa gaagcccggt tcatcgtgga cgaaaccaaa gccctcgaac
     5941 gcgaaggctg ggatttggac gaaatcgccg tcctctaccg cagcaacgcc caatcgcgcg
     6001 tcatcgaaca aagcctgttc cgcagcggca tcccctacaa aatctacggc ggcttgcgct
     6061 tttacgaacg ccaagaaatc aaacacgcgc tcgcctacct acgcctcgcc gtcaatcccg
     6121 acgacgacaa cgccctcttg cgtgtcatca acttcccacc gcgcggcatc ggcgcacgca
     6181 ccgtcgagaa ccttcagacg gcctcaaacg aacaaggcat caccctctgg caagccgcct
     6241 gcaacgccgg cgcgaaagcc gccaaaatcg ccgccttcgt ccgcctgatt gaagcactgc
     6301 gcaaccaagt cggacaactg cccctgtccg aaatcatcgt cggcatcctc aaagacagcg
     6361 gcctgaccga acactaccga acccaaaaag gcgacaacca agaccgcctc gacaaccttg
     6421 acgaactcgt caacgccgcc atcgagttca aacccgaaga cagcaacttt gaaaccctgc
     6481 ccgaaaacat ttcagacgac cccgccttcc ccatcctcgc cttcctcagc aacgccgccc
     6541 tcgaatccgg cgaaaaccaa gcaggcgcag gtgaaaaagc cgtccaactc atgaccgtcc
     6601 acgccgccaa aggcttggaa ttcaacgccg tcttcctcac cggcatggaa gaaggccgct
     6661 tccccagcga aatgagcctt gccgaacgcg gcggcctcga agaagaacgc cgcctcatgt
     6721 acgtcgccat cacccgcgcc cgcaaacgcc tctacatcac catggcacag caacgcatgc
     6781 tgcacggaca aacccaattc ggcatcgtct cccgcttcgt cgaagaaatc ccacccgaag
     6841 tattgcacta cctgtccgtc aaaaagcctg cctacgacag ttacggcaac acgcgtcaaa
     6901 ctgccacgtt caaagacaaa atcatcgacg actacaaaca gccgcaaacc tacgcaggct
     6961 tccgtatcgg acaaaacgtc cgccacgcca aattcggcac cggcgtgatt atcgatgccg
     7021 cagataaagg cgaatccgcc cgactgacca tcaacttcgg caaacagggc gtgaaagagt
     7081 tggataccaa gtttgcgaaa ttggaagcga tgtaaatttg aaatgcaggc tggatactcg
     7141 tatccgacaa aaatatttga cgcgtctatc gtttccgaaa aaccgctgtt ggaaaatgtc
     7201 ggattcgaga atccgaccta tggcaaaaaa ctgtagcaag gacaaggcaa aaggccgtct
     7261 gaatccggta acaacaaatt ttgccgaaac ctccgccgtc attcccgcgc aggcgggaat
     7321 ccagaccttt cggcacggaa acttatcgga taaaaggttt ctttagattc cacgttctag
     7381 attcccgcct gcgcgggaat gacggacggt aaaagccggg tatagatacc taacccttgt
     7441 ttatagctga aaatcaataa ggaagaacat tatgtcccaa gtttttaaag attttgattt
     7501 gtcctccgta tgggaatccg acagttgggc agatgaaaac taccaagaag ccccgtttac
     7561 ccctgaaatt ttggctgccg tagaaagtga actgggctat aaattgccgc aaagttttat
     7621 tgaattgatg gcggtacaaa acggcggaat atttgtcaaa aactgttttc cgaccacaca
     7681 gagaaattcg tgggcggaaa atcatgtgca aatttgcgag gtatcgggca tcggctttga
     7741 aaaagaaggg agtttgtgcg gcgaaacagg gcaaaaactt tggctggaag aatgggaata
     7801 tccgcccatc ggcgtgtatt ttgccaacga cccgtcaggc ggtcatgccc tgtttgcctt
     7861 ggactatcgg gcgtgcggca aagacggcga gccgaaagtg gtgtttgtcg agcaagaatc
     7921 ggattttgaa atcgtcgaac ttgcccccga ttttgaaacc tttatccgca gcttgcggca
     7981 tgaagatgag tttattgacg aggaaatata aaacggcggt tgaaaaaccg gaatcatcaa
     8041 gggaaaacgg gcggaataac agataaccga ttgaatccgt aaggaaaacg gtttggtgga
     8101 acgcgccatc caagaccttt gcaaagaaaa actgtctccg acagcattga cattattaac
     8161 agaacttatc aagtttggag ctatctcaaa tataattcgg ttatcctttt gtatccatta
     8221 aatcatatgc ttcaattaat tgttgttcta gctcttgtac caattttgaa ttgcgaattc
     8281 ctgacacaat ctcaaattct tctgcatcta tgcaaacacc tgcataaatt tcaataacaa
     8341 gggaacgcaa taattgaagc tcttctcttg ttaaagaaat aataatgtca tcacctgtgt
     8401 aattgattat attcataata attttatttt tgtttgtcaa agtaagtttt gcctaaggtt
     8461 ggtctaaatg cagttccacc atcttttgaa tttgggtctc tgattacaat tgctccagac
     8521 ttatcatccc aaattgctct tatgtgtttg gattgtaatc ttcgaattcc caagaaaaaa
     8581 atcgtaataa gtttgaaagt gtcaaatccc aagtttcttt tgagcaatat tctaatattt
     8641 tatcaatttc acttttaata atcttatgat caaactgttc taatattaat gcattagacc
     8701 aaaaaaaacc ttctttatta caatgatggg aaatccattt aggagaacaa atgcaaagtg
     8761 aaaaaataga tgagccttgt tctccttcga ttccgatatc caaatctatc catctatgga
     8821 aattatctgg aatttcgggg gtaaattttt caaaatcaat atcatataaa tttatgcttt
     8881 ttaaatccaa tttaatcatt agggctgtcc tagataaata gggaaattca aattaagtta
     8941 gaattatccc tatgagaaaa agtcgtctaa gccggtataa acaaaataaa ctcattgagc
     9001 tatttgtcgc aggtgtaact gcaagaacag caacagagcc cgacagcatt gtttatacgg
     9061 attgttatcg tagctatgat gtattagatg tgagcgaatt tagccatttc cgtatcaatc
     9121 acagcacaca ttttgctgaa cgacaaaacc atatcaatgg aattgagaat ttttggagcc
     9181 aggcaaaacg tcatttacgc aagtttaacg gcattcccaa agcgcatttt gagctgtatt
     9241 taaaggagtg cgaatggcgt tttaacaaca gtgagataaa agttcaaatt tccattttaa
     9301 aacaattagt aaaatcgagt ttatcttagt tgtccaggac agccccatta tttttataac
     9361 accgtgaagc cgcacagcag tttgaacagt gatacgccgt ttgcgggctt acgagtttat
     9421 tttcccggcc tgcagtttga gcaatacggt gatttcctac ggttaataca aatgtttaca
     9481 cattgataca tttcatttat agttccgcct atttgaaaat agaaaatatg aattcgaccg
     9541 caagtaaaac cctgaaagga ttgtcgctgg tgtttttcgc ctctggattc tgcgccctga
     9601 tttaccaggt cagctggcag aggcttctat tcagtcacat aggtatcgat ttgagttcga
     9661 ttactgtcat tatttctgta tttatggtcg gcttgggtgt aggtgcgtat ttcggtggac
     9721 gcattgctga ccgttttcct tcaagtatca tccccctgtt ttgcatcgct gaagtatcca
     9781 tcggtctgtt cggtttggta agcaggggtc tgatttccgg cttggggcat cttttagttg
     9841 aggctgattt gcccatcatc gctgctgcca atttcctctt attgctgctt cctaccttta
     9901 tgatgggcgc gaccttgccc ttgctgacct gtttttttaa ccggaaaata cataatgttg
     9961 gcgagtctat cggtacctta tattttttca acactttggg tgcggcactc ggatcgcttg
    10021 ccgccgccga atttttctac gtctttttta ccctctccca aaccattgcg ctgacagcct
    10081 gctttaacct tctgattgct gcttcagtat ggctgcgtta cagaaaggat ggatatagtg
    10141 aacactaaac cgaatactag tttgatttat atgctttctt tccttagcgg cttattgagc
    10201 ttgggtatag aagtcttgtg ggtaaggatg ttttcgttcg cagcacagtc cgtgcctcag
    10261 gcattttcat ttacccttgc ctgttttctg accggtatcg ccgtcggcgc gtattttggc
    10321 aaacggattt gccgcagccg ctttgttgat attcccttta tcgggcagtg cttcttgtgg
    10381 gcgggtattg ccgacttttt gattttgggt gctgcgtggt tgttgacggg tttttccggc
    10441 ttcgtccacc acgccggtat cttcattacc ctgtctgccg tcgtcagagg gttgattttc
    10501 ccgctcgtac accatgtggg tacggatggc aacaaatccg gacgacaggt ttccaatgtt
    10561 tatttctcca acgttgccgg cagtgcattg ggtccggtcc ttatcggctt tgtgatactt
    10621 gatttcttgt ccacccaaca gatttacctg ctcatctgtt tgatttctgc tgctgtccct
    10681 ttgttttgta cactgttcca aaaaagtctc cgactgaatg cagtgtcggt agcagtttcc
    10741 ctaatgttcg gcatcctcat gttcctactg ccggattctg tctttcaaaa tattgctgac
    10801 cgtccggata ggctgattga aaacaaacac ggcattgttg cggtttacca tagagatggt
    10861 gataaggttg tttatggggc gaatgtatac gacggcgcat acaataccga tgtattcaat
    10921 agtgtcaacg gcatcgaacg tgcctatctg ctaccctccc tgaagtctgg catacgccgc
    10981 attttcgtcg ttggattgag tacaggttcg tgggcgcgcg tcttgtctgc cattccggaa
    11041 atgcagtcga tgatcgttgc ggaaatcaat ccggcatacc gtagccttat cgcggatgag
    11101 ccgcaaatcg ccccgctttt gcaggacaaa cgtgttgaaa ttgtattgga tgacggtagg
    11161 aaatggctgc gtcgccatcc tgatgaaaaa ttcgacctga ttttgatgaa tacgacttgg
    11221 tactggcgtg cctattccac caacctgttg agtgcggaat ttttaaaaca ggtgcaaagc
    11281 caccttaccc cggatggtat tgtaatgttt aataccacgc acagcccgca tgcttttgct
    11341 accgccgtac acagtattcc ctatgcatac cgctatgggc atatggtagt cggctcggca
    11401 accccggtag ttttccctaa taaagaactg ctcaagcaac gtctctcccg gttgatttgg
    11461 ccggaaagcg gcaggcacgt atttgacagc agcaccgtgg atgctgcagc acaaaaggtt
    11521 gtctctcgta tgctgattca gatgacggaa ccttcggctg gggcggaagt cattaccgac
    11581 gataatatga ttgtagaata caaatacggc agagggattt aaccgtctta aagggtttca
    11641 ggcaacgcag gttttaggta acgtcctgct agttcaaaaa aaccgcatca cagcagtcgg
    11701 gacaaaatgg tttaaacatt ttgtcccgaa ttcttattcc tatatatagt ggattaacaa
    11761 aaatcaggac aaagcgacga agccgcagac agtacaaata gtacggaacc gatttcagca
    11821 ccttagagaa tcgttctctt tgagctaagg cgaggcaacg ccgtactggt ttttgttaat
    11881 ccactatacc acgaattacg gtgtaaaaat ttatatgacc ttataaaatc aaataagaat
    11941 cgttatcata acatgattgt atttattggg tttttttggg cgttttgccg atatttacct
    12001 tttaatggtt tttgaaattc gctaaaatac gaaattattg tagaaatttt gttaacggat
    12061 ttgggtgtaa ccatgttgtc cgcttacttt cccgtctttg tctttatcct cgtcggcctc
    12121 gcggccggcg tgctgtttat cctgctcggc acgattttag gcccgaaacg ccactacgcc
    12181 gaaaaagacg cgccttacga atgcggtttt gaagcctttg aaaacgccag gatgaagttc
    12241 gacgtgcgct attacctcgt cgccatcctc tttatcttgt ttgatttgga ggtcgcgttc
    12301 atgttgccgt gggcagtcgt gttcaaagat ttgggcgcgt acggcttctg gtctatgctg
    12361 gtgtttatcg tcgttctgac ggtaggcttt gtttacgaat ggaaaaaagg tgtgctggaa
    12421 tgggaataga aggcgttttg aaaaaaggtt tcatcaccac cagcgcggat acggtgttga
    12481 actatatgcg taccggttcg ttgtggccgg ttactttcgg cttggcctgc tgcgccgtgg
    12541 aaatgatgca cgcgggtatg gcgcgttatg accttgaccg tttcggcatc attttccgtc
    12601 cgtccccacg tcaggccgac ctgatgattg tggcgggtac gctgaccaac aaaatggcac
    12661 ccgccctgcg ccgcgtgtat gaccagctcg ccgagccgcg ctgggtattg tctatgggct
    12721 cgtgtgccaa cggcggcggc tattatcact attcttattc cgttgtgcgc ggtgccgacc
    12781 gcgtcgtgcc ggtagacgtt tatgtgccgg gttgtccgcc gactgcggaa gccctgattt
    12841 acggcctgat tcagctccaa caaaaaatca agcgcacttc caccatcgcg cgtgacgagt
    12901 aaggagagga cgatatggca agcattcaaa acttatacga gaccgttgtc ggcgtgcttg
    12961 gcgatcaggc aagcaaagtc atttctgctt tgggcgagat taccgttgag tgtctgcccg
    13021 aacactatat ttcagtcatg accgcattgc gcgaccatga agagttgcat ttcgagcttc
    13081 tggtcgactt gtgcggtgtc gattacagca cttacaaaaa cgaagtatgg cagggcaaac
    13141 gctttgccgt cgtcagtcag ttgctttccg ttaaaaacaa tcaacgcatc cgcgtacgcg
    13201 tctgggtttc agacgacgac ttccccgtag tcgaatccgt agttcccgtt tacaacagcg
    13261 cggattggta cgagcgcgaa gccttcgatt tgtacggcat catgttcaac aaccatcccg
    13321 acttgcgccg catcctgacc gattacggct ttgtcggaca tccgttccgc aaagacttcc
    13381 cgatttccgg ctatgtggaa atgcgttacg acgaagagca aaaacgcgtg atttaccaac
    13441 ctgttaccat tgagccgcgc gagatcacgc cgcgtatcgt ccgtgaggag aactacggtg
    13501 gccaataaat taagaaacta caccatcaac ttcggcccgc aacaccctgc ggcgcacggc
    13561 gtattgcgta tgattttgga gttggaaggc gaaaccatcg tccgtgccga cccgcatatc
    13621 ggtctcctgc atcgcggcac cgaaaaactg gcggaaacca aaacctatct gcaagccctg
    13681 ccttatatgg acaggctgga ctacgtttcc atgatggtca acgagcaggc atactgcttg
    13741 gcggtagaaa aactcgccgg aattgatgta cctatccgcg cccaatacat ccgcgtgatg
    13801 ttcgccgaag taacgcgcat cctcaatcac ttgatgggca tcggttcgca cgcattcgac
    13861 atcggcgcga tgaccgccat cctctacgcc ttccgcgacc gcgaagagct gatggacttg
    13921 tacgaagccg tgtccggcgc gcgtatgcac gccgcctact tccgtcccgg cggcgtttac
    13981 cgcgacctgc ccgacttcat gcccaaatac gagagcagca aattccgcaa tgccaaagta
    14041 ttgaagcagc tcaacgaatc ccgcgaaggc accatgctcg actttatcga tgccttctgc
    14101 gaacgcttcc ccaaaaatat cgacacactc gaaaccctcc tgaccgacaa ccgtatttgg
    14161 aaacagcgta ccgtcggcat cggcgttgtc actcctgagc gcgccatgca aaaaggcttt
    14221 accggcgtga tgttgcgcgg ttcgggtgtg gaatgggacg tgcgtaagaa acagccttac
    14281 gaaatgtacg acaaaatgga tttcgacatc cctgtcggcg tgaacggcga ctgctacgac
    14341 cgctacctct gccgtatgga agaaatgcgt caatccgtac gcatcatcaa acaatgttcc
    14401 gagtggttgc gtgtcaatcc gggtccggtc attaccacaa accacaaatt cgctccgccc
    14461 aaacgtaccg aaatgaaaac aggtatggaa gacctgattc accatttcaa actctttacc
    14521 gagggtatgc acgttcccga gggcgagacc tacaccgctg tcgaacaccc gaaaggcgag
    14581 ttcggcgttt acatcatttc agacggcgcg aacaaaccct accgcctgaa aatccgcgca
    14641 cccggcttcg cccatctgca aggcatggac gaaatggcaa aaggccacat gctcgccgac
    14701 gtcgttgcca tcatcggtac gcaggacatc gtattcgggg aggttgaccg ataatgttat
    14761 ccgcagagtc cttaaaacaa atcgacacag aactggcgaa atatcccgcc gaccaacgcc
    14821 gttccgccat catgggtgcg ttgcgtatcg cccaaaccga aaaaggctgg ctcgcccccg
    14881 aaaccatcgc ttttgttgcc gactatatcg gcatcacgcc tgcacaagcc tacgaagtcg
    14941 ccactttcta caatatgtac gaccttgagc ctgtcggcaa atacaaactg accgtttgta
    15001 ccaacctgcc ctgcgccctg cgcggcggta tggctaccgg cgaatacctc aaacaaaaac
    15061 tcggtatcgg ctacggcgaa actacccctg acggcaagtt tacccttgtc gaaggcgaat
    15121 gcatgggcgc atgcggtgat gcccccgtta tgctggtcaa caaccacagc atgtgcagct
    15181 ttatgaccga agaagcgatt gagaagaaac tggcggagtt ggagtaggtc gtctgaaacg
    15241 acgatttaaa cgtaggtcgg atacttgtat ccgacagagt gggtaaaaag gcaaaatgtc
    15301 ggatttaaga atccgcccta ctgaaatacc gaaatgccgt cattcccgcg caggcgggaa
    15361 tccaccggta agattcggtt tctgaattta ataagacatt gcttaccatt gaggatggat
    15421 tctcgcctgc gcgggaatga cgacagacaa gcaagtggtc gagatccaac aaaaacgatt
    15481 aaaggtcgtc tgaaaatatc gatttgataa actagatttt atttcagacg acgttacaag
    15541 ccggtacaca aagacatctt aaggtcgtct gaaacagcgg ccgcaaccga tacgaaaaca
    15601 aacaggcaca ccaaaaatgg ctatttacca atcaggcgtg atttttgacc aagtggatac
    15661 cgccaatccc gattgctgga cattggacga atacgtcaaa cgtggcggtt ataccgccct
    15721 gcgtaaaatc ctgtccgaaa acatctcgca aaccgatgtg attgacgaag tcaaaacctc
    15781 cggcttgcgc ggtcgtggcg gtgcgggctt cccgaccggt ttgaaatgga gctttatgcc
    15841 ccgttctttc cccggcgaaa agtacgtcgt ttgcaacacc gacgaaggcg agccgggtac
    15901 gtttaaagac cgcgacatta ttatgttcaa cccccatgcc ctgattgaag gcatgattat
    15961 cgccggttac gcgatgggcg caaaagcggg ctacaactat atccacggcg aaatttttga
    16021 aggctaccaa cgctttgagg ccgctttgga gcaggcgcgt gccgcaggct ttttgggtaa
    16081 aaatattttg ggttcggatt ttgaattcga actcttcgcc caccacggct acggtgcata
    16141 catctgcggc gaagaaaccg cattgcttga atcgctggaa ggcaaaaaag gccagccgcg
    16201 ctttaaaccg ccattccctg cttcgttcgg cctgtacggc aaaccgacta ccatcaacaa
    16261 taccgaaact ttctcctccg tttcattcat tatccgtgac ggcggacagg catttgccga
    16321 taaaggtatt ccgaatgcag gcggtaccaa attattctgt atttccggcc atgtcgagcg
    16381 tccgggcaac tatgaagtgc cattgggcac accgtttgcc gaagtcttga aaatggcagg
    16441 cggtatgcgc ggcggcaaaa aactcaaagc cgtcattccc ggcggttcgt ccgcgcccgt
    16501 attgcctgcc gacatcatga tgcagacaaa tatggactac gactcgattt ccaaagcagg
    16561 ctcgatgctg ggttctggcg cgattatcgt catggacgaa gacgtgtgca tggtgaaagc
    16621 ccttgagcgc ctgagctact tctactacga cgagtcttgc ggccaatgta cgccttgccg
    16681 agaaggcacc ggctggcttt accgcatcgt ccaccgcatc gtagaaggca aaggtaaaat
    16741 ggaagatttg gatttgctgg attctgtcgg caaccaaatg gcaggccgca ccatctgcgc
    16801 ccttgccgat gccgccgtct tccccgtccg aagctttacc aagcatttcc gtgatgagtt
    16861 tgtgcattac atcgaacacg gcggaccgat gaaagagcat aagtggggag ggtggtaatg
    16921 gcagaaaaga attattttgg ctacccttgt gatgattggg aagcagctaa actagaagca
    16981 aagaaatatt tgattgagaa agttgcgaaa ctaggcaatg acatcactta tggaaatttg
    17041 gcattggcta ttaaaacaat tgatattcct gcatacggtg atgccatgag caatttcctt
    17101 gatgagatga gtcggtcaac atatgatgca cacggtttta tgttaagtgc tttggtggtc
    17161 tcacagaaaa ctggggaacc tggtaaaggg ttttggatat tggcaaaaga attaaatgga
    17221 gaacagaaaa gctgggggta ttggtaccgc aatgagaaaa agaaaatttg ggaaaaatat
    17281 aaacaatcgt aaggtcgtct gaaaaacaca tcgccgtcat tcccgcgtag gcgggaatcc
    17341 atagtggaat ttacagaacc cgatatttga aaagcagttg ccaaaatcca aaaatggatt
    17401 cccgcttacg cgggaatgac ggcgggagtg ggcagatgtt ttcagatgaa aacggctgta
    17461 aatgatattg aaaaaagttg tttatattgc aggaaacatt aaatgcaaaa ccatccgttt
    17521 gctagtcaac ctgccgtata tattttggca aacggtaaaa acggaacact ctatatcggc
    17581 gttaccatga atttgccgga aagagtttgg cagcacaaaa accatgtcaa tattgatggc
    17641 tttactgccc gatatgatgt gcatgatttg gtttggtatg agttttttga gaatatgcct
    17701 gaagcagttg ccaaagaaaa aacgatgaaa aaatggcgac gtgaatggaa gattaaattg
    17761 attgaagaac aaaatcccga atggttggac ttgtcgggcg tgttgtttgt ttagttttat
    17821 ttctggaact ttaaaaactg tcgttattcc agccccacct acgcgcagac aggctacggc
    17881 gggaatcacc gcaaaagtta agaaaccaat gtttgaaaac agttaccgaa aacccaagaa
    17941 tggattcccg cctgtgcggg aatgacggca aggtggcagt aaacgtttta aacagtattg
    18001 attgtcaatg aaactcaaaa ggccgtctga aacccatttt tcagacgacc tccataaaag
    18061 attatttatc aaatacccgt aactaggaac gaaccatgtt acaaatcgaa atcgacggca
    18121 aacaggtatc tgtggagcag ggtgcgacgg tgattgaagc cgcgcacaag ctcggtactt
    18181 atatcccgca tttctgttac cacaaaaaac tttccatcgc cgccaactgc cgtatgtgtt
    18241 tggtggacgt ggaaaaagct cccaaacctc tgcccgcctg tgccacgccg gtaacggacg
    18301 gcatgattgt gcgtacgcat tcggcaaaag cccgagaggc gcaagaaggt gtgatggagt
    18361 tcctgctcat caaccatccg cttgattgtc cgacctgcga ccaaggcggc gaatgccagt
    18421 tgcaggattt ggcggtgggc tacggtaaaa ccaccagccg ctacaccgaa gaaaaacgtt
    18481 ccgtcgtcgg caaagacatg gggcctttgg tttccgccga ggaaatgagc cgctgcatcc
    18541 actgtacccg ttgcgtccgt ttcactgaag aaatcgccgg cttgcaggaa attgcgatgg
    18601 tgaatcgcgg cgagcattct gagattatgc cctttatcgg caaagcggtg gaaaccgaat
    18661 tgtcgggcaa cgtcatcgat ttgtgtcccg tcggcgcgct gaccagcaaa ccgttccgct
    18721 tcaacgcgcg tacttgggaa ttgaatcgcc gcaaatccgt ttccgctcat gacgctttgg
    18781 gcagtaacct gattgtacaa accaaagacc ataccgtccg ccgcgtgttg ccgctggaaa
    18841 acgaagcgat taacgaatgc tggctgtccg accgcgaccg ttttgcctac gaaggcctgt
    18901 atcacgaaag ccgtctgaaa aacccgaaaa tcaaacaggg cggcgagtgg atggacgtgg
    18961 attggaaaac cgcattggaa tatgtccgca gcgcgattga atgtatcgcc aaagacggca
    19021 accaaaacca agtcggcatt tgggcgaacc cgatgaatac ggttgaagag ctgtatctgg
    19081 ccaaaaaact tgccgacggc ttgggtgtta aaaactttgc aacccgtttg cgtcaacaag
    19141 acaaacgtct ttcagacggc attaagggtg cgcaatggtt gggacaaagc attgaatctt
    19201 tggctgacaa cgatgccgta ttggtagtcg gcgcgaactt gcgcaaagaa cagccgctcc
    19261 tgaccgcccg cctgcgccgc gccgccaaag accgtatggc attgagcgta ttggccggca
    19321 gcaaggaaga attgtttatg ccgcttctct ctcaggaagc cgcacatccc gacgagtggg
    19381 caggccgtct gaaaaacttg tctgccaatg cggaacacgc cgttaccgcc agcctgaaaa
    19441 acgctgaaaa agcagcggtg attttgggcg cggaagtgca aaaccatcct gattacgccg
    19501 cgatttatgc cgccgttcaa gagctggcag atgcgaccgg cgcagtgctg ggtattttgc
    19561 cgcaagccgc caacagcgtt ggtgcggatg tcttgggcgt gaactcgggc gagagcgttg
    19621 ccgaaatggc aaacgcgccg aaacaggcag tcttgctgct caacgtcgag cctgaaatcg
    19681 atacggttga cggtgcaaaa gccgtggctg cgttgaaaca agcgaaaagc gtgatggcgt
    19741 ttacgccgtt tgtcagcgaa acgctgctgg acgtgtgcga cgtattgttg ccgattgcgc
    19801 cgtttaccga aacctcgggc agcttcatca atatggaagg ccgtctgcaa tctttccacg
    19861 gcgtggtaca aggcttcggc gattcgcgtc cgatgtggaa agtgttgcgc gtattgggca
    19921 acctgtttga cctgaaaggt tttgaatacc acgataccgt cgcgattctg aaagacgcgc
    19981 tggatgcgga aagcctgccg tccaaactga acaaccgcgc cgcttcgact caaaaagatt
    20041 ttcagacggc atcaagccgc ctcgtgcgcg tgggcggagt gggcatctat cacaccgatg
    20101 ctatcgtgcg ccgttccgca ccgttgcaag aaaccagcca tgccgccgtg cctgctgcgc
    20161 gtgtaaatcc aaatacattg gcacgcttgg gcctgcaaga cggacaaacc gctgtcgcca
    20221 aacaaaacgg cgcaagcgta tcggttgccg tcaaagccga tgccggtttg cctgaaaacg
    20281 tggtgcattt gccgctgcat accgaaaatg ccgcgctggg tgcgttgatg gacactattg
    20341 aactggcggg agcttgatca tgcaagaatg gttccaaaac ctctttgccg caacgctcgg
    20401 tctgggcgat ttgggtatta ctgtaggctt ggtggtatcc gtcatcgtca aaattgtgat
    20461 tatcctgatt ccgctgattc tgaccgtcgc ctacctgact tattttgaac gtaaagtcat
    20521 cggcttcatg cagcttcgcg tcggtccgaa cgtaaccggt ccgtggggtc tgattcagcc
    20581 gtttgccgac gtgttcaaac tcttgtttaa agaagtaacc cgtccgaggc tgtcaaacaa
    20641 agccctgttc tacattggtc cgattatgtc gctcgccccg tcttttgcgg cgtgggcggt
    20701 gattccgttt aacgaagaat gggtgctgac caacatcaat atcggtcttt tgtacatcct
    20761 gatgattacc tcgctgtcgg tttacggcgt gatcatcgcg ggctgggctt ccaactccaa
    20821 atattcgttt ttgggcgcaa tgcgtgcttc cgcacaaagc atttcctacg agattgccat
    20881 gagtgccgcg ctggtgtgtg tcgtgatggt atcgggcagc atgaacttct ccgacatcgt
    20941 tgccgcacaa gccaaaggta ttgccggcgg ttcggtattc tcttggaact ggctgccgct
    21001 cttccccatc tttatcgtct atctgatttc cgccgttgcc gaaaccaacc gtgcgccgtt
    21061 tgacgtggca gagggtgagt ctgaaatcgt tgccggacac cacgtcgaat actccggctt
    21121 cgcattcgca ctgttcttcc ttgctgaata cattttcatg attctgattt ccgcgctgac
    21181 atcgttgatg ttcctcggtg gttggttgtc tccgttccca caaagctggg gctttatcgg
    21241 cacgccttcc gcattctgga tgttcgtgaa aatggcggcg gttctgtact ggtatctgtg
    21301 gatccgtgca accttcccgc gctaccgtta cgaccaaatc atgcgtttgg gctggaaagt
    21361 gctgattccg atcggcttcg cctacatcgt ggttttgggc gtgtggatga tttcaccgct
    21421 gaatttgtgg aaataagttt cagacggcat cttgaggccg tctgaacaaa gcgattttga
    21481 atacctaacg aaatccctgt tttgagggaa cataatatgg ctaacttagt aaaaaccttt
    21541 ctgcttggcg aattggtaaa aggtatgggc gtaacgctca aaaacttttt cgcccgcaaa
    21601 gacacgattt attttcctga agagaaaacg ccgcaatccg tgcgtttccg cggcctgcac
    21661 gcgcagcggc ggtatccgaa cggtgaggaa cgctgcattg cgtgtaagtt gtgcgaggcg
    21721 gtgtgtccgg caatggcgat caatatcgaa tcggaagaac gcgaagacgg cacccgccgc
    21781 accaagcgtt acgacatcga cctgaccaag tgcatcttct gcggtttctg cgaagaggct
    21841 tgcccgaccg atgcgattgt ggaaacgcac atttttgaat accacggcga gaaaaaaggc
    21901 gacttgcaca tgaccaagcc gattcttttg gccattggcg acaaatacga agctgaaatc
    21961 gccaaacgca aagccgctga cgcgccgtat cgttaaggag cagacgaatg actttttcca
    22021 cgattctgtt ctatatcctt gccgccatcg ttttgtacgg tgcggttcgt accgtcaccg
    22081 ctaaaaaccc tgtccatgcc gctttgcatc tggtgctgac cttctgcgtg agcgcgatga
    22141 tttggatgct gatgcaggcg gagtttttgg gcgtgacgct ggtggtggtt tacgtcggcg
    22201 ccgtgatggt gttgttcctg ttcgtcgtga tgatgttgaa tatcgacatt gaagaaatgc
    22261 gcgccggttt ttggcgtcat gcgcctgttg ccggtgtggt cggcacattg ttggcggttg
    22321 ctctgatcct gattctggtc aatccgaaaa ccaacctggc cgcatttggt ctgatgaaag
    22381 acattccggc cgattacaac aatatccgcg atttgggcag ccgtatttat accgactacc
    22441 tgttgccgtt tgaattggcg gcggtattgc tgctgttggg tatggtggcc gcgattgcac
    22501 tggttcaccg caaaacggtt aatccgaaac gtatggatcc tgcagaccaa gtcaaagtac
    22561 gcgccgatca aggccgtatg cgtctggtga aaatggaagc ggtcaaaccg caagtcgaat
    22621 ctgccgaaga aagcgaagtt tcagacggcc tcaagccgga aggggaggga aaagcatgat
    22681 taccttgacg cattatctgg tattgggtgc gctcctgttc ggtatcagcg caatgggtat
    22741 cttcatgaac cgtaaaaacg tgctggtatt gatgatgtcc atcgagctga tgcttttggc
    22801 ggtgaacttc aactttatcg ccttctcaca acatttgggc gatactgccg gacaaatttt
    22861 cgtattcttc gtattgaccg ttgccgctgc cgaatctgcc atcggtttgg cgattatggt
    22921 gctggtgtac cgcaaccgac aaacgattga tgttgccgat ttggatgggt tgaaagggta
    22981 aaggtaggtt gggtcgagac ctgacaagac accgatgccg tctgaaaacc cgataggaaa
    23041 aacgatgaaa tccatagacg aacaaagcct gcataatgcc cgccgcctgt ttgaaagcgg
    23101 cgacatcgac cgtatcgaag tcggtaccac cgcgggcctg caacagattc accgttacct
    23161 gttcggcggc ttatatgatt ttgcgggtca aatcagggaa gacaacattt ccaaaggcgg
    23221 ttttcgtttt gccaacgcca tgtatttaaa agaggctttg gttaaaatcg agcagatgcc
    23281 cgagcggact tttgaagaaa tcatcgccaa atatgttgaa atgaacattg cccatccgtt
    23341 tttggagggt aatggcagaa gtacccgcat ctggctggat ttggtgctga aaaaaaacct
    23401 gaaaaaagtc gtgaactggc aaaatgtaag taaaaccctg tatttgcagg cgatggaacg
    23461 cagccccgtc aacgatttag aactgcgctt tctgttaaag gacaacctga ctgacgatgt
    23521 ggacaaccgt gaaatcatct ttaaaggtat cgagcagtcg tattattacg aagggtatga
    23581 aaaaggctga aggtcgtctg aaaagcgatt tcagattgtt tcagacgacc tgatttggta
    23641 ggtgatcaga cgggagcgga tgagaaaaga aattctgggt aagaataatc cggtctgaaa
    23701 tattggaaga agaatgatgg ataaaaatca gttagaacaa gaatttcata aagccatgtt
    23761 aaatatttat caggaggctt tgaatttgcc gcaaccttac aaggcgacac gatttttaca
    23821 aattgtaaat gaatttggtg gtaaagaggc ggcggataaa ttattgagta cgggggaaaa
    23881 gaagactcag accggtttta cagagctgat tttgagtggt ggcggagtcc acgccttgaa
    23941 atacagtatg gaatatctgg tgttacaaaa gccgtggtgt gatttattta ctgaagagca
    24001 attagctgtg gcacgcaaac gattggagcg tgttggattt gtttttccga agtaattttg
    24061 tacgaaacaa acatagattt ttaaatcaat cggattcaat caaatgaacg acatgacttt
    24121 atatctcatc attgccctcg tgcctttggc aggctcgctg attgcgggtt tgttcggcaa
    24181 caaaattggt cgtgccggtg cgcatacggt tacgatactc ggcgtggctg tgtccgccgt
    24241 gctgtcggct tatgtgctgt ggggctttat cgacggcagc cgcgccaagt ttgacgagaa
    24301 tgtctatacc tggctgacga tgggcggttt ggatttctcc gtcggcttct tggtcgatac
    24361 gatgacggcg atgatgatgg tcgtggtcac cggcgtatcg ctgatggtgc atatctatac
    24421 catcggttat atgcacgatg agaaagtcgg ctaccaacgc ttcttcagct acatttcttt
    24481 gtttactttc agtatgttga tgctgattat gagcaacaac ttcatccagc tcttcttcgg
    24541 ctgggaagct gtgggcttgg tgtcgtatct cttgatcggt ttctatttca aacgtccgag
    24601 cgcaacattt gccaacctga aagccttttt gatcaaccgt gtcggcgact tcggcttttt
    24661 gctcggtatc ggcttggtgc ttgcttattt tggcggcagc ctgcgctatc aggacgtatt
    24721 cgcttatctg cccaacgtcc aaaatgccac catccagctc ttccccggtg tggaatggtc
    24781 tttgattacc gtaacctgtt tgctcttgtt tgtgggtgcg atgggtaaat cggcacaatt
    24841 cccgctgcac gtctggctgc ctgattcgat ggaaggcccg accccgattt ccgcattgat
    24901 tcacgctgcg acgatggtta cggcgggtct gtttatggta tcgcgtatgt cgccgattta
    24961 cgaaatgagc agcactgcgc tgtccgttat catggtgatc ggtgcgatta ccgccctgtt
    25021 tatgggcttc ttgggcgtga ttcaaaacga catcaaacgt gtggttgcgt attccaccct
    25081 gtcgcaactg ggctatatga ccgtggcttt gggcgcgtct gcctattccg tggcgatgtt
    25141 ccatgtgatg acccacgcct tctttaaagc cctgttgttc ttggcggcag gcagcgcgat
    25201 tatcggtatg caccacgacc aagacatgcg ccatatgggc aatctgaaaa aatatatgcc
    25261 ggttacttgg ctgaccatgc tgatcggtaa cttgtcgctg attggtacgc cgttcttctc
    25321 cggcttctac tccaaagatt cgattatcga agcggcgaaa tacagcacac tgccgggcag
    25381 cggctttgcc tattttgccg tcctcgccag cgtgtttgtt accgcgtttt acgcgttccg
    25441 ccaatacttt atggtgttcc acggcgaaga gaaatggcgc agcctgcccg aacaccattc
    25501 agacggccac ggcgaagaac atcacggttt gggtaaaaac gacaatccgc acgaaagccc
    25561 gttggtggtt accctgcctt tgattttgct tgccgttccg tccgtcatca tcggctacat
    25621 cgccatcgaa cccatgcttt acggcgattt cttcaaagac gtgatttttg ttaatgccga
    25681 tgcgcatccg accatgcaca ttatgaaaga agagttccac ggcgcattgg cgatggtgtc
    25741 gcacagcctg cattcgcccg ttctctatct cgctatcgca ggcgtattga gcgcatggct
    25801 tttgtacgtc aaactgccgc acctgcctgc gaaaatcgcg caggcgttcc gtccggttta
    25861 cgttttgttt gaaaacaaat actaccttga tgccctgtat ttcaacgttt tcgccaaagg
    25921 cacacgcgca ttgggtacct tcttctggaa agtcggcgat accgccatta tcgacaacgg
    25981 tattgtcaac ggctctgcca gactggtcgg cgcggttgcc gcgcaagtgc gtaaagtcca
    26041 aacaggcttt atctacacct acgctgccgc tatggtgttc ggcgtattgg tcttgctcgg
    26101 tatgaccttc tggggattgt tcagataaaa atgagatttt cagacgacgt ttaggtcgtc
    26161 tgaaaaaaga aacttgagaa acaatgtcaa acacacaaac catccgctct gccgacttca
    26221 ccacttcccg cgcatggggc gcactcgaca tcgccaatat gaacggcacc accgtgcgcc
    26281 tgcactggac ggaccagccc tacaaatggc acgtcaacga cggcgaggaa gtgtttgccg
    26341 tgatggatgg cgaagtcgat atgcactacc gagaaaacgg cgaagaacac atcgttcggt
    26401 tgaaaagcgg cgacatcttc tacgcaggaa tcggcgcgga acacgtcgcc cacccgcgcg
    26461 gcgaaacgcg gattttagtg attgagaaag aaggcagtgt ttagggcgga tacgcataag
    26521 tattatgtgc tttttcaggt atcctgaaaa tcataaatct atcaacgaga ctttaatcat
    26581 ggcaaatcta aatgtgagta gtaaaaaaat tattaatctg ctaggtaatc aagatatgca
    26641 ggaaaaatat tttatcattc ctgaatatca acgcccctat cgctgggata ccgaaaaatg
    26701 cgccgtatta tggtctgata ttgtggattt tcgccagtcg tccacaggaa aaaatgaaga
    26761 atattttctt ggtacaatcg ttgtttgccc caatgaaaaa ggtaatttgg aagtaattga
    26821 tgggcaacag cgtttaacat cattactgct gttattgcgt gcgttttatc gagttttaga
    26881 gcaaacggaa agtacccccg ataccgcagc caaaattacc acacttaaaa gcaaaattgc
    26941 cccctgtatt tgggatgttg atgttctaac ggctgaaatt accgatagaa ccgcaatccg
    27001 gctgcatacg caagttgcca cagaaacaga taacgaaatt ttgcacgaga ttttggcaaa
    27061 cggtgaaatc tcagaagagt gtcgttctaa ttacgccgtt aattatttgt atttcctgaa
    27121 acagtgccaa gaatacgcca gaaccaaccc aatggattgg tatcatcttt gtttaactat
    27181 tttgaatgac tgtattttac ttccgattga aagtgacagt ttaaatagtg cattaaccat
    27241 attttccaca ttaaacgata ggggcttgcc tttgtcggat gcagatattt ttaaagccag
    27301 gctttataag atggcgcagc agaaagataa atttgttgaa caatggaagg aacttgttgc
    27361 gttggcagag ggtgcatcta tttccgtaga tgatttgttc cgctattaca cccatgtatt
    27421 aagggcgaaa gaaaagatta cggataaaga aattgggctt cgccgttttt atactgacaa
    27481 ccgaaacaaa cagttggaaa acgaaaattt gttcaatcac ttaattgagc tggctggatt
    27541 ttggcaggat attaacaaca gcaaaactaa ccaagacgtg gaaacttcga actttgtaaa
    27601 tgacgaggcg gccaagtggc tgcaatgtct gaatgtctat cctaatgagt tttggaaata
    27661 cgccgcgagt gtgttcttca ttgcaaacaa gggtgcgcaa tcctttaaag atgattttgc
    27721 agcattatta aaacagctta ctgcattttt gtatgctaaa tttttagttg cgccaaccgt
    27781 aactgcaatt aaagatgata tttataaatt atgtgttgcg atttatcacg gtgaaaaaaa
    27841 cgctttatca tatgttatgc ctgcacccat acactatgaa caaaatacag gacgtataac
    27901 taaagggttg ttaatgatta atgcttatct atttgatgaa aagcagaaat tattgccaac
    27961 taaaactgaa atcgaacata tctttccgaa aaaatggcag gaaactaact ataatggttg
    28021 gtcaatcata gatgcggaac aatatttaga acaactaggt aataaaattg tttttgaaaa
    28081 acggttaaat attcaggcag gtaacggcta ttttggtaag aaaaaggagc aatatcaaaa
    28141 atccgatatt gttgaagtgc gtgaattagc aggtgaaaat gccaatgatt ggttaccgag
    28201 tgatattcag aaacgccagc agcagattgt ggatagattg aaacagtttt tcgataattg
    28261 ttttgaagca gccagcatcc ccaaaaaaaa ttaacttttc caataacctc caaaccaaac
    28321 aggtaacgca atgttttcta accatcttct cagcttggca atatggatac ccattgccgc
    28381 aggtgtgctg gttttggcga ccggtaaaga cagccgtgcg ccgctggcgc gtgtgcttgc
    28441 ctttatgggt gcgcttgccg gtttcttggt aacgctgccc ctgtttaccg gtttcgaccg
    28501 tttgagcggc ggctatcaat ttaccgagtt ccacgagtgg attccgctgc tgaaaatcaa
    28561 ctatgcattg ggcgtggacg gtatttcggt gctctttatc atcttgaatg cgtttattac
    28621 gctgttggtg gtattggccg gttgggaagt tattcagaaa cgcccggcgc agtatatggc
    28681 ggcattcctg atgatgtcgg gtttgattaa cggtgcgttt gccgcgcagg atgcgattct
    28741 gttttatgtg ttcttcgagg gtatgctgat tccgctgtac ctgattatcg gtgtatgggg
    28801 cggcccgcgc cgcgtctatg cgtcggtcaa gctgttcctc tatacgctga tgggttcgct
    28861 cctgatgctg gttgctatgg tttacctgta ctatcaaaca ggcagcttct ctattgtcga
    28921 tttccaaaac atcaaacaga ttccgttggg cgtacagcag cttttgtttg cggcattctt
    28981 cctgtcattt gccgtaaaag tgccgatgtt ccccgtacac acttggctgc cggatgccca
    29041 cgttgaagcg ccgaccggcg gttcgatggt gttggcggcg attacgctga aactgggtgc
    29101 gtatggtttc ttgcgcttta tcctgccgat tatgccggac gcggcacgct attttgcccc
    29161 cgtgatcatc gtattgagcc tgattgccgt aatttatatc ggtatggtgg ctttggtgca
    29221 aaccgatatg aaaaaactgg tggcgtattc gtccatcagc catatgggtt ttgtaacgct
    29281 tgggatgttt ttgtttgttg acgggcagtt ggacgactgg gcattgaaag gtgcaatcat
    29341 tcaaatgatt tcccacggtt tcgtgtctgc cgcgatgttt atgtgtatcg gcgtgatgta
    29401 cgaccgcctg catacgcgca atattgccga ttatggcggc gtggtcaatg tgatgcccaa
    29461 gtttgcggcg tttatgatgc tgttcggtat ggcgaacgcc ggtctgcctg cgacttccgg
    29521 ctttgtgggc gagtttatgg tgattatggg cgcggttaaa gtgaatttct gggtaggcgc
    29581 tttggccgcc atgaccctga tttacggtgc gtcttatacc ctgtggatgt acaagcgcgt
    29641 gattttcggc gcgattcaca atccgcacgt tgccgaaatg aaagacatca actgccgcga
    29701 atttgcgatt ctggcggtgt tggcggttgc tgttttgggt atgggcctgt atccgaacgc
    29761 atttatcgaa gtggtgcatc aggcggcaaa cgatttgatt gcccatgtgg cacaaagcaa
    29821 gatgtgaggt gtgtaaatga actggtctga tttgaattta atgcccgcca tgcccgaaat
    29881 cgtgctgctt gcgctgctgg tgttattgtt gctggcggac ttgtgggtca gtgatgacaa
    29941 acgccgttgg acgcattacg gcgcgttggc aacggtggcg gttacggctg tggtgcagct
    30001 tgccgtttgg gaacagggca gcacgtcttc gttcaacggc atgtatattg cagacggtat
    30061 gtcgcgtttg gcaaaaatgg ttttatatgc cttgacgttt gccctgtttg tttatgccaa
    30121 gccctacaac caagtgcgcg gtattttcaa aggcgagttt tacaccctgt cgctgtttgc
    30181 cctcttgggc atgagcgtga tggtgagcgc ggggcatttt ttaactgcct atatcggttt
    30241 ggaactcttg tcgctcgccc tttacgccct gattgccctg cgccgcgatt ccggctttgc
    30301 cgccgaagcc gccttgaaat attttgttct gggcgcgctg gcatccggcc tgctgctcta
    30361 cggtatttct atggtttacg gcgcaaccgg ttcgctggaa tttgccggcg tgctcgcctc
    30421 ttccttcaat gaagaagcaa acgaatggct gttgaaactg ggtctggtgt ttatcgtcgt
    30481 cgccgttgcg ttcaaactcg gcgcggtgcc gttccatatg tgggtgcccg acgtgtatca
    30541 cggcgcgccc acttctgtta ccgccctagt cggcactgcc ccgaaaatcg ccgccgtcgt
    30601 tttcgctttc cgcatcctcg ttaccgggtt gggaaccgtg catcatgact ggtctctgat
    30661 gtttgccctg cttgccgccg cctcgctgct ggtcggcaac cttgccgcca tcatgcagac
    30721 caatatcaaa cgtatgctcg cctattccac cgtatcgcat atggggttta tcctgctggc
    30781 gtttatggcg ggcgcggtcg gctttgcggc ggggctgtat tacgccatta cctacgcgct
    30841 gatggcggcg gcagggttcg gcgtgttgat ggtgttgtcg gacggggaca acgagtgcga
    30901 aaacatcagc gatttggcag ggttgaacca acaccgcgta tggcttgcct ttttgatgct
    30961 gctggttatg ttctccatgg caggcattcc gccgctgatg ggtttttacg ccaaattcgg
    31021 cgtgattatg gcactcttga aacaaggcta tgtttggttg tccgtatttg ccgtcatcat
    31081 gtccctgatt ggcgcgttct actacctgcg cgtggtcaaa gtcatgtatt ttgacgaatc
    31141 cggccgcgcc catcccaccg tcggcggcaa caatgccgca aaattccttt tgagcgtcaa
    31201 cgcgctgctg ttggtgctgt ggggcattat gccgcaaacc gttatcgact ggtgcgccaa
    31261 ggcgttggaa aacacgctgt aagccgccgc aacggcagcc gtgtcagagg ctgccgtttt
    31321 tgttaagata tgccgttccg caacgcagtt cagacggcat cgccgccgac aatgcccaaa
    31381 cagaaagccc gccatgaccg catccatgta catccttttg ctgcttgcct tgatttttgc
    31441 caacgccccc ttcctcacga ccaagctgtt cggcatcgta ccgctcaagc gcaaacattt
    31501 cggacaccac ctgatcgagc tggcggcagg tttcgcgctg accgccgttc ttgcctacat
    31561 cctcgaatcc cgtgcgggag cggtacacga tcagggttgg gagttttacg ccaccgtcgt
    31621 ctgcctgtac ctgatttttg cgtttccctg tttcgtgtgg cggtattttt ggcacacgcg
    31681 caacagggaa taggcaggca taggaatgcc gtctgaaacc tgttcagacg gcatttgttt
    31741 cattcaagtg caggccggca tcgctgtgcc ggcacgtttc agccggcgat atacgccggt
    31801 tttaatattt gcgggcgact gcaaattctg ccaactgccg caggcgcagg gctttgtcgc
    31861 cgaagggttc gagcagcgcg accgcttcgg caaccagttt gtgtgcgtat gagcgcgccg
    31921 cttccaagcc catcagtttc acataagtcg gcttgtcgtt gtctgcgtct ttgcccgccg
    31981 ttttgcccaa agtcgccgtg tccgcttcac aatccaacac atcgtcaatg acttggaacg
    32041 ccagccccag ttttgccgcg taagcgtcca atacggaaag ttccgcatct gacagatcag
    32101 gacacgccgt cgcccccaat aaaaccgccg cacggattag cgcacccgtt ttcaggctgt
    32161 gcatctgttc caaatcggct tgaaccattt gtttgccgac attcgccaaa tcgattgcct
    32221 gaccgcccgc catgcccgcg ctgccgcccg ctttcgccaa caccgacaac attgccaact
    32281 ggcgtgctgc gggcagttct gtcggacggc ttaacacgtc gaaggcttgg gtttgcaaag
    32341 cgtcgccgac cagtagcgcg gtggcttcgt cgtattgcac atggcaagtc ggtttgccgc
    32401 gccgcaggct gtcgttgtcc atcgccggca tatcgtcgtg aaccaaagaa tagacgtgga
    32461 tcatttcgat tgccgccatt gcctgttcta ctgcttcatg cacgaccccg cccaattccg
    32521 aagccgccag caccagcatc ggacgcagac gcttgccgcc gtccaaagcc gcatagcgca
    32581 tcgcttcgtg cagcgtgtgc gggatttcgt tttcagacgg caaaaagcgt tccagcagca
    32641 gctctgtctg tgcctgcgcc ctctgttgcc acgctttcaa atcattcgtc ggattcaagg
    32701 ttcaactcct tcagcccgtc cgcatccaaa acctgtaatt tctgttcgac ttgcgccagc
    32761 ttggtttggc agtatctgac cagctcgttg ccttcctgat aggcggcaag cgcgtcttcc
    32821 aagggcattt cgccctgcat agcttgtgtc agcgattcaa ggcgcgacaa ggcttcttca
    32881 aacgatttcg gggcgttttt cttcatcgtg tttccttttc ggttgaaacc ccgccacttg
    32941 gacatctgtc cttcggggcg gtagaatcag actttatttg ggaggggtgt aagcccttcc
    33001 aaatcaggac gacacatagg gcggtgcttt aagtgtcgtc ctgcgtgttg gaacatagtt
    33061 tcggatgttc cggtaaaaag cggattgtag catttttgaa aaacggatgc cgtctgaaac
    33121 ccgaatccgg tttcagacgg cattgttttg tcctgcaaac ggcaaggcgt tacccgggca
    33181 gttcgtcggt gatgccctgc aaaaaggcga ggcgttcggg gcttgccgcc ctggtttcgg
    33241 cggcggcttt gaaggcgcag ccgggttcgg cgcggtgggt gcagttgtgg aagcggcatt
    33301 gcccgacaag gtggcggaaa tcggggaaat agcgcggcaa atcggcggct tggaggtggt
    33361 gtaaaccaaa ttcttgcaaa cccggggagt cgatgagttg ggtttcgccg ttcaaatcgt
    33421 aaagccgggc atgggtggtg gtgtgttttc ccgagtcgag tgcggcggaa atgtcgccgg
    33481 tgcgggcggt ttggctgcct aaaagggcgt tggtcagggt ggatttgccc ataccgctct
    33541 gcccgagcag gatgttgctg tgcccttgca ggacggggcg caggatgtcg gcgttttcca
    33601 gtgcgcgggt ttcgatgacg ggataaccca gcgtttcgta gaatttgagt ttttcgcgcc
    33661 aaagggcggt ttcgggcagg tcggctttgt tcaggacgat gacggcgcgg atgccggcgg
    33721 cttcggcggc aagcagggcg cgttgcagca gccgcacgct cggactcggg acggcggcgg
    33781 ttacgatgag gagttgggta acgttggcgg cgatgagttt ggttttccac gcgtcttggc
    33841 ggtagagcag gctttggcgc ggtaaaaaat cttcaatcac aacttgttcg gcgttgacgg
    33901 ggctgatgcg gacacggtcg ccgcaggcga aatcgacgcg ttttttgcgc gtgctggctt
    33961 cgtaggttgt gccgtcgggc gtgcggacaa tgtagcggcg gccgtagctg gcggtaattt
    34021 gggcggtgtc gttcatggtt tctttggggt tgggtgtgga aatgccgtct gaaaacgggt
    34081 gttcggacgg catcggttca gtcgtgctgc cactcgacgt gttcgttgag gaagccgccg
    34141 ctctggtgcg cccagagttt ggcgtaaagc ccgcgttttt cgaggagttc ggcgtgtgtg
    34201 ccttcttcga tgatgcggcc tttgtcgagg acgacgagcc tgtccattgc ggcgatggtg
    34261 gagaggcggt gggcgatggc gatgacggtt ttgccgtcca tcattttgtc gaggctttct
    34321 tggatggcgg cttcgacttc ggaatcgagc gcgctggtgg cttcgtccaa aagaagaatc
    34381 ggtgcgtctt tgagcatcac gcgggcgatg gcgatgcgct ggcgttgccc gccggagagt
    34441 ttcacgccgc gttcgccgac gtgtgcgtcg tagccgcgcc gccctttggc atcggaaagg
    34501 tcggggatga agccggcggc ttcggcgcgt tcggcggcag aaaccatttc ggcatcggtc
    34561 gcgtcggggc ggccgtaaat aatgttgtcg cgcacggaac ggtgcagcag cgaggtatct
    34621 tgcgtgacca aaccgatttg ggcgcgtaaa gattcttggg taacgccgct tatgtcctgc
    34681 ccgtcgatcg aaaccgtgcc gctttgcggt tcgtagaagc gcaaaagcag gttgacgatg
    34741 gtggatttgc ccgcgccgct gcgtccgatc aagccgactt tttcgcccgg gcggatggtg
    34801 aggttgaagc cgttgagcag cggtttgcct gcttcgtagc aaaaatcgac gtgttcgaac
    34861 ttgatttcgc cttgcgacac tttcaacggc agggcgttcg gcttgtcgag gatggtgtgc
    34921 ggtttggaca gggttgccat accgtcgccg acggtgccga tgttttcaaa cagctgcgcg
    34981 gattcccaca taatgtattg cgacaaaccg ttgacgcgca acgccatggc ggtggctgta
    35041 gcaaccgcgc ccacgccgac ctgcccgttg tgccagagcc agatgcccag tgcggcggtg
    35101 gagagggtca gggaggtgtt gacgatgaag ctgcacgaat gcagcagcgt cgccagccgc
    35161 atttgggcgc gcaccgtaac cataaattct tccatcgact gcttggcata ggcggcttca
    35221 cgcgcgccgt gggagaagag tttgacggtg gcgatattgg aataggcatc ggtaatgcgg
    35281 ccggtcatca gcgagcgggc atccgcctgc catgcggcgg tttgccccaa tttgggaatc
    35341 agcaggcgca tcaccgaagc gaaaccgaca atccaaccga taaagggcag cagcagccat
    35401 gagtcgagcg aggcgagaat cacgccggag gtaatgaaat acaccgacac ataaacgacc
    35461 atatcggcaa ccgtcatcac cgcgtcgcgc aacgccagcg cggtctgcat gactttggcg
    35521 gacacgcgtc cggcaaattc gtcctgataa aaaccgaggc tttggttcag catcaggcgg
    35581 tggaagttcc agcgcaggcg catagggaac acgccctgaa gggtttgcag gcgcacgttg
    35641 gacgcggcaa acgcccacac gaccgaaaac accatcatcg ccgccattgc cgccagttcc
    35701 caaccttttt cggcaaacag ttcggcgggc gcgtatttgc cgagccactc cacgattttg
    35761 cccataaatt gaaaaatcag ggcttccata atgccgatgc cggcggtcag cgcagccagg
    35821 gcggctatcc atttccgcac gccggccatg ctgctccaga caaaccgcca caagcctttt
    35881 tctggcgttt tcggggcggc ttcgggataa gggtcgattc gggactcgaa ccaggaaaat
    35941 attttgttca acattgtttt cgatttcggt aaaacagttt cagacggcat caaacacaat
    36001 gccgtctgaa aggaaggaca ataacgccat tttacgggaa aagccgtcgg gaagacagcg
    36061 cgaggcggaa acgcagggtt tcgtcagggc aaacgccgcg ccgccttcag acggcattat
    36121 ttcagcaggt ttttcaaagc aaggcgcacg ccttcgccca cgtccgtccc ctccggaacg
    36181 cctttgaccg ccgcttttgc ttcgcgttcg ctgtaaccca gcgcaagcag cgtgctgacg
    36241 atgtcttccg tttcgtcggc ggcgggtgcg gcggcaaaca gcccgtccgt taccgtatgc
    36301 gcgaccagct tgccgcgcag ttccaagacc atacgttcgg cggttttttt gccgattccc
    36361 ggcgcggaag acaggcgttt gacatcttct tctgcaaccg cctgcgccag ttcgtctgcc
    36421 gtcatcgccg acaaaatgcc caaagccgtt ttcgcgccga tgccgccgac cttaatcagt
    36481 tggcggaatg ttttgcgttc ttccgcagtg gcaaaaccaa ataaaagatg tgcgtcttcc
    36541 cgaatgataa gctgggtaaa cagttgtacg ctttcaccca cgggcggcag gttgtagaag
    36601 gtctgcatcg atacgtcggc ctcatagccg acaccgttga catcgatgac gatttgcgga
    36661 gggttttttt caaccagttt gccggtcagt ctgctgatca tgtatgccga atcctgaagt
    36721 gtcgggtgca aaatgccgtc tgaaaccggt tcgggcttca gacggcacgg attgtatcaa
    36781 attcagtcgt cgcggcggga ggaaatcacg cggccggtac gggcatcgac aacgactttg
    36841 tattcctgtc cgtttttgac gatttcgaca tcatagtgcg gacggccgtt gtcgtgttcg
    36901 agatcgatgt cggtgatttt gccgccgaca cgcgccaacg ctgctttttc ggcttgggcg
    36961 cggctgatga ttttgtcttg tttgctgtgt tggtgtgcgg cgtgtccgtg gtcgtcatcg
    37021 ccgtgtccgt cgtggtgggc gagtgcgggg gcggaaatgc ccatcagtgc ggttgcggcg
    37081 gcggtcaaga gaaggcgttt gaagttcatg ttttgctcct gcgaagtatc tggtggtgtt
    37141 tgaaagacgt aaaggcggga catcaaccgg cggtcaatac cgtctgaacc ataccgtgcg
    37201 cctgagcttc ggcctcggcg gcgcgttcct gcgaggcaaa cggacccatt ttgacgacgt
    37261 attcgtaacg gcgtttttca atggcgaggt tcgggcttga tgtcgaagcg gcgaagtttt
    37321 gggcggcttg gttcagatag gcttgtgctt cgtgttccgt accgaaagat ttcaagtcga
    37381 taaagatgtc tttgttttcg gcaaccggtg cggatttgcc cgggacgatt tgttcgattt
    37441 tgacgtgcgc cgtcccttgg ctgacaaagc ccaatttttg cgcggcggct ttggatacgt
    37501 cgatgatgcg gttgccgtgg aaggggccgc ggtcgttgac gcggacgatg acgcttttgc
    37561 cgtttttggt attggttacg cgcacatagc tggggatggg cagggttttg tgggcggcgg
    37621 taaaggcgtt catatcgtat cgttctccgc cggaagtttt gcgcccgtga aacctgccgc
    37681 cgtaccacga ggcgttgccg gtttgcgtga attcggcgac ttggtttttc ggcgtgtagc
    37741 gttttccggc gactttgtag ctgcggttgg cggaggcgtg cagtttttct gccttgacca
    37801 ctgcgtcggc ggatgccgtc tgaagggagt gtgtgccgaa tgcggcggtg agaaggaaaa
    37861 gggtttttcg ggttaaagtc aaaacgtgtt ccgttcttga gttgaagacg aatgggcatc
    37921 atgcccgccg gatacgttcc gaaccgccgt acagtgcgga cggcggttcg gaatgtgtcc
    37981 ggataggttt tcagacggca tgaacttgcg ttcaaacgcc gcctgcgtaa ccgtgttgcc
    38041 gccacgcttc aaagagaatc acggcaacgg tattggaaag gttcatactc cggctgccgg
    38101 gctgcatcgg caggcggatt ttttgcgcgg cgggcaggct gtcgaggatg tcggcaggca
    38161 gtccgcgcgt ttccggcccg aacagtaaaa cgtcgccttt ttgaaacgcg gtttcatcgg
    38221 ggcgcgccgt gcctttggtg gtcagggcga aaatgcgcct gcctgcgagt gccttgaggc
    38281 agtcgtcgaa gttttcgtac accgtcaggc tggcgaattc gtggtagtcg agcccggcgc
    38341 gtttcatttt ggcggaatcc aatgggaagc cgagcggttt gacaaggtgc aaatccgcgc
    38401 cggtattggc gcacaggcgg atgatgttgc ccgtgttcgg ggggatttcc ggttggtata
    38461 aaacgatggt aaacataaat atcaatcact tataggcgcg taaccttgcc acaaggcgga
    38521 tggggtgtca aaaaatttag ttattttttc attggcgtgc gtgccagcgt ccagcagcag
    38581 atgcggtttg cgcctaattt tttcagcgtc tttgccaatt cgtccagcgt cgcgccggtg
    38641 gtaaagacat cgtcgattaa cagaatatta cagttttccg gtatcggtgt gcggatttca
    38701 aaggcgtttt tgatgtttcg ccgccgttcg ccgcctttga gcgtgctttg cggcgggcgg
    38761 tggtgtcgga aaacggtgtg tcggggcagt atctgccagc cgtagcgttg tgccagcagc
    38821 ccgacgatgc tttcactttg gttgaacccg cgttgcagca gccgctccct gcttagcggt
    38881 acgggcagga cgaaatcgaa acattcgtct gcaagccggt cgggcggatt ctgcatcatc
    38941 aggtctgcca gcggctgcac catgctcaaa tcagccaagt gcttcagcgc gtgtatcata
    39001 ttgctgacgg gcggttcgta atgcagcgag gcccacatcc ggtcgaatgc gggcggtttc
    39061 ctttggcaaa tgccgcacac cgcgccgcct tggatgtgtc tgaaacacag ggggcagctg
    39121 tttgccgcgt cggtgcggta tgccgccaaa tcgttgcggc agccggcgca gatgccgtct
    39181 gaaacgccgg acgaaccgtg gcataatacg caacgcctga tagtgggcgc gtctgcgatg
    39241 cgccgccaac aagagagaaa atccatgcct gatgccgtca aaaaagttta cctgatacac
    39301 ggttgggggg cgaaccgcca catgttcgac gatttgatgc cgcgcctgcc tgcaacgtgg
    39361 ccggtgtccg ccgtcgattt gcccggacac ggcgacgcgc ccttcgcgca acccttcgat
    39421 attgaggcag ccgccgacgc tgttgccgca caaatcgata cgcccgccga cattctcggc
    39481 tggtcgctcg gcggattggt cgcgctgtat ctggcggcgc gccatcccga caaagtccgt
    39541 tcgctctgcc tgacggcgag tttcgcccga ctgacggctg acgaagacta tcccgaaggg
    39601 cttgccgcgc ctgcattggg caaaatggtc ggcgcgttcc gttcggatta tgccaaacat
    39661 atcaaacagt ttctacaact ccagcttctg cacacgcctg atgcggacgg aatcataggc
    39721 agaatcctgc ccgatttggc gcgctgcggc acgccctccg ccttacagga agctttggac
    39781 gcggcggaaa gggcggatgc gcggcatttg ttggacaaga tagatgttcc ggtactgctg
    39841 gtgttcggcg gcaaagacgc gattacgccg ccgcgtatgg gcgagtatct gcaccggcat
    39901 ttgaaaggca gcaggctggt tgtgatggaa aaggcggcac acgcgccgtt tttgagccat
    39961 gcggaagcgt ttgccgcgct gtaccgcgac tttgtcgaag gggtttgaca tgaatccgca
    40021 ggacaaatgc tggcaggttc accgccatct cgccgaatat gccgaccaac gcctgacact
    40081 cgtccgcaac gcgcccaagt atatcctgct tgccggtgcg gatgcggaca tcagccgcag
    40141 cctgctggcg aaacgctatc cgcaggcggt atttgaagaa tacgattccc gtgcggactt
    40201 tttggcggct gccgctgccg cccgcaaagg cggtttttgg caaaggttta cgggtaaggg
    40261 cgtggtgcaa cactgccaat ccccgatcgc gccgctgccc gaagcgtgtg ccgatatgtt
    40321 gtggtcgaat ctcggactgt tggcggcgga acaaatcctt cctgtgctgc acaactgggc
    40381 gtgcgccttg aagacggacg ggctgctgtt ttttacctgc ttcgggcgcg acacttttac
    40441 cgaactgaaa tgccgtctga aagaaaacgg cattgaaagc cgcagcgtga tgtttcccga
    40501 tatgcacgac ttgggcgata tgcttgctga aaacggcttt tacgaccccg ttaccgatac
    40561 ggcgaagctg gtgttggatt acaaaaaggc ggaaacgttt tgggcggata tggacacgct
    40621 gggcatctgg cgggcgatgg cgtggaacga tgaaaacgcc gcgcgttcgt gtgtcgggac
    40681 aatatttgag cgggaaggcg gtttgggcat tacgctggaa acggtgtacg gacacgccgt
    40741 gaaaaaactg atgctgccgc agggggagaa cgtggtgcag ttttttccga agagatgatg
    40801 tgcagatgcc gtctgaagcc gtgtccaagt ttcagacggc atctgtctgt gaaaaccgat
    40861 agaaataaag gaaataccga tgtatagtga attaaatt
//
LOCUS       DAWFIE010000007        39980 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWFIE010000007 DAWFIE010000000
VERSION     DAWFIE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39980
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.007"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(88..1974)
                     /gene="uvrC"
                     /locus_tag="WGQ71_000342"
     CDS             complement(88..1974)
                     /gene="uvrC"
                     /locus_tag="WGQ71_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ0706414.1"
                     /translation="MPSETVFQVIDMNKETRFPEHFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNLI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IINNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPHSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLGDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(2038..4200)
                     /locus_tag="WGQ71_000343"
     CDS             complement(2038..4200)
                     /locus_tag="WGQ71_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ0706415.1"
                     /translation="MQQKIRFQIEGMTCQSCASRIEKVLNKKDFVESAGVNFASEEAQ
                     VTFDDSKTSAADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLAINIPFLIG
                     MAGMMIGRHDWMIPPLWQFALASIVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAHGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTLLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPTVVGIALLTFITTWLVKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYCVPDSGFDEDALYRI
                     AAAVEQNAAHPLARAIVSAAQARGLGIPAAQNAQTVVGAGIAAEVESVGLVKAGKAEF
                     AELTLPKFSDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPIIAGAAMAASSVSVLGNALRLKRVKID"
     gene            4339..5289
                     /gene="trxB"
                     /locus_tag="WGQ71_000344"
     CDS             4339..5289
                     /gene="trxB"
                     /locus_tag="WGQ71_000344"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ0706416.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5445..5813
                     /gene="rpsF"
                     /locus_tag="WGQ71_000345"
     CDS             5445..5813
                     /gene="rpsF"
                     /locus_tag="WGQ71_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ0706417.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            5814..6116
                     /gene="priB"
                     /locus_tag="WGQ71_000346"
     CDS             5814..6116
                     /gene="priB"
                     /locus_tag="WGQ71_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ0706418.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGISVLDIILKHESWQKENGQ
                     QCFVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIKEYKG"
     gene            6123..6353
                     /locus_tag="WGQ71_000347"
     CDS             6123..6353
                     /locus_tag="WGQ71_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ0706419.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            6370..6822
                     /locus_tag="WGQ71_000348"
     CDS             6370..6822
                     /locus_tag="WGQ71_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ0706420.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARARQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            6836..6943
                     /locus_tag="WGQ71_000349"
     CDS             6836..6943
                     /locus_tag="WGQ71_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ0706421.1"
                     /translation="MPSETLPIIGMWFQTVFVLFSVYSAKIGMVVFIVD"
     gene            complement(7780..8586)
                     /locus_tag="WGQ71_000350"
     CDS             complement(7780..8586)
                     /locus_tag="WGQ71_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0706422.1"
                     /translation="MWHWDIILILLAVGSAAGFVAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFAMMPGMIFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGPQTASRPLPKLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(8587..9333)
                     /gene="pssA"
                     /locus_tag="WGQ71_000351"
     CDS             complement(8587..9333)
                     /gene="pssA"
                     /locus_tag="WGQ71_000351"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ0706423.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            9917..10399
                     /locus_tag="WGQ71_000352"
     CDS             9917..10399
                     /locus_tag="WGQ71_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ0706424.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(10451..11662)
                     /locus_tag="WGQ71_000353"
     CDS             complement(10451..11662)
                     /locus_tag="WGQ71_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ0706425.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            11885..14323
                     /locus_tag="WGQ71_000354"
     CDS             11885..14323
                     /locus_tag="WGQ71_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ0706426.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDEPEPLEKLPLIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPAD
                     LNGSRKILAHKDHL"
     gene            14532..15842
                     /locus_tag="WGQ71_000355"
     CDS             14532..15842
                     /locus_tag="WGQ71_000355"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ0706427.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADPSRLQAPTSLAVESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            15938..16552
                     /gene="clpP"
                     /locus_tag="WGQ71_000356"
     CDS             15938..16552
                     /gene="clpP"
                     /locus_tag="WGQ71_000356"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ0706428.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(16608..17873)
                     /gene="ispG"
                     /locus_tag="WGQ71_000357"
                     /gene_synonym="gcpE"
     CDS             complement(16608..17873)
                     /gene="ispG"
                     /locus_tag="WGQ71_000357"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ0706429.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(17889..18650)
                     /gene="pilW"
                     /locus_tag="WGQ71_000358"
     CDS             complement(17889..18650)
                     /gene="pilW"
                     /locus_tag="WGQ71_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ0706430.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(18653..19747)
                     /gene="rlmN"
                     /locus_tag="WGQ71_000359"
     CDS             complement(18653..19747)
                     /gene="rlmN"
                     /locus_tag="WGQ71_000359"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ0706431.1"
                     /translation="MKTNLLNYDLQGLTRHFADTGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQASIEIPKLMMSQESSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDRAQHAR
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(19890..20315)
                     /gene="ndk"
                     /locus_tag="WGQ71_000360"
     CDS             complement(19890..20315)
                     /gene="ndk"
                     /locus_tag="WGQ71_000360"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ0706432.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(20374..21525)
                     /locus_tag="WGQ71_000361"
     CDS             complement(20374..21525)
                     /locus_tag="WGQ71_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ0706433.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            complement(22061..22888)
                     /gene="fghA"
                     /locus_tag="WGQ71_000362"
     CDS             complement(22061..22888)
                     /gene="fghA"
                     /locus_tag="WGQ71_000362"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ0706434.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNRE
                     HYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(22897..24006)
                     /locus_tag="WGQ71_000363"
     CDS             complement(22897..24006)
                     /locus_tag="WGQ71_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ0706435.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPAKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFELMHEGKSIRAVIHY"
     gene            24154..24561
                     /locus_tag="WGQ71_000364"
     CDS             24154..24561
                     /locus_tag="WGQ71_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ0706436.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            complement(24912..25226)
                     /locus_tag="WGQ71_000365"
     CDS             complement(24912..25226)
                     /locus_tag="WGQ71_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ0706437.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(25237..26922)
                     /gene="rpsA"
                     /locus_tag="WGQ71_000366"
     CDS             complement(25237..26922)
                     /gene="rpsA"
                     /locus_tag="WGQ71_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ0706438.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(27077..27733)
                     /locus_tag="WGQ71_000367"
     CDS             complement(27077..27733)
                     /locus_tag="WGQ71_000367"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ0706439.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            complement(27977..29022)
                     /locus_tag="WGQ71_000368"
                     /pseudo
     CDS             complement(27977..29022)
                     /locus_tag="WGQ71_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(29159..29407)
                     /locus_tag="WGQ71_000369"
     CDS             complement(29159..29407)
                     /locus_tag="WGQ71_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ0706440.1"
                     /translation="MKRHPTLQPLSRQHHLGLVIANKAKSVTDDDKLTQHQAFILTVA
                     GATVGFGATWRFPYLVGENGGARMCFYSVSRCWLSASR"
     gene            complement(29501..31818)
                     /locus_tag="WGQ71_000370"
                     /pseudo
     CDS             complement(29501..31818)
                     /locus_tag="WGQ71_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237991.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(31865..33121)
                     /locus_tag="WGQ71_000371"
     CDS             complement(31865..33121)
                     /locus_tag="WGQ71_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0706441.1"
                     /translation="MIISQQKNRHLLTMVALLYVAHALPLYFFNVALPAILRHQGVDI
                     RVIGMLSLLYLPWAFKFLWASYVDNYYCAKIGKRKTWLFFTQIIVVVGLFGLGFLQFS
                     ESEIVLFLSISFLVSLASATQDIAIDGYTVEQFDKSSFGLASAMQSFGVAFGSMVGGA
                     GTLWLYQRFDWQMATISLATTVVLISCSLWFLKDQDNPKHATNLSRASLIRLIKRPQT
                     KWLLALIVVFRFVEAPMMAMLNPMLVDFGFSLSQIGLLFSVFGAIVGIGSALLAGVLI
                     KYIDIVRCLILFGWLRTAVYALLCLGLLYPLLGDFLPAVGLLKTGFMSLMAISVLLIL
                     SVRYLTMTALYALFMNAANPDQAGTDFTLFVCVELLVYFIGGAMSGFLVHQFGYDKLF
                     LWLAIGSGVSMLILPTLIKKFSVTDC"
     gene            complement(33121..33519)
                     /locus_tag="WGQ71_000372"
     CDS             complement(33121..33519)
                     /locus_tag="WGQ71_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706442.1"
                     /translation="MPIFLNAIQNPNSSLPVFLLDFSGEISQDAFDDWLIAMSDLLAS
                     EQSFALIYHSRTGLTLPNNYRQIEAVWYKTHKESFFKYCKGLVRIVDDETQRQALDSP
                     AFHKAWRVPYAVVLSLTEAKAWINTKIGAV"
     gene            complement(33524..34126)
                     /locus_tag="WGQ71_000373"
     CDS             complement(33524..34126)
                     /locus_tag="WGQ71_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ0706443.1"
                     /translation="MPTLSQGLPNNYIYRENGVEFGLVQIRYPEHIELLHRWMNMPHV
                     IPQWQLNKSLSELSVYFEKMLADDHQRLYLVSINGEWVGYAEVYEGARDRLSGYYTAD
                     KNDLGWHLLLAEAQAVGKGYLRAIMRLLSFFIFEHSPAKKIVGEPDASVKPYEVVAQE
                     LNYMPQYQIVMPEKTAMLYFCDKEGFYCKFAHYLNASIKE"
     gene            complement(34623..35315)
                     /locus_tag="WGQ71_000374"
     CDS             complement(34623..35315)
                     /locus_tag="WGQ71_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEZ0706444.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            complement(35383..37359)
                     /locus_tag="WGQ71_000375"
     CDS             complement(35383..37359)
                     /locus_tag="WGQ71_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0706445.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDIYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIVRLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIVPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRVRLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            37538..38203
                     /locus_tag="WGQ71_000376"
     CDS             37538..38203
                     /locus_tag="WGQ71_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0706446.1"
                     /translation="MDSWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     IAD"
     gene            38255..39082
                     /gene="mutM"
                     /locus_tag="WGQ71_000377"
     CDS             38255..39082
                     /gene="mutM"
                     /locus_tag="WGQ71_000377"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ0706447.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCTDYLYVRLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFMDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            39170..39937
                     /locus_tag="WGQ71_000378"
     CDS             39170..39937
                     /locus_tag="WGQ71_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0706448.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGALTALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
BASE COUNT         9802 a         9511 c        10470 g        10197 t
ORIGIN      
        1 gtactggttt aaatttaatc cactatatat cgatataccg gcctgctgta tcatattttt
       61 acagacggtt tttgttttgg cggcatacta atgcagatga ttataaatgg tttccgccaa
      121 tgccttgctg atgccttcca ctttttccaa gtcctcgcgg ctggcggcaa tcacgccgcg
      181 cagaccgccg aagcgggtaa gcagggcttg gcggcgtttg ctgcctacgc cgggaatgtc
      241 gccgagggat gaggtaacgc gcgctttgtc gcgttttttg cggtggcctg tgatggcaaa
      301 gcggtgcgat tcatcgcgta cggtttgcaa tagatgcaag gccgggctgt gtgggggcag
      361 gcggaagact tcgccggtaa aaggcagtat gagttcttcc ataccggctt tgcgctccgg
      421 gcctttggcg ataccgacca aaggaatgtg cagcccgagt tcttcccata ccgatacggc
      481 tacgccgatt tgccctttgc cgccgtcaat caatacgaca tcgggccatt tgacggtctc
      541 gccgttggct tcggcctcct gcattttgcc gtaacggcgc gtcaacactt cgcgcatggc
      601 ggcgtagtcg tcgccgggtt tggcggtcgt gatgttgtag cggcggtatt gcgagggctg
      661 gatgttttgc tcatcgtaca caacgcagga cgcaatagtg gcctcgcctt gggtgtggct
      721 gatgtcgaaa cattcgaggc ggttgaggcc gtctgaatcc ataccgagga ttttggcgag
      781 ttcgtcgatg cggtgttgct ggctgctttg ttgcaggcgg cgttgtgcaa tcgccatttg
      841 tgcgttttgc tccgccattt tcaaccagac tttgcgttcg cctatggttt tggttacgaa
      901 ctgcatttgt ttgccgtgtt cgccttccaa agcctctttc agcgcatcgg ggacggggaa
      961 gttgttgatg ataatgtcgg gtttgctttt gcccagatag tgttgggcga cgaaggcttc
     1021 ggcatagtct tgcccgtttg gctcgggatc gtttttggtg tcggggaaga aacttttgtc
     1081 tccgacgtgc cgcccaccgc gtatgctgac ccagtgtacg cagacgatgc cgtctgaaac
     1141 cgccagcgcg agcaaatcga tatcgttggg gttgttcggg tttttgctgt cgatgaactg
     1201 gttgctctgc ataatgccga gtgcttggat ttggtcgcgg tagcgggcgg cttcttcaaa
     1261 ttgcagattc gcggcggcag tttgcatttt gtgttgcagg gtgcgggtca gttcgtcggt
     1321 tttgccgttg agaaaagtgg cggcttgacg cacgctgtcg cggtagtctt cttcgctgat
     1381 gtgtccgacg cagggcgcgg tgcagcgttt gatttgatac agcaggcaag ggcggtcacg
     1441 atgctcgaat acgctgtctt cgcaggtacg cagcataaag actttttgca acacttgaat
     1501 gctgtcgcgc acggcgttgc tgttgggata cgggccgaag tattggtttg gctttttcag
     1561 cgtgccgcgg taatacgcca tctgcggata ttgatggccg ctgagcatca aataaggata
     1621 gcttttgtcg tcgcggaaaa ggatattgta tttcggcgac aaggctttga tgaggttgtt
     1681 ttcgagaatc agggcttcgg cttcggaacg cgtgatggtg gtttcgatat gatgaacctg
     1741 ttttaccatc aatgcgatgc gcggcgaatg gtcgtttttt tggaaatagc cggacacgcg
     1801 ccgcttgagg ttgaccgctt tgccgacgta taagactttg ccgccttcgt caaaaaaacg
     1861 gtacacgccc ggcagcttgg gcaggttttt gaggaaaagt gggatgtcga agtgttccgg
     1921 aaaacgggtt tctttattca tatcaattac ttgaaaaacg gtttcagacg gcatattacc
     1981 atatcgctgg agacagcctt atccgttcgg acaaggctgc agggcggtta catgctgcta
     2041 atcgattttc acccgtttca ggcgcaaggc attgcccaac acggaaaccg agcttgccgc
     2101 catggccgcg cctgcgatga tgggatttaa aaagccaagc gcggcgagcg gaatgcccaa
     2161 gatattgtag aagaaagcga aaaacaggtt ttgcttgatg tttttcaaag tggcttgcga
     2221 caccagcagg gcatcggcga gctgattaac cgaatgctgc atcagcgtgg cggacgcggt
     2281 gtgttcggca acgtccgccc cgcctttcat cgcgaagcta acattggcgg cggcgagcgc
     2341 gggcgcgtcg ttgatgccgt cgccgaccat cgccacggtt ttgccggcgg ctttgagttt
     2401 ttgcacttcg gcggctttgt cgcgcggact catattaccg aaggcgtgtg cgatgcccag
     2461 ttgtttggcg acgtattcga ccgtgccttg gttgtcgccg ctcataatat agacatcgat
     2521 attgtgtttt ttcagacggc ctatggcttc ggcggtatcg gctttcaacg cgtcggcaag
     2581 tgcgaatgcg ccgatgggtt tgttatcgac cgagactgca acaatgcttg caatgtccca
     2641 aacgccgtct gaaaacttcg gcaaggtcag ttcggcaaat tcggctttgc ccgctttcac
     2701 caaccccacg ctttccactt cggcggcaat gcctgcgccg acaacggttt gcgcgttttg
     2761 tgcggcggga atccccaagc cgcgcgcttg ggcggcggaa acgatggcgc gggcaagcgg
     2821 atgagcggcg ttttgttcga cggcggcggc gatgcggtac aaagcgtctt cgtcaaagcc
     2881 gctgtcggga acgcaataga cggcggcaac ctgcggcctg ccttcggtca gcgtgccggt
     2941 tttgtccaac acgacggcat cgacgtgggc ggcttcttcc attgccgccg cgtctttaaa
     3001 ccaaatgccg tgtttcactg ctttacccat gccgaccata atcgcggcag gggtcgccag
     3061 accgagcgca catgggcagg caatcaccaa aacggcaacg gcatgcatca gcgcaaccgt
     3121 ccaatcgcct ttcaccagcc aagtggtgat aaaagtcaaa agcgcaatgc ccacaacagt
     3181 tggcacaaac accgccgccg ctttatcggc tacgcgcgca atcggtgctt tactgccttg
     3241 cgcttcggat agcgcgttca tcatgtcgcc aagcagggtt tggctgccga gctgcgtggc
     3301 gcggtacacc acgctgcctt cggtcatcag cgcgcccgcc aacactttgc ctcctgcctt
     3361 tttctcttcg ggattggatt cgccggtgag gtggctctcg tccgcccagc cgctgccgct
     3421 ttcaatgatg ccgtcggcgg caatgcgttc accgtggttg gcgcggatta gatcgccgat
     3481 ttgcacttgg tcgatgggaa gctgtttcca ttcgccgttg cgttgtacat tgacttgggt
     3541 cggcgtgagt ttgagcagca aacccaagct gttcaggctg gattttttgg tgcggtgttc
     3601 caaaaattta cccagtgaca caaaaccaat caccattacg cccacttcaa aatacacatg
     3661 cgccataccg tgcgccgcgt gcgggctgaa aaacagcata tagacggaat acaggtaaat
     3721 cgacaccgta ccgatggtaa cgagcacgtc catattcgcc agtccgccct taatgctcgc
     3781 ccacgcgctt ttgtaaaacg ggatggccag ccaaagctgc acaatgcttg ccaatgcaaa
     3841 ctgccacaac ggcggaatca tccaatcgtg ccgcccaatc atcatgcctg ccatgccgat
     3901 taagaacggg atgttgatgg ccagcaaaag ccacagcctc cagccgatat ggtgttccgc
     3961 ttcaggttgc ggcaatgtat cttccgtctt ttccttcgca ccgtaaccgg ttttctcaat
     4021 gattttggca atgtcggcag cggaagtttt gctgtcgtca aacgtaacct gcgcctcctc
     4081 gctggcaaag ttcacccctg ccgattcgac aaaatctttt ttgttcaaca ctttttcaat
     4141 acgcgaagca caggactgac aagtcatgcc ttcgatttgg aaacggattt tttgttgcat
     4201 aagtttgatt cctgctttga tgttcagacg gcagtcaagg ttgcgggatg ccgtctgaac
     4261 gcccgtatgg tcaaaatttc tcaaacgcgc cgataattcg agtagaatcc gaaaacttga
     4321 cacagcaaag gaagaacaat gtcccaacac cgcaaactga ttattttggg ttccggtccc
     4381 gccggataca ccgccgccgt ctatgccgcg cgcgccaatt taaaccccgt cattattaca
     4441 ggtatcgcgc aaggcgggca actgatgacc accaccgaag tggacaactg gcctgccgat
     4501 gccgacggcg tgcaagggcc ggaattgatg gcgcggtttc tcgcccacgc cgaacgtttc
     4561 ggaacggaaa tcatttttga ccaaatcaac gccgtcgatc tgcaaaaacg cccgtttaca
     4621 ctcaaaggcg atatgggcga gtacacttgc gatgccctga ttgtcgcaac aggcgcgtcc
     4681 gccaaatacc tcggtttgcc gagtgaggaa gcgtttgccg gaaaaggcgt ttccgcttgt
     4741 gccacctgcg acggtttctt ctacaaaaac caagatgttg ccgtagtcgg cggcggcaat
     4801 acggcagttg aagaggcact ctaccttgcc aatatcgcca aaaccgttac cctgatccac
     4861 cgccgcagcg agttccgtgc cgaaaaaatc atgatcgaca aactgatgaa acgcgtggaa
     4921 gagggcaaaa tcatcctcaa gctggaaagc aacctgcaag aagtactggg cgacgatcgg
     4981 ggcgtaaacg gcgcattatt aaaaaacaac gacggttctg agcaacaaat tgccgtcagc
     5041 ggcattttta tcgccatcgg gcacaagccg aataccgata ttttcaaagg tcagttggaa
     5101 atggacgaag ccggttacct gaaaaccaaa ggcggcacgg cggacaatgt cggcgcaacc
     5161 aatatcgaag gcgtatgggc ggcgggcgac gtaaaagacc atacctaccg tcaggcgatt
     5221 accagcgcgg cttccggctg tcaggcggct ttggacgcgg aacgctggct gggcagccaa
     5281 aacatttgaa tgccgtctga aaccgaaatt ttcggcggaa tgcaagtttt tcgcaccaaa
     5341 tccttgattc cgtcggcaaa ccccaatata atgcctgctc tttgaacctt gctttctgct
     5401 ttcgcatggc agaaggctgt ttttaccatc cataaggaga taacatgcgt cattacgaga
     5461 tcgtgtttat cgttcatcct gatcaaagcg agcaagtgcc tgctatggtt gaacgttaca
     5521 aaaccatgat tgccgaagca aacggtaaga ttcaccgtct ggaagattgg ggtcgccgcc
     5581 aactggctta cccgattaac aaaatccata aagcccatta cgttttgatg aacatcgaaa
     5641 ccactcccga agtggtcgaa gagttggaaa ccgcgttccg tttcaatgat gcggtattgc
     5701 gtcatctgac catcaaaacc aaacacgccg ttaccgaagc atcccctatg ttgggtggcg
     5761 aaaaggctaa gaacctgctg agcggtgcgt ctgaagaagc ggttgcccaa taattgggat
     5821 tcaataatct tgtttcgctt gccgcgttaa ttgaaaaggt tttccctatt cgatatacgc
     5881 ctgccggaat ctctgtctta gatattatct taaagcatga atcatggcag aaagaaaatg
     5941 gacagcaatg ttttgtccaa ttggaaattc cggcacggat tttaggcagg caggcggaag
     6001 agtggcagta tcggcaaggt gtatatgttc acgtcgaagg ttttttggct caaaaaagca
     6061 gacgttccct gatgccgatg ctcagaatac aaaatattaa agaatataaa ggttaaacga
     6121 caatggctcg tcaatcattc aaacgtagaa aattctgccg tttcacggct gaaaaaatcc
     6181 aagaagtcga ttacaaacaa gttgatttgc tgaaagactt tatctctgaa aacggtaaaa
     6241 tcattcctgc ccgcatcaca ggaacgaagg cattttacca acgccaattg gctgttgccg
     6301 taaaacgcgc gcgcttcttg gctctgctgc cttacaccga ccaacacaaa taattttgga
     6361 gattaaatca tgcaaattat tctgttagaa aaaatcggcg gtctgggcaa cttgggcgac
     6421 atcgtaaccg ttaaaaacgg ttatgcccgc aacttcttga ttcccgcagg taaggcaaaa
     6481 cgtgctactg aagcgaacat gaaagagttt gaagcacgcc gtgccgaatt ggaagccaaa
     6541 caggccgaaa ttttggcaga tgcccgagcc cgtcaggaaa aattggacgg tcaaaccgtt
     6601 accgttgcac aaaaagccgg tgtggacggc cgcctgttcg gttccgttac caatgccgac
     6661 attgctgctg caatcgttgc tgccggcatc gaagccgtga aagcaaatgt acgtctgccg
     6721 aacggtcctc tgaaagccgt tggcgagtac gaagtggaag tggctttgca cactgatgcc
     6781 gttgctaaaa ttaccgttgc cgtcgttgcc gcaaccgagt aattgaaatt atcagatgcc
     6841 gtctgaaacc ctgccgatta tcggaatgtg gtttcagacg gtatttgttt tgttttccgt
     6901 atattctgca aaaatcggga tggttgtttt tatagtggat taaatttaaa ccagtacagc
     6961 gttgcctcgc cttgccgtgc tatctgtact gtctgcggct tcgtcgcctt gtcctgattt
     7021 ttgttaatcc actatatttg cctgtggtct ttgcaggaat agtttgttaa cgaaatttga
     7081 tgcataaaat gcgccaaaaa atattggctg tgttttaata tagtgttgat aagcgtacct
     7141 ggcttcaata tactcaatag gataggtctc ttcctaaccg aaaaaataat tgtcttttcc
     7201 cttgcaaata attgaaatca acagattact aaaacacagc caaaatattc aattgacaaa
     7261 aacttttctg attttgagaa atctgtgaaa atcaggggat tatttatttt gaaaatggta
     7321 tcgggcataa aatttggctg tgtttcaata tggtgctgat aacatacttt gtttcaaaat
     7381 gttcaatagg ataacttcct gattgaccga aaaataatgg ccttttccct tgcaagcagt
     7441 tgaaatcaac agattgtcaa aatacatcct aaaaatttaa taacttatat gattgcaaag
     7501 gttgcggctt gtatgccggt cattatggcg gaaataccgt ttgggaggca gccgcatcgc
     7561 gtggaatcgg cacggcaggc gatgcgccga tgccttcggg cgtgaaggcg gggcaatcgg
     7621 cagtttggaa aaaggcaatg gaatctgtgg gcagaagaag ttaagggtaa aagtcgggcg
     7681 cacggccagg ttgtctgtcc ttttgtcgga acaggagact gggcagcttt gtcctgatta
     7741 attgcttgtg caaacggtaa aaagggtttt tcgtgtgttt taaagcaggt tgtacagcat
     7801 ttttccggca atcaaaagca gcataatgcc gaaggatttt ttgagtttgg cagaagaaag
     7861 tttgtgggcg gttttgacac cgagcggggc aaaggcaatg gttgccgcgc tgaggacggc
     7921 gacggcgggc aggtaaagga agcccagcga cccttcgggc aatcctgcaa tattcaggcc
     7981 gttgagcaga tacgatattg cgccggagag tgcaatcggc caggcaaggc cggatgatgt
     8041 gccgatggct ttatgggcgg ggaagccgca gtggattaag aaggggacgg aaagtgaacc
     8101 gccgcctatg ccgacccagc tcgacattgc gccgaacagt gtggaaaccg cagtcagtcc
     8161 gggcagcttg ggcagcgggc gggatgccgt ctgagggcca gtatgcagtg ttttgaatgc
     8221 gacggcagtt aaaaacagga tgaagaaaat ttgaagcccg aatgcgggga tatattttgc
     8281 ggagagtgcg cccgcgaata cgccgaatat catacccggc atcatcgcaa atacggtttt
     8341 ccagtcgacc gcctgttttt tgtgctgccc caacatactg gaaaaggcgg tgaagaccat
     8401 gacggcgaag gatgtgccga cggcgaggtg ttgcgcgtaa ggatgttgtg ccaaaccctg
     8461 caaatcaagc acccataaaa cgacagggac aatcagcgtg ccgccgccta cgccgaacag
     8521 gccggcgaca aaacctgccg cactgcctac ggcaagcagg attaagataa tgtcccaatg
     8581 ccacatttaa tccgcctttc tgtatttttt ccagaatcgg cgtgccgcca taatgtagcc
     8641 ggacaggctg tatccgagaa agaacaggaa gaggacgagc gacggttccc aagtgaccag
     8701 aagcagcagt aagacggcaa gcagcattcc gacaaagggg acttgtctgc ggatgttgat
     8761 ttctttaaaa ctccaaaaag ggatttggac aatcatcgac agaccggcaa acagtgtgat
     8821 gcccaatgcc caccagtgga cggcggggaa tttttcgacg ctgtggttga cccaaatcag
     8881 cccgacaatc agcgcggcag cagtcggact gggcacgccg ataaaccagc gtttgtccac
     8941 cttgccgatg agcgtattga acagcgcaag gcgcagggcg gcgcaggcgc agtagatgaa
     9001 ggcgacggaa taaccgattt tgccgaactg ccaaagttgc catttgtagg caataagcgc
     9061 gggggcaacg ccgaagctga ccatgtcggc aaggctgtcg agctgttccc cgaacgcgct
     9121 ttggctgttg gtcagccgcg ccacgcgccc gtccataccg tccaaaagca tggaaatgaa
     9181 tacggcaata gcggcggttt cataacgtcc gtgcatggat tgggtgattg cgtaaaacgc
     9241 ggaaaacagc gcggcgatag taaaggagtt gggcagcagg tagatactgt tttgacgcag
     9301 ggagcgttgc ggggtgttgg tcggggtttc catagctgaa gtgtctcacg gacgtggaga
     9361 atcgataggg gattataacc caaaccatta ttcgaacaat acgcccttta ggatgaagcc
     9421 ctcggaggcg gtttgttttt ctgatggcgg atgctttgcg gggcggtttg actctgcctg
     9481 ccaccgggtg tgttgtttcg gatttttgcc ggatggatat tgttgccggg catttctggg
     9541 cttgccggaa ggtatcgggg ggagggcatc ggaatatgcg gtgcgccgtt caaaccgggg
     9601 cggcgtttcc ctattttcag ggggttgcag gtttcaacaa attgaaagat attttttgca
     9661 tttccgaccg tatgctgatt ttgaagcggg ataaattata ggacaaggtt tttcgcccgc
     9721 cgcaaaggtt ggccgcaatg ccgtctgaaa acaacggctt gccgctaagg cggcggtatg
     9781 cggatttgcg ctgccgaaat cggtttgcca tacccggcgg tgcaatctgc ggggagggat
     9841 aagccgataa ggcgggactt atcctgtttt ttgtttataa tgcaacagaa tttggacgaa
     9901 ccgtcgggaa tttatcatga tgaaaaagat actggccgta tcggcactat gcctgatgac
     9961 tgcggcggca cgggctgccg atacttacgg ctatctcgcc gtttggcaga atccgcagaa
    10021 tgcggacgat gttttgcagg ttaaaaccac aaaagaagat tcgacgaaaa gcgaagcgtt
    10081 tgccgagttg gaagccttct gcaaaggtca ggacacgctt gcgggcattg ccgaagacga
    10141 gccgaccgga tgccggtcgg tcgtgtcgct gaacaatacc tgtgtcgcgc tggcataccc
    10201 gaaagccttg ggcgcgatgc gcgttgaaaa cgccgtcgtg attacttctc cgcgttttac
    10261 gagcgttcat caggtcgcac tcaaccagtg catcaaaaaa tacggcgtac agggacaatg
    10321 cggcttggaa acagtgtatt gcacatcttc ttcttattac ggcggaactg tgcgctcttt
    10381 gattcaaaat ctcaaataaa acggaaaatg ccgtctgaaa gatgttcaga cggcatttct
    10441 atatcgacgg tcaggattct ttcggatcgg gcagcaggct gttcaacata atggcaagta
    10501 cggcgcacaa gcccacgccg gcaaagctga agctgcccaa tttgagcgtc atgccgccga
    10561 tgcccgtggt cagtaccgag ctgacgatga ccaggttttt cggcagcatc aaatcgactt
    10621 tggcatcaat cagcgttttc acgcccaaag aagcaatcgt gccgaacagc agcagcataa
    10681 tgccgcccat tactggcatc ggaatggaag ccaaaaacgc attgaatttg ccgaaaaacg
    10741 ccatgcagac ggcaaaaacc gccgcccaag tcatgatgac ggggttgctg tttttggtaa
    10801 tcatcaccgc acccgttact tcgccgtagg tcgtaaccgg cgggccgccg atcagacccg
    10861 caacgcatac gcccaaaccg tcgcctgcaa gggttttgtc caagcccggg tctttcgtat
    10921 agtctttccc cgtcacattg ccgattgcca tgatgccgcc gatgtgttcg atggcggggg
    10981 cgacggcaac gggcagcata aacagtgcag cctgccagtt gatctgaggc gtttcaaaat
    11041 ggggaacggc gaaccagggc gcgtgtgcaa tgcttgccgt gtccaccagt cccatcagca
    11101 gtgccaaaac ataacccgaa gcgacaccga tcaagatggg aatcagcttc atcatcctgc
    11161 tgccgaaaac cgatacgatg gcggtaacgg caaaggtaaa gccggaaaga atcagcgaat
    11221 cggtatagtc gatgacctgt ttgccgtccg cctgacccat tgccatgctg cttgccgcca
    11281 cggcaacaga cagaccgatg accatgatga cggggccgat gacgaccgga ggcagcagtt
    11341 tgtgtaccgc tgccagtccg cgccaacgga tcagcgcggc aaacacaaaa tacataaagc
    11401 cggcggcaaa cagtccgaac atggtggaag gcagccccca ttcgccgacg gagtagataa
    11461 tcggtgcgat aaaggcaaac gaagaaccaa gaaaaatcgg cactttgcgt ttggttgtga
    11521 tttggaacag cagcgttccc aagcctgcgc ccaaaagcgc aagagccgga ttcagaccgg
    11581 tcagcagggg aaccagcacc attgcgccga atgccacaaa taaaatctgc gcgccggaaa
    11641 cggcaagttt cagttggttc ataccatctt ctttcaatcg gttcaaacgg cacggattat
    11701 actggtttgc cgagggtctg cgtaaaaata catacgtcgg gcaacagata tgccgtctga
    11761 aaggcaatgt ctgtctgttg caggatgaaa gcaggagaaa tggagtataa tccgacactt
    11821 tccatattca atccgcaagc cgtccggctt ccttatttca gacggcattt tccggtaata
    11881 cggcatgaca gaaaaatccc ataaaaaaac agcaaaaggc agggcgggaa gcccgtcccc
    11941 aacgtccgca cgcaacaaga aagccgacaa cggcgcacgg ggcaataaag tttccgagcg
    12001 gctcaaggcg gtcaaagagt tgcagaaaac cgaaaccaaa aaggcgcgtc ccgaacatgt
    12061 cgtcaacctt atcggcgacg cactgtggct gatgggtttg gcggcaaccc tgtatttggc
    12121 gatttccctg atcagtttcg atatgggcga tccgtcttgg tcgcacagtt cgccggttgt
    12181 ggaagatgtc gccaattggg gcggactgtt cggcgcgtat gttgccgatg tcggctatta
    12241 tcttttcggc tggtcgttct ggtggtggat agcggctgcc tgcgtcgtgc tgtataaaaa
    12301 tttccgcctg cacgcaaaac agacggaaaa cgaggcatac aaccacaaaa tcgctgccgc
    12361 cgcgctgttt gtcctgacgg tcttcagccc cgtcttggag tattttgtgc tgggcggaaa
    12421 atatgccgac tccctgcctg tcggagcagg cggtatggtc ggcatacgcg tcggcgcagt
    12481 gtttgcgtgg ctgctgggga aatcgggcag cctgctgatt atcttggttg ttctgctgtt
    12541 gtcgttgtcc ctactggtgc agatttcatg gctggaattt ttgaacggtg caggcagggc
    12601 ggttcaaaac cgcctgagtg ccttatccgg caaggtcatg gctttaggaa aacgccggcc
    12661 gaataccaaa acagacggtg tcgataccca aaatacacgg cgcatggtaa aagaagccaa
    12721 gaatattacg gccaaacccg ttgccttgcc cgaaggcagc agcagcaacc gcaaatccgt
    12781 cgcggtttcc gtcgcgccgc cgcccaaaat tcaggtttct ctgtttgaag atgacgaacc
    12841 tcggcaggcg ggcgaatacc acaagcctac attgaaccta ttgcggattc ccgacagcga
    12901 gcccgtcagc atcaatcccg ccgaattgga gcgcactgcc gaactaatcg aatccaagct
    12961 ggcagaattc ggcatcggcg tacaagtcgt atccgccaca tccggccccg tcatcacgcg
    13021 ctacgaaatc gaacccgcgc aaggtgtcaa aggcagccaa attgttgcct tgtcgaaaga
    13081 tttggcacgc tctatgtcgc tgcaatccgt gcgtatcgtc gaaaccatcg ccgggaaaaa
    13141 cacgatgggc atcgagttgc ccaacgacaa acgccaagac gtgatgttga gcgaaatctt
    13201 gtcctcgccc gtgtttgccg aagccaaatc caagctgacc gtcgcgctgg gcaaagacat
    13261 tgccggtacc cccgttgtcg gcgacttggc gaaaatgccg caccttttgg tcgccggtat
    13321 gactggttcg ggcaagtccg tcggcgtgaa cggcatgatt atgtctatgc ttttcaaagc
    13381 tacgcccgac gaagtccgct tcattatgat agacccgaaa atgctcgagt tgagcattta
    13441 cgacggcatt ccgcacctgc tctgtcccgt cgtgaccgat atgcgcgaag cagggcaggc
    13501 gttgaactgg tgcgtcgccg aaatggaaaa acgctaccgc ctgctttccc acgccggtgt
    13561 acgcaatctg gaaggcttca accaaaaagt cgaagccgca aaagcggcag gcaagccttt
    13621 gcttaatccg ttcagcctga accccgacga gcccgagccg ctggaaaaac tgccgttgat
    13681 tgtggtcgtt atcgacgaac ttgccgacct gatgatgacg gaacgcaaag ccgtcgagca
    13741 gcaaatcgcc cgtctcgccc aaaaagcgcg cgccgccggt atccatatga ttgtcgccac
    13801 ccaacgtccc agtgtcgatg tcgttaccgg cctgattaaa gccaacattc cgacgcgtat
    13861 ggcgtttacc gtgcaaagca aaatcgacag ccgtaccatc ctcgaccaaa tgggcgcgga
    13921 cgaactgctc aaatatggcg attcgctgtt ccttcagccc ggcagtgccg aaccgacccg
    13981 cctgcaaggc gcgtttgttt cagacgacga agtacatcaa gtcgtcaact acgtcaaatc
    14041 gcaagcccca gccgactata ttgaaggtct gctcagcggc gaggccgcgc tggaaactgc
    14101 caatatcgtt aatccgaatg cagacagcga cgaattgttc gatcaggcag tcgcctatgt
    14161 tttggaaagc aaaaaaacct ccatttcgtc tttgcagcgg cagttgcgta tcggctataa
    14221 ccgcgcggca aacctgatgg aggcactgga aaatgcgggt gtcgtttctc ccgccgacct
    14281 caacggcagc cgtaaaattt tggcgcacaa ggaccatttg tagcccgtat tgcaaaatgc
    14341 cgtctgaacg gcggaattcg cgtttcagac ggcatattta tgtttcaggc gaaacattgt
    14401 gatatacttg ccagctaaaa tttccccttt gcggcaatgc ggttcaaata tcgtaccgtt
    14461 gcgctgtttg cttccccatg tagggaagaa agtttatcat tttatcaaca caacaaattt
    14521 aagggcttat gatgagcgta actgttgaaa ctttagaaaa tctggaacgc aaagtagtgt
    14581 tgtccctgcc ttggtccgaa atcaacgcag aaaccgataa aaaactgaaa caaacccaac
    14641 gccgtgcaaa aatcgacggt ttccgtccgg gtaaagcacc tttaaaaatg attgcccaaa
    14701 tgtacggtgc gagcgcgcaa aacgacgtga tcaacgagct ggtgcaacgc cgcttccacg
    14761 atgttgccgt tgcccaagag ttgaaagtgg caggcttccc ccgttttgaa ggcgttgaag
    14821 aacaagacga taaagaatct ttcaaagttg ccgccatttt tgaagtgttc cccgaagtcg
    14881 ttatcggcga tttgtctgca caagaagttg aaaaagtaac cgcttccgtc ggtgatgccg
    14941 aagtggacca aactgtagaa atcctgcgta aacaacgcac ccgcttcaac catgtcgaac
    15001 gcgaagcccg aaacggcgac cgcgtcatca ttgactttga aggcaaaatc gacggcgaac
    15061 cttttgccgg cggcgcatcc aaaaactacg ccttcgtatt gggcgcaagt caaatgctgc
    15121 ctgaatttga agccggcgta gtcggcatga aggctggcga aagtaaagac gttaccgtca
    15181 atttccctga agactaccac ggtaaagacg ttgccggtaa aactgccgtg ttcaccatta
    15241 cgctgaacaa cgtttccgaa gcgactctgc ctgaagtcga tgcagatttt gcaaaagctt
    15301 tgggtattgc ggatggcgac gttgccaaaa tgcgcgaaga agtgcagaaa aacgtaagcc
    15361 gcgaagtgga acgccgcgta aacgaacaaa ccaaagaatc cgtaatgaac gcgctgctca
    15421 aagccgtaga gctgaaagca cctgttgctt tggtcaatga agaagccgca cgcttggcaa
    15481 acgaaatgaa acaaaatttt gttaaccaag gtatggctga tgctgccaac ttggatctgc
    15541 ctttggatat gttcaaagaa caagccgaac gccgcgtatc tttgggtctg attttggcca
    15601 aactggttga cgaaaacaaa ctggaaccga ctgaagagca aatcaaagcc gttgttgcca
    15661 actttgcaga aagctacgaa gatcctcaag aagtgattga ctggtactac gcagatcctt
    15721 cccgcctgca agccccgact tctttggcgg tagaaagcaa cgtcgttgat ttcgttttgg
    15781 gtaaagccaa agtaaacgaa aaagctttgt cttttgacga agtgatgggc gcgcaagcct
    15841 gattatcctg aaaatgccgt cttagggcag tttttgaaag caccgaagcc gcttttaaag
    15901 cccgctttgg tgcttttaca tcatgaaagg agacgaaatg tcttttgata actatcttgt
    15961 ccctaccgtt atcgagcaga gcggtcgcgg tgagcgtgca ttcgatatct attctcggct
    16021 tttgaaagag cgcatcgtat tcttggtcgg cccggtaacc gacgagtccg ccaatctggt
    16081 ggttgcccaa ctgttgtttt tggaaagtga gaatccggat aaggatattt tcttctatat
    16141 taactcgcca ggcggttcgg taacggccgg tatgtcgatt tacgacacca tgaattttat
    16201 caagcccgat gtatcgactt tgtgcttggg gcaggcagca agtatgggcg cgttcttatt
    16261 gtcggcaggc gaaaaaggca aacgtttcgc cctgcccaac agccggatta tgattcacca
    16321 gcctttaatc agcggcggct tgggcggtca ggcatccgat attgaaattc acgcacgcga
    16381 actgttgaaa atcaaagaaa aactcaaccg cctgatggcg aaacattgcg gccgcgattt
    16441 ggcagatttg gagcgcgaca ccgaccgtga taatttcatg tccgccgaag aggctaaaga
    16501 atatggtctg attgatcaaa tcttggagaa ccgtgcttct ttacagtttt aataagaaat
    16561 ctgagagaaa caaatgccgt ctgaaaacag ttcagacggc atttgtctta tagggactgt
    16621 attgggatga ttttgttctg gttgcgtttg gagccgtttt cgccgtagtt ggttttgaca
    16681 tactcttcaa caatagccaa aaattccgtt gcaatgttgt cgcctttcag cgttactttg
    16741 cgttcaccat ctacataaac aggcgcgacg ggtgtttccc ccgtaccggg caggctgatg
    16801 ccgatgtcgg ccaatttgct ttctccgggc ccattgacaa cgcagcccat tacggcaacg
    16861 ttcagggatt caaccccagg ataaagggta cgccatatag acattttttg gcgcaggtaa
    16921 ttttgaacat cttgtgccag ctcttgaaat acggtactgg tggtacgccc gcagccgggg
    16981 caggcggtaa ccatcggcgt aaacgaacgc aatcccatag tctgtaaaat ctcttgcccg
    17041 acgacgacct cctgagtacg cgggctgcca ggttccggag tcagtgaaat gcggatggtg
    17101 tcgccgattc cttcttgaag caagacggat aatgccgccg ttgatgcgac aatgcctttg
    17161 ctgcccatac cggcttcggt caaacccaaa tgcagcggat aggcgcaacg gctgcccagt
    17221 tcgcggtaaa cctgaatcaa atcctgaacc gcgctgactt tgcacgacag gatgattttg
    17281 tcttcgggca gtcccaatag aacggctttt tcggcagatt ccaaagcgga gacaatcagt
    17341 gcttccttcg tcacttcttc gggcggtttc ggcgcggaag aagcgaggtt ggcatccatc
    17401 atacgtttgg cgaggctttg atccaaagaa ccccagttta cgccgatgcg gacggcttta
    17461 tcgttttcag cagcagtccg aatcataaag gcaaattttt catcgccttt tacgcctttg
    17521 ccgacattgc cgggattgat gcggtatttg gacaatgctt tgccgcattc tggaaattcc
    17581 gccaacaggc gttcgccgtt gaagtggaaa tcgccgataa gcggtgtggc atagcccata
    17641 tcgtccaagc ggcggcggat ttcggcaact ttggacgcgg cttcggggct gttgacggta
    17701 atacgcacca tttcggatcc ggcatcgctc aattccttaa tctgcaatgc ggtggctttt
    17761 gcatcggcag tgtcggtgtt ggtcatagat tggataacga cgggtgcttc tgaaccgacg
    17821 gtaatatgat cgatgcggac ttgatgcgtc ttgcggcgtt ggagtgtgtt catatggttt
    17881 gaatctgttt attgaccggt gaggacggtt tgcaattctt ccgagtaggg gaaattcgcc
    17941 tgcaattgtg cttcatattc gtatgccgcc tgtgcgttgc cgagggcttt ggcaattttc
    18001 cagcctagca gcaaatcatc ggcctgaagg acttctaccc tgctttggta ttttttaaag
    18061 tagtaatcgg catcgcccaa ctgcccggcc agcattttgg tgcgcgccag ttctttaaat
    18121 gcgggtggga actgcggctg ggcggcgagg gaacgtttca aataggcttc cgccaatccg
    18181 aattgcccct gttttgcgct gcatatgcct ttattcaggt tggcaatata aggggtcggg
    18241 taggtggggt cggccagagc tttgtcgaaa tatgccatag attcggcagg gcggttgagc
    18301 ctgccgcata gaaaccaacc gtagttgttg ttgatttcgg cactgtcggg tttgatggag
    18361 agggcttgcc ggaaactttc ctgcgccttg tcattaactt tcaggtattg atagatttcg
    18421 gcacggacca gccaggcaag ctcgttttta gggtctgatt tcaaggcatc ttcaatactt
    18481 gccgtcgcct gacggtagtc ctgaccgcgc atatattcca ttgccaactg ggttttgata
    18541 ttggaaacct gattggcttt ttctgcccgc gaggggcggt aggaagtgct gcacgcgccc
    18601 aaggcaagga caagtaataa agagattcgt ttggatggct taaaaggcat aattacccct
    18661 gttgtccgat taaaatctgc tgccattttt gttggcggcg cgttttatcc tgaacctgac
    18721 ctgccaactg tccgcaggcg gcatcgatgt cgtcgccgcg cgtttttcgt acggtaacga
    18781 caaatcctgc ctgctgcaaa atatcgcgga acacacggat gttctcattg ctggagcgtt
    18841 cgtatccgga gtttgggaag ggattgaacg gaatcagatt gaacttgcag ggaacatctt
    18901 tgactagttc gatcagttcg cgcgcatgtt gcgccctatc gtttattccg tccaacatga
    18961 cgtattcgaa agtgatgaaa tccctgggtg ctttgaccag atagcgttgg catgcggcca
    19021 tcaattcttt caagggatat tttttgttca acggtacgat ttggttgcgg acttcgtcat
    19081 tggaagcgtg gagggaaacc gccaaagcca ccggcatgac atcgcgcaac ctgtccattt
    19141 ggggaaccat acccgaagtg gaaacggtta cacggcggcg gctcaaaccg tagccgtggt
    19201 cgtccagcat gatgcttaag gcggtaacga cattgtcgaa gttcgccatc ggctcgccca
    19261 tacccatcat cactacattg gaaattacgc gttcgttttt cggtgtaaca cccatcgctt
    19321 tgtttgccca ccacaactgt ccgatgattt cggcagcagt caggttgcgg ttgaagccct
    19381 gccggccggt cgagcaaaat gtacattcca aagcgcagcc gacttgcgag gaaatgcaga
    19441 gcgtgccgcg atccgattcg gggatgaaga cggtttccac cccgttcccc gtcccgacat
    19501 ctagtagcca ttttcgagtg ccgtctgaag attcttgaga catcatcagc ttgggaattt
    19561 cgatgcttgc ctgttcattt agtttatggc gcaacgattt tgccaaatcg gtcatttcgt
    19621 taaaattttg cgcgccggat tggtgcatcc aacgcataac ctgtttggca cggaagggtt
    19681 tttcgcccgt atcggcaaaa tgtcgggtca gcccttgaag gtcgtagttg agcagattgg
    19741 ttttcatgtg tttgtgtttt tcttaaatca aggcttcaaa taaaaaattg cagggcaggc
    19801 aagtttgatt tgaagccttt tgcaacagag ttttcagacg gcatggatat atttatgccg
    19861 tctgaagagg agttgggcgg tgtattgtat caacgggggc agatttcggt ttggctgaaa
    19921 aagtaggcaa tttccaaagc ggcattttcc acgctgtcgg aaccgtgtac ggcattaatg
    19981 ctgaccgaag tggcaaagtc cgcgcgtatc gtgccttcgg cggcttcgga aggattagtt
    20041 gcacccatca gttcgcggtt tttcaggacg gcgttttcac cctctaatac ctgaatcata
    20101 accggaccgc cggtcataaa ttcaaccaat ccggcgtaga aggggcggtc tttatgaacc
    20161 gcataaaatt cttgcgcctc tttgagagta agctgcttca ttttggcggc aacgattttc
    20221 agaccgttct cctcaaagcg gctgtatatt ttgccgataa catttttgcc gacggcatcg
    20281 ggtttgatga tggagatggt acgttcaatc gccatgctat atccttattt ttacttaaga
    20341 agaatcaaat cggtattcta tcaaaaaaat aacttatccg cctgaagcct tgggagatag
    20401 ggtcaggtgc ggctgttcca aataaacttc ggactgcatt tcgaccatcc ggctggcggt
    20461 gcgggtaaat tcttcggcaa aatcgccttc cgtatagata tggttgacat ctaccgcgcc
    20521 ggtggcacac agtttgaccc ggaaatcgta gagtacgtca atcagccaag tcagccgccg
    20581 cgcctccgcc ttttcttgcg gtgagagttg ttccaaacct gaaataaaaa ccatttcata
    20641 atgttcggcc aaatacagat agtcggactg tgagcggggg ccgaagcaca gtgcgcggaa
    20701 atcaaaccat atggcacggc cggactcggc tttgtgggga atctcccgac cgtggatggt
    20761 gctgatgccg gggttcaaat cggtaatgcc tgtcatttct ttgaacagtt ttgccagttt
    20821 tgcctcattt tcttcattgg caggcgtaaa gaaaatctcg gcggggcgga gggtacgcag
    20881 tcggtagtct tcaccgccgt caacgtttaa gacggtcagg ctggactcga tgagcgcgat
    20941 tgtgggaaga aaactgctcc ggttttgacc ttgcgggtag agttcggaag gcgcgtagtt
    21001 tgaagtcgcc accaaaacaa cgccctcgtt aagcaggttt tccagcagac ggcctaaaat
    21061 cattgcatcc gcaatatcgc tgacatgaaa ttcgtcaaaa cacaatacgc gggtttcttt
    21121 ggcaatctcg gcggcaacgg atttcaacgg gttgctttcg cttttcaggg ttttcagcct
    21181 ctggtggatt tctgccataa aggcatgaaa gtggacgcgg cgtttgcggc ggtaagggag
    21241 gcagccgaaa aaagcgtcca tcagaaagct tttgccgcgt ccgaccccgc catagaaata
    21301 aagccctttg gggacttgcg gggaacgcaa actcctgcct aaaaaacggt ttcttttgcg
    21361 tttgaacatc atcaattcgg tccaaagccg atcgaggtgt tcgatggcgg ctgcctgcgc
    21421 gtcgtcgcgg atgaagttgg gcagttgtga ggcagcctga taccaggtca gcgggctgtg
    21481 gttttcaaac ggcggggctt taaaaagttg gtctctatta ctcatattta accttgttat
    21541 acttcttgcg gcggagaaag aaaatgctta ccgtcggtat tataaatgat ttccttcaaa
    21601 cttggcattt tccgtggaaa agaataatga cgcgcatcag caaaggcggc gttccaatat
    21661 gatgttgata tcgccaattt attgaaagaa atgccttttg tttcaggctg cgttttaata
    21721 ttgaaggagg acaaatgaaa cagatggtga atcgcgaagc tgttacgcca ccgtcagctt
    21781 ggagggggag aaaacacaac aggctgctta tgtaaattcg acaatgaata atacgaagcc
    21841 cgaaagtgaa gtaggaaata cgggtgattt taatgtttga ccaatcaggg tttaagctta
    21901 aaaatcatac taatcaaatc aagggcaggg tttggtaatc cgtagggtcg gctccttccg
    21961 gttcagacgg catacgttta tgttggtatt ttgccggcac ttcttaccgc cctcgtgccg
    22021 gctatcccaa aactgctttg aacactgaac gctcttcggt ttacttcaaa aacgcggcgt
    22081 gataagcaat atgctcgcca ataaaactgg cgatgaagta atagctgtga tcgtagcctt
    22141 tatggaaacg cacatcgacc ggctggtttg ccgcacggca ggtttcgata aaatcttcgg
    22201 tacgcaattg tgtcggcaaa aactcatctt ccaagccttg atcgatgcgc ataccttgca
    22261 ctttatagcc ttgttgaatg agtgagttcg catcatattg ctgccatttt tcgcgatctt
    22321 ttcctaaata agcagtaaag gctttttctc cccacggtac gaggcttggc gataaaatag
    22381 gcgaaaaggc agaaacactt tgataatgtt cccgattccg tagtgccaat accaatgcgc
    22441 cgtgtccgcc catcgaatgc cccataatgg aacgtttgcc gttggtagga aagtgtttct
    22501 caatcagacg gggcagctcg ttcaaaatgt aatcatacat ttgataattc gccgcccaag
    22561 gctgttcggt cgcattcaaa taaaagcctg caccctgtcc taaatcgtaa gcatcatcgt
    22621 tcggcacttg ctctccgcga gggctggtat cggaggccac cacaattact tgatgttctg
    22681 ccgcataacg ctgaaagcct gatttggtaa tgaaattttg ttccgtacac gtcaagccgg
    22741 aaagccaata aatcacacca agcggtcgat tttctggatt atttggcaaa tagacggcaa
    22801 atttcatttc gcattgcagc gtttgggcat gatgcgccca aacttgttgc gaaccgccaa
    22861 aaatttgatg ttgttcaatc agtttcatcg cataccttag tagtgaataa cggcgcggat
    22921 cgatttacct tcgtgcatta actcaaaggc tttattgatt tgatcgagtg tcattgtgtg
    22981 ggttacaaac ggttctaact caatgtcgcc tttcattgaa tcttccacca ttttcggaag
    23041 ctcggagcgg cctttcacac cgccaaatgc tgaacctttc caaacacgac ctgttaccaa
    23101 ctggaacgga cgcgttgaaa tttcttgtcc tgcacctgct acgccgatga taatggattg
    23161 accccaacca cgatgtgcac tttctaatgc ttgacgcatt acgtttacat tgccgataca
    23221 ttcaaaggta tggtcaatgc cccatttgtt gatgtctaac aacacatctt taattggttt
    23281 atcgtaatcg ttcgggttca aacaatccgt tgcaccaaac tgttttgcca actcaaattt
    23341 tgcgggattg gtatcaatgg cgatgatgcg gcctgctttg gcttggcgcg caccttgcac
    23401 cacagccaaa ccaatcgctc ccaagccaaa cacggcaaca gagtcgcctt cttgcacttt
    23461 tgccgtatta tgcaccgcgc caatacctgt ggtaacgccg cagccgagca aacatacttg
    23521 ttcatggttg gcttcagggt tgattttcgc cagtgaaact tcggcaacaa cggagtattc
    23581 actgaaagtc gaacagccca tatagtgata aatcggctga ccttgataag aaaaacgcgt
    23641 cgtgccgtcc ggcattaagc ctttaccttg tgtatcacgc actgagacgc acaagttggt
    23701 tttacctgaa caacaaaact cacattcgcc acattcggcg gtgtaaagcg gaatcacgtg
    23761 atcacccggt tttacgcttg acacaccttc gcccacagca acgaccacac ccgcaccttc
    23821 gtgtccaagc accacaggga atacgccttc aggatcgctt cctgataacg taaacgcatc
    23881 agtatggcac acgccagtgt gggtattgcg gattaacacc tcgcctttac gcggcatttc
    23941 tacgtcgatt tccacaattt gtaagggttg gttaggggcg aatgccaccg ccgcacgaga
    24001 tttgatggtt gaatcggttt gtttcatttc cattgtcttt cctcttaatt cattgacaaa
    24061 gttgccttac ctgagcaaca tagcaacagt ttacacttta gagttaactc taaagcaagt
    24121 gttttttact gtttttgttt agaaggaggg aaaatgactt ataccactgc caaagccgcc
    24181 gaaaaaatag gcatctccgc ccacacccta cgtttttacg acaaagaagg tttgttgcct
    24241 aatatcggac gtgatgaata cggtaaccgc tgttttaccg ataacgattt gcaatggttg
    24301 ggcttattgc aatgcttgaa aaatacgggg atgagcttaa aagacatcaa acgctttgcg
    24361 gaatgtaccg tcattggcga cgataccatt gaagaacgcc tttccttgtt tgaaaatcaa
    24421 atagaaaatg tgaagtgtca aattgccgaa ttaaaacgct atttagattt gcttgaatac
    24481 aaattggcgt tttaccaaaa agcgaaagca ttaggttcgg taaaagccgt aaatttgccg
    24541 caaattcctg aaacggctta gttttagtgc aataagcagg atttttgtcc tgttcgtaat
    24601 agaaatcaaa aatttgttgt ttaaaaagcg gattgtaagc aatgatatag tggattaaca
    24661 aaaatcagga caaggcgacg aagccgcaga cagtgcaaat agtacggaac cgattcactt
    24721 ggtgcttcag caccttagag aattgttctc tttgagctaa ggcgaggcaa cgccgtactg
    24781 gtttttgtta atccactata aaaaaatcca cgctctaatc cgttgcttgc ttggctcaag
    24841 gtattgaagc agcggatgaa gttaaaccac agaaaaaacg ccgcgtaaca gcggcgtttt
    24901 gatgctaagg tttaattggc attttctttt aaagccaagt cgacccgctc gcgcaattct
    24961 ttaccgggct tgaagtgggg tacatgtttt tcaggtactt ccacacgctc gccggttttg
    25021 ggattgcgac cgatgcgggc aggacgatgg ttcaaatcga agctgccgaa accgcggatt
    25081 tcgatgcgtt gacctcgggc aagcgatcta gtcatggtgt caaccaagac ttttacgctg
    25141 tactctacgt cttttgccag aagatgcgtg ccgtttttgg cggcaaacac ttctgccaaa
    25201 cgaaccatta actcagactt tgtcatgtct gcaaccttat tcttgttcgc cggagagttt
    25261 ggctttcagc aggtcgccca agctggtggt gccggcattc gcattggcgg cggcattgac
    25321 ggagttcagt gcttcgcggc tttctttggc atctttggct ttaacggaaa gtttgatgct
    25381 gcggtttttg cggtcaacgg taacgatgac ggcttcaact tcgtcgcctt ctttcagttt
    25441 ggtggtcaaa tcttcaacgc ggtcggctgc aaattcggaa gcaggcaggt agccttctac
    25501 ttcgtcagac agggcgataa cagcaccttt ggcgtcaaca gatttcacgg aacctttaac
    25561 caaagaacct ttgtcgttca cgctgatgaa gttgccgaac ggatcgcctt ccagttgttt
    25621 gatacccaag gagatgcgtt ctttttccac gtcgattgcc aatacgacgg cttcgacttc
    25681 ttcgcctttt ttgtatttgc gtacggcttc ttcgccggat tcggtccagg acaggtcgga
    25741 caggtgaacc agaccgtcga tgccgccggg caggccgacg aatacgccga aatcggtaat
    25801 ggatttaacc gcaccggaga ttttgtcgcc tttgttgtgg ttggcggcaa attcttccca
    25861 aggattggct tggcattgtt tcatacccaa agagatacgg cggcggcctt cgtcgatttc
    25921 caaaatcatg acttcgactt cgtcgcccag ttgtacgact ttgctcgggt gtacgttttt
    25981 gttggtccag tccatttcgg agacgtgtac caaaccttcg atgccttgtt cgatttcgac
    26041 gaatgcgccg tagtcggtca ggttggatac tttgccgaac aggcgggtgc cttgcggata
    26101 acggcgggtc agaccgctcc aaggatcttc gcccagttgt ttcataccca aggaaacgcg
    26161 ttgtttttct tggtcgaatt tcaatacttt ggcttcaact tcctgaccga cttccaagac
    26221 ttcgctcggg tgtttcacac ggcgccatgc caagtcggtg atgtgcagca gaccatcgat
    26281 gccgcccaag tcaacgaatg caccgtaatc ggtaatgttt ttaacgatgc ctttgatgac
    26341 ggagccttct tgcaggtttt ccagcagggc tttgcgttct tcacccaagg tagcttccag
    26401 gacggcgcgg cgggacacaa caacgttgtt gcgttttttg tccagtttaa tcactttgaa
    26461 ttcgatctct ttgccttcga agtgagaagt gtcttttaca ggacgtacgt cgaccaaaga
    26521 acccggcagg aatgcgcgga tgctgctaat cataacggtc aggccgcctt tgactttgcc
    26581 gttgatgatg ccggacagga tgttgccgtt ttccatggct tcttccaggg caatccaatc
    26641 ggcagcgcgt ttggcttttt cgcgggacag tttggtttcg ccgaagccgt tttcgacgga
    26701 ttcgatggta acggtaacga agtcgccgac tttaacttca atttcgcctt gagcgttttt
    26761 gaattcagct acatcaatca gggattctga tttcagacct gcgtttacgg taacgaagtt
    26821 ttggtcgatt gccactactt cagcggtaat cacctcaccc gggttcattt cttgcagggt
    26881 aaagctttct tccaacagct gagcaaaatt ttccatagac atatataact cttttcggta
    26941 caccgccaag gggtgcgggg tgggttggtg gatgtctgcc gtccttggca gggcagacgg
    27001 gtaaataagg tttcagacgg catatgggga aattcatgct gtctgaaacg caatgcgggc
    27061 gattatacct gaaaatttaa actttacgat accaatcaag cacttttttt acagtttctt
    27121 ctatagtcag gcggctcgta tccaaaagca gggcatcggg ttgttgtttc aggggggcaa
    27181 ccttgcggtt tcggtctgcc tcgtctctgg cttcgatgtc ggacaggatg cgctcgaatg
    27241 ccaaaccttc gcaggggatg ccgatttgtt tggcgcggcg ttcggcacgg attttggact
    27301 ctgccgtcag gaagatttta agttcggctt gggggaagac gaccgatccg gtgtcccgtc
    27361 cgtcggcgac cagtcctttt tcggtcagaa aatcgcgttg gcgttgcagc agggcggcgc
    27421 ggactttagg caactgtgcg actgcggatg cgcccatgcc gatggcttct gtccggatgc
    27481 cgtctgaaac gtcttcgccg ccgagcagga tgcggctgcc tgaaaatacg gcgggcagtt
    27541 tttttgccag ttcggaaacg ttttcttcat cgtgccatcc cacgccttgt ttttgtgcat
    27601 atagggcagt caggcggtag agtgcgccgg tatcgagata atcgtatccc aatgcggcgg
    27661 caacgcgggc ggcaaccgtg cctttgcccg atgcgcccgg gccgtcgatg gcgatgactt
    27721 tttgtctgtt cataaggggg attcctgatg gtttggggta tgggttttgc cgtctgaagg
    27781 atgtgtttcc cgttggggcg gattctacct gttttaaagg acgattgtct aagcagacgg
    27841 aacgccgcct gcccgaaaac atccgacaga agccggcaag ccggttcgga ttccggcgtt
    27901 gcgggtattg tggcgggcat caaggcaatg ccgtctgaaa gaggtatggc cttcagacgg
    27961 catcgattgc tgcagattag aacaggttgc tgacaaaaat cagcaaaatc accaacggca
    28021 caagatattt cacataagca aaccaaatat tgaccgtcgt atggttgcct ttgtaaagca
    28081 attcttcctt cgcttcgtcc ttcatcacaa aaccgacaaa cagcgcgaaa ccgagcgcgg
    28141 tcagcataaa caagatgttg ccgctgatgt agtcgaaggc atcgaaaata tttttgccga
    28201 acacggaacg tctttccacg aaccatagct cagaatggac gggatgttgc cgaaaatgaa
    28261 gatggcggct aatacaatcg taatcgcggc ggtacggcgg attttggttt tttcctgaat
    28321 ggtcgtaatc aacacttcat aaatggtcag cgaagtcgtc aatgcggcaa tcaggagcag
    28381 cgagaagaaa atcacggcga acacagatcc tgcccacata tgcgagaaca caatcggcaa
    28441 actttggaat accaaagtcg ggccggaatc gggggcaacg ccgaagctga agagcgacgg
    28501 gaaaatcata aagccggcca gtacggcgat gatggtattg gtaatggccg tgatgaccgc
    28561 ggtttgcacc agattttcgt tcttatccag atagctggac aaggtaatca tcacgccgaa
    28621 acccaagctc aaggtaaaga acacttgtcc taaaacgaag acgaacagtt cggctgtaat
    28681 tttgctgaag tcaggtttca gatagaaggt aatgccttcc attgcgcccg gcagggtaac
    28741 gttgcgcaca accatggcaa tcaggaataa aaacagcaaa ggcatcaggt attttgctgc
    28801 tttttcaata ccgccgatca cgccttttac caagatccat tggttcacaa ccacaaatag
    28861 gaaggtgtag aaagcaattt cccaaggact gttttcgatg tgttcggtaa agaaactttt
    28921 ggtgacttcg ctggaaacag gactggaaat gtccaaatta ccgccgatga tattaacaat
    28981 gtagctgata acccagccgc cgagcaccat gtaataagcc atgatgccga acgcgccgag
    29041 cagcccatcc agccgaccag tttccaaatt ttggcaatgg gtttgccgtt catcgggccg
    29101 ccgaacgcat ccagcgcgtt cacgcctttg cgccgtccga tgacattttc caccaaaatc
    29161 atcgggatgc cgataaccag catcgcgata cagaataaaa acacatacgc gcaccaccgt
    29221 tttcaccgac caaatacggg aaacgccacg tcgcgccgaa accgacagtc gcgccggcaa
    29281 cggtcaggat aaaggcttga tgttgggtaa gtttgtcatc atcggtgacg gatttggctt
    29341 tgttggcgat gaccaagccc aaatggtgct ggcgagatag gggctgtaag gtggggtgtc
    29401 gtttcatggg ggttcctgtt ggtaaattaa attttattat aaaataaaaa aaccacaaac
    29461 actagggcga tgtttgtggt attgtggcac ataatgctga tcaataattt aaggtataac
    29521 ttatcccata acttctcccc tgtgcagaca agctggataa ttcaccataa gtcactttgg
    29581 cagcttgact aaatacactt ttatattttt tatttgtcaa gttataaatc ccaaagttta
    29641 actgaccttt aggtagtttg atctcaccca ataaatccgc cacgacatag cctgaaattc
    29701 tagcctccgg ggttttgcgg accttttgat tggttttggc aatcaggctg tccttgtaag
    29761 ctctttgtgt accatcaatg gcaagtagtt gtaagcgtaa cttatgttca cttggtgtgg
    29821 tgtattgagc aaaagcggtg gctttggtag ggctgatacg aaacgcccca agttctcgat
    29881 attgaccgct tgcatctttg tattgacctc gtgtataggc aatgctacca ccgacagccc
    29941 agttatcatt aatcttgtga tttaattcaa actccgcccc ataaatacgc tcatcagtat
    30001 cagcaaccac cacgctataa tcttttaaaa actgggttac tttatctgat gtattataaa
    30061 aacctgtcaa cttggcacgg ctaccgccaa acactcctac ccaccctgca tcataactat
    30121 tgactgaaat tggctcaaca ttatcagaat taaccgcaaa acccgtccca acatctctta
    30181 gcaccctttg taaatcaggc aaactatatc cttgcccaaa attggcaaaa atactgtgat
    30241 ttggagttaa tgagtaattt gctccaagat taaataaggt tttgtgatgc ttcactttgc
    30301 cagaattgac agttcctgct tgatacggca ctttattctg gtttaataaa tcggctaata
    30361 gcgactcact gggtggcaca aaaccatctg ttttactgtc tataatttga tgccgtacac
    30421 ctgccgatat ttttaaatca tctgtcaaag caacattagc attggcaaat atgccgtagg
    30481 tttttaaggt ggtgtcaggt ccataagtgt aacggttgcc agtgtcttta aatgccaagc
    30541 cgttgctttg cataaaggta gtcaaatcat agcctgtcgc cccttggcgg tttttttctt
    30601 gttcataatc gataccataa ctaagtagta caggtttttg agcaaggtca aattttcttt
    30661 gcaaagccag tcttgctcct agtacctctg ccttagattc agattgtaac accccatagg
    30721 cgcgataagc atccaaagtg gctggtggca aattcatgct ttgtaaaata ggcagggctt
    30781 tggcggtcga aaacggggca acaaagggat aaaatctgcc tttctctctg cgataataag
    30841 cattggtatt gatggtgtta ccccacaaat catcatgatg atagttgata ttaaaggtgc
    30901 ttttggtggt ttttggctgt tctgataatg ataagccttt gatggcattt aatgaaggct
    30961 tttcttcaaa caacaccgca aggcgattac cataatcagg tgcataatcg gtgtcttgtt
    31021 tgtcgttata atgcgtcaat gccagattga tattttgctt gtcgtcaagt tgccaatcaa
    31081 catttgtatt gacacttagg cttttgctgt cttgcttatc agtttgggca ggctcaggag
    31141 cgatgcgttt accgttagca tcaaatgccc ctcctgtggt gcgatagtcg acatcaagtc
    31201 gtgcaaggac attaccattt tcgctgacac ctgctacact ctgacctacc tgataaccga
    31261 taccttcact ggataattta ctaccatgta caccgatgcg ggtttcaaat tgctcctctt
    31321 ccaaatcaga cttagtaacg atattaatca aaccgcctgt tgctccagac ccataaatac
    31381 tggttgctcc tgataaaact tcaattctag caacttgatt gggattgata ctattaagct
    31441 gtctagagat gtctcgcgaa cctgtcaaag gcacgccatt taacaaaaat tgcacttgac
    31501 gaccgtgcat ggtttgccca aagttactgg tagtgccact acttaccccc aaacttggaa
    31561 tgagctgtgc catcacatcg gcaagctgtc gagagcctgt tgcttgctct tgaatttgct
    31621 cttcataaat aactttggtg gtatggggca tttgcgtgat acgattggca agtgcatacc
    31681 gctcttatca atggtaataa ccacaggctc taattcaacc gttggtaatg attcattggg
    31741 ttgagcgtac aactgctgag ttactgccac acttagtaaa ggcaaagata accattgaaa
    31801 atagtgggaa tgacgcataa attactcctt atggaaaagt aaaattttaa cagtctgtta
    31861 cggtttaaca gtctgttacg gaaaattttt ttatcaatgt cggcaaaata agcattgaca
    31921 ccccagaacc aatcgcaagc cacaaaaata gcttgtcata gccaaactga tgtactaaaa
    31981 atccactcat cgccccgccg ataaaataca caagcaactc cacgcacaca aacaaagtaa
    32041 agtccgtgcc agcttggtcg ggattggcag cattcataaa cagagcatat aaggcagtca
    32101 ttgttaaata gcgaacagat aaaattagca aaacactaat tgccatcaag ctcataaacc
    32161 ctgtttttaa taaacctacc gcaggtaaaa aatcacccaa taaaggatat agcaatccta
    32221 gacacaacaa agcataaact gccgttctta gccatccgaa taatataaga caacgtacaa
    32281 tgtcgatata tttgattaat acccctgcca aaagtgctga accaatccca acaatcgctc
    32341 caaataccga aaacaaaagc ccaatctgag acagtgaaaa accaaaatcc acaagcattg
    32401 gatttagcat tgccatcatc ggagcttcca caaagcgaaa caccacaatc aaagctagta
    32461 accatttagt ttgcgggcgt ttgataagcc taataagact tgctctggaa agattggtag
    32521 catgtttggg attgtcttga tcttttaaga accatagtga acacgaaata agcacaacag
    32581 ttgttgccaa actgattgtc gccatttgcc aatcaaatct ctgatatagc cacagtgtgc
    32641 cagcaccgcc taccatacta ccaaacgcca ctccaaaact ttgcatggca cttgccagcc
    32701 caaaggatga cttatcaaat tgttccactg tatagccatc aatagcgata tcttgggtgg
    32761 cacttgccaa agacaccaaa aaacttatgc tcagaaataa tacaatctca gactcagaaa
    32821 actgtaaaaa gccaagccca aacagcccga ctaccacgat aatttgagta aaaaataacc
    32881 aagttttgcg tttgccgatt ttggcacaat aataattgtc cacataactt gcccacagaa
    32941 atttaaacgc ccacggcaaa tataaaagtg aaagcatgcc aatcacccta atatcaacgc
    33001 cttgatgtct taaaatggca ggcaatgcca cattaaaaaa atacaaaggc aaggcatggg
    33061 caacatataa cagtgccacc atcgtcaaaa gatggcgatt tttttgttgt gagataatca
    33121 tcagaccgcc cctattttag tgtttatcca tgctttggct tcagtcagtg acaacaccac
    33181 cgcataaggc actcgccatg ctttatgaaa agcaggactg tctaaggctt gtctttgagt
    33241 ctcatcatca acaattctga ctaatccctt gcaatattta aagaacgatt ctttgtgcgt
    33301 cttgtaccaa accgcctcaa tctggcggta gttattaggg agtgtcagcc ctgtccgact
    33361 atggtaaatc aaggcaaaac tctgctcact ggctaacaaa tcactcatag caatcaacca
    33421 atcatcaaat gcatcttgac taatctcgcc tgaaaaatcg agtaaaaata caggtaagga
    33481 gctatttggg ttctgaatag catttagaaa aataggcata acatcactct ttaatgctgg
    33541 catttaaata atgggcaaac ttacaataaa acccttcttt gtcacaaaaa tataacattg
    33601 cagttttttc aggcattaca atttgatatt ggggcatata gtttaattct tgagcgacta
    33661 cctcataggg ttttacactt gcatcaggtt cacccacaat ttttttagca ggtgaatgtt
    33721 caaaaataaa aaagcttagt agacgcatga tggcacgcaa gtaccctttg ccaacagcct
    33781 gtgcctctgc tagtaacaaa tgccaaccca aatcattttt gtcagctgta taataccctg
    33841 atagtctatc ccttgcccct tcataaactt cggcgtaacc aacccattcg ccattaatac
    33901 taaccaaata caaacgctgg tgatcatcag ccaacatttt ttcaaaataa acagacagct
    33961 cagacaatga tttattgagt tgccattgag ggataacatg gggcatattc atccaacgat
    34021 gcaacaattc aatatgttca gggtagcgta tttgcaccaa cccaaattca accccatttt
    34081 ctctgtaaat atagttgttt ggcaacccct gagataaagt tggcaatcga ttgattttac
    34141 tgcattgatc ttgattaaaa gaatcggtat agatttctag catttctatt ctctcattag
    34201 ggtgtgttga atatttggta tttgcaatga aaaatgagaa aaatcgcacg tttttcatgc
    34261 aaaaactaat aaaagcgtta aaattttcat catttttaca aataatgtta tcaatgttca
    34321 acacacctta gaaactgtgg tgatattgga ttgcaaattg gttcgcaaac cacgggttct
    34381 ggacggttgg cgttatcttg ataacctctt gtaaaaaagc gaacacgatt taggcacacc
    34441 ttttcaaatt ttggtctttt taaatcaaat aaagcaatcc tctcatttag ctctggatgt
    34501 tgatggtgaa attcatcaat aatctttgat aactcattaa ttgatttttt gcgttaaatt
    34561 gggttaaacc atatatttaa tgagtatgtg gatgaatata aatggaattc ttggatgtta
    34621 tttcagaatt ttggacattc gataaatttc cattccccgg gtttgaggtt ttctgtttcc
    34681 agatgtgcga atcgccggcg atgaaggtgt tgcacgcggt tgccggcggc ggcgatcatg
    34741 cgtttgactt ggtggtattt cccttcggta atggtcagca gcagggtggt cgggttttcc
    34801 aaaacggcat cagcggcgca aacggtttcg ttttcgtcgt ggagcagcac gccgtttttc
    34861 aaggtttcgc agagcgtttc tcctgtgggg tgtttgagcg ttacttcgta tagcttggga
    34921 atttttctgc tcggcgaagt caggttgtgg ttcagtttgc cgtcgttggt aatcagcaat
    34981 acgccggtcg tatccgcatc cagcctgccg accgcctgca tatcgatgtt ccgcatattg
    35041 tcggggaaca ggctgaatac gctgcggtag tgcttgggtt tgtgcgaagt ttcgtaatct
    35101 tcaggcttgt tgagcatgat gtagaaatag ggttcgggaa cgacggttac tgcttccccg
    35161 tcaatatcca acgtttcgac ggatgaggaa tcgatgtctg catcggtgtc gtccatacag
    35221 gttccgttga tggagacgta gccgccccta atcagccatt ggcactgctt tcggcttcct
    35281 atgccttgat attgcaggta tttgatgagt ttcatgatgg tatgggaaat gtgggatgaa
    35341 aaagacagga ctgagaaagt cctgtcatgt gccgtatccg aatcagcttc caatcagttt
    35401 aaccctctgt cctggattga tggtgttcag gttgggattg agccggcgga tgtcgtcgat
    35461 atggatattg aagcgcgcgg cgatactttt gaaggtgtcg cctttgcgcg cggtgtagga
    35521 tactttttca atacgggttt gggcaggggc tgcctgcgcc aggcggagta cctgtccttt
    35581 ttggatggtg ttgcctttga tgttgttggc gacaatcagg tcggctacac tgacgttgta
    35641 gcgtttggca atgttgaaca gggtgtcgcc ttctacaacg cggtggatgc tggcatggag
    35701 cggggagatt tcggcttcgc cgtcggcagc tttggctacg cgcgtttcca aacgtacccg
    35761 gcgtttgccg tcggcggcaa cggtatgttg cggagatgag gaaatgttgt gttcggcaac
    35821 tgtgtcaggc gtgccgatga cggcggagat ggtttcttca gcctgtcggc gcaggttgtt
    35881 tcgggcaaca agctgcatga gttcgtctgc cgcctgcatg tcgttttggg ggacgatctg
    35941 cgcgagtgtt gcggtttggg tttcagacgg catggcggtc tgtttttcgg tttgaggttg
    36001 cgctgcggct gtcgcaaggg caggttcggc aatacggaca aggggatcgg gttccgtacg
    36061 gacggttttc tgcggcaaag gtgcgacggt aatgtccgct gtctgtgcgg cggcgggtcg
    36121 gattcgggca atgccgacgt tcaccgtgcc tgccggcata ttggaacggt aggtgtcggg
    36181 tgtattgtcg atgtcgatgg aaacgacgga ttccgatgcc gtctgaaggg ttttgccgtt
    36241 cttggcgaca aggatgctgc gtcctgcgtt gacaaggttg ccgttcaggt tgttgaggcg
    36301 tttgatgtcg gcaatgctca tgccggttgc cgtcgagatg tcggacaggc tggttttggc
    36361 ggcaggcgta tagacttccc atgaaaacag gctgtcgggt gcggcgttga ggtagttgct
    36421 ttggaaggtt tgtacggacg cgacaggaag cagcagtttg cgtttgcttt tggggataaa
    36481 cgcggggacg ttgaatgcgg ggtttagggc gagcagctcg ctttgcgtga tgccggcaag
    36541 ccggacgatg gcttcgttgt cgagcggacg atccggttcg actgcctgaa aatagggttt
    36601 gttgtctatg tcgctgatat tcatgccgaa agattgggga gtggcaataa tgttgcgcac
    36661 ggcgagcagc ttggggacat agttgcgcgt ttcgttgggc atacgcaggt tttcgtaggt
    36721 cggttcgagc ccttgggcgc gggcgcggtt gatggcgcgt ccgacgttgc cttcacccca
    36781 gttgtaggcg gcaaaggcga gcggccagtc gccgaacagt ccatagaggt attgcagata
    36841 gttgagtgcg gcatcggtgg cggcgtaaat gtcgtgcctg ccgtcgtaaa ccggtgtttt
    36901 ttccaggccg taatgcctgc cggtagcggg cataaactgc cataatcctg atgcgccgac
    36961 gtgtgatttg gctttggtga cgaacgcgct ttcgatgaag ggaagcaggg cggcttcggc
    37021 gggcatattg cgttttttga cttcgttggc gatatggtac atatagggtc tactccggtt
    37081 gatgaccctg ttgaaatagc tgtggcttgc gatgaatttg ctttcgtggc ggcgtaccag
    37141 ttcgggattg acttcgccca tccggaagcc ttggcgcagc tcgctccaca ggctgccgga
    37201 ttggaaatat tgttttgtcg ggggcaggtc gagtattgaa gagtttaagc gcataatcgc
    37261 caaaccgatt tggtgtgatg aggtgttttg ggcgtatagg aaacccggac aaacggacag
    37321 acctgatgcg gtcagagcga tggttttgag tttggacatg atttatattc ggcggaataa
    37381 acatatttta tcgtccgatg gtactgtttg actgaaaaag cgtcaagttt tacaggaact
    37441 ttatgcggat tcggaaatgg gcggccgatg tgttttttga cagaaatcct gtttggaata
    37501 gaatatcggc aggcaatctg tttgaaacgg acgcaatatg gattcatggt ttgaagatac
    37561 ggcgatgggg cggtatgttg caaaattgga acaggatttc ttcgggcggt atctggattc
    37621 ataccgtttt tcgggaatgt gtgcggttca ggtgggcggt ccgtggctga gcctgtctga
    37681 agatgttgtc tgtgtgccgc gagatatgtc gatgtcggcg gaaaatatgg ctttggcgga
    37741 tgtttctgcg gatatgctgc ttttcccgca tactctggaa ggcggtgttc cttcgcaaat
    37801 cctgtcggaa gcgcaccgga tattgaaacc gtgcggacgt ttgatgctga cgggtttcaa
    37861 tccgtattcg ctctggggat tcagccgttg gtttgacggc gaacgcctgc cggaaaaacg
    37921 gttttgtctg ccgctgcccg agttgaaaag gcagcttgcg gatgtcggtt tcgatattga
    37981 atttgggaaa tttatggtgt atctgccgcc cgtttcgtcg ctcgggcaaa tacgcttttg
    38041 gcggtttatg gaaaaggcgg gcgaccgttg gtggccgcag tgtgccgcag tgtacggttt
    38101 ggttttggtc aaaagggcgg cgggcgtaac gcccctgccc gcgtgggacg ggtatttggg
    38161 cggcaaagcc cttgcggcgg gtgcggcaag gattgcggat tagggacagc cgtctttcag
    38221 acggcatctg tcagaagaaa tgtcgggagg ggttatgcct gaattgccgg aagtggaaac
    38281 gacgttgcgc ggcatcgcgc cgcatattga agggaaaacg gtggaagccg tggtattgcg
    38341 ccaattgaag ctgcgctggc agattaatcc cgatttgggg gagattttgt ccggccggca
    38401 ggtgttgtcc tgcggcagga gggcgaaata cctgcttatc cgctttcaaa cgggcgtgct
    38461 gctgattcac ttggggatgt cgggcagctt gcggattttt acgccgtcgg acggacgtat
    38521 cggcaggccg gacagacacg atcacgtcga tattgtgttt tcagacggca cggtcatgcg
    38581 ttaccgcgat ccgagaaagt tcggcgcgat actttggtat gagggaatcg aagaacatca
    38641 tccgctgttg gaaaaactgg ggccggagcc tttgtcggag gcattttgca cggattatct
    38701 gtatgtgagg ctgaaggcgc agaagcgcgc ggtcaaactt gccctgatgg acaatgcggt
    38761 cgtggtcggt gtgggcaata tttatgccaa tgagagcctg ttcagagcgg gcatttcgcc
    38821 ccaccgtcct gccaaccgtc tgaaaaagaa agagtgtgcg cttttggttg aaaccgtcaa
    38881 agcggtgttg cggcgcgcca ttgaaacggg cggcagtacc ttgagggatt ttatggacag
    38941 cgacggcaaa agcggctact tccaacagga atatacggtg tacgggcggc acaatcagcc
    39001 gtgcccccag tgcggcggtt tggttgtgaa agaaactttg gggcagcgcg gcacgtttta
    39061 ctgcccgaat tgtcagaaat agggcggaaa acggtttcag acggcatttt atcggtatgc
    39121 cgtccgaacg tttcaacaac aaacaccgat tatcgggaaa gaattgttca tgtcttcaaa
    39181 taaagcttca ttttttacac gtctgcgccg cttgtgccgc ttgacggtct ggctattcaa
    39241 aaccggcaaa aacctgcgcg gtattgacgg cggctgcccc gagtcgcgca atcgggcggt
    39301 aatcgagttg ggcagggggg ctctgacggc tttggatatc ggattggagg tgggcaggcc
    39361 cgcacccgaa catccgaacg gtgtcttggt tgccgccaac cacgtgtcct ggctggatat
    39421 tttcgcgatg agcgcggttt atccgagcag ctttatcgcc aagcaggaaa tcaaaagctg
    39481 gccggtattg ggcaagatgg ggcagaacgc gggaacggtg ttcatcaacc gcaattcgcg
    39541 gcgcgacatc gaaccgatta accgcgccgt ctgcgaaacc ttgcaacgcg gtcaaaacgt
    39601 cagttttttc cccgaagcgc ggacttcctc cggactgggg ctcctgccgt tcaaagccgc
    39661 gctgttccaa tccgccattg atgcgggggc aaaggttttg gcggtcgcgc tgcgttatta
    39721 tgacgaaacg ggaaaaagga cggctcgtcc ctcatatgcc gatgtcggtt tgccgacctg
    39781 cctgtggcgc attgtgtcta tgaaaaaatt aacgataaaa gtcgatttcg tttgcgtggc
    39841 ggatgcggcg gaaagcgaag accgttatgc tttaaaagat aaaatcgaag aaagcatccg
    39901 tgccgttgtc gccgacgatg cggatatcgc tgtctgaaac cggttgccgg aatgtggcag
    39961 tatgattcgc ttttgcggat
//
LOCUS       DAWFIE010000008        38713 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWFIE010000008 DAWFIE010000000
VERSION     DAWFIE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38713
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.008"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            233..511
                     /locus_tag="WGQ71_000379"
     CDS             233..511
                     /locus_tag="WGQ71_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706449.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKLQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMFLFIPIVLLVVYLFHYFGAF"
     gene            530..1678
                     /gene="earP"
                     /locus_tag="WGQ71_000380"
     CDS             530..1678
                     /gene="earP"
                     /locus_tag="WGQ71_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ0706450.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSLRLARVLHRELGWRVHLWTD
                     DVSALRALCPDLPDVPCVHQDIDVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSGALRKRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYSPETASVHRRLSDDLNGGEALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            1721..2281
                     /gene="efp"
                     /locus_tag="WGQ71_000381"
     CDS             1721..2281
                     /gene="efp"
                     /locus_tag="WGQ71_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ0706451.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(2330..3175)
                     /locus_tag="WGQ71_000382"
     CDS             complement(2330..3175)
                     /locus_tag="WGQ71_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ0706452.1"
                     /translation="MKNKTSSLLLWLAAIMLTACSPNKEDKTKETGASAASSPASAAS
                     SSAPQTDLQPAASAPDNVKQAESAPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPDSGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLTQGSEETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(3292..3873)
                     /gene="metW"
                     /locus_tag="WGQ71_000383"
     CDS             complement(3292..3873)
                     /gene="metW"
                     /locus_tag="WGQ71_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ0706453.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            complement(3870..5009)
                     /locus_tag="WGQ71_000384"
     CDS             complement(3870..5009)
                     /locus_tag="WGQ71_000384"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ0706454.1"
                     /translation="MSQNASVGIVTPQKIPFEVPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGKHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPLVTVKDWVKSQAALADYLSIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGDSLTRALQNVKAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKGCQQ"
     gene            5169..5369
                     /locus_tag="WGQ71_000385"
     CDS             5169..5369
                     /locus_tag="WGQ71_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706455.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALCEVAKISAWVLLG
                     SAAATLFWYLVMLYFKHIPDSY"
     gene            complement(5630..5755)
                     /gene="ykgO"
                     /locus_tag="WGQ71_000386"
     CDS             complement(5630..5755)
                     /gene="ykgO"
                     /locus_tag="WGQ71_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ0706456.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRKGKVYVICKSNPRFKARQR"
     gene            complement(5755..6030)
                     /locus_tag="WGQ71_000387"
     CDS             complement(5755..6030)
                     /locus_tag="WGQ71_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ0706457.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            6231..7109
                     /gene="metF"
                     /locus_tag="WGQ71_000388"
     CDS             6231..7109
                     /gene="metF"
                     /locus_tag="WGQ71_000388"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ0706458.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            7245..9521
                     /gene="metE"
                     /locus_tag="WGQ71_000389"
     CDS             7245..9521
                     /gene="metE"
                     /locus_tag="WGQ71_000389"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ0706459.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAAGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALTVDLPKEWV
                     EAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGIIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARSAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            9798..10535
                     /locus_tag="WGQ71_000390"
     CDS             9798..10535
                     /locus_tag="WGQ71_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ0706460.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTTPQVFIGGKYIGGSEDLEAYLAKN"
     gene            10789..12192
                     /locus_tag="WGQ71_000391"
     CDS             10789..12192
                     /locus_tag="WGQ71_000391"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0706461.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSNNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EANAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            12471..12848
                     /gene="sdhC"
                     /locus_tag="WGQ71_000392"
     CDS             12471..12848
                     /gene="sdhC"
                     /locus_tag="WGQ71_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ0706462.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            12842..13183
                     /gene="sdhD"
                     /locus_tag="WGQ71_000393"
     CDS             12842..13183
                     /gene="sdhD"
                     /locus_tag="WGQ71_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ0706463.1"
                     /translation="MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSVKVIWG"
     gene            13186..14949
                     /gene="sdhA"
                     /locus_tag="WGQ71_000394"
     CDS             13186..14949
                     /gene="sdhA"
                     /locus_tag="WGQ71_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ0706464.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRVNTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADSERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYQGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDINTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            15069..15776
                     /locus_tag="WGQ71_000395"
     CDS             15069..15776
                     /locus_tag="WGQ71_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ0706465.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            15780..16028
                     /locus_tag="WGQ71_000396"
     CDS             15780..16028
                     /locus_tag="WGQ71_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ0706466.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            16140..17423
                     /gene="gltA"
                     /locus_tag="WGQ71_000397"
     CDS             16140..17423
                     /gene="gltA"
                     /locus_tag="WGQ71_000397"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0706467.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            17628..20456
                     /locus_tag="WGQ71_000398"
     CDS             17628..20456
                     /locus_tag="WGQ71_000398"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ0706468.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDTVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            20556..21767
                     /gene="odhB"
                     /locus_tag="WGQ71_000399"
     CDS             20556..21767
                     /gene="odhB"
                     /locus_tag="WGQ71_000399"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ0706469.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAATAAAEAPAAAPA
                     EAAPAAAPAAAQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAA
                     AAPAVALPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKY
                     KEKFEKEHGVKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRG
                     LVVPILRDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTP
                     IINPPQSAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDA
                     LEDPARLLLDL"
     gene            complement(21819..21935)
                     /locus_tag="WGQ71_000400"
                     /pseudo
     CDS             complement(21819..21935)
                     /locus_tag="WGQ71_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            22132..23565
                     /gene="lpdA"
                     /locus_tag="WGQ71_000401"
     CDS             22132..23565
                     /gene="lpdA"
                     /locus_tag="WGQ71_000401"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0706470.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGSEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            23660..23947
                     /locus_tag="WGQ71_000402"
     CDS             23660..23947
                     /locus_tag="WGQ71_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706471.1"
                     /translation="MNKEIVGVFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAIMYLLARMWLPFSK"
     gene            24019..25185
                     /gene="sucC"
                     /locus_tag="WGQ71_000403"
     CDS             24019..25185
                     /gene="sucC"
                     /locus_tag="WGQ71_000403"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ0706472.1"
                     /translation="MNLHEYQAKELLAGYGLPVQGGILARNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVDNDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            25196..26086
                     /gene="sucD"
                     /locus_tag="WGQ71_000404"
     CDS             25196..26086
                     /gene="sucD"
                     /locus_tag="WGQ71_000404"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ0706473.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(26284..26661)
                     /locus_tag="WGQ71_000405"
                     /pseudo
     CDS             complement(26284..26661)
                     /locus_tag="WGQ71_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26633..26776)
                     /locus_tag="WGQ71_000406"
     CDS             complement(26633..26776)
                     /locus_tag="WGQ71_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706474.1"
                     /translation="MARYRRNFIAGGTFFFTVKLADPKSRLLVEHIDFAGSLYGCAKT
                     ISL"
     gene            26909..27175
                     /locus_tag="WGQ71_000407"
     CDS             26909..27175
                     /locus_tag="WGQ71_000407"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706475.1"
                     /translation="MKRQTVNQATACVRTAHTLHVDFEVPCNSQVGCVAQPRTRCWVC
                     RLRLAAAETPEEKAKDAAEEAKEEAKDAAEDAKEAAEDAKEEAK"
     gene            complement(27402..28343)
                     /locus_tag="WGQ71_000408"
     CDS             complement(27402..28343)
                     /locus_tag="WGQ71_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HEZ0706476.1"
                     /translation="MEYNAIMKTIAPSHIKPLMPELNQSQRNRIIFIDFYLCFLGKIN
                     RKDLMQAFQISPAVATRDFALYCSSFSKNLRFDTKSKFYLSTEDFEPQFPHSFENAIK
                     ILNGSLIFDIDSQHQPLLPFDLPPVLINPDVLIFSKISKALSLKKPLKISYHSISSGL
                     SEKVIVPLALVNDGFRWHLRAFDRTKNRFADFVLTRIIQANILLAENIEENETINNDI
                     DWNRIVKIELVPHPNHDEAKKVIEMDFGMTNGKLELALRASNIGYVLQQWRVDCSSDH
                     HIDDPAFRLWLLDNLVLYGINNAEHLAPAFDKKRSKP"
     gene            28481..28750
                     /locus_tag="WGQ71_000409"
     CDS             28481..28750
                     /locus_tag="WGQ71_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706477.1"
                     /translation="MKIKFAHLRHPAVGGGYINFAVFDAKSTNGNHSVVLKELTTEAR
                     RNGLKIDQSALIFEQHGRTQFYGDKNLVDFLSANWYPQWTHTLTI"
     gene            28754..28951
                     /locus_tag="WGQ71_000410"
     CDS             28754..28951
                     /locus_tag="WGQ71_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706478.1"
                     /translation="MEMIMTNKIKPGQNTGNNGGIYQEIGSRGGRKDNYATIKDNQKA
                     PPTQNPGSSWIQIKRTPDSSR"
     gene            28966..29136
                     /locus_tag="WGQ71_000411"
     CDS             28966..29136
                     /locus_tag="WGQ71_000411"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706479.1"
                     /translation="MFLGIFIALSGILTLLEMFNVISSDMKWGMPLMFTCLGFSIIFD
                     SFKAKQNRKQKE"
     gene            complement(29168..32014)
                     /gene="uvrA"
                     /locus_tag="WGQ71_000412"
     CDS             complement(29168..32014)
                     /gene="uvrA"
                     /locus_tag="WGQ71_000412"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ0706480.1"
                     /translation="MCNHHPKHSHGNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIASGSPEEVAKVKGSYTGKYLKNIL"
     gene            32169..32966
                     /locus_tag="WGQ71_000413"
     CDS             32169..32966
                     /locus_tag="WGQ71_000413"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ0706481.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVNGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAVAAKIQAAVQDVLKD"
     gene            complement(33944..36220)
                     /locus_tag="WGQ71_000414"
     CDS             complement(33944..36220)
                     /locus_tag="WGQ71_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0706482.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVGLETVTVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSKTGSIGLSWVGEKGFIGAAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDVDYDNPGLSCGFHDDDNAHAHTHNGKPWIDLRNKR
                     YELRAEWKQPLPGFEALRVHLNRNDYHHDEKAGDAVENFFNNKTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSAISEAVKQPMLLDNKVQHYSFFGVEQAEWDNFTLEGGVRV
                     EKQKASIQYDKALIDRENYYKQPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGVHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            36642..37232
                     /locus_tag="WGQ71_000415"
     CDS             36642..37232
                     /locus_tag="WGQ71_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ0706483.1"
                     /translation="MLLFIDNYDSFTYNIVQYFTELGQEVAVRRNDDITLEEIEALKP
                     QYLVIGPGPCSPKEAGISVEAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIERGTLPECLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLVEFQNFKPQKI"
     gene            37296..38354
                     /gene="trpD"
                     /locus_tag="WGQ71_000416"
     CDS             37296..38354
                     /gene="trpD"
                     /locus_tag="WGQ71_000416"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ0706484.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(38506..>38713)
                     /locus_tag="WGQ71_000417"
     CDS             complement(38506..>38713)
                     /locus_tag="WGQ71_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0706485.1"
                     /translation="TVVVPNAQSATLLPIIRKKVKPDGIVYTDTFRSYDVLDISEFSH
                     LRKFNGIPKEHLGLHLKKCQWHFK"
BASE COUNT         9633 a         9377 c        10026 g         9677 t
ORIGIN      
        1 ttcaaaccga cgcgccgggt tttcaatatg cccgtgcctg ctgccgcctt gtcctcaggc
       61 atcggcggca gcgtccgatt tttcggggaa cgcccgtctc gggcgtattt aaaggttcgg
      121 cggtgcggtg ttttcctgcg gcaaggcttc agacggcatc tccggtgcgt ccgttagaca
      181 aggcgtgcgc ttggggcgat aatggcgttt tgcttttttt gaaagcctta caatgtctcg
      241 aaacctgctt gtccgttggc ttgccgtctg cctcatcccg ttggcgacgc ttgccgtttt
      301 cgccgccaat ccgcccgaag acaaactcca gcatctgatc aacggcatca tccttgcctg
      361 cgaagcgacg tttttgttca aattcgtcct tttcgacacc atcaagcatc atttgaagca
      421 agagtttgat ttgaaacgtc aaactatgtt tctgtttatt ccgattgttt tgctggttgt
      481 gtatctgttc cactatttcg gcgcgtttta gcccgtttcc gttattccta tgaatactcc
      541 tccttttgtc tgctggattt tttgcaaggt catcgacaat ttcggcgata tcggcgtttc
      601 cctgcggctt gcccgtgttt tgcatcgcga actcggttgg cgggtgcatt tgtggacgga
      661 cgatgtgtcc gccttgcgtg cgctttgccc tgatttgccc gatgttccct gtgttcatca
      721 ggatattgat gtccgcactt ggcattccga tgcggcagac attgataccg cgcctgttcc
      781 cgatgtcgtc atcgaaactt ttgcctgcga cctgcccgaa aatgtgctgc acattatccg
      841 ccgacacaag ccgctttggc tgaattggga atatttgagc gcggaggaaa gcaatgaaag
      901 gctgcacctg atgccttcgc cgcaggaagg tgttcaaaaa tatttttggt ttatgggctt
      961 cagcgaaaaa agcggcggac tgatacgcga acgcgattac tgcgaagccg tccgtttcga
     1021 tagcggagcc ttgcgcaaga ggctgatgct tcccgaaaaa aacgcccccg aatggctgct
     1081 tttcggctat cggagcgatg tttgggcaaa gtggctggaa atgtggcgac aggcaggcag
     1141 cccgatgaca ctgttgctgg cggggacgca aatcatcgac agcctcaaac aaagcggcgt
     1201 tattccgcaa gatgccctgc aaaacgacgg cgatgttttt cagacggcat ccgtccgcct
     1261 cgtcaaaatc cctttcgtgc cgcaacagga cttcgacaaa ctgctgcacc tcgccgactg
     1321 cgccgtgata cgcggcgaag acagtttcgt gcgcgcccag cttgccggca aacccttctt
     1381 ttggcacatc tacccgcaag acgagaatgt ccatctcgat aaactccacg ccttttggga
     1441 taaggcgcat ggattttact cgcccgaaac cgcatcggta caccgccgcc tttcagacga
     1501 cctcaacggc ggagaggctt tatccgccac gcaacgcctc gaatgttggc aaaccctgca
     1561 acaacatcaa aacggctggc ggcaaggcgc ggaggattgg agccgttatc ttttcgggca
     1621 gccttccgca tccgaaaaac tcgccgcctt tgtttcaaag catcaaaaaa tacgctagaa
     1681 tagcgcgttt tacgacaacc gatttgattg gaaaatcaca atgaaaacag cacaagaact
     1741 gcgcgccggc aatgtattta tggtcggcaa cgatcctatg gtcgttcaaa aaaccgaata
     1801 catcaaaggc ggccgctctt ccgccaaagt cagcatgaaa ctgaaaaacc tgctgaccgg
     1861 cgcggcttcc gaaaccattt acaaagccga cgacaaattc gacgtggtca tcctgtcccg
     1921 caaaaactgt acgtacagct attttgccga cccgatgtac gtctttatgg acgaagaatt
     1981 caaccaatac gaaatcgaag ccgacaacat cggcgacgcg ttgaaattca tcgttgacgg
     2041 tatggaagac caatgcgaag ttaccttcta cgaaggcaac cccatctccg tagaactgcc
     2101 taccatcatc gtacgcgaag tcgagtacac cgagcctgct gtcaaaggcg acacttccgg
     2161 caaagtgatg aaaaccgcgc gtctggtcgg cggcaccgaa atccaagtga tgtcttacat
     2221 cgaaaacggc gacaaagtcg aaatcgatac ccgcaccggc gaattccgca aacgcgcctg
     2281 atatcctact tgaaaaatgc cgtctgaaag gctttcagac ggcatttttt tatcttcgcc
     2341 ccaagtttgg attgaagtag atgttttttt cgtaaacgac aatacgcgtg attttgccat
     2401 tttcgtcaaa atggatgtct aaaaacggtt tgtcgggatt gcgttcacgg ttggacagcc
     2461 ggaagagtga ttggttttca agcatttctc cgtgtatttt cagatagccg ggcaattggc
     2521 cgatgtattg gaaattgccg ccaagtccgt tgttgtgccg gctcgaatag gcaggggttg
     2581 ccgaaacttc aaaataacgc gtgcgtttcg gctcctcgcc ttgcccgcgg gtttcttcgc
     2641 tgccctgtgt cagcaacagc gtcgcttctc ggaggcggcg ttcctgttcc atcaaggcat
     2701 cttgtgcctg ccggctgatc gtgcgctttc cgtaacgcaa gtcttcaatt gccagtatga
     2761 ttttctgatg gtagtcggga tcttcagggc ggatgtcggg aaacagcagc cgctgtatgt
     2821 tgtcatagcc actgtcgggt aagccgaaac gggtttccag ttcgtgatgg gaaagggcaa
     2881 acagacagtc cgagtcgatg tgttcggcga tttgctgcat gagatcgtca atgccggtgg
     2941 cggggtgcag gtcggtgcaa tttgacggcg gcgcgctttc tgcctgcttg acgttatcag
     3001 gggcggatgc ggccggttgc aaatcggttt ggggcgcgga agacgaagcg gcggatgccg
     3061 gggacgaagc ggcagatgcg ccggtttctt tggttttatc ttctttgttc ggggaacacg
     3121 cggtcagcat gattgcggca agccataaga gaagtgatga ggttttgttt ttcattctat
     3181 tgtttccaat attaaaaagg ccgtctgaaa acctgccgtt tcatttttca gacggcctgt
     3241 tgttaataga accgaagaac ctgttattgc cgacaaggtt ctcaacctgt cttacccgac
     3301 gcggtaaaac gccaggctgc ccaaaaggtt ggggaaatgt ttgacctgtc tgttgcccgt
     3361 catcacggcg cgttcgagga cgcggatgtt gtttttggcg cacaacaaat caaagtcttt
     3421 gagcgtgcac caatggatat tgggcgtgtc gtaccaatgg tagggcatac gttcggaaac
     3481 cggcatatgt ccgccgagtg cgatttggac acggttgcgc cagtagccga aattcgggaa
     3541 gctgacaatc gcctgtttgg caacgcgcat caggcagcgc aggatttttt cggtattctg
     3601 catcgcttgg atggtttggc tcaacacaat cacatcaaaa ctttgatcgt tgaatgcggt
     3661 taaaccttct tccaaatcgg cttggataac attcacgccg cgcgacatcg cggcaatcac
     3721 gctgtcggtg tcgatttcga tgccgtagcc gctgcatttt ttgtgttcga ccaatgcggc
     3781 aagcagttcg ccgtcgccgc agcccaagtc caagacgcgg ctgccttcgg gtatccggtc
     3841 gtaaatcagt tgcaaatcat cgcgcaggtt cattgctgac atcccttata aacgttgttc
     3901 atataggcgg cgaccgcacg catataggct tcgtcttcca ttaaaaaggc atcgtgcccg
     3961 tgtgcggatt taacttcgat atactgcacg gatttttggg cggcaatcag tgccttgacc
     4021 agttcgtgcg aacgttcggg tgcgaaacgc cagtcggtgc tgaagctggc gacaaagaat
     4081 ttcgctttca cattttgcag ggcgcgggtc aggctgtcgc cgaaatctgc cgccggatcg
     4141 aaatagtcca aagccttggt catcagcaaa taggtgttgg catcaaaccg cccgacgaat
     4201 ttgtcgcctt gatagcgcag ataggattct acttcaaatt caacgccata gccgtattga
     4261 tagccgttgg aacgcaaatc gcgtccgaat tttttgccca aaccgtcttc ggcaagatag
     4321 gtgatgtgcc ccatcatgcg ggcaatccgc aagccccgag cgggaacggt gttgcggctg
     4381 cggtaatgtc cttcgttgaa atcgggatcg gtcaaaatcg cctgacgcgc cacatcgttg
     4441 aacgcgatat tttgcgtgga cagtttcggc gcggacgcaa tcaccaaggc gtggcgcacg
     4501 cgttcgggat aggaaatcgc ccactgcaaa gcctgcatgc cgcccaagct gccgccgaca
     4561 accgccgccc attgcccgat gctgagataa tcggcaagcg cggcttggga ttttacccag
     4621 tccttcaccg taaccagcgg aaaatccgcg ccgtattccc tacccgtttc aggattgatc
     4681 gacaaaggcc cgctgctgcc gtcgcagccg cccagattat tcaaaccgac cacgaaaaaa
     4741 cgttccgtat caatcggttt tccgggaccg accatattgt cccaccagcc cgtatattta
     4801 tcctccgccg aatgtttgcc cgcaacatga tggttgcccg acagcgcgtg gcagattaaa
     4861 accgcattgt ttttttcagc attcagctcg ccgtaggttt caatcatcag atcgaaacgc
     4921 ggcaaagttt taccgttttc caaaaccagt ggcacctcaa acggaatttt ttggggcgtt
     4981 acaatgccca ccgaggcatt ttgactcata tcctgttcca acaaatgcgg cgaaaagcgt
     5041 tattatatcg caaacggcat gattttttga cacggtcgga caagcagcct gacacgccga
     5101 aacattcgtt cccatgccgc cgcaaccgaa ccatactttt tcagacggtc ctaacccagc
     5161 ttcccgacat gataggcaga cttttccgta tttttttctt tttcgcactt gccgcgttga
     5221 ttatcaaccg ccttttcagc cgcaggcaaa aacgcgccct gtgcgaagtc gccaaaatca
     5281 gcgcatgggt actgcttggc tcagccgccg cgacactgtt ttggtatttg gtcatgctgt
     5341 atttcaaaca cattccggat tcgtattgac ggaaaaaatg ccgcctgaaa cgcatttttc
     5401 tgtttcagac ggcatatttg atgaaaaggg cttgcggtag gaggtgcttt atagtggatt
     5461 aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc
     5521 tgaagcacca agtgaatcgg ttccgtacta tttgtactgt ctgcggcttc gtcgccttgt
     5581 cctgattttt gttaatccac tatacaaccg aagcaggaag ggcagggggt cagcgttggc
     5641 gcgctttaaa acgcggattg cttttgcaga tgacgtaaac tttgccttta cgtctgacaa
     5701 tctgacagtc gcggtggcgt tgtttggcgg ttttgagtga agacagaacc tgcattattt
     5761 gtcctttcta aacgatgaca tgacggattg gaaacgttgg ttgaatttgc tggcacggcc
     5821 ttcggtgttg acgttgcgct gtttgccagt atagacggga tgggatgcgg aagaagtatc
     5881 cagcgaaaac agcggatatt ctttgccgtc tgtccaaacc atcgtttttc cgtgtgtttc
     5941 ggcacaggag cggattaacc agccttcatt ggcgctgctg tcgaaaaaaa ggacggttcg
     6001 ataattgtca ggatgaatat tcggtttcat atatcgcctt agttgaattg ttataacata
     6061 acaaacttta tcataaatta gaaggcttgt acaaggaaat ttaactattt ttgtaatata
     6121 ttagaaattt tcatgataaa tctgaaaatt ttgaaattga ctcatgtttg gcgtaacttt
     6181 attatgttgc ctgaaaatat catataaaag ataataaaag gtacgcagcc atgaattatg
     6241 caaaagaaat caatgcgtta aataacagtc tttctgattt gaaaggcgac attaacgttt
     6301 cgtttgaatt tttcccgccg aaaaacgaac aaatggaaac gatgctgtgg gactccatcc
     6361 accgtctgca aaccctgcat cccaagttcg tatccgtaac ttacggcgca aattccggcg
     6421 aacgcgaccg cacgcacggc atcgtcaaac gcatcaaaca ggaaaccggc ttggaagccg
     6481 cgcctcacct gaccggtatc gacgcttctc ccgacgaatt gcgccaaatt gccaaagact
     6541 attgggacag cggcatccgc cgcattgtcg ccctgcgcgg cgacgagccg cccggttatg
     6601 agaaaaaacc gttttacgcc gaagacttgg tcaaactgtt gcgctccgtc gccgacttcg
     6661 acatttctgt agcggcatat cccgaagtgc atcccgaagc gaaatccgca caagccgacc
     6721 tgattaattt gaaacgcaaa atcgatgcgg gcgcgaacca cgtcatcacc caattcttct
     6781 tcgatgtgga acgctacctg cgcttccgcg accgctgcgt gatgttgggc atcgatgtgg
     6841 aaatcgtccc cggcatcctg cccgttacca acttcaagca gctcggcaaa atggcgcagg
     6901 taaccaacgt caaaatccca agctggctgt cgcaaatgta tgaaggtttg gacgacgacc
     6961 aaggcacgcg caatctggtg gcggcaagta tcgccatcga tatggtcaaa gtcctgtccc
     7021 gcgaaggcgt gaaagatttc cacttctata cgcttaaccg cagcgagctg acttacgcca
     7081 tttgccatat tttaggcgtg cgcccttaaa gccgaaaacg gcttcagacg gcatacgagg
     7141 ccgtctgaaa agcaaaacgc cgccccatcc gagccattct gatttacaat accgaccgat
     7201 tcggatcgaa ccggtcctta caaaatccaa ctggagagtt caacatgaca acattacatt
     7261 tctcaggctt cccgcgtgtc ggcgccttcc gcgaattgaa attcgcacaa gaaaaatact
     7321 ggcgcaaaga aatcagcgag caagaattgc tggctgttgc taaagacttg cgcgagaaaa
     7381 actggaaaca ccaggccgct gccaacgccg attacgttgc cgcaggcgat ttcactttct
     7441 acgaccacat cctcgacctg caagtcgcca ccggcgcgat tcccgcccgc ttcggcttcg
     7501 acagccaaaa cctgtctttg gaacaattct tccaactggc gcgcggtaac aaagaccaat
     7561 tcgctatcga aatgaccaaa tggttcgaca ccaactacca ctacttggtg cctgaattcc
     7621 acgccgatac cgaattcaaa gccaatgcca aacactatgt tcaacaactg caagaagccc
     7681 aagccctcgg tctgaaagcc aaaccgaccg ttgtaggtcc gttgactttc ctgtgggtgg
     7741 gtaaagaaaa aggcgccgtc gaattcgacc gtctgagcct gttgcctaaa ctgttgcctg
     7801 tttacgttga aatcctgact gctttggttg aagccggtgc cgagtggatt caaatcgacg
     7861 agcctgcttt gactgtcgat ctgcctaaag aatgggtaga agcctacaaa gacgtttacg
     7921 ctactttgag caaagtaagt gccaaaatcc tgctgagcac ttacttcggt tctgttgccg
     7981 aacacgccgc attgttgaaa tccctgcctg ttgacggcct gcacatcgac ttggtacgcg
     8041 ctcccgagca actggacgca tttgccgact acgacaaagt tctgtctgca ggcatcatcg
     8101 acggccgcaa catctggcgc gccaacctga acaaagtttt ggaaactgtt gagcctctgc
     8161 aagccaaact gggcgaccgt ttgtggattt ccagctcttg ctctctgctg cacactccat
     8221 ttgacttgtc agttgaagaa aaactgaaag ccaacaaacc cgacctgtac tcttggttgg
     8281 cattcaccct gcaaaaaacc caagaattgc gcgttctgaa agctgcattg aacgaaggcc
     8341 gtgattctgt tgccgaagaa ctcgccgcca gccaagctgc tgccgactcc cgtgccaaca
     8401 gcagcgaaat ccatcgtgca gacgttgcca aacgcctggc cgatttgcct gccaacgcag
     8461 accaacgcaa atctccattt gccgaccgta tcaaagcgca acaagcatgg ttgaacctac
     8521 ctctgctgcc gacgaccaac atcggttctt tcccgcaaac caccgaaatc cgccaggcac
     8581 gctcagcctt caaaaaaggc gaactgtctg ccgccgatta cgaagccgcg atgaaaaaag
     8641 aaatcgcctt ggtggttgaa gagcaagaaa aactggactt ggacgtactg gtacacggcg
     8701 aagccgagcg taacgacatg gttgaatact tcggcgaatt gttgagcggt tttgcattca
     8761 ctcaatacgg ctgggtacaa agctacggct cacgctgcgt gaaaccaccg attatctttg
     8821 gcgacgtaag ccgtcctgaa gccatgaccg tggcttggtc tacttacgca caaagcctga
     8881 ccaaacgccc gatgaaaggt atgttgaccg gccctgtaac cattctgcaa tggtctttcg
     8941 tccgcaacga cattcctcgc tctaccgtgt gcaaacaaat cgcactggct ctgaacgacg
     9001 aagtattgga tctggaaaaa gccggcatca aagtcatcca aattgacgaa cctgccatcc
     9061 gcgaaggctt gccgctgaaa cgcgccgatt gggatgccta cctgaactgg gcgggcgaat
     9121 ccttccgcct gtcctctgcc ggttgcgaag acagcaccca aatccacact catatgtgtt
     9181 actccgagtt caacgacatc ctacctgcca tcgccgctat ggatgctgat gtcatcacca
     9241 tcgaaacttc acgttccgat atggaactct tgaccgcgtt cggcgaattc aaatacccga
     9301 acgacatcgg cccgggcgtt tacgacatcc acagcccgcg cgtaccgact gaagctgaag
     9361 ttgagcatct gttgcgcaaa gccatcgagg ttgtaccggt tgaacgtctg tgggttaacc
     9421 cagactgcgg cctgaaaacc cgtggctgga aagaaactct ggaacaactc caagtgatga
     9481 tgaacgtaac ccacaaactg cgtgccgaac tggcgaaata agcagggata acgtgatcgg
     9541 atgtcgtctg aatacttttc agacggcatt tctgttttca aatgggcgta ggggagtttc
     9601 taagaatttt tctcaaataa gacttgtttt ttttaggcat ggctttatat actgtaaaaa
     9661 gttggtcgag gttggtgatt aatagttttg agtaattaat attatttgaa ctataaatta
     9721 tacaaatcga cttattcggg gtagaatgcc cagcgattca caattatttc tcaaaccaat
     9781 ctattaagga gcttaaaatg gctttgcaag atcgtaccgg tcaaaaagta ccttccgtag
     9841 tattccgcac ccgcgtcggc gacacttgga aagatgtgtc taccgatgat ttgttcaaag
     9901 gcaaaaaagt agtcgtattc tccctgcccg gtgcatttac cccgacttgt tcttcttcac
     9961 acctgccgcg ttacaacgaa ttgttcggcg cgttcaaaga aaacggcgtt gacgcaatct
    10021 actgcgtatc tgtaaacgat acgttcgtaa tgaacgcttg ggctgccgaa gaagaatccg
    10081 acaacatcta catgattcct gacggcaacg gcgaatttac cgaaggtatg ggtatgctgg
    10141 tcggtaaaga agacttgggc ttcggtaaac gctcttggcg ttactccatg ctggttaacg
    10201 acggcgtggt tgaaaaaatg ttcatcgaac ctgaagaacc gggcgatccg ttcaaagtat
    10261 ccgatgcaga tactatgctg aaattcgttg ctcccgattg gaaagctcaa gagtctgttg
    10321 ccatctttac caaacccggc tgccaattct gtgctaaagc caaacaagct ttgcaagaca
    10381 aaggtttgtc ttacgaagaa atcgtattgg gcaaagatgc aaccgttact tccgtacgcg
    10441 ccattaccgg caagatgact acccctcaag tcttcatcgg cggcaaatac atcggcggca
    10501 gcgaagattt ggaagcctac ttggctaaaa actaattgcc tagtgcttaa gacggtttga
    10561 ataaaaccgt ctgaacagga aaatagagtt attcagacga tacgctacac ggtcgttctg
    10621 aatagctcta tatttacaag tagaatttga gattattgtt ttaaatcgaa acttttaaaa
    10681 tggctattgg ctttatattg tcgatagttt attaaaaaag ccgtctgaaa acctgatacg
    10741 catcacgctg atgctgcttc ggacgcttaa taaagaagga caaagattat gaaaaaaatt
    10801 caagcagatg tcgtcgtaat cggcggcggt actgccggta tgggcgcgtt tcgcaatgcc
    10861 cgtttgcatt cgaacaatgt ttacctgatt gaaaacaatg tgttcggcac gacctgcgcg
    10921 cgggtgggtt gtatgccttc caaactcttg attgccgccg cagaagcgcg ccaccacgca
    10981 ctgcataccg acccgttcgg cgtgcatttg gacaaagaca gcatcgtcgt caacggtgaa
    11041 gaggtcatgc agcgcgttaa atccgagcgt gaccgttttg tcggctttgt cgttgccgat
    11101 gtggaagagt ggcctgccga caagcgcatt atgggttcgg ctaaatttat cgacgagcat
    11161 accgtccaaa tcgacgagca tactcaaatt acggcaaaaa gtttcgtgat tgctaccggt
    11221 tcgcgtcccg tcatcctgcc gcaatggcag tctttgggca atcgtttgat tatcaacgat
    11281 gacgttttct catgggatac gctgcctaag cgcgttgccg tgttcgggcc gggtgttatc
    11341 ggtttggaac tgggtcaggc attgcaccgt ttgggcgtga aagttgaaat tttcggtttg
    11401 ggcggaatca tcggcggcat ttccgacccc gtcgtttcag acgaggcgaa cgccgtgttc
    11461 ggcgaagaat tgaaactgca tctggatgct aaaaccgagg tcaaactcga tgcagacggc
    11521 aatgtagaag tccattggga gcaggatggc gaaaaaggcg tatttgttgc cgaatatatg
    11581 ctggcagccg tgggccgccg tccgaacgtt gacaatatcg gtttggaaaa tatcaatatc
    11641 gaaaaagatg cgcgcggcgt acctgttgcc gacccgctga ccatgcagac cagtattccg
    11701 catatcttca tcgcaggcga tgcgtccaac caactgcctc tgctgcatga agctgccgac
    11761 caaggcaaga ttgccggcga taacgcgggc cgctacccga atatcggcgg cggtttgcgg
    11821 cgcagcacca tcggcgtggt gtttaccagt ccgcaaatcg gctttgtcgg tctgaaatac
    11881 gcgcaggttg ccgcgcaata ccaagccgac gaatttgtca tcggcgaagt atcgttcaaa
    11941 aaccaaggcc gcagccgcgt gatgctggtg aacaaaggcc atatgcgcct gtatgccgaa
    12001 aaagccaccg gccgctttat cggcgcggaa atcgtaggcc ctgccgccga acatttggcg
    12061 cacctgttgg cttgggcaca tcaaatgaag atgaccgttc cgcaaatgct ggatatgccg
    12121 ttctaccatc ccgttatcga ggaaggtctg cgtaccgcgt tgcgcgatgc cgatgcgaaa
    12181 ttgaaagcct gaccgatatg gcaaaacaat gccgtctgaa attttttcag acggcatttt
    12241 gtttttgggg atggggtcgg atgctgatac cgtgtcggga agggggcggc aaaactaaaa
    12301 atctttcttt taatctgctg tttccacgcg tgtttgtcaa aatctatcag tttgttttta
    12361 aaatacactg ttcaaaatgg gataaaacag gtaaattaac gtttatgtaa cccagtgtag
    12421 caatgggttt acggtttttg agtcgatata taactacaga ggaattgact atgtctgcca
    12481 aaccgcgtcc tgtttatctg gatttgccga acatccgtct gccgataccc gggatagttt
    12541 ccatccttca ccgcatcagc ggggtcgggc tgtttattat gctgcctttc ctgctgtatt
    12601 tcctgtccgg taccctgagt caagagtctg catttgaaac ttaccgtgcc attgtttccc
    12661 atcctttggt caagctggtt ttaatcggtg tattgtgggc ttatctgcac cattctctcg
    12721 ccggtatccg ctttttattt ttggatgcgc acaaaggcct tgagctgaat actgcgcgca
    12781 ataccgctaa agccgtattt gcttctgcat tggttttgac tgtcgttttg ggagcgttgt
    12841 tatggtagaa cgtaaattga ccggtgccca ttacggtttg cgcgattggg tgatgcaacg
    12901 tgcgactgcg gttattatgt tgatttatac cgttgcactt ttagtggttc tattttccct
    12961 gcctaaagaa tattcggcat ggcaggcatt ttttagtcaa acttgggtaa aagtatttac
    13021 ccaagtgagc ttcatcgccg tattcttgca cgcttgggtg ggtatccgcg atttgtggat
    13081 ggactatatc aaacccttcg gcgtgcgttt gtttttgcag gttgccacca tcgtttggct
    13141 ggtcggctgt ctcgtgtatt cagttaaagt gatttggggg taagtatggg ttttcctgtt
    13201 cgcaagtttg atgccgtgat tgtcggcggt ggtggtgcag gtttacgcgc agccctccaa
    13261 ttatccaaat ccggtctgaa ttgtgccgtt ttgtctaaag tgttcccgac ccgttcgcat
    13321 accgtagcgg cgcagggcgg tatttccgcc tctctgggta atgtgcagga agaccgttgg
    13381 gactggcaca tgtacgatac cgtgaaaggt tccgactggt tgggcgacca agatgcgatt
    13441 gagtttatgt gtcgcgctgc gcctgaagcc gtaattgagt tggaacacat gggtatgcct
    13501 tttgaccgcg ttgaaagcgg caaaatttat cagcgtcctt tcggcggtca tactgccgaa
    13561 cacggtaaac gcgcggtaga acgtgcttgc gcggttgccg accgtaccgg tcatgcgatg
    13621 ttgcatacgt tgtatcaaca aaacgtccgt gtcaatacgc aattctttgt ggaatggacg
    13681 gcacaagatt tgattcgtga tgaaaacggc gatgtcgtcg gcgtaaccgc catggaaatg
    13741 gaaaccggcg aagtttatat tttccacgct aaagctgtga tgtttgctac cggcggcggc
    13801 ggccgtattt atgcgtcttc taccaatgcc tatatgaata ccggcgatgg tttgggtatt
    13861 tgcgcgcgtg caggtatccc gttggaagac atggaattct ggcaattcca cccgaccggc
    13921 gtggcaggtg cgggcgtgtt gattaccgaa ggcgtacgcg gcgagggcgg tattctgttg
    13981 aatgccgaca gcgaacgctt tatggaacgc tatgcgccga ccgtaaaaga cttggcttct
    14041 cgcgacgttg tttcccgcgc gatggcgatg gaaatctacg aaggtcgcgg ctgcggtaaa
    14101 aacaaagacc atgtcttact gaaaatcgac catatcggcg cagaaaaaat tatggaaaaa
    14161 ctgccgggca tccgcgagat ttccattcag ttcgccggta tcgatccgat taaagacccg
    14221 attcctgttg tgccgactac ccactatatg atgggcggca ttccgaccaa ttaccaaggc
    14281 gaagtcgttg tgccgcaagg agacgaatac gaagtgcctg taaaaggcct gtatgccgca
    14341 ggtgagtgcg cctgtgcttc cgtacacggt gcgaaccgtt tgggtaccaa ctctctgctg
    14401 gacttggtgg tgttcggtaa agctgccggc gacagcatga ttaaattcat caaagagcaa
    14461 agcgactgga aacctttgcc tgcggatgcg ggcgagctga cccgccaacg tatcgagcgt
    14521 ttggacaatc aaactgatgg cgaaaacgtt gatgcattgc gccgcgaact gcaacgctct
    14581 gtacaactgc acgccggcgt gttccgtact gatgagattc tgagcaaagg cgttcgagaa
    14641 gtcatggcga ttgccgagcg tgtgaaacgt accgaaatca aagacaagag caaagtgtgg
    14701 aataccgcgc gtatcgaggc tttggaattg gataacctga ttgaagtggc gaaagcgact
    14761 ttggtgtctg ccgaagcacg taaagaatca cgcggtgcgc acgcttcaga cgaccatcct
    14821 gagcgcgatg atgaaaactg gatgaaacat acgctgtacc attcagatat caataccttg
    14881 tcctacaaac cggtgcacac caagcctttg agcgtggaat acatcaaacc ggccaagcgc
    14941 gtttattgat gcgttttcag acggtcttcg cctcaaaggt cgtctgaaat ctaaccatac
    15001 ccacattgaa ctgcttgaat ttataataca aaatcattgg gcagttgatg agaaaaggaa
    15061 cacttctcat ggaaaaaatg agttttgaaa tttaccgtta caacccggat gttgatgcca
    15121 agccttatat gcagcgttac gagttggaat tggaaccgac cgacgtgaaa cttttggatg
    15181 ctttggtacg cctgaaagca caagacgata ccttgtcttt ccgccgctcc tgccgcgaag
    15241 ggatttgcgg ttctgacggt atgaacatca acggcaaaaa cggcttggcg tgtttgaccg
    15301 atctgcgtgg cttgaaacag ccggttaaaa ttcgtcctct gccaggtctg cctgttatcc
    15361 gtgacctgat tgtggatatg acccagttct tcaaacaata ccattccgtc aaaccttatg
    15421 ttgtcaacga taatccgatt gatgcggaca aagagcgtct gcaaactcag gaagagcgta
    15481 aagagttgga cggtttgtac gagtgtattt tgtgcgcctg ctgttcgact gcctgcccgt
    15541 cattttggtg gaaccctgat aaattcgtcg gtccgtccgg tttgctgaat gcttaccgtt
    15601 tcattgcgga cagccgtgat accatcacta atgagcgttt ggataatctg aacgacccat
    15661 accgtttgtt ccgttgccac accattatga actgcgtaga cgtatgtcct aaacacttga
    15721 atccgacccg agccatcggt aagattaaag agattatgtt gaaacgggcc gtttaagaaa
    15781 tgatggtttt tgacgatatt gccaaacgga aaatccgttt tcaaacccgc cggggattgt
    15841 tggaattaga tttaatcttc ggcaggttta tggaaaaaga attcgagcat ttgagcgata
    15901 aagagctgtc cgagttttcc gaaatccttg aatttcaaga tcaagaattg cttgccttga
    15961 ttaacgggca ttcggaaacg gacaaagggc accttatccc gatgcttgaa aaaatcagac
    16021 gggcatgagg catctgaaat ctgcctgcag gcagatttca aaatgcaaaa gccgtctgaa
    16081 ggcaaagaac gtgctgcgga tgcagtaacg tgggttataa cttgcaaagg agcaataata
    16141 tgtccaaatc aatcaaactc aacgtaccgg gtcaggcagg tttggagctg ccggtattgg
    16201 aagccagcat cgggcacgat gtggttgaca ttcgggggct gacaaaaaat acaggtttgt
    16261 tttctttcga ccccggattt gtttcaaccg caagctgtga gtctaaaatt acttacatcg
    16321 acggcgatca aggcttgctt tattatcgcg gataccccat cgagcagctg gccgaaaagt
    16381 ccgattattt ggaagtctgc tacctgttga tttacggcga actgccgact cccgagcaaa
    16441 aggcagaatt tgacaatacc gtccgccgcc acacgatggt gcatgaacag ctgacttggt
    16501 tcttccgggg gttccgccgc gacgcgcatc cgatggcgat gatggtcggc gtggtcggtg
    16561 cgctgtctgc gttctaccaa gacagcttgg acattagcaa tcccgaacac cgcaaaatcg
    16621 cgatttaccg cctgatttcc aaaatcccga ccattgcggc aatgtgctac cgctattcaa
    16681 acggtctgcc gttcaattat ccgaagaata atctttctta ttccgaaaac ttccttcata
    16741 tgatgttcgc cacgccgtgt gaagactaca aacccaatcc cgttttggca cgcgcgctcg
    16801 accgcatctt tattttgcat gccgaccacg agcaaaacgc ctcaacttca accgtccgtc
    16861 tggcagggtc ttcgggtgcg aacccgtttg cctgtattgc tgccggtatc gcctgcctgt
    16921 ggggtgcttc gcacggcggt gcgaacgaag ccgtgttgaa aatgttggac gaaatcggcg
    16981 atgtgtctaa tgttgccgca tacatggaag gtgtgaaaca acgcaaatac cgtctgatgg
    17041 gcttcggtca ccgcgtgtac cgcaatatgg atccgcgtgc cagcattatg cgcgaaacct
    17101 gctatgaagt tttgaaggaa ttgggcttgg aagacagtcc gaaattcaaa ctggcgatgg
    17161 aattggaaca gattgcgctg aaagacccgt tctttatcga acgcaaactg tatccaaacg
    17221 tcgatttcta ttccggcatc gtcctgtccg cgctgggcat cccgaccgaa atgtttaccg
    17281 tcatcttcgc cctgtcgcgc agcgtgggct ggatttcgca ctggcacgag atgattagcg
    17341 atccttcgct gaaaatcggc cgcccgcgcc agctttatac cggttcggaa cgccgcgatt
    17401 atgtgccgcc aggcgagagg taacatacat taatatattg tcaaacaggc aatatcagag
    17461 aaccggattg tttcccgaat ccgtctgatt gtagtcggat gaaatcaaga caagcaatcc
    17521 ggtttaaaat agggtagaat aaaatgtctt ttcagacggc atcagtttag ccgtcaggac
    17581 gcggacttct accctttgtt tatattttaa agaaaagagc gcacgccatg atggacgaaa
    17641 aactcaattt ctcttacctg ttcggttcga acgcacccta cattgaggaa ttgtacgagg
    17701 cttttttgga aaaccccgat acggttgatg aaaaatggaa gcagtatttc accgatttga
    17761 gcaaacagcc ggggacggtt gctgtcgatg tcgcacacac accgattcgc gaatcatttg
    17821 ttactttggc gaaaaagaaa attgcatctg ccgttgcggg cggtgcggat gaggcaatgc
    17881 tgaaaaagca agtcagcgtt ttacggctga tttctgccta tcgtatccaa ggcgtgggtg
    17941 cagcccaact tgatccgctc aaacgtatcc ccccgcgcga tattgaagcc ctcgatccga
    18001 aattccacgg tctgtctgat gccgatatgg cgcttcaatt caatatgggc gagggcgatt
    18061 ttgccaatcg cggcaaactg cctttgtccc aaatcatcag caacctcaaa caaacctact
    18121 gcggccacat cgcattggaa tatatctata ttcccaatac cgaagagcgc cgctgggtac
    18181 gcaattattt tgaaagcgta ttgtccacac cgcattacaa tgccgatcaa aaacgccgta
    18241 tcttgaaaga gatgactgct gccgagactt tggaacgtta tctgcatacc aaatatgtcg
    18301 gtcagaaacg tttcggtgtc gaaggcggcg aaagcgcgat tgccggtttg aactacctga
    18361 ttcaaaacgc cggtaaagac ggcgtggaag aagtcatcat cggtatggcg caccgtggcc
    18421 gtctgaatgt tttggtgaac attttgggca aaaaacccgg cgatttgttt gccgaatttg
    18481 aaggtcgtgc cgaaatcaaa ctgcccagcg gcgacgtgaa ataccatatg ggcttcagct
    18541 ccgatattgc taccccgcac ggcccgatgc atgtttcttt ggcgttcaac ccgtcccact
    18601 tggaaatcgt taaccctgtt gttgaaggtt ctgcgcgtgc caaacaaaaa cgtttgggcg
    18661 aaaacggccg cgacaaagtc ttgccggtat tgattcacgg cgattccgca tttatcggtc
    18721 tgggtgtcaa ccaagcaaca ttcaacctgt ctaaaacacg cggttatacc accggcggta
    18781 cggttcatat cgtcatcaac aaccaaatcg gctttaccac ttccgatatc cgcgataccc
    18841 gttcaaccgt acactgtacc gatatcgcaa aaatggtttc cgccccggtt atccatgtga
    18901 acggcgatga tcctgagcgt gtttgctttg ctatccaagc cgctttggat taccgcaaaa
    18961 aattccataa agacatcgtg attgacgttg tctgctaccg taaatggggt cacaacgagg
    19021 gcgatgatcc gaccttgacc caaccgatga tgtacaaaaa agtatcgcaa caccccggtg
    19081 cgcgtgcttt gtacaccgag caactgattg ccgaaggcgt ggtaacccaa gccgaggcag
    19141 acggttacat ccaagcctac cgtgatgctt tggacaaagg cgagcacgtc gagcaaacaa
    19201 cgttgagcaa cttccaacgc acgcaaatcg actggagcaa ataccaaggc aaagattggc
    19261 gcgaacacat cgaaaccggt ttgcctgccg cagatattga acgtctcact gagaagttta
    19321 ccgccgtacc ggaaggcttt gccctgcatc cgactgcaaa acgtgtgatt gaagcgcgta
    19381 aagccatggc atccggcaaa caggccatag attggggtat ggccgaaacc ttggcatacg
    19441 ccagcctcgt aaccaaaggt cacggcgtgc gtatttccgg tgaggactcg ggacgcggta
    19501 cgttctcgca ccgccacgcc gtattgcacg atcaaaaacg cgaaaaatgg gacgacggta
    19561 cttatgttcc cctgcgccat atgggcgaag gaatgggcga gttcctggtt atcgattcca
    19621 tcttgaacga agaggccgtg atggcgttcg agtacggctt tgcctgctcc gctcctgaca
    19681 agctcaccat ttgggaagct caattcggtg acttcgccaa cggcgcgcaa gtgacgattg
    19741 accaattcct gtcttcaggc gaaaccaaat ggggtcgctt gtgcggtctg accaccatcc
    19801 tgccgcacgg ttacgacggt caaggccctg agcactcttc tgcacgcgta gaacgttggt
    19861 tgcaactgtg ttctgagaac aatatgcaag tcatcatgcc gtctgaagcg tcgcaaatgt
    19921 tccacctctt gcaacgtcaa gtcttaggtt cataccgcaa accgctagtg attttcatgt
    19981 ccaaacgcct gttgcgattc aaaggggcaa tgagcccgct ggaaaacttc accgaaggtt
    20041 cgactttccg tccggttatc ggcgataccg ccgaacgcgc aagcaacgac agcgtgaaac
    20101 gcgtggtatt gtgtgccggt caggtttact atgacttgga agccggtcga gccgaacgta
    20161 aactggaaga tgatgtcgct attgtccgcg ttgagcagct gtatccgttc ccatacgacg
    20221 aggttaaagc cgaactggcg aaatatccga acgcaaaatc tgtggtttgg gcacaagaag
    20281 agccgaaaaa ccaaggcgcg ttctaccaaa tccgccaccg catcgaagac gttatcagcg
    20341 aagagcaaaa actgtcttat gccggtcgtc caagcagcgc atcgcctgca gtgggctact
    20401 caagcaaaca cattgctcaa ttgaaacaat tggttgaaga cgctttggca ttgtaagcca
    20461 agtagcattc cgtctgaggc tgctcaggcg gaacacccat atgcagaatg aaaaacacac
    20521 aacaggccgt ctgaaagggc cattggagac acaaaatgat tattgatgta aaagtaccta
    20581 tgctgtctga aagcgtatct gaaggcacgc tcttggaatg gaagaaaaaa gttggcgaag
    20641 cagttgcccg tgacgaaatc ctgatcgata tcgaaacgga caaagtggtt ttggaagtac
    20701 cttctccaca agccggcgta ttggttgaaa tcgtagcgca agacggtgaa accgttgttg
    20761 ccgaccaagt tttggcacgt atcgataccg ctgctactgc cgctgctgaa gcacctgccg
    20821 ctgctactgc cgctgctgaa gcacctgccg ccgctcctgc cgaagctgcc ccggccgccg
    20881 ctcctgctgc tgcacaaaac aacgccgcta tgcctgccgc cgccaaactg gctgcagaga
    20941 gcggtgttga cgtgaacgca ttgcaaggtt ccggccgtga cggtcgcgta ttgaaagaag
    21001 acgtacaaaa tgccgctgcc aaacctgccg cagccgctgc tcctgctgtt gcacttcctg
    21061 ccggcgcacg tcctgaagaa cgcgtaccaa tgagccgcct gcgtgcccgt gttgcagaac
    21121 gcctcttggc ttctcaacaa gaaaacgcca ttctgactac attcaacgaa gtcaacatga
    21181 aaccaatcat ggacttgcgt gcgaagtaca aagaaaaatt cgagaaagaa cacggcgtga
    21241 aactgggctt tatgtccttc ttcgttaaag ccgctgttgc cgccctgaaa aaatacccgg
    21301 ttgtgaatgc ttctgttgac ggcaaagaca tcgtgtacca cggctacttc gacatcggta
    21361 tcgcaattgg cagcccacgc ggtttggttg tgccaatcct gcgcgatgcc gaccaaatga
    21421 gcattgccga catcgaacaa gcaattgttg attacgcgaa aaaagccaaa gacggcaaaa
    21481 tcgctatcga agatctgacc ggcggtacat tcagtattac caacggcggt actttcggtt
    21541 ctatgatgtc caccccgatc atcaatccgc ctcaatctgc gattttgggt atgcacgcca
    21601 ctaaagagcg cgctgtggtt gaaaacggcc aagttgttgt ccgtccaatg atgtatctgg
    21661 ctctgtctta cgaccaccgt atcattgacg gccgcgaagc tgtattgacc ttggtagcca
    21721 ttaaagacgc gttggaagac ccagcccgcc tgttgttgga tctgtaatcg tttcagacgg
    21781 ccttttattt gttaatgaaa aggccgtctg aatttttata gtggattaaa tttaaaccag
    21841 tacggcgttg cctcgccttg ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc
    21901 tgatttaaat ttaatccact atatttagat gtagcgtaat gtagtatcgt gctacaatag
    21961 gctcaacgaa cgattgaggc cgtctgaaac atttgattcg aatgaatcgg cagatatgga
    22021 cttttagacg gctttttctt aaaaccatca aaacgcagtc attcaaaata aaaaagaaac
    22081 gaaaagtatc gtttttattt tgagatactg ctaaaagcaa aggatgacac gatgtctcaa
    22141 tatgatgtag tagtgattgg tgcaggcccg ggcggatacg ttgccgccat ccgtgccgca
    22201 caactgggtt tcaaaactgc ctgtgtcgat gcaggcgtta acaaagcagg caatgcccct
    22261 gcattgggcg gtacttgctt gaacgtaggc tgtatccctt ctaaagccct gttgcaatcc
    22321 agcgaacatt tccacgctgc gcaacacgat tttgccgaac acggtatcac tgtcggcgac
    22381 gtaaaattcg acgcggccaa aatgattgag cgcaaagatg ccatcgtgac caaactgacc
    22441 ggcggcgtga aattcctgtt ccaaaaaaac aaagtaacca gcctgttcgg tactgcttcc
    22501 tttgccggta aaaatggcga tgcttaccaa atcgaagtcg ataacaaagg cgagaaaacc
    22561 gttatcgaag ccaaacacgt catcgtagcg accggttccg taccgcgtcc gctgccacaa
    22621 gtcgctatcg acaacgttaa cgtattggac aacgaaggcg cattgaacct gaccgaagta
    22681 cctgccaaac tcggcgtgat cggttccggc gtgattggtt tggaaatggg ttccgtatgg
    22741 aaccgcgtgg gttcagaagt taccattctt gaagccgcac cgactttctt ggctgccgcc
    22801 gaccaacaaa tcgccaaaga agccttcaaa tacttcacca aagagcaagg tctgagcatc
    22861 gaattgggcg tgaaaatcgg cgacatcaaa tctgaaggca aaggtgtttc cgttgcttac
    22921 gaaactgctg ccggcgaagc caaaaccgaa gtattcgaca aactgatcgt tgccatcggc
    22981 cgtattccaa acaccaaagg cctgaacgcg gaagccgtag gcttggaaaa agacgagcgc
    23041 ggctttatca aagtagatgg cgaatgccgt accaacctgc ctaacgtatg ggcaatcggc
    23101 gacgtggttc gcggcccgat gttggcacac aaagccagcg acgaaggcgt tgccgttgcc
    23161 gaacgcattg ccggtcaaaa accgcatatc gacttcaaca acgtaccgtt cgtgatttac
    23221 accgatcctg aaatcgcttg ggtgggtaaa accgaagagc agctcaaagc cgaaggcgtg
    23281 gagtacaaaa aaggtacttc aggttttggt gcgaatggtc gcgcattggc aatgggcaaa
    23341 gccaaaggta cggttaaagt gttggcagac gccaaaaccg accgcatctt gggcgtacac
    23401 atgattggtc cggttgtcag cgaattggtt accgaaggcg tgactgcgct cgaattcttc
    23461 gccagcagcg aagacatcgc ccgcattatc catgcccacc caaccttgtc cgaagtggtt
    23521 cacgaagctg cattggcggc cgacaaacgc gctttgcacg gttgatagac attaaggcct
    23581 ccgacaaatt gaatgttccg agagcttcgt tttctgattt ataattccgt caggcaaaca
    23641 aacagcattt acattcatta tgaacaaaga aatagtcggt gttttcttta taccggcggg
    23701 catcatcagc atgtgtatgg ccgcattgtg gcagatgtat gtgatgatga ccgaaactta
    23761 tacgctcaac cgtttcaaag ataaagaatt ggtttggcgc gtggcattgt tgtttatcag
    23821 tttcagcctt gccgtttacc tgctctgtcc gaattcgcgt aaaaaaggca tcgtcttttt
    23881 tattctcggg ggaggcggcg caatcatgta tctgctggcg cggatgtggt tgcctttcag
    23941 taaatagtag ggccgtctga aaatatggga ttggccgcag ggcgattcct aagacccact
    24001 caccttaagg agaaatccat gaatctacac gagtatcagg ctaaagaact gctggctggc
    24061 tacggtttgc ccgtacaagg cggtattttg gcacgcaacg gcgaagaagc cgctgcagct
    24121 tacgacaaat tgggcggtaa gtttgccgtt gtcaaagcac aagtacacgc cggcggccgc
    24181 ggtaaagcgg gcggcgtaaa agtcgtcaaa agccgcgaag aagccaaaga agtggctgaa
    24241 agcctgattg gcaccaactt ggtaacttac caaaccgatg ccaacggcca acctgtcaac
    24301 agcgttttgg tttgcgaaga catgtatcct gttcaaaccg agctgtattt gggcgcagtg
    24361 gtcgatcgtt ctacccgccg cattacattc atggcttcta ccgaaggcgg cgtggaaatt
    24421 gaaaaagttg ctgccgaaac tcctgaaaaa atcttcaaag taaccgttga tcctctggtc
    24481 ggcctgcaac cttgccaagc gcgcgaagtg gcgttccaac tgggcttgaa agacaaacaa
    24541 atcaacgagt tcgttaaact gatgaccggt gcgtacaaag cgtttgtcga caacgacttc
    24601 gccctgtttg aagtaaaccc gctggcagtt cgcgaaaacg gcgcactggc ttgcgtggac
    24661 ggcaaaatcg gcatcgacag caacgcgctc taccgcctgc caaaaatcgc tgaattgcgc
    24721 gacaaatctc aagaaaacga acgtgagctg aaagcttctg aattcgacct gaactatgtt
    24781 gccctggaag gcaacattgg ctgtatggtt aatggtgcag gtttggcgat ggccactatg
    24841 gacatcatca aactcaaagg cggccaacct gccaacttct tggacgttgg cggtggcgca
    24901 accaaagacc gcgtggttga agcgttcaaa ctgattttgg aagacaaatc cgttaaaggc
    24961 gtattgatca acatcttcgg cggtatcgta cgttgcgaca tgattgcgga agccatcgtg
    25021 gcagccgtta aagaaatcaa cgtcaacgtt cctgtcgttg ttcgtttgga aggtaacaac
    25081 gccgaactcg gcgcgaaaat cctgaacgaa tcaggtctga aactgacttc tgcagacggc
    25141 ctgaatgacg cagccgaaaa aattgttgca gccgtaaacg cctaaggaga aaagaatgag
    25201 cgtattgatt aataaagaca ccaaagtatt ggttcaaggt ttcaccggta aaaacggtac
    25261 tttccactcc gaacaagctc tggcttacgg cactaaagtt gtcggcggcg ttaccccagg
    25321 caaaggcggt caaacccacc tgaacctgcc tgtattcaac accatgaaag aagccgttaa
    25381 agaaaccggc gcggacgcat ctgtcattta cgttccagct ccgtttgttt tggactctat
    25441 cgttgaagcc gttgattcag gcgtaggctt ggtcgttgtg attaccgaag gcgtgcccac
    25501 tttggacatg ctcaaagcca aacgttactt ggaaactaac ggcaacggta cccgcttggt
    25561 cggccctaac tgcccgggcg tgattactcc gggtgagtgc aaaatcggta ttatgccggg
    25621 ccacatccac actccgggcc gcatcggtat tatttcccgt tccggtacat tgacttacga
    25681 agctgtggca caaaccacca aactgggctt gggtcaatca acctgtatcg gtatcggcgg
    25741 cgacccgatt cctggtatga atcaaatcga cgcactgaaa ctcttccaag aagacccgga
    25801 taccgacgcc atcatcatga tcggtgaaat cggcggtact gcggaagaag aagcagccga
    25861 atacatccaa tccaacgtaa ccaaacctgt tgtaggttac attgctggtg ttaccgctcc
    25921 taaaggcaaa cgcatgggcc acgctggtgc gattatttcc ggcggcaaag gtactgccga
    25981 ggaaaaattc gccgcgtttg aaaaagccgg tatcgcctat actcgcagcc ctgccgagtt
    26041 gggcacgacg atgctggaag tgttgaaagc aaaaggtttg gcataatcag gtttgacaac
    26101 tgattgaaca tcaaatgccg tctgaaaccg gaaatcgggt ttcagacggc attttgtttg
    26161 tcatttcaaa aagaggcagc ctcaacatac ccacattaat ttttgccctt tgaggcagtc
    26221 agaatattta tcggtcaaac aggagcaaac ccgtaagtca ggttgcacac gccttgtttg
    26281 aagtcaggga atgataagta tcgcatttga aaagactgat attatttttt gcaggattgt
    26341 tccggtttct gtttcagccc gcgatattct ttgtttgata ggattggaat ggatatagtc
    26401 cgcacgatgc tgcaaatccg tttcatcgcg cacggtgtgt tcgtaaaaac gccgttgtca
    26461 aataccgtgt tcgtgccgcc gctgtttgcc ggcggataag tttttagcat gaggggaata
    26521 tgcggagaat ttggttttaa tcagccgccg gagtagggaa taatccgcat cgtcgggtgg
    26581 cagcgtccaa atggcgtgaa tgtggttcgg cagcacgcat acggcgacgg tttcaaaggg
    26641 atattgtttt tgcacatcca tataagctgc ccgcgaagtc gatatgttcg acaagcaggc
    26701 gcgatttcgg gtcggcgagt ttgacggtga aaaagaatgt gccgccggca atgaagtttc
    26761 tgcgataacg cgccatagga tttttgtttg tggtttgggt gttggagaag gaatggtttg
    26821 ttgtttaagg ttttaacttt gttggagctg atgttttcag acgacattgg aggccgtctg
    26881 aaagcgctag cttcaacgaa gttaaaagtt gaaacggcaa accgtcaatc aagcaaccgc
    26941 gtgcgtgcgt accgcacaca ccctacacgt tgattttgaa gttccgtgta attcacaggt
    27001 agggtgtgtg gcgcagccac gcacgcggtg ttgggtatgc aggctacggc ttgctgctgc
    27061 agaaactcct gaagaaaaag ccaaagacgc cgctgaagag gctaaagaag aagcaaaaga
    27121 cgcagcggaa gatgccaaag aagctgctga agacgctaaa gaagaagcta aataattcgg
    27181 atagctttac agaaaaacgg attccaatga ttgggaatcc gttttttgtt ttgtgagtta
    27241 gggtagttgt tttagataaa gctttttcag ttttcagacg gccatttttc atgccgtctg
    27301 aaagtgttag ttttaactaa gttcaattta caacagaaag ccatcaatca aacaaccgcg
    27361 tgtgtggcgc agccacgcac gcggtcggtt ggatatgtag gctacggctt gctacgtttt
    27421 ttatcaaagg caggcgctaa gtgttcagca ttgttgatgc cgtataaaac taaattatct
    27481 aacagccata atctaaaagc aggatcatcg atatgatgat ctgacgaaca atcgactcgc
    27541 cattgctgta aaacatatcc gatatttgaa gctctaagcg ccaattctaa ttttccattt
    27601 gtcattccaa agtccatttc aatcactttt ttagcttcat catgattagg atggggaacc
    27661 aactcgattt taacaattct attccaatca atatcattgt taatcgtttc attttcttca
    27721 atattttcag ctaataaaat atttgcttga attattcgtg ttaggacaaa atctgcaaat
    27781 cggttttttg ttctatcgaa tgcccgtaaa tgccatcgaa atccgtcatt aactagagca
    27841 agaggcacaa taactttctc agataatcca gatgaaattg aatgatatga gatttttaga
    27901 ggttttttta aggataatgc tttcgatatt ttagaaaaga ttaagacatc aggatttatt
    27961 aaaacaggag gtaaatcaaa cggaagaaga ggttgatgtt gggaatcaat atcaaatatt
    28021 aaactgccat taagaatttt aattgcattt tcaaatgaat gggggaattg aggttcaaag
    28081 tcttcagtag agaggtaaaa tttacttttg gtatcaaaac ggagattttt cgaaaaacta
    28141 gagcaatata gcgcaaaatc cctagtagca acagctgggg aaatttgaaa tgcctgcatc
    28201 aaatcttttc tatttatctt tcctagaaag catagataga aatcaataaa tataatccta
    28261 ttgcgttgag attggttcaa ctctggcata aggggcttta tatgggaagg agcgattgtt
    28321 ttcataattg cattatattc catcaatctt tttcatggca atatgaattc aaaaaaattg
    28381 cttgacgtaa tcaaaaagag tgcatttaat gcaaaaagct acttcatagt agctctctga
    28441 cttaaaattt ctgttatgta attttttata ggatttattt atgaaaataa aatttgctca
    28501 cttaagacat ccagccgtag gtggaggata tattaacttt gctgtttttg atgctaaatc
    28561 aactaatggt aaccatagtg tcgtactgaa ggaattgacc actgaagctc gtagaaatgg
    28621 attaaaaatt gaccaatctg cgttaatatt tgaacaacat gggcgtacac aattctatgg
    28681 agacaaaaat ctagtagatt ttttatctgc aaattggtat ccgcaatgga ctcatacttt
    28741 aactatttaa attatggaga tgataatgac gaacaaaatc aaacctggtc aaaatacagg
    28801 aaataacggt ggaatttatc aagaaattgg ttcacgaggt ggtcgaaaag ataactacgc
    28861 aaccattaaa gataatcaaa aagcaccacc cactcaaaat ccaggaagct catggattca
    28921 aattaaaaga actccagata gctccagata ataaggatag ttctaatgtt tttaggtatt
    28981 tttatagctt tatcaggcat tcttacatta ttagaaatgt ttaatgttat ttcttcggat
    29041 atgaaatggg ggatgccttt aatgttcacc tgtttgggat ttagcattat ttttgatagc
    29101 ttcaaagcca aacaaaatcg aaaacagaag gaataaaaaa acgggatggt ttaaaccatc
    29161 ccgtttttta aagtatattc ttcaaatact tcccagtata actcccctta accttcgcca
    29221 cttcctccgg gctacctgaa gcaataatcc tccctccgcc gtcgccgcct tctggcccta
    29281 agtccacaat ccaatcggcg gttttaatca catccagatt atgctcgata atcactatcg
    29341 agttgccttt gcctttcaga cgacctatga cttccagcag cagggcgatg tcggcgaagt
    29401 gcaggccggt ggtgggttcg tcgaggatat agagcgttct gccggtgtcg cgtttggaga
    29461 gttccaaggc gagtttgacg cgctgggctt cgccgccgga gagggtggtg gcggattggc
    29521 cgaggcggat gtagccgagg cctacgtcca tcagggtttg cagtttgcgc gatacggtgg
    29581 ggacggcgtc gaaaaattcg cgggcttctt caacggtcat gtcgaggact tggctgatgt
    29641 ttttgccttt gtattggatt tcgagcgttt cgcggttgta gcgtttgccg tggcagactt
    29701 cgcagggaac atacacgtcg ggcaggaagt gcatttcgac tttgatgacg ccgtcgcctt
    29761 ggcaggcttc gcagcggccg cctttgacgt tgaaggagaa tctgccgaca ttgtagccgc
    29821 gttcgcgcga gaggggtacg ccggcgaaga gttcgcggat gggggtgaac aggccggtgt
    29881 aggtggcggg attggagcgt ggggtgcggc cgatggggga ttggtcgacg ttgatgactt
    29941 tgtcgaggtg ttcgaggccg cggatgtcgt cgtatggggc gggttcttct tgggcgcggt
    30001 tgagttcgcg ggcggtgatt ttggcgaggg tgtcgttaat cagggtggac ttgccgctgc
    30061 cggatacgcc ggtgatgcag gtaatcaaac cgagcggcag ttcgagggta acgtttttga
    30121 ggttgttgcc gcgcgcgcct ttgaggacga gcattcggtc gggattgacg ggtgtgcgtt
    30181 cagacggcac ggcaatggat tttttgccgc tgaggtattg tccggtaacg gagttttcac
    30241 atttggcgac gttttcgggc gtgtcggcaa tcaatacgtt gccgccgtgt tcgcctgcgc
    30301 ccgggcccat atcgacgacg aaatcggctt cgcggatggc gtcttcgtcg tgttcgacta
    30361 caatcacgct gttgcctaaa tcgcgcaggc gtttgagggt ggcgagcagg cggtcgttgt
    30421 cgcgctggtg caggccgatg gagggttcgt ccaaaacgta catcacgccg gtcaggccgc
    30481 tgccgatttg gctggcgagg cggatacgct gggcttcgcc gccggagagg gtttcggcgg
    30541 agcggcttaa attcaggtaa tccagcccga cgttaatcaa aaagccaagc cgctcggtga
    30601 tttctttgag gattttttcg gcgatttgtt ttttgttgcc gtccaaatcc agcgtttcaa
    30661 agaattggtg ggttttggtc agcggccagg cggagacttc gtgcaacggt tcgccgctga
    30721 cgtaaacgta gcgcgcttct ttgcgcaaac gtgcaccgcc gcagctcggg caggcgcggt
    30781 ggttttggta ttcgcgcaat ttttcgcgca cggtttcgct gtcggtttcg cggtagcggc
    30841 gttcgagatt gggaatgatg ccttcgaagg cgtggctgcg gttgaaggtg gtgccgcgtt
    30901 cggataggta ggtgaaatca atgacttcct tgcccgaacc gtgcaacacg acttttttga
    30961 tttttgcagg tagggtttcc caagcagcct gcacatcaaa accgtaatgc cgtgccagcg
    31021 actgaatcat ttggaaatag aactggttgc gtttgtccca gccgtcaatc gcgcccgaag
    31081 ccagcgacaa ttcggggtgg gcgaccactt tttcggggtc gaagaaattg gtgttgccca
    31141 agccgtcgca agtcgggcag gaacccatcg ggttgttgaa ggaaaacagg cgcggctcta
    31201 attctggcag gctgtacgaa catacggggc aggcgaaacg cgcggaaaac caatgttctt
    31261 cgccgctgtc catctccatc gccagcgcgc gctcgttgcc gtggcgtaac gcggtttcaa
    31321 aactttccgc cagccgctgc ttgatgtccg ccttcacttt tacacggtcg atgaccacgt
    31381 cgatattgtg cttgatgttt ttttccagct tcggcacttc gtccaactga tagacttcgc
    31441 cgtccacgcg cacgcgcgca aaaccctgcg cctgcaagtc ggcaaagaaa tcgacaaact
    31501 cgcccttacg ctctcgtacc gccggcgcca gaatcatcac gcgcgtgtct tccggcagtt
    31561 ttaagacggc atcaaccatt tgagatacgg tttggctgga tagcggcagc ttgtgttcgg
    31621 ggcagtaggg tgtgccgacg cgtgcgtaca aaaggcgcag atagtcgtgg atttccgtaa
    31681 ccgtgccgac ggtggaacgc ggattgtggc tggtggattt ctgctcgatg gaaatcgcgg
    31741 gcgacaggcc ttcgatcaaa tcgacatcgg gtttgtccat catctgcaaa aactgccgcg
    31801 cataggcgga caggctctcg acataacggc gttggccttc ggcatataaa gtatcaaacg
    31861 ctagcgacga tttgccgctg cccgacaatc ctgttaccac cacgagcttg tggcgcggaa
    31921 tgtccaaatc gacgtttttc aaattatgcg tgcgcgcgcc gcggatgcgg atggtgtcgt
    31981 tgccgtgcga atgtttggga tgatggttgc acataatgga tgccgcctga aaaataaagg
    32041 aaaaccgata ttgtagcact ttctcggatg ccgtctgaag ccgcgttcag acggcatttg
    32101 tcggcggagt acggcagatt ccgctataat gtcggcaatt ttaacccgct tgaacaaaag
    32161 gatgacaaat gaaccgtctt tacccccacc cgattatcgc ccgtgagggc tggccgatta
    32221 ttggcggcgg tttggctttg agcctgctgg tgtcgatgtg ttgcggctgg tggtctttgc
    32281 cgttttgggt gtttaccgta tttgccctgc agtttttccg cgaccctgcg cgtgagattc
    32341 cgcaaaatcc tgaagcagtg ttgagtccgg ttgacggccg tatcgtggtg gtcgagcgcg
    32401 cacgcgatcc gtatcgtgat gtcgatgctt tgaaaatcag tatttttatg aacgtgttca
    32461 acgtgcattc gcaaaaatcg cctgccgatt gtacggtaac gaaagtggtc tataacaaag
    32521 gcaaattcgt gaatgcggat ttggacaaag ccagcacgga aaacgaacgt aatgcggtgt
    32581 tggcgactac ggcttccggt cgtgaaatta cttttgttca agtggccggt ttggtggcgc
    32641 gccgtatttt gtgctacacc caagcaggtg cgaaactgtc tcgcggcgaa cgctatggct
    32701 ttatccgttt tggctcgcgc gtggatatgt atctgcctgt cgatgcgcag gcgcaagtgg
    32761 cgattggcga taaagtaaac ggtgtcagca ctgtattggc gcgtttgccg ctgactgcgc
    32821 cgcaaatcga atccgagcct gaatctgagc ctgctttaca aactgctccg gttgaaacag
    32881 cggcaaaccc atctgccgaa caacggcaaa tcgaggcagt ggcggctaag attcaggcgg
    32941 ctgtgcaaga tgtgttgaaa gattaatttt gcgaactgaa atagaaaata tcagtatcat
    33001 cattcacacg aatgaggaag tttggttttt tgaatttttg ctaatgttca caccgttatg
    33061 ttcacgaaaa tgggaatccg ggaacttaac gttacagcga tttatcggaa acggctgaaa
    33121 ccgaacgaat tggattcccg cctgcgcggg aatgacaact cattagttac ctaaaactta
    33181 aaaaacggaa acctttacgc cgtcattccc acgaaagtgg gaatccggga acttaacgtt
    33241 acagtgattt atcggaaacg gctgaaaccg aacgaattgg attcccgcct gcgcgggaat
    33301 gacggcacat tagttaccta aaacttaaaa aaaaaaaaaa acagaaacct ttccgcctca
    33361 ttcccacgaa agtgggaatc tagaacccaa atgctaaggc gatttatcgg aaacggctga
    33421 aaccgaacga attggattcc cgcctgcgcg ggaatgacga cttattagtt acctaacact
    33481 taaaaaacag aaacctttcc gcgtcattcc cacgaaagtg ggaatctaga acccaaatgc
    33541 taaggcgatt tatcggaaac gactgaaacc gaatgaattg gattcccgcc tgcgtgggaa
    33601 tgacgaccca ttagttacct aaaatttaaa aaacagaaac ctttccgcgt cattcccatg
    33661 aaagtgggaa tctagaaccc aaatgctaag gcgatttatc ggaaacggct gaaaccgaag
    33721 agaccggatt cccgcctgcg cgggaatgat gggtttctgc ttttgatttt tctgcttttg
    33781 cgagaatgac ggcgtgaaag caagaatgat gaaacaaaga aaatgggaat gatggcatag
    33841 tggtttgttc tttgtctttg ccatatttcc taacaaattg attaaaaaga aaaaaggttt
    33901 tcagaatgcc gtctgaaaac cttttttgtt tgcctgtccg attttaaaac ttcacgttca
    33961 cgccgccggt aaagctgcgg cccatttgcg gcgtatcaga gaggaagctg ctgtgggcgt
    34021 aaacggattg gttgagcagg ttgtcggctt tgacgtacca attccactcg ccatagcgcg
    34081 tattgcggcg gtagtttgcg ccgaggttga gcatatggtg tccgggcgtg cgcgtttcgt
    34141 agcgggcgag tttgttttgg gcgaacacgc ggtagtagtc caaattggca tcgatgcggt
    34201 cggtcagcga ggctttcagg tggacgccga ggcgcgcagc cggaacgcga ggggcgtttt
    34261 ggtcgtcctg cgcgatgaaa ggacggttgc cgtaggcatc ttccctgccg ggtagggaag
    34321 gcaggttttt cagacggcct cgtacatagt cgccggaaac gccgatgcgg tagcgcggtg
    34381 tcggtttgaa gtagatttct ccttccgcgc cgtagaagtc ggcgccggat tggttgtagc
    34441 gcacgagctt catttcgctg tcgtcttcga tggatttggg gccgcgtcca tcgtttaagg
    34501 tttgggcgta aatgtagttg ccgaagcggt tgcggtagag tgccagattg tattgccagc
    34561 ggtcgccttc gtagcccaac gcgagttcga tattgttgga acgctctttg ttcaggtgtt
    34621 tgttgccgac ttcaaaagtg ttggtggcaa cgtgtttgcc gtgtgcgtac agctcttgcg
    34681 ttgacggcag gcgttcctga tgggaggcgg tcaggctgag tttgtgttgc ggcgtgaaat
    34741 accagttgcc cgaaagtgcg aacgagcggg cggtttggcg gtgtgcgccg aggtcgggca
    34801 ggggctgctt gtagtagttt tcccgatcaa tcaatgcttt gtcgtactgg atggaagctt
    34861 tttgtttttc cacgcgtacg ccgccttcaa gcgtgaagtt gtcccattct gcctgttcta
    34921 cgccgaaaaa gctgtaatgt tgcactttat tgtcaagcag catcggttgt ttaaccgctt
    34981 cggatatggc agataaagca ctggattttt gttgtaaata ttgcacgccc cagctgcctt
    35041 tcagacggcc tatgggttgg tggcgcaact cgatacgggc gttttgcgtt ttgttgttga
    35101 agaagttttc gaccgcatcg cctgcttttt cgtcgtggtg gtagtcgttg cggttcaggt
    35161 gcacgcgcag ggcttcaaaa ccggggagcg gctgcttcca ttcggcgcgg agttcgtagc
    35221 gtttgttgcg caggtctatc catggtttgc cgttgtgggt atgggcgtgt gcattatcgt
    35281 cgtcgtggaa gccgcagctc aaacccggat tgtcataatc gacgtcttct tcggtcaaca
    35341 ggtgcggata aagctgcaaa tagcgtttgt taatcaaact cttttgccag atgatgtcgg
    35401 cgtggcaatc atcgtattcg tggctgtggg caggcaggcc gtattggtct cggcggtcgc
    35461 tgtacgccgc gccgataaag cctttttcgc ccacccaaga cagcccgatg ctgcctgttt
    35521 ttgaatcggc atggctgtcg ggcaggcgtt tcagattgcg gtaacgcggt acggcgtaat
    35581 cgcccgattt gcggtacaag ccttcggtat gcaatacaaa gtttttgccc aagccgatat
    35641 tgatgccgcc ggacgtgagt ttttccagat tgccgctgct caaacgcaat ccgagttcgc
    35701 ccgatacgcc gttttcaggc attttttcgg ggattttgcc atcggcaaca tcgaccagcc
    35761 ccgccacatt gcccgagctg tacaagagcg taaccggccc gcgcaggatt tcgacctgtt
    35821 gcgacaaggc ggtatctacc ataatggcgt gatcgggaga aaaatccgcc atatcgcctg
    35881 tttcgccgtg atggttcaac actttaatcc gcctgcctgt ttgaccgcga ataacgggag
    35941 cggacgcgcc gccgccgtat tgcgaggcgt gaatgcccgg cacgccgtcc aaagcatcgc
    36001 ccaagttgac ggctttttgt cgcaaggtgt cgccgctgat gattttgtcg gaggcggtcg
    36061 aagtgtgcag cagcccggat gtggcgcgcg gacggctttt gccgacgacg gtaaccgttt
    36121 ccaagcccac cgattgctca gtttcatgcg cttgggcgag gaggggtgtg ttgattaaaa
    36181 gaattgataa aacaatgggt ttgagtgtag tttgtgccat tttggcttct cgtcgcattt
    36241 caaaagtttg ttattatata acattacatt ttttatatca taagattttg agaacactca
    36301 gagggcatag gcaaaagttt ttcaaatgaa acggttgcgg catcgggcgg tgtccatttg
    36361 tatccgccgt ccttcggggg cgcggttgat gttgacgcag attccgctgt gtttgcccat
    36421 tatgttcggc tgcgggataa ccaaaattta tgagtgcata aaaacggcac gttcccgaac
    36481 ggtcgaaaag gtggcaaaat ggcgtacttg tcagaacgcg aggctctgcg ccaggttcac
    36541 gagggcgcat cgggcacgct aagatataag ggggtatgga tgggggtatc ggaaatgcag
    36601 ttaataataa acaaattata aatcaatagg ttaatcacaa aatgcttttg tttatcgaca
    36661 attacgacag ttttacttac aacatcgtcc agtatttcac tgaattgggg caggaagttg
    36721 ctgtgcgccg caacgatgat attacgttgg aggaaatcga agccttaaag ccgcaatatc
    36781 ttgtaatcgg ccccggaccg tgttccccta aggaggcagg gatttcagta gaagccatgc
    36841 gccattttgc cggccggctg ccgattatgg gcgtgtgcct cgggcatcag acgataggcg
    36901 aggcgttcgg cggcaggata gtccgcgcca aaacgctgat gcacggtaag gtgtcgcccg
    36961 tgtcccattc gggcaagggg atgtttaagg gtttgcccaa tccggttacc tgtacgcgtt
    37021 atcacagcct cgttattgaa cgcggcacac tgcccgaatg tttggaaatc acggcatgga
    37081 cggaagacgg cgagattatg ggcgtgcgcc ataaggaata tgccgtcgag ggggtacaat
    37141 tccaccctga agctctgctg accgaacacg gacatgatat gttaaacaat tttttagttg
    37201 aatttcaaaa ctttaaaccg caaaaaatct gacgtgatgc cgtctgaatc ccttcagacg
    37261 gcattttcgt ccgaatattg aacggaggac aaaaaatgat tacaccgcaa caggctatcg
    37321 aacgattaat cagcaataac gagttgtttt acgatgaaat gaccgacttg atgcggcaaa
    37381 tgatgagcgg aaaagtgccg cccgaacaaa ttgcggcgat tttgacagga ttgcgtatca
    37441 aggttgaaac cgtttccgaa attaccgccg ccgccgccgt catgcgcgag tttgcgacaa
    37501 aagtgccgct ggagaatgca gaggggctgg tcgatatcgt cggtacgggc ggggatggcg
    37561 cgaaaacctt caatatttcg acgacttcga tgtttgttgc cgcagcggca ggcgcgaagg
    37621 ttgccaaaca cggaggccgg tcggtctctt cctccagcgg tgcggctgac gtggtggagc
    37681 agatgggcgc aaacctcaac ctgactcccg aacaggttgc ccaaagtatc aggcagaccg
    37741 gcatcgggtt tatgttcgcg cccaatcacc acagtgccat gcgccatgtc gcccctgtac
    37801 gccgttcgct cggtttccga agtattttca acatattggg tccgttaacg aatcctgcgg
    37861 gcgcgccgaa ccagcttttg ggcgtgttcc acaccgattt gtgcggcatt ttgtcgcggg
    37921 tcttgcaaca acttggttca aaacacgttt tggttgtttg tggggagggc ggtttggatg
    37981 aaattacact gacaggcaaa acgcgcgttg ccgaactcaa agacggaaaa atcagcgaat
    38041 acgacatccg cccagaagat ttcggtatcg aaacccgccg caatttggat gaaatcaaag
    38101 ttgccaatac tcaggaatct ttgttgaaaa tgaatgaggt gctggaagga agagaagggg
    38161 ctgcgcgcga tatcgtattg ctcaacaccg ccgccgccct gtatgccgga aatgtcgctg
    38221 cttcgctttc agacggcata tctgccgcac gggaagccat cgattcaggc agggccaaat
    38281 cgaaaaaaga ggagtttgtc ggttttcaac cacaacaaag atgccatttt cttggaaaga
    38341 tggagcttgg gtgatgccgc catgattatg gaacttttgt ggcaaaacat aagcacttca
    38401 cgaagagaac ttaccaaact gtttttatat aaaaacttgg ggctgtacta gataaccaga
    38461 ccaaattccc attaactaat tgtcttaaaa tctgaatttg agattctatt taaaatgcca
    38521 ttggcatttc tttaaatgca gccccaaatg ctctttggga atgccgttaa acttacgtaa
    38581 atggctaaat tcactaatat caagcacatc ataactacga aaggtatccg tgtatacaat
    38641 gccatcaggc ttaactttct ttcggataat tggcaacaat gtcgctgatt gcgcattagg
    38701 aacaacgacg gta
//
LOCUS       DAWFIE010000009        38112 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWFIE010000009 DAWFIE010000000
VERSION     DAWFIE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.009"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            50..322
                     /locus_tag="WGQ71_000418"
     CDS             50..322
                     /locus_tag="WGQ71_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706486.1"
                     /translation="MMSVKELFKVILDENKDFPIRTIHRTPIGILPKPVALSIVQYED
                     DQGFYLFYLDETGQEQTDTYHDTLDSAFEQVEFEFGISKEEWMQSP"
     gene            304..456
                     /locus_tag="WGQ71_000419"
     CDS             304..456
                     /locus_tag="WGQ71_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706487.1"
                     /translation="MDAKPLGGNIKMPLYFSDGISVNKKVGIGVQNSLHLAGIGMKSL
                     RKNDYS"
     gene            443..988
                     /locus_tag="WGQ71_000420"
     CDS             443..988
                     /locus_tag="WGQ71_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0706488.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAEWVEKKGWNAFE
                     QFLLDNEVGNLSDMNRKPFTW"
     gene            1124..1801
                     /locus_tag="WGQ71_000421"
     CDS             1124..1801
                     /locus_tag="WGQ71_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BECR-fold containing toxin"
                     /protein_id="HEZ0706489.1"
                     /translation="MVKTADGYKAIAHIQTGEHVFAKDETSGKTGYKPVTARYGNPYQ
                     ETVYIEVSDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLFAESGKTQTVRNIIV
                     KPKPLKAYNLTVADWHTYFVKGNQAETEGVWVHNACPPRKTPSTPVYGNDSEAYAAAK
                     KLGYRKIKERTRNDAAIFKKGKSYISRDVDSHNGGAWKEASSPKNLNRKETRNGTFDK
                     NLNRIGD"
     gene            1807..2205
                     /locus_tag="WGQ71_000422"
     CDS             1807..2205
                     /locus_tag="WGQ71_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0706490.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDVEDWKFFYFPIQHSKGRLR"
     gene            2236..2613
                     /locus_tag="WGQ71_000423"
     CDS             2236..2613
                     /locus_tag="WGQ71_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0706491.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIEFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVQFDYYNKK"
     gene            2649..2747
                     /locus_tag="WGQ71_000424"
                     /pseudo
     CDS             2649..2747
                     /locus_tag="WGQ71_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3018..4151
                     /locus_tag="WGQ71_000425"
     CDS             3018..4151
                     /locus_tag="WGQ71_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706492.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIMKESMRTEESLAGAVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDYVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQDFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKRGDRLPDFPLNLENLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(4253..4528)
                     /locus_tag="WGQ71_000426"
     CDS             complement(4253..4528)
                     /locus_tag="WGQ71_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706493.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKIRLMCISVFWIVVFNL"
     gene            complement(4545..5312)
                     /locus_tag="WGQ71_000427"
     CDS             complement(4545..5312)
                     /locus_tag="WGQ71_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0706494.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCKPVNEQTSFNNPEPMTGFE
                     HTVTFDFQGTKMVIPYGYLARYTQNNATKWLSDTPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACHLNQDLGKSLLDLGFKKIPSVYPE
                     YEAYEDKRSIPENPYIHELQYIKKGENPAIITHQNYHRYGENDYSTDIGSCINGFTVQ
                     YYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            complement(6256..6531)
                     /locus_tag="WGQ71_000428"
     CDS             complement(6256..6531)
                     /locus_tag="WGQ71_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0706495.1"
                     /translation="MNALNWSLYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(6528..7811)
                     /locus_tag="WGQ71_000429"
     CDS             complement(6528..7811)
                     /locus_tag="WGQ71_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0706496.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWIFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(7959..9377)
                     /gene="glnA"
                     /locus_tag="WGQ71_000430"
     CDS             complement(7959..9377)
                     /gene="glnA"
                     /locus_tag="WGQ71_000430"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ0706497.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDTTVVFTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            complement(9651..10028)
                     /locus_tag="WGQ71_000431"
     CDS             complement(9651..10028)
                     /locus_tag="WGQ71_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706498.1"
                     /translation="MKKMLCLAVFALGLATPAVAADWVLVDSNTATFGDADSRTDNSA
                     WFETRFAKPQKYGNGKFFNTAKTLEEMDCSGKRFRILTATWYSKSGNSIASYTPSYAE
                     WNYVIPGTLGEAQYKFVCNGYPR"
     gene            10198..11007
                     /gene="aroE"
                     /locus_tag="WGQ71_000432"
     CDS             10198..11007
                     /gene="aroE"
                     /locus_tag="WGQ71_000432"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ0706499.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFGTGGCGANVTVPFKQEAFHLADEHSDRALAAGAVNTLILLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAM"
     gene            11009..11710
                     /gene="mtgA"
                     /locus_tag="WGQ71_000433"
     CDS             11009..11710
                     /gene="mtgA"
                     /locus_tag="WGQ71_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ0706500.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLSKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(11912..11987)
                     /locus_tag="WGQ71_000434"
     tRNA            complement(11912..11987)
                     /locus_tag="WGQ71_000434"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11952..11954),aa:Phe,seq:gaa)
     gene            complement(12178..12912)
                     /gene="lptB"
                     /locus_tag="WGQ71_000435"
     CDS             complement(12178..12912)
                     /gene="lptB"
                     /locus_tag="WGQ71_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ0706501.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEISTKDKSRIDGEVEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRAVYLGENFKY"
     gene            complement(12986..13516)
                     /gene="lptA"
                     /locus_tag="WGQ71_000436"
     CDS             complement(12986..13516)
                     /gene="lptA"
                     /locus_tag="WGQ71_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ0706502.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLNQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRNDKGEQFMKADGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVIIQPSSTQKSE"
     gene            complement(13497..14078)
                     /gene="lptC"
                     /locus_tag="WGQ71_000437"
     CDS             complement(13497..14078)
                     /gene="lptC"
                     /locus_tag="WGQ71_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ0706503.1"
                     /translation="MKIRWRYGIAFPLILAVALGGLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(14075..14611)
                     /locus_tag="WGQ71_000438"
     CDS             complement(14075..14611)
                     /locus_tag="WGQ71_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0706504.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(14676..15650)
                     /locus_tag="WGQ71_000439"
     CDS             complement(14676..15650)
                     /locus_tag="WGQ71_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ0706505.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKNITLVCITARPDSTMARHADIHITASVSQEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTIAAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            15746..16801
                     /gene="tal"
                     /locus_tag="WGQ71_000440"
     CDS             15746..16801
                     /gene="tal"
                     /locus_tag="WGQ71_000440"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ0706506.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLSPKQRYETMAVADVRAACGICLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRVQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWEQYFTTPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDNL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLANRLQED
                     GLKQFEEAFEKLLAPLV"
     gene            16955..17245
                     /locus_tag="WGQ71_000441"
     CDS             16955..17245
                     /locus_tag="WGQ71_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706507.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYRELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALIHSLK"
     gene            17262..18149
                     /gene="gluQRS"
                     /locus_tag="WGQ71_000442"
     CDS             17262..18149
                     /gene="gluQRS"
                     /locus_tag="WGQ71_000442"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ0706508.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSHRYALYEETLCRLQTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPCQRPAPQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLNLPEAPEADRPAELLD
                     WAVAHWDMGKVPKHAITTP"
     gene            complement(18219..19229)
                     /gene="dusA"
                     /locus_tag="WGQ71_000443"
     CDS             complement(18219..19229)
                     /gene="dusA"
                     /locus_tag="WGQ71_000443"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ0706509.1"
                     /translation="MNDNTHTLPPRRLSVAPMLDWTDRHYRYLARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQTQI
                     QAGRGTILRHIVRHSLGLTHGLKGARTWRRMLSDATLLKDNDGSLILEAWEEVERANM
                     RE"
     gene            complement(19260..19751)
                     /locus_tag="WGQ71_000444"
     CDS             complement(19260..19751)
                     /locus_tag="WGQ71_000444"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ0706510.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGSVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGYGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(19825..20583)
                     /locus_tag="WGQ71_000445"
     CDS             complement(19825..20583)
                     /locus_tag="WGQ71_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0706511.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(20654..21520)
                     /locus_tag="WGQ71_000446"
     CDS             complement(20654..21520)
                     /locus_tag="WGQ71_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0706512.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(21569..21847)
                     /locus_tag="WGQ71_000447"
     CDS             complement(21569..21847)
                     /locus_tag="WGQ71_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0706513.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(21847..22137)
                     /locus_tag="WGQ71_000448"
     CDS             complement(21847..22137)
                     /locus_tag="WGQ71_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ0706514.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(22140..22670)
                     /locus_tag="WGQ71_000449"
     CDS             complement(22140..22670)
                     /locus_tag="WGQ71_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ0706515.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            22978..25503
                     /locus_tag="WGQ71_000450"
     CDS             22978..25503
                     /locus_tag="WGQ71_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ0706516.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETV
                     AETPDFNATADDLSALLQPSKVPAVEENAAETVADDLSALLQPAEAPAVEENVTETVA
                     ETPDFNATADDLSALLQPSEAPAVEENAAETVADDLSALLQPAEAPAVEENAAEITLE
                     TPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTSFPSESVGSDAPSEAKY
                     DLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(25675..26091)
                     /gene="gloA"
                     /locus_tag="WGQ71_000451"
     CDS             complement(25675..26091)
                     /gene="gloA"
                     /locus_tag="WGQ71_000451"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ0706517.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(26116..27285)
                     /gene="lapB"
                     /locus_tag="WGQ71_000452"
     CDS             complement(26116..27285)
                     /gene="lapB"
                     /locus_tag="WGQ71_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ0706518.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(27296..27610)
                     /locus_tag="WGQ71_000453"
     CDS             complement(27296..27610)
                     /locus_tag="WGQ71_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ0706519.1"
                     /translation="MKLIYTVVKIIILLFFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIIFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESTK
                     QP"
     gene            27920..28918
                     /gene="ilvE"
                     /locus_tag="WGQ71_000454"
     CDS             27920..28918
                     /gene="ilvE"
                     /locus_tag="WGQ71_000454"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ0706520.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLVPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVAELKAAVENGAEA
                     ILTGTAAVISPVTSFVIDGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(29163..29948)
                     /gene="fabI"
                     /locus_tag="WGQ71_000455"
     CDS             complement(29163..29948)
                     /gene="fabI"
                     /locus_tag="WGQ71_000455"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ0706521.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(30103..30924)
                     /gene="dapD"
                     /locus_tag="WGQ71_000456"
     CDS             complement(30103..30924)
                     /gene="dapD"
                     /locus_tag="WGQ71_000456"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ0706522.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPTGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(31134..32777)
                     /gene="pgi"
                     /locus_tag="WGQ71_000457"
     CDS             complement(31134..32777)
                     /gene="pgi"
                     /locus_tag="WGQ71_000457"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0706523.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALEFAHSLDDGSYQGITGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETARHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPLRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            33287..34519
                     /gene="pilG"
                     /locus_tag="WGQ71_000458"
     CDS             33287..34519
                     /gene="pilG"
                     /locus_tag="WGQ71_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ0706524.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            34593..35453
                     /locus_tag="WGQ71_000459"
     CDS             34593..35453
                     /locus_tag="WGQ71_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ0706525.1"
                     /translation="MSDLSVLSPFAVPLTAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            35455..36087
                     /locus_tag="WGQ71_000460"
     CDS             35455..36087
                     /locus_tag="WGQ71_000460"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ0706526.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHNG"
     gene            36080..36289
                     /gene="yacG"
                     /locus_tag="WGQ71_000461"
     CDS             36080..36289
                     /gene="yacG"
                     /locus_tag="WGQ71_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ0706527.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADEEYTVTAQEEGLLEISEPDGAYR"
     gene            complement(36374..38050)
                     /gene="pilB"
                     /locus_tag="WGQ71_000462"
     CDS             complement(36374..38050)
                     /gene="pilB"
                     /locus_tag="WGQ71_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ0706528.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         9395 a        10180 c         9521 g         9016 t
ORIGIN      
        1 tggacgtgta atagaaaata gtgcggtaac aattattcgg gattgaagaa tgatgagtgt
       61 aaaagaatta ttcaaagtca tcttagatga aaataaagat tttcctatta gaacgattca
      121 tcgcacgcca atagggatac ttcccaaacc tgttgcttta agtattgttc aatatgaaga
      181 tgaccaagga ttttatttgt tttatttgga tgagaccggt caggaacaaa cggatactta
      241 tcatgacaca ttagattcgg catttgaaca ggtggagttt gaatttggaa ttagcaaaga
      301 agaatggatg caaagccctt aggtggcaat atcaagatgc cgctttattt ttcagacggc
      361 atttccgtca ataaaaaagt cggtattgga gtccaaaaca gcctacatct tgccgggata
      421 gggatgaaat ctttaagaaa aaatgattat agttgaacat ttagaacatt atttgggtga
      481 aatcgaaagc ggtataaaat gcttggatcg acgttatcat ctcagcgttt ccgtcttccc
      541 atcacaacca tataaagggg taactacttt ttctacttta ggcttgaatc gatatgactt
      601 gaactataag agtaggtttg aattaatttt tacctgttct gaagaatgga ataaagaaaa
      661 tattgcagct tttctatcag gagtagccga atatttgatt gacaatagac aacctatttt
      721 aagaggtgaa attattcaat tacccagggt aattatcgaa ggtagcaaaa tggatgcact
      781 gtatgtttcc gccccatttt atttcgacga tgattttcag gtctgttatg gcgaacacta
      841 caatatcgtt ttccctttgc ttgtcccctt gtataaacag gaagccgaat gggtggaaaa
      901 aaagggttgg aatgcttttg agcagttctt gctggataat gaagttggca acctttcgga
      961 tatgaatcgg aaaccgttta cttggtaagg attttcttaa atttaatcaa agtgccgtct
     1021 gaaaccgtgt ttaattttca ggcgaccttc cccttatttt aaacaggata ttgggaacta
     1081 agttcttcaa aaatcctaca cctgctcctt ccacggcagc accttggtca aaacggcaga
     1141 cggctacaaa gccatcgcac atatccaaac tggagagcac gtctttgcca aagatgaaac
     1201 cagtggaaaa actggataca aacctgttac cgcccgatac ggcaatccgt accaggaaac
     1261 cgtttacatt gaagtttcag acggcatcgg caacagccaa accctgattt ccaaccgcat
     1321 ccatccgttt tattcggatg ggaaatggat taaagcggaa gatttgaaag cgggaagcag
     1381 gctgtttgcc gaaagcggca aaacccaaac cgtccgcaac atcattgtca aaccaaaacc
     1441 gctcaaagcc tacaatctga ccgttgccga ctggcatacc tacttcgtca agggtaatca
     1501 ggcggaaacg gaaggggttt gggttcataa tgcgtgtccg ccaagaaaaa caccatctac
     1561 ccccgtatat gggaatgatt cagaagcata tgcagcagca aagaaattgg gatatagaaa
     1621 aataaaagaa agaacgagaa atgatgctgc tatttttaaa aaaggaaaga gttatatatc
     1681 acgcgatgta gattcacata atggaggggc ttggaaagaa gcatcttcac ccaaaaatct
     1741 gaacagaaaa gaaacaagaa acggtacatt tgataaaaac ctaaatcgta ttggagatta
     1801 attattatgt gcgagttcaa ggattttaga agaaacatcc cttgttttga agagtatgac
     1861 gaaaattcat ttattggcaa atggtatgat gacggagtgt gggatgatga agaatattgg
     1921 aaattggaga atgctttaat cgaggttaga aaaaaatatc cttatccgat ggatatacca
     1981 agggacatcg tgattggaat cggtaccatt attgattttt taatggttca aaattggaaa
     2041 ctttttgaaa ttaaagcttc tccttggttg cctaaaagtg tcaaaattaa tgagcgttat
     2101 gaaaggttca gagtaatgct ccgttatatt tttactgatc tagatgtaga ggactggaaa
     2161 tttttttact ttccaataca acatagtaaa ggtagattga gatgaaaagt ttaattatat
     2221 tggagataca ttattatgtg tgagttcaag gatattataa gaaacgttcc ttattttgag
     2281 gggtatgacg aaaattcatt tattggcaaa tggtatgatg acggggtgtg ggatgatgaa
     2341 gaatattgga agttggagaa tgatttaatc gaggttagga gaaaatatcc ttatccgatg
     2401 gatataccaa gggatatcgt gattggaatc ggtaccatta ttgagttttt aatggttcca
     2461 aattggaaac tttttgaaat taaagcttcc ccttggttac ctgatagcgt gggaattaac
     2521 gaacgttatg aaaggcttaa aacaatgctc cgttatattt ttaccgagaa agacatagtc
     2581 aacgtgcaat ttgattatta caacaaaaaa tagcaaaacc cggcaatcaa aatgccgtct
     2641 gaagcggtat tcggctttca gacggcatcg gtaacagcca aaccctgatt tccaaccgca
     2701 tccatccgtt ttattcggac ggcaaatgga ttaaggcaga agacttaata ttggtgggaa
     2761 atggaaacgt tcaaaatatc agttttagaa gattgggaac gctcaggaag accgttaaat
     2821 tgggaaaaat ggcaaaggca atatcgatct taagcatttg aattagttga aaatttgaat
     2881 caaagcaaac aagagtaaca agccgcagcc tgcataggtt tttttaaagt acggcagcaa
     2941 gcgtagatta gattaaaaat caaacaatca aagctcgtct gatataatcg atatattctg
     3001 ttaaacgaaa aataaatatg tttaaaaaat tcaaaccggt actgttgtca ttttttgcac
     3061 ttgtatttgc cttttggctg ggaacgggta ttgcctatga gattaatccg cgttggtttt
     3121 tgagcgatac ggcaactgaa atacctgaaa atccgaatgc ttttgtggcg aaacttgccc
     3181 gcctgttccg aaatgccgac agggcggttg tcatcatgaa ggaatcgatg aggacggagg
     3241 aaagtcttgc cggagctgtg gatgacggtc cgttgcagtc ggagaaggat tatctcgcgc
     3301 tcgctatccg gctcagtcgt ttgaaagaaa aggcgaaatg gtttcacgta acggagcagg
     3361 aacatgggaa agaggtttgg ctggattacc atatcggcga gggcggtttg gttgcggttt
     3421 cgctttcgca acgctcgccg gaagcgtttg ttaatgccga atatctgtat cggaacgatc
     3481 gtccgttttc tgtaaatgtg tacggcggaa cggctcacgg agaaaattat gaaacgacag
     3541 gagaatatcg ggttgtttgg caaccagacg gttcggtatt tgatgcgtcg gggcgcggaa
     3601 aaatcgggga agatgtttat gagcattgtc tcgggtgtta tcagatggcc caggtatatt
     3661 tggcgaaata tcgggatgtc gcgaatgatg agcagaaggt ttgggacttc cgcgaagaga
     3721 gcaaccggat tgcgtcggac tcgcgtgatt atgtgtttta tcagaatatg cgggaattga
     3781 tgccccgggg gatgaaggca aacagtcttg tggtcggcta tgatgcggac ggtctgccgc
     3841 agaaagtcta ttggagtttc gacaatggga aaaaacgcca ggatttcgaa tattatttga
     3901 aaaacggaaa tctttttatt gcacaatctt cgacggtagc attgaaagcg gatggagtaa
     3961 cggcggatat acagacctat catgcgcaac agacgtggta tttggatggc gggcggatta
     4021 tccgcgaaga gaaacgggga gacagactgc ctgattttcc tttgaacttg gaaaatttgg
     4081 aaaaagaggt gagccgttat gcagaggctg cggcgagacg ttcgggcggc aggcgcgacc
     4141 tttctcactg aacgcaaatg ccgtctgaaa ccggttttca gacggcattt tttcagacgg
     4201 cactggagag tcaaacgctt tttgcaatgc cgtatattcc aaatccaatt cgctataaat
     4261 tgaaaaccac aatccaaaaa acggaaatac acattaaacg gatcttttta ccgaccgtat
     4321 catcccatag gttttccaca ccttttttca tccataaatt caaaccattt aagtttgcat
     4381 ccttaccgac aattgcagca gcatcatccg cataacggtc acccaaatta gccaaataac
     4441 gccaaccgcc ataggtatcg ccacggaaag cataaccacg caaggtttgg atttgttctg
     4501 gtgttaaaac aacttcaccc tcattcataa taagatcctt taaattattt tttacttgaa
     4561 ttgtttacaa aactctgtac caattgctct acttgttggt gataacctac caactcctgc
     4621 tgtgtgagct gctgcttttc ccgaataaac gggtaatact gtaccgtgaa accgttaata
     4681 caggaaccta tatcagtact gtaatcgttc tctccatacc tatgatagtt ctgatgagta
     4741 ataatcgccg gattttctcc tttcttaata tactgtagtt catgaatata tgggttttca
     4801 ggaatcgacc ttttatcttc ataagcttca tattcaggat agacagacgg gattttttta
     4861 aatcccaaat caagcaacga ttttcctaag tcttgattca aatgacacgc atcttttcgg
     4921 ataacaatat cgtccacgct atccaaacca tcgcgtagaa attgaataaa gtgtgctttg
     4981 ttctgctggt tgtatggttc gagcacccag ccttggtcgg tttttttgta atagacgcta
     5041 atctctatca aattaatgga gtaagcatcc tgcccgggcg tgtcggaaag ccattttgtg
     5101 gcattgtttt gcgtataccg tgcaagatag ccatagggga taaccatttt ggtgccctga
     5161 aaatcaaatg taaccgtatg ttcaaatcct gtcattggct cgggattgtt gaaactggtt
     5221 tgttcattaa caggtttaca tgaggtcata gtaaaaacac tcccaataaa caaaataaaa
     5281 agttgataaa tggtagtagc atatggtctc ataatttcaa gcttagaaat tagttaaaga
     5341 atatattcat ggtagatact aactctttaa gttgacatat tgtgcgatat cgagggctgt
     5401 accatatttt cccatcgggt cttccccgtt ctttatatga gcggcaggca tcctcataaa
     5461 agaagggata ccgataagga aaaatacctc ggcataaaaa atatactgat taatcaatac
     5521 aaatccaaca aacagtttat cattttcttt tctgttggcg tataaaaacc gcttggaaaa
     5581 aataaaatat caaaacggca aaataaaaaa actgatatat gccttcactt tcatagggcg
     5641 acgctttatg tgccgtcctg tgtgttgaaa catcggtttt cctttttcaa aactccgtaa
     5701 aaaaagcggc atccgccgca aaacgccccg tttttccaaa caaagggcaa taaaaatgcc
     5761 gtctgaaacc aagttcagac ggcatcaagc catcaaatca aaccctgcct cctcatcatc
     5821 cgataagcct catccacatc atcggtttgc agataaatac cgacaacccg cttcaactgc
     5881 tcttcatcat aatcatacgg ctgcatatca tcccgaaact ccacaggaca ataactgtcg
     5941 acagccaaac tcaaagactg ttcgatatag ggaacaggcc atccggcttg ctactgttgg
     6001 caatccaatc gtccataaac cggttgtaaa acgtttcggc atccgttttc ccgtcgaaga
     6061 aatcttgcgc catcttggaa aaaaccgttc ttttcattgc agataatccg tattcaaata
     6121 atgctcgcgc tgttttccac ttatcttcat tgccgttaca aacctattgt ctttatcaaa
     6181 aacgacagcg tcgcctgccc tgctttgtcc tgattgttgc caactgctgt aaccatgccg
     6241 tccgaacctg aaccgtcagg tttcagacgg cattttttct gcctgctccc acaattgccg
     6301 tttttgtgcg gcggtcagtt ttttgacggc gatttcctgc ctgagtgccg tgcctttatc
     6361 catcccgcct gcaacgatac gcatcgccac cggtttgaat acgcgggtat atttcgcgcc
     6421 tttaccggtt gtgtgggcgg caagccgctg ttgcggattc gggctgatgc cgcaatagaa
     6481 cgcgctgttt tcacacaata tcaggtaaag gctccaattt aacgcgttca tctgcctgca
     6541 tcctgagttt tctccccgtt ccaaggcgca actttcagca gcagcagcat acccggtagg
     6601 gcgagtgcga aacacagttg gaaaaacggt acataaccga gccattcaat cagataaccg
     6661 gcaaaggaat tgatgaccgt acggggaaca gcggacaggc tggtaaacag cgcaagctgc
     6721 gttgcggtaa atgcgggatt ggtctcgcgc gccatatacg atacgaacgc cgccgtcccc
     6781 aaccccacgc cgaccgcttc cgcgccgata actgccgcca gcatcagcct ctcgcctgtg
     6841 ccgaccgtgt cgaaatgtcc gaaccctgcc agccatacaa accccaaaac ggttacagcc
     6901 tgcaccgcgc cgaatatcca caaggctttg tttacgccga ttttcagcat ccacacgccg
     6961 cccaagatac ctgccgccac tgccggccac agtcctgcat ttttcgcaat caaaccgatg
     7021 tctgtcttgc tgaaacccat atccagataa aacggcgttg ccaacgcggt tgccatactg
     7081 tcgccgagtt tgtaaaggaa gataaacagc agcacgcata cagccgaagc gatgccttta
     7141 cgtgtaaaaa attctttaaa cggctctacc acggtctgct tcaacgtttt aggaacggca
     7201 ggcggcaaca cgggttcgcg cgcgagaaac agcgtcatca gaaggccggg cagcataaat
     7261 aatgaagtaa taacaaatac ttctgaccac ggcatcctgt ctgccaacac caaactcaat
     7321 gaaccgggaa tcagggcggc aatccggtag gcgttcacat gaaccgagtt gcccaaaccc
     7381 aattcttcgt ccgacaaaat ctcgcgcctg aacgcatcca atacaatatc ctgactggcg
     7441 gaaaaaaaag cgacaagcac cgacaagccg gcaatcagcg gcagatgatt acgggggttt
     7501 aaaaaggcat aggccgccaa agccgccagc aaccctgcct gcgtcagcag catccacccg
     7561 cgccgccgtc ccaaaacggg cagcctgacc gcgtccatca gcggcgacca caaaaatttc
     7621 caagtaaacg gcagaccgat taacgccatc agcccgatgc tcttcaaatc cacctgctcg
     7681 ctgcgcaacc acgccggaat caggttaatc agaaagtaca gcggcagccc cgaggtaaac
     7741 cccgtaaaaa tacagacgag catattgcgg gaaaagattt gcccgataaa acctgatttc
     7801 gatgcagtca tcgttcgtgc agccaaaaaa caaaagccta ttgtagcaaa tatcgcccgc
     7861 ctgctgaaaa acgccgcgcc gccgttttgc cgttccaatg caaaatgccg tctgaaactg
     7921 tgttcagacg gcattgcttt tctgaaaacc cgacgtgctt acaggctgta atacatttca
     7981 aattccagcg gatgcggcgc catgcggatg cggcggacat cttcctcttt aaaggcgatg
     8041 tagctgtcga tccagtcttt gctgaacacg ccgccgcgca gcaggaattc gtggtcggct
     8101 ttgagggcgg cgagtgcttc ttccaaagaa gcgcaaacgg tcgggacgag cgcgtcttct
     8161 tccggcggca ggtcgtagag gtttttatcg gcaggatcgc ccggatggat tttgttttga
     8221 ataccgtcca aaccggccat caacaaagcg gcaaatgcca agtatgggtt ggcggtcgga
     8281 tcggggaaac gcgcttcgat gcggcgcgcc ttgctgctgt tcacggacgg aatgcggatg
     8341 gaagcggaac ggtttttggc ggagtatgcc agtttggtcg gcgcttcaaa gtgcggcacg
     8401 aggcgtttgt aggagttggt ggacggattg gtaatcgcgt tcaaggcttt ggcgtgtttg
     8461 atgatgccgc cgatgtagta gagcgcggtg tcgctcaagc cggcatagcc gtcgcctgcg
     8521 aacaggtttt gaccgtcttt ccaaatggat tggtgaacgt gcataccgct gccgttgtcg
     8581 cccataatcg gtttgggcat aaaggtggcg gttttgccga agttgtgggc aacgttttga
     8641 atcacatatt tcatgtcttg ggtttggtcg gcgcgtttga ccaaggtggc gaagcgcgtg
     8701 ccgatttcca tttggctgcc ggtgccgact tcgctgtggt ggacttcgac ttcgatgccg
     8761 agttcttcca aaatgttcac catggcggag cgcaaatctt ggccgcaatc aatcggcgcg
     8821 acgggcgcgt aaccgccttt aacggcgggg cggtggccgg tgttttgacc gtccatatgc
     8881 agaccgcttg cccatgcgcc gctttcggac gtaatttcgt aacgggtttt gtgcatatcg
     8941 gtttcaaatt ctacgccgtc aaaaacgaag aattcaggct cagggccgaa ataggcggtg
     9001 tcgccgatac cggaagattt caaataggct tcggcgcggc gtgcgataga gcgggggtcg
     9061 cggtcatagc cttggccgtc agccgggtcg ataacgtcgc aggtaaagac aacggtggta
     9121 tcgtcgtaga aaggatcgac gaaggctgta gacgcatcgg gacgcaactg catatctgaa
     9181 gcctgaatac ctttccaacc gccgatggac gaaccgtcaa acgcctgtcc gttttcaaac
     9241 cactcttcgg ggtcttccaa cacgatgcgc gcaggcacgg taaagtggtg ctgcttgcct
     9301 ttggtatcgg taaagcgcaa atcgacaaag cgggcttcgc tttcttcaat caattttacg
     9361 gcgtttttaa tagacatttt tcagctcctg gaaaagggaa atagcaaaat atgcggaacg
     9421 cggcagatta gctcaaagcc agcatgattc taccacgtcc atgccgtctg aaactggccc
     9481 tgcgtcaaat tccaagctgt aaaatatagt ttcagacggc aatattgtca acaatatgct
     9541 tcggtttgcc cgttcccaag cagaatgccc gcccttttat ccgaatgccg acaaaacaga
     9601 tgccgtctga aagcagttca gacggcatct gttgtttggt ttgaaaacgg tcagcgagga
     9661 tagccgttgc agacaaattt gtactgagcc tctccaagcg taccgggaat aacataattc
     9721 cattcggcat aggatggtgt ataagaagcg atagagtttc ccgatttgga ataccacgtt
     9781 gccgtcaata tcctaaaacg tttaccactg caatccattt cttccaacgt tttagctgta
     9841 ttaaaaaatt ttccattgcc atacttttgc ggcttggcga accttgtttc aaaccatgca
     9901 ctgttgtccg tacggctgtc tgcatcacca aacgtggcag tattactatc taccaatacc
     9961 caatccgccg ccaccgccgg cgttgccaac cccaatgcaa atactgccaa gcacaacatc
    10021 tttttcatgt ccgaccctct cgtccttgtc aacaaaataa agttaaattg taaaaaaaac
    10081 gacttttttc aagagaaaaa tattccgtca tgctatctga caaccgtttc agacggcatc
    10141 gtttccatct gtacgccgcc gttataaaat agccttcccg tcttttggaa cacgaccatg
    10201 accgccctcc cccgttactc cgttttcggc aaccccgttg cacacagtaa atcgccgcaa
    10261 atccatcagc aatttgccct tcaggaaggc gttgacattg aatacgaacg catttgcgcc
    10321 gacatcggcg gtttcgcgca ggcggtttcg acattttttg gaacaggcgg ttgcggggca
    10381 aacgttaccg taccgttcaa acaggaagcg tttcatctgg cggacgagca ttccgaccgc
    10441 gctttggcag caggtgcggt caatacgctg attctgttga aaaacggcaa gctgcgtggc
    10501 gacaataccg acggtatcgg tttggccaac gacatcacgc aggtcaaaaa cattgccatc
    10561 gagggcaaaa ccattttgct tttgggcgca ggcggcgcgg tgcgcggcgt gattccggtt
    10621 ttgaaagaac accgccctgc ccgtatcgtc attgccaacc gtacccgcgc caaagccgag
    10681 gaattggcgc agcttttcgg cattgaagcc gtcccgatgg cggatgtgaa cggcggtttt
    10741 gatatcatca tcaacggcac gtccggcggt ctaaacggtc agattcccga tattccgccc
    10801 gatatttttc aaaactgcgc gcttgcctac gatatggtgt acggctgcgc ggcaaaaccg
    10861 tttttagatt ttgcacgaca atcgggtgcg aaaaaaactg ccgacggact gggtatgcta
    10921 gtcggtcaag cggcggcttc ctacgccctc tggcgcggat ttacgcccga tatccgccct
    10981 gttatcgaat acatgaaagc catgtaatat gttccgcatc gtcaaatggc tgattgccct
    11041 gcccgtcggc atctttatct ttttcaatgc ctatgtgtac ggcaacatca ttacctaccg
    11101 cgccgtcgcg ccccatcgga ctgcctttat gtcgatgcgg atgaagcagt ttgaacagga
    11161 aggtcgcgat gtcgcactgg attaccgctg ggtgccctac aaccgcatct cggtcaacct
    11221 gaaaaaagcc ctgattgctt ccgaagatgc ccgtttcgcc ggacacggcg gcttcgattg
    11281 gggtggcatt caaaacgcca tcaggcgcaa ccggaacagc ggcaaagtga aggcgggcgg
    11341 ttcgaccatc agccagcagc ttgccaaaaa cctgttttta aacgaaagcc gcagctatat
    11401 ccgcaaaggc gaagaagcgg cgattaccgc gatgatggaa gccgttaccg acaaagacag
    11461 gattttcgaa ctgtatctga attcgattga atggcactac ggggtgtttg gcgcggaagc
    11521 cgcgtcccgg tatttttatc aaatacccgc cgccaaactg agcaaacagc aggcggcgaa
    11581 actgacggca cgcgtccccg ccccgctcta ctacgccgac catccgaaaa gcaaacggct
    11641 gcgcaacaaa accaatatcg tgctcagacg catgggttcg gcagagttgc ctgaaagcga
    11701 cacggactga gtgccgccgc aatataaaat gccgtctgaa atatcattca gacggcatat
    11761 gctttctgcc tttaccctct tctccaaaac gcaacggggc ggtcattatc atttttgccc
    11821 ttctacataa cggtcagaca caaaacgtgt ccgacacgga caacttccca aaaacggcag
    11881 acaaaaaaat aaccgcttat cagcggtttt ttggtggcca gaggcggaat cgaaccgccg
    11941 acacacggat tttcaatccg ttgctctacc aactgagcta tctagccttt atctgtctcg
    12001 ttaagagatg cgtattaaac caaatttcgg gacaacacgc aagcggattt gtcgatattt
    12061 tttatccaac cgactaagtc gatgttttaa gtttaaatta atttcacaga aaaatagtgc
    12121 aaatcggttt tttgccgttt gttcggcgat attctgcttt tcagacggca aaacgcttca
    12181 atatttgaag ttctcgccca aatagacggc gcgcacctgt tcgtttccga ccaaatcatc
    12241 aggttttccc gatgccaaca ccgtgccgtc tgaaataatg taggcccgat cgcagatgct
    12301 gagggtttcg cgtacgttgt ggtcggtaat taatacgccg ataccgcgcg acttgagaaa
    12361 tccgatgatt ttttggatgt cgatgacggc gatgggatcg acgccggcaa aaggctcgtc
    12421 caacaaaata aaatgcggtt tcatggcaag tacgcgggcg atttcgacgc gtcggcgttc
    12481 gccgcccgac aaagatgggg caggattatt gcgcaaacgc tcgatattca ggtcggcaag
    12541 cagtttttca acttcgccgt cgatgcggct tttgtctttg gtactgattt ctaaaatggc
    12601 gcggatgttt tgttcgacgg tcattttacg gaaaatagag gcttcctgcg gtaggtagcc
    12661 gacacccagg cgggcgcgtt cgtgtatggg caggtgacgc aattcctgcc cgtccagcat
    12721 cacgctgccc gcatcggcgg caatgagtcc aacaatcatg tagaagctgg tggttttacc
    12781 cgcaccgttg ggcccgagca ggccgacgac ttccccgctt tcgatttcaa gggagaagct
    12841 tttaacgact tggcgttttt taaaactctt ttgcaggttt tgaacaacaa ggcggctgac
    12901 gtttgcgctc ataaatatct cttcaatatc ttcggtcggc atatgccgtc ggaaccgggt
    12961 ttatgtttca gacggcattt tgggattatt cggatttttg cgtactcgaa ggctggatga
    13021 tgacgctgac cctgccggat ttggaagcgg attttgcgcc ggattttgta ctgccgctga
    13081 tggtatagac ttcggttttg gtgttgtatg taatcaccgc gccttcggcg acatcgccgc
    13141 cgcgctgtac tttggcatta ccggttaaga ctacggtgct gcctgcagac gaataagcaa
    13201 cgttgtttgc ctgaccgcgc accgtccctt tgcccccgtc caacgtttgg ctgaagcgta
    13261 cgggcgagcc gtcggccttc ataaactgtt cgcctttgtc attacgcgtt acgttgacgc
    13321 gggcggcgga aatattgagc gtaccctgtc tgatgacaac gtttccgcta aatgtagtgc
    13381 tttggttggc ttgattgaga gaaccttggt cggcctcaat ctgaataggc tgcctgctgt
    13441 cgctttgaag ggcaaaagcg ggggacgccg aaaaaaatgc tattaaaaca aatagcttac
    13501 atatcttttg tatcataaat cgtggctttc actttagatg agaagttcaa catgcctgtt
    13561 ttgtggtcgt aagtcatgcc gcccgcctga ccgtgcgatg caccatattg gaaactgaca
    13621 ggcgtatcgg tttgggcata ttgagattcg gtatcgacgt gcagcttttc ggcttcaact
    13681 ttacccgcct gccgtttgcc gtcggcggtt ttggtcagca caacgttgtt tttaaaaaga
    13741 acctgtttgt tttcggtatg gtaaacggct tcatcgctgc cgacttcgta caacaacctg
    13801 ccttcttgga agaacacgag atgcggtgaa tcgaaatgaa tgtcgctgct ttcgggaaac
    13861 tgtttcgcgc ctttcgcgct caaatgttct tttaagtatc cctgttcgtc aaaccgcctg
    13921 ccatccaagc cgtccattgt gtattgcggt tcgtcgggat tgagcctgac ttcttcaatc
    13981 tcgacttcgc tgatgcgtcc caaccatgcc gacagaccgc ccaaggcaac cgccaatatc
    14041 aatgggaacg caattccgta ccgccatctt attttcattt gatgtactcg ttcaaagccg
    14101 cgcccaaagt cccttgcgcc tgcataatca ggtcgcacac ttcgcgcacc gcgcccgcac
    14161 cgccggggcg tttggcaata taggcggcgt gttgcagcgt aaaccaatgc gcgtcgggaa
    14221 cggcaaccgg caatccgcaa cgcaccatca ccggcaaatc gaccacgtcg tcgccgacaa
    14281 aggcacactc ggcttcttcc acgccgacct gagcgcgcag ctcttcatac gcggcgcgtt
    14341 tgtccgaaat gcctttgaag tagtaattta tgcccaactg tttgacgcgg atgccgacgg
    14401 agggcgcgtc ccgacctgtg ataatcgcag tctgcacgcc gcttgcctga agcattttca
    14461 gaccgtgtcc gtccagtgtg tgaaacgatt tgatttcttc gccgttatcg cggataaaga
    14521 tgcgcccgtc ggtcaaaaca ccgtccacat ccaggatcag cagtttgatt ttggcggcgc
    14581 gcgcctgtaa ttcaggagaa attgcctgca tgggatttcc ttgttttcag acggcatatc
    14641 ttaccacaaa taccgtctga aacggctgca acgccttata caatccgcgc cgccaaaagg
    14701 tcgtgcatat tcagtgcgcc gatcagcacg ccatctgcat cggtaaccag aagcccgttc
    14761 acatggtttg cctgcatgac tttcagggct tcggtggcga ggcgttcggc ggcgatggtt
    14821 ttagggtgtg tatgcatgac ttcgtctatc gaaagaccgg taaaattgtc gcattcttga
    14881 aacaggcggc gcaaatcgcc gtcggtgaat acgcctttca gacggccttg cccgtccgtt
    14941 accgccagca tccccagccc tttttcactc atgctgacga tggcttcttt caagggcgtg
    15001 ccgagtcgga cggcaggcag gccgccgcct ttgtgcataa tgtcggcaac gcgcaaaagt
    15061 aggcgtttgc cgaggctgcc ggcagggtgg ctcaaggcga agtcgtcggg cgtgaacgcg
    15121 cgcgcgcgca acagaacgac cgccaacgca tcgcccaaag ccatgacggc ggtggtgctg
    15181 gtggtcgggg caagccccag cgggcaggct tcttgcgaaa ccgatgcggt gatatggatg
    15241 tcggcatggc gcgccatggt tgaatcgggg cgggcggtga tgcagacaag cgtgatgttt
    15301 ttgcgtttga gtgcggggat gatggcggcg atttcgtcgc tttcgccgga attggaaatc
    15361 gcgacgacca cgtcgttgtc cacaatcata cccaaatcgc cgtgtgccgc ttccgcaggg
    15421 tggacgaaaa acgcaggcgt gccggtcgag gccatagttg ccgccatttt gcgcccgata
    15481 tgtcccgact tgcccatgcc cgtgataacg accctgcctt tgcagtgcaa caacgcgtcc
    15541 gccgcaaggg cgaagttttc gtccaaatcc gccgcaattt cgcgcaagcc ttccgcttcg
    15601 gtgtgcaaca cttcgcgcgc ccaatcgaga tattttccgt tttctgccat tgccatccgc
    15661 ctttgctgta acaaaactac ccaaaatttc cgaatcgggg tagcataagc attcaaaccc
    15721 cgccatccat ccaaaggaat cagaaatgac tattttatcg gacgttaaag cattaggaca
    15781 acaaatctgg ctggacaacc tttcccgctc gctggtgcaa agcggcgaat tggcgcaaat
    15841 gttgaaacaa ggcgtgtgcg gcgtaacttc caatcccgcc attttccaaa aagccttcgc
    15901 cggcgacgcg ctttatgccg acgaggttgc cgccctcaag cagcaagacc tcagccccaa
    15961 acaacgctac gaaacaatgg cggttgccga tgtgcgtgcc gcctgcggca tctgtcttgc
    16021 cgaacacgaa tccaccggcg gcaaaaccgg tttcgtcagc ctcgaagttt cgcccgaatt
    16081 gtccaaagac gcgcaaggca cggtagaaga agcgcgccgc ctctacgccg ccatcgggtg
    16141 caaaaacgcg atgattaaag tgcctgccac cgacgcaggc atcgatgcgc tcgaaaccct
    16201 cgtttcagac ggcatcagcg tgaacctgac cctgctgttc tcacgcgtcc aaaccctcaa
    16261 agcctacgcc gcctacgcgc gcggcattgc caaacgcttg gcagccggac aaagcgttgc
    16321 ccatatccac gtcgtcgcca gcttcttcat ctcccgtgtg gacggcgcgc tggacacaac
    16381 gctgcccgac cacctcaaag gcaaaatcgc catcgccctt gccaaagccg cctatcaaga
    16441 ttgggaacaa tatttcacca cccccgaatt tgccgcactg gaagcccaag gcgcaaaccg
    16501 cgtgcagctt ttatgggcat ctaccggcgt gaaaaaccca gcctatcccg acacgctcta
    16561 cgttgacaac ctgatcggcg cgcacaccgt caacaccgtt cccgatgcca cgctcaaagc
    16621 ctttatcgac cacggcacag ccaaagccac gctgaccgaa ggcgtagaag aagcacaagc
    16681 gcagcttgcc gaaaccgccg cgctcggcat agacgtagaa accctcgcca accgcttgca
    16741 ggaagacggt ttgaaacaat ttgaagaagc gtttgaaaaa ctgctcgcac cgttggttta
    16801 aacttttcat ccccgatgcc gtctgaaagg ctttcagacg gcatcgccct tttgaccgga
    16861 cacagtgcgg catatcaagg attcgcccta aaaatattaa tcaacaagaa gttaatgcac
    16921 gacattaccc actaacacaa aaggaaatag aatcatggca aaagcactcg aaatcatttc
    16981 acccaatgaa atttattcag atctgatttt taaggatccg gtacctcccc atactatata
    17041 tagagaattg atgaaactgg ttagtagaaa tgcaagtaat gagagaatcg cagatagagc
    17101 cttcgatttt ttctcgtccg gccttatgga tggttcagct accgaagagc aatataatgc
    17161 tctatatgat ttaaccttgc ttgaagagcc cgggatggaa ctgaataaag atgagataat
    17221 ggctttgatt catagtctta aataaagggg ctgtaccgga tatgtacaca ggacgtttcg
    17281 cccccagccc gaccgggctg ctccacatcg gctcgctgct gaccgccgcc gcttcctatg
    17341 ccgatgcacg ctcaaacggc ggcaaatggc tggtccgcat ggaagacctc gatccgccgc
    17401 gcgaaatgcc gggggcggca agccatatcc tgcacacgct tgaggcattc ggattcgagt
    17461 gggacggaga agtcgcctat cagagccacc gttacgccct gtatgaagaa accctatgcc
    17521 gtctgcaaac cgccggactg gtctatccct gccattgcag ccgcaaagac tggcaggccg
    17581 gggcaaggcg gggcgcagac ggattcgtct ataacggacg ttgccgccac ccctgccaac
    17641 gccctgcacc gcaaggcaaa cagccggcgt ggcgcatccg cgtccccgac cgcattatcg
    17701 gtttttcaga cggcatcgtc ggcggttacg cccaaaacct cgcccgcgac atcggcgatt
    17761 tcgtcctgct tcgtgcagac ggttactggg cataccaact cgccgtcgtt gccgacgatg
    17821 ccgaacaggg cgttacccac atcgtccgcg gacaagacct gcttgtttct acgccgcgcc
    17881 aaatctattt gcagcagtgt ttggacgttc cgacaccgca atatgcccac ctgccgctgc
    17941 tgaccaacgc acaagggcag aaatggtcga aacagacgct cgcacccgca ttggatttaa
    18001 accgccgcga acaactcctc cgccaagtgt tccgttacct caacctgccc gaagcaccgg
    18061 aagccgaccg ccctgccgaa ctgctcgact gggcggtggc acactgggat atgggcaaag
    18121 tgccgaaaca cgccattacc accccctaaa caccgcccga caaacgcaca aatgccgtct
    18181 gaagccttca gacggcattc cacacataca gccccgccct attcgcgcat atttgcccgt
    18241 tcgacttctt cccacgcttc gagaatcagg ctgccgtcgt tgtctttcaa gagcgttgcg
    18301 tcggaaagca tacgccgcca agtccgcgca cctttcaaac cgtgcgtcag cccgaggctg
    18361 tggcggacga tgtggcgcaa tattgtgccg cgtccggctt ggatttgggt ttggctgtat
    18421 gtgtagagac gctgcaccaa atcggcgtat tcaatcgggc tgcgggtatc gccgtaaaac
    18481 agcctgtccc attcgcgcat caccatcggg ttgtggtacg cctcgcgccc gaccatcacg
    18541 ccgtcaacgt gttgcaggtg tccggcgatt gcttcgttgg tggtgatgcc gccgttgatg
    18601 atgatttcca gcccgggaaa ctcctgcttg aggcggtaaa cgtaatcgta tttcaacggg
    18661 ggaacgtcgc ggttttcttt gggggaaaga ccgtccagcc aagcgttgcg ggcgtggacg
    18721 atgaaggttt tgcaggcggt tttgtcgcgc agcgtgccga cgaaatcggc aacggtttgg
    18781 tattcggtct gcctgtccac accgatgcgg tgtttgacgg taacgggaat gccgaccgcg
    18841 tcctgcatgg cgttgaggca gtcggcaacc agcccgactt cgttcatcag acacgcgccg
    18901 aacgagcctt tctgcacgcg cggactgggg cagccgcagt tgaggttgac ctcgttgtaa
    18961 ccgtatgcct cggcggcttt ggcggctttc gccaaatcgg acggatcgct gccgcccagt
    19021 tgcagggcga cgggctgctc gccttcgttg aacatcaaaa agcggtcttt gtcgccgtaa
    19081 acaatcgcac cggcattgac catttcgctg tacagccaag tatttcgggt aatctggcgg
    19141 gcaaggtaac ggtagtgcct gtccgtccag tcgagcatcg gggcgacgga aaggcggcgc
    19201 gggggaaggg tgtgtgtatt gtcgttcatt gtcgatattt caccattgcg gattcaattt
    19261 caatccggaa caaccttcat ttttggggaa atccgcgtca tgaaagagtc cagcagtatg
    19321 gttaccggcc cgtcgttgca gagcgacacc tgcatatgcg tgcggaaacg ccctgtttcg
    19381 acatgaattc cgtatcctcg caacagttct gccgttcgca ggtaaagctg ctgcgcctgt
    19441 tctgcaggtg cggcttggga aaacgaaggc cgccgcccgc ttgccgcatc ggcataaagc
    19501 gtaaactgcg acaccagcag cacggatccc ccgacatcct tcaaagacag gttcaacttg
    19561 cccgcttcgt cttcaaacac gcgcaaatgg gcgattttgt cggcgatata gcgtgcatct
    19621 ttttctgtgt cgctatgcgt tacaccgagt agcacgacga atccgccgtc gattttgccg
    19681 caggtttccg tgccggcttc ggacacgaca tccacctttg cacctaccgt tttctgtatg
    19741 accgcacgca taagccgtcc tccttacctg aacgtgcaat gccgtctgaa aaggcttcag
    19801 acggcatcgg tattggaaat gccattacgg tttgacaccg ttttcttcca aaatggcatc
    19861 gattttcaag cgggcttttt cctgtctcaa accttgttcc aactggtttc tgaccaactc
    19921 gaaaggctgc gcgtcggggt ttttcccgac ctcgctgagt ttgaacagat aatagcgttc
    19981 gcccaatttg accggatcgc gggtaacgtc gccccgattc atcgctgcaa actgcgaagc
    20041 cagcggctcg ggaagctgct gcgccataat gaaaccgtca aaagcctgct cgtcgttcgg
    20101 ataacgcttc atcagccctt caaaagacag ccctttgagc aggagctgct gcgcctgacg
    20161 cgcctcctct tcggttgcga agctgacctg ctgcaatttg atcatgcgga tttgccgctc
    20221 ataaaactga cgcagtgcgc tttcggaaac cgtttccgaa cgttccagaa aacggacgta
    20281 ctcctcggca taaaaagacg cttcggcgat tttaaagcgg ttttggacat ccttatcctt
    20341 atccaaacct tccttcaatg ccctgttttt caaaacttcc aaagtttgca gccgacggac
    20401 ggcatcgttt cggattgcct gcccgtccgg tttttgggac tgctccgcat gccggtctgc
    20461 ctgctgcatg atctgcgcca ccagcgtatc caccaaagcc gggtcgattt cgggtgcttt
    20521 ggctgccgca aaacctgcca acattgcagc aataactgcg gcagcggttt ttttctgttt
    20581 catggtattc cctgtcctgc gcggcggcaa aacgcctgtc tgaacgtctt gccgccatat
    20641 cccgttttca gaattatttt gcaggtttga tgtttgcctt gcccaacagt gcaccgacgg
    20701 cacggtcaat ccgttccgcc tgaaggttgc ccgcaatctg tcctttcatt tcatcaaaag
    20761 aaggcacttt tacctcgcgg ctgtcgttga cataataaac gccgtagaaa tcgccgtttt
    20821 tcagcggcgt tgccgtaaat tcgccttttt tcaagtcctt aattgcctga taaagcggcg
    20881 gaacaccctg ttccaaatct ttcagcggca catatccgac cggcgcaccg gtctgtttgg
    20941 tacggtcgtt gagggaatat tgtttcaaga cggcatcgaa accttttttc gccttcaagt
    21001 cggcaaccgc tttttttgca ttttcttcct tgtcggtcag gatttcgccc aactggactt
    21061 cctgcgtacc tttgtaaaaa ccgctgatat tgtcatatgc ggcttttact tcctgctcgg
    21121 aaaccggttg ggttttggcg atatgcaatg cgtatgcctc gccgttcaag ccatatttta
    21181 ccgcctgcca aacggttttg aaggacggtt tcttgtcgtc gcccgacttt ttcgcttcgg
    21241 cacgcaattt ggcaagcgca tttttaaact ctgccgaccg gtcgagtttc aggcgtttca
    21301 cttcctgtgc gaccacggta ttgaccactt cgttttccag cagggattgg cgcagttgcg
    21361 gcgtgtcttc ggcacggctg ttttccgcac ggaatgcggc aacctgcgca tcgatgacgg
    21421 aactgtcgat tttctgaccg ttgacggttg ccagcgtttg ggcaaacagg ctgccggaac
    21481 aggcaagcag tgcaacggaa gtcaggattt ttgctttcat aatcttctct ctttaaattt
    21541 taaattgaaa tgttcgggca gacttcattc agtccgccgt atggaaatac tcgtcagggg
    21601 tagccgcccg gatgccgagc gcgtgaatca tgccgcctga aaacaaatct ttgagcagcg
    21661 atttgaccgt tttctggcgg ttcaggcggc ttacgccttc aaaacggccg ctaacgacca
    21721 aaacggcata atgtccgccg ccggtattgc ccgcgtgtcc tttgtgcaga tggctctcat
    21781 cgccgatttc caacaccagc ggatcgagcg tctgcaggcg ttcgcggatc aaatcgacgg
    21841 ccggcattat ttgaacaccg ctttaaacgg cttgatcagc acttcgctgt acacgcctgc
    21901 atgaacatag ggatcgtctt ccgcccacgc ctctgccgca tccaaagact cgaactgcgc
    21961 cacaatcaaa ctgcccgaaa cgcggttgga gtcctccggc aaaggattcg ggcctgccgt
    22021 caacagccgg ccttccgact tcagcgtctc cagccgtttg aggtgttcgg gacgtgccgc
    22081 catacgcgct tcatacacat tctccccgtc tgttgccagc aacataaaat attccacagt
    22141 caatcctcct ttttcagaca ggtactcaga taaataccct gaataataaa gaaaacaagc
    22201 atcagtgcag tcgagccgaa cattttatag ttgacccatt gcgactcgaa ccgggtaaac
    22261 acaaaccagt tggcgatacc cataaaaatc aggaaaccga cccacatata cgtcaatttc
    22321 gcccatacgg catccggaag ctgaatctcc ctgccgatac tcgccttcaa gccgttttta
    22381 ccggcgaggt ggctgcccca caggaacagc gcgcccagcc aaaacaaaac gctcggcttc
    22441 cacataatga agcggctgtc gcccaaaaca atggttgcgc cgccgaatac cacaatcagc
    22501 accaatccga cccactgcat cgtatccagc tttttatatt tccaatacag aaaagccgcc
    22561 tgaaccacac cggcaaccaa ggcaaccgcc gttgcggcaa tcatgttttt ggtaacggta
    22621 taggtggcga aaaacaggat gacggacaaa aggtcgctga cgaatttcat atccaaatga
    22681 gaaagcaaat caaataaacg ctaatcatac aggcagaacc ctgtttttgc acctgcaaaa
    22741 ccaacaaatt ttgttttgat tgatgcaaat atgcacagat gtttttgaaa aaagatggag
    22801 atatgtcata atttgtaaaa acggcgaatg attgccgttt aaaatgtggc ggcggtcggt
    22861 acattcacat attaaatgcc cgccggccga ctgccccgcc gatgcccgat gatgacgaaa
    22921 tttacagact gtacgcggtc aaaccgtatt cagccgccaa cccacagggg atacatcttg
    22981 aaaaacaaca gacaaatcaa actgattgcc gcctccgtcg cagttgccgc atcctttcag
    23041 gcacatgctg gactgggcgg actgaatatc cagtccaacc ttgacgaacc cttttccggc
    23101 agcattaccg taaccggcga agaagccaaa gccctgctag gcggcggcag cgttaccgtt
    23161 tccgaaaaag gcctgaccgc caaagtccac aagttgggcg acaaagccgt cattgccgtt
    23221 tcttccgaac aggcagtccg cgatcccgtc ctggtgttcc gcatcggcgc aggcgcacag
    23281 gtacgcgaat acaccgccat cctcgatcct gtcggctact cgcccaaaac caaatctgca
    23341 ctttcagacg gcaagacaca ccgcaaaacc gctccgacag cagagtccca agaaaatcaa
    23401 aacgccaaag ccctccgcaa aaccgataaa aaagacagcg cgaacgcagc cgtcaaaccg
    23461 gcatacaacg gcaaaaccca taccgtccgc aaaggcgaaa cggtcaaaca gattgccgcc
    23521 gccatccgcc cgaaacacct gacgctcgaa caggttgccg atgcgctgct gaaggcaaac
    23581 ccaaatgttt ccgcacacgg cagactgcgt gcgggcagcg tgcttcacat tccgaatctg
    23641 aacaggatca aagcggaaca acccaaaccg caaacggcga aacccaaagc cgaaaccgca
    23701 tccatgccgt ccgaaccgtc caaacaggca acggtagaga aaccggttga aaaacctgaa
    23761 gcaaaagttg ccgcgcccga agcaaaagcg gaaaaaccgg ccgttcgacc cgaacctgta
    23821 cccgctgcaa atactgccgc atcggaaacc gctgccgaat ccgcccccca agaagccgcc
    23881 gcttctgcca tcgacacgcc gaccgacgaa accggtaacg ccgtttccga acctgtcgaa
    23941 caggtttctg ccgaagaaga aaccgaaagc ggactgtttg acggtctgtt cggcggttcg
    24001 tacaccttgc tgcttgccgg cggaggcgcg gcattaatcg ccctgctgct gcttttgcgc
    24061 cttgcccaat ccaaacgcgc gcgccgtacc gaagaatccg tccctgagga agagcctgac
    24121 cttgacgacg cggcagacga cggcatagaa atcacctttg ccgaagtcga aactccggca
    24181 acgcccgaac ccgctccgaa aaacgatgta aacgacacac ttgccttaga tggggaatct
    24241 gaagaagagt tatcggcaaa acaaacgttc gatgtcgaaa ccgatacgcc ttccaaccgc
    24301 atcgacttgg atttcgacag cctggcagcc gcgcaaaacg gcattttatc cggcgcactt
    24361 acgcaggatg aagaaaccca aaaacgcgcg gatgccgatt ggaacgccat cgaatccaca
    24421 gacagcgtgt acgagcccga gaccttcaac ccgtacaacc ctgtcgaaat cgtcatcgac
    24481 acgcccgaac cggaatctgt cgcccaaact gccgaaaaca aaccggaaac cgtcgatacc
    24541 gatttctccg acaacctgcc ctcaaacaac catatcggca cagaagaaac agcttccgca
    24601 aaacctgcct caccctccgg actggcaggc ttcctgaagg cttcctcgcc cgaaaccatc
    24661 ttggaaaaaa cagttgccga agtccaaaca ccggaagagt tgcacgattt cctgaaagtg
    24721 tacgaaaccg atgccgtcgc ggaaactgcg cctgaaacgc ccgatttcaa cgccgccgca
    24781 gacgatttgt ccgcattgct tcaacctgcc gaagcaccgt ccgttgagga aaatataacg
    24841 gaaaccgttg ccgaaacacc cgacttcaac gccaccgcag acgatttgtc cgcattactt
    24901 caaccttcta aagtacctgc cgttgaggaa aatgcagcgg aaaccgttgc cgatgatttg
    24961 tccgcactgt tgcaacctgc tgaagcaccg gccgttgagg aaaatgtaac ggaaaccgtt
    25021 gccgaaacac ccgatttcaa cgccaccgca gacgatttgt ccgcattact tcaaccttct
    25081 gaagcacctg ccgttgagga aaatgcagcg gaaaccgttg ccgatgattt gtccgcactg
    25141 ttgcaacctg ctgaagcacc ggccgttgag gaaaatgcag cggaaatcac tttggaaacg
    25201 cctgattcca acacctctga ggcagacgct ttgcccgact tcctgaaaga cggcgaggag
    25261 gaaacggtag attggagcat ctacctctcg gaagaaaata tcccaaataa tgcagatacc
    25321 agtttccctt cggaatctgt aggttctgac gcgccttccg aagcgaaata cgaccttgcc
    25381 gaaatgtatc tcgaaatcgg cgaccgcgat gccgctgccg agacagtgca gaaattgctg
    25441 gaagaagcgg aaggcgacgt actcaaacgt gcccaagcat tggcgcagga attgggtatt
    25501 tgattcccaa ctgccctttc gcagatcaag gatgccgttt cagacggcat cttttttgcc
    25561 ttatcggtgt aacggataaa gtttgaaccg gcacaggctc aaacagcagg tcgacggcaa
    25621 caaaatgccg tctgaaaccc ctaaaggctt cagacggcat tggcggcggc ggtatcaggc
    25681 agtttgatag gcaaccgaat cgtcgccgct tttcttttga atgaactcga ttttgtatcc
    25741 gtcggggtct tcgacgaagg ctatcacggt tgtgccgtgt ttcatcgggc cggcttcgcg
    25801 gacgacgttt ccgccctgcc gcttcacacg ttcgcaggct tcgtaggcat cgtccacttc
    25861 aaccgcgatg tgtccgtagg cgttgcccaa gtcgtatcgt tccgtatccc agttgtgcgt
    25921 cagttccaaa accgtgctgt cggtttcatc gccgtaaccg acgaaggcaa gggtaaatct
    25981 gccttcggga taatcttttc ggcggagcag tttcataccc aaaacgtttt ggtagaaatc
    26041 gagggatttt tcgagattgc ccacgcggag catagtatgg agtaagcgca ttttttgtgt
    26101 tcctttcggt ggtggttaaa cttcgatttt attcggggta aacgtctgcc atttgttgca
    26161 ggcggggcag tgccagaaaa agacttggga tttgaagtgg cagttgcggc aacggtacat
    26221 cacgctgcgc tgtagctgcc gtccgataac cgaacgcatc atgtcggcat cggctttcca
    26281 agccggattc atatcgctga gtttcaaacc gagcaggcgg tacacgccgt taaggtcggg
    26341 cttgcggcgg acaagctcga cggcggtttg cgcggcttct ttctcgcact taagcagcag
    26401 ggatttctcg tacacgacat tgatcaggtc aagttcggga aacgtctgca tatatcctgt
    26461 cagacggttc aagccttctt caggttttcc ctgcgcggca taggcttcgt aaagcttctc
    26521 gccgaccatg ctcaagtatg catggttttg ctgctcgatg gcggcatagg cttcgacggc
    26581 ggcagggaaa ttgccttgtc ggtgttcgat gtcgcccaaa atcatgttgg cgcgggtgca
    26641 ttttttgttg gcttcgagtg ccttgccgac attgaaacgc gcgacatcga aattggactt
    26701 gaacagcgcg gcttgggcaa gttcgcaata aaactgggcg atttcaaact gataggtctg
    26761 atcgtcatgg ctgagcagcc gggcggtttc aaccgctttt tcccaatccc tgtcctgttg
    26821 gtagatattg agcaggtgct gtctggcttc acgcgccatt ttaccgtctt gcagccccaa
    26881 aaaaatctgt tcggcacgat cgaccaaccc cgcactttgg tagttttgcg ccaattcaaa
    26941 caggacgcgc gcgcgctttt cgccgaccgt atcgggagaa tcgagcattg tccggtgtat
    27001 gttgatggct ttgtcgtttt cgccacgctg gcggtaaagt ttgccgaggg tgaggttcaa
    27061 atcatacgat tgcggccggc cgtcgacgac ttccgccaac tcccttgccg cgcgcccgct
    27121 gttgcggtcg accaaagcgt ccaagctttt ataaaatccc gaagggatgc tttttgcctg
    27181 cttcaatacg gttttcatat ccacgcgggc ggcaaaccag cccatcgcga agaagacggg
    27241 caaaaggata atcggcagca ggataatcca caattcgttg tccatatcgg ctttcttaag
    27301 gctgtttggt agattcgggc gcattttgcg ccggtggtgc ggtcagctcc ttccccgtca
    27361 aacgcgcatt tttctttact tcggcacgca acctgccgtt ctcgccacgt aacgacaaca
    27421 accgtccgaa caaggcaaac attccaaaaa taataccgac tacaaatgcg ccgaacaata
    27481 cgacaatcag cggcaaatcg aatttttgcc ccggcaggta ggagaaggta acggcatccg
    27541 tattaatgac ggcaagcagc aggaagaaca gcaggataat gattttgacg acggtataga
    27601 taagtttcat gtttgctccg taaataaaaa cgccgcccgg acagcttcgc actctgaacc
    27661 aagattgcgc tagtttaaac gatttgcacg gtttcggata ggatgcggca gcgtgttcgg
    27721 acgacatacg gagtatgcga ttgccgacaa ttttaccaaa tacaccgttc ctttccattt
    27781 gaaaaataac ggattggaca ccgcatcgac agaaaaaccc gccgcgcact tgtcaaaacc
    27841 ctgtttgcag gcgtatcttt acaatcttca aattcaaacc gttcattgaa acatatcaga
    27901 ataagaaagg ctttacatca tgagcagacc cgtacccgcc gtattcggca gcgtttttca
    27961 cagtcaaatg cccgtcctcg cctaccgcga aggcaaatgg cagccgaccg aatggcaatc
    28021 ttcccaagac ctctccctcg taccgggcgc gcacgccctg cactacggca gcgaatgttt
    28081 cgagggactg aaagccttcc gtcaggcaga cggtaaaatc gtactgttcc gtccgactgc
    28141 caatatcgcg cgtatgcggc aaagtgcgga cattttgcac ctgccccgcc ccgaaaccca
    28201 agcctatctc gatgctttgg tcgaactggt caaacgtgcc gccgacgaaa ttcccgatgc
    28261 gcctgccgcc ctgtacctgc gcccgacctt aatcggtacc gatcccgtta tcggcaaggc
    28321 cggttctcct tccgaaaccg ccctgctgta tattttggct tcccccgtcg gcgactattt
    28381 caaagtcggc tcgcccgtca aaattttagt ggaaaccgaa cacatccgct gcgccccgca
    28441 tatgggccgc gtcaaatgcg gcggcaacta cgcttccgcc atgcactggg tgctgaaggc
    28501 gaaagccgaa tacggcgcaa accaagtcct cttctgcccg aacggcgacg tgcaggaaac
    28561 cggcgcgtcc aactttatcc tgattaacgg cgatgaaatc attaccaaac cgctgaccga
    28621 cgagtttttg cacggcgtta cccgcgattc cgtgctgacg gttgccaaag atttgggcta
    28681 taccgtcagc gaacgcaatt tcacggttgc cgaactgaaa gctgcggtgg aaaacggtgc
    28741 ggaggcgatt ttgaccggta cggccgcggt catttctccg gtaacttctt tcgtgattga
    28801 cggcaaagaa atcgaagtga aaagccaaga acgcggctac gccatccgta aggcgattac
    28861 cgacatccag tatggtttgg cggaagacaa atacggctgg ctggttgaag tgtgctgatg
    28921 ctttaaataa aaaaatgccg tctgaaaccc gtttggcgtt tcagacggca ttttcgcgtt
    28981 cggaccattt ccgccgcacc tgcagcaagt cggcataaag gcaatcggtt aaaacaagcg
    29041 tccgcatttc ccatcccgcc tgccgcaagt cgggcatttc tctggaaata cgcttcagac
    29101 ggcaaaccgc cgcccgaaac cgatatgcgg cacggacggc gcacggattt gaaaacggcg
    29161 gattatccct cggtgctcaa ggcattaatg ctgtaaccgc cgtcaacgta agtgatttcg
    29221 ccggtaatgc cggacgacag gtcggacagc aggaaggcgg cggtattgcc gacttcttcg
    29281 atggtaacgt tgcggcgcag cggattgtgg gcggcgacgt gtcccaagag tttgccaaaa
    29341 tcggcgatgc cggaggcggc gagtgttttg atcggaccgg cggaaatgcc gttgcagcgg
    29401 atgccttctt tgcccaaaca ggcggcggta aagcggatgc ctgcctcaag gctagctttt
    29461 gccataccca tcacgttgta gttcggaatc gcgcgcaccg cgcccaagta gctcagggcg
    29521 acgatggcgg agtttctgcc gcgcatcatc ggacgggcgg cttttgccag cgcaggcagg
    29581 ctgtatgcgg aaatttcgtg tgcggtgttg aacgcttcgc ggctgatgct gtcgaggaaa
    29641 tcgccgctca aggcttcttt cggcgcgaag ccgatggaat gcaccaaacc gtccaagccg
    29701 tcccaatgct tgcccaagtc ggcgaacact tggttgattt cgtcgtcgct ggcgacatcg
    29761 cagcggaaca cgagttcgga atccaattcc gccgccattt tgcggacgcg ctcttccagt
    29821 ttgtccacaa cgtaggtaaa cgccagttcc gcgccttgtt cgcggcaggc tttggcgatg
    29881 ccgtaagcga tggaacgctc ggaaatcatg ccggtaatca gaattttttt gccttgcaga
    29941 aaacccattt tgtatccttc aaatagtgtt cggttcgttc taaaccgttg attatagcaa
    30001 attgtccctg tttctgtgtt ttcacgttgc agcatgcaaa cggcaatgcc gtccgaagcg
    30061 gatttcagac ggcatcggac gtttcaaata cggtttaagg catcagatgc cgcgcaacaa
    30121 ttcgttcacg ctggttttcg cacgggtttg cgcgtccacg cgtttgacga tgacggcgca
    30181 gtaaaggctg tggctgccgt ctttggaagg catactgccg gacacgacga ccgaacctgt
    30241 cggtacgcgg ccttggtaga tttcgccggt ggtgcggtcg aagattttgg tggattgacc
    30301 gatgaacacg cccatcgaaa tcacgctgcc ttcttcgaca atcacgccct caacgatttc
    30361 agaacgcgca ccgatgaagc agttgtcttc aatgatggtg ggggcggcct gcaggggttc
    30421 gagtacgccg ccgataccga cgccgccact caagtgcacg tttttaccga tttgcgcgca
    30481 agagccgaca gtcgcccaag tatcaaccat cgcgccttcg tcgacgtatg cgccgatgtt
    30541 gacgtaagac ggcatcagca cgacattttt cgccacaaag ctgccgcgtc gggcaaccgc
    30601 acccggaacg gcgcggaagc ctgcgttttt gaactcgtct tcagaccagt cggcaaactt
    30661 ggtcggcact ttgtcgaagt atttgttcac gccgtcgttg aggacttcgt tgtcttggat
    30721 gcggaaggac agcaacacgg cttttttcgc ccattcgttg actttccact cacccacgcc
    30781 caagcgttcg gcaacgcgca gtttgcctga atcgagttgg cggatggttt ccaacacggc
    30841 ttctttgact tcgggagtaa cggtggtcgg ggtaatgtcc gcgcggtttt caaaggcggt
    30901 ttcgataatg ttttgcaaag acataatgtt tccttatgtg agatgtttcg tgggacgctt
    30961 gccggtattt tcaagcagta tgttcatttg aaatgaatgc gctattgtgc cacgcaaacg
    31021 catattcgtc agcaatacac gggcggttgc cgaacattgg cgtttcggaa tcgggcgggc
    31081 ggcaggtatt gccttttccg tgcccgaatc aatacagaac ggcggcggca cttttatgcc
    31141 gcgccgccgc ctttcagacg gcattcgcgg taaaacgcca tcagcccttc ggtcgaggca
    31201 tcgtgtacgg acgtgccgcc ttccagttcg ccgatgatgg ttttcgccaa ctgtttgccg
    31261 tactcaaccc cccactgatc gaaggggttg acgttccata tcgcgccttg gacgaaggtt
    31321 ttgtgttcgt aagccgccat cagcataccc agattgtagg gcgtgaggcg gtcgagcagg
    31381 atgctgttgc tggggcggtt gccggggaat tctttgtgcg gcgcgaggcg ttcgcgttcc
    31441 gcttcgggca aatctgccag ttcggcgcgt gcttcgtcca aggttttgcc cttcatcagg
    31501 gcttccgctt gggcaaaggc gttggcaacg gtaaaacggc tgcgtccgtc ctccacgccc
    31561 tgcgccgtca tcgggacgat aaagtcgcag ggaatcaggc gcgtgccttg atgaagcagt
    31621 tggaaatagg cgtgctggca gttgacccct tcaccgccga acacgatgcc gcccgttttg
    31681 cacacggcgg gactgccgtc tgaagcgcgg cttttgccca aactctccat atcgagctgg
    31741 ttcagccacg ccggcagcag gcgcaggttg tggctgtacg gaacggcagt ctgcccatcc
    31801 gcgtgctgga aattgttata ccacacggca atcagtgcca tcagcacggg aatgttgcgg
    31861 cgcagcggcg tgtgaaaaaa atggctgtcc atcgcgtgcg cccccgccaa caactcgcgg
    31921 aaacgcgccc cgccgaccgc aaccatcacg ggcaaaccga cgggcgacca gacggaatag
    31981 cgtccgccca cccagtcgta catcgcaaac acgcgttccg ccgcaatgcc gaaactttgc
    32041 gccgcttccg tatcggcaga caccgcgcaa aaatggcgcg ccgtttcgga ttccgagaac
    32101 cccgcaccgc gataccacgc cttgaccgcc tgtgcattga gcagggtttc tggtgttttg
    32161 aaggacttgc tggcaacgca aaacactgtc gtttcggggt tcagacggca taaaaccgca
    32221 tccaggcagg caggatcggc gttggcggca aaatggacgg cgatatgccg tctgaacggc
    32281 tcgagtgcct gcacgcacat tgccggcccg aggtcggatc cgcctatgcc gatgtggaca
    32341 aaatccgtaa tccgtttccc agttatcccc tgatacgaac cgtcgtccaa actgtgtgca
    32401 aactccaacg cacgatttaa ctcgcggcgg atttcgggca acacgtccct gccgtccgca
    32461 taaacggcat ccgcaccgtc gggtaggcgc aaagccgtat gcagcgcggc acgcccctcg
    32521 ctgccgttga ctttcgcacc cgtccgcaaa gcacgcattt tcccttccaa atccgccgtc
    32581 tccgcaagac ggcagagcag ttgcagcgta tcttcgccca aacggttttt gctgtaatcg
    32641 aacaacatcc cgtccaaacg ctcatgcata cgctcaaaac ggtcgggttc gcaggcaaag
    32701 cggtcgcgca aaaggacatg acgcgtatcc tgataatggc gttcgagcgc ataccatgca
    32761 cgggtaaaag cattcatctg ttttccttga tttttcagaa ccggattaaa atgtagcaga
    32821 atgtagttta acaaacggca gcggctttgg cgaatctccg gaacaccgcc cgccgcgtcc
    32881 aatagcacct tatttccata tggcgaaaat atagtgaatt aacaaaaatc aggacaaggc
    32941 ggcgagccgc aaacagtaca aatagtacgg aaccgattca cttggtgctt cagcacctta
    33001 gagaatcgtt ctctcttgag ctaaggcgag ccaacgctgt accggttttt gttaattcac
    33061 tataccctcc ggccggcatt tgacccgcaa caatatcctg caagatttat tgtgtacgca
    33121 taaatgccgg acagccgcct aaataaggta taatcgcatc cgattctgtc ccgtttgccg
    33181 cccgcaggcg ggatgtcggc ggttttcatc ttaccgggaa cacgccggta ctgtttttta
    33241 acgatgtttc ttacatttta ttccaattac tttacggggc tagaatatgg ctaaaaacgg
    33301 aggattttct ttgttcgcaa agaaagaaaa acgctttatc tttgaaggca ggcattccgc
    33361 ctccgacaaa ctggtcaacg gcgaagtatc cgcgtttacc gaagaagagg cgcgcaaaaa
    33421 actggcaaaa cgcggcatcc gcccgttgca gattacccgt gtgaaaacaa gctccaagcg
    33481 caaaatcaca caagaagaca tcaccgtttt cacccgccag ctgtccacga tgattaaagc
    33541 gggcctgccg ctgatgcagg catttgaaat cgtggcgcgc ggacacggca acccgtctat
    33601 gacggaaatg ctgatggaaa tccgaggcga agtggaacag ggcagctcgt tgagccgcgc
    33661 attctcaaac cacccaaaat atttcgaccg cttctactgc aatctggttg cggcgggcga
    33721 aacgggcggc gtattggaaa gcctgctgga caaattggca atttacaaag aaaaaaccca
    33781 ggccatccgc aaaaaggtaa aaaccgcact gacctatccg gtatccgtca tcgccgtcgc
    33841 catcggtttg gtattcgtga tgatgatttt cgtactgccc gcctttaaag aagtttacgc
    33901 caatatgggc gcggagcttc ccgcactgac ccaaacagtg atggatatgt ccgacttttt
    33961 cgtctcatac ggctggatgg tgctgatcgc actgggcttt gccatatacg gcttccttaa
    34021 attgaaggcg cgttcgatta aaatccaacg gcgtatggat gccatactgc tgcgtatgcc
    34081 gattttcgga gacattgtcc gcaaagggac gattgcccgc tggggcagga cgacggcgac
    34141 gctgattgcg gcaggcgtgc ctttggtcga tgtattggac tccactgccg gcgcggcggg
    34201 caatttaatc tatgaagaag ccacccggga aatccgtacg cgcgtcatcc aaggtttgtc
    34261 tatgacttcg gggatgcgtg cgacggaact gttccccaat atgatgttgc agatgtcttc
    34321 catcggcgag gaatcgggtt ctttggacga tatgctcaac aaagccgccg aattttacga
    34381 agacgaggtg gacaatgcgg tcggcaggct gtccgctatg atggagccga tcattatcgt
    34441 gattttaggc ttggtcatcg gcacgcttct ggtcgccatg tatctgccgc tgtttaattt
    34501 gggcaacgtg gtcgcctgat ttgccgcaca gatccggcgc ggattcgctc tgcgccggtt
    34561 tgtttttgtt ttgaatacat tgagaacaaa atatgtctga tttgtctgta ttgtcgccgt
    34621 ttgccgtgcc tttgacagcg gtgttcgggc tgctggtcgg aagtttctta aatgtcgtca
    34681 tttaccgcgt gccggtcatg atggaacgcg gctggacggt atttgccaaa gaatatttaa
    34741 acctgccgct gaccgaagag gaaagccgta ccttcaacct gatgaaaccg gattcctgct
    34801 gtcccaaatg ccgcgtgccg atacgcgcgt ggcagaacat cccgattgtc agctacctgc
    34861 tcctgcgcgg caaatgcgcc tcctgccaaa ccaaaatcag catacgctat cccttaatcg
    34921 aactgctgac cggcgtattg ttcgggctgg ttgcctggca atacggctgg tcttggatta
    34981 cattgggcgg tttgatactg accgcgtttc tgatttccct gacctttatc gatgcggaca
    35041 cccaatacct gcccgactcg atgacattac ccttgatttg gctggggctg atatttaatt
    35101 tggacggcgg cttcgtgcct ttgcagtctg ccgttttagg tgcggttgcc ggctatggtt
    35161 cgatatggct tttatgcgcg gtatataaac tgattaaagg cgaaatcggt atgggcaacg
    35221 gagatttcaa actgattgcc gcattgggcg cgtggctcgg catatccgca ttgcccgtgc
    35281 tgatttttgt ttcctcgctg atcggtttgg tcgcggcaat cgttatgcgc gtcgccaagg
    35341 ggcagcattt tgccttcggc cccgcactga cagtttcggg ctggataatt tttacggcaa
    35401 acgattccgt atggcgggcg gtcaactggt ggctgaccca tccggtgctg taagatgacg
    35461 gtatgggtcg gactgaccgg cggaatcggc agcggcaaat cggcagccgc gcaatgtttt
    35521 gccgatttgg gcgtaccgcg tatcgatgcg gatgcggcgg cgcactcgct gacggcttca
    35581 gacggcatcg ccctgccgga aatcaggcgg ctgttcggcg acaccgtttt cgacacacag
    35641 ggtttgttgc ggcgcgacat attgcgtaaa gaaatctttg cctccccatc gcgaaaagcc
    35701 ttgctcgaat ccgtgatgtt gccgctgatt ttctcagaaa tcaaaaaaca gcaagaaacc
    35761 tttaccgatg cggcttacgg cattgtcgaa attccgctgc tgacggaaaa gcgtcaattt
    35821 atcagcctga tacggcgtgt cctgaccata agcgcacctt tggaaaaacg tatcggcagg
    35881 gtgatggccc gcagcgggct gacgcgcggc gaggtggctg ccgtcatcag ccatcaggca
    35941 tccgaatccg aacgcctgct gcttgcagac gatgtactgc tcaatgacgg cagcctcaaa
    36001 agcctacgtg agaaaacaat gcgcctgcac gcgttttatt cagggatttt cgcctcaaaa
    36061 ccaacacaag gaaaacacaa tggctgaatc gcggcaaaca cgccttcaag tcaaatgtcc
    36121 gacctgtcaa acggcagtag tatggaaacc ggaaaacgca ttccgcccct tctgttcgca
    36181 acgctgcaaa ctgatcgact tgggcggatg ggcagatgag gaatatacgg ttacagccca
    36241 agaagaaggt ttattggaaa tatccgaacc cgacggcgca taccgctgac cgcaccccgt
    36301 cctccgacaa acccctgaaa atcaaatgcc gtctgaaaca aacacgcttc agacggcatt
    36361 ttcattctca aacctaatcg ttggtatttg ccgttacctc ttccaatgaa gtaatgccct
    36421 gcataacttt caaaataccg gcccggcgca aatccaccat accctcctta taggcaacgt
    36481 ccaaaatatc cacttccgta ccgttgttca taatcacacg ctgcatttct tcgctgatgg
    36541 gcataacctc atacacgccc gcacgcccct tataaccctg tccccggcaa cggtcgcaac
    36601 cgacggcgcg gtaaagtttc caatcttttg caaggtcctc atcggtgaag ccgacttcct
    36661 tcaaagcaga ggcagacggg cgttccactt cctgtttgca gctcgaacac agcctgcgta
    36721 aaagacgctg cgccataatc aggctgaccg aactggcaat attaaacggc gcgacaccca
    36781 tattcagcat acgcgacaac gtcgccggcg cattattcgt gtgcagtgtg gaaaacacca
    36841 tatgccctgt ttgtgccgcc ttaatcgcaa tatcggcagt ttccaaatca cgaatctcac
    36901 cgaccataat gatgtccggg tcctgacgca ggaaagactt caaagcagcg gcaaaagtca
    36961 ggccctgctt atcattgacg ttaacctgat tgatgcccgg caggttaatc tcggcagggt
    37021 cttccgccgt tgcaatattt accgactccg tattcaaaat attcaaacag gtatagagcg
    37081 acaccgtctt acccgaaccc gtcggaccgg ttaccagcac catcccgtag ggacggtgaa
    37141 tcgcttccaa caacaatttt ttctgaaacg gctcaaaacc gagctggtcg atgttcaaag
    37201 acgcggcatc ggaattcaaa atccgcatca cgaccttttc gccaaacagc gtcggcaatg
    37261 tgctgacacg gaaatcgaca ggcttgccgc ccttttgaaa ggtcagctgc atcctgccgt
    37321 cctgcggtat ccgtttttcg gaaatgtcca aacgcgacat taccttaatc cgggaagcaa
    37381 gctgccccct taccgcaatg ggcggctgaa ccacctcgcg gagctgcccg tccacacgga
    37441 aacggatacg ggcattgtgt tcgtaaaact cgaaatggat gtccgatgcc ccgctgcgca
    37501 aggcatccga caaagtctta tggataaacc tcggaacagg gccgtcttct gcctcctcgt
    37561 tgtcgatata cagggtgtgg ctttcctctt cctcctgccc ctccccaagc tcctgaagca
    37621 gcgatgtcga acgcgaaccc acccaatcga gcaaacccgc caactggtca tcctcgacaa
    37681 tgaccaactc aacctcaatc cctgcggcag aaacggtttt ctgaatttgc ggcatctgtg
    37741 tcggatcgga aaccgcaaaa aatactttgt cgccccgacg gaaaaccggc acacagtgga
    37801 actccaccat ctgctcctcc gtcaacaccc ccatcagcac cctgtggcgc ggataatgac
    37861 gcaaatcaag aatcgaataa ctgaacaccc tcgcaatcaa tgccgcaagc gacttgggcg
    37921 aaatgacacc gtctgaaaac agcatcggca acacttcctt acccgcctgc gactcattgt
    37981 agtaatgctc ggcctgctca acagtaacca cctggttttg aaccagaatc ctcagcaaac
    38041 ctacgctcat acgaccttat ccccaaattt attcattgtt atacctgtac agcttttata
    38101 gtggattaaa tt
//
LOCUS       DAWFIE010000010        37019 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWFIE010000010 DAWFIE010000000
VERSION     DAWFIE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.010"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            63..611
                     /locus_tag="WGQ71_000463"
     CDS             63..611
                     /locus_tag="WGQ71_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0706529.1"
                     /translation="MPIEDVFSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTC
                     TGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSK
                     EEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEG
                     LRFAIREGGRTVGAGVVSSVIA"
     gene            629..940
                     /gene="rpsJ"
                     /locus_tag="WGQ71_000464"
     CDS             629..940
                     /gene="rpsJ"
                     /locus_tag="WGQ71_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ0706530.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            1061..1366
                     /locus_tag="WGQ71_000465"
                     /pseudo
     CDS             1061..1366
                     /locus_tag="WGQ71_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1630..2274
                     /gene="rplC"
                     /locus_tag="WGQ71_000466"
     CDS             1630..2274
                     /gene="rplC"
                     /locus_tag="WGQ71_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ0706531.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGISKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            2274..2894
                     /gene="rplD"
                     /locus_tag="WGQ71_000467"
     CDS             2274..2894
                     /gene="rplD"
                     /locus_tag="WGQ71_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ0706532.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            2891..3205
                     /gene="rplW"
                     /locus_tag="WGQ71_000468"
     CDS             2891..3205
                     /gene="rplW"
                     /locus_tag="WGQ71_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ0706533.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLASATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRRDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            3211..4044
                     /gene="rplB"
                     /locus_tag="WGQ71_000469"
     CDS             3211..4044
                     /gene="rplB"
                     /locus_tag="WGQ71_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ0706534.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     SNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            4051..4329
                     /gene="rpsS"
                     /locus_tag="WGQ71_000470"
     CDS             4051..4329
                     /gene="rpsS"
                     /locus_tag="WGQ71_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ0706535.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            4338..4667
                     /gene="rplV"
                     /locus_tag="WGQ71_000471"
     CDS             4338..4667
                     /gene="rplV"
                     /locus_tag="WGQ71_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ0706536.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            4677..5369
                     /gene="rpsC"
                     /locus_tag="WGQ71_000472"
     CDS             4677..5369
                     /gene="rpsC"
                     /locus_tag="WGQ71_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ0706537.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            5353..5769
                     /gene="rplP"
                     /locus_tag="WGQ71_000473"
     CDS             5353..5769
                     /gene="rplP"
                     /locus_tag="WGQ71_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ0706538.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            5769..5960
                     /gene="rpmC"
                     /locus_tag="WGQ71_000474"
     CDS             5769..5960
                     /gene="rpmC"
                     /locus_tag="WGQ71_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ0706539.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            5960..6223
                     /gene="rpsQ"
                     /locus_tag="WGQ71_000475"
     CDS             5960..6223
                     /gene="rpsQ"
                     /locus_tag="WGQ71_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ0706540.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            6445..6813
                     /gene="rplN"
                     /locus_tag="WGQ71_000476"
     CDS             6445..6813
                     /gene="rplN"
                     /locus_tag="WGQ71_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ0706541.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6825..7148
                     /gene="rplX"
                     /locus_tag="WGQ71_000477"
     CDS             6825..7148
                     /gene="rplX"
                     /locus_tag="WGQ71_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ0706542.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            7158..7697
                     /gene="rplE"
                     /locus_tag="WGQ71_000478"
     CDS             7158..7697
                     /gene="rplE"
                     /locus_tag="WGQ71_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ0706543.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            7700..8005
                     /gene="rpsN"
                     /locus_tag="WGQ71_000479"
     CDS             7700..8005
                     /gene="rpsN"
                     /locus_tag="WGQ71_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ0706544.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            8021..8413
                     /gene="rpsH"
                     /locus_tag="WGQ71_000480"
     CDS             8021..8413
                     /gene="rpsH"
                     /locus_tag="WGQ71_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ0706545.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            8427..8960
                     /gene="rplF"
                     /locus_tag="WGQ71_000481"
     CDS             8427..8960
                     /gene="rplF"
                     /locus_tag="WGQ71_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ0706546.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            8974..9327
                     /gene="rplR"
                     /locus_tag="WGQ71_000482"
     CDS             8974..9327
                     /gene="rplR"
                     /locus_tag="WGQ71_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ0706547.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            9346..9864
                     /gene="rpsE"
                     /locus_tag="WGQ71_000483"
     CDS             9346..9864
                     /gene="rpsE"
                     /locus_tag="WGQ71_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ0706548.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            9857..10042
                     /gene="rpmD"
                     /locus_tag="WGQ71_000484"
     CDS             9857..10042
                     /gene="rpmD"
                     /locus_tag="WGQ71_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ0706549.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            10044..10478
                     /gene="rplO"
                     /locus_tag="WGQ71_000485"
     CDS             10044..10478
                     /gene="rplO"
                     /locus_tag="WGQ71_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ0706550.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            10490..11800
                     /gene="secY"
                     /locus_tag="WGQ71_000486"
     CDS             10490..11800
                     /gene="secY"
                     /locus_tag="WGQ71_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ0706551.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            11805..12023
                     /gene="infA"
                     /locus_tag="WGQ71_000487"
     CDS             11805..12023
                     /gene="infA"
                     /locus_tag="WGQ71_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ0706552.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            12044..12157
                     /gene="rpmJ"
                     /locus_tag="WGQ71_000488"
     CDS             12044..12157
                     /gene="rpmJ"
                     /locus_tag="WGQ71_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ0706553.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            12223..12585
                     /gene="rpsM"
                     /locus_tag="WGQ71_000489"
     CDS             12223..12585
                     /gene="rpsM"
                     /locus_tag="WGQ71_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ0706554.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            12605..13000
                     /gene="rpsK"
                     /locus_tag="WGQ71_000490"
     CDS             12605..13000
                     /gene="rpsK"
                     /locus_tag="WGQ71_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ0706555.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            13020..13640
                     /gene="rpsD"
                     /locus_tag="WGQ71_000491"
     CDS             13020..13640
                     /gene="rpsD"
                     /locus_tag="WGQ71_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ0706556.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            13666..14652
                     /gene="rpoA"
                     /locus_tag="WGQ71_000492"
     CDS             13666..14652
                     /gene="rpoA"
                     /locus_tag="WGQ71_000492"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ0706557.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            14676..15044
                     /gene="rplQ"
                     /locus_tag="WGQ71_000493"
     CDS             14676..15044
                     /gene="rplQ"
                     /locus_tag="WGQ71_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ0706558.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHEAIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            15191..15904
                     /gene="minC"
                     /locus_tag="WGQ71_000494"
     CDS             15191..15904
                     /gene="minC"
                     /locus_tag="WGQ71_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ0706559.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            15933..16748
                     /gene="minD"
                     /locus_tag="WGQ71_000495"
     CDS             15933..16748
                     /gene="minD"
                     /locus_tag="WGQ71_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ0706560.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            16752..17015
                     /gene="minE"
                     /locus_tag="WGQ71_000496"
     CDS             16752..17015
                     /gene="minE"
                     /locus_tag="WGQ71_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ0706561.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            17019..17939
                     /gene="oxyR"
                     /locus_tag="WGQ71_000497"
     CDS             17019..17939
                     /gene="oxyR"
                     /locus_tag="WGQ71_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ0706562.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(17936..18127)
                     /locus_tag="WGQ71_000498"
     CDS             complement(17936..18127)
                     /locus_tag="WGQ71_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706563.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(18269..21106)
                     /locus_tag="WGQ71_000499"
     CDS             complement(18269..21106)
                     /locus_tag="WGQ71_000499"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ0706564.1"
                     /translation="MLDKYNPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQQGFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQVEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVDRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESTDDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(21203..22012)
                     /gene="zupT"
                     /locus_tag="WGQ71_000500"
     CDS             complement(21203..22012)
                     /gene="zupT"
                     /locus_tag="WGQ71_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ0706565.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(22193..23449)
                     /locus_tag="WGQ71_000501"
     CDS             complement(22193..23449)
                     /locus_tag="WGQ71_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ0706566.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQTKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIASGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(23528..24919)
                     /locus_tag="WGQ71_000502"
     CDS             complement(23528..24919)
                     /locus_tag="WGQ71_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0706567.1"
                     /translation="MQLFLDNLKAFFETISGWVWGPVMLVLLVGTGILLTVLLKGLQF
                     TMLGYALKQAFVPSKKHEDGEDHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVTNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(25276..26052)
                     /gene="lpxA"
                     /locus_tag="WGQ71_000503"
     CDS             complement(25276..26052)
                     /gene="lpxA"
                     /locus_tag="WGQ71_000503"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ0706568.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTRLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(26139..26588)
                     /gene="fabZ"
                     /locus_tag="WGQ71_000504"
     CDS             complement(26139..26588)
                     /gene="fabZ"
                     /locus_tag="WGQ71_000504"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ0706569.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(26622..27668)
                     /gene="lpxD"
                     /locus_tag="WGQ71_000505"
     CDS             complement(26622..27668)
                     /gene="lpxD"
                     /locus_tag="WGQ71_000505"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ0706570.1"
                     /translation="MIPATYTLSQITAQLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPSATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(27701..28201)
                     /locus_tag="WGQ71_000506"
     CDS             complement(27701..28201)
                     /locus_tag="WGQ71_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ0706571.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(28267..30660)
                     /gene="bamA"
                     /locus_tag="WGQ71_000507"
     CDS             complement(28267..30660)
                     /gene="bamA"
                     /locus_tag="WGQ71_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ0706572.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKIGDAYNDGRGGEIIKSLYATGFFDDVRVESADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGERFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEIGYANGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(30717..32057)
                     /gene="rseP"
                     /locus_tag="WGQ71_000508"
     CDS             complement(30717..32057)
                     /gene="rseP"
                     /locus_tag="WGQ71_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ0706573.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAASTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPITSWQEWANLTRQSPGRKIALTYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(32092..33327)
                     /locus_tag="WGQ71_000509"
     CDS             complement(32092..33327)
                     /locus_tag="WGQ71_000509"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ0706574.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSLHP
                     EKFRVFALAGHKQVEKLAVQCRTFRPEYAVVADAEHAARLEALLKGDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPD
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLAQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(33332..34129)
                     /locus_tag="WGQ71_000510"
     CDS             complement(33332..34129)
                     /locus_tag="WGQ71_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ0706575.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLSGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(34132..34878)
                     /locus_tag="WGQ71_000511"
     CDS             complement(34132..34878)
                     /locus_tag="WGQ71_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ0706576.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFSRQILKGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAKGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(34934..35491)
                     /gene="frr"
                     /locus_tag="WGQ71_000512"
     CDS             complement(34934..35491)
                     /gene="frr"
                     /locus_tag="WGQ71_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ0706577.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAVKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(35671..36798)
                     /locus_tag="WGQ71_000513"
     CDS             complement(35671..36798)
                     /locus_tag="WGQ71_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ0706578.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(36874..36949)
                     /locus_tag="WGQ71_000514"
     tRNA            complement(36874..36949)
                     /locus_tag="WGQ71_000514"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36914..36916),aa:Thr,seq:cgt)
BASE COUNT         8757 a         8444 c        10073 g         9745 t
ORIGIN      
        1 tggctgccgc attggacagc tacatcccga ctcccgagcg tgccgtggac aaaccgttcc
       61 tgctgcctat cgaagacgtg ttctccattt ccggccgcgg tacagtagta accggccgtg
      121 tagagcgcgg tatcatccac gttggtgacg agatcgaaat cgtcggtctg aaagaaaccc
      181 aaaaaaccac ctgtaccggc gttgaaatgt tccgcaaact gctggacgaa ggtcaggcgg
      241 gcgacaacgt aggcgtattg ctgcgcggta ccaaacgtga agacgtagag cgtggtcagg
      301 tattggccaa accgggtact atcactcctc acaccaagtt caaagcagaa gtgtacgtac
      361 tgagcaaaga agagggcggc cgccataccc cgttcttcgc caactaccgt ccccaattct
      421 acttccgtac caccgacgta accggcgcgg ttactttgga agaaggtgtg gaaatggtaa
      481 tgccgggtga gaacgtaacc attaccgtag aactgattgc gcctatcgct atggaagaag
      541 gcctgcgctt tgcgattcgc gaaggcggcc gtaccgtggg tgccggcgtg gtttcttctg
      601 ttatcgctta attgaaggat attgataaat ggcaaaccaa aaaatccgta tccgcctgaa
      661 agcttatgat tacgccctga ttgaccgttc tgcacaagaa atcgttgaaa ctgcaaaacg
      721 taccggtgcc gttgtaaaag gcccgattcc tttgccgacc aaaatcgaac gtttcaacat
      781 tctgcgttct ccgcacgtga acaaaacttc ccgtgaacaa ttggaaatcc gcacccactt
      841 gcgcctgatg gacatcgtgg attggaccga taaaactacc gatgcgctga tgaagctgga
      901 tttgccggcc ggtgttgatg tagaaatcaa agtccaataa ttcggactat tgaaaatccc
      961 caagcaatca atgcttgggg attttttatg ttatgccgag acctttgcaa aattccccaa
     1021 aatcccctaa attcccacca agacatttag gagattttcc atgagcacct tctttcaaca
     1081 aaccgcacaa gccatgattg ctaaacacat cgaccgcttc ccgctattga agttggaccg
     1141 ggtgattgat tggcagctga tcgaacaata cctgaaccgt caaaaaaccc gttaccttag
     1201 agaccaccgc ggccgtcccg cctatcccct gctatccatg ttcaaagccg tcctgctcgg
     1261 acaatggcac agcctctccg atcccgaact cgagcacagc ctcatcaccc gcatcgattt
     1321 caacctgttt tgccgctttg acgaactgag catccccgat tacagtcatc aaccatattc
     1381 cggtttgtcg gagaaagatg catacgctgt gatgaccgga taccgaccct ttaaaagagt
     1441 ccgaccctat gccgtctgaa aattcaaaac gcttcagacg gcatattgaa gatatttctg
     1501 atatttctgt tgatatttct ttaacttgtc agatataatg ccgagcttgg tacatttgtg
     1561 ccaagtttaa ctttgtctga aagacaggcc aatcgtagcc tgtcccttta ctttaaaagg
     1621 aaaataatca tgactttagg tctggttgga cgcaaagttg gtatgacccg cgtgttcgac
     1681 gaacagggtg tttctgttcc ggtaaccgtt ttggatatgt ctgccaaccg cgttacacaa
     1741 gtaaaatcca aagatactga cggctatact gccgttcaag ttacctttgg tcagaaaaaa
     1801 gccaatcgtg tcaacaaagc cgaagccggg cactttgcaa aagcaggtgt tgaagccggt
     1861 cgcggtttga ttgagtttgc tttgactgaa gaaaaactgg ctgaattgaa agctggtgac
     1921 gaaatcaccg tttctatgtt tgaagtcggt caactggtcg atgtaaccgg tatctctaaa
     1981 ggtaaaggtt tctccggcac gattaaacgt cataacttcg gtgcccaacg tacttcccac
     2041 ggtaactccc gttctcaccg cgttccaggt tctatcggta tggcgcaaga cccgggtcgc
     2101 gtgttccccg gtaaacgcat ggccggccaa tacggcaaca ccaaagcaac tgttcaaaaa
     2161 ttggaagttg tccgtgttga tgcagaacgc caactgctgt tggttaaggg tgctgttccg
     2221 ggtgcggtca acagcgatgt tgtagttcgt cccagcgtga aagtaggtgc gtaatggaat
     2281 tgaaagtaat tgacgctaaa ggacaagttt caggcagcct gtctgtttct gatgctttgt
     2341 tcgcccgcga atacaatgaa gcgttggttc atcagctggt aaatgcctac ttggcaaacg
     2401 cccgctctgg taaccgtgct caaaaaaccc gtgccgaagt aaaacactca accaaaaaac
     2461 catggcgtca aaaaggtacc ggccgcgccc gttccggtat gacttcatct ccgctgtggc
     2521 gtaaaggtgg tcgcgcgttc ccgaacaaac ccgacgaaaa cttcactcaa aaagtaaacc
     2581 gcaaaatgta ccgtgccggt atggcgacta ttctgtccca attgactcgt gacgagcgtt
     2641 tgtttgcgat tgaggcgttg actgccgaaa ctcctaaaac caaagttttt gctgaacaag
     2701 tgaaaaatct gggtctggag caagtgttgt ttgtaaccaa acagctcgac gagaatgttt
     2761 acttggcttc acgcaacttg ccaaacgtgt tggttttgga agctcaacaa gttgatcctt
     2821 acagcttgct gcgttacaaa aaagtaatca tcactaaaga tgcagttgca caattagagg
     2881 agcaatgggt atgaatcaac aacgtttgac tcaagtgatt ttggcaccta tcgtttctga
     2941 aaaaagcaac gtattggctg aaaaacgcaa ccaaatgacg tttaaagtct tggcaagtgc
     3001 aaccaaacct gaaatcaaag cggctgtcga gctgctgttt ggtgttcaag ttgcttctgt
     3061 aactaccgtt acaactaaag gcaaaactaa gcgttttggt cgtactttgg gccgccgccg
     3121 cgatgttaaa aaagcttatg taagcttggc tgccggtcaa gagttggatt tggaagccgc
     3181 tgctgcagct gcagataagg aataaacaaa atggcaatcg ttaaaatgaa gccgacctct
     3241 gcaggccgtc gcggcatggt tcgcgtggta acagaaggtt tgcacaaagg tgcgccttat
     3301 gcacctttgc tcgaaaagaa aaattctact gctggtcgta acagtaatgg tcatatcacc
     3361 acccgtcaca aaggcggcgg tcataaacac cattaccgtg ttgtagactt taaacgtaac
     3421 aaagacggca ttcctgccaa agtagagcgt atcgaatacg atcctaaccg tactgccttc
     3481 atcgcactgt tgtgctatgc agacggcgag cgtcgttaca tcattgctcc tcgcggtatt
     3541 caagccggtg ccgtattggt ttccggtgct gaagctgcca tcaaagtagg caacaccctg
     3601 ccgattcgca acattcccgt tggtacgact atccactgta tcgaaatgaa acccggtaaa
     3661 ggtgctcaaa tcgcacgttc tgccggtgct tctgcggtat tgctggctaa agaaggcgcg
     3721 tacgctcaag tccgcctgcg ctctggcgaa gtccgtaaaa tcaacgtaaa ttgccgtgcg
     3781 accatcggtg aagttggtaa cgaagagcaa agcctgaaaa aaatcggtaa agccggtgcc
     3841 aaccgttggc gcggtattcg tccgaccgta cgcggtgttg tcatgaatcc tgtcgatcac
     3901 ccgcatggtg gtggtgaagg ccgtacgggc gaggcccgcg aaccggtcag cccatggggt
     3961 actcctgcta aaggctaccg cactcgtaat aacaaacgca cggataacat gattgttcgt
     4021 cgccgttact caaataaagg ttaatttagt atggctcgtt cattgaaaaa aggcccatat
     4081 gtagacctgc atttgctgaa aaaagtagat gctgctcgcg caagcaacga caaacgcccg
     4141 attaaaacct ggtctcgtcg ttctaccatt ctgcctgatt ttatcggtct gaccattgct
     4201 gtgcacaacg gccgcaccca tgtgcctgtg tttatcagcg acaatatggt tggtcataaa
     4261 ttaggcgaat tctcattgac ccgtaccttt aaaggccact tggccgataa aaaggctaaa
     4321 aagaaataag gtgaatcatg agagtaaatg cacaacataa aaatgcccgt atctctgctc
     4381 aaaaggctcg tttggtagct gatttgattc gtggtaaaga cgttgcccaa gctttgaata
     4441 ttttggcttt cagtcctaaa aaaggtgccg agctgattaa aaaagtattg gagtcagcta
     4501 ttgctaatgc cgagcacaat aacggtgcgg acattgatga actgaaagtg gtaactatct
     4561 ttgttgacaa aggcccaagc ttgaaacgtt ttcaagctcg cgccaaaggt cgcggtaacc
     4621 gcatcgaaaa acaaacttgt catatcaatg tgacagtggg taactaagga aaagctatgg
     4681 gacaaaagat taaccctaca ggctttcgcc tggcggtaac taaagactgg gcttcaaaat
     4741 ggtttgctaa aagcaccgac ttttctactg ttttgaagca ggatatcgat gttcgcaatt
     4801 atttgcgtca aaaattggcc aatgcttcgg ttggtcgagt ggttattgaa cgccctgcaa
     4861 aatctgcacg cattaccatt cactccgctc gtccgggtgt ggttatcggt aaaaaaggtg
     4921 aggatatcga ggttttgaaa cgtgacttgc aagtcttgat gggtgtacct gttcatgtaa
     4981 atattgaaga gatccgccgt cctgagttgg atgctcaaat tattgctgac ggtattgccc
     5041 agcagttgga aaagcgcgtt caattccgtc gtgctatgaa acgagcaatg caaaatgcaa
     5101 tgcgttctgg tgctaaaggc attaagatta tgacttcagg ccgtctgaat ggtgcggata
     5161 ttgcccgtag cgaatggtat cgtgaaggtc gcgtgccact gcatacttta cgtgcaaatg
     5221 tagattatgc aaccagcgaa gcgcacacca catatggtgt attgggtctg aaagtttggg
     5281 tttatacgga aggcaatatt aaatcttcca aacctgaaca tgagagtaaa caaagaaagg
     5341 caggtagacg taatgctgca gccaactaga ctgaaatacc gtaagcaaca aaagggtcgc
     5401 aataccggca tcgctactcg cggtaataag gtaagtttcg gtgagttcgg cttgaaagcc
     5461 gtaggtcgtg gtcgtttgac tgcccgtcaa atcgaagctg ctcgtcgtgc aatgacccgt
     5521 catatcaaac gtggtggtcg tatttggatt cgtgtattcc ctgataaacc gattactgaa
     5581 aagcctattc aagttcgtat gggtggcggt aaaggtaacg tggaatatta cattgccgaa
     5641 attaaaccag gtaaagtgtt gtatgaaatg gatggcgttc cagaggaact ggctcgtgaa
     5701 gcattcgagt tggctgctgc caaattgcct attcctacaa cctttgtagt aagacaggtg
     5761 ggtcaataat gaaagcaaat gaattgaaag acaaatccgt tgagcagttg aatgcagatt
     5821 tgttggactt gttgaaagct cagtttggct tacgtatgca aaacgctacc ggtcaattag
     5881 gcaaaccaag tgaattgaaa cgtgtacgtc gcgatattgc tcgtattaaa accgttttaa
     5941 ctgaaaaagg tgctaagtaa tgagcgaaac taaaaatgtt cgtactttgc aaggcaaagt
     6001 agtaagcgac aaaatggata aaaccgtaac agtattggtt gagcgtaaag taaaacatcc
     6061 gctttatggt aagattattc ggttatctac taaaatccat gcccatgatg aaaataatca
     6121 atatggaatt ggtgatgtgg ttgttatatc ggaatcccgt ccattgtcaa aaactaaatc
     6181 ttgggttgtc agtgagctgg ttgagaaagc acgttctatt taagaattaa agcaacgtgc
     6241 ttggaatggg aaacgaagta ttgcagcaaa tttaatttgc gtgtaaactt cgtttcctgt
     6301 ctttcagttt cttctggaag tttcttccct ttcggggtcc aagactggtt tacttgaacc
     6361 gcaaggtttc atttaataag cagcggcttt gctgtaagtt atctgaaagt ggtaaattaa
     6421 gttggttaat ttaaaggtaa taacatgatt caaatgcaga ccatcttaga tgtggctgat
     6481 aactctggtg cgcgtcgcgt aatgtgtatc aaggtattgg gcggatctaa gcgtcgctac
     6541 gcttctgttg gcgatattat taaagtggca gttaaagatg cggctccgcg tggccgtgtc
     6601 aaaaaaggcg atgtatataa tgcggtagtt gttcgtactg ctaagggtgt acgtcgtcct
     6661 gatggtgcgt taattaaatt cgataacaat gccgccgtgt tactgaataa taaacttgaa
     6721 cctttgggta ctcgtatctt tggtccggta acccgtgaat tgcgtactga gcgatttatg
     6781 aaaatcgttt cattggcacc tgaagtatta taaggaatgg cacgatgaat aaaatcatta
     6841 aaggcgatag ggttgtagta attgctggta aggataaagg taagcagggt caagtagttc
     6901 gagtgttggg tgataaagtt gttgttgagg gcgttaatgt tgtaaaacgc catcaaaaac
     6961 ctaatccaat gcgtggcatt gagggcggta ttattactaa agaaatgcct ttggatattt
     7021 ctaatatcgc aatcctgaat ccggaaacta ataaagcgga ccgtgttggt attaagctga
     7081 ttgaaaatga aggcaaagtt aaacgcgttc gtttcttcaa atcaaatggc tctatcattg
     7141 gggcataagg agataacatg gctcggttga gagagtttta taaagagaca gttgttcctg
     7201 aattggttaa acaatttggt tacaaatcag taatggaagt cccgcgtatt gaaaaaatta
     7261 ccttgaatat gggtgtgggt gaggctgttg ctgataaaaa agttatggaa catgctgttt
     7321 ccgatttaga gaaaattgcc ggtcaaaaac cggttgttac tgttgcccgt aaatctatcg
     7381 caggttttaa aatccgtgat aactatccgg ttggttgcaa agtaacattg cgtcgtgatc
     7441 aaatgtttga attcttggat cgtttgatta ctattgcatt acctcgcgta cgtgacttcc
     7501 gtggtgtgag cggtaaatca tttgatggcc gtggcaatta caatatgggt gttcgtgagc
     7561 aaattatttt tccggaaatt gaatacgata aaattgatgc tttgcgtggt ttgaatatta
     7621 ctattactac tacagcaaaa accgatgagg aagcgaaagc tttattgtca ttgtttaaat
     7681 ttccgttcaa aggataatca tggctaagaa agcacttatt aatcgtgatc tgaaacgtca
     7741 agctttggct aaaaaatatg cggctaaacg cgcggcaatt aaagcggtaa tcaatgattc
     7801 gaatgcaact gaggaagagc gttttgaggc tcgtttgagg tttcaatcca ttcctcgtaa
     7861 tgcggcacct gtgcgtcaac gtcgtcgttg tgctttgaca ggtcgccctc gtggtacttt
     7921 ccgtaaattt ggtttgggtc gtattaaaat ccgtgaaatc gccatgcgtg gcgaaattcc
     7981 gggtgttgtt aaagccagct ggtaatagga gtaattaaga atgagtatgc atgatcctat
     8041 ttccgatatg ttgactcgta tccgcaatgc gcaacgtgct aataaagcag cggttgcaat
     8101 gccttcttca aaattaaagt gtgctattgc aaaggtattg aaagaagaag gatatattga
     8161 ggacttcgca gtttcatctg acgtaaagtc tatattggaa attcaattaa aatactatgc
     8221 aggtcgtcct gtaattgaac aaatcaagcg tgtatctcgc cctggtttgc gtatttataa
     8281 agcgtctagt gagattccaa gtgttatgaa tggcttgggt attgctattg ttagtacttc
     8341 taaaggtgta atgactgatc gtaaagcacg ttctcaaggt gttggtggtg agttgttatg
     8401 cattgtagcc tagtggagga aaagaaatgt cacgtgtcgc aaaaaaccca gtgactgttc
     8461 ccgctggtgt agaagtaaaa tttggaacag aggcattagt tattaagggt aagaacggtg
     8521 aattgtcttt tcctttgcat tctgatgtag ccattgaatt taatgatggc aaattggctt
     8581 ttgttgcgaa taacagcagt aaacaagcaa atgcaatgtc tggtactgct cgcgcattag
     8641 tcagcaatat ggttaaaggt gtttcagaag gttttgagaa aagattgcaa ttgataggtg
     8701 tgggttatcg tgctcaagca caaggtaaaa tcttgaatct gtctttgggt ttttctcatc
     8761 cgatcgtata tgaaatgcct gaaggtgtct ccgttcaaac tcctagccaa acagagattg
     8821 ttttaaccgg ctcggataaa caagttgttg gtcaagttgc tgctgagatt cgtgcgttcc
     8881 gtgctcctga gccttataaa ggtaaaggtg ttcgctatgt aggagaagta gtggtaatga
     8941 aagaagccaa gaaaaaataa ttgaggttca ctaatggata aacatacaac ccgactccgt
     9001 cgtgcacgca aaacccgtgc tcgtattgcg gacttgaaaa tggtaagatt atgtgtgttc
     9061 cgaagcaata atcatattta tgctcaagta attagtgctg aaggtgataa agtattggct
     9121 caagcctcta cattggaagc tgaggtgcgc ggtagtctga aatctggaag caatgttgaa
     9181 gcagctgcaa tagttggtaa acgtattgct gagaaggcta aagcagcagg tgtagaaaag
     9241 gttgcttttg atcgttcagg tttccaatat cacggtcgtg tgaaggcttt ggctgaagct
     9301 gctcgtgaaa atggtttaag cttctaaata tttggagact ttcagatggc aaaacatgaa
     9361 attgaagaac gcggtgacgg tctgattgaa aagatggtcg cagttaaccg tgtaaccaaa
     9421 gtagttaaag gtggtcgcat tatggctttc tctgcgctaa ctgttgttgg tgatggagat
     9481 ggtcgtattg gtatgggtaa aggtaagtca aaagaagtgc ctgttgcagt ccaaaaggcg
     9541 atggatcaag cacgacgctc tatgattaag gtaccattaa aaaatggtac gattcatcat
     9601 gaggttattg gtcgacatgg tgctactaaa gtatttatgc agcctgctaa agagggtagt
     9661 ggcgtaaaag ccggtggacc tatgcgtttg gtttttgatg ctatgggcat tcataatatc
     9721 tccgccaaag tgcacggatc tactaaccca tataatatcg tacgtgcaac attagatggt
     9781 ttgtctaagt tgcatactcc tgctgatatc gcagccaaac gtggcttgac agtggaagac
     9841 attttgggag ttaaccatgg ctgaacaaaa aaagattagg gttacattgg ttaaaagcct
     9901 gattggtaca attgaatctc atcgtgcatg tgcacgcggt ttaggtttgc gtcgtcgcga
     9961 gcatacggta gaggttttag atacccctga aaaccgtggt atgattaata aaatcagcta
    10021 cttgttgaaa gtggagtctt gatatgtttt tgaatacaat tcaacctgct gttggtgcta
    10081 cgcatgctgg tcgtcgtgtt ggacgcggta ttggtagtgg tcttggcaaa acgggtggtc
    10141 gtggtcataa aggtcaaaag agccggtctg gtgggtttca taaggtgggt ttcgagggtg
    10201 gtcaaatgcc cttgcaacga cgcctcccta aaagaggttt taaatcttta acagcatcag
    10261 ctaatgcaca gcttcgttta agtgaactgg aatcaattgc tgttaatgag attgatattt
    10321 tggtcttaaa gcaagcgggt ctgattgcat ctacagtctc taatgttaaa gttattgctt
    10381 ctggtgaaat ttctaaggca gttgctttga agggtattaa agttaccaaa ggtgcgagag
    10441 ctgctatcga ggctgttggt ggtaagattg aaatgtaagg tttaatattg tggctaatca
    10501 acaaacgtca tcaggttcat ccaaatttgg agatcttaag aaacgtcttt tgtttctatt
    10561 tggagcattg attgtttttc gaattggtgc ccatataccc gtacctggag ttgatgctgt
    10621 tgctttagct aaattatacg aaagcgctgg aaacggcatc ctgggaatat tgaatatgtt
    10681 ttccggtggg tcgttagagc gctttagtat atttgcaata ggaattatgc catatatttc
    10741 agcttctatt attgtacagc ttgcttctga aattttgcca tcattgaagg ctttaaaaaa
    10801 agaaggggag gctggtagaa aggtaattac gaaatatact aggtatggta ctgttttgtt
    10861 agcaattctt caaagtctag gtgttgcatc tttcgtattt cagcaaggaa ttgttgtaac
    10921 aagttcattt gagtttcatg tttccacggt agtttctttg gtaacgggaa ccatgtttct
    10981 tatgtggctt ggggagcaaa ttactgaaag gggtatcggg aacggtattt ctttaatcat
    11041 tacggcaggt attgcttcag gtattccttc gggtattgca aagctggtta cactgacgaa
    11101 ccaaggttct atgagcatgc ttacggcgtt gtttattgta tttggtgcct tattattaat
    11161 ttatttggtt gtatactttg aaagtgcaca gcggaagatt cctattcatt atgcaaaacg
    11221 ccagtttaat ggtagggcgg gtagtcaaaa tacgcatatg cctttcaagt tgaatatggc
    11281 tggtgttatt cccccaattt ttgcttccag tattattcta tttccatcta ctcttttagg
    11341 ttggtttggt tcggctgata caaatagtgt tttgcacaaa atagctggat tgttacaaca
    11401 cggtcaattg ctgtatatgg ctttatttgc agcgacagtt attttctttt gttattttta
    11461 tacggctttg gtttttagcc ctaaagaaat ggcagagaat ttaaaaaaga gtggtgcttt
    11521 tgttcctggg attagacctg gtgagcagac ctctaggtat ttagaaaaag ttgtattacg
    11581 tttgacattg tttggagctc tttatattac aactatttgt ttaattccag agttcttaac
    11641 tacggtttta aatgtacctt tttatttggg tggcacgtct ttgttgattc tagttgttgt
    11701 aacgatggat tttagtacac aaataaattc gtataggctt actcaacagt atgataagtt
    11761 aatgactcgt tcagaaatga aatcattttc tcggaaatag aattatggcg aaagaagata
    11821 ctatccaaat gcaaggtgaa attcttgaaa ctttacctaa tgcaacattt aaagtaaaac
    11881 ttgagaatga ccatattgta ttgggtcata tttctgggaa gatgcggatg cattacattc
    11941 gtatttctcc gggagataag gtcacagtag agctgacacc ttatgatcta actagggctc
    12001 gaatcgtttt cagagcaaga taaaccaata aaaggaaaat aaaatgcgtg tacaaccatc
    12061 tgttaagaaa atttgccgaa attgcaagat tattcgtcga aatcgtgtag ttcgtgtaat
    12121 ttgtactgat ctccgtcaca aacagcgtca aggttaatgg aatatttctt ttaatgtgat
    12181 tctgtgatat agtgacacac tttgccctaa aaaggaaaaa atatggctcg tattgcaggg
    12241 gtaaatatcc ctaataacgc acacatcgta attggtcttc aggctattta cggtattggt
    12301 gctactcgtg ctaaattgat ttgtgaggct gcaaatattg cgcctgatac taaagcaaaa
    12361 gatttggacg agactcaatt agatgctttg cgtgaccaag ttgccaagta tgaagtagaa
    12421 ggtgatttgc gtcgtgaggt aactatgagt atcaagcgat tgatggacat gggctgctat
    12481 cgtggcttcc gtcatcgtcg cggcttacca tgccgcggtc aacgcactcg tacaaatgcg
    12541 cgtacccgca aaggtccgcg taaagcgatt gctggtaaga aataaatttt aaggaatttt
    12601 attaatggct aaagcaaaca cagcttcacg tgtacgtaaa aaagtacgta aaaccgtgag
    12661 tgagggtatt gtgcacgttc atgcatcttt caacaatacc atcattacaa tcactgaccg
    12721 tcaaggcaat gcgttgtctt gggctacctc tggcggcgct ggttttaaag gttctcgtaa
    12781 aagtacacca tttgcagcac aagttgcagc agaagcagct ggtaaagttg cccaagagta
    12841 tggcgttaaa aatttagagg ttcgtattaa aggtccaggt ccaggtcgtg aatcctctgt
    12901 acgtgctttg aatgctcttg gtttcaagat taccagcatt actgacgtta ccccgttgcc
    12961 tcataacggt tgccgtccgc ctaaaaaacg tcgtatttaa tattggagtg atttgaaaca
    13021 tggcacgtta tattggccct aaatgtaagt tggcacgtcg cgaaggtacg gatttgtttt
    13081 tgaagagtgc gcgccgctct ttggattcta aatgtaaaat tgattccgct cccggtcagc
    13141 atggtgcaaa aaaaccgcgt ttgtcagact atggtttgca gttgcgtgaa aaacaaaaaa
    13201 tccgccgtat ttatggcgta ttagaacgtc agttccgtcg ttatttcgca gaagctgatc
    13261 gtcgtaaagg ttctaccggc gagttgctgt tgcagttgct ggaatctcgt ttggataatg
    13321 tcgtttatcg tatgggtttc ggttctaccc gagctgaagc aagacagctt gtttctcata
    13381 aggcgatagt tgtgaatgga caagttgtca atattccttc tttccaagtg aaagctggtg
    13441 atgttgtctc agttcgtgaa aaagccaaaa aacaggtacg tattcaagaa gcattgggtt
    13501 tggcaactca aatcggcttg ccgggttggg tttctgtaga tgcggataaa cttgagggtg
    13561 tgttcaaaaa catgccggat cgctcggaat tgaccggtga tattaatgaa cagctggtgg
    13621 tagagttcta ctctaaataa tgctagctca gtgagggaca gttaaatgca gaatagcaca
    13681 accgaatttt tgaaacctcg tcaaattgat gtaaatactt tttctgcaac tcgtgcaaaa
    13741 gtatctatgc agccatttga acgtggtttc ggtcatacct taggtaatgc tttgcgccgt
    13801 atcttactgt catccatgaa tggttttgct cctactgaag tagctattgc cggtgtatta
    13861 cacgaatatt ctactgttga tggtattcag gaagatgttg ttgacatttt gctgaatatt
    13921 aaaggtattg tgtttaaact ccatggtcgt agccaagttc aacttgtgtt gaagaaatca
    13981 ggttcaggtg tcgtatctgc cggtgatatt gagttgccgc atgatgtaga aattctgaac
    14041 cctggtcatg tcatttgtca tttggctgat aacggtcaaa ttgagatgga aattaaagta
    14101 gagcaaggtc gtggttatca atctgtttca ggtcgtcagg tagttcgtga tgagaaccgt
    14161 cagattggtg caatccagtt ggatgcgagc ttttcgccca tcagccgtgt tagctttgag
    14221 gttgaacctg cacgtgtaga gcagcggacg gatcttgata agttggtttt ggatatcgaa
    14281 accgacggtt ctattgatcc tgaggaagct gtacgcagtg cggcacgtat tttgattgat
    14341 cagatgtcta tttttgctga tttgcagggt acgcctgtgg aggaggttga agaaaaagca
    14401 cctcctatcg accctgttct tttgcgtccg gtggatgatc tggaattgac agtacgttca
    14461 gctaattgtt tgaaagctga ggatatttat tatattggcg atttgattca acgcactgaa
    14521 accgagcttc ttaaaacgcc gaatttggga cgtaaatctt tgaatgagat taaggaagta
    14581 ttggcatcta aaggtttgac actgggttct aagttggaag catggccacc tgtaggcttg
    14641 gaaaagcctt aatgaagaat taaaggataa ttgatatgcg tcatcgtaat ggcaatcgca
    14701 aattaaaccg taccagcagt catcgtgctg caatgctgcg taatatggcg aattcattat
    14761 tgactcacga agctattgta acaactctgc ctaaggccaa ggaattgcgc cgtgtagtag
    14821 agccgttgat tacattgggt aaaaagccgt cattggcaaa ccgccgtttg gcatttgacc
    14881 gtactcgcga ccgtgatgtt gtagtaaaac tgtttggcga tttgggtcct cgttttactg
    14941 ctcgtaacgg tggttatgtt cgggtgttga aatacggatt ccgtaaaggt gataatgcac
    15001 ctctggcact ggttgaattg gttgacaaac cggctgctga gtaattttag tcatataacg
    15061 ccatctgccg aaaagcaggt ggcgttattt ttgcaatatc tgataggtaa tagggtattg
    15121 gctatcatgt ttaaaatatt aattgaatag ctaaggtttg cgcggtaaac ttacatcatt
    15181 aaaaaattct atgatggttt atataatgaa tgctttcgat ataaagtcga caaagatgga
    15241 cgtattgtct atatctttgc atacgtcaga cttgtttgat ttggaagatg tgctggtcaa
    15301 attgggcaag aagtttcaag agtctggtgt tgttccattt gtgctggatg ttcaagagtt
    15361 tgattatccc gagtctttgg atcttgctgc attggtttcg ttgttttcaa ggcatggtat
    15421 gcaaattttg ggtctgaagc attctaatga acgttgggct gctgcggcta tgaagtatca
    15481 tttgctgttt tgtctgtctc attcggaaaa tgttaaagaa ctgggtcagg ttgaggtgca
    15541 gaacacggag gatggtcaga aagcaaggaa aacagtattg attacatccc ctgtccgtac
    15601 cggtcagcag gtttatgccg aagatggcga tttgattgtt acgggggcgg tcagccaggg
    15661 ggcggaattg attgcggatg gcaatataca tatttatgcg ccgatgaggg ggcgtgcttt
    15721 ggccggtgcc aagggtgata cttctgcccg catatttatc cactccatgc aggcagaact
    15781 ggtttctgtg gcgggtattt accgtaattt tgaacaggat ttgccgaacc atctgcacaa
    15841 gcagccggta cagatattgt tgcaggataa ccgattggtt atcagtgcaa ttggctcaga
    15901 gtaattgttt gatattttaa aaaggaaata ttgtggcaaa aattattgta gtaacttcag
    15961 gtaagggcgg tgtcggtaaa acgactacca gtgccagtat tgcgacaggt ttggcattac
    16021 gcggatataa aactgcggta attgattttg atgtgggctt gcgtaacctc gacctcatta
    16081 tgggttgtga gcgtcgtgtc gtttatgacc tgatcaatgt cattcagggg gaggcgacgc
    16141 tcaaccaagc tttgattaaa gataaaaatt gtgaaaacct gtttattttg ccggcttccc
    16201 agactcggga taaagacgct ttgacacgcg agggcgtaga aaaagtgatg caggagctgt
    16261 ccggcaagaa aatgggcttt gagtatatta tttgcgactc tcctgccggt attgagcagg
    16321 gtgcattgat ggcgttgtat tttgctgatg aagccattgt aacgaccaat cctgaggttt
    16381 ccagtgtgcg tgactccgac aggattttgg gaattttgca aagcaaatcc cataaggcag
    16441 agcaaggcgg ttcggttaaa gaacatctgt tgattacgcg ttattctccc gaacgagtgg
    16501 caaaaggcga aatgctgtct gtacaggata tttgcgatat tctgcatatt cctttgctgg
    16561 gtgtgattcc tgaatcccaa aacgtcttgc aggcatccaa ttccggagaa ccggtcatcc
    16621 atcaggacag cgtggcggct tccgaggcat ataaggacgt tattgcccgt cttttgggcg
    16681 agaaccgtga aatgcgtttc ttggaagctg agaaaaaaag cttcttcaaa cgtctgtttg
    16741 gaggataagg tatgtcatta atcgaatttt tattcggcag aaagcagaaa acggcaaccg
    16801 ttgcccgcga ccgccttcaa atcatcattg cccaagagcg cgcccaagaa ggtcaggctc
    16861 cggattacct gccgacttta cgtaaagagt tgatggaagt cctgtccaaa tatgtgaatg
    16921 tttcattaga caatatccgt atttcccagg aaaaacagga tggtatggat gtgcttgagt
    16981 tgaacattac tttgccggaa cagaaaaagg tataggacat gaccttaacc gaattgcggt
    17041 acatcgtcgc agtcgcccaa gaacgtcatt tcggcagggc ggcgcggcgt tgttttgtca
    17101 gtcagcccac tttgtctatt gccattaaga aattggaaga agagcttgcc gtctctttgt
    17161 ttgaccggag cagcaacgat attattacga ccgaggcggg ggaacgtatc gttgcacagg
    17221 cgcgtaaggt gttggaagag gcagagctta tcaggcattt ggcaaatgaa gaacaaaacg
    17281 agctggaggg tgcgttcaaa ctcgggctga tttttacggt tgcgccgtac ctgctgccga
    17341 aactgattgt ctcgttgcgc cgtactgcac cgaaaatgcc tttgatgttg gaagagaatt
    17401 acacgcatac tttgaccgag tcgctcaaac gcggggacgt tgatgcgatt atcgttgccg
    17461 aaccgtttca agagccgggc attgttaccg aacccttgta tgacgaaccg tttttcgtga
    17521 ttgtcccgaa agggcattca tttgaggaac tggatgccgt ttcgccccgg atgctgggtg
    17581 aggagcaggt tttgctgctg acggaaggca actgtatgcg ggatcaggta ctctcaagct
    17641 gttccgaatt ggcggcgaaa caacgtatac aggggttgac caatacattg cagggcagct
    17701 cgattaatac aatccgccat atggttgcca gcggtttggc aatcagcgtg ttgccggcaa
    17761 ccgcactgac cgaaaacgat catatgctgt tcagcattat tccgtttgaa ggtacgccgc
    17821 caagccggcg ggtcgtattg gcgtaccgcc gcaattttgt ccgtccgaag gcgttgtcgg
    17881 cgatgaaggc ggcgattatg cagtcgcagc ttcacggggt aagttttatc cgcgactagg
    17941 cgcaggcatt gttttcaaaa cgccatttcc ctgagccgac aacacggtat gccaagatat
    18001 tgccgtcatc atcgattttg agtatagcat cgccacggaa actgccgtcc tgaagatatt
    18061 cgacttttgc atcactgtga atgttttcat cagtgccgat gcaatgccat gtatagtgga
    18121 ttaacaaaaa ccagtacggc gttgcctcac cttgccgtac tatttgtact gtctgcggct
    18181 tcgtcgcctt gtcctgattt ttgttaatcc actataaaag aggccgtctg aaaaacattt
    18241 ttcagacggc cttgtttatt caatcaaatc agtctttcaa cttcgccaac tgattttgaa
    18301 cttttgccat tttgtcttcc aattccgcca aatcggcttt gtctttttcc accagatgcg
    18361 ccggggcttt ttcggtgtag ccgggtttgg agagtttggc gttgagtttg tccaaggctt
    18421 tttgcagctt ctcggcttct ttgctcaaac gggcggtttc ggcggctttg tcgatttcga
    18481 ctttcaacat caggcgcgcg ccgttgcaga cggcgacggg cgcgtcttcg ctttcgggta
    18541 gggcggcgac ttgctgtgct tcggtcaggc gggtcatcat cggcaggtat ttgaggtagt
    18601 ccgccaagtc gtccgtgctt tcgacaaaca gcggggcttt cacgttgggc tggatgccca
    18661 tttcgccgcg caggttgcgg actgcgccga tcaaatcctg caacacggtc atttgcccga
    18721 atgccgtctg aacaatctcg ccgccgtcgg cttcggggaa gcgggcgagc atgatgctgt
    18781 cggcggtttt cgcgtcgcac ataggggcga cggtttgcca cagttcttcg gtgatgaacg
    18841 ggataatcgg gtgcagcagg cgcagggcgg cttcgagtac gcgcaataag gtatggcgtg
    18901 tggcgcgttg gcggctggcg cagccggttt gaagctgcac tttggcaagt tccaaatacc
    18961 agtcgcaata gtcgttccac acgaagctgt acagggtttc cgccgccaaa tcaaagcggt
    19021 aggtttcgta ggcttgcgtt acttgctcga tggtctgatt cagacggtct acaatccaca
    19081 tatcggggaa ggaatagccg cgcggttcgg tggcggttgc gccgtagccg cagtcttggt
    19141 tttcggtgtt catcaagacg aagttggtgg cgttccagat tttgttgcag aagttgcggt
    19201 agccttcggc gcgtttgaag tcgaagttca cgctgcgccc caagctggcg tagctcgcca
    19261 tggtgaaacg caatgcgtcc gcgcccatac tcggaatgcc ttcgggaaag agttttttgg
    19321 tggcttcttc cactttcggc gcggtttcag gtttgcgcag gccggtggta cgttttacca
    19381 gcagcttgtc caagccgatg ccgtcaatca aatccacagg atcgatgacg ttgccttcgg
    19441 atttggacat ttttttgcct tcgtggtcgc gcacgatgcc gtggatgtac acgtctttaa
    19501 acggtacttt gccggtaaag tgggtggtca tcataatcat gcgcgccacc cagaagaaga
    19561 tgatttcgta gccggttacc aagacgttgg acggcaggaa ggctttgagt tcgtcggttt
    19621 cagacggcca gccgagcgtg gaaaacggca cgagcgcgga ggagaaccat gtgtccaata
    19681 cgtcttcttc tcgggtcaag cctgttttgc cggcttgttt ttcggcttcc acctgattgc
    19741 gggcgacgta aacattgcct gcttcgtcgt accacgcggg gatttgatgc ccccaccaca
    19801 gttggcgcga gatgcaccag tcttggatgt tgttcatcca ttggttgtag gtgttgaccc
    19861 agttttcagg gataaagcgt accgcgccgc tgtcgacggc ttttttggct ttgtcggcga
    19921 ggctcaagcc tttgaattcg ctgtcaggct cgccgccgtt tggcgtggcg gacatggcga
    19981 caaaccattg gctggtcagc atcggctcaa tcaccgaacc ggtacggtcg cctttcggcg
    20041 tcatcagcgt gtgcgctttg atttcgacca agaaaccttg ttcctgcaaa tcggcaacca
    20101 tttgtttgcg cgcggcaaag cggtccaaac ctgcgtattt ttcaggcagg gcaaagcctt
    20161 gttgcgcttc gcctttgaag ttgaacactt cggcgtttgc cagcactttg gcttccaaat
    20221 cgaacacatt aatcaggcgc gtatcgtggc gtttgccgac ttcgtagtcg ttaaagtcgt
    20281 gcgcaggcgt gattttcacg cagccggtac caaagtcttt ttcaacgtat tcgtcggcga
    20341 taaccggaat ggtgcggccg gtcagcggca ggattaattc tttgccgatt aagtgggtat
    20401 aacgttcgtc ttcgggattg acggcaacgg caacgtcgcc caacagcgtt tcgggacggg
    20461 tggtggcgac gatgacggct tcggcaggat tgtccgccag cggatagcgg atgtgccaca
    20521 tagagccttg ttcttccacg ctttccactt ccaaatcaga cactgcggta cccaataccg
    20581 gatcccagtt caccaagcgt ttgccgcgat aaatcaagcc ctgttcatac aggcgtacga
    20641 acacttcggt tacggtttcg gcgcgtacat cgtccattgt gaaatactcg cgcgtccagt
    20701 cggcagaaca gcccacacgg cgcatctgct gcgtaatcgt gccgccggaa acttctttcc
    20761 attcccacac tttttccaag aatttttcac gacccaagtc atgacgggac acgttttgcg
    20821 cggcaagctg acgctcgacc acgatttgcg tggcgatgcc cgcgtggtcg gtgccgggaa
    20881 tccaggcggt gttgcagcct ttcatacggt agtagcgggt caggccgtcc ataatggttt
    20941 ggttgaaggc atgacccatg tgcagcgtgc cggttacatt gggcggcggc agttggatgg
    21001 aaaaagacgg tttggtcaaa tccatatcag gttggaaata gccctgctct tcccagtttt
    21061 gataatgttt ggattcgatt tcggctggat tgtatttgtc taacatgatg gaactttgtg
    21121 aaattaaggt tatttttgat gtgcggatta taacgcaaaa aggccgtctg aatcatttca
    21181 gacggccttt ggcatacagg ttttaaaaat ggaacaatac caggctgacg gcaatcaccg
    21241 ccatgcccat tgtcaggccg taaacggttt catggccgtc tgaatagcgt ttggcagccg
    21301 gcagcagctc gtccaacgcc aaaaacacca tcacaccggc tatcacgccg aataccgaac
    21361 caaacacggc aggcgacaaa aacggctgca aaaccaaata gcccaaagcc gcccccaacg
    21421 gctcggccaa gccggatagc agacacgccc acaccgtttt cttacggctg cgggtggcaa
    21481 aataaaccgg cgcggcgatg gaaatgccct ccggaatatt atggatggca atcgccaagg
    21541 ccaaaggcat cccgactgct ggattttcca atgtggcaaa aaacgtcgcc aagccttcgg
    21601 ggaaattgtg cgcagtaatc gcaaacgccg ccatcatgcc gactcgcgcg atatggcggc
    21661 gtttgctttc ttgaaacgac gggtcttgcg cgtctaaagt ttcatgcggg ttcggcacca
    21721 gacggtcaat cagcgcaatg ccgcccatcc ctgccaaaaa tgccatggtc gccgccgcaa
    21781 acgcgtggtc tttatcataa atttcagcga acgcctcgct ggacttactg aaaatctccg
    21841 tcagggaaac atacaccatc gcaccgccgg caaatgccaa accaaacgac aacacgcgcg
    21901 gattgggcgt tttggaaaac atcaccaagc cgctgcctaa tacggtaaac aaaccggcag
    21961 ccaatgtgat ggaaaaggca acggccaaat tggacatcga aaaatcgggc atgagaaaac
    22021 ctgcgctaaa agctgggaca ggttcagact aacacttttt aatgtatatg ataatagtta
    22081 ttatttattt tattgattgg atacacggat tttgaaacaa aagaccgtct gaaaaatgat
    22141 tttcagacgg cctttaaatt tgaaatgccg ctaaacctta gtgctttcca gcttaagcct
    22201 gataacgcga caggctcaaa tcgtcgctgc ggatttcggt gtctttgccg ctcacgatat
    22261 cggcggttaa ttttgccgaa cccagcgaca tggtccagcc taaagtaccg tggccggtat
    22321 tcagaaacag gttgtcaaag cgggtgcgac cgattaacgg cgtgctgtcg ggcgtcatcg
    22381 gcctgaggcc gctccagaac gatgcttggc tcaaatcgcc gccttccggg aacaagtcgt
    22441 tgacgaccaa agccaaggtt tcgcggcgtt tttcgggcag tttgatttcg tagcccgaca
    22501 attccgccat accgccgacg cggattctgt tgtcaaagcg cgtgatggcg actttgtagc
    22561 tttcatctaa aacggtggac accggtgcgc cgtcggaatt tgtgaccggc agggtcaagg
    22621 aatagccttt gacgggataa atgggcagat tgagatccaa ctgcgccaaa accgtcctgc
    22681 tgaaacaacc gagcgcgcag acaacggcat ctgcttcaaa ccgccctgtt tcggtttcaa
    22741 cggttttgat gcgcagcccg ttgtggtcga tgcggctgat ggtttggttg aaatgaaacc
    22801 gtacgccctt ttcctgacac aatttgtata ggttttcagt gaagaggcgg cagtcgccgg
    22861 ttgcatctgc aggcaggtgc aggccgctgg caattttggc ggtaacgcgt gccagcgcag
    22921 gctcaaattc tgcacattct tcgggtttca gacggcggta cggcacgccg tagcgttcca
    22981 aaacggcaat gtcttgttct gccgcttcga cttctttggt ttggcggaaa atctgcaacg
    23041 tccctttttt gcgttcctca aaattcatgc cggtttgcgc ttcaaaacgg cggaacattt
    23101 cacggctgta ttcggaaatc ctgaccatgc gctctttatt ggtttgatag tgcgctgccg
    23161 tgcagttttg cagcatttgc cacagccatt cgatttgata caggctgccg tcggggcgaa
    23221 acagcaaagg cggatggctt ttaaacagcc atttcagcgc tttggtcggg ataccgggtg
    23281 cagcccaagg cgtggtatag ccgtaagaaa gctggcctgc gttggcaaaa ctggtttcca
    23341 tcgccacacc ctcggcgcgg tcgatgaccg ttacttcatg tcctgcctct gccagatacc
    23401 acgcggaaga cacgccggca acacccgcac ctaaaacaag cactttcatg tttctccctc
    23461 cggctttttc aaaacagact taatatgccg tgccgtctga atattcggat tcagacggcc
    23521 tcggatatta atgcggcaat tcgccgtttg tgattttttg tttgaagtcg cgcgtttcat
    23581 tgacgatgac tttcgccatc aataaaagtg caatcaggtt gggcaatgcc atcaagccgt
    23641 tgaatgtgtc cgaagccagc cacaccaaat caaggctcaa cacggtacct agcataacgg
    23701 aagaaacata accgacgcgg tacaaaccgg caaatttctc gccgaaaaca tacaccgcgc
    23761 atttttcgcc gtaatagcac cagcccaaaa tggttgagta ggcaaagaaa atcaggccga
    23821 tggtgacaat ccagccgccg atgccgggca gcattttttg gaatgtgacg gttgtcagtg
    23881 ccgcgccgct cacttcaggt ttgacaaact cgccgcccgc gccgagcagt cccattacca
    23941 acacgatgcc ggtaatcgag caaacgacga tggtatccaa aaacgtaccg gtcatagaaa
    24001 ccaaggcctg acggacggga tggtcggttt tcgcggctgc ggcggcaata ggcgcagaac
    24061 ccatacctgc ctcattggag aacacgccgc gcgccacgcc gtagcggatg accgtaccga
    24121 tagcaccgcc cgccactgcc tgcgcgctga acgcatcgga gaaaatcagc ttgacggcag
    24181 gcatcagtgc atcggaatta atcgcgataa tggaaagacc gcccaacaca taaaacaccg
    24241 ccatagcagg cacgatgaaa gaagcggctt tggcgatgcc tttaatacca cctaaaacga
    24301 caacggcagt cagaacggtc aacgtaatgc cggtataggc aggttcgata ccgaagctgg
    24361 tttgcaccgc ctgtgcaacc gagttggact gcaccgagct gccgataccg aaggaagcga
    24421 atgtgccgaa cagcgcaaat gcaacggcca tccatttcca gtttttgccc aagccttttt
    24481 cgatgtaata catcgggccg ccggacattt cgcctttgga attggtgacg cggtatttca
    24541 ccgccaacac gccctcgccg tatttggtgg ccatgccgaa aatggcggtc atccacatcc
    24601 aaaataccgc gcccgggccg ccggttacca ccgcagtcgc cacaccggcg atgttgcccg
    24661 taccgatagt ggcggacagc gcggtcatca acgccgcgaa atgggaaata tcgccttcgt
    24721 ggtcttcgcc gtcttcatgc ttctttgacg gcacaaacgc ctgtttcagt gcataaccca
    24781 acatagtgaa ctgcaagcct ttcagcaaaa cggtcagcaa aatgcccgta ccgaccagca
    24841 ataccaacat gacaggtccc cagacccagc cgctgatggt ttcaaaaaag gctttgagat
    24901 tgtctaaaaa caattgcatg gctttctcct ttgtctgttt tatttttaaa acaccacttt
    24961 tgtagtgtcc tgtaatttca gcacagaata ttcaataaga caatatgttc ttttgaaaaa
    25021 tacttttggt tttttcgccg aaagcaggtc ggttcaagct gcggaaattg tttgcaattc
    25081 tttaaaagca gcggcggagg tcacaatgaa atgtccgaat ggggatgtgg ccggcggcag
    25141 aaatcatcaa tgctgccgac tgccatactt ctgaaatcta caaaatgatg catcgatcaa
    25201 acaatatacc gctttaaaaa aaccgatgcc gtctgaaacg ctttcggggt ttcagacggc
    25261 atcaaaaggg tacggtcagc ggatgatgcc gcgcgccgat tgtgcgaaaa agtctcggaa
    25321 tacggcaagc tcggcttggg tttcggcgcg gcggagaatg tctgccttgg cttcttcaaa
    25381 cggaatgccg cgatggtaga gggttttgta cacgtctttg acggcggaaa tctgctctgc
    25441 ggtaaaaccg ttgcggcgca tgccttcgct gttgagcccc gccggttcgg cgcggtagcc
    25501 cgatgccata aagtagggcg gtacgtcttt gtgtacgcct gcggcgaagg cggtcatggc
    25561 gtagtcgccg atgcggcaga attggaaaac cagcgtgtag ccgcccaaaa cgacgtagtc
    25621 gccgatggta acgtgtccgg caagcgaggc gttgttggcg aaaatggtgt ggttgccgac
    25681 cacgcagtcg tgcgcgaggt ggcagtacgc cataatccag ttgtcgtcgc cgatacgggt
    25741 ttcgccgatg ccagtaactg ttccgagatt gaacgtcgta aattcgcgga tggtgttgcc
    25801 gttgccgata atcagccggg tcggctcgtc gcggtatttt ttgtcctgcg gaatttcgcc
    25861 gaggctggca aattggaaaa tgcggttgtt ttcgccgatg ctggtgtggc cgttgatgac
    25921 ggcgtgcggg ccgatttcgg tattcgcgcc gatttggacg ttggggccga tgacggtgta
    25981 cgcgccgact ttgacgctgg agtcgagttc ggctttgggg tcgatgacgg cggtcgggtg
    26041 gatgagggtc atgtttttcc tttcctgtcg tgttgccgcg aagatgcgcg acggcaacag
    26101 gtcgtctgaa aactttcaga cgaccttttt ctgaacactc aaaccacgcg tttggcgcac
    26161 ataatcacgg cttcgacggc gacttgcccg tccactttgg caacggcgtt gaatttgccg
    26221 atgccgcgcc ggctggtcag cagctcgact tcaaagacga gttggtcgcc ggggatgact
    26281 tggcgtttga aacgggcttc gtctatgccg gcgaagaaga agaattcgtt ttctttgcgc
    26341 ccgccttcgc tcaaaatcgc caacgtgccg cacgcctgcg ccatcgcttc gatgatgagt
    26401 acgccgggca tcacgggcag gtcgggaaaa tggccttgga actggggttc gtttatggtg
    26461 acgtttttaa tcgcggtcag ggttttcatc ggctcgaagg cggtgatgcg gtcgagctgg
    26521 agaaacggat agcggtgggg gatgagtttt tggatgtctt tggcttcgat ggggagttgt
    26581 acgtccatgt ctgtcgtatt ccttgaataa agtcggtttg gttatttgct gtcttgaccg
    26641 gcatctgaaa gctgctgctc cagtgttttg agccgtttgt tcatttcgct taagcggtgg
    26701 atgtaaacag cgttgcgcgc ccattcttta tgggtggaca tcgggaagat gccggcgagg
    26761 tgtttgccgc tttcggtaat gctgtgggtg acggacgtgc cgccgccgat ggtggttttg
    26821 tcggcgattt cgatgtgtcc gaccgtaccg acgccgccgc cgatgatgca gtagctgcct
    26881 atggttacgc tgcctgagat gccggttttg gcggcgatga cggtgtgcga accgattttg
    26941 cagttgtgtc cgatttggac ttggttgtcg attttggtgc cgttgccgac ggtggtgtcg
    27001 ctcatcgcgc cgcggtcgat gttggtgttc gagccgattt ccacgtcgtc gcccagcgtt
    27061 accgcgccgg tttgcgggat tttaaaccat gaatcgccgg caaaagcgag tccgaaaccg
    27121 tccgcgccga tgaccgcgcc gctgtggatt tcgacgcgtc tgcccagtgt gcagccgtaa
    27181 taaacgacgg cgttgggatg caggacgact tcgtcgccca gtttgcaatc gtgttggacg
    27241 acggcgtttg ccaagatgcg gcagccttcg ccgagcacgg tgtttgcgcc gatgtaggcg
    27301 ttcgcgccga tttcgcagct tgcgggaacg gtggcactcg gttcgacgac ggcggtcgga
    27361 tggatgccgc cgcgcgcttt gacgacgggt gaaaacaggc gggcgacttt ggcgaaatag
    27421 agataggggt cgtcggcgac aatcaggttg cgcccttcaa atccgtctgc cgctttggcg
    27481 gaaacgatga ccgcgcccgc gctgctgtcg tggacttcgg ctttgtattt cggattggca
    27541 aggaagctga tgtgttccgc ctgcgcgtct gcgagcgggc gcacggcggt aacggaaatg
    27601 tcctcgccgc gccattcgcc gccgagctgt gcggtgattt gggacagggt gtaggtggcc
    27661 ggaatcatgg ttttcctgtt cgatatgccg tctgaaaggg tcagcgggcg ttcatttctt
    27721 taatgacgct gtcggtaacg tcgtattggg tgttgacgta aatcacgttc tgcaaaatga
    27781 catcgtaacc ttcctgtttg gcgattttga cgatgacgcg gttggcgttt tgctggaggg
    27841 aggcaaactc ttcgttgcgg cggaggttgt agtcttcttc aaactgcgcc tgttttttgc
    27901 ggaacgctgc gaccagcccg cgccattttt cttcggcttg cgcctttttt gcgtttctga
    27961 gtttgccttc ggcaagctgc ctttccaaat ccagaccttc gcgttgcagt ttttgcaatt
    28021 cgtcctgacg agcggaaaat tcgctgtcca gcgttttttg aatcttgcgc gcctgcttgg
    28081 attcgaggta gatgcgctcg gtgttgataa agccgatttt ttggaaggtg tcggcgtgcg
    28141 cgcctgcggt gcagcacaaa ccgatcagag ccgcggcaaa cgcgcgggtc aaacgggtca
    28201 tggtaaaact ccttcgaatg ttgccgcgaa atgccgtctg aagggcttca gacggcattt
    28261 gcgggattag aacgtcgtgc cgagttggaa ttggaagcgt tggatttcgt cttccggttt
    28321 tttcttcagc gggtaggcgt agctgaattt catcgggcct aaaggcgaga gccaggtaac
    28381 cgcgccgccg gcggaatagc gcaattcgtt ggtaaaggtg gatttatggg tattgccggc
    28441 gccgtaaatg ttttgaaccc tgccgccggt cgcggaactg ctgttgtcgt cgtaggtttt
    28501 gccgtcccac acgctgcctg cgtcggcaaa caggctcagg cggacggtgc gcgcgtcttt
    28561 cgcgccgggc atcgggaaga gcagctcggc ggagacgttg gcttttttgt tgccgccgta
    28621 gctgattttt tcgccgtatt cgtcatacac tttcggaccg agcgtgccgc tttcgtaacc
    28681 tcgtaccgaa cccaggccgc cgccgtagaa gttttcaaag aaggggattt ctttggtttt
    28741 gccgtagccg ttggcatagc cgatttcgcc gccaagcatc agtgtgaagg ttttgctcag
    28801 ggggaagaac caggtttggt tgtgggtggc ggagtagtat tgcagtttgc tgccgggcag
    28861 ggcgatttcg gcgttcacgc ccgtcaggta gccgcgcgtc ggccataacg cgctgtcggt
    28921 tttgttgcgc ccccagccga cggtaccttt gtacagccag cctttgaagc tgccgtctgt
    28981 gccgtcggtt ttgccgtatt tcttgataaa gtcggcatag tgtttgggcg ctttgttgta
    29041 ggtgttgacg gtcaggtgtt ctgccaccaa accgaaattc acgcggtcgt attcggtaac
    29101 aggcacgctc atgcggatgc ctgcgcctgc cgtggtggtt ttatattgtt tgatgctggt
    29161 cgatgctttg cgcgggtcga aggcttttcc gtaaacatcg tagcccaggc tgaccccgtc
    29221 tgccgtgaag tacgggtcgg taaacgacag cgagccgttg agcgtggttt tgcttcgtga
    29281 ggcgcgcagg gcggccgact tgcccgtacc gaacaggttg tcttgggaaa cgcctgcgga
    29341 catgaccagg ccggtatcct gtacccagcc cgcgctcaag tcgagcgagc cggtggaacg
    29401 ttcggtcagg ctcatgttca aatcgacttt gtcgggtgtg ccggcaagcg ggacggcatc
    29461 aaactgtacg ttgtcgaagt agcccaaaag ctcgacgcgc tctttggagc gttgcagctt
    29521 ggaggtgtcg taaggcgcgg attccatttg gcgcaattcg cggcgcacga cttcgtcgcg
    29581 ggttttgttg ttgccggtga tgtggatttc gttgacgtag attttccggc ccggttcgat
    29641 gtgcaggacg aaatcgacgg ttttggtttc ggcgtttggc agcggctgta cgctgatttc
    29701 gctgtatgcg tagcctgccg agcccatgcg gttctgaatc tcacccaaaa cggcggtcat
    29761 ctgctggcgt tcgtaccatt tgccgggctt catggtcagc agtttttcca gttcggcttt
    29821 ggggacttcg ttggtgtcgc cttcgatgga gactttgccc caacggaaac gttcgccttc
    29881 gtggacggtg attttgatgg tctgcttggt tttgtcttcg ttggtttgga tgtcggtatc
    29941 gaggatgcgg aaatcgaagt agccgttgtt ctggtagaag tcggttactt tttccatgtc
    30001 ttgggcaaat ttctgctcgt tgaattggtt gcttcgtgtc agccatgtcc aaatgccgcc
    30061 ttcggtcagc gacatctgcc gcatcagttt gcggtcggaa tagacttggt tgccttcaaa
    30121 ttcgatgtcg gtgattttgg cggatttgcc ctcgtcaatc gtgatgtcga tgtcgacgcg
    30181 gttgcgggcg agtttggtta ctttgggcgt gatttggata ttgagtttgc cgcgcccgag
    30241 gtattcttct ttcaggccgg cgactgcctg attgagtgtc gcctgattaa agtattgcga
    30301 ctgcgccagc ccgaacgatt cgagattttt cttaatggcg tcgttttgca gcatttttgc
    30361 gccggtgatg ttgagcgagc cgatggtggg acgttcgatg acggtcagca ggagctgtcc
    30421 gtccgcggat tcaacgcgta cgtcgtcgaa gaacccggtc gcgtacaggc ttttgatgat
    30481 ttccccaccg cggccgtcgt tgtaggcatc gccgattttt acgggcaggt agttgaatac
    30541 ggtactcggt tcggtacgct gcaagccttc gacgcggatg tcttggatgg tgaagtcggc
    30601 aaatgccaaa ggcgatatgc ccaacatcat cagtgcggaa gcaatctgtt tcagtttcat
    30661 cgtaagttcc ttgtggtgcg gaatgcggtt tcagacggca ttccgaaacg taaaatctaa
    30721 ccgagcagcc gggtaacgtc gttgaagaag gcgaccgcca tcatcagcat catgagggca
    30781 agcccgaagc gcaaaccgat gttttggacg cgttcgccca aaggtttacc gcgtatccat
    30841 tcggcagtat aaaacacgag gtggccgccg tccaaaacgg ggacgggcag caagttcagc
    30901 acgccgaggc tgatgctgac cagtgccaaa aattccaaat aactttgcaa gccgagttcg
    30961 gcggactgtc cggcaatatc ggcaatggtc agcggaccgg aaatatggct gacggaggcg
    31021 ttgccgctga ttagtttgcc gaaaaatttg agggttgtcc acgagtggga aacggttttt
    31081 tcccagccca tgccgaatgc gcggataaca gacggacggt agctgcggcg gatttgcgcg
    31141 tcccacgccc tgtccggctg cggacggagg ccgacgcgcc cgatcagggt gtggtcggac
    31201 tgttcgacag tatcggggcg gatgtcggcg gtatgggttt gtccggcgcg ttcgtaggtt
    31261 aaggcaattt tcctgcccgg gctttgacgg gtcaggtttg cccattcttg ccatgaggta
    31321 atgggtttgc cgtcggcggc agtcagcctg tcgcccggtt tcaggcctgc tttttcggcg
    31381 gggctgcctt tttccacgcc gccagcaacg gttgtgattt taaagggcat cagtccgatg
    31441 tagccttggt tttttgcgat tttaccggct tccggcgtgc ttgcggcatc gatggtgcgg
    31501 acggtttgcg cgcccgatgc cgtctgaacg ccgacggcga ctttgccggc ttcgaggttg
    31561 aggacgattt cagtttgcgc gctgccccaa tctgcaacgg gtgtgccgtt gacggattgt
    31621 attttgtcgc cgctttggaa gccggcgcgg gcggcaatgg tgtcgggttc gactgtgccg
    31681 acgtaggggc gcagttcggt tacgccgaag gaaaagctca gtccgtacag caaaaccgcc
    31741 agtgcgaggt tggtcagtgg gccggcggcg acgatggcga tgcgcttggc ggggtgttgt
    31801 ttgtcaaaag cgtagggtaa atcggcttct gatacttcgc cttcgcgcgt atcgaccatt
    31861 ttgacgtaac cgcccaacgg aatcggggcg aggcaccatt cggtgtcgcc gcgctttcgg
    31921 gtgaaaaacg gtttgccgaa gccgacggaa aagcgtacga ctttgacgcc gcacaatctg
    31981 gcaacgatgt agtgtccgaa ctcgtgcagg ctgaccaaaa tcaggatggc gaagataaaa
    32041 gctagaaggg tgtgcaaatg gttttccttt gataacggtg ttcggacggc atcagcgcag
    32101 tgtgccgata aatgcccgcg cttgtgcgcg tgtccgggca tcttgcgcca agagcccccc
    32161 tatgtcgcct atgccgtctg aaaagtcttg tgcgagacaa tgggcgacgg ttttggcaat
    32221 gtcggtaaac ttaatctgcc cgtccaaaaa ggcggcgacg gcggcttcgt tggcggcgtt
    32281 caatacgcag ggcgcggctc cgcctgcgtt catggcttca taggcgagct tcaggcaggg
    32341 gaagcggtca aagtcgggct tttggaaggt cagcgcggac aatgcgtcga aatccaggtc
    32401 gccgacaccc gaatcgatgc gctcgggcaa acccaaacaa taagcgatgg gcgttcgcat
    32461 atcgggattg cccagttgcg ccaacacgga gccgtcgcgg tagcgcacca tgctgtgtat
    32521 cacggattgc ggatggatga cgacttcgag tttgtcgggc ggacagttga acagccaatg
    32581 cgcttcaatc agctccaaac ctttgttcat catggtggcg gaatcgacgg agattttgcg
    32641 tcccatacgc caattggggt gtttgaccgc ttggtcgggc gtaatgctgt cgaacgtgtt
    32701 taaatcggcg gtcagaaacg ggccgccgga agcggtcagg ataatcgaag cgatgccgtg
    32761 ttcgttcaga cggccggcgt aatcgcgcgg caaaacttgg aaaacggcgt tgtgttcgct
    32821 gtcgacgggc agcactgccg cgccgtttgc acgggcggtt tccataaaca acgcgccgga
    32881 aaccaccagc gtctctttgt tcgccagata aatggttttg cctttttgcg ccgctgcgag
    32941 cgcggaaggc agccccaccg ccccgacgat ggcgcacatg acaccgctga cttcgtcggc
    33001 agaggcaacg tcaaccaatg cctgcgcgcc gtgtaaaacc tgcgtcgccg tgccgtcgcc
    33061 tttcaacaaa gcttcaagcc gggcggcgtg ttcggcatcg gcaacgacgg catattcggg
    33121 gcggaacgtc cggcattgca ccgccagttt ctcgacctgc ttatgccctg ccagcgcgaa
    33181 tacgcggaat ttttcggggt ggagggagac aacgtccagc gtgctttcgc ctatgctgcc
    33241 ggtactgcct aatatggtca ggacttgtgg tgtcataatg gggataactt tataccggat
    33301 gccgtctgaa gcgttttcag acggcataga atcaatttaa aaccgacatc atcgccgcat
    33361 agacgctgat aacggcaatc aggctgtcgg tacggtcgaa cacgccgccg tgtccggaca
    33421 gcaggttgct gctgtcttta atgcccgccg cgcgcttgag ccagctttcc aacaggtcgc
    33481 cgcatacgct gacaacggtc agcaccaaac cgattaacac ggtatcgaac cagcctgtat
    33541 cgaatgccag ccagccggca cttcgtacgg cggtcatgta cactgccacg caaaccgcgc
    33601 cgccgattgc accttcccag cttttgccgg ggctgattgc cggtgcgatt ttgtgtttgc
    33661 cgaacgcctt gccgctgaaa tacgcgcaaa tatcggcaac ccacaccaaa cccatcacgg
    33721 cgagcagcgg cagggcatca tcgggatgcg ggcgcaggga tacgagcgcg aaccaaaacg
    33781 gcatgaccag aagccagccg acggcataaa cctgccaacc gccgttgagc ctccatttga
    33841 atctcaacca taaaggcata acggcgagcc aaaatgccaa aacaacatac caaaccaaat
    33901 taggcagcat ccagccgccc gcataggcaa ccacgccgaa aaccaaggtt gcggcgaggt
    33961 aatggttggt tttaattttg cacaaaccgc ccatacgggc atattcccac aaggcaatca
    34021 gggcaatcag tccgcaaaat gcagcccaca accattgcgg cgcgtaaaac agcatgccca
    34081 gcatcagcgg cagcagccac atggcggtta ttacccgttg tttcagcata ttcagttcct
    34141 ttgctgttcg ataggcagtt gctcggaggt gcgtccgaac cgccgttcgc gtttttggaa
    34201 cgaagcgacg gcatcgtcca aagccttgcc gtcaaaatcg ggccacaaaa tatcggtgaa
    34261 atacagttcg gcataagcca tttgccagag taggaaattg ctgatgcgcg tttcgccgcc
    34321 ggtgcggatg aacaaatccg gctcgggcgc atcgcccagc atcaagtgtt tcgccagcgt
    34381 gtcttccgta atctcggata cgcctttggc aatcagtttg tttgccgcct gcaaaatgtc
    34441 ccatctgccg ccgtaatcgg cggcaatgct cagggtcaga ccggtattgt ttgccgtcaa
    34501 cgcctccgcc tcttcgatgc ccttcaggat ttgacggctg aagcgttcgc ggctgcccag
    34561 tattttcagg cgcatattgt tttcgtgcag gcggcgtacc tgtttttgca aagcctgtaa
    34621 aaacagcccc atcaggaacg aaacttcgtc ttcggggcgg cgccagtttt cggttgaaaa
    34681 ggcaaacacg gtcagatatt gcacacccag tttggcgcaa tgcttcacca tattttccaa
    34741 tgcgtccaaa ccgcgtttgt gtcccattat gcgcgggagg aaacgttttt tcgcccaacg
    34801 gccgttgccg tccataatca cggcgatatg cttgggaatg gcggtgtgtt ccaaaacggc
    34861 ctgcgtgctg cttttcatgt ctgcctttcg cggttcggca ttcaaatgcc gtctgaacgc
    34921 cgaaccgtgc aggttaaatt gccatcaaat cttcttcttt ggcagtcagg agtttgtcgg
    34981 cttcggtaat gtatttgtcg gtcagttttt gaaccgcttc ttcgccgcga cgtgcctcgt
    35041 cttcggaaat ttctttgtct ttgaggagtt ttttgatgtg gtcgttggca tcgcggcgca
    35101 cgttgcggat agagacgcgg ccttcttccg cttcgccgcg tacgacttta atcaggtctt
    35161 tgcggcgttc ttcggtcagc atgggcatcg gcacgcggat caggtcgccg acagctgccg
    35221 ggttcagccc caagtttgaa tcgcggatgg ctttctcgac tttgaccgcc atattgccct
    35281 caaacggttt cacgccgatg gtgcgcgcgt ccagaagcgt tacgttggca acttggctga
    35341 cggggaccat gctgccccag tattcgactt ccacttggtc gagcaggccg gtatgcgcgc
    35401 ggccggtacg cactttcgcc agattttctt tcagtacttc gaccgaacgc tgcattttgc
    35461 cttcggctgt tttttgaata tcgttgatca tattgttctt tcggtgggat aaggtgggcg
    35521 ggagaccgtc tgaacgcgtt tcaagccgtt cagacggcat aaagaccgtt aaccgcgaat
    35581 agtaccgtta ttcgggcata acgacaaggt aggcggattg gggatgccgt ctgaagcgac
    35641 aggcgtttca gacggcatcg tgtccgaccg tcagccgtgt tcccgtgttt caagcaggct
    35701 ttggcgcagg tgttggcgtt cgtgggcatc cagccatttg cggcgggtgc gttgcagcag
    35761 gatgacgagg gcggaaattt cctgacgcat attggtgctg agccagagga agccctgcca
    35821 ttggtagtgg aggtgttcgg cgagggcttc cagttcgggg ttgatagcgg tgtcgatgcg
    35881 gatgcggcgg gcgtgtctgc cgttgataag ggcgacggtt tgttgcaggt cggtttggag
    35941 cagtgtgaag tggcggtcga gcagccggat ttcgctgccg ttgagtttgg gagattgcag
    36001 cttggcggca gtggtcagga gcagctcggt ggtgttgaca attttacggt gggcgtgctg
    36061 catggcttcc atcatggcgg ggctgatgcg gctttcgccc gatgtggcgg cgaggtggct
    36121 gcggcttttg accatgcgtg cgttgatttg gcgcattttc gccatgttct cttcgaggcg
    36181 ttcgcgggtc atgcgcctgc cgttgctgat ttcggcaatc attttgctgc agtcggtcag
    36241 gttgtcggca agcatgaaac gccacatcag tgtggatttc agcggcagca gtttggcggc
    36301 ggcgatggcg atggccgcgc cgatgaggac gttcatcgcg cgcatcaggc cgctgtcgaa
    36361 ccattcgctg ccgttgtcgc cgatgagcat gcacatcgtc agccccgcca gcatagggac
    36421 gtagccgttt ttgccgaccg ccgcccagcc ggccagtgcg cttgccgtgc cgacggtgag
    36481 gtagaagagg aggttgccgt ggaaataatg ctggttcagc cataaaacgc ccaaacccgc
    36541 gcccagcccg atgaccgtgc cgagcatacg ttccaccgcc ttggagtaaa tcgccccttg
    36601 aaactggagc atgccgagga cgacgaagac ggtcatccct atccactcgc cgtgttggag
    36661 gtggagcagc cgggcggagg cggtggcgaa caggacggcc ccgccgagcc ggacggcgtg
    36721 gatgaggcgg cggtagcggt agcgttcgta ggagttgagc cagcggctga cgaggcggtt
    36781 gcgttgcgag gtgttcatat cggttgtgcc gtctgaagcg gaaatgtgaa aaagcacagg
    36841 cttcccgagg aagggagggt ctgtgcttgg tattggtgcc ggagaaggga atcgaacccc
    36901 cgaccttcgc gttacgaatg cgctgctcta ccgactgagc tacaccggcg ttttttcgtc
    36961 atgatatata tgaacggttg tttgtgcaac ttttcgggcg ggcggcaagg cagtgcgcg
//
LOCUS       DAWFIE010000011        35907 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWFIE010000011 DAWFIE010000000
VERSION     DAWFIE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35907
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.011"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(120..1034)
                     /locus_tag="WGQ71_000515"
     CDS             complement(120..1034)
                     /locus_tag="WGQ71_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0706579.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEYFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WGQ71_000516"
     CDS             1290..2759
                     /locus_tag="WGQ71_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ0706580.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WGQ71_000517"
     CDS             3011..3382
                     /locus_tag="WGQ71_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706581.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLAPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WGQ71_000518"
     CDS             3455..4864
                     /locus_tag="WGQ71_000518"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ0706582.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALEKVGGKYGFTSGKSTHPDRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDVAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4911..5705
                     /locus_tag="WGQ71_000519"
     CDS             4911..5705
                     /locus_tag="WGQ71_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706583.1"
                     /translation="MTVPMQETHFSILLDELAAKQEAAIAPHLLADGTKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAARIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5893..6669
                     /locus_tag="WGQ71_000520"
     CDS             5893..6669
                     /locus_tag="WGQ71_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0706584.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(7337..9808)
                     /locus_tag="WGQ71_000521"
     CDS             complement(7337..9808)
                     /locus_tag="WGQ71_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ0706585.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPIDGTVLEGASTVDESMLTGESLPVAKMPSEKVTAGTLNTQNPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(9805..10983)
                     /gene="hflX"
                     /locus_tag="WGQ71_000522"
     CDS             complement(9805..10983)
                     /gene="hflX"
                     /locus_tag="WGQ71_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ0706586.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLREIHAGDILCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAEYCATAPNTDETEMP
                     "
     gene            complement(10988..12268)
                     /locus_tag="WGQ71_000523"
     CDS             complement(10988..12268)
                     /locus_tag="WGQ71_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ0706587.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12421..13146)
                     /locus_tag="WGQ71_000524"
     CDS             complement(12421..13146)
                     /locus_tag="WGQ71_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ0706588.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13219..13896)
                     /gene="radC"
                     /locus_tag="WGQ71_000525"
     CDS             complement(13219..13896)
                     /gene="radC"
                     /locus_tag="WGQ71_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ0706589.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(14019..15368)
                     /gene="gshA"
                     /locus_tag="WGQ71_000526"
     CDS             complement(14019..15368)
                     /gene="gshA"
                     /locus_tag="WGQ71_000526"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ0706590.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNMFYLQNVYALGEILCSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     AAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15628..17037
                     /gene="leuC"
                     /locus_tag="WGQ71_000527"
     CDS             15628..17037
                     /gene="leuC"
                     /locus_tag="WGQ71_000527"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ0706591.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDILDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPKGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17134..17388
                     /locus_tag="WGQ71_000528"
     CDS             17134..17388
                     /locus_tag="WGQ71_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706592.1"
                     /translation="MNKLFITALSALALSACASTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17450..18091
                     /gene="leuD"
                     /locus_tag="WGQ71_000529"
     CDS             17450..18091
                     /gene="leuD"
                     /locus_tag="WGQ71_000529"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ0706593.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18261..19532
                     /locus_tag="WGQ71_000530"
     CDS             18261..19532
                     /locus_tag="WGQ71_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0706594.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLEALGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            19548..20287
                     /locus_tag="WGQ71_000531"
                     /pseudo
     CDS             19548..20287
                     /locus_tag="WGQ71_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
     gene            20321..21391
                     /gene="leuB"
                     /locus_tag="WGQ71_000532"
     CDS             20321..21391
                     /gene="leuB"
                     /locus_tag="WGQ71_000532"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ0706595.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDVGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAILYKELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRILENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREVFEEIGKEYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATVLSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTND
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            21554..22117
                     /locus_tag="WGQ71_000533"
     CDS             21554..22117
                     /locus_tag="WGQ71_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ0706596.1"
                     /translation="MKKIIIAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTSIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
     gene            22578..23975
                     /gene="aspA"
                     /locus_tag="WGQ71_000534"
     CDS             22578..23975
                     /gene="aspA"
                     /locus_tag="WGQ71_000534"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ0706597.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFTAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(24047..24922)
                     /locus_tag="WGQ71_000535"
     CDS             complement(24047..24922)
                     /locus_tag="WGQ71_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0706598.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRAESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(24924..25646)
                     /locus_tag="WGQ71_000536"
     CDS             complement(24924..25646)
                     /locus_tag="WGQ71_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ0706599.1"
                     /translation="MDKDLYAVLGVSPQAGANEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSRHAYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYATHTEYLIKTFWRTFWLYLLGALTALLGIGVLIIIATNVWYF
                     YRIIVGFIRFNSGRAVAPEKWI"
     gene            complement(25668..26147)
                     /gene="ybaK"
                     /locus_tag="WGQ71_000537"
     CDS             complement(25668..26147)
                     /gene="ybaK"
                     /locus_tag="WGQ71_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ0706600.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLDIIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            26222..26584
                     /locus_tag="WGQ71_000538"
     CDS             26222..26584
                     /locus_tag="WGQ71_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0706601.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKVTAVKKDAATA"
     gene            26619..27482
                     /locus_tag="WGQ71_000539"
     CDS             26619..27482
                     /locus_tag="WGQ71_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ0706602.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASDEPLAAQEPNLAFAAQQDSDGICSIALPHGGILRIAPKNTLPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEEAGIVLDSVQDSENFTALTVIKFSAAQKELTVPNGGIFKA
                     VHLFFKTENAE"
     gene            27575..28534
                     /gene="ttcA"
                     /locus_tag="WGQ71_000540"
     CDS             27575..28534
                     /gene="ttcA"
                     /locus_tag="WGQ71_000540"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ0706603.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQSLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
     gene            complement(28668..29222)
                     /locus_tag="WGQ71_000541"
     CDS             complement(28668..29222)
                     /locus_tag="WGQ71_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0706604.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGFTEDLVQKASDKAVANESGNGLKN
                     TAGTIAMARTADPDSATGQFFINLADNDSLNYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVAE"
     gene            complement(29287..30213)
                     /locus_tag="WGQ71_000542"
     CDS             complement(29287..30213)
                     /locus_tag="WGQ71_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ0706605.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTSGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            complement(30201..30677)
                     /locus_tag="WGQ71_000543"
     CDS             complement(30201..30677)
                     /locus_tag="WGQ71_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ0706606.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            complement(30748..31203)
                     /locus_tag="WGQ71_000544"
     CDS             complement(30748..31203)
                     /locus_tag="WGQ71_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ0706607.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            complement(31328..32443)
                     /locus_tag="WGQ71_000545"
     CDS             complement(31328..32443)
                     /locus_tag="WGQ71_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ0706608.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQAIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     TCGQALLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIIGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            complement(32557..33654)
                     /locus_tag="WGQ71_000546"
     CDS             complement(32557..33654)
                     /locus_tag="WGQ71_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ0706609.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALAQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSEGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKVGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIAVVQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            complement(33736..35319)
                     /locus_tag="WGQ71_000547"
     CDS             complement(33736..35319)
                     /locus_tag="WGQ71_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ0706610.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLKPAAAAKAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTIDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     ASFVSWMAEHTRERILALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDNIRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEAFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTDYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQAVIDAVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            complement(35467..35679)
                     /locus_tag="WGQ71_000548"
     CDS             complement(35467..35679)
                     /locus_tag="WGQ71_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ0706611.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
BASE COUNT         8694 a         9580 c         9084 g         8549 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa tatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccccgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagc
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatactc caacttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcggaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggcg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta ttaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc agcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccggg ggcggtaaca cattcggcag cttagacggc ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggtcg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgctga aaaacaggcg gaatgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccgaaag gatgaccggc aacgatgtga tgcagatgct
     3301 ggctccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatac cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcacccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 agcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg caatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggtgt gaatgtattt atgctgtcgc ccgatggcaa
     3961 aatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacgggaata tccacaatat
     4021 cgccgtgaag ggaatgtttg acgactgtca ggatattgtg aaggcggtgc agaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaattcg atcaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcaggcta tttcaacgcc acttcaagca atgacgaaac
     4201 cgtcagcttc tgcgtaccga gcggcaactt cggcaacgtt tgcgcgggac atattgccaa
     4261 acagatgggc ctgcctgtcc gccgcctgat tgttgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggcg cataccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccgtct atggacattt ccaaagcgtc caacttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtgggcggaa gtagcggcgg gcaaaggctt
     4501 tgacctgcgg tttgctttag aaaaagtcgg tggcaaatac ggttttacct ccggcaaatc
     4561 cacccacccc gaccgcctcg ccaccatcaa acaggtttac gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggtgaaac
     4681 ggtggtttgt ttggaaaccg cgttggcggc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgtc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcaacg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatgttcc gtatgggcgg cattccgtcc gaacttaaga ttaaaagaaa atgaccgtac
     4921 ctatgcagga aacccatttt tccatactgc tggacgaact ggcggcaaaa caggaggcag
     4981 ccatcgcccc ccacctgctt gccgacggaa ccaaggtatg gatacgcaaa gcaggcaggc
     5041 acaatgcgcg atggcgttac gcgctgctcg gtatggttgc ccgatatttg aaactggggg
     5101 tgttgaagcc ggttcccagc ctcggcggcg agcctgccat tgcaaccgaa tcaaaacgcc
     5161 tgtacgaatt gcgcgcggcg aggatagccg ttcccgaatt gctcgccgtc aggaaaaatg
     5221 cccttatgtt cggcaatttg gaaggcatcc cgctcgatac gcaaatccgc caagaagccg
     5281 aagccggaaa ggcggacgct tggcttgccg gtttggaagc cattgcacgc gtgcataaaa
     5341 aacagcagtt cctcagtcag gcgtttgcgc ggaatatgat gtcggacgga aaaaacatca
     5401 gctttctaga ttttgaagac gacccttccg aagtcctgac catagcgcaa tgccaagccc
     5461 gcgattggct ctgctacatc cattcgaccg cgctgatatt gaaaaacggc ggactgctgg
     5521 aagcggctgc ggaaaaatgg ggcggcgtat tgtcggatca gcctgccgaa atacaaaagc
     5581 tgattgccgc caccgtcaaa ccgatcctcc cgatacgcag gctggaacac ccacgctggg
     5641 gacgggacgc actcaggctg gcagcctcga tttccctgat ttcccttgcc gatatgccgc
     5701 cataagccgc cgcccgcccc gcgatgccgt ctgaagccct tcagacggca tataaccaac
     5761 gcgcatcaag cattgtcgca accatccgtt tcgtttagaa ttttggcttt atcccgaatc
     5821 gggcagacat ttccactctg ccgcacctcc cgaacaagca ctatccattt cttttactga
     5881 caggaaaccc aaatggcagc attcaatacc caaaaagtat tgtccgtaca ccactggaca
     5941 gacgcatatt ttacctttac ctgcacccgc gacgaatcgt tgcgctttga aaacggacag
     6001 ttcgttatgg tcggattgat ggtggacggc aagccgctga tgcgcgcata cagcgtcgcc
     6061 tccgccaact gggaagaaca cctcgaattt ttcagcatta aagtccaaga cggcccgctg
     6121 accagccgcc tgcaacacct caaagtcggc gacgacgtgt taatcagcaa aaaaccgacc
     6181 ggaactctgg ttgccggcga cctgaatccg ggcaaacacc tttacttgtt gagcaccggt
     6241 accggcatcg cccctttctt gagcatcacc aaagaccccg agatttacga gcaatttgaa
     6301 aaaatcatcc tcgtacacgg cgtgcgctac aaaaaagatt tggcgtacta cgaccgcttt
     6361 accaaagaat tgcccgaaca cgaatacctc ggcgacttgg ttaaagaaaa actgatttac
     6421 tacccgattg tttcccgaga agaattcgaa caccacggcc gtctgaccga cctgatggta
     6481 agcggcaaac tgtttgaaga catcggcctg cccaaaatca acccgcaaga cgaccgcgcg
     6541 atgctgtgcg gcagccccgc gatgctgaaa gatacctgca aagttttgga cgatttcggt
     6601 ctgaccgtct ctccgaaaac cggcgtgcgc ggcgattacc tgattgagcg cgcgtttgtg
     6661 gatcaataag ctgcttgaaa atgccgtctg aacggcatca gcgataaaaa aacccgaatc
     6721 ctgccaatca ggattcgggg tttttatagt gggtaaaaat tagaaatgca catttccgtc
     6781 attccaacac aggtgggaat ccggacttac ctgtttcatt atcgtttgaa aataaaagag
     6841 aaaccaaaca tatagattcc cgcttgcgcg ggactgacga cggtgttctg cgtagccaaa
     6901 gcagaaaaac atccttattg tcatttcatc atgatataac aataaggatg ttaaaaatta
     6961 gttatcatta tatgcatgat atataagaaa aattgtccca ataagaaatg ttatgcagaa
     7021 aataaaatga aaaggatggt tttcattgaa aatggttgtt ctgccatcgt catacacaga
     7081 accggtattt ggaaaaaaat aatccatatt gacatcatta ttccatttat cgcataaatc
     7141 cactttttaa tttttttcca cattaatata atctcttatt taataaaatc aaaataaaaa
     7201 tcaaatgcct aacaattcga tctcaaattt tgaacgccaa cgtgtttgag cggtaacaat
     7261 acatacttta agtttgcaat ccttcatcgt cttatttttg ttttcatcaa ctatacgtct
     7321 aaagcaacgc cgtccgtcat tgttcggacg gcattttttc agactgcatt ttcccccgtt
     7381 tgtgaaggcg cagggcgttg cccaaaaccg ccagcgaact gaagctcata cccagtgcgg
     7441 ctatccacgg ttggacatag cccaaaacgg caagcggtac ggcaatgata ttgtacgcgc
     7501 ccgcccatat caggttttgc cggataatat ggcgggtgcg ccgcgcctga tcgagcaggt
     7561 gggcgacggt acgcaaatct tcgttcaata acacaatgtc cgcgccgtcc ctcgcaatat
     7621 ccgtcccgcc cgctgcggcg gcggatacgt ctgcctgcgc caaaacgggc gcgtcgttga
     7681 tgccgtcgcc tatcatcagc acttttttcc cttctttttg caaggctttg acgtattcca
     7741 gtttgtcctc gggcatggct tgggcgcggt agtgcgcgac acccagggca cgggcggttt
     7801 cggcaacggc ggtttcgcgg tcgccgctga ggatgtgcag ggtcaggttt ttgcctgcca
     7861 actgccgcac cgcctccgcc gcgctgtctt tcaaggggtc ggtcaggtag aacacggctt
     7921 ggaaaccgct ttgactgccg aggtaaaccg cgctgccgcc gccttctgtt tgcggttctt
     7981 tacctgaaat ttcggcgaca taggatgccc tgcccaatgc ccaaacctgt gtttcgccgt
     8041 tgacggtcag ttgcgcgccc acgccttcgc cgatgcggtt gaggcgttgt ttaatagcga
     8101 tgtcggggac gctgccgtct gaaatgcggc agttgaggat ggcgcgggca aggggatgtt
     8161 cggactgttg ttctaaagcc tgcgccaccg cgagaacaaa ggcttcgtct gtgcctctca
     8221 acaatgagat acggcggacg gcgggtttgc cttgggtcag cgtgccggtt ttgtcgaaga
     8281 tgatgtcggt ggtttgggcg agggtttcga ttgcctgctt tccgccgatt aaaatacctt
     8341 cgcgcgccag cgtaccggta gaagctgcca gcgcggtcgg cgtggcaagc gataaggcgc
     8401 aggggcaggt aatgaccagc agggcgacgg taatccacaa tgcggtgtgc gcgtcggcgt
     8461 acagcgtcca gccgatgaag acggggacgg caagcaggag ttcgccgaat atgaaagacg
     8521 aggcgtattg ttccgccaac tcggcagtgc gcggtttttg cgctaaggcg cggtcgagca
     8581 ggcggacgat gtgcgacagt cgcgtgccgc cgccggtgcg gtcggtgcgt ataatcagag
     8641 ggttttgcgt gttgagtgta cctgcggtta ctttttcaga cggcattttg gcgacgggca
     8701 ggctctcgcc ggtcagcata gattcgtcaa ccgtgcttgc gccttccagc accgtgccgt
     8761 caatggggat ggtttcgccc ggtttgacca gcacgatatc gcccgccttc aacttgacga
     8821 cagctgcctc gcaggtttcc tgcgtatcgg ggtaatcggg catatgatgg caaaacgcag
     8881 gaatcagctt caccagcctc tcggcggcat cgcctgcctt acggcgggca atgtgttcca
     8941 taaagcgtcc gcccagcagg aaaaacagca gcatggctat ggattcgaaa tacatcccct
     9001 gccccgcatt ggtcgcaagg ctgtaaacgc cggcgataaa ggtcatgatg atggcgacgg
     9061 caatcggcgt atccatgccg gcgcggcggt ttttcaggtc gcgcaacgcg ccttgataaa
     9121 acgggacggc gcaatagaat acgacgggca gcaccattaa aaagccgccc caatggagga
     9181 tttgcaggaa atcgggttcg atgtcgccgc cgtaaaggta ggtcggcagc gcgaacatca
     9241 tcgtctgcat catccccagc ccggcaacgg cgaggcggac gatgtattgt ttgcgttctt
     9301 tttggttggc ggcttcgatt ttttgcgcgt catagggtgc ggcggtgtag cctatctgcc
     9361 tgattttcaa cagaatgtcg gaaaggcgga ttttgccgtc gtcccagacg acgcggcagc
     9421 ggtgcgtgct gtaattgagg tcgatgcgga cgatgccgtc tgtacgcaaa agctgctgtt
     9481 cgatcagcca gacgcaggcg gcgcaggtga tgccgccgag cattaaaacc gcctcgcgcg
     9541 tgccgccgtg ggtttccaca aagtcggact ggacttcggg caggtcgtac aggcggattt
     9601 ggtcgaggat ttcttggggc ggcagctcgg ttttttgcgc gtcggcggtg cgttgtttgt
     9661 aataactgcc caagcccgcg tcaataatgc tttgtgcgac cgcctgacaa ccggcgcagc
     9721 aggtttcgcg gtcttcattt tcgtaacgga cggtcagatg caggttttcg ggaacgtcca
     9781 gcccgcagtg gaaacaggtt tttttcatgg catttcggtt tcgtctgtgt ttggtgcggt
     9841 ggcacaatac tcggcaatgg cttcgcgcag tgcgtctata ccggtatttt cagcaacgga
     9901 aatgcggacg gcggcaattt ttcccgcagc gtcgcgccat atgcccgtgt tttgttcttc
     9961 agacggcagc aggtcggttt tgttgtacac cttgatgcac agaatatcgc cggcatggat
    10021 ttctcgcagt acgttttcca cgtcttcaat ctgctgtccg ctgttcggag cggcggcatc
    10081 gacgacgtgc agcagcacat cggcttgcgc ggtttcttcc agcgtggcgg aaaaggcgga
    10141 aatcagtttg tgcggcagat cgctgacgaa tccgacggta tcggtcagga tgatgctgca
    10201 ttcgggactg atgtacagcc gccgcgccgt ggtgtcgagt gtggcgaaaa gctggtcttt
    10261 cgcatatatg cccgacttgg tcagccggtt gaacaggctg gatttgccga cattggtata
    10321 gccgaccagc gcaaacgttt tgattgtgcc cgattcgcgg gacttgcggc gcagggcgcg
    10381 ctgttttttg aggttggcaa gctgtttttt caaggcgttg attcgatggg cgatcaatcg
    10441 gcggtcggtt tccagtttgg tttcgcccgg ccctttcatg ccgataccgc cgcgctggct
    10501 ctgcagatgg ccgtaaccgc gtatcaagcg tcccgccaaa tggctcaatt gcgccaactc
    10561 gacttgcagc ctgccttcct gcgtgcgggc gcggcgggcg aaaatcgcca gaatcagccc
    10621 caccctgtcc aatacgcggc acttcagctc cttttcgagg ttgcgctctt gcgtgggcgt
    10681 aagttcgtgg ttgaatacga ccaaatcgat gccgtctgcg gcaactgctt ccgacagctc
    10741 cgccgccttg cccgtgccga caaacagcgc ggtgtgcgga cggtcgcgtt tggcagtctc
    10801 cacgcgcacg gaatcgccgc ccgccgcctt gaccagctcg acagcttccg ccaatgccgt
    10861 ctgaaaaccg ttcagacggg cggcactgct gcccgtacca tctttgtcca acattacgcc
    10921 caccagcatg acgcgttccg gttgcgcttg ggtactgccg ttgcggcctg ttctgcctgt
    10981 catgtattca atcctgtaaa taaagggtta cgcctcgttc cgtttctcct tccggtgcaa
    11041 gcgtgatgcg gctgcccgcc attccgtcaa cgctgaagac gatgccgccc gcctcgtgcc
    11101 ttcctatgga caggcggctg ccgggtcgct gccggtaggt ataacgggta cacgcttcca
    11161 aacaccagcg tccgcgccag tggatgaaat atttttcgcc ttttctaacc gcaagtgtct
    11221 cgccgccgtt tacctcaaaa taagggtaat cgggttttcg ggttctaaac tgccatttcg
    11281 cctgcgcgcg ccgtccgttg cggacatagt cgaataccgc cgtataaagc gtaccgtatt
    11341 ccaaaggctt gagcgggaaa agcgcgaatt ggtacgcggt cagcctgccg ttggggtcgt
    11401 tgccggcggt taaaaccctg acggggcgga tttcgttttt accctgatac agcttgaaac
    11461 ttttcatcgt aattttgcct gccgcctcgg aaaaatcaat gctggcagga ttgcccgtga
    11521 tttcatattc cggcacgggg tctggacgct cgccgtggaa ataaggcagt gcgccgttgc
    11581 cgacgggata ggctgtatag agcagctcct gtgcgggcat ggcttcgtcg gtgtacacga
    11641 ccgcaccgtt atggcaggcg ttgcggtaat atttccgtcc tgcttccggc tgatttctgc
    11701 cttgggcgca atgccgctca aacctgccgt tgccctgatt gaatacgaga acggttttac
    11761 cgttttcgcg cacaaatgcc gctcctgcct catccgtatg gcggtcaagt agggaaaggc
    11821 ggtggtagat tgcgctcatt aatccgtcca cttggcgttg ctgcgtgcgg atgtcgctgt
    11881 cggacgattc ggcggcttcc tcttccgtgc tgatgttttc atgcacgccg ttgtagagat
    11941 acccggcaag gcgtgtgcgt tcggtcagct tttgtgcggt gtagtgcgga ttgtcgggat
    12001 ggtgttcgcc gtgtccgtct tcgggattga gcgtgagata gcgtgcgtgc ctgcgggcgg
    12061 aattttccaa aaccggcgcg tgtgccagct tgtgcaaacc gatttgggcg cggatgcggt
    12121 tgaggtagtg cagcgcatcg ccgccgtccc tgatttgcgg tgcggacgga tagacaagtt
    12181 cgcccgcggg cagggattgg ttttgggtat gatagaaaac gccggccgcc gaacccaaca
    12241 ggagcagccg aataaaaagg gatttcataa tgccgtcctc ccgacgcggc aacggggtgt
    12301 tttatcaaaa cggcaaactt gccaatgcgg tttccgattg cagattgcca aaccggcaaa
    12361 cctttccccg tgccgatgcc gaacctgatg ccgtctgaac cttcagacgg catcggatgt
    12421 ttatttgtcc acccggatgg gtgcaggttc gtattgtgtc gattcgtccc cgtaatacag
    12481 cacgccgagt ttgatgggga ttctgccctg cgatttgcgg tgggcattgg aatcgcgcaa
    12541 ggaataggcg caaccgcaat attcctgctg gtagaagttt tcacgtttgc tgatttcaat
    12601 catgcgcgcg ctgccgccgc ctttgcgcca gttgaaatcc caatacacca catcatcgta
    12661 aggcgcggcg gcgcggtgtc cgcagtcgtt gatttgcgcc atatttttcc agcgtgaaat
    12721 gcccagcgaa ctggtaaaga cggggaaccc gtgttcatgc gcgtattgcg ccgccttttc
    12781 aaaacgcata tcgaaacaca tggtgcagcg gatgccgcgt tccggctcaa actccatgcc
    12841 tttggctttg gcaaaccatt ctttgcggtc gttttcgtag tcgtcgtctt tatcgatgaa
    12901 aggaatgccg aacttttccg caaagcgcac gttttcctct tttcggagca tatactcttt
    12961 gtgcggatgg atattgggat tgtaaaaata aatagtgtaa tcaatgccgc tggccagcat
    13021 ggcttccatc acttcgccgc tgcacggggc gcaacaggaa tgcagcaaga cttttttatg
    13081 tccgccgggc gggacgagta tggggcggtc aatgtcggta acggtaggtt tgttttgtgt
    13141 ttccattaac ttaacgatat ctgtcgaata cggttgaagc ggctatttta tcagagccgc
    13201 cgcatgtaaa acagggtgtc agggcatcag cccgagctgc ctgaacgaac ggacggtttg
    13261 cgccgttacg ataaaatggt cgagcagcga cacatcgacc agcgacattg cctgtgccag
    13321 ccgccttgtg aacatgatgt cttcctgcga aggttcgggc gagccgcccg gatggttgtg
    13381 cgcaataatc aggctgtcgg catattcgtc caatgccagt ttgacgattt cgcggatgta
    13441 aatcgtgttt tccgcaaccg taccgcgcga cagctctctg accgcaatca gttggttttg
    13501 gcggttcagc agcagcgcga cgctgacttc gactttttcc tgccccaaat gaaagcgtaa
    13561 ataatcggcc accgtatccg gatcggacag gacgatgctc tcctgcaatt cttccgccaa
    13621 tatccgccgc ccgatttccc tgactacggc aaactgtgtg aaacttgccg tccccatccc
    13681 tttgtatgcc gacagtttgc cgacctccgc gctcatcagc ctccccaaac tgccgaactc
    13741 ctgcagcaaa taacgcgcca aatcgaccgc actcattccg cgcgtgccga cgcgcagcag
    13801 gattgccaaa agttcggcat cactcaaagc cgccgccccg cgttccaaca gcttttccct
    13861 gggcctttcc ccctccggcc attgcttgat gctcatgttt tccctttttt aaatagtctg
    13921 agatgccgtc tggaacaaat gttcccacac gccaaatatc cgaaatgccg tctgaaccgg
    13981 atttccgctt cagacggcat ctgtatttca aaacagtctt acgccgtttc ttccaactcg
    14041 atggacgccg ccagaagcga caggcgtgcc attacgccgt aaacgtagag gcggttgtgt
    14101 tcgttgtcca cgtcgcagtc tttttcagag cgcggcatac ccagcgagtc ccattgttcg
    14161 aatacgcgct tgcaggcttc gggagcttct tgggagttgt cgccgttgcc ggtggggatg
    14221 ctgttggaca gcggaacaaa caccataccg ccggcgttga ggttttcgtc cgcaccgcgc
    14281 ccttcgtgta cgcggaaaaa gccgccgatg acgaagcggt ccatcatata cacgacgggt
    14341 tcgcacaccg cgccgtttaa ggtttcataa gtataaatcc cttcttggac aatcacttcg
    14401 ctgacttcca aaccttcttt gactttcgcc attttattgc ggtttttacg gttcaatccg
    14461 cgcacttcgt cggcggattt gacgctcatc acgcccatgc cgtaagtgcc ggcatcggct
    14521 ttgacgatga cgaaaggttt gtcggtaatg cccgattcgt cgtatttggc ttgaattttc
    14581 gccagcacac gttctaccgc ctccgccaac gcgtcttcgc cttcacgccc ttggaagtcc
    14641 aaaccgccga ttttttcaaa ataagggtta atttgccatt cgtcgatgcc gattaacttg
    14701 gcaaattcgg cggcaacttg gttgtacgcg ccgaaatgat ttgttttgcg gcgcgtcgtc
    14761 caaccgccgt gcaacggcgg caaaacggtt tggctgatgc ctttgaggat gtcgggaata
    14821 ccggcggaca aatcgttgtt caacaaaacc acgcaaggcg aaaagccgtc tgcaagatgg
    14881 acgcggtcgc gggtacgcaa taaaggttcc aacaggattt tgtcgcccaa tgcggtttca
    14941 aattcggtcg gttcggttac ttccggattc aggctgccca agcgcacttc ataccctgcc
    15001 gaacacaaaa tctcgccgag ggcgtaaacg ttttgcaggt aaaacatatt gcgcgtgtgg
    15061 ttttcgggaa taatcaatac ggatttcgcc gtttcacagg cgcgttgcac cgcatcttgc
    15121 gcggcaaccg ccgccagcgg gataaagttg ggattcagat tattgaagcc gccggggaac
    15181 aaattcatat cgatagacga aattttgtaa ccggcattgc gtatatcgac cgaaccgtaa
    15241 aacggcgggc ggtgtacgct ccactgcgtg cggaaccacg cctcgatttt ggcgtgattg
    15301 gacaggattt ttgcctcaaa cgcctgaagt tgcgccgaat gttcgggcga catcaccggt
    15361 aatttcatct ttcgtctgcc tctgtgtgtt ttttgacaag atgcagatga taatgccttt
    15421 gaaagaatga cgacaagtat tctttcgtat cgaatcatgc tttagatata actgtttact
    15481 gaaatggaca atatgtctaa ttttttcgtt aaaattgttt gcaaaataac accggctcaa
    15541 aaattagaca aaatttgttg cgcggtataa agaatacgtc taaaatccgc ccaatccgca
    15601 ttaccattta tccaaaagaa caacatcatg acagcacaaa ccctctacga caaactttgg
    15661 aacagccacg tcgtccgcga agaagaagac ggcaccgtcc tgctctacat cgaccgccat
    15721 ttggtgcacg aagttaccag ccctcaggca tttgaaggct tgaaaatggc ggggcgcaag
    15781 ctgtggcgca tcgacagcgt cgtctccacc gccgaccaca acaccccgac cggcgattgg
    15841 gacaaaggca tccaagaccc gatttccaag ctgcaagtcg atattttgga caaaaacatt
    15901 aaagagtttg gcgcactcgc ctattttccg tttatggaca aaggtcaggg catcgtacac
    15961 gttatgggcc ccgaacaagg cgcgaccctg cccggtatga ccgtcgtctg cggcgactcg
    16021 cacacttcca cccacggcgc attcggcgca ctggcacacg gcatcggcac ttccgaagtc
    16081 gagcacacca tggcgaccca atgtattacc gcgaaaaaat ccaaatccat gctgattgcc
    16141 gttgacggca aattaaaagc gggcgttacc gccaaagacg tggcacttta catcatcggg
    16201 caaatcggca cggcgggcgg cacgggttac gccatcgagt ttggcggcga agccatccgc
    16261 agcctttcta tggaaggccg catgacttta tgcaatatgg cgatcgaagc cggtgcgcgc
    16321 tcaggcatgg ttgccgtcga ccaaaccacc atcgactatg taaaagataa acccttcgca
    16381 cctaaaggcg aagcatggga caaagccgtc gagtactggc gcacgctggt gtctgacgaa
    16441 ggtgcggtat tcgacaaaga ataccgtttc aacgccgaag acatcgaacc gcaagtcact
    16501 tggggtacct cgcctgaaat ggttttagac atcagcagca aagtgccgaa tcctgccgaa
    16561 gaaaccgatc cggtcaaacg cagcggtatg gaacgcgccc ttgaatacat gggcttggaa
    16621 gccggtacgc cattaaacga aatccccgtc gacatcgtat tcatcggctc ttgcaccaac
    16681 agccgcatcg aagacttgcg cgaagccgcc gccatcgcca aagaccgcaa aaaagccgcc
    16741 aacgtacagc gcgtgttaat cgtccccggc tccggtttgg ttaaagaaca agccgaaaaa
    16801 gaaggcttgg acaaaatttt catcgaagcc ggttttgaat ggcgcgaacc gggctgttcg
    16861 atgtgtctcg ccatgaacgc cgaccgcctg accccggggc aacgctgcgc ctccacctcc
    16921 aaccgcaatt ttgaaggccg tcaaggcaac ggcggacgta cccacctcgt cagccccgct
    16981 atggcagccg ccgccgccgt taccggccgc tttaccgaca tccgcatgat ggcgtaagta
    17041 ttttcaggcg gtctttaaga atttgacgcg gccgtctgaa atatgtcccg taaggcagaa
    17101 acacctttaa tattcaaacc aaggagttta accatgaaca aacttttcat taccgccctg
    17161 tccgccctcg ccttgtccgc ctgcgccagc acttgggagg gcgcgaaaca agacaccgcc
    17221 cgcaaccttg acaaaacaca ggccgccgcc gaacgcgccg ccgaacaaac aggcaacgcc
    17281 gtcgaaaaag gctgggacaa aaccaaagaa gccgtcaaaa aaggcggcaa tgccgtcgga
    17341 cgcggcattt cccatctcgg cggaaaaatc gaaaacgcca ccgaataacc gattcgcgat
    17401 gccgtctgaa accgcttcag acggcatcgt cagcacaaga cgcaaaccca tgaaagcctt
    17461 taccaaaatt accgccatcg tcgccccgct cgaccgcagc aacgtcgata ccgacgccat
    17521 catccccaaa caatttttaa aatccatcaa acgcagcggc ttcggcccca atgcctttga
    17581 cgaatggcgt tacctcgacc acggcgaacc aggcatggac aacagcaaac gcccgttgaa
    17641 ccctgatttc tcgctgaacc agccgcgtta ccaaggcgcg caaatcctgt tgacgcgtaa
    17701 aaacttcggt tgcggctctt cacgcgaaca cgccccttgg gcattggacg actacggctt
    17761 ccgcgccgtc atcgccccca gcttcgccga catcttcttc aacaactgct acaaaaacgg
    17821 ccttttacct atcgtgttga ccgaagaaca ggttgaccgg cttttcaaag aagttgaagc
    17881 caacgaaggc tatcagctct ccatcgacct tgccgaacaa accctgacca caccaagcgg
    17941 cgaaacattc acattcgaca ttaccgaaca ccgcaaacac tgcctcttaa acggcttgga
    18001 cgaaatcgga ctgaccctgc aacatgccga tgaaattcac gcctttgaag aaaaacgccg
    18061 ccaaagccag ccttggctgt ttaacggtta aaacctatac cgtctgaagg ccgttcagac
    18121 ggcattcctg ataacatata attgctgcaa ctaatgtaaa cagaggggcg ataaattgct
    18181 ttcatctttc aagcgggctt agcaacgctc taaattaatg caatacagct ctatttcaaa
    18241 taaattttta gatttttaat atgcaacaga ttaaatttat tgacttattt tccgggatga
    18301 gcggtatcag gaaaggattt gaacaagcct gtcggaaaca gtcggttgct tgcgaatgtg
    18361 tttttacctc agaaatcaaa ccggcagctt tggaagtatt gaagcaaaac tatcctgatg
    18421 aagtgccgta tggagatata acgaaaattg aaacgggaga tattcccgat tttgacatcc
    18481 tgttggcagg cttcccttgc caggcttttt ctttcgccgg aaaaagattg ggctttgaag
    18541 atacgcgggg aacgcttttc tttgatgtag caaggatttt aaaggcaaaa aaaccaaaag
    18601 gttttatttt ggaaaatgtg gaaggattgg taacgcacga tagaaaagat ccgacacaaa
    18661 aaatagggcg caccctgacc gttattttgg aaaccttgga agcattgggt tactatgttt
    18721 cttggaaagt tttaaatgca aaagatttcg gcattcccca aaacaggaag cgcatttatc
    18781 tgacaggcag tttgaaatcc aaacccgact tatcttttga aacaactcta agtccgaaat
    18841 taaaaaatat tttggaatcg ggactgccta ccgaaagcag tcctttcatc aaaaaattgc
    18901 taaaaaaatt tcccccgtcc gaactgtacg gaaaatcagt aaaggacaaa cggggaggga
    18961 aaaacaatat ccacagttgg gatattgaat taaaaggcac agtaaccgaa gaagagaagc
    19021 aattattaaa tatgcttcta aaagaacgga ggaagaaaaa atgggcttca gaaatcggca
    19081 tcgattggat ggatgggatg cctttgacaa aagcgcaaat ttcaactttc tataaacacc
    19141 ccaatcttca aaatattttg gacagcctga cagataaagg ctatttggtt ttggagcatc
    19201 cgaaacaaaa aattggcggg cgaagaatca aagatgaatc cctgcctaag ggctacaata
    19261 tcgtttcagg taaaaaatca tttgaaatca ataaaatatt agacccaaat gatgtcgcgc
    19321 caaccttagt tgcaatggat atggagcacc ttttcgtcgt tgacaacggc ggtttgcgta
    19381 cacttaccgg aaaagaaggt ttacgcttat tcggctatcc ggacgattat tcgtttgata
    19441 ttcctaaaaa agacaaatac gatttattgg gcaataccgt tgccgttcct gtgattaagg
    19501 cggtatctga aaggcttctg catactttat aaaccgatag ggataaaatg gtaaaactta
    19561 ctgcacaaca aatatttgat aagttgctga atgaggaaag aatcttatcg gctaatggtc
    19621 aaattagatt tttcttgggc gatgtggata ttatcgtcaa acaaaaagat gttgtcggca
    19681 acatcattca ggaatggctc ggcggatggt tgagaaaaag ggaaattgaa tttgatgttt
    19741 caaccaatac ccaaatgccc cccgatttct ttttaaataa aaaagaccgc agtagagaat
    19801 tgttggaagt aaaggcgttc aaccgaaatg ccagcccggg ttttgatatt gcagatttta
    19861 aaatgtattc tgatgaaatc attcataaac cctatatgct ggatgtagat tatttaatat
    19921 tcggttacga tatggacgac aacggcaatg taaccatcaa ggatttatgg cttaaaaaag
    19981 tatggcaaat taccagaagt atggatggat gggcaattaa tcttcaagtc aaaaaaggcg
    20041 tggtgcataa aatccgcccg ggtgtttggt acagcataaa taaaaagaat atgccaatgt
    20101 ttgaatgctt ggaagatttt gtttccgcaa ttgaagaaac cgtttatcaa aacccggcaa
    20161 cacggcaaca cggcataacg catccttatg gaaaaggaaa tttgaggaag cctataaaaa
    20221 acactacaac cgatcgattt ccataccccg ttggcacgaa attgcacata aataccaaaa
    20281 gaaataaccc ttcaaccatc aacccataag gaaacttaaa atgaccaaac atatcgccat
    20341 cctccgaggc gacggcatcg gccctgaaat cgtcgccgaa accgtccgcg tactcgacaa
    20401 actcatcgca caaggcttgg acgtcggcta cgaatacgcg cctttgggcg gcgaagccta
    20461 tgacgaatac ggccatcctt atccagaatt tacgcaaaac ctttgccgta aagccgatgc
    20521 cgtattactt ggtgcagtcg gttctccgca atacgacaat cttgaccgtc cgctgcgccc
    20581 tgagcgcggc ttgttggcca tccgtaaaga cctgaatctg tttgccaatt tgcgtcctgc
    20641 cattttgtat aaagagctgg ccaatgcttc cactttgaaa ccggaaatcg tagccggcct
    20701 cgacatcctt atcgtacgcg aactcaccgg tgatatttac tttggcgaac cgcgcggcat
    20761 ccgtattttg gaaaacggcg aacgcgaagg ctacaacacc atgaaataca gcgaaagcga
    20821 aatccgccgt attgcccatg ttgccttcca atccgcccaa aaacgcagca aaaaagtctg
    20881 ttccgtaggc aaagccaacg ttttggaaac caccgaactg tggcgcgaag tctttgaaga
    20941 aatcggcaaa gaataccctg atgtcgagct ttcccatatg tacgttgata acgcagccat
    21001 gcagttggta cgcgcgccta aacaatttga cgtgattgcc accggcaaca tattcggcga
    21061 cattctgtct gacgaagcct ccatgctgac cggttccatc ggtatgctgc cttccgcttc
    21121 tttggacgaa aacggcaaag gcctgtacga accgtctcac ggctctgctc ctgacattgc
    21181 cggtcaaaac aaagccaatc cgctggcgac cgtattgtct cttgctatgc tgctgcgtta
    21241 cagcctgaac gacgaagcac gcgcgcaaca agttgaaaac gctgtacaaa aagtactgca
    21301 acaaggcttg cgtaccaacg acatttacga agaaggcaca aaactcgttt cctgctccga
    21361 aatgggcgat gcggtactcg ccgccttgta aaaaaacaaa gcgcggatca caccgcgctt
    21421 tttcatctgc ccttttccgc tcgccccgtt cgtcaaccgc ctgcaaaccc accgtttacc
    21481 ggcaggaaat gccataaatc cccgcctatg ttaggataaa cgcctttacc ggcccacaca
    21541 aaggattatc atcatgaaaa aaatcatcat cgccgcactc gcagcggcag ccgtcggcac
    21601 tgcctccgcc gccacctaca aagtggacga atatcacgcc aacgcccgtt tcgccatcga
    21661 ccatttcaac accagcacca acgtcggcgg tttttacggt ctgaccggtt ccgttgagtt
    21721 cgaccaagca aaacgcgacg gtaaaatcga catcaccatc cccgttgcca acctgcaaag
    21781 cggttcgcaa cactttaccg accacctgaa atcagccgac atcttcgatg ccgcccaata
    21841 tccggacatc cgctttgttt ccaccaaatt caacttcaac ggcaaaaaac tggtttccgt
    21901 tgacggcaac ctgaccatgc acggcaaaac cgcccccgtc aaactcaaag ccgaaaaatt
    21961 caactgctac caaagcccga tggcgaaaac cgaagtttgc ggcggcgact tcagcaccag
    22021 catcgaccgc accaaatggg gcgtggacta cctcgttaac gttggtatga ccaaaagcgt
    22081 ccgcatcgac atccaaatcg aggcagccaa acaataaatc ttgatgcgaa tcagcacaaa
    22141 tgccgtctga acgtccttca aacggcattt gtgccgtctt catcaaaccg ccccgccaac
    22201 cgtaagccga tatggcggag tacaacccgc cgaaagatat ttcagcggca ggcagggctt
    22261 gccggaaagt ccggaaagta tagtggatta acaaaaacca gtacggcgtt ggctcgcctt
    22321 agctcaaaga gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt tccgtactat
    22381 ttgtactgtc tgcggcttgg tcgccttgtc ctgatttttg ttaatccact ataacacccg
    22441 aaaacaacgt ttcccgtttt cagacggcat acgatatgga caatttcatc cggccggtat
    22501 agtgttcgtc ctgctgcggc atctttccga tggcgcattt tttcaatatt aattatccga
    22561 cttacaaagg taaaaacatg actgtccgta tcgaacacga tttattgggc gaccgcgaga
    22621 ttcccgccga agtgtattgg ggcatccaca ctctgcgcgc cattgaaaac tttaaaatct
    22681 ccacacaaaa aatttccgac gtgccgcagt ttgtccgcag tatggtgatg gtgaaaaaag
    22741 caaccgcgca ggcaaacggc gaattgggtg cagtaaaacc cgaaatcgcc gccgccattg
    22801 aaaaggcttg cgacgaagtt ctgctgaaca accgctgcct cgaccaattc ccctccgacg
    22861 tgtatcaggg cggtgcggga acttcggtca atatgaacac caacgaagtc atcgccaacc
    22921 tcgcattgga agccttggga tacgaaaaag gccgctacga catcgtcaat ccgatggatc
    22981 atgtcaatgc cagccaatcg accaacgatg cctatcccac gggcttccgc cttgccgtgt
    23041 attacagcat cggcgaattg ctcgacaaac tgaccgtcct gaaaaacgcc tttaccgcca
    23101 aagccgaaga atttaaagac gttttgaaaa tgggtcgcac ccagcttcaa gatgccgtac
    23161 cgatgacggc aggacaagaa ttccaatctt tccaagtgtt gttggaagaa gaaatcctca
    23221 acctcgaccg cacgcgccaa ctgcttttag aagtcaattt gggcgcaacg gcaatcggca
    23281 cgggcgtgaa cacgcccaaa ggctacgccg aactggtggt caaaaaactc tccgaagtca
    23341 gcggcttgcc ttgcaaactg accgaaaacc tgattgaggc gacctccgac tgcggcgcat
    23401 atgtgatggt acacggcgca ttgaagcgca cggcggtcaa actctccaaa atctgcaacg
    23461 acttgcgcct gctctcttcc ggcccgcgtg ccggcttgaa agaaatcaac ctgcccgaat
    23521 tacaggccgg ttcttccatt atgcctgcca aagtcaatcc cgtgattccc gaagtggtca
    23581 accaagtctg cttcaaagtc atcggcaacg atacgacgat taccttcgcc gccgaagccg
    23641 ggcaactgca attaaacgtg atggagccgg tcatcgccca atgtatgttt gaaaccattt
    23701 ccctcttggg caatgcagca atcaacctgt ccgacaaatg cgtcaaaggc attacggtca
    23761 accgcgaaat atgcgagcgt tacgttttca actccatcgg gctggttact tatctgaatc
    23821 cgtatatcgg acaccacaac ggcgatttgg tcggcaaaat ctgcgcccaa accggcaaag
    23881 gcgtgcgcga ggttgtcttg gaacgcgggc tgttgagcga agaagaactc aaccgcatcc
    23941 tctctcctga aaacctgatg aacccccatc tgtaacgttg cagacaaatg ccgtctgaag
    24001 cagggatgct gcttcagacg gcattttgtt atatcaatca gccggattat atgccgaaca
    24061 gtaccttgac ttccccccaa taaaacaggc aggcgataga acacagtgcc aacacgattc
    24121 ccgccgtatt gattgtgttg attttttcct taaaaaacaa tgcgcccgaa agcgtaccca
    24181 aaacaatcac gccgatattc ataccggcaa aaaccaaggt cggattgtcc ttcatcattt
    24241 ggtgcgcggt gatgtaggtt acgatattca taaaattcaa accgcccaag agtatgccgc
    24301 cgaccacact ctctgcgcgc catctgaccg atttggcaaa caggcaggca aacatcagca
    24361 cacccgccag cacaaatgca accagcaggt tgcccgcaaa tgccgttccg cttttggcaa
    24421 gctgtttgaa caggatatcg ataatgccgt aacctgccca cacgcccagc agcaatgccg
    24481 cctgccgcca cgcgcttcct gattttttgc caccgctgtg tttccaaagc aggcagaaca
    24541 gtgcggcaaa ggcgaggcac agcccgatta gtttgccttc gctgagtttt tcgccaaaca
    24601 gcgttaaagc ggcaacaatc ggcaaaaaca gcgacaaacg ctgcgccgcg tcggatttga
    24661 cgataccggc ggattctacg gatttgccca ttatcacgaa tacggacggc agcagcacgc
    24721 ctaatgcggc aaacagcggc cacgtcggca aaaatgcgcc gatattgccg acatccggct
    24781 tcaataccag cagggtcagt atgaccgcga ccacataatt gacggcgacc gcctgcgcga
    24841 tgtcgatttt tttcttcctt gccattttca gcaaaatgga aaccgacacg ctgaacacga
    24901 tgctgattaa caggtaagcc atactatatc catttctcgg gtgcaaccgc cctgccgctg
    24961 ttgaagcgga taaagccgac gatgatgcgg tagaaatacc agacgttcgt tgcaataata
    25021 atcagcacgc cgatacccaa aagggcagtc agcgcgccca aaagataaag ccaaaatgtg
    25081 cgccaaaagg ttttaatcag gtattcggta tgtgtggcat agacaatgct gtccaaactg
    25141 ttccgtttca tataggcgag aatcacgccg actatgggca tataggcgaa aatcacgcca
    25201 actatgggca tgaacagcat gattgcaccc aaaccgaaca ggatgtaggc ggcgaggaca
    25261 tagttgcgcc cgcttccgcc gccggacggt tcgtaagcat ggcgcgaacg tgaggcctgc
    25321 cgttcaaacg cctgtctgaa cgcctgttcg cggcgcatct gttcgcggta aaactgctcc
    25381 ctgcgcgcct gttcgcggcg gaatgcctct tcctgccgcc cgcgttcctc atgccgtctg
    25441 aaggacgcgt cgtattgcat ccgtttcgac aggtcggaaa gcgtatcgta agcccgttgg
    25501 atttctttga acttttcttc cgcctgcgga ttgcccggat tgcggtcggg atggtatttc
    25561 atcgccagct tgcggtaggc gcgtttgatt tcgttcgcac ccgcctgcgg cgacacgccc
    25621 aatacggcat acaaatcttt gtccatattg ctccttatac ttcccggtca aaccgccgcc
    25681 tgtattgttt tcgggttcaa aatatttaaa tctccgggac ggatgccgat aatgaacccg
    25741 cgttttccgc cgttgatata gatgatgtcc aaatccatca ccgactgttc gacataaata
    25801 tccaattttg tccggatgcc gaacggcgtt gtgccgccga ccagatagcc cgtccacttg
    25861 tttgcctgtg ctggcgtggc gggttcgatg tgtttcgcac ccaaatgccg cgccagattg
    25921 cgggttgaaa tctgcttgtc gccgtgcatc aagacaatca gccccttacc gttttcatct
    25981 tgcaaaacaa tggttttaat gaccaagtgt tcgtctttgc cgaatagtcg ggcaaactgc
    26041 gccgtgccgc cgtgttcctc ataggcatag gtaaaaggtt caaattcgat gccgttttca
    26101 cgcaaaacgc gcacggcagg tgttatcgga taagtgtgtt tactcatttt tcgtcatgca
    26161 aaacatggaa atcggtcaag cgcaatgtta ccatatatcc cctttatcga aggaaaccga
    26221 tatggatatc cgatatttcg gcacaacgcc ccgctattcc gaagccgtcg gcgcaaacgg
    26281 cttgattttc ctctccggca tggttcccga aaacggtgaa acggctgccg aacagactgc
    26341 cgacgtgctt gcccaaatcg accgttggct ggcggaatgc ggctcggaca aggcacacgt
    26401 tttggatgcg gtcatctatc tgcgcgatat gggcgactat gcggaaatga acggcgtgtg
    26461 ggacgcttgg gttgccgccg gcagaacgcc cgcccgcgcc tgcgtggaag cgcggctcgc
    26521 ccgtccggag tggcgcgtcg aaatcaaagt aaccgccgtg aagaaagatg cggcaaccgc
    26581 ataaacagat ttcctttata tttcagagta cgcaaatgat gaaaacctta ctgccctttt
    26641 tcggcgtggt acgcgtcagc ggcgaagacc gccaaacctt cctgcacgga cagctttcca
    26701 acgacatcaa taatcttcag accgggcagg cgtgttatgc gacttacaac acgcccaaag
    26761 gccgcgtcat cgccaatatg attgtcgtca accgaggcgg cgatttgctg ctgattatgg
    26821 cgcaagactt gcttgaagca acggtcaaac gtttgcggat gtttgtgttg cgtgccaaag
    26881 ccgtttttga aatccttgaa gattacgccg tcggcgcgga attggaagca tccgacgaac
    26941 cgcttgccgc gcaagaaccc aaccttgcct ttgccgcaca acaagattca gacggcatct
    27001 gctctatcgc tctgcctcat ggcggcatcc ttcgcatcgc ccccaaaaac accttgccgc
    27061 cttatgatgc cgccgccgaa aacgcgtggc ggctgcacga aatccgtagc ggttatccgt
    27121 ggatatgcgc ggctaccaaa gaaactgccg ttgcccaaat gctcaaccag cacatcatcg
    27181 gcggcgtaca cttcaaaaaa ggctgctatc ccggtcagga aatcattgcc cgtgcccagt
    27241 atcgggggca ggtcaaacgc ggcttggcgg tattgtcggg aaattcggcc gtcgaagcag
    27301 gaaccctgct gacggcggac ggcgaagagg ccggcatcgt actcgacagc gttcaagatt
    27361 cggaaaactt taccgccctt accgtgatta aattctccgc cgcacaaaaa gaactgaccg
    27421 tcccaaacgg cggtatcttc aaagcggttc atctcttctt taaaaccgaa aatgcagaat
    27481 aagccgccga tgccgtctga agcccttcag acggcattgc cgccccgcca atcggttaca
    27541 atgccgcctt tccaattttc aaacccgaac cgttatgtcc aaaaaaacca agcaagaact
    27601 cgaaaacaac aaactcagca aacgcctgcg ccacgccgtc ggcgacgcga ttaacgattt
    27661 caacatgatc gagccgggcg acaaaatcat ggtctgcctc tccggcggaa aagacagcta
    27721 tgccctcttg gacatcctgc gccggctcca agccagcgcg ccgattgatt tccaactggt
    27781 tgccgtcaat ctcgaccaaa agcagccggg cttccccgaa gaagtattgc cgacctatct
    27841 cgaaagcatc ggcgttccct acaaaatcgt tgaagaagac acctactcca ccgtcaaacg
    27901 cgtattagac gaaggcaaaa cgacttgttc gctgtgcagc cgcctgcgcc gcggcatcct
    27961 ctaccgcact gcaaaagaat tgggctgcac caaaatcgcc ttgggacacc accgcgacga
    28021 catcctcgcc accttgtttt taaatatgtt ttacggcggc aaactcaaag ccatgccgcc
    28081 caagctggtg agcgacaacg gcgaacacat cgtcatccgt ccgctggcgt atgtaaaaga
    28141 aaaagactta atcaaatacg ccgaattaaa acaattcccc atcatcccgt gcaacctctg
    28201 cggctcgcaa cccaacctgc aacgccaagt catcggcgat atgctgcgcg attgggacaa
    28261 acgcttcccc ggccgcatcg aatcgatgtt ctccgccctg caaaacgtcg tcccttccca
    28321 ccttgccgat accgaactct tcgactttgt cggattggaa cgcggtcaaa gcctgaaaca
    28381 cggcggcgat ttggcgtttg acagcgaaaa aatgcccgaa cgtttttcag acggcagcga
    28441 agaagacgaa agcgaaatca aaatcgcgcc gcaaaaagcc gaacgcaaag tcatcaacat
    28501 tctggcgaac aagccgaaaa cctgcggcgc ataaacctcc gccgtcaaaa atgccgtctg
    28561 aagcccgttt ggcgtttcag acggcatttt aaaatcccga agtagacact tttcaacccc
    28621 aaacgcctgc tttttccgtt tcagctttct gaaggtttcc cgttatatta ctcggcaaca
    28681 acgcggcgga ttttgacggg ctgtacgggt acgttttgat aaaagccgcg cgtggcggtt
    28741 ttgacgcggg cgattttgga aacggtgttc atgccgcttt caaccctgcc gaaaacggta
    28801 tagccgtact gtccgttttt gtagttgagt gaatcattgt ccgccagatt gataaagaat
    28861 tggccggtgg cggaatccgg gtctgccgtc cgcgccatgg cgatggtgcc ggcggtgttt
    28921 ttcaaaccgt tgccggattc gttggcaacg gccttatcgc ttgccttttg taccaagtcc
    28981 tcggtaaatc cgccgccctg gataacgaaa ccgtcgataa cgcggtgaaa aacggtgtcg
    29041 tcgtaaaagc cttttcgggc atagcgcacg aaattagcaa cggttttggg ggctttggac
    29101 tcgtccaaaa ccaaacggat attgcccata tcggtttcca tcaaaacatg ggttgccgcc
    29161 atagacggca gggaaaccgc caaaagcagc gcggttaaaa cggttttgaa tttgggtttc
    29221 atcccgtcct cctcagacct tcagacggca ttttcatttc atatgccgtc tgaaggcgcg
    29281 tgaacgctat tccaatgcgt ctttgagctt ttgttcgatt aaatccgcat caaacgattt
    29341 ggcaatcaat tcaaaacgcg aatcgcgccg ccatgagact tggttcgcgc cccactgtcc
    29401 gtctacccag ttgagccaca cccacgttcc caatacttga aacacgcctt tggcgcggac
    29461 gagtccttcg gtcattttgg gcaggtcgtt gaaaaagttg gtcagctttt cgccgtcgaa
    29521 atcgcgtccg gcggggaatg tgaaaccttg cgactggaag cccatggtat tatccggcag
    29581 ggctttgagg cggtagcgtg atttttcgat gacgggaata tcgagccacc ggatgtcgag
    29641 ttgtgcgttt tggacttcaa ccactttggc tttgggcggg aacagttttg ccgctttgtc
    29701 gtgaaattcg gcaagctgtt cgggggtgca taaatcggtt ttgctggcga ccaatacgtc
    29761 gcagatgccg atttggtctt tatacaacgc ctgttgcgcg taatcggggt tgatgaactg
    29821 gcgcggattg acgacggtaa agacggcgcc gatttccaaa aggctgtcca gcggtttggc
    29881 tttgagttcg tcgatgacgc tggctgcgtg agccagtccg cttgcttcaa tcatcaggcg
    29941 gtcgggcttg gcgtcgcgca gcattttctg cacggttacg cccatttgcg ggccggaggt
    30001 gcaacacaaa cagccgccgg cgatttctgc cacagggata ccgttgtcgc tcaataccgc
    30061 gccgtcaatg ccgatttcgc cgaactcgtt gacgatgatg acccattttt cgttcgggtc
    30121 tttttgcgcc atcaggcttt tgagcgcagt ggttttgcct gttcccagaa aacctgaaat
    30181 caggtggact ttggtttttt tcatttctga cattttttac agattccagt taaaacaacg
    30241 tgttcttctt tcagcgcaaa gccgctttcg gcaacgcctg cgcgcagtgc cgcccactcg
    30301 tggctgaggg tttgctcgtc cgccgtgccg cattcggtgc agaccaaaat aaacgcgctg
    30361 tggtgcgctt cggcttcttc gtggtcgtgg caatggtcgt cgcactcgtg ctgcgcgtgg
    30421 ctgcacaaaa tatagccgtt gaccgccgcc actttgtgca aaacgccctg ctccgcccaa
    30481 aaatcaaggg cgcggtaggc ggtaggcggt gcaagcacgc cctcgctttg ctgctgcatc
    30541 tgcgacaaga cgttgtaggc tttaatcacg ccgctttgct gcaagacaat atctaatacc
    30601 tgctcgcgca aagcggttac ctgcaagcct tcgctgcgtg cctgttcgat aattttctgt
    30661 ttgaaatttg ttttcataaa ttctctgttt atgccgtctg aacaaccgat acggcaggag
    30721 gcggttttat atttgtattc aattgcttta tttggaaatc ttttccaaca atgcccgaca
    30781 gcccgcatcc gcaagcctga aggtttcttc aaaatcgccc gtgtaccacg gatcggggac
    30841 atggtcgtaa ccgctttcgg gtatcaggtc ggtcagcttg aatatttttt ccggccgcct
    30901 gccgaaggtt ctttccaatt cggacaaatt cttgccgtcc atcgcgatga tgcagtcaaa
    30961 cgccgccgca tcgctttggc ggattttgcg gctggtaaag cctgaagcat cgataccgtg
    31021 ttttttcaat atctttgccg tctcgcggtg catatcttcc ccgtcgtgcc agcccgatgt
    31081 ccccgcgctg tccgcttcaa ggggaatgcc cgcttcggcg gcgcggcggc gcaaaatgta
    31141 ttccgccatc ggcgaacggc agatgttgcc gaggcagaca aaaaggattt tcggtttttt
    31201 catatcccct ccctgttccg gcgcgatgcc gtctgaagcg gaaacccttt cagacgtcat
    31261 cggtcggtta atcgtaaccg cataaggcag aaccagccaa aacatccatc tggaagaaat
    31321 ggtttggcta aatcttaggc atatttaata agtgtccaat attagaagcc gtatgctcca
    31381 aatagagact ggcatttttc aaactatctt ctaaaggttc acttttctcc aaaatagaaa
    31441 aggcagcttg gatattttca aatggcaggg aaggcaaatc ttcaacgaga ctgccacaaa
    31501 tagcgacaac aggaactccg acaggggttc tttttgctac accaataggc gctttccctg
    31561 ctaaactttg acgatctagt cttccttcac caatgataac caagtcaaca tctgacactt
    31621 tcttatcaaa gtcaatcaag tccaggcagg tgtcaattcc agatacgata cttgcctgag
    31681 caaaggcgca caaaccacca gcgatgcctc caccagctcc tgctccttta agttttaatg
    31741 ttgcagggga gactttttca taaaaatctt gtattgcctg atctacggcc tcaaacatag
    31801 tagaatccaa ccctttttgc ttgccaaacg tataggtcgc accttggtgt ccacataagg
    31861 gactcacaac atctgctaaa atacgaatgt gaacatcttc aggaatttca tagcgatttt
    31921 ctgttgaaac agaagctagg tttagtaagg cttgaccgca agtgggcaag gtatttccat
    31981 cctcatcata aaattgataa cctaaaccag cagcaatccc aatacctcca tcattactgg
    32041 ccgtaccgcc aacgccaata tagatttctt taatttcttg actaatgagg tggcgaatca
    32101 attctccaat accacgagtt tggatttgta atggatttcg tttctctagc gggatttttc
    32161 caagaccaac caaatcagca acttcgaata gggctagttg ttctttttga aaatagcgca
    32221 tgacttcttt ttgtccaaaa ggtcctgtca cttggagaca tttttcttct aggtcaagag
    32281 aatgtcggat agcatctaca gtgccttctc ccccatcacc gacaggacag aggagacatt
    32341 ccacatctgc tatcgcttgt tggaagcctc tttttattgc ttcagctacc tgttgagctg
    32401 tcaagctttc cttaaacgaa tccggtgcaa ttacaatctt catatttata attcatcctt
    32461 tcgtttcact caaggcacaa cacagaatga aaaagtgttg tgctctttat tttgatttat
    32521 tatagaaatg agaaagccta tcactactac aaatcactat gcgctgaaaa acggattgtg
    32581 cccttcccgt ttcaatgctt ccgcatagct cgggatgctt tcctgttcgc ccaagggatt
    32641 gtgcaacagg taaacgtgtt ctacacggca ttctgccatt aaatccaaaa aattttgctg
    32701 aacaacggcg atattgtcgg taaaggaaag ctgccacgaa aacacctgcg gcacgacttc
    32761 aaacgcgccg tcggtcgcat taaatccgaa tagaatctgt ccacctacct ttgccgcagc
    32821 gaccacgccg acgcgcgggc tctgcatacg gacggcgcgt atcgcgtttg ccgcaaaaac
    32881 atccatcgcc atacgttggc gcgtgtggct gccctctgaa agcgcgcgta ccggagaatc
    32941 cggggaaagc gggttggcaa acagcgtaac gccttccgat gcaagatttt cctgccatac
    33001 ctgcatcaag ggcagacctg cctgaagaag gttctgaccc aatgccgtct gaactttgcc
    33061 gctaccgaaa cccagcgcgt aaacgacgtg gacggactgc ccttcgggca acagcaaagg
    33121 gcgcggggcg aaacggcgta agtgttgcgc tgccgcttcg gtattttgtt tggcacgcca
    33181 ccactcatcc ggcgcgaccg cacttaaatc ggaagaatgc acaagataag gcagccattg
    33241 tgtctcttgc gccaacgcgc gcaggtgcgg atagttttgc aaacaggcaa acaatgcctc
    33301 cgtcggcagc ttcatctcga ttgcccgttc tttcttgcag ccggacacca gcacgaccgg
    33361 cagggcgaac cactgcactt cgccttcttt ctcgcaatcg agtaccgcgt tcacactgga
    33421 aagcagcgcg gcataagttc cggcatcggg cgacatcgtc agcgcgaggg aaaggttgat
    33481 atagtggttt tgttcaagca tccccctgat ttcggtttga agctgcccgg acgagagttt
    33541 gcgcgaagcc tgggaagaat tgtgcgccaa ctggtaggca ttgagcagca ggtggttctt
    33601 aatcggattt tggggatacg ggcgcgtatc gggcaaggta aatgtctggt tcatatatgt
    33661 cgtgtaccgc ttttcgcagt gtgtgttttt cctgtttgaa aatatatcgg acggattgcc
    33721 gtcgggactt gcccgtcaat ccgccgaaac gagaaaatgc ctgtctgcca agtctgccaa
    33781 tatttcttcc acatacactt cggcaggcgg atggaatgtc aaaccgtcgg gcgtggtgct
    33841 gacgatgttc aagttttcag caaccgcatc tatcaccgcc tgtttgctcg tcggccgcga
    33901 caattgccgc ataatgtaca aatgcccgta tccgaatgag ggggtgcttt cgttgtaacg
    33961 gttggcaggc cggacaaacg cttccgcgcc gtgttccaca aaggcggcgg cataatctgt
    34021 aaaacgcgcc ggcacatagg ttttatggtc ttcaaaaaat ggtttgcccg cattgtctga
    34081 tgaaacagaa aaacgcccga gaatggtttg caataaaagc tgggaattga ttttcatacg
    34141 gtcaaattcc agaccgggaa atcggcgggc gaggttttcc gcaacatctt ccgttttaaa
    34201 cgcctctccg gttttcatca catcgcgtat gcccgaaagc agggtatcat tttcatcaaa
    34261 gttgattgtt tcccctcttg ccgggcggaa attcaaactt tctatcactt cgacggcaac
    34321 cgactcatcg cgcctgatat tgtccccgac ttcctcacgg cataaaagcg aacggcggaa
    34381 ttggcggtcg gataaaatat cgctataaaa ttctttggca atataatcgc cccctgccaa
    34441 tgccagaatc cgctcccgcg tatgctccgc catccaagaa acaaaagacg cgtgcaaatc
    34501 ggtatccccg atatatgcga gcctgtggcg attagcccat tcgatgaagc cgttgacgta
    34561 aatcgggtcg ttaaacgcct ccatatattc gtgtgcgatg taataaaaat tatgattcaa
    34621 tattttttga atcgccggaa gtttgccgcc gccgtccaag cccttgtcgt tttccaaaat
    34681 ttccgccagc gccttgagcg cgtccaagcc tttccgcgtc cgcgcttcca agggttcttc
    34741 aagcacatcc ctgccggcaa agtacataat ttcgcgcaac tgctcctgcc gtttccagcc
    34801 cgggtaaaca ttgtatgaaa tataggcaat gccgtgttcg gtcaggttgt tccggcaaat
    34861 cgaaaaaatt ttgtctttaa ctgcgtcagg cacccacgac caaatgccgt ggacgatgat
    34921 atagtcaaac ttcccgaatg actcatcgat ggtcaaaata tctttttctt ccagacgcac
    34981 atttttcaag cccatttttt caatgatggc gttgccctgc gcaacctgcc tgccggacaa
    35041 gtcgataccg acaaattccg catccgggta ataaagagcc tgcgtgatga tattgccgcc
    35101 catcgaacag cccagctcca agaccttggc ttttgccgct gctgcaggct tcaggcccat
    35161 catgcgggcg cgagcctcca aggcgttgat ggcggtttgg gaaaatgctt tggattcata
    35221 catcaaatca tcatatgaat ttttgatgtt ggacacgtcc ggcacaccgt tctccgttgc
    35281 agcacgcgca aaggcggctt ttttcttttt gttcggcata ttttgtttgt ctgaaggcac
    35341 tattgcccgc aagtttaacc aattcatcct acccgttcaa ctaaatcaaa tgccgtctga
    35401 aggcgcggag cgtacttcag acggcatcgg ggaggcgcga aggcttcaga cggcattttt
    35461 gccgctttat ttcaacgcgg catcatagcc gccgtcttca acggcttcaa tcaacgcccc
    35521 tgcatcggtt tgcgcggggt cgtatccgac ggtcgcactt ttgttttcaa gactgacttc
    35581 gacgcttgcc acgcctttta cgccttccaa tatccgggta acgcttttga cgcagccgcc
    35641 gcagctcatg ccgccgatgt cgaggataag ggtttccatc attttccttt cgttggaact
    35701 gcaatctgac gggcgttatt gtaagtcggg gcgtgaactt gggcaaacgc ggaaacggtg
    35761 cggcggtttg gaaaaagacg gacgcttgcg cataatggcg gcaattccca tcaggacgac
    35821 aacaatgaac gcttcgcaaa aaccctggtt gagcatcatc gccttggcaa tcggcgcatt
    35881 tatagtggat taacaaaaac cagtacg
//
LOCUS       DAWFIE010000012        34536 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWFIE010000012 DAWFIE010000000
VERSION     DAWFIE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34536
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.012"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            80..1045
                     /locus_tag="WGQ71_000549"
     CDS             80..1045
                     /locus_tag="WGQ71_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0706612.1"
                     /translation="MLRLTALAVCTALALGACSPQNSDSAPQAKEQAVSAAQTEGASL
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSIDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKKAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGLPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            1063..1212
                     /locus_tag="WGQ71_000550"
     CDS             1063..1212
                     /locus_tag="WGQ71_000550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706613.1"
                     /translation="MPSETRMQTASCVSDGIARYGGFKQGFPLRRFGLPCLNLLRLNA
                     FPFCL"
     gene            1209..2177
                     /locus_tag="WGQ71_000551"
     CDS             1209..2177
                     /locus_tag="WGQ71_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0706614.1"
                     /translation="MTAKPFSLNLTNLLLLAALFVVSLSVGVADFRWSDVFSLSDSTE
                     LMLVSRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMMLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSGVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFVGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            2167..3141
                     /locus_tag="WGQ71_000552"
     CDS             2167..3141
                     /locus_tag="WGQ71_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ0706615.1"
                     /translation="MPSEKNIGFMAGSSRPLWAAFALLLICCGLFLTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAAVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCIGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLRHKK"
     gene            3186..3812
                     /locus_tag="WGQ71_000553"
     CDS             3186..3812
                     /locus_tag="WGQ71_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706616.1"
                     /translation="MTQERFPSFFNQAPTITVRDALAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALKPFAPE
                     MRELMPKAVGGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            3896..4654
                     /locus_tag="WGQ71_000554"
     CDS             3896..4654
                     /locus_tag="WGQ71_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0706617.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLADTPTAELAKTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADHVVAMKNGKAVLTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(4713..5051)
                     /locus_tag="WGQ71_000555"
     CDS             complement(4713..5051)
                     /locus_tag="WGQ71_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ0706618.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            5146..5358
                     /locus_tag="WGQ71_000556"
     CDS             5146..5358
                     /locus_tag="WGQ71_000556"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706619.1"
                     /translation="MPETVIPAQAGIPISAFQQYLKVSVNPNFHIPNRAETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            5515..9477
                     /gene="purL"
                     /locus_tag="WGQ71_000557"
     CDS             5515..9477
                     /gene="purL"
                     /locus_tag="WGQ71_000557"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ0706620.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKTLDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     DAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPGLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPIDLPLNVLLGKPPKTTRSDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNDRSALFADVKFDVNEDIAAPFVNSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRVNLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPNTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGKISDDLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEDWTELSGWYRLFAGARKALG
                     "
     gene            9572..10330
                     /gene="gloB"
                     /locus_tag="WGQ71_000558"
     CDS             9572..10330
                     /gene="gloB"
                     /locus_tag="WGQ71_000558"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ0706621.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            10653..15146
                     /gene="mspA"
                     /locus_tag="WGQ71_000559"
                     /gene_synonym="ausI"
     CDS             10653..15146
                     /gene="mspA"
                     /locus_tag="WGQ71_000559"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HEZ0706622.1"
                     /translation="MRFTHTTPFCSVLSTLGLFAVSPAYSSIVRNDVDYQYFRDFAEN
                     KGAFAVGASNISIQDKQGKILGRVLNGIPMPDFRVSNRQTAIATLIHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPTALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTRTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAIAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDNFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGDSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPVLSPEHVEWVKWGNRPQGNAAVYEYINTHRNRRTDYFILK
                     PGGNPREFFPLNMENSTSWQFVGNNRQQAAEQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNIEWVNSPNTPHKPSPYTNTPTRTGAGVPTITASKKAATPAPTSHGMPTA
                     PKTGNFSDITGEAAIEKTRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMN
                     LNGEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAG
                     RNTAHLDGDITAYDLSGIDLGFIQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPAT
                     QVRGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQ
                     ITLNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPPLKLEGDSRGAFQI
                     HVKNTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNP
                     LKEAELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARI
                     DSQVQYLSAQLKQTDPLTGILTRAQNLCAAQGYSVDICRQVAKAADTNDLTLFETELD
                     TYIERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNL
                     AELISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTHR
                     PYQQTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENG
                     ALFAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGIKIDTGINLRPYAGI
                     RINRSNGNRYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQN
                     SGSALSVNDRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIG
                     YNW"
     gene            15378..16832
                     /gene="mgtE"
                     /locus_tag="WGQ71_000560"
     CDS             15378..16832
                     /gene="mgtE"
                     /locus_tag="WGQ71_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ0706623.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNVVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITVDEMVDVIREESEADMLNMAGLQEEEDLFAPVWDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(16918..17826)
                     /gene="hslO"
                     /locus_tag="WGQ71_000561"
     CDS             complement(16918..17826)
                     /gene="hslO"
                     /locus_tag="WGQ71_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ0706624.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGRGRLKMLVAEAASDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGGGIAQMLANYMKRSEQIDT
                     HIVLSASDEAAGGLLVQRLPEAVLDEEAWEHVSTLARTLTADELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            18071..18826
                     /locus_tag="WGQ71_000562"
     CDS             18071..18826
                     /locus_tag="WGQ71_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0706625.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            18845..19063
                     /locus_tag="WGQ71_000563"
     CDS             18845..19063
                     /locus_tag="WGQ71_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706626.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            19208..19552
                     /locus_tag="WGQ71_000564"
     CDS             19208..19552
                     /locus_tag="WGQ71_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ0706627.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            19552..20244
                     /locus_tag="WGQ71_000565"
     CDS             19552..20244
                     /locus_tag="WGQ71_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ0706628.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            20311..21531
                     /gene="argJ"
                     /locus_tag="WGQ71_000566"
     CDS             20311..21531
                     /gene="argJ"
                     /locus_tag="WGQ71_000566"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ0706629.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLSFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVVD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQSAATVYTCD
                     LSHGYVSINADYRS"
     gene            21591..23000
                     /locus_tag="WGQ71_000567"
     CDS             21591..23000
                     /locus_tag="WGQ71_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0706630.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETTAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            23190..24581
                     /gene="hrpA"
                     /locus_tag="WGQ71_000568"
     CDS             23190..24581
                     /gene="hrpA"
                     /locus_tag="WGQ71_000568"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ0706631.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYRPLIGKDEDDAEIELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIQLFSEENFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            24682..25809
                     /locus_tag="WGQ71_000569"
     CDS             24682..25809
                     /locus_tag="WGQ71_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706632.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            25989..27380
                     /locus_tag="WGQ71_000570"
     CDS             25989..27380
                     /locus_tag="WGQ71_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ0706633.1"
                     /translation="MKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALTF
                     VIAALYLFARYKTSRLLIALFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGSA
                     GASMLDKLWLPALWGVAEVVLFCSLAKFRHKTHFSADILFAFLMLMIFVRSFDTKQEH
                     GISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSRIGQGSIQNIVLI
                     MGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIPH
                     ANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLGY
                     GNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDKY
                     DNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPLV
                     LYSPNKAVQQAAN"
     gene            27629..31093
                     /locus_tag="WGQ71_000571"
     CDS             27629..31093
                     /locus_tag="WGQ71_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HEZ0706634.1"
                     /translation="MQNMKNQIRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLAYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQT
                     AIEMGLTTKEAAFRRLSEIKQLTSSENQGDQDLSAKRKQKQLDKKQHRAQIRAAKEAG
                     YEQIHRALLTGLIANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TKLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRP
                     VSYGKVAPEEAREIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYNERLPEMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSR
                     QNENHGNTVGWVENPTSAATAKTVGFDNPTYATQQPTPSPEREGRGEGKTVAAQTNFS
                     ATAANPLPQEREQSASASTFSDDLRPANLQQTAPSPVGEGWGEGKTVATQTNFSATST
                     NPLPQEREQSASASTFSDDLRPANLQQPSPSPVGEGWGEGKTVATQTNFSATTANPLP
                     NPLPQEGEQSAAASAVSNDPQPKKQPAPQKNRLKPLPLADIRTFQAWLKTAERDNPRL
                     LFLSRDDLMQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTMTVPLTVLNR
                     LHAPSLEWLVPGMIREKIQLQIKALPKQIRRICVPVPEFITQFLSQNPDRNAPILPQL
                     AQAIAKTAGDIRIFEQINQDEWAAFRLPEHCYFNLRIIDDGGQELAGGRKLHELQQQL
                     GQAAAVTFRDNTQEFERDNVTAWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALR
                     LFDTTEAAEQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLT
                     QAVCDRAFIGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAVYAELNSKLG
                     KHPLTHLLRLRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREA
                     DIQELEQMWQEKTDSLIKQGLPISDGLAAFKWMIEELRVSLFAQELKTPYPVSVKRLL
                     KEWEKIEK"
     gene            complement(31126..32217)
                     /locus_tag="WGQ71_000572"
     CDS             complement(31126..32217)
                     /locus_tag="WGQ71_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEZ0706635.1"
                     /translation="MQTFNRERLEFALDKRGCTQTKLAELLGITARQVRNYVKDEQIP
                     DLNELANLLEFPVHFFTNDERLPELKSQAVSFRARSRTSKRLEKQALNHSITAFLLND
                     WLETEFTLSQAVLPDFSDISPEEAAQELRLEWGLGNEPIPNMITLLEAKGVRVFSLSL
                     DTKDIDAFCTWYENHPFVFLNTQKSAERSRFDAAHELGHLIRDKYSMEHSKNSEITED
                     EPRDIIEKEANAFASAFLMPEAALRLYRHVPITIENLLKIKRRFGVSLVALAYRMHKL
                     GMITDRMYIHNLCPLFAKKKYRTIEPEPMEREISSALRKMLTILKNDGIGIEQIAENL
                     NVSPKDVADLTFGLVERDLNRYRKLRLVK"
     gene            complement(32233..32877)
                     /locus_tag="WGQ71_000573"
     CDS             complement(32233..32877)
                     /locus_tag="WGQ71_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706636.1"
                     /translation="MVEFALSEPFPFEVGEIVEQQNSDIALSEISKYLSQEMLKEIVL
                     TAHNNIQDKAALSDRNTLGMYRQAEITFALRVYLSKDGWTLEPWSGGAFLCLSPCRTK
                     SIIVATATSDVGRHHGMPMTTARKGKGFEEAIRNSKLDLPDTVQFWVLLFHWKYNKVQ
                     LELSLPSGFVSHQITGYKQRIILADVDLTKFDELERKQPVEEYEPLVERKQKAG"
     gene            33234..33446
                     /locus_tag="WGQ71_000574"
     CDS             33234..33446
                     /locus_tag="WGQ71_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706637.1"
                     /translation="MSGIYLPRLFPPHIAERGLLYFQQGKVLDVRKTSAGHYRAEVCG
                     SENYWVWLKLDSDLYIKDESCDCPYV"
     gene            33743..33898
                     /locus_tag="WGQ71_000575"
     CDS             33743..33898
                     /locus_tag="WGQ71_000575"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ0706638.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(33988..34437)
                     /locus_tag="WGQ71_000576"
     CDS             complement(33988..34437)
                     /locus_tag="WGQ71_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0706639.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         8698 a        10317 c         8221 g         7300 t
ORIGIN      
        1 tatcatttat cctttctaaa gccgttccgg tttgtccgac cggcggcttt gccccaatat
       61 ccccattttg gagacaccta tgttacgttt gactgcttta gccgtatgca ccgccctcgc
      121 tttgggcgcg tgttcgccgc aaaattccga ctctgcccca caagccaaag aacaggcggt
      181 ttccgccgca caaaccgaag gcgcgtccct gaccgtcaaa acggcgcgcg gcgatgttca
      241 aataccgcaa aaccccgaac gtatcgccgt ttacgatttg ggtatgctcg acaccttgag
      301 caaactgggc gtgaaaaccg gtttgtccat cgataaaaac cgcctgccgt atttagagga
      361 atatttcaaa acgaccaaac ctgccggaac tttgttcgag ccggattacg aaacactcaa
      421 tgcctacaag ccgcagctca tcatcatcgg cagccgcgca gccaaagcgt ttgacaaatt
      481 gaacgaaatc gcgccgacca tcgaaatgac cgccgatacc gccaacctca aagaaagtgc
      541 caaagagcgt atcgacgcgc tggcgcaaat cttcggcaaa aaggcggaag ccgacaagct
      601 gaaggcggaa atcgacgcgt cttttgaagc cgcgaaaact gccgcgcaag gcaaaggcaa
      661 aggtttggtg attttggtca acggcggcaa aatgtccgcc ttcggtccgt cttcacgctt
      721 gggcggctgg ctgcacaaag acatcggttt gcccgctgtt gacgaagcca tcaaagaagg
      781 cagccacggt cagcctatca gctttgaata cctgaaagag aaaaatcccg actggctgtt
      841 tgtcctcgac cgaagcgcgg ccatcggcga agagggtcag gcggcgaaag acgtgttgaa
      901 taatccgctg gttgccgaaa caaccgcttg gaaaaaagga caggtcgtgt acctcgttcc
      961 tgaaacttat ttggcagccg gtggcgcgca agagctgctg aatgcaagca aacaggttgc
     1021 cgacgctttt aacgcggcaa aataatgaaa cggcggcatt cgatgccgtc tgaaacacgg
     1081 atgcaaaccg cctcctgtgt ttcagacggc attgcccgat acggaggctt caaacaaggc
     1141 tttccgctcc gacggttcgg actgccttgt ttgaatcttc tacgccttaa cgcttttccc
     1201 ttctgtttat gactgccaaa cctttttccc tcaacctgac caacctgctg ctgctggcgg
     1261 cgttgtttgt cgtcagcctg tcggtaggcg ttgccgattt ccgctggtcg gatgtgtttt
     1321 cgctgtcgga cagcaccgaa ctgatgctgg tcagccgcct gccgcgcacg tttgcgattg
     1381 tgctgacggg cgcgtcgatg gcggtggcgg ggatgattat gcagattttg atgcgtaacc
     1441 gttttgtcga gccttctatg gcgggcgcgg gtcagagtgc ggctttgggt ttgcttctga
     1501 tgtccctgct gctgcctgcc gcgccgctgc cggtcaaaat gtcggttgcc gccgttgccg
     1561 cgctaatcgg gatgttggtg tttatgatgc ttatccgccg cctgccgccg accgcgcaac
     1621 tgatggtgcc gctggtgggg attattttcg gcggcgtggt tgaggcggtg gcgaccttta
     1681 tcgcgtatga aaacgaaatg ctgcaaatgc tcggcgtgtg gcagcagggc gatttttcag
     1741 gcgtactgct ggggcggtac gagctgcttt ggattacggg cggtttggcg gtgtttgcct
     1801 atctgattgc cgaccggctg acgattttgg ggctgggcga gacggtcagc gtgaatttgg
     1861 gtttgaaccg gacggcggtg ttgtggtcgg ggctgattat tgtggctttg attacgtcgc
     1921 tggttatcgt tacggtcggc aatattccgt ttgtcgggct ggtcgtgccg aacatcatca
     1981 gccgcctgat aggcgacagg ctgcgccaaa gcctgcctgc ggtcgccctc ttgggcgcgt
     2041 ctttggtttt attgtgcgac attatcggac gcgtgattgt gtttccgttt gaaattccgg
     2101 tctctacggt ttttggtgtg ttgggtacgg ctttgttttt gtggcttttg ttaaggaaac
     2161 ctgctcatgc cgtctgaaaa aaatatcggt tttatggcag gaagcagccg tccgttgtgg
     2221 gcggcattcg cacttttact gatctgctgc ggactgtttt taacgctcaa cgtcaaaggc
     2281 gattgggact ttgttttaca cctgcgcctg accaagcttg ccgcgctgct gatggtcgcc
     2341 tatgcggtcg gcgtgtccac gcaactcttc caaacgctga ccaacaatcc gattctgacc
     2401 ccttcgattt tgggtttcga ttcgctgtat gtgtttttgc agaccttgct ggtgtttacg
     2461 ttcggcggtg tgggctatgc ttccctgccg ttgacgggca aattcggctt tgaactggtc
     2521 gttatgatgg gcggctcgct gctgctgttt tacacgctca tccgtcaggg cggacgcgat
     2581 ttgccgcgta tgattttaat cggcgtgatt ttcgggattt tgttccgcag cctgtcgtcg
     2641 ctgctttcgc gcatgataga ccccgaagaa tttacggcgg cgcaggcgaa tatgtttgcc
     2701 ggattcaata ccgtccacag cgagcttttg ggcataggcg cgctgattct gctcgtcagc
     2761 gcggcggcgg tttggcgcga acgctaccgc ttggacgtac accttttggg gcgcgaccaa
     2821 gccgtcaatt tgggcatcag ctacacgcgc aacaccttat ggatactgct ttggattgcc
     2881 gcattggtgg cgacggcgac cgccgttgtc ggcccggtaa gctttttcgg gcttctcgcc
     2941 gcctcgcttg ccaaccactt ttccccgtcc gtgcgccatt ccgtccgcct gccgatgacg
     3001 gtttgtatcg gcggtatcct cttggtcggc ggacagaccg tattcgaaca cttcttgggc
     3061 atgaaggcgg tattaagcgt ggtggtcgaa tttgcgggcg gactcgtttt cctctatctc
     3121 gttttaagac acaaaaaatg acggatgccg tctgaacggc agcccgactc gaaaggacaa
     3181 accatatgac gcaagaacgt ttcccatcat tcttcaacca agccccgacc atcaccgtca
     3241 gggacgcgct tgccgcattc ctcggcgcgg ccgaaaatgg catcctcact taccgctacg
     3301 ccgatgccgt gcgcctgtgc ggccactcct gcccgaccgt cgccggcgcg tacctgatgg
     3361 ttatcaaagg tctgaaagcc ctttacggcg aagagctgcc cgaacgcggc ggcatcgaag
     3421 ccgccatgca gggagcgcgc gacgaaggca cggtcggcgt aaccgcgtcc gtcgtccaac
     3481 ttctcaccgg cgcagccccc gaaaccggct tcggaggcat cggaatgcag ggacgcttcg
     3541 cccgccgcaa cctcttatcc tttggtgcag gcgaaatcaa cggcacactg accctgcgcc
     3601 gcaaagacaa cggcaaaacc gtcgccgtca gcctcaacgc cgccctgaaa cccttcgcac
     3661 ccgaaatgcg cgaacttatg cccaaagccg tcggcggcag cgcaagcgca gaagaactcg
     3721 aacgcttcgg acaactctgg caggcacgcg ttaaagcatt tttgaccgaa tcggcggacg
     3781 acccgcagtt cgtcatcgtc cgcgaagtgt gaacgttcag acggcattcc gaatttcaaa
     3841 tgccgtctga acccccgcta aacaacaaca aacctacgcc cgacaagcat ccgccatgat
     3901 taccatccgc aacgtcagct accgcatcgg cacacgcccc atcctcgaca acgtcagcct
     3961 cgacatcccc gaaggcggca ttaccgccct cgtcggcccc aacggcgcgg gcaaatccac
     4021 cctgttttcc tttatggcgc ggctgcgccc gcttgaaagc ggcagcatcg cctaccgcgg
     4081 caaaaacctt gccgacaccc ccaccgccga actcgccaaa actctgtcca tcctcaccca
     4141 agaaaacagc atcatgagcc gcattaccgt gcgcgacctg ctgatgttcg gacgttaccc
     4201 ctaccatcaa ggcagaccga ctgccgaatg ccgccgtatc gttaacggtg caatcgaaga
     4261 attccacctg caagacctct ccgaccgcta tctgaccgag ctctccggcg gccaacgcca
     4321 acgcgccatg attgcgatgg tgttctgcca aagcaccgac tacgtccttt tggacgaacc
     4381 gctgaacaac ctcgatatgt atcacgcccg ctcgctcatg cagattctgc aaaagctcac
     4441 gcgcgaacac aaacgcacca ccgtcgccgt attgcacgac atcaaccagg ccgccgccta
     4501 cgccgaccac gtcgtcgcca tgaaaaacgg caaagccgtc ctgaccggca cgcccgaaga
     4561 agtgttcacc gtacacaaca tcaaagaact gttcgacatg gacgtggacg tactcgacta
     4621 cgaaggcaaa aaactcatcg tccaccatat ctgaagctca aaaagaaaat gccgtctgaa
     4681 aggctttcag acggcatttc cattccaaac gctcaaaccg ccgcgtccgc acgttcgccc
     4741 gtgcggatac ggattgcctc ctcgaccggc agcacaaaaa tcttgccgtc gccgattttg
     4801 cccgaacgcg ccacctcgac aatcacgtca atcgcgcgtt ccacagcgtc atccgccaac
     4861 accagctcga ttttgacctt gggcaggaaa tcgacggcgt attccgcacc gcgatagatt
     4921 tccgtatgcc ccttctgcct gccgaaccct ttgacctcgc tgacggtcat ccccgtaatg
     4981 ccgatttccg tcaacgcctc gcgcacgtcg tcgagtttga acggtttgac aatcgcctcg
     5041 atttttttca tcacattctc cattaattaa cgtaaacaaa cgacaaatat agcagaaacc
     5101 gccggcaaac gataaacggt actgttcgcc cggcgatgcg gcgatttgcc ggaaaccgtc
     5161 attcccgcgc aggcgggaat cccgatctct gcatttcagc aatatttaaa ggtttctgta
     5221 aatccaaact tccatattcc taaccgtgcg gaaacgacgg catttgatct aaaaacatac
     5281 ggaagcagtt gcccgcccct ctacttcctg caattatccg catacccggc tttgggtaaa
     5341 aatcagaaaa acagataaaa cccttattcc ttaatatctt cagacggcac ctgtattttc
     5401 cccaactgta tgccgtctga agattgtcaa cattttaagc caaaatatcc aagcaataca
     5461 aacctgtccg gcgtataatc cctctttttc ccaaccgcat ttgtgagccg aattatgtct
     5521 gtcgttttgc ccttgcgcgg cgttaccgcc ctttccgatt tccgtgtcga aaaactcttg
     5581 caaaaagccg ccgcactcgg tctgcccgaa gtcaaattaa gcagcgaatt ttggtatttc
     5641 gtcggcagcg agaaaacact ggatgccgca actgtcgaaa aactgcaagc cttgttggcg
     5701 gcgcaaagcg ttgaacaaac gccaaaagcg cgcgagggct tgcatttgtt tttggtcacg
     5761 ccccgtttgg gtacgatttc gccgtgggct tccaaggcga ccaatatcgc ggaaaactgc
     5821 ggtttggcag gcatcgagcg catcgagcgc ggcatggcgg tgtggctgga aggtgcgctt
     5881 accgatgaac agaaacagca atgggcggct ttgctgcacg accgcatgac cgaatccgta
     5941 ttgcccgatt ttcagacggc atccaaatta ttccaccatc tcgaatccga aaccttttcc
     6001 accgttgatg ttttaggcgg cggtaaagag gctttggtca aagccaatac cgaaatgggt
     6061 ttggcactct ccgccgacga aatcgattat ctggttgaaa actatcaggc tttgcagcgc
     6121 aatccgtccg atgttgaatt gatgatgttc gcgcaggcaa acagcgaaca ctgccgccac
     6181 aaaatcttca acgccgattt catcctcaac ggcgaaaaac aaccgaaatc cctgttcggc
     6241 atgatacgcg acacgcacaa cgcgcatccc gaaggtacgg tcgtcgctta taaagacaat
     6301 tcgtccgtaa tcgaaggcgc gaaaatcgag cgtttctatc cggatgcggc ggaaaaccaa
     6361 ggctaccgtt tccacgagga agacacgcat atcatcatga aagtggaaac gcacaaccac
     6421 ccgaccgcca tcgcgccgtt tgcgggcgcg gcgacgggcg cgggcggcga aatccgcgac
     6481 gaaggtgcga cgggcaaagg ttcgcgtccg aaagcgggct tgaccggctt taccgtgtcc
     6541 aacctcaaca tccccggcct caaacagccg tgggaacaag attacggcaa gccggaacat
     6601 atttcctcgc cgctggacat tatgattgaa ggccccatcg gcggcgcggc gttcaacaac
     6661 gaattcggcc gccccaacct cttgggctac ttccgcacct ttgaagaaaa atttgacggt
     6721 caggttcgcg gctaccacaa accgattatg attgccggcg gtctgggcag cattcaggcg
     6781 cagcagacgc ataaagacga aatccccgaa ggcgcattgc tgattcaact gggcggcccg
     6841 ggcatgctta tcggcttggg cggcggcgcg gcttcttcga tggataccgg cacgaacgac
     6901 gcgtctttag acttcaattc cgtacaacgc ggcaaccccg aaatcgaacg ccgcgcgcag
     6961 gaagtcatcg accgctgctg gcagctcggc ggcaaaaacc cgattatctc catccacgac
     7021 gtgggcgcgg gcggtttgtc caacgccttc cccgaactcg tcaacgatgc cggacgcggc
     7081 gcggtattca agctgcgcga agtgccgctg gaagaacacg gtttaacgcc tttgcaaatc
     7141 tggtgtaacg aatcgcaaga gcgttatgtg ttgtcgattt tggaaaaaga tttggatacc
     7201 ttccgcgcca tctgcgaacg cgaacgctgc ccgtttgccg tagtcggcac ggcgaccgac
     7261 gacggtcatt tgaaagtgcg cgacgatttg ttcgccaaca accccatcga tttgccgctg
     7321 aacgtcttgc tcggcaaacc gcccaaaacc acgcgcagcg acaaaacggt tacgccatct
     7381 aaaaaaccgt ttcacgccga cgatatcgac atcaccgaag ccgcctaccg cgttttgcgc
     7441 ctgcctgccg tagccgccaa aaacttcctg attaccatcg gcgaccgcag cgtcggcggc
     7501 atgacccacc gcgaccaaat ggtcggcaaa taccaaaccc ccgtagccga ctgcgccgtt
     7561 accatgatgg gcttcaatac ctatcgcggc gaagcaatgt ctatgggcga aaaaccgacc
     7621 gttgccctgt ttgacgcgcc cgcctcgggc agaatgtgcg tcggcgaagc catcaccaac
     7681 atcgcggcgg tcaacatcgg cgacatcggc aatatcaaac tctccgccaa ctggatggcg
     7741 gcgtgcggca acgaaggcga agacgaaaaa ctctaccgca ccgtcgaagc ggtttccaaa
     7801 gcctgtcagg cattggattt gagcattccc gtgggcaaag acagcctgtc gatgaaaacc
     7861 gtatggcagg atggcgaaga gaaaaaatcc gtcgtttcgc cgttgagcct gattatctcc
     7921 gcgttcgcgc cggttcaaga cgtacgcaaa accgttacgc ccgaattgaa aaacgtcgag
     7981 gacagcgtat tgctgtttgt cgatttgggc ttcggcaaag cgcgtatggg cggctctgca
     8041 ttcggtcagg tatacaacaa catgagcggc gacgcgcccg atttggacga cacaggccgt
     8101 ctgaaagcgt tttacaacgt gattcaacag cttgtcgccg aagacaaact cttggcatac
     8161 cacgaccgca gcgacggcgg cttgtttgcc gttttggtag aaatggcgtt tgcggggcgg
     8221 tgcggcttgg atatagattt aaatttattg cttgcacaaa catttattac caaccatacc
     8281 gctctgtctc aatcattgcg gactgaagag gtaaaagcgt tggctgaatg gcaagaaacc
     8341 attgcccgca cattatttaa tgaagagttg ggtgctgtta tccaagttag aaaacaagat
     8401 gttgccgata ttatcaattt attctatcaa caacagctgc atcataatgt ctttgaaatc
     8461 ggtacgttaa ctgatgagaa cacgttaatc atccgcgacg ggcaaacgca ccttatttct
     8521 gacaacctaa tcaaactgca acaaacctgg caagaaacca gccatcaaat ccaacgcctg
     8581 cgcgacaacc ctgcctgcgc agacagcgag ttcgccctga ttggcgacaa cgaccgcagc
     8641 gcattgtttg ccgacgtgaa atttgacgtg aacgaagaca tcgccgcgcc tttcgtcaac
     8701 agcggcgcga aacccaaaat cgccatcctg cgcgaacagg gcgtaaacgg acaaatcgaa
     8761 atggccgccg ccttcacccg cgccggtttc gatgcctacg acgtgcatat gtccgacctg
     8821 atggcgggcc gcgtcaacct tgccgacttc aaaatgctgg cggcgtgcgg cggcttcagc
     8881 tacggcgacg tacttggtgc gggcgaaggc tgggcaaaat cgattctgtt ccaccctgct
     8941 ctgcgcgacc aatttgccgc tttctttgcc gatccgaaca cgctgacatt gggcgtgtgc
     9001 aacggctgtc aaatggtcag caaccttgcc gagattatcc cgggcgcaga agcatggcct
     9061 aaattcaaac gcaacttgag cgaacagttt gaagcgcgcc taagcatggt tcacgtccca
     9121 aaatcagcct ccctgattct gaacgaaatg caaggctcca gcctgcccgt cgtcgtcagc
     9181 cacggcgaag gccgcgccga cttcgcgctt cacggcggca aaatttcaga cgacctcggc
     9241 atcgcgctgc aatacgtcga cggacaaaac caagttaccc aaacctaccc gctcaacccc
     9301 aacggctcgc cgcagggcat cgccggcgtt accaacgccg acggccgcgt taccatcatg
     9361 atgccccacc ccgaacgcgt gtaccgcgct gcacaaatga gctggaaacc ggaagactgg
     9421 acggaactct ccggctggta ccgcctcttt gccggcgcac gtaaagcctt gggctaaccg
     9481 ccctactcaa accaatgccg tctgaagaat atttcagacg gcgttccggc ataccatcct
     9541 ttaaacggta tccgtccacc gaggaacact catgaaaatc acacccgtca aagccctaac
     9601 cgacaactac atctggatga tacagcacgg caaccatgcc gtctgcgtcg acccttccga
     9661 accctcgccc gtcttggaat tcctcgtccg caaccgcctc atgcttgccc aaacatgggt
     9721 aacgcacccc caccccgacc acgagggcgg tgcggcggca ctctggcgcg gctacatgga
     9781 atcgcccgtt tacggcgaat ccgacatcga agccgccacc cacaccgtaa ccgccggcac
     9841 ccaattcacc ttcggcgacg gacaggttac cgtttgggca acacccggcc acacagaccg
     9901 ccacaccagc taccttctcg aaacttcaga cggcatacac gtcttttgcg gcgacaccct
     9961 tttttccgcc ggctgcggac gcgtgtttac cggcacaatc gaacagcttt acgacagctt
    10021 ccaacgcttc aaccgcctgc ctgaaaacac cctgttctat ccggcgcacg aatacaccgc
    10081 cgccaacctg gattttgccg cccatatcga gccggacaac gccgacattc agacggcact
    10141 gaaagcggcg gcgcatacgc ctaccctgcc cgttaccctc gcgcacgaac gccgtgtcaa
    10201 tccgtttttg cgcgtcgacc tgccgcacgt cagagaccgc gccgaggcat tgagcgggaa
    10261 aacgttaaac agcagcctcg atacctttgt cgcgctgcgt gaacttaaaa accaataccg
    10321 gacgaaataa aacaacggga aaacgcagcc attcctagga tttttattaa aatcttaaat
    10381 aaaatcatac aatcatcgcc aatagacgaa aggacaccgt tgccttataa tcaaacaaaa
    10441 acaaaatata taatatagtg gattgaattt aaatcaggac aaggcgacga agccgcagac
    10501 aatacagata gtacggcaag gcgaggcaac gctgtactgg tttttgttaa tccactatat
    10561 tgttaatcca ctatataaat ccagcacaaa acgggatcgg tgattcttgt ccgcaagaat
    10621 cgttgatttt ctctattaca cggataatca tcatgcgctt cacacacacc accccatttt
    10681 gttccgtatt gtccaccctc ggtctttttg ccgtttcccc tgcttactca tccattgtcc
    10741 gcaacgatgt cgattaccaa tattttcgcg actttgccga aaataaaggc gcgttcgccg
    10801 taggtgcaag caatatttcc atccaagaca agcaaggcaa aatattaggc agggttctca
    10861 acggcatccc catgcccgac ttccgagtca gcaaccggca aaccgccatc gccaccctga
    10921 ttcaccccca atacgtcaac agtgtcaaac acaacgtcgg ctacggttcc atacaattcg
    10981 gcaacgacac ccaaaatcca gaagaacaag cctataccta ccgcctcgta tcacgcaacc
    11041 cgcacccgga ctacgactac caccttcccc gcctcaacaa actggttacc gaaatctcac
    11101 ctaccgcact cagcagcgta cccttgcttg gaaacggcca gccaaaggcc aatgcctacc
    11161 tcgataccga ccgcttcccc tactttgtac gactcggctc aggcacgcaa caagtccgca
    11221 aagcagacgg cacgcgtaca cgaaccgccc cggcatacca atacctgacc ggcggcacgc
    11281 cgctgaaagt attggggttc caaaaccacg gcttactcgt cggcggcagc ctgaccgacc
    11341 aaccccttaa cacctacgca atcgccggag acagcggttc ccccctgttt gccttcgaca
    11401 agcatgaaaa ccgctgggtg cttgcgggcg tactcagcac ctacgccggc ttcgataact
    11461 tcttcaacaa atacatcgtc acgcaacccg aattcatccg ttccaccatc cgccaatacg
    11521 aaacccggct ggatgtcggg ctgaccacca acgaactcat atggcgcgac aacggtaatg
    11581 gcgacagcac cctgcaaggg ctcaacgaac gcatcaccct gcccattgca aacccttcgc
    11641 ttgccccaca aaacgacagc aggcacatgc cgtctgaaga tgccggcaaa acgctcatcc
    11701 tatccagcag gttcgacaac aaaacactga tgctggcaga caatatcaac caaggcgcag
    11761 gcgcattgca gttcgacagc aacttcaccg tcgtcggtaa aaaccacaca tggcaaggtg
    11821 caggcgttat cgtagccgac ggcaaacgcg tcttctggca agtcagcaac cccaaaggcg
    11881 accggctctc caaactgggc gcaggcacgc ttatcgccaa cggacaaggc atcaaccagg
    11941 gcgacatcag catcggggaa ggcactgtcg tactcgccca aaaagctgct tcagacggca
    12001 gcaaacaagc attcaaccaa gtcggcatca ccagcggcag gggcacggcc gtcctcgccg
    12061 acagccagca aatcaaaccc gaaaacctct atttcggctt caggggcgga cggctcgacc
    12121 tcaacggcaa caaccttgcc tttacccata tccgccatgc ggacggcggc gcgcaaatcg
    12181 tcaatcacaa ccctgaccaa gccgcgacac tgacgctgac cggcaacccc gtcctcagtc
    12241 ccgagcatgt cgagtgggtg aaatggggca accgtccaca aggcaacgcg gcggtttacg
    12301 aatacatcaa cacgcaccgc aaccgtcgga ccgactactt catactcaaa cccggcggca
    12361 acccgcgcga atttttcccg ttaaatatgg aaaactcaac aagctggcaa tttgtcggca
    12421 acaacaggca acaggccgcc gaacaagtcg cccaagccga aaatgcccgc cccgacctga
    12481 ttaccttcgg cggatacttg ggtgagaacg cgcaaacggg taaagccgca ccgagttaca
    12541 gcaaaaccaa tatcgaatgg gtgaatagtc caaacacccc acacaagccc tcgccctata
    12601 cgaatacgcc aacccgcaca ggggcaggcg taccgacgat taccgcctca aagaaggcgg
    12661 caaccccggc gcctacttcc catgggatgc ctacagcacc caagactggg aatttctcgg
    12721 acataaccgg cgaagcagcc atagaaaaaa cccgccatat cgcaaatgcc gccgtatacg
    12781 gccggcccga ataccgttac aacggcgcac tcaacctgca ctatcgtccc aaacgcaccg
    12841 acagcacgct gttgctcaac ggcggcatga accttaacgg ggaagtcttg attgagggcg
    12901 gcaatatgat tgtgtcaggc aggcccgtac cccatgccta cgaccaccag gccaaacgcg
    12961 aacccgttct tgaaaacgaa tggaccgacg gcagcttcaa ggctgcacgg ttcaccctgc
    13021 gaaaccatgc ccgactgacg gcagggcgca ataccgcgca tctggacggc gacataaccg
    13081 catacgatct gtccggcatc gacctcggct ttatccaagg caaaacaccg gaatgctacc
    13141 gctcctacca tagcggcagc acccactgca cacccaacgc cgttttaaaa gccgaaaact
    13201 atcgtgcact acctgcaacg caagtacgcg gcgacattac ccttaacgac cgttcagagc
    13261 tccgcctggg caaagcacac ctgtacggca gcatccgtgc cggcaaagac accgcagtcc
    13321 gcatggaagc agacagcaac tggacacttt cccagtccag ccacaccggc gcactgacgc
    13381 ttgacggcgc acaaattacc ctgaaccccg atttcgccaa taatacacac aacaaccgct
    13441 tcaacacact gaccgtcaac ggcacacttg acgggttcgg cacattccga ttcctgaccg
    13501 gcatcgtccg aaaacaaaat gccccccccc tcaaactgga aggggacagc cgcggcgcat
    13561 tccaaatcca cgtcaaaaac accggacaag aacctcaaac aaccgaatcg cttgcacttg
    13621 tgagcctcaa tccgaaacac agccaccaag cccgattcac cctccaaaac ggctatgccg
    13681 atttgggtgc ctaccgctac atcctccgca aaaacaacaa cggatacagc ctgtacaacc
    13741 cgctcaaaga ggccgaactt caaattgaag ccacgcgtgc ggaacatgag cgcaaccaac
    13801 aggcatacaa ccaattacag gcaaccgaca tcagcagaca ggttcaacat gactctgacg
    13861 cgaccaggca ggcactacag gcctggcaga acagtcaaac cgaacttgcc cgcatcgaca
    13921 gccaagtcca atatctgtcc gcccaattga aacagacaga cccgctaacc ggcattctga
    13981 cgcgtgccca aaacctgtgt gccgcacaag gatacagtgt cgatatctgc cgtcaggttg
    14041 ccaaagccgc cgacacgaac gacctgacac tcttcgaaac cgaactggat acgtatatag
    14101 aacgtgtaga aatggccgaa tccgaacttg acaaagcacg gcaaggcggc gatgcgcaag
    14161 ccgtcgaaac agcccggcac gcctacctga acgcactcaa ccgtctgtcc cgacaaatcc
    14221 acagtttgaa aaccggcgtt gccggcatcc gtatgccgaa cctggccgaa ctgatcagcc
    14281 ggtcggccaa caccgccgtt tccgaacagg ccgcctacaa taccggccgg caacaggcgg
    14341 gacgccgcat cgaccgccac cttaccgatc cgcagcagca aaacatctgg ctggaaaccg
    14401 gtacgcaaca aaccgactac catagcggca cacaccgtcc ctaccaacaa actaccaact
    14461 atgcacatat cggcatccaa accggcatca ccgaccgtct cagtgtcggt acgattttaa
    14521 ccgatgagcg cacaaacaac cgttttgatg aaggcgtatc cgcccgaaac cgcagcaacg
    14581 gcgcacatct gttcgtcaaa ggggaaaacg gcgcactctt tgccgcggca gatttaggct
    14641 acagcaacag ccgtacccga tttaccgatt atgacggggc tgccgtccgc cgccacgcat
    14701 gggatgcagg catcaacacc ggcatcaaaa tcgataccgg catcaaaatc gataccggca
    14761 tcaacctcag accctatgcc ggcatccgta taaaccgcag caacggcaac cggtacgtac
    14821 tcgacggcgc agagataaac agcccggcgc aaatccaaac cacatggcat gccggcatcc
    14881 gtctcgataa aaccgtcgaa ctgggtcaag ccaagctgac ccccgctttc agcagcgatt
    14941 actaccatac ccgccaaaac agcggttccg ccctcagcgt caacgaccgt accttactgc
    15001 agcaagccgc ccacggcaca ctgcataccc tgcaaatcga cgccggatac aaaggctgga
    15061 acgccaaact tcatgccgct tacggcaaag acagcaacac cgcccgccac aaacaggcag
    15121 gaatcaaaat aggctacaac tggtaacaag ccgataaaaa tgccgtctga aacccgcgtt
    15181 tcagacggca tttgcgttaa aaatagtaaa ccgttccaaa agggagtaga atagtgccgt
    15241 ttccaaccct gcgcctgtac cgtcaggctt ttattatgga ccttcccagt tcgcttttac
    15301 tgaacgcccc ttccgattcc aaacgacaat aaccatcccg ccggaatgcc tccccgcaca
    15361 cggcgggcgg agcatttatg agcatcgaac acactcctcc cacacacgac ggcgaaaccg
    15421 gtcaaaacca tgccgaacgc ccttccgccg atttcgaccg tgtccactcc ctctgcgaaa
    15481 tcctcgaacc tgcttttgaa caaatcgaaa acggtacacc gcttgaagac gcgccgctgc
    15541 gcgacaagct gaccgagctg accgtcctct tgagcgagct gcaccctgcc gacgtggcgg
    15601 cggtgttgga atcgctgccg ccgcgcgaac gcaacgtcgt ttggcttctg gtcaaacccg
    15661 aagacgacgg cgaagtattg ctggaagtat ccgacgcggt gcgcgaaaca ctgatcgagt
    15721 cgatggataa ggacgaattg ttggcggcag ttgacgattt ggacgcggac gaattggcgg
    15781 aactggcaga cgatttgccg caccaagtgg tttatgaagc cttgcagacg cgcgacgagg
    15841 aagagcgcgc gcaagtcaaa gcggcaatgt cctacgaaga caaccaagtc ggtgcgatta
    15901 tggacttcga gttggtcagc atccgcgccg atgtcgcctg tgaagtggtg ctgcgttacc
    15961 tgcgccgctt cgacagcctg cccgaccata ccgacaagat ttttgtggtc gatgaaaacg
    16021 atgtgttgca gggcgtgctg cccatccgca aacttttggt cgccgatccc gaagacttgg
    16081 tggaaaacgt gatggcgaaa gatgtcgtgc gtttccgcgc cgaagacgac gtggaagaag
    16141 cggcgcaggc gtttgaacgc tacgacttgg ttaccgcgcc cgtcgtcgat gaaaacaaaa
    16201 agctcatcgg taggattacc gttgacgaga tggtggacgt catccgcgaa gaatccgaag
    16261 ccgatatgtt gaacatggca ggtttgcagg aagaggaaga cctgttcgcc cccgtatggg
    16321 attcggtgaa aaaccgctgg atgtggctcg ccgtcaacct ctgcaccgcc ttcctcgcca
    16381 gccgcgtcat cggcgcgttt gaaggcagca tcgaaaaaat cgtcgcactc gccgcgctga
    16441 tgcccatcgt cgccggtatc ggcggcaact cgggcaacca gacgattacc atgattgtcc
    16501 gcgcaatggc gatggggcag ttgacgggaa tgcaggcggg gcgcttgttg aaaaaagaag
    16561 tcggcgttgc cctagtcaac ggcattatct ggggaaccgt catgggcgcg gtttcatggc
    16621 tgctctacgg cagcctcggc atcgggctgg ttatgattgc cgcgatgacg ctcaacctcc
    16681 tgctggcggc aaccgtcggc gtattaattc ccgtggtaat ggaaaagttc ggacgcgatc
    16741 ccgcactggg cagctcggtg ctgattaccg ccgttaccga ctccggcggc ttcctgattt
    16801 tcttggggct cgccacccta ttcctgcttt aaatgccgtc tgaacccgcg caaaaatgcc
    16861 gtctgaagcg gaagctgctt cagacggcat ttgactattt atccttgttg cacaagatta
    16921 ttggacggta tgccggggca gccctttggc aacgccgacc acatcctccc cgaacagcgc
    16981 gttgacatcg gtttcgtcaa acacatattt gctgtggcag aaatcgcaat cgacttcgat
    17041 gctgccttgt tccaccacca cgccgccgac ttcttccccg cccagcatca acagcatatc
    17101 gctgactttg ccgcgcgaac aggtgcatga aaattcaaac gtttccggct cgaacacgcg
    17161 cggcggcgtt tcgtggaaca ggcggtacaa aacatgttgc gcatccaatt ctgccagctc
    17221 gtccgccgtc agtgtacgcg ccagcgtact gacgtgttcc catgcctctt catccaacac
    17281 cgcttcaggc agacgctgca ccagcagacc gcccgccgct tcgtcgcttg cagacaggac
    17341 gatgtgcgta tcgatttgtt cggaacgctt catatagttc gccaacattt gcgcgatacc
    17401 gccgccttcc aaaggcacta cgccctgcca gggttcgccg tctttgggct gcagcgtcag
    17461 cacgaatacg ccgccctcgc ccaaaaggtc gccgaggctt tcgtcatcgg ctatttctgc
    17521 ggtttcgtcc caacgcgcgg ttgcacggac ggtacggtcg gaagccgctt ccgcaaccag
    17581 cattttcagc cgcccccgcc cctgaacctg cacaatcagc gtgccttcgt ttttgaggtt
    17641 gcccgacagc aacacacccg ccgccaacaa ctcacccaaa gcgcggcgga tggcggcggg
    17701 atagtttttc tgttttacaa tgtgctgcca cacgttttcc agacggacgt gcagcccgcg
    17761 cacgggcata tcgtcgaaga taaagcgggt acgcacatcg gcgcggttga tggcggtttg
    17821 attcatgatt ttctctgact gattgttcgg atggcggcta tatggttgcg gtcggcgcga
    17881 aaacaagacg gacggcggat gcgcttccca aattatcaat aaattatata aaaatcaaca
    17941 tattaactca atctaacaag ccgttttttg ccaaacagcc gtttttttat atacaatcaa
    18001 caagatattt tcgactgata cagcataaca tcgcacggcg gcacgatgcc tcctgcgcgg
    18061 aaacaccgat atggattctt ttttcaaacc ggcagtttgg gcggttttgt ggctgatgtt
    18121 tgccgtccgc cccgcccttg ccgacgagtt gaccaacctg ctcagcagcc gcgagcagat
    18181 tctcagacag tttgccgaag acgaacagcc cgttttaccc atcaaccgag cccccgcccg
    18241 gcgggcgggc aatgccgacg aactcatcgg cagcgcgatg gggcttaacg aacagcccgt
    18301 tttacccgtc aaccgagtcc ccgcccggcg ggcgggcaat gccgacgaac tcatcggcaa
    18361 cgcgatgggg cttaacgaac agcccgtttt acccgtcaac cgagcccccg cccggcgggc
    18421 gggcaatgcc gacgaactca tcggcaacgc gatgggactt ttgggtattg cctaccgcta
    18481 cggcggcaca tcggtttcta ccggttttga ctgcagcggc ttcatgcagc acatcttcaa
    18541 acgcgccatg ggcatcaacc tgccgcgcac gtcggcagaa caggcacgga tgggtacgcc
    18601 ggttgcccga agcgaattgc agcccggaga tatggtgttt ttccgcacgc tcggcggcag
    18661 ccgcatttcc catgtcggac tttatatcgg caacaaccgc ttcatccacg cgccgcgcac
    18721 ggggaaaaat atcgaaatca ccagcctgag ccacaaatat tggagcggca aatacgcgtt
    18781 cgcccgccgg gtcaagaaaa acgacccgtc ccgctttctg aactgatttc ccaaggaata
    18841 cgcaatgagt atgcccgaaa tgcccaaatg gtacgacgat gacggacaga tcgtgtcctg
    18901 taccgaaaag gtcaaagtga tgtccgaaaa tatggccgag ctgtatcaga cggcacaaga
    18961 cgcgtttgaa gacgcgctgc tgatgggttg cggcgaacgt cagttgcgcg attacctgct
    19021 cgcgctgatt gaaggtttgg aaaatcccta ccgcaaagtc tgaacacgcc ccggttgctg
    19081 cggcacggtt tatccgtgcc gtttttgcgt ttgtgcgcgg cttcggcttt tcagacggca
    19141 tatttgacgt tatgattaaa cagttaacaa gatttatcac aacgccgtca aaagacagac
    19201 acacaacatg aacatcatcc gcgcgctcct catcatcctc ggctgcctcg ccgccggcga
    19261 aaccgccgtt ttcctagcag gcatcaaact gcccggcagc atcgtcggca tgggcgtgct
    19321 gtttgcgctt ttgcaggcag gttgggtcaa aacgtcttgg ctacaacagc ttaccgacgc
    19381 gctgatggcg aatctgacgc tgtttctcgt gccgccctgc gtggcggtca tcagctattt
    19441 ggatttgatt gccgacgatt ggttttcgat actggtttcc gcctccgcca gcactttgtg
    19501 cgtactgctg gttacaggca aggttcaccg ctggatacgg agcattatct gatgaacgaa
    19561 atcctcaggc agcccagcgt tctgcttttc ctcacgcttg ccgtgtacgc gcttgcgatt
    19621 atcgtgcgca cgcgcacggg caatatcttc tgcaaccccg tactcgtcag cactatcgtg
    19681 ctgattgcct acctgaaaat cctcgacatc gattatgcgg tgtaccacaa cgccgcgcaa
    19741 ttcattgatt tttggctgaa acccgccgtc gtcgtgcttg ccgtgccgct ctaccaaaac
    19801 cgccgtaaaa tcttcaacca gtggctgccc gtcatcgttt cacagcttgc gggcagcgtt
    19861 acgggcattg ttacggggat gtattttgcc aaatggctgg gcgcggaacg cgaagtcgtc
    19921 ctctcgctcg cgtccaaatc tgttaccaac cccatcgcca ttgaaatcac ccgctccatc
    19981 ggcggcattc ccgccattac cgccgccacc gtcatcattg ccggtctggt cggacagatt
    20041 gccggttaca aaatgctgaa gaacacagtc gtcatgccct cgtccgtggg tatgtcgctc
    20101 ggcacggctt cgcacgcgat ggggattgcc gcctcgctcg aacgcagccg ccgcatggcg
    20161 gcatacgcgg gactggggct gacgttcaac ggcgtactga ccgcgctgat tgcgccgctg
    20221 cttatccccg ttttgggatt ttgaacccgt ttcagacggc atttcagccc atgctgtctg
    20281 aacgccgaca cactcgcaag gagaaccgtt atggctgtca acctgactga aaaaaccgcc
    20341 gaacaactgc ccgacatcga cggcattgcc ctctacaccg cccaagcagg cgtgaagaag
    20401 cccgggcata ccgacctgac actgattgcc gtagccgccg gcagcaccgt cggtgcagtc
    20461 ttcacgacca accgtttctg tgccgcgccc gtccacatcg ccaaatcgca ccttttcgac
    20521 gaagacggcg tgcgcgccct catcatcaac acgggcaacg ccaacgcggg tacgggcgca
    20581 cagggcagaa tcgatgcttt ggcagtgtgt gccgccgccg cccggcaaat cggctgcaaa
    20641 ccgaaccaag tgctgccctt ctccaccggc gtgattctcg aaccgctgcc cgcagacaaa
    20701 atcatcgccg cgctgcccaa aatgcagcct gccttctgga acgaagcggc acgcgccatc
    20761 atgaccaccg acaccgtgcc caaagccgcc tcgcgcgaag gcaaggtcgg cgacaaacac
    20821 accgtccgcg ccacgggcat cgccaaaggc tcgggcatga ttcatcccaa tatggcgacc
    20881 atgctcagtt tcatcgccac cgatgccaaa gtttcccaac ccgtcctcca actgatgacg
    20941 caggaaatcg ccgacgaaac cttcaacacc atcaccgttg acggcgacac cagcaccaac
    21001 gacagcttcg tcatcatcgc caccggcaaa aacagccaaa gcgaaatcga caacatcgcc
    21061 gacccgcgtt acgcccaact caaagaattg ttgtgcagcc tcgcgctcga actcgcccaa
    21121 gccatcgtcc gcgacggcga aggtgcgacc aagttcatca ccgtccgcgt cgaaaacgcc
    21181 aaaacccgcg acgaagcccg ccaagccgcc tacgccgtgg cacgttcgcc gctggtcaaa
    21241 accgcctttt tcgcctccga ccccaacctc ggcaggctgc tcgccgccat cggttatgcc
    21301 ggcgttgtcg acctcgatac tgacctcgtg gaaatgtatc tcgacgatat tttggttgcc
    21361 gaacacggcg gacgcgccgc aagctacacc gaagcacaag ggcaggcggt gatgtcgaag
    21421 gccgaaatca ccgtccgcat caagctgcat cgcggacaaa gtgccgccac cgtctatacc
    21481 tgcgacctgt cgcacggata cgtttccatc aacgccgact accgttcctg acccgacacg
    21541 gcttcagacg gcatacataa aatgccgtct gaaccgccgg acaacatgcc atgacctcca
    21601 cattcccccg ccgcctcgcc cgcaaaatcc gccaaacccg ccgcctgtcg cgcaaaagca
    21661 tcgcctttct gttccttttg gcaggttcgg cactcgtcgc cctgaccgcg ctgttttttg
    21721 cccatcttgc cgattttgcg ctggaactga acgccaaact ggttcaacaa tacccgtggt
    21781 tcgcgtgggt cgcgcttcct ttgggtttgc cgcttattgc gtggctcaca cgcaaattcg
    21841 cccccttcac cgccggcagc ggcatcccgc aggtcatcgc ctcactgtcg ctgccctacg
    21901 gcgcacagaa aacgcggctg atccgcctcg ggcagacgct gctgaagatt ccgctgacct
    21961 ttttgggtat gctgttcggc gcgtccatcg gacgcgaagg tccgtccgtg caggtcggcg
    22021 cggcagtgat gggcgcgtgg ggcgcgtggt gcaagaaaca cggcttggca ttcaaaggga
    22081 tgcaggaaaa cgatttgatg gcggcgggcg cggcgggcgg tttggcagcc gcgttcaacg
    22141 cgccgctggc gggcgtgatt ttcgccattg aggaactcgg gcgcggcatc atgttgcgct
    22201 gggagaggca aattcttttg ggcgtgctcg cctccggttt catacaggtc gccattcagg
    22261 gcaacaaccc gtatttttcc ggcttcaacg gcggcgtatt ggaacatatc tttctgtggg
    22321 tcgcactgtc cggcctggtt tgcggcgcgg cgggcgggct gttcggacgt ttgctctatc
    22381 gcggtgcggc ggcatttgca ccacgcaaga tacgcggctt catccgcaac cgtccgctgc
    22441 tgctggcggc actgatgggg ctgctgctcg ccctgctcgg cacgttctac caaggcaaaa
    22501 cctacggcac cggctaccac gaagccgccc aagccctgca cggcatctat gaagccccct
    22561 tcggactcgc cgccgccaaa tggctcgcca ccgtattcag ctattgggca ggcgttccgg
    22621 gcggcatttt cactccctcg ctgaccatag gcgcggtttt gggcgagcat atcgccgcca
    22681 tcgccgacat atcgcagggt gcaaacatca tcgtcctcat ctgcatggcg gcatttctgg
    22741 cgggcgcgac acaatccccg attacttccg ccgtcgtcgt catggaaatg acgggcggac
    22801 aaagcctgct gttttggatg ctaattgcct gcattttcgc ctcgcaggtt tcgcgccagt
    22861 tttcgccgcg tccgttctac cacgcatcgg gaatgcgctt ccgccagcgc gtgcttcagg
    22921 aaaccaccgc ccaaaccggc aatgcgcccg caagaccgca aacagcaaac agcaaaacgg
    22981 gaatgccgtc tgaaaattaa aacgcccccg atcaaacgcc ggcagccgcc ttgatttgaa
    23041 taccgttccg ccgccgcttg aaatttcagc aacaatgccg tctgaacgac agaatgcggt
    23101 tttcagacgg catttccaca tcccgatatt gcctaaacaa aaccgaagcg tttgctataa
    23161 ttctattttt taccgcacac gcaccaatca tgtttcccga tttctcccaa accctctcca
    23221 aagaccgcca cttcctacgt tccgccttca aaaatcccaa caaatacggc gggttgtcca
    23281 aagtcgaaga aaaataccga aaatcgcacg aaatcttttt gaaacgtttg gcagccttgc
    23341 ccaaacccga attcgacaac accctgcccg ttcacgaaaa acttgacgaa atcaaaaaaa
    23401 ccgttgccga acatcaggta acgattattt gcggcgaaac cggttcgggc aagaccactc
    23461 agttgcccaa gatttgtctg gaactcgggc gcggggcggc gggtttgatc gggcataccc
    23521 agccgcgccg tttggcggcg cgttccgtag cagagcggat tgccgaagag ctgaaatccg
    23581 aaatcggcag cgcggtcggc tataaagtgc gcttcaccga ccacacctcg cgcgatgcct
    23641 gcgtcaagct gatgaccgac ggcatcctgc tggcggaaac ccagaccgac cgttatctcg
    23701 ccgcctacga cacgattatc atcgacgaag cgcacgagcg cagcctgaac atcgacttcc
    23761 ttttgggcta tttgaaacaa ctcctgccgc gccgccccga tttgaaagtc atcatcacct
    23821 cggcaacgat agacgcagaa cgcttttccc aacacttcaa cggcgcgccc gtgctggaag
    23881 tgagcggacg cacctatccc gtcgaaatcc tctaccgacc gctgatcggc aaagacgaag
    23941 acgacgcgga aatagaattg accgacgcga ttgtcgatgc ggcggacgaa ttggcgcgat
    24001 atggcgaagg cgatattttg gtattcctgc cgggcgaacg cgaaatccgc gaagccgccg
    24061 aagccctgcg caaatccacg ctacgccgca acgacgaaat cctgcccctg ttcgcacgcc
    24121 tgtcgcacgc cgagcagcac aaaatctttc acccctccgg agcgaaacgc cgcatcgtgc
    24181 tggcaaccaa cgtcgccgaa acctcgctta ccgtgccggg catcaaatac gtcatcgaca
    24241 ccggcctcgc gcgcgtcaaa cgctattccg cacgggcgaa agtggagcag cttcatatcg
    24301 aaaaaatctc ccaagccgcc gcccgccaac gctccggccg ctgcggacgc gtctccgcag
    24361 gcgtatgtat ccaattgttt tcagaagaaa atttcaacaa ccgccccgaa tttaccgacc
    24421 ccgaaatcgc ccgcagcaac ctcgccgccg tcatcctgcg catggcatcg ctgaaactcg
    24481 gcgacgtagc ggcattccca tttttagaaa tgcccgattc gaggtatatt aatgatgggt
    24541 ttcaggtgtt gctggaatta ggggcagtgg aggccgcctg aaaacaagta ggatatacac
    24601 ttctacctga caaagaccaa caaacgataa aactacggca catctagatg cccaacctac
    24661 aacattgcac acggtaaaaa catgaaacac ttactcatcg attttgaaaa cgtccagccg
    24721 caaaacttag acaaattact aaccgaaaat acccatattt ggctatttat aggtgtatta
    24781 cacaaaatgt tacctattag tctggtgcaa tccctactac gtttcggcga acgtgtccat
    24841 cttgtccagt tacaaaaaac ggggaaaaac gcattggatt tttacctgtc ctattacctc
    24901 ggacaaatta ccgccacaga ccccaatgcc caaatcggca tactctcgcg tgatggagga
    24961 tacgatgttc tggtcgaaca tattttgaaa aaccaccagg cgaagggtat cgtgcgccta
    25021 gccaatatag atgaagtaca acatcagaaa attactaccg aaccgccgtc agcattgctg
    25081 gaaaacactc ctcagcctga aaccaccctc aaaccacagc aaccattaac ttcctatttc
    25141 caagcagccc taactgcact gcgccgcccc gacgctttcc gcccctgccg cctgcataac
    25201 ctgcgacaaa atctgcgtaa gcatattttg agtgatttgt ttaaagaaaa aaccgatgaa
    25261 gaatgcgaaa taaccactgc taacgttatc aataaactca aagcacaaaa cttcatcagc
    25321 attgatgaac aggaaaccgt ttcctaccat ctcagtgata atgatttgtt acaaagaatc
    25381 caacgccata ttttaagcca acgtcccaaa acctacgctg attttcaagc cgtcgtgcaa
    25441 aaccgagcag atgcacttca cttaacagtc ggtaccaacg acattcaatc ctttgcgcga
    25501 catttgcgcg accaaaacct gatccgccaa aacaatggga aaattgaata tgcaccgttt
    25561 actgaaccta aaccacagcc aacgcccaag cagcctaaaa aaaccgcatg ggaacctgat
    25621 gaaattattt ggaaaaaagt gattgccgcg ttatcgttaa agaaccgtcc taataaaacc
    25681 aaaactttac gcaatacaat ccaggcactc acaaaatcca atgcacaaga aactgacaaa
    25741 ctgctacaac atttacaaga tacccaagtc ctacgtattg acggtactaa aattgtgtat
    25801 atcaagtagg atgggcattt atgcctgacc tacaaaacca aaaacaacct aacaccctta
    25861 atcaactcca caaacccttt tcagacaacc tcgttttttg aaaaacaatc tgtaaacaga
    25921 taactgctga agaataccgt tgccgagccc caaaacctgc ctgcaacttt tattgtgaac
    25981 tgcccattat gaaaaaatcc cttttcgttc tctttctcta ttcatctcta cttaccgcca
    26041 gcgaaatcgc ctatcgcttt gtattcggaa ttgaaacctt accggctgca aaaatggcag
    26101 aaacgtttgc gctgacattt gtgattgctg cgctgtatct gtttgcgcgt tataaaactt
    26161 cgcggctgct gattgcgctg tttttcgcgt tcagcattat tgccaacaat gtacattatg
    26221 cggtttatca aagttggatg acgggtatta actattggct gatgctgaaa gaggttaccg
    26281 aagtcggcag tgcgggcgcg tcgatgttgg ataagttgtg gctgcctgcg ttgtggggcg
    26341 tggcggaagt cgtgctgttt tgtagccttg ccaagttccg ccataagacg catttttctg
    26401 ccgatatatt gtttgccttc ctaatgctga tgattttcgt gcgttcgttc gacacgaaac
    26461 aagagcacgg tatttcgccc aaaccgacat acagccgcat caaagccaat tatttcagct
    26521 tcggttattt tgtcggacgc gtgttgccgt atcagttgtt tgatttaagc aagattcctg
    26581 tgttcaaaca gcctgctcca agcagaatcg ggcaaggcag tattcaaaat atcgtcctga
    26641 ttatgggcga aagcgaaagc gcggcgcatt tgaaattgtt tggctacggg cgcgaaactt
    26701 cgccgttttt aacccggctt tcgcaagccg attttaagcc gattgtgaaa caaagttatt
    26761 ccgcaggctt tatgacggca gtatccctgc ccagtttctt taacgtcata ccgcatgcca
    26821 acggcttgga acaaatcagc ggcggcgata ccaatatgtt ccgcctcgcc aaagagcagg
    26881 gctatgaaac gtatttttac agtgctcagg ctgaaaacca aatggcaatt ttgaacttaa
    26941 tcggtaagaa atggatagac catctgattc aaccgacgca gcttggctac ggcaacggcg
    27001 acaatatgcc cgatgagaag ctgctgccgc tgttcgacaa aatcaatttg cagcagggca
    27061 ggcattttat cgtgttgcac caacgcggtt cgcacgcccc atacggcgca ttgttgcagc
    27121 ctcaagataa agtattcggc gaagccgata ttgtggataa gtacgacaac accatccaca
    27181 aaaccgacca aatgattcaa acagtattcg agcagctgca aaagcagcct gacggcaact
    27241 ggctgtttgc ctatacctct gatcatggcc agtatgtgcg ccaagatatc tacaatcaag
    27301 gcacggtgca gcccgacagc tatattgtgc cgctggtgtt gtacagcccg aataaggccg
    27361 tgcaacaggc tgccaactag gcttttgcac cttgcgagat tgccttccat cagcagcttt
    27421 caacgttcct gattcacacg ttgggctacg atatgccggt ttcaggttgt cgcgaaggct
    27481 cggtaacggg caacctgatt acgggtgatg caggcagctt gagcattcgc gatggcaagg
    27541 cggaatatgt ttatccgcaa tgagtggcgt aaaaaccaat aaagacaaat ttagatgatg
    27601 tcggggaaga tgcccgaccg acaagactat gcaaaatatg aaaaaccaaa tacgcggatc
    27661 aggcatggat gcacgatcca acccggccaa tgtttcagac ggcctgcaaa acagttcggg
    27721 ccatatcggt accaacacgc gctaccgtct gaccaaactc ggcgaacaaa tggcgcgcct
    27781 gcccatcgac ccgaaaatcg cgcgcatttt gttggcggcg aagaagcacg actgcatggc
    27841 ggaaatattg gtgattgcgt ccgcgctatc gattcaagac ccgcgcgagc ggccgctgga
    27901 ggcacgcgat gccgccgcca aggcgcatga acgctttacc gacaagcaat ccgatttcct
    27961 tgcctacctg aatatttggg acagtttcca gcgcgagcgc gacaaaggtt tgtccaacaa
    28021 gcagctggtg caatggtgcc gccaatattt cctgtcgcac ctgcggatgc gcgagtggcg
    28081 cgagctgcac caccagcttg cccaaaccgc gattgaaatg ggtttgacca ccaaggaagc
    28141 ggctttcaga cgactttccg aaatcaagca gcttacgtcg tctgaaaacc aaggcgatca
    28201 agacttatcc gccaaacgca aacaaaaaca actggataag aaacaacacc gcgcccaaat
    28261 ccgcgccgcc aaagaagcgg gctacgaaca aatccaccgc gccctgctca ccggccttat
    28321 cgccaacgtc ggcatgaaat cgcccgacgg caacgactat accggcgcgc gcggcagccg
    28381 tttccacctt ttccccgcct ccgccctgtt caaagccaaa cccaaatggg tgatggcggc
    28441 agaactcgtt gaaacgacaa aactttatgc gcgcgacgtc gccgttatcc agcccgaatg
    28501 gattgagcag gaagccccgc acctcgtccg ctaccactat ttcgagccgc actgggaaca
    28561 aaaacgcggc gaagtcgtcg ccagcgaacg cgtgacgctt tacggcctga ccgtattgcc
    28621 gcgccgcccc gtgtcttacg gcaaagttgc ccccgaagaa gcgcgcgaaa tctttatccg
    28681 cagcgcgttg gtggcgcagg aatgcgattt gaaagcggat ttttttgtcc acaacaaaaa
    28741 gctgattaaa gaaattaccg aactcgaaca caaatcgcgc aagcaagacg tgctggtcga
    28801 tgacgaagcc ctgtttgcgt tttataacga acgactgccc gaaatggctt ggaaagacgc
    28861 gcaaggcagc gtttggggaa gtgaagattc cgtacggatt attgaatctg acaaagccga
    28921 gaggtcgtct gaaaatgagc gcaacgagtt tcgtaaaaac aagcgtaatg ggtctcgcca
    28981 aaatgaaaat cacggcaaca ccgtaggttg ggttgaaaac ccaacatcag ccgcaactgc
    29041 aaaaactgtt gggtttgaca acccaaccta cgctactcag caacccaccc cctcccccga
    29101 gagggagggt cggggagagg gcaaaacagt tgccgcacaa accaactttt ccgcaaccgc
    29161 agcaaaccct ctcccgcagg agagggaaca gagtgcctca gcttcaacgt tttcagacga
    29221 cctgcgtcct gcaaatctgc agcaaaccgc cccctccccc gtgggggagg gctggggaga
    29281 gggcaaaaca gttgccacac aaaccaactt ttccgcaacc tcaacaaacc ctctcccgca
    29341 ggagagggaa cagagtgcct cagcttcaac gttttcagac gacctgcgtc ctgcaaatct
    29401 gcagcaaccc tctccctccc ccgtggggga gggctgggga gagggcaaaa cagttgccac
    29461 ccaaaccaac ttctccgcaa ccacagcaaa ccctctccct aaccctctcc cacaggaggg
    29521 ggaacagagt gccgcagctt cagcggtttc aaacgaccct caacccaaaa agcagcctgc
    29581 accccaaaaa aaccgtctga aacccctacc cctcgccgac atccgcacct tccaagcctg
    29641 gctcaaaacc gccgagcgcg acaatccgcg cctgctgttc ctcagccgcg acgatctgat
    29701 gcaacacgcc gccgcacaca ttaccgaaga acagttcccc aaattctggc aaaccgcaga
    29761 cggcaaattc aaactttcct accgcttcga gccgcaccat ccgctagacg gcgtgaccat
    29821 gaccgtgccg ctgaccgtcc tcaaccgcct gcacgcgccg tcgctcgaat ggctggtgcc
    29881 cggcatgata cgcgaaaaaa tccagttgca aatcaaagca ctgcccaagc aaatccgccg
    29941 catctgcgtg cccgtgcccg aattcatcac ccaattttta agccaaaacc ccgaccgcaa
    30001 cgcccccatc ctgccccaac tcgcccaagc catcgccaaa accgcaggcg acatccgcat
    30061 attcgagcaa atcaaccaag acgaatgggc cgcgttcagg ctgcccgaac actgctattt
    30121 caacctccgc attatcgacg acggcggaca agagcttgcc ggcggccgca aactgcacga
    30181 attgcaacaa caactcggtc aagccgccgc cgttaccttc cgcgacaaca cccaagaatt
    30241 tgagcgcgac aacgtcaccg catgggacat cggcaccctg cccgaatcca tcaaattcgc
    30301 acgcggcaaa caacagctca ccggctatct cggcctacaa aaagaaaaag acggccgcat
    30361 cgccctgcgc ctgtttgata ccacagaagc cgcagagcag gcacaccgtc aaggtgtcat
    30421 cgaattgatg aagctgcaat taaaagagca ggtaaaggat ttgaacaaag gcatccaagg
    30481 cttcacccaa gctgccatgc tgctcaaaca catcaacgcc gacactctgc gcgacgacct
    30541 cacccaagcc gtctgcgacc gcgcctttat cggcgaagac gagctgccgc gcaacgaaaa
    30601 agccttcaaa gaacaaatca aacgcgcccg cagccgcctg cccgccgtca aagaagccct
    30661 cagccgctac ctgcaggaaa ccgccgccgt ctacgccgaa ctcaacagca aactcggcaa
    30721 acacccattg acccaccttc taagactacg cctgcaaacc ctgctcgccg ccggcttcgc
    30781 cacccgaacc ccgtgggcac aatggccgcg cctccccatc tacctcaaag ccatgaccct
    30841 gcgcctcgaa aaatacagca gcaaccccgc ccgcgacgca gcccgcgaag ccgatatcca
    30901 agagctggaa caaatgtggc aggaaaaaac agacagcctg attaaacaag gtctccccat
    30961 ttcagacggc ctcgccgcgt ttaaatggat gattgaagaa ttgagggtgt cgctgttcgc
    31021 gcaggaattg aagacaccgt atccggtgtc ggtgaagcgg ctgttgaaag agtgggaaaa
    31081 aattgaaaaa taaaaaaaca gcctgaaaag tttcaggctg tttttttatt tgactaatcg
    31141 aagtttccta tatctattta agtccctctc aactaatcca aaagttaaat cagcaacatc
    31201 tttgggggat acgtttaaat tttcagcaat ctgttcaata ccaatgccat cattttttaa
    31261 aatagtaagc attttacgta atgcgcttga tatttccctt tccattggct ctggttcgat
    31321 agttcgatat tttttctttg caaacaaagg acaaagattg tgtatataca tcctatcagt
    31381 aatcattcct aatttatgca tccgatatgc taaggcaaca agtgatacac caaatcgtct
    31441 tttgattttt aataaatttt caatagtgat aggaacatga cgatataagc gtagtgcagc
    31501 ctccggcatt aaaaaagctg aagcaaaggc attagcctct ttttcgataa tatcacgagg
    31561 ttcatcttct gtaatttcac tatttttact atgttccata ctgtatttat cacggattaa
    31621 gtgccctaat tcatgggcag catcaaatcg actacgttct gcagattttt gtgtatttaa
    31681 aaatacaaat ggatgatttt cataccaagt acaaaaggca tcaatgtcct ttgtatctaa
    31741 agataatgaa aatacacgaa cacccttagc ttcaagtagg gtgatcatat tcggaatagg
    31801 ttcattgcca agcccccatt ctaatcttag ttcctgagca gcctcttcag gagaaatatc
    31861 agaaaaatca ggcaatacgg cttgacttag tgtaaattct gtctcgagcc agtcatttaa
    31921 caaaaaagcc gtaatgctat gatttaatgc ttgtttttca agcctcttcg aggtgcgtga
    31981 acgagcacga aaacttactg cctgagattt caactcaggc agtctttcgt cattagtaaa
    32041 gaaatgaact ggaaactcta ataaattggc taattcattt aaatcaggta tttgctcatc
    32101 ttttacatag tttctaacct gtcgagcggt aatacctaat aactcagcta attttgtttg
    32161 cgtacaacca cgtttatcca gcgcaaattc cagtctctca cgattaaatg tctgcatgat
    32221 ttatcattca aattatcctg ctttttgttt tctttcaacc aaaggctcat attcttcaac
    32281 aggttgctta cgttcaagct catcaaattt agttaaatca acatcagcta atataattcg
    32341 ctgcttgtac ccagttattt gatgactaac aaaaccactc ggtaaagata attcaagttg
    32401 cactttatta tacttccagt gaaacagcag aacccaaaac tgcacagtat caggcaaatc
    32461 tagttttgaa ttacgaatag cctcctcaaa tcccttacct ttccttgcgg ttgtcattgg
    32521 catcccgtga tgcctaccaa catctgaagt agcagtagcc acaataatac ttttagttcg
    32581 acatggcgag agacatagaa atgcaccacc cgaccaaggc tcaagcgtcc agccatcttt
    32641 acttaaatat acccttagag caaatgtaat ttctgcttgc cgatacatcc ccaatgtatt
    32701 tctgtcagat aatgctgctt tgtcctgaat attattatgc gcagtaagta caatctcttt
    32761 aagcatctcc tgagataagt acttgctgat ttcacttaaa gcaatatcac tattttgttg
    32821 ctcgactatt tctcctactt caaatgggaa aggttctgat aatgcaaatt ccaccataaa
    32881 aatttcctaa ttttatacgt aatgtttaca caatatatca ggaaatatga aaacgtacaa
    32941 ctatatctat aaagcaatta ataagtagcc tgcccaaccg tgtccttatc tttcggcaca
    33001 cccgacctgc aaatcacgca aaacttggaa tccgtgtgta gggtgtgtgc ggtacatacg
    33061 cacgcagtct ttttaaacca cagcccttcc caactaaacc aaaaggtcgt ctgaacccta
    33121 ttttcagacg accttttgcc actttgtaaa acaaatcttc ccaccatcct ctccccaaac
    33181 atcgcccgaa ccagtaaact tctcatattt caacaactcc ttggaagcaa accatgtctg
    33241 gtatctacct accccgccta ttcccgcccc atatcgccga acgcggcctg ttgtattttc
    33301 agcagggcaa ggttctcgat gtccgaaaaa cttccgccgg gcattatcgg gcggaggtgt
    33361 gcggttcgga aaactattgg gtatggctga agctggatag tgatttgtat attaaagacg
    33421 aaagctgcga ttgcccttat gtctaaaggt gcaaacatac cttaaattac tatattacat
    33481 aggcaaaata caagcctata acgaattgga aacaaaatgc cgtctgaaag cattataaaa
    33541 tctgttcacc ttttcagatg agtaatgtac acccctatac aatttttgct actatacccc
    33601 ataaatccac ggctaaagat atccttatta tgtcctatga ttgatcgaaa cgacttgtaa
    33661 tcggcttagc atcaagtgcc ctattcgagt tatccgaatc ggataatata tttagaatgg
    33721 aaggggcaga aacctatagg caatatcaga gagaaaaaca aaaccatccc ctaaaaaagg
    33781 cgttgtcttt ccatttatta aaaaacttct gtcaatcaat gaaataaacc caaacgaccc
    33841 aacgattggg tttattcttt tatccagaaa caatccagat acagattacg agtcataact
    33901 ataggcttaa tattacacga ttctcattcc atcaaggcgg aaaaccgcac aaatactgaa
    33961 acactatcga tcgatttgta aacaagccta cttaagtaac ttgcagtcct tatcatttcc
    34021 tttaaaataa tccagcccgt cactacacga actggcggac ttcttgcaaa taaaggttac
    34081 tagattttca ttcatcttaa taataaaagg atttttatct ttatctatgg ctaccgcctt
    34141 caacatgaat ttactgtcta aagccccgcg cgcgattcca ttcaaacgga tacaaaagcc
    34201 ttctgcctct ttaatcggca aacttggcca cttggtagat gtttgtttaa acctcccatt
    34261 ctgcagataa aacttttcca taaaatgtgc attttctaac aaggctgccc gcactgcatt
    34321 tatctttgct ttctcaacat aattgcgata gctcggataa acaattaaag caagtacaga
    34381 caatatcaag accactgata ttaattcaac cagcgtaaac ccccgattat cagtcattac
    34441 tttacttcca ataagaacag attattcaac atatttcttt gaacagactt actatcccat
    34501 tcaacagtat gcatatttcc cactctattt tttatc
//
LOCUS       DAWFIE010000013        33207 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWFIE010000013 DAWFIE010000000
VERSION     DAWFIE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33207
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.013"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(304..1563)
                     /gene="rho"
                     /locus_tag="WGQ71_000577"
     CDS             complement(304..1563)
                     /gene="rho"
                     /locus_tag="WGQ71_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ0706640.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDSINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTSNYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(1783..4167)
                     /gene="ppsA"
                     /locus_tag="WGQ71_000578"
     CDS             complement(1783..4167)
                     /gene="ppsA"
                     /locus_tag="WGQ71_000578"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ0706641.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYSGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4579..5400
                     /locus_tag="WGQ71_000579"
     CDS             4579..5400
                     /locus_tag="WGQ71_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ0706642.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(5640..6302)
                     /locus_tag="WGQ71_000580"
     CDS             complement(5640..6302)
                     /locus_tag="WGQ71_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0706643.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTLLARHGLPPKSMDEIRNQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLAPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(6341..7168)
                     /locus_tag="WGQ71_000581"
     CDS             complement(6341..7168)
                     /locus_tag="WGQ71_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ0706644.1"
                     /translation="MKPDIYALLERALLSGNPDEKGRLADEAFAAVQNADGAETNAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAITHIEFNAVNLTLDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPIALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
     gene            7446..8069
                     /gene="lolA"
                     /locus_tag="WGQ71_000582"
     CDS             7446..8069
                     /gene="lolA"
                     /locus_tag="WGQ71_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ0706645.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            8342..9481
                     /locus_tag="WGQ71_000583"
     CDS             8342..9481
                     /locus_tag="WGQ71_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0706646.1"
                     /translation="MKKTLVAAAILSLALTACGGGNDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTQQVKKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARDLM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            9613..10669
                     /locus_tag="WGQ71_000584"
                     /pseudo
     CDS             9613..10669
                     /locus_tag="WGQ71_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            10710..11240
                     /locus_tag="WGQ71_000585"
     CDS             10710..11240
                     /locus_tag="WGQ71_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ0706647.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDDVMIGAGSLVPPRKRLAGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(11251..11390)
                     /locus_tag="WGQ71_000586"
                     /pseudo
     CDS             complement(11251..11390)
                     /locus_tag="WGQ71_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
     gene            complement(11409..13004)
                     /locus_tag="WGQ71_000587"
     CDS             complement(11409..13004)
                     /locus_tag="WGQ71_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ0706648.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMEIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIENPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIEWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLTFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(13111..13506)
                     /gene="hisI"
                     /locus_tag="WGQ71_000588"
     CDS             complement(13111..13506)
                     /gene="hisI"
                     /locus_tag="WGQ71_000588"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ0706649.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(13537..14304)
                     /gene="hisF"
                     /locus_tag="WGQ71_000589"
     CDS             complement(13537..14304)
                     /gene="hisF"
                     /locus_tag="WGQ71_000589"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ0706650.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGNPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAANPENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(14317..15054)
                     /gene="hisA"
                     /locus_tag="WGQ71_000590"
     CDS             complement(14317..15054)
                     /gene="hisA"
                     /locus_tag="WGQ71_000590"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ0706651.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVARDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(15087..15725)
                     /gene="hisH"
                     /locus_tag="WGQ71_000591"
     CDS             complement(15087..15725)
                     /gene="hisH"
                     /locus_tag="WGQ71_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ0706652.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALTRDGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFARDLRDRQGCRLKVPHMGWNTVRQTQNHPLFQGIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(15856..17358)
                     /gene="pta"
                     /locus_tag="WGQ71_000592"
     CDS             complement(15856..17358)
                     /gene="pta"
                     /locus_tag="WGQ71_000592"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ0706653.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSQVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(17543..18601)
                     /gene="fbpC"
                     /locus_tag="WGQ71_000593"
     CDS             complement(17543..18601)
                     /gene="fbpC"
                     /locus_tag="WGQ71_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ0706654.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(18622..20139)
                     /gene="fbpB"
                     /locus_tag="WGQ71_000594"
     CDS             complement(18622..20139)
                     /gene="fbpB"
                     /locus_tag="WGQ71_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ0706655.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPPVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(20207..21202)
                     /gene="fbpA"
                     /locus_tag="WGQ71_000595"
     CDS             complement(20207..21202)
                     /gene="fbpA"
                     /locus_tag="WGQ71_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ0706656.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(21453..21922)
                     /locus_tag="WGQ71_000596"
                     /pseudo
     CDS             complement(21453..21922)
                     /locus_tag="WGQ71_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(21977..23353)
                     /gene="argH"
                     /locus_tag="WGQ71_000597"
     CDS             complement(21977..23353)
                     /gene="argH"
                     /locus_tag="WGQ71_000597"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ0706657.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQNLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAVEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREVLA"
     gene            complement(23372..24241)
                     /gene="galU"
                     /locus_tag="WGQ71_000598"
     CDS             complement(23372..24241)
                     /gene="galU"
                     /locus_tag="WGQ71_000598"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ0706658.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYERSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(24269..24868)
                     /gene="rdgB"
                     /locus_tag="WGQ71_000599"
     CDS             complement(24269..24868)
                     /gene="rdgB"
                     /locus_tag="WGQ71_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ0706659.1"
                     /translation="MSEKLEKIVLASGNVGKLEEFANLFKPYGITVLPQSAFGIPECS
                     EPYSTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGDHPKSDTA
                     NNLKLAAELVGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWHDTPQGQNGFGYD
                     PYFYLPEHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(24861..25094)
                     /locus_tag="WGQ71_000600"
     CDS             complement(24861..25094)
                     /locus_tag="WGQ71_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706660.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAQSAAKENRYV"
     gene            complement(25202..25735)
                     /locus_tag="WGQ71_000601"
     CDS             complement(25202..25735)
                     /locus_tag="WGQ71_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ0706661.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(25837..26295)
                     /gene="nudB"
                     /locus_tag="WGQ71_000602"
     CDS             complement(25837..26295)
                     /gene="nudB"
                     /locus_tag="WGQ71_000602"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ0706662.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            26506..26582
                     /locus_tag="WGQ71_000603"
     tRNA            26506..26582
                     /locus_tag="WGQ71_000603"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26540..26542,aa:Pro,seq:ggg)
     gene            26740..28566
                     /gene="mafB"
                     /locus_tag="WGQ71_000604"
     CDS             26740..28566
                     /gene="mafB"
                     /locus_tag="WGQ71_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ0706663.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSYQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            28569..29033
                     /locus_tag="WGQ71_000605"
     CDS             28569..29033
                     /locus_tag="WGQ71_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706664.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            29222..29404
                     /locus_tag="WGQ71_000606"
                     /pseudo
     CDS             29222..29404
                     /locus_tag="WGQ71_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
     gene            29416..29703
                     /locus_tag="WGQ71_000607"
     CDS             29416..29703
                     /locus_tag="WGQ71_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEZ0706665.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            30199..31461
                     /locus_tag="WGQ71_000608"
     CDS             30199..31461
                     /locus_tag="WGQ71_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ0706666.1"
                     /translation="MKSKSGVIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEA
                     LKKDKQTARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKE
                     SLYYLNKHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGS
                     CAETFGLGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEY
                     VQFSIDLFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQS
                     SQFKAHAQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGV
                     YQATVEIKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAWEKRKELEGQK
                     WRGTSKSGVEIEGFTEPNRTAYPLYNSK"
     gene            31488..31907
                     /locus_tag="WGQ71_000609"
     CDS             31488..31907
                     /locus_tag="WGQ71_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706667.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            31985..32068
                     /locus_tag="WGQ71_000610"
                     /pseudo
     CDS             31985..32068
                     /locus_tag="WGQ71_000610"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32065..32307
                     /locus_tag="WGQ71_000611"
     CDS             32065..32307
                     /locus_tag="WGQ71_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ0706668.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            32385..32630
                     /locus_tag="WGQ71_000612"
     CDS             32385..32630
                     /locus_tag="WGQ71_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0706669.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            32633..33023
                     /locus_tag="WGQ71_000613"
                     /pseudo
     CDS             32633..33023
                     /locus_tag="WGQ71_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33146..>33207
                     /locus_tag="WGQ71_000614"
     CDS             33146..>33207
                     /locus_tag="WGQ71_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0706670.1"
                     /translation="MKTLLLLIPLVLTACGTLTG"
BASE COUNT         7848 a         7783 c         9373 g         8203 t
ORIGIN      
        1 aggcaactct aacttaattt gaatttccct agttatctag gacagcccca aatttataca
       61 aaaatgagcg cggttcggcg caaccttgaa tcaagttccc gcatcggttt tcattgccgg
      121 tacggatgcg ttcaagccgg ctttgcaaag gccgcgcttt cggcaagcgg acacggacac
      181 tgccgacggt tgcgccgtta acggggggag ggaggagctg cgccgaccgt gtgaatgaaa
      241 gtgccgtctg aaacccgatt ttcaggcttc agacggcatt tcgcattaat gcgggtggcg
      301 cgtttatttg ccgcgcatca gttcaaagaa atcatcattg tttttagagg ctttgatttt
      361 cccgattaaa aattcggctg cctcgatttc gtccatcggg tgcaggaact tgcgtaagag
      421 ccacatacgt tgcaactggt cgttcggcac aagcagctct tcgcggcgcg tgccggattt
      481 gttgatgttg atggcgggga agaggcgttt ttccgccata cggcggtcaa ggtgcaattc
      541 catattgccg gtgcctttga attcttcgta aatcacatcg tccatacggc tgccggtttc
      601 aaccaatgcg gtggcgatga tggtcagcga accgccttct tccacgttgc gcgccgcgcc
      661 gaagaaacgt ttggggcggt gcagtgcgtt ggcatcgaca ccgccggtta ggattttgcc
      721 cgaggtaggc acgacggtat tgtaggcgcg ggcaaggcgg gtaatcgaat ccagcaggat
      781 gaccacgtct tttttgtgtt ctaccatacg cttggctttt tcaagcacca tttcggcaac
      841 ttggacgtgg cgttgcgccg gttcgtcaaa ggtggaggaa accacttcgc cgcggacgga
      901 acggctcatt tcggttactt cttcggggcg ttcgtcaatc aagaggacga tgagttcgac
      961 ttcgggatag tttgaggtaa cggcgtgggc aatgttttgc agcatcacgg ttttaccgct
     1021 tttgggcggg gcaaccaaga gggcgcgctg acctttgccg atgggcgaaa tcaggtcgat
     1081 ggctcgtccg gtcaggtttt cttcggactt taagtcgcgt tccagcttca actgttcggt
     1141 cggaaacagc ggggtcaggt tttcaaacag gattttatgg cggcatactt ccgggtggtc
     1201 gccgttgatg gaatccagtc tgactagggc aaaatagcgt tcgttgtctt tggggacgcg
     1261 cacgctgcct tcgatggtgt cgcccgtatg caggttgaag cggcggattt gggtgggcga
     1321 gacatagatg tcgtcggggc ccgcaagata ggacgtgtcc gcgctgcgga ggaagccgaa
     1381 gccgtcgggc aggatttcga gcgtgccgga gcaggtgaaa ccctcgcctt ttttcatcat
     1441 ttggcggacg atggcaaata cgaggtcttg tttgcggaat cggttggcgt tttcgatgcc
     1501 gtgttcttcc gccaattcta agagtttgga aatgtgcagg gtttgtaatt cggagacgtg
     1561 cataataatg atgtattttg aagaggaaaa agacaggcag atgccgtctg aaagaagaag
     1621 ctgaccgttg gcggttgctc ggggaagggg gaattgtagg cagtcggcgc gtgggtgtca
     1681 aatattatcg cggacggggc atcggcagga aatgccgtct gagcggagct gcttggaaaa
     1741 aaataccccc gcgcttttca ggctcggggg tatgggcatt gattatttgt tcaattcatt
     1801 cgccaaatat agccaagttt cgatgacggt atccgggttc agggaaacgc tttcaatgcc
     1861 ttcctcaacc agccatttgg cgaagtccgg atggtcggac gggccttgac cgcagatgcc
     1921 gacatatttg ttctgcttgc ggcaggcgga gatggcaagg tgcagcatca ctttgacggc
     1981 agggttgcgt tcgtcaaacg attcggatac caagccgctg tcgcggtcga gaccgagggt
     2041 cagttgggtc atgtcgttcg agccgatgga gaagccgtcg aagtattgca ggaattgttc
     2101 cgccaatacc gcgttgctcg gcagctcgca catcataatc aggcgcaggc cgtttttgcc
     2161 gcgttccaag ccgttttctt tcagggcttt gacaacggct tcggcttcgc ccaaagtgcg
     2221 gacgaacgga atcatgattt caacgttggt caaccccatt tcatcgcgga cgcgtttcaa
     2281 ggctttgcat tccaaggcga aacagtcttt gaagttgtcg gcgacataac gcgccgcgcc
     2341 ccggaagccc aacatcgggt tttcttcatg cggttcgtat acgttgccgc cgaccaggtt
     2401 ggcgtattcg ttggatttga agtcggacat acggacgatg gttttacgcg gataaaccga
     2461 tgcggccaat gtcgccacgc cttcggcgat tttatcgacg tagaagtcga caggggacgc
     2521 gtaaccggcg atacggcggg taatttccgc ttttaattcg tcgtcttgtt tgtcaaattc
     2581 caacaaggct ttggggtgga taccgatttg gcggttgatg ataaattcca tacgcgccaa
     2641 gccgatgcct tcgctgggca ggttggcgaa gctgaatgcg agttcgggat tgccgacgtt
     2701 catcatgact tttacaggtg ctttaggcat gttgtccaaa gcaacatcgg taatttgtac
     2761 gtccagcagg ccggagtaga tgaagccggt atcgccttcg gcacaggata cggtaacttc
     2821 ttgaccgttt ttcagcaatt cggttgcatt gccgcagccg acaacggcag gaatacccag
     2881 ttcgcgcgcg atgatggcgg cgtggcaggt acgtccgccc ctgttggtca cgatggcgga
     2941 agcgcgtttc atcaccggtt cccaatctgg gtcggtcatg tcggtaacca gtacgtcgcc
     3001 ggcttcgacg gaatccatct cggaagcatc tttaatcagg cgtaccttgc cctgaccgac
     3061 tttctgaccg atggcgcggc cttcgcacaa gacggttttt tcgccgttga tagaaaagcg
     3121 gcgcaggttg cggctgcctt cttcctggga tttgacggtt tcgggacggg cttgcaggat
     3181 gtagagtttg ccgtccaagc cgtcgcgtcc ccattcgatg tccatcgggc ggccgtagtg
     3241 tttttcgatg gtcagtgcgt aatgcgccaa ctcggtgatt tcttcgtcgg taatggagaa
     3301 gcggttgcgg tcttcttcgg ggacatcgac gttggttacc gatttgccgg cttctgcttt
     3361 gtcggtaaaa atcattttga tgtgttttga gcccatggtt ttgcgcagga tggcaggttt
     3421 gcctgctttc agcgtgggtt tgaacacata gaattcgtcg ggattgactg cgccttgtac
     3481 gacgttttcg cccagaccgt aggatgaagt gacaaagacg acttggtcgt aaccggattc
     3541 ggtatcgagg gtgaacatca cgccggacgc gccgctgtcg gaacgcacca tacgctgcac
     3601 gccagcggaa agggcgacga tgtcgtgttc gaagcctttg tggacgcggt aagaaatggc
     3661 gcggtcgttg tacagggagg cgaatacatg gcgcatcgct tctttaacgt tttccaagcc
     3721 gttgatattc aggaaggttt cctgttggcc ggcaaatgat gcgtcgggca ggtcttcggc
     3781 ggttgcggaa gagcgtacgg caacggaaat gtccgcgccg ccggcatcgg caaccatttt
     3841 gttccatgcc gcttcgattt cggcatcgag ctgttcgggg aagggcgtgt ccaaaatcca
     3901 ttggcggatt tctttgccga cgcgtgccag ttcggcaacg tcttcgacat ctaattttgc
     3961 cagtgcggcg gaaatgcgtt cgctcagacc gttgtgtgcg aggaatgcgc ggtaggcatc
     4021 ggctgtggtg gcaaagccgc cggggacgcg aacgcctttt tcggtcagct gactgatcat
     4081 ttcgcccagc gaggcgtttt taccgcccac gcgttcaaca tctgtcatac gcaggttttc
     4141 aaaccaaatt acgtagttgt cggccatttg tgtgtccaat ccaaaatatg ttaaaaaaga
     4201 aacaaatccg cgtgcttatt ttaagcgatt cgttcctctg ctgtcatgtg ttttatccgt
     4261 tttaaaatca tgatgccgtc tgaaaaattg cggtttcggc gtgtgtagcg gtttgaaact
     4321 tgcagccggt atacttcttt ttttgggtat tttctttgta aaacaggtgg tttgaatagg
     4381 ttaatgtttt ttctgtttga tttttttgtt tattttttaa aattttctgc caaaaaatac
     4441 tttatataaa taattttatt tcaaaattat attgtgtctg tttgggtgta atccgaggta
     4501 ggtgtgctgc gagtgctttc cttgtgtctg ccgctgctgt tatgatggga ttttaaacct
     4561 gtgttttaag gatggaagat gagcagtccg cgccatgtgt tttacatttc cgaccgtacc
     4621 ggtctgactg ccgagaatat cggcgaggct ttgctgaacc agtttggcaa tctgtcgttc
     4681 aaacgccata cgcatccgtt tgtcgatacg ccggaaaagg cgcgcgcggt ggtggagaag
     4741 gtcaatcgga gccggcagga aaacggtcag cgtccgattg cgtttgtcag tgtggttgat
     4801 gacgaaatcc gtcggattat caaaggggcg gatgcttttc agattaattt ctttgagact
     4861 tttttgggac tgttggagaa agaactcaat accgaagcca cggcatccga gcaggggcat
     4921 cacagtatcg gtaatacgaa gcgttacgat gcgcgtatgg aggcggtgaa tttctcattg
     4981 aaccacgatg acggggtcag cgataagaac cttcaggaag cggatgtaat cttgatgggt
     5041 gtatcgcgtt cgggcaaaac gccgacctgc ctttacctcg ccctgcaata cggcatccgt
     5101 gcggcaaact atccgctgat tcccgacgat ttggaatcgg ccgatctgcc gcgtatggtc
     5161 aagccttata gggataagct gttcgggttg accatccagc cggaacgttt gcaggccatc
     5221 cgccaagagc gccgcccgaa ttcaacttat gccaaaatcg atacatgccg cagcgaggtg
     5281 gcggacgcgc agagtatgtt cagacggcat gggattccgt ttgcgaatac gacggataaa
     5341 tcagtggaag agcttgccgt acacatcctt caggcgtgca agctcaaacg caggttttga
     5401 cgggttttga ttcggtttga aggtggaact gccgtctgaa atcaggtttc agacggcagt
     5461 tttatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacaaatagt
     5521 acggagccga ttcacttggt gcttcagcac cttagagaat cgttctcttt gagctaaggc
     5581 gaggcaacga cgtactggtt tagatttaat ccactatatg tttgtggggc ggatattttt
     5641 cagggctgta ttttgtccag acattcgagc agatcgagcg gcgtgcggat gtggaaatcc
     5701 gcctgccatg agccggtatc gtcttcggga gcgatgtagc cccattcggc gaggacggtt
     5761 ttcatgccgg cgttgcggcc tgcttggatg tcgcgttcgg catcgccgac gtagagtgtg
     5821 tgttgcgggt cggcgtggat ttgtccgcac gcatacagca tgggtttgat gctgggcttg
     5881 ggttcgccgc aggtgtcgcc gctgacgacg acggcgggtg ggatgatgaa gccgagtttg
     5941 ggggcgagtt tgtcggtgaa gcgcatgggt ttgttggtga tgatgcccca tttgatgccg
     6001 cgtctgtcga gttcggcgat gagttcgttc acgccgtcaa agagctcggt gtcttgggcg
     6061 tagcggcggt cgtattcctc cagatattcg gtgcgccatc gggcatagtc gggatggtcg
     6121 ggggtgatgc ctgcgccgag tttgatcagt cctgccgcgc cgtggctggc ttggttgcgg
     6181 atttcgtcca tgcttttggg cggcagtccg tggcgggcga gcagggtatt gagtgcgccg
     6241 ccgaggtcta gggcggtgtc ggcgagcgtg ccatcgaggt cgaacaatac ggcttgtatc
     6301 atgtgttttc cttttttata aagtgcggga ctgaagggtt tcagacggca tgtttatttt
     6361 gtttcaaacc ctgttcgaaa tcttccaaca tatccaattc gaagcggctg aagcccgctt
     6421 tttcccgcgc ttcgatgttc acatagcccc ggaagataaa catatcgtaa cgggcaatca
     6481 ggctgcggaa cagggcaatc ggttccaaac cgcgttcgcg gcagaggtgt tgataccaat
     6541 ggttgccgat ggcgacatgg cccacttcgt cgcggtaaat gatgtccaat acgccgcagg
     6601 tttccgaatc gccgcgctgc gccaccttcg cgcgtatccc cggcgtaaca tccagcccgc
     6661 gcgcttccaa tacgcgcggt accaacgcca tacgcaacag cgggtcgtag gcggttttgt
     6721 acgccatatc ccacaaatgg ttgtgcgcct cgaaactgcc gtaatcgaag ccgaaagcgc
     6781 gcaatctgtc gcgcatcagg cggaaatggt acacctcttc cttcgccact ttcacccaat
     6841 cgcggacaaa ctgaaacggc agcgtgcgga aacggtatgc cgcgtccaaa gttagattga
     6901 cggcgttgaa ctcgatatgc gtaatcgcgt gcagcatcgc cgcataacct tcggttgtgt
     6961 tcattttgcg cggggtcagt tgcgacggcg cgaccaaaac aggcttgtct ggccgtcccg
     7021 ctcgggggaa gtccgccggc ggcgcgtttg tttccgcccc gtccgcattt tgaacggcgg
     7081 caaacgcctc atccgccagc cgtccttttt catcggggtt gcccgaaagc agggcgcgtt
     7141 ccagcaaagc ataaatatcg ggtttcattt caagtccgcc gtgttcggaa aacaaatatt
     7201 atagcgttta aaaaaaacaa gatgaggcat ataatctccg cgattcggca ttccgcgccc
     7261 aaaccgtcaa atcacgcggc acacaattaa agggcagcgt ggcgcgccta ccttttccga
     7321 gcggtcaaaa aaatcagccc tcggaaaacg cggtttgcaa aatgcaaacc gcccgtaacg
     7381 ccgcccgtat gattgttttg ctgcgccgat actttacgcc acacccatcc caacaaggaa
     7441 aaataatgat gaaaccgcac aacctgttcc aatttctcgc cgtttgctcc ctgaccgtcg
     7501 ccgtcgcttc cgcacaggcg ggcgcggtag acgcgctcaa gcaattcaac aacgatgccg
     7561 acggtatcag cggcagcttc acccaaaccg tccaaagcaa aaagaaaacc caaaccgcgc
     7621 acggcacgtt caaaatcctg cgcccgggcc tcttcaaatg ggaatacact tcgccctaca
     7681 aacagactat tgtcggcgac ggtcaaaccg tttggctcta cgatgttgat ttggcacaag
     7741 tgaccaagtc gtcccaagac caggccatag gcggcagccc cgccgccatc ctgtcgaaca
     7801 aaaccgccct cgaaagcagc tacacgctga aagaggacgg ttcgtccaac ggcatcgatt
     7861 atgtgctggc aacgcccaaa cgcaacaacg ccggctacca atacatccgc atcggcttca
     7921 aaggcggcaa cctcgccgcc atgcagctta aagacagctt cggcaaccaa acctccatca
     7981 gtttcggcgg tttgaatacc aatccccaac tctcgcgcgg cgcgttcaag tttaccccgc
     8041 ccaaaggcgt ggacgtgttg agcaactgat gccgtccgcc ccgatgccgt ctgaaagccg
     8101 ccgaggcttc agacggcatt ttcacgcagg cggaacaatg tcccgcatta ccgcccgttc
     8161 gggcatcgga acggcaaacc cgtgaaaatt aacggttgcg ccccggttgt ttttgccgtt
     8221 taatgcaaac cttgctgcac caagggccaa gaaagccgac cggccgcccc cacagcttcc
     8281 gatgcaggcg gcccggccgt ccctgcaatg ttttttattt ttgaacgaaa ggtcgaaaac
     8341 catgaaaaaa acactggtgg cggcggcaat cctgagcctc gccttgactg cgtgcggcgg
     8401 cggaaacgat accgccgccc aagccccctc cgccaagccc gaagccgaac aatcgggcaa
     8461 actcaatatc tacaactggt cggattatgt cgatcccgaa accgtcgccg cctttgaaaa
     8521 agaaaccggc atcaagacgc gttccgatta ttacgacagc aacgaaacac tggaggcaaa
     8581 agtcctgacc ggcaaatccg gctacgacct gaccgcgccg tccatcgcca acgtcggccg
     8641 gcaaatcaaa gcgggcgcgt atcagaaaat cgacaaggcg caaatccccc attacggcaa
     8701 catcgataaa gatttgctga aaatgatgga agccgtcgat ccgggcaacg aatacgccgt
     8761 cccctatttc tggggcatca ataccttggc aatcaatacc cagcaggtga aaaaagcatt
     8821 gggtacggac aagctgcccg aaaacgaatg ggatttggtg ttcaaacccg aatacaccgc
     8881 caaactcaaa tcctgcggca tcagctattt cgacagcgca atcgaacaga ttcccttggc
     8941 gttgcactat ttgggcaaag accccaacag tgagaatccc gaagacatca aagccgccgt
     9001 cgatatgatg aaagccgtcc ggggcgacgt gaaacgcttc agctcttccg gctatatcga
     9061 cgatatggcg gcgggcaacc tgtgtgccgc catcggttac ggcggcgatt tgaacattgc
     9121 caaaacccgt gccgaagaag ccgcaaacgg cgtggaaatc aaagtattga ccccgaaaac
     9181 cggcgtgggc gtgtgggtgg attcctttat gattccgcgc gacgcgcaaa acgttgccaa
     9241 cgcccaccgc tatatcgact acacgctccg gcccgaggtg gcggcgaaaa acggcagctt
     9301 cgttacctac gcgcccgcca gccgccccgc gcgcgatttg atggatgaaa aatacacctc
     9361 cgacgcatcg attttcccga acaaagaact gatggaaaaa agtttcatcg tatcgcccaa
     9421 atccgcagaa tccgccaaac tgggcgtgaa gctgtggcaa gggctcaaag cgggcaaata
     9481 accggactcc ctgccgtctg aaacctttcg ggcggcagga aacggcgcgt ccgttatcaa
     9541 acagggggcg tttccccgcc cgcctgttat gattgggtta agattaaaat gatttagtaa
     9601 aatgagaaag atatggattt aagtatcgta gttcccattt ataatgtcga gagttatttg
     9661 gaagcgtgtc taaattccat agaacctata ttaagtaatg aaaatgtcga acttatcctt
     9721 gtgaatgacg ggtcaaaaga cggaagtgaa gatatatgtt ataaatatat agataaaaca
     9781 acaaacaaca aacaacaaac aacaaacaac aaacaacaaa caacaaacaa caaacaacaa
     9841 acaacaaaca acaaacaaca ccagataccg aacaccaaac atcaaatata tatatcagga
     9901 taaccaaggg acggcagaag ctaggaatac cggaataaaa aattcaaacg gaaaatatat
     9961 agtctttatt gattcagatg attttattaa ttgtcaggtc ttgctggatt ttcttagtaa
    10021 agatgatatt gatatgccgg atgtggtttt tttaaatgcg gttaaatatg ataagggaag
    10081 tgtttcacat tttggcgaag attatcagcc tgaaaaaata ctcaatcaat ccaaagtcga
    10141 agttttgaaa ggattatgcc gatttagaaa atttccgggt tcggcgtgta ataagattat
    10201 aaaaagggaa ttgattatca aagaaaaact gttttttgaa aaagaggttt atgccgaaga
    10261 tatcgaatgg tcaatgagat tatttaatgc ggcaacaact ttttcttatt tggacggttg
    10321 ttattactat tatcggcagg gaagaaaaga ttctaaaaca ggaactgttt cggaaaaaag
    10381 tataaagtca ttattatata ttttggagaa aaatgcggaa atggaatttg atagggatat
    10441 atcaagttat ctttattctt ttctttccta cgaatatctc gttttgcttt ttatcatgac
    10501 gagtaaaaat atagagtgtg atgctgatat aaaaagaagg gcgtatcatt taaggtttat
    10561 gctgttaaag tccaataaat tgatatataa gctgatattc ccgataatca cattattcgg
    10621 ggtcgatatt acaggcagga ttttaaaagc aatcaggggg aatatttaat aaatccttta
    10681 acactataca tcttaccgaa ggaggaaaaa tgaacgcaat ccgaactttc caaaaccgca
    10741 cgcccgaaat ccacgaaagc tgtatgatag acgaagcgtg cgtcgtcatc ggcgaagtgt
    10801 cgcttgccga agacgtttct gtgtggccgt gcgccgtgtt gcgcggcgat gtgaacagca
    10861 tcaccgtcgg cgcgcgcagc aatatacagg acggcagcgt cctgcacgtt tcccacaaaa
    10921 ccgccgccaa acccgaaggg tcgccgctgg ttatcggcga agacgttacc gtggggcaca
    10981 aagtgatgct gcacggctgc cgaatcggca accgcgtctt ggtcggtatg ggcagcaccg
    11041 ttttggacga tgccgttatt gaagatgacg tgatgatcgg cgcgggcagc ctcgttccgc
    11101 cgcgcaaacg tttggcgggc ggctaccttt atgtcggttc gccggtcaga caagtgcgcg
    11161 tgctgaccga tgaggaaaaa gcctttttga aatattccgc cgcgcattat gtgaagctgt
    11221 cgaaacagta cgggatgtga aatcacatcg gcgttcttgc gtcagcccca aattcatgcg
    11281 gatgggacgc atccgataac ggtatccgat gcgccttgat tttgaccgtc tgcgtttgag
    11341 ttgcaggcaa aaatgccgtc tgaagccctt ttttcgggtt tagacggcat tttattgccg
    11401 attgcttttt aaagtttgac cgaatgctcg cgcgtttcgt ggaacacgat gtccggccag
    11461 cgttcttgcg tgagtcccag attcacgcgg ttaggggcga ggtaggcgag gttgccgccc
    11521 gcgtcgatgg cgaggttgcc cgcgttggct ttttcaaatt ccgccagttt tttcttgtcg
    11581 tcgcacgata cccagcgcgc cgaccagatg gatgcgctgt cgaacacggc ttctacgccg
    11641 tattcgttgg ccagccgcga ggtaacgact tcaaattgca acacgccgac cgcgcccaaa
    11701 atcaaatccg cgccgctcat cggtttgaaa acctgcaccg caccttcttc gccaagctgt
    11761 tgcaaacctt tttgcagttg cttgattttc agcgggtttt tgatgcgaac gctgcggaac
    11821 agttcgggcg cgaagaatgg gatgccggta aacgtcagtt gttcgccttc ggagaagctg
    11881 tcgccgattt ggatgttgcc gtggtttggg ataccgataa tgtcgccggc gtaggcttct
    11941 tcaaccagct cgcggtcgtg ggacatgaag gttaccacgc tggaggcggc gatttcgcgg
    12001 ttgatacgca ggtgtttcat tttcatgccg cgctcgaatt tgccggagca gacgcgcaag
    12061 aaggcaatac ggtcgcggtg tttcgggtcc atattggctt ggattttgaa gataaatccg
    12121 gaaaacttcg gctcgtccgg ctcgaccata cgcacggtcg catcgcgtgg tttcggcgcg
    12181 ggcgcccatt caatcaatga attgaggatt tcctgaatac cgaagttgtt aatcgcagag
    12241 ccgaagaata cgggcgtgag ttcgccggcg aggaattcgt cgagattgaa ctcgttggaa
    12301 gccgcctgca ccaattcgat ttcgtcgcgc aactgttgga tttccaatgg aaagcgttgt
    12361 tccaattcgg gattttctat gcctttgatg atgtcgaact cgtgcggcag gcgttcgccg
    12421 cccgcttcaa agagatagat ttcgtcgttc aggatgtggt atacgccttt gaagtttttg
    12481 cccataccga tcggccaggt aacgggcgcg cagcggattt gcaggatgtt ttccacttcg
    12541 tccaagagtt ccagcgaatc gcgcacttcg cggtcgtatt tgttcataaa cgtaacaatc
    12601 ggcgtatcgc gcaggcggca gacgtttaag agcttgatgg tttgcgcttc cacgcctttt
    12661 gccgcgtcga tgaccattaa tgcgctgtcc acggcggtta aaacgcggta ggtgtcttcg
    12721 gagaagtctt ggtgtcccgg cgtgtccaag aggttgacgg tgtggtcttt gtagtcgaac
    12781 tgcatcacgc ttgatgccac ggaaatgccg cgctgcttct cgatttccat ccagtcggaa
    12841 gtggcgaatt tgccggtttt tttgcctttt accgtacccg cgctttgaat cgcacctgaa
    12901 aacagcaaga gtttttcagt caacgtggtt ttacctgcgt cagggtggga gatgatggca
    12961 aacgtgcggc ggcggcgcac ttggtcgagg atttcttggg acatggtttt ctttgcaaaa
    13021 aggttcaggc cgcttttcag acggcccgga cagtgtttga gacggcgaaa ttgtacaaaa
    13081 aaatgcctga taattcaatg ttggaggcgg tcagtgcgtg ctgccgtaaa tctctttttc
    13141 gtctttcagg acggcatcgg cggtttccca cgcgctgcca cgccagactt tgtaaaagca
    13201 gctttctcgc ccggtgtggc aggcgatgcc gccgttttgg gcgatgagca tcacaatggc
    13261 gtcgccgtcg cagtcgaggc gcagttcgcg gactttttgc gtgtgtcccg actcttcgcc
    13321 cttcatccat tgtttttggc gcgaacggct gtaatagtgg gcaaagccgg tttcgacggt
    13381 tttttgcagg gcttcggcgt tcatccacgc caccattaaa acacgtttgg tttcggcatc
    13441 ttgggcgatg gcgcaaacca aacctttttc gtcaaatttg acggcttcaa gcaggttttt
    13501 atccatattt cctttcagac ggcatggtcg gggcggtcag aggcgcactt cgatgccggc
    13561 ttcgcgcata gcgcgtttgg cttcgcggat ggcgatttcc ccgaaatgga aaatgccggc
    13621 ggcaagtacg gcatcggctt tgccttcggt tatgccttca atcaggtgcc ggacattgcc
    13681 gaccccgccg gaggcgatga cgggaatgtc gacggcttcg gcaacggcgc gggtcagcgg
    13741 caggttgaaa ccctgtttcg taccgtccct gtccataccg gtaagcagga tttcgcccgc
    13801 gccgcgtttt tgcatttcga tcgcccattc caccgcatcc aaaccggtcg gattgcgccc
    13861 gccgtgggta aagatttccc agcgtgtgtt ttcggggttg gcggctttgg catcgacggc
    13921 ggcgacgatg gcttgcgaac cgaaaaatcc ggcggcttcg tcaattaaat cgggacgggt
    13981 aacggcggcg gtgttgatgc tgactttgtc cgcgccggca ttgagcaggc ggcggatgtc
    14041 ggcaacggtg cgtacgccgc cgccgacggt cagggggatg aagacttgtc cggcaacctc
    14101 ttcgatgatg tgcaggatgg tgtcgcggtt gtcggatgag gcggtgatgt cgaggaaggt
    14161 caattcgtcc gcgccttcgc cgttgtagcg tttggcggct tcgacggggt tgcccgcgtc
    14221 gcgcaaaccg atgaagttca cgcctttgac gacgcgcccg tcttttacgt cgagacaggg
    14281 gatgatgcgt tttgccagtg ccataatcgg atgcctttag tcgagggaat ctgccagttg
    14341 ctgcgcttgg gcaaaatcga tgctgccctc gtaaatcgcg cggccggtaa tcgcgcctgc
    14401 tacgccatgt ttttcggcgg cacacagggc gcggatgtcg tccaagccgg tcagtccgcc
    14461 ggaggcgatg acgggaatgc ggacggcttg ggcgagtttg accgtcgcgt cgatgttcac
    14521 gccgctcatc ataccgtcgc gcccgatgtc ggtgtagatg atgctgttga caccgtcgtc
    14581 ttcaaagcgt tttgccaaat caattacatg atgcccggtt acggttgccc agccgtcgat
    14641 ggcggccata ccgtctttgg catccagccc gacgataatc ctgccaggga aggctttgca
    14701 cgcctcgcgc acgaagtcgg ggtttttgac tgccgccgtg ccgataatca cgtcgttcag
    14761 gcccaaatcc aaatattgtc cgatggtttt caaatcgcgt atgccgccgc cgagctgtac
    14821 ggggatgtct cgggcgacgg cggcaaggat gtctttgatg gcgggcaggt tttgcggaac
    14881 accggcaaac gcgccgttca aatctaccag atgcaggcgg cgcgcgccct gtttgaacca
    14941 gtgcagcgcg gtttcggcgg gcgaatcgga aaagacggtc gcctcttcca tcagcccttg
    15001 tttcaggcgg acgcagcgtc cttctttcaa atcgatggcg ggtatcagca gcatagtttt
    15061 tttccttgtg cggggccgtg tccggcttac cagtttaaaa agtttttcaa catcgtcagc
    15121 ccggcatcgt ggcttttttc ggtgtgaaat tgcgtggcga atacgttgtc tttgccgacg
    15181 atgcaggcaa acggggacgg gtagtcgctt tcgcccaata tggtttcggg attttcgggg
    15241 gcgaaatagt agctgtggac gaagtaaaaa cgcgtgtctt gggggatgcc ttgaaaaagc
    15301 gggtggtttt gggtttggcg cacggtgttc cagcccatat gcgggacttt cagacggcat
    15361 ccctgccggt cgcggaggtc gcgggcaaag cgtctgactt tgccgccgaa ccagcccaag
    15421 ccgtcggtgt ttccttcttc actgtggtcg aataaaagtt gcgcgccgac gcagattccg
    15481 aaaaagggtt tgttttttaa agcatctttg actgcctcgt ccaagccgtc gcgcgtcagt
    15541 gccgccatac agtcgggcat cgcgccctga ccgggaaaaa tgactttgtc ggcgcgggac
    15601 acgcggtcgg ggtcgccgct taaaaagatt tcggtatttt ttccggcaag ctgccccgcc
    15661 gtccggacgg atttcaatac ggaatgcagg ttgcccatac cgtaatcgat aatggcgatt
    15721 tgcatggctt cctcctcttt ttttgcaata tggctgcgat tttaacaaac aaatgtgccg
    15781 ggctgataaa aatgccgtct gaaaacggga gtctgtcttc agacggcata gagtttaaac
    15841 ccggaaagcc gtttgtcagc cttccatttg ttttgcctga acggcagtca gggcgatggt
    15901 gaacacgatg tcttccacca gcgcgccgcg cgacaggtcg ttgaccggtt tgcgcaggcc
    15961 ttgcagcagc gggccgacac tcaagacgtt ggcgctgcgt tggacggctt tataggtgca
    16021 gttgccggtg ttcaggtcgg ggaagaccaa aacggttgcc tgtcctgcga cctggctgcc
    16081 cggggctttg gatttgccca cgcccggcac ggttgccgca tcatattgca gcgggccgtc
    16141 gatggcgagg tcggggcgtt tttcccgggc aagtttggtt gcttcgatga cggtatcgac
    16201 atcggggccg ctgccggagt tgacggtgga gtaggaaatc atcgccactt tcgggtcgat
    16261 gccgaaggct tttgcggaat cggcagactg gatggcgatg tcggcaagct gttgcgcggt
    16321 cgggttcgga ttaaccgcgc agtcgccgaa gacgaggact tggttgggca gcagcataaa
    16381 gaatacgctg gacacgaggc ttgcgcccgg tgcggtttta atcagttgca aagcggggcg
    16441 gatggtgttg gcggtggtgt gaaccgcacc ggataccaaa ccgtccacat cattttgcgc
    16501 catcatcatc gtaccgagta ccacggtgtc ttgcagttgc ttgcgcgcgt cttcgggtgt
    16561 caggcctttg gatttgcgca gttcgcacat cggctcgacg tattgttcga ccaatgaggc
    16621 gggatcgatg atttccaaag agtcgggcag gctgatgccg cgttctttgg caacggcttc
    16681 gacttcttcg cgtttggcaa gcaggacgca gcgggcaatg cctttttcgt ggcagatggc
    16741 ggcggcttgg acggtgcggg gttctgcgcc ctcaggcagg acgatgcgtt tgtcggcttg
    16801 gcgggcgaag tcgatcaggt tgtagcggaa ttgcgccggc gacaggcgtt ttgcttcgcg
    16861 gcctgccaat acggatacgt ctttcagcgc gtcgctcgaa ccgaagaagg tcaggccggt
    16921 tttttcggct gccgcttcgg caacggaggc tgccgcgccg tccacgacaa aaccttccaa
    16981 tacgcccggc gcggcggcga agaactgttt ggcaaggttc agccgatttg ccagttcgtc
    17041 ggcatcggcg ttgtcggaac ggacggcgaa gacggctgcc gcgtcaaggg acaatgccag
    17101 ttcgacgttt ttgcctgcga ggtagatttt gtcggcatcg ggcgcgatgc cttcgatgac
    17161 gaggttggcg gcatcgagtg cggcaacttt gccgaccagt gcgtcgaacc agtcgtcgct
    17221 tttgccttgc gcgagcaggg tttcggcggt tgcgtcaacg gcttggaaaa tttgtgcgtc
    17281 cagtgctttt gcaaaggctt gtgcggcggc ggaggcgtcc agtccggcag atacgggtac
    17341 gatgagtact tttgccatga tatatccttt cgtatgctgc ggtgtgcggc atatgtggtt
    17401 ggaaggggcg gcatataggc agaaacggct gcctgcgtgc cgtgcgtgcc gtgtttggct
    17461 tgaggcgcgc aggttgaata tagcaaacaa attctgtttc caacaagata aatatccgca
    17521 ggcttgtgga tgctgccgcc tttcagaggg tatttccggg gaagaacagg gcgggaccgt
    17581 ccaaatggag gacggcggaa atgccgtctg acagggtggg ggcggaaggg aggttgagcg
    17641 tgaggacggt ttgtccgacc cggagactga tttcggtatg ccgcgctttg ggcgtggttt
    17701 tgagaaccac ggcgtgaatg gaggcggtgg gtgcggaatg ggggtgaagg ctgaactgtt
    17761 ccggacggat gagcagtgtg ccgcgtgtgc ctgcgggtgc gccgctttga acgggcaggc
    17821 ggcccaatcc gcaatcggcg gtgccgtcgg cgttgagcgc ggcggggaac acgatgcctt
    17881 cgccgataaa cagggcggca tcaaggtcgg caggttgtcg gtacaattcg tgagggcttg
    17941 cagtttggag gatgcgcccc tgtttcatca cggcaatccg gtcggcgtat tgcagggctt
    18001 cttcgcggtc gtggctgacg aaaacggcag atttgccgtt ggcgcgcagg gcggcaatca
    18061 tgtcttcgcg aatctggcgg cgcaactgtt cgtccagcgc gctgaagggt tcgtccaaca
    18121 aaatcagttc gggatcgggt gcaagggcgc gggcgagggc gacgcgctgt tgctgtccgc
    18181 ccgaaagttc gtgcggatag cgtccggcaa gttcggaaat gccggtcaat tccaacatag
    18241 cttcgatgcg ctgccgttct tgcgccgtct tgcctttccc gttgcccagc ccgtaggcgg
    18301 tgttgcggta aacggtcagg tgggggaaca gcacaccttc ctgtacgaca taacccaaac
    18361 ggcgttcgcg gacggggagg ttggtatttt tcgagaagat ggttctgccg gaaagcgaaa
    18421 tttcgccaaa atcgggttgt tcaaaaccgg caaggcagcg taaaagggtg gttttgccgc
    18481 agccggacgc gccgacgata aagaggattt cgcccgggtc gaggctgagc gaaatgtcgt
    18541 ttaaaaccgg ggtgttttga aaacttttgg acaggtgtcc gatgtgcagg gcggcggtca
    18601 tggcggtact tcctcaagct gttatttgaa ggcgtatttc ttcagcagga atacggggat
    18661 gccggaaaat aataccagca tcagcgcgta aggggtggcg gcggcgtatt gtgcgtctga
    18721 tgtgtattcc caaacggcgg tggagagtgt gtggacatcg tcggtggtca gcagcagggt
    18781 ggcggtcagc tctttcatca gtttgaggaa gacgagtgcg aatgcggcgg taatgccggg
    18841 caggatggac ggcagtacca acgtcctgaa aataaagaag tgtccgcgcc ccaatgttgc
    18901 gccgacctgt tccatccctt ttgggagttg ttccaaggaa gtcctcaggg tggtttgcgc
    18961 catcggcagg taaagcatga aataggcaag gatgacgacg ataaaggttt ggtaaacggc
    19021 aggggtgtag ttgatgctga aataaaccaa ggatagggcg ataaccaaac cggggacggc
    19081 gtgcagtaaa aacggcagcc tgtctatcca aacggttaaa aaattgcgat agcgaaccga
    19141 tgcccaaaca gggggcaagg cacataatat agtcaaaatc gcacctaaag ccgatacgct
    19201 taaggaacgg ataaaggcat caaatacgga tacgagcgcg aatgtgccgg aagtgccgac
    19261 catcatccaa tgtatcaata cgccaaaggg gataataatg cccaaagtca acaagctgct
    19321 taaaaaaaca atcgcgccga tctgaccggg cagtttgagg gttttgacgg gataaggacg
    19381 ggcaacgcct ttgccgctgt ggtaaatctt ggctttgccg cgaaatatgc tttctccaaa
    19441 tacgacgatg ccgcacaccg ccattaaaac agcggaaagc agggcggcgg tattgttgtt
    19501 gtaggacatt tcgtattctt ggaaaatggc ggtggtaaaa gtggggtagt tcaaaatgga
    19561 taccgcgcca aattcgacca gcatatgcag ggcaatcagt aacacgctgc tgccgatggc
    19621 gggtttgagc tgggggagga tggcggaaaa aaaggtttgc aggcggcttt tgcccaagga
    19681 caggctgact tcttcgtaag acaggctgat gcgtttgagt gccgcctcga cgggcaggta
    19741 ggcgagcggg aacgaggaca ggctcataat catcactgtc ccccaaaagc cttcgacacg
    19801 gaaggtcagg ctgatccagg tgaaacagct gacaaatgcg gggatgcaca aaggcagggt
    19861 gattgccgtc tgaaaaaagg ttttgccgaa gaagcggtaa cgttggaaca aaagggcgca
    19921 ggcaatgccc aaaacaatgg aaatcagggt aacgcccgcc atcatcgtca aggtgttgga
    19981 gagcaaatcc cacatacgcg ggcggaacaa cagttcgacg gcgcggttga tgccgacctg
    20041 ccacgaacgc atagcgacat ataaaaaagg cagggtaagc ggtagggcaa tcagtaggat
    20101 gaggccggta agccaaatgg gtattttttt aggagacata gtgtttttta tcggcaaaac
    20161 gggcggacag tataaatgtc cacccgtttg acaatccgaa aacggcttat ttcataccgg
    20221 cttgctcaag cagccgggtg gcgtgttctt tttcggaaac agtggtggcg gacacttggg
    20281 gtgcttccaa cttggcgatg ggttccaaat tgaaggtgga taccacgtgc ggattcaaag
    20341 gatattcggc acggacggcg gtcagggcac gctgtccttc cttgctggcg aggaaggcga
    20401 cgaatttttt cgcctcatcc ttgttttggg aggattttaa cacggctgcg ccggaatagg
    20461 taacgagtgc gccgggatct ctgtggcgga cgaaattcag gcgggtgtgg acattttgta
    20521 cgcctttttc acgcgcgaaa gcgtgccagt agtagttgtt gatgagggcg gcatcgattt
    20581 cgccgttttc aaccgcttga agggcgacgg agtttttagc gtaaggcttg ccgtattctt
    20641 tcagaccttt gagccatttc aatgcggccg cttcgccttt cagtttgacg atggcgacaa
    20701 cctgttccaa gaacgcgccg gaagtggggg cgtaaccgat gcggtttttc catttcggcg
    20761 tggcgtaatt caggacggat ttttccaaat ctttttcaga cagtttgcgg gtgtcgtaaa
    20821 cgacgacgcg cgaacgtccg ctcagtgcca cccagtcttt tttggcggca accggcacgc
    20881 ccttgccgcg tgtttcgttg atggtggagg cgggcagggg ctctaggagg ttggctgcgg
    20941 aaagggtggc gagtgccggg atttgttcgg aatagaatac gtcggcgggg cttcggctgc
    21001 cttcttcttt gatttgaccg gcaagctggt cgcctttggc gctgttgagt ttgactttga
    21061 tgccggtagc ccgggtaaag gcatctgcaa cggcttgtgc cgcttctttg tgttggccgt
    21121 tgtacacggt aatgtctgcc agcgcggggg ttgcggcggt cagggctgcg gcaagcagtg
    21181 cgtatcggat agatgttttc atatcgattt tctcctaatg aatgagagtg tacaccttgt
    21241 taaaacataa cggtgtgtag tgtattcctt cttctttata aatgcaaata attatttttt
    21301 aaatttgtta ttatccgatc cggttatggt ttgttctgac ttgtattttt tccgtgcatc
    21361 gcgcccgtaa ggcggaagcg gcgggcaatg cgtggcggaa tgtgggtaaa ggcggcattt
    21421 tgatttgtcg gaatgcttga gaacccctct ctttaaaaca gcgtctgaac agggttgccg
    21481 gaatagtatt gccatcccgg cagatacagt ttgtcggggt cttgccaata ttgttcatcc
    21541 agactgttcg gcagtgaggc ggttttgttg tcgagatgtt ttgttatatc ccacatttct
    21601 ttcaggtttt taccttccga ttggaggtgg cggatttctt ttctcaatgc gtctgccgcc
    21661 cgatggatca gcattgcgct gtgtatcgcg ccgatttttt cagaacggaa caagtctttt
    21721 cggcggcggg aaaaccccaa ttgcagacaa attgcagtat gccgccgttg ccgatgtcgg
    21781 cttccgcccg ccaaatcaaa accagctcct gttcacgtcc gtccatgctt tcgagtttgc
    21841 cgtcatgctg ttcaaaaagt ttgtctaccg cctgccacat catctgttcg aagcggtcgg
    21901 cttgggtatc cgtatcggtc atggtgttct tgtcttttaa aaatgccgtc tgaacggctt
    21961 cagacggcat cgggggttaa gccaacactt cacgccagcg tttgacttgg aaacggactt
    22021 gttcaggcgc ggtgccgccc aagtggttgc gcgcgtttaa gctgccttcg ggtgtcagca
    22081 cgccgtaaac gtcgtcggca atcaaatcgc tgaaaccttg taagacttcg agcggcagtt
    22141 cgctcaaatc gacgcccgct tggtcggcgt ggcgcacggc ttgggcgacg acttcgtggg
    22201 catcgcggaa aggcatgcct tttttgacca gataatccgc caagtcggtg gcggtagcga
    22261 agccctgcat cacggcggcg cgcatattgt cgggtttgac ggttacgccg cgcatcatat
    22321 cggcgtaaat ccgcaacgtg tcgataagcg tgtcggcggt gtcgaacaag ggttctttgt
    22381 cttcctgatt gtctttgttg tacgccaagg gttgggattt catcagggta atcagaccga
    22441 taaggtgtcc gatgacgcgg ccggatttgc cgcgcacgag ttcgggcacg tcgggatttt
    22501 tcttctgcgg catgatggac gaacctgtgc agaaacggtc ggcgatgtcg ataaagccga
    22561 aacgcggact catccacaaa atcaattctt cagacaggcg gctcaggtga accataacca
    22621 gcgaggcggc agccgtgaac tcgacggcaa aatcgcggtc ggatacggca tcgagcgagt
    22681 tttggcagat ttgctcgaaa cccagtaatt cggcggtaat ttcgcgccga atcgggtagg
    22741 tcgtaccggc aagggcggct gcgccgagcg gcatacggtt gacgcggcgg cggcagtccg
    22801 ccatccgttc gttatcgcgg ccgagcattt cgacgtaggc gagcatatgg tgtccgaagc
    22861 tgacgggctg ggcgacttgc aggtgggtaa agccaggcat aacggtttcg gcgttttgtt
    22921 ccgccaaatc cagcaatgcc gtctgaaggt tttgaatcag gttttgaata acggtaatct
    22981 gatcgcgcag ccacaggcgg atgtcggtgg cgacttggtc gttgcggctg cggccggtgt
    23041 gcaggcgttt gcccgcgtcg ccgattttgt cggtcaggcg gcgttcgatg ttcatgtgga
    23101 catcttccaa atcggacgac cattcgattt tgccgctgcg gatttcttcg aggatttccg
    23161 ccatgccccg acggatgtcc gccaaatcgc cttcgtccaa cacgccggtt tctttcaaca
    23221 tttgcgcgtg tgccagcgag ccttgaatgt cccattcggc aagccgtcgg tcgaaaccga
    23281 tggaggcggt gtattgtttg acgagttcgg aaacgggttc gttgaaacgt ccggaccagg
    23341 ttttgtcgtg cataaggatt ccttgatgtg gttattcggt acggtatttt tccaaaaggc
    23401 ggcggaaggg ttcgccggtt tcggggtgtt tcagaccgta ggcgacggtg gcttcgaggt
    23461 agcccagttt gctgccgcag tcgtagcgcg tgccttcaaa ggggtgcgcc aggacaaatt
    23521 cgtgatcgag cagcttggcg atgccgtctg taagctggat ttcgttgccc gcgccgcgcg
    23581 gaagattggt taagaggtcg aaaatgcgcg gggtaaggat gtagcgtccg acgacggcaa
    23641 ggttggaggg cgcgtcttcg ggcttgggtt tttcgacaat gccggtaatg cgttggaact
    23701 gtttgagctg ttcggtttcg acgatgccgt atgagccggt ttgcgacggt tcgacagttt
    23761 ctacgcccaa aatgctgttg ccgctgcgtt cgtacacttc gaccatttgt ttgagcgcgc
    23821 cttttggggc atcgattaaa tcgtcagcca aaatcacggc gaagggttcg tcgccgatgg
    23881 cggcacgggc gcacaagacg gcgtgtccca agcccagtgc ttccgcctga cggatgtaga
    23941 ggcaggtaat gttcggtggc aggatgttgc ggacgtgttc cagaagtttg tctttatggc
    24001 gcatttccaa ctcggtttcg agttcgtatg ccttgtcgaa atggtcttcg atgctgcgtt
    24061 tgttgcgtcc ggtaacaaac accatttccg tgcagccggc ttccacggct tcttctacgg
    24121 cgtattggat cagcggtttg tcgacgatgg gcagcatttc tttcgggctg gccttggtgg
    24181 cgggcaggaa gcgtgttccc atccctgcga cggggaagac ggcttttttt atcggtttca
    24241 ttctttttcc tttgtattgt tttgatgttt aaagggcgag tttgcggatt aaatcggcga
    24301 gtgcctgcgc gcggtggctt tcgcggtttt tgacctccga atccaattcg gcggcggttt
    24361 tgccgtgttc gggcagataa aaatacgggt cataaccgaa accgttttgc ccttgcgggg
    24421 tatcgtgcca ctgtccgtgc catacgcctt cggcaataat cggacgcggg tcgtctttat
    24481 ggcggacaaa aaccaatacg cagacgtagc agcagctttt gtcggctttg ccgacaagtt
    24541 cggcggcaag tttcaggttg ttggcggtat cggatttggg atggtcgccc gcgtaacgtg
    24601 cggaatgtat gcccggtgcg ccgtttaggg cggcggcaca gatgccgctg tcgtcggcga
    24661 gtgcgggcag cccgctgtgt ccggcggcgt gccgggcttt tgccagcgcg ttttcgacaa
    24721 aggtggaata gggttcggag cattcgggta tgccgaatgc ggattgcggc aatacggtga
    24781 tgccgtaagg tttgaatagg ttggcgaact cttcgagctt gccgacatta ccgcttgcca
    24841 aaacgatttt ttccagtttt tcagacatag cggttttcct ttgcggcgga ttgggcggcg
    24901 cgtagggatt tgtgccgcag gtagagggcg agtgcgccga tttgcccgaa cagcgcgccg
    24961 aatgcgaaaa gaaaggaggc gacggcgaag gctttgctgc cgacggtcag cagataagca
    25021 cccaagagga cgagcagcat aaatgccagt gtgaagaggg caacagacaa cagatagatt
    25081 tttcggcggt tcatggcgtt cggtcggaaa cggtatgttc ggattatatc cgattgggac
    25141 ggtattccct agagcttgga aaaatgccgt ctgaagcggc gttcagacgg catggcgggt
    25201 gttatgcctg tttgttccaa cgttcgatgg attctttaat gacttttttc gcttcttccg
    25261 catcgcccca cgattcgact ttggtcgtac ctgctttttt caggtctttg taatggttga
    25321 agtggaactc gatttgtttg atgagctgtt gcggcaaatc ggacaaagtt ttgtaggcgt
    25381 tgccgttgtt gcggtcgtcg gcaggaacgc agacgatttt gtcgtccact tcgccgtcgt
    25441 caacgaattt catcacgccg ataacgcgcg cttccaagaa tacgccggtt gccaaaggtt
    25501 gttcggtaac gagcagcacg tccaattcgt cgccgtcttc gtccaaagtt tggggaatga
    25561 agccgtagtt ggtcggtttg gcgaagatgg cgggttcgac gcggtcgagt tggaatgcgg
    25621 cgagtttgcg gttccactcg attttgtggt tgctgccggc ggggatttcg ttgacgacgt
    25681 tgatgatgcc gccgtccaca tcgccggggg tcaggatttg gttgaagtct gccatttggt
    25741 ttcctttgtt tgggaaagtt tgaagtttga aagtatagca caaacgtccg gctgaaaatg
    25801 cgcccgatgc cgtctgaaag ggtgtacggg cgcgtgttac cgtttgccca aaaacctgcc
    25861 cagttccaga atcgcgcgcc tgttggacgg ggaaaacact ttttccgccg cttcttccaa
    25921 gccgaaccag ccgtaggaga cgtgttcttc gggttgcagg gcgatgggcg tatcacgcgg
    25981 gatttcggca gagaagaggt gttcgcggtt ttcaaacacg ccttttggat agcggtgccg
    26041 ccagtggtgg tagatttcgt aaaccgtgct gtcgtgccag tcttgaagct gcccgtccgc
    26101 cagcaggatg ccggtttctt cccaaacttc gcgccttgcc gtttgggcga cggtttcgcc
    26161 cggttcgagg ctgccggtta ccgactgcca aaatccttcc ggatgcgtgc gttcgatgag
    26221 caggatgccg ccgtccccgc tataaaggac gaccagtgcg gaaacggggt atttgagcgg
    26281 ttttgccatc ggcatctttc ggcgggctgc ggtaatgaag gggcggatta tagcaaacgc
    26341 cgcacgttat ggcgtttatc cttttccgta tccttttttc tgcacggatg ggacgcgccg
    26401 gtgtttgccg gtaaattttc cgttgtgtca aaaagataag ggcggttgtg attttaatgc
    26461 ttgccaaagc gtcgggcgga aactataatc cgaaacttat cgagtcggag tgtggcgcag
    26521 tctggtagcg cacttgcatg gggtgcaagg ggtcgaaggt tcgaatcctt tcactccgac
    26581 caaaaattcc gaaagccgct ttcaaaagcg gcttttttgc cgtccgtatg attatgatgt
    26641 agagtacgcg gcgacagaca ttcaaatgcc gtctgaaaac cgttcagatg gcatctcttt
    26701 atcttagttt cattccgtac catcttaagg aacatcaaat tgggcatttc ccgcaaaata
    26761 tcccttattc tgtccatact ggcagtgtgc ctgccgatgc atgcacacgc ctcagatttg
    26821 gcaaacgatt cttttatccg gcaggttctc gaccgtcagc atttcgaacc cgacgggaaa
    26881 taccacctat tcggcagcag gggggaactt gccgagcgca gcggtcatat cggattggga
    26941 aacatacaaa gctatcagtt gggcaacctg atgattcaac aggcggccat taaaggaaat
    27001 atcggctaca ttgtccgctt ttccgatcac gggcacgaag tccattcccc cttcgacaac
    27061 catgcctcac attccgattc tgatgaagcc ggtagtcccg ttgacggatt cagcctttac
    27121 cgcatccatt gggacggata cgaacaccat cccgccgacg gctacgacgg gccacagggc
    27181 ggcggctatc ccgctcccaa aggcgcgagg gatatataca gctacgacat aaaaggcgtt
    27241 gcccaaaata tccgcctcaa cctgaccgac aaccgcagca ccggacaacg gcttgccgac
    27301 cgtttccaca atgccggtag tatgctgacg caaggagtag gcgacggatt caaacgcgcc
    27361 acccgataca gccccgagct ggacagatcg ggcaatgccg ccgaagcctt caacggcact
    27421 gcagatatcg ttaaaaacat catcggcgcg gcaggagaaa ttgtcggcgc aggcgatgcc
    27481 gtgcagggca taagcgaagg ctcaaacatt gctgtcatgc acggcttggg tctgctttcc
    27541 accgaaaaca agatggcgcg catcaacgat ttggcagata tggcgcaact caaagactat
    27601 gccgcagcag ccatccgcga ttgggcagtc caaaacccca atgccgcaca aggcatagaa
    27661 gccgtcagca atatctttat ggcagccatc cccatcaaag ggattggagc tgttcgggga
    27721 aaatacggct tgggcggcat cacggcacat cctatcaagc ggtcgcagat gggcgcgatc
    27781 gcattgccga aagggaaatc cgccgtcagc gacaattttg ccgatgcggc atacgccaaa
    27841 tacccgtccc cttaccattc ccgaaatatc cgttcaaact tggagcagcg ttacggcaaa
    27901 gaaaacatca cctcctcaac cgtgccgccg tcaaacggca aaaatgtcaa actggcagac
    27961 caacgccacc cgaagacagg cgtaccgttt gacggtaaag ggtttccgaa ttttgagaag
    28021 cacgtgaaat atgatacgaa gctcgatatt caagaattat cggggggcgg tatacctaag
    28081 gctaagcctg tgtttgatgc gaaaccgaga tgggaggttg ataggaagct taataaattg
    28141 acaactcgtg agcaggtgga gaaaaatgtt caggaaataa ggaacggtaa taaaaacagt
    28201 aactttagcc aacatgctca actagagagg gaaattaata aactaaaatc tgccgatgaa
    28261 attaattttg cagatggaat gggaaaattt accgatagca tgaatgacaa ggcttttagt
    28321 aggcttgtga aatcagttaa agagaatggc ttcacaaatc cagttgtgga gtacgttgaa
    28381 ataaatggaa aagcatatat cgtaagagga aataataggg tttttgctgc agaatacctt
    28441 ggcaggatac atgaattaaa atttaaaaaa gttgactttc ctgttcctaa tactagttgg
    28501 aaaaatccta ctgatgtctt gaatgaatca ggtaatgtta agagacctcg ttataggagt
    28561 aaataaaaat ggcaatttgg aattatcggt actaccttat cccatcagct gctatcagaa
    28621 ataaatttaa tgcaaataaa gatatcatac ttgatgagta tcgatctaat ggttttcaga
    28681 attttaatga gaataaaagt tttgaaaatt actttatcga taatgatgtt atattattat
    28741 caataataaa tgaagcaaaa aaacagctta aattgaaaga atcttgggat aaagacgcaa
    28801 tcatgttttg tgataatttt ggtaatagtc ttaccgtttg gccagatgat atagagtgcg
    28861 aacttgattt aagatttgat tatactaaat ttattcagaa aaccattgat tgggcaataa
    28921 aatataattg tctacttgta atagaaaaaa caggaaatgt agtttcccct aatataaata
    28981 atctgatgta tgaaataaaa gcatatttgg aaagcaagcc gtggcccata tgaaacctaa
    29041 actcaacaag taggatgtgt gcggaacgca cgtatgcggt tctcaaggtt tgagctaaga
    29101 ggccgtctga aaacagaaaa actgtttcag acgacctttc ttttaaccag ttgccacagc
    29161 aaccggacaa aagcagccta cctccacatc catataggca atacagggga gatattttgt
    29221 aaattctacg aatattttac ctgctaaaca gggtaggata tggtatgaag cgaacattgg
    29281 cttaataaac actatgtcaa gatcgaatca ggctggtact agattgttgt attccaatta
    29341 tggattgcta tatataacaa ctgatcatta tatctctgca acacggtttg tagcttggaa
    29401 ataggagtat aacttatgca attagagatt atcggtagta aaatttatac ggaacaagat
    29461 tttcataatc aaatttcaaa aatattttct atacaagatt attatgggaa caatcttgat
    29521 gctttatggg atttattaag cacaaatgta gaacgaccga ttactttggt atggaaagat
    29581 gctatgttct caaaaaatca attagaaaat atatttattg aaatcgtaaa tgttctagaa
    29641 agagttaaga aacaagatga ggattatgga ttcgaagaaa aatttaatta tattttagag
    29701 taagtaaacc ctaattacat tacgtacaca ggcttaaaac tccccagggc caattaagca
    29761 agccgtaacc catataaaac ttaaactcaa caagtagcat gtgtgcggaa cgtacgcatg
    29821 cggttcttaa agtttgagct aagaggccgt ctaaaaacag aaaaaccgtt tcagacggtc
    29881 tttgtttaac gccaccgatc cagcgggtta caaagcgcag tcaatgccgc tgcgccttat
    29941 gcctccgaag caataggcag aacatttgga cacggtgaaa acaaaaacga aaccgcccaa
    30001 gccgtcggac atttcctttt aggagcagct attgcccgcg tcaacggtgg taattttgct
    30061 gccggcggct cggcagcagt tgcagctgaa aaggcggcgg aacatcttgc ccaacagtat
    30121 aacgacggta aaaccgcaat cgatccgcaa acaggcgagt tcaatgccaa cctgctgccg
    30181 gaacatatca aagaggaaat caaatcaaag agcggggtga ttgcatcgct gacgggcgcg
    30241 gccgtgggcg gcacgccggt agatgcgcaa accggaggtg cggtcggaca gaatgcggtg
    30301 gaaaacaacc tctatctgac atcggaagcc ttaaagaagg acaagcagac agctcgtaaa
    30361 atttattccg tcataaaaga gcaagtcaag catgaatgca gttccacagg aagaattacc
    30421 gaatgtcgtc aaaatatagg acgcattatc gaatttaccc aagacaaacg ctttgacagt
    30481 aggtttaagg acttaaaaaa agaatcctta tattacctaa ataaacatcc tgatttagta
    30541 gcctcttatt tgaaggctga atacgaaaag ctggataggg aagacaaaag tatcctgcac
    30601 cgctacatct cacccggggc tgaaatcgtt tcgggcagtt tgggggttgt tctttcagga
    30661 gtagccggag gcggatcttg tgccgagact ttcggcttag gctgtgccgc cgctttggtt
    30721 ggtgtaacgt cttcctacga tcatgtcatt actgggacga aaaacttcgg aaaaaaagcc
    30781 agcgagcaac gaccgacgat tgcggttcag gccttgaagc agttggggct gtcggagcag
    30841 gctgcggaat atgttcagtt ctctatagat ttgttcagtg tgggtaaatc ggggggcggt
    30901 atacctaagg ctaagcctgt gtttgatgcg aaaccgagat gggaggttga taggaagctt
    30961 aataaattga caactcgtga gcaggtggag aaaaatgttc aggaaacgag aagaaggagt
    31021 cagagtagtc agtttaaagc ccatgcgcaa cgagaatggg aaaataaaac agggttagat
    31081 tttaatcatt ttataggtgg tgatatcaat aagaaaggca cagtaacagg agggcatagt
    31141 ctaacccgtg gtgatgtacg ggtgatacaa caaacctcgg cacctgataa acatggggtt
    31201 tatcaagcga cagtggaaat taaaaagcct gatggaagtt gggaaatgaa aaaaggaagg
    31261 ggcggaaaac ctatgacaaa tcattctatg ttcccaaaag attgggatga ggctagaatt
    31321 agggctgaag ttacttcggc ttgggagaaa agaaaggaat tagaaggtca aaaatggaga
    31381 ggtacaagta aatctggtgt tgaaatagaa ggatttacgg aacctaatcg aacagcttat
    31441 ccactttata atagtaaatg aagtattgat taaaaaggtg tttttaaatg tataaaataa
    31501 aatgttttta tgatcagttt agttctggtg aattatttaa ctactgccag tttttagata
    31561 attcttctaa tcaaaagata aatacacgag gaacggtgta tcaatatatt atttatgttt
    31621 taacaggaga cctttatcta caaaaagaca tagatgaaaa tttagaattt atccatcaag
    31681 cagaaaataa tcccaatgaa gtttattcag gaggtggaca aggtttttgc tgggatattt
    31741 ctgctgaaaa agtagttttc tatcacaacg aatttgatga agaagacggc tggcctgatt
    31801 taagctgttc gctgcatact tttaaaactg ctttaattgc ttggaatgct tttttgcaat
    31861 tgcctaaaag tattcattcg gtggtggaga ctgtgattga ggaataagca taattagctt
    31921 aatgaataga atcagcgata tagattggac tgcaaatcca cgcttatacg ctgtgccatg
    31981 attaacagga agagagcaac ttggatgggc tagtagggat ggtaagcatt taaatatatc
    32041 aattgatgga aagattacac acaaatgact aatttaaaat tagatttcta ctctgaagtt
    32101 attataaaag attcttgccc taatgatttg ttagaaaatg gtgagactat taaaggaaaa
    32161 aaaggagtgg tattaggtat aagcgaagaa gatggtataa tctatggcta tactatttta
    32221 ctttttgata taaaatattg tatatatata gataagaaat atattatacc gactggaaaa
    32281 aaattctctc gcgatgattt ttattagcta atttaagtaa tttaagcaag ttttttagat
    32341 actgaaactg gtaattatgt ttatgtaagc ataaggagtt cagattgaag gattctacag
    32401 ggcaaatgag gctggcaacc aaggatttgg cggaagccat taaacgagga gaagtacgta
    32461 gttctgcttt tacaacaaag caactaaagg caatcgaaaa aggagaatct aaaataccgg
    32521 attacacttg gcatcatcat caagacactg gaaggatgca attgattaat gaagacttgc
    32581 atcatgatac cggccatatc ggttggagag cgatgagtaa aggaaagtaa ctatgtggaa
    32641 aatcataaaa gaggatagtg atgatttagg atttgcaatt aaatgcttat tctctcaatc
    32701 tattgattta aatgaattca aattatggat tgaacaagta atacgagata tgcccatcga
    32761 ggacatccct ttttatattt ttgacttggc ggatttcgat ggggtaattg ccgatattga
    32821 caatattgta ggttttgttt cgagttacag cctatcaaaa tcgaaaaaaa aatgccttga
    32881 ccggcattgc cttcttaagg gggatagatg tctatgaccc tcccatttca aaagaaaaag
    32941 cattaaaagc cttagagaaa catcctgaaa tttatcagaa gtttcggcat ttctttccgt
    33001 ttgtagaact gcccccgctt taaacagtca aaataccgtc tgaaagccat ttccgccgct
    33061 cagacggcat tttcgcccct tttgtttaca aacccttaaa atccctttac actcaaaatc
    33121 cgttcaacat caaacaaacc ccgctatgaa aaccctgctc ctcctcatcc ccctcgtcct
    33181 caccgcctgc ggcacactga ccggcat
//
LOCUS       DAWFIE010000014        31911 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWFIE010000014 DAWFIE010000000
VERSION     DAWFIE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.014"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(381..1028)
                     /locus_tag="WGQ71_000615"
     CDS             complement(381..1028)
                     /locus_tag="WGQ71_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ0706671.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKENGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(1025..1393)
                     /locus_tag="WGQ71_000616"
     CDS             complement(1025..1393)
                     /locus_tag="WGQ71_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ0706672.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(1403..2074)
                     /locus_tag="WGQ71_000617"
     CDS             complement(1403..2074)
                     /locus_tag="WGQ71_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ0706673.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVAKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(2239..2958)
                     /locus_tag="WGQ71_000618"
     CDS             complement(2239..2958)
                     /locus_tag="WGQ71_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0706674.1"
                     /translation="MATLSNKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLGGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASTESKGRSGEIVYL"
     gene            complement(3042..4355)
                     /locus_tag="WGQ71_000619"
     CDS             complement(3042..4355)
                     /locus_tag="WGQ71_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEZ0706675.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRTLPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLIDAV"
     gene            complement(4434..5306)
                     /gene="hpnD"
                     /locus_tag="WGQ71_000620"
     CDS             complement(4434..5306)
                     /gene="hpnD"
                     /locus_tag="WGQ71_000620"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ0706676.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTILYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGYLIARILGFSDGKTLQYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            5477..7339
                     /gene="hscA"
                     /locus_tag="WGQ71_000621"
     CDS             5477..7339
                     /gene="hscA"
                     /locus_tag="WGQ71_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ0706677.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRITLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            7552..8175
                     /locus_tag="WGQ71_000622"
     CDS             7552..8175
                     /locus_tag="WGQ71_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0706678.1"
                     /translation="MFVNSLYKIGISDVSNFEGRMRHLENNGYANVAGLERILAVKTD
                     NYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKNETAESEFEKSV
                     HERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSKIRLFKEAYFGK
                     SGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPQ"
     gene            8212..8973
                     /locus_tag="WGQ71_000623"
     CDS             8212..8973
                     /locus_tag="WGQ71_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ0706679.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            9028..9369
                     /gene="fdx"
                     /locus_tag="WGQ71_000624"
     CDS             9028..9369
                     /gene="fdx"
                     /locus_tag="WGQ71_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ0706680.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(9656..10243)
                     /locus_tag="WGQ71_000625"
     CDS             complement(9656..10243)
                     /locus_tag="WGQ71_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ0706681.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDPLSEMGEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHYRSRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLSGSGGGFT
                     VTAESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            10456..10653
                     /gene="iscX"
                     /locus_tag="WGQ71_000626"
     CDS             10456..10653
                     /gene="iscX"
                     /locus_tag="WGQ71_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ0706682.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(10721..11122)
                     /locus_tag="WGQ71_000627"
     CDS             complement(10721..11122)
                     /locus_tag="WGQ71_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0706683.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     NQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            11333..12292
                     /locus_tag="WGQ71_000628"
     CDS             11333..12292
                     /locus_tag="WGQ71_000628"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ0706684.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            12360..13670
                     /gene="tilS"
                     /locus_tag="WGQ71_000629"
     CDS             12360..13670
                     /gene="tilS"
                     /locus_tag="WGQ71_000629"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ0706685.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEIIGQDCRWVCEEGSFDTVRWQAFSPRRKTHILRYFLKENGIPMP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPA
                     LRPFWPVVADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF"
     gene            13721..14506
                     /locus_tag="WGQ71_000630"
     CDS             13721..14506
                     /locus_tag="WGQ71_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0706686.1"
                     /translation="MKHISSANNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGRPVGVYIPEAKMPSEEVRKLTAVLPEDGVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVVLGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLASYKGRVFATALREEKQAVLYNEDLREPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            14516..14713
                     /locus_tag="WGQ71_000631"
     CDS             14516..14713
                     /locus_tag="WGQ71_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706687.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLFWMWWKWF"
     gene            14975..15328
                     /locus_tag="WGQ71_000632"
     CDS             14975..15328
                     /locus_tag="WGQ71_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706688.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSAHKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            15340..15645
                     /locus_tag="WGQ71_000633"
     CDS             15340..15645
                     /locus_tag="WGQ71_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706689.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            15707..17083
                     /gene="mpl"
                     /locus_tag="WGQ71_000634"
     CDS             15707..17083
                     /gene="mpl"
                     /locus_tag="WGQ71_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ0706690.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     KAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKNVKRRMEIKGTVNGI
                     TVYDDFAHHPTAIETTIEGLRQRVGNARILAVLEPRSNTMKLGTMKSALPESLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            17290..18342
                     /gene="bioB"
                     /locus_tag="WGQ71_000635"
     CDS             17290..18342
                     /gene="bioB"
                     /locus_tag="WGQ71_000635"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ0706691.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVHQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLDDAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
                     GEVAEVEKASGIKVDY"
     gene            complement(18421..19056)
                     /locus_tag="WGQ71_000636"
     CDS             complement(18421..19056)
                     /locus_tag="WGQ71_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0706692.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGKFIS
                     GTIIPITQADDKIPKIDPEKTVIGRIIYLNRSDFPNGNGLDVSPDGNITRR"
     gene            19379..19639
                     /locus_tag="WGQ71_000637"
     CDS             19379..19639
                     /locus_tag="WGQ71_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0706693.1"
                     /translation="MTKAVRRKNVQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(19702..21561)
                     /gene="ilvD"
                     /locus_tag="WGQ71_000638"
     CDS             complement(19702..21561)
                     /gene="ilvD"
                     /locus_tag="WGQ71_000638"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ0706694.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(21734..23503)
                     /gene="cysI"
                     /locus_tag="WGQ71_000639"
     CDS             complement(21734..23503)
                     /gene="cysI"
                     /locus_tag="WGQ71_000639"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ0706695.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIDELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVE
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDVSKAVAIARA"
     gene            complement(23530..25344)
                     /locus_tag="WGQ71_000640"
     CDS             complement(23530..25344)
                     /locus_tag="WGQ71_000640"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ0706696.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPTAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFAAAANAWTDNIAALLKEEAAKNRATP
                     APQATPPAGLQTASEGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARVGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFAADFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(25341..26627)
                     /locus_tag="WGQ71_000641"
     CDS             complement(25341..26627)
                     /locus_tag="WGQ71_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ0706697.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDVHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ENLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(26666..27589)
                     /gene="cysD"
                     /locus_tag="WGQ71_000642"
     CDS             complement(26666..27589)
                     /gene="cysD"
                     /locus_tag="WGQ71_000642"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ0706698.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFQLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(27627..28367)
                     /locus_tag="WGQ71_000643"
     CDS             complement(27627..28367)
                     /locus_tag="WGQ71_000643"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ0706699.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIVGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(28378..29820)
                     /gene="cobA"
                     /locus_tag="WGQ71_000644"
     CDS             complement(28378..29820)
                     /gene="cobA"
                     /locus_tag="WGQ71_000644"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0706700.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGSVAARKISLLLDAGAQVRVVANQ
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSYGILSLVRRDAERIFVGKRARGGRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLHEKL
                     AWFGENGEGENRVGQAYPALGGLNAGQRAA"
     gene            complement(30270..30926)
                     /locus_tag="WGQ71_000645"
     CDS             complement(30270..30926)
                     /locus_tag="WGQ71_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ0706701.1"
                     /translation="MKNKSKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            30991..31880
                     /locus_tag="WGQ71_000646"
                     /pseudo
     CDS             30991..31880
                     /locus_tag="WGQ71_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
BASE COUNT         6857 a         8351 c         9283 g         7420 t
ORIGIN      
        1 attatctgaa agtccgagat tctagattcc cgcctgagcg ggaatgacga aaagtggcgg
       61 gaatgacggt tagcgttgcc tcgccttagc tcaaagagaa cgattttcta aggtgctgaa
      121 gcaccaagtg aatcggttcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg
      181 atttttgtta atccactata ccatacaacc acgccggaat taagtttaaa tttgaataaa
      241 aggttcgggt tctgcaaaat acagaacccg aaccttgttc ggatattgaa accggccgcc
      301 cgattttggg cggtgcggct tgcaagtatc aagattcgca tatgccgtct gaagctcgga
      361 gaggttcgga cggcatatgc ttatttgggc tgctcttcga cgaatctcgg acctttcaag
      421 atgccgttgt gagaataggg cgacagcagg ttgtatgcgg cggttttaga aacctgataa
      481 ccgcggtcgg tcaggctgtt ggcaatctga ttgaccactg cgctgaccaa agcccccaac
      541 aggccgctgt tgctgttgtt gctgccttcg cggatgctgg ccgaacccga ccacaactct
      601 tttccgttgc gggaatcgac cagccgtgct ttggcggata cggtcgtcac gctgtctaaa
      661 atttgatatg aagtgccgta ttcggtaacc gtaatgtaca aaaccgcatc attgccgaaa
      721 atctgatgca gtttttccgg ccggacggcg tgaatatcgg cggcattggt caagccgttt
      781 tctttgaagg tttcctccac gactgcggcg gggaagacgt aatagccggc ttcggaaagc
      841 ggagcggcgg tcgaagccag tacaccccat gttccgttga catcgggcga ttcgttcagc
      901 ggcggaacca ccaaaattga agccggtttg ctttccttga atgacgtgta gtcgaaatcg
      961 ggcgcttttt gaacttggca ggcagacagc gccaacacgg cggcaagccc taaaatcaaa
     1021 ggtttcatcg cttgcctcct ttaccggttt tcatcaggaa gtccataaat acgcccgatt
     1081 cgggaaacag ccttttctct tcttcaaact ggcggaacgc gccctctttg tctcccgaac
     1141 gggaaagcag cagtcccaga tgggcgtgcg cacccggggc ggcattcatt tttttgttgc
     1201 cggcttccac aaagtatttt tccatctttt cggtctgctt gcccaacgaa gtgtcgtcgt
     1261 ttttcaaacc ttcatagacg gtatcgggat agccgccgta ataatacagg gatttttgcc
     1321 cgttgccgcc gcaggcggtc agagccaaga ccgccgcaca cagcgacaaa cggctcaagg
     1381 ttttcggatt catcatttct ccttaacggt tgggttgcca tgcgccgttg tcaacagcct
     1441 gaaccaggct gttgacggct tcgcggattg ccaagtctaa aactttgccg ttcaaagtcg
     1501 catcgtagcc ggaagtgccg ccgaaaccga tgatttcacg gttggaaagt gcgtattcgc
     1561 ccgcgccctg tgcggaatag acgatttcgg aagtattgac gttgacgata ttcagagcca
     1621 cttttgcata ggcgatttgc gatttgccgc gacccaaaat gccgaagagc tgatgatcgc
     1681 cgacatctct gcgtccgaat tcggttacat cgccggtaac gacataatct gcacctttca
     1741 ggttatgcgc tttgccggaa atgccggatt cctgttttaa tgcgttcaaa ttggtgcggt
     1801 tcagtacgtt gaagcggttg gtctgttgca ggtgcgttac cagaatggtt tttgcctggc
     1861 tgcccaaacg gtcttccccg tcggagaaaa tgcctttttg gaagctggag cggttgtcga
     1921 atgttccgac ggaaatcggg gtacgaacac cgtgatattg cgtattgtag gaggcgactt
     1981 ttgctacctc gagactgcgt gaggattcgg tcgcacagcc ggtcagtgaa acggcagcgg
     2041 cggcaaggac aacggcggtg gaaacggttt tcataaaatt taccctaagg tcaagttaag
     2101 gaaataacgg gttatcatta ttgtccttat gtaaatttaa gtcaaggtgt ttgtctgtgc
     2161 gggacggatg cgcgcggaag gtacggatat ttttcaaacg gaatcgcggc gcgggcggga
     2221 tgcctgcctt ttccggattt agaggtaaac gatttcgccg ctccgccctt tgctttcggt
     2281 acttgcccac cagacaaatg cgggcagcac gtccccgtag cttttgcgtt cgcttttggc
     2341 ttcgcccgga tgggatttga tgcgttgcgg ggaattgatg ggtccgggga cgaggacgtt
     2401 ggcgcgcagg ttgccgaagc gttcccattc gtcggcggcg actttgcaca ggtagttcaa
     2461 cgcggctttg gacgcgccga agccgcccca gtaggctttg ggtgtttcgc cgtggctttc
     2521 gccgacgaag atgacggacg cgtcgggcga ctgcttcagc agcgggaaca gggcgcgggt
     2581 cagccccata ggtgcgacgg tgttgatgcg gtattggttg acccattcgg cgacggtttg
     2641 gaaatccagc ggcgagaggg cgtaaaaata gccggcgcag tggacgatgc cgcccagttt
     2701 gccttgcgtg gcttcggcaa tggtggcggc gaactgattg aattcttttt cttccgcact
     2761 catcaggtcg aaacggatgg cgaagggttc ggggtgtccg gcttcgacaa tcgcgtcata
     2821 gactttttcc agttttttct gatggcgtgc caccagaatc acggttgcgc ctgccgccgc
     2881 ataggctttg gcgacctgtt cgcccagacc ttgcgatgcg ccggtaacta agatggtttt
     2941 gttggacagt gtcgccatac tttttccttt ttggtggtcg gtcgggggta ttttagcgtt
     3001 ttgccgcacc ttgtaaagcg tcccgatgcc ggccggcgtt ttcagacggc atcgatcagg
     3061 ctttgcaggc aggcttccgc cgacgcgaaa cctgattgta cggcggcttc gagcgtggcg
     3121 gggtagtccg ggtggaggta gtcgccggcg gggaagatgc ggtgccggtg caaccacgac
     3181 aagtccggcg gcggggcatc ggctgcggtt gtggcgcgtt tttcggtgat gacgcgcacg
     3241 gcttcgggtt cgcccaaatg cggaaggatg cgtttgaggt cggcgtgggc tttgtccgcc
     3301 cacgcccggt ttgcaaacgc gccgacgcgg tcggaaacgc tgatgacggc ggacacttcg
     3361 ttttcaggca gtccgagcct gccccggcaa agcagccatt gcaccgtgcc gtcggcaagg
     3421 ccggtcagcg gggcgggcag gcggacgggt tcggcgtagc gcagatagac ggtggtgatg
     3481 gcgtggtagc gaaggttttg atatgccgtc tgaacgtgtt cgggcgtgcc ttcgggcagg
     3541 agcgcggcgg cgtggtaggg cgcggtggcg gggacggcgg catcgaaagc ttcgccgttg
     3601 acgagcactt tcccgtccgg gagggtgttc agacggcata cgcgcgtttc gaggcggatg
     3661 tccgcgccga gccgttgaag ctccgccaag gcgggttcgg cgacgattgc gcccaaatcc
     3721 tgcttgggta ggagatagtc gctgccggat tttttcgtca gcacgccgtc ggacaaaacg
     3781 ttgcacaaca cgcgcaggct tgcggtttcc aaaggcgtgt tgagcgcacc ccacaccagc
     3841 ggctgccaaa actgcatcac ggcggcacgc ggcacgttcc gctgtttcag ccattgcgcc
     3901 actgtcgtgt cgggctgtcc gaggcgtgcg gacttctgca aatcggacat atcggcaagc
     3961 agtttggctt tgaatgcgga cggtacgcgc cgggcaagca gcacgccgcc caaaatatgc
     4021 agcggcgcgg gcagggggag ggtgcggaac tgcaaaccgc cgtgcatatg ccagtgcagc
     4081 ggtacgcgca aaaaggcggc acgggggtct gaaccgatgg ttttcatcag gcgcaacacg
     4141 ccccggtatg cgccgagcaa aatgtgctgc ccgttgtcca aaaaaccgaa accgtcggta
     4201 tttccggcca gtgcgcgcgc cctgccgccc gcctgccggc cggcttcaaa cagggtaacg
     4261 tcggcgtgcc gcgccaaggt gacggcggcg gacagtcctg cccagcctgc gccgatgacg
     4321 gcgattttcg ggcgcggatg cggcgtgttc atcatttatt cctccaatgg ttgaaacccc
     4381 gtttttcggg gcggcggggg ttgggttttc ctgaagggcg ggcggtatgc gccttatgcc
     4441 cgttccggcg tgccggggcg cggtttgaat ccgaataacc aggttttcag ggcaatgcgt
     4501 tttttgcgcg gcgaagggag ggcgattttg tatttgagga cgttttgcgc gccgtcccgg
     4561 tcgatttcgt tcaatagctc gtaataaacc gccgccataa ccagtccgac tttttgggct
     4621 tttttatcgg cagccggcag cagcgatacg gcttcgcggt aggtttcacg ggcgcgtttg
     4681 atttggaacg ccatcaattc ggcaaaattg cccgtcgggc tgcattgcaa aatcacgctt
     4741 gcggggacat caaaccgctg catttcctcc atcggcaggt aaatccgtcc cctgcgcgcg
     4801 tcttcgccga catcgcggat gatgttggtc agttgcagcg caagtcccat cttgtcggcg
     4861 tattgcagcg ttttgccgtc tgaaaacccc aaaatccgcg caatcaggta gccgactacg
     4921 cctgcgacgc ggtggcaata cagtttcaat tcctcaaaac tgccgtaacg cgcctgaacc
     4981 aaatccatct gcatcccgtc gattaaggct tccagttcat atttcggcag cttgaaggtt
     5041 tccttaacct gccgcaaggc ctgattgacg gggtgttccg gcatcgcgcc gccgaatacc
     5101 ttgtccaaat cgccgcgcca ccagttcaat gttgcctgtg caacatcggg gttggaacat
     5161 tcgtcaacca catcgtccaa ttcgcggcaa aaggcatata aaatcgttac cgcatcccgt
     5221 ttttcctgag tcaggaaacg gaagcccgat aaaaaactgg agcggctttc ttctgctttt
     5281 tgacggcaat agtcgagtcc tttcacgatt tatattccta atgatgggcg ggaaaggcgg
     5341 attttatcgg catttggcgg tagagggcaa tttcggcggc acgacccaat ccttagcggt
     5401 ttgctcaact atcggcgcga attctgttaa aatgccgact tccttccttt acacaccgca
     5461 ccgacaggca gaatttatgg ctcttttgca gatttcagaa ccgggtatgt ccgccgcccc
     5521 gcaccgacac cgtttggcgg caggcatcga tttgggtacg accaacagct tggtcgccac
     5581 cgtccgcagc ggcagtgccg cctgtctgcc cgatgccgaa gggcgcatta ccctgccttc
     5641 cgtcgtccgc tatctggaaa acggcggcat tgaagtcggc aaaaccgccc tgtccgccca
     5701 aaaaaccgac ccgctgaaca ccgtcagctc cgccaaacgc cttatcgggc ggacgcttgc
     5761 cgatctgcat caaaatacgc attacctgcc ttaccgtttc ggtgacaatc aacgcgttat
     5821 cgaactgcat acgcggcagg gggtgaaaac gcctgtcgaa gtgtcggcgg aaatcctcaa
     5881 aacccttaaa tcgcgcgccg aagaaacctt gggcggcgat ttggtcggcg tggtgattac
     5941 cgtccccgcc tatttcgacg atgcccaacg ccaggccacc aaagatgccg cgcgtctggc
     6001 gggtttgaac gtattgcgcc tgctcaacga acccaccgcc gccgcaatcg cctacgggct
     6061 ggacaacgcc tcggaaggca cgtttgtcgt gtacgactta gggggcggca cattcgacgt
     6121 atccgtattg caactgacca aaggactgtt tgaagtcaaa gccaccggcg gcaacagcgc
     6181 gttgggcggc gacgatttcg accaccgcct gttctgccgc ctgctcgaac aaaacggact
     6241 ctcccaactc aacgaacaag acagccaact cctgctttcg ctcgtccgcg ccgccaaaga
     6301 acaattaacc acgcaaaccg aagcgcgcat tcaggcgacg ctttcagacg gcatggcaat
     6361 cgacacaagc atcagtcgcg ccgagttcca caacctgacg cagcatttgg tgatgaaaac
     6421 gctcgaaccg gtcacacagg cgttgaaaga tgccggtgtc ggtaaaaacg aagtcaaagg
     6481 cgtgattatg gtcggcggtt cgacccgtat gctgcacgtc caacaggcag tcgccacctt
     6541 cttcggacaa accccgctga acaacctcaa tcccgacgaa gtcgtcgcgc tcggcgccgc
     6601 catacaggca aacgtcctcg caggcaacaa aaccgacggc gaatggctgc tgctggacgt
     6661 tacacctttg tcgctcggtt tggaaaccta cggcggcttg gcggaaaaaa tcatcccgcg
     6721 caattccacc atccccaccg cgcgcgcgca ggactttacc accttcaaag acggtcaaac
     6781 cgcgatgacg atacacgtcg tgcaaggcga gcgcgagctc gtttctgact gccgcagcct
     6841 tgccaaattc accctgcgcg gcattccacc gatggcggcg ggtgcggcgc gtatccgcgt
     6901 aaccttccaa atcgatgcgg acgggctgct gtccgtttcc gcccaagaac aaagcaccgg
     6961 cgtacaggcg caaatcgaag tcaaaccttc ctacggcttg gacgacggcg cgattacccg
     7021 aatgcttaaa gacagtatgg acaacgccgc cgaagatatg gcggcacgcg cccgcgccga
     7081 agccgtagtc gaagccgaaa gcctgaccga tgccgtcaac gccgccctcg agttggacag
     7141 cgatttactg gatgccaaag aattgcaaca gatccggcaa ggcatcgccg atttgcaagg
     7201 ccgtctgaaa gacggaaaag ccgaagacat ccgctccgcc gtcgccaaac tcagccgcag
     7261 caccgacaac ttcgccgcca aacgcatgaa ccgcaatatc caacgcgcat tgacaggcca
     7321 gagtgtcgat aatatttgat acttaaacgg tttcagacgg catagatata acctgatgcc
     7381 gtctgaaggc tcgaaaacac ttgaaaaaca tcgatatgga aaagtcaggc attgtctatt
     7441 cgatgaaaac cgtcatcaag ggcgtgtata gtgaattaaa tttaaaccgg tacagcgttg
     7501 gctcgccttg ccgtactatt tgtactgtct gcggctcgcc gccttgtcct gatttttgtt
     7561 aattcactat ataaaatcgg catttcggat gtaagcaatt ttgaaggcag aatgcgccat
     7621 ttggaaaaca acggctatgc gaacgttgcc ggattggaac gcatcctcgc cgtcaaaacc
     7681 gacaattaca aagaaaaaga aaacctgctc catgaaattt tcagcaaaag caggataggc
     7741 gataccgaat tgttcgccgt ggacgaaaac cttgtaaaac gtttgttttt atcgcttcgc
     7801 ggcgaaatcg tgtttccgaa aaacgaaacg gcggaatcgg aatttgaaaa aagcgtccac
     7861 gaacgcaggc aggaagggaa tgccgggtcc ggccgcaaac aactgcttga tttggtacgg
     7921 cgcggacacc gggaataccc ttacgcgctg ccccggcttt tggcgggcgc ggcatcctac
     7981 aagccgaaaa aatcgaaaat ccgccttttt aaagaagcat atttcggcaa aagcggcacg
     8041 aggctgaccg acgaaattgc agacggcatc catatttaca cctgtttttc gcgggcggat
     8101 ttggaaaaag cctattccga atatttggaa cttttcaaat ccgaatcgga tgccgaaggc
     8161 agaaagccgc agtaaggtgc aaacagatac cgtacacgtt gaggagcaga tatgatgggc
     8221 gattccgtca tttattatgt agaacaggca gacaaaccgg taaaccgtgc cggcgaacgc
     8281 gcccgcaaaa cattcaaata tttttggcgc gagctttttt gggaacgccg ccgcatcatt
     8341 cccgccttag attttgccat ggtcaaagcc ccttttttcc aagacggcga agacggcgaa
     8401 atttgcgaac atatgtggat cgacgatatt tattttgacg gtctttacat ttatggcgtg
     8461 ctgaacaatg aacccggcga actgaccaat gtcgaacaag gcgaaagcgt ttgcgttccg
     8521 gttgacgaca tcagcgactg gatgttcgtg tgcaacggca ttccctacgg cggctttacc
     8581 atacaggcaa tgcgtggaca gatgacggaa gaggagcgta ccgaacacga tgccgcatgg
     8641 ggaatcgatt tcggcgatcc cgggcaggta ttgctggtgt atgaagaaaa agaacatccc
     8701 gaaaatctgg aagagcatcc gatgtgccgg aactgtattg acgattttcg gcaacagttg
     8761 tcccaaaatc cggattttct gcatgagcag gacgaagacg gctatacgcc gcttcatcat
     8821 gaagccatcg caggaaatgc acttatggtt caagccatgc ttgaatacgg cgcaaatcct
     8881 gcctcaaaga catcggaagg ctataccgcc ctcgattttg cccgcctggc gggctggcaa
     8941 aatgttgccg acctgctcga accgcgacat taggcggaca gttttccgaa aacgaacaca
     9001 aacacttttt acagaaagac aataaaaatg cccaaaatca ccgtacttcc acacacgaca
     9061 ttatgccccg aaggcgcggt tatcgaagac gcgcccgaag gcaaaaccgt ccttgacgtg
     9121 ctgctcgacc atgatatcga agtcgatcac gcctgcgaaa aatcctgcgc ctgcaccacc
     9181 tgccacgtca tcatccgcaa aggtttcgac agtttggaag aaccgaccga attggaagaa
     9241 gacctgctcg accaggcttg gggtttggaa gccgattcgc gcctgagttg tcaggcggtt
     9301 gtcgccggcg aagatttgat tgtggaaatc cccaaataca ccatcaacca cgcgcgcgaa
     9361 gaacactgaa aacaggccgt ctgaagccgg cacgcttcag acggcattgt tgcgcggata
     9421 aggcgcaatc gcccgaaaac aggcgttcgt acaggcggaa ctttcgattc tatagcgaat
     9481 taacaaaaat caggacaagg cggcgagccg cagacagtac aaatagtacg gcaaggcgag
     9541 gcaacgctgt accggtttaa atttaattca ctatatattg atttttatcg gttttctgac
     9601 ggaacggatt ggtgcggcat ccgaggcgga ttactcgggc gcgatgcacg ggtatttatc
     9661 ggttttgcag ccggaaaaac cgccggcgtg ttactttcag ggcggcggtc gggcggtatg
     9721 ccggatagcg ttccactttc gcttctatat tgattttgat tggttttctg acggaatgac
     9781 ccgatgcggc atccgggacg gcttgtgttt tttcctgccc gcctgccgga ttttcccatc
     9841 cttgcgtgaa accgaaagag acggcggcgc ggcggacaag ctcgagatag cgtccttcaa
     9901 gctccgggca ggcggcggac acgctttctg ccgtaaccgt gaagccgccg cccgacccgg
     9961 aaaggcgttc cgcaatctgc gtgtagatgc gccaacgttg ggcgcgcaac attgccgcat
    10021 cgcaaccggc agccgcttta tccaaggcaa tcaggccgcg cctgctccgg tagtgaaggc
    10081 aaaccgttac tctggagagg atatgctgcc cgtccaatat ttgatacagt ttgcgtatca
    10141 gcaaaatcag gcggtcaaac tcccccatct ccgacagggg gtcgggatgg tcggaagcgg
    10201 tataaaccag tttggacaat tttgcggcaa acatattgtt catcaatctt ccttgtcggt
    10261 tgaaaccccg ctcttcgggg cggtagaatc agatttgttt gggagggaca actcctccca
    10321 gatcaggacg acacataggc tggtgcttga tgtgttgtcc ggcgagttga aacattcagc
    10381 catcctcaag gtgcggcagt tttgccgaaa catattctac acggcttcaa tgccggacga
    10441 taaaaggaaa ttcatatgaa atggaccgac acccggcgta tcgccgaaga actctatgac
    10501 ctgcacggcg aaaccatcga tcccagaacc gtgcgcttta cccaactgcg cgacctgatt
    10561 atggcattgc ccgaatttga tgacgatccc gcccgttgcg gcgaacgtat tctcgaagcc
    10621 gtgcagcagg catggataga cgaggcagaa taagtttcgg gaatgccgtc tgaaatacgg
    10681 cggtacgcgg ttcgtgcttc tgtttgcagt gggaacggtt ttaccagtct ccttttttca
    10741 gcctatccag ttggcggcgg tcgcgcttgg tcggtctgcc gtcgggatgg gcggaagtga
    10801 tgcggctgaa ttggttgagc tgtttgcgct cttccctcaa tgctgccgtt tttgcgtctt
    10861 cttcatacag aagccgcgcc tcggatgccg ggcggcgttg gtggttcaaa cctttaacct
    10921 tgattttata gggaagggaa ttgagcgtca ggtcgataat atcgccgatg tctatggttt
    10981 tactgttttt gaccttcgag ccgtttactt gaaccctacc cagttcgatg tgcttttgcg
    11041 caagggaacg ggtcttgaaa aaacgtgccg cccaaagcca tttgtccagc cgcatggcgg
    11101 aagaatcgtg cttgtctttc atacgatttt gtttgaaata attgaatttg tttcgagttt
    11161 agcataagat acgccgccta taactagtat atatgcacta atccactgtt ttccatgctg
    11221 tccgaacaca aaaagagggt gtggaaaagc cgttttggac aataaattaa ctgcggaata
    11281 tgcacaaata gcgtatgata gcggcagaat ctgttgatga gagcttcatt ctatgaaacc
    11341 tgtttttttg gattttgaac aacccatagc cgaactgacc aacaaaatcg atgagctgcg
    11401 tttcgtccaa gacgagtctg ccgtcgatat ttcggacgaa atacaccgtt tgcagaaaaa
    11461 aagcaacgac ctgaccaaat cgattttcag caaacttacg cccgcccagg tttcacaggt
    11521 ttcccggcat ccgcagcgtc cctatacttt ggattacatt gatgcgctgt ttactgattt
    11581 tgaagagctg cacggcgacc gccattttgc cgacgatcat gcgattgtcg gcggattggc
    11641 gcgtttcaac ggacaaagcg tgatggtcgt cgggcatcag aaagggcgcg acaccaaaga
    11701 aaaaatccgc cgcaacttcg gtatgccgcg ccccgagggc taccgcaaag ccctgcgcct
    11761 gatgaaaacg gctgagaaat tcggcttgcc cgtcatgacc tttatcgata cgccgggcgc
    11821 gtatcccggc atcggcgcgg aagaacgcgg gcagtcggaa gccatcggca aaaacctgta
    11881 cgaactgacg cgcctgcacg tccccgtttt gtgtaccgtc atcggcgaag ggggctcggg
    11941 cggtgcgttg gcggtcgccg taggcgatta cgtcaatatg ctgcaatact cgacctattc
    12001 tgttatctcc cccgaaggct gcgcttcgat tttgtggaaa accgccgaaa aggcggcgga
    12061 tgcggctcag gctttgggca ttactgctga ccgcctgcaa aagctggact tggtcgatac
    12121 cgtcatcaaa gaaccattgg gcggcgcgca tcgggatttt gggcaaacca tgaaaaacgt
    12181 gaaagccgtt ttggaaaaac aactgcacga agcgcaaagc atcccgcttg ccgatttgct
    12241 ttcgcgccgt ttcgaccgca ttatggctta cggcaaattt tcggaacaat aattcaggta
    12301 gaacaagcag caagcagttt gtctgaaact gcttgctttt tctttatcgg gacggaaccg
    12361 tgctgacttt agatgcgttt gagcaatgct tgaaggattg ttttcctcaa ggtctgaatg
    12421 gaaaaaaaac agcggtggca ttaagcggcg gcttggattc cgtcgttttg ctgcatctgc
    12481 ttgtccgcgc cggaaaaaag ggcggtttta ttccggatgc attgcatatc catcacggct
    12541 tgagtccccg tgccgacgat tgggcagatt tctgccaaaa ctattgcgat atgctcgggg
    12601 tggggctgga aacggttaag gtctgcgtgg aaaaaaacgg tttgggcatc gaggcggcgg
    12661 cgcggcaaaa gcgttatgcc gcgtttgccg aaaaaggctt tgacgttttg gcgttggcgc
    12721 accacaggga cgatcaaatc gaaaccttta tgctggcagt cgctcgcggc ggcggtttgc
    12781 gcgctttggc ggctatgccc accgtccgcc cttttgggga aaaaggcatc atctggcggc
    12841 ctttgctgcc tttttcgcgc caagacatat gggattatgc ccaaaaacac ggtttgccga
    12901 atatcgagga cgaaagcaat agcgatacgg cttatttgcg aaaccgcttc cggcaccgta
    12961 ttttgcccga actttcggcg cagattcccc atttcgggcg gcatgtactg aacaatgtcc
    13021 gcgctttgca ggaagatttg gctttgttgg acgaaatcat cggtcaggac tgccgttggg
    13081 tctgtgagga aggcagtttc gatacggtgc gttggcaggc gttttcccca cgccggaaaa
    13141 cccatatttt gcggtatttt ttgaaggaaa acggcatccc catgccgaat cagaatgccc
    13201 ttgccgacat tgcccgggtt ttgacggagg caaaaaccgg acgttggaac ttgcaaggct
    13261 ttgaattgca tcattatgca ggcaggctgt ttgtgttccg actggaaaaa acggataaac
    13321 tgcggttttt gaaagacagg cagataagcg gaaatttaag ggaaatattg gcggggcagg
    13381 gatttgtttt aaaacgtcat ccgttcggac ttcccgggca tcttttggag caggacggaa
    13441 tcttgcggac ggttgcggca tcggacaggc tggctgtcgg cggtatccat aaggatgtga
    13501 agaaaatcct tcaggaaaag cgggttttgc ccgcgctgcg cccgttttgg ccggtcgtcg
    13561 ccgacaagga caaccgtccg ctggcgttgg caaattacgg tacggatttt caaatctcgg
    13621 tttcagacgg cattttgccc gtccatccgg attttcccgt tttattttga tggaatcgga
    13681 atcgccgtta cctattatat attcttgaaa aggcggtaaa gtgaagcata tcagttcggc
    13741 aaacaatgaa cacatcagac acctgcaccg cctgttgtct caagggaagt tcagacggca
    13801 atacgcccaa accgttttgg agggcgtgca cctgcttcag gttttcctgc aatccggcgg
    13861 aaggccagtc ggggtatata ttcccgaagc gaaaatgccg tctgaagaag tccgtaaatt
    13921 gacggcggtt ttgccggaag acggggtttt ttccgtttca gacggcatat tgaagaaaat
    13981 cagcagcctg acttgcgcgg atgatgtgct tgcgctgatt gatattccgg atgcgggtgc
    14041 tttgccggcc ggcggcgact gtgtggtttt ggacggcgtg caagatccgg gcaatgtcgg
    14101 cacggtgttg cggagcgcgg cggcggcagg aatcggcgcg gtcgttttgg gcaaaggttg
    14161 tgcggacgcg tggtcgccca aagtgctgcg agccggaatg ggcgcgcatt tcttgtcgga
    14221 gatttatccg caggcggatt tggaaatatg gttggcgagc tataaaggcc gtgtgtttgc
    14281 caccgcgttg cgtgaggaaa aacaggcggt tctgtataac gaagatttgc gcgaaccgac
    14341 agcctgggtg tttggcaacg aaggcgcggg ggtcggtaaa gcggttttgg acagggcgga
    14401 caagtgtgtc aggattccga tgcacgatgc aaccgagtct ttaaatgtcg cgatggcggc
    14461 gacaatctgc ctgtttgaac agatgcggca gcggtcggcg tattgaggaa aagaaatgcc
    14521 gtctgaaaaa atctattacg gcgtattgat tttcttatgt atcgcttcta tgctgctgtc
    14581 gccgtttttt tatgcgggtg ctttgaagcc caagaaggcg gcattgcgga aggacgggca
    14641 gtggaaactc atcttattgt ccaataccgt ggcggcggcg gttttgtttt ggatgtggtg
    14701 gaaatggttt tgacagatat tgctcaaaat tgggttaatg gaatccgaac aaataaataa
    14761 tttggtaaaa aatttgttaa atcaatggat taaagttttt cgggaaacaa aacagctcta
    14821 agcaaatagg gcgtttgtcg gtaaatacgg aagagttgcg gcattatcgg gcatctttaa
    14881 caagtagtgc cggcttggca ggcaattggt ttttatgggc agcttgcaaa atcgcggata
    14941 taaaattgcg aatcggttaa agtgtgggga cgctatgaaa aattgcgaat ttttttatga
    15001 cccgacaagg gcaatctatg atagcggtgc agattattta accagagaaa aacatagatt
    15061 agtcgtaatt gcaaatagtg cttgggggct attgcttaat ttatcttgtt attatgacga
    15121 ggttttggaa aagcggaaaa taccgttcgg caaacaggaa attgatgacg atatggacaa
    15181 agtgtccgcc cataagcgga agtttaaaga tatttctgaa atcaaagtag gggatggttg
    15241 ggaatacccg ttcaattatg agcagggaat gaaagaatta gatgaagtgc tattgaaata
    15301 cattcccttt tttgaagaag aacgataaag gaggttgata tgcgcgtatc taaaataatt
    15361 ggaagtatgt tgcttgttac agcggttcag accgtatttt cggcaaatgt ttacgcgtgc
    15421 cgccataatg gtaaaaccag ttacagccaa actccgggaa aacattgtac caacgcgggt
    15481 ttggggcggg atcgggtgta cagttcggtt agaccggcag taaaagacag ggcggaagac
    15541 gcaggggtcg gcgattattc ggacacggtg agggacgaac acgtccaaaa tccgagagag
    15601 aatacgcata aggatgcttc gaataccggt gcaaagacgc attgattgaa accgaatcag
    15661 ccatgacggc gttgaataat agaatttgaa cgattaggga actctgatga aacacatcca
    15721 cattatcggt atcggcggca cgtttatggg tgggattgcc gccattgcca aagaagcagg
    15781 gtttgaagtc agcggttgcg atgcgaagat gtatccgccg atgagcaccc agctcgaagc
    15841 cttgggcata ggcgtacacg aaggcttcga cgcggcgcag ttggaggaat ttaaagccga
    15901 cgtttacgtc atcggcaatg tcgccaagcg cgggatggat gtggttgagg cgattttgaa
    15961 ccgcgggctg ccttatattt ccggtccgca atggctggct gaaaacgtgc tgcaccatca
    16021 ttgggtactc ggtgtggcgg ggacgcacgg taaaacgacc accgcgtcca tgctcgcatg
    16081 ggttttggaa tatgccggac tcgcaccggg cttccttatc ggcggcgtac cggaaaactt
    16141 cagcgtttcc gcccgcctgc cgcaaacgcc gcgccaagac ccgaacagcc aatcgccgtt
    16201 tttcgtcatt gaagccgacg aatacgacac cgcgtttttc gacaaacgct ccaaattcgt
    16261 gcattaccgt ccgcgtaccg ccgtgttgaa caatctggaa ttcgaccacg ccgacatctt
    16321 cgccgatttg ggcgcgatac agacccagtt ccaccacctc gtgcgtaccg tgccgtctga
    16381 aggcctcatc gtctgcaacg gacggcagca aagcctgcaa gacactttgg acaaaggctg
    16441 ctggacgccg gtggaaaaat tcggcacgga acacggctgg caggccggcg aagccaatgc
    16501 cgatggctcg ttcgacgtgt tgcttgacgg caaaaaagcc ggacacgtcg cttggagttt
    16561 gatgggcgga cacaaccgca tgaacgcgct cgccgtcatc gccgccgcgc gtcatgccgg
    16621 agtcgacatt cagacggcct gcgaagcctt gagcacgttt aaaaacgtca aacgccgcat
    16681 ggaaatcaaa ggcacggtaa acggcatcac tgtttacgac gacttcgccc accatccgac
    16741 cgccatcgaa accaccatag aaggtctgcg tcagcgcgtc ggaaacgccc gcatcctcgc
    16801 cgtcctcgag ccgcgttcca acacgatgaa gctgggcacg atgaagtccg ccctgcccga
    16861 aagcctcaaa gaagccgacc aagtgttctg ctacgccggc ggtgtggact ggaacgtcgc
    16921 cgaagccctc gcacctttgg gcggcaggct gcacgtcggc aaggatttcg atgccttcgt
    16981 tgccgaaatt gtgaaaaacg cccgaaccgg cgaccatatt ttggtgatga gcaacggcgg
    17041 tttcggcgga atacacacca aactgctgga cgctttgaga tagcccgggc gatgccgtct
    17101 gaaagccctt cagacggcat cgcccggctg cgcggcacaa aggcggaaaa accgtttgcc
    17161 ccgtattttc aaacgcgtta cacttgccgc cgctgttttc agccatttga ttacccgcaa
    17221 ccgccgtcat tgcgccggcg gtttgcctgt cagcgtcatt gcgccgctgt aaatacgaaa
    17281 gaacacatta tgaccgtatc ccccgtcgcc ttgcgccgta agaccgagtg caagcctcat
    17341 cccaccgcgc gctattggaa aaaatgcgat gtcgaggcac ttttcggact gccgttttta
    17401 gaacttgtcc atcaggcggc agaagtccac cgccaaaatt tcaacccgcg cgaaatccag
    17461 ctttccacgc tgttgtccat caaaaccggc ggttgtcccg aagattgcgc ctactgtccg
    17521 caatcggcgc accacaatac caatctgggc aaagagcaga tgatggatgt ggatgaaatc
    17581 gtcgaaaaag ccaaaatcgc caaatcgcgc ggcgcaagcc ggttttgtat gggcgcggca
    17641 tggcgcggcc ccaaacccaa agacgtggag acggtttccg caatcatcaa agccgtcaag
    17701 ggcttgggta tggaaacctg cggcacgttc ggtatgctcg aagaaggtat ggcggaagac
    17761 ttgaaagagg cgggcttgga ttattacaac cacaacctcg acaccgaccc cgaccgctac
    17821 aacgacatca tccatacacg ccggcatgaa gaccgtatgg acaccttggg caaagtccgc
    17881 aacgccggtt tgaaagtctg ctgcggcggc atcgtcggga tgaacgaaac tcgtgccgaa
    17941 cgcgccgggc tgattgccag cctcgccaac ctcgacccgc agcccgaaag cgtgccgatt
    18001 aaccggttgg tcaaagtgga aggcacgccg cttgacgatg ccgaagattt ggactggacg
    18061 gaatttgtcc gcaccatcgc cgtggcgcgg attacgatgc cgcaaagtta tgtccggctg
    18121 tcggcagggc gcagcaatat gccggaagcg atgcaggcga tgtgctttat ggcgggcgcg
    18181 aattcgattt tttacggcga caagctgttg acgacaggca atcctgatga ggacggcgac
    18241 agaatcctga tggaaaagct taacctgtat cccttacagt ttgagctgga aggcgaggtc
    18301 gccgaagtgg aaaaagcctc tgggattaaa gtggattatt gacgattgca aaaatgccgt
    18361 ctgaaacccg gaaaaaggct ttcagacggc atttgttcgg acggtatttc caatatcttt
    18421 ttaccggcgc gtgatgttgc cgtcgggcga gacatccagc ccgttcccgt tggggaaatc
    18481 gctgcggttc agataaataa tccgcccgat aacggttttc tcggggtcga ttttggggat
    18541 tttatcgtcg gcttgggtaa tagggatgat ggttccggaa ataaatttac cctgtttgtc
    18601 ggtgatgatt ttgaaaatgg gggcgatgcc gctgatgccg gaggtgttga ttgcgccgta
    18661 ggtggcaaag ttgccgccgc tgtaagagat gaagcggtcg cggtaaagtt cgacggcgcg
    18721 tgtaacgtgc ggcccctgcc cgaatacgac atccgcgccg gaatcgacgg caagccgtgc
    18781 aaactcgacg acgttgcccc tgttttcccc atagaagatt tcggtatcga acggcaggtg
    18841 ttccgcctgt ttcccttccg cgccgccgtg gaacatcaca atgacgatgt cggctttctg
    18901 tttggttttg gtaatccgtt ttctaacttt ggcataatcg ttcagtttga cggcggcaag
    18961 gttgggggcg aaggagacga agccggatct tacgccgttt ttcttcagga tggcggtttc
    19021 aaacctgttt tcgatgcccg aatatttgat gttcaattcg tcaaggttcg ccctttatgc
    19081 cgtttccgca ccgcaaaccg ccggggtcaa gccctcggcg catcctgccg gaacggaatc
    19141 cccgtgctgc cgattgacgg ttcaaacccc gcgcccgttt caaataccgg cgatgtggac
    19201 ggacaggatg cgcctgacga aaagacagcc gataccgttt ccattatcgg cgtgggcgac
    19261 attatgctcg gcagcaatta tccggtcgat tacctgcccg ataccaatat tctgaaaaac
    19321 gtcgaatctg ccttgcaaga cgcggacatt accgtcggca acctcgaagg cacgctgttt
    19381 gacgaaggcg gtacgccgaa aaaatgtgca aacccccaaa atatgctatg cattccgaac
    19441 gccctccgca tacgggcaat accttgccga cgcgggattc gactacctca gcttcgccaa
    19501 caaccacagc aacgacttcg gcgcgcaagg catcacggca acggcggcga gcggcagctc
    19561 ttttacatac tcgatcgcgc taaagccgct gccgataacg aggccaaaat tgcggaaaat
    19621 accgccatcg cctagataaa tttgtccatc atcagacctt tactgttcag acgagacagc
    19681 atttgccgca cgttttgggg cttatctttc gatttgcgct acgtcgcgca ccgcgccttt
    19741 gtcggcggaa gtcgccatcg cgccgtaagc tcttaatgcg gcggagacgt agcggtcgcg
    19801 gtttttaggc ttccatgctt tgctgccgcg cgcttccatt tcggcacggc gtgcggcaag
    19861 ctcttcatcg gaaatggcaa ggtggatgct gcggttgggg atgtcgattt cgacggtatc
    19921 gccttcgtgt accaaaccga tcgcgccacc ttccgccgct tcgggcgagg cgtgtccgat
    19981 ggacaaacct gatgtgccgc cggagaagcg tccgtcggtt aagagagcgc aggctttgcc
    20041 gaggccttta gatttcaggt aggaagtcgg atacagcatt tcctgcatgc ccgggccgcc
    20101 tttcgggcct tcgtagcgga tgatgacgat gtcgccggcg acgatttggt tgcccaaaat
    20161 gccttctact gcgtcttctt ggctttcaaa cacgcgggcg cggccggtga atttgaggat
    20221 gctctcatcc acgcctgcgg tttttaccac gcagccgcgt tcggcgatgt tgccgaacaa
    20281 gaccgccaaa ccgccgtctt gcgagtaggc gtgtgccacg tcgcggatac agcctttttc
    20341 gcggtcgagg tcgagggttt tccacatacg gttttgcgag aacgcttggg tggtacgtac
    20401 gccgccgggc gcggctttga agcgttcgat ggcgcgggtg ttttcagggt tggttacgtc
    20461 ccatttttca atcgcgtctt tcagcgtcgg tgcgtggata gtgtgcacgt cggtgtgcag
    20521 tttgcccgct ttgtccaatt ctttcaggat ggcgaagatg ccgccggctc ggtgcacgtc
    20581 ttccatatag tagtcgtggt tgttgggcgc ggttttgcag atgcagggca cgacgcggct
    20641 taagcggtcg atgtcggcca ttttgaaatc cacgcccgct tcgttggcga cggcgagcaa
    20701 atgcaggatg gtgttggtag agccgcccat cgcaatgtcc atcgtcatag cgttttcaaa
    20761 cgcttttttg gtggcgatgc tgcgcggcag tacggtttcg tcgtcttgct cgtaatagcg
    20821 tttggtgatt tcgacaatca tacggcctgc ttcgaggaac aattctttgc ggccggcgtg
    20881 ggtcgccaaa tacgaaccgt tgccgggcag ggaaaggccg agtgcttcgg tcaggcagtt
    20941 catcgagttt gccgtaaaca tacccgaaca cgagccgcag gtcgggcagg cgttttgttc
    21001 gacttcttcg acttgccggt tgctgacatt gtcgtctgcc gattcgatca tcgcgtcaat
    21061 caagtccaaa cggcgttcgg gttggatgtt tgccacgccg ataactttac ctgcttccat
    21121 cgggccgccg gagacgaaga tggtggggat gttcaggcgc atcgcggcaa tcagcatgcc
    21181 cggggtgatt ttgtcgcagt tggaaatgca caccagcgcg tcggcgcagt gggcgttgac
    21241 catatattcg atagagtcgg caatcaaatc gcggctgggc agggagtaca gcatgccgct
    21301 gtgtcccata gcgatgccgt cgtcgatggc gatggtgttg aattctttgg cgattgcgcc
    21361 ggctttttcg atttcgcggg caaccagctg gcccatattg tgcaggtgga catggccggg
    21421 cacgaattgg gtgaaggagt tggcaacggc gatgatgggc ttgccgaagt cggtttccat
    21481 cacgccggtc gcacgccaca atgcgcgtgc gcctgccata ttgcggccgt gggtagaggt
    21541 tttggagcgg tattctggca tagtgtgttt ccttgtgcct ataccgtctg aaagacaggg
    21601 ctgtttcaga cggtatcggg tacggttttt tagagtggga aaagagggta ttttatacca
    21661 agtatcggaa ttttgcggga ttgaaacggc gtgcggcaaa aaagaaaatc cccgcaggaa
    21721 tgcggggacg ggttcaggcg cgggcaatcg cgacggcttt ggatacgtcc caaaaatcaa
    21781 cgggtgcatt taatacgggt ttgacgatgc ccgtccgtat ggcgaaatcg ccgaaacctt
    21841 cgccgatatt gcgttctgcc gcccatttgc cgatcaggtc gtccaattcg gcaaggattt
    21901 cgggcagggt gatgttttct ttataaaggc gggggatgcg tacgccttca cggtcgccgc
    21961 cgatatggag gttgtagcgt tcgacggctt tgccgaccag cccgatttcc gccaacattg
    22021 cccgtccgca gccgttgggg cagccggtaa tgcgggtaac gatgtagtcg tccgacgtgc
    22081 cgtgtttcgc cataatctta tccagctcgt cgatgaagtc cggcagcacg cgttcggctt
    22141 ccgccattgc cagcgggcag gtcgggaagg aaacgcacga catcgcattt tcgcgcagtt
    22201 tgctgacatc gttgcggatt aatccgtatg ttcgggcaag ttcttcgatt tttgctttgt
    22261 ctgcttcggc gacatttgcc acgatgaggt tttggttggc ggtaatgcgg aaatcgcctt
    22321 tgtggatttt ggcgatttcc aacacgccgg tcagaagctg tttcccgcct tcgtcaacca
    22381 aacggccgct ttcgatgaaa agggtcaaat gccagttgcc gtctatgcct tttacccagc
    22441 cgatgcggtc gccgcgcccg gtaaatttga acgggcgtat gggttcgaac ggcataccca
    22501 tacggcgttc gacttcggcg cggaagttgt ccaagcccat attttgaatg gtgtagcggg
    22561 tgcgggcgtt tttgcggtcg ctgcggttgc cgaagtcgcg ctgtgtggtt accaccgctt
    22621 cggcggcctt cagcgcgtgt tccggaggca cgaaacccag ttccagtgaa atgttcggat
    22681 aggttttggt gttgccgtgt tccatcgaaa gcccgccgcc cgccaaaaca ttgaatccgg
    22741 caagctgtcc gttgccgtct gaaatggcga caaaatccaa gtcgttgccg tagcagtcca
    22801 catcatttaa gggcgggatg acgactgcgg ttttgaattt gcgcggcaga taggttttgc
    22861 ccaaaatcgg ctcgtcttct tgaaggaagt cgtcggaact ttgaactttt ttgccgtcca
    22921 cccaaacatc cagataaccg cgcgtgcgcg gcagcaagtg ttcggaaatc tttttcgcgt
    22981 attcgtaagc ctgccggtgc agttcggact cgatcgggtt ggacgtgcaa agcacgttgc
    23041 ggttcatatc cgccgccgtg gcgatggaat ccaaacccag tttgtgcaag aggcggtgca
    23101 tcgtctgcaa cttggctttc ggcacgccgt gaaattggaa ggtttgccgg ttggtcagcc
    23161 ggatggagcg gtaatgactg ttttcccggg caaatttgtc cagttctatc cattgggacg
    23221 gtttaatgat cccgcccggc agccggcagc gcaaaagcat aaatttcaag ggctcgagtt
    23281 ttgcctcggc gcgttcggcg cggatgtcgc ggtcgtcctg ctcatacata ccgtggaagc
    23341 ggatgagttg gaagttgtcg cctttgaagc cgcccgtgag cgggtctttc aaatcgtcca
    23401 aaatcgtgcc gcgtaaaaaa ttgctttcgg ttttcagacg ttcgttgtcg gatagcggtt
    23461 tttcttgcca cgccaaacct tttgtcttgg tctgtacggt cattttgtgt tcctcccgat
    23521 tatatttaat caataaacat cacgctgata gcgtttttct tcgcgcagca tatccaaata
    23581 ttcttctgcg ccctcttcgt ccaaatgtcc tgccccgata atcacatcca gcaaggcggc
    23641 ttccacgtct tttgccattt ttgccgcatc gccgcacaca tagatatgcg cgccttcctg
    23701 cagccattgc caaagtcctt ccgcctgttc gcggattttg tcctgcacat agattttttc
    23761 ttcctgatcg cgggaccagg cgaaatcgta cctgtgcagg aagccgtctt tggcaaactg
    23821 ctgccattcg gtctgataga ggaagtcggc ggcaaaatgc ggattgccga aaatcagcca
    23881 gtttttgcct tccgcatttt ctgcggcacg ttgttggacg aaagcgcgga acggtgcgac
    23941 gccggtgccc gagccgatca tcacaatcgg cttgcggctg tcttcgggca gcctgaagcc
    24001 gtcgttgcgt tccacaaaca cgcgcaccgt gccgtcctct tccagccggt cggcaaggaa
    24061 acccgatgcg ccgcccaccc tggcgcgccc ttcgtgttca aaacgcaccg cgccgacggt
    24121 caggtgcact tcgtccccca cttccgcctg cgacgaggaa atcgaataca ggcgcggcgc
    24181 aagcgggcga agcaggccgg cgaattgttc cgccgtcagt tttgccggga agcggtgcag
    24241 cacatcggca atcggcgtgc tttgcacaaa gccttgcaaa acggcgttgt cggcagcaat
    24301 acggtcgagt tcgtcatcat cggcgaacgg ggcatagcct ttgacaaagg cgggggtgtt
    24361 ttgcgtgagt tcaaaatggg ataacagtgc ggaggcaacc ggcagggttt ttccgcccgc
    24421 ctgtatttcc gttgccggat cgatgccgag caggtctagg atttccctga ccagtgccgg
    24481 atcgttgtca aaccaaacgc cgagcgcgtc gcccgggagg tagtgcaaat ccgaaccgct
    24541 caaatcgatt tcgatgtggc gcacgtcttt atcggattgg cgggcggtga ttttctgatt
    24601 ggccagcagg gcggcgggaa aggggtctgc cttgcagtac ctgccctccg atgccgtctg
    24661 aaggccggcg gggggcgttg cctgcggcgc gggcgttgcc cggttttttg cggcttcttc
    24721 ttttaagagt gcggcgatat tgtctgtcca ggcgtttgcg gcggcggcaa agtccaaatc
    24781 cgcatcaacg cgttcgagca ggcgtttcgc ccccaattct tcaaaacgtt tgtcgaaatc
    24841 tttgcccgcc cggcagaaat tcggatagga actgtcgccc aaacccagta cggcaaattg
    24901 gagtttgtcc aatttcgggg cttttttgcc gttcagcagt ttgtgcagca cgacggcttc
    24961 ttccggcggt tcgccttcgc cttgggtgga cgtaaccagc agcaggcggc gttcgccggc
    25021 gatgtttttc gccttatagt ctttcagttc ggcgcgactg acttggatgc cggcggcttc
    25081 caggctgtcc gccgctttgt cggcaacgga tttcgcattg ccggtttgcg aggcggaaag
    25141 gacggttacg gaaaaaggtt ctgccgtcgg caatgccgtc tgaagcgcgg gcagtcctgc
    25201 agatgccccg tttcctgctt ttgcccaagc gtagccggac agccacgccc attgtgccgc
    25261 gtccagcccc gacaggagct gcgtgatttc gggcggcaga ggcggtaatg gcggatttgt
    25321 gttctgcata tcgtgttcac tcataaaatc atacctgccg caacagtgcc gtatgtcgct
    25381 tcgtctatca ggataaacga accggcggcg gtgttttccg cataaggcgt tgccgtaacg
    25441 ggtttttgaa ggttgatgcg gactttggcg atgtcgttca tcttcaagga ttccgcgccg
    25501 gcctcttgtt ccagcgtgcg gacatccaaa acgctttcaa tttccccgac ttttgccggc
    25561 acggtttgcg tgccgtgctt gagcaggtat ttgcgcgcgg tgttgagcgg acgttcgtca
    25621 aaccagcaaa gcgtggcttc cagatgtttt tgcggggcga gcggggaatt tttatcgaca
    25681 aaaaggtcgc cgcgcgaaac atcgatgtcg cggtccagcc ggagggttgc cgcctcgccg
    25741 gcaaaagcct gcgccacttc ccctttcggc gtgatgattt cggacacttc ggcggtcagc
    25801 ccgttcggtt cgatgcggac ggtttgcccg acggcgaccg aaccgcgttc gatgcgcccc
    25861 tgatagcctc ggaaatcatc ggccttgtcg gcatcttggc ggacgaccag ttgcacgggg
    25921 aaataaaaat cgtcggcggt gcggctgact tcgtccgccc ccggcaggtt ttccaaaatg
    25981 gacaataagg gttcgccttt ataccaaggc atattgccgc cggggtaaac gatgttgtcg
    26041 cccaagagtg cggacaccgg tacgaaatgc acgtctttca aaccgagctg ttcggcaagt
    26101 cggcggtatg cctccacaat ggcgttgaat ttgtcttcgc tgtaatccag caggtccatt
    26161 ttgttgaccg ccaccacaat atgcgggcag ttgagttggc ggaggatggc ggaatggcgt
    26221 ttggtctgcg gcagaagctg caagggctgc gcgccgaaat ccagttggga tgcgtcaacc
    26281 agcacgactg ccgccgaagc ggtgcttgcg cccgtaacca tattgcgcgt gtattgttcg
    26341 tgccccggcg tgtcggcgat gatgaatttc cgtttcgccg tggaaaaata gcggtatgcc
    26401 acatcgatcg taatgccctg ttcgcgttcg gcttccagtc cgtcggtcag gatggagaag
    26461 tctatggttt ctttcaaacc tttgcttttg ccggattcca aggttttgat ttggtcggac
    26521 agcagggctt tgctgtcgta gagcagtcgt ccgattaggg tgcttttgcc gtcatcgacg
    26581 ctgccggcgg taatgaagcg gagcggggtt tggtgttgtg ccgtcatatt ttcttcctca
    26641 tatctgctta aagggttttt gaaatttaga aatagccttc ttttttgcgt ttttccattg
    26701 ccgcctcgct tgcctgatcg tccagccggg tcgcgctgcg ttcggaaatg tcggcaaccg
    26761 ctgtttctct gataatctcc gtcggcgtgg acgcggtgct ttctaccggg caggtgcagc
    26821 tgatgtcgcc gacggtgcgg aagcggacat caaggatttc ggaggtttca gacggcattt
    26881 tcggggtgag cggcgttacg gggaccagca gccccctgcg tctgaccact tcgcgcctgt
    26941 ggctgtaata aatcggcggc agctcgaggt tttcgcgggc gatgtattgc cagatgtcga
    27001 gttccgtcca gttggaaatc gggaagacgc gcatattttc gcctttgtgc agcctggtgt
    27061 tgtacagcga ccacagctcg gggcgttgcg ccttcggatc ccattgtccg aactcgtctc
    27121 ggaacgagaa aatccgttct ttggcgcggg ctttttcttc gtcgcgccgc gcgccgccca
    27181 taagcgcgtc aaaaccgttt gcctcgatgg tttccaacaa ggtaaccgcc tgtgccgcat
    27241 tgcgcgaatc ggtttctttg cgtaagacca ccgtgccttt ggcaatggag tcttccacgc
    27301 gccccactat caggcgggca ttgagttttg ccgcctgcgc gtcgcggaag gcaatcactt
    27361 cggggtagtt gtgccccgtg tcgatatgca ccagcgggaa gggcagtttt accggccggc
    27421 tgcccagctg gaaggctttg caggcgaggg cgagcaggac cacggaatct ttgccgccgg
    27481 aaaagagcag ggcggggttt tcgcattccg ccgccacttc gcggatgatg tggatggatt
    27541 cggattccaa ccagtcgagt tgggcgttgt tcggttcggt tttcgtcata ccatattcct
    27601 tatttcttct gtctgatatt tatgaattat ttgtgcagcc cgcattcttt gctgtttctg
    27661 ccttcccacc accaccgccc ggcgcggatg tcttcgcccg ccttgacggg gcgggtgcag
    27721 gggtcgcagc ctatgctggg aaatccttgc cggtacaaat cgttgtaagg cacattgttg
    27781 gcgaggatgt atgcccacac gtcgtgttcc gaccagtcga aaatcgggtt gtatttgccg
    27841 atgccccgtc cggcatcgta ttcggcaaac ggcagttccg tgcgtgtggc ggattgttcg
    27901 cggcgttgcc cggtaagcca ggcgtccgcg cctgcaatgg cgcggttgag cggttcgatt
    27961 tttcggatgc ggcagcattc gcggcgcgct tcaacgctgt cgtaaaaggc aaacctgcct
    28021 ttgctttcca cataacggtc ggcatcttct cgaaccggcc ggaaacgctt tatccgcaaa
    28081 tggggatatg cgcgtccgag cctgtccagc aggttcaggg tttccgtgtg gagcagcccc
    28141 gtatccaagg taaaaatgcc gatattgagg ttttcgccgg cgataaggtc ggtaatcacc
    28201 atatcttctg ccgcaaggct gctggcaaac cgtgcatccc ggtggctgcc gacaatccgg
    28261 tgcaggcgtt gtttgagggt ttccgttttt tccgcaaggg cggtttcgcc gccggatccg
    28321 atatgcggta tctgccacag ggcgggtttg aacagtgtcg tttccatttt tcccgcctta
    28381 tgccgcccgt tgtccggcat tcagtccgcc caatgcggga tacgcctgcc cgacccggtt
    28441 ttctccttcg ccgttttcac cgaaccaggc gagtttttcg tgcagcccca ccacttcgcc
    28501 tatgacaatc aatgccggat tcggcgcggt ttcggcgagt tcggcaaggt tggcgagcgt
    28561 gccggttgcg gttttttgag ccggcagcgt gccttggctg ataacggctg ccggcgtgtc
    28621 gggcgagcgt ccgtgctgtt gcagccgttc ggcaatcagg gcggctttga gcgcacccat
    28681 ataaatcacc aaggtctggc ggctgcgggc gagggtctgc cattcgatgt cgggcgcatc
    28741 cgccttgcgg tggccggtta cgaaaaccgc actttgggca taatcgcggt gcgtgagcgg
    28801 gatgccggca taggcggtcg cgccgacggc ggcggtaatg ccggggacga ccgaaaacgg
    28861 gatctgatgg cgtgccaagg tttccaattc ttcgccgccg cgtccgaaca cgaaagggtc
    28921 tccgcctttt aaacgcacca cgcgcctgcc ttcgcgggcc agcctgacca taagcgcatt
    28981 ggtgtcctct tgcggggtgc ggccgccccg ggcgcgcttg ccgacaaaaa tccgttccgc
    29041 atcgcggcgg acgagggaca gtatgccgta tgaaaccagc gcgtcgtaaa gcaccacgtc
    29101 tgcctgctgg atttcctgca gccctttgag cgtcagcagc cccgcatcgc cgggacccgc
    29161 accgaccaaa gacacaaaac cgccttgata attttgttcc aactgctttg ccaattcccg
    29221 ttcggcaagg gtgttttgcc ggtttttgac gagggcggcg aaacgtccgt taaactgctt
    29281 ttcccaaaag cggcggcgtt cggtaacgga tttcagtttg cccttgacgg catcgcgcca
    29341 ccttcctgaa atttccgcca tatcgcccaa agacggcggc agcagggctt ccagcctttc
    29401 acgcagcagg cgggcgagga cgggcgcgct gccggagctg gaaacggcaa tctgaaccgg
    29461 gttgcggtcg ataaccgacg ggaagatgaa gctgcaatgg tcgcggtcgt ccaccacgtt
    29521 gaccggcttt tggcagcttt cggcaagatg gaaaacgcgc cggttgaggg cttggtcgct
    29581 gcttgccgca atgatgagga aaaccgtgcg gatgtgttcg gcacgaaatt cttcggcaag
    29641 ccacaggatt ttgttttctg ccgccagcgc acttaattca gcattcaact gatttgcaac
    29701 aaccctgact tgcgcgccgg catccagcaa caagctgatt ttgcgcgcgg cgaccgaacc
    29761 gccgcctacg accaatacgg ggcggccggc gaggttggcg aaaatgggga aataattcac
    29821 tggcgaactc ctttgctgtt tgcacgcacc ttgtttccga tatggtgcgt cgcggtattt
    29881 ttgtcggaat gcgggtcatt ttagacaaaa ggattttccc ggttaaataa taaaaaggca
    29941 tttgttagaa gctgaaagct atatgagggc ggctgcggat gcggcggttt tccgttttat
    30001 agtggattaa caaaaaccag tacggcgttg tctcgcctta gctcaaagag aacgattctc
    30061 taaggtgctg aagcactaag taaatcggtt ctgtactatc cgtactgtct gcggcttcgt
    30121 ctccttgtcc tgatttttgt taatccacta taaaacggtt tgggacggaa aaacggcctg
    30181 aagccgtttc gggcattcag accgtttgcg cggagagggg atgccgtccg aagggcgaaa
    30241 agggtttcag acggcattga tgtcgggttt caggacagga gcaggatggc ggctgcggca
    30301 agcgcggcaa ccgataatgc ggcggcaagc gcggctttgc cggcaaagcg gattgaggtt
    30361 ttgccttcga tgtatttgaa gccggttatc atcgggagga cgaggttttt ctttttgaac
    30421 acgcggtatg cggcgacggc ggcgatatga atggcggaaa aaacggcgag cagcttgaaa
    30481 aagttgaggt ggattttccg cataaggctg cccgtatgct cggaaaccaa atggttgagg
    30541 tagccgttgg tgctgaaggt gttttcatcg gcggcaaaaa gccctgtgcc gacttgaaat
    30601 gagacggcgg ccaaaagcgc gacgaccatc agtgcgccca aggggttgtg tccgggttgg
    30661 acgtgttcgg gaataccgtt tttcatatac tctctgatac ccgaccatcc gcggacgaaa
    30721 cgggagaaac gggcggtatc gctgccccaa atgccccagc agaggcggaa tacgagcagg
    30781 aaaaggataa acagcccgac gcgcgtgtgc cattgcagca tatcgccgcc ggttttcgcg
    30841 ctataccaca taaagggtag ggatgcggca agcagccagt ggaaaaggcg ggtggggaag
    30901 tcccagactt tgcttttgtt tttcataatc ggtttccggc ggtagaatcg gtttgttttc
    30961 gagccttaat tttaaccgat tggaggggaa atgtttcccg ttttccatct ttcaggcgaa
    31021 agccgccgcc ggatgcttca ggcggcatcg cgttttccct atgttttcaa agcccgtgcg
    31081 gaagattcgc acaaagggac tttcggcacg ctcgccgtag tcggcggatc ggcagggatg
    31141 agcggcgcgc ccgtattggc ggcatcggcg gcgatgtatc tcggctgcgg caaggtgtgg
    31201 gcgggtttca atcaggatgc gcttccgttt gccgtcattg ccggttttcc cgaaattatg
    31261 ttggacacgg cagacggttt gaccaagcgt caaggcataa acgcttggat ggtaggttgc
    31321 ggcctgggta cggataaagc ggcggtcgga acgcttgccg gaattttggc ggaacacacg
    31381 gacaagcccg tccttttgga tgcggacgcg ctgaacatat tatcaaccga tgccgaaacc
    31441 cgaaatctgg cgcgcgggtg taaaaacctg attttaacgc cacaccccgc cgaagccgcg
    31501 cgcctgcttg gaacgacggt tgcacaggtt caggcggatc ggacggcggc agtgaggaag
    31561 ataagggcaa ttttcggcgc aaccgtggtt ttaaagggca caaaacattg gttgcctcac
    31621 ccgatacgga aatctatgtc aacgaaagcg gcaacgcggg attggcaacg gcgggcagtg
    31681 gagacgtatt gggcggcatc atcggcagtc tgcttgcaca gggcgtgccg gtttttgaag
    31741 ccgcctgcgc gggcgcgtgg ctgcacggcg cggcggcgga tgtcataaaa gaatcggcag
    31801 gcattgcggc ggggctgttg gctggcgaaa tcgccccggc ggcaaggtgg ctgcgcaacc
    31861 ggataactga aagtatgtaa gaagatatag tggattaaca aaaaccagta c
//
LOCUS       DAWFIE010000015        31342 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWFIE010000015 DAWFIE010000000
VERSION     DAWFIE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31342
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.015"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(68..814)
                     /locus_tag="WGQ71_000647"
     CDS             complement(68..814)
                     /locus_tag="WGQ71_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0706702.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(993..1664)
                     /locus_tag="WGQ71_000648"
     CDS             complement(993..1664)
                     /locus_tag="WGQ71_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ0706703.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGFDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WGQ71_000649"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WGQ71_000649"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ0706704.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFICIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /locus_tag="WGQ71_000650"
     CDS             complement(2481..2963)
                     /locus_tag="WGQ71_000650"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ0706705.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /locus_tag="WGQ71_000651"
     CDS             complement(2995..3684)
                     /locus_tag="WGQ71_000651"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ0706706.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAVPVADTLKRAEGGQISATVDRSG
                     LWQAQTPQLFRAGLLYRALAAENLDGITDEASAVEKLGVRPLLIQGDVRNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WGQ71_000652"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WGQ71_000652"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ0706707.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGEAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4570..5880)
                     /locus_tag="WGQ71_000653"
     CDS             complement(4570..5880)
                     /locus_tag="WGQ71_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0706708.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMMLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIREKR"
     gene            complement(5870..6055)
                     /gene="ccoS"
                     /locus_tag="WGQ71_000654"
     CDS             complement(5870..6055)
                     /gene="ccoS"
                     /locus_tag="WGQ71_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ0706709.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6055..6354)
                     /locus_tag="WGQ71_000655"
     CDS             complement(6055..6354)
                     /locus_tag="WGQ71_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706710.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVIKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6705..7901
                     /locus_tag="WGQ71_000656"
     CDS             6705..7901
                     /locus_tag="WGQ71_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0706711.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(8415..10256)
                     /locus_tag="WGQ71_000657"
     CDS             complement(8415..10256)
                     /locus_tag="WGQ71_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ0706712.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGVLPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFVLGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANMP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            10347..10817
                     /locus_tag="WGQ71_000658"
     CDS             10347..10817
                     /locus_tag="WGQ71_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ0706713.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     CYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKHGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAENQARLNPHEIA"
     gene            complement(10890..11702)
                     /gene="hpnC"
                     /locus_tag="WGQ71_000659"
     CDS             complement(10890..11702)
                     /gene="hpnC"
                     /locus_tag="WGQ71_000659"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ0706714.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLQRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(11789..12271)
                     /locus_tag="WGQ71_000660"
     CDS             complement(11789..12271)
                     /locus_tag="WGQ71_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0706715.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            12744..13025
                     /locus_tag="WGQ71_000661"
     CDS             12744..13025
                     /locus_tag="WGQ71_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706716.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPAAEAPAAEAPATEAPAA
                     EAPATEAPAAEAPAAEAPAAEAPATEAPAAEAAATEAPAAEAPAAEAAK"
     gene            13135..14502
                     /locus_tag="WGQ71_000662"
     CDS             13135..14502
                     /locus_tag="WGQ71_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0706717.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            14537..15043
                     /locus_tag="WGQ71_000663"
     CDS             14537..15043
                     /locus_tag="WGQ71_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HEZ0706718.1"
                     /translation="MGMTATYQLVDNEKLALLKNTDTEWQELFEELEDLEDEAAVCLD
                     IDKMWDVLHFVLTGTSAENPLKGNLLSESIVGTAPISDDDGYAAYIESAQIHEIISAL
                     EGFDIKSAMEHFSMQVCKEHHIYPDIWDYEDEIYDIKEEITDYFYKIGNFYKTARHSN
                     SNVLVSIY"
     gene            complement(15471..15917)
                     /gene="smpB"
                     /locus_tag="WGQ71_000664"
     CDS             complement(15471..15917)
                     /gene="smpB"
                     /locus_tag="WGQ71_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ0706719.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(15972..16982)
                     /gene="waaF"
                     /locus_tag="WGQ71_000665"
     CDS             complement(15972..16982)
                     /gene="waaF"
                     /locus_tag="WGQ71_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ0706720.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPAKHFAELGKHYSATGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(17113..17188)
                     /locus_tag="WGQ71_000666"
     tRNA            complement(17113..17188)
                     /locus_tag="WGQ71_000666"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17153..17155),aa:Asn,seq:gtt)
     gene            complement(17379..18131)
                     /locus_tag="WGQ71_000667"
     CDS             complement(17379..18131)
                     /locus_tag="WGQ71_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ0706721.1"
                     /translation="MITLPSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSVLIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            18442..18966
                     /locus_tag="WGQ71_000668"
     CDS             18442..18966
                     /locus_tag="WGQ71_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ0706722.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVHRRN
                     ATSLQLSRCANIADDQSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSATSA
                     ALCGTSSSVSTAAA"
     gene            complement(19055..19334)
                     /locus_tag="WGQ71_000669"
                     /pseudo
     CDS             complement(19055..19334)
                     /locus_tag="WGQ71_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(19706..20851)
                     /gene="dapE"
                     /locus_tag="WGQ71_000670"
     CDS             complement(19706..20851)
                     /gene="dapE"
                     /locus_tag="WGQ71_000670"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ0706723.1"
                     /translation="MTETQSLELAKALISRPSVTPDDRDCQKLLVERLYKIGFAAEEL
                     HFGDTKNIWLRRGTKVPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNKYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            complement(20965..21585)
                     /locus_tag="WGQ71_000671"
     CDS             complement(20965..21585)
                     /locus_tag="WGQ71_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ0706724.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(21619..22128)
                     /locus_tag="WGQ71_000672"
     CDS             complement(21619..22128)
                     /locus_tag="WGQ71_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0706725.1"
                     /translation="MNLFETQSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQLVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(22224..22775)
                     /gene="azu"
                     /locus_tag="WGQ71_000673"
     CDS             complement(22224..22775)
                     /gene="azu"
                     /locus_tag="WGQ71_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ0706726.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKTSMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(23056..23478)
                     /locus_tag="WGQ71_000674"
     CDS             complement(23056..23478)
                     /locus_tag="WGQ71_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ0706727.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            23609..26359
                     /gene="secA"
                     /locus_tag="WGQ71_000675"
     CDS             23609..26359
                     /gene="secA"
                     /locus_tag="WGQ71_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ0706728.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            26503..28275
                     /locus_tag="WGQ71_000676"
     CDS             26503..28275
                     /locus_tag="WGQ71_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ0706729.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLRFNPTAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEVGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLTDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            28462..30390
                     /gene="rpoD"
                     /locus_tag="WGQ71_000677"
     CDS             28462..30390
                     /gene="rpoD"
                     /locus_tag="WGQ71_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ0706730.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            30558..>31342
                     /locus_tag="WGQ71_000678"
     CDS             30558..>31342
                     /locus_tag="WGQ71_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0706731.1"
                     /translation="MIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRIRYVRDHRGRPAY
                     PLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYSTLCRYRNWLAQ
                     DDTLSELLKLINRQLTEKGLKIEKASAAVIDATIIQTAGSKQRQAIEVDEEGQISGQT
                     TPSKDSDARWIKKNGLYRLGYKQHTRTDAEGYIEKLHITPANTHECNHLLPLLEGITE
                     GTTIYADKGYDSAENRTSGRTSVVRRHYARAAYFGLIKVSAQS"
BASE COUNT         7993 a         9114 c         7398 g         6837 t
ORIGIN      
        1 ataaatttac cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatacggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctaa ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa accttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaaga aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccataaaccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg cccggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtgtcg aacacgcgga cgctgacttt cctgccttcc
     1321 gccgccgcgc gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc gaaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacaaggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatatcgga atcggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tatgccgttt tcgagcacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggctg atcgatattc aagccgtgga catcgacgat ttggttgccg taaaacgtgg
     1921 tgtagccgat acgcagttcg ggctgtccgc ccatagcgag cagtttgcgc gaaacaagag
     1981 agcgcgcgct ttcgacgact tccacaggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcatc cgcaatgcag atgaattttt cggacgcgct ggcgacgatt ttctcgttga
     2101 gatgcgcacc gccgccgcct ttaatcattt gcagggcgtg gttcacttcg tccgcaccgt
     2161 cgatatagac cgccaaaccc gatacttcgt tcagggaaac gacgggaatg tcgtactggg
     2221 caagcagttc gctggatttt ttggaagtag ataccgcgcc tttgattttt ttaccgcttt
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgaaccggt accgatgccg atgtattcgt
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatccgt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatgc gcccgagata gccgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcgccaag cagcgcgtcg
     2821 gtaacggcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcaaac
     3001 ggcatcgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cacgtcgccc tgtatcagta gcggacgcac acccagtttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtac agcagcccgg cgcggaaaag
     3181 ctgcggcgtt tgcgcctgcc aaagccccga acggtcgaca gtggcactga tttgtccgcc
     3241 ttctgcgcgt ttgagcgtat cggcaacggg aactgccaaa attccgcctt cggcagcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcaac gcgcggcatc
     3361 gtgtaccaga atattgtcgg tttccgccgc caaaccggtt tccaacagtt ttgccacacc
     3421 gttgcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaat cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgct tcatgcgtcc gtcccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgcgc cgccaactcg
     3781 ttttcgtccg ccctgatgac tttcagtttg ccgctgcgtt tggtttcggt atgcaccacg
     3841 ggtttggttt ccattttttc ctctgcggcc tcccccatca ggtcgaactg ccgccgcgtc
     3901 atagcaagat agacttcgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac aaggcatcca ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctctatc gtcagaccgt gcaccctcgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcca gaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tatttcggtg caggctgttc
     4561 agacggcatt taccgttttt ccctgattgc cgccgcgcct aaaaaccgcc tttcgcgctt
     4621 aatcaaaaat accgacaacg gaatattgcc caaagcgaca atcagataca acaaggaaat
     4681 gctgtcaaac aaaaacagca acaccgcgct caaaacggcg gcggaaacca taaaaatacc
     4741 gttaacgata ttgttggcgg caacggcgcg ggcgcggaaa gtttcgctgc tggcggtttg
     4801 cagccaggta tagagcggaa cggagaaaaa tccgccgaaa aagccgatca gcatcatcac
     4861 cgccatcacg gggtatgccc atccttgcga taaaaaccaa aaaatgccgt tcagcccttc
     4921 aaaacggtgt ccgtgcgtca gccacaccaa aaccagaccg caaaccgtca aacccaacgc
     4981 accaaccgtt acccaagcca acatcagccg ttccctgctg aacttggcac acagtaccga
     5041 accggcggca ataccgatgg aaaacagggc aagcatcagg ttgaaaacat tgtcgttgcc
     5101 gcccagatgg atttgggtaa aggtcggcag ttgcgtggta taaaccgcgc cgacaaacca
     5161 aaaccacgaa ataccgataa tggcggtaaa aacgggcttg tgccgcaccg tttcacgcag
     5221 cagggatttt gtgccacgga caatattcca ctcaatttgg gtatcggcag ccttggcggg
     5281 tacagacggc ataaacaggc tgccgaccgt gcctccgacg gcgaccagca aaaccagtat
     5341 cccgacaata taaggcggta cacctgccac cgccgttccc aaaatctgac cgaacaggat
     5401 ggcgacaaac gtacccgatt caatcaggct gttgcccatc atcaactctt tgtcgtcgag
     5461 ataatcgggc aggatggcgt atttcagcgg cccgaacagc gtcgattgcg cgcccatgca
     5521 aaacagacac gccaaaagca gcggggcaga ccggatataa aacccgtatg ccgccaccgc
     5581 cataatgatc atttccagca ccttgaccca acgcgccaaa acggccttgt cgaatttgtt
     5641 acccaactgc cccgacagcg aggaaaacag gaaatacggc aaaataaaca acaacgcgcc
     5701 caagttcaac atctgtccgg caggcaggaa gccgttttgc cccaaaccgt aaaacccaat
     5761 catcacaaac agcgcggttt tgaacacatt gtcgttgaac gcgccgagaa actgcgtagc
     5821 gaaaagaggt gcgaaacggc ggcttttaac cagtcccaaa ccgccttttt tagcgtacat
     5881 cgttttccct ctctttttca atcagtttac ttgtcgaatc atcatccatc aggatgcggt
     5941 gcgccggccc ttccaagtcg tcaaactgcc cgtttttgcc cgaccaccaa aaaaaccagc
     6001 cgatgacaaa cgccaaaata atgctgatgg gcaccaatat aaacatgctt tccatcacat
     6061 attccctgtc aaatcgttca aaacaaaagt ctgccccgac acggtcagat attcgttacg
     6121 caaagttccg acgggagctt cgtcaaaaaa cagctcgata cggtctttga tcacgcgcca
     6181 atattggggg atttccgtct gaccgaacgg cgacaggaca tgattttcca ttccgccttc
     6241 aagtttgacg gcaaaacgcc cgctttgcgg ccgtgcttcc gattcgtcgt cggcaagcag
     6301 gatgaaaaag cctatatgcc gtcccgattg gtcatgaata ctgaaataat gcataaattt
     6361 cccacccgcc ttttttcaga cgacaccaac taaaaacagg gcgaatgtac cagtttggat
     6421 gggaagaatg caaagaaatt ctccctcccc cagccgaaaa caccggcaaa ccgcatatcc
     6481 cccttttttc cgtcaaaata cccgacttcc gccattttca cgcaaacgcc cgattaagcc
     6541 aagcaattgc aaagattttt tgttagaata gcctgcttct tttatcaacc ttttcagacg
     6601 gccccactac tttcccgccc aggaaggcaa aacggattcg gcacgaatcc ggttagtatc
     6661 cgtgtccgat tccaatgccg tctgaaactt tccggagtaa gaaaatgtcc caaaaattga
     6721 tcttggtttt gaactgcggc agctcgtccc tcaaaggcgc ggtcctggat aacggcagcg
     6781 gcgaagtcct gctcagctgc cttgccgaaa aactcaacct gcccgatgcc tacatcacat
     6841 tcaaagtaaa cggcgaaaaa cacaaagtcg atctgtccgc acatcccgac cacaccggcg
     6901 cggtcgaagc cctgatggaa gaactcaaag cccacggcct cgacagccgc atcggcgcca
     6961 tcggccaccg cgtcgtcagc ggcggcgaac tgtacagcga atccatcctc gttgacgacg
     7021 aagtcattgc cggcatcgaa aaatgcatcc cgctcgcccc cctgcacaac cccgcccacc
     7081 tcttgggcct gcgtgccgcg caaagcattt tcaaagacct gcccaacgtc gtcgtattcg
     7141 atacctcctt ccaccaaacc atgcccgaag tcgcctacaa atacgccgtt ccgcaggagt
     7201 tgtatgaaaa atacggcctg cgccgttacg gcgcgcacgg taccagctac cgcttcgtcg
     7261 ccgacgaaac cgcgcgcttc ctcggcaaag acaaaaaaga cctgcgtatg gtcattgccc
     7321 acttgggcaa cggcgcgtcc atcaccgccg tcgccaacgg cgaatcgcgc gacaccagta
     7381 tgggcctgac cccgctggaa gggctggtaa tgggtacgcg cagcggcgac atcgatcctt
     7441 ccgtattcgg cttccttgcc gaaaacgcca atatgaccat cgcccaaatc actgaaatgc
     7501 tgaacaaaaa atccggtctg ctcggcattt ccggcctgtc caacgactgc cgcaccattg
     7561 aagaagaagc cgccaagggg cataaaggcg cgaaattggc cttggatatg tttatctacc
     7621 gccttgccaa atacatcggc agtatggcgg ttgccgcagg cggtttggac gcactggtct
     7681 ttaccggcgg catcggcgaa aactccgaca tcatccgcga acgcgtgatc ggctacttgg
     7741 gcttcctcgg tctgaacatc gaccaagaag ccaacctgaa agcccgcttc ggcaacgccg
     7801 gcgtgattac cactgccgac agcaaagccg ttgccgtggt cattccgacc aacgaagagc
     7861 tgatgattgc ccacgacact gcccgtttga gcggtctgta aggttttatc cgcacacgaa
     7921 ctgcctccgg aaatggaggc agttttttat ccggctttcc atgcttaaac agcactgcct
     7981 cttttcagac attgacggtt gcagccgctt acctgaacct tatagtggat taaatttaaa
     8041 tcagtacggc gttgcctcgc cttgccgtac tatctgtact gtctgcggct tcgtcgcctt
     8101 gtcctgattt aaatttaatc cactataatg attgactatt ttttaatcat gttattattt
     8161 tccataaaat acatgacatt aagatgtttt tccacaaaag atacacacac cggcaaacac
     8221 cggctgtgtt tatcttttct tatgcctatt ttttaatcat cgtattttta tcttttaatt
     8281 tcaatacgca aactaactta tacacacggt tttcacatct ttggactgct tccgtgtgta
     8341 tagtggatat tgccgttttc ctttctgaca aaaatgccgt ctgagaactt cagacggcat
     8401 ttgaaacatc ggaatcagcg gttttgttca taccactcga taaacttgtc tgctttgaca
     8461 aaacccagca gcggctcgct gcggctgccg tcggagcgga cgacaaacac gcccggcggc
     8521 ccgaacagac cgtattcttt caacaacgcc tgatgttcgg gcatgttggc ggttacatcg
     8581 atttggaaaa agcgttccat atcgactgcc tgatgcactt ccggctgatt gagcgtgtaa
     8641 gccgccattt ctttgcagga aatgcaccag tcggcataaa aatccaaaac gacgggtttg
     8701 tcgggatgtt ctttcaacgc cgtatccatc gctgccttca gcgcggcagt atcggcaaac
     8761 atttttccgt gttccgaaga tttgcctgct tcagccggcg gattgagggt caggaaatgg
     8821 tgcagcgcgg tcgttttgcc gtttgcgccc tgccagccga accacgcgcc gcctatcagc
     8881 aatatgccgc ccaatacgaa tgccacagct ttcggacggc gtttctgcct gcgtccgttg
     8941 accagcagca tacaggcagg aaccagcatc agcagcgtgt acagcgcgac gacgagataa
     9001 tagggcaagt gcggcgtggc gaggtaaacg gcgacggcaa gcaggataaa gccgaaagcg
     9061 tatttgacgg cattcatcca atcgcctgcc ttaggcagga tatgcccgcc gaacgtgccg
     9121 atggcaatca gcggaacgcc ggtgcccaac gccaaagtgt aaagtgccaa accgcctaaa
     9181 accgcatcgc ccgtctgacc gatgtagccc aaagcaaatg ccagcggcgg ggcgacgcac
     9241 ggcccgacaa tcagcgcgga caatatgccc ataataaaga cggaaacgat tttaccgcct
     9301 gaaagcctgc tgctttgatt ctgaaaatac gactgcacgg cgttgggaag ctggatgttg
     9361 aacagcccga acatagacag tgccaagacg accattaaag ccgatgccgc caacaccacc
     9421 caagcctgct gcaaccatac ggtcagcagt gcgcccgtca gtccggcaac aatgccgacc
     9481 agcgtataag tcagagccaa accctgaaca taaacgacgg acagcacaaa cgcccgcgcc
     9541 ttgcccgcct ttttgtcgcc gaccacaata ctggaaacaa tcggcaacag gggatacata
     9601 caggcggtaa aactcagacc caaaccggcg agaaaaaacg ccaaaagatt ggcgttgagc
     9661 gtatcccaag acagcttgaa acggctgtcg ccgccctcat cccccttcgg gggcggcagc
     9721 accccgctgc cgttttgaga ggaaggctgc aaaaagcggt ctttggcgga tgccggttcg
     9781 tcggtttgcg gatggtaagt gccgttgccg gaaatatcaa actcggtatc cacgggcgga
     9841 tagcacacgc cggcttcggc acagccctga taggtcaaaa ccaatttata cggttcgccg
     9901 acagcctttg cataaggaaa ggcaacctgc gcttcgtgat ggtaaaccgt ctgcctgccg
     9961 aaaaactcgt cttccttctc ttcacccttg ctgaaagaag gctgtcccaa caaatccgcc
    10021 ggatcggtct tgccgacgat tttcgcctga tacatatagt atccgtcggc aatcctgaaa
    10081 cggacgttca caccgtcgtc ggcaacggca agctccggca cgaatgcctt ttccggcggc
    10141 agcagatcgt tcgcatccag cgcgaaagct cgtccgcaca acatcaaaaa tacggcgaac
    10201 aggcaaatca gttttttcat aatcgaatcc gtttcagaca aataatttgt ctgcattata
    10261 aatggtaagg ttgacggtgg gatttaattt atgtaaaacc cgccattatc cgaacctatt
    10321 tccataaaca tcttatcgaa cccgccatgt acgatgtcaa tacccacgat gtccgccgct
    10381 ttttcgcccg cgtgtggcag cagcggctca atccgctgca actgagcgca ctggaacaga
    10441 aagccctccg cattgtcgaa gcccatcccg aataccactg ctatctcgaa cgcatcgaag
    10501 accatctgga caccgactgg ctgcccgaaa acggcgaaag caaccccttc ctgcatatgt
    10561 cgctgcattt gtccgtccaa gaacaggcgg gcatagacca gccgcacggc atacgcgcaa
    10621 tccacgacac cctgtgcgcc aaacacggct ggctggaagc cgaacacgaa atgatggagg
    10681 cactggcgga aacactgtgg acggcgcaac gctacggcac cggtttggat gtcaatttct
    10741 acatgacccg actgcgcaaa ctcatcggct tgggtgcaga aaatcaagcc aggttgaacc
    10801 cgcatgaaat cgcctgacca taccaaccgc ctgcaaaatg ccgtctgaag cggaacaacc
    10861 cctttcagac ggcattcatt ttcccccaat catttccaca acgccttttt cagcataatc
    10921 aaccaatcct tcttatccaa aacggggcgt tgtgcaaaca catcgtatcg gcacgcgtcc
    10981 agtttctgca aaatcaactg cgcccccaac acaatcatac ggagttccaa accgatacgc
    11041 cctttcaatt cgcgcgccaa aggcgaaccc gccttcagca tacggaatgc gcgccggcac
    11101 tcatacgcca tcagccgctg aaacgccgca tccgccctac cctccgcaat ctgtccttcg
    11161 gaaacaccga atttcaacaa atcgtcctgc gggatataaa ccctgccctt ttgccaatcc
    11221 acagccacat cctgccaaaa attcaccagt tgcaaagccg tacagatacc gtcgctttgc
    11281 gccacgcaca ccgcatccgt tttcccgtac aaagccagca taatgcgtcc gacagggttg
    11341 gcggaacgcc gacaataatc ggccagctcg ccgaaatttc cataccttgt tttaaccaca
    11401 tcctgagaaa atgcagaaag caaatcataa aacggctgca aatccaaacc gaacggcaca
    11461 accgcctcgg catccaatcg tgcaatcaaa ggatgcgccg accggccgcc cgatgccaac
    11521 acgtccaact cgcgctgcaa accctccaac cccgccaacc tggcttcaga cggcatactg
    11581 ccctcgtccg ccatatcgtc cgccgtccgt gcaaacgcgt acaccgcgtg aaccggcttc
    11641 ctcaacctgc gcggcaaaat cagcgaaccg acgggaaaat tctcataatg cccaaccgac
    11701 ataccttctc catccatcaa acaaaatgcc gtctgaaacg gaacaaaccc ttttcagacg
    11761 gcatcagata cctccaagct gccggcaatc agtggtggtg atgaccgtgc gggccgtgga
    11821 catgaccgtg tgcgatttcc tcatcggatg catcgcgcac gctttcaact gtagccttaa
    11881 agcggatttt catgcctgcc aaaggatggt tgccgtccac caccgccttg ccgtcggcaa
    11941 catcggttac acgatagacg acaacatcgc cagtttcagg atcgtcggct tcaaacatca
    12001 tgccgacttc gacttcaaca gggaacacgc ccgcatcttc gatacggacc aactccggat
    12061 cctgctcgcc gaacgcatcg tcgggcgaca gcgccacatc gaccgtatcg ccggcatcct
    12121 taccgtgcaa cgcctcttcc accaaaggga aaatgccgtc gtaaccgccg tgcagatacg
    12181 caatcggttc ttcggttttg tccaaaagct gattgttggc atcatacatc tcataatgca
    12241 gcgaaaccac ggaatttttc acgatagcca tatttgtcct ttcaggaaca gcagattaat
    12301 tacaggcgca ttctaacaca accgccgcgc cggccgatta ccgttaacct gttcataaac
    12361 tgtacagcac atatttcaat gtaaatcttt gttattttat tgcggtgtaa cttttttaca
    12421 acattcttaa aaccattccg acctgtctgc cgactttccc aatccgcctt aataaatcat
    12481 acaagatact gaaattatat taatctctat aatatttatc cctatcgaat ttttaacagc
    12541 aaaaccgttt tacaggattt atcaatccgc ccgccagaaa acttttcatt caaacctttt
    12601 tcccatctgt acgacattgc aatcccttat tccatagtgc ataattacgc aaattcagcg
    12661 atgaatttcc aacccggttt gtagtatggt cgataaagac ctatttgttt caataattta
    12721 aattggttct aaaggttact aaaatgaaaa aatccctgtt tgccgctgct ttgttgtctt
    12781 tggttctggc agcctgcggc ggtgaaaaag ccgctgaagc tcccgctgct gaagcacctg
    12841 ccgccgaagc tcccgctact gaagcacctg ccgccgaagc tcccgctact gaagcacctg
    12901 ccgccgaagc tcccgctgct gaagcacctg ccgccgaagc tcctgctact gaagcacctg
    12961 ccgctgaagc tgccgctacc gaagcacctg ccgctgaagc tcctgctgcc gaagctgcaa
    13021 aataagcatt ttccgcttgc aaaaaagcag gatacgttca gtatcctgct tttttgattt
    13081 ttcagacggc atcagattcc cttcctcaat cttctcccta cccttccgac aaacatgcct
    13141 gaccttcata ccgaattttc ccgactcctg ccggcagatg aaattgccga actttctccg
    13201 acgcttttaa aagaccagcg caaccgcttt acgtctgcac cagacatcat tttgcagccg
    13261 cgcagcgttg aaagcgtgca aaccattatg cgtttttgtt acgaacaccg cattccggtt
    13321 acgccgcaag gcggcaatac cggtttgtgc ggcgcggcag tatcggaaaa cggcgtattg
    13381 ctgaaccttt ccaaactcaa ccgcatccgc agcatcaatt tgtcagacaa ctgcataacc
    13441 gtcgaagcag gttccgtact ccaaaccgtc caacaggcag ccgaagcctc aaacaggctg
    13501 ttcccactca gtctcgccag cgaaggctcc tgtcaaatcg gcggcaacat cgcctgcaat
    13561 gccggaggtt tgaacgtatt gcgttacggc acgatgcgcg acctggttat cggtttggaa
    13621 gtcgtcctcc ccaacggcga actggtttcc catctccatc ccctgcataa aaacaccacc
    13681 ggctacgacc tgcgccatct gtttatcggc agcgaaggca cactgggcat catcactgcc
    13741 gccacgctca agctttttgc ccgtccctcg gacaaggcaa cggcatgggt cggcattccc
    13801 gacatcgaat ccgccgtccg cctgctgacc gaaacccaag ctcactttgc cgaacgccta
    13861 tgcagttttg agctgatcgg ccgttttgcc gccgagttgt cttccgaatt cagcaaactc
    13921 cccctgccga cacattcaga atggcatatt ttacttgagt tgaccgactc attacccgac
    13981 agcaatcttg atgatcggct tgtcgaattt ctttataaaa aaggatttac cgacagcgta
    14041 ttggcacaaa gcgaacaaga acgtatccat atgtgggcat tgcgcgaaaa catctccgca
    14101 tcgcaacgca aactgggcac cagcatcaaa cacgatattg ccattcctat cggacgcgtt
    14161 gccgactttg tccgccggtg cgccaaagat ttggaacaga attttaaagg catacaaatc
    14221 gtctgcttcg gacatcttgg cgacggcagc ctgcactaca atactttcct gcccgaaatc
    14281 ctcagcaatg aagtctatcg ttacgaaaac gacatcaacg acgcagttta tcgcaacgtt
    14341 cttgcctgca acggcacgat tgccgccgaa cacggcatag gtatcatcaa aaaacagtgg
    14401 ctggacaaag tacgcacgcc tgccgaaatc gccctgatga aaagcatcaa acaacacctt
    14461 gatccatata acattatgaa tccgggcaaa ctgcttccgt aaataactga ttcaaaatga
    14521 aattatagga aatatcatgg gaatgacagc aacctatcaa ctggtcgata atgaaaaact
    14581 ggctttgctt aaaaacaccg ataccgaatg gcaagaattg tttgaagaac tggaagattt
    14641 ggaggatgag gctgccgtct gcctcgacat cgataaaatg tgggatgttt tgcattttgt
    14701 actgaccgga acaagtgcag aaaatccctt aaagggcaat ctgttatccg aatcaatcgt
    14761 cggaacggca cccatttcgg acgacgacgg atacgccgct tatatcgaat ccgcccaaat
    14821 ccatgaaatc atctcagctt tggaaggctt cgacataaaa agcgcaatgg agcatttcag
    14881 tatgcaggtg tgcaaagagc atcatatcta ccccgacatt tgggattatg aagatgaaat
    14941 ttatgatata aaagaagaaa ttaccgacta cttttacaaa atcggcaatt tttacaaaac
    15001 cgcccggcat tcaaatagca atgtactggt cagcatttac taaatatatt tcaaacatca
    15061 cggaaacttt aatgaagaca aagtacggaa tcgcactcaa ggaaacctag gtatttcaac
    15121 ctgggagacc atcatgagta aaagttacag aaaacacccc ttttccccta taaccacggc
    15181 agtcagcgaa aaacaggaca aacgccttgt caaccggaaa ctacggagaa tagcgcgcga
    15241 atgtctgaaa caaggaaaag aaccacccca ccatatcagg gcagtcagca acgtttacga
    15301 tttcgccaaa gacgggcatt tccgtttttc cgcttcccgt catccgcatc ttttacgcaa
    15361 atagaaaaca gggcaaccgc cctgtttttc cgtttcgtat ttcaacacca ttgttcaaac
    15421 aaccgtcaga cggcattgtt caaacgccgt ctgaaaccgc atccccccca tcagcgcgta
    15481 tgcttaatca accgctgttt ctcgcgtttc caatccgcct ctttcatact ctgacgtttg
    15541 tcgtgctgtt tcttaccttt tgccaaaccg atttccatct tgatttttcc gcgcgaaaaa
    15601 tgcaaatcca gcggcacgat ggtgtagccg gcacgttcgg ttttgccgat taatttgttg
    15661 atttccgact ggttcaacaa gagcttgcgc ggacgtacgg catctggttt aatgtgtgtc
    15721 gaggctgtgg gcaaagccgt aatatggcag ccgaccagat aaaacgcgtc ttttttccaa
    15781 tagatataac tctctttaag ctgtacgcgc gcggcgcgga ttgctttgac ttcccagcct
    15841 tccaagacca aaccggcttc aatccggtct tcaatgaaaa aatcgtgaaa tgctttttta
    15901 ttgttcgcaa tagccataaa catcctatca atatccgccg tcagacggca taaacccgaa
    15961 aacagaaccc atcataccgc ctcttcaacc gcctgcacaa tcttctcggg atacagcctg
    16021 ttgaggcagt cggtatgccc cagcggacat tcccgcttaa aacagggcga acattccaag
    16081 tgcaggctga cgattttcgc cctgtcgctc aaaggcggcg tatgcgtcgg gctggaagaa
    16141 ccgtaaaccg ccaccacctt cctgcccaat gccgccgcca agtgcatcag cccgctgtcg
    16201 ttgcacacga ccgtgtccgc caacgacaac aaatccattg cctgcgacaa atcggttttg
    16261 ccgcacaaat tgacacacat accgtctgaa aggcagttga tttcctcggc gatttcatca
    16321 tctttttgcg aaccgaacag ccaaacctgc caacccgtcg ccgaataatg tttgcccaac
    16381 tcggcaaaat gctttgccgg ccaacgcttt gccggcccga attccgcacc cggacaaaaa
    16441 gccagaacag gctttccaag attcaaaccg aaagtttcga cagaaatttc ccgccgccgt
    16501 tcatcaatgg aaaactcggg gaatcccgaa tgcccgtcaa aatcttcctg actcggatgc
    16561 gcgagagccg tatatctgtc caccatcaaa ggcagacgtt ccttatccag cctgcgtata
    16621 tcgttcaata gaaaataacg gctttcgccg acataacccg ttctttttcc gatgcccgtt
    16681 gccagcgcga tgatggccga tttcaaagaa ccgggcagca cgataacctg atcgtagccg
    16741 cgccgtccca aatccctacc gacccgccaa cggcgtttta actccaacgc accatgtccg
    16801 aacggatttt caagaatctc attcacttca ggcatacgct caaacaccgc catcgaccac
    16861 ttcggcgcga acacatcaat cgtgcaaccg gggtgaagtt ccttcaaacg gcggaacaag
    16921 ggctgggtca tcacgcagtc gcctatccaa ctgggagaaa taatcaggat tttgatggac
    16981 ataacaagaa accgaaatca gacaggcgga attttaccgc gaaaccgttg gaaaacctat
    17041 ctcgccgcat tccgaacgcc ggacgtgcaa atatgaaaaa gcccgaacat tcaagttcgg
    17101 gcttcaaaat tctggctccc cgacctgggc tcgaaccagg gacctgcgga ttaacagtcc
    17161 gtcgctctac cgactgagct atcggggaat ggggcgtatt atagcgtccg gaaaaaatgt
    17221 gtcaatcctt aattttggaa aaatgggcga caaaacgaca agcatatgaa tcagaaagac
    17281 attaagaccg atgccttaaa aggattgccg ttgtatgaat ttccacagcc gtcatcacac
    17341 catatttaag cccgatgagc cgttctgccc tcccccgctt aaaacaatgc cgtctgaact
    17401 tcgccgcgtt ccaaagccag taaaaactgt ttgcggttca acccgcccgc gtagccggtc
    17461 agtttgccgt cgctgccgat gacgcggtgg cagggaatca ggacagacac cttgttctgc
    17521 ccgttggcgg cggcaacggc gcggacggct ttggggttgc ccaaacgctg cgcctgctcc
    17581 ttgtagctgc gcgtttcgcc gtaaggaatc gccaagagcg cgtcccatgc ctgcttttga
    17641 aactcggtgc caatctgctc caaaggcgtg gcaaaggttt tcagacgacc cttgaagtat
    17701 aagtccaatt cctgccgcaa aagttgcgtc cgctcatcct cccgaaacac aaaccgtccg
    17761 cgcaaggctt tttggacggc ggcaatttcc tgttccaaat gcttctgtcc gacaaactcc
    17821 agcagacaca atcccttatt tccgaacacc gccagcatct cgcccaaagg cgtggcaatg
    17881 gcggcacaca ccagctcatt caaactgtcg ggataacgcg cttccaacag acggatggct
    17941 cggcggatac ggacatattc ttcaggcgcg cagccgatat tgtcccaaaa atcccgatcg
    18001 aactgcccgg cttcgcattc cgtcaaattg ggatgcggca taacgccgca ctcacaaacc
    18061 cgggtttcga gccaatagcg gatttcatcc cattttgacg gcagattgtt taaggaaggc
    18121 agggtaatca ttcgtttgct ccgtatctct atcatggatt tgacggcaaa attcccaatt
    18181 ttcgccattc ccgcacgctg atgcaggaac gggctacgac gtaaatcttg agggttaggt
    18241 tgcggcaatg cctaaatatg cgatatttcc aaagcctcag ggaaaggaat taaagacttc
    18301 agacgacctg caaaggctcg tctgaaacca cgatttttgc atttgcgcat tctggcacat
    18361 catccaaccg tttcggcaca ttcctgccgc cgttgacagc ctataatgaa tccacttatt
    18421 catcaagcaa aggaatcatc tatgcaaacc cgcattctct ccgccgtact gctggctttt
    18481 tcaaccgctg cctttgccgg gggcgcattc acgctgcaat tcgacaaccc gtccgaagac
    18541 ggcggcttca cgcaaaacca gcttttgagc gcgccttacg gatttggctg ttcgggcggc
    18601 aatgcttcgc ccgcgctgtc gtggaaaaat ccgcccgccg ggacaaaaag tttcgtcctg
    18661 accgtttacg ataaagacgc gccgaccgga ctgggctgga tgcaccgggt ggtcgccgac
    18721 attcccgccg atgtccaccg ccgcaacgcg acctcgctgc aattaagccg ctgcgccaac
    18781 atcgccgacg accagtccgc agccatatcg gcggtaatca gtttgcggat ttgccgcatc
    18841 aggttgacgc cttcgtacac ggcaaaaccg atgccgtcat gctgcaacca cgccaacacg
    18901 ccgcaaagcg cgacctccgc cgcattgtgc ggcacttctt catccgtcag caccgccgcc
    18961 gcataatcaa acgccgcgcc catccgcccg gggtgcggca gcttcaccgc atcgcacact
    19021 gcctgcgccg tcccgtattg tgcggcaaac ctttctatgg cttcctgttc gaaagcaatc
    19081 cattgcgcct gatagaggcc gtctgaatcg ggaatattaa taacgtcaaa cgtctgcccg
    19141 cctgccaagg cgaccgcctc acccgccgca atcctcaatt cgcgcctcgc ccaccgcagc
    19201 ttcttgcttc cgcgccgcac gataagcaca gccggttcat ataccgccac gctgcggtac
    19261 aaggcggtat gatgttgcac gatgccgccc aaagcaccca atcgttcgcg cgtatgaaag
    19321 taatttgacg gcactattat ctccaacacg cagggccgca cataaggcgc agccctgttt
    19381 accctgattt ggaaagggtt acttcccaaa aaataaacca aaatcccacc gccccgaatg
    19441 gccgggtttc aaccatattc cgtcggcgtg cctgaaactt gacccctccc ctgtcgggga
    19501 gggtcgggga gagggtaaaa cggggcagat acaggcaata tttccgttgc caccccgatg
    19561 ccctctccct aaccctctcc cacgggagag ggaatggatt gccgttgaaa taaatcgctc
    19621 tacataaaaa atcaatgtgt tatctcaaac ccacattagg tctaatcaaa tggtcggata
    19681 tccatattcg gcaagcaagc tgctttcaga cggcatttcc agccaacaag cgcgccaata
    19741 tcccctcata caccgcagac agcttcggaa tgtcgtttag ccgcacgttt tcgttgattt
    19801 ggtggatggt ggcattggac gggcctaatt cgatgagttc ttttgcaatg gctttgatga
    19861 agcgtccgtc cgaagtgccg ccggtggtgg acaattcggc ctcaatgccg caggtttcgg
    19921 caatggctgc gcgtgccacg tcggtcagtt tgcccgcttg ggtcagaaag ggctgccccg
    19981 aacacgacca ctgtaaatcg tattgcacgc cgtgtttgtc caaaatggcg tgaacgcgtt
    20041 gtttcagccc tgcttcggtg gactcggtgg agaagcggaa attgaatttg acattcagct
    20101 cgcccggaat gacgttggtc gcgcctgtac cgccgttgat attggaaatt tgaaagctgg
    20161 tcggcgggaa gtatttgttg ccttcgtccc agacttcctg cgtcagctct aacaaggccg
    20221 gggcaaaagt atgcacggga ttgattgcca aatgcggata ggcaatatgg ccttgcttgc
    20281 ctttgacggt caggttgccc gacagcgagc cgcgccggcc gtttttaatc atatcgccca
    20341 atttgtccac ggctgtcggt tcgccgacga tgcagtagtc aatcaactcg ccgcgcgctt
    20401 tcaatacatc gacgactttg gtcgtgccgt ccaacgcgtc gccctcttcg tcggaagtaa
    20461 tcagaagcgc aatgctgcct tggtggtcgg gatgttcggc aacgaagcgt tcacaggcgg
    20521 taacgaagca ggcgatgctg gttttcatgt ccgccgcgcc gcgcccgtat aatcttccgt
    20581 cgcgttcggt cggctcgaac gggggcgaat cccatttttc aacagggcct gtcggtacaa
    20641 cgtcggtatg ccctgcaaaa cagacgacgg gaactttcgt gccgcgtcgc aaccagatgt
    20701 ttttggtgtc gccgaaatgg agttcttcag ccgcaaaacc gattttgtac aggcgttcga
    20761 caagcagttt ttggcaatct cggtcgtcgg gggtaacgga tgggcgggaa atcaacgctt
    20821 tggctagttc tagggattgg gtttcggtca tatttgttca cttttgaaat tagaccatct
    20881 gaaacgttct gaatgtgatt ttcagacggc atttaggtta ggttggcata cggggtgggt
    20941 atttttaccc gtcagtcttc tgaatcattt gccgtgacac gcttcgtaaa gaggcagcaa
    21001 atcttccacc gtttccgcta tccatttcgc gacatcctgc ctgcccaaat cgtcgcgttc
    21061 gatgtgtttg ccgatgcaga aaaagtcttc gtcgttttgc aactttcggt cggactcgtt
    21121 ttgttgcgcg acggtgcggt agtcgtcgta ttcgctttcc gcaccgtgcc acatatcgaa
    21181 agaagcgtat ttttcggtat caaaattatc caaccagcgg ttgtaatcag gcagcgcgat
    21241 gggcgacacg tcggctttgt agcagtgcca atccaagctg acgctcagtc ggcggcggtt
    21301 gagcagtatc gacaaaatcg cggcggaatt tttatattgt tcgtatttga agtaggcaaa
    21361 gaaatgggcg cgaacctgcc agccgttaca ccagcgttcg atgtgcggcg gcgcaaacgg
    21421 cgcacccaat tcggcggcaa cctgctgaat cagctgctgc catatctgcc agttttcttt
    21481 atagtcagcc ttgatttgcg gaatgctttc aggctggtat tttttaagct gggaaaattg
    21541 gaaaaacggg atattgaaca aatcgcaact tttcggggtc agcataatat atccttgaga
    21601 cgattgtttc agacggcatt atttgcgccg gcgcgccgcc ataatttcgc cgatttcggt
    21661 cagtttttct tttgggataa aggtgttgcc catatcaaac agcggctctt caatcgccaa
    21721 atgaacatca tatcccgcca caaaacgttt gaacgcttcc tcatcgggga cataagcgtt
    21781 gtctgcttcg agtttggcaa attcggcgga aacagccgcc cagttgtcgt gcagcccgat
    21841 atgttggcgc aaaagctcgt ccacgctttc ttgggcttgc ggcgcatatt gcagcagcag
    21901 cgggaagaag ttttcttctt cgtcttcatg gtgcagcggc gcggcaacgt tgaaatactg
    21961 ggcgatttgg cggatggttt gcaaaacaag ctgattgcag ccgttttcgg cgatgtagtc
    22021 cgacagcatc gcgacttggc cgcaaaaacg gcgtactttg ccgtggcagg catacagcat
    22081 ttcaatcggt tcggcaaagg taacgctttg ggtttcaaac agattcatgt tttcgttctc
    22141 aacggggcac ttttcaagca gtcattttat aataaaacag cctgcacaaa gcaggctgtc
    22201 cgtcttttga gactttaagc ggattaatcg accaaagtca ctttgccgtt catcaaagca
    22261 ccgtgacccg ggaaggtgca ggcaaatttg tattcgccgt cagccagttt ggcaggatcc
    22321 agagtcaggg aagcttcttc gccgccgccg atcagtttgg tatgggcaac aacgcgtgca
    22381 tcatcaggtt taacataatc ggtatcggca gcacctacgc cgtctttaaa tacgccgtcc
    22441 atatcttcag ctttggcaat cacgagattg tgacccatgc tggttttggg ttgcgtaccg
    22501 gtatgtttca gagtgatggt gaactcttta catgctttgc tgacttggat gtctttggtg
    22561 ttgaactgca tattgtcgtt ggattcgaca gttgccgcac agttgccggc agcaggggct
    22621 tcggcagcat ctgcaggagc agcttcggcg gcaggcgctt cggaagcggg tgcttcagca
    22681 gcaggagttg cctcggcagc aggcgcggca ggttcttgag agcaggcagc caaaccgata
    22741 acggcggcag aaatcagagc cagatacgct ttcataacaa atctccaatc gataaaataa
    22801 tattcggttt tacagaaatc aaagtgcaac cgccattaac aaaaccttga aaaagattcc
    22861 gccgcgttgc acaaacagat gtttcggagc ggcattttgc tacaaatttc atttgaaatc
    22921 aaagcctgtc tgcaagttta caatcgttta ctttaaaagg ggcaatttta ccccgaacct
    22981 atttctttag tattagaccc attatccttt acttcttaat attaacggat gtttacacaa
    23041 attcccgtat acattttatg cgccatgcct tctaaccaag tttgccaatg cctccgccaa
    23101 ttcgggatgc cgtttttcca actttaccgc cgccgaaccg aaactctcca gcgcagcctt
    23161 actcaaatgc agggtattgg ttttcggcgg tttttccggt ttcgggacca gcctgaccga
    23221 aacagagcgt atcgaagcgt caagccctgc caactgcggc aataccgacg gtgcaatcat
    23281 tttcaagcgc gatgccgcca tattgtttgc cgccaaaagg acaagcctgc cgtcttcgat
    23341 acatgccgtc tgaaaatgcg ggtgcaggtt ggcaggcagc agttttttca cggcggcatc
    23401 caaccgccgc cactgtcccg cctgtttcaa aagtccggaa agcagcgcgt cccgcctgcc
    23461 caactgttcc aaattcataa aacatacacc caaaaagatt gaaatacctc aaacgcgcct
    23521 ttatttcaga cggcattagc actttgcaca aacgcttgtg ttaaaatcgc gttttcgccc
    23581 actattatat caggcgcagg aattattcat gctgacaaac attgccaaga aaatcttcgg
    23641 cagccgcaac gaccgcttgc tgaaacaata ccgtaaatcc gttgccagaa tcaacgcgct
    23701 cgaagaacag atgcaagccc taagcgatgc tgatctgcaa gccaaaactg ccgaattcaa
    23761 acaacgcctc gccgacggtc agactttgga cggcattttg cccgaagcct tcgccgtctg
    23821 ccgcgaagcg tcccgccgca ccctcggtat gcgccacttc gacgtgcagc ttatcggcgg
    23881 tatggtgctg cacgacggca aaatcgccga aatgcgtacc ggcgaaggca aaaccctggt
    23941 cgccaccctc gccgtctatc tcaacgcgct ggccggcaaa ggcgtacacg tcgttaccgt
    24001 caacgactac ctcgcctcac gcgatgcggg cattatggag ccgctctata atttcctcgg
    24061 tctgaccgtc ggcgtgatta tttcagatat gcagccgttc gaccgtcaaa acgcctatgc
    24121 cgccgatatc acctacggca ccaataatga attcggcttc gactacctgc gcgacaatat
    24181 ggttaccgac caatacgaca aagtgcagcg cgaattgaat tttgccgttg tcgatgaagt
    24241 ggattccatc ttgattgacg aagcgcgcac tccgctgatt atctccggtc aggcggatga
    24301 caacatccag ttgtaccaaa tcatgaacac cgttccgccc cacctcgtcc gtcaagagac
    24361 agaagaaggc gaaggcgact attgggtcga cgaaaaggca catcaggtca tcctgagcga
    24421 agcaggtcac gaacacgccg agcaaatcct gacccaaatg ggattgctgg cagaaaacga
    24481 ctccctctat tccgccgcca atatcgccct gatgcaccac cttatggcgg cattgcgcgc
    24541 gcattccctc ttccacaaag accaacatta cgtcatccaa gacggcgaaa tcgtcatcgt
    24601 ggacgaattc accggccggc tgatgtccgg ccgccgctgg tcggagggtc tgcatcaagc
    24661 cgtcgaagcc aaagaaggcg tggaaatcaa acgcgaaaac caaacccttg cctccatcac
    24721 cttccaaaac tatttccgcc tgtacaccaa gctctccggc atgaccggca cagccgatac
    24781 cgaagctttc gagttccaaa gcatctacaa cctcgaaacc gtcatcatcc cgaccaaccg
    24841 ccctgtacag cgcaaagact tcaacgacca gattttccgt tccgccgaag aaaaattcga
    24901 agccgtcgtc aaagacatcg aagaatgcca caaacgtggg cagcccgtcc tcgtcggcac
    24961 caccagcatc gaaaactccg aactggtctc ccaccttctg caaaaagccg gactgccgca
    25021 caacgtcctc aacgccaaag aacacgaacg cgaagccctg attgtcgccc aagccggcaa
    25081 agtcggcgcg attaccgttg ccaccaatat ggcgggacgc ggtacggaca tcgttttagg
    25141 cggcaacctg aagcaccaaa ccgatgccat ccgtgccgac gaaaccttga gcgacgaaga
    25201 gaaacaggca caaatcgccg cactcgaaaa cggctggcag gcagaacacg ataaagtaat
    25261 ggaagcaggc ggcctgcaca tcatcggtac ggaacgccac gaaagccgcc gtatcgacaa
    25321 ccaattgcgc ggacgttccg gccgtcaggg cgaccccgga tccagccgtt tctacctttc
    25381 gtttgaagac ccgctgctgc gcttattcgc actcgaccgc gccgccgcca tcctcaaccg
    25441 tctcgccccc gaacgcggcg ttgccatcga acacaacctg ctgacgcgcc aaatcgaagg
    25501 ggcgcaacgc aaagtcgaag gcagaaactt cgatatgcgc aaacaggttt tggaatacga
    25561 cgacgttgcc aacgaacagc gcaaagtcat ttacagccag cgcaacgaaa ttctgaccag
    25621 caaagacatc agcgacctga tgcaggaaat ccgttctgat gtcgtcagcg acctcgtgga
    25681 tacctatatg cctcccgaca gcatggaaga acaatgggac atcccgactt tggagaaccg
    25741 tctggctgcc gaattcagac tgcacgaaga catccaatcc tggctgaagg cggacaatgc
    25801 gattgacggt caagacatca aagaacgcct gatcgaacgc atcgaaaacg aatatgccgc
    25861 caaaaccgaa ctggtcggca agcaggcaat ggccgatttc gagcgcaacg tgatgttgca
    25921 ggtcatcgac aaccaatggc gcgaacacct cgccgctatg gactacctgc gacaaggcat
    25981 acacctgcgc agctatgccc aaaaaaatcc gaagcaggaa tataaacgtg aagcctttac
    26041 catgttccaa gacctgtgga acggcatcaa attccatatt gcctccctgc ttacctcggt
    26101 tcaaatcgaa caaaaccctg tcgcggtggt tgaagagcaa cccatcggca acatccaatc
    26161 catccattcc gaatcgcccg atatggaaga acttttgggt cagtcgcaaa ccgatctggt
    26221 taccgaagcc tttaatcccg atggaacgga tttcagcccc gaagccttgg aagcgcgggg
    26281 acaaatcgtc caccgcaacg acccctgccc ctgcggcagc ggtttgaaat acaaacaatg
    26341 ccacggcaaa ctggcttgag cgtttgaacg caaatgccgt ctgaacatcc ggtttccggc
    26401 ttcagacggc attttgcctg aaccgccaca tccgactgcc attccgaaaa atcccgattt
    26461 cgtaccgtcc aaactaaaaa caggcatccg ccccgccgaa ctatgattcc atccgacttc
    26521 attgacgaac ttctagccaa aaccgatatt gtcgacatca tcgacgaaca ggttccgctg
    26581 aaaaaaggcg gggcgaacta tatggcgtgt tgcccgttcc acaaggaaaa aacaccgtcg
    26641 ttttcggtca gtccgaccaa gcagttttac cactgtttca gttgcggggc gcacggttcg
    26701 gcgattggtt ttgtgatgga gcatcagggg ctgtcgtttc cggaggcggt tcagttcctt
    26761 gccgaccgcg tgggtatgat tgtgcctaaa gtgcgcgggc agaacgataa tcccgaagtc
    26821 cgtgccgaac gtaagaaaaa gcagcagacg ctggaagaaa cgacggcggc ggcggctgat
    26881 ttttatgcgc aacagttgcg cttcaatccg acggcgaagg cttatttgga caagcgcggc
    26941 ttgagcgcgg aagtcatcgc gcattatggt ttgggctacg cgcccgacgg ctggcagcct
    27001 ttggcgcaag tattccaacc gtacccgaat accgcgttgg tggatacggg tatggtgatt
    27061 gacaatgagg ggcggtatta cgaccgattc cgtcatcgga ttatgttccc catccgcaat
    27121 ccgcgcgggc aggtcatcgg tttcggcggc agggtgctgg acgactccaa acccaaatat
    27181 ctaaactctc ccgatacgcc tttgtttgat aaggggaaaa acctttacgg attgtatgaa
    27241 gggcgtgctg ctgtcaagga agtggggcgg attctggtgg tcgaaggcta tatggacgtg
    27301 gtcgcgctgg cacagttcgg cgtaggctac ggtgtggcgg ccttgggcac ggcaacaaca
    27361 gcggaacacg tcaaaatcct gatgcgtcag gcggacagta tttatttctg tttcgacggc
    27421 gacagcgcgg ggcgcaaagc ggcttggcgc gcgctggaaa acgcgctgcc gcaattgaag
    27481 gacgacaaat cgctgcattt tttgttcctg cccgaggaac acgaccccga cagctacatc
    27541 cgcgcctacg gcaaagcgca atttgaagat gcgctgttga atcaaagcaa gcccttgtcg
    27601 gaatattttt gggaacatct tacagacggc attcatctca atacgcagga aggcaaggcg
    27661 gaattggtaa aaaccagttc gccgcttttg gcgcagatta ccgcgccggc attggcttat
    27721 ttgttaaaac aacggcttag cgagctggtc ggcatcgacc ccgacaacct cgcgcaactg
    27781 ctcggacagg aagcgccaaa gcggcacgtc aaacaaaaaa actacaaact gcccccgatt
    27841 tccgtcaaac agcccgttat gccgacattg gtgcaacggc aaatccgcag cctcttgata
    27901 aatccggatt gggctgcata tatagacctg cccgattatt tggcgttgga cggcgatttc
    27961 gcctgccttg ccaacctcgc cgaaaccatt aaaaaccatc cttccgtgcc ggcaaccgca
    28021 caggttttgg aacatatgcg cggttcgccc tacgaagaaa caatcaaccg catcttccgg
    28081 tcggcccttc aatcggaaga aatggaaggc ggcggcgaag aagattgcga aaacttccaa
    28141 atcggcatca aaaaactgct caatgagtta aaatacagcc aaatcgaaac attaaaacaa
    28201 aaaagcctgc aatccggctt aaatgaaagc gagaaaaaac ttttgctgtc gctgctgacc
    28261 gcaaaacaaa attgaccggc ggattccgcc atccgtaaac cgttatgccg tctgaaaagc
    28321 attcaccccg gctgcaacaa cgacacctgc agaacaccca tcccaaaaag ccttcagacg
    28381 gcatcagagt accctactct gccacgcctt caggtgcgtc caaacgcaaa ccgtcggcat
    28441 cttgccaaca gaaagcagac aatgtccaaa aaccaaaatc acgaagaata ccaagacgat
    28501 gcccgccccc tgacgattga ggaacaacgc gcgcgcctgc gccaactcat cattatgggc
    28561 aaagaacgcg gctacatcac ctactccgaa atcaacgacg cactgcccga cgatatgtcc
    28621 gatgccgatc aaatcgacaa catcgtcagt atgatttccg gtttgggcat ccaagttacc
    28681 gaacacgccc ccgatgcgga agacatattg ttaagcgaca atgccgccgt taccgacgat
    28741 gatgccgtcg aagaagccga ggccgccctt tccagcgcag attccgagtt cggcaggacg
    28801 accgaccccg tccgcatgta tatgcgcgaa atgggacagg tcgacctgct gacccgcgaa
    28861 gacgaaatca tcatcgccaa aaaaatcgaa aacgccctga aaaatatggt tcaggccatc
    28921 tccgcctgcc cgggatccat tgctgaaatc ttagaactca tcgaaaaaat ccgcaaagac
    28981 gaaatccgcg tcgacgaagt cgtagaagcc attatcgacc cgaatgaagt attgctcaac
    29041 gaattgggct tggggcactt ggaaaccaca gcacccgaga aaccttccaa cgacaattcg
    29101 gacgaaaacg aagacgacga agaatcggaa gaagatgcgg atgaaatctc ggcagccaat
    29161 ctcgccgaat tgaaacaaaa agtcatcggc cactttgccc aaatcgaaaa agactacaaa
    29221 aaaatgatcg gccgtttgga aaaacaccac agccggcaca aagactatct cgcctaccgc
    29281 gacgcgattg ccaacaaact gctggaagtc cgtttcgcca cccggcaaat cgacagcctc
    29341 agcagcagcc tgcgcgggaa agtagaaaac atccgcaaac tcgaacgcga aatccgcgac
    29401 atctgcctcg accgcgtcca tatggaacgc gactacttca tccaaaactt cctgcccgaa
    29461 atcaccaatc tggaatggat tgaagaagaa atcgccaaag gcagggtttg gagcgacgcg
    29521 ctcgaccgct tccgccacgc catcctcgaa aaacaaaccg agttggcgga tatggaaaaa
    29581 gaaacccgca tttccatcga agagttgaaa gaaatcaaca aaaatatggt gtcgagcgaa
    29641 aaagaaaccg cagccgccaa acaggaaatg attcaggcaa acttgcgcct cgtcatttcc
    29701 attgccaaaa aatataccaa ccggggctta caattccttg atctgattca ggaaggcaac
    29761 atcggtttga tgaaagcggt cgataagttc gaataccgca gaggctataa attctccacc
    29821 tacgcaacct ggtggatccg ccaggcaatt acacgctcga ttgccgatca ggcgcgtacc
    29881 atccgcattc cggtacatat gattgaaacc atcaacaaga tgaaccgcat ctcgcgccaa
    29941 caccttcaag aaaccggcga agaacccgat tccgccaaac ttgccgaact gatgcagatg
    30001 cccgaagaca aaatccgcaa aatcatgaaa atcgccaaag agccgatttc gatggaaacc
    30061 cccatcggcg acgacgacga ttcgcacttg ggcgacttca tcgaagatgc caacaatgtt
    30121 gcgccggccg atgcggcaat gtacaccagc ctgcacgaag taaccaaaga aatactcgaa
    30181 agcctgaccc cgcgtgaggc aaaagtcctg cgtatgcgtt tcggcatcga tatgaacacc
    30241 gaccacacgc tggaagaagt cggcagacag tttgacgtaa cgcgcgaacg catccgacaa
    30301 atcgaggcaa aagcactccg caagctgcgg catccgacaa gaagcgaccg tttgagaagt
    30361 ttcttggaca gcgaagacag caagctgtaa accaaaaaac cgcaggtttc aaatacctgc
    30421 ggttttttct tacacaataa acaacgcttc cacatatccc acactcctat cccgagacct
    30481 ttgcaaaatc ccccaaaatc ccctaaattc ccaccaagac atttaggagc accttcttcc
    30541 aacaaaccgc gcaagccatg attgccaaac acatcgaccg cttcccacta ttgaagttgg
    30601 accaagtgat tgattggcag ccgatcgaac aatacctgaa ccgtcaaaga atccgttacg
    30661 tccgagacca ccgcggtcgt cccgcctatc ccctgttgtc catgttcaaa gccgtcctgc
    30721 tcggacaatg gcacagtctc tccgatcccg aactcgaaca cagcctcatt acccgcatcg
    30781 atttcaacct gttttgccgt ttcgacgaac tgagcatccc cgattacagc accttatgcc
    30841 gctaccgcaa ctggctggcg caagacgaca ccctgtccga attgctcaaa ctgattaacc
    30901 gccaactgac cgaaaaaggc ctaaaaatag agaaagcatc cgccgccgtc attgacgcca
    30961 ccattattca gaccgccggc agcaaacagc gccaagccat agaagtcgat gaagaaggac
    31021 aaatcagcgg ccaaaccaca ccgagtaagg acagcgatgc ccgttggata aagaaaaacg
    31081 gcctctacag actcggttac aaacaacata cccgtaccga tgcggaaggc tatatcgaga
    31141 aactgcacat caccccagcc aatacccatg agtgcaacca cctgttgcct ttgctggaag
    31201 gcattaccga aggcacgacc atctatgccg acaaaggcta tgacagtgcg gaaaaccgaa
    31261 catctggaag aacatcagtt gttagacggc attatgcgcg ggcagcctat ttcggactga
    31321 ttaaagtgag tgcgcaaagc ca
//
LOCUS       DAWFIE010000016        29720 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWFIE010000016 DAWFIE010000000
VERSION     DAWFIE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.016"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..676
                     /locus_tag="WGQ71_000679"
     CDS             <1..676
                     /locus_tag="WGQ71_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0706732.1"
                     /translation="AHIRAGDRVLSKDEASGETGYKPVTARYGNPYRETVYIKVSDGI
                     GKIQTLVSNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITFKQQPLKAYNLT
                     VADWHTYFVKGDRAETEGVWVHNDCPYGEKYRTEVGSYTNTHESGRTYSGKGTRQRSQ
                     ISGAREARVNNDPHIATDFTPAKNNREAFKDESRRIDAHGGSKSTNNYNRIESPGKKY
                     RKQDGD"
     gene            679..1002
                     /locus_tag="WGQ71_000680"
     CDS             679..1002
                     /locus_tag="WGQ71_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ0706733.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     RLISYRQDFQISIEQNEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            1061..1288
                     /locus_tag="WGQ71_000681"
     CDS             1061..1288
                     /locus_tag="WGQ71_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706734.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            1415..1957
                     /locus_tag="WGQ71_000682"
     CDS             1415..1957
                     /locus_tag="WGQ71_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0706735.1"
                     /translation="MINHIEKYIGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LELSKHDLNYKSRFEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVIYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLDNEVDNLSDMNRKPFVW"
     gene            2122..2799
                     /locus_tag="WGQ71_000683"
     CDS             2122..2799
                     /locus_tag="WGQ71_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0706736.1"
                     /translation="MAHIQTGEYVFAKDEASGKTGYKPVTARYGNPYQETVYIEVSDG
                     IGNNQTLISNRIHPFYSGGKWIKAEDLKAGSRLLSESGKTQTVRNIVVKPTPLKAYNL
                     TVADWHTYFVKGNQAETEGVWVHNDCPYDKGNQRYKDASYHGKNDNSVKSRAPTNGQA
                     ALDNSVQVKSTSPRRVGVDKANNEIVVLDKTQTFNNGSAEYHGHVRSWQDLHTDQKNA
                     LKKAGLD"
     gene            2825..3154
                     /locus_tag="WGQ71_000684"
     CDS             2825..3154
                     /locus_tag="WGQ71_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706737.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVISLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            3571..3972
                     /locus_tag="WGQ71_000685"
     CDS             3571..3972
                     /locus_tag="WGQ71_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706738.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            4154..4543
                     /locus_tag="WGQ71_000686"
     CDS             4154..4543
                     /locus_tag="WGQ71_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706739.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLL"
     gene            complement(4584..6041)
                     /locus_tag="WGQ71_000687"
     CDS             complement(4584..6041)
                     /locus_tag="WGQ71_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ0706740.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSYIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMSWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFASHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILTAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(6056..6886)
                     /locus_tag="WGQ71_000688"
     CDS             complement(6056..6886)
                     /locus_tag="WGQ71_000688"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ0706741.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRITKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLKGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(6964..7353)
                     /locus_tag="WGQ71_000689"
     CDS             complement(6964..7353)
                     /locus_tag="WGQ71_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0706742.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAIAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(7489..8013)
                     /gene="nspA"
                     /locus_tag="WGQ71_000690"
     CDS             complement(7489..8013)
                     /gene="nspA"
                     /locus_tag="WGQ71_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ0706743.1"
                     /translation="MKKALATLIALAIPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSIGLGVLTGVSYAVTPNVDLDASYRYNYIGKVNTVKN
                     VRSGELSVGVRVKF"
     gene            complement(8184..8378)
                     /locus_tag="WGQ71_000691"
     CDS             complement(8184..8378)
                     /locus_tag="WGQ71_000691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706744.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKPYACTCNNPTDTA
                     VFSKPFASFTHPPRDAAATI"
     gene            complement(8598..9797)
                     /locus_tag="WGQ71_000692"
     CDS             complement(8598..9797)
                     /locus_tag="WGQ71_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ0706745.1"
                     /translation="MSTFPNRTMHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDF
                     NSHSLKNGLPEAAYIDALLTDLQLELPNIWGRPVETVFFGGGTPSLFQAESIDRLLSG
                     VRSLLRLQPEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNNDMLSRLGRVHN
                     GKEALTAIATALKLFDKINIDLMYALPNQTVRTALDDVQTAIATGATHISAYHLTMEP
                     NTPFGHTPPKGLPQDEAALDIEDAVHGALENAGFIHYETSAFAKPAMRCRHNLNYWQF
                     GDYLGIGAGAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEF
                     MMNALRLTDGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLN
                     DLLQCFL"
     gene            complement(9830..12355)
                     /gene="ligA"
                     /locus_tag="WGQ71_000693"
     CDS             complement(9830..12355)
                     /gene="ligA"
                     /locus_tag="WGQ71_000693"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ0706746.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARRQGGFVAEEHIQELAYF
                     QKLGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQHELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGKTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMRETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVAHSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPDFKISENKAQALWELAGKNIEGLQNDKALPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGLNEAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            complement(12503..13789)
                     /locus_tag="WGQ71_000694"
     CDS             complement(12503..13789)
                     /locus_tag="WGQ71_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ0706747.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPIPVPQTPTKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQEEMLKVGIQPGGKTALRLFS"
     gene            complement(13983..14555)
                     /gene="ampD"
                     /locus_tag="WGQ71_000695"
     CDS             complement(13983..14555)
                     /gene="ampD"
                     /locus_tag="WGQ71_000695"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ0706748.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFKYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            14639..15634
                     /gene="mltG"
                     /locus_tag="WGQ71_000696"
     CDS             14639..15634
                     /gene="mltG"
                     /locus_tag="WGQ71_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ0706749.1"
                     /translation="MLKKMLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            15694..16314
                     /locus_tag="WGQ71_000697"
     CDS             15694..16314
                     /locus_tag="WGQ71_000697"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ0706750.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQSREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNNIEKVLDGHFGC"
     gene            16536..17816
                     /locus_tag="WGQ71_000698"
     CDS             16536..17816
                     /locus_tag="WGQ71_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ0706751.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRSCVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADFEAYAAKLGEWKL"
     gene            17899..18169
                     /locus_tag="WGQ71_000699"
                     /pseudo
     CDS             17899..18169
                     /locus_tag="WGQ71_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18209..19243
                     /locus_tag="WGQ71_000700"
     CDS             18209..19243
                     /locus_tag="WGQ71_000700"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ0706752.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIAAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAASRAEDAGDEPLLLFSKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLDSVDTVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            19449..20030
                     /locus_tag="WGQ71_000701"
     CDS             19449..20030
                     /locus_tag="WGQ71_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ0706753.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            20099..20281
                     /locus_tag="WGQ71_000702"
     CDS             20099..20281
                     /locus_tag="WGQ71_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ0706754.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARTLSEEELKA"
     gene            20278..21039
                     /gene="kdsB"
                     /locus_tag="WGQ71_000703"
     CDS             20278..21039
                     /gene="kdsB"
                     /locus_tag="WGQ71_000703"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ0706755.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTVCQAHGVEVVMTSNRHESGTTRLAEAAAALKLPQHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYLRDAMRAGKREIPSETAVLRHIGIYAYRVGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            21062..21379
                     /locus_tag="WGQ71_000704"
                     /pseudo
     CDS             21062..21379
                     /locus_tag="WGQ71_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            21484..21681
                     /locus_tag="WGQ71_000705"
     CDS             21484..21681
                     /locus_tag="WGQ71_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706756.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDVAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            21838..21930
                     /locus_tag="WGQ71_000706"
     tRNA            21838..21930
                     /locus_tag="WGQ71_000706"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21872..21874,aa:Ser,seq:gct)
     gene            22233..23018
                     /locus_tag="WGQ71_000707"
     CDS             22233..23018
                     /locus_tag="WGQ71_000707"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ0706757.1"
                     /translation="MSRIRQAFAALNGEKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESV
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAVVGALVKELKDAVR"
     gene            23055..23927
                     /locus_tag="WGQ71_000708"
     CDS             23055..23927
                     /locus_tag="WGQ71_000708"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ0706758.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(23987..24598)
                     /locus_tag="WGQ71_000709"
     CDS             complement(23987..24598)
                     /locus_tag="WGQ71_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ0706759.1"
                     /translation="MRRTTLLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            24707..24931
                     /locus_tag="WGQ71_000710"
     CDS             24707..24931
                     /locus_tag="WGQ71_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ0706760.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDSSEQDGVFTLVVKHK"
     gene            24932..25078
                     /locus_tag="WGQ71_000711"
     CDS             24932..25078
                     /locus_tag="WGQ71_000711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706761.1"
                     /translation="MPSEMRMSRPTALFLNIVCAACRFKNKPSGVQCRHFGRIAADLF
                     RNGC"
     gene            25294..26328
                     /gene="pyrC"
                     /locus_tag="WGQ71_000712"
     CDS             25294..26328
                     /gene="pyrC"
                     /locus_tag="WGQ71_000712"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ0706762.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(26409..26834)
                     /gene="nusB"
                     /locus_tag="WGQ71_000713"
     CDS             complement(26409..26834)
                     /gene="nusB"
                     /locus_tag="WGQ71_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ0706763.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(26912..27388)
                     /locus_tag="WGQ71_000714"
     CDS             complement(26912..27388)
                     /locus_tag="WGQ71_000714"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ0706764.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(27435..27758)
                     /locus_tag="WGQ71_000715"
     CDS             complement(27435..27758)
                     /locus_tag="WGQ71_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ0706765.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNQPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLANIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPKD"
     gene            27925..28644
                     /gene="rnc"
                     /locus_tag="WGQ71_000716"
     CDS             27925..28644
                     /gene="rnc"
                     /locus_tag="WGQ71_000716"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ0706766.1"
                     /translation="MKDDVLKQQAHAAIQKRLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMDVGEGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCYAKGTSRKAAEQ
                     EAAKEALKWLEKKLPLKKKKK"
     gene            28691..29614
                     /gene="era"
                     /locus_tag="WGQ71_000717"
     CDS             28691..29614
                     /gene="era"
                     /locus_tag="WGQ71_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ0706767.1"
                     /translation="MDIETFLAGERAADGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDAV
                     VFVVEAMRFTDADRVVLKQLPKHTPVVLVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         7030 a         6932 c         8565 g         7193 t
ORIGIN      
        1 tgcccatatc cgagccggag accgcgtcct ttccaaggac gaggcaagcg gagaaacggg
       61 atacaaaccc gttaccgccc gatacggtaa tccgtatcgg gaaaccgttt acattaaagt
      121 ttcagacggc atcggtaaaa tacagaccct cgtttccaat aaaatccacc cgttttacag
      181 tcaaggaaaa tggatacagg caggtcgtct gaaaaaaggc gacaccctgc tttccgaaag
      241 cggcgcaaaa cagacggttc aaaacattac cttcaaacag cagccgctca aagcctacaa
      301 tctgaccgtt gccgactggc atacttactt cgtcaagggt gatagggcgg aaacggaagg
      361 ggtttgggtt cataatgatt gtccgtatgg agaaaaatat aggacagaag ttggttctta
      421 tactaatact catgaaagtg gtcgaactta ttctggaaag ggcacaagac aacgttccca
      481 aatctctgga gcaagagaag ctagggttaa taatgaccct catatagcta cagattttac
      541 tcctgccaaa aataatcgag aggcattcaa agatgaatct agaagaattg acgctcacgg
      601 tggttccaaa agtaccaata attacaatcg aattgaaagc ccggggaaaa aataccgaaa
      661 acaagatgga gattaaaaat gattgttgac gaaaaatcta cttcagatgt actgaaacaa
      721 tatcaagaac tagggtttga tttgacaaaa cctatgataa tagagttttt tataggaggt
      781 tctcaaaaga atctgcaatc aatagaaagc cgactcattt cttatcggca agattttcaa
      841 atcagtattg agcaaaatga attcggagaa atgtggactt gctattgttc cgtcaatatc
      901 gtcccttccc ttgaaaacat tttagctatt gaaactacgt tatttggtat tgcacaaaaa
      961 aatgattgta attatgaagg ttttggttcg tatgggaact aacaaaaagt gtaggttggg
     1021 ttgaaaaaac catattccca ttaaagatta gggtgtgtct atggacataa aattcaatac
     1081 tttgggggtt atcctgaatg gggtcaatcc tgaagaaaaa ttcataaaaa ttattgatga
     1141 tcaggaaaat accgggggat ttctgatact gttatcttca aatgacaaat tctcatcatt
     1201 tgacagttac gatgactggg tcgaaaactt agaaatatta aaagaatacc tgcaagaatc
     1261 tcattggata ataaagtggg tcggatagtc ggcaaacgta gtccgtctga aacgctttta
     1321 cttttcagac ggactttcta tcaatgggaa caaggcgttt tgtctcaatc tggtctatga
     1381 ttattcgata cagtgatggg aagctaagaa aaatatgatt aatcatattg aaaaatatat
     1441 aggcactatt tctcatggct cgaaaagcga ttcgggaagt cagtacaaat taaatatcgc
     1501 agctattccc tcttccccta acagggattt aaaaacttat attactttag agttaagcaa
     1561 acatgatctg aattataaaa gcagatttga aatacttttt gtatgttctt taaaatatga
     1621 tgaaaatcag attttcccat ttttaagatg gctggcagaa accatcattg agaataaaaa
     1681 aatccttctt cgaggacagg ttatttattt gcctagaagc attgttgatt caacaaaaat
     1741 ggatgcactg tatgtttccg ccccatttta tttcgacgat gattttcagg tctgttatgg
     1801 cgaacactac aatatcgttt tccctttgct tgtccccttg tataaacagg aagccgaatt
     1861 ggtggaaaaa aagggttgga atgcttttga gcagttcttg ctggataatg aagttgacaa
     1921 cctttcggat atgaatcgga aaccgtttgt ttggtaaggg ctttcttaaa tttgattaaa
     1981 ggtcgtctga aaccgtgttt aactttcaga caacctttcc ttcatttgaa acaggatatt
     2041 gggaactaag ttcttcaaaa atcctgtctc cgtcccttat acgtcggtgc tttagtcaaa
     2101 acggcagacg gctacaaagc catcgcacat atccaaactg gggagtacgt ctttgccaag
     2161 gacgaagcaa gcggaaaaac gggatacaaa cccgttaccg cccgatacgg caatccgtat
     2221 caagaaaccg tttacattga agtttcagac ggcatcggca acaaccaaac cctgatttcc
     2281 aaccgcatcc atccgtttta ttcgggcggc aaatggatta aagcggaaga tttgaaagcg
     2341 ggaagcaggc tgttatccga aagtggcaaa acccaaaccg tccgcaacat cgttgtcaaa
     2401 ccaacaccgc tcaaagccta caatctgacc gtcgccgact ggcataccta cttcgtcaag
     2461 ggtaatcagg cggaaacgga aggggtttgg gttcataatg attgtccgta tgataaaggc
     2521 aaccaacgat ataaagacgc ttcttatcat ggcaaaaatg ataattctgt gaaaagtaga
     2581 gcaccaacaa acggacaagc agctcttgat aattccgttc aagttaaatc aacttctcct
     2641 cgaagagttg gggttgataa agccaataat gaaatcgttg tattagacaa aactcaaact
     2701 tttaataacg gttctgcgga atatcacggg catgtcagaa gttggcaaga tttgcatacc
     2761 gatcagaaaa atgctttaaa aaaagcagga ttggattagt taattcaaaa ggaaaaatta
     2821 aaaaatgact gataaaagta aaacagaaaa gttgatttct tctgatgata aacaaagtgt
     2881 tatagatggc attcttgata tggtatttaa ttccaaagca tatgaagtac cgtggatttc
     2941 tgagaaattg atggaattat cgaaaaataa agacttggat attgccggat tatcgctaac
     3001 ctgtttcgga catctcgcca ggctacattc aaatatcggt gattacgata aagttatttc
     3061 tttactacat tcaaagcaag atgatccaga gcttcaaggt agggctgaag atgcgttaga
     3121 agatatttct ttatttttat ctgaaaatca ttaggaaccg taggtcgggt tgaaaaccca
     3181 acaatcaaaa tgccgtctga aaccgtgttt aattttcaga cggcatttcc ttcatttgaa
     3241 gcaggatatt gagaactgag ttcttcaaaa atcctacacc tgctctttcc acggcagcac
     3301 cttggtcaaa acggcagacg gctgaaaagc aaacaccgtc cgtcgtgttg ccgtttgcgg
     3361 atgagtacgg gtcaacccca atgccgccga aaccgtcgaa gccgccttca acattgccgc
     3421 cgccaaagcc gcaaagttgg caaaaacggt aaaactgggg agataaaagc cgatggcagg
     3481 aaagtaaatg tgaggataga cagtacggag gcagacctgc tttatccggc agggcaataa
     3541 gaaaacaaaa attagatatg gaaaacgatt gtgaagatta aaccattaca attttctaac
     3601 aataatcaca gattttatgt ggacaaaatt tgtgttaccg aacaagatgt gaacattttg
     3661 gcatccgacc gaaattatga attaaatatt gaaatattta ttgacaatat aattcatttt
     3721 caaataacag atgaatctta taaagtaaaa ttttcagaat atttatttga aaataaagaa
     3781 aaaaatgatt gggatagaaa tcctgctata aattattttt tcgagataat agatgacagt
     3841 tatatggact ggttgaaaga agaaagtttt gatttttttg aaaagaaata ttataaggct
     3901 tatattttct tttttagcga ttctgtaata gaagttatca gctcgacaga acctgtattt
     3961 tattcaaaat aacaaattat caaacaaagc tctgattaaa aacccaacaa tcaaaatacc
     4021 gtctgaaaca atattcggct ttcagacggt atttttgaca caaagcaggt aaccaaagga
     4081 gtgtttgacg gaaaaggaga agctaaaata ccggatgtat cggttgggaa gcaatggata
     4141 aaggtaaata attatgtgga aaattagtaa agaaaattgt gaagatttag gatttgcaat
     4201 agtctgtatg ttctatgatg ctattaatct ttctgaattt aaattatggt tggatatagt
     4261 tgtcagagat actcctattg atacaattcc attgtatatt tttgatttga ttgattttga
     4321 taagagtata ggggaaattt atgatgtaat tggagtcgtt aattatggtt acatttcaaa
     4381 tgatcaaaaa aatgcattaa cgggcattgc cttcttaagg gggatagatg tctatgatcc
     4441 gcctatttca aaagaaaaag cattaaaagc cttagagaaa tatcctgaaa tttatcagag
     4501 gtttcagcat ttctttcctt ttgtagagct tccgcttctt taaaggacaa gatgccgtct
     4561 gaaaagtttt cagacggcat tttttatttc ttccagtagg cgggggtgaa gaggatgaag
     4621 acggtgaaga tttccagcct gcccaagagc atggcggtaa cgcagatcca tttctgcatc
     4681 acgtccaaac cggcgtaatt gccggcgggc ccgacttcgc ccaggccggg gccggcgttg
     4741 gtgatgcagg cgatgacggc ggtgaaggcg gtggtaaatt ccataccgct cgccatcagc
     4801 aggaagctga agaggacgac ggtcataaag tagatgaaga tgaaggacat aacggtcagc
     4861 gcgaggcggt cgggtatggc tttgccgctg attttgacgg tgcggacggc tttggggtgc
     4921 agcagcacca tcatttcgcg caggctgaat ttgaacagga cgagggcgcg tatggttttg
     4981 atgccgccgc cggtcgagcc ggagttggcg aggatgttgg cgaggaaaaa catccacagg
     5041 gaaatcagga gcggccattg tgcgaagtcg gtgttggcca gcccgtttgc cagtccgatg
     5101 gagacgaagt tgaaggcggt gtagcgcagg gattcggtaa aaccggcgta atagccggtg
     5161 tgccacaggt acagggcggc ggtaaggatg ctgccggaga gcagcagcag catcgtccgg
     5221 cattcttcgt ctttccaata ggttttgagg ctgcggctgt tgagggcggc gaaatggctg
     5281 gcaaaattga tgccgccgac aatggtgaaa acgatgatga ccgcctcgat gaggggggag
     5341 ttgtaataag ctatgctggc atcgtgggtg gaaaatccgc ccagcgagag ggtcgccatt
     5401 gcgtgacaga cggcatcgaa ccagctcatc ccggcaaaat gcaggcaggc tgccgcgagg
     5461 atggtgatca gggtgtagcc gaaccagagt tttttcgcca cttgggaaat gcgcggcgac
     5521 attttgcttt ctttgtcaat gccggggatt tcggctttga ataactgcgt gccgcctacg
     5581 ccgagcatag gcaggatggc gacggcaagg acgatgatgc ccatcccacc cagccagttg
     5641 agcatatgcc gccaaaagtt gacggagggg gcgagcccgt cgacgtgggg gatgacggtc
     5701 gcgccggtgg tggtcagtcc cgacatcgat tcaaaaaatg cgtcggtaaa gcccatattc
     5761 gggaaataca ggtacatcgg catcgccgcc atagcggcaa acgccagcca caacatcagg
     5821 acgagggtga agccgtcgcg cgggcgcagt tcgcgcctga accggagggt ggcgagccgg
     5881 acgatgtacg agccggaaag ggtaacggtc gcggtggtgg cgaaggcggt gtacgcgccg
     5941 tccgaaaagg cgtaggagag ggcggcgggt atcagcagga taaaggaaaa cagcataccc
     6001 agtcgggaga ggacgtgggc gatgggcagg attttgtgca tagtggggcg gtccgttatt
     6061 ttgcgaagct tttccagtct atgccgccgg cggcttggac ttgggtaatt tcttccgttt
     6121 cgaggttgac ggcggtcagc tgtccgcccc acagcgcgcc ggtgtccagc gagatgacgt
     6181 tgtcggcatt cgtgtagccc agcgaggacc agtgtccgaa gatgatggtg cggtcgaggt
     6241 tttgccggtc gggggctttg aaccacgggc gcaggtaagg cggcattttt ttcactgtgg
     6301 atttgtagtc gaaatccagt tcgtttttaa aggtcagggc gcgcatccgc gtgaaggcgt
     6361 tgacgatgaa gcgcaggcgg gcatagcctt tcaaaccttc gtcccacgcg gccggtttgt
     6421 tgccgtacat tttggagaag aatttgacgt attttttgcc gcgcagttcg gcttcggctt
     6481 ctctagcgag cgattcggct ttggttatgc gccattgcgg caggatgccg gcgtgtacca
     6541 tcacgcggct gccctcgcgt atcaacagcg gttgcgcacg cagccagtcg agcatttttt
     6601 ttccgtcggg gtgtttgagt atgggttcga ttgtgtcgct gcgtttgggc gcaccttcgc
     6661 cgcagccgac agcgagcagg tgcaggtcgt ggttgccgag gacgatttgc acactgtttt
     6721 cgtgccggat gcagaattgc agcgtttcga gggatttcgg gccgcggttg acgatgtcgc
     6781 ccgtcagcca gagggtgtcc gtgccgtggt tgaaaccgat tttgccgagc agcgcggtca
     6841 gttcgtcgaa acagccttgt atgtcgccga ttgcgtaatg tgccattgca gatgttgtga
     6901 agtgggaaag tgttgcggtt cggacggcat ggttttgaaa tatcatgcag tccgaacgtg
     6961 gaattatgcg ttcaaaacga ggacggcttc ggcttcgacc tgcacgcctt tgggcagcga
     7021 ggcaacgccg acggcggcgc gggcggggaa gggctcggca ataaattccg ccatcacttc
     7081 gttgaagacg gcgaaattgc ccaagtcggt caggtaggcg ttgagtttga cgatgtcggc
     7141 cagcgtgccg cctgccgctt cggcgatggc ttgcaggttt tggaacactt ggcgcgcttc
     7201 ggcgcggaaa tcgccgttgc cgacgacggt catcgtggcg ggatcgaggg ggatttgacc
     7261 gctcatgtaa acggtgtcgc ctgctcggac ggcttggctg tacgcgccga tggcggcggg
     7321 ggctttgtcg gtgtggatga tggttttgga catttcggat tcctcaaaaa atagggcggc
     7381 agaagccgca gcattcggga ttatcgtaca aaaccgccgg cttgtgtagt tgcggtggca
     7441 gaaaacaaaa ccgccgaagg ctcggcggtt tgcagaataa ggcgcatatc agaatttgac
     7501 gcgcacgccg acggacagtt cgccggaacg gacgtttttg acagtgttga ctttgccgat
     7561 gtagttgtag cggtagctgg catccaaatc gacattcggg gtaacggcat agcttacgcc
     7621 cgtcaatacg ccgaggccga tggaggtttg gctgaagctg tcgctgccgc ccaagtcgac
     7681 ggaggcgcgg ttgaggctca agcgcgcgcc gagatacggt ttgacgggcg attgggtgtc
     7741 gaagtcgtaa atggcggacg cgccgatgct gtaaagtttg aaatcggtgg atggggcttt
     7801 atagtttttg tagcgcgtgt aatcgacggc gaagcggagg tcgttgatgc ggtagcctgc
     7861 ggagatgcgc gggctgaagc ctttggcaga acctaaagag cttgaggctt ttgcgtgtgc
     7921 ggcatcggct tggacgtaaa agccggatgc gccttccgcc agtgcggcgg ccgggatagc
     7981 gagggcaatc agtgtggcaa gtgctttttt catattttgg ttcctttatg gtcagttaga
     8041 aaaattgtta agaatccgtt aaagaatcct gctgtattat acttaaattt tctttttgca
     8101 tcgtaatatt ttcaatactt caagatacgt agcggtatcc ggctgctttg ccgacggcaa
     8161 agccgttaac ccgcgcgttg cctttaaatg gtggcggcgg catcacgcgg cggatgggtg
     8221 aaacttgcaa acggtttgga aaaaacagcg gtatctgtcg gattgttgca ggtgcaggca
     8281 tacggttttg tgtgcgtctg tgccttaagc gtcggacatt tccggcggcg gctgtgccgt
     8341 ctgaaacgcc cggcggggga tgcggctgcg ttttccatcg ataagcatat tttccggacg
     8401 cgttcggggc gggttttccc gggcggccgc cgatttgttt gcgcttatat agtggattaa
     8461 caaaaatcag gacaaggcga cgaagccgca gacagtacag atagtacgga accgattcac
     8521 ttggtgcttc agcaccttag agaatcgttc tctttgagct aaggcgaggc aacgccgtac
     8581 tggtttttgt taatccacta taaaaaacac tgcagcaaat cgtttaaaaa caagcgtcct
     8641 ttttcggtcg ggcggaatac ggcagggtcg gtttccagca ggcctttttg ccttgccgtt
     8701 tcgatttgcg ccatgatttt ggcactcggt acgcccgtgc gctcctgcaa catcgcggcg
     8761 ggtacgccgt cggtcaggcg cagggcgttc atcatgaatt cgaacggcaa atcttcggcg
     8821 gcaacggttt tgcgttcgac ggcttcactc ggttggcttt gcattaaggc gaggtagtcg
     8881 ttggggtggc ggcggcggac ggtgcgctcg atgcggtcgg gataggagat tttgccgtgc
     8941 gcgcccgcgc ctatgcccaa ataatcgccg aactgccagt agttcaaatt gtggcggcac
     9001 cgcatggcgg gtttcgcaaa agccgatgtt tcgtaatgga taaagccggc gttttccaat
     9061 gcgccgtgta ccgcgtcttc gatgtcgagg gcggcttcgt cttgcggcaa acctttgggc
     9121 ggcgtgtgtc cgaacggcgt gttcggttcc atcgtcaggt gatacgcgct gatgtgggtc
     9181 gcacccgtgg cgatggcggt ttgtacgtca tccaatgccg tccgaacggt ttggttcggc
     9241 agggcgtaca tcaagtcgat attgatttta tcaaataatt tcagggcagt agctatggcg
     9301 gttaaggctt ccttgccgtt gtggacgcgc cccagccttg agagcatatc gttgttgaaa
     9361 ctctgcacgc caatagaaag ccgcgtgatg cctgcgtctt taaatccttg aaatttctcg
     9421 atttcaaacg tacccggatt ggcttccaag gtaatttccg cttccggctg caagcgcaac
     9481 agcgaacgca cgccgctcaa caaacggtca atcgattccg cctgaaacag gctgggcgta
     9541 ccgccgccga aaaataccgt ttccaccggc ctgccccaaa tattgggcaa ttcgagctgc
     9601 aagtcggtca gcagcgcgtc gatataggcg gcttcgggca atccgttttt caggctgtgg
     9661 gaattgaagt cgcaatacgg gcattttttg atgcaccacg ggatatggat gtaaagcgac
     9721 aggggcggca gggcggtgag ccgggtgcgg tttggaaagg aaatggtgtg catggtgcgg
     9781 ttcggaaaag tggacaatgc cgtctgaagg cggttcagac ggcacaggtt cagccgagca
     9841 gggtaagcag ttcggcttcg ctgaggacgg aaacgcccaa ggcattggct ttttccagct
     9901 tgctgcccgc ggcttctcca gcgacgacgt aatcggtttt tttggaaacg ctgccggaaa
     9961 ctttgccgcc tgcggcttcg attagggatt gggcttggtc gcgtttgagg gtgggcagtg
    10021 tgccagttaa cacgaaggtt ttgcctgcta cggcttcatt gaggccgtct gaaccttgcg
    10081 ccgcctcgtc ttcagacggc atttgcgcga agaaggtttt caggttttcg agtagggcgg
    10141 cgttttgtgg ttcgctgcgc catgcttgcc agtcggcggg gagggctttg tcgttttgta
    10201 gaccttctat gtttttacca gcgagttccc ataaggcttg ggctttgttt tcgctaattt
    10261 tgaagtcggg caggcgggcg atccagcgtt gcggttcggc gtggcgggcg ggcgggatgg
    10321 taacggcttg ggtttgcggg gcaacgcctg cggcgagcag ttcgtctatc atcgcctgct
    10381 gttcggcttg ggcgaagaag tgggcaatgg aatgcgccac taccgtaccg atgtcgggca
    10441 ggcaggcgag gacgggttcg ggggcgcggc ggacgcgttc caatgtgccg aatgcctgcg
    10501 ccagcgtttt ggcggtgcgt tcgccgacgt ggcggatgcc gagcgcgaac aggaagcgcg
    10561 cgagttcggg cgttttgccg gattctatgc ctgcgaggat gttttccgcc cacttggtcg
    10621 gttgtttttt atgtttgccc gacgcaccga ccgaactgcc ttcagacggc atttgatccg
    10681 attcggcaac ggttttgtcc gctgtttcct tcattttttg caaggtcggg atgtcgaggc
    10741 ggtagagatc ggcgaagtgg cggacgaggt cttgcgcgac aagctgttcg atttgttttt
    10801 cacccaagcc gtcgatgtcc atcgctttgc gcgaggcgaa gtggattaag ccttgcgcgc
    10861 gttgtgcctg acaaagcatg ccgccgctgc atcgggcgac ggcttcgcct tcttcgcgtt
    10921 cgatttcgct gcggcagatg gggcagtggg tcggcaggcg gtagggcttg tggagcggaa
    10981 cggattgggt ttgattggcg gacggtgttt cggcaaacaa atcgtcctgc cgatgcccga
    11041 tgccgtctga aacggcaacg gcggtttccc gcatcgggcg gcgttcaaaa atcacgcgca
    11101 caacttcggg aatcacgtct ccggcacggc gtacgacgac ggtatcgccg acgcgaacgt
    11161 ctttgcgcga tacttcgtcc tgattgtgca gggtggcgtt ggtaacagtt acgccaccga
    11221 cgaatacggg ctgtaatcgg gcaaccggcg ttaccgcgcc cgtcttgccg atttgcacgt
    11281 caatcgcttc gacaatggtc agggcttctt cggcagggaa tttgtgggca atcgcccagc
    11341 gcggcgcgcg ggagatgaag ccgagttcgt gctgttgcgc caagctgttg actttgacga
    11401 ccatgccgtc gatttcatag ggcagttcgg ggcgtttctg ctgcatttgt tcataaaacg
    11461 ccaatacttc gtcgatattt ttgaagcagc cgaaattgcc attgggcagg ctgaagccga
    11521 gtttttggaa ataggcgagt tcttggatgt gttcttccgc gacgaaaccg ccttgccggc
    11581 gggcgacgga gtaggggaaa aagtgcagtt tgcgttgcgc agtgatgcgc gaatcgagtt
    11641 ggcgtaggct gccggcggcg gcgttgcgcg gattggcaaa gggtttttgc ccgttttcgg
    11701 cttgtctttt attgagggcg gcaaaatcgg ctttttgcat cagtacttcg ccgcgtactt
    11761 cgatgagttc gggcgtattt tcgccgtgca gccgcaaggg gatgttggat acggttttga
    11821 tgttttgggt aacgtcttcg cccgttgtgc cgtcgccgcg cgttgccgcc tgtaccaata
    11881 cgccgtcgcg gtagagcagg ctgatggcga ggccgtcgaa tttaggttcg ataacgtatt
    11941 cgggtttgcc gccgtccaaa ccgtcgcgca cgcgttggtc gaaagcgtac atttctgcat
    12001 ggtcgaacac gccgttttca tcttgcgggg aaaaggcgtt ggtcagcgac agcatcggca
    12061 cttcgtggcg tacttcggca aatcccgcca gaggctcgcc gccgacgcgc tgggtcgggc
    12121 tgtcgggcag tttgagctcg ggatggtttc gttccaacgc ttcgagctcg cggaataatt
    12181 tgtcgtattc ggcatcgggt acgctgggcg cgtcgagggt gtagtattcg taggcatagc
    12241 ggttgagcag gtcggtgagg tggcggatgt gttgtgcggc aaattgtttt atatcactat
    12301 cagacggttt aagaagattg gtaaagttag tgttatgttt tgagtttgga ttcatgagag
    12361 aaggttttca gacgaccttt gtctgatacg ggatgaaacg ggcaaaggtc gtctgaaaaa
    12421 tgataggttg aaaacagctg aattttaccc gaaaaaaagc ggatatgccg taacgacata
    12481 tccgctttga ttgcattcga ttttaggaga acaggcgcag ggcggttttt cctccgggct
    12541 ggataccgac tttgagcatt tcctcctgac gtgccaaaac gtaggtgcgt acgtctttaa
    12601 gccattgggt agatacttct tccattttgt cgttaaccaa attgaggttc agttggccgg
    12661 acagtcggac tgccaaatcc ataaagaggt cgtcaaaagt tttttcgcct gccggggagt
    12721 gcggaatatc gagcagcata ctgaagcctt tgtaggactg gttgtccaaa agggcattgg
    12781 taaacggctc gttgttgagc gagcagatgg agaacatggt cgaacccgac gtgtcggtat
    12841 agtggaacgc gccgtcgtct tccaaaacga aacccacgcc cgttacggcg gaacgcagtt
    12901 ctacgccgct gatgctggtc ggggaaacca aatggatggc gatggtctga tcgacgcgcg
    12961 cgcagaatgc gtccagtgcg gaagccactt cgataaaggc ggcaaggtcg gtgtgcagcg
    13021 tctgaccacc catgctttgt gcgaatgcgt ccacctggcg gttgaatgcg gagagttctt
    13081 cctgcgaggc aagtccgttg cggctgactg cctgaatacc cacgataaat gcctgatagc
    13141 ggatgcccgg gatgggttcg gcaatctgga aatggtcgtc catggtgcag ccgacaatct
    13201 ggtagcggca gcggttggaa aggcgcggca gtgcgtgcag ttctttggct tcggtcagcg
    13261 cgatatagga gatgaagtcg aagcgcacgt caaaccaggg cagctcgacc ttcgacagct
    13321 ctttgagcgt aatcagcggt tttgtcggcg tttgcgggac aggaatgggt tttgccggcg
    13381 cgtcggcagg tttcggtgcg gaatgtccgg tttggggttc ggaaacggtg tgggcggagt
    13441 tgccgataat gccgctttct tccaaggcgg tttcgatttc ggttttgaac ggggaggctt
    13501 ttgcctgttt ctgcttggcg atgtagacgg catcctgttc ttgcaggttg cgcatggcgg
    13561 gatcttgggg ttttgccgtt tttttgaccg ccggttgggg tttcggcatc atgactggcc
    13621 cgccggacgg tttgccgtcg cggacatggc tggttttgct gttgagcagg gcatctttgt
    13681 cggagtgtcc gaactggtcg cgcacttttt tgcggtattg gttttcctga tacatattgt
    13741 aggcgacaac ggcgaggacg gcggcgagga acagtacgat gtaaatcatg gcaatcactt
    13801 taaatttcgg gatgcaggat acgcaaagtg cgggtactgc ggttaaatcg ggcttgcgtt
    13861 tccggcagtc tgacggaacg gccggttata acgtttgaat tataacgaaa attgcagggt
    13921 ctgacagcag tgtgtcgaaa taagcggaaa ttttccgaaa tgccgtctaa aatctgtggt
    13981 tttcagacgg catttctgtc cacgggaaac cctttccccc gtatccgccg ccagtcgaaa
    14041 aaatgaccgg ggtcggtttt gcggccgggc gcgatgtctt ggtgccccgt taccgccgtg
    14101 atggggtagc ggcggcagat tgcgtccaac aaggcttcga gcgaacggta ttgcgcttcg
    14161 gtaaagggtt cgaaatcgca gccttccagt tcgatgccga tggaaaatgc gttgcatttt
    14221 tcccgtccgc gaaacgagga tacgccggca tggtatgcca tattgtcgca ggaaacgaac
    14281 tgtaccgttt cgccgtcgcg tttgattaaa aaatggctgg atacgcgcaa agtgtgtatc
    14341 aggctgaaga acggatgtcc gtcggggtcg agccggttgg caaacagctt ttccaccgca
    14401 tccgtgccgt atttgaaggg cggcagcgaa atgttgtgca gcacgatcag ggaaaccgtt
    14461 tcccccgttt ccctcgggct gaaattgggc gacggggtat ggcgtatgct ttgaagccag
    14521 ccgttttgcc agtgtgcttc ggcgtgattg tccatgatgt tcttcctgtc cggcgggtaa
    14581 tttgggttat actgtcgccc gaattttaag acgtattccg aatgctggga atcctaccat
    14641 gttgaaaaaa atgttgaaat ggactgccgt ttttttgacc gtatcggcag ccgttttcgc
    14701 cgcgctgctt tttgttccta aggataacgg cagggcatac cgaatcaaaa ttgccaaaaa
    14761 tcagggtatt tcgtcggtcg gcaggaaact tgccgaagac cgcatcgtgt tcagcaggca
    14821 tgttttgacg gcggcggcct acgttttggg tgtgcacaac aggctgcata cggggacgta
    14881 cagattgcct tcggaagtgt ctgcttggga tatcttgcag aaaatgcgcg gcggcaggcc
    14941 ggattccgtt accgtgcaga ttatcgaagg ttcgcgtttt tcgcatatga ggaaagtcat
    15001 cgacgcaacg cccgacatcg aacacgacac caaaggctgg agcaatgaaa aactgatggc
    15061 ggaagttgcg cccgatgcct tcagcggcaa tcctgaaggg cagtttttcc ccgacagcta
    15121 cgaaatcgat gcgggcggca gtgatttgca gatttaccaa accgcctaca aggcgatgca
    15181 acgccgcctg aatgaggcat gggaaagcag gcaggacggg ctgccttata aaaaccctta
    15241 tgaaatgctg attatggcga gcctggtcga aaaggaaaca gggcatgaag ccgaccgcga
    15301 ccatgtcgct tccgtcttcg tcaaccgcct gaaaatcggt atgcgcctgc aaaccgaccc
    15361 gtccgtgatt tacggcatgg gtgcggcata caagggcaaa atccgtaaag ccgacctgcg
    15421 ccgcgacacg ccgtacaaca cctacacgcg cggcggtctg ccgccaaccc cgattgcgct
    15481 gcccggcaag gcggcacttg atgccgccgc ccatccgtcc ggcgaaaaat acctgtattt
    15541 cgtgtccaaa atggacggca cgggcttgag ccagttcagc catgatttga ccgaacacaa
    15601 tgccgccgtc cgcaaatata ttttgaaaaa ataaaccatg ccgtctgaaa agtttgtgtt
    15661 ttcagacggc atacccttac cggaactgca agcatgaaac cgcaattcat cactttggac
    15721 ggcatagacg gagccggaaa atccaccaac cttgccgtca tcaaagcatg gtttgaacgg
    15781 agggggctgc ccgtgctgtt cacgcgcgag ccgggcggaa cgccggtcgg tgaggccttg
    15841 cgcgaaatcc tgctcaaccc tgaaaccaaa gccggtttgc gtgcggaaac actgatgatg
    15901 ttcgccgcca gaatgcagca tattgaagac gtgatattgc ctgcactttc agacggcatc
    15961 catgtcgtgt ccgaccgttt taccgatgca accttcgcct atcagggcgg cgggcgggga
    16021 atgccgtctg aagacattga aattttggaa cattgggtgc agggcggttt gcgccccgat
    16081 ttgaccctgc tgttggatgt gccgctggaa gtgtcgatgg cgcgtatcgg acagtcgcgc
    16141 gagaaagacc ggttcgagca ggaacaggca gatttcttca tgcgtgtgcg cagcgtttat
    16201 ctgaaccgtg ccgccgcctg tcccgaacgg tacgccatta tcgacagtaa ccgcagcttg
    16261 gatgaagtca gaaacaacat agaaaaagtg ttggacggac atttcggctg ttaatgcggc
    16321 aaatattgaa acaagcgcat ccacccgcgc cgaaaatcaa acggcagtgc cgcaggtgaa
    16381 aatggcggta tgcgccaaac tttcggcatg atagaattac gctcggttac aaggcaggat
    16441 gcgtcggcaa tattaacgaa ccgcccgtaa catgatgacc cgaaagcgtt tcggacagtc
    16501 cgattcaaat ctttttctcg caacaggatt gacacatgga aaactcattg aaagaagccg
    16561 ccctcaagtt ccacgaatta cccgttccgg gcaaaatttc cgttaccccg accaaatctt
    16621 tggcgaccga caaagatttg gcgttggcgt actctccggg cgtagccgct ccttgtatgg
    16681 aaatccatgc cgatccgcaa aatgcctaca aatacaccgc caaaggcaac ttggtcgccg
    16741 tcatttccaa cggtacggcc gttttgggct tgggcgacat cggcgcgctg gcgggcaaac
    16801 ccgtgatgga aggcaaaggc gtattgttca aaaaattcgc cggtgtggac gtgttcgaca
    16861 tcgaaatcga tgaaaaagac ccgcaaaaac tcgtggacat catcgccgct ttagagccga
    16921 ccttcggcgg catcaacctc gaagacatca aagcacccga gtgtttctac atcgaacgcg
    16981 aattacgcaa acgctgcaaa atccccgtat tccacgacga ccagcacggc acggccatca
    17041 ttaccgccgc tgccgtattg aacgccctgc gttttaccgg ccgtaaaatc gaagaagcga
    17101 ctttggtgtg ttccggcgca ggtgcggccg cgattgcctg cttgaaccaa ttgctggatt
    17161 tgggcttgaa acgcgaaaac gtgaccgttt gcgactccaa aggcgtgatt taccaaaccc
    17221 gcgaagacaa agatcgcatg gacgaatcca aaaaattcta cgccgttgaa gacaatggcc
    17281 agcgcgtact tgccgatgcc gtcaaaggta aagacatctt cttgggcctc tccggcgcga
    17341 acctgctgac ccccgaaatg ttgaacacga tgaacgaaaa acccatcgtg ttcgccatgg
    17401 ccaacccgaa tccggaaatc ctgccgccgc tggcaaaaga aacccgtccg gacgtggtta
    17461 tcggtaccgg ccgttccgac ttcccgaacc aagtgaacaa cgtattgtgc ttcccattca
    17521 tcttccgtgg cgcgttggat gtcggcgcga cgaccatcaa cgaagaaatg aaacgctcct
    17581 gcgtgtatgc tttggcggat ttggcgatgg aagaagtaac cgaagaagtg gttgccgctt
    17641 acggtaagaa atttgaattc ggcgcggaat acctgattcc gactccgttc gattcccgcc
    17701 tgctgccgcg cgttgctacg gctgccgcca aagcagcgat ggaaagcggc gtggcaaccc
    17761 gtccgattgc cgactttgaa gcctacgccg ccaagctggg cgaatggaag ctgtaagccg
    17821 tttgcggttt aaaatgccgt ctgaactgtt ttcaggcggc attttgctgt cagattgata
    17881 aatgaaagat actggaaaat gaaagagatg gagactgtcc gttatcatat cggcgatatg
    17941 cccgagactt caaaacaaac cgcccctgtc atgacgacag ggcagtgggt gttgacgatg
    18001 attgttttca tgattccttt ggtcaatttt tgtttgggtg ttcggcagag gcaacccgaa
    18061 ccgcgccaat ttctgtaaag cgcagttgct tatttacctg attggttcgc ttatcggttt
    18121 ggtcttcgcg ttgtttatag gtgggtctgt atcgggtacg catgattaat gccccgggct
    18181 gattttgctt cgaggatttg tatcgaatat gccgaattgt ttcaaatttc ataccgttat
    18241 cgaacggcat tggcaaaaac cttatccggt tttgtctttt ctgcttaagc cgctctccgg
    18301 gctgtttgcc aaaattgcgg caaaacggcg gacggatttt ttatcgggaa aacggcaaag
    18361 cgaaaagctg cccgtgcctg tggtcgtggt cggcaacatc cacgcgggcg ggacggggaa
    18421 aacgccgatt gccgccgcgc tggtgtcggg tttgcaggaa aaaggtgtca aggtcggcat
    18481 catcagccga ggctacgggc gcaagagcaa ggcggttcat gtattgaatg ccgcgagccg
    18541 tgcggaagat gcgggcgacg agcctttgct gctgttcagc aaaaccggtg cgccgacggc
    18601 ggtgggcagc agccgtgcag aggcaggcag ggcgttgctg gcggcgcatc ccgacatcgg
    18661 actgattgtg gcggacgacg gtttgcagca ttacgccctg cggcgagatg tggaaatcgc
    18721 ggtgtttccg gcggcggata cggggcgcac ggatttggat ttgctgccca acggcaattt
    18781 gcgcgaacct ttgtcgcggc tggattcggt ggatacggtc gtcgtcagcg gcggcaaggc
    18841 ggatgcgctg tttaggccgt ctgaaaatat gtttcacagc cgtatcgaag cgggacgaat
    18901 ttaccgtttg aacaatccgt ccgaaatact ggacacaggc cgtctgaaaa atcaaaccgt
    18961 cgtcgccgtg gcaggtattg ccaagccggc gcgttttttc gattcgttgc ggaatatggg
    19021 cattaccgtg aagcgaaccg tcgcgctgcc cgaccacgcc gacatttcgg cggcagattt
    19081 gcccgatgcg gacgcggtca ttattacgga gaaagatgcg gtcaaatttt cagacggcat
    19141 ttgcaccgat aatgtttggg tgttgcccgt ttgtgcgata atcgaacctg atttggcggc
    19201 gtttgtgttg gagcggttgg aagatgtacc gaaggccgtc tgaaagcacg gtttgggcgg
    19261 agtgattacg gatttgaata agaacgcctc gcgccgtcat tcccgcgcag gcgggaatct
    19321 aagtctcgaa ttttcaggaa tgcctaggag gcttcagaaa tcccaaatct ccggatttcc
    19381 acttggacag gaatgagaaa accggtcgta ttttttatct gcattaatca ttcattaaag
    19441 gattgaatat taaactgaaa accttgttat tgcccttcgc cacgctggca ttgtgcacca
    19501 atgcttttgc cgctccgccc agcgacgcgt cgttggcgcg ttggctggat acgcagaatt
    19561 ttgaccggga tatagaaaaa aatatgattg agggctttaa tgccggattt aaaccgtatg
    19621 cggacaaagc ccttgccgaa atgccggaag cgaaaaaaga tcaggcggca aaagccttta
    19681 accgctatcg tgagaatgtt ttgaaagatt tgattacgcc cgaagtgaaa caggctgtcc
    19741 gtaatacttt attgaagaat gcccgtgaga tatacacgca agaagaaatt gacggcatga
    19801 ttgcctttta cggttcgcct gtcggtcagt ccgtcgttgc caaaaatccg cgcttaatca
    19861 agaaatcgat gagtgaaata gcagtatctt ggactgcatt gtcagggaaa atcgcgcgac
    19921 atcatctgcc cgcgtttacg gaagagttgc ggcgcatcat ctgcggcggt aaaaatcccg
    19981 atgcgggctg taaacaagcc ggacaggttg ggaaaaggca tcagaaataa atgatagccg
    20041 tctgaaatat tgaagagggc atccgattga ttgaatcatc aaacccgaaa gcaaccctat
    20101 ggaaaaaaaa ttcttagaca tcctcgtctg ccccgttacc aaaggcaggc tggaatatca
    20161 tcaggacaaa caggaattgt ggagccgtca ggcgaagctt gcctatccga ttaaagacgg
    20221 cattccctat atgctggaaa acgaagcgcg aacgttgagc gaagaggaac tcaaagcatg
    20281 accgaattcg tcgtattgat tccggcgcgg ctggattcgt cgcgcctgcc cggaaaagcc
    20341 ttggcggaca tccacggcaa accgatggtc gtgcgcgttg ccgaacaggc ggcaaaaagt
    20401 aaagctgcgc gcgtcgttgt tgccaccgac catcccgata ttcagacggt ctgtcaggcg
    20461 cacggcgtcg aagtcgtcat gacttcaaac cggcacgaaa gcggcacgac gcgccttgcc
    20521 gaagctgccg ccgcgctgaa gctgccgcaa catctggtcg ttgtcaacgt acagggcgac
    20581 gagccgctga ttgcccccga actcatcgac cgcaccgccg aagtactcgt cgaaaacaac
    20641 gtccaaatgg cgaccgccgc ccacgaattg cacgatttcg acgaattgat gaatcccaat
    20701 gccgtcaaag tcgtcctcga caaaaaccgc aacgccatct acttcagccg cgccccgatt
    20761 ccctatctgc gcgatgcaat gcgtgccgga aaacgcgaaa taccgtctga aaccgccgtc
    20821 ctgcgccata tcggcatcta cgcttaccgt gtcggcttcc tgcaacgcta tgccgaaatg
    20881 agcgtttcgc cgctggaaac catcgaatcg ctggaacagc tgcgcgtcct gtggcacggc
    20941 tacccgattg ccgtcgaaac cgccaaagaa gcccccgccg ccggtgtgga tacgcaggaa
    21001 gatttggaca gggtccgcgc cgtatttcag accgtataaa acaggttcaa agggaaaaga
    21061 tatgcagcaa catattgaaa agtggcagca cttgagccgt gaagaacaga aaatccttgc
    21121 cgaagtatgg ggtctcgtgc aaaacgacga tcaggaggtt cactatgaaa tgctcaaatt
    21181 gaacgcaccc gacgaagcca gtggcgaatt ttggttcaga atggcagaaa cactcagcac
    21241 cctgccgccc aaccgttccc tcgaccttag aatgaacggc ggcaggctgg cgaccgccgt
    21301 atccatcctt tccgtcatga ttgaagacaa tcccgacata ccgcagcttt gggcgcaaaa
    21361 aattaccgcc ctcaattata gtggattaaa tttaaaccag tacggcgttg cctcgccttg
    21421 tcgtactatc tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta
    21481 tatttggcac acggacacaa agcccgtgcc gacggtttgg cacaacagcc cgacaaagcg
    21541 gcagaagcca acgaggagga atacctgacc aaagccctgt cgcaaaacct gctgtcaaca
    21601 ttggatgtcg cgcttgcacg ttttcctgaa gacgcgtggt ttcaggaaat caaacaggat
    21661 gcacaaaagc attttgcttg aggatgtggc agtcaggaat atttccattc aggaagaaaa
    21721 gaagtgcctg attgggtata atcagggtaa atcttatttt atttcaaaag attaatattt
    21781 gctttccatt tttccttgac ggtatcggaa aagttgatta tagttacagc ttccttagga
    21841 gtaatggctg agaggctgaa ggcacttccc tgctaaggaa gcatgtgggg tcaacctgca
    21901 tcgagggttc gaatccctct tactccgcca agatgagaag acgctgtgtt ttacagcgtc
    21961 tttttttacg cgctttgttg cggaagtgcc gagggctgtc tgccatgcgc cgcctggatt
    22021 tgcaatgtgc ccgcccgtgc aaaatccgat ttgaccaacc tgaagggcag gtttcggaag
    22081 agcggggaat tttgataaac aaaaaactgc acggcataat ctttcccggt tttcattttc
    22141 cgtattcgcc tatccgtacc gcaaatgcta tactgggaaa aatttaccga ttgtgtttta
    22201 cggcatattt gccgatagga tggaagagac aaatgagcag aatccggcag gcttttgccg
    22261 ctttaaacgg tgaaaaggca ttgattccct atattacggt gggcgacccc gatattcgga
    22321 caactttggc attgatgcac ggcatggttg caaacggtgc ggatattttg gagttgggcg
    22381 tgccgttttc cgatccgatg gcggatgggc cggttattca gcgtgcggcg gagcgggcgt
    22441 tggcaaacgg gatttcgctg cgcgatgtct tggatgtcgt cagaaaattc cgtgaaaccg
    22501 acacgcaaac gccggttgtt ttgatgggat atttgaaccc tgtacataag atgggttatc
    22561 gggagtttgc tcaggaagcc gcaaaggcgg gtgtggacgg cgtgttgacg gtggattccc
    22621 ctgtcgaaac catcgatccg ctctatcgcg agctgaagga taacggggtc gactgtattt
    22681 tcctgattgc gccgacgacg acggaagacc gtattaaaac cattgccgag ctggcaggcg
    22741 gatttgtcta ttatgtttcg ctcaagggcg taacgggcgc ggcaagtttg gatacggatg
    22801 aggtttcgcg taaaatagag tatttgcatc agtatatcga tattcccatc ggtgtcggtt
    22861 tcggcatcag caatgcggaa agcgtgcgca aaatcggccg ggttgccgac gcggtcattg
    22921 tcggcagccg gattgtgaaa gagattgaaa acaatgcggg caacgaggct gcggtcgtcg
    22981 gtgctttggt caaagagttg aaggatgccg tgcgctgatt ctgtcacgca ttctaaatat
    23041 tttaggagtt gtccatgagc tggttagata aaatcctgcc acccaaaatc aaaaaccgca
    23101 gcaaagacgg ttcttccaat gttcccgagg gtctatggca caaatgcccg tcttgttcgg
    23161 caaccgttta ttctaccgag ttgcagcaga acaatcaggt ttgtccgaaa tgcaaccacc
    23221 acaatccgtt gtcggcacga caacgcctga acctgctttt ggatgaggat ggcagggagg
    23281 aagttgccgg aaatgtcaaa ccgacagatc ctttgaagtt taaagacggc aaaaaatatc
    23341 cggatcgttt gagtgcggca cgcaagctga ccggggaaga tgatgctttg gtggtgatga
    23401 aaggcaagat gaacggtctg cccgtcgtcg ttgccgcgtt tgaattccgc tttatcggcg
    23461 gttcgatggg ttcggttgtg ggcgaacgat tcgtacaagg tatccgtcgg gcggttgccg
    23521 acaattgtcc gttcgtctgt gtggcggctt ccggcggtgc gcgtatgcag gagggtgtga
    23581 actcattgat gcagatgacg aaaaccagtg ccgcgctgca tttgctgacg gaaaaacgcc
    23641 tgccatttat atcggtgttg accgatccga ctatgggcgg cgtatccgcc agcttcgcat
    23701 ttttgggcga tgtcgtgctt gccgaaccga acgcgctgat cggttttgcc ggtccgcgcg
    23761 tgattgagca gacggtgcgc gaaacgctgc cggaaggctt ccaacgcgcc gagttcctgc
    23821 tggaaaaagg cgcaatcgac cagattgtcg accgccgcga tatgaagcgg cgcatcagtg
    23881 atttgattac gctgttgtgc cgtcaggaca aagtttccgc cgcttgatgg ctgatgaatc
    23941 gaataccgtc tgaaaccgat gtttcagacg gtatttttgt gtctggttat ttgttgtgcg
    24001 gctttatcga cggggcatag cgtccggcac gttctttcag gcgttgtacc aaacctttcg
    24061 tgtcggcggg tacaccgccc tcgcagaatg cctgatacag gacggtgcgc agtgcgtcgt
    24121 tgcggcttaa tgtaccgcct atcggtttcc attcggcgtt tcggggctgt atccagcggc
    24181 ggttgaccgt gtcgccgtag ccgaacactt tataggagga aggtttgccg ttgccgaacc
    24241 tgatggagaa gcgggcgcag aatatgcctt cggcagtcag gttgtcgtag cctttgtcgg
    24301 agcggatatt gagaatgtag cggatgctgc cgtcgggcgc gggcataatt tgcaggctgt
    24361 cgagcaggat tttcggctgt ttgccgtaat tttcatccac ataaatgtcg aaccagccgt
    24421 ccgagtgcgt atcgggcagc ggcggcagtt tggcggtatg ttctttaaat tcgcgggcgg
    24481 cggcttcttc gggcgtttcg cggtagcggg tgttgatcgg cgtgtctttt tggctgaagc
    24541 cggcagcaag ggacgtgcca gcggcgagtg cgagcagcag gagggtggtg cggcgcataa
    24601 gtttctccaa attgaaaacg gcgttatttt atgggttggc aaagggggct gcaagcaact
    24661 ggggtataat ctccccccga ttcccatttt ttaaacggta caaacgatga acagcgaaac
    24721 tttagacgta accgggctga aatgcccttt gcctattttg cgtgccaaaa aggctttggc
    24781 gcaaatgcag cagggcgacg tgttgaccgt tctggcaacc gacggcggcg caccggggga
    24841 ttttgaggct ttttgccgcc aaaccggtca tgtgctgttg gattcttccg aacaggacgg
    24901 cgtgttcacg ctggtcgtca agcacaaata aatgccgtct gaaatgcgga tgtcccgccc
    24961 gacggcgttg tttttgaata tcgtatgtgc cgcgtgccgt ttcaaaaaca aaccgtccgg
    25021 cgtgcagtgt cggcatttcg ggcgtatcgc cgccgatttg ttccggaatg gctgttaacc
    25081 gccttgccgt cggcaaagaa gcaaaaccca agcacaatca aaatctaaag gctgtgtttg
    25141 aagattccgt tgatgcaacc caatcagtcg gacaaaatgc ctttcatcca atggaaccgg
    25201 tttccgaccg gacggaataa ccggctttcc cctggcaaac ggatggaatc gaccgggtat
    25261 tcaaacgcag ccaaaaccta aaaaggaaca accatgcaaa ccctgaccat tatccgcccc
    25321 gacgatatgc acctgcacct gcgcgacggc gacgcgctca aagccgttgc cccttatacc
    25381 gcccgccaaa tggggcgcgc cgtcattatg cccaacctca aaccgcctgt cgtcagtgta
    25441 gccgacgcgc ttgcctacaa agcgcgcatt atggcggcgt tgcccgaagg cagcgcgttt
    25501 gagccgttga tgacgcttta tttgactgat aacgccacgc ccgaacttgt acgcgaagcc
    25561 aaagccgccg gcatcgtcgc cttcaaactc taccctgccg gcgcgaccac caattccgat
    25621 tccggcgtaa ccgacctgtt caagctcatc cccgtgttgg aagaaatggc gaaacagggc
    25681 attttgttcc tcgttcacgg cgaagtaacc gaccccgaaa tcgacatctt cgaccgcgaa
    25741 gccgccttta tcgggcgcgt gatgaaaccc gttttggcgc aagtgccgaa tcttaaagtc
    25801 gtgttcgaac acatcaccac cgccgaagcc gcccgcctgg ttttggaagc aggcgacaac
    25861 gtagccgcca ccgttacccc gcaacacctc ctgctcaacc gcaacgacct cttggtcggc
    25921 ggcgtgcgcc cccatcattt ctgcctgccc gtactcaaac gcgaaaccca ccgtcaggca
    25981 ttggttgccg ccgttaccgg cgagaaggcg cacaaattct tcctcggcac cgactccgcg
    26041 ccgcacgcca agtccgccaa agaaaacgcc tgcggctgcg ccggtatgtt cagcgcgatg
    26101 accgccgtcg agctttacgc cgaagtgttt gaaaaagcag gcgcgttgga caaactcgaa
    26161 gcctttgcct cgaaaaatgg cgcaaggttt tacggcattc cggaaaacgc cggcacgatc
    26221 accctcgtca aacaaagcca aaccgtcccc gcaagtgttc cctacggcga cggcgaactt
    26281 gtcccgatgc gcgcgggcgg cgaaatcggc tggacggtac agtattgatt gaacggtcaa
    26341 gcaaaatgcc gtctgaaagg tttttcagac ggcatttatg tattttccaa ttcggatttt
    26401 ccggagtgtc aacggcgttt gggctcgtcg gggcggattt gggcagcgag tttgtcgagg
    26461 atgccgttga caaatttgtg cccgtccgta ccgccgaagg ttttggttac ttcgatggct
    26521 tcgttgataa tgacggggta gggtgtttcg ggcatggcgg aaagctcgtg gcaggcggtc
    26581 agcaggacgg cgcgttcgat ggggttgagg tctttttcgt ccctgtcgag cagcgggcgg
    26641 attttttgga tgtagtccgc tgcgttggtt tgcgtgccga agaaaagttt gttgaacaat
    26701 tcttcgtctg ccttggcaaa gtcggacatt tcgcggatgt ttttagcaat ttcgggcgcg
    26761 gcggtgcggt tgataaggga ttggtaaacg gcttgtacgg caagctcgcg agaacggcgg
    26821 cgggctgttt tcatgatttt tccttgaaac ggttgggcgg cacggtatgc cgtctgaaac
    26881 ggaaagggcg tatcggtgta cgccctgttt gttattcttc gtcttcaaac tgttcttcga
    26941 gcagcaggtt gacgaggttg gcgcattcga cggcgacttt ggcggcatcc gaggcttttt
    27001 cttcaatccg ttcgattgcc tgcgcgtcgt tttcggtggt taggacggca ttggcaatcg
    27061 ggatattgta gtcgagtgcg acgcggctga cgcctgctcc ggattcgttg gaaaccagct
    27121 cgaaatggta ggtttcgcca cggatgacga cgccgatggc aatcagtgcg tcaaactttt
    27181 cggaagaggc aaagttcatc agcgcgatgg ggatttcaag cgcgccgggt acggtggcga
    27241 cggtaatgtt ttcgtctgcc acgcccaatt cttggagggt gcggcagcag actttgagca
    27301 tttcgctgcc gatttcgttg gtgaaacgtg cctgtacgat gccgatgcgg aggtgtttgc
    27361 cgtcgaggtt gggggcgatg gtgttcattg ggtgtccttt ggtattcgga ggtttcggaa
    27421 tgccgtctga agggtcagtc cttaggttgc cagtcggcaa cggtttggaa tgtgccgtct
    27481 tctgccaatt cccacgcgct gccttcgggt tgggaaagca gtgcggcggt ttcagggttg
    27541 gttttggcga tgttggcgag gctgacgata ctgaagttgt cggggtcgtc ggtgtattcg
    27601 tcggtctcgt cgccgctgaa gaaacgccag ccgctgtcgt tttcaaaaac gggggcttcg
    27661 cggtagagga agccgacggg ctggttttgt ttggcgacgg tgttggtggc gatgcagcgg
    27721 tcgagtgccg aggaaagtgc ttgtgcaaat gcgttcattg cgggaatacg ttgggggaaa
    27781 acttgcggat tttaccacga ttcttgcgtt gtcggcagac ggcggcggtt tggtggtaca
    27841 atgtgcgccg tttgcagcct taaggtgttt ctgtattttt ggagtatgga aacgcattcg
    27901 ggctgttttt tgcggaagac ggtaatgaaa gacgatgttt tgaaacagca ggcacacgcg
    27961 gcgatacaga agagactggg ctacgcgttc cgcgatattt cgcttttgcg gcaggctttg
    28021 acgcacagga gccatcatgc gaagcacaac gagcggttcg agtttgtcgg tgattcgatt
    28081 ttgaattata cggtggcgcg gatgctgttt gacgcgtttc cgaagttgac cgagggcgag
    28141 ttgtcgcggt tgcgggcgag tctggtcaat gagggcgtgc tggcggaaat ggcggcggaa
    28201 atggatgtcg gagaaggctt gtatttgggg gcgggcgagt tgaagagcgg cggctttaga
    28261 cggccttcga tactggcgga cgcgatggag gcgatgtttg ccgccgtcag cttcgatgcc
    28321 gatttcaaca cggcggaaaa ggtggtacgc catttgtttg ccgatcgagt ccggtgtgcc
    28381 gatttccaaa atcaggcaaa agacggcaaa actgctttgc aggaggcgtt gcaggcgcgc
    28441 cgtttcgctt tgccgaaata ccgtatcgaa gagcaaatcg gccatgccaa cgacagtatg
    28501 tttgtcattt cctgcgattt gggcgaactg ggtttcgtgt gctatgccaa agggacgagc
    28561 cgcaaggcgg cggaacagga ggcggcgaaa gaggctttga aatggctgga aaagaagctg
    28621 ccgctgaaga agaaaaagaa atgaggcggc gcgtgaatat gccgtctgaa atatggatat
    28681 gaaagcgaat atggatattg aaaccttcct tgcaggggaa cgtgccgccg acggataccg
    28741 ttgcggcttc gtggcgattg tcggtcgtcc gaacgtgggc aaatcaacgc tgatgaacca
    28801 tctcatcggt cagaaaatca gcatcaccag caaaaaggcg cagacgacgc gcaaccgcgt
    28861 aacggggatt tataccgacg ataccgcgca gtttgtgttt gtcgatacgc ccggttttca
    28921 aaccgaccac cgcaacgcgc tcaacgaccg tttgaatcaa aacgttaccg aggcactcgg
    28981 cggtgtggat gccgtggttt tcgtcgtgga ggcgatgcgc tttaccgatg ccgaccgagt
    29041 cgtgttgaaa caactgccca agcacacgcc ggtcgtttta gtggtcaaca aaattgacaa
    29101 ggacaaggcg aaagaccgtt acgcgttgga ggcgtttgtt gcccaggtgc gcgccgaatt
    29161 tgaatttgcg gcagcggagg cagtcagcgc gaaacacgga ttgcggattg ccaacctgtt
    29221 ggagctgatt aaaccgtatc tgcccgaaag cgtgccgatg tatcccgaag acatggttac
    29281 ggacaaatcg gcgcgttttt tagcgatgga aatcgtgcgt gaaaaattgt tccgctattt
    29341 gggcgaggaa ttgccttatg cgatgaacgt cgaagtggag cagtttgaag aggaagacgg
    29401 tttaaaccgc atctatatcg ccgttttggt cgataaggaa agccaaaagg cgattttaat
    29461 cggcaaaggc ggggagcgtt tgaagaaaat ttccaccgaa gcgcggttgg atatggaaaa
    29521 actgtttgat accaaagtgt ttttgaaagt gtgggtcaaa gtcaaatccg gttgggcgga
    29581 cgacatccgc ttcctgcgcg agctgggttt gtagtttttc ttgctgaact ttacgcaaat
    29641 gccgtccgaa caggtttcag acggcatttt gtttcaatcg ggaatatctt tgttaaaaac
    29701 gggttgatat tatctgtgca
//
LOCUS       DAWFIE010000017        28506 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWFIE010000017 DAWFIE010000000
VERSION     DAWFIE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28506
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.017"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(82..1338)
                     /locus_tag="WGQ71_000718"
     CDS             complement(82..1338)
                     /locus_tag="WGQ71_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ0706768.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWNEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAIGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSVLPEENELQVERFLSEHPEFEPVNCAELLQSLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(1373..2230)
                     /locus_tag="WGQ71_000719"
     CDS             complement(1373..2230)
                     /locus_tag="WGQ71_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ0706769.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPLGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGSGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(2291..3106)
                     /locus_tag="WGQ71_000720"
     CDS             complement(2291..3106)
                     /locus_tag="WGQ71_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0706770.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            3170..3340
                     /locus_tag="WGQ71_000721"
     CDS             3170..3340
                     /locus_tag="WGQ71_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706771.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVRRYFTRFGRIYPNQINSDFAAP
                     KDCTSEPAVCHP"
     gene            3350..4135
                     /locus_tag="WGQ71_000722"
     CDS             3350..4135
                     /locus_tag="WGQ71_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ0706772.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGFHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLVILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDGWLESGDIVAANPKVLAQMLKIISAHV"
     gene            4394..6517
                     /locus_tag="WGQ71_000723"
     CDS             4394..6517
                     /locus_tag="WGQ71_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0706773.1"
                     /translation="MGKANAFTAPITVVNYDEQALNNTEARTLVDAVAKKDASVWQFG
                     GESNTLTGLYFRGYQLDSRQFSVNGLAGMYGTQGTASVQVGSAQLIKGASTAVNGMDP
                     EGAVSGSVNIETKKAADEGNRKIGLGWFSNNRAQGTFDLGQRFGENKAFGVRANGKLR
                     HGDTPRHGYSEDNKEFAVNADYRGETLRVAFDSIYAKRKTNGGRARMQDIQNAGGRLF
                     GAPDGKTNLLPSWNWQNTAGQTNMLTFEWDAFDNAQITGGIGYNKARYYGTLISPTVC
                     LNATSTCTDMQNTNASGKVTARNYDYNTGTARLTDQYFRTLSMNLSARGEFETGPVTH
                     NWSTAFDRVIRQRKTIRGTAAGAGKVEVKANENIANQLASFKADYPNSWENSANLDAN
                     IKVNSLALSDTLGFVDNKYRLTLGGRFQAVEYTNKKKSQSGDAKRFSPMLMAAWVPQP
                     DLVVYGNYMEDLEPADIKTDDSGETTMAKPRVSRQFEVGVRKNWGNFVTTLNAFQIKR
                     PGYWRGHTTTKTTKGVTTTLTYGNNSDFARYKAQGGAAGDEQGMERNRGIEFNTYANL
                     LNNTLRPTLGLMYLQSTVKEYPNSRDMLVNGVQVANPRVIAKAGLEWDTPFAKGLTLN
                     GNVSYFGKSYQDTQKQYAFPSYTLIDVGARYKTKLGKNTLTVSSSVENLFNKNYWQVQ
                     RGQYDRSFAVVGMPRTYWLKAELDF"
     gene            6641..8191
                     /locus_tag="WGQ71_000724"
     CDS             6641..8191
                     /locus_tag="WGQ71_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ0706774.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFINSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DLKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
     gene            complement(8551..10335)
                     /gene="lpdA"
                     /locus_tag="WGQ71_000725"
     CDS             complement(8551..10335)
                     /gene="lpdA"
                     /locus_tag="WGQ71_000725"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0706775.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAATTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(10412..12019)
                     /gene="aceF"
                     /locus_tag="WGQ71_000726"
     CDS             complement(10412..12019)
                     /gene="aceF"
                     /locus_tag="WGQ71_000726"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ0706776.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGTAIIEVETAGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQ
                     GGAAKPAAAGASLGSSLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPIWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(12169..14832)
                     /gene="aceE"
                     /locus_tag="WGQ71_000727"
     CDS             complement(12169..14832)
                     /gene="aceE"
                     /locus_tag="WGQ71_000727"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ0706777.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIETERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            15526..17238
                     /locus_tag="WGQ71_000728"
     CDS             15526..17238
                     /locus_tag="WGQ71_000728"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ0706778.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDSYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVRSPLTMASPAAIVEQFGANGG
                     SLGPVSFAGKVYADFATEKGADWVIGANEDGYHYTSFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNA"
     gene            17301..17957
                     /locus_tag="WGQ71_000729"
     CDS             17301..17957
                     /locus_tag="WGQ71_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ0706779.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGMGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKAQGLPWLKAKGFRHSACVSDFAAAGRIGNPEKMLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVHASFTVGC
                     "
     gene            complement(17967..18422)
                     /gene="ruvX"
                     /locus_tag="WGQ71_000730"
     CDS             complement(17967..18422)
                     /gene="ruvX"
                     /locus_tag="WGQ71_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ0706780.1"
                     /translation="MHKAPKGTALAFDFGETRIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(18415..18963)
                     /locus_tag="WGQ71_000731"
     CDS             complement(18415..18963)
                     /locus_tag="WGQ71_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ0706781.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVDRGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALHGKAGHA"
     gene            complement(18965..19525)
                     /locus_tag="WGQ71_000732"
     CDS             complement(18965..19525)
                     /locus_tag="WGQ71_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ0706782.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            19853..21658
                     /locus_tag="WGQ71_000733"
     CDS             19853..21658
                     /locus_tag="WGQ71_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0706783.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKSVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNLKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            21785..23269
                     /locus_tag="WGQ71_000734"
     CDS             21785..23269
                     /locus_tag="WGQ71_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ0706784.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
     gene            23598..25625
                     /gene="uvrB"
                     /locus_tag="WGQ71_000735"
     CDS             23598..25625
                     /gene="uvrB"
                     /locus_tag="WGQ71_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ0706785.1"
                     /translation="MEVIRYPDSPFKLHQPFQPAGDQPTAIADLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRNDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTIFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERILVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEASVLRDRIRGIREGLLFGSE"
     gene            25737..26024
                     /locus_tag="WGQ71_000736"
     CDS             25737..26024
                     /locus_tag="WGQ71_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706786.1"
                     /translation="MTYSFRVENINDISDIPENHLEIVRAALPSNRSNALSREPDGAQ
                     AVFVYEIDGEFRAKLFDRIKDTDSYLQATEDNYSNDPRSIEVVYVLEQQQG"
     gene            complement(26205..26921)
                     /gene="trmB"
                     /locus_tag="WGQ71_000737"
     CDS             complement(26205..26921)
                     /gene="trmB"
                     /locus_tag="WGQ71_000737"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ0706787.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPVDLDANFGSNRPKILEIGFGMGTATAEIALRLPETDFLAIDVHDPGVG
                     NLLKLINENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTATDYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIR"
     gene            complement(27043..28446)
                     /locus_tag="WGQ71_000738"
     CDS             complement(27043..28446)
                     /locus_tag="WGQ71_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0706788.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLISLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYREAAAQGLAAAQWQLGLQYRFGQGTPTDTTQAINHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDKAVYRFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDICRYGLGMTSDKEKARHYYRQAAEAGSLTAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
BASE COUNT         6755 a         7074 c         7936 g         6741 t
ORIGIN      
        1 tttttgttaa tccactataa ttgatgaaat gccgtctgaa cgctttgaaa agggttcgga
       61 cggcattttg ttcaaaccgg tttatttgcg ttgcaatacg gcggcgaaga agccgtcggt
      121 ttggtgtcgg gcggaatcga ggcgcaggta ttttccggta tccaaatcaa ccttcaggct
      181 ttgcagcagt tcggcgcagt tgacgggttc aaattcggga tgttcggaca ggaaacgttc
      241 gacttgcagc tcgttttctt ccggcaacac gctgcaggtg gcgtacacca agcggccttg
      301 cggtttcacc agtttggatg cggcatcgag gatgctgtgt tgctgttcca aaaggttggc
      361 cacggtctcg gcggattggc ggtatttgag gtcgggattg cggcgcagcg tgcctaagcc
      421 ggagcagggc gcatccacta aaacgcggtc ggctttgcct gccagtcggg caatacgggt
      481 atcgtgttca ctgctgatgc gttcagggtg gatgttggtc agtccggcac gggtcatgcg
      541 cggtttgagg ttggcaaggc gtttttcggc gatgtcgaag gcgtagattc tgcctttgtt
      601 cgccatttgc gcgccgatgg cgagggtttt gccgcctgcg ccggcacaga aatcgacaat
      661 gatttcgccg cgttttgcgc ccaccaataa agcaagcagc tggctgcctt cgtcttggac
      721 ttccagtgtg ccgtccaaaa acagctcgtg tttgttaagc gcgattttgt ttttcaggcg
      781 gatgccccaa ggcgaataag gcgttgcctc cgcatcggca ctttcggctt gcaacaatgg
      841 cagcacttta tcgcgtttgc ctttcaaggt attgacgcgg atgtcgagcg gcgcggcttg
      901 gttgatgctg cgtccgaagg cgaggatttc ttcttcgttc caatgctgtt gcagttgttc
      961 gaccagccat tgcggcaatt ctgcggcggt attcagaccg tctgaaaact cggttttgcg
     1021 ggctttcaaa ttgccgagga actctgtttc ttcttcatca agcaggtctt tgatttggct
     1081 gatgttggtg cttctgccga gaaccagtgc ggcgagtgcg gctttgcgcg gctgcgcgtg
     1141 aggacgacgc agggcggtac tgattttttg atagtggcgc agcgcggcaa aggcagtttc
     1201 ggcgatttcg tggcggtctt ggcggccgag ttttttgtgt tcgcggaaat aggcggagag
     1261 gacggcatcg gcaggctgtt tgaaagtcag catttcagcc aaaactttgg cggtatggtc
     1321 gagttgtgcg gcgttcatca ttaatgggtt ctcttaaagt ggggaaaggg tttcagacgg
     1381 ccttcataat gcgttcgatt tcgtgccaaa agccgtccgg ccgacattcc aaaacggcaa
     1441 tcaagccttg gtcgatacgg cggatttcgg ttgcgttcaa tgtttcggtt tcggcgacac
     1501 gcgcaggcgc gaggtaggcc atacggtcga gcagatggta gcggacttct tggcgtttaa
     1561 acccgtattc cgcccaatct tgaatataga tgccgcgcca accgtatgga ttaagcggtg
     1621 gctcaaaagc atggaaaccg tgtggaaaaa atccgatgcc gcgcacgatg gaaacgggtt
     1681 ttacgtcaag cggcagaccg tgttgccgca aggttcggat gccgtctgaa gtgtgcggca
     1741 gaccgagttg ggcggtcagt ttggcggctt ttcccaacag cgtgtctttg gggtcgagtc
     1801 cgcgcatgtc ttcgagcctg cccgtgccgc tgccgtaata tttgcaggtc agctcgatat
     1861 ggtagggctt gccgttaagc cttaccacaa aatccgccgc gccttgcgta ttgccgtccg
     1921 aaccggaaac cgtgaggttg tgcgcgagca gttcggcgtg tggcgcgttg gcaaaccaaa
     1981 atgccagcag ttcttcggcg taaatgccga ggcggtgtcc gagcggggcg cgtcgggtga
     2041 ggtgttccgt cagcggcgcg ggatcggcat ccaatgccaa aagatagcgg aaaccgcgtt
     2101 cccccaatag ttcgcgcacg cccaattcgc aaccgctttg ccacaaaggc ggcgcagtca
     2161 gcagcgaggc gaggtcgcgg acgggttggc tggtgagctt ccaccataat gcgtctaggg
     2221 cgtaattcat gcttatccga atatcagtca tgccgtctga aaagctggaa atgttttcag
     2281 acggccttaa tcagtctttt ttatggatac gatggctgac ggtattgaaa aaaccgcgca
     2341 ggatatcgat gtcttcggtt tgcgtgttgg cgcgtccgaa caggctctgc atacggcgca
     2401 tcaggcgttc accattgcgg cggttgaaaa agccgatgtc gttcatcacg ctttccatgt
     2461 gggcgaccat gcctttgatt tgctcgtggg tcgcagcgtg gtcttcttgt tgaagatggg
     2521 tcatgggcga accggtttgg ctgaagattt cgtagcacac gacctgcacg gcttgggcga
     2581 ggttgagcga gaaatagtcg ggattgccgt tgatggtcat cagtcggttg caggcttgga
     2641 cttcttcgat gctcaagccg aaagtctcgt tgccgaaaac cagtgccact ttctcgcctc
     2701 ggtttgcggt ctgcagtaat tcggatacca aatcgcgcgg ggtttgcagc ggcgcagtaa
     2761 tttcgcggcg gcggctggtc agggcgcagg cgatggtggt gtcggcaagg gcttcgtcca
     2821 aagaagcggc aatggtggca ttttccaaaa cgtctgccgc gccggaagcg aggatgaagc
     2881 tttcttccgg taatttaaac gattgaggat gctccgggtc aaacacgggc gggttttccg
     2941 tcatcggcgt tgccatcaga tttggggcga cgatggtcag tttgtgcaga cccattgttt
     3001 tcatcgcgcg cgcggcagag ccgatgttgg cgggatggct ggtgcgcgtg aggatgatgc
     3061 ggatgttgtc cagataagcg ggcagggcgg gtttgagagt agtcatgttc ggttttcggt
     3121 cggaatggaa tagggtataa tgcgccgtct ttttcgttcg gacggtcaaa tgccgtctga
     3181 aatgttcttt aacaacgtcg gaaatttaac gttgcgcctg aagcagacac gcgtcaggcg
     3241 gtattttacc cgatttggca ggatttaccc caaccaaata aactccgatt ttgccgctcc
     3301 gaaggactgt acgtccgaac cggcggtatg ccacccttaa ggaaatctga tgaatccgtt
     3361 tttgaataca gcctttaaag ccgcccgccg tgccggtcag atgatgatac gcgccgcggg
     3421 caatctcgat gccgtcaaaa ccgacagcaa agccttcaac gattttgttt ccgatgttga
     3481 ccgcaattcc gaaatcatcc tggttgaggc tttgaaagaa gcctatccgc accacaaaat
     3541 cacttgtgaa gaaagcggct tccacggcaa agccgcagcc gagtacgaat ggattatcga
     3601 tccgctcgac ggcacgacca atttccttca cggtcatccc caatacgcca tctctatggc
     3661 gctgctgcac aaaggcgtgt tgcaagaagc tttggtttac gcccccgaac gcaacgacgt
     3721 atatatggct tcgcgcggca aaggcgcgtt gctcaacgac cgccgcatcc gcgtttccaa
     3781 ccgcatcgaa ttgaaccgct gcctgatcgg taccggcttc cctgttgtcg atcaaagcat
     3841 gatggacaag tatctggtga ttttgaaaga tttcttggcc aaaaccgccg gcggccgtcg
     3901 tgaaggcgcg gcttctttgg atttgtgtgc tgtagcaaca ggtcgttttg acggcttttt
     3961 cgagtttaac ctcaaaccgt gggacattgc cgcaggtgca ttgattgttc aggaagcggg
     4021 cggtatcgta accgatatgt cgggtgaaga cggctggctg gaaagcggcg atattgtggc
     4081 tgccaatccc aaagtgctgg cgcaaatgtt gaaaatcatt tccgcacacg tttaaatttc
     4141 agcccgctcc cgggaatgcg ggaaaacggg cgggtatgcc gtctgaagta tcgcgccgat
     4201 atttcagacg gcatttttac tggcagcggg ggcaggaagg tggaaacggc taaatgcccg
     4261 aaactgccga ggatggaaac taaacggaga accctgtgca atttgtttat agtggattaa
     4321 caaaaaccag tacggtgttg cctcgcctta gctcaaagag aacgattctc taaggtgctg
     4381 aagcaccaag tgaatgggca aagccaacgc ctttaccgcg ccgattaccg tcgtcaatta
     4441 cgacgaacaa gccctaaaca acaccgaagc gcgtactttg gtggatgccg tagcgaaaaa
     4501 agacgcttcc gtttggcagt tcggcggcga aagcaacacg ctgaccggac tgtatttcag
     4561 aggttatcag cttgactcgc gccaattcag cgtcaacggt ttggcaggta tgtacggcac
     4621 gcaaggcacg gccagcgtgc aagttggatc ggcacaactg attaaaggcg catccactgc
     4681 cgtaaacggc atggaccctg aaggtgcggt atccggttcc gtcaatatcg aaaccaaaaa
     4741 agccgccgat gaaggcaacc gcaaaatcgg tttgggctgg ttcagtaaca accgcgccca
     4801 aggtacgttc gacttgggcc aacgtttcgg cgaaaacaaa gcattcggcg tgcgcgccaa
     4861 cggcaaactg cgccacggcg acaccccgcg ccacggttac agcgaagaca acaaagaatt
     4921 tgcggtaaat gccgactatc gcggcgaaac actgcgcgta gcgttcgatt ccatctacgc
     4981 aaaacgcaaa accaacggcg gtcgcgcgcg tatgcaggac attcaaaacg ccggcggacg
     5041 cttgtttggc gcgcccgatg gtaaaaccaa cctgctgcct tcttggaact ggcaaaatac
     5101 cgccggccaa accaatatgc ttacgtttga atgggatgcg tttgataatg cacagattac
     5161 aggcggtatc ggctacaaca aggcacgcta ttacggcacg ctgatttccc cgaccgtatg
     5221 tttgaatgct accagcactt gtaccgatat gcaaaacaca aacgccagcg gcaaagtaac
     5281 ggcacgcaac tatgactaca ataccggtac tgcgcgcctg accgaccaat acttccgcac
     5341 tttaagcatg aacctgtctg cgcgaggcga atttgaaacc ggcccggtaa cacataactg
     5401 gagtacggct ttcgaccgcg ttatccgcca gcggaaaacc attcgcggaa cggcagccgg
     5461 cgctggcaag gtagaagtga aggcaaacga aaatattgcg aatcaattgg catcgttcaa
     5521 agccgactat cccaattctt gggagaactc tgccaacttg gatgccaata tcaaagtcaa
     5581 cagcctggct ttgtccgaca ccttgggctt cgtggataac aaataccgcc tgactttggg
     5641 cggacgtttc caagcggttg aatacaccaa taagaaaaaa tcacagagcg gcgatgccaa
     5701 acgcttcagc ccgatgctga tggcggcgtg ggtgccgcaa cctgatttgg ttgtgtacgg
     5761 caactatatg gaagacttgg agcctgccga tatcaaaacc gatgattccg gcgaaacgac
     5821 aatggcgaaa ccgcgtgtca gccgtcagtt tgaagtgggc gtgcgtaaaa actggggcaa
     5881 tttcgtaacc acgttaaacg cgttccaaat caaacgcccg ggctactggc gcggtcatac
     5941 aactaccaag acaacaaaag gggtaacaac aacgctgacc tatggcaaca attccgattt
     6001 cgcccgctat aaggcgcaag gcggcgcggc cggcgacgag caaggtatgg aacgcaatcg
     6061 cggtattgag ttcaacacct acgcgaactt gctcaacaat actttgcgtc cgactttggg
     6121 tctgatgtat ctgcaatcga ccgtgaaaga gtatccaaat tcacgcgata tgctggttaa
     6181 cggcgtacaa gtcgccaatc cgcgcgtaat tgccaaagca ggtttggaat gggatacacc
     6241 gtttgccaaa ggattgactt taaacggtaa tgtttcgtat ttcggcaagt cttaccaaga
     6301 cacgcaaaaa caatacgcct tcccgtccta caccttgatt gacgtaggcg cgcgctacaa
     6361 aaccaagctc ggcaaaaata ctttgaccgt cagcagctcg gtggaaaacc tgttcaacaa
     6421 aaactattgg caggtacagc gcggccaata cgaccgcagc ttcgccgtcg tcggtatgcc
     6481 gcgcacttac tggctgaagg cggaattgga tttctgattt ccctatatcc ggaaaatgcc
     6541 gtccgaacag gtttcagacg gcatttcttt taaagagata tggcgggata cgcttaaata
     6601 atgctattct agcgtctttg aaacctgctt gtttgaagtg atgaaaaaac ctttgatttc
     6661 ggttgcggca gcattgctcg gcgttgcttt gggcacgcct tattatttgg gtgtcaaagc
     6721 cgaagaaagc ttgacgcagc agcaaaaaat attgcaggaa gcgggcttct tgaccgtcga
     6781 atcgcaccaa tatgagcgcg gctggtttac ctctacggaa acgacggtca tccgcttgaa
     6841 accggagttg ctgaataatg cccggaaata cctgccggac aacctgaaaa cagtgttgga
     6901 acagccggtt acgctggtaa accatatcac gcacggccct ttcgccggcg gattcggcac
     6961 gcaggcgtac attgaaaccg agttcaaata cgcgcctgaa acggaaaaag ttctggaacg
     7021 cttttttgga aaacaagtcc cggtttccct tgccaatacc gtttatttta acggcagcgg
     7081 taaaatggaa gtcagtgttc ccgccttcga ttatgaagag ctgtcgggca tcaggctgca
     7141 ctgggaagga ttgacgggaa aaacggttta tcaaaaaggt ttcaaaagct accgcaacag
     7201 ctatgatgcg cccttgttca aaatcaagct ggcagacaaa ggcgatgccg cgtttgaaaa
     7261 agcgcatttc gattcggaaa cttcagacgg catcaatccg cttgctttgg gcagcagcaa
     7321 tctgaccttg gaaaaattct ccctagaatg gaaagagggt gtcgattaca acgtcaagtt
     7381 aaacgaactg gtcaatcttg ttaccgattt gcagattggc gcgtttatca atcccaacgg
     7441 cagcatcgca ccttccaaaa tcgaagtcgg caaactggct ttttcaacca agaccgggga
     7501 atcaggcgcg tttatcaaca gtgaagggca gttccgtttt ggcacactgg tttacggcga
     7561 tgaaaaatac ggcccgctgg atattcatat cgctgccgaa cacctcgatg cttctgcctt
     7621 aaccgtattg aaacgcaagt ttgcacaaat ttctgccaaa aaaatgactg aggaacaaat
     7681 ccgcaatgat ttgattgccg ccgtcaaagg cgaggcttct ggactgttca ccaacaatcc
     7741 cgtattggac attaaaactt ttcgtttcac gctgccgtca ggaaaaatcg atgtgggcgg
     7801 aaaaatcatg tttaaagatt tgaagaagga agatttgaac caattgggtt tgatgctgaa
     7861 gaaaaccgag gcagacatca gaatgagtat tcctcaaaaa atgttggaag acttggcggt
     7921 cagtcaggca ggcaatattt tcagcgtcaa tgccgaagat gaggcggaag gcagggcaag
     7981 tcttgacgac atcaacgaga ccttgcgcct gatggtggac agtacggttc agagtatggc
     8041 aagggaaaaa tatctgactt tgaacggcga ccagattgat actgccattt ctctgaaaaa
     8101 caatcagttg aaattgaacg gtaaaacgtt gcaaaacgaa ccggagccgg attttgatga
     8161 aggcggtatg gtttcagagc cgcagcagta atgggtcaaa tgccgtctga aatgttcagg
     8221 cggcattttg ttgtattgga aactgccgtt ttcaaccatt cctttagata agccgtgtcg
     8281 gattcgtttt tatagtggat taacaaaaac cagtacagcg ttggctcgcc ttagctcaaa
     8341 gagaacgatt ctctaaggtg ctcaagcacc aagtgaatcg gttccgtact atttgtactg
     8401 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctataggccg gaggaaatat
     8461 tttaatccgc tgtaaaatag aaaatgccgt ctgaacattt cagacggcat ttgctcaaat
     8521 aaaccttatc ggctgtttat tcagtcggat ttattttttc ttttgcggag gcaggtcggt
     8581 acaagtaccc aatgccactt ccgccgccat accgatggat tcgcctaagg tcgggtgcgg
     8641 gtggatggtt ttgccgatgt ctgccgcgtc gcagcccatt tcgatggcaa ggcagacttc
     8701 gccgatcatg tcgccaccgt tcggaccgac aatgccgccg ccgatgatgc ggccggtttc
     8761 ggcatcaaaa atcagcttgg taaagccgtt gtcgcaaccg ttggcaatcg cacggccgga
     8821 agccgcccac gggaagttgg ctttggtgat tttgcggccg gatgctttgg cggacaactc
     8881 ggtttcgccc acccacgcca cttcggggga agtgtaggca acgcccggaa tcacgcgcgc
     8941 gtcgaagtag gctttgtggc cggcgcagtt ttcagcagca acgtggcctt cgtgaacggc
     9001 tttgtgtgcc aacattggtt gaccgacgat gtcgccgatg gcgtagatgt gcggcacatt
     9061 ggtacgcatt tgtttgtcca cttcgatgaa gccgcgatcg gtaacggcaa cgcctgcttt
     9121 ttccgcgctg atgagtttgc cgttgggcgc gcggccggcg gcaaccaata cggcatcgta
     9181 acgttgcggc tctttaggcg catttgcacc ttcaaaggta acgtaaacgc cgtcttcttt
     9241 cggctcgact gcaacggttt tggtgttgac cataatgttg tcaaaacggt attcgttttg
     9301 tttttgccat actttcacca agtcgcggtc tgcgccttgc atcaggccgt ccatcatttc
     9361 aaccacatcc aaacgcgaac ccagcgtgct gtaaaccgta cccatctcga ggccgataat
     9421 gccgccgccg ataatcagca gtttgcccgg tacttctttc agagccaatg cgccgctgga
     9481 atcgatgatg cgcggatctt caggaatgaa aggcagtttg gttacgcggc tgcctgctgc
     9541 aatgatacag tttttgaagg caacgatttt tttctcgccg gtagtggctg cctgttcgta
     9601 cgcgtcgccg gcagtcagcg acacttccaa gtggtgcggg tctaagaatt gcccgtcgcc
     9661 ttggataacg tccactttac ggcttttcgc catacctgcc aaaccgcccg tgaggcggga
     9721 aactacgccg tctttgtagg cgcgaagcat atcgatgtcg agttccggct cggggtattt
     9781 gataccgttg gcagccaagt ggcgcacttc gtcgataacg gcggcattgt gcaacaaggc
     9841 tttggaaggg atacagccga cgttcaggca aacgccgccc aaagttttgt aacgttcgac
     9901 gatggcgact ttcaagcctt catcggcagc ggcaaatgca gcggagtaac cgccgggacc
     9961 gccacccaat acgaccacgt cgtactcggc atcggcagaa ccgccgaatt gcgcggcttg
    10021 cggagcagga gcagcagctt taggggcttc ttgcgccggg gcggcagccg cttcggcttt
    10081 aggagcggca gccgtgcctt cagcttcaac gacaacaatc aaaccgcctt cggagatttt
    10141 gtcgccgact ttgactttca cttctttgac cacgcccgca acttcggcag gcacgtccat
    10201 cgtcgctttg tcggtttcca aggtaatcag ggtgtcgtca acggcgatgg tgtcgcccac
    10261 gtttacttca accgcgataa tatctacatt ttcgtgtccg ccaatgtcgg gcactttcaa
    10321 ttcaactaag ctcatgtctc atctttctga atgatgttca aacttccttt tcagacggca
    10381 cagaccgcta tgccgtctga agccgatcgg gttacagggt aatgcggcgg aagtctttca
    10441 acagcttcgc caagaatacg gtaaagcgca taccggccgc accgtcgatg acgcggtggt
    10501 cgaaggacag gctcaacggg cacatcaggc gcggggcaaa ctctttgccg ttccaaatcg
    10561 gtttgatttg ggatttgcac acgcccaaga tggcgacttc gggagcgttt acaatcggcg
    10621 tgaagcctgt gccgccgata ccgcccaagc tggaaatggt aaagcacgcg ccttgcattt
    10681 cttgcggttt gagcttgcct tcgcgggctt ttttggacaa ttcggtcagc tcttggctga
    10741 tttgtttcaa gcctttttga tccacgtctt tgatgacggg aacaaccaag ccgttcggcg
    10801 tatcggctgc gaaaccgatg ttgaagtagt ttttcagcac caggttgtcg ccgtccagag
    10861 aagcgttgaa ttcggggaag gctttcagcg cggaaacgga ggctttgatg atgaacgcca
    10921 acggtgacag tttcacgcct tcgcgttccc attctttgtt cagttgtttg cggaattctt
    10981 ccagctcggt catatctgct tcttcgtgta cggtaacgtg gggaatcacc acccagttgc
    11041 gagacaggtt ttgaccggag attttcttaa tgcgggacaa ttctttaact tcgacattgc
    11101 cgaatttgga gaagtccact ttaggccacg gcagcaagtc cagactgctg cccaaagatg
    11161 cgccggctgc ggcaggtttt gccgcgccac cctgcattac ggatttcaca aaggctttga
    11221 tgtcgtcgcc cacgatacgg cctttcaagc cggtaccttt gacttggccc aaatccacgc
    11281 ccaattcgcg cgccagtttg cgtgcggaag ggccggcgtg tgctttggcg aaagcggcct
    11341 cgtcgatttt ggcagcggca ggtgcagaag gtgcaggcgc ggcggcgggt gcggcggcag
    11401 cagtcggagg cggagcggca gccggtgcgg cagcttgagc ggcttgagca ggggctgctg
    11461 cggcagagcc ggcggtttct acttcgataa tggcagtacc ttcggacact ttgtcgccga
    11521 cttttaagaa tacggcttta acgacaccgg cagcggtaca aggtacgtcc atcgttgctt
    11581 tgtcggtttc caaagtaatc agcgtgtcgt cttcggcaac ggtatcgccg actttgatct
    11641 cgacggcgat tacatccaca tcggtatggc cgccgatgtc gggaacggct acttgaacgg
    11701 ttgcaccgcc tgcggcaacg ggtgcggcag caggtgcagg ttgtgcttcg gcagcagcag
    11761 gagcggcttc ggcagcggcg gcaccggttt caacggtcaa aattacgccg ccttcggaga
    11821 ttttgtcgcc gactttgact ttaacttctt tcacgacacc ggccgcatcg gcaggcacat
    11881 ccatcgtggc tttgtcggtt tccaaagtaa tcagggtgtc gtcaacggcg atggtgtcgc
    11941 ccgctttaac ttctacggcg atgatgtcga cgttttcgtg accgccgata tcggggactt
    12001 tgatttctac gatactcatt tgagttcctt taatcttttt cagttggatg ccgtctgaaa
    12061 actgcttcac gcggcatcgg tacggtttgt ctgttcaaac cattcgggca ttcggttttc
    12121 agacggcatg atgtctgaat ggggcaaaca ggccgtctga aacatcaatc agcgtttcca
    12181 gctaggggct gaatcggctt tgatgccgta tttttcaatg gcttgttgaa cagtttcttt
    12241 gctgactttg ccttgttccg ccaatgcggc caatgctgct acggcaacgt tgtagcgatc
    12301 cacttcaaag aagcggcgca ggttggcgcg actgtcggaa cggccgaaac cgtcagtgcc
    12361 caagacgtgg tagtcgttag ggatgtaggc acggatgcgg tcagcatagc tgcggatata
    12421 gtcggtagcg gcgataaccg gaccgtcatg accttgcagt tgagaggtaa cgaaaggcac
    12481 tttttcagct tccagcggat gcaggcggtt gaagcgttcg gtttcgatgg cgtcgcggtg
    12541 cagcaggttg aaggacgggc aagaccagat gtctgcttcc acgccgaagt cggctttcag
    12601 cagctcggca ccggcaatca cttcttgcag aatcgtaccg gaacccatca gttggacttt
    12661 cttgtcgcct ttgccgccgg ctttcagcag gtacatacct ttcaagatgt cttgttccgc
    12721 accttcgggc atatccggat gggtgtagtt ctcgttcatc agggtgatgt agtagaacac
    12781 gtcttcatta ttggcataca tacggcgcag accgtcttgt acgatgacgg cgacttcgta
    12841 ttggaaagtc gggtcataag atacgcagtt cggaatcagg tcggcctgga tgtggctgtg
    12901 gccgtcttcg tgttgcaggc cttcgccgtt cagcgtcgta cggccggcag taccgcccag
    12961 caggaagccg cgcgcgtgca tatcgcccgc cgcccaagcc aagtcgccga tacgttggaa
    13021 accgaacata gaatagtaaa tgtagaacgg aatcatggcg aagttgctgt tggcgtagct
    13081 ggttgcagcc gcaatccagt cggccatcgc gcccggttcg ttaatacctt cttgcaagat
    13141 ttgaccgtca acggattctt tatagaacat cagttggtct ttgtcttgag gggtatattg
    13201 ctgacctttc ggattccaaa taccgtattg gcggaacata ccttccatgc cgaaagtacg
    13261 gctttcgtcg ggaacgatag gtacgacgcg tttgccgatt tttttgtctt tcagcaaagt
    13321 ggacaggatg cggacgaatg ccatcgtggt cgagaactcg cgttcaccgc tggatttgag
    13381 ttgtgcgtcg aatgctgaca gctcgggcac ttccaatact tcctgcgtcg gtttgcgttg
    13441 cggcaggtag ccgcccaaag catcgcggcg tgcgtgcagg tatttgtatt cttccgtatc
    13501 gggggcaaaa gtcaggtaag gcagatcgcc gctttcgatt tgttcgtcgg taaccggaat
    13561 gtcaaagcgg tcgcggaatt gtttcaggga cgctttgtcc atttttttgg cttggtgggc
    13621 aacgttctga ccttcgccgg atgcgcccat accgtaacct ttaatggttt tcgccaagat
    13681 gacggtaggt ttgccgtcgg catggttcgc tgcgcggtcg taggcgttgt acactttttg
    13741 cgggtcgtgg ccgccgcggt tcaatgccca gagttgctca tcggtcatat cggcaaccaa
    13801 tgctttcagt tcgggcgtat tgaagaagtg ttcgcgcaca tacgcgccgt ctttggattt
    13861 gtaagtttgg tagtcgccgt ccaaacattc ttccatacgt tggcgcagga taccgtcttt
    13921 gtctttcgcc aagaggcggt cccagcggcg gccccaaatg actttgacga cattccagcc
    13981 ggcgccggca aagttgcctt ccaattcttg gatgattttg ccgttgccgc gtaccggacc
    14041 gtccaagcgt tgcagattgc agttgatgac gaaaatcagg ttgtccaagc cttcgcgtgc
    14101 agccagtgcg attgcacctt gagattcggg ttcgtccatt tcgccgtcgc cgcagaaaca
    14161 ccatacttta cggcctttgg tttttgccaa accacgcgat tccaagtatt tcaggaaacg
    14221 cgcctgataa atcgccatga tgggccccaa gcccatggat acggtcggga actgccaaaa
    14281 gtcgggcaag aggtgggggt gcggatagga aggcagaccg tgtccgtcca cttcttggcg
    14341 gaagttgttc agctggtctt cggtcagacg gccctcgacg aatgcgcgtg catagatgcc
    14401 cggggcgacg tgaccttgga agaagaccaa atcgccttct tcgccttcgc ctttggcttt
    14461 ccaaaagtgg ttgaaaccga cttcgtacat ggtggcggca gattggaaag atgcgatgtg
    14521 cccacccagt tccaaatctt tcttgccggc gcgcaatacg atggcggcgg cgttccagcg
    14581 cacgaatgcg cggatgcggt gttcgatgtt ttggtcgccc ggaatgcctt tttcgttttc
    14641 aaccgaaacg gtattcaaat acggtgtggt cgtgccgtgt ggcatacgta cgcccttgtc
    14701 gcggcagtat ttgaccaggt tttccaagag gtattgcgcg cgttcgccgc cttcatattc
    14761 gaggacggag cttaacgcgt ccagccactc ttgggtttcg atagggtcaa catcgtgtaa
    14821 ttgggtggac atagtatcta tcctttatgt tgagtgttta atcggcaggg agggtgtagc
    14881 caaccccttt attttcgttt gtgaaaaata ataagcaaaa acgaaaattt ctaaatcaaa
    14941 ccgaaaaaaa taatttaagt ttaatgtttt caaaaaatta taaaagtttt ggaaacaagg
    15001 gaaaacttga aaaaaatgtt cgatctgctc cacacgaaat cctatcagca atcgggtctg
    15061 ctggcaaata accatgcctg taaaagcagg ttttatactg aagttggcgt ttggtaaggt
    15121 caagtctttt tgatttcgaa atattctgtt tgattttggc gatatttttt cggaattgtg
    15181 gtatatcgaa tcattatgtc tctgtcggca attttcctat tgtagaaaat ctatacacaa
    15241 gctttgtgga taaccgtgtt gataagccac ggcttgtgtt aaaaaagtct tacgcaacaa
    15301 atgtacgctt attttgatta aaaaataggc acatttaaat ctataaaaat catgtgctta
    15361 tgttcaatat gatgtttctg gtcatgtttt tgccgtctgt tatgggattt ctcccgcgct
    15421 tggggcaggg ggtgaatttt ggaggttggt gcaaaacctg cttttttcgc gttacaatct
    15481 gcacccgatt tacttttgaa tttgcctgtt tggaaaaata cttttatgaa agccagccaa
    15541 ttctttatct ctactttaaa agaagcccct gccgaagccg cgcttgccag ccacaagctg
    15601 atgattcgcg ccggtctgat taaagccaac gcgtccggcc tttatacttg gatgccgatg
    15661 gggctgcgcg tgttacgcaa agtcgaaaac gtcgtgcgcg aggaaatggc gcgcgcgggc
    15721 agcgtggagc tgctgatgcc ggtggtgcag cctgccgagc tgtggcagga atccggccgc
    15781 tgggaatttt acggtaaaga actgctgcgc ctgaaagacc gccacgaccg cgatttctgc
    15841 atgggcccga cctgcgagga agtcatcgcc gacatcgtgc gcaaagaaat caacagctac
    15901 aaacagctgc cgaaaaactt ttaccacatc caaaccaaat tccgcgacga agtgcgcccg
    15961 cgtttcggcg tgatgcgcgc gcgcgagttc gtcatgaaag acgcttattc cttccatgcc
    16021 gactacgcct cgcttcagac gacctatgat gccatgtatg actcttactg ccgcatcttc
    16081 acccgtctgg gcttggcatt ccgccccgtc gccgcagaca ccggcagcat cggcggtacg
    16141 ggttcgcacg agtttcaagt gttggcggaa agcggcgaag atgttattgc gtacagcgac
    16201 acttccgatt acgccgccaa tatcgagttg gcaccgacct tgccgcttaa aggcgaacgc
    16261 gccgctgctc aggctgtgtt gaccaaagta catacaccaa acgtcaaaac cattgagtct
    16321 ttggttgaat tcctgaatat tccggttgaa caaaccctca aatccatcgt ggtcgaaggc
    16381 gaaaacgaag gcgaaatcgt cttattactg ttgcgcggcg accatgagtt caacgacatc
    16441 aaggcagaaa aactcgcagg cgtgagatcg ccattgacga tggcaagtcc tgccgcgatt
    16501 gtcgaacagt tcggcgcaaa cggcggctcg ctgggtccgg tcagctttgc aggtaaagtt
    16561 tatgccgatt ttgctactga aaaaggagcg gactgggtca tcggtgcgaa cgaagacggc
    16621 taccactaca ccagcttcaa cttcgggcgc gatgccgccg agcctgaatt cgtcgatttg
    16681 cgtaatgtgg tcgaaggcga cgaaagcccc gacgggcaag gccgtctgaa actcgcgcgc
    16741 ggcattgaag tcggacacgt cttccagttg cgcgacaaat atactaaagc catgaacgtc
    16801 agcttcctcg acaacaacgg caaatcgcaa attatggaaa tgggctgcta cggcatcggc
    16861 atcacccgcg tcgttgccgc cgccatcgag cagaataacg acgaaaaagg catcatctgg
    16921 accaaagcaa tggcgccgtt tgaagtcgtt atcgtgccga tgaactacaa aaaatcagac
    16981 accgtacgcg aagccgccga caaaatctat gccgaattgc tggcggcagg cgcggatgtg
    17041 ctgctggatg accgcgacga acgcgcgggc gtattgctga acgactccga gctgttgggc
    17101 atcccgcacc gcatcgtcat cggcgaccgc gccttgaaag aaggcaatgt cgaatacgcc
    17161 gaacgccgca acaacgaagc gcaggcagtt gcaatcggag aaattgttgc gcgtgtaaca
    17221 gcttcattaa atgcgtaagc ttggtcaaat gctgtctgaa aataatttca gacggcattt
    17281 ttatttgggg agaaagtgaa gtggcttcag tatttttaga aggagaagca gtcgaggtcg
    17341 gtaatatttt ctgtatcggc aggaactatg ccgcccatat cgaagagtta aaaaacgaaa
    17401 taccttccga acctgtggtg tttatgaagc cgtcaggcag cattttgaat agcggcggta
    17461 caatcttgct gcccgagttt agtagggatg tgcagtttga gtgcgaactc gtgttgctgg
    17521 tcggtaagga ttcagacggc atgggcgagg gtaaagatat tttgggatgt gtggcggggt
    17581 acggcgtggg tttggatttg accgcgcgag acatccaatg ccgtctgaag gcgcaggggc
    17641 tgccctggct gaaggcaaaa gggttcaggc attccgcgtg cgtgtcggat tttgcggcgg
    17701 caggcagaat cggcaatccc gagaagatgt tgttttcatt aaaacaaaac ggcgtattga
    17761 aacagcgcgg ggataccggt ttgatgattt acccgattcg ggaaatttta cacaaactgg
    17821 cggcagatta cggtttgggg aaaggggatt tggtgtttac cggaacgccc tccggcgtgg
    17881 gcgcaatcgg tgcgggggac aatttggcat tggagctgga cggcttggtg cacgcctctt
    17941 tcacggtagg gtgttaaccg cgccgcttaa ccctcacgcc cgttgaaaca ttccgccgga
    18001 ccgccctcga aaaaaccgtg caagatggct tgcgccgcca cttggtcgag caccgatttg
    18061 cgttttttgc cgaagacctg tgcttccgaa agcaggcttt cggcatagac ggacgacagc
    18121 cgttcgtcca cccaatagac ggggagattg aacctgccgt tcagcctgcg tccgaacttg
    18181 cgcgacaggt gcgtcatttc atgtttcgtg ccgtcggtat gcacgggcag tccgacgaca
    18241 aaataacgcg gctgccattc ttgaaccagc ttggcgattg ccgcgaactt ttcatcgttg
    18301 ctgccgccgg taacggtgct caaaggatgg gatagcccta attccgcgtc tccttgtgcc
    18361 acgccgatac gcgtttcgcc gaagtcgaat gccaatgctg taccttttgg ggctttatgc
    18421 atgtcccgcc tttccgtgca aagccagggg gtcgatgccg agtttggcga atgcggcggc
    18481 gtaacggtgt tcgtagggga tgtcgaacag gatgtgttcg tcggcgggaa cagtcagcca
    18541 cgcattgtcg gcaagttcgc gttcgagctg gccttttccc cagcttgaat agcctatgct
    18601 gatcaaggct ttgtcaaccg caccttcgcg tgaaatattt tcaatcacgt ctcgggaaga
    18661 agttagcgcg atattgtctg aaacgccgat actgctttgc cagttgccga tcggggtatg
    18721 cacgacataa cctcgatcga cttgcaccgg cccgcccatc atcacgctgt cgtgctgcat
    18781 ccgcatgggg atgtttttgc cggtggcgga aaaaatcatg tccatcgtaa tcggagaggg
    18841 tttgttgatg gcgatgccga gtgcgccgtc ttcatcgtgt ttgcagatat agacgaccga
    18901 ttgtgaaaaa aacgcgtctt ccatatcggg catggcgacg agaaagtggt tggagaggtt
    18961 catatcagcg tttgtcggga ttttctatcg ggttggaaat catgcaggcg ccggcaagca
    19021 ggttgccgga agtatcggct tgcaccccat cggtttgcgg tatgccgccg ccgaaacagg
    19081 aaaccgcgct taaggaattt ttcggcgagc cttggatgat tttatcgctg tacaccaaat
    19141 aggcgaaggt tttgcgtttg gggtcgtaat aacggacaat ctgccggctc ttgaacgcga
    19201 agctcgcacc gtgtttgaaa acttctttcg gtttgcgcac ggcggtttcg tcaaaagaaa
    19261 tcgaagatgc cgtctgaacg cacgaaaccg atgcgtcgga cgcgtcctct tccaaattga
    19321 ccatttcctt caagccgcct ttttttgcat acgaaatata acaggcaacc ccttgaacgt
    19381 cgggatcgtc gaatccttcc acttcgatac ggtcgttttt gcccagtatg ttgaaaacgg
    19441 tacttgcccg tccgatttta tcggtttcgc cgctgccgca ggcagtcagc aggacggcgg
    19501 cagacagcag tagcagtctg ttcatcaaaa tttccttatc ggttgaatcc ccgttttccg
    19561 tgaaggcggc agggcttgct ttgtttggag gggcgcacgt tcccaaaatc gggcaacata
    19621 cggtatgcgg cttaatgtgc taccctgtgt gtcggaacac aatatccgtt gcggcattga
    19681 aaatatgacg cacaatataa ggctgtttga gccgattgca agtatccgcg caaagaaggc
    19741 gcaagattgg gcaaacggca ttttcagacg gcataactga cagtataatc caaacgtccg
    19801 gctgctttcg tgcagccttc agtattatcc aactttttcc gaaggtcgaa ccatgcgcta
    19861 caaacccctt ctgcttgccc tgatgctcgt tttttccacg cccgccgttg ccgcccacga
    19921 cgcggcacac aaccgttccg ccgaagtgaa aaaacagacg aagaacaaaa aagaacagcc
    19981 cgaagcggcg gaaggcaaaa aagaaaaagg caaaaatggc gcagtgaaag ataaaaaaac
    20041 aggcggcaaa gaggcggcaa aagagggcaa agagtccaaa aaaaccgcca aaaaccgcaa
    20101 agaagcagag aaggaggcga catccaggca gtctgcgcgc aaaggacgcg aaggggataa
    20161 gaaatcgaag gcggaacaca aaaaggcaca tggcaagtcc gtgtccggat ccaaagaaaa
    20221 aaacgcaaaa acacagcctg aaaacaaaca aggcaaaaaa gaggcaaaag gacagggcaa
    20281 tccgcgcaag ggcggcaagg cggaaaaaga cactgtttct gcaaataaaa aagtccgttc
    20341 cgacaagaac ggcaaagcag tgaaacagga caaaaaatac agggaagaga aaaatgccaa
    20401 aaccgattcc gacgaattga aagccgccgt tgccgctgcc accaatgatg tcgaaaacaa
    20461 aaaagccctg ctcaaacaaa gcgaaggaat gctgcttcat gtcagcaatt ccctcaaaca
    20521 gcttcaggaa gagcgtatcc gccaagagcg tatccgccaa gagcgtatcc gtcaggcgcg
    20581 cggcaacctt gcttccgtca acctcaaaca gcgcgaggct tgggacaagt tccaaaaact
    20641 caataccgag ctgaaccgtt tgaaaacgga agtcgccgct acgaaagcgc agatttcccg
    20701 tttcgtatcg gggaactata aaaacagcca gccgaatgcg gttgccctgt tcctgaaaaa
    20761 cgccgaaccg ggtcagaaaa accgcttttt gcgttatacg cgttatgtaa acgcctccaa
    20821 tcgggaagtt gtcaaggatt tggaaaaaca gcagaaggct ttggcggtac aagagcagaa
    20881 aatcaacaat gagcttgccc gtttgaagaa aattcaggca aacgtgcaat ctctgctgaa
    20941 aaaacagggt gtaaccgatg cggcggaaca gacggaaagc cgcagacaga atgccaaaat
    21001 cgccaaagat gcccgaaaac tgctggaaca gaaagggaac gagcagcagc tgaacaagct
    21061 cttgagcaat ttggagaaga aaaaggccga acaccgcatt caggatgcgg aagcaaaaag
    21121 aaaattggct gaagccagac tggcggcagc cgaaaaagcc agaaaagaag cggcgcagca
    21181 gaaggctgaa gcacgacgtg cggaaatgtc caacctgacc gccgaagaca ggaacatcca
    21241 agcgccttcg gttatgggta tcggcagtgc cgacggtttc agccgcatgc aaggacgttt
    21301 gaaaaaaccg gttgacggtg tgccgaccgg acttttcggg cagaaccgga gcggcggcga
    21361 tgtttggaaa ggcgtgttct attccactgc accggcaacg gttgaaagca ttgcgccggg
    21421 aacggtaagc tatgcggacg agttggacgg ctacggcaaa gtggtcgtgg tcgatcacgg
    21481 cgagaactac atcagcatct atgccggttt gagcgaaatt tccgtcggca agggttatat
    21541 ggtcgcggca ggaagcaaaa tcggctcgag cgggtcgctg ccggacgggg aagaggggct
    21601 ttacctgcaa atacgttatc aaggtcaggt attgaaccct tcgagctgga tacgttgatc
    21661 ggattgccga aacggtaggc tttatgcaaa tcgtgccgat atggtgcgct gttccgtgaa
    21721 gtttgccgtt tgccgtacct gtattttccg gtttttggaa atcaagagag aaagttaaag
    21781 aagaatgtcg aaacctgttt ttaagaaaat cgcactttat actttgggtg caatcagcgg
    21841 cgtggccgtc agtctggcgg tgcagggttt tgccgccgag aaggacaggc gggataacga
    21901 agtcctgccg gtgcaatcca tccgcacaat ggcggaggtt tacggtcaaa tcaaggcaaa
    21961 ctactatcag gacaaacccg atgccgattt gtttgaaggt gcgatgaagg gtatggtggc
    22021 cggtttggat ccgcattccg aatatatgga taaaaaaggt tatgccgaga taaaggagtc
    22081 caccagcggc gaatttggcg gcttggggat ggaaatcggg caggaagacg gatttgtcaa
    22141 agtggtttcg ccgattgagg acacgcctgc ggaacgggcg ggggtgaaaa gcggcgattt
    22201 cattgtgaaa atcgataatg tttcgacacg cggcatgacg gtcagcgaag cggtgaagaa
    22261 aatgcggggc aagccgggta cgaagattac tttgacgctg tcgcgcaaaa atgccgacaa
    22321 gccgatagtc gtcaacctga cccgtgccat tattaaagtg aaaagcgtcc gccatcacct
    22381 gatcgaaccc gattacggct atatccgcgt gtcgcagttc caagagcgga cggtcgaaag
    22441 cgtcaatacc gccgcaaaag agctggtaaa ggaaaataaa ggaaaaccgc tcaaggggct
    22501 ggtgttggat ttgcgcgacg accccggcgg gcttttgact ggcgcggtcg gcgtgtcggc
    22561 ggcatttctg ccgtctgaag cagtcgtcgt cagcaccaag ggacgcgacg gcaaagaccg
    22621 catggtactg aaagccattc ctgaagatta tgtgtacggg atgggcggcg attcgttggc
    22681 gggcattcct gccgagttga aaaccatacc gatgacggta ttggtcaatt ccggttcggc
    22741 ttccgcgtcg gagattgtcg caggtgcatt gcaggatcat aaacgcgcgg tcatcgtcgg
    22801 tacgcagagc ttcggcaaag gttcggttca gactttgatt cctttgtcca acggcagcgc
    22861 ggtcaagctg acaacggcac tgtattatac gccgaacgac cgttctattc aggcgcaggg
    22921 gattgttccc gatgtcgaag taaaagataa ggaacgcatt tttgaaagcc gcgaggcgga
    22981 tttggtcgga cacatcggca atcctttggg cggcgaggat gtgaacagtg aaacccttgc
    23041 cgtgccgctt gaaaaagatg cggataagcc cgctgtaaaa gaaaaaggta aaaagaaaaa
    23101 ggacgaggat ttgtcttcaa ggcggattcc caaccctgcc aaagacgacc agttgcggaa
    23161 agctttggat ttggtcaagt cgcccgagca gtggcagaag tctttggggc tggcggcgaa
    23221 aaagccggtt tcaaataaag ataagaaaga taaaaaagat aagaagtagg tttttacgat
    23281 gccgtctgaa aggatttcag acggcatatt atgtttgaaa ggacaggtta tgagcgattt
    23341 tttaccggaa tttgaaccgt gcggcattta tagtgggtta acaaaaatca ggacaaggcg
    23401 acgaagccgc agacagtaca aatagtacgg caaggcgagg caacgccgta ctggtttttg
    23461 ttaatccact atacttgggt agcgattggg tttcagacgg catacattca ggatttcacc
    23521 gccttatgcc gtctgaaaga cgtttccgac ccatccctac tacacgcttt gtccgcaaac
    23581 gcatcacagg caagattatg gaagtcattc gataccccga ttcccccttc aaactccacc
    23641 aacccttcca gcccgcaggc gaccagccta ccgctattgc cgacttgctc gaagggcttt
    23701 cagacggcct tgcctaccaa accctgctcg gcgtaaccgg ttcgggcaaa acctacacca
    23761 tggcgaacgt catcgcccaa agcggccgac ccgccatcat catggcgcac aacaaaaccc
    23821 ttgccgccca gctttatgcc gaaatgcgcg agtttttccc tgaaaacgcg gtggaatatt
    23881 tcgtctccta ctacgactat taccaacccg aagcctatgt gccaagccgc gatttgttca
    23941 tcgaaaaaga cagtgcgatc aacgaacaca tcgagcagat gcgcctttcc gccaccaaaa
    24001 acctaatgac gcgcaacgac gtgattatcg tcgccaccgt gtccgccatt tacggtatcg
    24061 gcgacccgac cgagtatcaa caaatggtgt tgtccgtcaa agaaggcgat accatcgagc
    24121 agcgcgacat catcgccacg ctcgtttcca tgcaatacga acgcggcgat ttggacttca
    24181 aacgcggcag cttccgcgtg cgcggcgacg tgattgacgt gtaccccgcc gaaagctccg
    24241 aaaacgcctt acgcatcagc ctgtttgatg acgaaatcga ccgcctcgat atgttcgacc
    24301 cgctttcagg cagccttatc cagcgcgtcg gccgctacac cgtcttcccg tccagccact
    24361 acgttacccc gcgcgacacc gtcttgcgcg cctgcgagtc catcaaagaa gaattacgcg
    24421 aacgcatcga atttttcgcc cgcgaacaac gccccgtcga acaacaacgc atcgaacagc
    24481 gcacccgttt cgacctcgaa atgctctacg aaatgggctt ctgcaaagga atcgaaaact
    24541 actcccgcca cttttccggc aaaaaagaag gcgaaccgcc gcctacgctg atggactacc
    24601 tgcccgacaa cgccatcatg ttcatcgacg aaagccacgt taccgtaacc caaatcggcg
    24661 gcatgtacaa aggcgacgca tcgcgcaagc aaaacctcgt cgactacggc ttccgcctgc
    24721 cttcagcccg cgacaaccgc ccgctcaaat tccacgaatt tgaaaaagtc atgccgcaaa
    24781 ccatcttcgt ttccgctacc cccgccaaat acgaagaaga acacgccgga caagtggtcg
    24841 aacaagtcgt ccgtcccaca gggctggtcg acccccaaat catcatccgc cccgtcgcca
    24901 cccaagttga tgacttaatg agcgaaatca acgaccgcat ccaaaaaggc gaacgcatcc
    24961 tcgttaccac cctcaccaaa cgtatggcgg agcaactcac cgactattac agtgaactcg
    25021 gcatcaaagt gcgctacctg cacagcgaca tcgataccgt cgagcgcgtt gaaatcatta
    25081 gagatttgcg gctcggcctg tttgacgtac tcgtcggcat caacctcttg cgcgaaggcc
    25141 tcgacatccc cgaagtctcc cttgttgcca tcctcgacgc cgacaaagaa ggcttcctgc
    25201 gctcccaccg cagcctgatt caaaccatag gccgcgccgc gcgcaacgtg aacggcgtcg
    25261 cgattctgta cgccgacaaa atcaccgact ctatgaaagc cgccatcgac gaaaccgaac
    25321 gccgccgtga aaaacaaatt aaattcaacg aagaacacgg catcgtgccg caacagatta
    25381 aaaaacaggt caaagacatc attgacggcg tgtaccacga agaagacagc ggcaaaggcc
    25441 gtagacaagg caaaaacaaa gttaaagtcg gcgaaattca caacgaagaa gacgcgatta
    25501 aagaaatcgc caaattggaa aaagccatgc aacaggcagc tagggatttg cagtttgaag
    25561 aagcgtcggt gttaagggat aggattcggg ggattaggga agggttgtta tttgggagtg
    25621 agtaataaaa gcacttgaaa gtttagttga tgaccacaat gttcttaaaa gatttggaga
    25681 aacaaaagat gtggagaaac atcgtaactt gatcaattac attctgagga ctgaaaatga
    25741 catattcttt tcgtgtagaa aacataaatg atatatctga tattcctgaa aatcatttgg
    25801 aaatagttag agctgctctg cctagtaata gaagtaatgc cttatctcgt gagccagatg
    25861 gggcacaagc tgtttttgtt tatgaaattg atggtgaatt tagagctaag ttatttgacc
    25921 gcataaaaga tacagacagt tatttacaag caacagagga taattactct aatgaccctc
    25981 gcagtataga agtagtatac gttttggagc aacagcaagg gtagcctgag ggtcgcacaa
    26041 actctcaagc tgacgtgcag tatgtgtgat acgcacgcat gcgttttttc gttcaaacca
    26101 ttgttttaaa ccttgtatcg aaacatcact cgctgcttta tcttgaaaaa atgccgtctg
    26161 taagcaaaca tgcttcagac ggcatttttt caaacagcgg tttgttatct gatccgtttg
    26221 aataccaagt cccaaacgcc gtgtccgaga cgtttgccgc gcgcttcgaa tttggtttcg
    26281 gggcggtagt ccggggtagg ggcgtagtct gtcgccgtat tttgcaggct gtcgaagcta
    26341 ctgaggactt caagcatctg ctgtgcatat tcttcccagt ctgtcgccag gtggatatag
    26401 ccgccggttt tgagtttggg cagtagtttg gcgatgaagg gggcttgtat cagacggcgt
    26461 ttgtggtggc gttttttgtg ccacgggtcg gggaagaata tgtggatgcc gtcgagcgag
    26521 ccgtcttgca gcatattttc gacaacttct acggcatcgt gccgcatcac gcggatgttt
    26581 tctaaatggt tttcgttgat gagtttgagc aggttgccta cgccgggatc gtgtacgtcg
    26641 atggcgagga agtcggtttc gggcaggcgg agggcgattt ctgcggttgc tgtccccata
    26701 ccgaagccta tttcgaggat tttggggcgg ttgcttccga aattggcatc aagatcgacc
    26761 ggtgttgctt ggtaatcgat gccgaagctg tcccataagg tatcgatggc gcgttgctgc
    26821 gcggcggtca tatggctttg gcggaggacg aaactgcgga tgctgcgggg atgtccggct
    26881 tgacggtcgt ttggtgtttg gttttcggcg gtgtctgtca taatgcttcc gtgcgcttgt
    26941 gcaatcagga tgccgtctga atgtgaaagg cctgttcgga cggcgggaag ggaaaggggc
    27001 gattatacct gtttcgtttc ggcagggtag gtgtaacgct gttcagacgg cattttgcca
    27061 ttgttgcagc agttcttgca ggacgctttt ttgcccgtat ccgttgttta tggcttcttg
    27121 caggtagcgg caggcttttt ctttgtccgg cccgacaccg tgtccgctgt aatggatgca
    27181 ggcgaggttg tagaacgcca tactgtcttt ttttgcggcg gctttttcaa accatttgcg
    27241 tgcttcattg tagtcggcgg tcataccctg tccgaagtaa tacatgctgc ccagattggt
    27301 ttgggctttg ctgtgtccga gttctgcggc ttctgtgtag agtttgagcg cggctgcgta
    27361 ttcgggcgcg cattgtaatc cgtaatgcag ggcttgggct ttttgataaa gctgttctgc
    27421 cctttgccgc ctaatggcgg aatctttaat gccgccgtat tggtcaggat ggtttaacgc
    27481 gctgtcggat atgagtttct gataggcggt aaggctgccg gcttcggctg cctgccggta
    27541 ataatgccgg gctttttctt tatcggaagt catgcccagt ccgtagcggc agatgtcgcc
    27601 cagtatccgc aaaccttcgg gatggcgttc ggcggcggct gcttcggcgt gatgcagggc
    27661 aagtttgtag tttctttccg gatgcttgcc ttgcaggtag atgtcggcaa gtgcggcatg
    27721 ggcatcgggg tcattttcct gtgcggcctg ttgaaaccgg taaacggctt tatcgggagc
    27781 cgtaggtagg atgagctcgg caagcggggt gtaggcggga atgtatcctt gttgtgcggc
    27841 ggcgcgcaaa tgattgatag cttgggtcgt gtcggttggt gtaccttgtc cgaaacggta
    27901 ttgaaggccg agctgccatt gtgccgccgc caagccttgt gctgcagcct cgcggtagag
    27961 aaggtgtgcc gtttcaaaat ccggcggcgt gccgtaccgg cagtattggg caagcaggta
    28021 ttgcccgtgg gggttgttgt tttttgcgct tgtttttgcc catttcaggg cttcttcaaa
    28081 atgtcgggac gcatacagca ggtacatcag gcggacttgt gcttcaggca gcccttcctc
    28141 tgctaatggt ctgtaaagcg atatcagggt ttccgggggt gcgccttcca attcttggat
    28201 atatgccaga ttgtaacgcg cgtagggatg tccctgttcg gcggcgatgc ggagcatttc
    28261 cataccttgt tcgcggtctt ggtaggggct gtccgcgtcc atcaggtgtt ttgccaactc
    28321 gaatgcggcg gcgggatgac ctgccgaggc cgcttctgtc agcagtcggc ggacttcggt
    28381 aaagttatat tgtttttgag acaggcgttc taaggcctgc cgatagggta agtcgggatt
    28441 gttcatatca gatcgttttc cgttttgtcg aatcatcgtt attgtgttat gaagttaggt
    28501 ttatag
//
LOCUS       DAWFIE010000018        28401 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWFIE010000018 DAWFIE010000000
VERSION     DAWFIE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28401
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.018"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            356..1660
                     /locus_tag="WGQ71_000739"
     CDS             356..1660
                     /locus_tag="WGQ71_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ0706789.1"
                     /translation="MKKHIWAASLLPTSLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
     gene            complement(1905..3200)
                     /locus_tag="WGQ71_000740"
     CDS             complement(1905..3200)
                     /locus_tag="WGQ71_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0706790.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAIRSQHWEELQQWHEHAQKYYGATHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGTKIFEHSPMIRIEP
                     YQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFKPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(3326..4213)
                     /locus_tag="WGQ71_000741"
     CDS             complement(3326..4213)
                     /locus_tag="WGQ71_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0706791.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGMLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(4213..5178)
                     /locus_tag="WGQ71_000742"
     CDS             complement(4213..5178)
                     /locus_tag="WGQ71_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0706792.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNIAISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(5194..6318)
                     /gene="potA"
                     /locus_tag="WGQ71_000743"
     CDS             complement(5194..6318)
                     /gene="potA"
                     /locus_tag="WGQ71_000743"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0706793.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(6513..6620)
                     /locus_tag="WGQ71_000744"
                     /pseudo
     CDS             complement(6513..6620)
                     /locus_tag="WGQ71_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6699..6968)
                     /gene="rpsO"
                     /locus_tag="WGQ71_000745"
     CDS             complement(6699..6968)
                     /gene="rpsO"
                     /locus_tag="WGQ71_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ0706794.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(7191..8126)
                     /gene="secF"
                     /locus_tag="WGQ71_000746"
     CDS             complement(7191..8126)
                     /gene="secF"
                     /locus_tag="WGQ71_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ0706795.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(8130..9983)
                     /gene="secD"
                     /locus_tag="WGQ71_000747"
     CDS             complement(8130..9983)
                     /gene="secD"
                     /locus_tag="WGQ71_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ0706796.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALQNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(10195..10461)
                     /gene="yajC"
                     /locus_tag="WGQ71_000748"
     CDS             complement(10195..10461)
                     /gene="yajC"
                     /locus_tag="WGQ71_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ0706797.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            10589..11698
                     /locus_tag="WGQ71_000749"
     CDS             10589..11698
                     /locus_tag="WGQ71_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ0706798.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACTGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALAHTSKYLNRLESRLPEDGKICRL
                     DDDTVISKNSLSAARFSAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGAMYALAEYRLNRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRAFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRYDI"
     gene            12061..13125
                     /locus_tag="WGQ71_000750"
     CDS             12061..13125
                     /locus_tag="WGQ71_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ0706799.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIVHD
                     DVPTGSGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            13266..13589
                     /locus_tag="WGQ71_000751"
     CDS             13266..13589
                     /locus_tag="WGQ71_000751"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ0706800.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            13661..13984
                     /locus_tag="WGQ71_000752"
     CDS             13661..13984
                     /locus_tag="WGQ71_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ0706801.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            14031..14234
                     /gene="tatA"
                     /locus_tag="WGQ71_000753"
     CDS             14031..14234
                     /gene="tatA"
                     /locus_tag="WGQ71_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ0706802.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            14238..14924
                     /gene="tatB"
                     /locus_tag="WGQ71_000754"
     CDS             14238..14924
                     /gene="tatB"
                     /locus_tag="WGQ71_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ0706803.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPFPDAANTLLDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            14938..15708
                     /gene="tatC"
                     /locus_tag="WGQ71_000755"
     CDS             14938..15708
                     /gene="tatC"
                     /locus_tag="WGQ71_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ0706804.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTEQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            15761..16369
                     /gene="maf"
                     /locus_tag="WGQ71_000756"
     CDS             15761..16369
                     /gene="maf"
                     /locus_tag="WGQ71_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ0706805.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYRVIQLPAGIDESVKAGETPFAY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPHSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPIYETVSMLQDLGYRTPLSALKP"
     gene            16378..16731
                     /locus_tag="WGQ71_000757"
     CDS             16378..16731
                     /locus_tag="WGQ71_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ0706806.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLAWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            16721..18535
                     /locus_tag="WGQ71_000758"
     CDS             16721..18535
                     /locus_tag="WGQ71_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ0706807.1"
                     /translation="MPAETTVSGARPAAKLPIYILPCFLWIGIVPFTFALRQQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACKSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPM
                     LQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPPALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     TIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            18725..19402
                     /locus_tag="WGQ71_000759"
     CDS             18725..19402
                     /locus_tag="WGQ71_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0706808.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            19416..20822
                     /locus_tag="WGQ71_000760"
     CDS             19416..20822
                     /locus_tag="WGQ71_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ0706809.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            20940..23366
                     /locus_tag="WGQ71_000761"
     CDS             20940..23366
                     /locus_tag="WGQ71_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ0706810.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EENQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFGSHPLYGDILTACCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYSSDFLLKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTIIPLLPEDAEAKQQFVRSLGPEARYTRFMTHTNELPTATLARLC
                     NPDYHCEAAWTARDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLHTDHKIT"
     gene            23411..23656
                     /gene="rpsP"
                     /locus_tag="WGQ71_000762"
     CDS             23411..23656
                     /gene="rpsP"
                     /locus_tag="WGQ71_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ0706811.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            23672..24181
                     /gene="rimM"
                     /locus_tag="WGQ71_000763"
     CDS             23672..24181
                     /gene="rimM"
                     /locus_tag="WGQ71_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ0706812.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            24181..24930
                     /gene="trmD"
                     /locus_tag="WGQ71_000764"
     CDS             24181..24930
                     /gene="trmD"
                     /locus_tag="WGQ71_000764"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ0706813.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRSLIPKESRLLETIRQEQREIQS"
     gene            24946..25311
                     /gene="rplS"
                     /locus_tag="WGQ71_000765"
     CDS             24946..25311
                     /gene="rplS"
                     /locus_tag="WGQ71_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ0706814.1"
                     /translation="MNLIQQLEQEEIVRLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            25633..26358
                     /locus_tag="WGQ71_000766"
     CDS             25633..26358
                     /locus_tag="WGQ71_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ0706815.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLSRQDIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPSTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            26393..27268
                     /locus_tag="WGQ71_000767"
     CDS             26393..27268
                     /locus_tag="WGQ71_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ0706816.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            27345..28259
                     /locus_tag="WGQ71_000768"
     CDS             27345..28259
                     /locus_tag="WGQ71_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ0706817.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSSDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         7273 a         8388 c         6653 g         6087 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggatcggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgcgccggtc tggcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc gacatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggta tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggcatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgcgta tttttaagcg ggatgcagat agacgctgcc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggcgcgcatt gggtctgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtcg ggcgcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggcgaacg cgtggaataa gggcggctat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga taaacatata
     1741 gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaaa tagtacggaa
     1801 ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgaggca
     1861 acgccgtact ggtttttgtt aatccactat actgcctgcg acgcttaacg gctgtctttc
     1921 cactgataat atttcgagcc gaggaaagaa ccgagtttgc gcaaaaacgg ttgcaggata
     1981 atattcggct ggcgcaaccg ctcaaacggc ttcagacggc attcgtcccc taaaattgct
     2041 tcggcaaccg ccagacctgc aatgcctgtt atcgccatcc cgtgtccgga ataaccttgc
     2101 gtataaaaaa cattcggggc taaacgtccg aaatgcggga caaggttggc ggtaatgtcg
     2161 cactccccgc cccacgaata ttcgattttg acatcggcaa gctgcggaaa aactttaagc
     2221 atatcttggc ggacaagctc ggtcatacgc gcgggattgt cgataaactc gttatcctta
     2281 ccgccgaaaa gcagtctgcc gtccgcgctg aggcggtaat aatccaaaat atggcggttg
     2341 tcgcatactg ccatattgtt gcggataagc ccttttgcac gcgcgcccaa gggttctgtg
     2401 gcaataataa aggtgctgac agcaatcgcc ttgcgctcca aaggcttgaa tatcggattc
     2461 aaaccggcat aagtattgac ggcgtacacc agatttttgc attcgacgct gccttcgggg
     2521 gtgtaaacca gccaaccgtt ttgataaggt tcaatgcgta tcatgggaga atgctcaaaa
     2581 atcttcgtac cagcttcggc agcggcgcgg gcgatgccca acgtgtaatt gagcggatgg
     2641 agatgcccgg acaagggatc gaactgtgcg ccttggtaca tatcgctgtc aagctgctgc
     2701 ttcagttcag tgttatccca gagttgataa tgagttgcac cgtaatattt ttgggcgtgc
     2761 tcatgccatt gttgcaattc ttcccaatgc tgcgaacgga tggcaaccgt ggcataaccg
     2821 cgctgccaat cgcaatcaat ggcatgtttg cggacgcgtt cgtccaccag ttcgaccgcc
     2881 tgcaaagact gttgccaaaa ccattgcgct tgctccaaac cgacctgttt ttcaatttcc
     2941 tccataccgc aggcgtaatc gctgataacc tgcccgccac tccgtcccga cgcgccgaaa
     3001 ccgatacgcg cggcttccaa cacaaccgtt tcatgtccct gctccgccaa gggcaatgca
     3061 gtgcacaaac cactcaatcc gccgccgatg atacaggtat cggttttcag acggcattga
     3121 agtttcggat aaacagtatg aggattaacc gaactgaaat aataagaagg cagatattct
     3181 tgaaaatcag ggcgaatcat tgtgtttgct ttatcgggta tattttcgga cggaatgata
     3241 caggctgtcg agccatatcg tccaaacaga aaatcggttg aagaaaacag gctgacccag
     3301 tcatgcggtc agcctgcctt attaattaat ttgctttctc ggcagccaat ttttcttggc
     3361 ggtaggcttc tgccgcttct cggtcacgct tggttgcctg cctcatcatc caataattga
     3421 cgatgatgac caatgttccg atgatgccga tcaggatggt tgccaaaacg ttcatctgcg
     3481 gatcaagacc cagcttgatt ttggagaaaa tcacctgcgg caatgtggat gaaccggggc
     3541 cggagaggaa tgaggtaatc accaaatcat ccaaagacag ggtaatgccg agcagaaagc
     3601 ctgaagcgat ggcaggggca atcaaaggca aagtgatgac aaaaaagatt ttcagcggac
     3661 gcgcgcccaa atccattgcg gcttcttcga gcgattggtc aagctcaacc agacgcgaac
     3721 ggataacaac ggtaatgtat gccatgcaca gcgtcgtatg tcctaagaaa atagtgaaaa
     3781 agccacgatc gaagtagaga tgttgtaacc attcgctgcc ctgcaaaaat atctgtacct
     3841 gaataatcag cagcagcata gacagaccgg taatcacgtc gggcatcacc ataggtgcgg
     3901 aaatcatacc ggcgaacaag gtactgccgc gaaaacgttt aatccgcgcc atcgcatagc
     3961 ccgccagcat gcccaaaacg acggcggcaa gcgaagacac aacggcaatc cgcagcgaca
     4021 gccaagcggc ttccaaaatg gtgtcgtttt ccaacaatgc gccgtaccac ttggtcgaaa
     4081 agccgcccca aacggttacc agcttggatt cgttaaacga atagatgacc aaaacgacca
     4141 gcgggatata cagaaacgcc agcgacagtg ccaacatcag tttcaagaac caagataatt
     4201 tggatttctg cattatttgg ctccttcttc caattcgcgg ttttcataat gctgaaacag
     4261 ggcaatcggc acgaccagca gcgcgaccat cacgacggcg acggcggaag ccagcggcca
     4321 gttgttttga tcgaagaacg cctgccacaa gactttacca atcatcaggt tttccgaacc
     4381 gccgaccagc tcgggaatga cgaactcgcc gacagcaggg acgaaaacca gcatggagcc
     4441 tgcaataatg ccggttttcg acaaaggcag ggtaatcgtc aagaacgatt tgaccggccc
     4501 cgcgcccaaa tcggaagccg cttcaagcag gcggttgtcg agtttcacca gttgcgtgta
     4561 tagcggcaga atcataaacg gcagataggc gtaaaccatc accaaattga gcgaaaaggc
     4621 attgtagaac aaatccaaag gctcgctgat aatacccatt ttaatcaaca ggttgtttac
     4681 aataccgtta tgcccgagca gacccatcca cgcatagacg cgcaacagga acgatgtcca
     4741 aaagggcagc ataatggcaa gcagcaaacc attgcggaca gaaggattgg cacgagaaat
     4801 cgcataggcg gtcggataac cgaccaacag gcaaatcact gtcgtcatca atgcggtttt
     4861 gattgaagac cagtaggtca tcagatagat attgctgttt tcactgtcgc cgaacgggtt
     4921 aagggtatgc caaaaatttt ggaaaatatc ggcatagttt tgataactga tggcgatatt
     4981 cagacgacct aaatcttcgt cgatgctggt cagcggcgta aacggcggaa tggcgatttc
     5041 ttgttcggca aagctgattt tcagcacgat ggcgaacgga atcagaaaca gcaccaaaag
     5101 ccaaatatac ggtacggcaa tcaccacacg ctgccccggg cggcggaaca gtttgttttt
     5161 cagtttatta aggttcattg cattcccctt aaatcaacgg aacaacggag tcggttggtt
     5221 ttccggccag ctgatataga cggtttcgtc ccaagttggc ggtgtaatgt tgcgcacata
     5281 ccagtaaggg gcggggactt ggcttttgac gacgcgcccg tttgcgagtt tgatatggta
     5341 tatggcgaag ctgcccaaat aggcaatttc ttttaccgtg cctttcgccc agttgtagtc
     5401 gcccaaatat tcgggttttt ctttatataa atcaatatcc tctggtcgaa tgctaaccca
     5461 aaggtcctgc tcgctcggac cacccaaacc gtgatcaatg cggacgtggt tttccaaacc
     5521 ttcgcattcg ataacggcat agtcggcatg atcttcaatc accacaccgt caaagatgtt
     5581 ggtttcgccg ataaactcgg cagtgaagcg gctgttggga tagtcgtaca cgtcgctggg
     5641 tgtgccgact tgctgcaact gaccgtcgga cataatggcg atgcgggtcg ccatcgtcat
     5701 cgcctcttct tggtcgtgtg taaccataat acaggttacg ccgacttgtt ccagcgtatt
     5761 gaccaactca agctgggttt gttggcgcag ttttttgtcc aatgcaccga ggggctcatc
     5821 cagcagtaga attttcggac gttttgccag actgcgtgcc aaagcaatgc gctgctgctg
     5881 accgccggac aattggtgcg gtttgcgttt agcaaatttg gtcatctgaa ccaggcggag
     5941 catttcttcg acgcgcgcgg cgatttcgcc tttaggcatt ttgtcctgtt tcagaccgaa
     6001 ggcaatgttt tgttctacgg tcatatgcgg aaaaagcgcg taactttgga acatcatatt
     6061 gatggggcga tcatagggtg caagtttggt aatatcctga ccatcaagga taatttttcc
     6121 ctgattggga ctttccatac ccgccagcat acgcagcagt gtagattttc cgctgccgga
     6181 actgcccaaa agggcgaaga tttcgtgttg ataaatgtcc aagtcgatgt tatcgacagc
     6241 gtaattgtca ccaaactttt tcaccaaacc ttggattttg agataaggtt tggctgaaga
     6301 cgcagtggtt gcggtcataa tggcaatact ccaataaaaa gacgagtacc ggcaaaacgg
     6361 attttcgaat gggtgataaa aagctgtttg attgctggcg ggagttaaac gtttgatgcc
     6421 gtctgaaact cttgtaaagc gcacgggcag catgaaatgg aacaagattc caaagaactt
     6481 tatattatat tagtttatgc ggttttcggg caatatagtg gattaaattt aaaccagtac
     6541 agcgttgcct tgccttgccg tactatttgt actgtctacg gcttcgtcgc cttgtcctga
     6601 tttaaattta atccactata aatatggatt tgagcttgtc ggaaagcaac agaaaagaaa
     6661 acaccgccca tttttctggg cggtgtcgga aagcgtaatt atttacgcag acccaagcgg
     6721 gtaatcaacg cgcgatacgt atcgggctgg gtacggcgca agtaggccag caggcggcgg
     6781 cgttggctga ccattttcaa caggccgcga cggctgtggt ggtctttggg gttggctttg
     6841 aagtgggggg tcaggtcgtt gatgcggaaa gtcaacagag cgacttgtac ttcggaagag
     6901 ccggtgtcgc cttctttgcg ttggaaatct ttaacgattt gtgctttttg ttctacggtc
     6961 agtgccataa tgaaaactcc aaaaatataa gaatccccat agggattcag acaagtttgc
     7021 caagcctgaa gacaacggca aactccctat gcccaagata ggacaacgtg gcattatgac
     7081 acaattcccg catttctgca caatatttta agaccgtgga agtaaccgtt aaaaatgccg
     7141 tctgaagcat tgtctgcttg agacggcaat gttcagacgg catatgcgct tcaaaccacg
     7201 atttcttctt tcttcttcgg ttctttaccg atattgtcgc ggctcaaacc gaacattagc
     7261 agaagcgggc tggcaaccaa tacggaagaa taaatgccga acacgatgcc aatggtcaac
     7321 gccatagaaa agccgtgcaa ggccgcaccg ccgaacacca gcatagaaac aaccatcgcc
     7381 tcggtcgaac cgtgggtaat gatggtgcgg ctcatcgttg cggtaatcgc gttgtcgatg
     7441 acttccggca cggcatgtcc gcgcatcgcc ggcttgcgga agttttcacg gatacggtcg
     7501 aagacgacga cggattcgtt cacagaatag cccaatacgg caaggatacc cgccaagacg
     7561 gtcagcgaaa attcccattg gaagaaggca aagcagccga gaataatcac gatgtcgtgc
     7621 atattggcga taatggcaga tacggcaaaa cgccattcaa aacgcatcga caggtaaata
     7681 atgatgccga taacgacaaa acctaaagcc atcaatccat tacttaccaa ttcctcaccg
     7741 acttgcgggc cgataaattc gacttggcgc aaggtaacgt cgggactgtc ttttttcagc
     7801 aaatccataa cctgattgga caactgtgcg gaagtaacac cttctttgtt cggcaggcgg
     7861 atcatgatgt gtttgttcgt acccaatgcc tgaacctgta catcacctat tttcagcgta
     7921 tcgaggcgtt cgcgcatctt attgacatcc gcaccctgct gatattggac ttccattacc
     7981 gtgccgccgg taaattcgac agagaaattc agacctctgg taaccaaaaa gaatacggcg
     8041 gcaataaagg taaccaacga aataaaggtc gtcagtttgc cgtagctcat aaacggaata
     8101 tcgcgtttga ttttaaagag ttccatagct tactccttgc ctcctgccat ttcagctttc
     8161 ggcttccaca ccgaaccgat ggaaatattc tgcaatttgc gtctgcgtcc gtaccacaga
     8221 ttgaccaacg cacggaatac gacgacggac gaatacatcg aagtcagaat acccaaacag
     8281 tgtacgaccg caaaaccgcg taccgggccg gaaccgaata ccaaaagcgc gataccggca
     8341 atcagcgaag tcaggttgga atcgacaatg gtcgcccatg cgtgttggaa accgagattg
     8401 attgcctgct gcggcggcac gccggcacgc aattcttcgc ggatacgttc gttaatcaag
     8461 acgttggagt cgattgccat acccaaagtc aacgccagcg cggccatacc cggtaacgtc
     8521 aacgttgcct gcatggcaga caaaataccg attaggaaca gtatgttggc actcaatgca
     8581 atggtagaaa agaaacccat cagacgatag taaaccacca tgaatgcagc aacgatggca
     8641 aaaccccata aagtcgaatg gaagcctttt tcgatgttct ccttacccaa agacggaccg
     8701 atggtacgtt cttcgacaat ctgcatcggt gcggcaagag aaccggcacg caacagcaaa
     8761 gacgtatcat tggcttcggc tgtcgtcatg cttccggaaa tttccacgcg tccgcctgta
     8821 atggcagtac ggataaccgg cgcggttaca acctcggatt ttccttggtc gatcaaaacc
     8881 atcgccatgc gtttgccgac atttgcggca gtcagttcgc cgaaaatgct gccgcccgcg
     8941 ctgtccaagc tcagactgac ggcaggtgcg cccatttggt cgaaactcgg ttgcgcatcg
     9001 ttgatgttgt cgcccgtcag ctcgacctgt ttgctgatta gcagagtttc gggacgatct
     9061 ccgccgcttg aaagcagctc ataaccgctc ggcacgttgc cttccaatgc ctcgcgcaac
     9121 ttggcaggat cgtcctccac catacgcaat tccaaagtcg cggtacggcc gatgatgtct
     9181 tttgccttgg cagtatcctg aacgcccgga agctgcacga cgatacggtc ggcacctgac
     9241 tgctggatga cgggttcggc cacgcccaac tcgttcacac ggttgtgcag ggtagtgatg
     9301 ttctgtttga ccgcatcgga acgcacttta ttgaccgcct cttccgaaag cgtcaagacg
     9361 atattgctgc cgtctgaatt cagcgttgct tcaggaaaca gcttgcgcaa ctgcggcagt
     9421 gccttttgca catcacctgc atcctgcaaa gggacggtca ggctgtttcc agcctgacgc
     9481 accgtgccgc tgcggatttt ttcgcggcgc agttcgcggc ggatgtcgcc cgaataacgt
     9541 tcaaacgttt tctgcatcgc cgctttcata tcgacctgca tggtgaaatg cacgccgccg
     9601 cgcaggtcca aacccaaaaa catcggattg gctttgattt tcgccatcca ttcggggctg
     9661 tccgccaaca ggttgagcgc ggtaatatac ccttcgccca aagtgttttc gatgacgtcg
     9721 cgcgctttaa gctgcgtttc tgtgtctttg aaacgcactt tcagtgaatt gtccacaaca
     9781 aacatcccgt cggtctgaat acccgcgttt tgcagcgcgg catccacttt ggattgagtc
     9841 tgttcgttga tgatgatggc ttgtcggttg gtcgatacct gcacggcggg tgtttcgccg
     9901 aataggttgg gcagcgaata cactgcggca accgcaatcg tgaacacaat cagcagatat
     9961 ttccataaag gataacggtt catcattgtt ccttaatggt tggaacccca ccctttcggt
    10021 ggtgtcggaa tcgggctatt tcagaagagg caaaaaccct tcccagccag gcaagaccgg
    10081 aaggcggcat cttgaatatg ccgccctgcg tgtcggaaca tggtcaagcc ttcggttgga
    10141 attcaaaaca aagtgccgca ttcgggcttt ccagatgcgg cttgtcggca caaatcaatc
    10201 gacttttgcg gcaatcgcat tgcgttctac ttcgacctcg atttttgtac cctgtccgat
    10261 atccacggta aaaaactgtt cgccgactct ggttacctta cccttgaaac ctgccgccaa
    10321 gaccactttg tcgccgactt tcaaggcggc aagcattgcc tgatgcgctt tgaatttctt
    10381 ttgctgcgga cgcatgatca ggaagtagaa caccaccata atcaacacta aaggagcaaa
    10441 ttgtgcaaca gcttgattca taatttatcc gttctttcta atatggttga aaatcgagag
    10501 gggtatataa taacataaga ccgtaaacaa tatatcgggt ttgccgtccg taccgaccgt
    10561 ataccgcagc ctgcccgtcc acaaacccat gtccttgacc cgcctaatct tgaaattcta
    10621 tgcgctgttg cgcctctttt tgggcaaaaa cgcccgcacc gcatggattt cgcatcccgc
    10681 ctgcaccggg cacgaacccg gcgcaaacca tcccgattcg cccgaccgca tcctctgcat
    10741 cgagcaggca ttgcgccgcg ccggtatttg gcagcacctc caaaccatag aggcggaaga
    10801 aatcagcgat acgcgcctcg cgcttgccca tacgagcaaa tatttaaacc ggttagagtc
    10861 ccgcctgccc gaagacggca agatttgccg cttggacgac gacaccgtta tcagcaaaaa
    10921 ctccctgtct gccgcccgtt tttccgcagg ttcggcagtt caggcagtcg acatggtcat
    10981 gaaccgtaaa gcatggcatg ccttttgcgc cgcccgcccg cccggacacc atgcgggcag
    11041 cggcaaagcc ggcggattct gcctgctgaa caacgttgcc gccggtgcga tgtacgccct
    11101 tgccgaatac cggctgaacc gcatcgccgt catcgatttt gatgtacact acggcgacgg
    11161 tacggcagaa atattcaaag acgatccgcg catcctgttt ttcaacctgt ttgaaaccga
    11221 ccttttcccc ttccccgaaa acaacgatat gcccgacggc ggcaatatgg tgcacctgcc
    11281 cttgccgccg ggaacgggca gccgcgcatt ccgcgaagcc gtccgcagac agtggctgcc
    11341 ccggcttgcc gcattcaaac ccgaactggt gctgctgtcg gcaggattcg acgcacaccg
    11401 tctggacgaa tcgggcaggc ttaacctgca cgaggcggat tttgcctggc tgacacacaa
    11461 aatcattcag acggcatcgg gctgccctgg caaaatcgta tccgtactcg aaggcggcta
    11521 cacccttgaa cctctggcac aatctgctgc cgagcatatc cgcgtgctgg cagggctggg
    11581 caaatccgat gccgcaaccg cctatcagaa aacactggac ccaacaaaaa aacggtttgc
    11641 caaaccgaaa accggacagg tgcgccaacc tacccaatcc gaccgttacg acatatagtg
    11701 gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacaaatag tacggaaccg
    11761 attcacttgg tgcttcagca cattagagaa tcgttctctt tgagctaagg cgaggcaacg
    11821 acgtactggt ttttgttcat ccaccataaa tgccatctga agccgattcg gttttcagac
    11881 gacatcatta tttgacctga cgcaaacaga aagccttccg cgtgcttaaa cacatctctt
    11941 gtagtaaaat atctaaacag attcaactgg aaacggtcag gcttcttcac gccattccca
    12001 tttcaaccgc cgcctgccag attcagacgg tattcctact tgaaaacaag gagcgttaca
    12061 atgaaagcag cacgttttta cgacaaaggc gacatccgca tcgaagacat ccccgaaccg
    12121 accgtcgccc ccggcaccgt cggcatcaat gttgcctggt gcggcatctg cggtaccgac
    12181 ctgcacgaat tcatggaagg cccgattttc attccgcctt gcggtcatcc gcacccgatt
    12241 tccggcgagt ccgcacccgt cacgatggga cacgagttct ccggcgtggt ctatgccgtc
    12301 ggcgaaggcg tggacgacat caaagtcggt caacacgtcg tggtcgagcc ttacatcgta
    12361 cacgacgacg taccgaccgg ctcgggcagc aactaccacc tctccaaaga catgaacttt
    12421 atcggcttgg gcggctgcgg cggcggcctg tccgaaaaaa tcgccgtcaa acgccgttgg
    12481 gtgcatccga tttccgacaa aatcccgttg gatcaagccg ctttgatcga accgctgtct
    12541 gtcggacacc acgcctatgt acgcagcggc gcgaaagcag gcgacgtcgc attggtcggc
    12601 ggtgcaggtc cgatcggttt gctgttggct gccgtgttga aagccaaagg catcaaagtc
    12661 atcatcaccg agttgagtaa agcacgcaaa gacaaagcgc gcgaatctgg cgttgccgac
    12721 tacatcctcg acccgtccga agtcgatgtt gtcgaagaag tgaaaaaact gaccaacggc
    12781 gaaggcgtgg acgtggcatt cgaatgtacc agcgtgaaca aagtgttgga tactttggtc
    12841 gaagcctgca aacctgccgc caatttggtt atcgtatcca tctggagcca ccctgcaacc
    12901 gtcaacgtcc acagcgtcgt gatgaaagag ctggatgtgc gcggcacgat tgcctactgt
    12961 aacgaccacg ccgaaaccat caaactggtc gaagaaggca aaatcaacct cgaacccttc
    13021 atcacccagc gcatcaagct ggacgaactg gtttccgaag gcttcgagcg tctgatccac
    13081 aacaacgaat ccgccgttaa aattattgtg agtccaaacc tgtaagcaaa ttacatttgc
    13141 aacagcacaa atgccgtctg aaacgctcag acggcatttt ccaacgaagc gcaacaggcg
    13201 tattgcccga gagcagccgt ttagcctatc attccgaaca aacagaaaca aacagaagga
    13261 aaatcatggg agattccgta ctatccgcca tccaacaaac catcacccag cgaaaatctg
    13321 ccaatccgtc cgaatcttac gtcgcacagc tcttgcataa gggcgaagac aaaatcctaa
    13381 aaaaagtgat tgaagaggcg ggcgaagtgt tgatggcatc caaagacaaa aacccgtccc
    13441 acctggttta cgaagttgcc gacttatggt ttcacaccat gattcttctg acacaccacg
    13501 acctgaaggc ggaagacgta ttggacgaac ttgcgcgccg ccaaggtttg tcgggcttgg
    13561 ccgaaaaagc cgctcgcaca gaatcttgaa tttatattaa aatccgcact ttcccacatt
    13621 caatccgtct gaccgctttt cagacggcat cggagccgtt atggacaact gtattttctg
    13681 caaaatcgcc gccaaagaca ttccggcgca aaccgtctac gaagacggcg aaatggtttg
    13741 tttcaaagac atcaaccccg ccgctccggt tcatctgctg ctgattccca aagtccattt
    13801 cgattcgttg gcacacgccg cacccgaaca tcagcccctt ttgggaaaaa tgatgctgaa
    13861 agttcccgaa atcgccaaag cggcaggact ggcagacggc ttcaaaaccc tgatcaatac
    13921 cggaaaaggc ggcggacaag aggtcttcca cctgcatata cacatcatgg gtacacccgt
    13981 ataaaccgtt atttcacaat caacccctaa tacttactta aggatacatc atgggcagtt
    14041 tttctctgac gcactggatt atcgtactga ttatcgtcgt tttgatattc ggcaccaaaa
    14101 aactgcgcaa cgtcggcaaa gacctcggcg gtgcggttca tgacttcaaa caggggctga
    14161 acgaaggcac agacggcaaa gaagcccaaa aagacgatgt aatcgaacac aaaaaagacg
    14221 aagacaaagc gtaatttatg tttgatttcg gtttgggcga gctggttttt gtcggcatta
    14281 tcgccctgat tgtcctcggc cccgaacgcc tgcccgaggc cgcccgcacc gccggacggc
    14341 tcatcggcag gctgcaacgc tttgtcggca gcgtcaaaca ggaatttgac acgcaaatcg
    14401 aactggaaga actaaggaag gccaagcagg aatttgaagc tgccgctgct caggttcgag
    14461 acagcctcaa agaaaccggt acggatatgg agggtaatct gcacgacatt tccgacggtc
    14521 tgaagccttg ggaaaaactg cccgaacagc gcacgcctgc tgatttcggt gtcgatgaaa
    14581 acggcaatcc ctttcccgat gcggcaaaca ccctattaga cggcatttcc gacgttatgc
    14641 cgtccgaacg ttcctacgct tccgccgaaa cccttgggga cagcgggcaa accggcagta
    14701 cagccgaacc cgcggaaacc gaccaagacc gcgcatggcg ggaatacctg actgcttctg
    14761 ccgccgcacc cgtcgtacag accgtcgaag tcagctatat cgatactgct gttgaaacgc
    14821 ctgttccgca taccacttcg ctgcgtaaac aggcaataag ccgcaaacgc gatttgcgtc
    14881 ccaaatcccg cgccaaacct aaattgcgcg tccgtaaatc ataaagagag caatccggtg
    14941 tccgaaacac aaaacgaaca acccgtccaa ccgcttgtcg agcatctcat cgagctgcgc
    15001 cgccgcctga tgtggacggt tgtcggcatc ttagtctgct ttttcggcct aatgccgttt
    15061 gcccaacaac tctatacttt tatcgccgac ccgctgatgg caaacctgcc caaagacacc
    15121 agcatgattg ccaccgatgt catcgcaccg tttttcgtgc cggtcaaagt taccctgatg
    15181 gcggcatttt taatttcgct gccgcatacg ctctaccaaa tctgggcatt tgtcgcgccc
    15241 gcactctacc aaaacgaaaa acgcctgatt acgccgctcg tcctctccag cgtcagcctg
    15301 tttttcatcg gcatggcatt tgcctacttt ttggttttcc ccgtcatttt caaattcctt
    15361 gccagcgtta cccctgtcgg tgtcaatatg gcgacagaca tcgacaaata cctctccttc
    15421 atcttgggga tgtttgtcgc attcggtaca acgtttgaag tccccattgt cgttatcctg
    15481 ttaaccaaaa ttggtgtggt aacaaccgaa cagctcaaac gcgcccgccc ctatgtgatt
    15541 gtcggcgcgt ttgtcattgc cgccatcatc acgccgcccg atgtgatttc acaaaccctg
    15601 cttgccattc cgctgattct cttatacgaa gcaggtattt ggttcggacg ctttttcacg
    15661 ccacgttcag aacaggatgg cgacatacag ccgcctgcaa caacctgaca ctatgccgtc
    15721 cgaacctccg cctcataccg ccacagatta aggaatacct ttgaataccc tctatttagg
    15781 ttcaaacagc ccgcgccgaa tggaaatcct gacgcagttg ggctaccgcg tcatccaact
    15841 gcctgccggc atcgacgaat ccgttaaagc cggcgaaaca cctttcgctt acgttcaaag
    15901 gatggcagaa gaaaaaaacc gaaccgccct gaccctcttt tgcgaaacca acggcacaat
    15961 gcccgatttc cccctgatta ccgccgacac ctgcgtcgtt tcagacggca ttatattagg
    16021 caaaccccac tcccaagccg aagcaatcga atttttaaac cgattgtccg gcaaacaaca
    16081 taccgtcctg actgctgtct gcattcatta tcgcggcaaa acgtcaagcc gcgtccaaac
    16141 caaccgcgtc gttttcaagc ccctgagttc ggaagaaatt tccgcctatg tccaaagcgg
    16201 cgaaccgatg gacaaagccg gcgcctacgc cgtacaaggc ataggcggca tctttatcca
    16261 atctatcgaa ggcagcttca gcggcattat ggggctgccc atttatgaaa ccgtttcgat
    16321 gttgcaggat ttgggatacc gtaccccctt gtccgccctt aaaccgtaaa gaccgccgtg
    16381 aacagacaaa ccgcttacct ccttacctct ttcagcctga tcgcactgat aatcctgtcc
    16441 cttgcttggg aactgtggat tgcaccgttg cgcccgggcg gctcgtggct cgcgctcaaa
    16501 gccctccccc tctgtctgcc gctttcaggc atcttgaaaa agaaaatcta tacttaccaa
    16561 tacagctcca tgctggttct gatttatttt gccgaagccg tcatgcgcct gttcgacgcc
    16621 tatccggcag aacaaatttg cgccgcgctt tccgcagtat tcagcatcat cttcttcata
    16681 tcatgcctgt cgttcgtcaa acaatacaag gaaacaaaca atgcccgctg aaacgaccgt
    16741 atccggtgcg cgccccgccg ccaaactgcc gatttacatc ctgccctgct tcctttggat
    16801 aggcatcgtc ccctttacct tcgcgctcag gcagcaaccg tcgcccgact tttaccacga
    16861 tgctgcagcc gcagccggcc tgattgtcct gttgttcctc acggcaggaa aaaagctgtt
    16921 tgatgtcaaa atcccaccca tcagcttcct tctgtttgca atggcggcgt tttggtatct
    16981 tcaggcacgc ctgatgaacc tgatttaccc cggtatgaac gacatcgtct cttggatttt
    17041 catcttgctc gccgtcagcg cgtgggcctg caagagtttg gtcgcacact acggacaaga
    17101 acgcatcgtg accctgtttg cctggtcgct gcttatcggc tccctgcttc aatcctgcat
    17161 cgtcgtcatc cagtttgccg gctgggaaga cacccccatg cttcaaaaca ttattgttta
    17221 cagcgggcaa ggcgtaatcg gacacatcgg acagcgcaac aacctcggac actacctgat
    17281 gtggggcata ctcgccgccg cctacctcaa cggacaacga aaaatcccgc ccgccctcgg
    17341 cgcaatctgc ctgattatgc agaccgccgt tttaggtttg gtcaattcgc gcaccatctt
    17401 gacctacata gccgccatcg ccctcatcct tcccttctgg tatttccgtt cggacaaatc
    17461 caacaggcgg acgatgctcg gcatagccgc agccgtattc cttaccgcgc tgttccaatt
    17521 ttccatgaac accattctag aaacctttac cggcatccgc tacgaaactg ccgtcgaacg
    17581 cgttgccaac ggcggtttca cagacttgcc gcgccaaatc gaatggaata aagcccttgc
    17641 cgccttccag tccgccccga tattcgggca cggctggaac agttttgccc aacaaacctt
    17701 cctcatcaat gccgaacagc acaccataca cgacaacctc ctcagcaact tgttcaccca
    17761 ttcccacaac atcgtcctcc aactccttgc agaaatgggg atcagcggca cgcttctggt
    17821 tgccgcaacc ctgctgacgg gcattgccgg gctgcttaaa cgccccctga cccccgcatc
    17881 acttttccta atatgcacgc ttgccgtcag catgtgccac agtatgctcg aatatccttt
    17941 gtggtatgtc tatttcctca tccctttcgg actgatgctc ttcctgtccc ccgcagaggc
    18001 ttcagacggc atcgccttca aaaaagccgc caatctcggc atactgaccg cctccgccgc
    18061 catattcgca ggattgttgc acttggactg gacatacacc cggctggtta acgccttttc
    18121 ccccgccact gacgacagtg ccaaaaccct caaccggaaa atcaacgagt tgcgctatat
    18181 ttccgcaaac agtccgatgc tgtcctttta tgccgacttc tccctcgtaa acttcgccct
    18241 gccggaatac cccgaaaccc agacttgggc ggaagaagca accctcaaat cactaaaata
    18301 ccgcccccac tccgccacct accgcatcgc cctctacctg atgcggcaag gcaaagttgc
    18361 agaagcaaaa caatggatgc gggcgacaca gtcctattac ccctacctga tgccccgata
    18421 cgccgacgaa atccgcaaac tgcccgtatg ggcaccgctg ctacccgaac tgctcaaaga
    18481 ctgcaaagcc ttcgccgccg cgcccggtca tccggaagca aaaccctgca aatgacccgc
    18541 gccggcggat gcggataccg cccgaaatgt aaagctccat gcaagacatt gcaaaaaaca
    18601 gcaaaccggt agggaaatac gctatcaaaa aacattgcag ccgtgttaag ataaaccgtc
    18661 aaacaatctt ttcacgcccc gcccgaaaca gggtcggggc atacccttac gaaaaggaaa
    18721 caccatgagc cgcgtattac tcgtagatga cgatgccctg ctgaccgaac tgctgaccga
    18781 atacctgagc gccgaaggtc tgaacgtccg cagcgttccc gacggggaag caggcgtaca
    18841 ggaaatcctg agcgggcagt acgatgtagt cgtattggat tccatgatgc ccaaaatgaa
    18901 cggcttggat gtcttgaaaa acgtacgcgc ccgaagcacc gtccccatca tcatgctgac
    18961 cgccaaaggc gacgacatcg accgaatcat cggcttggaa atgggcgcgg acgactatgt
    19021 ccccaaaccc tgcacaccac gcgaactctt ggcacgcatc aatgccatcc tgcgccgcgc
    19081 acaacacagc ggcgaacaga acaacgcacc caacagcatc tccgtcagcg atgtcgtcct
    19141 gtaccccgcc aaacgccagg catccgtcaa agacatgccg ctcgaactga ccagcaccga
    19201 attcaacctg ctcgaagtcc tgatgcgcca tgccggacag gtagtcagca aagaaaccct
    19261 gtccgtcgaa gcactcgacc gcaagctggc aaaattcgac cgcagtatcg acgtacacat
    19321 ctccagcatc cgccacaagt tgggcgatgc ctctctgatt caaaccgtac gcggcttggg
    19381 ctacctgttt gtcaaaaact gaaataaaca gataaatgaa actgttccaa cgcatcttcg
    19441 ccacattttg cgcggttatc gtctgtgcaa tctttgtggc gagtttttct ttctggctgg
    19501 tgcagaacac ccttgccgaa aaccagttca accaacgccg caccatcgaa accactttga
    19561 tgggcagcat catttccgca ttccgggcac gcggggacgc gggtgcgcgc gaaatcctga
    19621 cggaatggaa agacagcccc gtctcatcgg gcgtgtacgt tatacagggc gacgagaaaa
    19681 aagatatcct gaaccggtat atcgacagct acaccatcga acgcgcccgg cttttcgccg
    19741 ccggacaccc gcattccaac ctcgtccata tcgaatacga ccgcttcggc gaagaatacc
    19801 tgttcttcac caaagactgg gacaaactcc aagcccgccg cctgcccagc cccctgttga
    19861 tccccggtct gccgctcgcc ccgatttggc acgaactcat catattgtcc ttcatcatcg
    19921 tcgtcggact gctgatggcg tacatcctcg ccggcaatat cgccaaaccc atcagaatct
    19981 taggcaacgg catggacagg gtggcaaacg gagaacttga aacccgtatc tcccaacagg
    20041 tcgacgaccg cgacgacgaa ttgtcccatc ttgccatcca attcgacaaa atggtggaaa
    20101 aactcgaaaa actcgttgcc aaagaacgcc acctgctcca tcacgtctcc catgaaatgc
    20161 gttctcccct tgcgcgcatg caggcaattg tcggactgat tcaggcgcag ccccaaaaac
    20221 aggagcaata tctcaaacgg ctggaaggcg aactgacccg catggatacg ctggccgggg
    20281 aactgttaac cctgtcccgt ctcgaaactt ccaatatggc tttggaaaaa gaaagcctga
    20341 aactcctgcc cttcctgggc aacctggtag aagacaatca aagcattgcc cagaaaaacg
    20401 gacaaacggt taccctgtct gccgacggaa aaatccccga aaacacaacc atccttgcca
    20461 acgaaagcta cctgtaccgc gccttcgaca acgtcatccg caacgccgtc aactacagtc
    20521 ccgaaggcag caccatcctg atcaacatcg gacaagacca caaacactgg ataatcgacg
    20581 ttaccgacaa cggccccggc gtggacgaaa tgcagctccc gcacatcttc accgctttct
    20641 accgtgcaga ctccagtgcc aacaaacccg gaacaggact ggggcttgca ttgacccaac
    20701 atattattga acagcactgc ggcaaaatca tcgccgaaaa catcaaaccg aacgggctgc
    20761 ggatgcgctt catcctaccc aagaaaaaaa ccggttccaa aacagaaaaa agtgcgaact
    20821 gaccataatg ccgtctgaac cggcttcaga cggcattgca caaacagtta ccccaaaaca
    20881 aacacacagc gggtgcatct ccaaaaatca cgcacatacc gtccgcaaag gaatatatca
    20941 tgtcggcaca aaccgatccc ggctacttct tcatgcccaa ccacattatc ctgataggcg
    21001 cgagcgaacg tccgtacagc ctgggcgaac gcgtactcag caacctgctg agtacgccct
    21061 ttcaaggaaa aatcaccccc gtaaacccgc gccaccacac catagccggg cttcccgcct
    21121 acaccagcct caacaaaatc cccggcaatg cagacctgat tattgccgtt accctacccg
    21181 acagttacga caccctcttc aaaacctgcc gcaaaaagca gctccgacac atcatcctca
    21241 tacaggactg ggacagcctg tctgccgcag aactgcacac cgccgaaact gccatccgca
    21301 aacaccacgg caacggactc aacatcaccg cctgcaccac cgcaggcatc caactgccct
    21361 cactcggact taacatcagt acccaagacg gatacgccgc aggccatacc gccatactga
    21421 ccggcaatgc cgccgtcagc cgccaaatcg acaacatcct gaacaaactc cgtcaaggca
    21481 catcccgcca catcagcctg catcccggca tcagccccat cacatccgcc gattggctca
    21541 accgcttcgg acacagcctg cacaccaaaa ccgccgtcct acaccacaac cctgaagaga
    21601 atcagcgcaa actgttcagc gcaatccgcc aatttacccg ccatacgccg ctgattctcc
    21661 acatcacctg ccgcacgaca gaaaccgacc gtgccgtact gcactgcctc gcccgacact
    21721 gcaacttcct catcagtttc aacgccgacg acctcgaagc cgcactgcgc gcccaactgt
    21781 ccgaccttcc acccctgtcc cgactcgaca tcctgtccga cacgcctgcc gaatggctga
    21841 acgcgcacgc gccaaaaacc ctcgccctcc actttcccaa ccttccccac cacatccgca
    21901 acggacacct gaccggcaca cccacaccct caatctgcca cgacatcgcc tcacgtcagc
    21961 ttgcccgccc cgacacccaa gccgtcctga ccatcctcag tccctccgga cacgaggatt
    22021 acaaaaaaac agcacgcgcc cttatccgcc tgtccgaaca gaccgccaaa cccctgctcg
    22081 tcagcagccc cttttcagac ggcataaccc atttcgacac ccccactcag gcaatccgca
    22141 ccctttccta ccgcaacacc gccgccgccc tgaaacaggc acagctcgac attgcaccgc
    22201 cgcagccatg ccgtctgaaa acaccgcaac cccaaaacat caaaaaagcc cttgcagcgg
    22261 caaatccctc cctgctcgcc gaagccctgc acctcccccc ctaccggcac actacccata
    22321 acgccgtcca attccaattc ggcagccacc ccctctatgg cgacatcctg accgcatgct
    22381 gcaacggaca aaccactgcc gtactcccgc cgtttaccac gctcgacagc cgccaccttg
    22441 cccgctttgc cgaactcgac ggcacacaaa ccctcgacca gttcctgcac acactgaccg
    22501 tcattcccga ataccgccaa cacattctcg gcatcaccct caacctcaac ggcggacaat
    22561 acagcagcga tttcctctta aagacacccg aaacacacga cacgcccaaa cgcaagaaca
    22621 caggcaaagc cgcccaaacc ctcgaacatg ccgccgcaaa aatgcagagt gccgccgcat
    22681 acctgaaaca caaaaacccg acagccgccg aatttctccg ccacacaagc gaagccgccg
    22741 cagaactgct cggcagcaaa accgaaaccg gagcagccgt acccaacgta cttgccccct
    22801 atcccgcagc ataccccaaa acactgtccc taagaaacaa cacgaccgtt accattatcc
    22861 cccttttgcc cgaagacgca gaagccaaac agcagttcgt ccgcagcctc ggtcccgaag
    22921 cacggtacac acgcttcatg acccacacca acgaactgcc cacagccacg ttggcacgcc
    22981 tgtgcaaccc cgactaccac tgtgaagccg catggacggc aagggatgcc gacagcaaca
    23041 tcgtcgccgt cgtccgccac agccgcctga atcgcaacga atgcgaattc ggcatcacac
    23101 tggcggaaca tatgcgcggc agcgggctgg cacagaaaat gatggaactc atcatccaaa
    23161 ccgccgcaca gcaaggctac cggactatga gcgccgacat tctcaaaacc aataccccca
    23221 tgatcaaact tgctgaaaaa tcaggattta ccctcaagga atcggacacc gaaaaaaacc
    23281 tgtaccgcgc atatctgaac cttgcggcag acaaaacaac agaaaaaaca aataaaaact
    23341 tgcacaccga ccacaaaata acctaaaatc ggcagtttcc atatatccga ttttctcaaa
    23401 aggactcaaa atggtagtta tccgtttggc acgcggcggc tcgaaacacc gccccttcta
    23461 caacgtcatc gttactgact cacgcagccg ccgcgacggc cgcttcatcg aacgcgtagg
    23521 cttctacaac cccgtagcca acgaaaaaca agagcgcgtc cgcctcaatg cagaccgcct
    23581 gaaccactgg attgcacaag gcgcgcaagt cagcgactcc gttgcaaaac tgattaaaga
    23641 acaaaaagcc gcctaattca catttgccgc catgacagac actcaaaacc gggtagccat
    23701 gggctacatc aaaggcgtat tcggcataaa aggctggctg aaaattgccg ccaacaccga
    23761 atattccgac agccttttgg actaccccga gtggcatttg gtcaaggacg gcaaaaccat
    23821 cagcgttacc cttgaagccg gaaaagtcgt caacggcgaa ctccaagtca aattcgaagg
    23881 cataaacgac cgcgacttgg cattctcatt gcgcggttac accatcgaaa taccccgtga
    23941 agcattcgcc ccgacagaag aagacgaata ctactggaca gacttggtcg gcatgaccgt
    24001 cgtcaacaaa gaccataccg ttttaggcaa ggtaagcaac ctgatggaaa ccggcgcaaa
    24061 cgacgtattg atgattgacg gagaacacgg gcagattctg attccgttcg tttcccaata
    24121 tatcgaaacc gtcgataccg gcagcaagat tattactgcc gactggggtt tggactactg
    24181 atgcttatcc aggcagttac cattttcccc gaaatgttcg acagcattac ccgctacggc
    24241 gtaacgggac gcgcgaacag acagggaatt tggcagtttg aagcagtcaa tccccgaaag
    24301 tttgccgaca acagattggg ctacatcgac gaccgcccgt tcggcggcgg cccgggaatg
    24361 attatgatgg ctccgccgct tcatgcagca atagaacacg ccaaagcaca atcttcccaa
    24421 accgcaaaag tcatctacct cagcccccaa ggaaaaccgc tgacacacca aaaagcggca
    24481 gaactggcag aacttccgca tctgattctg ctgtgcggac gctatgaggg aatagacgaa
    24541 agactgctgc aaaccagcgt cgatgaagaa atcagcatcg gagacttcgt tgtttccggc
    24601 ggagagcttc ccgccatgat gctgatggat gcagtattga ggctcgtacc cggcgtattg
    24661 ggcgatatgc agtctgccga acaggattcg ttctcaagcg gcattttgga ctgcccccac
    24721 tacaccaaac ccttagaatt tcaaggcatg gctgttccgg aagtattgcg ttccggcaat
    24781 catggcttga tagcggaatg gcggttggaa caatcgctgc gccgcacctt ggagcgcaga
    24841 cccgacttat tggaaaagcg cagtttaatc ccaaaggaat cccgcctctt ggaaaccatc
    24901 cggcaagagc aacgggaaat ccaatcataa tttaggaaaa aaacaatgaa cctgattcaa
    24961 cagctcgagc aagaagaaat tgtccgcctg aacaaagaaa ttcccgaatt cgcaccgggc
    25021 gacaccgtag tcgtatccgt acgcgtcgtg gaaggtaccc gcagccgtct gcaagcctac
    25081 gaaggcgttg tcattgcacg ccgcaaccgt ggtctgaaca gcaacttcat cgtccgcaaa
    25141 atctccagcg gcgaaggtgt tgaacgtact ttccaactgt actctccgac cgtcgaaaaa
    25201 atcgaagtca aacgccgtgg tgacgtacgc cgtgccaaac tgtactacct gcgcggcctg
    25261 accggcaaag ctgcacgcat caaagaaaaa ctgcctgcac gcaaaggttg attcaaaccc
    25321 gttttcccca atgccgtctg aaccttctga cggcatttct tattgccgca acatccgcaa
    25381 cggaacagca ccaagaagca caccccttcc ggcacatccc gaatccccgt ccgaaccgaa
    25441 caaaatttac agaccaaacg ttatacagta tcatatcagc catataataa taaccgcttt
    25501 accgctttcc ctttcaaaac cgcaaatcat catgacacct tcccttttac tatcaggatt
    25561 gaccttccgc ctcgtccttg ccctgattgc cgtatccctt ttatggggcg tttacttttg
    25621 ggcagtatcc gcatgagcat cattgtcgaa aacctgaccg tcagctaccg ccgccgtcct
    25681 gccgtacacc acgttgacat tacttttgaa gaacatagta tgtgggcggt tttcggtccc
    25741 aacggcgcag ggaaatccac ctttctcaaa tccttgatgg gattgcagcc tatcgataca
    25801 ggcagcatcc ggctggatgg attgagccgc caagacatcg cctaccttcc ccagcagtcc
    25861 gatatcgacc gctcccaacc tatgactgtt ttcgacttgg cggcaatggg gctttggtat
    25921 gaaatcggct ttttcaaagg gataaatacc gctcaaaaac aacgcgttca cgaagccttg
    25981 gagcgtgtag gaatgcggca gtttgcacgc cgccaaatcg cccacctctc aaacggacaa
    26041 ttccaacgcg tcctttttgc ccgaatgctg gttcaaaatg caaaattcct gcttttggac
    26101 gaacctttca acgccgttga tgcacggacg acctacgaac ttctcgacgt attgcagaaa
    26161 tgccattgcg gcggacacgc tatcattgcc gtactgcacg attacgaaca agtccgtgcc
    26221 tactttccca gtaccctgct gctcgcccga gaaaaaattg cggcaggcgc aaccgagacc
    26281 attctgacag aaccctacct cgcccaagcc aacgccaaaa tgcagcaaca ggaaagcccc
    26341 gactggtgcg cctcataaat actgcccgaa accacgcccc acaccagaca ccatgaatct
    26401 ctacgacctg ctccttgccc cctttgcaga attcgacttt atgcgctacg ccctcgcatc
    26461 cgtcttctgc ctgtccctca gtgccgcacc cgtcggcgta ttcctcgtca tgcgccgtat
    26521 gagcctgata ggcgacgcat tgagccacgc cgtcctgccc ggtgccgccg tcggctacat
    26581 gtttgccggc ttgagcctgc ccgccatggg tttgggcggc gtagccgcag gcatgctgat
    26641 ggcactgctt gccggactcg tcagccgctt caccaccctg aaagaagatg ccaactttgc
    26701 tgccttttat ctcagcagcc tcgccatcgg cgtagtcctc gtcagcaaaa acgggagcag
    26761 cgtcgatttg ctccacctcc ttttcggctc tgtacttgcc gtcgatattc ctgccctgca
    26821 gctcatcgcc gccgtctcca gcctcacgct cattaccctt gccgtcatct accgcccgct
    26881 cgtactcgaa agcatcgacc ccctgtttct caaatccgtc ggcggcaaag gcgggctttg
    26941 gcacgtcctc tttctcgtcc tggtcgtcat gaacctcgta tccggctttc aagccctcgg
    27001 cacactcatg tccgtcggac tcatgatgct gccagccatt accgcccgcc tgtgggcgaa
    27061 gcacatgggc gcactcatcc tcctatccgt tctgacagcc ctgctgtgcg gcttgagcgg
    27121 actgctcatt tcctaccaca tcgaaattcc ctccggtccc gccatcatcc tctgttgcag
    27181 cgtcctttat ctcttttccg tcatactcgg caaagaaggc ggcattctga ccaaatggct
    27241 caaaaaccac cgccaccaca ccacctgacc ccgcaagccg cttgaacaaa caggcagctt
    27301 gttttaccat aacagatgtt acgttatatc acaggagaac agttatgaaa cacctcaaac
    27361 tcacccttat tgccgcattg ctaaccaccg ccgcaactgc cgcccccctg cccgtcgtaa
    27421 ccagcttcag cattttaggc gacgttgcca agcaaatcgg cggagagcgc gtatccatac
    27481 aaagtttggt cggagccaac caagatactc atgcctatca catgaccagt agcgacatta
    27541 aaaaaatccg cagtgcaaaa ctcgtcctgc tcaacggctt aggacttgaa gctgccgatg
    27601 tacaacgtgc cgtcaaacag agcaaagtat cctatgccga agcgaccaaa ggcatccaac
    27661 ccctcaaagc cgaagaagaa ggcggacacc atcatgacca cgaccatgac cacgaaggac
    27721 accaccatga ccacggcgaa tttgacccgc acgtctggaa cgaccccgtc cttatgtccg
    27781 cctatgccca aaacgtcgcc gaagccctga taaaggccga ccccgaaggc aaagtttatt
    27841 atcaacaacg cttgggcaac taccaaatgc agcttaaaaa actgcacagc gacgcacaag
    27901 ccgcatttaa cgccgtccct gccgccaaac gcaaagtcct gaccgggcac gatgcctttt
    27961 cctacatggg caaacgttac catattgaat tcatcgcccc gcaaggtgtg agcagcgaag
    28021 ccgagccttc agccaaacaa gtcgccgcca tcatccgaca aatcaaacgc gaaggcatca
    28081 aagccgtctt taccgaaaac atcaaggaca cccgtatggt tgaccgtatc gccaaagaaa
    28141 ccggtgtcaa cgtcagcggc aaactgtatt ccgacgcact cggcaacgcg cccgcagaca
    28201 cctacatcgg catgtaccgc cacaacatca aagccttgac caacgcgatg aagcaataaa
    28261 accgtttgat tggtttgaat ttgaaaaggc aaaaggccgt ctgaaaatta tagtggatta
    28321 acaaaaatca ggacaaggcg acgaagccgc agacagtaca gatagtacag taaggcgagg
    28381 caacgctgta ctggtttttg t
//
LOCUS       DAWFIE010000019        26706 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWFIE010000019 DAWFIE010000000
VERSION     DAWFIE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26706
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.019"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(173..601)
                     /locus_tag="WGQ71_000769"
     CDS             complement(173..601)
                     /locus_tag="WGQ71_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ0706818.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLGYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(666..2414)
                     /locus_tag="WGQ71_000770"
     CDS             complement(666..2414)
                     /locus_tag="WGQ71_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ0706819.1"
                     /translation="MIHYKITSSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHADSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKTLYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2741..4174
                     /gene="ccoN"
                     /locus_tag="WGQ71_000771"
     CDS             2741..4174
                     /gene="ccoN"
                     /locus_tag="WGQ71_000771"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ0706820.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4201..4812
                     /gene="ccoO"
                     /locus_tag="WGQ71_000772"
     CDS             4201..4812
                     /gene="ccoO"
                     /locus_tag="WGQ71_000772"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ0706821.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4817..4987
                     /locus_tag="WGQ71_000773"
     CDS             4817..4987
                     /locus_tag="WGQ71_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ0706822.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            5011..6108
                     /gene="ccoP"
                     /locus_tag="WGQ71_000774"
     CDS             5011..6108
                     /gene="ccoP"
                     /locus_tag="WGQ71_000774"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ0706823.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6221..6346
                     /locus_tag="WGQ71_000775"
     CDS             6221..6346
                     /locus_tag="WGQ71_000775"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706824.1"
                     /translation="MHKHAIEGFNTMPAKGGRGDLSADEVKAAVDYMVNQSGGKF"
     gene            6548..7957
                     /locus_tag="WGQ71_000776"
     CDS             6548..7957
                     /locus_tag="WGQ71_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0706825.1"
                     /translation="MDTQIKTEAGQQNNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRAEDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYHTMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            8058..11267
                     /gene="recC"
                     /locus_tag="WGQ71_000777"
     CDS             8058..11267
                     /gene="recC"
                     /locus_tag="WGQ71_000777"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ0706826.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPHIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWRQPATIGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLATAEAFAQKQDWEAALKKAQTAYHGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTLAAAEKKEEAGEA"
     gene            complement(11337..12905)
                     /gene="mtrF"
                     /locus_tag="WGQ71_000778"
     CDS             complement(11337..12905)
                     /gene="mtrF"
                     /locus_tag="WGQ71_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ0706827.1"
                     /translation="MNQTDTRRGGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIYVVSLLNADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAVI
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTLDPILAGITQQAAQIIHPDYAVGPEAN
                     WFFMAVSTFVIALIGYFVTEKIVEPQLGLYQSDLSQEEKDFRHSNEITPLEYKGLIWA
                     GAVFVALSALLAWSIVPADGILRHPETGLVAGSPFFKSIVVFIFLLFALPGIVYGQIT
                     RSLRGEREVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGAVFLKEVG
                     LGGSVLFVGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMGYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13237..13449
                     /locus_tag="WGQ71_000779"
     CDS             13237..13449
                     /locus_tag="WGQ71_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706828.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIARDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
     gene            complement(13728..14360)
                     /gene="mtrR"
                     /locus_tag="WGQ71_000780"
     CDS             complement(13728..14360)
                     /gene="mtrR"
                     /locus_tag="WGQ71_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ0706829.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDTEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLESHPCLRRK"
     gene            14764..16002
                     /gene="mtrC"
                     /locus_tag="WGQ71_000781"
     CDS             14764..16002
                     /gene="mtrC"
                     /locus_tag="WGQ71_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ0706830.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPAVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
     gene            16014..19217
                     /gene="mtrD"
                     /locus_tag="WGQ71_000782"
     CDS             16014..19217
                     /gene="mtrD"
                     /locus_tag="WGQ71_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ0706831.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            19272..20675
                     /gene="mtrE"
                     /locus_tag="WGQ71_000783"
     CDS             19272..20675
                     /gene="mtrE"
                     /locus_tag="WGQ71_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ0706832.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVDTHSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDAAKLRQQAQIVAYEAAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(20867..21046)
                     /locus_tag="WGQ71_000784"
     CDS             complement(20867..21046)
                     /locus_tag="WGQ71_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tonB dependent receptor family protein"
                     /protein_id="HEZ0706833.1"
                     /translation="MTQRPYATLYLTAHYKIGKSTRIGLDFENVFNKRYRPMPDIHVY
                     GTPRSLTATVKHIVD"
     gene            complement(21043..21342)
                     /locus_tag="WGQ71_000785"
     CDS             complement(21043..21342)
                     /locus_tag="WGQ71_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0706834.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPAGMAVSSLPPQPH"
     gene            21773..22552
                     /locus_tag="WGQ71_000786"
     CDS             21773..22552
                     /locus_tag="WGQ71_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ0706835.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            complement(22738..22995)
                     /locus_tag="WGQ71_000787"
     CDS             complement(22738..22995)
                     /locus_tag="WGQ71_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ0706836.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
     gene            complement(23039..23413)
                     /locus_tag="WGQ71_000788"
     CDS             complement(23039..23413)
                     /locus_tag="WGQ71_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706837.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            complement(23555..24889)
                     /gene="gdhA"
                     /locus_tag="WGQ71_000789"
     CDS             complement(23555..24889)
                     /gene="gdhA"
                     /locus_tag="WGQ71_000789"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ0706838.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            25312..26106
                     /locus_tag="WGQ71_000790"
     CDS             25312..26106
                     /locus_tag="WGQ71_000790"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ0706839.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFIHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            26115..26396
                     /locus_tag="WGQ71_000791"
     CDS             26115..26396
                     /locus_tag="WGQ71_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
                     /protein_id="HEZ0706840.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNGDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRRAADLVNKVLAEVARLEKIVQPLP"
     gene            26377..>26706
                     /locus_tag="WGQ71_000792"
     CDS             26377..>26706
                     /locus_tag="WGQ71_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0706841.1"
                     /translation="MFSLYREDSLISRLNRDGYLTSPSADFLELLSLCRDIHALTDGA
                     FDPTVQALWKLYADYFAARPDAETPPPQNSVDEALKRVGFGKVSFDDREIRFAEKGMG
                     LSLNGIAQ"
BASE COUNT         6450 a         6502 c         7173 g         6581 t
ORIGIN      
        1 tgcattttga aaatgagttt gagcataaaa ttttagtaac ctatgttatt gcaaaggtct
       61 cccactgtaa aaacggagaa ggacagagga cggcagtaag gtttgccgtc ctctttttca
      121 agttgcggtg gaatgccgct tgaaggtgtg gtttaaacgt ttcggcttgt aatcagacgc
      181 gggcgttgtc cagccgctct ctgaagtttt ccaaatttac gcctgccgct tgggcgcggc
      241 ggatgatttc gtcaaccggc ataccttctg ccgcccggcg gaagccccac aggagggagc
      301 agcgcgtacc ggtccggcaa tagccaagga cgggatattc ggcttgtccg atgagttggc
      361 ggaaggtttc gacatcgtgt ttttggatgt cgcgtgcggt aacgggttgg tgatggaatc
      421 cagtaacgcc tgcttgttcc agccactgtt tgatttgggc gaagtcgggt tgcgattctt
      481 cttcgcggtc ggggcggttg cagatgacgg ttttgatgcc cagttgcgcg atttgttccg
      541 catcggcttt ggtcagttgc ggagaaatat agaggtgttc gtcaagtttc agaatggcca
      601 ttttgtttcc tttactttgc ggttgttttt cagacggcat tttgtgtgca atgccgtctg
      661 aaagtttaac cgaacaacca gttgtccaac aggtttcggt ctgtgatatg taggatggca
      721 gtatccgctg ccgctgcctg aaccgtcaag acggtttgac gcaatatgcc ggcacggaag
      781 aagtggatat tgatttttgc attgacgtgg tatcgggccc attgtgcggt aaagtcggtg
      841 caggcataac cgtctaaagc aatgattttg tcttgcgggc acagtgccgc agattccgcg
      901 ctgccgccgt tgaagacatg ggtcaggacg atgtggtcgg tgttttgttt gaaacgtgcg
      961 ccaaaatcgc ctgccgacgg gacggggcag atgtgttctg cgtatccgcc gccgtgttgt
     1021 cggggaagcg gcaggaaggt cagtcccacg cctgcggttg ccaggcattc ggcaagcggc
     1081 aaatcttcgg tactgtataa cgttttttgg aaaaaatctt ccaaatccaa gccggtaatt
     1141 tcctgacagc ggatttgcca gtgtttttcc ggaatacccg agtgtgtgtc cctccactcc
     1201 cgatagagtt tgtccattaa cgtatcgaga gaatgtctgc cgttgcttcg gttgcgtatt
     1261 atcagatcaa ggcacaatgc ggcaagcgcg cctttctggt agtagctgac gatggcgttg
     1321 gggctgtttt catccggttt gtaaaatttg ttccacgcgg taaaactcga ttccgccaag
     1381 gtctgcctca aacggccgcg ggtttgttgt acgcgcgtaa tgccttgtgc cagcaggttt
     1441 aaataagatt cgggcgagat ggtgcggctg cgtgccaaaa acaaatcgtc gtaataggat
     1501 gtaatgcctt cgaatgccca tagttgttcg gtatagtttt ctttgtcgag gtcataaggg
     1561 acgaacgcgg caggtttgat ggatttgacg ttccacgcgt gaaaatattc gtgggagaaa
     1621 agtccgagca atgtggtgta ggtatcgtcg gcatcggtca taccgtacgg cggaaggctg
     1681 tggcggtcgg cgagcagggc ggtgctgtcg gcgtgttcca aaccgccgta aatatggtcg
     1741 ccgacgtgga gcaggaacaa atatttttga aacggggcag gggaggaaaa caccgccagt
     1801 tctgtttcgc agattttttt gatatccgaa accagcctgt tgcggtcgaa atcgggatat
     1861 atgccgctta aggcaattgt gtgcggaatg cctgccgcct caaaatctaa aaattcaatc
     1921 aagcccatct cgacaggtcg gtcaatcaat tcggcataag atgccgcctg aaagacaaac
     1981 ctccctgttt ccggcagcgt tgtggcaata cgccattcgg acggaatacc cgtcaattcc
     2041 aagcggtgcg gcagcgtttc cgttccttcg actttcaaaa acaggcacga tccgtcaaaa
     2101 aaaccgcgtt ctgtcgtcag gaaagaacct cgaaccgaca aatcgaatgc atatacggtg
     2161 tagcggattt gccactcgcc gcgtacggcg gcagcgtgcc agcggttttt tgcaatttgt
     2221 tcgagcggca tggacgtgcc gttacaggat gcatggatag aagtgatgtg gcgggaaaaa
     2281 tcccgaatca gatagctgcc cggaacccag tttggcaggc ttatttctat aggaagatca
     2341 tcatcttgtg tgaatgtcag caggatgtgc cactcgtggt cgagagggga cgaggttatt
     2401 ttataatgaa tcataactat caaatatatc gttaaacggg atggattaaa tacctttaaa
     2461 atgcagctta acctacatca ataaagtgcc acaaaaaggg aaaattggaa acaaaagcaa
     2521 tggcggtcgg gttgtaattt attataacaa tatgatttat atgtaataaa aaacagatta
     2581 agcaaagagc tcgccggcgg cgcgaatgta attccgcttg gcgtaataag gcagatatgt
     2641 taaatttaga aaaaattacg cttgcccgga ttgcacctta aacaggctgc ggaagcggca
     2701 ggataaaaat ctttattccc ataaattcat ggagactctc atggacacac aaacttacaa
     2761 ctacaaagtg gtgcgccagt tcgccatcat gactgtagtt tgggggattg tgggtatgtt
     2821 ggtcggcgtt atcgtcgccg cccagctttt tgctcctgcc ctcgacttgt ctaatatcgg
     2881 accttggttc cacttcggcc gcctgcgtcc gctgcacacc aatgcggtta ttttcgcatt
     2941 tggcggttgc ggcctgatcg gcacatcata ctacgttgtc caacgtactt gtaatacccg
     3001 tcttttcggc ggttggctgc cggcatttac cttttggggc tggcaggcgg taatcgttgc
     3061 cgccgtcgtc agcttcccta tgggttggac ccaaggtaag gaatatgccg aactggaatg
     3121 gccgatcgat attctgatta ctttggtttg ggtggcttac gccatcgtat tcttcggtac
     3181 gattgccaaa cgtaagatta aacatattta cgttgccaac tggttctacg gcggctttat
     3241 tttggcggtc gcacttttac acatcgtcaa caatatcagc atcccggcag ggttgatgaa
     3301 gtcatacccc gtctattccg gtgcgattga tgctatggtt caatggtggt acgggcataa
     3361 cgccgtgggc ttcttcctga ctgccggctt cttgggtatg atgtactatt tcgtacccaa
     3421 acaagcagcc cgccccgttt actcctaccg cctgtccgtc gttcacttct gggcgttgat
     3481 tttcacctat atgtgggcgg gtccgcacca tcttcactac actgcgctgc ctgactggac
     3541 gcaatctttg ggtatggttc tgtctttgat tctgttcgca ccctcttggg gcggtatgat
     3601 taacggcatc atgaccttgt ccggcgcgtg ggacaaactg cgtacagacc cgattcttaa
     3661 attcctgatt gtatccttgt ccttctacgg tatgtctacc tttgaaggcc cgatgatgtc
     3721 gattaaaacg gtcaatgcat tgagccacta tacggactgg accgtcgcgc acgttcatgc
     3781 gggtgcgttg ggctgggtag gctttgtaac catcggttcc gtctattaca tgattccccg
     3841 tctgttcggc aaagaacaga tgcacagcac caagctggta gaagcacatt tttggattgc
     3901 gaccatcggc gtggttctgt atatcgctgc catgtggatt gccggtgtga tgcagggttt
     3961 gatgtggagt tctttgaacg atgacggtac gctgacctac tcgtttgtcg aatccgtaaa
     4021 acgcaccatg ccttactacg tgatccgttt tgcaggtggt ttattgtatc tgagtggtat
     4081 gtgcattatg gcgtacaacg tgtaccgcac agccatcggt ggtaaagcag tcgatgccga
     4141 aatccctgcg gtttcccaaa cacagcacca ctaaaatact aagaaagata ggctaccaaa
     4201 atgaaattac aacaattggc tgaagaaaaa atcggcgttc tgattgtgtt cacgctgctt
     4261 gtagtcagtg tcggtctgtt gattgaagtt gtgcccttgg cctttaccaa ggcggcaaca
     4321 cagccggcgc cgggcgtgaa gccttacaat gccctgcagg ttgccggacg cgatatttac
     4381 atccgtgagg gctgttacaa ctgccactcg caaatgattc gtccgttccg tgcggaaacc
     4441 gagcgttacg gtcattactc tgttgccgga gagtcggttt acgaccatcc gttccaatgg
     4501 ggttccaaac gtaccggtcc tgatttggca cgtgtgggcg gtcgctattc cgacgaatgg
     4561 caccgtatcc acctgctgaa tccccgtgat gtcgtgcctg agtccaatat gccggcattc
     4621 ccgtggcttg cacgcaataa agttgatgtc gatgcaaccg ttgccaacat gaaggctttg
     4681 cgtaaagtag gtactcctta cagtgatgag gaaattgcga aagcacctga ggctttggca
     4741 aacaaatccg agctggatgc tgtagtcgcc tatctgcaag gattgggtct ggctttgaaa
     4801 aacgtaaggt aacatcatgg atattaacgg tattcgtgct ctcttcacgg tatggatctt
     4861 tatctgtttc ctgttggtac tctatatcgt cttcaacagg cggaataaga aaaactacga
     4921 taatgccgca aacagcattt ttgatgaaaa ccaagatgcg caagataaga aaagcgaaaa
     4981 ccgttaatat tgtgataacg gagcaaaaca atgaacacaa catcccaatt taccagtaat
     5041 ttctggaata tatatattgc agttattgtc ttactgagct ttatcgcttt ggcttggctg
     5101 ctgctgtctc aaaatgttgt caaacgtccg aagaagggcg aagaagtaca aactacgggt
     5161 catgagtggg acggcattgc cgaatacgac aatccgctgc cccgctggtg gttttggctg
     5221 tgtgttttga cgtggctgtt cggtatcggt tatttggtta tgtatccggg tgtcggcgac
     5281 tacaaaggtc tgctgaaatg gaccagccat aaccaatatg aaaaagaggt caaaaaagcc
     5341 gatgagcaat acggcaaact gtatgccaag tttgcggata tgccgattga aaaagtggca
     5401 aaagaccctc aagccaagca aatcgcccaa aacctgttta acacttattg tatccagtgc
     5461 cacggctctg atgctaaagg ctctaaaggt tttccgaatc tgaccgatag cgattggttg
     5521 tggggcggtg atcccgataa aatccacgag accatcgaaa aaggccgtgt tgcgactatg
     5581 cctgcctggg gtcctgcttt gggcgaagaa ggcgtgaaag acgttgccca ttatgtgatg
     5641 tccctttcaa aacccgaagg tcagtatgat gaggaacgtg ccgcgcgcgg acaagccttg
     5701 ttcagcggtc cgcctgccaa ctgtttcact tgccacggcg ataagggaca aggtatccaa
     5761 ggtttgggtc cgaacctgac tgatgacgtg tggttgtggg gcggtacgca aaaatccatt
     5821 atcgaaacca ttaccaacgg tcgcagcagc caaatgcccg cttggggaca tttcttggat
     5881 aaagacaaac tgcatattat gactgcttat gtatggggtc tttccgataa agacggtaaa
     5941 gctccggtga aaaaagccga gcctgcaccg actcccgcac cggcggcaga acccgcagcc
     6001 tctgctcctg cagaagcagc acaagccgcg tccgaagcca aacctgccgc agcagaacct
     6061 aaagccgagg aaaaagccgc acctgctgcc aaagcggacg gcaaataggt ttatgaaacc
     6121 gtttgtgccg cctgccatgg caatgcgatt ccgggtattc cccatgtagg catcaaagcc
     6181 gattgggccg accgcatcaa aaaaggcaag gacacgttcg ttgcacaaac acgcgatcga
     6241 aggtttcaat acgatgcccg caaaaggcgg tcgcggcgat ttgagcgctg atgaagtgaa
     6301 agctgcggtt gactatatgg tcaaccagtc aggcggcaaa ttctaacttg actgagtttc
     6361 cgatattatc ggttatgaaa atccagattc agtattgaat ctggattttt tgttttaacg
     6421 ggcatccggt tttaaacgca gactgcctat ttgttgtaat gaattttgac tttagtcata
     6481 aagaattagt ctttattgtt tataatcgcg catcatcacg atagccctat tttcaaaagg
     6541 acgattaatg gatacacaaa tcaaaactga agccggtcag cagaataacc ggcgttatct
     6601 gaccgtttgg cggtggcatt tttatgccgg tctgctggtt atgccttttc ttaccctgct
     6661 tgccgttacg ggtttgggta tgctgctgtt tgccaatatt accggtaagg agggcgagcg
     6721 gattcatgtt gtgccgcagg caacggtaca acctctgtct gttcaggcgg aagcggcacg
     6781 cagtgccgtt aatccggaaa cttcgtccgt cgttcagtat attgcgccgc gcgccgagga
     6841 catggttgcc gtgttccgtg tcaacaatga gggtaaggca acgatggtcg cggtcgatcc
     6901 ttatacggca aaagtagtca gcactatgcc gcgcaatcag ggttggtatc acacgatgga
     6961 tgaaatccac agcgatatga tgctcggttc gacaggcgat tatcttttgg aaacggctgc
     7021 atcgctgacg attatcatga taatcagcgg tttgtacctt tggtgggtga aacggcgcgg
     7081 catcaaggcg atgctgctgc cgtcaaaagg cagggcgcgt tcttggtggc ggaatctgca
     7141 cggcacgttt ggaacttggg tgtcgttgat tttgctgttg ttctgcctgt cgggtattgc
     7201 ttgggcgggt atttggggcg gcaagttcgt acaggcttgg agtcagttcc ctgccggtaa
     7261 atggggtgtc gaaccgaacc ccgtttcagt cgtgccgacc cacggcgagg tattgaatga
     7321 cggcaaggtt aaggaagtgc cgtgggtttt ggagcttacg cctatgcctg tttcagggac
     7381 gactgtgggc aaagacggca ttaaccctga cgagccgatg acattggaaa ccgtcgaccg
     7441 ctttgcgcgg gaaatcggtt tcaaagggcg ttatcagttg aatttgccca aaggcgagga
     7501 cggcgtatgg actttgtcgc aggattctat gagttacgac atgatcagcc cgtttgccga
     7561 ccgcacggta catatcgacc agtacagcgg caaaatcctt gccgacatcc gttttgacga
     7621 ttacaacccg ttcggcaaat ttatggcggc aagcattgcg ctgcatatgg ggactctggg
     7681 ctggtggagc gtgttggcga acgtcttgtt ctgccttgcc gtcattttta tcggtatcag
     7741 cggctgcgtg atgtggtgga aacgccgtcc gaccggagcg gtgggcatcg ttccgccggc
     7801 gcagaaagtc aagctgccgg tttggtggat gatggcattg ccgctattgg caatcgcact
     7861 gctcttcccg accgcactgc ttgccattgc cgtgatttgg ctgttggata cgctgctgtt
     7921 gtcgcggatt cctgttttga ggagatggtt taaatgacca aatgccgtct gaaaggttca
     7981 gacggcattt tgtttgaagg cggacggggg aaaggctata taatcccgaa tacttttccg
     8041 caacttttgt cgaaatcatg ttttatctgt atcaatccaa ccgtcttgaa acgctggcgg
     8101 cattgtttgc ccgcattcaa aaagtcaaac cgctgaaatc ggctttacag cccgaacaga
     8161 ttattgtgca gagtcagggg atgcgccgct atctcaatac ctgcctcgcc cgcgatttgg
     8221 gcgtggcggc gaatttgtcg ttcagcctgc ccgccggcct gacgtggaag ctgatgaaaa
     8281 aactgattcc cggtattccg gaactcagcc cgtttgcacc cgaagtcatg cgctggcggc
     8341 tgctggattt gttccgcagt gaagcattcc ggaatacggc agaatttgaa gatgtgagga
     8401 atgtgctgca agactatctg ggcagcggcg aatcggcaga ttaccagctt gcgggacagc
     8461 ttgcggacat attcgaccaa tacctcgtgt accgtcctca gtggatagac gcttggcagc
     8521 agggcaggcg gctcggtttg ggcgacgacg aaatctggca gtccaaactg tggcgttacc
     8581 tcgacgatgg caggcagagc gcgccgcacc gtgtcgcgtt gtgggaaaag ctgcttgccg
     8641 cgttggacaa ggataagctg cccgagcgtt atttcgtgtt cggcatttcc acgatggcgc
     8701 cgatgtatct tcagcttttg cacaagctgt ccgaacattg cgatgtgttc gtgttcgcac
     8761 tcaatccgag cgggatgtat tggggcaacg tcatcgaagc ggcgcaaatc ctcaaaggcg
     8821 gcggcgatcc cgatttaact caggcagggc atccgctgct cgcctcattg ggcaagcagg
     8881 ggcgcgactt tttcgacttt ttgaacgaaa tggaaataga aggagaaacg ccggtatttg
     8941 aagaaggcgg gcgcgatacg cttttgcacg ccctgcaaac cgatattcaa aacctgaaaa
     9001 tgccgtctga aaatgtggga agcgtcaaca cgggcgacgg ctcgatacgc atcgtctccg
     9061 cacacagccc cttgcgcgaa ttgcagatac tcaaagacaa gctgttgaaa attctgcatg
     9121 aacatcccga ttggcagccg catgatatcg ccgtattaac cccgcacatc gaatcctata
     9181 cgccttttat cgaagccgtg ttcggacagg cgcagcccgg tgcgcaggca ttgccgtatt
     9241 ccgtttcaga cgtgaaaatc agccgccgac aaccgctgtt tcatgctttg tcatgcctgt
     9301 tcgacttgtt ggaaagccga tttgaagtcg ataaagtgct tgtgctttta gaaaccgcac
     9361 ccgtgttgcg ccgtttcgga ctgactgagg acgatttgcc gcttttgcac gatatggttg
     9421 ccgatttgaa cgttcactgg ggtttggacg gagaaatgcg cggcggcacg gatcagctgt
     9481 ttacctggaa gcaggcggta gaacgcatga tattgggctg gatgctgccc gaaggcggca
     9541 acccgatgtg gcaggatgtc agcgcgtggt atgccgacgt gaaccaaacc gccctgttcg
     9601 gacgttttgc cgccctcctc gaaaccctgt cggatattgt acggatatgg cggcagcccg
     9661 caacgatcgg cgaatgggtg gcgcgttgcc gggatttgct tgaaacattg ttccaagctg
     9721 aagccgatga ccaaaagtca gtccaaaacc ttgaaaacga atgggtcaaa tggcaggcgg
     9781 aaacagaatt ggcgcaattt tccggacagt tgccgcaaca aaccgtcatc cgccatatcc
     9841 gacgtttcct cgacagcgaa agcgaggcag gctttttacg cggcggcatc accttttgca
     9901 gtatggtgcc gatgcggagc ctgccgttca aagtcatctg cctgttgggt ttgaacgacg
     9961 gagattttcc ccgcaatacc aaagccgccg tattcgacct gattgccaaa catcccgcca
    10021 aaggcgaccg cgcccgccgc gatgacgacc gctacctctt cctcgaagcc ctcatcagcg
    10081 cgcgcgaaat cctctacctg tcctacatcg ggcgcgacat ccgcaaagac gaagagcttg
    10141 ctccgtcttc gctgttgggc gaactcatcg acacagttgc cgctatggcg ggcatcggta
    10201 gccgccaact tgcacaaaac tggatagaac agcatccgct gcaagccttc tcgcgccgat
    10261 attttcaaga aggcggacgt tcagacggca tattcggcac gcgtaccgac tacgccactg
    10321 cgctcggaca aacgcccgaa ccgccgcaac cctttttcga ccaacccgta gaaaacgccg
    10381 aacctgttgc cgaaatcgga caggacgaat ttatccgttt ctggcgcaac cccgtcaaag
    10441 tatggcttca gcagcagctt gcgtggagcg aaccccatat cggcgaagcc tgggagcctg
    10501 ccgaaccctt cgagccgcaa cacgccgatc aaatcgccga aacctacatc gaagcacggc
    10561 gcgaaggacg ggattttgcc caaaccgccg cccgcatcgg ggcggaaagc ctcctgccgt
    10621 cgggagagtt gggcagactt tggcagcagg acttccaaac cgctgccaaa caaatcgaca
    10681 cggcggtttt aaacagtccc aaacttcctc cgctgcccta cgccataccg tcggacgggc
    10741 aaatcctgaa aggcagcttg ggcaatctgt accgctgcgg acaagtgttt tacgcctacg
    10801 gcaaacccaa cgcgccgcaa cgtatcgctt ttttgttgga acacctgata ttttgcgccg
    10861 ttatgccgtc tgaagccgaa acgcggcaaa cctttatcgt ccaatccgga gaaaccgaaa
    10921 tattggcgga aatcgcgcaa gacagggcat tgcagctatt gtcggaatgg atggcgtttt
    10981 tcaatatcgg gcaaaaccgc ccgctgccgt tttttgccaa aacctcgctt gccaccgccg
    11041 aagcgtttgc ccaaaaacaa gattgggaag ccgccctgaa aaaagcccaa accgcctacc
    11101 acggcagcaa agtcagcaaa gggcagaaag actataccga agtcgccctc gtgttcggca
    11161 acgcaagcca aaacccgctc gaacagcccc tgtttgaaaa cctcgtccgt ctgcttgccg
    11221 acacacttgc cgcagcggaa aaaaaggaag aggccggaga agcctgacgg gatagaaagg
    11281 caaatgccgt ctgaaaacag caaaaaggtt ttcagacggc attttgttta tcgtgtttaa
    11341 ggtgcgggat agaatgtggg cgcgccggga ccgacgggca ggcccaaaac aaatacccaa
    11401 atgcagaata aggcaatcca agcaatcaaa aagaaggcgg aatacggcaa catcatagaa
    11461 atcagcgtac ccacgcccgc atcttttttg tatttgatca ccgtcgccat aatcagcccg
    11521 aaataaccca tcatcggcgt aataatattg gtaacggaat caccgatgcg gtaagcggct
    11581 tgaatgactt cgggcgcgta gccggccaac atcagcatag ggacaaaaat cggtgcggtt
    11641 accgcccatt gcgcggaagc ggagcctatc atcagattga taaaggcaca aattaaaata
    11701 aaaccgacaa acaacacgct gccgcccagg ccgacttctt ttaagaacac cgccccttta
    11761 acggcaatat attgcccgat attcgtccaa ttaaaaaatg cgacaaactg tgcggcaaaa
    11821 aagatgatga ccaaataaag tcccaaagta ctcatcgatt cggccatcgc attaacgact
    11881 tcccgttcgc cgcgcaaact tcgggttatc tgtccataaa caatgcccgg tagcgcaaac
    11941 aacaagaaaa taaaaacaac aatcgatttg aaaaacggcg aacctgcaac caatcctgtt
    12001 tcaggatgac gcaaaatgcc gtcggcaggg acgatgctcc aagccaataa ggcggataaa
    12061 gcaacaaaca ccgcgcctgc ccaaatcaat cctttatatt ccaaaggcgt gatttcattg
    12121 gaatgccgaa agtctttttc ttcttgtgac aaatctgact gataaaggcc caattgcggt
    12181 tcgacgattt tttcagtaac aaaataaccg atcaaagcaa tcacaaacgt actgaccgcc
    12241 ataaaaaacc aattggcttc agggcctacg gcgtagtcgg gatggatgat ttgcgccgcc
    12301 tgttgggtga tgcctgccaa gatcggatcg agcgtgccta agaacaaatt ggccgaataa
    12361 ccgcccgaaa caccggcgaa agccgcagcc aaaccggcaa gcggatggcg gccgagcgaa
    12421 tgaaagatga cggcggacaa agggattagg acgacataac ccaattcaga agccgtatta
    12481 gacaaaatcc ctgtaaaaac aaccataaaa gtggtaagtt tgcgtggcga ttttgtgagc
    12541 aataagcgca tcaatgcaga aatcaagccc gatttttccg caatccccac gcccaataaa
    12601 gaaaccaaca ccgttcccaa cggcgcgaaa ccggtgaaat ttttaacggt atgcgtcagg
    12661 attttgatca aaccgtcggc attgagcagg ctgacaacgt aaatcaaacc gtcatcggca
    12721 cgtcctttcg caccaacagg gcgcggatcg gggacggata gtccgaaata cgcaccgacg
    12781 gcagaggcaa tcagcaataa cacaatgaaa ataataaaaa gcgtaaccgg atgcggcaac
    12841 atattgccca gccattcgac tgtgcgtaaa aatcgcccgc cccgccgcgt atcggtttga
    12901 ttcatctgtt tctccttaaa gaaatatatc gtgtgcatta aatttttgaa tgtaacatga
    12961 agtgttttgt aagcaaacta aaataatcgc atatgtatat aaccgcaata gattaaaaca
    13021 gaaaatgccc tcatcgttat tctcataaag gcgggaatcc gaaagctcga cgttaaagcc
    13081 ttatcaggaa tgactggaac tcaaaaaacg gtttcccgct ttcgcggaaa tggcgggatt
    13141 ttcggttttt atttgcaaaa atgccggaga agaatgcagt ttacattttg aatggtattg
    13201 ttattaattg taaataaata ttggaaggta aatgatatga aaaatgaaat tcaaaaaatt
    13261 atggacaaat atgatccctg gcatgaagat gattttgagt cgtatgaaga cattgccaga
    13321 gatgtatcgc tgatgacaga taaaacgttc attgaacatt atttgttaga agtttattca
    13381 gaagaaaacg gacattttga ccaagagaat gtccatgcaa tgatagaaga aattaaaaat
    13441 gcaatttagt gaatatgaga cctttgcaaa aatagtctgt taacgaaatt tgacgcataa
    13501 aaatgcgcca aaaaattttc aattgcctaa aaccttccta atattgagca aaaagtagga
    13561 aaaatcagaa aagttttgca ttttgaaaat gagattgagc ataaaatttt agtaacctat
    13621 gttattgcaa aggtctcaat atgtattttt agaagaaggc tttgtgtcat tttgatgccg
    13681 tctgaaaggg tttgttcgtt tcagacggca ttgttaccaa ggcttgatta tttccggcgc
    13741 aggcagggat ggctttccaa gttgtccatc attatcccga tgatgcgcgg ggcggttttg
    13801 cccaaatcga aacgttcgca ggaagagaac caccgccaaa tcagcccgtc caaggttgat
    13861 ttgatgaaga taactgccgt ttccttgtcc aaatcgtcag ccaaatcctg attttccacc
    13921 gcttcggtca aaacggcggt aattttctcg cgccagattg cctgatgctt gcgggcaatg
    13981 gcgataacgg cggcgttttg ctccgtgtgt tcgcatttta aaaacaggat gttgtggaat
    14041 ttgtagtaga tgtcgttgct ttgcagccgc tcgaaaaagt gcagcagcgt gtggcggaat
    14101 accgcccaag accctccttc ggcatcttcg gtatcttgcg cgatgcagtt ttcgatgtcg
    14161 tcgcagatac gttggaacag cgcgtcgaac aagtcttcct tatttttgaa atgccaatag
    14221 agcgcgccgc gcgttacgcc ggcggcttgg gcgatttcgt tgagcgaggt gcgcgcaatc
    14281 cctttgcggt aaaaggtttc caaggcggca agcatcaggt gttctttggt tttcaaggct
    14341 tcggttttgg tttttctcat aatggcgttt tcgtttcggg tcggtttgat gagggcggat
    14401 tataaaaaag actttgtaac catgcaatcg tgtatgtata atgaaaccca tgaaattgag
    14461 actacatctc aactttgaaa acaggcataa cctgctttgc aacccgttcg aacattcggg
    14521 tatcaaagca ggcggttttt atatcctgaa atatagtgga ttaacaaaaa tcaggacaag
    14581 gcgacgaagc cgcagacagt acaaatagta cggaatcgat tcacttggtg cttgagcacc
    14641 ttagagaatc gttctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc
    14701 actataagaa tgcctgttga ttggcaaaac cctttatatc aaaccagaca gataggataa
    14761 ataatggctt tttatgcttt taaggcgatg cgtgcggccg cgttggctgc cgccgttgca
    14821 ttggtactgt cgtcttgcgg taaaggcgga gacgcggcgc agggcgggca gcctgctggt
    14881 cgggaagccc ctgcgcccgt cgtcggtgtc gtaaccgtcc atccgcaaac cgtcgcattg
    14941 accgtcgagt tgccggggcg tttggaatcg ctgcgtaccg ccgatgtccg cgcccaagtc
    15001 ggcggcatca tccaaaaacg cctgttccaa gaaggcagtt atgtccgtgc cggacagccg
    15061 ctgtatcaga tcgacagttc cacttatgaa gcaggtctgg aaagcgcgcg cgcgcaactg
    15121 gcaacggctc aggcaacgct tgccaaagcg gatgcggatt tggcgcgata caagcctttg
    15181 gttgccgccg aagccgtcag ccggcaggaa tacgatgctg cggtaacggc gaaacgttct
    15241 gccgaggcag gtgtcaaagc agcacaggcg gcaatcaaat ctgccggcat taatctgaac
    15301 cgttcgcgca ttaccgcgcc gatttccggc tttatcggtc agtccaaagt ttccgaaggt
    15361 acgctgttga atgcgggcga tacgaccgtg ctggcaacca tccgccaaac caatccgatg
    15421 tatgtgaacg ttacccagtc tgcatccgaa gtgatgaaac tgcgccgtca gatagccgaa
    15481 ggcaaactgc tggcggcgga tggtgtgatt gcggtcggca tcaaatttga cgacggcaca
    15541 gtttaccctg aaaaaggccg cctgctgttt gccgatccgg ccgtcaacga atcgaccggt
    15601 cagattaccc tgcgcgccgc cgtaccgaac gatcagaata tcctgatgcc cggcctgtat
    15661 gtgcgcgtgc tgatggacca agtggcggtg gataatgcat tcatcgttcc acagcaggca
    15721 gtaacgcgcg gtgcgaaaga taccgtgatg attgtgaatg cccaaggcgg tatggaaccc
    15781 cgcgaggtaa cggtcgcgca acagcagggt acgaattgga ttgttacgtc gggtctgaag
    15841 gacggggaca aggtggttgt ggaaggcatc agtatcgccg gtataacggg tgcgaaaaag
    15901 gtaacgccca aagaatgggc gccgtctgaa aaccaagccg ccgcgcctca atccggcgtt
    15961 cagacggcat ctgaagccaa aactgcttct gaagcggaat aaggaaggca tcgatggcta
    16021 aattttttat cgaccgccct atttttgcgt gggttatttc gattttcatt attgcggcgg
    16081 gtattttcgg catcaaaagc ctgcctgttt cgcaatatcc gtctgtcgcc gccccgacca
    16141 ttactttgca cgccacttat ccgggtgcgt ccgcgcaggt aatggaagac agcgtgcttt
    16201 ccgtgatcga gcggaatatg aacggcgtgg aaggtttgga ttatatgtcc acttccgccg
    16261 attcgagcgg cagcggcagc gtgagcctga cctttacgcc cgataccgac gagaatctgg
    16321 cgcaggtgga agtgcagaac aagctttccg aagtattgag cacgctgccg gcaaccgtcc
    16381 agcaatacgg cgtaaccgta tccaaggcgc gttccaattt cctgatgatt gtgatgcttt
    16441 cgtcggatgt gcagtcaacc gaagagatga acgactacgc gcagcgtaat atcgttcccg
    16501 agttgcagcg tatcgaaggc gtggggcagg tacgcctgtt cggcgcgcaa cgcgcgatgc
    16561 ggatttgggt tgatcctaag aaactgcaaa actacaattt gtcgtttgcc gatatcggca
    16621 gtgcgctgtc cgcccagaac gtccagattt cagcgggttc tatcggttcg cttcccgccg
    16681 ttcgcggaca gacggttacg gctaccgtaa cggcgcaagg gcagttgggt acggcagaag
    16741 aattcggcaa cgtcatcctc cgcgccaata ccgacggttc taatgtttac ctgaaggatg
    16801 tggcaagggt cggactgggt atggaagact attcttcctc aacccgtctg aacggtgtaa
    16861 ataccaccgg tatggcggtg atgctgtcca acagcggcaa tgcgatggcg acggcaaagg
    16921 cggttaaaga acgcatggcg acgttggaaa aatactttcc tcagggtatg agctggaaaa
    16981 ccccttacga tacttccaaa ttcgtcgaaa tttcgattga aaaagtgatt cacactttaa
    17041 tcgaagcgat ggtgctggtg tttgtcgtaa tgtatctctt cctgcaaaac atccgttata
    17101 cgctgattcc gaccattgtc gtaccgattt cgctgttggg cggtttcgct ttcatctctt
    17161 atatgggcat gtcgattaac gtactgacca tgtttgcgat ggttttggtc atcggcatcg
    17221 tggtcgatga cgcgattgtg gtggttgaaa acgtcgagcg cattatggcg ggtgaaggct
    17281 tgccgcccaa agaagcgacc aaaaaagcga tgggtcagat ttcgggcgcg gtcatcggta
    17341 ttaccgccgt tctgatttcc gtgttcgtac cgttggcgat gttcagcggg gcgacgggca
    17401 atatttacaa acagtttgcc ctgacgatgg cgtcatcaat cgcattctcc gccttccttg
    17461 cgctgaccct tacccctgct ttgtgtgcca caatgttgaa gacaatcccg aaagggcatc
    17521 acgaagagaa aaaaggtttc ttcggctggt ttaacaagaa attcaacagt tggacgcacg
    17581 gttacgaagg ccgggttgcc aaagtgctgc gtaagacttt ccgcatgatg gttgtctata
    17641 tcggcttggc ggttgtgggc gtgttcctgt ttatgcgtct gccgacttca ttcctgccga
    17701 ccgaagacca aggcttcgtc atggtcagcg tgcaactgcc tgcaggagcg acccaagagc
    17761 gcaccaatgc gactttggcg caagttaccc aactggcgaa aagcattcct gaaatagaaa
    17821 acatcattac cgtttccggc ttcagctttt cgggcagcgg tcagaatatg gcgatgggtt
    17881 ttgccatatt gaaagattgg aacgagcgta ccgcgcccgg cagcgatgcc gttgcgattg
    17941 ccggcaagct gacgggtatg atgatgggga cgcttaaaga cggttttggc atcgccgtcg
    18001 tcccgcctcc gattctggag ttgggcaatg gttcgggcct gagcatcaac ctgcaagacc
    18061 gcaataatac cggccatacc gcattgctgg cgaagcgcaa cgagttgatt cagaaaatgc
    18121 gtgccagcgg tttgtttgac cccagcaccg tccgtgccgg cggtttggaa gactcgccgc
    18181 agttgaaaat cgacatcaac cgtgccgccg cggcggcgca aggcatttcg tttgccgaca
    18241 tccgcaccgc attggcaagc gcgctgagtt cgtcttatgt cagcgacttc ccgaaccaag
    18301 gccgtctgca acgcgtgatg gtgcaggccg acgaggatgc ccgtatgcag cctgccgata
    18361 ttttgaacct gaccgtgccg aacaaatccg gcgtcgccgt accgctttcc accattgcta
    18421 ctgtttcttg ggaaaacggt acggaacaga gcgtacgctt caacggttat ccttcgatga
    18481 agctgtccgc ttcgcctgca accggcgttt ccaccgggca ggctatggcg gcggttcaaa
    18541 aaatggttga cgaattgggc ggcggttaca gcttcgaatg gggcggacag tcgcgcgaag
    18601 aggcaaaagg cggctcgcaa actctgattt tgtacggttt ggcggttgca gccgtatttt
    18661 tggtacttgc cgcgctttat gaaagctggt cgattccgct ggcggtcatc cttgtgattc
    18721 cgttgggttt gatcggtgcg gctgcgggcg taaccgggcg caacctgttt gaaggactgt
    18781 tgggcagcgt tccctcgttc gccaacgaca tctactttca agtcggtttc gttaccgtga
    18841 tgggtttgag tgcgaaaaat gcgattttga ttatcgaatt tgccaaagac cttcaagcgc
    18901 aagggaaaag cgcggttgaa gccgctttgg aagcagcccg cctgcgtttc cgtccgatta
    18961 tcatgacctc gttcgccttt attttgggcg tggttcccct gtatattgcc ggcggtgcaa
    19021 gttctgccag ccagcgcgcc atcggtacaa ccgtattctg ggggatgctc atcggcacgc
    19081 tcttgtccgt gttccttgtt ccgcttttct atgtggtggt gcgcaaattc ttcaaagaaa
    19141 ccgcgcacga acacgaaatg gcagtaaaac acgctgccga agcgggcatc accggttcgg
    19201 acgacagcca acattaagca accatgccgt ctgaacgccc acgggttttc agacggcatc
    19261 aggacttttt tatggatact acattgaaaa ccaccttgac ttctgttgca gcagccttcg
    19321 cattatccgc ctgcaccatg attccccaat acgagcagcc caaagtcgaa gttgccgaaa
    19381 cgttcaaaaa cgataccgcc gacagcggca tccgtgcggt cgatttaggt tggcatgact
    19441 attttgccga cccgcgcctg caaaagctga tcgacatcgc actcgagcgc aataccagtt
    19501 tgcgtaccgc cgtattgaac agcgaaatct accgcaaaca atacatgatt gagcgcaaca
    19561 acctcctgcc cacgcttgcc gccaatgcga acggctcgcg ccaaggcagc ttgagcggcg
    19621 gcaatgtaag cagcagctac aaagtcggac tgggtgcggc atcttacgaa ctcgatctgt
    19681 tcgggcgtgt acgcagcagc agcgaggcgg cactgcaagg ctatttcgct agcaccgcca
    19741 accgcgatgc ggcacatttg agcctgattg ccaccgttgc caaagcctat ttcaacgaac
    19801 gttacgccga agaagcgatg tctttggcgc aacgtgtttt gaaaacgcgc gaggaaacct
    19861 acaagctgtc cgaattacgt tacaaggcag gcgtgatttc cgccgtcgcc ctacgtcagc
    19921 aggaagccct aatcgaatct gccaaagccg attatgccca tgccgcgcgc agccgcgaac
    19981 aggcgcgcaa tgccttggca accctgatta accaaccgat acccgacgac ctgcccgccg
    20041 gtttgccgtt ggacaagcag ttttttgttg agaagctgcc ggccggtttg agttccgaag
    20101 tattgctcga ccgtcccgat atccgtgctg ccgaacacgc gctcaaacag gcaaacgcca
    20161 atatcggtgc ggcacgcgcc gcctttttcc catccatccg cctgaccgga agcgtcgata
    20221 cgcattctgc cgaattgggc gggctgttca aaagcggcac gggcgtttgg tcgtttgcgc
    20281 cgtctattac cctgccgatt tttacctggg gcacgaacaa agccaacctt gatgcagcca
    20341 agctgcgcca acaggcacaa atcgttgcct atgaagccgc cgtccaatcc gcctttcaag
    20401 acgtggcaaa cgcattggcg gcgcgcgagc agctggataa agcctatgac gctttaagca
    20461 aacaaagccg cgcctctaaa gaagcgttgc gtttggtcgg tctgcgttac aaacacggcg
    20521 tatccggcgc gcttgatttg ctcgatgcgg aacgcagcag ctattcggcg gaaggtgcgg
    20581 ctttgtcggc acaactgacc cgcgccgaaa accttgccga tttgtacaag gcactcggcg
    20641 gcggattgaa acgggatacc caaaccgaca aataaccggt cgggcaataa aatgccgtct
    20701 gaaacgataa ggtttcagac ggcatttctt tctttatagt ggattaacaa aaaccagtac
    20761 ggcgttggct cgccttagct taaagagaac gattctctaa ggtgctgaag caccaagttg
    20821 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatat
    20881 gtttgacggt tgcggtcagg ctgcgcggcg tgccgtaaac gtgaatgtcg ggcatagggc
    20941 ggtagcgttt gttgaacacg ttttcaaagt ccaaaccgat gcgggtggat ttgccgattt
    21001 tgtaatgcgc ggtcaaatac agtgtggcgt aggggcgttg ggtcaatgcg gctgcggcgg
    21061 cagggaagaa accgccatac ccgccggcat cccgaagccg aatacggcaa cggcaagcgc
    21121 aatcagcagc gtataaagcc ccgcctgata gcctttcagc ttcaggacgg tcaacgcggc
    21181 aaagaaaaag acgatgggta agagtgcggc ggcggcagtc agatacaggc tgccgccgat
    21241 tgccgtgtag ttttgaaccc aagtttccat aattgaacat ctccgaaaat atttttctaa
    21301 tcgtcggcaa tagtggtcaa accaattaaa gcaacgttgc attactttgc gaaactttaa
    21361 tatttaggtc aatatatttt tgggcggttc ggcagatttg aatcggagct ttcgtttaaa
    21421 tcctgtcaaa acaaatattt gcatgaacaa aaattgtagt ttggtgtagt tttttcttgt
    21481 gtttcggggg cggtgcgagg taaatgccgt tgacggcggg aacggcgtgt cattagaatc
    21541 cgccctgatt ggtcagtcca atttgatgtt tgatgtatag tggatgaaca aaaaccagta
    21601 cggcgttgcc tcgccttagc tcaaagagaa cgattctcta aggtgctgaa gcaccgagtg
    21661 aatcggttcc gtactatttg tactgtctgc ggcttcgtca ccttgtcctg atttttgtta
    21721 atccactata tttcggaagg gttcggcagg gaatctttat aaaggaggag tgatgaaact
    21781 ggtaaggcct cagaaaatca gcgatcaggt attgtcggta ttggaggagc gcattgcgga
    21841 aggggtttac gcggaagggg gcaagattcc gcccgaacgc gttttggcgg aagagttcgg
    21901 cgtttcgcgc ccgtcggtcc ggtcggcatt gaatgttttg gttgcccgtc aggttttgga
    21961 agcgaggcag ggagacggtt attatgtttc ggtcaagccg cagcaggatt ttttgcaaag
    22021 ttggcaggag cttttgggca agcattccaa ttgggaacag gatgtttttg atttcagctg
    22081 ccacatcgag ggctgtatgg cggcattggc ggcagaacgc cggacggatg ccgatttgaa
    22141 gcggattggg ttttggcttg aaaagtttga ggaggcgtgc gaaagcggca atctggaaca
    22201 tcagagcgaa gccgatgtca gcttccatca gacgattgcg gatgcggcgc acaatttgtt
    22261 gttcagccat ttgtcgggcg gtttgttgaa aatgctgtac cggcagacgc gcagcagcct
    22321 tatttatctg aatcaggaag aagatccgcg tccgaagctg atggcgcagc accgtgtatt
    22381 gtatgaggca atatcaaacc gccggccggg cgaggcttcg gaggcggcaa aggcacattt
    22441 gaattatgtg gcgagcagca tcttgaaaga cagggaatat cagagccgta accgccacgc
    22501 agatactttg gcgcaaaatg atttgaagcg cgtgcaggat tgggaggtat gaataccgtt
    22561 tagacggtct ggcatattcg gatgtatcgg tatagtgaac taaatttaaa ccagtacgtc
    22621 gttgcctcgc cttagctcaa agagaacgat tctctaaggt gctgaagcac caagtgaatc
    22681 ggttccgtac tatttgtact gtctgcggct cgccgccttg tcctgatttt tgttaattca
    22741 ctgctgacca atcattatcc gccagataaa gtattctgct tcatccttgt ctatttccag
    22801 caggagaaag ccaaccactt gcttatccca atagattgcc ctcggaaaca catcttcatt
    22861 ttcccggtag agccatgcgt cagctaaaga gcgtacattt ggagccacaa aacgtgctcg
    22921 ttcatcagct tcagatattt tcaaatccag cactgcctga aaactgctct catctactaa
    22981 tttcagctca atcatttttt ctccttttga ataaataggg ttttgaggtg attaaagact
    23041 aatctaactc atataaaagg tagttatcaa aatttatgat tcctttgagg taatctcggt
    23101 ctgctttaga caactcaaat tctggaaaag tcgcaattaa actggacaaa taaaaataat
    23161 tcgggtgcct acgataagaa aaacctgctt gaccgaattt cccttgacaa aaatcatagg
    23221 caagtttcaa ttcttgactt gtgacaccgt ctaaaatttt atctaactct gaaagctgac
    23281 ttttatcagt attttctatc atagctttca ctgtatttac taaaacaaaa agaaaatcat
    23341 tagtattatc cagtaggatt ggtctgttag ataacttatt ctttaactcc atatagtatt
    23401 ggtccataat catttcttcc tctccactcc caaactatcc aacaattctg gatacaaata
    23461 gtatcacccg attcgaacgg gcggataatg aaatgccgtc tgaacagcat ttgcggttcg
    23521 gacggcattt tgtttgcgga gcggcggcgt ttgcttagaa gccttgcgcc agcatcgcat
    23581 cggcaacttt gacgaaaccg gcaatgttcg caccattgac gtagtttact gtgtcgccga
    23641 ctttgccgta tttcagacag gattcgtgga tgctttgcat gatgccgaac aggcgttggt
    23701 cgacttcttc acgagtccaa gacaggcgga tggcgttttg gctcatttcc aaacctgaag
    23761 ttgctacgcc gccggcattg gaggcttttc ccggggcgta gaggatgccg gctttgataa
    23821 attgctcgac cgcgcccaaa gtcgacggca tattcgcacc ttcggcaacg acgtagcagc
    23881 cgtttgccaa cagggttttg gcggcttctt cgtccaattc gttctgggtc gcgcagggca
    23941 gggcgatttc ggcggcgacg ccccacggtt tttggttttc aaagtattgc agaccttgct
    24001 ctttggcgta ggtggcaacg cgttcgcggc ggacttcttt caattcgatc aaggcggcga
    24061 gttgcgcttc ggtcataccg ctgtcgggga agaggacgaa gccgttggag tcggaaacgg
    24121 tcagcacttt cgcgcccagt tggatggctt tttcggcggc gtattgcgcc acgttgccgg
    24181 aaccggaaat caggacgcgt ttgccttcaa aactatcgtt gcgggtttgc agcatcgctt
    24241 gggcgaaata gacgcagccg tagccggtcg cttcgggacg gatgaggctg ccgccccatt
    24301 ccaaaccttt gccggtcagg acggaagaaa actcgttgcg gatttttttg tattgtccga
    24361 acaggtagcc gatttcgcgc ccgcctacgc cgatgtcgcc ggccggaaca tcggtgtccg
    24421 cgccgatgtg gcggtagagt tcggtcataa aggcttggca gaagcgcatt acttcggcat
    24481 cggatttgcc tttggggtcg aagtcggaac cgcctttgcc gccgcccata ggcagggtgg
    24541 tcaaggcgtt tttgaacact tgttcaaaag cgaggaattt caatacgccc aaatcgacgg
    24601 tcggatggaa gcgcaggccg cctttgtaag gaccgatggc ggaactcatt tgcacgcggt
    24661 agccccggtt gacttggact tgccctttat cgtcctgcca ggttacgcgg aacatgacga
    24721 cgcgttcggg ttcgacgatg cgttccagca ggctttgctg ggtgtatttc ggattttttg
    24781 ccaaaaacgg atcgagactc atgaagactt cttcaaccgc ctgatggaac ggctcctgat
    24841 tgggattgcg ttgtttgagg ttggcaaaaa gggtgttcag gtcagtcatt cggatactcc
    24901 ttttggttga tagtgtacta atgggaaaac aaatttgtag gtgggaatat aaacctattt
    24961 ttttctgatg ccaagcccga tttggcaaaa attgttgaca ttattttttc catgttttcg
    25021 atgtttgatg tttgttgccg ataattaatc aataatcata aattaatttc gtaaataaaa
    25081 atattattga aattttattc taagtaatta aactagattt tttgattcca atcaaggtaa
    25141 ttttgttttc ccaccgggtt ttactgtgga aaaggactga aattgccaac aatcctgcct
    25201 gcccgtatct gcgtttttat cgtccggcgg ggcggcgtgt gcgtttgtcc ggactcgggc
    25261 gttggccgga aatgttacaa tatgcgccgt ttaatttgat gaaagactgc tatgaaagcc
    25321 tatttggact tgatgcgcca cgtcctcgac aacggtacgg acaaatcaga ccgcaccggt
    25381 acgggcacgc gctccgtatt cggttatcag atgcgttttg acttgggcaa aggttttccg
    25441 ctgctgacga ccaaaaagct gcatctgcgt tcgattatcc acgagctgct ttggtttctc
    25501 aaaggcgata ccaatatcaa atacctgaag gacaacaatg tttccatttg ggacgagtgg
    25561 gcggacgaaa acggcgactt gggcccggtt tacggctacc agtggcgcaa ctggcccgcg
    25621 cccgacggcc gccatatcga ccaaatcgcc aatgtggtgg aacaaattaa gaaaaacccc
    25681 gactcgcgcc gcctgattgt atcggcgtgg aatccggctt tggtcgatga gatggccttg
    25741 ccgccttgcc acgcgctgtt tcagttttac gttgccgacg gcaaactgtc ctgccagctt
    25801 taccagcgta gtgcagatat tttccttggc gtaccgttca acatcgccag ctacgcattg
    25861 ttgaccatga tgatggcgca agtgtgcgga ttggaagccg gtgagtttat ccatacgttt
    25921 ggcgacgcgc atttgtaccg caaccatttc gagcaggccg cattgcagtt ggaacgcgaa
    25981 ccgcgcgcct tgccagtgat gaaaatcaat cctgaagtca aagatttgtt ttccttcaag
    26041 tttgaagatt tcgagttgga aggttacgac cctcatccgc atatcaaggc ggcagtgtcg
    26101 gtttaggagg cggtatgggc aggcatttcg ggcgcaggcg ttttctgacg gttgccgccg
    26161 ttgcggcggc gggcgcggcg gtttctttcc tgccgaatcc ttttgccgcc gatgatgaaa
    26221 aacgcaacgg ggatgaaaaa cgcaatgaaa atgtgttttt ctggaaaggt gtcgcactgg
    26281 gttccggtgc ggagctccgt ctgttcggtg tggacgacag gcgtgcggcg gatttggtca
    26341 acaaggtttt ggcggaagtg gcgcgtttgg aaaaaattgt tcagccttta ccgtgaagac
    26401 agcctgatca gccgcctgaa cagggacggt tatctgactt cgccgtcggc ggattttttg
    26461 gaactgttga gcctgtgccg cgatattcac gcgctgacgg acggcgcgtt cgatccgaca
    26521 gtgcaggcgt tgtggaaact gtatgccgat tattttgccg cccgtcccga tgcggaaacg
    26581 ccgcctcctc aaaacagcgt ggacgaagcc ctgaaacggg tcggcttcgg caaagtctcg
    26641 tttgacgacc gtgaaatccg ttttgccgaa aagggtatgg gtttgtcctt gaacggcatc
    26701 gcgcaa
//
LOCUS       DAWFIE010000020        26546 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWFIE010000020 DAWFIE010000000
VERSION     DAWFIE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26546
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.020"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(128..463)
                     /locus_tag="WGQ71_000793"
     CDS             complement(128..463)
                     /locus_tag="WGQ71_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0706842.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            592..1497
                     /gene="mtrA"
                     /locus_tag="WGQ71_000794"
     CDS             592..1497
                     /gene="mtrA"
                     /locus_tag="WGQ71_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ0706843.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGNGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESEKPLTGTVS
                     VVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1559..2047)
                     /locus_tag="WGQ71_000795"
     CDS             complement(1559..2047)
                     /locus_tag="WGQ71_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0706844.1"
                     /translation="MKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2194..3096
                     /locus_tag="WGQ71_000796"
     CDS             2194..3096
                     /locus_tag="WGQ71_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0706845.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFTLGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3137..4267)
                     /locus_tag="WGQ71_000797"
     CDS             complement(3137..4267)
                     /locus_tag="WGQ71_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0706846.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPSAENPTSAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4472..7096
                     /gene="alaS"
                     /locus_tag="WGQ71_000798"
     CDS             4472..7096
                     /gene="alaS"
                     /locus_tag="WGQ71_000798"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ0706847.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYVSDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSMD
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGDAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADMARELGIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGEHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELAKAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            7158..7889
                     /locus_tag="WGQ71_000799"
     CDS             7158..7889
                     /locus_tag="WGQ71_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706848.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8196..9089
                     /locus_tag="WGQ71_000800"
     CDS             8196..9089
                     /locus_tag="WGQ71_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0706849.1"
                     /translation="MKTLLQLQTAAQNFAAYYKDQTDERREKDTFWNEFFAIFGIDRK
                     NVAHFEYPVKDPADNTQFVDIFWEGIFLAEHKSANKNLTKAKEQAERYLQEIGRTKPS
                     ALPEYYAVSDFAHFHLYRRVPEEGVENQWQFPLEALPEYITRGVFDFMFGIEAKVRQI
                     QEEADIQAAAAIGRLHDALKEEGIYEEHELRLFITRLLFLFFADDSAVFQRNYLFQDF
                     LESCKETDTLGDKLNQLFEFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTA
                     KQHRALIDCGNFDWRNMRPLQ"
     gene            9255..11195
                     /locus_tag="WGQ71_000801"
                     /pseudo
     CDS             9255..11195
                     /locus_tag="WGQ71_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
     gene            complement(12041..12724)
                     /locus_tag="WGQ71_000802"
     CDS             complement(12041..12724)
                     /locus_tag="WGQ71_000802"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ0706850.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVLEKFYL"
     gene            13021..15324
                     /gene="parC"
                     /locus_tag="WGQ71_000803"
     CDS             13021..15324
                     /gene="parC"
                     /locus_tag="WGQ71_000803"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ0706851.1"
                     /translation="MNMQPHAPHTDSNTLMLGQYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEIHQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPPHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLADMVGRVKAGKVVMTTDSGETVLPPIAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            15344..16861
                     /locus_tag="WGQ71_000804"
     CDS             15344..16861
                     /locus_tag="WGQ71_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ0706852.1"
                     /translation="MVISNTRELEKLKDRIPNLINIARIAIVFPLLIMHIFGLEVSGH
                     ENLHASWTAWAFYVWLAISCWLIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVVCSCLLSYGRYPLLYASYAAILLIFNAIADGDIGKYPHISDAR
                     TASATFILVAASYLSAIFTSLSVRYIDSAGQLARDNHLAYRRIKGLSQTVLERVQEAV
                     VVINTEGLAVLFNRKAKDLFPALEIGRRADLSDSAAELWDQASPHTFEYVLGTLGLTA
                     RIRAVPMNKEPDKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNVCRIDKMLEDISSLNKRNKTERETIGLIPFW
                     EEFKQEFLLGHPDAAGCIRLDMQGNHPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            16913..17380
                     /locus_tag="WGQ71_000805"
     CDS             16913..17380
                     /locus_tag="WGQ71_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEZ0706853.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDSSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            17484..18236
                     /locus_tag="WGQ71_000806"
     CDS             17484..18236
                     /locus_tag="WGQ71_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0706854.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAAHINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
     gene            complement(19233..19439)
                     /locus_tag="WGQ71_000807"
     CDS             complement(19233..19439)
                     /locus_tag="WGQ71_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ0706855.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(19534..20703)
                     /gene="metZ"
                     /locus_tag="WGQ71_000808"
     CDS             complement(19534..20703)
                     /gene="metZ"
                     /locus_tag="WGQ71_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ0706856.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            complement(21002..21763)
                     /locus_tag="WGQ71_000809"
     CDS             complement(21002..21763)
                     /locus_tag="WGQ71_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706857.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            21863..22114
                     /locus_tag="WGQ71_000810"
     CDS             21863..22114
                     /locus_tag="WGQ71_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706858.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            22166..22972
                     /locus_tag="WGQ71_000811"
     CDS             22166..22972
                     /locus_tag="WGQ71_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ0706859.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALKKVRESGEYDKIYAKYFAKENGQAAK"
     gene            23279..24802
                     /locus_tag="WGQ71_000812"
     CDS             23279..24802
                     /locus_tag="WGQ71_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ0706860.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGAATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVES"
     gene            24900..26312
                     /gene="trkA"
                     /locus_tag="WGQ71_000813"
     CDS             24900..26312
                     /gene="trkA"
                     /locus_tag="WGQ71_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ0706861.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         6654 a         7582 c         6439 g         5871 t
ORIGIN      
        1 cttacccctt gtttctcaaa tgccgtctga aataagcggc ttaatatatt gtttacagta
       61 ttgggaagca taacaggcaa aatgccgtct gaaatatttt cagacggcat ttcttatccg
      121 aaacggatta tttttgcgtt tcaaccgctt ccaatgcacg cagggcgtaa gtgtaagcgg
      181 caccggcatt cagggcgatg gcggttgcca atgcgcctgc gatttcgctg tcggtcgcac
      241 cggctttggc ggcggcggcg gcgtgaacac tgatgcagct ctcacaacgt gtggtaatgg
      301 caacggcgat ggcaatcagt tcgcgcgttt tggcatcaag tgcttcggcg gcggcggcct
      361 gttccaatgc gccgtaggcc tgcagcattt taggatgcgc cttacccagc tcgccgaacg
      421 attttttaac caatgcggta tgttctttcc aatctttaaa cattttcttt tcctttctct
      481 tgcgtttaac cctgatacgc gcttgcgtat ctgttttcga tgtgcgtatt attgcaatta
      541 ttcagttgtg tttctcgttt aatcatctca ttttatggtt caaaaagatt tatggacatt
      601 ctagacaaac tggtcgattt cgcccaattg acgggcagtg tggatgtgca gtgccttttg
      661 ggcggacaat ggtcggtacg gcatgaaacc ttgcaacgcg aaggactggt acacattgtt
      721 acatcgggca acggctatct ctgcatcgac ggcgaaactt ccccgcgtcc ggtcagtaca
      781 ggggatattg tatttttccc gcgcggcttg ggtcatgtgt tgagccacga cggaaaatgc
      841 ggagaaagtt tacaaccgga tatgcggcag cacggtgcgt ttacggtcaa acagtgcggc
      901 aacggacagg atatgagcct gttttgcgcc cgtttccgct acgacaccca cgccgatttg
      961 atgaacgggc tgcctgaaac cgtttttctg aacattgccc atccgagttt acagtatgtg
     1021 gtttcaatgc tgcaactgga aagcgaaaaa cctttgacgg ggacggtttc cgtggtcaac
     1081 gcattgtcgt ccgtcctgct ggtgcttatc ctgcgcgcct atctcgaaca ggataaggat
     1141 gtcgaactct cgggcgtatt gaaaggttgg caggacaaac gtttgggaca tttaatccaa
     1201 aaggtgatag acaaaccgga agacgaatgg aatgtcgaca aaatggtggc ggctgccaat
     1261 atgtcgcgcg cgcaactgat gcgccgtttc aaaagccggg tcggactcag cccgcacgcc
     1321 tttgtgaacc atatccgcct gcaaaaaggc gcgttgctgc tgaaaaaaaa cccggattcg
     1381 gttttgtcgg tcgcactgtc ggtaggcttt cagtcggaaa cgcacttcgg caaggcgttc
     1441 aaacggcaat atcacgtttc gccgggtcaa taccggaaag aaggcgggca aaaataaatc
     1501 ggggcttcaa acgcaaatgc cgtctgaaaa ggctttcata cagcatttgc gtaccgcgtc
     1561 atttcaaggg ctgcatcttc atcacttcca tcaaaaagtt ggtaaatgcg gggttgttgg
     1621 gtttgacatc catatttttc caacgctgct gccagccgcg caaggcattc tggatataca
     1681 gcttggactg ttccgtattg attgcgcccc gctggctgtc tatcgccgaa cgcaggtaga
     1741 tttcatacat actgtcatcg acggcattgc gtccgaccag gcgttttctg aagttgttca
     1801 gatattgcgc cgcctgaacc ttggtcattt taccgatacc cacctgatag cccaagcgcg
     1861 tcgcttcatc gctgattttg gcaacatccg tccaatgcga agaggcaagg cggaaacctt
     1921 ttgcaggtgc ttccgttttg acggtattga taggattcac ggggatttcc gtcaatgtgg
     1981 gcacataaat agactggcag ccggaaagaa ctgccgcaat ggaaagaggg ataaggtatt
     2041 ttttcatgcc cccattataa tcaagtttgc cttgagaaaa caaattgttc ggcaagaaaa
     2101 ataaaatttc ggcatcagaa gcaggcaaaa acacattcca caagccttgc cgcaaggttt
     2161 acaatccgac cgtccttatc gcaacgaccg tttatggata ccgcaaaaaa agacatttta
     2221 ggatcgggct ggatgctggt ggcggcggcc tgcttcacca ttatgaacgt attgattaaa
     2281 gaggcatcgg caaaatttac cctcggcagc ggcgaattgg tcttttggcg catgctgttt
     2341 tcaaccgttg cgctcggggc tgccgccgta ttgcgtcggg acaccttccg cacgccccat
     2401 tggaaaaacc acttaaaccg cagtatggtc gggacggggg cgatgctgct gctgttttac
     2461 gcggtaacgc atctgccttt ggccaccggc gttaccctga gttacacctc gtcgattttt
     2521 ttggcggtat tttccttcct gattttgaaa gaacggattt ccgtttacac gcaggcggtg
     2581 ctgctccttg gttttgccgg cgtggtattg ctgcttaatc cctcgttccg cagcggtcag
     2641 gaaacggcgg cactcgccgg gctggcgggc ggcgcgatgt ccggctgggc gtatttgaaa
     2701 gtgcgcgaac tgtctttggc gggcgaaccc ggctggcgcg tcgtgtttta cctttccgtg
     2761 acaggtgtgg cgatgtcgtc ggtttgggcg acgctgaccg gctggcacac cctgtccttt
     2821 ccatcggcag tttatctgtc gtgcatcggc gtgtccgcgc tgattgccca actgtcgatg
     2881 acgcgcgcct acaaagtcgg cgacaaattc acggttgcct cgctttccta tatgaccgtc
     2941 gttttttccg ccctgtctgc cgcatttttt ctgggcgaag agcttttctg gcaggaaata
     3001 ctcggtatgt gcatcatcat cctcagcggt attttgagca gcatccgccc cactgccttc
     3061 aaacagcggc tgcaatccct gttccgccaa agataaaaaa tgccgtccga acatccttca
     3121 gacggcatat cgggctttat ttccccgcct tcacatcctg ccactggcgc accataaact
     3181 tcaatgccgc cggctggata ggcaccatga taaagctgtt tttcaaatcc tcctcggttg
     3241 ggaaaatcgt attgtcgttt ttaaattcgt cttccatcag ctcacgcgcc ggcttgctcg
     3301 aaggcgcata agtaacgaaa ttgccgtttt tcgccgacac ttccgggtcg aggaagtcgt
     3361 tgatgtattt gtgcgcgttg gcgacgtttt tcgcatcttt cggaatcacg aaagaatcca
     3421 cccaaatccc cacgccctct ttgggcatca tcacgcggat tttttccttg ccgcccgctt
     3481 cttcggcacg gcgtttggcg atgttcaaat cgccgccgaa accgattgtt acgcaggtat
     3541 cgccgcgcgc caaatcatcg ataaagccgg acgaagtaaa gcgtttgata ttggggcggt
     3601 ttttcttgag tagggcggtt gcctccctga tgtcttccgt attgctgctg ttcgggtttt
     3661 tacccaaata gttcaacacc ataggataga tttccgccgc gctgtccaaa tagctgatgc
     3721 cgcattgctt gagtttggac gtgtattcgg ggtcgaacac caaatcccac tggttgtccg
     3781 gcagcttgtc cgtacccaaa gcctttttca cgcgttcggt attgatggcg aaggtatttg
     3841 tcccccaata aaacggcacg gcgtattcgt ggccgggatc gaccccgtcc atcagcctca
     3901 tcatttcggg gttgaggtgt ttataattgg gaatcagcga cttatcgatt ttctgatacg
     3961 cacctgcctt aatctgcctg cccacaaacg cattggacgg cgcgacaatg tcgtaaccgg
     4021 acttgcctgt cagcaccttg ctttccagcg tttcatcgct gtcgtacaca tcataagtaa
     4081 ccttgatgcc gtttttcttt tcaaaatcgg caacggtttc cggatcgaca tattccgacc
     4141 agttgtaaat ttttaatacg tttcggtttt ccgccgatgt cggattttcg gcagatggtt
     4201 tgtccgaacc gccgcacgct gcaagcagca aagcagtcag gacggcgagt ggcagatgtt
     4261 tggccattat cattccttgc atatcgggtt ggagaaagcg gtcattatag ccgatattgg
     4321 caacagggct tcagacggca ttcaaaatcc cgccacaccc ttccgaaaac cgccgcttcc
     4381 atagctagaa acagggattt gcggtaagat accgccgttc gttttccctg cttttaccat
     4441 gacaagacat ttgagagaca ttgaaaaaat tatgaaaacc tccgaactgc gccaaaaatt
     4501 cctaaaattt tttgaaacca aaggccacac cgtcgtccgc tcttccagcc tcgtgccgca
     4561 cgacgacccg accctgctgt ttaccaacgc gggcatgaac caatttaaag acgtattctt
     4621 aggtttcgac aaacgcccgt acagccgcgc caccaccgcg caaaaatgcg tacgcgccgg
     4681 cggcaaacac aacgacttgg aaaacgtcgg ctacaccgcc cgccaccaca ccttctttga
     4741 aatgatgggc aacttctcct tcggcgacta cttcaaacgc gacgccatcc atttcgcttg
     4801 ggagtttctg acttcccccg aatggctcaa catccctaaa gacaaactgt tggcgaccgt
     4861 ttacgcggaa gacgacgaag cctacaacat ctggttgaac gaaatcggta tgccgtccga
     4921 gcgcatcgtc cgcatcggcg acaacaaagg cgcgaaatac gtgtccgaca acttctggca
     4981 aatgggcgac accggccctt gcggcccctg ctccgaaatt ttctacgacc acggcgaaga
     5041 aatctggggc ggcattcccg gcagccccga agaagacggc gaccgctgga tcgaaatttg
     5101 gaactgcgta tttatgcagt tcaaccgcga cgaacaaggc aatatgaatc cgctgcccaa
     5161 gccgtccgtc gataccggta tgggcttgga acgtatggcc gccgtcatgc agcatgttca
     5221 cagcaactac gaaatcgact tgttccaaga cctgctcaaa gccgttgccc gcgaaaccgg
     5281 cgcagctttc agcatggatg aacccagcct gaaagtcatc gccgaccaca tccgctcctg
     5341 ctcgttcttg attgcagacg gcgtgttgcc ttccaacgaa ggccgcggct acgtattgcg
     5401 ccgcatcatc cgccgcgccg tgcgccacgg ttacaaactg ggtcaaagca aaccgttctt
     5461 ccacaaactg gtggccgatt tggtcaaaga gatgggcgat gcctaccctg agttgaaaga
     5521 gaaacaagca caaatcgaag aagcgttgaa aaacgaagaa agccgttttg cccaaacgct
     5581 ggaaactggt atggctttgt tggaaaacgc gctggccaaa ggcggcaaaa cactcgacgg
     5641 cgaaatcatc ttcaaactct acgacaccta cggtttcccc tacgacctga ctgccgacat
     5701 ggcgcgcgaa ctgggcatcg aactggacga agcaggcttc gagcgcgaaa tggaagccca
     5761 acgcgcacgc gcacgcgccg cccaaagctt caaagccaac gcccaactgc cttatgacgg
     5821 tcaagacacc gagtttaaag gttatagcga acgccaaacc gaatccaaag tcctcgccct
     5881 ctacaaagac ggcgagcaag tcgacgaatt gaacgaaggc gacagcggcg cagtcgtcat
     5941 cgactttacc ccgttctatg cagaatccgg cggccaagtc ggcgacgtcg gctatatctt
     6001 ctcaggcgaa aaccgctttg aagtacgcga tacccaaaaa atcaaagcag ccgtattcgg
     6061 acaattcggc gtacaaactt caggccgtct gaaagtcggc gacagcgtta ccgccaaagt
     6121 ggacgacgaa atccgcaatg ccaatatgcg caaccacagc gcgacccacc tgatgcacaa
     6181 agccctgcgc gacgtattgg gcgaacacgt cgaacaaaaa ggctctttgg ttaccgccga
     6241 atccacccgt ttcgacattt cccatcccca agcggtaact gccgaagaaa ttgccgaagt
     6301 agaacgccga gtcaacgaag ccatcttggc gaacgttgcc gtcaacgccg ccattatgag
     6361 catggaagat gcacaaaaaa ccggcgcgat gatgcttttc ggcgaaaaat acggcgacga
     6421 agtgcgcgta ctgcaaatgg gcggtttctc aaccgaattg tgcggcggca cgcacgtttc
     6481 acgcaccggc gacatcggcc tcttcaaaat catcagcgaa ggcggtattg ccgcaggcgt
     6541 gcgccgtatc gaagccatca ccggcctgaa cgcactcaaa tgggcgcaag agcaagagcg
     6601 tttggtgaaa gacatcattg ctgaaaccaa agcccaaacc gaaaaagacg tactggcgaa
     6661 aatccaagca ggcgcggcac acgccaaagc cttggaaaaa gagttggcaa aagccaaagc
     6721 cgaactcgcc gtccacgcag gcgcaaaact cttggacgac gcaaaagact tgggcgcagc
     6781 caaactcgtt gctgcccaaa tcgaagccga cgcagccgcc ctgcgcgaaa tcgttaccga
     6841 tttaaccggt aaatccgaca acgccgtgat tcttttagcg gcagtaaacg acggcaaagt
     6901 ctccctgtgc gccggcgtat ccaaaccgtt gaccggcaaa gtgaaagcag gcgatctggt
     6961 taaatttgca gccgaacaag tcggcggcaa aggaggcggc cgtcccgact tggcgcaggc
     7021 cggcggcacg gatgccggca aattgcccga aatgctggta agcgtggaaa gctggctttg
     7081 tcagaaatta agttaaattt aaatcttaat aagccatttg agtcttgttc aaatggctta
     7141 tattttggat tgttagaatg aatactaaaa cagagttaca aaaactttta gaagaggata
     7201 tatcaacttt aacagaaaca ctaatttgtg cagatgctct tcctccaaga tatgtacgta
     7261 gcattgcaac tcctattgtc cgtcgatggt taattgacaa acaattaaat attctcgcaa
     7321 aagaaatagg tttaactata gaattgccca tccttgatac aagtttggta tttgaaaaat
     7381 tgtcaacctt agagaataaa gttaattttt atatggctgg tggagtttat ttaggcggag
     7441 aatttattag ctctatatat cattcatcac aagaattttc tggtgaacca ataatctatg
     7501 ctgaaccaaa tataatttta tgtcctgctg aaaaattttt aactttaaaa cgagttttcc
     7561 ataatggaaa tatttttaat atgaatcaga taattacttt tttatccaat aaacaaggag
     7621 gagtacattt tgataaaaat tacgataaat ataaaacttg gcaagttgct atagaaaaag
     7681 cagcaaattt tttaaaactc ggtaatccat ataatgaaga taaattatct cttagtgaag
     7741 aacatgatac tattttagta gttttaccat tagaaaaagg atatgaatgg aattgtcttg
     7801 agatagaagt actatccgct gctcaatctc tagcaaatat ctattgcaac aaagttcgcc
     7861 tcatagatgg tcatgtttgg aaggaataaa agaaaaagca tagttggtaa tttttagtta
     7921 cgtagggtgg gcttcagccc accgtttttt catttttgat tgttgattgg tgggctaaag
     7981 cccaccctac ggttactaaa atcccgtcat tacctcgcag gcgggaatcc ggtttgttcg
     8041 gtttcggttt tttagggaca gtgccgcaac attaaatttc cagattccca ctttcgcaca
     8101 aataacagcg caaatagggc aaagataagg atatgggttt ttttctttga atttactata
     8161 caattcgccc gtccccgtta tagaaaccca agccgatgaa aaccctgctc caactccaaa
     8221 ccgccgcaca aaacttcgcc gcctactaca aagaccaaac cgacgaacgc cgcgagaaag
     8281 acaccttctg gaacgaattt ttcgccattt tcggcatcga ccgcaaaaac gtcgcccact
     8341 tcgaataccc cgtcaaagac cctgccgaca acacccaatt cgtcgatata ttttgggagg
     8401 gcatcttcct tgccgaacac aaatccgcca acaaaaacct gaccaaggcc aaagagcagg
     8461 cggagcgtta tttgcaggaa atcgggcgca ccaagccctc tgcgctgccc gaatattacg
     8521 ccgtcagcga ttttgcccat ttccaccttt atcgccgcgt acctgaagaa ggcgtggaaa
     8581 accaatggca gttccctttg gaagcattgc ccgaatacat cacgcgcggc gttttcgact
     8641 tcatgttcgg catcgaagcc aaagtccgcc aaattcaaga agaagccgat attcaagcgg
     8701 cggcggccat cggcaggctg cacgacgcgc tcaaagaaga aggcatttac gaagaacacg
     8761 agctgcgcct cttcatcacg cgcctgcttt tcctcttttt tgccgacgac agcgccgttt
     8821 tccagcgcaa ctatctcttt caagactttt tagaaagctg caaagaaacc gacacgctcg
     8881 gcgacaagct caaccaactc tttgaattcc tcaacacacc cgaccaaaag cgcagcaaga
     8941 cccaaagcga aaaatttaaa ggtttcgaat acgtcaacgg cggcctgttt aaagaacgcc
     9001 tgcgcacctt cgacttcacc gccaagcagc accgcgcctt aatcgactgc ggcaatttcg
     9061 actggcgcaa catgagacct ttgcaataac ataggttact aaaattttat gctcaatctc
     9121 attttcaaaa tgcaaaactt ttctgatttt tcctactttt tgctcaatat taggaaggtt
     9181 ttaggcaatt gaaaattttt tggcgcattt ttatgcgtca aatttcgtta acagactatt
     9241 tttgcaaagg tctcaacatc agtcccgaaa tcttcggcac gctcttccaa tcagtcatgg
     9301 acgcacaaga gcggcgcgaa gcgggcgcac actacaccga aaccgccaac atcgacaaag
     9361 tcatcaacgg ccttttttta gaaaacctgc gcgccgaatt tgaagccgtc aaagccctca
     9421 aacgcgacaa agacaaaaaa ctcgccgcct tctaccaaaa aatccaaaac ctgcaattcc
     9481 tcgacccggc ctgcggctgc ggcaacttcc ttatcgtcgc ctacgaccgt atccgcgccc
     9541 tcgaagacga catcatcgcc gaagcgctca aagacaaaac aggcggctta ttcgacagcc
     9601 cgtccgtcca atgccgtctg aaacaatttc acggcatcga aatagacgaa tttgccgtcc
     9661 tcatcgcccg caccgccatg tggctcaaaa accaccaatg caacatccgc acgcaaatcc
     9721 gcttcgacgg cgaagtcgcc tgccatacgc tgccgctcga agacgtcgcc gaaatcatcc
     9781 acgccaacag cctccgcaca ccttggcagg cggcggacta catcttcggc gatccgccct
     9841 ttatcggctc gacctaccaa accaaagagc agaaaaacga cctcgaaagc atctgcggcc
     9901 atatcaaagg ctacggcctg ttggattacg tctgcaactg gtatgtcaaa gccgccggta
     9961 tcatggcgca gcatccccaa gttcagacgg catttgtttc caccaattcc atctgccaag
    10021 gccagcaggt cgaaatcctt tggggcagcc ttttgaagca agacatcgaa atccactttg
    10081 cccaccgcac ctttcaatgg acgagccaag ccgcaggcaa agccgccgtc cactgcatca
    10141 tcgtcggctt ccgccaaaag ccgcaaatgc cgtctgaaaa aaccctctac gactatcccg
    10201 acatcaaagg cgaacccgta aaacacgccg cggccaacat caatccgtat ctgattgacg
    10261 cgcccgatgt gattattgcc aagcgcagcc gtcccgtaca ttgcgaacct gacatgatca
    10321 acggaagcaa accgaccgaa ggcggcaacc ttatcctttc aaccgatgaa aaagaagccc
    10381 tgattgccgc cgaacccttg gcggaacaat acatccgtcc ctttgtcggc gcagaggaat
    10441 ttatcaacgg caaaacccgt tggtgcctgt ggtttcacgg cgtatccgac gttaaacgca
    10501 accacgacct gaaacagatg ccccaagttc aagcccgtat tcaggcagtc aaaaccatgc
    10561 gcgaagccag cagcgacaaa caaactcaaa aagatgcagc aaccccgtgg ctttttcaaa
    10621 aaatccgcca gccttcagac ggcaattttc tgattatccc acgcgtctca tccgaaagcc
    10681 gccgtttcgt ccccatcggt tatctatcat atgaaacagt tgtcagcgat ttggtattta
    10741 cccttccaaa cgccagcctc taccacttcg gcatcctcag ctccaccatg cacaacgcct
    10801 ttatgcgtac cgttgccggc cgtctggaaa gtcgttaccg ctattccaat accgttgtgt
    10861 acaacaactt ccccttcccc gaaagctgcc ggatgccgtc tgaaaacgac cgccaagacc
    10921 cgctccgcgc cgccgtcgaa accgccgccc aagccgtcct cgacgcgcgc ggacaatacc
    10981 gccgagaagc gcgggaagcc ggtttgcccg agccgaccct cgccgaactc tatgcgcccg
    11041 acgcaggcta taccgcccta gacaaagccc acgccgccct cgacaaggca gtcgataaag
    11101 cctacggcta caaaacaggc aaaaacaccg acgacgaagc cgaacgcacc gccttcctgt
    11161 tcgagctgta ccgcaaggcg gcggcaattg cgtagtagtt gcgtagtagt tgcgtagtag
    11221 ttgcgtagta gttgcgtagt agttgcgtag tagttgcgta ggatagttgc gtagggtggg
    11281 cttcagccca ccaactccca ccaactccca ccatcttcac cctaaaaccc cgtcatctcc
    11341 gcaaaagcaa ttgcgtaggg tgggcttcag cccaccgttt ttcttcattt tcggttgttg
    11401 attggcaggc tgaagcccac cctgcggtta caaaaatccc gtcattcccg cgcaggcggg
    11461 aatccagacc tttcggggcg gaaacttatc ggataaaacg atttcccctg catcctggat
    11521 tcccactttc atgggaatga cgggatatag gtttccctac ggacgtgttc agattcccgc
    11581 cttcgcggga atgacggcgg agaagtttct gtctttccga taacttaccg taacttaaaa
    11641 tcccgtcatt accgcaaaag cagttgctac ggttacaaaa atcccgtcat ccccacgaaa
    11701 gcgaaaatcc tgcctgtcgg atttcggttt tttcgggcgt ttcgggaact tataaatcgt
    11761 cattcccgcg caggcgggaa tccagaacgc gggacggcgg caatattcaa aggttgtctg
    11821 aaaattcaga ggttctagat tcccgcctgc gcgggaatga cggcggagcg gtttctgctt
    11881 ttccgataaa tgaccgcaac ctaaaaccca tccttcccac gaaaacagaa aaacaaaaac
    11941 ctaaaatctc gtcgttctcg cgaaagcagt tgctacgttt accgtcaaaa atgccgtctg
    12001 aacgctcaag ccttcagacg gcatttttta tttcaaaccc ttacaggtag aatttttcga
    12061 ggactttcag gttgtcatcc aatttgtaca ccagcggctg accggtcggg atttccaagc
    12121 ccataatgtc ttcgtcggaa atgccctcga tgtgttttgc cagcgcgcgc agggagttgc
    12181 cgtgcgccgc caccaagacg cgtttgccgc tcaaaatcgc gggggcgatt tggtcttccc
    12241 aaaacggcaa tacgcgctcc agcgttactt tcaggttttc gccgtcgggt acgacatcgg
    12301 caggcagatg ggcatagcgg cggtctttgt gtgcggaaaa ctcatcgtct ttgtccaaaa
    12361 gcggcggcag ggtgtcgtag ctgcgccgcc agatgcggac ttgctcgtcg ccgtattttt
    12421 cggcggtttg ttttttgtcc aggccttgca gttggccgta gtggcgttcg ttcagccgcc
    12481 acgttttgat ttgcggtacg aacagttggt cggattcttc caaaacgatg ttgcaggtct
    12541 taatcgcgcg ggtcaggacg gatgtgaagg cgatgtcgaa ctcatagccg ttttctttca
    12601 gttttttacc ggcggcggca gcctcggcaa gcccctgctc gctcagcttc acgtcgcgcc
    12661 agcctgtaaa caggtttttc gcgttccatt cgctttgtcc gtggcggata aataccagtt
    12721 ccatatcgtc tccaatgtgt gaaagtggga aagccttatt tataacatat tttcacattt
    12781 cccgtatttg attcagattc agacacgcgc ccactatggt ttgccgtttt gatttacaat
    12841 aatgtccttt gctttacatt ccgcatacac aatgtttcaa cacacaggac gacacataaa
    12901 gcaccgccct atgtgttgcc ctgatttgga aggggttgcc ccctcccaaa taaagtctga
    12961 ttctaccgcc ccgaaggaca gatgtccaag tggcggggtt tcaaccgaaa aggaaatacg
    13021 ataaatatgc aaccgcacgc tccacatacc gattccaata cgctgatgct cggtcaatac
    13081 gccgaacgcg cctatctcga atacgccatg agcgtggtca aaggccgcgc gctacctgaa
    13141 gtttcagacg gccagaagcc cgtgcagcgg cgcattttgt ttgccatgcg cgatatgggt
    13201 ttgacggcgg gggcgaagcc ggtgaaatcg gcgcgcgtgg tcggcgagat tttgggtaaa
    13261 taccacccgc acggcgacag ttccgcctat gaggcgatgg tgcggatggc gcaggatttt
    13321 accttgcgct atcccttaat cgacggcatc ggcaacttcg gttcgcgcga cggcgacggt
    13381 gcggcggcga tgcgttacac cgaagcgcgg ctgacgccga ttgcggaatt gctgttgtcc
    13441 gaaatccatc aggggacggt ggatttcgtg ccgaactacg acggcgcgtt tgacgaaccg
    13501 ctgcacctgc ctgcacgtct gcctatggtg ttgctcaacg gcgcgtcagg catcgcggta
    13561 ggtatggcga ccgaaattcc gccgcacaat ttgaacgaag ttacgcaggc agcgattgcg
    13621 ctgttgaaaa agccgacgct ggaaaccgcc gacctgatgc aatatattcc tgcgcccgat
    13681 tttgccggcg gcggtcaaat catcacgccg gcggacgaat tgcgccggat ttatgaaacc
    13741 ggcaagggca gcgtgcgcgt gcgtgcgcgt tatgaaatcg aaaaattggc gcgcggacaa
    13801 tggcgcgtca tcgtaaccga gctaccgccg aacgccaatt ccgccaaaat ccttgccgaa
    13861 atcgaagagc agaccaaccc gaaaccgaaa gcgggcaaaa agcagctcaa ccaagaccag
    13921 ctcaatacca aaaagctgat gctggattta atcgaccgcg tgcgcgacga gtccgacggc
    13981 gaacatcccg tgcgactggt gttcgaaccg aaatccagcc gcatcgatac cgataccttc
    14041 atcaacacgc tgatggcgca aacttcgctg gaaggcaatg tgtccatgaa cttggtgatg
    14101 atggggctgg acaaccgccc cgcgcagaaa aacctgaaaa cgattttgca ggaatggctg
    14161 gatttccgca ccgtaaccgt aacacgccgt ctgaaattcc gtttgaacca agtggaaaaa
    14221 cggctgcaca tcctcgaagg ccgtctgaaa gtctttctgc acatcgacga agtgattaaa
    14281 gtcatccgcg aatcagatga cccgaaagcc gatttgatgg cggcgttcgg gctgaccgaa
    14341 atccaagccg aagacatttt ggaaatccgc ctgcgccagt tggcacgttt ggaaggtttc
    14401 aaactcgaaa aagaattgaa cgaattgcgc gaagaacaag gccgtctgaa catccttttg
    14461 ggcgatgaaa acgaaaagcg caagctgatt atcaaagaga tgcaggcgga tatgaaacaa
    14521 tacggcgatg cacgccgcac gctggtggaa gaggccggac gcgccgtgct gacacaaacc
    14581 accgccgacg aacccatcac actgattctg tcggaaaaag gctggatacg cagccgtgcc
    14641 ggacacaatc tcgatttgag ccaaaccgcg ttcaaagaag gcgaccgcct caaacaaacc
    14701 cttgaaggca gaacggtttt acccgtcgtc atcctcgatt cattgggcag aacctataca
    14761 ctcgatgccg ccgaaattcc cggcggacgc ggcgacggcg tgccggtttc atccttgatc
    14821 gagttgcaaa acggcgcgaa acccgttgcg atgttgacag gattgccgga acaacattat
    14881 ttattatcaa gcagcggcgg ctacggcttc atcgccaagt tggctgatat ggtcggacgc
    14941 gtaaaagcgg gcaaagtggt gatgaccaca gacagtggcg aaaccgtcct gccgccgatt
    15001 gccgtctatg cctcctcgtt catcaacccc gactgcaaaa tcattgccgc caccagtcaa
    15061 aaccgcgccc tcgccttccc catcggcgaa ttgaaaatta tggcgaaagg caaagggctg
    15121 caaatcatcg gattaaacgc cggcgaatcg atgacgcata ccgccgtttc ttccgagccg
    15181 gaaatcctga ttgaaagcga aggcaggcgc ggcgcagcgc acaaagaccg catccccatc
    15241 tccctgcttg aggcaaaacg cggcaaaaaa ggcaaactat tacccatatc gggcagcctg
    15301 aaacagcttt cttccccgaa ataaacccga ctctgcacat actatggtaa tttccaatac
    15361 ccgcgaactt gagaaactca aagaccggat tcccaatctg atcaacatcg cccgcatcgc
    15421 catcgtcttc cccctgctga ttatgcatat cttcgggttg gaggtcagcg gacatgaaaa
    15481 cctgcacgct tcgtggacgg cgtgggcatt ttatgtttgg ctcgccattt cctgctggct
    15541 gattttcttt tccatcatcc atccgcattg gcaatggcag tcgctgaaaa tgccgcgttt
    15601 cagcgcggtg gcggacatca cgatgatcgg cgtgctgacc tacctgttcg gcggcatcga
    15661 ttccggcttc ggcatcctga ttctgccctt cgtcgtctgc tcctgcctgc tcagctacgg
    15721 gcgttacccc ctgctctatg ccagctacgc cgccatcctg ctgatattca acgccattgc
    15781 cgacggcgat atcggcaaat atccgcacat atcggatgcc cgaaccgcct cggcaacctt
    15841 catcctcgtc gccgcctcct atctttccgc catcttcacc tcactgtcgg tcagatacat
    15901 cgacagcgcc ggccaactgg cccgcgacaa ccacctcgcc taccggcgca tcaaaggctt
    15961 gagccaaacc gtactcgaac gcgttcaaga agccgtcgtc gtcatcaata ccgaagggct
    16021 ggcggtgctg ttcaaccgga aggcgaaaga ccttttcccc gcactcgaaa tcggacggcg
    16081 cgccgacctg tccgattctg ccgccgaact gtgggaccaa gcctctccgc acactttcga
    16141 atacgtcctc ggcacactcg gcctgaccgc ccgcatccgc gccgtgccga tgaacaaaga
    16201 gccggacaag ctgcttatcc tctacatccg cccgcaaagc gaaattcagg cagaagccct
    16261 gtccgtcaaa cttgccgcgc tcggacaact gaccgccaac cttgcccacg aaatccgcaa
    16321 cccgatgtcc gccatccgcc acgccaacga cctgctgcgc gaaaatatgg aagcgggggc
    16381 ggcagatccg ttcaacgcca aattgtgcaa aatcatcgac ggcaacgtct gccgcatcga
    16441 caaaatgctc gaagacattt cctcactcaa caagcgcaac aaaaccgaac gcgaaaccat
    16501 cggcctgata ccgttttggg aagaattcaa acaagagttc ctgctcggcc atcccgatgc
    16561 cgccggctgc atccgcttgg atatgcaggg caatcacccg accgcctatt tcgatcccgc
    16621 ccacctgcgg cagattatgt ggaatctcgc caacaacgcg tggcggcaca gccgcaaaca
    16681 gcccggctcg atttccgtca ccatccgccc cgcgcaaaaa aacaccgtct gcatcctctt
    16741 tgccgaccgc ccgaagtgca ggaacacctg ttcgaaccct tttacaccac ggcggaaaac
    16801 ggcaccggcc tcgggctgta tgtcgcccgc gaactggcgc acgccaattt cggcgatttg
    16861 acctacctac cggaagccaa atgtttcgaa ctcacattac cggaaaaaac caatgactga
    16921 actgcaacac cccgtcctcg tcgtcgatga cgaaaccgac attctcgacc tgatggaaat
    16981 gaccctgatg aaaatgggct tgcgcgtcca taccgcgtca ggcgttgccg aagccaaaaa
    17041 caagctcgac agccaacgct attcgctcgt cctgaccgat atgcgtatgc cggacagctc
    17101 ggggctggaa gtcgtccaac acatcaacag ccgcctgctc gatacgccgg ttgccgtcat
    17161 caccgccttc ggcaacgccg atcaggcaca ggaagcgttg cgttgcggcg cgttcgaccc
    17221 cgataccatg cagatacagg actatctcga ccaaatcgaa cgcgacatca tcgaacaaac
    17281 cctcaaacaa accgaaggca accgcacgca ggccgccaaa cgcttgggca tcagcttccg
    17341 ttccatgcgc taccgtatgg aacgcctcaa catcggctga cgacaaaacg gcatccgcac
    17401 catctccgcc cacccgaaaa aatgccgtct gaaacggcac gggaaagcgg gttcgcccca
    17461 cgcccgaacg gacacaaaac accatgaccg acatccttat tgacaacacc gccaccgaaa
    17521 ccgtccgcac cctgatacgg gcattccccc ttgtgcccgt ttcccaaccg cccgaacaag
    17581 gcagttacct ccttgccgaa cacgataccg tcagcctcag gcttgtcggg gaaaaaagca
    17641 gcgtcatcgt cgattttgcc tccggcgcgg cacaataccg gcgcacaaaa ggcgggggcg
    17701 aactcatcgc caaagccgtc aaccacaccg cgcaccccac cgtttgggac gcaaccgcag
    17761 gattggggcg cgacagcttc gtcctcgcct cgctcgggct ggccgttacc gccttcgagc
    17821 aacatcccgc cgtcgcctgc ctgctttcag acggcatccg ccgcgccctc ctcaatcccg
    17881 aaacgcaaga caccgccgcg cacatcaacc tccatttcgg caacgccgcc gaacaaatgc
    17941 ccgcccttgt tcaaacacaa ggcaaacccg acatcgtcta tctcgacccc atgtatcccg
    18001 aacgccgcaa aagtgccgcc gttaaaaaag aaatgaccta cttccatcgg ctcgtcggcg
    18061 aagcgcaaga tgaagcggca ctcctgcata ccgcacgcca aacagcaaaa aaacgcgtcg
    18121 tcgtcaaacg cccccgcctc ggcgaacacc ttgccggaca agcccctgcc taccaataca
    18181 ccggcaaaag cacccgcttc gacgtttacc tgccctacgg gacggacaag ggataacgcc
    18241 cataaaacaa gacaccgaaa aatttgccgt tcttatgcaa acgagaaacc ggtttttgcg
    18301 tttcgactgt tttggataaa tcatcacacc ttaaagtttg tcattcccac ggaagtggga
    18361 atccgattca ttcagtttta tagtggttta aatttaaacc actatagctg ttttcgagtt
    18421 tcaggcaact tccaaaccgt cattcccacg gaagtgggaa tctagaaatg aaaggcaaca
    18481 ggaatttatc gtaaatgacc gaaaccgaac ggactagatt cccgcctgcg cgggaatgac
    18541 ggggcgggcg gatgccgtct gaaattccgt cattcccgta aaaacgggaa tctagaactt
    18601 ctgatttttc agacgacttt tgaacattgc cgctacccaa tgatctggat tcccgcctgc
    18661 gcgggaatga cgaggtttca ggttgctgtt tcgggttgct gttttttatg gaaatgacga
    18721 ggttttagat tgcgagaatt tatccgctcc gccgtcattc ccacggaagt gggaatcagg
    18781 aaatgaaaag caataggaat ttatcgtaaa tgaccgaaac cgaacggact agattcccgc
    18841 ctacgcggga atgacgaagt ttcaggttgc tgtttttaag ttgctgtttc gggttgctgt
    18901 tttttatgga aatgacgagg ttttagattg cgagaattta tccgctccgc cgtcattccc
    18961 acggaagtgg gaatccagaa atgaaaagca acaggaattt atcgtaaatg actgaaaccg
    19021 aacggactag attcccgcct gcgcgggaat gacgatgtaa aattatccgg gattcaaaaa
    19081 gacagacttt cgcgtccgtg ggaatgactg cggaaagatg atttttattt tgtcataaaa
    19141 atccgcacct taatcagttg gcggttaaat caaactttta gggtgcagat tactttttat
    19201 gatttcagac agcattttga cagacggcaa acctatttcg gcaataccaa aaacttaatc
    19261 aataactctt tgaacacaaa accgaacacg cccaagccca aaaccaaaaa caaaatggcg
    19321 atgccgaatt tgcccgcttt agactccttg cccaaattcc aaacgataaa acccaaaaaa
    19381 ataatcaaac cggtcaggca gattttcaac gcccaatcgg caaaaaccgc ttcatccata
    19441 tttttttcct attgttgacg tgtatgccat ataagataag ggtttcagac ggcatttgcg
    19501 gtgtttttcc gccgatgccg tctgaacacg caatcagcgc gcgagtgcct gtttcaaatc
    19561 gtcaatcaaa tcgccaacat attccaaacc gaccgacagg cgcaccaatc cggggcggat
    19621 gttggcggcg agtttttctt cgggctgcat cctgccgtgc gtggttgtcc acggatgggt
    19681 gatggtcgag cgcacgtcgc cgaggttggc ggtacgggag aaaagttcga cgccgtccac
    19741 gactttccac gcggctgctt ggtcggcgac ttcaaagccg atgacgatgc cgccgccgtt
    19801 ttgctgtttg cggataagct ccgcctgagg atggtcgggt aatccggtgt aatacacggc
    19861 ttgaacctgc ggctgcgctt gcagccattg cgcgattttc aaggcgttgt cgaactgttt
    19921 ttccatacgc agagacaggg tttccacgcc gctcaacaac tgccatgcgt taaacggcga
    19981 cattgccagc ccgcaagagt tgcagtaaac ggcgacctgc gccatcagtt cttccgaacc
    20041 cgccaacacg ccgcccatca cgcgcccgtg tccgtctatg gctttggtcg cggaggaaac
    20101 ggaaatatcc gcgccgtgtt tcaaaggctg agagccgaca ggcgacagca ggctgttgtc
    20161 caccaccaag agcgcgccga tgccgtgcgc caattccgcc aaggcttcca agtcggccac
    20221 ttcgcccaag ggattggacg gcgtttccaa aaacagcagt ttggtgttgg ctttgacggc
    20281 ggctttccat tcgtttatat cagtcggcga cacgtgactc acttcgatgc cgaatttggt
    20341 aacgatgtta ttgataaagc cgacggtcgt gccgaacagg ctgcggctgg aaatcacatg
    20401 gtcgcccgcc tgcaaaaagg tgaaaaacgc cgcctgaatc gcggacatgc cggtcgaagt
    20461 ggcgaccgcg cgttccgcac cttccaaagc ggcgatgcgt ttttcaaagg cggctgtggt
    20521 cgggttggcg gtacgggtat aagtgaagcc tttgattttt tttgaaaaca aatcggcagc
    20581 gtgttgggcg ttgtcccaca tgaagctgct ggtcaggaac aatgcctgat tgtgttcgcg
    20641 gtattcggtt tgttctttgc cgccgcgtat ggcgagcgtt tgcggatgga gttttttgct
    20701 catcggtgat tcctcggttt tatccgttcg gcaacggagc gtgcgcccgt tgtttaattt
    20761 atagtggatt aacaaaaacc ggtacagcgt tgcctcgcct tagctcaaag agaacgattc
    20821 tctaaggtgc tgaagcacca agtgaatcgg ttccgtacta tttgtactgt cttcggcttc
    20881 gtcgccttgt cctgatttaa atttaatcca ctatattaat attttgcgcc tgttctatga
    20941 tgctttcaag tcgggtgaga atgcaaatgc cgtctgaaac ggctttcaga cggcatggca
    21001 atcagcgttt gtattttaac tcgtacttga tgtcgttgag gattttgcgg acatcgtgtt
    21061 ccaacacgtc ttcgactacc gcacccgcct gctcgtgcag catctgctgg agctgatagg
    21121 taaaaaccgc catctgcttt tgcaccgccg tacggatgat gccgttgacg gtatcggtca
    21181 gatgcgggcg cagacgttta atcagccgtt cggtcagctc ctgttcggac aggcagaaca
    21241 cttcgcgccg gttgacggct ttcggattca ggatattgat ttggacgggc atcaacgcct
    21301 cttccgcacc gccttcgccg ttttccgcaa ccccgtctgc cgccgtaccc gattctgccg
    21361 catcggcgtt ttccccgcct tcaatctgtc cggtttcaaa ttcggcactg tcttttttgg
    21421 catcgaacag gattctccgc cgcgattcga tgtgtttttc cgaaaccgac atttgcaggg
    21481 aagcctgcgc gttgagccag ttttcctgaa ggacgatcat cgggtcggtt tcgacttcct
    21541 cgccgcaatc ggcaacggcg gcattgtgtt cctcctgcca ttttttcaga tacgccttca
    21601 acacacgggc tcggctctca tcgtccagtt tcggcacagg cgcgtccgtt ccggtttcag
    21661 aggggcggga cagcggcgcg taagtcggca ctgccttcat acggcgcgtc tgacgcaggt
    21721 tttccaaacg tttttcccaa ttcggctctt tattcgcatc cattttcggc ttccggttct
    21781 taatctttgc aagcagacaa acccgcgccc aaagcgcggt ttgatataat ggcgcatttt
    21841 aacagattcg cgaggataca tcatgggcag catcgaacag cgtttggaat atctggaaga
    21901 ggcgaacgac gtgctgcgta tgcagaacca cgtcctgtcc accgcattca aagccttaat
    21961 ccgcgccctt cccgccgaaa ccgccgaaat cgcggtcgag tcgattcagc ttgcttttga
    22021 ggacgccttg gcagaattga gctatgagga cagcccccat accgatttgt tccacgacgt
    22081 tacttatgcg tttttccgtg aaaaagaacg ttaattttat gttaaactga ttttttaggc
    22141 tttttgatta ccgaaaggaa ttttgatgaa tatgaaaaaa tggattgccg ccgcccttgc
    22201 ctgttccgcg cttgcgctgt ctgcctgcgg cggtcagggt aaagatgccg ccgcgcccgc
    22261 cgcaaatccc gacaaagtgt accgcgtggc ttccaacgcc gagtttgccc cctttgaatc
    22321 tttagactcg aaaggcaatg tcgaaggttt cgatgtggat ttgatgaacg cgatggcgaa
    22381 ggcgggcaat tttaaaatcg aattcaaaca ccagccgtgg gacagccttt tccccgcctt
    22441 gaacaacggc gatgcggacg ttgtgatgtc gggcgtaacc attaccgacg accgcaaaca
    22501 gtctatggac ttcagcgacc cgtattttga aatcacccaa gtcgtcctcg ttccgaaagg
    22561 caaaaaaata tcttcttccg acgatttgaa aaacatgaac aaagtcggcg tggtaaccgg
    22621 ctacacgggc gatttctccg tttccaaact cttgggcaac gacaatccga aaatcgcgcg
    22681 ctttgaaaac gttcccctga ttatcaaaga actggaaaac ggcggcttgg attccgtggt
    22741 cagcgacagc gcggtcatcg ccaattatgt gaaaaacaat ccgaccaaag gaatggactt
    22801 cgttaccctg cccgacttca ccaccgaaca ctacggcatc gcggtacgca aaggcgacga
    22861 agcaaccgtc aaaatgctga acgatgcgtt gaaaaaagta cgcgaaagcg gcgaatacga
    22921 caaaatctac gccaaatatt ttgcaaaaga aaacggacag gccgcaaaat aagcccgccc
    22981 atccgaacac aatgccgtct gaagcccttt cagacggcat tgttcatcaa tcggcctaca
    23041 atgaactgcc tgctgatttc tccctaccgc aaagcaacag gcaaagatta caaatatcaa
    23101 aatccgagta aaacagtatt ttattaaaac aaattgataa tcaagagatt agaattatgt
    23161 attgtcttta ccgtacaaac gctggcacta tttcaacctg ataaaaaaca gccttcaaaa
    23221 aggttgctta aaacagcagc agacacttac cgccacaacc ttgaaaagga acacaatcat
    23281 gaccgtcatc aaacaggaag actttatcca aagcatttgc gatgccttcc aattcatcag
    23341 ctactaccat cccaaagact acatcgacgc gctctataaa gcgtggcaga aggaagaaaa
    23401 ccccgccgcc aaagacgcga tgacgcagat tttggtcaac agccgcatgt gtgccgaaaa
    23461 caaccgcccc atctgccaag acacaggtat cgcaaccgtg tttttgaaag tcggtatgga
    23521 tgtgcagtgg gatgcggaca tgagcgtgga agaaatggtt aacgaaggcg tgcgccgcgc
    23581 ctacacttgg gaaggcaaca ccctgcgcgc ctctgtcctc gccgatccgg ccggcaaacg
    23641 tcaaaacacc aaagacaaca cgcccgccgt catccacatg agcatcgtgc cgggcggtaa
    23701 agtcgaagta acctgcgcgg caaaaggcgg cggttctgaa aacaaatcca aactcgccat
    23761 gctcaaccct tccgacaaca tcgtcgattg ggtattgaaa accattccga cgatgggcgc
    23821 gggctggtgt cctcccggca tcttgggcat cggcatcggc ggcacgcctg aaaaagccgt
    23881 gttgatggca aaagagtccc tgatgagcca catcgacatc caagaattgc aggaaaaagc
    23941 cgcgtccggc gcagaattat ccaccaccga agccctgcgc ctcgaactct ttgaaaaagt
    24001 caacgcgctg ggcatcggcg cacaaggctt gggcggactg accaccgtgt tggacgtgaa
    24061 aatcctcgat tatccgaccc atgccgcctc caaaccgatt gccatgattc cgaactgcgc
    24121 cgccacccgc cacgtcgagt tcgaattgga cggctcaggc cctgtcgaac tcacgccgcc
    24181 gcgcgtcgaa gactggcccg atttgactta cagccccgac aacggcaaac gcgtcgatgt
    24241 cgacaagctg accaaagaag aagtggcaag ctggaaaacc ggcgacgtat tgctgttgaa
    24301 cggcaaaatc ctcaccggcc gcgatgccgc acacaaacgc ctcgtcgata tgctcaacaa
    24361 aggcgaagaa ttgcccgtcg atttcaccaa ccgcctgatt tactacgttg gtcccgtcga
    24421 tccggtcggc gatgaagtcg tcggcccggc aggccccacc accgccaccc gcatggacaa
    24481 attcacccgc caaatgctcg aacaaaccga cctcttgggc atgatcggca aatccgaacg
    24541 cggcgcggcc acctgcgaag ccatcgccga caacaaagcc gtgtacctga tggcagtcgg
    24601 cggcgcggcg tatctcgtgg caaaagccat caaatcttcc aaagtcttgg cgttccccga
    24661 attgggcatg gaagccattt acgaatttga agtcaaagac atgcccgtaa ccgtcgccgt
    24721 agacagcaaa ggcgaatcca tccacgccac cgccccgccc caatggcagg cgaaaatcgg
    24781 catcatcccc gtcgaatctt gaggcgccat gccgtctgaa cacaaaatct gctttcagac
    24841 ggcatttccg cccctggttg cggtacaatc caccatttca tcactcggcg acccacaccg
    24901 tgaaaatcct cattttaggc aacggacagg taggttctac cgtcgcacaa aaccttgccg
    24961 ccatacccaa caacgacgta accgttatcg acatcgacga aaaagcattg caggaaacag
    25021 gcagccgcct cgatgtccaa accgttttcg gcaacggcgc atcccccttc acattagaac
    25081 gcgccggcgc ggaagatgcc gacttgctgc tcgcgctctc ccgcagcgac gaaaccaaca
    25141 tcgtcgcctg caaagttgcc gccgacctgt tcaacatccc cggccgcatc gcgcgcgtcc
    25201 gttccagcga atacctcgaa tacctcagcc ccaagctcga aaacaacgaa aacggcagcc
    25261 tttccatatt cggcataacc gaaaccatca gccccgaaca gctcgttacc gaacagcttg
    25321 ccggcctgat agactgcccg ggcgcattgc aggttttacg ttttgcagac gaccgcgtgc
    25381 ggatggtcat catacaggcg cggcgcggcg gactgcttgt cggacgcagc attgccgaca
    25441 tcgcccaaga tttgcccgac ggggccgact gccaaatctg cgccgtttac cgcaacaacc
    25501 gcctcatcgt ccccgcgccg caaaccgtca tcatcgaagg cgacgaaatc ctatttgccg
    25561 ccgccgccga aaacatcggc gcggtcatac ccgaattgcg ccccaaagaa accagcaccc
    25621 gccgcatcat gattgccggc ggcggcaaca tcggctaccg cctcgccaaa cagctcgaac
    25681 acgcatacaa cgtcaaaatc atcgaatgtc ggccgcgccg tgccgaatgg atagccgaaa
    25741 acctcgacaa caccctcgtc ctgcaaggtt cggcaaccga cgaaaccctg ctcgacaacg
    25801 aatacatcga cgaaatcgac gtattttgcg ccctgaccaa cgacgacgaa agcaacatta
    25861 tgtccgccct tttggcgaaa aacctcggcg cgaagcgcgt catcggcatc gtcaaccgct
    25921 caagctacgt cgatttgctc gaaggcaaca aaatcgacat cgtcgtctcc ccccacctca
    25981 tcaccatcgg ctcgatactc gcccacatcc ggcgcggcga catcgttgcc gtccacccca
    26041 tccggcgcgg cacggcggaa gccatcgaag tcgtcgcaca cggcgacaaa aaaacttccg
    26101 ccatcatcgg caggcgcatc agcggcatca aatggcccga aggctgccac attgccgccg
    26161 tcgtccgcgc cggaaccggc gaaaccatta tgggacacca taccgaaacc gtcatccaag
    26221 acggcgatca catcatcttt ttcgtctcgc gccggcgcat cctgaacgaa ctggaaaaac
    26281 tcatccaagt caaaatgggc tttttcggat aaaccgcccc attccggaca cattgccgcc
    26341 aagcggtatg gaagcggaat aatggtaggt gggcttcaga cggcatccgc cctccccgtc
    26401 attcccgcgt aagcgggaat ccagaccttg ggatagcggc aatattcaaa ggttatctga
    26461 aaatttagag gttctagatt cccgctttcg cgggaatgac gaaaagttgc gggaatccag
    26521 aacgtcgggc aacggcaata ttcaaa
//
LOCUS       DAWFIE010000021        26446 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWFIE010000021 DAWFIE010000000
VERSION     DAWFIE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26446
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.021"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(192..2954)
                     /gene="gyrA"
                     /locus_tag="WGQ71_000814"
     CDS             complement(192..2954)
                     /gene="gyrA"
                     /locus_tag="WGQ71_000814"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ0706862.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNSAYKKSARIVGDVIGKYHPHGDIAVYDTIVRMAQ
                     DFAMRYVLVDGQGNFGSIDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTINACLRLLDAPETEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVIRAKTHTEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGELVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIRLIKESPNAAEAKEKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRSQGI
                     KAIALKEGDYLVGAAQTGGSDDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     DLEDEAADNENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFSPEAEQSDLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVSETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLECVAEDESESESELSDASVISNVT
                     EPEAEN"
     gene            complement(3052..3552)
                     /gene="hscB"
                     /locus_tag="WGQ71_000815"
     CDS             complement(3052..3552)
                     /gene="hscB"
                     /locus_tag="WGQ71_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ0706863.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            3634..3855
                     /locus_tag="WGQ71_000816"
     CDS             3634..3855
                     /locus_tag="WGQ71_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ0706864.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            complement(3815..4135)
                     /gene="iscA"
                     /locus_tag="WGQ71_000817"
     CDS             complement(3815..4135)
                     /gene="iscA"
                     /locus_tag="WGQ71_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ0706865.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(4222..4608)
                     /gene="iscU"
                     /locus_tag="WGQ71_000818"
     CDS             complement(4222..4608)
                     /gene="iscU"
                     /locus_tag="WGQ71_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ0706866.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            complement(4990..5694)
                     /locus_tag="WGQ71_000819"
     CDS             complement(4990..5694)
                     /locus_tag="WGQ71_000819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706867.1"
                     /translation="MSNKTCFVVMAIGNQHHNGQTITAEDLKARYDNLIKEAILKARP
                     SIEVTRADEVSAMGTITTDIVQRIMYSDYIIADVSYPNPNVFYELGLRHACKPGTIII
                     KDKDAKVPFDIASLRYIEYENSTQGLKDLSNKLLQTFDNFDRDNNRPDNHFLEVAKLT
                     SFKFPEFFKEDPLEVQFMMSLLQSPELIDLFSRQQRGEEVDSSEIMQSLSTHPNILKP
                     VLKYLLQSGELNLLPK"
     gene            complement(5786..7000)
                     /locus_tag="WGQ71_000820"
     CDS             complement(5786..7000)
                     /locus_tag="WGQ71_000820"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ0706868.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISVMWVNNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYVNGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            complement(7029..7475)
                     /gene="iscR"
                     /locus_tag="WGQ71_000821"
     CDS             complement(7029..7475)
                     /gene="iscR"
                     /locus_tag="WGQ71_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ0706869.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            7824..8996
                     /locus_tag="WGQ71_000822"
     CDS             7824..8996
                     /locus_tag="WGQ71_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ0706870.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            complement(9132..11915)
                     /locus_tag="WGQ71_000823"
     CDS             complement(9132..11915)
                     /locus_tag="WGQ71_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ0706871.1"
                     /translation="MANNKTLFEVIENERKAVKKYKPELLEMPEFTSKNLKYDFFEWQ
                     KSALENFLIFDRTSKLDDFPDLKNKPTHLLFNMATGAGKTMMMAALILYYFEKGYRHF
                     LFFVNQNNIVDKTENNFIDPTHAKFLFTEKILQGDTVIPIRKVETFSPHSDGIEIKFT
                     SIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEVELKAN
                     AGNAEIERKGWEHMVLELLLNKNGNPSQNVLLEFTATLPENADVQQKYADKIITKFGL
                     KEFLQKGYTKEINLVSSTLGKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKTI
                     DESKADYLAFLNWAENVQAVDFSFLTTFSTSLNDSDSDNANEQGKTRTEQALKFMQEK
                     GVEFAHLADWVKQNYQKHNVIITNSETNKTKTEKTDSETEKLLNNLEAADNPIRAIFT
                     VDRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFE
                     GKQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLA
                     TFKLKSEFAANKDFRELLIWANKKIPNPNAKTNNANSLQANPQTLSFQVHGNQLLQET
                     QFTADENDEKVKQLGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKL
                     NIQNRNELQNKLLKDWQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFIG
                     TKEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISAR
                     LGDLKSQYDVHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIF
                     IEPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTE
                     SFPLGAASLEKMK"
     gene            complement(11905..13098)
                     /locus_tag="WGQ71_000824"
     CDS             complement(11905..13098)
                     /locus_tag="WGQ71_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0706872.1"
                     /translation="MDPPYNTETDSFAYNDKFNRSTWLTFMKNRLEIARELLADDGAI
                     YINLDYHEVHYCKVLMDEIFGEYNFQREIIWRIGWVSGYKTTVSNYIRNHDTILFYSK
                     DYNHLNFNKYYIDNKHFKVLVDLDRATKNTFTKFGINKETAEKILQEINFSNRPERYP
                     LEDVWNANEYDDLNSIAIVSYSGESVSKMLGLEEIKGQKSEKLLKRILEAHTKPNDIV
                     LDFFGGSGTTAAVAHKMNRQYICIEQIDKQTDTIVKRLNKILVNESSGISKAVNWQGG
                     GEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSINEFSQIIEEPE
                     FQSLPLDEQKQMVLEMLDLNQMYVSLSEMDDEQFADCLNDDDKALSRAFYQSVKNQAE
                     KKDGE"
     gene            complement(13313..13825)
                     /locus_tag="WGQ71_000825"
     CDS             complement(13313..13825)
                     /locus_tag="WGQ71_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706873.1"
                     /translation="MKTDIQTELTQALLPHEKVWANEEKTILAKNILLDLVEKTDPSI
                     IELLLGNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQSVSQSVSQSVSQSVSQSIIHQ
                     INPKETRNLF"
     gene            complement(14029..14952)
                     /gene="truB"
                     /locus_tag="WGQ71_000826"
     CDS             complement(14029..14952)
                     /gene="truB"
                     /locus_tag="WGQ71_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ0706874.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHT
                     GVLDPLATGLLPVCFGEATKFSQYLLDADKAYTATLKLGEASSTGDAEGEIVATARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDI
                     AEFDAPKAVISVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLDETGRDSLLLPCDVLVAHFPQTVLNDYAVHMLQCGQRPRFDEDLTSDTPVRVYT
                     ENGHFVGLAEYQKEICRLKALRLMNTAASAV"
     gene            complement(14985..15356)
                     /gene="rbfA"
                     /locus_tag="WGQ71_000827"
     CDS             complement(14985..15356)
                     /gene="rbfA"
                     /locus_tag="WGQ71_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ0706875.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            15547..16791
                     /gene="clpX"
                     /locus_tag="WGQ71_000828"
     CDS             15547..16791
                     /gene="clpX"
                     /locus_tag="WGQ71_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ0706876.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKGDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(16894..18090)
                     /locus_tag="WGQ71_000829"
     CDS             complement(16894..18090)
                     /locus_tag="WGQ71_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ0706877.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDMAEGLRRLEHALTEFA
                     ATSDNP"
     gene            18494..18994
                     /locus_tag="WGQ71_000830"
     CDS             18494..18994
                     /locus_tag="WGQ71_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706878.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            19079..19633
                     /locus_tag="WGQ71_000831"
     CDS             19079..19633
                     /locus_tag="WGQ71_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706879.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            19647..19817
                     /locus_tag="WGQ71_000832"
     CDS             19647..19817
                     /locus_tag="WGQ71_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706880.1"
                     /translation="MPSEGLSDGIFAFVRGIVMLPFDFQTAFCFPAFDVGMAILIYVR
                     WQGGIVMPPLGF"
     gene            19957..20124
                     /locus_tag="WGQ71_000833"
     CDS             19957..20124
                     /locus_tag="WGQ71_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706881.1"
                     /translation="MNLRKFLIVAKAEKGFYYSLILWVSKPGGAETLQKCRLKLEIGF
                     RRHWFMRQVGA"
     gene            complement(20121..21509)
                     /locus_tag="WGQ71_000834"
     CDS             complement(20121..21509)
                     /locus_tag="WGQ71_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0706882.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEVIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGRGERRYKDR"
     gene            21837..22784
                     /locus_tag="WGQ71_000835"
     CDS             21837..22784
                     /locus_tag="WGQ71_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0706883.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMQPEEYEAWLAEVLEAVVFVTGFAPEQIHLKRRERQKGLQQ
                     YEKTGKTGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHES"
     gene            complement(22837..23169)
                     /gene="trxA"
                     /locus_tag="WGQ71_000836"
     CDS             complement(22837..23169)
                     /gene="trxA"
                     /locus_tag="WGQ71_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ0706884.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGDVVATKVGALAKGQLT
                     AFVEASIA"
     gene            23467..23940
                     /locus_tag="WGQ71_000837"
     CDS             23467..23940
                     /locus_tag="WGQ71_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0706885.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLADMQNELLMHMMKEERMLFPMINQGVGRGAAMPIGVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            complement(24010..24831)
                     /gene="nadE"
                     /locus_tag="WGQ71_000838"
     CDS             complement(24010..24831)
                     /gene="nadE"
                     /locus_tag="WGQ71_000838"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ0706886.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPGQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGTHQTAFANQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            25028..26383
                     /locus_tag="WGQ71_000839"
     CDS             25028..26383
                     /locus_tag="WGQ71_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ0706887.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTAVQGTGSEFQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASISKLAQTLSYSMTQNVRT
                     HTARFERQTQALKHCRPDVSVCKKNIDRFQTALSHAFHQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHIIFADGETDVRVSKEQGQQDLFDC
                     I"
BASE COUNT         6115 a         6350 c         6805 g         7176 t
ORIGIN      
        1 ttttgtttat accggcttag acgacttttt ctcatagggg caactctaac ttaatttgaa
       61 tttccctagt tatctaggac agccccttgt ttttaattga ctataatccg ctatattgtg
      121 agaagctgga tgacggatga gggataaaat aaatgccgtc tgaatcttca gacggcatcg
      181 ggagatgatt ttcagttctc ggcttccggt tcggttacat tggaaattac agaagcgtcg
      241 gagagttcgg attcggattc ggattcgtct tcggcaacac attctagcga tactaaggtt
      301 tcaccttcgt ccaaattgat cagtttcaca cctgcggcgg cgcggccggt ttcgcggatt
      361 tgttcgactt tggtacggat gaggacgccg ccgctggtaa tcagcatcag atcgtcggtt
      421 tcgctgacca aagtcgcggc aaccaaatcg ccgttgcgtt cgccggtatt gatggcgata
      481 ttgccttgtc cgcctttatt tttacggctg taatcggcaa tcggggtacg tttgccgtag
      541 ccgttggcgg tagcggtcaa tacttgcaaa tcgctttgct cggcttcggg ggagaaagta
      601 atcaggctga cgattttgcc gtcggccggc aggcgcatac cgcgcaggcc gccgctgccg
      661 cgaccagacg gacggacacc gtgtttgccg cttggcaggg cattttcgtt gtcggcggct
      721 tcgtcttcga ggtcgtctga aatttcggtt tcgatgtcgg catcttctgc ttcgtcgtta
      781 ccggattttt cccagtattc attgaagcgg atggctttgc ccaagttgga gaacagcatg
      841 atgtcgtccg agccgccggt ttgcgcggcg ccgacgaggt aatcgccttc tttgagtgcg
      901 atggctttga tgccttggct acggacgttt ttaaaggcgg aaagctgaac ttttttcacc
      961 atgccttgtg cagtggcgaa gaagacgtat tggtcttcgg ggaattcgcg cacggcaagg
     1021 atggcgctga ctttttcgcc ttcttccaat tggatgacgt tgttaatcgg acggccgcgg
     1081 ctgttgcgtc cgccttcggg cagtttgtaa accttaatcc agtggcattt gccgaggttg
     1141 gtgaaacaca tcaaataatc atgcgtgttg gcaacaaaca gggtttcgat aaagtcttcg
     1201 tctttggtgg cagctgcctg tttgccgcgc ccgccgcgac gctgagcctg atagtcggtg
     1261 gtcggctgtg ttttgatata gccgccgtgg gtcagggtca cgaccatttc gcgttgcgga
     1321 atcaggtctt catcggcaat gtcgccgccg aacgggttga tttcgctgcg tctttcatca
     1381 ccatagttgg ttttgatttc ttccagttcg tcgcggataa tttgggtaat acgttcgggt
     1441 ttggagagga tgtccacaaa gtcgatgatt ttacccatca ggtttttgta gctttcgaca
     1501 atttcttcct gatcgaggcc ggtcaggttt cgcaggctca tgcgtaaaat agcatctgcc
     1561 tgaatctcgc tcaggtaata accttgctct ttcaagccga tgtttgcagc caatccttcc
     1621 ggacgcatca tttccaaatc cagaccggaa cgcgtcagca tttcttcaac gaggctgctg
     1681 cgccaaggac gcgcaagcag tttttctttg gcctcggctg cgttgggaga ttctttgatg
     1741 agcctgatga tttcatcgat attggacagt gcgacggctt tgccttcggc aatatgccct
     1801 tcatggcgag ccttcttcag ccggaaaagc gtacgtcggg taacgacttc gcggcggtgg
     1861 cgcaggaatt cggagagaat ctgtttcagg ttcaacaggc gcggttgtcc gtcgaccaaa
     1921 actaccatat tgatgccgaa actgtcttgc agcggagtca gtttgtagag ttggtttaag
     1981 acgacttcgg cattttcgtt gcgtttcagc tcgataacga cgcgcatccc agatttgtcg
     2041 gattcgtcgc ggagctcgga aatgccttcc agcgtttttt ctcggaccag ctcaccgatt
     2101 ttctcgacca gtttggcttt gttgacctga taggggattt cgtcgataac gatggcttcg
     2161 cgttccccat ttttgccgat aggttctgtg tgggtcttgg cgcggatgac gacgcggccg
     2221 cggcctgttt tatagccttc gcgcactccg cttaagccgt agatggttgc cccggtcggg
     2281 aagtcggggg cttggataat gtcgatcagt tcgtcgattt cggtttcggg agcatcgagc
     2341 aggcgcaggc aggcattgat ggtatcggaa aggttgtgcg gagggatatt ggtcgccatg
     2401 ccgacggcga tgccggacga gccgttgacg agcagtgtgg ggaaacgggt cggcagtaca
     2461 agcggctcgt gttcgctacc gtcgtagttc gggccgaaat taacggtttc ttcctcaatg
     2521 tctgccagca tttcatgtga gattttcgcc atgcggattt cggtatagcg catggctgcg
     2581 gcggcaagtc cgtctatgga gccgaagttg ccttgaccgt cgaccaagac gtaacgcatg
     2641 gcgaagtctt gtgccatacg gacgatggtg tcatatacgg cgatatcgcc gtgggggtgg
     2701 tatttaccga taacgtcgcc gacaatacgc gccgattttt tgtaggcgga attccagttg
     2761 tttttcagtt cgtgcatggc atacagcacg cggcggtgta ccggtttgag accgtcgcga
     2821 acgtccggca gcgcgcgccc gacaatgacg ctcatggcgt agtcgagata gcttttgcgc
     2881 atttcgtctt caaggcttac cggcagggtt tcgagggcga atttgtggtc gtggcggatg
     2941 gttgcgtcgg tcatggtttc aatgtttcgt atggcaaaaa attgttgctt attttagcat
     3001 attttgacgc ggaacggtgc ggcggttacg ccgtctgaaa cacggtgcgg attataatgc
     3061 cgaggaaatt tcgttgcgga gtttgtcgag aaacctgcct tggcggactt gttgtgcggc
     3121 tgtgtcgtaa tcttgtcggg caaacgactg tttcagaccg cagaacagtt tttcttgttc
     3181 gtcgcggatt tcgttgtcga gatttttcaa ggattcaagg tcgttgcctg cccgtgcctc
     3241 catcagcgtt tcgcgccatt ccatttgctg cataaggaat tcgggggcga aagcggtatg
     3301 ctccggcgcg tcggcatcga tgcccgatgt tttcagcagg taggcggcgc ggtcgatggg
     3361 gtttttcaag gtgcggtagg catcgttgat ggtggaagac atcatcactg cctgcttttg
     3421 ctcaaaggcg gaagctgaag cgaatttatc gggatggaaa cgggcggcca aggcgcggta
     3481 ggtttgttcc aagttttcgg tgtcgatatc gaaagcgggt tcaatccgga agagggtgaa
     3541 atattgggac atagtaggat gataaatgta agattttggc agaaaactgt ttttgcctta
     3601 taatctgccg cttcttaaac gaaaggactg aatatgggcg gcaaagtgca gcacaataaa
     3661 ggcaaaatac gcgacaatgc tttaaaagcc ttagtgaaat ccgatttgtt ccggcacaag
     3721 gtggaacgga aaagaaaagg caaaggcagc tacaacaggc aggaagcgaa aaaatggcgg
     3781 gacggttttg atacggtccc gccattttta tgtcttaaac atggaagctt tcgccgcagc
     3841 cgcaggagtc tttgacattg gggttttcaa atttgaaacc ttcctgcaaa ccttctttgg
     3901 tgtaatcgac ttgtgtgccg tccagataaa ccaggctttt cgggtcgata taaatgcgtg
     3961 cgccgtgtcc ttcaaaaatc aggtcgtcgc catcggcttc atcgacaaat tcaaggttgt
     4021 acgccatccc cgagcagccg ctggttttca cacccaagcg tacgcccaag cctttgccgc
     4081 gtttggcgag atagtcattg atgtgttttg cggcattctc ggtaagggta atcatatttc
     4141 ttccttgttg tatcgccccg gacagacctg aagcggcggg tggttcggac ggcattgcgg
     4201 gacgatgccg tctgaagggc tttatctgtt ttcctgacgt ttgcggtagt cggcaacggc
     4261 cgcttttacc gcatcttcag ccaagatgga gcagtggatt tttaccggcg gcaattccaa
     4321 ctcctcggcg atttcgctgt ttttgattgc cagcgcgtca tccaggcttt tgcctttaac
     4381 ccactcggta atcaggctgg acgaagcgat ggcggaaccg cagccgtaag ttttgaattt
     4441 cgcatcttcg atgatgcctt catcgttcac tttgatttgc aggcgcatca cgtcgccgca
     4501 ggccggtgcg ccgaccatgc cggtgccgac agattcgtcg tttttgtcga aagtaccgac
     4561 gttgcgggga ttttcgtagt ggtcgattac tttatcgctg tatgccatga tgtggtttcc
     4621 ttaatgtttt gatgatttaa gtggtttgtt tgccggtttt cagacggcct gaattagatt
     4681 ttgcacgcgc cgccttcgca gccgtcatca ttctctgaat cgacgccgcc ttccatggta
     4741 acgccgtcaa aatcttcaag caggttgtcg agattgtcga ggtcaagttc ttgttcgttc
     4801 atgggttgca tccttaattt taatgtggtg gcttttaggt cgtctgaaaa ggtcttttgg
     4861 gattttagac gacctttttt gtttttaatg tgtggattga atggggtggg ttgtcaagcc
     4921 taatgtttcg ggtggattga tggacttgtt ggattttcaa cctagtctac attttttatt
     4981 ttctatagtt tatttaggta atagatttag ttccccagat tgcaacaaat attttaaaac
     5041 aggttttaaa atatttggat gagttgagag agattgcatt atttcgctag aatcaacttc
     5101 ttctccacgt tgttgtcttg aaaataaatc aattagctct ggagactgaa gtaatgacat
     5161 catgaactgt acctccaatg ggtcttcttt aaagaattca ggaaatttaa aggaagttaa
     5221 ttttgcaacc tctaggaaat ggttgtctgg tctattatta tctcgatcaa agttatcgaa
     5281 ggtttgcaaa agtttgttag atagatcttt taatccttgt gttgaatttt catattcaat
     5341 atatctcaac gatgcaatat caaaaggaac ttttgcatct ttgtccttaa taatgatagt
     5401 acctggttta cacgcatgcc ttaatccaag ttcataaaac acattaggat taggataact
     5461 aacatccgca ataatataat cagaatacat tattctttgt actatatcag ttgtaattgt
     5521 ccccattgct gaaacttcat cagcacgtgt aacttcgata ctaggacggg cttttaagat
     5581 agcttcctta atcaggttgt catatcttgc ctttaaatct tcagctgtaa tggtttgtcc
     5641 gttatgatgt tgatttccta ttgccataac aacaaaacat gtcttattgg acataattca
     5701 actccccttt ttatttacca gtaataaata atatttccac taaaatttga tttaagcact
     5761 caacctacac aataaagtac attaatcaat gcgccgccca ttcaattgag tttaaatcaa
     5821 tcccgtcttt gaacatttcc cacagcggcg acagttcgcg cagtttgccg attttggatt
     5881 taatcagttc ggcggcgaat tgcacttctt cttcggtggt catgcggccg aaagtgatgc
     5941 gcagggatga gtgcgccagt tcgtcgttgc gaccgagcgc gcgtaggacg tagcttggtt
     6001 cgagactggc agaagtacag gcggagccgc tggatacggc gagttctttc accgccataa
     6061 tcaggctttc gccttcgacg aagttgaagc tgacgttcag gttgttcggg gcgcggtgtt
     6121 cgaggtcgcc gttgacatag acttcttcga tgccttcgat gcctttgagg aagatgtcgc
     6181 gcagtttgcg gtagtgcgcc atatcttgtt cgagttcttc tttggcgatg cggaaggctt
     6241 cgcccatacc gacgatttgg tgggtcggca atgtgccgct gcggaagccg cgttcgtggc
     6301 cgccgccgtg catttgggct tcgaggcgga cgcggggttt gcggcggacg tacagcgcgc
     6361 cgatgccttt ggggccgtac actttgtgcg cggacatcga gagcaggtcg attttggcgg
     6421 cttcgacatc gacaggcact ttgccgcagg cttgggcggc atcgacgtgg aagacgattt
     6481 tacgttcgcg gcagatttcg ccgatggcgg gaatgtcttg caccacgccg atttcgttgt
     6541 ttacccacat tacggaaatc aggatggtgt cgtcgcggat ggcggctttg agttcttcca
     6601 aatcaatcaa accgttttct tgaacgccga ggtaggttac ttcaaaacct tggcgttcga
     6661 gttcgcgcat tgtatctaaa acggctttat gttcggtttt gacggtaatg aggtgtttgc
     6721 ctttggtttt gtagaagttt gccgcgcctt tgatggcgag gttgtcggac tcggtcgcgc
     6781 cgctggtgaa gacgatttct ttggggtcgg cattaatcag ggcagcgatg tcggcgcggg
     6841 ctttttcgac ggcctcttct gcttcccagc cgaatgcgtg gctgttggag gcggggttgc
     6901 cgaaggtttc ggtcagatag ggaatcattt tttcggcaac gcgtttgtca acgggggtag
     6961 tggcggcgta gtcgaggtaa acgggggttt tgacggtcat ggtttgctct ttctttttcg
     7021 ggtgttattt aatggatgtg tgtaaattgg acgacgcggc tgccgtcgcc gttgtttttc
     7081 tgttcgatga tgctttgcag ggtaacgctg ccgaggtagt cgttgatggt tttgtttaaa
     7141 ttctcccaaa ggtcgtgcgt caggcagggc gcgccgtggt ggcagttggc tttgctgccg
     7201 cattgggtcg cgtccagccg gtcttcggcg gcggcgatga tttgggcgat gttgatgcgt
     7261 gccgccggtg cggcgaggat gtagccgccg ccgggtccgc gcagactttc aaccagtccg
     7321 gcgcggcgga gtttgccgaa caattgctcg agataggaga gggatatgtt ttggcgttcg
     7381 ctgatggcac tgagtttgac ggcgccggtt tgcgcgttca tcgccaaatc cagcatagcg
     7441 gtaacggcga aacgcccttt ggtggtcagt ctcatggtgg tggttgcccg tgtcggcttt
     7501 ttatagtggg cggaattgtc caatatctga gtgtttcagt caagtatggg ggagctgttg
     7561 ccgattgttt gtttaaatgg cggtatagtc tgattttgct ttatttgttt ggttttttaa
     7621 atcagttggt tatgtttttt gaggagcggc ggttggggcg ttttgccggc aagcggtttc
     7681 aaaacgattt gatttatttt tgatattgat atagattatc taaatcttag tgatttattt
     7741 gggtattgcc tgaaatgctg tctgaaggct caagcggttt cggacggcat attccatcag
     7801 gctgtcaaac agggaaaaac aagatgaaac gtgatttgag caaaatgacc tgcatcgaag
     7861 acttgcgccg cgttgccaag cgcaaaatgc cgcgtatgtt ttacgattac atcgattcgg
     7921 gttcgtggac ggaaacgact tatcgggaaa atacttcgga tttcaaagac atccgtttcc
     7981 gacaaaaagt gttggtcaat atggaggggc gcagcctcga aaccaaaatg attgggcagg
     8041 atgtgaaaat gccggtggcg attgccccga ctggtttcac gggtatggcg cacgcagacg
     8101 gtgaaatctt ggcggcgcgg gcggcggaga agttcggcat tccgtttacg ttgtccacca
     8161 tgtccatctg ctcgattgaa gacgttgccg agaacacttc cgcaccattt tggtttcagc
     8221 tttatgtgat gcgcgaccgc gagtttatgg aaaacctgat taagcgcgcg aaggatgcca
     8281 aatgttcggc attggtattg accgccgatt tgcaggtttt gggtcaacgc cacaaagaca
     8341 tcaaaaacgg cctgtccgcg ccgccgaaac cgaccatcgc caatttaatc aatctggcga
     8401 ccaaaccgga atggtgcatg aaaatgctga acacggaacg ccgcacgttc cgcaatatcg
     8461 tcggacacgc gaagaacgtc ggcgatttgt cttcgctgtc ttcatggact tccgaacaat
     8521 tcgacccgcg cctgagctgg gacgatgtgg cgcgcattaa agatttgtgg ggcggcaagc
     8581 tgattatcaa aggcattatg gaacccgaag acgcggaaaa agcagcgaaa agcggcgcgg
     8641 acgcattggt cgtttccaac cacggcggcc gacagctcga cgataccgtg tctgccatca
     8701 aagccttgcc cgacatcgtc agcgcagtgg gcagcgacat cgaagtttgg atggacagcg
     8761 gcatccgcag cggtcaggat attttgaagg cgtgggcttt gggcgcgaaa ggtacaatga
     8821 taggtcgggc gtttctgtat ggcttgggcg cgtatggaga agaaggcgta acccgcgcgc
     8881 tggaaatcct atataaggaa atggatatat ctatggcgtt tacggggcac cgcgatattc
     8941 aagatgtcga tgcctcgatt ttaaggggta aagattgggg cagggaaacg gtttagatac
     9001 tgaaaaatac gatgccgtct gaacacaggg ttcggacggc attaaaattt ggctgtattt
     9061 taataatgaa ctatctgaca aaacgccctt ttgtaaagga agttttgtca aacggggttg
     9121 cctgcaatgc actacttcat tttttcaagc gatgccgcac ctaaagggaa tgactccgta
     9181 aattgtccat ttttctgatg atcagtaaaa aacggtaaac cgatcaaacg ataatgcggt
     9241 gtatcttttt gcaggatctt atccatccca tattccacag tgatggcttc taaaaattct
     9301 tctttccatc catcagtttc cactaaatgc tcacctttgg gctcaatgaa aatttggtaa
     9361 tgcaaaaagt cgttcacacc attggaagaa gatttttgtt tatctttcag caataaaata
     9421 aagtccggca taaatccttc gccatcggca aagttattca atttaaacac ttcttcatta
     9481 cggattaaat gaacgtcata ttgagatttc aaatcgccca aacgcgctga aataaattga
     9541 attaacgctt cttccaaacc cgttccggca aaattattca tcacatacca atcattttgc
     9601 gtggcaatag tcgtttttac atcatctttt ttgacccatt tttgttttgg cgtgccgaaa
     9661 atttcccata attttttagg tgtaaattct tttgtaccga taaacggcat atcactttca
     9721 ttcagatgtt tttcaaccat ttccaagatt ttcaggcagc ctgcaagttt atcgtccagg
     9781 cgaacctgtt tgtcttgccc taatcctaaa aattcaattt gccagtcttt caacaattta
     9841 ttttgcaatt cattgcgatt ttgaatattg agtttgcttt gcaggcggtc aaaatggaat
     9901 aaagattgac tatttttccc tttgatatgc agggctttat tgaaaatgtg ccgctccatt
     9961 tcactcattt ttatggtttg agtaaaatta ttttgcgtgc caagttgctt gactttttca
    10021 tcattttcat cggctgtaaa ttgcgtttcc tgcaacagtt gattgccgtg aacttggaat
    10081 gaaagcgttt gcggattggc ttgcaggctg tttgcattat tggttttggc gtttgggttg
    10141 gggatttttt tattcgccca aattaacaat tctctaaaat ccttattagc ggcaaattca
    10201 gatttgagtt taaatgttgc caatacctta tcatcgtctt tttcaggcaa ataaccgtct
    10261 tttcgcaact cgtttttcag ttctgaaata tagcgggatt gctcatcgtg cgtgtaataa
    10321 aacaattctt ccaagatacg caattcgtgt tgcatatcgt tgtcaaattt gcgtttattc
    10381 ggctgtttac cttcaaacgc aaatggaaaa taacgcacgc cacgaccaat caactgcttt
    10441 tccgatacgg tggcggcagc cgttttgcct gattttttat ttgaaccgcc gccgttttgc
    10501 ccttcataca aacgcacaat atcaaacaaa ttcagaacgt cccaaccttc ggttaatctg
    10561 tccaccgtaa aaatggcacg aatcggatta tcagccgctt ccaaattatt cagcaatttt
    10621 tctgtttcgc tgtcggtttt ttcggttttg gttttgttgg tttcggagtt ggtaataatc
    10681 acattgtgtt tttgataatt ttgttttacc caatctgcca aatgtgcaaa ctcaacgcct
    10741 ttttcctgca taaattttag ggcttgttca gtgcgggttt tgccttgttc gttggcgtta
    10801 tcgctatcgc tatcgttcaa gcttgttgaa aatgtagtta aaaacgaaaa atcaaccgcc
    10861 tgcacatttt ctgcccaatt taaaaatgcc agataatccg cttttgattc atcaatcgtc
    10921 ttacttctaa acaacatcac aggcttgaaa ttggcaatgc catatttcaa agcgatttga
    10981 tggcgatacc aagcaaataa taaagcgtgc aacacccgct ctttcttgcc cagcgtactg
    11041 gataccaaat tgatttcttt ggtataacct ttttgcaaaa attcttttaa gccaaatttt
    11101 gtgatgattt tatccgcgta tttttgttgc acgtcagcat tttcaggcag cgtggcggta
    11161 aattccaaca gcacattttg gctgggattg ccatttttat tgagtaacaa ttccaaaacc
    11221 atatgctccc agcctttgcg ttcaatttcg gcattgcctg cattggcttt taattccact
    11281 tccaaatcca actcgccttg ttttttgcct ttggtttgcg cgtttaaatg gtgtgcttca
    11341 tcgcccagca tcacaagatt caattcgtgc aaatccgcca atgtggtttg gttttcccgt
    11401 tcggtgtgaa tatcgttata cagcttttga atgctggtaa atttaatttc aatgccgtct
    11461 gaatgtgggc tgaacgtttc cactttgcga atgggaatta ccgtatcgcc ctgcaaaatc
    11521 ttctcggtaa ataaaaattt tgcgtgtgtc gggtcaataa aattattttc cgttttatcc
    11581 acgatattgt tttgattcac aaaaaacaga aaatgccgat accctttttc aaaataatac
    11641 aaaatcaatg ccgccatcat catcgttttg cctgcacctg ttgccatatt gaacagcaaa
    11701 tgcgttggct tattttttaa atcagggaaa tcgtctagct ttgaagtgcg gtcaaaaatc
    11761 aaaaagtttt caagggcaga tttttgccat tcaaaaaaat catattttaa gtttttggac
    11821 gtaaattctg gcatttcaag taattcaggc ttgtattttt taaccgcttt acgttcattt
    11881 tcaatcactt caaacaacgt tttattattc gccatctttt ttctccgctt gattttttac
    11941 tgattgatag aatgcacggc ttaaggcttt atcatcatcg ttcaggcaat ctgcaaattg
    12001 ttcgtcatcc atttcggata atgaaacata catttgattt aaatccaaca tttcaagcac
    12061 catttgtttt tgttcgtcta atggcaaaga ttgaaattca ggctcttcaa taatttggct
    12121 aaattcattt atgctgacgt tgtatttcaa gaaatagcgt tcgcataaac cttcaaacag
    12181 tgttttgatg ccgtctgaat cttcgcaagc caaaatttgt tgttttgcgg tttcgttaaa
    12241 tggagcaagt tcggcataaa caaactcgcc gccaccttgc caattcacgg ctttggaaat
    12301 cccacttgat tcgtttacta aaatcttgtt taaccgcttt acaatagtgt cagtttgttt
    12361 gtctatttgc tcgatacaga tatattggcg attcatttta tgggcaacgg ctgccgttgt
    12421 accgctacca ccgaaaaaat ccaaaacaat atcgtttggc ttggtatgcg cttctaaaat
    12481 ccgttttaac agtttttcag atttttgtcc tttaatttcc tctaatccca gcatttttga
    12541 tacgctttcg cctgaatagc tgacaattgc aatactattc aaatcatcat attcattcgc
    12601 attccaaaca tcttctaacg gataacgttc gggacggttg ctaaaattaa tttcttgcag
    12661 gattttctcg gcagtttctt tattaatacc gaattttgta aatgtatttt ttgttgccct
    12721 atctaaatct actaatacct taaaatgctt attatcaata taatatttgt taaaattcag
    12781 atgattataa tcttttgaat aaaataaaat ggtgtcatga ttacggatat aattggagac
    12841 agtagtttta tagcctgata cccaaccaat acgccaaata atttcacgct ggaaattgta
    12901 ttcaccaaaa atctcgtcca tcaaaacttt gcaataatgt acttcgtggt aatctaagtt
    12961 tatataaatt gcgccatcat ctgccaataa ttctctagca atctccaacc tgtttttcat
    13021 aaatgtcagc caagtagagc gattaaattt atcgttatat gcaaagctat cggtttctgt
    13081 gttatacggc gggtcaatat aaatcagctt gattttgccc ctaaactgct tggcaagcga
    13141 atgcagggca atcagattat tgcctttgat aatgagattt tcggcgggtg tgccgtctga
    13201 atgtcgtttg atttcgccaa ccgcttgttt gccatctgtg gtgtggcgag agaattttga
    13261 gaatgctttt gcgtcaaaaa gccgatcgat ttcatcaaaa gcaagcgttt gattaaaaaa
    13321 gatttcttgt ctctttcggg ttaatttggt gtataattga ctgactgact gactgactga
    13381 ctgactgact gactgactga ctgactgact gactattatt gcgtttaaaa taaatctctt
    13441 cgccttcttc ggtgctttgt ccgccattca acacacaatc tttaaacgga aaatccaaca
    13501 caatatccga attgtctttc aaaaagcggt tgccgtccgt caaaccaatg cggttggcgt
    13561 attttgtgta ggaattattg acgctgtgtt tgtccaagaa aaaacggaaa tcctgcaatt
    13621 taaacaccag cacaccattc acttccacaa agaaatggcg ttttaactca tcattcccca
    13681 ataacaactc gataatggac gggtcggttt tttccaccaa atccaacagg atgtttttgg
    13741 ctaaaatggt tttttcttcg ttcgcccata ctttttcgtg tggtagtagg gcttgggtta
    13801 attcggtttg aatgtctgtc ttcatcgtta taaatcctca tcagttattt taatgtgcca
    13861 ttcttgtgta acggcaaatg gctttgcaaa taaattctaa taaaatcaat cctttgatgt
    13921 ctttgctgtt tccgggggcg ttcgcaaagt tgcgataagc ggggcgggtg tccgagtgtg
    13981 tcgggatttt gcggaaatat caacatatta ttcaaacaca gctgccgttc agacggcgga
    14041 tgccgccgtg ttcatcaggc gcaaggcttt cagacggcat atttcttttt gatattccgc
    14101 caggccgaca aagtggccgt tttccgtgta aacgcgtacc ggcgtatcgg aagtcaggtc
    14161 ttcatcgaaa cgcggccgtt gcccgcattg gagcatatgg acggcataat cgtttaaaac
    14221 ggtttgggga aaatgggcaa ccaatacgtc gcagggcagc agcaagctgt cgcgtccggt
    14281 ttcgtccaag tttgccaagg cttcgagcgt gtggctttgg gcgatggtaa aaccggcggt
    14341 ttcggtgcgg cgcagggcgg tcaggtgggc gaacgtgccg atgtgtttgg cgatgtcttc
    14401 gctgagggtg cggatatagg tgcctttgct gcaacgcacg cttatcacgg ctttgggcgc
    14461 atcaaattcg gcaatatcga tggcgtaaac ggtaatgtcg cgcgctttgc gttcgatgac
    14521 gatgcctttg cgcgcgtatt cgtacagcgg tttaccttcg tgtttgagcg cggaaaacat
    14581 tggtggcact tggcggatgt tgcctgtcag tgcttggcag gccgtctgaa attcggctaa
    14641 ggaaatatcg gcacgggcgg tggcaacgat ttcgccttcg gcatcacccg tgctgctggc
    14701 ttcgccgagt ttcagcgtgg cggtgtaggc tttgtcggca tccagcaggt attgggagaa
    14761 cttggtcgct tcgccgaagc agacgggcaa aagtccggtt gccaaaggat cgagcacgcc
    14821 ggtatgtccg gctttttcgg cacggaacaa acgccgcgct ttttgcagcg cggtgttgct
    14881 ggaaaggcct tcgggtttgt ccaagagcaa aacaccgttg acggcacgtt tttggggttt
    14941 gcctgtattc atggttcgat gttcggacgg catgggcata tccgtcagtc ttcaaccggt
    15001 ttttccgccg ccacttgatc gataagggcg gacaggttca aaccgcgttc caacgattcg
    15061 tcgtatttga aatgcagctc ggggattttg aacagcttga tgcgtttggc caattcgctg
    15121 cggaggtgtc cgcgcgcgtg ttccagcact tcttccgtaa tttcgcgcgc gtcttggttt
    15181 aaaatggtgt agaacacggt ggcgtggctg taatcgcggg taacttcgac ttcgttgacg
    15241 gtaatgaagc cggcgcgcgg gtctttcagt ccggtacgga cgagttcggc aagctcgcgc
    15301 ataatttgtt ctttgacacg gtcttggcgg gcatagccgc gttggggttt tctcatgaat
    15361 atcctcagat ttcagatttg gtttcagacg gcatttttgc cgccggggcg gttgcgaaag
    15421 ggcgtattat agcctaatcg gcgggatttt aaggtaatac attgataaga acggatatag
    15481 ttattatact tggcaacccg aacggcacgt gccgttttct gtaacgcata cattgtaaag
    15541 aaagccatgt ccaacgaaaa ccgtacctgt tccttttgcg gaaaatccaa atcacacgtc
    15601 aaacatttaa ttgagggcga aaacgccttt atctgtgacg aatgcgtctc aaactgtatt
    15661 gaaatattgc atgaagacgg aaatgacggc acaccttcgg aaagtgccgg aggggagccg
    15721 gaagaatccg gcaagctgcc cacgcccgcc gaaatcgtcg ccaacctcaa cgaccacgtt
    15781 atcggtcagg aacaagccaa gaaagccttg gcggtgtcgg tgtacaacca ttacaaacgc
    15841 ctgcgccacc cgaaagccgg agctaatgtc gaattgtcga aatccaacat cctgcttatc
    15901 ggcccgaccg gatcgggtaa aaccctgctg gcgcaatctt tggcgcgtaa actggatgtg
    15961 ccgttcgtga tggcggatgc aaccacgctg actgaagcag gttatgtcgg cgaagatgtc
    16021 gagcaaatca tcaccaaact gttgggcaaa tgtgatttcg atgttgaaaa agcccagcgc
    16081 ggcattgtct atatcgacga aatcgataaa atttcacgca aaggcgacaa tccgtccatc
    16141 acgcgcgacg tgtccggcga aggtgtgcaa caagccttgc tgaaactgat tgaaggtacg
    16201 gtggcaagtg ttccgcccca aggcggccgc aagcatccga atcaggaatt tatcaacgtc
    16261 gataccacca acatcctgtt tatctgcggc ggcgcgtttg caggcttgga aaaagtcatc
    16321 cgccagcgta ctgaaaaagg cggtatcggt ttcggtgcgt ctgttcacag caaggacgaa
    16381 aatgccgaca ttaccaagct gttcggcatc gtcgaaccgg aagatttgat taaattcggt
    16441 ctgattccgg aattaatcgg acgtttgccc gtgattgcaa cactagaaga actggatgag
    16501 gacgcgctga ttaatatttt gaccgagccg aaaaatgctt tggtcaagca gtatcaagcc
    16561 ttgttcggta tggaaaatgt cgagttggaa tttgaagaag gcgcattgcg ttccatcgcg
    16621 cgtcaggcaa tggagcgcaa aaccggcgcg cgcggcctgc gttccatcgt cgaacgctgc
    16681 ctgctcgata ccatgtaccg cctgcctgat ttgaaaggct tgaaaaaagt ggtggtcggt
    16741 aaggcagtca tcgaagaggg cagggaaccg gaattggtgt tcgagtcctg atggcaaaca
    16801 aaaccgcttc ggaacgttga accacgtttc cgaagcggtt ttttgtttct gatgccgtct
    16861 gaagccttcc cgccttcaga cggcatttga gttttacgga ttgtctgatg tcgcggcaaa
    16921 ttccgtcagc gcgtgttcca aacgccgcaa accttccgcc atatcttcat cgttcaacag
    16981 cagcgaaggc gcgaaacgca atacgtccgc acccgcaacc aaaatcatca cgccgtgttt
    17041 caaggcggcg gcggttattt cggatgcgcg tccgcgataa ggcgcgtcca acacgcagcc
    17101 gagcagcagt cccatcccgc gaacctgtga gaacaagcct gttttcctgc ccaaatccag
    17161 caatgccgtc tgaagtttct gcccctggct gcggacgttg tgcaaggttt ccggcgcgtt
    17221 gatgatgtcg aaggcgcggc tgccgaccgc gcacgccatc gggttgccgc cgaaagtcga
    17281 gccatgcgtg cccggttgga aggcggcggc aatcttttct gtcgccagca tcgcgccgat
    17341 cggaaagccg cagcccaagg cttttgccga actcaaaata tcgggaacaa tgccgtaatg
    17401 ttcataggca aacagcctgc ccgtatgccc catcccggtt tgaacttcgt ccaaaatcaa
    17461 caacgcattg tgccggtcgc acagacggcg cgcggtttgc aaatattccg cagtggcggg
    17521 caggatgccg ctttcgccct gtatcggctc gatgatgacc gcgcaggtct gttcgccgac
    17581 ggcagcttcc agcgcggcaa tatcgttgaa cggaacgtgc gtaatgcctt gcggcagggg
    17641 tgcataatcc ttgctgtatt tcggctggcc gccgacggac acggtaaaca gcgtgcgtcc
    17701 gtggaaactg ttgatacagg cgacgatttc gctttttcct ccgccgaaac ggtcgcgcgc
    17761 atatttcctt gccagcttca acgccgcctc attcgcttcc gcgcccgagt tgcagaaaaa
    17821 aaccttgtcg gcaaaactgt ttgcaaccaa tttttgcgcc aattcctgcg ccggacgcgt
    17881 cgtatagata ttggaaatgt gccacagctt gtgcatctgc gcgtttaaag catcgacaag
    17941 ggcagggtgg cagtgtccca gcgcattgac ggcgataccg cctgaaaaat caatatattc
    18001 acgcccttcc gtatcccaaa cgcggctgcc tgaagcgcgt tcgggaatca tcggggcaaa
    18061 ggcgaaattg ggggtcagat agttttgcat aattgctcct gattgcggtt ttgctcggga
    18121 ttatgcaccg tttttcaaag cagataaagg gaaattgccg acaatatcgg gcagcggtaa
    18181 aaataaaccg aaatattttc aatcggatag gtgaaaaatc aaaaaaattt cgataaaatg
    18241 cttgaggcgc ggcagcgttt tgatttagaa tctccccaat atttttatct tcagacggca
    18301 tttcatcatg caaggcaaaa aatttacaac cgcttattgc tattggtacc acacagacgt
    18361 gcggtacttt tttgcgcttg cttgaaatcc atcccatacg caaaacaggc cgctcgaaaa
    18421 cggggcggct tttttgttac cttcacacag gtcgcccgat tcggcaacac acgacagaat
    18481 caggaaaatg accatgaatg caatgcccca aaccgccgca caaaacgaaa ccctgcacac
    18541 ttgcagcatt attgtcgatg atgcacagga tttttccccc atgcttctcg atgcagggcg
    18601 ggacgacaca cttatcagcg aatcgatgat tccgcaaatc cgcactgttg ccgccctgat
    18661 agccgccgaa cggcacgact tcagccgcag cagccccgcc gagtttaccg atgccgccga
    18721 tttttttgcc gcacggattc tcgtattggg cgtgcgacgc ttccatctgg acgtatcgct
    18781 gcttcagatt ttgaaaaccg ccaacagccg cgcccgccgc tttgccgaaa aacaccgcct
    18841 gtttttcaca cccgcacaag ccgaattgag cctgaacaaa cacaaaaacc gccgcctgct
    18901 gaccgtagaa accgaacacg aagtcgaaaa caaaggcaac ccagtcgcca acagcctcgc
    18961 atttgtccgc aaactgcaca ccctgcccct gtaaacatca cgatttgtcg gcagaatgct
    19021 tgccaaaatt cccgacggcg atacaataaa accgattttc cacccaaaca ggacacacat
    19081 gaaaaaaatc atcgcctccg cgcttatcgc aacattcgca ctcgccgcct gccaagacga
    19141 cacgcaggcg cggctcgaac agcagcagaa acagattgaa gccctgcaac agcagctcgc
    19201 acagcaggca gacgatacgg tttaccaact gactcccgaa gcagtcaaag acaccattcc
    19261 tgccgaagca caggcaaacg gcaacaacgg gcaacccgtt accggtaaag acgggcagca
    19321 gtatatttac gaccaatcga caggaagctg gctgctgcaa agcctggtcg gcgcggcggc
    19381 aggcgcgttt atcggcaacg cgctggcaaa caaattcaca cgggcaggca accaagacag
    19441 tcccgtcgcc cgacgcgcgc gtgcagccta ccatcagtcc gcacatccca atgcgcgcac
    19501 cagcagggat ttgaacacgc gcagcctccg tgcaaaacaa caggcggcgc aggcgcagcg
    19561 ttaccgcccg acaacgcgcc cgcccgccaa ttaccgccgc cccgctatgc gcggtttcgg
    19621 caggaggcgg taaacccggc gtgccaatgc cgtctgaagg gctttcagac ggcatttttg
    19681 catttgttag gggcattgtt atgttgccgt ttgattttca gacggcattt tgtttcccag
    19741 cgtttgatgt cgggatggca attctgattt acgtgcgttg gcagggcggc attgtcatgc
    19801 cgccgttggg tttttagacg gcatcatatc gaaccgtcag accatagggc gagcattctt
    19861 gcccaccgtt gcaagggcgg gatttggtgg gcaggaatgc ccaccctgca tctgaacctt
    19921 tccgcgtcat tctcgcgcag gaaggaattt aggtatttga atttacggaa atttttaatc
    19981 gttgccaaag cggaaaaggg tttttattac agcctaatcc tttgggtatc aaagccgggc
    20041 ggtgctgaaa cattgcaaaa atgccgtctg aaactcgaaa tcggtttcag acggcattgg
    20101 tttatgcggc aggttggggc ttagcggtct ttgtaacggc gttcgcctct gcctgcaaaa
    20161 gaatcgccgg ctttgcgttt gcctttgaag ccttcgccgg tttttttgaa accgtctttc
    20221 ttaaagcctt ctttcttgaa accttcgccg cgcgttttgc cgccgaagcc ttctttgccc
    20281 ggtttatgat cgccgcgccg gccgccggat ttcctatcgc cccagccgcc tttgcctttt
    20341 ggcttaccgc ctgcggattt gcgtttgcgg gtcggttcca tgccttcgat ggtcagttcg
    20401 ggcagtttgc ggttaatgta tttttcgatt ttgtggactt tgacgtattc gttcacttcg
    20461 gcaaacgtaa tcgcaatacc cgtgcggcct gcgcggccgg tgcgcccgat gcggtggacg
    20521 tagtcttccg cctgtttcgg caggtcgtag ttgataacgt gggtaatggt cggtacgtcg
    20581 ataccgcgtg cggcaacgtc ggtggcaacc aaaattttgc agcggccttt gcgcaagtcc
    20641 atcagcgtgc ggttgcgcca gccctgcggc atatcgccgt gcaggcagtt tgcggcgaaa
    20701 cctttttcgt acaattcatc cgcgatgact tcggtcatgg ctttggtgga cgtgaaaatc
    20761 acgcattgat cgatgttggc gtcgcgcagg atgtggtcga gcaggcggtt tttgtggcgc
    20821 atatcgtcgc agtacagcag ctgctcttcg attttgcctt ggtcgtccac gcgttcgact
    20881 tcgatgactt cagggtcttt ggtcagtttg cgcgccagtt tgccgaccgc gccgtcccaa
    20941 gtggcggaga acaacaaagt ctgacggtcg ctcggcgttg cttccacgat ggtttcgatg
    21001 tcgtcgataa agcccatatc caacatacgg tcggcttcgt ccaaaatcag cacttccaaa
    21061 cgttcaaaat caactttgcc gctttgcatc aggtccatca gacggcccgg cgtggcgaca
    21121 atcagatcga ccggtttgct cagggcacgg gtttggtagc cgaaagacgc gccgccgacg
    21181 atgctgacgg tgcggaacca acgcatattt ttggcatacg ccagcgcgtt tttctcgact
    21241 tgagccgcca gttcgcgggt cggggtcaac accaaagcac gcgggccttt gcccggtttt
    21301 tcgctgcgtt tggtcagttt ttgcaaagtc ggtaacagaa aggcggcggt tttgccggag
    21361 ccggtttgcg ccgaagccat aatgtcgcgg ccttccaaag caaacggaat cgcctgcgcc
    21421 tgaatcggcg tcgggctttc gtaaccctcg ctgctgacgg cggacaaaat gtttttatca
    21481 aggttcaaat cggcaaattt aatagacatg gctatccttg gaagacaaca acgcccgcgt
    21541 ccgaaaggtt cagacggcct gaagcaggga agggtaacga tacggggaat gtgaagttac
    21601 agggttgtcg caagcggctt gcttgtggtt tacatcgacg gcaacctgca cagatacgct
    21661 ttgcaaaatc aaagcaacag aagaaacgcg cccgtaaggg cggtttaggt tggaaggcga
    21721 tggcttcgta tgacaagata agcggtggat ggtatagggt ttgcccgaaa cggtcaagca
    21781 atcgggcgtt aattacagta aaattgccgt tttccacttt attccgcctg aaaccaatga
    21841 ccgacatcac accgtttgcc aaccgcttgg gcaaaaacat caaacacctt atgaaatggg
    21901 cgaaacgcaa cggcatcgaa gcctggcgca tttacgaccg cgacattccc caattccctt
    21961 ttgccgccga tgtttacggt gatcgaatcc accttcagga atacgatacc ggctggctga
    22021 tgcagcccga agaatacgaa gcgtggcttg ccgaagtatt ggaagccgtc gttttcgtta
    22081 ccggttttgc gcccgaacaa atccacctca aacgccgcga acgtcaaaaa ggtttgcagc
    22141 aatacgagaa aaccggcaaa accggcgatg attttgtcat caccgaaaac ggccgcaagt
    22201 tttgggtcaa cctcgacaaa tacctcgata ccggcctttt cctcgaccac cgcaacacgc
    22261 gcaaaaaagt cggcgaaacc gcagcgggca aacgcttcct caacctgttt tcctacacag
    22321 gcagcttcac cgtctacgcc gccaccggcg gcgcggcatc cagtgaaacc gtcgatttgt
    22381 ccaacactta tctcgattgg gcgaagcgca attttgaatt gaacggcatc gatacggaac
    22441 ggcacaaaat cgtccgcgcc gacgtgttcc aatatcttca gacggcatat ggggaaggca
    22501 ggcggtttga cctcattgtg atggatccgc ccagtttttc caacagcaag aaaatgtccg
    22561 acatcctcga cattcagcgc gaccacaaaa agctgattga cggcgcggtg aagctgctcg
    22621 cttcagacgg cattttgtac ttttccaaca acctgcgcag cttcgtctta gacgacttgg
    22681 tatcggaaca atacgccgtc aaagacattt ccaaacaatc cgttcccgaa gatttccgca
    22741 acaaaaaaat ccataggtgc tgggaaatcc gccacgaatc ttaagggtgc gaaaaagccg
    22801 ccggaatctt ccggcggctt tttcgattgc gctttatcag gcgatagagg cttcgacaaa
    22861 ggcggtcagc tgaccttttg ccaatgcgcc gactttggtg gcgacgacgt cgccgttttt
    22921 gaacaccatc agggtcggaa tgccgcgcac gccgaaacgg gacggggtgg cttcgttgtc
    22981 gtcgatgttg attttgacta ctttcagacg gccttcaaat tcggcggcga tgtcgtccaa
    23041 aatcggggca atcattttgc aggggccgca ccacggagcc caaaagtcca gcaggacggg
    23101 gaggtcggat ttcaaaacat cttgctcgaa agcggcatcg ccggtgtgta cgattaattc
    23161 gctgctcatt atttttcctt ttcggttggg attggcacga tagcgtgcag gatagggctt
    23221 gggcataaaa atttcaagtg ggcgggcggg ataatagttt ttctgtaccg ccccaatcgc
    23281 ctttgcaaat tctaaaaaac ggttcggaca gtattttatt tctgaaaatt acttttatta
    23341 tcaaaatgta aggaatacgt ttgaggctgt acagaagcgc gcgggcggtg tatgctccgt
    23401 ttgaattgat tcttaaagta tgaatgttca aaaccaaacc cgaaaccctg taaaaggaac
    23461 actgcgatga ctgatttttc cgtttgggag accgccccct ttggtgcaac cgtcgatcat
    23521 atcctgcaac gctaccacaa tgtccaccgc gcacaatttg aagaattggt gccgctggcg
    23581 cagaaagtgg cccaagtcca tgccgatacc tttccggcag aaattgccgg gctgcttgcc
    23641 gatatgcaga acgaactgct gatgcatatg atgaaggagg agcggatgct gtttccgatg
    23701 attaatcagg gtgtcggacg cggtgcggcg atgcccatcg gcgtgatgat gcacgaacac
    23761 gaagaacacg accgcgccat cgcacggctc aaagagctga ccgacaattt ccaaccgcca
    23821 gaaggggctt gcggaagctg gacgcggctt tacgcgctgg cgaaagaaat ggtggaggat
    23881 ttgaacgacc acattcattt ggaaaacgat attttgtttg cccgcgtact cgattcttaa
    23941 gggataggag tcggatattt caatgccgtc tgaaatacgg ttttccgttt cagacggcat
    24001 ttccgtgttt cagcccagca attcgggcgg aatgcggcat acgggaatcg ggttgatttt
    24061 gtgctgcatg gcgcggtgaa gtcgcgtata gatttctaga acttcgcgct gccgcccttc
    24121 aaaatcttcg ggtttgcgcg tgccgtacac gcccattgcc cactccagtt cgggatagct
    24181 tgcgcccatc tgttcttcgt cggtgcgttc cgtatcccac aggccgtcgg tcggcggggc
    24241 tttttgaatc gcctcgtcca cgcccaatgc ttcggcaagc cggtaaacct gcgttttggt
    24301 caggtcggca atcgggctga tgtctacgcc gccgtcgccg tatttggtaa aaaatcccac
    24361 gccgaaatct tcaatcttat tacctgtccc cgtaaccagc agtccgtgta tctgcccgta
    24421 gtagtacagg gtcagcatac gcaggcggct tctggcgttg gcgagggaaa gcggctgatt
    24481 ggcaaatgcc gtctgatgag tgccgacggt ttgctcaaag gtctggaagg tgtcggtcag
    24541 atcgaccgtt tgtgcgctta cattggcata ttgccgttgc agattgcgga tgtgcagcct
    24601 tgcccgctca agctggccgg ggtgttggcg tatcggcata tccagaagca gcgtggtgcg
    24661 gccggtgcgg gcggcgagtg cggagacgac ggcggaatcg atgccgccgg aaacgcccac
    24721 gacaaaccct tttgcatttg cccgggcggc gtattcgtcc aaccatcgga caatatgtgt
    24781 gataactgcc tgcgtatcca tttttcctcc tgtcgaaaat atccgcatca tagcttgtcg
    24841 gcggtgtaat ttgaatagat tgagggcggg ttgatgaata tttttcccat tccccgctta
    24901 aaaaagtcat acgggaaaag gcgggcggca agatggcgcg tgttataata ttgttgacaa
    24961 tttaggcggc ccgatgccgc cttgggcttc aaaacatatt catatttttt attatttgat
    25021 tgattcgatg tctgattttt ttcattctga cgtgttgtcc gtttccgagt tgaacgcatt
    25081 tgccaaaagt attttggaaa accaccttgc cggtttgtgg attgccggcg aagtgtccaa
    25141 cctgacccgt gccgccagcg gacattatta tttctcactc aaagacagcc gcgcgcaggt
    25201 gcgttgcgcg atgttcaaag gtgcggcggc gcgtttggcg aagcctttga aagaaggcga
    25261 tcatatcgag gtatcaggaa aaatcagtat ttacgaggcg cggggcgaat ttcaaattac
    25321 cgtaaacgaa gtacggctca aaggtttggg gcagctttac gaagcctatg agcggttgaa
    25381 ggcgcagttg caggcggaag gcgcgtttgc ggcggaacgc aaaaaaccgt tgcccgtccg
    25441 ccctcaatgt atcggcatcg taaccagttt ggcggcggcg gcattgcgcg atgttgtaac
    25501 caccttgaaa cgccgcgcgc ccgaaattcc cgtcattgtt tatccgaccg ccgttcaagg
    25561 cacaggcagc gagtttcaga ttgcccaagc gattaaaacc gcctcgcaac gcgccgaatg
    25621 cgacgtgttg attgtctgtc gcggcggcgg cagcattgaa gacttgtggg cgtttaacga
    25681 agaaccggtc gtacgcgcca tcgaagcctg cacggttccc gtcgtcagtg gcgtgggaca
    25741 cgaaaccgat tttacgcttg ccgattttgt cgccgacgtg cgcgcgccca cgccgaccgg
    25801 tgcggcggag ctggtcagcc ccaaccgcca agaatcgcta catcgtctcg ctcaggcgca
    25861 aggccgtctg aaaaccgttt tggaacaacg ctatttcgat gccagccaaa aactcgactg
    25921 gctcgcgcgg caaatccgtc atccgcgcca aaaactcgac gaacagcgcg cttcaatcag
    25981 caaactggcg caaacgctgt cttactcgat gacgcaaaac gtccgtaccc acaccgcccg
    26041 tttcgaacgc caaacccaag ccctgaaaca ttgccgtcct gatgtttccg tttgcaaaaa
    26101 aaacatcgac cgctttcaga cggccctgtc gcacgccttc caccaactgc ttacccaccg
    26161 ccgccaaagc ctgactgccc aagccgcatt gctcgaagcc gtttcgccgc agcacattct
    26221 ggagcgcggc ttttccgtcg tcaaaaacac acgcggacaa gtcatccgca acgccgatgt
    26281 tttgaagcag gggcagaagc tgcacattat cttcgccgac ggcgaaaccg acgtgcgcgt
    26341 aagcaaagaa caagggcagc aggatttgtt cgattgcatt taaacccaat cggaaaaacc
    26401 gctcccccgc cgccgtcatt cccgcaaaag cgggagtccg gaagcg
//
LOCUS       DAWFIE010000022        23426 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWFIE010000022 DAWFIE010000000
VERSION     DAWFIE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23426
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.022"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(339..761)
                     /locus_tag="WGQ71_000840"
     CDS             complement(339..761)
                     /locus_tag="WGQ71_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ0706888.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(879..1550)
                     /locus_tag="WGQ71_000841"
     CDS             complement(879..1550)
                     /locus_tag="WGQ71_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ0706889.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1566..2192)
                     /locus_tag="WGQ71_000842"
     CDS             complement(1566..2192)
                     /locus_tag="WGQ71_000842"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ0706890.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERSLEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2299..3117)
                     /locus_tag="WGQ71_000843"
     CDS             complement(2299..3117)
                     /locus_tag="WGQ71_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0706891.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQDDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3376..3816)
                     /locus_tag="WGQ71_000844"
     CDS             complement(3376..3816)
                     /locus_tag="WGQ71_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ0706892.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSEPSVLLACGGNLPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3879..5285)
                     /locus_tag="WGQ71_000845"
     CDS             complement(3879..5285)
                     /locus_tag="WGQ71_000845"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ0706893.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDVHVFEEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHTAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLVAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASRNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5455..6570
                     /gene="lptF"
                     /locus_tag="WGQ71_000846"
     CDS             5455..6570
                     /gene="lptF"
                     /locus_tag="WGQ71_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ0706894.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6567..7637
                     /gene="lptG"
                     /locus_tag="WGQ71_000847"
     CDS             6567..7637
                     /gene="lptG"
                     /locus_tag="WGQ71_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ0706895.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELAVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGIWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7948..10533
                     /gene="acnB"
                     /locus_tag="WGQ71_000848"
     CDS             7948..10533
                     /gene="acnB"
                     /locus_tag="WGQ71_000848"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ0706896.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPSEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10675..11670
                     /locus_tag="WGQ71_000849"
     CDS             10675..11670
                     /locus_tag="WGQ71_000849"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ0706897.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(11739..11840)
                     /locus_tag="WGQ71_000850"
                     /pseudo
     CDS             complement(11739..11840)
                     /locus_tag="WGQ71_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11894..12907)
                     /gene="ilvC"
                     /locus_tag="WGQ71_000851"
     CDS             complement(11894..12907)
                     /gene="ilvC"
                     /locus_tag="WGQ71_000851"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ0706898.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(12987..13280)
                     /locus_tag="WGQ71_000852"
     CDS             complement(12987..13280)
                     /locus_tag="WGQ71_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ0706899.1"
                     /translation="MSNIKIVALVTVKPEYTETLVAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAA"
     gene            complement(13347..13838)
                     /gene="ilvN"
                     /locus_tag="WGQ71_000853"
     CDS             complement(13347..13838)
                     /gene="ilvN"
                     /locus_tag="WGQ71_000853"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ0706900.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(13849..15576)
                     /gene="ilvB"
                     /locus_tag="WGQ71_000854"
     CDS             complement(13849..15576)
                     /gene="ilvB"
                     /locus_tag="WGQ71_000854"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ0706901.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
     gene            complement(16285..16369)
                     /locus_tag="WGQ71_000855"
     tRNA            complement(16285..16369)
                     /locus_tag="WGQ71_000855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16333..16335),aa:Leu,seq:caa)
     gene            16645..17298
                     /locus_tag="WGQ71_000856"
     CDS             16645..17298
                     /locus_tag="WGQ71_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ0706902.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            17377..18030
                     /locus_tag="WGQ71_000857"
     CDS             17377..18030
                     /locus_tag="WGQ71_000857"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ0706903.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            18134..19033
                     /locus_tag="WGQ71_000858"
     CDS             18134..19033
                     /locus_tag="WGQ71_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ0706904.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            19030..20319
                     /gene="hisD"
                     /locus_tag="WGQ71_000859"
     CDS             19030..20319
                     /gene="hisD"
                     /locus_tag="WGQ71_000859"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ0706905.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAALRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            20365..21462
                     /locus_tag="WGQ71_000860"
     CDS             20365..21462
                     /locus_tag="WGQ71_000860"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ0706906.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            21459..22376
                     /gene="hisB"
                     /locus_tag="WGQ71_000861"
     CDS             21459..22376
                     /gene="hisB"
                     /locus_tag="WGQ71_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ0706907.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            22471..23340
                     /locus_tag="WGQ71_000862"
     CDS             22471..23340
                     /locus_tag="WGQ71_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ0706908.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKELVESAGGQFAEAPVSGSVGPATNGTLLILFGGDETVLNPLQKVFSLVGKKTF
                     HFGEVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT         5705 a         6503 c         5828 g         5390 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactataatt caataaatta atatatggct taaaataacg
       61 ggattctcgc ctcccgcccg cccgcagaag caggcgggta tcattttaaa acgcggcatt
      121 taaaatttga ccgaaaattg ttgacaatcc ggaatcaagt ctgcacaata ccccgacaag
      181 tccaagtatt ataaaggctg aataaagagg aaacagcagg cagatatatt cgggaggtgc
      241 agtccgaata tatctgcttt tttatgcgcc tccggattgc ctgccgcacc tttcccttca
      301 gacggcatca gccgtttccc cataatgccg cccgatgcct atttatctgc cccggcaatt
      361 tcaaaactgt gggtaatctt tgccgctttg cccaacataa tcgaagccga acagtatttt
      421 tcggcagaca tctgaacggc gcgctcaatg gccgattctt tcaaatcatg cccgaatact
      481 ttgaaatgga tgtggatttc ggtaaacacg cgcggcgcat cgtccgcccg tttcgccgta
      541 accgtcgcac ggcagtcagt cactttctga cgctgttttt cggcaatcat caccacatcg
      601 atgctcgaac agcccgccac gcccaacagc agcatttcca aagggctggg cccgcgctta
      661 gccttacctt ctgccgccga cccctccata acgacgctgt gcccgccttc cgttgttccg
      721 acaaagcaca ttccgtctat ccattttgat gtaacctgca tggtgtcatt cctgaaaata
      781 gcgttaaaac cgctttgcat atggcgttat tgtaaacaat ttcaagcggc ttatgcagaa
      841 atatggacaa aacggcaaaa aaacgcctga aaatcgattt acggtttggc ggactggcca
      901 ttgatctgca ccgatttgag tttcagcgta taggttttgc cgtcgtcggt atagccgatt
      961 tgtgccggaa tattgttcag ggacggtgcg aagaaataca ttaccgcatc gtcgccgcgc
     1021 cgcacccgat atttgacgac ttcggtttcc acgccgccta tgctgtattt tcctgtaccc
     1081 gccttattca aaccgccgac ggaataaagt tttttgccgt tggtgatttt cagccccggg
     1141 gggagtttcg cgtcatttgc cgccaactgc caggcaagcg tgaacaaatc catagccttg
     1201 gggctttgct cggttttgct ctcgcccgct ttgccgtaag ttacgctgcc gtcggcgaat
     1261 ttggcttccg catacagttt gcccctgcgt atgtctctat agtaggtagg gtgcagggta
     1321 ttgccgacaa ccgtaccgcc ggactcgaaa cggatattgt atagcggcac tttaatcgtc
     1381 gaaacgattt tgtaagcatt gccgctgcgt tcaaatgtca tcgtggcggg aatgccgtag
     1441 ctgccggaat agtgcagcac ggcggattgg ggcagccctg ccgcatacgc gcacggcagg
     1501 gcggcggaca aaatggcggc ggaaaatata tttttaaaag tcttcatcat ttgctcccgc
     1561 ccggtttacg ccgtcaggaa acgggcggca tcggcgtttt ccgaatttct gacgcggttt
     1621 ccctcgataa tcaggcggcc ggcggcaaaa tcggcaacgg ctttcggata gagtttgtgt
     1681 tcgacagcca aaacccgggc ggcaatatcg tctgccgtat caccatcaag tatcggcaca
     1741 atcccttgcg atacaatcgg gccgcaatcc agctcggccg taacaaagtg aatcgtacag
     1801 cctgcaacgc ggcaacccgc ttccaaagag cgttcgtgcg tatgaagccc agtaaatgaa
     1861 ggcaggatgg acggatggat gttcatcagc ctgccttcgt aatgggcgca aaactcgggg
     1921 gtcagaatcc gcataaaacc tgccaaaacc accaagtcgg gttgatatgc gtcgattttt
     1981 tccatcatgg cggtatcgaa ggcaagtcgt gattcaaagt ttttatgatt caggctgtcg
     2041 gtcggaatac cctgaccggc cgcccattgc aaaccggcag ccgtttcgct gttgctcaac
     2101 acggcggcaa tacggacgtt gggaatggcg gcattgacga ttgcctgcat attgctgccg
     2161 cgtccggaaa tcaggatgac gatgtttttc ataatggtgc gcttttgaaa gggatgccat
     2221 ctgaaccgct gtttggtggt ttcagacggc atttgccgta aaaatgcccg aaaacctgtt
     2281 tcgggcatgg attcggactt aatttacttt tttgatgtcg acttgagccg gctgcttggc
     2341 gggcgcgttt tcgggtgcgc cgattttgac cagtttcaca tcaaatacca aagtggcgtt
     2401 cgggccgatt ttgtcgcccg caccctgttc gcggtaagca aggttggacg ggatgtagaa
     2461 cgtggcttcg ccgccttctt tcagaagctg tacgccttcg gtccaacccg gaatcacttg
     2521 gctcaaaggg aaggtgaccg ggccgccgtt ggctttgctg ctgtcgaata ccgtaccgtc
     2581 aatcaggcgg ccttcgtatt ccacggtaac gatgtcgtct ttggtcggct gtttgccttc
     2641 accctgtttg gtgattttgt attgcaggcc ggaagcagtg gtcttcacgc cgtctttggc
     2701 ggcattttct ttcagaaagg cttcgccttt ttctttattg gccttcgcgt ccgccttgtg
     2761 tttttctacg gctttagcct gttgttcctg aaggaatttc atcatgactt cctgagcctg
     2821 ctcttcagtc attttgattt ctttgccgtc atacactgcc tgcatggctt cggtaaagac
     2881 tttcaaatcg atttccgcgc cctgttcctt catttgcttc agggagcgtc cgatgtccac
     2941 gcccatcgca tagcttgcct gctgcatcgt gctgccgatc gaagaggtgt cgtcctgcgc
     3001 ggaagaagcg gcggcaggtt cggatgcaga tgcgggggcg gcttcttttt tgccgcaggc
     3061 ggaaagtgcc aaagcggcgg aaagggtcag tgcgctgatt ttgaaaatgg tgttcatgat
     3121 ggatcttcgc tgtcgataag gtcggaaaaa cgggattata gccgagtttg aatgtttcaa
     3181 cacacaggat gacacataaa gcgtcaatcg tgtgttgccc tgttttggaa gggattgaac
     3241 cttccaaaat aagttttgat tctaccgccc cgagggacag atgtccaagt ggcggggttc
     3301 aaccgataag gaaattttaa tcaaatagaa tcaagcctgt ttaaattttg taaatgcggc
     3361 atttcagacg gcattttatg ccttgccctc catgccgtga tgttcgatgg caaaaccgct
     3421 tcggcggtag gcggtaaagc gttcgcgcgc gtcggcgaga tcttccaagc tgttgccgac
     3481 gatttccaaa acgcgcgcgg gcaagaccgg agcggtgttc caaaaaccgt cagacaggtt
     3541 caaaacggtc atgccttcgg gaattcgggg cagattgccg ccgcaggcaa gcaggacgga
     3601 tggttcagac ggcatggctt cttccgtttc ccaaatttcg tgcggaatga aactctcggc
     3661 ttcgtattgc caaagcattt tgtccaattc ctgaagctgc ccgaacgaat cggaccacac
     3721 cagtatcctg ccgccgtccc gaatcgcacg ggcaatcagg cggcaggtga aaatcggaac
     3781 ttgggcaacg tgcgtgtaaa aggtggcttt cggcatattg tttgaacatt tggtaggata
     3841 atgccgtctg aaaggcttca gacggcattg tggcaaaatt aaagattccg cagatagttc
     3901 agcagcaagg gaacgggacg gccagtcgca cctttttccg caccggattt ccacgccgta
     3961 cccgcgatgt caaggtgcgc ccacgggtag ccttcagtaa agtaagacag gaaggtggcg
     4021 gcggtaatcg tgcccgcgcc gggcgtgccg atattgggga tgtcggcaaa gttggatttg
     4081 agctggtctt tgtaggtttc aaagagcggc agttgccacg ctttgtcgtc cacgttgcgg
     4141 gaggcggcga gcaggctgtc gatcaaatcc tgattgttgc ccatcacgcc gctgacatcg
     4201 tgccccaagg cgacgatgca tgcaccggtc agggtggcga cgtcgatgac ggctttgggt
     4261 ttgaactgtt cggcgtaagt gagcgcgtcg cacaaaatca gacggccttc agcatcggtg
     4321 ttcaacactt cgatggtcag acctttcata cttttcacga catcgcccgg tttatttgcc
     4381 gcgccggaag gcatgttttc acaagtggcg acgacggcga cgaggttaat cggcagttgc
     4441 agcttgacgg cggcgcagaa ggtgctgatg acggttgccg cgccgcacat gtcgaacttc
     4501 atttcgtcca tgttcaggcc gggtttgagg gagatgccgc cggtgtcgaa ggtaatgcct
     4561 ttgccgacca ataccacagg cgcggcttct ttgtcggccg caccaaaata gctcagctcg
     4621 accaaataag ggtcttcgac gctgcctttg gcgaccgacc agaatgaacc catgttttct
     4681 ttaatgtagt ctttttcgat gattttggcg tgtgcgccca gtttttcggc ttcggctttg
     4741 gcggtgcgcg ctaaaaattc gggcgtgcat tcgttgggcg cggcgttacc caaatcgcgg
     4801 cagaggcttt gtccgtaaac ctgtgcttcg gcgacgcgca aggcttcttt gacggcggct
     4861 tcgtgcgcgg tatggaacac ggcagtttca aatttggcgg gcttggcttc ttttttgtag
     4921 cggtcgaaac ggtaggcggc attgccgaac gcaatcgcaa acgcttcggc aacggctgca
     4981 gcctgcgctt cttcaaagac gtgaacgtcc acattgaccg tttcctgatt ttgcgcccat
     5041 ttggcggctt cggcggcggc cttgttcaat gcggcgcggc cggtgctttt cagacagcat
     5101 acggcaacag cctgcaaacc gttgcctgtc gggatttttg tgtcggcaaa attttgacct
     5161 tcttcaagcg aagacaaaag ggcaagggcg gtcggatggt ttaattgcgc ctcttcggtg
     5221 cagacaaata actgtgcgcc tgcctgctgt tcctgcaaga tttcggtttt tgtgctaaat
     5281 tccacgttta ttctcctgat tgagacggtt gtcggtagtt ttcggacggc ctttcgctca
     5341 aaagaccgtc tgaagacggc tggcacgatt gtaccccatt tgaagcaccg tctgaaacct
     5401 tgcgcggaca atccgcctgc gccgaaccgc ttaccgcccc ctgaccgcga ttctatgatt
     5461 tatcaaagaa acctcatcaa agaactctct tttaccgccg tcggcatttt cgtcgtcctc
     5521 ttggcggtat tggtctccac gcaggcaatc aacctgctcg gccgtgccgc cgacgggcgt
     5581 gtcgccatcg atgccgtgtt ggcattggtc ggcttctggg tcatcggtat gacgccgctt
     5641 ttgctggtgt tgaccgcatt tatcagtacg ttgaccgtgt tgacccgcta ctggcgcgac
     5701 agcgaaatgt cggtctggct atcctgcgga ttggcattga aacaatggat acgcccagtg
     5761 atgcagtttg ccgtgccgtt tgccgttttg gttgccgtca tgcagctttg ggtgataccg
     5821 tgggcagagc tacgcagccg cgaatacgct gaaatcctga agcagaagca ggaattgtct
     5881 ttggtggagg caggcgggtt caacagtttg ggcaagcgca acggcagggt ttattttgtc
     5941 gaaaccttcg ataccgaatc cggcatcatg aaaaacctgt tcctgcgcga acaggacaaa
     6001 aacggcggcg acaacatcat cttcgccaaa gaaggtaact tctcgctgaa cgacaacaaa
     6061 cgcacgctcg aattgcgcca cggctaccgt tacagcggca cgcccggacg cgccgactac
     6121 aatcaggttt ccttccaaaa actcaacctg attatcagca ccacgccaaa actcatcgac
     6181 cccgtttccc accgccgtac cattccgacc gcccaactga ttggcagcag caacccgcaa
     6241 catcaggcgg aattgatgtg gcgcatctcg ctgaccgtca gcgtcctcct actctgcctg
     6301 cttgccgtgc cgctttccta tttcaacccg cgcagcggac atacctacaa tatcttgatt
     6361 gccatcggtt tgtttttaat ttaccaaaac gggctgaccc tgctttttga agccgtggaa
     6421 gacggcaaaa tccatttttg gctcggactg ctgcctatgc acatcatcat gttcgtcatc
     6481 gcaatcgtac ttctgcgcgt ccgcagcatg cccagccagc ccttctggca ggcggttggc
     6541 aaaagtctga cattgaaagg cggaaaatga acctgatttc acgttacatc atccgtcaaa
     6601 tggcggttat ggcggtttac gcgctccttg ccttcctcgc tttgtacagc ttttttgaaa
     6661 tcctgtacga aaccggcaac ctcggcaaag gcagttacgg catatgggaa atgctgggct
     6721 acaccgccct caaaatgccc gcccgcgcct acgaactgat gcccctcgcc gtccttatcg
     6781 gcggactggt ctctctcagc cagcttgccg ccggcagcga actggccgtc atcaaagcca
     6841 gcggcatgag caccaaaaag ctgctgttga ttctgtcgca gttcggtttt atttttgcta
     6901 ttgccaccgt cgcgctcggc gaatgggttg cgcccacact gagccaaaaa gccgaaaaca
     6961 tcaaagccgc cgccattaac ggcaaaatca gcaccggcaa taccggcatt tggctcaaag
     7021 aaaaaaacag cattatcaac gtgcgcgaaa tgttgcccga ccataccctg ctgggcatta
     7081 aaatctgggc ccgcaacgat aaaaacgaac tggcagaggc agtggaagcc gattccgccg
     7141 ttttgaacag cgacggcagt tggcagttga aaaacatccg ccgcagcacg cttggcgaag
     7201 acaaagtcga ggtctctatt gcggctgaag aaaactggcc gatttccgtc aaacgcaacc
     7261 tgatggacgt attgctcgtc aaacccgacc aaatgtccgt cggcgaactg accacctaca
     7321 tccgccacct ccaaaacaac agccaaaaca cccgaatcta cgccatcgca tggtggcgca
     7381 aattggttta ccccgccgca gcctgggtga tggcgctcgt cgcctttgcc tttaccccgc
     7441 aaaccacccg ccacggcaat atgggcttaa aactcttcgg cggcatctgt ctcggattgc
     7501 tgttccacct tgccggacgg ctcttcgggt ttaccagcca actctacggc atcccgccct
     7561 tcctcgccgg cgcactgcct accatagcct tcgccttgct cgctgtttgg ctgatacgca
     7621 aacaggaaaa acgttgaacc aatgccgtct gccccccttc ggacggcatt ggtttttatt
     7681 atcatattcc cgcagacaga tagccgttcc ctattatagt ggattaacaa aaaccagtac
     7741 ggcgttgcct cgccttgccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
     7801 tttttgttaa tccactatac attacctgtc ataacagttc catttttgtt aaaactagtc
     7861 tatgatagcg gtacaaatat tgtttacaat atttaacgca aatcatttgc aacccgacaa
     7921 aagaaaaaca gaaaaaggaa caaagagatg ttagaagcct atcgtaaagc cgccgccgag
     7981 cgcgccgccc tcggcattcc cgccctccct ttgaacgcgc agcaaaccgc cgatttggtt
     8041 gagctgctga aaagcccgcc cgcaggcgaa ggcgagttct tggtcgaact gcttgcccac
     8101 cgtgttccgc ccggtgtgga cgatgccgcc aaagtcaaag cctcattcct ggctgccgtt
     8161 gccgaaggca gcgcgtccag cccgctgatc tcccccgaat atgcgaccga actcttaggt
     8221 acgatgctcg gcggttacaa tatccacgcc ttaatcgaac tcttggacga cgacaaactc
     8281 gcgcccattg ccgccaaagg cttgaaacat acgcttctga tgttcgattc cttccacgac
     8341 gttcaggaaa aagccgaaaa aggcaacaaa tacgcgcaag aagttttgca atcttgggca
     8401 gatgccgaat ggttcgcctc acgcgccaaa gttcccgaaa aaattaccgt taccgttttc
     8461 aaagttgacg gcgaaaccaa tacagacgac ctctcccccg cgcccgacgc gtggagtcgt
     8521 cccgatattc cgctgcacgc gctggccatg ctgaaaaacc cgcgcgacgg catcacaccc
     8581 gacaaaccga gcgaagtcgg tccgattaaa ttgttggaag aactcaaagc caaaggccat
     8641 ccagttgctt acgtcggcga cgtggtcggt actggttctt cacgcaaatc cgcgaccaac
     8701 tccgtcattt ggcataccgg cgaagacatt ccgttcgtgc cgaacaaacg cttcggcggc
     8761 gtatgtttgg gcggcaaaat cgcgccgatt ttcttcaata cccaagaaga ttccggcgcg
     8821 ctgccgattg aagtcgatgt atctgctcta aaaatgggcg atgtcgtcga tatcctgcct
     8881 tatgaaggca aaatcgtgaa aaacggcgag actgttgccg agtttgaatt gaaatcacaa
     8941 gtattgctgg acgaagtgca agccggcggc cgtatcaacc tgattatcgg ccgaggtctg
     9001 accgccaaag cgcgcgaagc cctgaaactg cctgcctcta ctgcattccg cctgccgcaa
     9061 gcgcctgccg aaagcaaagc cggtttcacc ttggcgcaaa aaatggtcgg ccgcgcctgc
     9121 ggtctgcccg aaggacaagg cgtgcgtccg ggtacttact gcgaaccgcg tatgacgacg
     9181 gtcggctcgc aagacacgac cggcccgatg acccgcgacg agttgaaaga cttggcttgt
     9241 ttgggcttct ccgccgatat ggtgatgcag tctttctgcc acaccgccgc ctatccgaaa
     9301 cctgtcgatg taaaaaccca taaagaactg cccgccttta tttccacccg tggcggcgtg
     9361 tccctgcgtc cgggcgacgg tgtcatccac tcgtggctca accgcctgct gctgcccgat
     9421 accgtcggca ccggcggcga cagccatacc cgtttcccca tcggtatttc cttccccgcc
     9481 ggctccggct tggttgcctt tgccgccgca acgggcgtaa tgccgctcga tatgcctgag
     9541 tctgtattgg tacgcttcag cggcaaactg caaccgggcg taaccctgcg cgatttggtg
     9601 aacgccattc cgctgtacgc aatcaaacaa ggtttgctga ccgttgccaa agccggtaag
     9661 aaaaacatct tctccggccg catcctcgaa atcgaaggcc tgcctgattt gaaagtggaa
     9721 caagcctttg aattgaccga cgcatccgcc gaacgctccg ccgccggctg taccgtgaag
     9781 ctcaataaag agccgattat cgagtacatg aaatccaacg tcgtgttgat gaaaaacatg
     9841 attgccaacg gctatcaaga tccgcgcact ttggaacgcc gcatcaaagc tatggaaaaa
     9901 tggctggcaa atcccgagtt gctcgaagcg gataaagatg ccgaatacgc cgccgtgatt
     9961 gaaatcaaca tggacgacat caaagagccg attatcgcct gcccgaacga cccggacgac
    10021 gtgtgcttca tgtccgaacg ctccggcacc aaaatcgacg aagtattcat cggttcgtgc
    10081 atgaccaaca tcggccactt ccgcgctgct tccaaacttt tggaaggcaa ggcagacacc
    10141 cccgtccgcc tgtggattgc gccgccgacc aaaatggacg cgaaacaatt gtccgacgaa
    10201 ggacactacg gcgtactcgg acgtgccggc gcgcgtatgg aaatgccggg ttgctcctta
    10261 tgtatgggta atcaggcgca agtacgcgaa ggtgcgaccg ttatgtccac ctccacccgc
    10321 aacttcccga accgtttggg taaaaacacc tttgtttacc tcggttcggc ggaattggca
    10381 gcgatttgct ccaaactggg taaaatcccg accgttgaag aatatcaagc caatatcggc
    10441 atcatcaacg aacagggcga taaaatctac cgctatatga acttcaacga aatcgacagc
    10501 tacaacgaag tagccgagac tgtgaacgtt taatccccgt catccgtatg aagtaaggga
    10561 ttgaccgcaa tgccgtctga acaaccttca gacggcattg caacattccg ctaacccttc
    10621 tttccgcaaa cgctgcaaat acggcgttca cgcccccaca taaaggaaac gacagtgaac
    10681 ctgaaaaacc gccattttct gaaactttta gacttcacgc cggaagaaat caccgcctac
    10741 ctcgaccttg ccgccgaatt gaaagccgcc aaaaaagcag ggcgcgagat tcagcggatg
    10801 aaagggaaaa acatcgccct gatttttgaa aaaacctcta ctcggacgcg ctgcgcgttt
    10861 gaagtcgccg cgcgcgatca aggcgcggga gtgacttatt tagagccgtc cgccagccaa
    10921 atcgggcata aggaaagcat caaagacacc gcccgcgtgt tgggcaggat gtacgatgcc
    10981 atcgaatatc gcggtttcgg tcaggaagtt gttgaagaat tggcgaaata cgcgggcgta
    11041 cccgtgttca acgggctgac caacgagttc catcccacac aaatgcttgc cgacgcactg
    11101 actatgcgcg aacacagcgg caaacctttg aaccaaaccg cgtttgccta cgtcggcgac
    11161 gcgcgttaca acatgggcaa ttccctgctg attttagggg caaaattggg gatggacgtg
    11221 cgtatcggcg caccgcaaag cctgtggccg tctgaaggca ttattgccgc cgcacacgcc
    11281 gccgccaaag aaaccggcgc aaaaattacc ctgaccgaaa acgcgcatga agccgtgaag
    11341 aatgttgatt ttattcatac cgatgtgtgg gtcagcatgg gcgagccgaa agaagtctgg
    11401 caggaacgca tcgatttgct gaaagattac cgcgttacgc ccgaactgat ggcggcatcg
    11461 ggcaatccgc aagtcaaatt catgcactgc ctgcccgcct tccacaaccg cgaaaccaaa
    11521 gtcggcgaat ggatttacga aaccttcggg ctgaacggtg tggaagttac agaagaagta
    11581 ttcgaaagcc ccgccagcat cgtgttcgat caggcggaaa accgtatgca cacgattaaa
    11641 gcggtaatgg tcgcggcttt gggcgactga cagaactgtg cctgtttaaa ttcatccgca
    11701 acacagatac cgtctgaaca cgatgttcag acggtatcca tatatagtgg attaaattta
    11761 aaccagtacg gcgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc
    11821 ttgtcctgat ttttgttaat ccactataaa aaaactgcct acaccatgtg taggcagtcc
    11881 cgtttgaaaa caatcagttt ttgtcttggt caaccaattt gttggcagta atccaaggca
    11941 tcatggcacg cagttgtgcg ccgacttttt caacttggtg gtcggcattc agacggcggc
    12001 gggcagtcat agacgcgtag ttgacattac cctcttggat aaacattttt gcgtattcgc
    12061 cggtttgaat gcgtttcagg gcattgcgca tggcttcttt gctggaagca ttgaccactt
    12121 cagggccggt aacgtattcg ccgtactccg cattgttgga aatggagtag ttcatattgg
    12181 caataccgcc ttcgaaaatc aggtcaacga tcagtttcat ttcgtgcaga cattcgaagt
    12241 aagccatttc aggcgcgtaa ccggcttcgg tcagggtttc aaaacccgcc ttgatcaact
    12301 cgaccacgcc gccgcacaat acggcttgtt cgccgaacag atcggtttcg gtttcttcgc
    12361 ggaaagtggt ttcaatcaca ccgcctttgg tgccgccgtt ggcagccgca taagacaggg
    12421 cgatgtcttt ggctttgccg gaattgtctt ggtaaacagc aatcagagaa ggcacgccgc
    12481 cgccgcgttt gtattcactg cgtacggtat gacccgggcc tttgggggca accataatca
    12541 cgtccaagtc ggcacgcgga acgatttggt tgtagtgcac gttgaagccg tgtgcaaatg
    12601 ccagcgttgc gccttctttc agattggctg caacttcggc gtgatagacg gcaggcatgg
    12661 tttcgtcagg cagcagcagc ataacgacat cggcttcttt ggtcgcttca gcaacggttt
    12721 tgacgacatg gccggctgct tcggcttttt tccaagaaga accttggcgc agaccgatca
    12781 ccacgtttac acccgaatct ttcaggttgg cagcatgggc atgaccttgc gaaccgtaac
    12841 cgatgatggc aacggttttg cctttgatca gggacagatc ggcatcttta tcgtaataga
    12901 cttgcatttg atttccttta aggtaaatgg ttgtcgaagc cttaaaatgt tgagcggctt
    12961 cggacgggtt aaacagagtg tgccgcttaa gcggcaactt cattcatcaa tacgatttcc
    13021 aacgcttcgg ttttgccgtc gacggactgg acgaaggctt ggaaatgcgc gctggcgtta
    13081 tgttcgtcaa tagctgcttg agatttccaa ttttccacga aaacaaaacg gttcggtttg
    13141 ccgatttcct gatggagatc gtagctgatg ttgccctctt ctgcacggct ggctttgacc
    13201 agttctttaa actgtgctac cagtgtttcc gtgtattccg gtttgacggt aaccagtgcg
    13261 acaattttaa tgttcgacat aaatctctcc tgccgttcgt ttttcagacg gcattcaaat
    13321 accgtgccgt ctgaaaggtt acggcgttaa attttcaaaa tacgctcacc gcgaccgatg
    13381 ccggccgcgc ctgtgcgtac ggtttccaaa atttgggcgc gtccgaccgt ttccaaaaag
    13441 gaatccagct tgtcggtcga gccggtaatt tcaatcgtat agctgcggtc ggttacgtcg
    13501 atgatgctgc cccggtagat ttcggtcaag cgtaaaaatt cgtcgcggtc tttgccggcg
    13561 gcacggactt ttaccaacat cagttcgcgt tcgacaaaac ggctttcatt caaatcgacc
    13621 actttaatca cttcaatcaa tttattgagt tgcttggtga tttgttcgat gacctgctcg
    13681 tctccgtggg taacgatggt catacgggac agggttttgt cttcggtcgg cgcaaccgcc
    13741 aaagaatcga tattgtaatc gcgtgcagag aacaaaccga ccacgcggct catcgcacct
    13801 gattcgtttt caatcagaac agataagata tgtcgcattt gcctctcctt acgccttttc
    13861 gtccgcacgc atatgcggcg gaagtaccat ttcgtccaaa cctttgccgt tgccgaccat
    13921 gggcatcaca ttctgtttct ggtcggtcag gaagtcgata aacaccagcc tgtctttttg
    13981 gttcaatgct tccaacaacg caccttccac atcagacttc ttgtccacgc ggataccgat
    14041 atggccgtat gcctcggcaa gtttgacgaa atcgggcaaa gaatcgaaat aggtttccga
    14101 ctctcgaccg ccgtaatata tttcctgcca ctggcgtacc ataccaagat aaccgttgtt
    14161 cagcgtaatg acgttaaccg gaatccgata ttggaaacag gtggacagct cttggatgtt
    14221 catctgaatc gagccgtcgc cggtaataca gaatacgtct tgatccgggg cggcaagttt
    14281 tgcaccaatc gcataaggca gacccacgcc catcgtaccc aaaccgccgg aattgagcca
    14341 ttggcgcgga cgttcgaagg gataatattg agccgcaaac atttgatgct gccctacatc
    14401 cgatgtgatg attgccgaat tgccggtaat ctcggcaagc ttctgaatca catattgtgg
    14461 cttgataatt tcgctgccgt tgtcaaacca caagcaatcg cgggaacgcc attcctctat
    14521 ggttttccac catttgccca aagcatcttc agacggcacg gactcttgtt tttgccacag
    14581 cgcaaccatc tcggacaaaa cgtttttcac gtcgccgaca atcggaatgt ccgccttcac
    14641 gcgtttggcg atgctggaag gatcgacatc gatatggata accttcttcg ccttctcgaa
    14701 aaatttggac ggtacggaaa ccacacggtc gtcaaaacgc gcacctacgg caagaacgac
    14761 atccgcattc tgcatggcaa ggtttgcctc gtaagtaccg tgcataccga gcataccgag
    14821 gaattggcgg tcgccggaag gataagcgcc caagcccatc agcgtacccg tgcacggagc
    14881 acccgtcatt cggacaaatc gggtcagctc ttcagaagca ttacccaaca ccacgccgcc
    14941 gccaaaatag acgaccggac gtttggcaga tgccaacatc tgcacggcct ttttaatctg
    15001 accgatatgc ccttgaacaa ccggttgata cgaacggata aaaatgtctt cctgaggata
    15061 gctgaatttc gccatcgcct gcgtaacatc tttcgggaca tcaaccacca cgggccccgg
    15121 tcggccgctt gcggcaattt ggaacgcctt tttaatggtt tccgccaact cattgatgtc
    15181 cgtaaccaga aaattgtgtt tgacgcacgg acgggtaata cccaccgtat caacttcttg
    15241 gaacgcatcc gtaccaatca gggaattgcc tacctgcccg ctgatgacca ccatcggaat
    15301 cgaatccgta taggcagtag caataccggt cagtgcattg gtaacgcccg ggccggatgt
    15361 aaccaatgcc acgcccacct taccgctgac gcgcgcatac gcatctgccg cgtgtactgc
    15421 cgcctgctcg tgacgggtca gaatgtgctt gaatttattg agttggaaaa gggcatcgta
    15481 gatttcgata accgcaccgc cgggataacc gaaaacgtac tcgacacctt cggctttgag
    15541 actctgcact atgatttgcg cgcctgatag ctgcataacg acctctttta tacggtttca
    15601 aaccaatagg gacaaaccgc tttgccacag cacctgtaat gcaattccac caagcagcga
    15661 tttagggtac gcgcattggg ggaacacggc aacagacgga ttatccaatc aattggaaag
    15721 gaacacagag tttgtgaaaa agagtagaaa cgataacgca aaccgacagt tcaatcaaga
    15781 aaaatctttc atcttttaat atttttcgaa agcagagaaa ttattgattg attttaaaag
    15841 aataaaatca ggagtacctt ttttgaaaga tggaaattgt tgacagtttg tgtaggaggg
    15901 gcagatgtga aaaacccttc ttcgatatca agaattgtaa aatttacagg gtttcatccc
    15961 aataaagact cgggatattg attgaacttg attttatttt tgatatatca aaaatattcc
    16021 caaccatact tcctgaaaat ggctcattgc accggactgt attggacggc attgacagaa
    16081 ccaagagggc taacaacgac ttaatatatt gattgtatag tggattaaca aaaatcagga
    16141 caaggcgacg aagccgcaga cagtacaaat agtacggcaa ggcgaggcaa cgccgtactg
    16201 gtttaaattt aatccactat atttagtctt atctatttca ttaaacagca atagacaaaa
    16261 aaataaccgc tctaaaagcg gttgtggtgc ccagggtcgg actcgaaccg acacaccttg
    16321 cggcggggga ttttgagtcc cctgcgtcta ccaatttcgc cacctgggct ggtgaagaag
    16381 tcgtcattat aatggctttt gaaattctgt aaaccttttt ttgaaattat tttatctgtt
    16441 tttattttat ttttgatttt aaatagaatt tttattattt taatcttact gttctttccg
    16501 ctccaaagat tctgtatgat tcggcaattc ctgccgtgca gacaacgtaa aaaaatacta
    16561 cattaaatct gccaaacgcg ttaagatgga aatattcaaa ttccgtacga atcaggtttt
    16621 gctatttatt cttgggagat tgtcatgttt tccgtaccgc gttccttttt gccgggcgtt
    16681 ttcgtacttg ccgcgcttgc cgcctgcaaa cctcaagaca acagtgcggc gcaagccgct
    16741 tcttcaagtg catccgcgtc agctgcggaa aatgcggcaa agccgcaaac gcgcggtacg
    16801 gatatgcgta aggaagacat cggcggcgat ttcacgctga ccgacggcga aggcaagcct
    16861 ttcagcctga gcgatttgaa aggcaaggtc gtgattctgt ctttcggctt tacgcactgt
    16921 cccgatgtct gcccgacaga gcttttgacg tacagcgaca cgttgaagca gttgggcggg
    16981 caggctaagg acgtgaaagt ggtgttcgtc agcatcgatc cggaacgcga cacgcctgaa
    17041 atcatcggca agtatgccaa acagttcaat ccggacttta tcggtctgac ggcaacgggc
    17101 gaccaaaacc tgccggtcat caagcagcaa taccgcgtgg tttctgccaa agtcaatcaa
    17161 aaagacgaca gcgaaaacta tttggtcgac cactcttccg gtgcgtatct catcgacaaa
    17221 aacggtgagg ttgccatttt ctcgccttac ggaagcgagc cggaaacgat tgctgccgat
    17281 gtaaggactc tgctctgata aaaccgtatg ccgtctgcac cgtcggcgcc tattcagacg
    17341 gcattattgt ttcaaccgac aaaggacatc cacaccatgc aggataatgc tttgaccatc
    17401 gccttatcca aggggcgcat ttttgaggag acgctgccgc tgcttgccgc tgccggcatt
    17461 gttccgactg aagagcctga aaaatcgcgc aagctgatta tcgggacgaa ccatgaaaac
    17521 atccgccttg tcattgtccg cgcaaccgat gtgccgactt atgtccgcta cggcgcggcg
    17581 gacttcggca ttgcgggcaa agacgtgctg atcgaacacg gcggcacggg gctttaccgg
    17641 cctttggatt tggagattgc caagtgccgc atgatggttg ctgtgcgtaa agggtttgat
    17701 tacgaagcag cttcgcaacc cggatgccgt ctgaagattg ccacgaagta tcctgaaatc
    17761 gcggcatctc attttgccgg caagggtgtc catgtggaca ttatcaaact gtacggctcg
    17821 atggaacttg cgccgctggt cggcttgagc gatgcgattg tggacttggt ttcgacgggc
    17881 aacaccttga aggcaaacgg cttggaagca gtcgaacaca tcgtcgacat ttccagccgc
    17941 ctggtggtca acaaggctgc tttgaaaacg aaatacgcgc tgctggagcc gattattcag
    18001 gcgttcggcg gcgcagtgaa ggcgaagtaa gcatccattt gaataaagat gcgttttcag
    18061 acgaccctat ccgttcccgc cgacaggtcg tctgaaaata tcaccggcag taaactgtat
    18121 aggagaagtt aaaatggttg caaaaataaa aaaattctca gattcaaccc tttccgtttt
    18181 gaataacggc gagcgtcggt tttatgtcta ttgtctgacc gacctgaaaa aagacaaaat
    18241 cctctacatc ggcaaaggct gcggtaatcg tatcttcgag catgaatggg ttgctagtcg
    18301 ttcacaagac ccagtctccg gcgagattat cgatcggaaa ctcaaagcca tctccaaatg
    18361 caagaaactc ggtcgctata tcatcagcta tcatctgact gaagtcgaag cactcgccgc
    18421 cgaatctgcc ttaattcatt ttgttaaatc tgtcttgggt aaaaaactca aaaataaaat
    18481 tgccgggcat ggtccgggtg gtattagcgt agaagaacta gatcgccgct ttggattctc
    18541 ttctctccca cttaacgaga ttaaccccga cgggctgatt ctcgccatca aaatccacaa
    18601 tgctttcgat ttagatactg acgaagaatt agactacctt ttcgacaacc aagacgatgc
    18661 caacctcaaa tcgcgtacgt tgggcaactg ggttatcggt aaagatgttg cttcaaaagt
    18721 gaaatacgtt atcggcgttc acaccggtct gcaaaacgct gttgtcagtg catacgaagt
    18781 ggacggtttt gaaacaatgg ttgaggaaac caaaaacggt agaaaacaat cccgttaccg
    18841 tttccgcact acctctcgta gcgaagaggt attagccaaa ctcggtctgc aacaaaaatg
    18901 cctgcccgaa ttgaagtttg gtagcggggg agaaaaagcg tatatcagac ccaaaacaga
    18961 gacagaaact gaacaagaga atattcagac gacccccaat ccaaaaataa aaaaggaaaa
    19021 aaccaaatca tgaaaaaact caacacccaa tcgcccgatt tccaagccgg actcaaagcc
    19081 ctgctggctt ttgaaaccgc gcaaaacccc gaaaccgaac gcatcgtcgc cgacatttgc
    19141 gccgacgtgc aaaagcgcgg cgatgcggct ttgattgaat acaccaacaa attcgatcag
    19201 acaaacgcta aaagcatcga tgatttaata cttacgcaag ccgatttgaa cgcggcgttc
    19261 gagcgcattc cgaacgacgt tcagacggca ttgcagaccg ccgccctccg tgtcgaaagc
    19321 taccaccaac gccaaaaaat ggaatcgtgg agctacaccg atgaagacgg cacgctgttg
    19381 ggacaacaga ttaccccgct tgaccgcgtc ggcatttacg tccccggcgg caaggcggca
    19441 tatccgagtt ccgtcatcat gaacgccatg cccgcccacg tcgcaggcgt gaaagaaatt
    19501 attatggtcg tgccgacacc gaaaggcgaa cgcaacgaca tcgtgcttgc cgccgcatac
    19561 gtcgccggcg taaccaaagt cttcaccgtc ggcggcgcgc aggcggttgc cgccctcgcc
    19621 tacggcacgg aaaccatccc ccaagtcgat aaaatcaccg gtccgggcaa cgccttcgtc
    19681 gccgccgcca aacgccgcgt gttcggcgtg gtcggcatcg acatggtggc ggggccgtct
    19741 gaaatcttgg tcatcgccga cggcacgaca cctgccgatt gggtggcgat ggatttgttc
    19801 agccaggccg aacacgacga aattgcccaa gccatcttaa tcggcacgtc gcaagcgtat
    19861 ctcgacgaag tagaagccgc tatggacagg ctcatcgaaa ccatgccgcg ccgcgacatc
    19921 atcgaagcct cgctcggcaa caggggcgcg atgatactcg ccaaagactt ggacgaagcc
    19981 tgcgaaatcg ccaactacat ttcccccgaa cacttggaat tgtcagtcga aaatccgcag
    20041 gaatgggcga aaaaaatccg ccacgccggc gcgattttca tgggacgcta caccggcgaa
    20101 agcctcggcg actactgcgc cggcccaaac catgtgttgc ccaccagccg aaccgcccgc
    20161 ttttcctcgc ctttggggac atatgatttc caaaaacgct ccagcctgat tcaggtttcg
    20221 gaacagggcg cgcaaaaatt aggcgaaacc gccagcgtgc tggcacacgg cgaaagcctg
    20281 accgcccacg cccgcgcggc agagttccgt atgaaataat gccgaaacgg cgtacaggca
    20341 tattccaacc attaaggaaa cacgatgaaa tccgtccgct ccttcatccg cgacgacata
    20401 caagctatgt cggcatatca gattgccgac gttccgcccg gctttgccaa actcgattcg
    20461 atggaaagtc ccgtccaccc ttttgccgga catgaaacgc tgttgcagga atggcaggca
    20521 cggcttgccg ccgcgcccat ccatctttac cccaatccct ccggcagcgg tttacaggaa
    20581 gcattacgtt cggcgttcga cattcccgac tgcgccgaca tcgcgctggg caacggctcg
    20641 gacgagctga tacagttcat cacgatgctg accgccaaac cgggcgcggc aatgttggca
    20701 gccgaaccca gtttcgtcat gtaccgccac aacgccgcgc tgtacggcat ggattatgtc
    20761 ggcgttccac tgaacggaga tttcaccctc aacctgcccg ccgtcctcga agccgtcagg
    20821 aagcaccgcc ctgccctgac ctttatcgcc taccccaaca accccaccgg cgtatgtttc
    20881 acgcgtgccg aaatcgaagc cgtcatcgaa gcttcagacg gcatcgtcgt cgtcgacgaa
    20941 gcctacggcg cattcaacgg cgacagcttc ctgccgcagg cgggcagcat tcccaacctc
    21001 atcgtcatgc gtaccgtcag caaaatcggt tttgccggac tgcgtatcgg ctatgcggca
    21061 ggctgtcccg aagtcatcgg cgaactgcaa aaaatcctgc cgccctacaa tatgaaccaa
    21121 ctgagcctga ccaccgccaa actcgccctg caacactacg gcatcatctc tgccaacatc
    21181 gacagcctga aaaacgaacg cgaacggatg ttcgccgaat tgggcaaaat atgccgtctg
    21241 aacacctttc caagtcaggc aaacttcatt accatacgcg tacccgatgc cgatttgttg
    21301 tttgacacgc tcaaacaaaa ccgcatcttg gttaaaaaac tgcatggcgc gcacccgctt
    21361 ttggaacact gcctgcgcat taccgtaggc agccccgcac aaaacgatgc cgttctcaac
    21421 atcattcgcc aactttactg ccaaccaacg gatttcctat gaatttgact aaaacacaac
    21481 gccaactgca caactttctg accctcgccc aagaagcagg ttcgctgtcc aagctcgcca
    21541 aactctgcgg ctaccgtacc cccgtcgcac tctacaaact caaacaacgc cttgaaaagc
    21601 aggcagaaga cccagatgca cgcggcatcc gtcccagcct gatggcaaaa ctcgaaaaac
    21661 acaccggcaa acccaaaggc tggctcgaca gaaaacaccg cgaacgcact gtccccgaaa
    21721 ccgccgcaga aagcaccgga actgccgaaa cccaaattgc cgaaaccgca tctgctgccg
    21781 gctgccgcag cgttaccgtc aaccgcaata cctgcgaaac ccaaatcacc gtctccatca
    21841 acctcgacgg cagcggcaaa agcaggctgg ataccggcgt acccttcctc gaacacatga
    21901 tcgatcaaat cgcccgccac ggcatgattg acatcgacat cagctgcaaa ggcgacctgc
    21961 acatcgacga ccaccacacc gccgaagaca tcggcatcac actcggacaa gcaatccggc
    22021 aggcactcgg cgacaaaaaa ggcatccgcc gttacggaca ttcctacgtc ccgctcgacg
    22081 aagccctcag ccgcgtcgtc atcgaccttt ccggccgccc cggactcgtg tacaacatcg
    22141 aatttacccg cgcactaatc ggacgtttcg atgtcgattt gtttgaagaa tttttccacg
    22201 gcatcgtcaa ccacagtatg atgaccctgc acatcgacaa cctcagcggc aaaaacgccc
    22261 accatcaggc ggaaaccgta ttcaaagcct tcgggcgcgc cctgcgtatg gcagtcgaac
    22321 acgacccgcg catggcagga cagaccccct cgaccaaagg cacgctgacc gcataaaaaa
    22381 ccataccgtc tgaaacaccc gcaggctttt cagacggtat cggaacagat aagattacac
    22441 tacactacaa acagaaaagg agtaaacatc atgtccgcaa acgaatacac acaaatcggc
    22501 tggataggct tagggcaaat gggtctgcct atggtaacgc ggctcttgga cggcggcatc
    22561 gaagtcggcg tatacaaccg ctcgcccgac aaaactgccc ccatctccgc caaaggcgca
    22621 aaagtttacg gcaacaccgc cgaactcgtc cgcgactatc ccgtcatttt cctgatggtt
    22681 tccgactatg ccgccgtgtg cgacatcctg aacggagtcc gcgacggatt ggccggcaaa
    22741 atcatcgtca acatgagcac catctccccg accgaaaacc ttgccgtcaa agaacttgtc
    22801 gaatctgccg gcggacaatt tgccgaagcc ccagtttctg gttcggtcgg tccggccacc
    22861 aatggcacgc tgctcattct gttcggcgga gacgaaactg ttttaaaccc gttgcagaaa
    22921 gtgttttccc ttgtcggcaa aaaaactttc cacttcggcg aagtgggcaa aggctcgggc
    22981 gcgaaactcg tcttaaactc gctcttaggc attttcggcg aagcgtacag cgaagcgatg
    23041 ctgatggcgc ggcagttcgg catcgatacc gacaccatcg tcgaagccat cggcggctcg
    23101 gcaatggact cgcccatgtt ccaaaccaaa aaatccctgt gggcaaaccg cgaattcccg
    23161 cccgccttcg ccctcaaaca cgcctccaaa gacctcaacc tcgccgtcaa agagcttgaa
    23221 caggcaggca acaccctgcc cgccgtcgaa accgttgctg ccagctaccg caaagcagtc
    23281 gaagccggct acggcgaaca ggacgtttcc ggcgtttacc tgaaactggc agaacactga
    23341 cttccctttc caaacaatat gccgtctgaa atattttcag acggcatttt tatcgcccca
    23401 cgcttaaaat cagtcccgat tatgac
//
LOCUS       DAWFIE010000023        22533 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWFIE010000023 DAWFIE010000000
VERSION     DAWFIE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22533
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.023"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(166..642)
                     /gene="greA"
                     /locus_tag="WGQ71_000863"
     CDS             complement(166..642)
                     /gene="greA"
                     /locus_tag="WGQ71_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ0706909.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1034..2335
                     /gene="aroA"
                     /locus_tag="WGQ71_000864"
     CDS             1034..2335
                     /gene="aroA"
                     /locus_tag="WGQ71_000864"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ0706910.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGKEHYPPLHIGKRQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADARYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPKTLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETTE"
     gene            complement(2378..2911)
                     /locus_tag="WGQ71_000865"
     CDS             complement(2378..2911)
                     /locus_tag="WGQ71_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0706911.1"
                     /translation="MFPPDKTLFLCLSALFLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTAIGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGTHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(3119..4645)
                     /locus_tag="WGQ71_000866"
     CDS             complement(3119..4645)
                     /locus_tag="WGQ71_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ0706912.1"
                     /translation="MKTRSLLPLLFLLLCSCAAWLPPLEERTESRHFDTASPVRLDKV
                     LHIRHFPSDSRLSDIYLLDDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIICSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAERLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
     gene            complement(4755..6161)
                     /locus_tag="WGQ71_000867"
     CDS             complement(4755..6161)
                     /locus_tag="WGQ71_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0706913.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIATDL
                     DESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRMYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDVKGNDTALDFKGYLTFSAAACLLLLAA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTTSKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSSLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FNRLHVSDGTNLTRNTPS"
     gene            6445..7224
                     /locus_tag="WGQ71_000868"
     CDS             6445..7224
                     /locus_tag="WGQ71_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ0706914.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            7221..7922
                     /locus_tag="WGQ71_000869"
     CDS             7221..7922
                     /locus_tag="WGQ71_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ0706915.1"
                     /translation="MSARENILAKLKKADALPMEEPGVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNISAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            7919..9373
                     /locus_tag="WGQ71_000870"
     CDS             7919..9373
                     /locus_tag="WGQ71_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ0706916.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(9556..10041)
                     /gene="purE"
                     /locus_tag="WGQ71_000871"
     CDS             complement(9556..10041)
                     /gene="purE"
                     /locus_tag="WGQ71_000871"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ0706917.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(10089..10805)
                     /locus_tag="WGQ71_000872"
     CDS             complement(10089..10805)
                     /locus_tag="WGQ71_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ0706918.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAIFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            complement(10805..11473)
                     /locus_tag="WGQ71_000873"
     CDS             complement(10805..11473)
                     /locus_tag="WGQ71_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ0706919.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVSFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPRYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(11484..11669)
                     /locus_tag="WGQ71_000874"
     CDS             complement(11484..11669)
                     /locus_tag="WGQ71_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ0706920.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            11812..13788
                     /gene="mutL"
                     /locus_tag="WGQ71_000875"
     CDS             11812..13788
                     /gene="mutL"
                     /locus_tag="WGQ71_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ0706921.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELDGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKPVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDSASDYPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMNTYAELYKKTDDIDLELSRLEQARFGNMPSETPIPKT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGKLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAVPAML
                     GKADVVSLAKDVLGELAQVGSSQTIEEHENHILATMSCHGSVRAGRQLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            13883..16003
                     /gene="dnaX"
                     /locus_tag="WGQ71_000876"
     CDS             13883..16003
                     /gene="dnaX"
                     /locus_tag="WGQ71_000876"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ0706922.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPITVLSRCLQFVLRNMTSQQVADHLAHVLDSEKIAYEPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQQLALIQAVPNALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPHPEAETTQTPVQTASAAAMPSEGKTAEPVSNQENNDVPPWEDAPDEAQTAAGTA
                     QTSAKSIQTASEAETSPDNQVSKNKAADNETETSLSEVPSENPIQATPNDEAVETEAF
                     AHEAPAEPFYGYGFPDNDCPPEDGAEIPPPDWEHAAPADAEEENNADEGSNDENNTPS
                     APPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARATADK
                     KRLDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADPA
                     AQKILQAFGAQWQPESLELAANRP"
     misc_feature    15072..15111
                     /gene="dnaX"
                     /locus_tag="WGQ71_000876"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            16083..16418
                     /locus_tag="WGQ71_000877"
     CDS             16083..16418
                     /locus_tag="WGQ71_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ0706923.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARDKAEETANKTMGAFAQGL
                     PPGVGDFFR"
     gene            complement(17253..18299)
                     /gene="recA"
                     /locus_tag="WGQ71_000878"
     CDS             complement(17253..18299)
                     /gene="recA"
                     /locus_tag="WGQ71_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ0706924.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGVEMHIT
                     EGKLDETDGERPEE"
     gene            18553..19317
                     /locus_tag="WGQ71_000879"
     CDS             18553..19317
                     /locus_tag="WGQ71_000879"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ0706925.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAVLSAVLDQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGAFPCSDGYYFELL
                     DALIESRLPDIIDIELFAGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKQLAAKPLVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLENGAD"
     gene            19337..21352
                     /gene="rep"
                     /locus_tag="WGQ71_000880"
     CDS             19337..21352
                     /gene="rep"
                     /locus_tag="WGQ71_000880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ0706926.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNAWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(21420..22478)
                     /gene="dinB"
                     /locus_tag="WGQ71_000881"
     CDS             complement(21420..22478)
                     /gene="dinB"
                     /locus_tag="WGQ71_000881"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ0706927.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT         5395 a         6262 c         6114 g         4762 t
ORIGIN      
        1 gttgtctcgc cttagctcaa agagaacgat tctctaaggt gctgaagcac caagtgaatc
       61 ggtttcgtac tatctgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
      121 actataattt cctgcgtgtg tcgggtgtat cgaaatcaag ccgaatcaaa tatatcggac
      181 ttcgataatg tcgtattcgc gcacgccgcc cggggcttgg acttccgccg tatcgccctc
      241 ttccttgccg attaaggcgc gggcaatcgg tgagccgaca tagattttgc cctgtttgat
      301 gtcggcttcg tcttcgccga caatttgata ggtaacgtgt tcttccgttt ccaaatcttc
      361 cagcgtaacc gtcgtaccga acacgatttt gccttcggcg tggatttcgg tcggattgat
      421 gatgtgggca acggaaagtt tgtgttccag ctcggaaatg cggccctcga taaagccttg
      481 gcgttctttg gcggcttcgt attcggcgtt ttcggacaaa tcgccgtgcg atctggcttc
      541 agcaatcgct tcgatcactt cggggcgtgc cacgcttttg agctgctgca attcctgttt
      601 cagcaattcc gcaccgcgta cggtcagggg gattttttgc atcggtcttt tttctccata
      661 ttccggcaca ccgttttgcg gcagcaagca taccgcgtac cgtcttgttt tgtgcgtccg
      721 gatattaaaa taaaaataca agccgcccgg aaaatcggcg gcttgtctgt cgttgaacag
      781 cggctattct accaaattct atgaaattgg caatcgtgcc gcgccgccgg caaacgcgcc
      841 atgtccgcaa caaaagctga aaatatgccg acaaagaaat tttagaaaca aaaaatttaa
      901 aaataatcaa ttttcggcat aaaaaaccac atttacggac tttaaaaccg aaaatgccaa
      961 acctgagatt tttcatacgg catttgcacc agtataatgc aggctgtttt tatctttata
     1021 tttacgtttt gccatgaccg aatccgtccg cctccccgtc gcccgcctca aaccttccac
     1081 cgtcgccctg cccggctcca aaagcatcag caaccgcacc ctgctgctcg ccgccttgtc
     1141 cgacaatgct tgcgaaatcc attccctgct caaatccgac gatactgacc gtatgctcga
     1201 agcactcgat aaactcggcg ttgaaatcga atatcttgcc gaagaccgtc tgaaagtgca
     1261 cggcacaggc ggacgcttcc ccaaccgcac tgccgatttg tttttaggca acgcgggaac
     1321 ggcgttccgt ccgctgactg ccgcgcttgc cgttttgggc ggcgattatc atctgcacgg
     1381 cgtgcctcgt atgcacgaac gtcctatcgg cgatttggtc gatgcgctgc ggattgccgg
     1441 cgccgatgtc gaatatctcg gcaaggaaca ctatccgccg cttcatatcg gcaaacgtca
     1501 agacaacggc gaacgcgtga ttccgattaa aggcaatgtg tccagccagt ttctgaccgc
     1561 ccttttaatg gcgttgccgc tgaccgggca ggcgtttgaa atccgtatgg tcggcgagtt
     1621 aatttccaag ccctatatcg acattacttt aaaactgatg gcgcaattcg gcgtacaggt
     1681 tatcaatgaa ggctaccgcg tcttcaaaat tcccgccgat gcgcgctacc acgcgcccga
     1741 acacttgcac gtcgaaggcg atgcctccag cgcgtcctac ttcctcgcag ccggtttgat
     1801 tgccgccacg cccgtccgcg ttaccggtat cggcgcaaac agcatacagg gcgatgtcgc
     1861 ctttgcccgc gagctggaaa aaatcggtgc ggacgtggtt tggggcgaaa acttcgtcga
     1921 agtctcgcgc ccgaaagaac gtgccgtcca agcctttgat ttggatgcaa accatatccc
     1981 cgatgccgcc atgaccctcg ccatcgtcgc gcttgctaca gggcaaacct gcacgcttcg
     2041 caacatcggt tcgtggcgcg tcaaagagac cgaccgcatt gccgcaatgg cgaacgagtt
     2101 gcgcaaactc ggggcaaaag tcgtcgaaga agccgaagca attcacatca ccccgcccaa
     2161 aacgctgaca cccgacgccg tcatcgacac gtacgacgac caccgcatgg cgatgtgttt
     2221 ctcgctggtt tcgctgttgg gcgtacccgt cgtcatcaac gatccgaaat gcacccataa
     2281 aaccttcccg acttatttcg acgtgttctc ttcactgacc gaaacaacgg aataaggcgg
     2341 cattttgccg cgattccggc gcggcgcggc gggcggctca ttctgtaaaa aaagtatgtg
     2401 cgccgaggta gtttttggcg taaaacggtg tggagagttt ttcggttttg atggttttgc
     2461 cgctgctggg ggcatggatg aattcgccgt tgccgatata gagtccgacg tgtgagtagc
     2521 ggtgtgtgcc gccggtgttg aagaatacga ggtcgccggc cttaaggcgg ctgtcgggga
     2581 ttttgcggct tgccgccgcc atgtcgcggg cggtgcgcgg cagcttgacg ttgagggcgt
     2641 ttttgtaaac gaattgaatc atgccgctgc aatcgaagcc gattgcggtg ctgctgccgc
     2701 cccatttgta gggcgtgccg atgagtccga ggctgtggag catgagttcc tgcgagcctt
     2761 gtgtgcggtc gatgtggctg atgcggacgg cttggatttg ccggactgtc tgtttgggtt
     2821 tcggttggcg gtgtttgccg gaggtcgtgc cgcatgaggc gaggaacagt gcgctgagac
     2881 agaggaaaag ggttttgtcg gggggaaaca tggtttttcc tttgcgggtt cggatatccg
     2941 tctgaaggtg tttcagacgg tatagtggat taataaaaat caggacaagg cgacgaagcc
     3001 gcagacagta caaacagtac ggaaccgatt cacttggtgc ttcagcacct tagagaatcg
     3061 ttctctttga gctaaggcga ggcaacgtcg tactggtttt tgttaatccg ctatatttct
     3121 ataataaact ttctatgggc agcagggata ggatttttgc ggcgatgcgt ttccaaagtt
     3181 tggcttcggg ttcgttcggg taggtttttc gggtggcggg atcatgccat tgcaggcggt
     3241 tgtgcctgtc gagggtaacg cggtaggcgt attcgggtgt ggtatcggca agggtgcgct
     3301 ccatctgttc tgcgattttg gggctttcga taacaacgcc catttcggtg ttgagacgcg
     3361 cggaacgggg gtcgaggttg aacgaaccga tgaagatgcg tttgccgtcc acaatgaagg
     3421 ttttggcgtg caggctggtt acggagctgc cggtcaggcc tttgtctttt gtggcgggga
     3481 cggcatggtt gggttgcagc tcgtagagtt tgatgccggc tttgagcagc ggttttcggt
     3541 atttgacata gccggaatgg acggcggcaa cgtcggtcgc ctgtagcgag ttggtcagaa
     3601 cggtaacgtc tatgccgtcc tgcaccagtt ttgccagtgc gtctgtgccg gattttgtgg
     3661 ggacgaaata gggtgaaacc agatagacgc ttttttcggg ctgtttgagc gcgtcttgca
     3721 gccgctcggc aatcggcggt ttgcggcggt cgcggtcgag tccttttgca gggtcgtcgc
     3781 tgatgaggcg ggtttggacg ctctgccagt cgatgcgtcc tgcctgtatt ttttggtaga
     3841 ggggcgactg ttcgacggtt tcgcggtagc gcaggagcgc gtgtctggac gtttcgtcgt
     3901 tgtatccgag tgcttgaaga cccttgccga tgttgccgct gcagatgatg cgcgtggcgt
     3961 tgtgggcgga atggcttgcc cagtagcggt cgaagtcgtg cgatacttcg ccgacgacgc
     4021 tgccggtggc gaggatgtcc aggtcggcga aaacggtgtc ctcaccgact ttgaagtatt
     4081 cgtcgccgat attgcgtccg ccgagtatgg tggcgcggtt gtcggcggta aaggatttgt
     4141 tgtgcatgcg gcggttgagg cgggggaagt cggtcaggta gccgagtgca cgccattttc
     4201 gtaagacgaa ggggttgaac aggcgcactt cgatattggg atggctgtcg agggcgagca
     4261 ggaggtcgtc caatccgcgc gtgttgttgt cgtccaacag caggcgtacg cgcacaccgc
     4321 gttctgcggc aaggtacacg aggttgaaca gcagtcggcc ggaaatgtcg ttgcgccaga
     4381 tgtagtattg caaatcgagg ctgtgttcgg cagattcgat aagggcggcg cgggcggcaa
     4441 aggcttcgtg ggggtcgtcg agcaggtaaa tgtcggacag gcggctgtcg gacgggaaat
     4501 ggcggatgtg caggacttta tccaaacgga cgggggaagc agtgtcaaaa tggcggcttt
     4561 ccgtgcgttc ctccaagggc ggcagccatg cggcgcacga gcagagcagg aggaacaaca
     4621 ggggaaggag gctgcgtgtt ttcataggaa atcggttcag acggcattgc ctgtgttttg
     4681 gggttggtgc gcatggaagt gcggcatcat aatccaaacg ttgaaacggg taaaagtttt
     4741 gcgtgcggac cgcttcagga cggtgtattc cgtgtcaggt tggtgccgtc tgaaacgtgc
     4801 agccgattga ataccagcga tgaggcaagg gtgatgccgc cgatgctgag cagggtcata
     4861 cggaaggcgg aatgcagacc tgaagaagcc ggtatcagaa atgtccagtt tttaaggatt
     4921 aatgcgccga caacaatgcc catgctgatg gcaagctgtt ggttgaccgc catcaggctg
     4981 ttgccgctgc cggtctgttg cgggcgcaaa tcggcgaggg tcagtgtatt catggcagaa
     5041 aactgcaggg agttgcacgc gccgatcgcc agcgagagga aaatccaaat ccacagcgac
     5101 gagtttccgt caggcagggc gagcagcatg atgaaggcgg caagcagctt ggtgttccaa
     5161 agcagtaccg tgcggtagcc gaaacgtttc atgagcggtg caatcagcgg tttgaccagc
     5221 agcgaagaca gggcgacggg ggcgaccagc caacccgaca ggcttgcgcc gaagccgaaa
     5281 gcgacttgaa acatcagggg catcagaaaa ggaatcgagc tgatgccgag acggctgaac
     5341 agattgcctg ccagtcccag acggaaagtg cgtatcagaa acaggtcggc ggaataaatc
     5401 ggtttggacg tggttttcat atgtcggaaa taacggcgtg caaacagcag tccgccgcac
     5461 agcggcaaca gtgcaaaata cggaggcagc gcgtgcgaca ggctttctgc agcaagcagt
     5521 agcaggcagg cggcagcgga aaaggtcaga tagcctttaa aatccagagc ggtatcgttg
     5581 cctttgacat cgggcatgat gttgcgtccc aatataaacc ccagcagacc gatgggcagg
     5641 ttgagcagga aaatccagtg ccacgaagcg tattcgacca gataaccgcc cgccaaaggc
     5701 cctaaaaccg gcccgattaa tgcgggcata accgcataat tgatggcgtt gagcagcttg
     5761 gacttgtcgt acatacgcaa gatggtcaga cgcggtatcg gaaccagcat cgaaccgccg
     5821 atgccctgaa cgacacggga aagcgtcaat tcaaacagcg aacccgatgc ggcgcacaat
     5881 gccgatccga gcataaaaac ggcaatcgaa ccgaaaaaga cttttttcgt tccgaacctg
     5941 tccgccaaat aaccgctcaa aggaatcagc agggcaaccg tcagcgtata ggcaacgact
     6001 gccagttgca tatccagagg cgattcgtcc aggtcggtgg caatttcagg cagtgcggta
     6061 tttaaaatgg tcgcatccaa catctgcata aaaatggcaa ttgccagcag aagcggcagc
     6121 caaggggatg gtgcgcgggc ggatagggtg tttttttcca tagggcgatt gtaccccatc
     6181 cttgtgccgt tattgttttc agacgctgtc tgaatgccgt cagagtcggc atcttgaatg
     6241 ttcacaagca aacgaaccgg tattgcattg taatgataat tattatcgaa aaacatcaga
     6301 ttaaggtaca gtaagcgtta tggggtagtt tgtaagaaaa accggattat tttttaaaat
     6361 tagacttgac ccgcaacagt caattactta aagtaaacgc ttacctttct acagaaaaaa
     6421 acgggtttcc cgttatcaaa aaacatgagc gcaaccattc ccccaaaaat catccgatac
     6481 gacagcaatc cgaccgatgt ctattttttc ggcacttgcg tccttgatct ttttatgccc
     6541 gaagcaggca tggatgccat taccctaatc gagcaacagg gcatacgcgt ccatttcccg
     6601 atggcgcaaa gctgctgcgg ccagcctgcc tattcatccg gccatccgac cgaagccttc
     6661 gatgtcgcca aagcacaact cgaccttttc cctgaaaact ggccgatcgt cgtgccgtcc
     6721 ggctcgtgcg gcggcatgat gaaacaccac tggccgacgc tgtttaaagg cagcgagtac
     6781 gaggaaaggg ctgtggattg cgccggccgc atcatcgagt ttacccattt cctgcttgcc
     6841 atcggtttca aacccgaaga caagggcgaa ccgctcaaag tcgccgttca cacttcctgc
     6901 gccgcccgcc gcgaaatgaa tgtccatctt tcaggctggc aactgattga cggtatggaa
     6961 aacgtcgaac gcatcgtcca cgaccacgaa agcgaatgtt gcggcttcgg cggcacattc
     7021 tccgtcaaac aagccgatat ttccggcgca atggtaacag acaaagtcgc cgcgctgaaa
     7081 gaaaccggcg caaccgaaat catcagcgcg gactgcggct gtatgatgaa catcggcggc
     7141 aaaatcgcca aggacgagcc ggatatgccg cgtccgaaac atatcgcatc cttcttgttg
     7201 gaacgcaccg gaggcaaagc atgagcgcgc gtgaaaatat tttggcaaaa ctgaaaaaag
     7261 ccgatgcctt gccgatggaa gagcctgggg ttttcgatta ttaccgcgaa aagggcgttt
     7321 cttgggacag cgaagccgag cgtctgaaac attgggctgc cgcaatgcgc gcggtcaaaa
     7381 ccgaaattta ttgggtgacg aaaagcaatt ggatgcaggt tttccgcgaa gcggcagaag
     7441 gcaagggttt gaaaaacatc ctgctgccct tggcgaccga acacggacaa attgcccgtg
     7501 ccgcattggc ggacagcaat atcgaaccga ttgccttcga gcgcgaaatc gatacttgga
     7561 aaaccgagtt tttcacgaac atcagtgcgg gcttcagcgg cgcgcaatgc ggcatcgccc
     7621 gcaccggcac gctgatgctg ttttccagcc ccgaagaacc gcgtactttg agtctcgttc
     7681 cgcccgtgca tttctgcctg ttcgatacgt ccaagatgta caacgagttt cataatgccg
     7741 tcgaaggcga aaaactggtg gaaaacggta tgccgaccaa tgtattcctg atttccggcc
     7801 cgtccaaaac cgcagacatc caactgacgc ttgcttacgg cgcgcacggc ccgcgcgatt
     7861 tggtcatcct cgccatcctg cccgaccaca tttcccctgc cgatttggag gaaaacgcat
     7921 gactacgcaa accatcaagt tccacatgaa gccggaaact ttcaagcaaa acgccgcaat
     7981 ttcccttcaa gacaagcctt tgcgcaaaag cctgcgtacc gcgatggata tgctgatgac
     8041 caaacgcaaa gccgttttga ccgacgaaga agagctgcaa agcctgcgcg atttgtgcga
     8101 acacatccgc cagcgttccc tgtccaaatt gcccgcgctg ttggagcagt tggaagaaaa
     8161 cctgaccaag ttgggcgtga aagtacactg ggcggaaacc cctgccgaag cctgccaaat
     8221 tatccacgac atcatcacag ccaaaaacgg caagctgatg gtcaaaggta aatccatggt
     8281 cagcgaggaa atcgagctga accattatct tgaagcaaaa ggcattaaag cggtagaaag
     8341 cgacttgggc gagttcatcg tccaaatggc aggcgaaaaa ccgacccata ttgtgatgcc
     8401 tgctatccac aaaaccaaag aacaggttag cgaactgttc caccaaaacc tcggtacgcc
     8461 gctgacagac gatgtagacc aactgaccgg cttcgcccgt aaagcactgc gcgatattta
     8521 cagcactgcc gatgtcggtt tgagtggcgt aaacttcgcc gttgctgaaa caggtacgct
     8581 gtgtctggtg gaaaacgaag gcaacggtcg cttgagtacc accgtaccgc ccgtgcatat
     8641 cgctattacc ggcattgaaa aagtggtggc gaaattgtcc gacatcccac ccttgtacag
     8701 cctgctgccg cgttctgcca ttggtcagaa cattaccact tatttcaata tgattaccgg
     8761 cccgcgccgt agcgaagagc tggacggtcc tcaagaaatg cacttggtcc tgcttgacaa
     8821 cggtcgcagc caagcctatg ccgaagacca aatgcgccgc accttgcaat gtatccgttg
     8881 cggcgcgtgt atgaaccatt gcccggttta tacccgtatc ggtggcgcgg catatggcac
     8941 aacctatccc ggtccgattg gcgagattat ttccccgcac ctgttaggct tggatgccac
     9001 ccgcgacctg ccgaccgcct gtaccatgtg cggtgcgtgt gtggaagtct gtccggtacg
     9061 catcccgatt accgaacaaa tgcagcgttt gcgcgttgaa gcgcaacgtt cgccgaccga
     9121 aaccgtgccg caccccatcc gagggcaagg cgcatcgcat accttcggcg aacaaatggc
     9181 gtggcgcaca ttcaacggta ttttcagcgg cagcaaaacc taccgcgcct tcggttgggc
     9241 agccaccaag ttccgcaacc tgaccccgcg caaacagttg ggttggacgc aaaaccgcgt
     9301 gccgatgaaa ccggcgaaga aaaccctgca cgaactaatg gcagaaaaaa tgcgccaaaa
     9361 agaacaggca taaaaagttg ttcgcaaaaa tgccgtctga aacccgaaac agggcttcag
     9421 acggcatttg tatagtggat taacaaaaat caggacaagg cgacgaaacc gaagacagta
     9481 caaatagtac ggcaaggcga ggtaacgccg tactggttta aatttaatcc actatatatt
     9541 cgcggacggc gggttttaaa tctgttccaa ttccatattc aaaacagtct gttcttgttt
     9601 ggctcggaag tctgccagtt tttgcgccag ttcggggttt tcgttggcga gcatggaaac
     9661 ggcgaacaat gcggcatttg ccgcacctgc ctcgccgatg gcgaatgtgg cgacgggtac
     9721 gcctttgggc atttgtacaa tcgataaaag cgaatcttcg ccgcgcaggt atttgctggg
     9781 gacgggtacg cccaaaacgg ggacggtggt cttggcggca accataccgg gtaaatgcgc
     9841 cgcgccgccc gcacccgcga tgatggcttt gatgccgcgc gcccgtgcgg tttcggcgta
     9901 ttggaacatc aaatccgggg tgcggtgtgc ggaaacaacg cgcgcctcat attctacgcc
     9961 gaattcttca agaaactgcg ctgcctgccg cataacgggc caatcgctgt tgctgcccat
    10021 gatgatgccg atttgtatca taaatcctcc ttggtgcgga tggggtaaaa agcggaaaaa
    10081 tggaaaaact atcgtttgcg cacggctgcg gcggcgcgtt ttgccgccgg gctgctcggg
    10141 taagcctgta tcaggctgcg ccaagtcgcc cttgcaatgt ctttctgctg caacctgtat
    10201 tggcattcgc cgattttgaa tatggcttcg ggcgcggttg ggctgtcttt gaaacggttg
    10261 gcgtaacgcc ctccgatttc gatgacggat tcgcagttgc ccatacgcgc cctgctttgc
    10321 agcaacagat acatactgcg ttgcgcgatg ctgccgccgt cgcctccgtc cgcgcctttc
    10381 aacagggagg cagcggcaga aaacctgccg cttttatagt gtttgagtgc ctgattgtag
    10441 aggttttgtg cggtttcgac ggtatgtgcg gatgcgctgc cgccttcggt attgaggtaa
    10501 tgctctttca acttgcggtc gtcgagtttt tggacgtatg ccctgctgga agggtgtgtt
    10561 ttcgcatgct ccagtgcttt gactttgccg tttaaggttt ccacttcgtt cgacaggcgg
    10621 acgagtgtgc cttccagata atccaaacgg tcttgcagag tgggaacggg atagggaata
    10681 gcgtccgaac tacctgcctc tgcctgcggt tcgggccgca tatcctgaat attgcgggaa
    10741 acaggggaag aacaggcggc ggatacggac agccaaatga taaaaagcgg taatttggtc
    10801 ttcattattt tttcagaagc agggtcaagc cgtcgccgac gggcagggtg atggggacaa
    10861 tccgcgtatc gttcggcagg ttttgattga aatctttgag gatgccgacg ctgggcggcg
    10921 catcggaagc cgcttcgcgc atcacccttc cgttcagcaa aatattgtcg atggcgatga
    10981 tgccgccttg acggacgagt ttgaggcaac gctcgaaata tcgcggcgtg ggcggtttgt
    11041 ctgcgtctat cagtgccaaa tcgtagcttc cggcttcacc ctgtgcaatc aaatcatcca
    11101 atgtcagcaa tgcgggttgc aggtgcaggc tgattttatg tgccacaccg gcctcgttcc
    11161 aaacctgacg cgccgtatcg gtaaagctta cattgatgtc gcaggcggta atccgcccgt
    11221 gttcgggcaa cgccaatgca agcgcggtgc tgctgtatcc ggtaaatacg ccgatttcca
    11281 gatatttttc cgcacggatc agctttgcca gccaaaccaa aaccgccgcc tgttcgcgcg
    11341 caatcgccat tttgcccata cggtgatgcc cggtcttctc acgcagccgc gtcaaaacgg
    11401 gatgttcagg ttcgccgatg gcgttcaaat agttttgcag gtccggtgca acattggaca
    11461 gatgggtcgt catttcggcg ggttcagtct tggtaatagg tataaggttt tttcgctact
    11521 tttgccgcct cgaagttttc ctgttcttcg ggattgagtt cgacatccca caaaagcccc
    11581 cggttttcca aacgctgctg ttccaactca ggtttttctt caatcaggcg gttgaggaat
    11641 tgtgtggctt cggattggta gtgatacatc tttgcgctcc aattttacgg gatacggcgt
    11701 gattatactg gtattttcca aacgggataa acggctttta tcaagaatac aggcagaaag
    11761 ataaggggtt ttattataga ataaagcgtt tttgcaacgg aagcctacct tatgccacgc
    11821 attgccgccc tgcccgacca tctcgtcaac caaatcgccg ccggcgaagt agtcgaacgc
    11881 cctgccaacg ccttgaaaga aatcgttgaa aacagtatcg atgcaggcgc aacggcgatt
    11941 gatgtcgagc tggacggcgg cggcatccgc ctgattcgcg tcagcgacaa cggcagcggt
    12001 atccatccgg acgacatcga actcgcgctc caccgccacg ccaccagcaa aatcaaaacc
    12061 ttaaacgatt tggaacacgt tgccagcatg ggctttcgcg gcgaaggctt ggcaagcatc
    12121 gcctccgtca gccgcctgac cctgaccagc cgtcaagaag acagttcgca cgcaacccaa
    12181 gtcaaagccg aagacggcaa actcagcagc cccaccgccg ccgcccaccc cgtcggcacc
    12241 accatcgaag ctgccgaact cttcttcaac acccccgcgc ggcgcaagtt tctcaagtcc
    12301 gaaaacaccg aatacgccca ctgcgccacc atgctcgaac gcctcgcgct ggcgcatccg
    12361 cacatcgcct tctcactcaa acgcgacggc aaaccagtgt tcaaacttcc tgcccaaagc
    12421 ctgcatgaac ggattgccgc cattgtcggc gatgactttc agacggcatc cctggaaatc
    12481 gacagcggca acggcgcatt gcggctctat ggcgcgattg ccaagccaac ctttgccaaa
    12541 ggcaaaaccg acaaacaata ctgcttcgtc aaccatcgtt ttgtccgtga caaagtcatg
    12601 ctccatgccg tcaagcaggc ataccgcgac gtattgcaca acgcgctgac gcctgctttc
    12661 gtcctctttc tcgacctgcc gcccgaagcc gtggatgtca acgtccatcc gaccaaaacc
    12721 gaaatccgct tccgcgacag ccggcaggtg caccaactcg tgttccacac gctcaacaaa
    12781 gcccttgccg acacgcgcgc cgacttgacc gaaagcgtca gcaacgcagg cgaagtgttg
    12841 catgacatta ccggcgttac gcctgcccca atgccgtctg aaaacgacag cgaaaatctg
    12901 tttgatagcg catccgacta cccgacaggc aacaaacccg atacacgcaa tgcctttggt
    12961 tcatcaggca aaaccgcgcc catgccttac caagccgcgc gtgcgccgca acaacacagc
    13021 ctgtccctgc gcgaaagccg cgcggcaatg aatacttacg ccgagcttta caaaaaaacc
    13081 gacgacatcg accttgagtt aagccgatta gagcaggcgc gtttcggcaa tatgccgtct
    13141 gaaacgccta tcccaaaaac agatacgccg ctttcagacg gcatcccgtc ccaatccgaa
    13201 ctgccgccgc tcggcttcgc cattgcccaa ttattgggca tctacatcct cgcccaagcc
    13261 gaagacagcc tgctgctcat cgatatgcac gctgccgccg aacgcgtcaa ctacgagaaa
    13321 atgaaacgcc aacggcagga aaacggcaag ctgcaaagcc aacgcctgct tattcccgta
    13381 acctttgccg cgtcccacga agaatgcgcc gcccttgccg attatgccga aacgctggca
    13441 ggcttcggct tggaattgtc cgatatgggc ggcaacaccc tcgccgtccg cgcagttccc
    13501 gccatgctcg gtaaagccga cgttgtttcg ctggccaaag atgtattggg cgaactcgcc
    13561 caagtcggca gcagccaaac catcgaagaa cacgaaaacc acatcctcgc caccatgtcc
    13621 tgccacggct ccgtccgcgc cggccgccaa ctgaccttgc ctgaaatgaa tgcgctcctg
    13681 cgcgatatgg aaaacacgcc gcgcagcaac cagtgcaacc acggcaggcc gacttgggtc
    13741 aaactgactt tgaaagaatt ggacgcactg ttcttgcgcg gacagtaagc cgaaagtgct
    13801 agaatacgcc gcccgaagcc gccgttcaga cggcattccg acgcaccgac agaaacatca
    13861 cgaccgaaac caagagaaaa atatggccta tcaagttctc gcccgaaaat ggcggcccaa
    13921 aacctttgcc gacttagtcg gtcaggaaca cgtcgtcaaa gccctgcaaa atgccttgga
    13981 cgaaggcagg ctgcaccacg cctacctgct gaccggtacg cgcggcgtgg gcaaaaccac
    14041 catcgcccgc atccttgcca aaagcctcaa ctgcgaaaac gcgcaacacg gcgaaccttg
    14101 cggcgtgtgc caaagctgca cccaaatcga tgccggacgc tatgtagatt tattggaaat
    14161 cgatgccgct tccaacacag gcatcgacaa catccgcgaa gtattggaaa acgcccaata
    14221 cgcgccgacc gccggcaaat acaaagtcta tatcatcgac gaagtgcata tgctttccaa
    14281 aagcgcgttc aacgctatgc tcaaaacgct ggaagagcca cccgaacacg tcaaattcat
    14341 ccttgccacg accgatccgc acaaagttcc cattaccgtc ttgagccgct gcctgcaatt
    14401 cgtcttgcgc aatatgactt cgcagcaggt tgccgaccac ctcgcccacg tcctcgacag
    14461 cgaaaaaatc gcctacgaac cccccgccct gcaacttttg ggacgtgccg ccgccggatc
    14521 gatgcgcgat gccttgagcc tgctcgacca agccatcgcc ctaggttcgg gcaaagttgc
    14581 cgaaaacgat gtccgccaaa tgattggcgc ggttgacaaa caataccttt acgaactgct
    14641 gacaggcatc atcaaccaag acggcgcagc cctgaccgcc aaagcgcagg aaatggcggc
    14701 gtgtgccgtc ggctttgaca acgccttggg cgaactggcc atactgctgc aacaactcgc
    14761 cctgatacag gcagtgccga atgccttggc gcacgacgac cccgattccg atattttgca
    14821 ccgcctcgcc caaaccataa gcggcgaaca aatccagctt tactaccaaa tcgccgtcca
    14881 cggcaaacgc gacctgggcc tcgcccccga cgaatacgcc ggctttatga tgaccctgct
    14941 gcgtatgctg gcgtttgcgc ccttggcagc agcatcgtgt gatgcaaacg ccgtgattga
    15001 aaataccgaa cttcaatccc catcggcaca aaccgccgaa aaggaaaccg ccgcaaaaaa
    15061 gccccaaccg caccctgaag cggaaaccac ccaaacgccc gttcagacgg catccgcagc
    15121 ggcaatgccg tctgaaggca aaactgccga acccgtttcc aatcaagaaa acaacgatgt
    15181 tccgccttgg gaagacgcgc cggacgaagc ccaaaccgca gccggcacgg cgcaaacgtc
    15241 ggcaaaaagc attcagacgg catccgaagc cgaaacatcg cctgacaatc aagtttccaa
    15301 gaacaaggca gccgacaacg aaaccgagac ttccttgtcc gaagtgccgt ctgaaaaccc
    15361 cattcaggca acaccgaatg atgaagccgt tgaaacggaa gcatttgcgc acgaagcccc
    15421 tgcagaacct ttctacggtt acggctttcc ggataatgac tgccccccgg aagacggcgc
    15481 agaaatcccg ccgccggatt gggaacacgc cgcccctgcc gatgcggaag aagaaaacaa
    15541 cgccgacgaa ggcagcaacg acgaaaacaa cacgccgtcc gccccgccgc ccgaattttc
    15601 caccgaaaac tgggcagcca tcgtccggca tttcgcccgc aaactcggcg cggcgcaaat
    15661 gccggcgcaa cactccgcgt ggacggaata ccatcccgac accggcctga tggttttggc
    15721 gatgaccgcc gaagcccgcg ccaccgccga caaaaaacgc ctcgacaaaa tccgcgacac
    15781 ccttgcccaa gcctacgggc tgcaactcac cctgcaaacc caagactggc gtgacgaagc
    15841 cggcagagaa acccccgcga tgcaggacaa gcgcgtccaa gccgaagaca ggcaaaaagc
    15901 acaagcattg ctcgaagccg accccgccgc acaaaaaatc ctccaagcat tcggcgcgca
    15961 atggcagccc gaatcactgg aattggcggc aaaccggcca taaacagata taatgccgcc
    16021 cgaacccttc ggacggcatt gccgtttccc ttattcaatc aaaacagaca ggagtattca
    16081 gtatgttcgg aaaagccgga ttaggcggcc tgatgaaaca ggcgcagcaa atgcaggaaa
    16141 atatgaaaaa agcgcaagcc aaacttgccg aaaccgaaat cgaaggcgaa gcaggcaacg
    16201 gcttggtcaa aatcacaatg acctgcgcgc acgaagtacg caaaatcgac atcagccccg
    16261 atttgattca agaagccgcc gacgacaaag aaatgctcga agacctcatc ctcgccgccc
    16321 tcaaatccgc ccgtgacaaa gccgaagaaa ccgcaaacaa aacaatgggc gcattcgccc
    16381 aaggactccc ccccggagtg ggcgacttct tccgctgatc cccgaccgtc attcccacgc
    16441 aggcgggaat ctagaacgta gaatctaaga aaccgtttta ctcgataaat ttccgtgccg
    16501 acgggtctgg attcccgcct tcgcgggaat gacggcatta atttgcagga ttcggcgtga
    16561 acggtaaaaa cagtgagaat gataagaatg caaaaacggc aagaatagcg ggaatcggca
    16621 ggctgaagcc caccctacca ttatttacac atccgtaccg cttaaatgcc gtctgaaatt
    16681 tcgtcattcc catgaaaacg ggaatccaac cccgtcggag cagaaactta caccccgtca
    16741 ttcccgcaaa agcgggaatc cagtaaccga aaaaccacgg gaatctatcg gaaaaaaaca
    16801 gaaccccccg ccgccgtcat tcccgcgaac gcgggaatct agtaaccgaa aaaccacggg
    16861 aatctatcgg aaaaaacgga aacccccgac cgtcattccc gcgaacgcgg gaatctagaa
    16921 cgtagaatct gagaaaccgt tttactcgat aaatttccat gccaacaggt ctggattccc
    16981 gccttcgcgg gaatgacggc atattttttg catttgatat aaagggtcgt ttgaattttg
    17041 ttcagcaagt gcaaagtgtt gcacataaaa gggcgtaggc ttcatccgta aagtcggcgc
    17101 aggatagagg caaagcgggc gtaggtcggg ctgtagcaac tgtatttttc accccgtcgg
    17161 gcaaaaatac caaaactcaa atcaagccgt tcggaggcag ctcaaaaaac ggtacttcgc
    17221 aacagaagta ccgtttatcg ggattttaag ttttattctt ctgggcgttc gccgtcggtt
    17281 tcgtccaatt ttccttcggt gatgtgcatt tctacgccgt tgagggcgcg aatttttgcg
    17341 tcgatttcgt cggcaatttc gggattttcc ttcagccaga cgcggacgtt gtctttgccc
    17401 tgaccgattt tcgcgccgtt gtagctgtac cacgcgccgg atttgttgat gatgtcgttt
    17461 ttcacgccga tgtcgattaa ttcgccttcc caactgatgc cttctccgta gaggatgtca
    17521 aactctgcct gacggaacgg gggggcgact ttgtttttga tgactttgac gcgggtttcg
    17581 ttgcccaata cttcttcgcc ttttttgatg gatccggtgc ggcggatgtc gaggcggacg
    17641 gaagaataga atttcagcgc gttgccgccg gtggtggttt cggggctgcc gaacattacg
    17701 ccgatcttca tccggatttg gttgatgaac acaaccagcg tgttggtttt tttgatgtgt
    17761 ccggtcagtt tgcgcaaagc ctgactcatc aggcgcgcct gcagtccgac atggctgtcc
    17821 cccatatcgc cttcgatttc ggctttgggg acgagtgcgg ctacggaatc gacgactacc
    17881 atatctatgc cgcccgaacg gacgagtgtg tcgcagattt ccaaagcctg ttcgccggta
    17941 tcgggctggg acaggtaaag ctcttcgact tttacgccga gtttgcgggc gtaaacggga
    18001 tcgaaggcgt gttcggcatc gacaaaggcg cacacgccgc cgtttttctg gcattgggcg
    18061 acggcttcga ggcagagggt ggttttgccg gaggattcgg ggccgaagat ttcgacgatg
    18121 cgcccgcgcg gcagaccgcc gactccgagg gcgaggtcta atccgagcga tccggtggaa
    18181 atgacttcga ggttttcttc ctgctggctg ccgtccattt tcatgatggc gcctttgccg
    18241 aaactttttt cgatttgcgc cagtgcggcg gcaagggctt tgcttttgtc gtctgacatt
    18301 ggggttactc cggaacaaat gcggtatgtg ggatgcggcg caacacgggc tgcggcgcgg
    18361 gatgtgtatc gttttcccga tgtgcgggct atcggtaatg ctgcttcacg gggttgccat
    18421 tatcgcatat ttccttgctt gccgatatgc ggcaggacgc ggcggcttgt gccggaatgg
    18481 aatctggatg ccgtctaaaa ggcggccggc tttgttataa tggcggctgt tttttctgta
    18541 tgtgcctgtt ttatgtgttc ctgccttgtt gtcaaaaata ccgttatcgg aagcggacgc
    18601 accaaaatcg ccgtgccgct tgtcgcccgc gatgccgccg tactttccgc cgtacttgac
    18661 caaatcaaaa acctgccctt cgatatcgtc gagttccgcg ccgatttttt ggaatgcgcg
    18721 ggcagcatcg gcgaagtatt gcgccacacg cagaccgtcc gcgacgcgct gcccgacaag
    18781 ccgctgctgt ttacgttcag acggcatggc gaaggcggcg cgttcccgtg ttcggacggt
    18841 tattatttcg aactgctcga cgcgctgatt gaaagccgcc tgcccgacat tatcgatatc
    18901 gagctgtttg ccggcgaaac cgccgtccgg cgcgccgtgg caaatgctca aaaaaacggc
    18961 atcgccgccc tgctctgcaa tcatgagttt caccgcacgc cgccgcaaga agaaatcgta
    19021 tgccgtctga aacagatgga ggactgcggc gcggacatct gcaaaatcgc ggtgatgccg
    19081 caaagtgcgg aagatgtgct gactttgctt tccgccacac tcaaagcgaa acagcttgcc
    19141 gccaaaccgc ttgtgacgat gtcgatggga cagacggggg cggtcagccg gcttgccgga
    19201 caggtgttcg gctcaagcat cacattcggt tcgggaacgc aaaactccgc gccggggcaa
    19261 atcggcgtat ccgccctccg tgcggcactc gactgcctcg aaaacggagc agactgattt
    19321 cagacagcat caaaacatga tgaaactcaa tccccaacag ctcgaagccg tccgctacct
    19381 cggcggccca ctgctcgtcc ttgccggtgc aggcagcggc aaaaccggcg tgattactca
    19441 aaaaattaag catttgattg tcaatgtcgg ctacctgccg cataccgttg ccgcaattac
    19501 ctttaccaac aaagccgctg cggaaatgca ggagcgcgtt gccaaaatgc tgcccaaacc
    19561 gcaaacgcgc gggctgacga tttgcacgtt ccactctttg ggcatgaaga ttctgcgcga
    19621 agaggcgaac catatcggtt acaaaaaaaa cttctccatt ctcgattcta ccgacagcgc
    19681 gaaaatcatc ggcgaactct taggcggtac gggcaaagaa gccgtattca aggcgcaaca
    19741 tcaaatctcc ctgtggaaaa acgatctgaa aacgcctgaa gatgtcgttc agacggcctc
    19801 taatgcgtgg gaacaacaaa ccgcacgcgt gtatgcgagc tatcaggaaa ccttacaaag
    19861 ctatcaggca gtggacttcg acgacttaat ccgcctgcct gccgtgctgt tgcagcaaaa
    19921 cagcgaggtg cgcaacaaat ggcagcggcg gctgcgttat ctgctggttg acgaatgcca
    19981 agatacgaat acctgccaat ttacgttgat gaaacttctg accggcgcgg aaggtatgtt
    20041 taccgccgtc ggcgacgacg accagtccat ctacgcatgg cgcggcgcaa acatggaaaa
    20101 tctgcgcaaa atgcaggaaa actatccaca gatgaaggtc atcaaactgg agcaaaacta
    20161 ccgctccacc gcgcggattc tcaaaatcgc caacaaagtc atcgagaaca accccaagct
    20221 gttcaccaaa aaactttggt cgaaattggg cgaaggcgag cccgtcaaag tcgttgcctg
    20281 ccaaaacgag cagcacgaag ccgactgggt cgtcagccaa atcgtcaaac aaaaactcat
    20341 cggcggcgac aaaacccaat atgccgattt cgccgtgtta taccgcggaa agcatcaggc
    20401 gaggattttc gaggaagcat tgcgcggcgc gcgcatcccc taccgtctct ccggcggaca
    20461 aagctttttc gacaaagccg aaatcaaaga cgtgttgtct tatgtgcggc tgcttgccaa
    20521 ccccaacgac gatcccgcct ttctgcgtgc cgttaccacg cccaaacgcg gcatcggcga
    20581 tgtaacgctg ggcaagctca acacttacgc gcacgaacac gaatgcagcc tgtatgaagc
    20641 cgcgcaaaac gaagaagccc tcgccacgct gaacaatacc aaccgccaac acctgcaagc
    20701 ctttatggat atgttcggca gttaccgtgc caaagccgaa accggcgaag cgggcgagtt
    20761 catcaacagc ctgctcgaag aaatcgacta tgaaaaccac ctgcttgcca gtgaagaagg
    20821 caaagctggc gaaatcaaat ggcgcaacgt cggcgatttg gtatcatggt ttgcgcgaaa
    20881 aggcggggaa gacggcaaaa acatcatcga actcgcccaa accgtcgcct tgatgacgct
    20941 tttggaagga aaagacgaag aagaaaccga tgccgtctcg ctatccacgc tacacgccgc
    21001 caaaggtttg gagtatccgt atgttttcct tgtcggttgc gaagaaggcg ttttgccgca
    21061 caacgacagc atcgaagaag gcaacgtcga agaagaacgc cgcctgatgt acgtcggcat
    21121 cacccgcgcc aaacgccaac tcacactgac ccactgcgtc aaacgcaaaa aacaaggcac
    21181 atggcagttc cccgaaccca gccgattcat agacgaaatg ccgcaggaag atttgaaaat
    21241 cctgggtcgc aaaggcggcg agccgattgt cagcaaagaa gaaggcagac gcaaccttgc
    21301 cgatataatc ggaaggctcg acaacctaaa aaaaagcggc gcggcggatt aaaccggagc
    21361 cgcaatgccg tctgaaggct tcagacggca tattttttgg acggcgcgcg taaagcggtt
    21421 tacgcccaca aatcctgctg ctggtttttc ggcacaagat gccccacgcc gataccgata
    21481 aggcggaacg cgtcttccgt ctgcggcggg acgcgcgcca tcaacatttg cgcagcctgc
    21541 agcagagctg cgcagtcggg caatacggag gaataagtca gtgtgcgcgt gatgatgcgg
    21601 aaatcgtagg tcttcagctt gagcgttacg ctttgggctt cgacgttttt gcgcgtgatt
    21661 tgccgccaca agtcttcggc aagatggggg aggtgtccgg cagcctgctc gagcggcagg
    21721 tcttcgggca gggtaatttc tgtggagatt tggaggcgtt cgcgttcggc tttgacgggg
    21781 cgttcgtccg taccgcgcac caaatcatag aggcggtatc cgtagcgtcc gaaatggttt
    21841 aagagttcgc cgcgctcgaa acggcgcaag tcgcccgccg tccgcatacc cagcgactgc
    21901 atttttttca gcgttacctt gcccacgccg gggattttgc ccaaaggcag ggtttccaaa
    21961 aatgccatga ctttgtgcgg cggcaacaca aactgcccgt tcggcttgcg ccagtccgac
    22021 gcgattttcg ccagaaattt gttcggcgcg atgcctgcgg atgcagtcaa acctgtttcc
    22081 gcaaaaatgg cggcacggat ttctttggca acttcactgg cgtaagggat gtttttgaaa
    22141 ttacgggtaa cgtcaagata ggcttcgtcc agcgacaagg gttcgattaa atcggtataa
    22201 cgcctgaata cggcgtgaat ctgcgcggaa acctgacggt acaaatcgaa atgcggcggc
    22261 acatacaccg cttgcggaca cagccttttc gccgttgcca ccgacatcgc ggaatgcagc
    22321 ccgaactgcc gtgcctcata cgatgcggcg caaatcaccg aacgcgcgcc ctcccacgcg
    22381 acgaccaccg gccgcccttt caaatgcggc tgttcgcgca gctctaccga tgcgtagaat
    22441 gcgtccatgt cgatgtggat gattttgcgt gaagacatcg gctctgctga ggataaaaag
    22501 gatattctac tgccggcatc gggcaaattc caa
//
LOCUS       DAWFIE010000024        22478 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWFIE010000024 DAWFIE010000000
VERSION     DAWFIE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22478
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.024"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            253..795
                     /locus_tag="WGQ71_000882"
     CDS             253..795
                     /locus_tag="WGQ71_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0706928.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVVTLLNNFYGQKLTEEDVL
                     KKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKWWRAY"
     gene            925..2586
                     /locus_tag="WGQ71_000883"
     CDS             925..2586
                     /locus_tag="WGQ71_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ0706929.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENLGVEIYPGFAAAEVLYHKDGSVKGIATGNM
                     GVGKNGEPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGMKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIAYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMINVNYKEYASPETRYCPAGVYEIIE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            2664..2768
                     /locus_tag="WGQ71_000884"
                     /pseudo
     CDS             2664..2768
                     /locus_tag="WGQ71_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2825..3319)
                     /locus_tag="WGQ71_000885"
     CDS             complement(2825..3319)
                     /locus_tag="WGQ71_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ0706930.1"
                     /translation="MKKTLLAIVAVFALSACRQAEEGPPPLPQQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVSFDDMPDT
                     YIFK"
     gene            complement(3536..4156)
                     /locus_tag="WGQ71_000886"
     CDS             complement(3536..4156)
                     /locus_tag="WGQ71_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0706931.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            complement(4214..5443)
                     /locus_tag="WGQ71_000887"
                     /pseudo
     CDS             complement(4214..5443)
                     /locus_tag="WGQ71_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            complement(5449..5874)
                     /locus_tag="WGQ71_000888"
                     /pseudo
     CDS             complement(5449..5874)
                     /locus_tag="WGQ71_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            complement(6150..7397)
                     /locus_tag="WGQ71_000889"
     CDS             complement(6150..7397)
                     /locus_tag="WGQ71_000889"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ0706932.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEIRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHEYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIRALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSIQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            complement(7549..7935)
                     /gene="gcvH"
                     /locus_tag="WGQ71_000890"
     CDS             complement(7549..7935)
                     /gene="gcvH"
                     /locus_tag="WGQ71_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ0706933.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNDDLPSAPETANSDPYGAGW
                     FFKIKPANPADYDGLLTAEQYAGEVD"
     gene            complement(8097..9197)
                     /gene="gcvT"
                     /locus_tag="WGQ71_000891"
     CDS             complement(8097..9197)
                     /gene="gcvT"
                     /locus_tag="WGQ71_000891"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ0706934.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVHPCGLGARDTLRMEAGMNLYGNDMDDNTSPLEAGMGWTVDLKDESRDFVGKTALLA
                     LKEKGVAVKQVGLLLDKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEVDVRVLKLPFVRNGQKQFD"
     regulatory      complement(9408..9505)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9709..10191
                     /locus_tag="WGQ71_000892"
     CDS             9709..10191
                     /locus_tag="WGQ71_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ0706935.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     gene            10238..10843
                     /locus_tag="WGQ71_000893"
     CDS             10238..10843
                     /locus_tag="WGQ71_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ0706936.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIVQIAPFHPDFQFEGTDSDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            10840..11199
                     /locus_tag="WGQ71_000894"
     CDS             10840..11199
                     /locus_tag="WGQ71_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ0706937.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            11559..12980
                     /locus_tag="WGQ71_000895"
     CDS             11559..12980
                     /locus_tag="WGQ71_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ0706938.1"
                     /translation="MRHLPLFSLILFPASVYAADLDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGSSAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            13378..14721
                     /locus_tag="WGQ71_000896"
     CDS             13378..14721
                     /locus_tag="WGQ71_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ0706939.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPETLANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKQGLLVLSRLTERKIHVCKAVGADVPSENAANIEIHEFGGPHPA
                     GLSGTHIHFIEPVGANKIVWTINYQDVIAIGRLFVTGRLNTERVVALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LLGWVAPQPDKYSITRTTLGHFLKNKLFKFNTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            14724..15956
                     /locus_tag="WGQ71_000897"
     CDS             14724..15956
                     /locus_tag="WGQ71_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ0706940.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYTLAD
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFAVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            15949..16725
                     /locus_tag="WGQ71_000898"
     CDS             15949..16725
                     /locus_tag="WGQ71_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ0706941.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKNTDISKTFAERIEQRVVDLATGEYVADAPKDFSARVAGKDPA
                     QSIQIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQVILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            16725..17351
                     /locus_tag="WGQ71_000899"
     CDS             16725..17351
                     /locus_tag="WGQ71_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ0706942.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            17355..17948
                     /gene="nqrE"
                     /locus_tag="WGQ71_000900"
     CDS             17355..17948
                     /gene="nqrE"
                     /locus_tag="WGQ71_000900"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ0706943.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            17962..19179
                     /gene="nqrF"
                     /locus_tag="WGQ71_000901"
     CDS             17962..19179
                     /gene="nqrF"
                     /locus_tag="WGQ71_000901"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ0706944.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            19331..20386
                     /locus_tag="WGQ71_000902"
     CDS             19331..20386
                     /locus_tag="WGQ71_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0706945.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     TGGYRTAMSSEFEKLLR"
     gene            20412..20627
                     /gene="nqrM"
                     /locus_tag="WGQ71_000903"
     CDS             20412..20627
                     /gene="nqrM"
                     /locus_tag="WGQ71_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ0706946.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRTIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(20751..21329)
                     /gene="grpE"
                     /locus_tag="WGQ71_000904"
     CDS             complement(20751..21329)
                     /gene="grpE"
                     /locus_tag="WGQ71_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ0706947.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            21512..22330
                     /gene="cysE"
                     /locus_tag="WGQ71_000905"
     CDS             21512..22330
                     /gene="cysE"
                     /locus_tag="WGQ71_000905"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ0706948.1"
                     /translation="MKKNHLNTTGFDLWHTIREETAAAAAAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDEQISKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHWLYLNGRKTLAYFLQNRMSEVFGVDIHPAAHFGHGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFAEIDFMI"
BASE COUNT         5321 a         6122 c         5633 g         5402 t
ORIGIN      
        1 ggatttttgc tttcgtgggg atgacgggat tttaagttac ggtcatttat cggaaaagca
       61 gaaactgctc cgccgttatt ccagcgaagg cgggaatctg aacacgtccg cacggaaacc
      121 tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta cagatagtac
      181 ggcaaggcga ggcaacgccg tactggttta aatttaatcc actataatcc tgttgcttat
      241 ggaaaaatca aagtgcagag ttggaaagaa aggcgcgatt tcaatattgt aaagcaggat
      301 ttggattttt cctgcggggc ggcttcggtg gtgacgcttt tgaacaattt ttacgggcaa
      361 aagctgacgg aagaggacgt gttaaaaaaa ctggataagg agcagatgcc cgcgtcgttt
      421 gaggatatgc ggcgcattat gcctgatttg ggttttgaag cgaaaggcta tgccctgtct
      481 ttcgagcagc ttgcacagtt gcaaatcccc gtcatcgtgt atctgaaata ccgcaaggat
      541 gatcatttct cggtattgcg cggtatagac ggcaatacgg ttttgcttgc cgacccgtcg
      601 ctgggtcatg tttcaatgag cagggcgcag tttttggatg cttggcaaac ccgtgaggga
      661 aatttggcag gtaagatttt ggcggttgtg ccgaaaggga gggatgccgg cggggataag
      721 gcgtttttca cacgcagtcc gaaacgtcag acggagtttg cggtcggaca ggtaaaatgg
      781 tggcgtgctt attgaaattt cgacaaaggt cgtctgaaaa ccgaaaatat ggttttcaga
      841 cgacctttgt tgtatttggt aactatatgt tcccgttgta taattacgga tttgcaattc
      901 aataataaat acacaggaac cgccatgaca gaatccatca cccgcgacag tatgcaatac
      961 gatgtcgtga ttgtcggcgc aggcccgtcg ggtttgtccg ccgccatcaa actcaagcag
     1021 cttgccgaac agaacgggcg cgaaatcagc gtttgcgtgg tggagaaagg ttcggaggcg
     1081 ggcgcgcatt cgctggcggg cgcggtcatt gacccgattg cgctgaatga gttgattccc
     1141 gactggaaag aaaaaggcgc gccgctgacg cgcacggtaa cgcaggacaa ggtgttgttc
     1201 ctgacggaga aaaaagcgtt caatctgccc attaccccca atttcgacaa tcacggcaac
     1261 tacatcgtca gcttgggcga agtcgtgcgc tggttggcgg agcaggcgga aaatttgggc
     1321 gtggaaattt atccgggctt tgccgctgcc gaagtgctgt atcacaaaga cggttcggtc
     1381 aaaggcattg caaccggcaa tatgggcgtg ggcaaaaacg gcgagccgac cgattcattc
     1441 cagcccggca tggagctttg ggcacggcaa accctgtttg ccgagggctg ccgaggttcg
     1501 ctttccaaac aaatcatcga acgcttcaaa ctcgaccaaa acagccagcc gcaaacttac
     1561 ggcttgggca tgaaagaagt ttgggaagtg ccgtccgaac agcatcagcc cggtttggtg
     1621 gtgcacagcg caggctggcc gctggacagc aaaacctacg gcggcgcgtt tgtttaccat
     1681 ttcgacgaca acaaagtcgc cgtcggcttc gtggtcggtt tggactatca aaatccttat
     1741 ctgtcgccgt ttgaagagtt ccaacgtttc aaaacccatc ccgaaatccg caaaaccttt
     1801 gaaggcggcc gccgcattgc ttacggcgcg cgttcgctga ttgaaggcgg tttgcaaagt
     1861 ttgccgaaac tctcgttcaa agggggcatt ttggtcggcg atgcggcggg tttcctcaat
     1921 atgccgcgca tcaaaggcat tcataccgcg atgaaatccg ccatgctcgc cgccgaagcc
     1981 gtgttcccct tgttggaaaa ccttgaagaa gtggagggtt tcgacagcgg caaagaggcg
     2041 gcggattatc agcaacgttt tgaacaaagc tggctgtatc aagagcttta cgccgcgcgc
     2101 aacgtccgtc cgtcattcaa atggggcgtt tacctcggct cgctttatac cggcatcgac
     2161 cagatgattt tcagaggcaa agccccgtgg accttgaaac atcacggtaa agacaacgag
     2221 cagctcaaaa aagccgccgc gtgcaagccg attgcttatc cgaaacccga tggcgtgttg
     2281 accttcgacc gcttgagcag cgtcttcctt gccaatctcg cgcacgaaga aaaccagccc
     2341 gaccatttgg tgctgaacaa tccgcaaacg atgataaacg tgaactacaa agaatacgcc
     2401 tcgcccgaaa cgcgctattg tccggcgggt gtgtacgaaa ttattgaaga aaacggcaat
     2461 ccgcgcctgc aaatcaacgc ggcaaactgc gtgcactgca aaacctgcga catcaaagac
     2521 ccgacgcaaa acatcacttg gatttgcccc gaaggcgcga gcgggccgaa ttacggcggg
     2581 atgtaaacct tattccgccg ttacgcccgg tcaggaataa ggaatggggc agttgttcaa
     2641 acctgccccg tttgaaccca taatatagtg gattaaactt aaatcaggac aaggcgacga
     2701 agccgcagac agtacaaata gtacggcaag gcgaggcaac gctgtactgg tttaaattta
     2761 atccactata aaaaatgccg tctgaacctt ggcttcaacg ttcagacggt atttttgttt
     2821 ggatttattt gaaaatatag gtatcaggca tatcgtcgaa actgacaacc ttaccgcctt
     2881 tatcctttgc aaatttttca gcctgctcct tgttgccgaa cggtagcgcg tcttccgcac
     2941 ccataccgcc gataaagccg ctgtcgatga cgtaaaaggc ttttttcgca tccatccact
     3001 ccgtgtcggc attgggattc gtccaatcgg taacattgcc catatcggta acgtaaatca
     3061 cgcggatgcc tttaggctct tcgggcagct tggtatagcc gaacatctgc ttgatggtgg
     3121 agaaccaaac gggctgatcg ggtttgccgt tcaaaaaaat ctgggcttta gggccgttgt
     3181 gttcggtcag attcatactg caatagtgtc cgaccgaacg gtcgctaatc tgctggggta
     3241 aaggcggcgg cccctcttcc gcctgccggc aggcacttaa ggcgaaaacg gcaacaattg
     3301 ccaacagggt tttcttcata tttgtcgctt tctaaaaatt ccggctgcca aaacaagcgg
     3361 aatgataacc cataaaacct gcgcggtcag caaaacgggg acggtcaggc tgatttctcc
     3421 gctcaaaccc gccatacccg catatagtgg attaacaaaa atcaggacaa ggcaacgaag
     3481 ccgcagacag tacaaatagt acggcaaggc gaggtaacgc cgtactggtt taaatttaat
     3541 ccacgataat gcggtcggcg tgcccgtcca actcggcaag ggcgtgggtg ctgagcaata
     3601 cggtcgcgcc gcgcccgttg agttcgcgca cgacttcgta aaacatttgt cgtgatgcag
     3661 ggtcaagacc ggttgtcggt tcgtcaaaca gcaggacttt gggctcgccc agcagggctt
     3721 gtgccaaggc aaggcgttgg cgcatccctt tcgagtaggt gccgacgcgg cggtgtgccg
     3781 cctgtgaaat accgacgcgc tcgagcagcc cccggttctg cgtgagcggc tgttttttaa
     3841 gtttggcgta aaaatccagc gtttcgatgc cggtcagcga agggtgcagc gcaacagttt
     3901 cgggcaggta gccgatttgg ctgcgaagcc gcgcccccgc tttgctgccg gtacgttcgc
     3961 ccaaaagcat cacttcgcct tcggtcgggg taatcagccc gagtatcagc ttcatgatgg
     4021 tggacttgcc cgcaccgttg tgtccggcaa gcccgacgct ttctcctgcc ttcaaaacca
     4081 aatcgacttg gttgacggct ttttgcgcgc cgaagcgttt ggttaccttc ctcaattcga
     4141 catgatgtgt ggtcatggtt gttccttttg tgttttcaga cggcatttga atccggatgc
     4201 cgtctgaagc agactagttc aaagaaccgt tttccgccct gccccattcc gactgccgcg
     4261 tttcggcttc tttgagcagc ccgtccttca tcgcctgata acgggtttga attttgggga
     4321 cataaggctt catcagcggt ttgctgtcca ccacgccgcc gggcagaacg gcgggaaact
     4381 gcgcctgcgc ccatttgacg atgctgattg cgagactgtt catcaagaga cgcgatacgg
     4441 gcgcgcgcca gatgatttgg tcgatgatgc cgttgggccg gtacgcgctg tcgccgaagc
     4501 cgtcgccgtt caaatcgaac gcactgttgt cgctccaata gttgccgtgt ccgccctcgc
     4561 tccagtcgag aaagcgcgtg ctgacgtatt tgacctggct ttcgttgttg ataaaggaat
     4621 tgtcgtgcag agacgtgcct tcgatggcgg cggtaaagtg tatgccgatt tggcagtttt
     4681 caaaatgatt ggcggacagt ttatcgtagt tggcattgta ggcaaaaacg cacttgcccg
     4741 ctttgttgat aatattgtcg tgaatatcgg aatagttgac atagttgagc atgatgccct
     4801 agtcgcgact accgacggca atattgtcga atactttgag ccgttcggaa aacatcagca
     4861 catagcccat attgttgccc acggaaatat tgccgctgac ttcgctgtcg ttggtgtaca
     4921 tatagtggac ggcgaaacgc aaatcgctga agcggttgtt tttataggtg ttgtgcgtgc
     4981 tggtgttgga aaaaatgccg tcccgccctt tggaaatgtc gttgccgacg acctgcgcac
     5041 cgggtgcgtt ccaaacggta acgccgttgc cgcgctcgtt cacgcgcaaa gtcgcgtcgc
     5101 cgacgatttt attctcccgc accatcgcat cggcagaacc atgcagatag acgccgaccg
     5161 aattatccaa aatattgttg tgttcaatca gggcgcgcgg agcagtttct tcgagataaa
     5221 taccggcatc cattgctggc aggctcatac cggaacgtgt aacggtcagg ttgcggagcg
     5281 ttacgtccgg cgcatgcaca gctatcgtac gtccgctcct gtctccttcg atggttgcag
     5341 aacggtcagc aggcccttca atcgtaatcg gtttgtcgat ataaagtttg gtttgatata
     5401 cgccggaagc cagtttgagc gtatcgcccg catgggcgcg ggcaaatgcg ggataccgat
     5461 cagcataatc gactcatggt gatcaaccaa tttcgccccg gctatcgtgc cgtcgtttgc
     5521 caacgccatc agcgtatcga tcggcttgct cgaataaccg cgcgtattga ccacatcggt
     5581 tgtgatatag accaagccca actgctcatc gcctttgtaa acgcgggcaa ccataggctt
     5641 accttccggc ttgccgtaac gatccgcacc cggaaaaatt tccgaaggct gtattttcgc
     5701 cagaaaatca ggcagacgct ccgcataagc gggcagtgcc gccgtcaaaa agacaaaggc
     5761 ggcaaaaaac gccgttatcc gcgcccgaaa cgcggacata cgtttgattg aaaaacagga
     5821 catgactatc cccgatatac cgatactctt taatattgat attatatgaa tcatctatcg
     5881 gcgatttttt gatgcaaatt aaatttacaa atcttaaaac caatccgcat acgggataat
     5941 atagtggatt aaatttaaac cagtacagcg ttgcctcgcc ttgccgtact atctgtactg
     6001 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatactttg tgattacatc
     6061 gatataatgt gggatttcac gatgttgtca ggcggcacaa aaaaatgccg tctgaaacct
     6121 cttcagacgg catttttccc ggcgcaccgt tatttgtcca aatgataaat ctgcgcgacg
     6181 gcatgaacca aatctttatc ttcttccccc gccttattca aagtttgggt cggcgaatgc
     6241 agcagcttgt tggtcagttg gatcgacagc ctttccaaaa cctcttctgc cgttgcgcct
     6301 ttggcaagct gtttcatcgc attttccaag acctgtttgc gcgctttctc tccctcatcc
     6361 ctcaatgccc tgattaacgg gacactctgc ctgccctgct gctgcctgac aaattcggca
     6421 accttctcgg acaccagcgt ttcggcggcg gcggcggcct tctgccttgc ctccttgccg
     6481 ctttggacga tattgaccat atcgtccacc gtataaagat aggcatcgtt caaatctccg
     6541 acttccgcct caatgtctcg cggcacggcc aagtcaagca taaacaacgg catactctgc
     6601 ctttgtttca atgcgcgctc caccatacct ttgccgacaa tgggcaactg gcttgccgtt
     6661 gaagaaacca ccacgtcgta ctcatgcaaa atggcaggca gatcggacag caggcacggt
     6721 tcggcgttga caccgagctt gtcgcacaac tcctgtgcac gcgccagcgt ccggttggca
     6781 accgtcatca gccggggact tttggcggca aaataagtgg caaccagctc aatcatctca
     6841 cccgcaccga taaacaagac attcaaatcg ccgatgtcgg ggaaaatctg ctctgccaac
     6901 ttgacggaag cggaagccat ggaaaccgag ttctcgccga cagcggtatc ggtacggatc
     6961 tctttagcaa cggaaaaggt tttttggaac agggcattga gtttcttacc catactttcc
     7021 tgctcttgag caaccctgac cgcatcctta atctgtccta aaatctgcgg ctcgcccaac
     7081 accatcgaat ccaagccgca ggcgacgcgg aaagcatggc gcacagtctc ctgcatcccc
     7141 aaagtataaa ggtaggggct gatttcttct atgggaaggc tgtgatagtc tgcgagccaa
     7201 cggatgattt cttccgaatc gcctacacag taaagctcgg tgcggttgca ggtagaaagg
     7261 attaccgcct ccgttgccgc attgcttcgg gcaagattgc ggacggcttc gggcaggcag
     7321 gccgcggcaa acgccagctt ttcccgtatg cttaaaggtg cggtttgatg attgagtccg
     7381 acagcggtaa gttgcatgaa aaaatcggta aagatgaata tagaccgtta ttatagccta
     7441 actgcttcct taaaacacgg acaaaaaact gcacctccga ccgcaaaaat gccgtctgaa
     7501 ggtgcagttt ccgtttgtcc ggcggttgcc gtcgggcagc cgggcggttt aatctacttc
     7561 gcccgcgtat tgttcggcag tcagcagacc gtcgtaatcg gcaggattgg caggtttgat
     7621 tttgaagaac cagcctgcgc cgtaagggtc gctgttggca gtttcaggtg cactgggtaa
     7681 gtcatcgttg acggcaacga cttcgcctgc aatcggtgcg tacacgtcgg acgcggcttt
     7741 tacagactca accacaccgg cttgctcttc agcggcaagg ttcgcgccga cttcgggcag
     7801 ctcgacgaac acgatgtcgc ccaacagctc ttgcgcgtgg tgggtaatgc cgacggtaat
     7861 cgtaccgtct tcttcaaggc gcagccattc atggctggca acgtatttca gttcggccgg
     7921 gatgttgttg ctcatggttt gatactccat tgataggggt tattttcaga cggcatctgc
     7981 atgaatcaat atccacagat gccttgttca atgctttcta caaacgtccg tatcggacaa
     8041 caattaaaac ctgctttcag acggcatatg aaatgaaaat gccgtctgaa accgcatcaa
     8101 tcaaactgtt tctgtccgtt gcgcacaaac ggcagcttca gtacgcgtac gtccacttct
     8161 ttgccgcgaa tcagaacttt ggcggtatcg ccgtcaaagt ctttcggcac gcgggcgatg
     8221 gcgatggatt gtttcaggct tggagagaat acgccgctgg tggtttcgcc tttgcctttg
     8281 tcggtcaaca cttccatatg cgcgcgcagg atgccgcctt tgtcgagcaa cagaccgacc
     8341 tgtttgacgg caacgccttt ttctttcaat gccagcaaag cggttttacc gacaaaatcg
     8401 cggctttcgt ctttcaaatc aaccgtccaa cccatgcctg cttcgagcgg actggtattg
     8461 tcgtccatat cgttgccgta gaggttcata ccggcttcca tgcgcagggt gtcgcgtgcg
     8521 ccgaggccgc aaggatgtac gcctgctgct tgcagggctt tgaagaatgc aacggcttcg
     8581 gtaccgggca ggatgacttc cacgccgtct tcgccggtat agccagtgcg ggcgacaaac
     8641 cagtcgttgc ccaaatccgc gccttggaac ggtttgaggt tatggacgac atctgcccat
     8701 tcgggtttga cggtcaggag tttttcaatg gctttaggac cttgtacggc gagcatgccg
     8761 aggtcgtagc gcggattgaa ggaaacaccg aactcttgtc cgactttgtg gaattgtgcc
     8821 gtatcttttt cgcgagtcgc gccgttggac acgatgcggt attgggtttc ggcttcgttg
     8881 gtgcggtaaa cgattaagtc gtcaatcacg ccgccgttgt cgttgagcaa agcggaataa
     8941 agggctttgc cgacaaaagc gagcttggcg acatcgttgg caatcaattt gcggaaaaag
     9001 gctttggcat ttgcgcctgc tacgtcggta acaagcatat gggatacgtc aaacataccg
     9061 gcgtcggtgc gcacggcttc gtgttcggcg atttgcgaac cataatggat gggcagctcc
     9121 cagccggcaa aatctaccgg cttcgcgcct gcatcttgat gggcttgatg aaatggggtg
     9181 gttttcagag taatcattct caagtttctc cggatttttt gttcagatgc cacccgcgca
     9241 cctgtgcgcg gatgacccta tctgtccttg aacctgagat tttcggccgt atccgcatga
     9301 tggcaaacca gtctgctcac tgctttgcgc ggttgtctga atggtggttc agacggcctt
     9361 ctccccttcg gtggacgtta ttccaacgcc gctctccaga ttgtttgttt caatgtgcag
     9421 tccctgatac ctgagcgatt taagggtgtc tgcgccttcg gtggttacat tgtagttggg
     9481 tgtaacactc tcctgcacat ttcccgatta tgtcttgaat cggcggtttg ggcaagccgt
     9541 cttaaaagag atgcaaaccg accgcctctg ttttcacatg attttccgaa aacaaaattt
     9601 gtcccggatg acggtataac tttttatatg ttaatatggt ctccatctgt gcggattgcc
     9661 gcaaacatac ggacagacaa tccgaattcc ttaaaacgga acgcaaatat gccccaactc
     9721 actttagaca aaaccgatat taaaatctta caggttctcc aggaaaacgg caggctgacc
     9781 aatgtcgaac tgtccgaacg tgtcgcactt tcgccttctc cgtgcctgcg ccgtctgaag
     9841 cagttggaag atgccggcat cgtccgccaa tatgccgcgc tgctgtctcc ggaatccgtc
     9901 aacctggggc ttcaggcatt tatccgcgtt tccatccgca aagcaaaaga cgcgcgggaa
     9961 gactttgccg catcggtccg aaaatggccg gaagtcttga gctgcttcgc cctgacaggg
    10021 gaaaccgact acctgcttca ggcgtttttt accgatatga acgcgttttc ccattttgtt
    10081 ttggatacgc tcctgtccca ccacggcgta caagatgcgc aatcgagctt tgttttaaaa
    10141 gaaatcaaac acaccacctc cctgccgctc aaccacctgc tgaaggaata gtgcccttcc
    10201 cgattttccc gcacgtgcaa tctgcaaaca catttacatg acaataatga atatcaatac
    10261 ttccgaaaat aaagatgctg ttgccgaaca caccggacaa tggttggaaa aagccgtcat
    10321 cggtctgaac ctgtgtccct ttgccaaagc cccccacgtt aaaaaccttg tccgcatcgc
    10381 gatcagcgaa gccaaacacc ttgacggttt tttggaagac ttggacgaag aactgcagcg
    10441 actgggcaat acacccgcca ccgaactgga aactaccctg ctggttcacc caaccctatt
    10501 ccccgatttc gacgtattca acgatatgct cgacattgcc gatgccgccg ttgtcgaaaa
    10561 cggcttggaa ggcatcgtcc aaatcgcccc gtttcatccc gatttccaat ttgaaggcac
    10621 ggattcagac gacatcggca actacaccaa ccgttctccc tatccgacgc tgcacctcat
    10681 ccgagaagac agcattgcca aagccgcaca agcctttccc gacgcttcgg caatattcga
    10741 acgcaatatc gccctgctgg aaaaaatggg acatgaaggc tgggcaaaac tcggtatcac
    10801 atcctgccct tatccgcaca ataagaaaaa tatttcaaaa tgatacgtta tcttttaatt
    10861 gcctgcggcg gcatctccct gctgttgggg ataatcggca tttttttgcc gctgttgccg
    10921 accacgccgt tcgtactgct ctccgccgcc tgctgggcaa aggcatcccc gcgctttcac
    10981 cgctggctgc accggcaccg ctatttcggt ccgatggttc ataactggga acaaaacggc
    11041 gcagtgccgc gcaaagccaa aatattcgcc atcagtatga tgaccgcatc ctgcctgata
    11101 atgttttggc agtttcccca acgctggtgg gtcggggcgg tttcatcggt tttttgttcc
    11161 cttgtcgcca tatggatgtg gcgcaggccc gaatcttgaa tgccgtactg aaagggataa
    11221 gggcaaacaa acgggggggg caaacctgtt tcattgcaaa tatatgttaa aataaattca
    11281 tttttcgttt tcgatagatt atttattaaa gacatatatt tatttgtttt aaataaaatt
    11341 atttatcaaa aagaacatat attcccaagc acggcaatcc gccttcccgc cgccttccag
    11401 tttgggaaac tggtttatcc tgattattta ctttacaatc aatcggaaaa cttttttcaa
    11461 aaacaaacag gcagcacagt ccgcctatgt ccgggcggca cggtgcctgt atcactccca
    11521 cgcgccgcgc aaggcagtgc ttgaatcgag gaaacacgat gcgccaccta cccctgtttt
    11581 cactcatact tttcccggca tcggtttacg ccgcagattt ggacggcgca aacctaaacc
    11641 tgctgtgggg cttgcccttt gccctgattt tgctgtctat cgcattgggt cccttgtttt
    11701 tttcgcatac ctggcatcac cattacggca aaatcaccgc cttttggaca ttgctcttcc
    11761 tcatcccgtt cagtttggtt tttggctcgt ccgccggtat ccataccgtc gcacacgcac
    11821 tcgttgaaga atacatcccc ttcatcctgc tgctgcttgc cctgtacacc atttcaggcg
    11881 gcattttggt ttggggcgac ttgaacggca cacccaagct caacaccgcc ctgcttgccg
    11941 tcggtacggc acttgcctct atcatgggaa cgaccggcgc agcaatgctg atgattcgtc
    12001 cgctgctgaa agccaaccaa aaccgtaccc gccgcgtgca catcgtcatc ttcttcattt
    12061 tcctggttgc aaacatcggc ggcggtctga cccctttggg cgacccccca ctcttcctcg
    12121 gcttcctcaa aggtgtagat ttcatgtgga cggtcaaaca tatgttcgcc cccgtcctga
    12181 tcagcaccgc tgtcctgttg accgccttct atttcatcga caaccgtttc ttcaaacagg
    12241 aaagcattgc acaagatacg ccggcacaac aggaaaaacc cgaaaaaatc gccatcttcg
    12301 gcaaatggaa cttcctcctg ctttcgggcg tggtcggcgc ggttctaatg tccggccttt
    12361 ggaaacccga acacccggga tttgaaatcc tcggcagccg ttacgccctg caaaacctcg
    12421 tccgcgatgt catcctgatt gcattgaccg ccgtatctat ggcaatcacg cccaaacaag
    12481 tccgcgcagg caacgaattc aactttgaac ccatcgccga agtgggcaaa ctcttcctcg
    12541 gcatcttcat caccatcttt cccgtcctga gcattctgaa agcaggcgag gcaggcgcgc
    12601 tgggcggggt ggtatcgctg gttcacgata cggcaggtca tccgattaat gtgatgtatt
    12661 tttggatgag cggcatattg tcggcattct tggataacgc gcccacttat ctcgtttttt
    12721 tcaatatggc gggcggcgat gcccaagcct tgatgacggg taccctgttt cattcgctgc
    12781 tggcggtttc tatgggttcg gtattcatgg gcgcactgac ctacatcggc aacgcaccga
    12841 acttcatggt caaggccatt gccgaacagc gcggcgtacc gatgccgact ttcttcggct
    12901 atatgatgtg gtcggtcgcc ttcctgacac ccgtcttcat cgtacatacc cttatctttt
    12961 tcgttttcaa actgctgtaa acgctatgcc gtctgaacat tcagacggca ttttaaattc
    13021 cggcataatc aaatcaatat ccccccttcc gacaattata gtggattaac aaaaatcagg
    13081 acaaggcgac gaagccgcag acagtacaaa tagtacggaa ccgattcact tggtgcttca
    13141 gcaccttaga gaatcgttct ctttgagcta aggcgaggca acgccgtact ggtttaaatt
    13201 taatccacta taaaaacggc ttccgcaagg aaaaaagcag caaatctgac aaacaggtac
    13261 aattcctgat agaatctaac actttgctac attaatttat tcaatgcttt tgatgccacg
    13321 cgacacggta tcaaaacacg ccgtttttac cttaaccatc acaagtaatg caaatatatg
    13381 attaaaatca aaaaaggtct aaacctgccc atcgcgggca gaccggagca agtcatttat
    13441 gacgggcccg tcattaccga agtcgcgttg cttggcgaag aatatgccgg tatgcgcccc
    13501 tcgatgaaag tcaaggaagg agatgccgtc aaaaaaggcc aagtgctgtt tgaagacaaa
    13561 aagaatccgg gcgtggtgtt tactgcgccg gtttcaggca aaatcgccgc gattcaccgt
    13621 ggcgaaaagc gcgtacttca gtcggtcgtg attgccgttg aaggcaacga cgaaatcgag
    13681 ttcgaacgct acgcgcccga aacgttggca aacttaagcg gcgaagaagt gcgccgcaat
    13741 ctgattcaat ccggtttgtg gactgcgctg cgtacccgtc cgttcagcaa aatccctgcc
    13801 gtcgatgccg agccgttcgc catcttcgtc aatgcgatgg acaccaatcc gctggctgcc
    13861 gaccctacgg tcattatcaa agaagccgcc gaagatttca aacaaggttt gctggtattg
    13921 agccgcctaa ccgagcgcaa aatccatgtt tgtaaggcag tcggtgcgga cgtgccgtct
    13981 gaaaacgccg ccaacatcga aatacatgaa tttggcggcc cgcatcctgc cggtttgagt
    14041 ggcacgcaca ttcatttcat tgagcctgtc ggtgcgaaca aaatcgtttg gaccatcaat
    14101 tatcaagatg taattgccat cggacgtttg ttcgtaacag gtcgtttgaa taccgagcgc
    14161 gtggttgcct tgggtggttc tcaagtcaac aaaccacgcc tcttgcgtac cgttttgggt
    14221 gcgaaagtat cgcaaattac tgcgggcgaa ttggttgacg cagacaaccg cgtgatttcc
    14281 ggttcggtat tgaacggcgc gattgcacaa ggcgcgcacg attatttggg acgctaccac
    14341 aatcagattt ccgttatcga agaaggccgc agcaaagagc tgctcggctg ggttgcgccg
    14401 cagccggaca aatactccat cacgcgtaca accctcggcc atttcctgaa aaacaaactc
    14461 ttcaagttca acacagccgt caacggcggc gaccgcgcca tggtgccgat tggtacttac
    14521 gagcgcgtga tgcccttgga tatcctgccc accctgcttt tgcgcgattt aatcgtcggc
    14581 gataccgaca gcgcgcaggc attgggttgc ttggaattgg acgaagaaga cctcgctttg
    14641 tgcagcttcg tctgcccggg caaatacgaa tacggcccgc tgttgcgcaa agtgctggaa
    14701 accattgaga aggaaggctg attatgggct tgaaacattt tctggaaaaa atcgaaccgc
    14761 acttcctgcc gggcggcaaa catgaaaaat ggtatgccct ctacgaagcg gcggcgacga
    14821 ttttctacac atccggtgcg gtaacgcgca aagcggcaca cgtccgcgac gcgctcgact
    14881 ccaagcgcat gatgattttg gtgtggctgg ctttgttccc cgccatgttt tacggcatgt
    14941 acaacgtcgg cgcacaggca ttcggtgcct taacgcccga tttgctgcaa caaagcatcg
    15001 ccaacgactg gcattacacc cttgccgacg ctttgggcat caatatgtcg tctgaagcgg
    15061 gcgtgttggg caaaatgctg ttcggcgcga tttacttcct gccgatttac gcgaccgtat
    15121 ttgccgtcgg cggtttctgg gaagtcttgt tcgccaccgt acgcaaacac gaaatcaacg
    15181 aaggtttctt tgttacctcg attctgtttg ccttaatcgt tccgcccacg ctgccgctgt
    15241 ggcaggcggc tttgggtatt tctttcggcg ttgtggttgc gaaagaagta ttcggcggta
    15301 cgggtaaaaa ctttatgaac cctgcgctgg caggccgcgc cttcctgttc ttcgcctacc
    15361 ccgccaactt gagcggcgat gcggtttgga cggcggttga cggctattcc ggcgcaaccg
    15421 cgctggcgca atgggcggca cacggtgcag acggcctgaa aaacgccgta accggtcaaa
    15481 ccatcacttg gatggacgcg tttatcggca aactgcccgg ctccattggc gaagtctcca
    15541 ctttggcact cttaatcggc ggcgcgttta tcgtgtttgc ccgcatcgct tcttggcgca
    15601 ttattgccgg cgtgatgatc ggtatgattg cgatgtcttc gctgttcaac ttcatcggtt
    15661 cggacaccaa cgccatgttt gctatgcctt ggtactggca tttggtcgtc ggcggcttcg
    15721 ccatcggtat gctgtttatg gcgaccgacc ccgtttccgc ttcctttacc aatgtcggca
    15781 aatggtggta cggcgcactg atcggtgtga tgtgcgtatt aatccgcgtg gtcaatccgg
    15841 cttaccccga aggcatgatg ttggcgattc tgtttgccaa cctgtttgcc ccgattttcg
    15901 actatttcgt cgcacaagcg aacatcaaac gcagaaaggc gcgcagcaat ggctaagaaa
    15961 ttcgataaag acagcttcag cggcacgctg attgtcgtgt tggcggtcag cctgatttgc
    16021 tcggtcatcg ttgccggtgc ggtcgtcggc ttgaaaccca tccaagagaa acaaaaactc
    16081 caagacaaac aaggctatat cttgagcgta gccggtttga tggataaaaa caccgacatt
    16141 agcaaaacct ttgccgagcg tatcgagcaa cgtgtcgtgg acttggcaac cggcgaatat
    16201 gtcgctgatg cgcctaaaga cttcagcgca cgcgttgccg gcaaagaccc tgcccaaagc
    16261 atccaaatca aacctgaaga cgatttggca ggcatcaaaa gccgtgccaa atacaccgag
    16321 gtttatttgg taaaaggcga agacggcaaa atcggccaag tcatcctgcc gatgcacggc
    16381 aacggtttgt ggtcggtcat gtacggcttt gtcgccatcc aacccgacgg caacaccatc
    16441 aacggcatta cctactacga gcaaggcgaa accccgggct tgggcggcga aatcggcaat
    16501 cctttgtggc agcaaaaatt cgtcggcaaa aaactgtttg acggacaagg caaactcgcc
    16561 ctgcacgtcg gcaaaggcgc gggttcggac aaagaacacg gcgtagatgc cctctccggc
    16621 gcatcgctga catccaaagg cgtgcaaggt tcgttcgcct actggttcgg cgaaaacggc
    16681 tatatcccct acctgaacaa attgaaatca gcaggagcgc aataatggct gatatgaaac
    16741 gcttgaaaca tttgatgttt tcacccttta tcgacaacaa cccgattgcc ttgcaggttt
    16801 tgggtatttg ttcggctttg gcggttacca ccaaacttca gacggccatc gtgatgggta
    16861 tttccgtagc tttggtaacc ggtttttcca gcttcttcat ttcgctggta cgcaactaca
    16921 tccccaacag catccgtatt atcgtgcaaa tggcgattat cgcgtcgctg gttacgctgg
    16981 tcgaccaact cttgcaggca tttgcctacg aattgtccaa acagctttcc gtattcgtcg
    17041 gtctgattat taccaactgt atcgtgatgg gccgcgccga agcatttgcg atgaaagagc
    17101 cgccgctgga aagcctgatc gacggcatcg gcaacggcgc gggctacggg ataatgctgc
    17161 ttgtcgtcgc caccgtccgc gaactgattg gctcgggcaa actcttgggc tacaccgttt
    17221 tccaaaccgt gcaggacggc ggctggtatc agaccaacgg cttgttcctg ctcgccccca
    17281 gcgcgttctt catcatcggc tttttgattt ggggactgcg tacctggaaa cccgaacagg
    17341 cggaggaata agctatggaa cactatttga gcctcttcat caaatccgtc ttcattgaaa
    17401 atatggcact atccttcttt ttgggtatgt gcacgttttt ggcggtatcc aaaaaagtat
    17461 ccaccgcatt cggtttgggt gtggcggtaa ttttcgtact cgggctgtcc gtccctgtca
    17521 accaactcgt ttactcgctg ctcaaagacg gcgcgattgt cgaaggcgtg gatttgacct
    17581 ttttgaaatt catcaccttc atcggcgtga ttgccgcttt ggtgcagatt ttggaaatgt
    17641 tcttggataa attcgtcccc gccctctata acgcattggg catctacctg ccgctgatta
    17701 ccgtgaactg cgcgattttc ggcgccgtat cgtttatggc gcaacgcgaa tacaacttcg
    17761 gcgagtccgt tgtatacggc ttcggcgcgg gcttgggctg gatgttggcg attgtcgctt
    17821 tggcgggcat taccgaaaaa atgaaatatt cggacgctcc caaaggcctc aaaggcttgg
    17881 gcattacctt tatcgccgcc ggcctgatgg cgatggcgtt tatgtcgttc tctggcatcc
    17941 agttataaga aagggatcgg tatggagatt attttaggta tcgtgatgtt taccgtcatc
    18001 gttttggttt tggcactgat gattctgttt gccaaatcca aactggtcag cgaaggcgac
    18061 atcaccatca aagtcaacgg cgaaaaagag ctgacgatgc ccgccggcgg caaactcttg
    18121 ggcgcgcttg ccaacgaagg catctttatc ccctccgcct gcggcggggg cggttcgtgc
    18181 ggacaatgcc gcgtcgtcgt aaaaagcggc ggcggcgaca tcctgccgac cgagctgtcc
    18241 cacatcagca aacgcgaagc acgcgaaggc tgccgtctgt cgtgccaagt caacgtcaaa
    18301 accgacatgg acatcgaagt ccccgaagaa gtgttcggcg tgaaaaaatg ggaatgcacc
    18361 gtcatctcca acgataacaa agccacgttc attaaagaac tcaagcttgc cattcccgaa
    18421 ggcgaagaag tccccttccg cgccggcggc tacattcaaa ttgaagcccc gccgcacacc
    18481 gttgcctaca aagacttcga cattcccgag gaataccacg aagactggga caaatacaat
    18541 ttgtggcaat acgtttccaa agtggacgag ccgattttgc gtgcctactc tatggcttcg
    18601 tatcctgaag aaaaaggcat cattatgctg aacgtgcgta tcgccacgcc gcccccgcgc
    18661 gtacctgatg cgcctccggg acaaatgtcg tcctacatct ggtcgctcaa acccggcgac
    18721 aaagttacga tttccggccc gttcggcgaa tttttcgcca aagacaccga tgccgaaatg
    18781 gtatttatcg gcggcggtgc gggtatggct ccgatgcgtt cccacatttt cgaccagttg
    18841 aaacgtttga actccaaacg taagattacc ttctggtacg gcgcacgttc caaacgcgaa
    18901 atgttctatg tcgaagactt cgaccaactc gcagcagaat tcccgaactt cacatggcac
    18961 gtcgccctgt ccgacccatt gcctgaagac aactgggacg gctacacagg cttcattcac
    19021 aacgtggttt acgaaaacca tctgaaaaac cacgaagcgc cggaagactg cgaattttat
    19081 atgtgtggtc ctccgatcat gaaccagtcc gtcatcaaaa tgcttaaaga cttaggcgtg
    19141 gaagacgaaa acatcctctt ggacgatttc ggcggttaaa tattcggctt caaacacaaa
    19201 aggaagaaac ctcggtttct tcctttttct atccgatgcc gcccgaaccc ccttcagacg
    19261 gcatcggcat agaaacatat cggccgtttt gcgatataat tcaaaccatt tgccaaaacc
    19321 gtcaaacact atgccgtctg aaacacgcct gccgaacttt atccgcgtct tgatatttgc
    19381 cctgggtttc atcttcctga acgcctgttc ggaacaaacc gcgcaaaccg ttaccctgca
    19441 aggcgaaacg atgggcacga cctataccgt caaatacctt tcaaataatc gggacaaact
    19501 cccctcacct gccgaaatac aaaagcgcat cgatgacgcg cttaaagaag tcaaccggca
    19561 gatgtccacc tatcagcccg actccgaaat cagccggttc aaccaacaca cagccggcaa
    19621 gcccctccgc atttcaagcg acttcgcaca cgttactgcc gaagccgtcc gcctgaaccg
    19681 cctgacacac ggcgcgctgg acgtaaccgt cggccccttg gtcaaccttt ggggattcgg
    19741 ccccgacaaa tccgttaccc gtgaaccgtc gccggaacaa atcaaacaag cagcatctta
    19801 tacgggcata gacaaaatca ttttgaaaca aggcaaagat tacgcttcct tgagcaaaac
    19861 ccaccccaag gcctatttgg atttatcttc gattgccaaa ggcttcggcg ttgataaagt
    19921 tgcgggcgaa ctggaaaaat acggcattca aaattatctg gtcgaaatcg gcggcgagtt
    19981 gcacggcaaa ggcaaaaacg cgcgcggcga accttggcgc atcggcatcg aacagcccaa
    20041 catcgtccaa ggcggcaata cgcagattat cgtcccgctg aacaaccgtt cgcttgccac
    20101 ttccggcgat taccgtattt tccacgtcga taaaagcggc aaacgcctct cccatatcat
    20161 taatccgaac aacaaacgac ccatcagcca caacctcgcc tccatcagcg tggtcgcaga
    20221 cagtgcgatg acggcggacg gcttgtccac aggattattc gtattgggcg aaaccgaagc
    20281 cttaaagctg gcagagcgcg aaaaactcgc tgttttcctg attgtcaggg atacaggcgg
    20341 ctaccgcacc gccatgtctt ccgaatttga aaaactgctc cgctaaacat acaaaccgat
    20401 taggaaacac catgaaaacc ctgctcctca ccttcggcat cttcctgacc gtcatcatcg
    20461 gtatggcggt cggctatatc ttctccaaac gcaccatcaa aggcagttgc ggcggcatta
    20521 ccgccttggg tatgaaaaaa atgtgcgact gcgacacgcc ttgcgacacc ctgcaaaaga
    20581 aactggatga agaaaaacag gcaggcggca tacgggttga ccgttaacac cgaaacaaaa
    20641 tgccgtctga agcccgacag gcgggttcag acggcattgc cgctttactt cgatcggttt
    20701 atacgcaaaa cgcgcttcag gaaataaatc agattattcc ccagacgcct tcaggcttcc
    20761 ttctgtgcca ccgtaaccat agccgggcgc aacacgcggt cggacagcgt ataacccttc
    20821 ttcatcacac ccaccacggt attgggttcc tgttcgcttg ccaccgcctg catcgcctga
    20881 tggatattcg gatcgagctt gtcgcccgct ttagggttga tttccttgat ttgcgtagca
    20941 tcaaatgctt tctgcaactc gttcaaagtc atctgcacgc ccattttcag cgcatcgaaa
    21001 ttgccgctct gatccaaaag cgccatttcc agataatcct tgaccggcag catttccacg
    21061 gcaaacttct gtccggcgaa cttgtgcgta tcggcaattt cctgctgatg gcggcggcgc
    21121 aggttttgct cgtttgccaa agcgcgcagc tgttcgtctt tcaactgcgc ttccagctcg
    21181 gcaatccgcg cctgcaaatc ctcataagcc ggctcggcgg cagcctgttc ctgcacaccg
    21241 tccgcatttc ctactgtctc gacggtttcc accgcctcca cattttcaac cgcttcttca
    21301 ctgttttgct gctgtgtctg ttcgctcata tcatatccct taaaacaaat tggaaatcaa
    21361 aatccggtta ttacccgcgc aagataagga cattttcaag aaacttcaag caaaggcagg
    21421 aaatttcaca tccgccgccg aataccctac cgcaaaaacc atataaacgg taagataccg
    21481 ccgaccttat gcttttcaga cggcatccat tatgaaaaaa aaccatttga acacaaccgg
    21541 tttcgacctc tggcacacca tccgcgaaga aaccgcggcc gctgccgccg ccgaaccgat
    21601 gctggcaagt tttttgcacc aaaccgtgtt gcgccacgag tccctcggct ccgtccttgc
    21661 ctaccacctt tccagcaaac tcggcagccc gattatggac gtgcgcgcgc tgtttgaaat
    21721 ctaccagcag gcattgggca gcgatgagca aatcagcaaa tgcgtcgagg cagacttaaa
    21781 agccatctac gaacgcgatc ccgcctgcga cgaatattcg ctgccgcttt tatatttcaa
    21841 aggttttcac gcgattcagg cacaccgcat caaccactgg ctatacttaa acggacgtaa
    21901 aacgctggcc tatttcctgc aaaaccggat gtctgaagta tttggggtgg acatccatcc
    21961 cgccgcgcat ttcggacacg gactgatgct tgaccacgcc accggctttg ttgccggcga
    22021 aaccgccgtg ttgggcaaca atatttcgat tttgcacggc gtaacgctcg gcggttcggg
    22081 caaagaaggc ggcgaccgcc accccaaaat cggcgacggc gtgatgatcg gcgcaaacgc
    22141 ctcgatattg ggcaatatcc gcatcggcag caatgccaaa atcggcgcgg gcagcgtcgt
    22201 ggtttcagac gtgccgcctt ccatcacggt tgtcggcgta cccgccaaac ccgtggcgcg
    22261 gtcgctcaaa accccgtcgg cggatatgga tcaaaatatc cagtttgccg aaatcgactt
    22321 tatgatttga atatccgcac caatgccgtc tgaaacgcca aacgggcttc agacggcatt
    22381 gcccgcttca agcttgcgcc ttgatatgcc gccgataccg acacttatca gtcggacaaa
    22441 atacctttta ttcgatacac ccgattccca cgcaaccg
//
LOCUS       DAWFIE010000025        22468 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWFIE010000025 DAWFIE010000000
VERSION     DAWFIE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22468
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.025"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(164..286)
                     /locus_tag="WGQ71_000906"
                     /pseudo
     CDS             complement(164..286)
                     /locus_tag="WGQ71_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(614..1060)
                     /locus_tag="WGQ71_000907"
                     /pseudo
     CDS             complement(614..1060)
                     /locus_tag="WGQ71_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(1103..1333)
                     /locus_tag="WGQ71_000908"
                     /pseudo
     CDS             complement(1103..1333)
                     /locus_tag="WGQ71_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(1362..1691)
                     /locus_tag="WGQ71_000909"
     CDS             complement(1362..1691)
                     /locus_tag="WGQ71_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0706949.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLEDGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(1956..2090)
                     /locus_tag="WGQ71_000910"
                     /pseudo
     CDS             complement(1956..2090)
                     /locus_tag="WGQ71_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
     gene            2212..3165
                     /locus_tag="WGQ71_000911"
     CDS             2212..3165
                     /locus_tag="WGQ71_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0706950.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPDVRKGYVSFEDAVRTADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALVAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(3240..5297)
                     /gene="metG"
                     /locus_tag="WGQ71_000912"
     CDS             complement(3240..5297)
                     /gene="metG"
                     /locus_tag="WGQ71_000912"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ0706951.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAHDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CGRIGIDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLTAQSEAIAECYESREYAKALRD
                     IMALADAVNEYVDANKPWELAKQEGQDARLHEVCSELINAFTMLTAYLAPVLPKVAEN
                     AAKFLNLEAITWANTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAVPAA
                     EESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATAEGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(5413..7251)
                     /gene="glmS"
                     /locus_tag="WGQ71_000913"
     CDS             complement(5413..7251)
                     /gene="glmS"
                     /locus_tag="WGQ71_000913"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ0706952.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGNIK
                     RVRRVGRVQLMEDAAREKHLNGSLGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEDLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLSYHTALARGTDVDKPRNLAKSVTVE"
     gene            7534..8052
                     /locus_tag="WGQ71_000914"
     CDS             7534..8052
                     /locus_tag="WGQ71_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706953.1"
                     /translation="MKQMLLAVGVAAVLAGCGKDAGSYEGYWREKSDKKEGMIVVKKE
                     KGNYFLNKINVVTGKEESMLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARKALPSNQTYQQHQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
     gene            complement(8486..9811)
                     /locus_tag="WGQ71_000915"
     CDS             complement(8486..9811)
                     /locus_tag="WGQ71_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ0706954.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPA
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMQQNPQRLAEVLGQNPSYIFF
                     RELTGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGETAGKMKEPGYVWQLLPNGMKPEYRP"
     gene            10022..10862
                     /locus_tag="WGQ71_000916"
                     /pseudo
     CDS             10022..10862
                     /locus_tag="WGQ71_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
     gene            10859..12025
                     /locus_tag="WGQ71_000917"
     CDS             10859..12025
                     /locus_tag="WGQ71_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ0706955.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGETQSANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            12289..13554
                     /gene="efeB"
                     /locus_tag="WGQ71_000918"
     CDS             12289..13554
                     /gene="efeB"
                     /locus_tag="WGQ71_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ0706956.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPVHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(13626..13955)
                     /locus_tag="WGQ71_000919"
     CDS             complement(13626..13955)
                     /locus_tag="WGQ71_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ0706957.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(14030..15400)
                     /gene="glmU"
                     /locus_tag="WGQ71_000920"
     CDS             complement(14030..15400)
                     /gene="glmU"
                     /locus_tag="WGQ71_000920"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ0706958.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLKTLLEAAGDKVGLLTDVPTDPTGLGRIIRDGNGSVTAIVEEKDASTA
                     QKAVKETNTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVSDGIKVRPVRVRA
                     SHLAAGVNNKLQLAELERIFQTGQAQELLKAGVTLHDPARFDLRGRLKHGQDVVIDAN
                     CIFEGEIELGDNVEIGASCVIKNAKIGANTKIAPFSHLEDCEVGENNRIGPYARLRPQ
                     ARLADDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(15455..15727)
                     /locus_tag="WGQ71_000921"
     CDS             complement(15455..15727)
                     /locus_tag="WGQ71_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ0706959.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPDQTDDSEQGVRGQNWQKPKFQNR"
     gene            complement(15720..16433)
                     /locus_tag="WGQ71_000922"
     CDS             complement(15720..16433)
                     /locus_tag="WGQ71_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ0706960.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFHLINRAMTRYMAHRLKLSES
                     AASDLRQEYWHRYGATLAGLQIHHPEIDIADFLRESHPINAILTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVWFGAKSHALPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTTPENTKECYA"
     gene            16492..17493
                     /locus_tag="WGQ71_000923"
     CDS             16492..17493
                     /locus_tag="WGQ71_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0706961.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            17587..18435
                     /locus_tag="WGQ71_000924"
     CDS             17587..18435
                     /locus_tag="WGQ71_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0706962.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            18461..18946
                     /locus_tag="WGQ71_000925"
     CDS             18461..18946
                     /locus_tag="WGQ71_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ0706963.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLAAVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(19048..20616)
                     /gene="msrAB"
                     /locus_tag="WGQ71_000926"
     CDS             complement(19048..20616)
                     /gene="msrAB"
                     /locus_tag="WGQ71_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ0706964.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            20761..22026
                     /gene="ftsY"
                     /locus_tag="WGQ71_000927"
     CDS             20761..22026
                     /gene="ftsY"
                     /locus_tag="WGQ71_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ0706965.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESETDQVIENIKEDAE
                     SLAESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAVAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         4779 a         5589 c         6721 g         5379 t
ORIGIN      
        1 gcccgctgcc gcctaaaagg cagcccggat gcctgattat cgggtatccg ggcaggatta
       61 aggggatatt tgggtaaaat tagtggatat ttgaaacgaa aacagccgaa aacctgtgtt
      121 tgggtttcgg ctgtcgggag ggaaaggaat tttgcaaagg tctctttcct ttgattttag
      181 aggaggatgc cacgccggca tcactgttgc cgccgggcca tttgccgtga ttcaggtaat
      241 actcggtgac ggctgatttt tgaccttcag ccaaaagaat ggcttcgtca ttcccgcgca
      301 ggcgggaatc caggtctgtc ggtgcagaaa cttatcggga aaacggtttc ttgagatttt
      361 gcgttctgga ttcccacttt cgtgggaatg acgggattaa agtttcaaaa tttattctaa
      421 aataactgaa actcaacgaa ctagattccc gcttttgcgg gaatgccgaa ttttaggttt
      481 cttattttgg ttttctgtcc ttgtgggaat gatgaaattt taagttttaa gaatttatcg
      541 gaaaaaacag aaaccgctcc gccgtcattc ccacgaaagt gggaatccag aactcaggac
      601 tggagaaacc tttttacccg ataagtttcc gcaccgacaa acctagattc ccgcctgcgc
      661 gggaatgacg aagtttttct gccatttgcc gtgattcagg taatactcgg taacggctga
      721 ttttttgaaa gtgtttgaaa tcggcgcgtg gtgtttttgt gcaaacggca gatgaagcgt
      781 cgcggcaggt tgacggcagg tgcttggtgt cgatcttgcc gttggcgtcg gtggcggcgg
      841 taacgccgtc gtcggtggcg gctttggcgc gcgtaaccgg ctgtccgcag aaccatttta
      901 ccgaaccgtc ttgacgcttg gcccacaggg agagtttttt gccttggatt tctttgttta
      961 cgccgcttga aagcattgtg gcggtaacga cgccgtttgc gactgtaact tccttaacat
     1021 attttccttt gattgttgaa gaagatgcca cgccggcggc actattgtcg gatggccatt
     1081 cgccgtgatt cgggtaatac tcgggtgttt ttgtgcaaac ggcagatgac gcatcgcggc
     1141 aggttgacgg caggtgcttg gtgtcgattt tgttaccgct gtcggcggtg acttcgtcgg
     1201 tgccggcttt ggctttggcg tcggcgcgct caaccggctg tccgcagaac cattttaccg
     1261 aaccgtcttg acgcttggcc cacagggaga gttttttgtc tttgatttcg ttgtttacgt
     1321 tgcttgaagc cattatgtca gacggtattg cccgggcagc tttattcgta cactttcagc
     1381 agctcgactt caaatatcaa agtggcgtgc gggggaatca cgccgccggc gccgtgtgcg
     1441 ccgtagccca tttccgaagg gatggtcagc ttgcgtttgc cgccctcctt cattccgccg
     1501 aagccttcgt cccagccttt gatgacttgt ccgacgccga gcgtgatggt cagcggctgg
     1561 cggcggtcga ggctggagtc gaatttggtg ccgtcttcca gccaacctgt gtaatgcacg
     1621 gtaatctctt tgcctttaac tgcttctttt ccgaagcctt cctgcaaatc ttcaataatc
     1681 aggccgccca tatttgtcct ttcattgctt gttggtcaaa acggcaaggg taacataccg
     1741 tccggcgaag tcaaatgccg ctcaaacgtc agccgcaccg gtgcagttga aacggctgtg
     1801 tttgttggac tgttttattt ttttcgtaaa ggttccatgc tttttcatgg aaatatagtg
     1861 gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacaaatag tacggcaagg
     1921 cgaggcaacg ccgtactggt ttttgttaat ccactataga aaacgacggt gttgattagg
     1981 ggcgcgacca gcgcgaccgc ccccgatacg cctatactgc ccgtcagtac ataggttaca
     2041 ctgaaggcgg tgctgaaatg cagtgcggca aaagtcaggg ttttaagcat catcctctcc
     2101 cgaattggac attgacggag gaatgataaa ggttatcata aggttgcgcg gtttaaattt
     2161 gctatttgtt gttagtgtaa ataaatcatt tttcaaatgg ggatagaaat tatgaatcat
     2221 aaaaagatcg ttgttttgga tgcggatact ttgcccggcc gggtttttca ttttgatttt
     2281 ccgcacgagc ttgcggttta cggtacgaca ggtgcggatg aaacggcaga acgggtgcgc
     2341 gatgcacata ttgtcattac taacaaagtg atgatttctg ccgatattat tgcggctaat
     2401 ccgcagttgg agctgattgc cgtcagtgcg accggtgtga acaatgtcga tattggggcg
     2461 gcgaaggcgg cgggcgttgc ggtatgcaat gtccgcgcct acggaaacga atcggttgcg
     2521 gaacacgcct ttatgctgat gattgcgttg atgcggaatt tgcccgccta tcagcgtgat
     2581 gttgcggcag gattatggga gaagtcgccg tttttctgcc attacggcgc gccaatcagg
     2641 gatttgaacg gcaaaacgct ggcggttttc ggacgcggca atatcggacg gacgctggcc
     2701 ggatacgcgc aggcgttcgg gatgggggtg gtgtttgccg aacacaaaca cgcgcccgac
     2761 gtgcgaaaag gctatgtttc ctttgaagat gcggtacgga ctgccgatgt gttgtcgctg
     2821 cactgtccgc tgaacgcaca aactgaaaat atgataggcg aaaatgagtt gcggcagatg
     2881 aagcccggcg cggttttaat caattgcggg cgcggtgggc tggtggatga aaatgcgctg
     2941 gttgccgcac tcaaatacgg gcagattggc ggggcaggtg tcgatgtttt gacggaggaa
     3001 ccgcctaggg gcggcaatcc cttgctgaat gcacgacttc ccaatctgat tgttacgccg
     3061 cataccgcgt gggcaagtcg tgaagcgttg gacaggctgt tcgatatatt gttggcgaac
     3121 attcacgcct ttgtgaaagg cgaggcgcaa aaccgcgtgg tttgatttta ttgggattgc
     3181 ggaaaacaat gccgtctgaa cgcctcaagg gttcagacgg cattttttga gatttctgct
     3241 taaccgactt tgtcgcccgg ttgcgcgcct gcatccacgt caagcagctt cagtttgccc
     3301 tctgctgtgg cggcactcaa aatcatgcct tcggatacgc cgaattttgc cattttgcgc
     3361 ggggcgaagt tggcaacggc gatgaccatt cttccgttta actcggcagg gttgggataa
     3421 gacgcggcga tgccggagaa gatgatgcgt ttttcaaaac cgaaatcgag gtcgaatttc
     3481 aggagtttgg tgctgccttc gacggcttcg cagttcaata ctttagcgac gcgcatgtcg
     3541 attttcataa agtcgtcaaa gctggcctgt tcggcaactt tttcgtattt gctctcttcg
     3601 gcggcaggca cggctgcggc ggcgatgctt tgtttgttgg cttcgattaa atcgtccact
     3661 tgtttttgct ccactcgttg cattaaatgt tcgtatttgt tgatggcgtg ctcgcccaag
     3721 gtttcgcgtg tatttgccca agtgatggct tccaaattca ggaatttggc ggcgttttcg
     3781 gccactttcg gcaacacggg ggcgaggtag gcggtcaaca tggtaaaggc gttgatgagc
     3841 tcgctgcata cttcgtgcag gcgtgcgtct tggccttctt gtttcgccag ttcccacggc
     3901 ttgttggcat caacgtattc gttcactgcg tctgccaatg ccatgatgtc gcgcagggct
     3961 ttggcgtatt cgcggctttc gtagcattcg gcaatggctt cgctttgtgc ggtcagtttt
     4021 gccagcaatg cgctgtcggc aacgtctttc agacggcctt caaagcgttt ggcgataaaa
     4081 cctgaggcgc gggcggcgat gttgacgtat ttgccgacga ggtcgctgtt gacgcgcgag
     4141 ataaagtctt gcaggttcaa atcgatgtct tcgattttgc tgttgagttt ggcggcgatg
     4201 tagtagcgca tccactcggg gtttaggcct tgttccagat aggatttggc ggtaataaac
     4261 gtgccgcgcg attttgacat tttttgtccg tcgacggtta ggaagccgtg tgcgtacacg
     4321 ccggtcgggg cgcggtggcc ggagaaatgc agcatagcgg gccagaacag ggcgtggaaa
     4381 tagaggatgt ctttgccgat gaagtggtac atctcggttt ggctgtcggc tttgaagtat
     4441 tcgtcgaaat cgatgccgat gcggccgcac aggtttttaa acgatgccat gtagccgacg
     4501 ggcgcgtcca gccagacgta gaagtatttg cccggcgcgt cggggatttc aaaaccgaaa
     4561 tacggcgcgt cgcgggaaat gtcccagtcg gacagcgtgg tttcttcgcc ttcgcccagc
     4621 cattctttca ttttgttgag ggcttcggct tgcagatggg gcttgccgtc gtgcgggttg
     4681 ttgccggaag tccatgcttt gaggaagtcg gcgcattcgc ccagtttgaa gaagaagtgt
     4741 tcggattcgc gcaattcggg tttcgcgccg gaaacggcgg aatacgggtt gatcagttcg
     4801 gtcggggaat aggtcgtgcc gcagacttcg cagttgtcgc cgtattggtc gtgggcgtgg
     4861 catttggggc attcaccttt gacgaaacgg tcgggcagga acatttgttt ttcggggtcg
     4921 aaaagctgct cgatgacgcg gctctcaatc ttgccgttgg ctttcagcgc gcggtaaatg
     4981 tcttgggaaa actgtttgtt ttcaggggaa tgggtgctgt aataattgtc gtaaccgatg
     5041 aaaaagccgg tgaagtcggc gaggtgttct tcgcgcactt tggcaatcat gtcttcgggc
     5101 gcaatgcctt gtttttgcgc ggcaagcatc acgggcgtgc cgtgggtgtc gtcggcgcag
     5161 cagtagtggc actcgtggcc gcgcagtttt tgaaagcgca cccaaacgtc ggtttggatg
     5221 tgttcgacca tgtggccgag gtggatgctg ccgttggcat agggcagggc ggaggtaacg
     5281 aggattttgc gtgtcatatt gtgctttgca aacaatgggt aaaggcggat tataccgcaa
     5341 atcaaacggg gaaatgccgt ctgaagcctg aaaaatcggg cttcagacgg catttttgcc
     5401 aaccggcggg atttattcga cggttacgga tttcgccaga ttgcgcggct tgtctacatc
     5461 cgtgccgcgc gcgagggcgg tgtggtagga aaggagctgc acggggatgg tatgcacgac
     5521 gggggacagt tcgccgacat ggcgcggcgc acggataacg tgcacgcctt cggttgcatt
     5581 aaaattgctg tcgaggtcgg caaagacgaa aagttcgccg ccgcgtgcgc cgacttcctg
     5641 catattggct ttgactttgt ccaacaggct gtcgttgggc gcgatgacga cgacgggcat
     5701 gttttcgtcc accaaggcaa gcggcccgtg cttcagttcg ccggcaggat aggcttcggc
     5761 gtggatgtag gtgatttcct tcagctttaa cgcgccttcg agggcaatcg ggtaatggat
     5821 gccgcgccct aaaaacagcg cgctggtttt cttggcaaac tgttgcgccc aggcggcaat
     5881 ttgaggttcg aggtttaaag cgtgctgcac gctgccggga agctggcgga gttcttcggt
     5941 gtaacgtgct tcgtcttctt cggaaaccaa accgcgcact ttcgccagcg ttaccgccaa
     6001 accgaacagc gcaaccagct gcgtggtaaa tgctttggtt gaggcgacgc cgatttccgc
     6061 accggcacgg gtataaagca cgaggctgct ttcgcgcggc agggcggatt ccatcacgtt
     6121 gcagatggag aggctgtggc ggtgtcccaa ggattttgcg tatttcaacg cctccatcgt
     6181 gtccagcgtt tcgccggatt gggaaatggt aatgaccagt tggtccggat cggcaatcac
     6241 gctgcggtat cggtattcgc tggcgatttc gacatcggcc gggattttgg cgatggattc
     6301 caaccaatat ttggcggtca gcgcggcgta ataggacgtg ccgcaggcaa ggattttgac
     6361 gctgcggatg ctttcaaaca cgcttttggc gtttttgccg aagttttcgg ggatgaagcc
     6421 gccgtcgagg aaaacctccg ccgtgtctgc aatcgcgcgc ggctgctcat ggatttcctt
     6481 ttgcataaag tggctgtacg gtcccagttc caaagaggcg agcgagagtt cggatacctt
     6541 gactttgcgt tcggcaggca ggccgttttt atcggtcagc cttttgatgc cgtctgaagc
     6601 cagcagcgcg atgtcgccgt cttcgaggta cgccacgcgg cgcgtaaagg cgatgacggc
     6661 ggatacgtcc gaagcgataa aggtttcatc gtcgcccaaa gcgaccaaaa gcgggcagcc
     6721 catacgcgcc acgactaatt catcaggctt gtcttgggca ataaccgcga tggcgtatgc
     6781 gccgtggaaa cgtttgaccg cttcctgcac ggcttcaaac agcctgccgc cgttttgcgc
     6841 gtattcgtga ttgatgctgt gggcgatgac ttcggtatcc gtttgcgatt caaaacggta
     6901 tcccaaatct tccaaacgtt tgcgttcgct ttcaaagttt tcgatgatgc cgttgtgcac
     6961 gaccgcaatc ataccgccgc tgatgtgcgg gtgggcgttc ggctcggtaa cgccgccgtg
     7021 tgtcgcccag cgcgtatgcc cgatgcccaa actgccgttc aaatgctttt cacgcgccgc
     7081 atcttccatt aactgcacgc gcccgacacg gcgcacgcgc ttgatgttgc cgtcggtgtt
     7141 gacggcaatg cctgatgaat cataaccccg gtattcgagg cgtttgagac cgtcggtcag
     7201 aaaatcgacg acattgtgat gggcgcggat agcgccgacg ataccgcaca taactgttcc
     7261 ttagtatccg gttgaaaaaa aaacaggcgc ggacggctcc cgtgccgcac cttccgcttc
     7321 ggattataaa ccgcctcccg cgccggaaaa cagcaaaatg ccgtctgaag gcttgggctt
     7381 gctcaaaaaa aggagggatt tccccgcctg accgggatgg gcgttcagac ggcattgcct
     7441 gctgctggtt tatagttttt gtaaatcaat attgacaagc tgaaaaaaaa aaacaatata
     7501 ctcgcttggt cttaatgtta aaggagtatg gaaatgaaac aaatgctttt ggccgtcggc
     7561 gtggcggcgg tgttggcggg ctgcggcaag gatgccggta gttacgaggg ttattggcgc
     7621 gaaaaatcgg acaaaaaaga gggtatgatt gtcgtcaaaa aagaaaaagg caattacttc
     7681 cttaataaaa tcaacgtggt tacaggtaag gaagaatcta tgcttttgtc tgaaaaagac
     7741 ggcgcgcttt cgataaacac ggggataggg gaaatcccga tcaaactttc cgacgacggg
     7801 aaagagctgt atgtcgaacg caggcagtat gtcaaaaccg atgcggcgat gaaggacaaa
     7861 atcatcgccc atcagaaaaa gtgcggacaa acggcacagg catacctcga cgcgcgaaag
     7921 gcgttgccgt caaaccagac gtatcagcag catcaggcgg cgatcgagca gttgaaacgg
     7981 cggtttgaag ccgagttcga cgaattggaa aaagaaatca aatgcaacgg caaaccgaca
     8041 ttgttgtttt agcgtaggac aaccgggagg atgccgccgt ccgaatcgga tgtgtggttt
     8101 tcgtaccggt acgggcgggc aggaatgtcc gctttttttg ttcggacgcg tttgaatatc
     8161 cgtccgattc cgtccgtttg caaagggtat ttttgttcgg gcggaaatta tagtggatta
     8221 acaaaaacca gtacagcgtt gcctcgcctt agctcaaaga gaacgattct ctaaggtgct
     8281 gaagcaccaa gtgaatcggt tccgtactat ttgtactgtc tgcggcttcg tcgccttgtc
     8341 ctgatttttg ttaatccact ataattccgt caaataagaa aggcgttttg tccgaccggc
     8401 cggcttgtgc ctgcggtatc gcaaaactcc gccttaatgc gcccgattgc ctagggacgg
     8461 gcttcagacg gcattgtttt ccggtttacg ggcggtattc gggcttcata ccgttgggca
     8521 gaagctgcca gacgtaaccc ggttctttca ttttaccagc cgtttcccct gcttcgtcgc
     8581 cgtatcccca aaaataatcc acgcgcaccg cgcctttaat cgcgctgccg gtatcctgcg
     8641 ccataatcag gcggttgagg gctttgcggg taaccggatg ggcggtggcg acaaataagg
     8701 gcgcacccaa ggtaatgtag tgccggtcga ctgcgccggc gtactcgccc atcagcggcg
     8761 tgcccagtgc gccgacaggg ccgtcattgc tgcttccggt aagctctcgg aaaaagatat
     8821 agctggggtt ttgccccaaa acttcggcga ggcgttgcgg gttttgctgc atataggctt
     8881 tgatgccctg catcgaggtc tgcccgagct tgaggtagcc tttgtccgcc atatagcgtc
     8941 cgatggaaac gtagggatgt tcgtttttgt cggcatagcc gatgcggatg tatttgccgg
     9001 acggggtttt cagacggccc gagccttgga tgtgcataaa aaaaagttcg acggggtctt
     9061 cggcgtaacc gagtatcggg gctttgccgt caagcgcgcc gccgttgatt tggttgcgcg
     9121 tgtggtaggg gaggaagcgg cttccttcaa acctgccttt gattgccgtt gtgcgcgcgg
     9181 tgatggggaa tcgggagagg tcggcggtgt gcgtgccgcc ggcattgtcg attgtgccgc
     9241 tgtttttccc cgtctgcctg atgcggacaa gggcttttcc gctccgcaaa ccggcaggca
     9301 gggggacgga gataaaatcg tcgggaatac cgtaaatcgg gaagcgggct tgtgccgtcc
     9361 gcctgtcgtc gcccttcagc accggctcgt aatagccggt aaccgtaccg gcaaggcttc
     9421 cgttgcctgc aacctgccac ggcgtgaaat agcgttcaaa aaactgtttt gcctgaaagg
     9481 aatggacggg ggtttgaaag gcttgggcgc acacatcctg ccagccttgg cggtttttca
     9541 aattggcgca gccgaggcgg aaggattgca ggcttttggc gaaatcctgc gccgcccagt
     9601 ggggcaggga caggtgcggc acaacggtat aaacggcccc gccgccgccg accgtcgttc
     9661 cggcggggtc ggggatgccg gccggccggt ccgggccgtt gatgacggat gtgtcgggtt
     9721 gcggaaaggt ttggatgctc ttgctttggc aggcggcgag gatggcggcg gcgatgccgc
     9781 acagggcggc gcggaacagg tattttttca taatggacaa tgttgccggc agtaataaga
     9841 aagatggttt cgggcggcgt tgcggcagcc gtggagaggg gattttaaca cagggcgcag
     9901 ctgcagcctg cggaactttc cgccgcgcgg tactgcagat aaaaataact tgcatttgta
     9961 tttacaagca atgaaaatat tccgataata tattattcat catccttgtt cgttcgcgtt
    10021 tatgctggtc gcttttttaa ttatgttgcg cgagggtatt gaagccgcgc tcattgtcgg
    10081 catcgttgcc ggttttctga aacagtccgg acattccaaa ctgatgccta aggtctggtt
    10141 cggggtcgtc cttgcttctt tgatgtgttt ggggctgggg tacggcatcc attcggcaac
    10201 gggcgagatt ccccagaagc agcaggagtt cgtcgtcggc attatcggtt tggttgctgt
    10261 cgcgatgctg acttaatatg attttatgga tgaaaaaggc ggcgcgttcg atgaagcggc
    10321 agcttcagga ttctgtgcag gcggctttga accgtggcag cggtcaagga tgggccttgg
    10381 tcggtatggc gtttcttgcc gtggcgcgcg aaggtctgga gagcgttttt ttcctgtttg
    10441 ccgtattcaa acagagccct acgtggcaga tgccggccgg tgcggtagcg ggggttttgg
    10501 ctgccgccgt gattggcgcg ttgatttatc agggcgggat gcgcctgaat ctggctaagt
    10561 tttttcgttg gacgggggcg tttctgattg tcgttgccgc cggcctgctt gccggctcgc
    10621 tgcgcgcgct gcatgaggca ggtatttgga acgcgcttca ggatattgtg ttcgactcat
    10681 caaaatattt gcacgaagac agcccgttgg gcgtgctgct cggcggattt ttcggctata
    10741 ccgaccatcc gacgcagggc gaggccttgg tttggctgct gtaccttatt cccgtcatga
    10801 cttggttttt gtgcggcagc aggccgtctg aaactttaac ccgtaaagag gagctgaaat
    10861 gagaaaattc aatttgaccg cattgtccgt gatgcttgcc ttaggtttga ccgcgtgcca
    10921 gccgccggag gcggagaaag ctgcgccggc agcgtccggt gagacccaat ccgccaacga
    10981 gggcggttcg gtcagtatcg ccgtcaacga caatgcctgc gaaccgatgg aactgaccgt
    11041 gccgagcgga caggttgtgt tcaatattaa aaacaacagc ggccgcaagc tcgaatggga
    11101 aatcctgaag ggcgtgatgg tggtggacga gcgcgaaaac atcgcccccg gactttccga
    11161 taaaatgacc gtcaccctgt tgccgggcga atacgaaatg acttgcggtc ttttgaccaa
    11221 tccgcgcggc aagctggtgg taaccgacag cggctttaaa gacaccgcca acgaagcgga
    11281 tttggaaaaa ctgtcccaac cgctcgccga ctataaagcc tatgttcaag gcgaagtcaa
    11341 agagctggtg gcgaaaacca aaacctttac cgaagccgtc aaagcaggcg acattgaaaa
    11401 ggcgaaatcc ctgtttgccg acacccgcgt ccattacgaa cgcatcgaac cgattgccga
    11461 gcttttcagc gaactcgacc ccgtcatcga tgcgcgtgaa gacgacttca aagacggcgc
    11521 gaaagatgcc ggatttaccg gctttcaccg tatcgaatac gccctttggg tggaaaaaga
    11581 cgtgtccggc gtgaaggaaa ttgcagcgaa actgatgacc gatgtcgaag ccctgcaaaa
    11641 agaaatcgac gcattggcgt ttcctccggg caaggtggtc ggcggcgcgt ccgaactgat
    11701 tgaagaagtg gcgggcagta aaatcagcgg cgaagaagac cggtacagcc acaccgattt
    11761 gagcgacttc caagccaatg tggacggatc gaaaaaaatc gtcgatttgt tccgtccgct
    11821 gatcgaggcc aaaaacaaag ccttgttgga aaaaaccgat accaacttca aacaggtcaa
    11881 cgaaattttg gcgaaatacc ggactaaaga cggttttgaa acctacgaca agctgggcga
    11941 agccgaccgc aaagcgttac aggcctctat taacgcgctt gccgaagacc ttgcccaact
    12001 tcgcggcata ctcggcttga aataagccgc aagcgttcag acggtatttg gcggcagata
    12061 ccgtctgaag ttttatagtg gattaacaaa aaccagtacg gcattgcctc gccttgcctt
    12121 gccgtactat ttatactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact
    12181 atatccgcca tatattgcag ggcaggattt caacctgccg ctatcggtta atggaaaaac
    12241 ggcgtgcagg gatacccatc ctgctgcacg gatattgaag gaaacaccat gagcaaaaac
    12301 caacccgcac aaccgaccag gcgcactctt tttaaaaccg cgatcgcagc cggagcagtc
    12361 ggcgcaatcg gaggttatct cggcggcaaa aaacagggcg aaaccgccga acgcaccgcc
    12421 gaaagccaac actcgcccca agcctatccc tgctacggcg aacatcaggc aggcatcgtt
    12481 acgccgcagc aggcgttttc gattatgtgc gccttcgacg taaccgcgca aagtgccaag
    12541 cagctggaaa acctgttccg cacgctgacc gcccgcatcg agtttctcac ccaaggcggc
    12601 gaataccaag acggcgacga caaacttcca cccgccggca gcggcatttt gggcaaagcc
    12661 ttcaaccccg acgggttgac cgttaccgtg ggggtgggca gcagcctgtt tgacggccgg
    12721 ttcggactca aagacaaaaa accggttcat ttgcaggaaa tgcgcgactt ctccaacgat
    12781 aagctgcaaa aaagctggtg cgacggcgat ttgagcctgc aaatctgcgc cttcaccccc
    12841 gaaacctgcc aagccgccct gcgcgacatc atcaaacaca ccgtccaaac cgccgttatc
    12901 cgttggagta tcgacgggtg gcagcctaaa tccgaacccg gcgcgatggc ggcgcgcaac
    12961 ctgttgggct tcagggacgg cacgggcaac cccaaagttt ccgaccccaa aactgccgac
    13021 gaggttttgt ggacgggtgt ggccgccaac agcctcgacg aaccggagtg ggcgaaaaac
    13081 ggcagctatc aggcagtccg ccttatccgc cactttgtcg agttttggga caggacgccg
    13141 cttcaagagc aaaccgacat tttcgggcgg cgcaaataca gcggtgcgcc gatggacggc
    13201 aaaaaagaag ccgaccaacc ggattttgcc aaagaccccg aggggaatac cacgcccaaa
    13261 gacagccata tacgcctggc gaatccgcgc gatcccgaat tcctcaaaaa acaccgcctc
    13321 ttccgccgcg cctacagcta ttcgcgcgga ctcgcctcaa gcggacagct tgatgtcggg
    13381 ctggtgttcg tctgctatca ggcaaacctt gccgacggat tcatcttcgt gcaaaacctc
    13441 ctcaacggcg aaccgctgga agaatacatc agccccttcg gcggcggcta tttcttcgtc
    13501 ttgcccggcg tggaaaaagg cggctttttg gggcaagggc tgctgggcgt ataaatccgc
    13561 catataaaaa acgccgtccg aaccttgcca aacgggttcg gacggcgttt cttgtttttg
    13621 ggcggtcagg cttttttgac gaattcggat tttaaattca tcgcgctgcc gtcgattttg
    13681 cagccgatgt tgtgatcgcc ttcctgcagg cgtatgcctt tgacttttgt accttgtttg
    13741 atcaccatcg agctgccttt taccttgagg tctttgatga ggatgacggt atcgccgttt
    13801 tgcagtgccg cgccgttggc atcgcgcact tgagccgcaa ggtcggcggc ggattcggtt
    13861 tcattccatt cgtgggcgca ttcggggcag atgtattgtc cgccgtcttc ataagtgtat
    13921 tcggaggcgc attgtgggca tgggggtaat gacatggttt gcagtcctta tcggatgttt
    13981 gttttggggt accgtctgaa acctgaaacc ggcttcagac ggcatagctt tattgtttgt
    14041 ctttttcggg acgcacccag ccttcgatga cggtttggcg ggcgcgggcg agggcgagtt
    14101 tgttgtcttc gatattgcgg gtaatcgtgc tgcctgcgcc tgtggttact ttgttgccca
    14161 gtgttacggg ggcaaccagt acgcagtttg aaccgatgcg cacttcgtcg ccgatgacgg
    14221 ttttgtgttt gtgcacgccg tcgtagttgg caataatcgt accggcgccg aagttggttt
    14281 tgctgccgac ttcggcatcg ccgatgtagg tgaggtggtt ggctttggtg cctttgccga
    14341 tggcggcgtt tttgatttcg acgaagttgc cgacgtgtac gtcgtctgca aggcgggctt
    14401 gcggacgcag gcgggcgtac gggccgattc ggttgttttc gccgacttcg cagtcttcga
    14461 ggtgggagaa gggggcgatt ttggtgttgg caccgatttt ggcgtttttg atgacgcagc
    14521 ttgcgccgat ttcgacgttg tcgccgagct cgatttcgcc ttcaaagata cagtttgcat
    14581 caatcacgac atcttgcccg tgtttcagac ggccccgcaa atcgaaacgt gccgggtcgt
    14641 gcagggtaac gcctgctttg agcaattctt gcgcctgtcc ggtttggaag atgcgttcga
    14701 gttcggcgag ctggagtttg ttgttcacgc cggcggcgag gtgagaggcg cgcactcgga
    14761 cggggcggac tttgatgccg tctgaaacgg ctttggcgat gaggtcggtc agatagtatt
    14821 cgccttgggc attgttgctg gaaaggctgt tcagccagtt ttccagtttg gcgttgggca
    14881 ggacgagaat gcctgtgttg gtttctttca cggctttttg ggcggtactt gcgtcttttt
    14941 cttcgacaat ggcggttacg ctgccgttgc cgtcgcggat gatgcgcccc aagcctgtcg
    15001 ggtcggtggg aacatcagtc aacagcccga ctttgtcgcc tgcggcttcg agcagggttt
    15061 tgagggtttc aacgtcaatt aaaggaacgt cgccgtacag caccagcgtg cggccttcgg
    15121 cggcaaggtg gggcagggcg gttttgacgg cgtggccggt gccgagctgt tcggtttgtt
    15181 cgacccaaac gacatcgcgt ttgacggtgt ctaaaacttg ctctttgccg tggccgacga
    15241 cgacgcagat gttttgcgga ttcagcgcgg cggcggtgtc gataacgcgc ccgagcatgg
    15301 gcttgccgcc gatgcggtgc agcactttgg gcattttgga atacatgcgc gtgcctttgc
    15361 cggcggcgag gataacgatg tttaaagtgt tttgcggcat gacggtttcc tgtgcaatgc
    15421 cgtctgaagc agcttcagac ggcatagggt gggtttatcg gttttgaaat ttgggctttt
    15481 gccagttctg tccgcgtacg ccttgttcgg aatcgtcggt ttggtcgggc agcacggcat
    15541 ggcgttcggg acggtattgg ttgtagttca tatttttcga gtagctgccg tcttggtaat
    15601 aaacgggcgt gccggcggga tatttttgac ggacggcggt cttgccgttg ccgtcttgat
    15661 aagtttccca cgcgcagccc gacaaaaggg cggcggcagt catcaggata aggaaggttt
    15721 tacgcataac attctttcgt attttcgggg gttgtgcgag tattgtaatg attttggcgg
    15781 tgttctgaca aagtttctgc ataccgagcc agttgcgcca tatcgcttac ggaagcatcg
    15841 ataaagggca gcgcgtggga ttttgcgccg aaccagacgg ttttcatgcc caatgctttt
    15901 gcctgatgca ggttgtccgc gctgtcgtcc accataatgc agcattcggg cggtacgtcc
    15961 aaaaggcggc agacattgag atacgcttgc ggattgggtt tgtacagcag tccgaaatca
    16021 tccgtgccga acagtgcgtc gaaacggttt tccaaaccga gtgcgccgac aacggcgcgg
    16081 acgtaaaacg acgggccgtt ggaaaaaact gccttgcgcc cttttaggcg gctcagggtg
    16141 ttttgtgttt caggcatgcc gtgcagccta gtcaggattg cattgatcgg atggctttcg
    16201 cgcaaaaaat cggcgatgtc gatttcggga tggtggattt gcagtccggc aagcgttgcg
    16261 ccgtagcggt gccaatattc ctgacgcagg tcggacgcgg cggattcgga gagtttgagg
    16321 cggtgtgcca tatagcgtgt catagcgcgg ttgatgaggt ggaagatgcc ggcatcggca
    16381 ttgtgcagcg tgttgtcgag gtcgaacagc cacacggtcg ggttttcttg catgttgaac
    16441 cgtgaaaatt tgttagaatg ttattttaca gcgaatagag gaggactcgg aatgaaacgg
    16501 aaaatttggc tgctgccgct gctggcggtt tcggcatacc tgcaggcgca gacggaagtc
    16561 aggctggcgg tgcataagtc gttcagcctg cccaaagggt tgattgcgcg cttcgagcgg
    16621 gcaaacgatg cgaaggtgtc gattattcag gcgggcggcg cgaacgaaat gctcaacaaa
    16681 ctgattttga gccgcgccaa cccgattgcc gacgcggtgt atggtttgga caacgccaat
    16741 atcggcaagg cgcgggaaat gggcattttg gcggcggcgc aacccgaatc cgcccccgtc
    16801 gcggtcgggc tgccttcggc tttggcggtc gattacggct atgtgtccat caattacgac
    16861 aaaaaatggt ttgaaggcaa aaagctgccc ctgccgcaaa ccctgcagga tttgacccgc
    16921 cccgaatata aaaacctatt ggtcgtgccg tcccccgcca cgtcgtcccc ggggctgggc
    16981 ttcctgatgg cgaacatcag cggtctgggc gaagaaggcg cgttcaaatg gtgggcacag
    17041 atgcggcaga acggcgtgaa ggtcgccaaa ggctggagcg aggcgtatta caccgacttt
    17101 tcgcacaacg gcggcgcgta tccgctggtg gtcggttatg ccgccagtcc ggcggcggaa
    17161 gtgtattttt ccaaaggcaa atacagcgag ccgccgacgg gcaacctgtt tttaaaaggc
    17221 ggcgtattcc gccaggtcga aggcgcggca gtcttgaagg gcgcgaaaca gccgaagctg
    17281 gcggcaaaac tggtgcaatg gctgcaaagt cgggaagtgc agcaggcggt tccgtccgaa
    17341 atgtgggttt accccgccgt caaaaacacg cgcctgcccg acgtgttccg cttcgcccaa
    17401 gccccgacgc acaccaccgc ccccgcgcag cgcgatattg atgcgaacca gcgcggatgg
    17461 gtttcccgtt ggattagaac ggttttgaaa taaaacaaac ataccgcccc gcagggcttc
    17521 atacggcatt tttacacctg tgccgattac gccgcacggg gcggatgttc gatcaagagg
    17581 aaaacaatgg acttcaaaca atttgatttt ttacacctga tcagtgtttc cggttgggag
    17641 catctggctg aaaaggcgtg ggcgttcggg ctgaaccttg ccgccgcgct gcttattttt
    17701 ttggtcggaa aatgggcggc gaaacgcatt gtcgctgtga tgagggcggc gatgacgcgc
    17761 gcgcaggtcg atgccacgct gattagtttt ttgtgtaatg ttgccaatat cggcttattg
    17821 attttggtga ttattgccgc attgggcaga ttgggcgttt ccacaacatc cgtaaccgcc
    17881 ttaatcggcg gcgcgggttt ggcggtggcg ttgtccctga aagaccagct gtccaatttt
    17941 gccgccggcg cgctgattat cctgttccgc ccgttcaaag tcggcgattt tatccgcgtc
    18001 ggcggttttg aaggatatgt ccgagagatt aaaatggtgc agacttcttt gcggacgacc
    18061 gacaacgaag aagtcgtgct gcccaacagc gtggtgatgg gcaacagcat cgtcaaccgt
    18121 tccaccctgc cgctgtgccg cgcccaagtg atagtcggcg tcgattacaa ctgcgatttg
    18181 aaagtggcga aagaggcggt gttgaaagcc gccgtcgaac accccttgag cgttcaaaac
    18241 gaagagcggc aggccgccgc ctacatcacc gccttgggcg acaatgccat cgaaatcaca
    18301 ttatgggctt gggcaaacga agcagaccgc tggacgctgc aatgcgactt gaacgaacaa
    18361 gtggtcgaaa acctccgcaa agtcaatatc aacatcccgt tcccgcaacg cgacatacac
    18421 atcatcaatt cttaaacgcc gtctgaaagg ggagtgggaa atggacgcgc tgcacaccat
    18481 cgcccgaaac ctgacgaaaa aacgtcaaac cgtaagctgt gccgaatcct gcacgggtgg
    18541 gctgcttgcc gccgtattca caagcgttgc aggcagttcg caatggttcg atcaaagttt
    18601 tgtaacatac agcaacaaag ccaaagaaga ccgcttgggc gtgttgcccg aaaccctgct
    18661 cgaacacggc gcggtcagcc gccaaaccgt ctatgagatg gcgcgcggcg cgaaagccgt
    18721 ggcgcaggcg gattacgccg tcggtatttc cggcatcgcc ggtccgggcg gcggcagcga
    18781 aagcaaaccc gtcggcacgg tttggttcgg gtttgccttt ccgggcggaa gttgcgaagc
    18841 aatgcgccgt tttgacggca accgcgaatc cgtccgcgcg caggcggtcg ccttcgcgtt
    18901 ggaacggttg gcggggctga ttgaaaacgg cggcgatgcc gtctaaacaa aatctccgtc
    18961 tggacaaaat ccccgccgga taaaaaatac cgtctgaaac gttgcaggtt tcagacggta
    19021 ttttgtcggg ttaggcggcg gtgcggctta tttcacttta ctcttcaacg cgccgtagcc
    19081 tgccgcgtcc atttgttcca gcgggatgaa tttcaagctc gcgccgttga tgcagtagcg
    19141 cagtccgcct ttgtcgcgcg ggccgtcggg gaagacgtgc cccaagtgtg agtcggcggc
    19201 gtggctgcgc acttcggtgc ggcgcatgtt gtagctgaaa tcatcgtgtt cggtaacgga
    19261 ttttgcatca atcgggcgcg tgaagctcgg ccagccgcag ccggaatcat atttgtcggc
    19321 ggagctgaac aaaggttcgc cgctgacaac gtccacataa atgccgggtt tgaacaaatg
    19381 gtcgtattcg tggctgaagg catattcggt cgcgctgttt tgggtaactt ggtattgctc
    19441 ttcggtcagg gtgcgtttga gttcggcgtc actcggtttt ttatacgttg ccgcgtcgaa
    19501 gcctttgcct tgcggggcgg tcttggtttt gcccggcagc ggttcgtcgg ctttgcggat
    19561 atcgatgtgg cagtagccgt tggggttttt aatcaggtag tcctgatggt attcctcggc
    19621 atcgtagaag tttttcagcg gctcgttttc aacaacgagg ggcagttggt atttttgctg
    19681 ctcgcgtttg agggcggcgg cgatgacggc tttttcggcg gggtcggtgt agtacacgcc
    19741 gctgcggtat tgcgtaccgg tgtcgttgcc ctgtttgttg aggctggtcg gatcaacgac
    19801 gcggaagaaa tattgcagga tgtcgtctag gctgagtttg tcggcatcgt aggtcacttt
    19861 aacggtttcg gcgtggcccg tatggcggta ggacacgtct tcatagctcg gatttttcgt
    19921 gttgccgttg gcgtagccgg ataccgcgtc aaccacgccg tcgatgcgtt ggaaataggc
    19981 ttccaagccc cagaagcagc cgccggcgag gtaaatggtg cgtgtgttca tgatttttga
    20041 atcctttttc tgagtgtcgg gtttgtagaa cgaatgtttc aagctgccca aatcggcatt
    20101 cgggtcgcgg attaacgcca acgcctgcgc ttcgttgatg ctgcctttga cgatgcgctg
    20161 cacgtcgccg tctttaccga ttaacgccca cgaggggtaa acgctgatat tcaggctttg
    20221 ggcgatcgtg ccgccgttgt cggttacgac gggcagcttg ggataattca aaccggcata
    20281 ccatttttgg aagtcgccgt cttttttctc gtgcaaaaag cccggagacg cgacggtaat
    20341 caggttggcg gagctgaatt ttgcatcttg cgcccatttt tcggtctgtc ccaattcgga
    20401 caaacacaaa ggacaccagc tcgcccaaaa tttaatcagc gtcggtttgt cttttttcaa
    20461 gtaaacactg gcggggcggt tgtccgcagt tttcaaagtg gataaagtgt gcggcacggt
    20521 cgcggctccg gcatcgacga ttttgggcga acaagcaccc agcgcaagca ggcagccgaa
    20581 cttggcgcaa agggaaaaga aagtacggtg tttcattttg agtttcctgt gtggacggtt
    20641 tgcatgatta gacgtttgag atgccgaaac cttacagctc ggattttcag acaaccttgc
    20701 cgcgtaaaat acgctacaat acgccctgtt tcaagtttct aaaattaaaa ggaaaattca
    20761 atgttcagct tcttccgtcg caagaaaaaa caggaaacgc cggctctcga ggaggctcaa
    20821 attcaggaaa ccgcagcaaa agcagagtcc gaaaccgatc aagtaattga aaatattaaa
    20881 gaagatgctg aatctttagc agaaagcgtc aaagggcagg tcgaatctgc cgttgaaacc
    20941 gtcagcggtg cggttgaaca ggtaaaggaa accgttgccg agatgctgtc tgaagcagag
    21001 gaagcggcgg aaaaagcagc ggaacaagtc gaagcggcaa aagaagccgt tgccgaaacc
    21061 gtcggcgagg ctgtcgggca agttcaagaa gccgttgcga caactgaaga acacaagctc
    21121 ggttgggcgg cgcgtttgaa acaaggtttg accaaatcgc gcgacaaaat ggcgaaatcg
    21181 ctggcgggcg tgttcggcgg cggacaaatc gacgaagatt tgtacgaaga gctggaaacc
    21241 gtgctgatta ccagcgacat gggcatggaa gccaccgaat acctgatgaa agacgtgcgc
    21301 gaccgcgtca gcctcaaagg gctgaaagac ggcaacgaat tgcgcggcgc gttgaaagaa
    21361 gccttgtacg acctgattaa gcctctggag aaacctttgg ttttgcccga aaccaaagag
    21421 ccgtttgtca tcatgcttgc cggcatcaac ggcgcgggca aaaccacgtc tatcggtaaa
    21481 ctcgccaaat atttccaagc gcagggcaaa tctgtgctgc ttgccgccgg cgacaccttc
    21541 cgcgccgccg cccgtgagca gcttcaagct tggggcgaac gcaacaacgt aaccgtgatt
    21601 tcgcaaacca cgggcgattc cgccgccgtg tgcttcgatg ccgtccaagc cgccaaagcg
    21661 cgcggcatcg acattgtgct ggccgacacc gccggccgcc tgcccacgca gcttcatttg
    21721 atggaagaaa tcaaaaaagt gaaacgcgtg ctgcaaaaag ccatgcccga cgcgccgcac
    21781 gaaatcatcg tcgtgcttga tgccaacatc gggcaaaacg ccgtcaacca agtcaaagcc
    21841 tttgacgacg cattagggct gaccggttta atcgtgacca aactcgacgg cacggcaaaa
    21901 ggcggcatcc tcgccgcgct tgcctccgac cgccccgtcc ccgtccgcta tatcggcgtg
    21961 ggcgaaggca tagacgattt gcgcccgttt gacgcgcgcg cgtttgtgga cgcactgctg
    22021 gattgagccg aaatgccgtc cgaaaacagc agaccgatgc cgtcattccc gcgcaggcgg
    22081 gaatctagac cttagaacaa cagcaatatt caaaggttag ctgaagcttt agagattcta
    22141 gattcccact ttcgtgggaa tgacgggatg taggttcgtg ggaatgacgt ggtgcaggtt
    22201 tccgtatgga tggattcgtc attcccgcgc aggcgggaat ctagaacgta aaatctaaag
    22261 aaaccgtgtt gtaacggcag accgatgccg tcattcccgc gtaggcggga atctaggcca
    22321 ttggacagcg gcaatattca aagattatct gaaagtccga gattctagat tcccactttc
    22381 gtgggaatga cgggatgtag gttcgtggga atgacgcggt gcaggtttcc gtgtggatgg
    22441 attcgtcatt cccgcgcagg cgggaatc
//
LOCUS       DAWFIE010000026        21724 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWFIE010000026 DAWFIE010000000
VERSION     DAWFIE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21724
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.026"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            60..611
                     /locus_tag="WGQ71_000928"
     CDS             60..611
                     /locus_tag="WGQ71_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706966.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
     gene            complement(874..1565)
                     /locus_tag="WGQ71_000929"
                     /pseudo
     CDS             complement(874..1565)
                     /locus_tag="WGQ71_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1871..2428)
                     /locus_tag="WGQ71_000930"
     CDS             complement(1871..2428)
                     /locus_tag="WGQ71_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706967.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(2673..2831)
                     /locus_tag="WGQ71_000931"
     CDS             complement(2673..2831)
                     /locus_tag="WGQ71_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706968.1"
                     /translation="MKPPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2828..3670)
                     /locus_tag="WGQ71_000932"
     CDS             complement(2828..3670)
                     /locus_tag="WGQ71_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0706969.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3909..4829
                     /gene="pyrB"
                     /locus_tag="WGQ71_000933"
     CDS             3909..4829
                     /gene="pyrB"
                     /locus_tag="WGQ71_000933"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ0706970.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQCLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4839..5297
                     /locus_tag="WGQ71_000934"
     CDS             4839..5297
                     /locus_tag="WGQ71_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0706971.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            5362..6027
                     /locus_tag="WGQ71_000935"
     CDS             5362..6027
                     /locus_tag="WGQ71_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706972.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(6090..7307)
                     /locus_tag="WGQ71_000936"
     CDS             complement(6090..7307)
                     /locus_tag="WGQ71_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0706973.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            7449..7994
                     /gene="def"
                     /locus_tag="WGQ71_000937"
     CDS             7449..7994
                     /gene="def"
                     /locus_tag="WGQ71_000937"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ0706974.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            8081..9007
                     /locus_tag="WGQ71_000938"
     CDS             8081..9007
                     /locus_tag="WGQ71_000938"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ0706975.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            9086..10345
                     /gene="rsmB"
                     /locus_tag="WGQ71_000939"
     CDS             9086..10345
                     /gene="rsmB"
                     /locus_tag="WGQ71_000939"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ0706976.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            10311..10910
                     /locus_tag="WGQ71_000940"
     CDS             10311..10910
                     /locus_tag="WGQ71_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ0706977.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10910..13030
                     /locus_tag="WGQ71_000941"
     CDS             10910..13030
                     /locus_tag="WGQ71_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ0706978.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGLPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVKTYA"
     gene            13023..14300
                     /locus_tag="WGQ71_000942"
     CDS             13023..14300
                     /locus_tag="WGQ71_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ0706979.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QVRPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKHGAAKIETGPVFDKLGNSAAIQEMNREVGAAVKRASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            14391..15578
                     /gene="dprA"
                     /locus_tag="WGQ71_000943"
     CDS             14391..15578
                     /gene="dprA"
                     /locus_tag="WGQ71_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ0706980.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLRRFGSASNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRASARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRTLSGKGIPVVSGMASGIDT
                     AAHQGALQAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDSPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEA
                     AAGGTAHGGILDKMGFDPVHPDVLAEQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            15604..16065
                     /locus_tag="WGQ71_000944"
     CDS             15604..16065
                     /locus_tag="WGQ71_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ0706981.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            16137..18443
                     /gene="topA"
                     /locus_tag="WGQ71_000945"
     CDS             16137..18443
                     /gene="topA"
                     /locus_tag="WGQ71_000945"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ0706982.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIAGGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(18516..19211)
                     /locus_tag="WGQ71_000946"
     CDS             complement(18516..19211)
                     /locus_tag="WGQ71_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706983.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFVPEMN
                     RILSDFKTTRGFK"
     gene            complement(19231..19782)
                     /locus_tag="WGQ71_000947"
     CDS             complement(19231..19782)
                     /locus_tag="WGQ71_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706984.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHFHIQNGAVFFYSYRDV
                     TIVFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIIIYRPNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19901..20278
                     /locus_tag="WGQ71_000948"
                     /pseudo
     CDS             19901..20278
                     /locus_tag="WGQ71_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            20367..20936
                     /gene="rsmD"
                     /locus_tag="WGQ71_000949"
     CDS             20367..20936
                     /gene="rsmD"
                     /locus_tag="WGQ71_000949"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ0706985.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVFSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PNIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            21059..21310
                     /locus_tag="WGQ71_000950"
     CDS             21059..21310
                     /locus_tag="WGQ71_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ0706986.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDQEHPETHDELMAKYEKIIQFK"
     gene            21407..21490
                     /locus_tag="WGQ71_000951"
     tRNA            21407..21490
                     /locus_tag="WGQ71_000951"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21441..21443,aa:Tyr,seq:gta)
     gene            21521..21594
                     /locus_tag="WGQ71_000952"
     tRNA            21521..21594
                     /locus_tag="WGQ71_000952"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21553..21555,aa:Gly,seq:tcc)
     gene            21604..21678
                     /locus_tag="WGQ71_000953"
     tRNA            21604..21678
                     /locus_tag="WGQ71_000953"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21636..21638,aa:Thr,seq:ggt)
BASE COUNT         5357 a         5487 c         6004 g         4876 t
ORIGIN      
        1 tcctagttat ctaggacagc cccttgtttt ttgttcggcg gcttgcgtgg tcgggtaaaa
       61 tgaaagtttt gaacggttgg tcggacagga agatgtggcg ggttttgagt gctttgccga
      121 taggcgtggt gttttttgat ttgatctacg gttttgtgtt gaatgtgttg cagggtttgg
      181 atttgcagcg tgccgtgccg gattcggaag gcgtgttggc ggttacgccc gatattgcat
      241 tcaacagttt gcagattgtc gccaacggcg gtatggcggc ggtggtctgt ttcgggttgg
      301 cggttgtgtt tttgctcaac cgttcggtgc ggcggcggca ggtgttggaa atcggggtgt
      361 tccggatgtt ggggctggtg gcggtattgg cgttcagtgc gccgtcggtg tgggagtggg
      421 cgaacgcgct gccgctgctg ctgaagggcg cggacgtggt caatacgggg aatgcgcgtt
      481 atgtgctgac ggctttgtgt atgccctttc cggcggtgtc gtgcgtcatc gggctggtgg
      541 ggcggttcag gcttcagacg gcatcgggca gggcggcaaa gtcagggggt gcgggcaagg
      601 cggacggata ggacgcattt ttcagcgggt gcgtcgagaa gcagccgatg tgtttggcag
      661 ccgcagcttg gggggtagtg ctaatggcgg tttctttgct tttatagtgg attaacaaaa
      721 accagtacgg cgttgcctcg ccttagccca aagagaacga ttctctaagg tgctgaagca
      781 ccaagtgaat cggttccgta ctatccgtac tgtttgcggc ttcgtcgcct tgtcctgatt
      841 actatataaa ataaatgggc aaaaatcggt ttattatcgt ttttgccgca tttggatttg
      901 ttctaccgta aaacgtgttt gacgaacggg attcttatta aaaaacatct gatttctaac
      961 aaaatcagta ttttttggca cgatggctaa aatttttcct tccatttcgc catcacgtgt
     1021 tttccatgcg ctcaagaatt gtgatttgct cattgagacg tgccccagcg atggatcagc
     1081 cagcaaaaca gtttctccgt taataccgtt caataccgaa aaatggttgt ttttacggta
     1141 ttttaaatac acaattacag gaatttttag ttgtaccaac tgttcaaatg gcaaagcata
     1201 accttgtgct tcaaaaccca gttcgggcat tatgcgttgc atatcatcaa aagaagcacg
     1261 catttgggtt ttatccattt tgtctaagat ttccgcttca gaataatgtc tgccataaaa
     1321 attattcagt aacgtggcaa tcgaagccgc gccgcaagaa aaatccaaat cttgttttac
     1381 tatgccggaa tctcgccgtg ctttccaact ccgtacatgg atgttttggt aagaagcggg
     1441 gggtcaacaa acataggcca agcaaaaact atatttgggg cgaaaccaat caaagccgca
     1501 taatttatca atttataaag attttttatc ataatatgta tacgcggaat aaacgcatga
     1561 aacataaaaa aataaaatca tatgcaattt tattgcatga ataaatatga ataaaataga
     1621 taatgatggg agtaaatacg ccatgtattt tggaagttta aatttattaa taataaaata
     1681 atttatcgta gcgcaaataa atcccaaaat tggaacgatt agaaaaaaaa ttaatacacc
     1741 catcatcatg caaactctat attaataaat agctaactaa tgctattgtg gaaattagtt
     1801 agctaactaa aagttattaa tgattatttt cgagaattga ctgcattatt agcagcattg
     1861 gcaccaaaac ctagtgcatg aatacccggt ctccatgcca aattcccagc caatccgcct
     1921 ccggcagcag cagccagccc tgttttgccg ctactcctgt tgccgcaccg attcctgccg
     1981 cagtagccgc gccttgcgca gttcctaatt taccatgatt atacaaatta gcaccatgat
     2041 actcccatgc acctaatgca ccgccaaaag cagcggctgc aataatggga acaaattcac
     2101 cttgtgtttc tttcatttca gcctgtgata attgaattgc tttcacattt tggctgtcaa
     2161 aaacttggct gtctaaattt tgcgccatta caggtgtaat catcatagcc attacagttg
     2221 caattttcgt tgcgctggtt tgcacataaa taggattagc aaattcgctt tgattgcgtt
     2281 cagtgttgat gtagctaata ctgctttcta gtttgaattt acccttgtca gtcaataatt
     2341 cttccaaact taaaggtaag tcagcataag caatttggct ggcaacagtc aaaataaaat
     2401 ctattagaca tttgtgtttt tgcatcattt cgtttgattt tctagttttt gagaatgata
     2461 caaagttttt ttacaaagta aagagtcact ctgaaaaaca ttttttcatt ataaatcaaa
     2521 atattgatag aataaatagc gagcatcgat tcacggtgta ctttagtgca aagcgtacaa
     2581 cgcgagcctg aaccaccagc cgcaacagga aaagaaagcc gatagagtgc aatgcttgcc
     2641 aacgtgcaag cgagcttgca agaacgcttg gctcaacgag agcaggcaag acagaaagca
     2701 gaaagcagga taggagcggt aacgcaaagg tctcgggctt tgatttcgcc gtaaaccctg
     2761 ctgccgcctt gtccggaaag ggtgcaggcg gcgagtgccg acagggtgca gatggggagg
     2821 gggggtttca tttggggtcg caacggaagt ggtatgcgca gatttcaaaa ccgtttttga
     2881 aatacaggcg gtgcgcgtcg gcacggtcgt ggttgacgtg gacgttgagg tggattttgg
     2941 ttacccctgt ttccgcgccg attttgcgga cttcttccaa aaggcgcgag gcgtagcctt
     3001 tgcggcggct ttgcggcagg gtaacgatgt catcgatgtg gatgtggcgg ccgctggcga
     3061 gggtgcaggc ttcgcggaag ccgcagacgg cgacggcatt gtgtttgcct tcttcaaaaa
     3121 tacccagcag gcggtagcct tgggggcgtt ggactttgtt gatctgttcg gtaaagcggt
     3181 tgatgtcggt cagggcggaa cgcaaaacgc tcaaggctgc aaaggcggtg gcggtgtcgt
     3241 ccgcgccgat ttcgcgcaaa acgtaggatg cgcccgaggc ggtctgttcc tgtgctttct
     3301 cggcggcgtg tttttcttcg attgcctgtg ccagcatgac gtgttcgtcg gcagggttgt
     3361 tttgtccgcc ctgttcgcgt tcttcgagca gggctttgca gtcgatgacg cgcaggtcgt
     3421 tgtcggcggc aaagtccatc aggaagcgga acatttgggg gttgtcggtt tccagttttt
     3481 tatcgacggc gacgcagcgg atgttgtcca ccagcatggg gcgcacccat ttcgaatagg
     3541 aaagcctgtg gtctttggtg aacgaggaca gaatgccgca caggcgttcc gcccagtcgc
     3601 tgggacggaa aatcttgccg gaactcgttg tgccgtggat gacgacttcg taggggttgc
     3661 agactaacat ggcggcttcc tgaaaagaaa tgtctggcgc gattatacct tatgcttatg
     3721 cgggcgtgtt tggatatgcc gtctgaaaag tacgggattc gtgcggtaaa actttgcggc
     3781 ggcaaatgtg cgataatacg cgccgtattg ccgcttttgc gaagctgttc cgcaaacata
     3841 cgggcggcgt ggacgacgta taaccggata cccgcctgac gcgggttttt tacggaaggg
     3901 gggcaaaaat gcctaatccg ctttacagac agcatatcat ctccatttcg gatttgtcgc
     3961 gcgaacagtt ggaatgcctg cttcagacgg cattgaagct gaaggcgcat ccgcgcggcg
     4021 acctgttgga aggcaaactt atcggttcgt gctttttcga gccgtccacg cgcacgaggc
     4081 tgtcgtttga aacggcggtg cagtgtttgg gcggcaaggt catcggcttt tcggacggcg
     4141 cgaataccag tgcgaaaaaa ggcgagacgc ttgccgatac cgcccgcatc atttccggct
     4201 acaccgacgc tatcatccaa cgccacccca aagacggcgc ggcgcgcgtg gcagcggagt
     4261 tttcgcgcgt ccccgttatc aacgccggcg acggcacgaa ccagcacccc agtcagacct
     4321 tgttggatct ggtaacgatt tatgaaacgc aggggcgttt ggacaagctc aaaatcgcga
     4381 tggcgggcga cttgaaatac gggcgcaccg tgcattcgct ttgtcaggcg ttgaaacgct
     4441 ggggctgcga atttgccttc gtctcgccgc ccagcctcgc gatgcccgac tacattaccg
     4501 aagagttgga cgaagccggc tgccgatacc gtatcctcgg cagtttggaa gaagcggcgg
     4561 aatgggcgga tatcctgtat atgacccgcg tccagcgcga acgtttcgac gaacaggaat
     4621 ttgccaaaat ccaaggcaaa ttcaacctcg aagcgtctat gctcgcccgc gccaaaccga
     4681 acctgcgcgt gctgcacccc ctgccgcgcg tggacgaaat ccatcccgat gtcgatgcca
     4741 cgccgcacgc ctattatttc gagcaggcga ccaacggcgt ttatgcgcgt atggcgatat
     4801 tgtcgctggt gttgaacgaa gaagtgtgag gaaccgatat ggaaaccccg aaactcagtg
     4861 tcgaagccat tgaaaaaggt acggttatcg accatattcc cgccggcagg gggctgacca
     4921 tcctgcgcca gttcaaactt ttgcactacg gcaacgcagt aaccgtgggc ttcaacctgc
     4981 ccagcaaaac ccaaggcagc aaagacatca tcaaaatcaa aggcgtgtgc ttggacgaca
     5041 aagccgccga ccgccttgcc ctgtttgcac ccgaagcagt ggtcaacacc atcgacaatt
     5101 tcaaggttgt gcagaaacgg catttgaacc tgcccgacga aatcgccgaa gtgttccgct
     5161 gtccgaacac gaattgcgcc ggccacggcg agccggtcaa aagccggttt tatgttaaaa
     5221 aacacaacgg gcagacgcgg ctgaaatgcc attactgcga aaaaacctac agccgggatt
     5281 cggtggcgga agcctgacgg attcccttaa accgagtggg cggcatttcg tctgccgcct
     5341 gttttgccaa tctgaaatgg aatgatgatg cacgcttctg tccaaagccg tttcgcaccg
     5401 atactttatg ttttgatttt ctttgccggt tttttgaccg cgcaaatctg gttcaatcag
     5461 aaagcctata ctgaagagct gcctccgctt ctgtccgcat tgtccgctgt cgcgctggtg
     5521 tggctggcgt gggcgttcgt gtcggcgcgt tcaaaggcta aggcggaaaa gttctaccgc
     5581 gaaaaaatga tacagaacga aagcatacat cccgtcctgc acgcttcttt gcaacacttg
     5641 gaacacaagc cgcaaatgct cgccctgctg gtcaaaaacc acggcaaagg gatggcggaa
     5701 caggtcaggt tcaaggcgga agtgctgccc gacgacgaag acgcgcgcac gattgccgcc
     5761 gagttggcaa aaatggatat gtttgcattg gggacggacg cggtcgcctc gggcgaaacc
     5821 tatggacgcg tgttcgccga tattttcgag ttgtcggcgg ctttggaagg gcgcgcgttc
     5881 aaaggaatgt tgaaactgac ggcggaatat aaaaacatct tcggcgatgc ctgccgttcg
     5941 gaaacggcgt tggagttggg cgcgctcaat caggcgttgc aggagatttc aaaaacatcg
     6001 gaaaagtcca aacggatatt ttattgaaga tggaaaaatg ccgtctgaaa cggaaggtgt
     6061 ttcagacggc atttttgtcg gatgattaat tattcggagc ggttgaagcc aaacttcacg
     6121 cggctgcggc cctgatccgg tatattgtcc aaatcgcgtc ccggattggc ggcggtgtcg
     6181 cctacggaaa tatcggagat gttttccaaa atgatggcgg acgacaggtg ttcggaggtg
     6241 cggtaaacca ttgccaagcc cacttcttcg gcaggagtgg aaatcagctc gacggtatcc
     6301 ctgcttttga aattgttgga gaggtcgacc tgcatcgttt tcttgcgttt gtagaggctc
     6361 aaaaccgtgc ctttgtccaa accgtccgcc tcgcctttgt cgatggtgat ggtttgaaac
     6421 tggccggcaa tccttgtgcc ttcaaacacg gaaacgattt tagcctgaac cgggcgggac
     6481 ggttcgtgcg gcatcatgtt gaagcggtcg gtgtcttccg gcattttcat caggtagtcg
     6541 ccctgctgta tttcggaaat ggcggtttcg accaccagcg gctgtatcga aggggtgcgc
     6601 agcggggtaa tcaaaggatg ggtgcgggta tggtattcgt tgtctttcgg ccgttctcca
     6661 gcctgtttcg agcgttgttc gaggacagag tcggtatagt cgagggagcg cacgatgccg
     6721 ctgaatgcga cttcctgccc gaggaattta cccgtatccg gatcggtgat gtttttattg
     6781 attcggtagg tcaggtagcg gcccggctct ttcaggcctt tggtgtaaac cctggtgcct
     6841 ttggtgtaca gcagcctgcc ttccgggccc gagagcaggc gcggcgcggc agcggtttct
     6901 ttgcgggaaa cgatttgcgg gtgccgcata aagatgcggt agaagttgac atcgatggcg
     6961 ggaataccgt atccggacac ttccttatcc ggactcattt tgacgacggg gatgccgtct
     7021 gtctgttcca agccgaggcg cggttcgccg tcaacgtggc gcaacaccaa tacctggtcc
     7081 ggataaatca ggtcgggatt gtggatttga tcccggttcg cgtcccacag gcggccccat
     7141 tgccacgggc tgtacaggta tttgcccgaa atgccccaca gggtgtcgcc ctgtttgacc
     7201 gtgtagcgtt ccggcgcgtt cgggcgcacc tccaaatttg ccgccaaagt ttgtgttgag
     7261 aatgccatac ctgccgcgca gagcagggtt ataatacgac gttgcataac cgttcccctt
     7321 atctgataaa tttcggtttg tcttgcttga ttgggttgga aaaagcggcg gcagcccctc
     7381 gggatgtgcc gcgtgataaa aaatgttccg cattttaaca tcgaattatc cgcaccatca
     7441 cggtaattat gaaaaacagg cggcgtatcc gccgaaggaa agagaaaatt atggctttat
     7501 tgaatatctt gcaatatccc gacgagcgtc tgcacacggt ggcaaagcct gtcgaacaag
     7561 tcgacgagcg catccggaag ctgattgccg atatgtttga aacgatgtac gaatcgcgcg
     7621 gcatcgggct ggcggcgacg caggtcgatg tgcacgagcg cgtggtcgtg atggatttga
     7681 ccgaagaccg cagcgaaccg cgcgtgttca tcaaccccgt catcgttgaa aaagacggcg
     7741 aaaccactta cgaagagggc tgcctgtccg tgccgggcat ttacgacacc gtaacccgcg
     7801 ccgaacgcgt caaggtcgag gctttgaacg aaaaaggcga aaagttcacg ctggaggcgg
     7861 acggcttgct ggcgatttgc gtgcagcacg agttggacca cctgatgggc atcgtgtttg
     7921 tcgaacgcct gtcgcagctc aagcagggac gcatcaaaac caagctgaaa aaacgtcaga
     7981 aacatacgat ttgacccttt tgccgtgccg tctgaacgct gcaaagtttt cagacggcac
     8041 ggtcttgtcc gacaacctta cgcatacgca ggaatatgct atgaaagtca tctttgccgg
     8101 cacgcccgat tttgccgccg ccgccttaaa agccgttgcc gccgccggtt ttgaaatccc
     8161 gctggtgctg acccagcccg accgtccgaa agggcgcggt atgcaactga ccgccccgcc
     8221 cgtcaaacaa gccgcgctgg aactcggttt gcgcgtggca cagcccgaaa aactgcgtaa
     8281 caacgccgaa gccctgcaaa tgctcaaaga ggtcgaggca gacgtaatgg tggttgccgc
     8341 ctacggtttg attctgccgc aggaagtgtt ggatacgccg aaacacggct gcctcaacat
     8401 ccacgcttcg ctgttacccc gttggcgtgg cgcggcgccg attcaacgcg cgattgaagc
     8461 cggcgatgcc gagacaggcg tgtgtattat gcagatggac atcggtttgg acaccggcga
     8521 tgtggtcagc gaacaccgct acgccatcca accgaccgat accgccaacg aagtccacga
     8581 cgcgctgatg gaaatcggtg cggcggcggt tgttgccgat ttgcaacagc ttcaaagcaa
     8641 aggccgtctg aacgcggtca aacagcccga agaaggtgtt acttacgccc aaaaactaag
     8701 caaagaagag gcgcgtatcg attggagcga aagcgcggcg gttatcgaac gcaaaatccg
     8761 cgccttcaac cccgtgcctg ccgcgtgggt tgagtatcag ggcaagccga tgaaaatccg
     8821 gcgggcggaa gtggtggcgc aacaaggcgc ggcaggcgaa gtgttgtcct gttcggcgga
     8881 cggtttggtc gttgcctgcg gcgaaaacgc gctgaagatt accgaattgc agcctgccgg
     8941 cggcaggcgt atgaatatcg cggcgtttgc agcaggacgg catatcgaag cagggacgaa
     9001 gctgtaaatc ccttcagacg gcattccgat ccgcaaatgg gaatgccgtc tgaagtcatc
     9061 agttgaagaa agcgaatcac ataatatgag tatggcactt gcccaaaaac ttgccgccga
     9121 cagcattgcg gcggttgccg aaggacgcaa ccttcaggac gtgttggcgc aaatccgcac
     9181 cgcgcatccc gaccttacgg cgcaggaaaa cggcgcgttg caggacatcg cctacggttg
     9241 ccagcgttat ttgggcagtt tgaaacatat gctcgcgcag atgctgaaaa aaccgattga
     9301 caatccgcag ctcgaaagcc tgcttttggc ggcgttgtac caactgcatt acacgcgcaa
     9361 cgcgccgcac gccgtggtca atgaggcggt ggaaagcata gccaaaatcg ggcgcgggca
     9421 gtaccgttcg tttgccaacg cggttttgcg ccgctttttg cgcgaacgcg acaagctcgc
     9481 ggcttcctgt aaaaaagacg atgtggcgaa acacaacctg ccgctgtggt gggtggctta
     9541 cctgaaaaac cattatccga aacactggca caacatcgcc gccgcgctgc aatcccatcc
     9601 gccgatgacg ttgcgcgtca accgccggca cggcaacgcc gaaagctatt tggaaaaact
     9661 ggctgcggaa ggcattgcgg ctaaggcgtt ggacgaatat gcggttacgt tggaagaagc
     9721 cgtgccggta aaccgcctgc ccggtttttc agacggcatc gtttcggtac aggacttcgg
     9781 cgcgcagcag gcggcgtatc tccttaaccc gaaagacggc gaacggattt tggacgcgtg
     9841 cgccgcgccg ggcggcaaga cggggcatat cttggaactg gcggattgcc gcgttaccgc
     9901 cttagacatt gatgcaggcc gtctgaagcg tgtggaggac aatatcgccc gtttgggctt
     9961 tcagacggca tcggcggcgt gtgccgatgc gcgggatttg gcggcgtggt atgatggaaa
    10021 gcctttcgac gcaatccttg ccgacgtgcc atgcaccgct tccggcgtgg cgcggcgcaa
    10081 tcccgacgtg aaatggctgc gccgtccgac cgacgcgctc aaaaccgccc gccagcagga
    10141 agccctgctg gacgcacttt ggcagacgct gaaacagggc ggcagaatgc tgcttgccac
    10201 ctgttccgtg ttcgtcgaag aaaacgacgg gcaattgcaa aaattcctca accgccatgc
    10261 cgatgccgaa ccgatcgaat cgcgggtgct tttaccgaac aaacaccaag atggctttta
    10321 ttacgcgctt attcaaaagc attaaacaat ggcttgtgct gctgccgatg ctttccgttt
    10381 tgccggacgc ggcggcggag gggatagatg tgagccgcgc cgaagcgagg ataaccgacg
    10441 gcgggcagct ttccatcagc agccgcttcc aaaccgagct gcccgaccag ctccaacagg
    10501 cgttgcgccg gggcgtgccg ctcaacttta ccttaagctg gcagctttcc gccccgataa
    10561 tcgcttctta tcggtttaaa ttggggcaac tgattggcga tgacgacaat attgactaca
    10621 aactgagttt ccatccgctg accaaccgct accgcgttac cgtcggcgcg ttttcgacag
    10681 actacgacac cttggatgcg gcattgcgcg cgaccggcgc ggttgccaac tggaaagtcc
    10741 tgaacaaagg cgcgctgtct ggtgcggaag caggggaaac caaggcggaa atccgcctga
    10801 cgctgtccac ttcaaaactg cccaagcctt ttcaaatcaa tgcattgact tctcaaaact
    10861 ggcatttgga ttcgggttgg aaacctctaa acatcatcgg gaacaaataa tgcgccgttt
    10921 tctaccgatc gcagccatat gcgccgtcgt cctgttgtac ggactgacgg cggcaaccgg
    10981 cagcaccagt tcgctggcgg attatttctg gtggatagtt gcgttcagcg caatgctgct
    11041 gctggtgttg tccgccgttt tggcacgtta tgtcatattg ctgttgaaag acaggcgcga
    11101 cggcgtattc ggttcgcaga ttgccaaacg cctttctggg atgtttacgc tggttgccgt
    11161 actgcccggc gtgtttctgt tcggcgtttc cgcacagttt atcaacggca cgattaattc
    11221 gtggttcggc aacgataccc acgaggcgct tgaacgcagc ctcaatttga gcaagtccgc
    11281 attgaatctg gcggcagaca acgcccttgg caacgccatc cccgtgcaga tagacctcat
    11341 cggcgcggct tccctgcccg gggatatggg cagggtgctg gaacattacg ccggcagcgg
    11401 ttttgcccag cttgccctgt acaatgccgc aagcggcaaa atcgaaaaaa gcatcaaccc
    11461 gcacaagctc gatcagccgt ttccaggtaa ggcgcgttgg gaaaaaatcc aacgggcggg
    11521 ttcggtcagg gatttggaaa gcataggcgg cgtattgtac gcgcagggct ggctgtcggc
    11581 gggtacgcac aacgggcgcg attacgcctt gtttttccgt cagccggttc ccaaaggcgt
    11641 ggcagaggat gccgtcttaa tcgaaaaggc aagggcgaaa tatgctgagt tgagttacag
    11701 caaaaaaggt ttgcagacct ttttcctggc aaccctgctg attgcctcgc tgctgtcgat
    11761 ttttcttgca ctggtcatgg cactgtattt cgcccgccgt ttcgtcgaac ccgtcctatc
    11821 gcttgccgag ggggcgaagg cggtggcgca aggcgatttc agccaaaccc gtcctgtttt
    11881 acgcaatgac gagttcgggc gtttgaccaa gttgttcaac cacatgaccg agcagctttc
    11941 catcgccaaa gaagcagacg agcgcaaccg ccggcgcgag gaagccgcca gacattatct
    12001 tgaatgcgtg ttggaggggc tgaccacggg cgtggtggtg tttgacgaac aaggctgtct
    12061 gaaaaccttc aacaaagcgg cggaacagat tttggggttg ccgcttaccc ccctgtgggg
    12121 cagcagccgg cacggttggc acggcgtttc ggcgcagcag tccctgcttg ccgaagtgtt
    12181 tgccgccatc ggcgcggcgg caggtacgga caaaccggtc catgtgaaat atgccgcgcc
    12241 ggacgatgcc aaaatcctgc tgggcaaggc aaccgtcctg cccgaagaca acggcaacgg
    12301 cgtggtaatg gtgattgacg acatcaccgt tttgatacac gcgcaaaaag aagccgcgtg
    12361 gggcgaagtg gccaaacggc tggcacacga aatccgcaat ccgctcacgc ccatccagct
    12421 ttccgccgaa cggctggcgt ggaaattggg cgggaagctg gacgagcagg acgcgcaaat
    12481 cctgacgcgt tcgaccgaca ccatcgtcaa acaggtggcg gcattgaagg aaatggtcga
    12541 agcattccgc aattatgcgc gttccccttc gctcaaattg gaaaatcagg atttgaacgc
    12601 cttaatcggc gatgtgttgg cattgtatga agccggtccg tgccggtttg cggcggagct
    12661 tgccggcgaa ccgctgatgg tggcggcgga tacgaccgcc atgcggcagg tgctgcacaa
    12721 tattttcaaa aatgccgccg aagcggcgga agaagccgat gtacccgaag tcagggtaaa
    12781 atcggaagcg gggcaggacg gtcggattgt cctgacggtt tgcgacaacg gcaaagggtt
    12841 cggcagggaa atgctgcaca acgccttcga gccgtatgta acggacaaac cggcgggaac
    12901 gggattgggt ctgcctgtgg tgaaaaaaat cattgaagaa cacggtggcc gcatcagcct
    12961 gagcaatcag gatgcgggtg gggcgtgtgt cagaatcatc ttgccaaaaa cggtaaaaac
    13021 ttatgcgtag cagcgatatt ttaattgtag acgacgaaat cggcatccgc gacctgctgt
    13081 cggaaatcct gcaggacgaa ggttattcgg tcgcattggc ggaaaacgcc gaagaggcgc
    13141 gcaagctgcg ccatcaggta cgcccggcga tggtgttgct ggatatttgg atgcccgatt
    13201 gcgacggcat cacccttttg aaggagtggg cgaaaaacgg gcagctcaat atgccggtgg
    13261 tgatgatgag cgggcatgcc agcatcgata ccgccgtaga agcgaccaaa atcggcgcgc
    13321 tcgacttttt ggaaaaaccg atttccctgc aaaagctgct gtctgccgtc gaaaacgcgt
    13381 tgaaacacgg tgcggcgaaa atcgaaacgg gaccggtgtt cgacaagctg ggcaacagtg
    13441 cggcgattca ggaaatgaac cgtgaggtag gggctgcggt gaaacgtgcc tctcccgtac
    13501 ttttgacggg cgaggcgggt tcgccgtttg aaacggtggc acgctatttc cataaaaacg
    13561 gtacgccgtg ggtcagcccg gcaagggtcg aatatctgat cgatatgccg atggaactgt
    13621 tgcagaaggc ggagggcggc gttttgtatg tcggcgacat cgcccagtac agccgcaaca
    13681 tccaagccgg tattgccttt attgtcggaa aggcggaaca ccgccgcgtc agggtggtcg
    13741 catcgggcag cagggcggca ggttcagacg gtattgcctg cgaggaaaag ctggcggaac
    13801 tgctgtcgga gtcggtcgtc cgcattccgc cgctgcgtat gcagcatgaa gatattccct
    13861 tcctgataca ggggattgcc tgcaatgtgg cggaaagtca aaagattgcg cccgccgcat
    13921 tcagtgaaga ggcacttgcc gcattgaccc gttacgactg gccgggaaat ttcgaccaac
    13981 tgcaaagcgt cgttgcaacg ctgttgttgg aggcggacgg acaggaaatc ggcgcggcgg
    14041 cggtttcttc ccttttgggg cagaatgtgc ctgccgaggg agcggaagat atggtgggcg
    14101 ggtttaattt caacctgccc ctgcgcgaat tgagggagga ggtggagcgg cgttatttcg
    14161 agtaccacat cgcccaagaa ggtcagaata tgagcaaagt ggcgcagaaa gtcggtttgg
    14221 aacgcacgca cctttaccgc aaactcaaac agctcggcat cggcgtttcg cgccgggcgg
    14281 gggaaaaaac cgaagaatag gcccggacgg ccggttttcc ggctgcgggc ttttgttttc
    14341 agacggcatt tggtgcaaat gccgtctgaa atcgtaaggg gacggatttt atgacagagg
    14401 acgaacgttt cgcgtggctg caattggcgt ttacgcccta tatcggcgcg gaaagcttcc
    14461 tgctgctgct gcgccgtttc ggcagcgcgt caaacgccct gtccgcaccg gcggaacagg
    14521 tcgccccggc ggtgcggcac aagcaggcgg ccgaggcttg gcgcaatgcg gaaaaacgcg
    14581 cctcggcgcg gcaggcggca gaagcggcgt tggaatggga aatgcgggac ggatgccgtc
    14641 tgatgctgct tcaggatgaa gatttccccg aaatgctgac gcaggggctg accgcgccgc
    14701 cggttttgtt tttgcgcggc aacgtgcggc tgctgcacaa gccttccgcc gccatcgtcg
    14761 gcagccgcca tgccacgccg caggcgatgc ggattgccaa agatttcggt aggacgttgt
    14821 ctgggaaagg cattcccgtt gtgtcgggta tggcttcggg catcgatacc gccgcccatc
    14881 agggtgcgtt gcaggcagaa ggcggcacca tcgccgtgtg ggggacgggc atagaccgca
    14941 tttatccgcc gtccaataaa aaccttgcct atgaaatcgc cgaaaaaggg ctgattgtca
    15001 gcgagttccc catcggcacg cgcccgtatg ccggcaactt tccgcgccgc aaccgcctga
    15061 ttgccgcgct gtcgcaggtt acgctggtgg ttgaagctgc gttggaatcc ggttcgctga
    15121 ttaccgccgg cctggcggca gaaatggggc gcgaagtgat ggcggtaccc ggttcgatag
    15181 acagtccaca cagcaaaggc tgccacaaac tgattaaaga cggcgcaaaa ttggtggaat
    15241 gcctggacga catcctgaac gaatgcccgg ggctattgca aaatacgggt gcttcatcat
    15301 attctataaa taaggatacg cccgacacgg gacgccgcgc cgttcagacg gcatacgccc
    15361 cgccgcctgc cgcaaaaatg ccgtctgaag cggcggcagg cggcacagcc cacggcggca
    15421 tactggataa aatgggtttc gaccccgtcc atcccgacgt gcttgccgag cagcttgcta
    15481 tgcctgccgc agatttgtat gccgcactgt tggaattgga attggacggc agcgttgccg
    15541 caatgcccgg cggcagatac cagcgtatcc gaacttgaac gcactttata ttaaggaaca
    15601 cgaatgaccg aagtcatcgc ctacctcatc gaacatttcc aagatttcga tacctgtccg
    15661 ccgcccgaag acttgggtat gctgcttgaa gaagcgggtt tcgatacgat ggaaatcggc
    15721 aacaccctga tgatgatgga agtattgctc aacagctccg aattttccgc cgaacccgcc
    15781 gacagcggcg cattgcgcgt gtacagcaaa gaagaaaccg acaacctgcc gcaggaagtg
    15841 atggggctga tgcagtatct gattgaagaa aaagccgtca gctgcgaaca gcgggaaatc
    15901 atcatccacg cgctcatgca cattccgggc gacgaaatta ccgtagatac cgccaaagtg
    15961 ctgaccctgc tgcttttatg ggcaaacaag agcgagctgc ccgtgttggt cggcgacgag
    16021 ctgatgagcg cgcttttact cgacaacaaa cccacgatga actgaagcgg cttcagacgg
    16081 cccgcccgag gccgtctgaa acgccggcat caaaaccacc atccagagaa cgacaaatgg
    16141 cgaaaaacct attaatcgtc gaatccccgt ccaaagccaa aaccctgaaa aaatatttgg
    16201 gcggcgattt tgaaatcctt gcatcctacg gacacgtccg cgacctcgtc cccaaaagcg
    16261 gcgcggtcga tcccgacaac ggctttgcga tgaaatacca actcatcagc cgcaacggca
    16321 aacacgtcga tgccatcgtc gccggtgcca aagaagctga aaacatctac ctcgccaccg
    16381 acccggatag ggaaggcgaa gccatttcct ggcatctttt ggaaatcctc aaatccaaac
    16441 gcggcttgaa aaacatcaag ccgcagcgtg tcgtgttcca cgaaatcacc aaaaacgccg
    16501 tgctcgatgc cgttgcccat ccgcgcgaaa tcgaaatgga cttggtcgat gcgcaacaag
    16561 cccgccgtgc tttggactat ttggtcggct ttaacctttc gccattgtta tggaaaaaaa
    16621 tccgccgagg tttgagcgcg gggcgtgtgc aaagccctgc tttgcgcctg atttgcgaac
    16681 gcgaaaacga aatccgcgcg tttgaagcgc aggaatattg gacggtgcat ctcgacagcc
    16741 acaaaggccg cagcaagttc accgccaaac tcgcccaata caacggcgcg aaactcgaac
    16801 aattcgacct gccgaacgaa gccgcgcaag ccgacgtatt gaaagaactc gaaggcaaag
    16861 aagccgtcgt taccgccatt gaaaagaaaa agcgcagccg caatcctgcc gcgccgttca
    16921 ccacttctac catgcagcag gatgccgtgc gcaaactcgg cttcaccacc gaccgcacca
    16981 tgcgtaccgc ccaacagctt tatgaaggta tagacgtagg gcagggcgcg atcggtctga
    17041 ttacctatat gcgtaccgac agcgtgaatt tggcggatga agcgttaacc gaaatccgcc
    17101 attacattga aaacaaaatc ggcaaagaat atctgccgag tgccgccaaa caatacaaaa
    17161 ccaaatccaa aaacgcccaa gaagcccacg aagccatccg tccgacttcc gtgtaccgca
    17221 cgcccgaaag cgtcaaaccc ttcctgagcg cagaccagtt caaactctat caaatgattt
    17281 ggcagcgcac cgtcgcctgt cagatgacgc ctgccaaatt cgaccaaacc accgtcgata
    17341 ttaccgtcgg caaaggcgta ttccgcgtaa ccggacaagt gcaaaccttc gcaggctttt
    17401 tgagcgtgta tgaagaaagc agcgatgacg aagaaggcga agacagcaaa aaactgcccg
    17461 aaatgagcga aggcgataaa ttgcccgtgg acaaactcta cggcgaacaa cactttacca
    17521 ctccgccgcc acgctacaac gaagccacgc tggttaaagc cctcgaagaa tacggcatcg
    17581 gccgcccctc gacctacgcc agcatcatct ccacgctcaa agaccgcgaa tacgttaccc
    17641 ttgagcaaaa acgcttcatg cccaccgaca caggcgacat cgtcaacaaa ttcctgaccg
    17701 aacacttcgc ccaatacgtc gattaccact ttaccgccaa actcgaagac cagcttgacg
    17761 aaatcgccgg cggcaaacgc caatggattc ccttgatgga caaattctgg aaaccgttca
    17821 tcaaacaagt ggaagaaaaa gaaggcatcg aacgcgccaa atttaccacg caggaacttg
    17881 atgaaacctg cccgaaatgc ggcgaacaca aactgcaaat caagttcggc aaaatgggcc
    17941 gtttcgttgc gtgtgccggc tatcccgagt gcagctacac gcgcaatgtc aatgaaaccg
    18001 ccgaagaagc cgcagagcgc atcgccaaag ccgaagccga acaggcagaa ctcgacggac
    18061 gcgaatgtcc taaatgtggt ggtcgcctag tgtacaaata cagccgaacc ggcagcaaat
    18121 tcatcggctg cgccaactat ccgaaatgca aacacgtcga gccgctggaa aaaccgaaag
    18181 ataccggcgt ccagtgtccg caatgcaaaa aaggcaacct cgtcgagcgc aaatcccgct
    18241 acggcaaact gttttacagc tgcagcacct atcccgactg caactacgcc acctggaacc
    18301 cgcccgtcgc cgaagaatgc ccgaactgcc attggccggt cttgaccatc aaaaccacca
    18361 aacgctgggg cgtcgaaaaa gtctgcccgc aaaaagaatg cggctggaaa gaacagattg
    18421 aaccgcccgc gccgaaggag taagattagg ttggtttgaa agagaaaagg tcgtctgaaa
    18481 aattttcaga cgacctttgc ttttctgtga ttggtttatt tgaatccgcg tgttgtttta
    18541 aagtccgata aaatccggtt catttcaggc acaaacaagg cgatgtaatc gtaagataga
    18601 ccgcgactgg cactgggatg gggaaagcag acgacttcgc aatcttcaaa cgattggaat
    18661 ttgacattga aacgtgtacc gtcaaattct ttttgcaccg tctccagcgg tttggtctgc
    18721 ttaccgacca actgctcgaa gcgtggcagt acattttggt tgttcagaaa atccgccaac
    18781 ctgctgccca tgaagaggat gactttcgga cgcagttttt cgatgtggta gagaaaatta
    18841 tcgatgtgct cgggttgtgt gaacttgtcg ggattgtcga tagtgttgcc ctgtgtagca
    18901 gcccagttgg tttgaaccag ggatttttca aatgcaccgc ccaatccatt ttcgtctaag
    18961 gggtgtcccc acatttcaaa ccaatttttt atcgtattgt cgtaacgcca cttttttgcc
    19021 tgctctccga aatagaggga tttgtttgca aatgtatggt cgattttgtt ttcagggagt
    19081 ttgtattcac ctgctacata agcagcctca tcggctttac tccaacccca ttcatagcca
    19141 caaatcatta agccatgttt gtcgttgtag cctttgaaca ggctgttgct caaattcaaa
    19201 tccttcatca tgaactcttc cttttaaaat ttaagagcga ttgacttcaa tgtttttaga
    19261 tggggtggaa aaatccttgt gtaggcaaca taaattcaat aaatttcttg ataattcgaa
    19321 acctactaat agcgcaccta taaaagcttt ttcattacgt tcagcatgac ggtcacgtcg
    19381 ttcatatttt ttacgcttgc tgttccctgt tattacagct aagccaagtg atatggcgag
    19441 aattgcccaa acaatagtac ttaataacaa ttttccccat actatcaata gggaaagaaa
    19501 aaacctttta gtattaggtc gatagattat aatccatgcc catgaaaatg caataagagt
    19561 tatacataag atacaagctg ataggatttc tttattttta attaaataac ttagggcgat
    19621 gaggatgaca ggtgtcaaga aaacaatagt tacatcccga tagctataaa agaaaactgc
    19681 cccattttga atgtggaaat gtgcacagaa tccaatatag ctaaggataa tagttaatat
    19741 aataaaaaaa gaccaccaag ggtgaagaga taggaattcc atgttttccc tttttttgta
    19801 aaaaggaaaa aatcttatct aatagttaat tgcctaacca gccagaagaa gtttaaaatc
    19861 tatcccaata attcaaccat ctatacagaa agttcagctt atggaaaccc acgaaaaaat
    19921 ccgcctgatg cgcgaattga ataaatggtc ctaggaggat atggcggaaa agctggcgat
    19981 gtcggcaggc gggtatgcca aaatcgaacg aggcgaaacg cagttgaata tcccgcgttt
    20041 ggagcagttg gcgcagattt tcaaaattga tatgtgggac ttgctcaaat cgggcggcgg
    20101 cgggatggtg ttgcagatta atgaaggtga tagtggtggc gatattgcgt tgtatgcgtc
    20161 gggtgatgtt tcgatgaaaa tagaattttt aaaaatggag ttgaaacact gcaaagaaat
    20221 gttggaacaa aaagacaaag aaatcgagct gctccgcaag ctgaccgaaa ccgtttaaac
    20281 agatatgccg tctgaaaaaa gttttcagac ggcatattct ttgacaggtc ttgtataata
    20341 ccgtttgaac ttacaggttt ttgattatgg cggcaggcaa acataccaaa cacagcaacc
    20401 gggtacgcat tatcggcggg caatgccggg gcaggaaatt gagtttcaca tccgccgacg
    20461 gactgcgtcc gacacccgac agcgtgcgtg aaaagctgtt taactggctg ggacaggatt
    20521 tgacgggtaa aacggttttg gatctcttcg gaggcagcgg cgcactcggt atagaagccg
    20581 cttcgcgcaa cgccaaacgc gtgctgattt cggataacaa ccgccaaacc gtgcagacct
    20641 tgcagaaaaa cagtcgcgaa ctgggtttgg ggcaggtgca aatcgtcttt tcagacggca
    20701 tcgcatattt gaagaccgta tccgaacagt ttgatgttgt ctttctcgac ccgccgtttg
    20761 catggcagga ctggcaaatc ctgtttgatg ccttgaagcc gtgcctgaac ccccgggcat
    20821 tcgtctatct cgaggcgggt acgctgccga atattcccga ttggctgacg gaatatagag
    20881 aagggaaatc ggggcagagt acatttgaat taagggtttt ccaagtggct gaataatatg
    20941 cgctttgata atcatttccg agttgtaaac attcgtttgc aaccgtccgg ttcaaaaaaa
    21001 ccttgtgcta taatccgcgc ccgcccggtt ttgataattt agtggaaaag gaaaagaaat
    21061 gtcgcttttt attaccgacg agtgcatcaa ctgcgacgta tgcgaacccg aatgccccaa
    21121 tgatgccatt tcccaaggcg aggaaattta cgaaatcaac cccaacctct gcacgcagtg
    21181 cgtcggacac tacgatgagc cgcagtgcca gcaggtttgc ccggtggact gcatcctgat
    21241 tgaccaagaa catcccgaaa cccatgacga gttgatggcg aaatacgaaa agattatcca
    21301 gtttaaataa attcttttta aaacatcaaa ttatgtctgt tttgaaataa aatcaaaaaa
    21361 aaacttgacg gaaaagcaag ccgctaataa actaacgttc tcttttggag ggattcccga
    21421 gcggtcaaag ggggcagact gtaaatctgt tgcgaaagct tcgaaggttc gaatccttct
    21481 ccctccacca aaattcttac ttggggcagt agcgagtaat gcgggtgtag ctcaatggta
    21541 gagcagaagc cttccaagct tacggtgagg gttcgattcc cttcacccgc tccaaacaat
    21601 taggcccatg tagctcaggg gtagagcact cccttggtaa gggagaggtc ggcagttcaa
    21661 atctgcccat gggcaccatc cctcgattat tcatttcttt aaggcttaga tatataggat
    21721 attg
//
LOCUS       DAWFIE010000027        21597 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWFIE010000027 DAWFIE010000000
VERSION     DAWFIE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21597
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.027"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..380)
                     /locus_tag="WGQ71_000954"
     CDS             complement(<1..380)
                     /locus_tag="WGQ71_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706987.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="WGQ71_000955"
     CDS             complement(438..803)
                     /locus_tag="WGQ71_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706988.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1330)
                     /locus_tag="WGQ71_000956"
     CDS             complement(785..1330)
                     /locus_tag="WGQ71_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706989.1"
                     /translation="MKKQITAAVMMLSMIAPAMANSMDNQIFENQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            complement(1493..1765)
                     /locus_tag="WGQ71_000957"
     CDS             complement(1493..1765)
                     /locus_tag="WGQ71_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706990.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYVIGIINVIIS
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
     gene            complement(1834..2268)
                     /locus_tag="WGQ71_000958"
     CDS             complement(1834..2268)
                     /locus_tag="WGQ71_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706991.1"
                     /translation="MKKQITSAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGEWIPNAVGGIMGGVGGHFSYMASAIASGSYNREAHWTAIGGGALIG
                     AMNPINGGRALINGMRGVAVGTVAGGINGYAGSTGKNTDIRR"
     gene            complement(2311..3180)
                     /locus_tag="WGQ71_000959"
     CDS             complement(2311..3180)
                     /locus_tag="WGQ71_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ0706992.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLKTSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            3344..4267
                     /locus_tag="WGQ71_000960"
     CDS             3344..4267
                     /locus_tag="WGQ71_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0706993.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKTMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSRFCL"
     gene            complement(4345..4986)
                     /locus_tag="WGQ71_000961"
     CDS             complement(4345..4986)
                     /locus_tag="WGQ71_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0706994.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPDVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(5041..5832)
                     /locus_tag="WGQ71_000962"
     CDS             complement(5041..5832)
                     /locus_tag="WGQ71_000962"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ0706995.1"
                     /translation="MARHPYRRLRPAKSSFPEVGISEEGNIRSLHLGSDTIQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            5998..6070
                     /locus_tag="WGQ71_000963"
     tRNA            5998..6070
                     /locus_tag="WGQ71_000963"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6031..6033,aa:Thr,seq:tgt)
     gene            6108..6183
                     /locus_tag="WGQ71_000964"
     tRNA            6108..6183
                     /locus_tag="WGQ71_000964"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6141..6143,aa:Thr,seq:tgt)
     gene            6325..7116
                     /gene="panB"
                     /locus_tag="WGQ71_000965"
     CDS             6325..7116
                     /gene="panB"
                     /locus_tag="WGQ71_000965"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ0706996.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARSAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMHGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            7106..7282
                     /locus_tag="WGQ71_000966"
     CDS             7106..7282
                     /locus_tag="WGQ71_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706997.1"
                     /translation="MQTEQGLLLMPSESRFRRHFVLPCAARIIGAFCRAGSPKDKDYR
                     YANHTYHSRTARVA"
     gene            7239..8075
                     /locus_tag="WGQ71_000967"
     CDS             7239..8075
                     /locus_tag="WGQ71_000967"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ0706998.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRVDTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            8240..10078
                     /locus_tag="WGQ71_000968"
     CDS             8240..10078
                     /locus_tag="WGQ71_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0706999.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVVKVFRKQQR
                     YSEEEIKNERVRLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAAELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            10218..10799
                     /locus_tag="WGQ71_000969"
     CDS             10218..10799
                     /locus_tag="WGQ71_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ0707000.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETQEQCAVRTR"
     gene            10809..11654
                     /gene="ispE"
                     /locus_tag="WGQ71_000970"
     CDS             10809..11654
                     /gene="ispE"
                     /locus_tag="WGQ71_000970"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ0707001.1"
                     /translation="MNIADGRQAFSAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            11662..11737
                     /locus_tag="WGQ71_000971"
     tRNA            11662..11737
                     /locus_tag="WGQ71_000971"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11695..11697,aa:Gln,seq:ttg)
     gene            11750..11825
                     /locus_tag="WGQ71_000972"
     tRNA            11750..11825
                     /locus_tag="WGQ71_000972"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11783..11785,aa:Gln,seq:ttg)
     gene            11834..11909
                     /locus_tag="WGQ71_000973"
     tRNA            11834..11909
                     /locus_tag="WGQ71_000973"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11867..11869,aa:Gln,seq:ttg)
     gene            12076..13059
                     /locus_tag="WGQ71_000974"
     CDS             12076..13059
                     /locus_tag="WGQ71_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ0707002.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            13126..13698
                     /locus_tag="WGQ71_000975"
     CDS             13126..13698
                     /locus_tag="WGQ71_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ0707003.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(13823..14992)
                     /locus_tag="WGQ71_000976"
     CDS             complement(13823..14992)
                     /locus_tag="WGQ71_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ0707004.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNRPTAHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWACLTGQ"
     gene            15140..16666
                     /gene="ilvA"
                     /locus_tag="WGQ71_000977"
     CDS             15140..16666
                     /gene="ilvA"
                     /locus_tag="WGQ71_000977"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ0707005.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGDETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(16723..17796)
                     /locus_tag="WGQ71_000978"
     CDS             complement(16723..17796)
                     /locus_tag="WGQ71_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ0707006.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKIHAVGALTHILVKHDKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(17834..17941)
                     /locus_tag="WGQ71_000979"
                     /pseudo
     CDS             complement(17834..17941)
                     /locus_tag="WGQ71_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(17937..18758)
                     /gene="cysW"
                     /locus_tag="WGQ71_000980"
     CDS             complement(17937..18758)
                     /gene="cysW"
                     /locus_tag="WGQ71_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ0707007.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKL"
     gene            complement(19136..19972)
                     /gene="cysT"
                     /locus_tag="WGQ71_000981"
     CDS             complement(19136..19972)
                     /gene="cysT"
                     /locus_tag="WGQ71_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ0707008.1"
                     /translation="MLALKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGASRWTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            20111..20218
                     /locus_tag="WGQ71_000982"
                     /pseudo
     CDS             20111..20218
                     /locus_tag="WGQ71_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            20264..20596
                     /locus_tag="WGQ71_000983"
     CDS             20264..20596
                     /locus_tag="WGQ71_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ0707009.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            20929..21438
                     /locus_tag="WGQ71_000984"
     CDS             20929..21438
                     /locus_tag="WGQ71_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ0707010.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT         5224 a         5015 c         5842 g         5516 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcgccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccctctcag tctccttcat ctccttttga gaaagttccg
     1201 ccaactgcat cggcgcatct gcccgcgtat ggaatacttg gttttcaaat atctgattgt
     1261 ccatgctgtt tgccattgcg ggggcaatca tagacagcat cattacggct gcggtaattt
     1321 gttttttcat aataactcct ttggattaca aggttggaaa atcaaagccc tgcttagaac
     1381 gtatgttgca cacccaattt caataggtaa atcagattgc aaatccagca atttgaatat
     1441 tgtcattgtt ccgtgcaaaa gggattttta ttgatgagtt gtgtactggg tttcagcttg
     1501 gctttttaga taatcttttc acaagaacat acatcaatac tgtccaaaaa aaaagatttg
     1561 ataaatatat tgcaacatat tttattccac ctccatccga aacagaaacc aatatatttt
     1621 taattaatac atagctgatt attacattta ttattcctat aacatataag gatcttgcca
     1681 aaattttttt tgattttatt ttaggaatat ctcttatcca tgctaaaata cagcccaatg
     1741 tcgaaaagaa aataacagca aacactacct tacctcctct cacttccaac caaaccgata
     1801 gcaggtttgg ttggaagttg gtttatttat tatttaacgg cgaatgtcag tgttcttacc
     1861 cgtagaacct gcataaccat ttatgccacc agcaacagtc cccactgcta cacctcgcat
     1921 accattaatc agtgctcttc ctccgttaat agggttcatc gcaccaatta acgcaccacc
     1981 tccaattgct gtccaatgtg cctctcgatt atacgagcca ctagcaatag cactagccat
     2041 atagctgaaa tgccctccaa ctcctcccat aatgcctcca acagcattag gaatccactc
     2101 cccctcagtc tccttcatct ccttttgaga aagctccgcc aactgcatcg gcgcatctgc
     2161 ctgcgtgtgg aacacttggt tttcaaatgt ctgattgtcc aagccgtttg ccattgcggg
     2221 ggcaatcata gacagcatca ttacggctga ggtgatttgt tttttcataa taactccttt
     2281 ggattacaag gttggaaaat caaaacctgc ttaaaatgta tgctgtacgc caaatttcag
     2341 ttcggaactg ctttgccctg aaacgttgaa acgtgcggat gcgtttaaag ccgtggtttt
     2401 ggtgaaaccg aaacctgtgc cgaaatgagc gtaggtggat gtgtttcttg cggtttcttt
     2461 tttgccgtct atgcggtcgg gctgtgtgcc cagccattgg atgcctccgg tcaggctgat
     2521 tctgtcgttg gcggcaaatg agatgttggg gttgagcagc aggtagttgc ccgatttgta
     2581 gcggatgccg tctgaaaggg ttttgctgcc gttgatgcgg taggcggcgg tgagggaaag
     2641 gacaatcgga tctatggctt tgtaggtggt ggcgccgatg agccaggatt ttcccgacga
     2701 ggctttgttg cgcgattttt cgtaaaccgt gctttcaaga aagctgatta gggcggggtt
     2761 tttgtcgtct ttaaggaaag tgtggctgat gccgagggat acgtcggaca tccgtttgtt
     2821 gcgggttttg ccgttgccgt cgagtttgcg ttcttcgtgc cacagatagc tgccgctgcc
     2881 gtaaatgtcg gtattcccgg tcagtccgta gcgcaaaccg agcgtgccaa cgagcatatc
     2941 ggtattgctg ccgttttctt ggatttcggt cggaatgggg ataaacgagg ttgcgccggt
     3001 ttggatgtaa accggtgcgg caagttcggc gcggttgttt tcgctgttca ggtaggtaag
     3061 ggaagttttc agtttccatt tgcccttgtc ggtcattatg tcttcaatcg tcaagggcag
     3121 gtcggcataa gcggataaag gcaggatggc gggcaaggcg ggcaaaaaga tgcgcttcat
     3181 atttctccta ttgatattaa ttcttattta ctgataataa tgaatatccg ataatcttgt
     3241 ctaaataaat ttttaaatca taaaaaacaa tatgtttgtt tttatcaatt atgttttgat
     3301 atgccgtctg aaaagtttgt gaaaaacggt tacaatccgc cgcatgaaaa aacgcaataa
     3361 tcctcttccg ctgttgaacg gtgtcaaacc cagttatttg gtgctgccgc atgaaaagca
     3421 gttttacggg ctgccgctgc tgcattttct gtgcatccgc tttccttttg tgggcgcgga
     3481 cgattggcgc aggcggttga acagcggttt tgtggtcggt tcggatggtg cggcgttgga
     3541 cgaacattct ttgttcgagc cgggtaagac gatgttttat tatcgggaaa ccagcagtga
     3601 ggatgagccg cgtattccgt ttgaagaaaa gattttgcat attgatgagc atttgattgt
     3661 ggtggacaag ccgcattttc tgcccgtcat ccccagcggg cgttttttga gggaaacgct
     3721 gctcacacgt ctgcgcctgc gtcctgaatt gcagcatttg aatgttgagg acattacgcc
     3781 gctgcaccgc ttggacaagg atacggcagg cgtgatgctg ctgtcgcata atcctgccac
     3841 gcgcggggct tatcagacga tgtttcaaaa caaaacggta tggaaaacgt atgaggcact
     3901 tgcgccgaca aggacggatt tgccgtatcc gctcgatgtg gtttcgcgtt tggtgagggg
     3961 tgagaaattt tttacgacgc aagaggcgga aggcgaacca aacgcacata cgacggtcga
     4021 actgattgaa aacagggggg aattcagcct ttaccgcctt acgccgcata ccggcaagaa
     4081 acaccagttg cgcgtgcata tgatgggttt gggtatgccg ctgttgaatg acgcgctcta
     4141 tcccgtgccg tctgaagcgg gcagcgagga ttatcggaaa cctttgaagt tattggcaaa
     4201 aaagattgcg tttgcagatc ctttgtcggg tcgggaaagg gtgttttgca gtaggttttg
     4261 cctataatga gagggaatgc aaacccgtat cggcagatgc cgccgatacg ggtttgatat
     4321 tatcgatgcc gtctgaaagg ggttttatcc gttcaggcat acgggcgcga agcggaggat
     4381 ggtgtcgcgc aggacgatga gtttgccgtg aaagaaatgc gtggaaccgg cgatggtaat
     4441 gacgggcaaa tcttgcggtt ctgcccattt caatgctttc ccgatttcga cgacttcatc
     4501 ttccgcgccg tgtatcatca gcgtttttgc aacgtcggga acggctgacg gttccggacg
     4561 gccggtatag tggcagactg ccgcgccgat gagcagcaat aaatcgggta tgcgcgcttg
     4621 tgcggcaaat gtggcgacat aaccgccgaa ggagaagccg gataaggcaa attcgggggc
     4681 ttcgggatgt tgggcacggg catagtcgat gacggcgagg cagtcttgcg tttcgccgcg
     4741 tccgtaatcg tgtgtgcctt cgctgccgcc tacgccgcga aggttgggca ggtagcagtg
     4801 gaagccgagt ttgcttaagg ctttggcggc agtttggata actttgttgg tgtttgttcc
     4861 gccctggagg gggttgggat gattgatgac ggcaacaccg cgtgccggta cttgttcgga
     4921 cgggatatgg atggtttcta aaatgccggc aggaccggat atgtgtatgg tttcgggttt
     4981 cagcataatg atttcctctt gattttccgc atgttccaat gcagccgtga agatagttgc
     5041 ttaaaggtgg aaatgcttgc cgttgttggg gttggacgct ttcaggccgg caagcatacg
     5101 gtggaagtcc aatccgtatg cgttgctcag tttgtcggca cgttttttga gtttgtctat
     5161 gttttgctct ttggggctgc tttggaaggc cattaccgcg acattgccgt ggctttcggc
     5221 aggaagttcc aagacgcgcc cttcaaaaac gctcaacaac cgttcgatga agcgttggta
     5281 gcgtttgtcg ccgctccacc agttggttac gaatatgccg tctgaagaga gtgcgtttcg
     5341 gcagtctcgg aaaaacggtt cttcaaccag cgcatcgatg atttgttcgc cgtcgaaccc
     5401 gtccaccaaa atcacatcgg tgttgtggcg gaagactttg atatattccg caccgtctgc
     5461 ttcaataatt tcaaatttct cgccctcgaa aggcaactca aacaggttgc gggcaatggc
     5521 gataacctgc gggttgatgt ccacggcggt ttggcgcgtg tcgggcaggt aggtgtctat
     5581 ccagcgtgca aacgaaccgc cgcccaagcc gatttgggtg atatgctgcg gaagagcgtc
     5641 ggtaaacagc agccatccca tcatggcacg gctgtaagag agtaccagct cggacgggtg
     5701 gtcgaggttc attgagcttt ggatggtgtc gctgcccaag tgaagcgaac ggatattgcc
     5761 ttcttcggaa atgccgactt cgggaaagct ggattttgca ggacgcaggc ggcggtaggg
     5821 atgtcttgcc atcggtgtga acggtcggtt tgaaaggcag atattttatc agaatgccga
     5881 tggttgtcct gcttcaaatt aatttctttt caaataaatt acttattcgg atttgccggg
     5941 gctttcggat aaattccttg ccaaggtgcg gcattgcctg cataattcgc tttctttgcc
     6001 ggtatagctc agttggtaga gcacctgact tgtaatcagg gggtcccgag ttcgactctt
     6061 ggtgccggca tcatttctgc tgatggtgtt gcaaaacatt tcagtaagcc ggtatagctc
     6121 agttggtaga gcacctgact tgtaatcagg gggtcccgag ttcgactctt ggtgccggca
     6181 ccagatttga cagtccgatt gtgtaaaaca gtcgggctgt tttgcatttg cggcacggtc
     6241 ggtggcaggg ctgcgggcat ttcactataa tagcccgttt caaactgaaa aggccgtctg
     6301 aaaagggcgg ggtaacaata tctgatgatt actgtgaaca cactgcaaaa aatgaaggcg
     6361 gcgggcgaaa aaatcgctat gctgaccgct tacgaatcca gttttgccgc actgatggac
     6421 gatgccggcg tggaaatgct gctggtcggc gattctttgg ggatggcggt tcaggggcgg
     6481 aaatcgacgc tgcccgtcag cctgcgcgat atgtgctatc acaccgaatg tgtagcacgc
     6541 agtgcaaaaa atgcgatgat tgtcagcgat ttgccgtttg gtgcatatca gcagagtaag
     6601 gagcaggcgt ttgccgccgc cgccgaactg atggcggccg gcgcgcatat ggtcaaactc
     6661 gaaggcggcg tgtggatggc ggaaacgact gaatttctgc aaatgcgcgg tattccggtc
     6721 tgtgcccaca tcggtctgac gccgcaatcc gtgtttgcct ttggcggata taaggttcag
     6781 gggcgcggcg gcaaggcgca ggcgttgctt aacgatgcca aggcgcatga cgatgcgggg
     6841 gcggcggtcg tgctgatgga gtgcgtaccg gcggaactgg caaaaaaggt aactgaaact
     6901 gtttcctgtc cgaccatcgg catcggtgcg ggcgcggatt gcgacggtca ggttttggtg
     6961 atgcacgata tgctgggcat tttcccgggt aagacggcga aattcgtcaa aaactttatg
     7021 cacgggcaaa gcagcattca ggcggcggtt cgggcgtatg ttgccgaagt caaagccaaa
     7081 accttccctg ctgcggaaca tatttttgca gactgaacaa ggtttgctgc tgatgccgtc
     7141 tgaaagccgt ttcagacggc attttgtttt gccgtgcgcg gcgcgtataa tcggcgcgtt
     7201 ttgtcgggca ggaagcccga aggataagga ttaccgttat gcaaatcata cataccattc
     7261 gagaactgcg cgcgtggcgt aaaaatgcgg gaaaggtggc atttgtgccg accatgggca
     7321 atctgcatga agggcatctt gcgcttgtgc gtgaggcgaa aaaacgcgcg gacagtgtcg
     7381 tggtcagcat tttcgtcaac cgcctgcaat tcggtcaggg cgaggatttc gacaaatatc
     7441 cgcgtacttt gcaacaggat gcggacaagc tggaagccga gggtgttgcc gttgttttcg
     7501 cgcccgatga gaaagaactc tatccgaacg tggaacagcg ttacaacgtc gaaccgccca
     7561 atctgcaaaa tgagttgtgc ggcaagtttc gtccgggaca tttccgcggg gtggcaacgg
     7621 tagtgtccaa gctgttcaat attgtcgcgc cggatgttgc ctgtttcggc aaaaaagact
     7681 atcagcagct tgccgtgatt aaaggtttga ccgaggattt gaattttgat gttgaaatag
     7741 tgcctgtcga tacggggcgc gcggaagacg gtttggcact ctccagccgc aaccagtatt
     7801 tgagtgcggc ggaacgcgac gaagcaccgc gcctgtaccg cgaattaaag gctgttgccg
     7861 aatccttggc gcagggcagt ttggattatg caggtttgga aaaacgtgcc gtccaatccc
     7921 tgacagaata cggctgggtg gtcgattatg tcgaaatccg ccgcgtcgat acgctcgaag
     7981 tggcgcgggc gggagataag aaactggtgg tcttggccgc cgcccgtctg gggacgacgc
     8041 gcctgattga caatttggaa ataaaactcc cttaaaccgc aagcgtcggg aatgccgtct
     8101 gaagcggatt tgcgtttcag acggcattta tttttttgaa cggggtttcg caaatctaca
     8161 aacgattctg cttgtgataa agttacgcct gattatatgc cgtctgaagg ttcggacggc
     8221 tgtcggataa aggaagatta tgttacccgc ccgtttcacc attttatctg tgctcgcggc
     8281 agccctgctt gccgggcagg cgtatgccgc cggcgcggcg gatgcgaagc cgccgaagga
     8341 agtcgtaaag gttttcagaa agcagcagcg ttacagcgag gaagaaatca aaaacgaacg
     8401 cgtacggctt gcggcagtgg gcgagcgggt taatcagata tttacgttgc tgggagggga
     8461 aaccgccttg caaaaggggc aggcgggaac ggctctggca acctatatgc tgatgttgga
     8521 acgcacaaaa tcccccgaag tcgccgaacg cgccttggaa atggccgtgt cgctgaacgc
     8581 gtttgaacag gcggaaatga tttatcagaa atggcggcag attgagccta taccgggtaa
     8641 ggcgcaaaaa cgggcggggt ggctgcggaa cgtgctgagg gaaagaggaa atcagcatct
     8701 ggacggactg gaagaagtgc tggctcaggc ggacgaagga cagaaccgca gggtgttttt
     8761 attgttggca caagccgccg tgcaacagga cgggttggcg caaaaagcat cgaaagcggt
     8821 tcgccgcgcg gcgttgaaat atgaacatct gcccgaagcg gcggttgccg atgtggtgtt
     8881 cagcgtacag ggacgcgaaa aggaaaaggc aatcggagct ttgcagcgtt tggcgaagct
     8941 cgatacggaa atattgcccc ccactttaat gacgttgcgt ctgactgcac gcaaatatcc
     9001 cgaaatactc gacggctttt tcgagcagac agacacccaa aacctttcgg ccgtctggca
     9061 ggaaatggaa attatgaatc tggtttccct gcacaggctg gatgatgcct atgcgcgttt
     9121 gaacgtgctg ttggaacgca atccgaatgc agacctgtat attcaggcag cgatattggc
     9181 ggcaaaccga aaagaaggtg cttccgttat cgacggctac gccgaaaagg catacggcag
     9241 ggggacgggg gagcagcggg gcagggcggc aatgacggcg gcgatgatat atgccgaccg
     9301 cagggattac gccaaagtca ggcagtggct gaaaaaggtg tccgcgccgg aatacctgtt
     9361 cgacaaaggt gtgctggcgg ctgcggcggc tgccgaattg gacggcggca gggcggcttt
     9421 gcggcagatc ggcagggtgc ggaaacttcc cgaacagcag gggcggtatt ttacggcaga
     9481 caatttgtcc aaaatacaga tgctcgccct gtcgaagctg cccgataaac gggaggcttt
     9541 gagggggttg gacaagatta tcgaaaaacc gcctgccggc agtaatacag agttacaggc
     9601 agaggcattg gtacagcggt cagttgttta cgaccggctt ggcaagcgga aaaaaatgat
     9661 ttcagatctt gaaagggcgt tcaggcttgc acccgataac gctcagatta tgaataatct
     9721 gggctacagc ctgctttccg attccaaacg tttggacgaa ggcttcgccc tgcttcagac
     9781 ggcataccaa atcaacccgg acgataccgc tgtcaacgac agcataggct gggcgtatta
     9841 cctgaaaggc gacgcggaaa gcgcgctgcc gtatctgcgg tattcgtttg aaaacgaccc
     9901 cgagcccgaa gttgccgccc atttgggcga agtgttgtgg gcattgggcg aacgcgatca
     9961 ggcggttgac gtatggacgc aggcggcaca ccttacggga gacaagaaaa tatggcggga
    10021 aacgctcaaa cgtcacggca tcgcattgcc ccaaccttcc cgcaaacccc ggaaataatg
    10081 caggtccatc ctttcagacg gcataaggtt tgccgggaag ctgcggcatt cgggcaaacc
    10141 gcacgcagtt cgcacgcgtt ttgcacggca cgccgaaccc atcggccggc aggatggcat
    10201 ccgttaagga aattctgatg aaacacactg tatccgcatc ggtcatcctg cttttgaccg
    10261 cttgcgcgca attacctcaa aataacgaaa acctgtggca gccgtccgaa cacacccgca
    10321 gtttcacggc ggaagggcgg ttggcagtga aagcggaagg gaaaggttcg tatgcaaatt
    10381 tcgattggac ataccaaccg cccgtggaaa ccatcaatat caatacccct ttgggcagta
    10441 cgctcgggca gttgtgtcaa gacagggacg gcgcattggc agtggacggc aaaggaaatg
    10501 tctatcaggc ggaaagtgcg gaagaattaa gcaggcagct ggtcggtttc aaactaccaa
    10561 tccaatatct gcatatctgg gcagatggca ggcgtgtggc gggcgcgcct taccgcatcc
    10621 tgccggacgg catattggaa caatacggtt ggactgtcgg cagaaccgcc gacagtgggg
    10681 ggcaagtccg aacgttgcaa ctgaataacg gaaatttgaa catcaggctg gttttcaccg
    10741 aaattggaat gccgtctgaa actgaaaccc aagaacaatg tgcggtacgc acgagataag
    10801 gcggacagat gaatattgcg gacggacggc aggcgttttc cgcacctgca aaactgaatc
    10861 tcgatttgag gattaccggc aggagggaag acggttatca caatatcgaa agcatattct
    10921 gcctgataga tttgcaggat accgtatatt tgaaaccgag ggacgacggc aaaatcatcc
    10981 tgcacaatcc tgtcggcgga ataccgcagg aagccgattt gagctaccgt gccgcatcgt
    11041 tgctgcaaaa atatgcgcgc aaccccgccg gcgtggaaat atggctggac aaaaaaatcc
    11101 cgacaggggc gggtttgggc ggcggaagct cggatgcggc aaccgttttg ctggtgttga
    11161 accgttggtg gcagtgcggt ctgacgcagc ggcagctcat tgattcgggc gcggctctgg
    11221 gggcggacgt accgtttttt attttcggca aaaatgcgtt tgcgcggggt ataggcgaca
    11281 ggctggacga aatggatatt ccgaaacagt ggtatgtcat cgtcaaaccg cccgtccacg
    11341 tttccactgc aaaaattttc acacacgaag gcttgacacg aaattccgcc tcaagcataa
    11401 tgccgacttt ccaaaacctg caaccgttta gaaatgacat gcaggcagtt gtatttaaag
    11461 aataccctga agtttggaaa gcctattccg agttgtcccg atatggattt gccttaatga
    11521 caggttccgg ggcatgtgta ttcacggcgt gtcaagatag gaatagcgca tacaatatat
    11581 accgacaagt ttcaggtttg tacgaggcat atttggcaga gggtctttca aagcatcctt
    11641 tgttgtccgt ataaacattg ttggggagtc gtcaagcggt taagacactg gattttgatt
    11701 ccagcatgcg aaggttcgaa tccttcctcc ccagccaagt caaacgagtt ggggagtcgt
    11761 caagcggtta agacactgga ttttgattcc agcatgcgaa ggttcgaatc cttcctcccc
    11821 agccagccct ttttggggag tcgtcaagcg gttaagacac tggattttga ttccagcatg
    11881 cgaaggttcg aatccttcct ccccagccag ataaaagcgt gtaagcctgc ttacacgctt
    11941 ttatttcata gaaataaaaa tattgaaatg cctttgtttg tgtcggatgt tgcaggtata
    12001 atgtcgggct tggtacaagc agagggaagc attgtgtttt ctgagcggaa gttaaacata
    12061 aaatcaggtg agaatatggc tgcgtacgac agtttgatgg tatttacagg caatgccaat
    12121 cccgaattgg cacaacgtgt tgtcaggcat ttggacattt ctttgggcaa tgcttccgta
    12181 tccaagtttt cagacggcga agttgccgtc gaactgttgg aaaacgtacg cgggcgcgat
    12241 gttttcatcc ttcagccgac ctgtgcgccg accaatgaca acctgatgga aatcctgacg
    12301 atggcggatg cactgaagcg tgcttcggca ggtcgtatta ccacagccat tccgtatttc
    12361 ggctatgcgc gccaagaccg ccgtccgcgt tccgtccgcg ttccgatttc tgccaaactg
    12421 gtggcaaata tgctgtattc ggcagggatc gaccgtgttt tgactgtcga tttgcatgcc
    12481 gaccagattc aaggtttctt cgatattccg gtggacaata tttatgccac cccgattctg
    12541 ttgaacgaca tcaaacaaca gcggattgaa aatctgaccg tcgtcagccc ggacatcggc
    12601 ggtgtcgtcc gcgcccgcgc cgtggcaaaa tccttgaatg ccgacttggc aatcatcgat
    12661 aaacgccgcc cgaaagccaa tgtggcggaa gtcatgaaca tcatcggcga tattcaaggc
    12721 agaacctgtc tgattgtgga cgatatgatt gacactgcaa atacgctgtg caaagccgcc
    12781 gtcgctctga aagagcgggg ggcggaacgt gtcttggcat atgccagcca cgccgtattc
    12841 tccggagagg cagtcagccg tattgcctca tccgaaatcg accaggtggt cgtaaccgat
    12901 accattcctt tgtctgaagc ggctaaaaac tgcgaccgta tccgtcaggt aacgattgcc
    12961 ggtctgttgg ccgaaaccgt ccgccgcatt agcaatgaag aatccgtctc atatcttttc
    13021 aatgaagaag tgatgacagg cagcatgttg ctgccataag cccgaagccg tcttaagctg
    13081 gtcgcggccg atgacggcga ttttacctaa cttggagtat ttaacatgac ttatgaaatt
    13141 caagcttctg ttcgtgaagc acaaggcact ggtgcgagcc gccgcctgcg tcgcgaaggc
    13201 caaatccccg gcattctgta cggtgaaggt caagagcctg ttgcaatcgc tgtggatcac
    13261 aaaaccgtat tctacgcatt ggaaaaagaa tctttccata ctgcgttgat taagttgtct
    13321 ctgaacggcg aaaccaaaga cgttatcgtc cgtgatttcc aaatgcaccc gttccgccgc
    13381 gaagttcaac acatcgactt ccaagctgtg aaagccgatc aacttgtacg catccgtgtt
    13441 cccctgcaca tcgttaacgc tgaaaattcc caagcggtca aactgcaagg cggccgcgta
    13501 tctctgttaa acacttctgt tgaagtagtt gctttgcctg ccaacatccc tgctttcttg
    13561 gatttggatt gtgccgaagt ggttgccggc gacattctgc acttgtcaga catcaaactg
    13621 cctgaaggtg tagaaagcgt ttccctgaaa cgtaacgaaa atctggctgt tgctaccgtt
    13681 accggtaaga aacgctaatt gatttcagca gcagggcggc gcgtatgcaa tacgtaccgc
    13741 cctgttgttt tatgccgtct gaaccgtgtt tcagacggca tttctttatt tgttggaaaa
    13801 acgggatatt tgaaacggca gattactgcc ctgtcagaca cgcccaaagc ctttgccacc
    13861 ggcttctttt ttttacattt tccagtgcga cgatttcttt ttcggcaatg gtgtatccgt
    13921 tttgtctgat tttgattttt cctaaaattt gccctttttt tactggggcg ggaatcggct
    13981 gtatggtttc tagaatttgt tctgccattt tcgcttcctt atgtggcaga gtgatgtagg
    14041 cttctttgag gaagcctgcg cggacggttt ttttgctgcc tccggaaatt tggatttggg
    14101 caacggtttt gcctttcgga tatattttgg gcgtatcgaa agcctgcaat gcccagttca
    14161 gtagcttgct gttgtccgat gcgcgtgttt ccgccgattc cgaacccaat gtgatgacaa
    14221 ggatgtgcct gccgttgccg gagtatgaca cggcaaggtt gtagccgccg ctttctgtgt
    14281 gtccggcttt cagaccgttt acattgttgt ccctatataa aaggatattg cggttgtttt
    14341 gctctatatt tttgaatttg aaagatttga tggaaaacag cgggtaatat tccggaaagt
    14401 cgcgcatcaa tgcttcagac agctgggcga ggtctttggc ggtggaaacc tgtccttctc
    14461 tactcaagcc tgtcggattt ttgaatacag tgttcttcat gcccaagcgt cgggcttctt
    14521 tgttcatttg ttgcacaaaa ttttcaatcg agccgttgcc cagccggccg gcaagggtta
    14581 gggcggcatc gtttgcggat agtgcaatca tgccttttaa gagtttgtcg gtgctgaccg
    14641 tatcgccggg acgtacaaac attctgcttc cttctgaagc ccatgcggat tcgggtattt
    14701 ttaagttttc ttcagatcgg atatttcccg atttcatgtt tttgaaaacc agatatgcgg
    14761 tcatcagttg ggttagtgcc gccggttcga caggggtatt gatgtttttg gcggataaag
    14821 tctgtctgct ttgaaggtcg ataacgatgt gtgccgctgt gagggtttcg ggtgtttgga
    14881 gcgtgggggc ggcgtgtgcc gtcggtctgt tgggcgcggg cgatgcagcc gttgcatgag
    14941 aaacgcctaa gatgatggaa agcaggacgg gcaggatttt atgtgctgtc atgaaatatt
    15001 ctaattgtgt gcgtgtttca gcctgccgat tatacgctta gggtgtctga tcgggcggat
    15061 ttttcttgat ttcgcgccgt cttgggcgta tggttttggg ttttgcgatt ttaataaacc
    15121 gattatccca tattgaatta tgaacacgcc ccttccttat tccgattacc tcatccgcat
    15181 cctgacggca tctgtctatg atgtcgcggt cgaaacgcct ttggaaccgg cacgcagcct
    15241 ttctgtacgt ttgaaaaaca acatcctttt gaaacgcgaa gatttgcagc cggttttttc
    15301 gttcaaaata cgcggcgcgt acaacaaaat gtccaagttg ccgaaagatg cgctcgcttg
    15361 cggcgtgatt gcggcaagcg cgggcaatca cgcacaaggc gtggcattgt ccgcacagcg
    15421 tttgggttgc cgtgccgtta tcgtcatgcc ggagactacg ccgaaaatca aagtggatgc
    15481 ggtcaaaagc cgtggcggcg aggtggtttt gcgcggtgtt tcatacaacg atgcttacga
    15541 ttatgcgatg gagctggcgg aacaggaagg gctgacctat atcgcaccgt ttgacgatcc
    15601 tgatgtgatt gcaggacaag ggacggtagg gatggaaatt gtcagccagc atcccgatcc
    15661 aatccgcgcc gtattcgtac cgataggggg gggcggtttg gcggcgggcg tggcggcatt
    15721 tatcaagcag gtccgtcccg aaatcaaagt tatcggcgtt cagaccaacg attcctgctg
    15781 tatgaagcag tcggtcgaag cgggtgaaat cgtccatttg aaagatgtcg ggctgttttc
    15841 agacggcact gcggtcaaag ttgtcggaga cgaaaccttc cgcctctgca aagaactttt
    15901 ggatgaaatc attacagtcg ataccgatgc ggtttgcggc gcggtcaagg atattttcga
    15961 tgacacgcgc agcattaccg agccggcggg cgcgttggcg ttggcgggtc tgaaagccta
    16021 tatcgcccga gaaggcgcgg aaaaccaaac cctgattgcc gttaccagcg gtgcgaatat
    16081 gaattttcac cgtttgcgcc atgtgtcgga gcgtagtgaa ttgggcgagg gcaacgaagg
    16141 tatttttgcc gttaccatcc ccgaagaacg cggcagcttc cttaagtttg tcaatatatt
    16201 gggaaatagg aatattaccg agttcaacta ccgctacgga gacgatgaaa aagcgcatat
    16261 ctttgtcgga cttcaagcgg caggcccgca ggatttggcg gttatcggca gccggttgga
    16321 tgaggcggga ttgcccaatg tcgacctgac tgatgatgag attgccaaaa tccatatccg
    16381 ttatatggtt ggagggcgga cggacaaagt agaaaacgag cgtttggtca gttttgagtt
    16441 tccggagcgt ccgggggcat tggcacgctt tttgaaccat atgcagggag ggtggaatat
    16501 tacgcttttc cattaccgca accacggtgc ggattacggg cggattttgg tcggtatcga
    16561 tgtgccgccg cacgatgccg ccgcatttga cggtttcttg gaaagtctgg gatacagcta
    16621 tcatgaggaa acgcaaaatg ccgcgtacaa gctgtttctt gcctgacgct tgaaagcaca
    16681 atgccgtctg aaagcctttc agacggcatt gcgctttcat ggttaaatcg aatattcaat
    16741 cagctcgttt tgagaaaaga cataaacctg ttttggaatc agcttcaatt ctttgccttc
    16801 ggcgattggg taacgcgcgg catcgctgcc agccagcgtg atatgtacgt cctgtttgtc
    16861 gtgttttacc agaatatgcg tcaatgcgcc gacggcgtgg attttttcga tttcggcaca
    16921 aatcatcggt gtttcgtgtt cggcggcgat ctgccattcg tgcgggcgga tatagccggt
    16981 ggcggtttgt tcctgccatt tgtattgcgc gtccaatttc cacgcgaagc cgttgtaatg
    17041 ccagaagcct ttttcgatgc gtccttcaaa agcgtcggtt tcgccgagga actcggtaac
    17101 gaaggcattt tcgggtttgc ggtaaatagc ttcggcgctg ccggtttgtt cgattttgcc
    17161 gtggttcatc acgacgattt cgtcggaaac ttcgagggct tcttcttggt cgtgcgtaac
    17221 cagaatgctg gttacaccca ggttgtgatg gatgtcgcgc agccaggtgc gtaattcttt
    17281 gcgtactttg gcatccaacg cgccgaaggg ttcgtccaaa agcaagagtt tgggttcgac
    17341 cgcaagcgcg cgggcgaggg cgatgcgctg gcgttgcccg ccggagagtt ggtgcggata
    17401 ggattttgcc aaatgagaga gctgcacgag cttgagtaat tcttcgactt tggcgcggat
    17461 ttgtcctttg gacgggcgtt cggacttggg caatacggtc aaaccgaaag cgacgttgtc
    17521 aaacacgttc atgtggcgga agagggcata gtgttggaat acgaagccga ctttgcgctc
    17581 gcgcacgtgc ttggcggtta cgtcttgccc atcaaacaga atcttgccgc cgtcggcgtt
    17641 ttccagtccg gcgataatgc gtaaaagcgt ggttttgccg cagccggacg ggccgagtag
    17701 ggaaacgagt ttgccggtgg ggacgttgag gttgatgttt ttcagcgcgt gaaaattgcc
    17761 gaagtgtttg tttaagtttt ggatggtgat actcatattg cgttcctttc ggcggcggcg
    17821 agttttttgt cttgtatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgcc
    17881 gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg atttaaattt aatccactat
    17941 aatttggtga tgatgttctg caccgccagc gtcgccagtg ctaaaagtgc caatacgccg
    18001 gagagggcga atgcgccggt aaagttgtat tcgttgtaga agatttcgac caaaagcggg
    18061 acggtgttgg tttcgccgcg tatgtgtccc gataccacgc tgaccgcgcc gaattcgccc
    18121 atcgcgcggg cgttggtgag gatgatgccg tagagtaacg cccatttgat gttgggcagg
    18181 gtaacgcgcc aaaacatctg ccagccgctt gcgccgagta tcaatgccgc ctgttcttcg
    18241 ctgtcgccct gagcctgcat cagcgggatg atttcgcgcg cgacaaaggg gaaggtaacg
    18301 aacagcgtcg ccagaataat gccggggatg gcgaagataa tctgtatgcc ttgcgcttcg
    18361 agccagccac ccaatgccgt gtgcgcgccg aacaataaga cgaacattaa accggccacc
    18421 acgggcgata cggaaaacgg caaatcgagc agggtagtca gcaactgctt gcctcggaag
    18481 tcaaaacggg tcagcagcca cgccatcgcc acacccaata cagcattgac gggaacgaca
    18541 atcagcgcgg taatcagcgt caatttgatg gcagaccacg cttcgggatc gtttaaggat
    18601 tttaggtaca aatcccaacc gccttttaag gcttcgtaaa acacggcgac gagcggcacg
    18661 accagcatca gcagcagaaa gcccagcgcg gcggcaatca gcaacacgcg cagccggcgc
    18721 ggttcggtca ggttgggatt ggcggaatag ggtttcatgg cggtggtgtt ctctggttgg
    18781 tttaaaatgt ttggaggtcg tctgaaaagg gattgagggg tttcagacga cctttttgtt
    18841 cattaagtag taggtcggat acttgtatcc gacaaggttg ctgaatcgca aatgtttgtc
    18901 ggatacaagt atccgaccta cgaattctgt gttttgggga gcggattcgt tttcagacga
    18961 ccttttatct tttatgctaa aagagaaata aaaaagtcta gctgccgtca ttcccgcgca
    19021 ggcgggaatc caccacaggg caacaggaaa acagaaaata aataaggcag ctgaaattca
    19081 ccaatggatt cccgcctgcg tgggaatgac ggtaacaggt atttcagacg ccgcctcaac
    19141 ccttcgcgcc cgaacgcctg cccaacgccc actgcatcac gttcagcgca aacagaatca
    19201 caaacgaaac cagcagcata aacaacgcca ccgccgacgc gccctgcacg tcgaactgtt
    19261 ccagcttgcc cgtaataatc agcggcagga tttcagaaac catcggaata ttgcccgcga
    19321 taaaaatcac cgagccgtat tcccccgttg cccgcgcaaa catcattccc gcgccggtca
    19381 agagtgccgg tgtgatttca ggcaagagga cacggcgaaa cgtagtccaa cggcttgcgc
    19441 ccaaagttgc cgccgcttcc tcatattcgc ccgacaattc ttccaatacc ggctgcacgg
    19501 cgcggacgat aaagggcagg ctgacgacga ccagcgcaat ccaaatgccg acgggtgtaa
    19561 acgcgatttt gatgcccaaa ggctcgaaaa aacggcctat ccaaccgttg ggcgcataca
    19621 gggttgccaa cgcgataccc gtaaccgccg tcggcagcgc aaacggcaaa tcgaccagcg
    19681 cgttcgctag acccttgccc ggaaattcat agcgcaccaa tacccacgcc accagcgtgc
    19741 cgaacacgac attggtcagc atcgcataaa acgacatccg caaactcagc cacaccgccg
    19801 ccaacacgtt cggctcggca atcgtgttcc aaaagccgcc ccagccgatt tccgccgcct
    19861 tcgccgccat catcgcaaac ggcaagacca caagcagcga caggcacaat acggtcagac
    19921 caaggctgag tttgaagccg ggcagtacgc cgggcgtttt gagcgctaac ataaaacaat
    19981 gctgaaaata aggaaaagga aggactactt taacgatgcc gtccgaaaaa cggaaagaat
    20041 ggaaagtttg gtgcaaagac gaatttgtta taaagcggtt ggcagtttct caagcgggcg
    20101 cgatgtttta aaatatagtg gattaaattt aaatcaggat aaggcgacga agccgcagac
    20161 agtacaaata gtacggcaag gcgaggcaac gccgtactgg tttaaattta atccactata
    20221 acgccttgtt ttgacggaaa accatcataa taaaggaaca cttatgcaga ttttatcttt
    20281 tcagccggac attgcggaac gtatgctgga aggtacggaa ggcgagtcgg tcaacgaaaa
    20341 cgcacaattc gtccgtacgg acaacggcta ttggattgcg tggcatgaag gcgtagcggc
    20401 actgcttgcg cccgatacgc cgccgggcat tccctgtttt tgggtggaag gcgcggaaag
    20461 ccttgaagag ttgtgcgtca tggtggaacg cggcgagttt gacgaagtgg aagagtttga
    20521 cggcgatgac gacgaatggc tcgaaacggc acagggttgc gggcaccacg gcgacgcttg
    20581 cgcctgcgga cattaaaggc attgcaggct tgccgcaagg gggggcaagg ctttgccgtt
    20641 ttttaaataa tcggtggatt gcatctgttt gcaatgggcg gaacgcttcc ctttgtattt
    20701 tattgaataa aaaaacttat cttggtatta taattaaggc agcatcaatt attttgggat
    20761 ggcaataaac gcaaagcatt gatttgcgcc gattgcagac ttattatagc aggttgcggc
    20821 gcggacttaa tgggaattgt ttatagagca tgatggtctg aaacatcatt aattaaaaat
    20881 accattaatc cgatttatat ttatttcaat ttcaatggaa aaacatcaat gacaatgatt
    20941 ttaagcattt taagcctgtt ttttatcatc agactgttat ttttagccgt ctctattaaa
    21001 catgaaaaag ccttgattgc caaaggggcg aaacaatacg gaaaaaccaa ttccacgctg
    21061 cttgcggcag ttcatacgct ttattatttg gcgtgttttg tttgggtatg gctttctgac
    21121 actgctttta atggcatatc cttgattggt acgctgacgg taatggcttc gtttgtgata
    21181 ttgtcattga ttattaagca gttgggggag atttggacgg tcaaaatcta tattttacca
    21241 aatcatcaaa ttaatcgttc gtggttgttt aaaacattcc gccaccccaa ttatttttta
    21301 aacatcatac ccgaactgat tggcatcgcc ttattatgtc aagcgtggta tgttttattg
    21361 attggtctgc ccatttattt gctggtctta tttaagcgta tccgacaaga agaacaggcg
    21421 atggcaacac ttttttaacc cgtttcatca attatagtgg attaacaaaa accagtacgg
    21481 cgttgcctcg ccttgccgta ctggtttttg ttaatccact atattccgcc atctctaaga
    21541 tttacagcga tacacgggtg atttaaggaa tgcccgaacc gtcattcccg ccacttt
//
LOCUS       DAWFIE010000028        21391 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWFIE010000028 DAWFIE010000000
VERSION     DAWFIE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21391
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.028"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..234
                     /locus_tag="WGQ71_000985"
     CDS             <1..234
                     /locus_tag="WGQ71_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0707011.1"
                     /translation="VIAQSEFVEFAKINKSENVIDCFSFFWNRRIKHDIWLISFSDNS
                     EMVIKESLKDGHKIYKFEFCEIVDNCNFDDVFV"
     gene            218..613
                     /locus_tag="WGQ71_000986"
     CDS             218..613
                     /locus_tag="WGQ71_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707012.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDPETDKFAKVSG"
     gene            complement(1113..1188)
                     /locus_tag="WGQ71_000987"
     tRNA            complement(1113..1188)
                     /locus_tag="WGQ71_000987"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1153..1155),aa:Asn,seq:gtt)
     gene            complement(1281..1640)
                     /locus_tag="WGQ71_000988"
     CDS             complement(1281..1640)
                     /locus_tag="WGQ71_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ0707013.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(1663..2307)
                     /locus_tag="WGQ71_000989"
     CDS             complement(1663..2307)
                     /locus_tag="WGQ71_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ0707014.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(2309..2845)
                     /locus_tag="WGQ71_000990"
     CDS             complement(2309..2845)
                     /locus_tag="WGQ71_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ0707015.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(2857..3954)
                     /locus_tag="WGQ71_000991"
     CDS             complement(2857..3954)
                     /locus_tag="WGQ71_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0707016.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3957..5393)
                     /locus_tag="WGQ71_000992"
     CDS             complement(3957..5393)
                     /locus_tag="WGQ71_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ0707017.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQYADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(5613..6395)
                     /gene="bioC"
                     /locus_tag="WGQ71_000993"
     CDS             complement(5613..6395)
                     /gene="bioC"
                     /locus_tag="WGQ71_000993"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ0707018.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(6383..7030)
                     /locus_tag="WGQ71_000994"
     CDS             complement(6383..7030)
                     /locus_tag="WGQ71_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ0707019.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPFA
                     VFKGTLENLTGNTRLKFERRMCGDKASFEDYQQFSARPFDEIHQELTALFAMIGQDRR
                     TDFIRWTNALVGSGDKIFMSANQHRYWTARCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(7040..8182)
                     /gene="bioF"
                     /locus_tag="WGQ71_000995"
     CDS             complement(7040..8182)
                     /gene="bioF"
                     /locus_tag="WGQ71_000995"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ0707020.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAACL"
     gene            complement(8204..8341)
                     /locus_tag="WGQ71_000996"
     CDS             complement(8204..8341)
                     /locus_tag="WGQ71_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HEZ0707021.1"
                     /translation="MATETNVAEISALDYEGRGVAKVGGKTVFIKRALLDCLMLGWFR
                     L"
     gene            8405..9481
                     /locus_tag="WGQ71_000997"
     CDS             8405..9481
                     /locus_tag="WGQ71_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ0707022.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(9688..11154)
                     /locus_tag="WGQ71_000998"
     CDS             complement(9688..11154)
                     /locus_tag="WGQ71_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ0707023.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(11352..12275)
                     /gene="speB"
                     /locus_tag="WGQ71_000999"
     CDS             complement(11352..12275)
                     /gene="speB"
                     /locus_tag="WGQ71_000999"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ0707024.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(12374..14266)
                     /gene="speA"
                     /locus_tag="WGQ71_001000"
     CDS             complement(12374..14266)
                     /gene="speA"
                     /locus_tag="WGQ71_001000"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ0707025.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYASTVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(14433..14717)
                     /locus_tag="WGQ71_001001"
     CDS             complement(14433..14717)
                     /locus_tag="WGQ71_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0707026.1"
                     /translation="MKKLLLAAVVSLSAATAFASDSAERQIYSDPNFEQNRVKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(14868..16676)
                     /gene="aspS"
                     /locus_tag="WGQ71_001002"
     CDS             complement(14868..16676)
                     /gene="aspS"
                     /locus_tag="WGQ71_001002"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ0707027.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPAMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYAEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIERTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(16734..17435)
                     /locus_tag="WGQ71_001003"
     CDS             complement(16734..17435)
                     /locus_tag="WGQ71_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ0707028.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKVALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(17578..18726)
                     /locus_tag="WGQ71_001004"
     CDS             complement(17578..18726)
                     /locus_tag="WGQ71_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ0707029.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPTVSAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(18887..19150)
                     /gene="rpsT"
                     /locus_tag="WGQ71_001005"
     CDS             complement(18887..19150)
                     /gene="rpsT"
                     /locus_tag="WGQ71_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ0707030.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            19453..20595
                     /locus_tag="WGQ71_001006"
     CDS             19453..20595
                     /locus_tag="WGQ71_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0707031.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5583 a         5437 c         4947 g         5424 t
ORIGIN      
        1 gttatagcgc aatctgaatt tgttgaattt gcgaagatta ataagtctga aaatgttatt
       61 gattgtttct cttttttctg gaatagaaga attaaacatg atatttggct aatctcattt
      121 tctgataatt cagaaatggt aattaaagaa tccctgaaag atggtcataa aatatacaaa
      181 tttgaatttt gcgaaattgt cgataattgc aattttgatg atgtattcgt ttgaagcgaa
      241 tgcaaatgca gtaaaaatat ctgaaactgt ttcagttgat accggacaag gtgcgaaaat
      301 tcataagttt gtacctaaaa atagtaaaac ttattcatct gatttaataa aaacggtaga
      361 tttaacacac atccctacgg gcgcaaaagc ccgaatcaac gccaaaataa ccgccagcat
      421 ttcccgcgcc ggcgtattgg cggggggcgg caaacttgcc cgcttaggcg cgaaattcag
      481 cacaagggcg gttccctatg tcggaacagc ccttttagcc catgacgtat acgaaacttt
      541 caaagaagac atacaggcac gaggctacca atacgacccc gaaaccgaca aatttgcaaa
      601 ggtctcaggc taagtgcgcc tgttgccgcc taaaaggcgg tccggatgcc tgattatcgg
      661 gtatccgggg aggattaagg ggatatttgg gtagaattag gaggtgttat ggagcgaaaa
      721 cagccgaaaa cctgtgttgg ggtttcggct gtcgggaggg aaaggaattt tgcaaaggtc
      781 tcaattagta tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca
      841 aatagtacgg aaccgattca ctcggtgctt cagcacctta gagaatcgtt ctctttgagc
      901 taaggcgagc caatgccgta ctggtttttg ttaatccact ataaaatcga gtttatccta
      961 gctgtccagg acagccccta ttttttcata accatcacga aaggaatttt gcaaagattt
     1021 tattccatct caaaacaatc atctcaaaaa tgcgtttctg accgccggta aaacaaaacc
     1081 ctctaagaaa atacttagag ggttttgaaa tttggctccc cgacctgggc tcgaaccagg
     1141 gacctgcgga ttaacagtcc gtcgctctac cgactgagct atcggggaat aagcgcgaat
     1201 aataaaacag aaataccgaa cgtgtcaata taaattgaca atcgattgca ctttatctgc
     1261 aagcccgcaa tctttatatg ttatatggct tccgcaatat gccttgccgc acgtttggca
     1321 tccaatagga ttaaaccaag tctgccggtt tcttttgcca ccagcaccaa cacggcatct
     1381 ttacccgcct gacttaaaag gatatagcct gattttcctt taatcatcac ttgttccaat
     1441 tccccgcagg cgagttcctg caccgagcga ctccccaaag caagcaaagt ggcagaaatc
     1501 gcccctaccc tgtccgaatt caaatgtgaa ggaagcattg tcgccatcgg caatccgtcg
     1561 gttgagataa cggcagacgc gataatatcc gtagatgtat tgtttaaatc ttcaaggatt
     1621 gaaatcaata attgctgcat aacccctcct ctctcccaag ttttacacgc ggttgctgta
     1681 acggcggtat aaaatcctta ccaaagtaac aaatgcctct ttgcccaaat cgggaatgcc
     1741 gccgataacc aaaataaatt tggttgaacc gatatacaat gggaaaaatg tcaattcgct
     1801 ctgaccggaa ggatcgcaaa cgccccaagc gttattgttg atatacaggt tgttcttaat
     1861 cagcagccgg tatttctttt ccatctgtgc gacttctgcc gccaacaacc ccaactcttc
     1921 cgccgcctca tgatggaaat tggcgttggc aagatacaga ccgttccgat cgaccaataa
     1981 cgccttaccg ctgccggaca attgttccat cagcaacggc aattgctcat ccgacaaatt
     2041 gatgccgtct gaatgaccgt tttcatcgcc atagaggaat tcgagttttt gcaaacggta
     2101 caacaggttc aaagcggtat cgatgtcggc ggtgtccgcc caggtaagca gcttctcact
     2161 gctgaccatt tcgtccgcat ccgctttcaa caggctgtgc aacaaagttt taccggcact
     2221 gggggcatcg ctggatacgg cataaaatgc accggcagga gtcaggcggg gatataaatt
     2281 tgcttgtagt gaaagtgttg attccatatt aaacctccag tcccggatca atagaaaata
     2341 acattgcgct aaccaattgt tttacgtcat cttccttacg ggcatcaatt tcaaaaaccg
     2401 gaacattaag attatgtttt gcaagatatt tgtgatacac gtcgataccg ggctgagagc
     2461 gtatatccat cttggtaata ccgacaacga cgggtgcctt ctccagcagc cctcgaaacg
     2521 aatgtaaaaa gaattccaaa tctttcaacg gattggttcg ggcattatct aaaagcaaga
     2581 ccaaacccat actgccttgg cttaagattt cccacataaa gttgaaccgt tcctgaccgg
     2641 gcgtaccata taaatggact ttggtatcct catccaagct gatggccccg tagtccatcg
     2701 ccactgtcgt attccttttc ctatccaaag tcatatcgga tgcggaagca tcggtctgaa
     2761 cgagtgcttc gtccgaaata gccgcaatgg cagtggtttt ccctacgccg acaggtcctg
     2821 tgaaaataat tttattttct ctcatctctc cgcctcttag ctgcccaaca gttttttcat
     2881 cagcctttgc agaaggccgc gcgactggga ttgggatggt gttgtgattt tttccgcttt
     2941 tttcatcatt tcactatcag aggcagaatc ggctccgata tttattttat ccgccatatc
     3001 ggagtatgcc tgttgtgaaa ccgtttttaa atctaccgac aaaaacccgg ttgtataggt
     3061 tgccgcaaga taattcagaa tatcattgag gtttaaaggc atcactttat acaacacgtt
     3121 aaggttgacg gatgccttgg tcagaaatgc cgacaagcgt atcgaccccg gcacatttgc
     3181 caaccgggtc aggtttggcc aagatttcaa cgtaaacgga gtatcgggag aaatcggata
     3241 aatcaacctg ccctgcgccg tccaaatgga aaactgccac atacaggaca taatgcctac
     3301 tttagccttt tcgcgccatt gcgggttatc gggaacagtc ttgcagctga cctgcaaatt
     3361 ttcgtctttg cacaattctt cgagtttttg cacactttct gtcagcaaaa cccgttgtat
     3421 cgaggggaaa acaataagga ccggcttatt tccatgcaag atagcgatgt cctgcctgtt
     3481 cttttccgca aaccgcaacg cccccaataa tcctttattc gggttaaact ggcgtatcgt
     3541 aaccgtacgc tgcacattcc cgttattttt cgccgaccgg tctgcgggtg caataaacga
     3601 tttcccataa acattctcgc cctgcaacaa tttgcggagc ataggaaaca atgtttcaaa
     3661 ccgaatcggt ttgggcaggt agggaacttc agaatcggga actttctccg aacagacggc
     3721 gacgggtata tccttataac gctcggcaag ctctttccaa agttcaaaac cgccctcggc
     3781 atcggtatcc gccaaaacta aatcgggcac ggcactgccg tctgaagggg atactgtttc
     3841 ataacgggtg gtattgtgca ttttgaatgc cattttgaaa acggattcct gctgcgccgt
     3901 catccccgcc aacattacgc gtactgtttt aattttcggc agttgaactt ccatttttat
     3961 tttccgtacc gttttatttt tttaatattt tgattcatac gctgcagcag ccggctcatc
     4021 agcataacga cctcttcagg aagcctgtcc gcacgttccc tcaataccct taaaaactgc
     4081 cccaacctat cccaatcttc agtacgttca taaatatcga tcaacgtaat ataaagctgg
     4141 gactcgtcgg gatatttcaa taccgcctgc tccaacacat ccattgccgc ttcaatctga
     4201 ccatacatca gcaacgactc tacttcctta accgcatcgt ctgccggaga cgaaccggtg
     4261 ttgatcaacg aagaatcttg aagcaccaaa tcccgatgtt gcgatttgaa tttctgtata
     4321 tttttcggca gatacccgtg ccccataccg atgtctttga tttgccggtc gttcggccct
     4381 ttttccaaat catcgaaaac ttcatggtaa cccaggctgt acccccaccc cagcatccgc
     4441 tctttaacct gcctgccgta gttgcccaac gtttggtaaa gtttccacaa atgtccggca
     4501 aaacggtcta tgtccgcgta ttggtaatcg agtttcaacg catcgataat caggtttgca
     4561 ggtttttcgg aagtttggat ggcacggtcg tattgtttcg atgccgtttc atagctgact
     4621 ttgtctttaa ggattttcgc accttgatcg gcacggacca aaccggcaat cgcaccgatt
     4681 tcctcttgac tgattccgga cacgtctttt ttgccccgca caatcgggat gcgcttgatt
     4741 tcttcggctt cataagcctt accgccggca tccggcgggg gcgatgcgga agctgccgtc
     4801 gcagaaccgc cttccgcacg tttttcaatc tcctgagtac cccatcccaa accttcttcc
     4861 gccaagacgc ggatacgcaa atggtttgaa tcgcgctgta atgcctgttc gatatatttt
     4921 gccaaaagtt cggaaggaat cagtttgccg tatttttgca gattgtctgc caaaacatcg
     4981 acatccccca cttcgagatt gatatcgagc agctcgcgga taaggttttc aggtttcgct
     5041 tcaccatccg gaatgccgtc cagataggca gccaaagatt cggcagcctt gccctgataa
     5101 ccgaattgct tataaacctg atactccgta agcggatcga cttcttgcgc ggatacggcg
     5161 gcggatgcgg tctcggcact ttcgttccaa gaccagtcgg gttgcgtgcc gtctgaaacc
     5221 gattgtccaa tctgatcgac ccagtccgaa tcattgcttc cgggtgtctg cccctgcgta
     5281 ttgccgacac cttgggattt gcgcttcggt gttttcttac cctgcttcgc acgcataacc
     5341 aaaagcatca gcaataccgc cagtgccaaa ccgataatta atgagttttc caaaggatac
     5401 tcccgatacc gtaacgagcg gatagctgac cgcctgcaac ggccattgtc cgcttttatt
     5461 attaacgatt tactgaaata gacttgtaag ttttaaatca taccataatt taacgtttaa
     5521 caatatgcct tctgcacaag cctcgccata ttacctttta cccacatcag tatcaatacc
     5581 cgatataaaa ataactttgc ccataagctg ccttattgcc tgcccgccgc agagtagcgc
     5641 gcgataaaaa ataacggatg ataagtcagg tgcactttgc ccgacggcat actgaacgcc
     5701 gacaagtaat cgccaataaa tccattgaga ttttttcttg tccaattttt ttggttcgtg
     5761 gctgttacgc ctgtatattt aaggtgtttg agtacatcta acggcgtatc aaagtctagt
     5821 attaccttaa aatcctcaca ccataaaagc tcaaaatctt tggctaacca agtctgccat
     5881 tgggataaag tcgggtaatt taagcctata tttgtaattt ggcggacttc ttttaaattg
     5941 tctttgccaa aggttgcaac cgccaataat ccgtttgctt tcaagcctgt tttgcaatga
     6001 gcgataaaag cgtcgggttg atgaaaccat tgcacggcag atgcgcttgc gattaagtca
     6061 aattgtcgtt gaaaaggaaa gttttccgca tcgccgcaat aaaaatcaaa ggattgcggc
     6121 agtttttcag ccagttgggg ctgcacatcg cacaaatcat taaataacca ataattcgct
     6181 gaaatctgtt tttgcagcaa ggcactcaac attcctgagc cacagcccaa ttccaacacg
     6241 ttttccaatg gcatatccgg caaataatct tgcaaatgcg ccattagatt aatcgtcatt
     6301 ttttgttgga ttaaggcgtg ccggtcataa tcgtttaatg ctttttggaa agcctgccga
     6361 atgcgcgatt tatttatggc agtcagtgat tccatagtgc cgaccaatgg gtgaatcttg
     6421 aaaacaggta atgtccgccg tcaatttccc gaacggtgca acgcgctgtc caatatcggt
     6481 gctgattggc agacataaaa attttatcgc ccgatccgac caaggcattt gtccagcgga
     6541 taaaatctgt acgcctatct tgcccgatca tcgcaaaaag tgcggtaagt tcttgatgaa
     6601 tttcgtcaaa cggacgggcg gaaaattgtt ggtaatcttc aaaagatgct ttatcgccac
     6661 acattctgcg ttcaaatttt aaacgagtat ttcccgtgag gttctccaat gtgcctttaa
     6721 aaacggcgaa agggataccg aaattatcat cgcaaggcaa acctgtgcca ttcactgccg
     6781 ttgcggattt taatcttatt ccttgcaatg ccctctctgc cgcccaaacg cccattgacc
     6841 acgccaccaa acggatgtgc cgataggcgg aaaaatcaaa atccaaattt aaatcttgat
     6901 aatcatagca aatcaataaa tcgtgatttt ccggcaaaat caaatgattt acagcatcgg
     6961 gcggcgttcc ccaacctgca aaatacagga ttaaatgttc gccttgatga ttgtaaaatt
     7021 ttgttttcat atcatatcct tacaggcacg ccgcaaactg ccgcacttca tccattgtca
     7081 tatctgccgt caaagacaat cgtattctgg atgtaccttt tggtactgtc ggtggtctga
     7141 tgggcaggca ataataaccc tgcctttgca ggtattccgc tttggcaagg gtggcttcat
     7201 tcccgcctaa aatatagggg acgatacagg tttggctcgg cattatttgc gtccgatgcg
     7261 ccacttcccg ccgtaaaaat gcacttaact gctcaagatg gcttctttct tttgagaatt
     7321 gcggcaatcg ttcaaaaata aaataagtcc aagccacatt aaacggcggc aatgcggttg
     7381 aaaaaatcaa tgggcgcatt tgattaatca aacattcttt caatacttgg ttgcagacgg
     7441 catacgcccc caccgaggct aaggctttac cgaaagtgcc aaccaataaa tcaatctcgg
     7501 caatcaaatt atcccgttcg gcaatcccca atccgttttg cccataaaca ccgattgcgt
     7561 gagcttcatc cacataaaga taagtattgg gaaactgttt ttttaattgg acaagctgtt
     7621 tcaaatccgc cacatcgccg tccatactga aaacagattc ggtaacgata aaagtgcggt
     7681 caaattttcc gacgtttttt tcaagcagat ttttcaaatg ttcataatca ttatgacgat
     7741 aacggaaaaa cgcacaccgg ctcaaacgga tgccgtcaat catactggcg tgaacaaatt
     7801 tatctgccaa aatcaaactt ttcgtcgtcg tcaaagcagg caaaataccg agattggcgt
     7861 gatagccgct gttgaacaat aacgcgcttt cccgttggaa acgttgtgcg acaagctctt
     7921 ccaaatcggt ataaatagga aagttgcccg ttaataaacg cgatgaagaa ctggtaaaag
     7981 agggaaaatt accgccgtat tgctgcaaaa aagaccggcg caagttttca tctgatgcca
     8041 aacccaaata atcattagac gacatattca gcattttgca gttttcccgc gtaatatacc
     8101 gcccttgatg aatcaaatcc ggaatcgaac gatattggtt ttgcgcgccg agttgttcaa
     8161 gctgttgttt aaaaaccttc attatgatgt aaatattcct gagttaaagc ctgaaccaac
     8221 ccagcatcaa acaatcaagt aatgcccttt taataaaaac cgttttgccg ccgacctttg
     8281 ccacacccct gccttcataa tccaaggcgg aaatttccgc gacatttgtt tccgttgcca
     8341 ttacccaatc cgtccgcact gtaccgaagc gcatattttc gcacaaattg gtacggtttc
     8401 aggtatgatt tgccgaaaac ctatcggaca cacaccatat tttgatatga ataaaacaat
     8461 ctgccgcact gctgcactat tgataagcgg tttttcatac gcaaacaccg tcattcccga
     8521 tgtctccccc gtcgcacaag gtcaacatgt cttcattaac atccctcagc aacgcctgtt
     8581 cctctacacc gacggcaaac tgaccaaggt ttatcccgtt gcagtaggtc gggcgatgac
     8641 gcaaaccaat ctaggcgaac ataaaatcgg tgcgaaagcc tataaccctg tttggtacat
     8701 ccccaaatcc atacagaaag aacgcggcga cggcgtaaaa accatagccg ccggcccgga
     8761 caaccctttg gggccggttt ttgtccgctt aggcgacccc aaactcggtt tgggcataca
     8821 cgggaccaat gcgccggcca gcgtccccgg ggttcgaagt cacggctgcg tccgcatgaa
     8881 atcgcccgac gcgctcgagt ttgccaaaac catcgccagc ggctcgcccg cctccgtcat
     8941 ctatcaaatg gcgggtctca atgaagatgc ggatcgcaac ctgtggcttg ccgccttccg
     9001 cgacccttac ggtaagaaca accttgacat cgcctctctg aaaaaaagta ttgcgcaatg
     9061 ggcaaaaaca cagggtaaaa ccatcgcgcc cgagaaagtc gatgctgtac tcaaagaccg
     9121 caccggatcg gccgtctgcc tgacctgcgg caaaaacggc aagatgaaga tgccgctcaa
     9181 atcgctggcg tggatacagg gttcttcctc atacagccaa gctgaagtca ttgaacaaac
     9241 tgaggaaacc aactctgccg aaatatccga gacacgcacg cccgaagtgc ctgatgtaca
     9301 cacgcccgag gcacaaccgc atttaaatac ccaatccgac ggcacgccga ctgcctatac
     9361 cgaaccggct gccgattcat cgccgcaagt agaaacacct gatcaggctg cttccgagcc
     9421 ggttgatgta ttattttcaa tagatgtgat acggcaggga aatttgcgtt taggtaattg
     9481 aataaccttc tgattattta atgcttatgt ttatcagaag ttgataggcg gtttggtttt
     9541 tgtttggctt ttcctacacc gccgcctgcc gctttctgca acattcaagc gcacaaatat
     9601 gccatctgaa ggctttagac ggcatatttc acgataatag tcgcccaatc aaataaacac
     9661 agattggaaa tttattttat ttttccctta ccaatacccc agaactttcc accaaatgct
     9721 gccgataacg aaaaaaatca gaaaattgac cacgctcatg ataaaacccg ccttccacca
     9781 ttctcccatt gtggtgtagc ccgaaccgaa aatcacaggc gaagtaccgg tcgcataatg
     9841 agtgagggtc atcataatgt tagatgcggc cgccatcatc agcgcggtcg gcatcgccgg
     9901 ggcattcagt gaaacagcag cagcgagaaa tgcgccgaac atagcggtaa tatgtgcagt
     9961 agtactggca aacatataat gcgcatacat ataagcaagc acgaggatta cgcccgcagc
    10021 cgtgccgcta acgcccaaac cgccgacact ttccgccaac actccggaga accatttaat
    10081 cagtccgagt ttatttaaaa atgcggccat cataatcaat gcgccaaacc aaataatcgt
    10141 atcccacgca cttttttctt tcaaaacatc gtcccaagtc aatacaccgg aaagcaaaag
    10201 caggcttaat ccgataaatg cggtggcggt ggcgttgata ctaaaagcgt gattgccggt
    10261 aataagggcg ggaacatctg cccacaacag caacaagata ccgaaaatga ccgccataat
    10321 gatttcgtct gccgacattt tacccatctc cctcagacgg tctttggcaa attgaacggc
    10381 attgggcgtt tctttaattt caggcggata caaaaaatat aaaatcaaag gcataacgaa
    10441 aaaggcgata acgccgggaa cagccattgc ccacgcccac gccccccaag aaagacggaa
    10501 actactgcct aaattttcgg caatcaagtt gacgattaaa gggttggggg cagttgcagt
    10561 aataaacata gccgacgaaa tgggattgga atgatagttg accaaagcca aatatttacc
    10621 catcttgcct tctgtgcctt ttgcgggatt ggagccgtaa ctgccggcaa tcgactgcat
    10681 aatcggatgt ataatgccgc cgccgcgcgc ggtattggaa ggggtaacgg gagccagcag
    10741 cagttcggaa agagcgagac tgtaaccgat gcccagcgtt tttcttccaa aaacggcgat
    10801 aaacaaatat ccgatacgca tccccagccc tgttttgagc aaaccgcgcg aaatcataac
    10861 tgcgatggca atcagccaaa tcaacggatt ggcgaacgca ctcaacgcat cgctcatcgc
    10921 cgcgcccggt ttgtcggcgg ttacgccggt tactgcgacc aacccgacgg caataatcga
    10981 cagcgcgccc aacggcatgg ccttgccgat aatggcggca atcacaccga caaacatggc
    11041 cagcagcgtc caagcctgag gcttgacccc gtcgggtacg ggcagtgcca aaaccagggc
    11101 gcacaatact gcggcaatgg cgaggggtat cggtttgaaa cccaatttca tcatattgac
    11161 ctccgtaaaa aagaccgtcc cgaaaaatcg gaaaaataat atttaactaa ttgttttata
    11221 agatatattc agatatttca ccgtctttcc gatatgcggc tccgggcaac ttttgtttca
    11281 gtatttgaat tttcattaga ctgaatacgc cgtttgaacg gcacggcgaa aacccggggg
    11341 atgccggacg ttcagtcctt tttcgcacct tgaaggtaaa gcatttccaa ggcaattgtg
    11401 gcgccggcca aagcggtaat gtcggattgg tcgtaagagg gggcaacttc tacaacatcc
    11461 ataccgacga tgtcgagatc cgtcagccca cgtaggattt ttaatgccct gtcgctgctc
    11521 aagccgccgc atacgggcgt accggtcccg ggggcgaacg acgggtcgag gcagtctatg
    11581 tcgaaagtca ggtaaacggg catattgccg acggtttctt tgattttacg gacggtctct
    11641 tcaacactgt cttcattaac ttggggggcg gtcaacacag taaaaggcaa ttttttactg
    11701 tgttcggtac gtatgccgat ttgtacggaa cgggacgggt cgatgaggcc ttccttgggg
    11761 gcggtataga acatcgtacc gtggtcgtat tcgctgccgt tgtcgtaggt gtcggtgtgc
    11821 gcgtcaaaat gaatcagtgc gagtttgccg aaatagcggg cgtgggcgcg caacaacggg
    11881 agggtaatga aatggtcgcc gcccaaactc aaacagcgtt tgccggaaga aagtaatttg
    11941 ccggcgtgcg cttccatttt ttcgacaaaa tccctgctgt cgccaaaaga aaaaaccaag
    12001 tcgccgcaat caataatgtt caggcgttcg cgcacatcaa atgtccatgg aaacctgcgg
    12061 tgctcccaag cgaggttgac ggaggcgcgc cggatggctt caggaccgaa acgcgcgccg
    12121 gaacgccctg aaaccgccat atcataaggc acgccggtaa taacccaatc ggcatgactt
    12181 tcatacggca taaaattaag cggcaggcgc aaaaacccga aattattgga aacgagggag
    12241 ttgtcggttt gtcctgccag tgtgctgtat tgcatcgtaa tgattccttg taattggttt
    12301 caatcggtcg tgatgattgg tgtttgagta agaaaatcgg gcttcagacg acatatccga
    12361 tgccttgatg cgtctattcg tcttccaaat aggtataacc attaagcccc gcttcgagtt
    12421 cttttaagaa agacatagcc tgcgaggcag gaaggtctga atgttcgatt tgttcgcgat
    12481 agcgtttcat cagctctttc ggatcttgat aaacgtattc gagcatatcg gcaacggtgt
    12541 ttccttcatc gtaatcgatg acggtaaatt gtccgtcttc ccctacaaca acatcggcag
    12601 tggcagtgtc gccgaaaaga ttgtgcatat tgccgagtat ttcctgatat gctcccacca
    12661 taaaaaagcc taaaagcggc ggctcttctt cgggataatc aggcataggc atcgtaccgg
    12721 cgatgccgtc tccgtcgatg tagtggtcaa tcgtaccgtc tgaatcgcag gtaatgtcca
    12781 acaacacggc gcggcgcgca atcggttcat tcaaaccggt aatgggacaa acagggaaaa
    12841 gttgatctat gccccaagca tcgggcaaag attggaagag tgagaaattg acatacagct
    12901 tatcggcaaa acgttcttgc aattcgtcaa taatggttcg gtgagaccgg tgtttttcat
    12961 taaacaattc gccgacttca tgacagatat ttaaatacag ttgctccgcc cacgcacgtt
    13021 gcgccaaact caacagcccg acattatact gattatgcac atcggcaaga tcaaactgcc
    13081 cttcgtgtat ccagctgcgt aaggaacgtt tttcccgcga ggcggaaata tccgtccaag
    13141 tttcccacat actgtgcaac acacgcggtg cttcgggcga tggcgcatcc agccggcgcg
    13201 gtttgtaacg ttcaacgcct ataacattag caaccaaaac ggcgtgatgt gcggtaatgc
    13261 cgcgcccgct ctcggtaatg attgtcggat gcggcagccc gtgttcgaga caagcctggc
    13321 tgatgcccca tacgactgtg gaggcatatt cgttgaggct gtaattaacg gaacaatccg
    13381 attgtgtgcg gtttccttcg taatccacgc caagcccgcc gcctacatca aaacagcgga
    13441 tatttacccc cagtttgtgc aactcaacat aaaaccgagc cgattcgtgt acacctgtgg
    13501 caacatcacg gatgttccca agctgcgagc ctaaatggaa atgcaaaagc tgcaggcagt
    13561 ccagcctgtt tttttgtttc aaaatatcga ccagttgcaa aacttgggaa gccgacaagc
    13621 cgaattttga tttttcccca cccgaagact gccattttcc cgaaccttgg gaagccagtc
    13681 tggcgcgcac acccaaacgg ggcttgatgc cgagtttttc cgcctcttcc aataccattt
    13741 gtatttcgga cagcttctca atcaccaaat aaacctgatg ccccagtttt tcgcccatca
    13801 aggcgaaacg gatatattca cggtctttat agccgttgca gacgattaat gtttgccggg
    13861 tgccggcgtg tgccaaaacc gccatcagtt cggctttaga accagcttcc aaaccatgcg
    13921 gttgtccgct tgacataagc gattcgatga cgcggcggtg ttggttgacc ttgatagggt
    13981 aaaccaaaca ataaccgccc ttatagccgc actcttcccg tgccgtctga aaggcgcggt
    14041 taatgtcgcg gaggcggtgt tcgaggattt gcggaaaaca aaacaaaaca ggcaggcgcg
    14101 cctgatgttt ttgttgcacg gcttcagtca gtttttgcag tgaaacagtt tgattgtgtt
    14161 gcgagggatt ggggcggacg atgatttcgc cggaatcgtc aacatcataa taacctatgc
    14221 cccaatgatt aatgttgcac acttcacgga tggtaaggat aggcataata aacctgctcc
    14281 gtctgtcgtg ttgaaaagga atgattataa caaatcagcg tgaaatgtca tttttttaat
    14341 aagaaaagcc tgcctcatac ctgatgagga acaggcaaaa tgccgtctga acgcttcaga
    14401 cggcattttg gttcatcttt ccatcaaagg agtcagcgat cgagctgctc tttgatgatt
    14461 ttcaggtcgg ggtaagacaa cacgatgtcg tattcgcggc cgtctttata ggcttccact
    14521 tccagcacag gcttacccca atggtcgtcg gcatcgacat cgtgaacctg ataaccgcgc
    14581 tgttccaaca ttttcacagc ttttacgcgg ttctgttcaa aattgggatc actgtaaatc
    14641 tgacgctcgg cagagtcgct ggcaaatgcg gttgcggcac tcagggaaac aacggcagcc
    14701 aataacagtt ttttcatttt cagtcctttc tttatcggtt gattgaataa gatgtgtttt
    14761 tcaatgccgc cattaaaaca cagcaaaatt aggtttgaat tagagggaag tcaagaagcg
    14821 taaatgccgt ctgaaaaaaa cagagccgtc aaacggctcc gttttcctta tgcttctttg
    14881 gtttcagccg ccttttggcg caggcgcagg ctcaattcac gcaactgctt gtcgtccacg
    14941 ctgttgggcg cgttggtcag caggcattgg gcacgttgtg ttttcgggaa ggcaatcacg
    15001 tcgcggatag attcggcgcc ggtcatcagg gtaaccaaac ggtcgaggcc gaatgcaagg
    15061 ccgccgtgag gaggcgcacc gaatttcagg ttgtccaaga ggaagccgaa tttctcttgt
    15121 tgctcttcag ggctgatttt cagcgcggca aacactttct cttgtacgtc tgcacggtgg
    15181 atacggatag agccgccgcc gatttcccag ccgttcaata ccatgtcgta ggcacgggcc
    15241 aggcagtttg cagggtcgga aaccatcagg tcttcatgac cttctttagg cgcggtaaac
    15301 ggatggtgta cggcaacgta gcggtcggct tcttcgtcgt attcgaacat tgggaaatcg
    15361 acaacccaca aaggtttcca ttcgtctgtg aagtagccgt tgtctttgcc gtgttccaag
    15421 ccgactttga tacgcaatgc gccgatggct tcgttcacaa ctttggtttt gtccgcaccg
    15481 aagaaaataa tatcgccgtt ttgtgcgccg gtacgctcga tgatttcttt cagggcattc
    15541 tcagacagga attttacgat tggagattgc aggccgctgt cttcgccgtt tgagaggttg
    15601 cccacatcgt ttactttgat gtatgccaga cctttcgcgc cgtagatgcc gacaaattta
    15661 gtgtattcgt cgatttcttt gcggctgaat tttgcgccgt tcggtacgcg cagggcgact
    15721 acacggccgc ctttcatatc tgccgcgcca cggaagactt tgaattcttc cgttttcatc
    15781 aggtcggtca actcggtaaa tttcaagttg atacgcatat ccggtttgtc agagccgtag
    15841 tagaacatgg cttcggcgta aggcatacgc gggaagtcgc ctaaatctac gtttaaagca
    15901 tctttgaaga cttgtttcgc cataccttcg gtgatgtcca tgatttcatc ctcgttcaag
    15961 aacgaggttt ccaagtcgat ttgagtgaat tcaggttgac ggtcggcacg caagtcttcg
    16021 tcacggaaac atttggtgat ttggtagtaa cggtcgaaac cagcaaccat caacagttgt
    16081 ttgaacaatt gcggagattg cggcagagcg aaaaactcgc ccggatgaac gcggctcggc
    16141 acgaggtagt cgcgcgcgcc ttcaggcgtg gagcgggtca gcattggggt ttcgatgtcg
    16201 ataaagcctt gtgcgtccaa gtagcggcga acgcccatag caacttggta acgcaggcgc
    16261 aggttgcgtt gcatggcagg acggcgcaag tcgataacgc ggttggtcag gcgaacgttt
    16321 tcgctgatat tttcatcgtc gatttggaac ggaggtgtag cggcagcgtt caagacttcg
    16381 atttctttgg caagaatctc gattttgccg gaaatcattt tgtcgttggt cgtaccttcg
    16441 ggacggttgc gtacgcggcc ggtaatgctc aaaacatatt cgttacgggc ggaatcggca
    16501 gcggcaaacg cttcgggcgt atcgggatcg atcacgactt ggacgatgcc ttcgcggtcg
    16561 cgcaggtcga taaaaatcac accgccgtgg tcgcgccggc ggtgtaccca gcctttgacg
    16621 gtaacggttt ggtctaagta ttgctcgctg atcaggccgc aatagttggt acgcataaaa
    16681 tcacctttta ttaatttaaa ctgaaaacag aaaatgccgt ctgaacggcg gctttattgt
    16741 tgttcgggca aatccgcctt ttcagacggc ataggtcctg ccaatgtttt gacgggcagg
    16801 tcgtcaggga tgaccatacc cagcgaaatc acatatttca acgcttcgtc cacgctcata
    16861 tcgagttcgc gcacatcgct tttctttacc ataatatagt aaccgccggt cgggttgggc
    16921 gtggtcggga catacacgga aagataatcg ccatcctgcg gcaatgcgac cttaaccgca
    16981 ttcgacacct gaccggacac gaatgcgatt gtccaaatac ccgattgggg aaacggcacg
    17041 agtactggtg ttttaaacga acggctgctg tcggacagca acgattcgga tacttttttc
    17101 acactcgaat agatggactt cacaaccgga atccgcccca acaagctgtc ccacgcggca
    17161 agaatctgcc ggcccaatac gtttgcggca aacaatccgg ttacaaacaa tacggcaatg
    17221 gcaacgataa cgcccagccc cgggatatta aaccccaaaa catattgcgg ccgccattgc
    17281 ttcggcagca ggttgacaag ctggtcggac gcggaaacga tataggaaac cacccaaacc
    17341 gttaccgcaa tcggcagcca gaccaaaatg cccgtaatca gatatttttt taacgccttg
    17401 gcagctttac cgccttcggc cgcaggttcc gtcatcttgc ttgattccga caaagtccgt
    17461 acaaaccgca cattatacgc gtttgcccgg attcaaacga aatttttatc ccgccccgcc
    17521 aaaccgccgg cggcttcaga cggcacggca acttgatata ccgtctgaac acgcggttca
    17581 gatgccgtcc aagtcgttga acatcaaccc gataccgata ccgttctgct tgtggttgta
    17641 gtcgatcagg ctctcgccgt aaccgtggaa tccgcgtacc acacctttga gtttgccctt
    17701 aatcggaaac gtgtaggcgg cttcaatcgc gccgtagccc gttttggggt tgtagcgcaa
    17761 tacggaatac acattctgcc tgtcgttcag gcggtactgc agcttcacgt cgccataccc
    17821 catatagtcg gcaatatcgg gattgtcgtt tttatcgccg ctctgatcga acgcacgcac
    17881 ccacacgcgc ggaatcaccg tcaatttgcc ccattccatg cctgccatgg cgtaaatcct
    17941 gttccacgaa cgcgattcgg gacggctctg tccgttggac tggtggacaa aacccgcacc
    18001 gagcatacgc agcctgccgc cgaacggcaa atccgccttc acaggctggg tcaggaaaat
    18061 ttcaggtttg taatccgtat tgcggaacgg cgcggatttc ctgccttggt tgtaaatctg
    18121 ccaatcggat ctttgggtgt agccgaacca cagatccgcg cgggttttaa acaaatcttc
    18181 ggcaattttg cttttgaacg aaacctgcaa tttggtttcc gcacgtttct gctgtccgaa
    18241 tttttcctgt acagtcgtac cgcgcgtcgg cgaacccggg gcatagttgg gcgaattgtt
    18301 gtaccagagc ggcataaggt acatcggatt gtgttcgcgt acgcccaaca gcccgcgcaa
    18361 atcgtttttg tccaagtcgt acatcaggct caaaggcgta tagatgtcgg cggtttcgcc
    18421 cgcactgtcg gcaggaagcg catccccgcc tttttcaaca acaatgaccg cctcgccctt
    18481 atccaggctg ctgcggacgg tttccgtcag attgagtacg gctttcgact cctgcccttc
    18541 ctgccctgcc gaagacggaa gctgtgccgc aaaaatcctg tcgtaacacg ccaaacgcgt
    18601 aacattgtcc gtcaaagcgg cgcattgcag cgcggtctct ccaaaagcgg ataccgtcgg
    18661 caacagtcct gtcaaaagaa tatagcgcat attccgtgta ttcatctccg cccccattgt
    18721 cggcatattg gttttcaaac ggcattttat agcggattcg gataaaaaac cgcacccttt
    18781 ccgccatttc gggatttagc ccccgtaata cagaaaaccc cgaaaccgtc gggcttcagg
    18841 gttttccgct tatcgcgtat caaccgcctt ggcggttttg caaaaatcaa gccagtgctt
    18901 ttacttttgc agacaaacgg cttttgtggc gtgccgcttt gtttttgtgg aacacgccct
    18961 tgtcggcgat gcggtcgatg actttgacgg actcttggta aaccgcttgt gcggcagctt
    19021 tatcgcctgc ttcgactgct ttcaatactt ttttcactgc agtgcggaat gcggtacgca
    19081 ggctagcgtt gtgggcgcgt tgtttgacgg actggcgggc acgtttgcgt gcttgtgcgc
    19141 tgtttgccat attggaatct cctgaaagaa aaataaattc tgtaaacgcg caattttaaa
    19201 gggggtttac attatatgca agccttttct ctctatccgc ccgtcggaca ccctgacagc
    19261 gcaaaaaaac ggcaaaatcg tgcggacgtt ttccccctct tttgaaaaca aggtaatttt
    19321 ttaggaaaat caggtcaata tcgctttgag tttgcacaaa tttactttac aatgcccaaa
    19381 ccttgcgccg gggtgtttat ggggcgcggg gttttctttt tttccaacct ctttcatcag
    19441 gaatctgccc gtatgaaaaa atccgtatta gccgttttgg ccgcgctgtc tctggcagcc
    19501 tgcggcggca gcgaaaaaaa cgccgtgcaa cctcaagcag acgctgcgtc tgctgccaat
    19561 gccgaagccg ctgccaccga tactctgaac atctacaact ggtcgaacta tgttgatgag
    19621 agtacggtcg aagactttaa aaaagccaac aatctgaagc tcacttacga cttgtacgaa
    19681 aacaatgaaa cgctggaagc caaaatgctg accggcaaat ccggttacga tttggttgtg
    19741 ccgggcatcg ccttcctacc gcgccaaatc gaggcgggcg cgtatcaaaa agtcaacaaa
    19801 gacctgattc ccaactataa aaacatcgat cccgaactct tgaaaatgct ggaaaccgcc
    19861 gacccgggca accagtatgc cgtcccctat ttctccggcg tgaacacgat tgcgattacg
    19921 gcgaaaggca aagagctttt aggcggcaag ctgcccgaaa acggctggga tttgctgttc
    19981 aaacccgaat acacccacaa gctgaaatcc tgcggcatcg ccctgtggga caccccgagc
    20041 gaaatgttcc cgattttgct gaactacttg ggcaaagacc ccaaaggttc gaatcctgaa
    20101 gatttgaagg cagcggcgga agtgttgaag tctatccgtc cggatgtcaa acgtttcagc
    20161 ccgtccatca tcgacgagct ggcgcgcggc gacatctgcc tggcggcagg caacggcggc
    20221 gatttgaact tggcgaaagc acgttccgaa gaagtgaaaa acaacgtcgg catcgaagtg
    20281 ctgaatccga aaggcatggg cttctggatt gagtcttggc tgattcccgc cgatgcgaaa
    20341 aacgtcgcca atgcccacaa atacatcaac tacacgctcg accccgaaat cgcggcgaaa
    20401 aacggcatcg ccgtaacctt cgcccccgcc agcaaaccgg cgcgcgaaaa aatgcctgcc
    20461 gagctggtga acacccgttc catcttcccg aacgagcagg atatgaaaga cggttttgtg
    20521 atgccgcaaa tgagtacgga tgcgaaaaaa ctgtctgtca gcctgtggca gaaaatcaaa
    20581 gtcggcacca actgatttga agcattaaaa atgccgtccg aacatcgttc ggacggcatt
    20641 ttatattgga ttgaaataga aatatttata tagtggatta acaaaaatca ggacaaggcg
    20701 acaaagccgc agacagtaca aatagtacga aaccgattca cttggtgctt cagcacctta
    20761 gagaatcgtt ctctttgagc taaagcgagg caacgccgta ctggtttttg ttaatccact
    20821 ataccgtcgt tcccacggtc aggatttcag attgcggaca tctgtcagaa aagacaaaaa
    20881 accttccgcc gtcattcccc acaggcgaga atccgatccg ttgaaattcg gttgttttaa
    20941 ataaattctt gcagctttga ttttctgttt ttccgataac gccgtaactt tgaaacgcga
    21001 aagcggtaat ccgatccgtt gggattttgc aacttcaaat caatccgcaa actgaaatcc
    21061 cgtcattccc acgcagtcgt gaatccgaac gcgtccgcac ggaaacctgc atcccgtcat
    21121 tcccacgaaa gtgggaatcc agttttttga gtttcagtca ttcccgataa attgccttag
    21181 cattgaatgt ctagattccc gcctgcgcgg gaatgacggg atttgagatt gcggcattta
    21241 tcgggagcaa cagaagccgc tctgccgtca ttcccacgaa agtgggaatc cagttttttg
    21301 agtttcagtc attcccgata aattgtctta gcattgaatg tctagattcc cgcctgcgcg
    21361 ggaatgacgg cggagcggtt gctgtttttt c
//
LOCUS       DAWFIE010000029        21375 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWFIE010000029 DAWFIE010000000
VERSION     DAWFIE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21375
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.029"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(311..1753)
                     /gene="aldA"
                     /locus_tag="WGQ71_001007"
     CDS             complement(311..1753)
                     /gene="aldA"
                     /locus_tag="WGQ71_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ0707032.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGTEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1861..2778)
                     /locus_tag="WGQ71_001008"
     CDS             complement(1861..2778)
                     /locus_tag="WGQ71_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0707033.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRRRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWNLSPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAGADAMPSEDFSRTLNAAFAGGA
                     HQVNRLTDALNISERTLQRRMRDHFGITTSEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            2951..3751
                     /locus_tag="WGQ71_001009"
     CDS             2951..3751
                     /locus_tag="WGQ71_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0707034.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSVMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLHQDLLG"
     gene            3799..4575
                     /gene="mlaE"
                     /locus_tag="WGQ71_001010"
     CDS             3799..4575
                     /gene="mlaE"
                     /locus_tag="WGQ71_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ0707035.1"
                     /translation="MNFIRSVGARTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            4626..5120
                     /gene="mlaD"
                     /locus_tag="WGQ71_001011"
     CDS             4626..5120
                     /gene="mlaD"
                     /locus_tag="WGQ71_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ0707036.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVGFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5157..5747
                     /locus_tag="WGQ71_001012"
     CDS             5157..5747
                     /locus_tag="WGQ71_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ0707037.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            5804..6082
                     /locus_tag="WGQ71_001013"
     CDS             5804..6082
                     /locus_tag="WGQ71_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ0707038.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTAAAFTRFRQQCRLKDTRAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6079..6906
                     /locus_tag="WGQ71_001014"
     CDS             6079..6906
                     /locus_tag="WGQ71_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ0707039.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7031..7393
                     /locus_tag="WGQ71_001015"
     CDS             7031..7393
                     /locus_tag="WGQ71_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ0707040.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7390..7776
                     /locus_tag="WGQ71_001016"
     CDS             7390..7776
                     /locus_tag="WGQ71_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0707041.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            7773..8261
                     /locus_tag="WGQ71_001017"
     CDS             7773..8261
                     /locus_tag="WGQ71_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0707042.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8342..8856)
                     /locus_tag="WGQ71_001018"
                     /pseudo
     CDS             complement(8342..8856)
                     /locus_tag="WGQ71_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            8866..8969
                     /locus_tag="WGQ71_001019"
                     /pseudo
     CDS             8866..8969
                     /locus_tag="WGQ71_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            9094..9309
                     /gene="rpmE"
                     /locus_tag="WGQ71_001020"
     CDS             9094..9309
                     /gene="rpmE"
                     /locus_tag="WGQ71_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ0707043.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
     gene            9625..10254
                     /locus_tag="WGQ71_001021"
     CDS             9625..10254
                     /locus_tag="WGQ71_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ0707044.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNSANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
     gene            10400..12076
                     /locus_tag="WGQ71_001022"
     CDS             10400..12076
                     /locus_tag="WGQ71_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ0707045.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGHADFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDYHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNVDAPQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            12178..12783
                     /locus_tag="WGQ71_001023"
     CDS             12178..12783
                     /locus_tag="WGQ71_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0707046.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            12855..13247
                     /locus_tag="WGQ71_001024"
     CDS             12855..13247
                     /locus_tag="WGQ71_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ0707047.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(13276..13425)
                     /locus_tag="WGQ71_001025"
     CDS             complement(13276..13425)
                     /locus_tag="WGQ71_001025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707048.1"
                     /translation="MPSEARIAGSDGIFNVPISNRQGQHGFDTPKRVLPINGFRLRPI
                     GQNAV"
     gene            complement(13446..13892)
                     /locus_tag="WGQ71_001026"
     CDS             complement(13446..13892)
                     /locus_tag="WGQ71_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ0707049.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTE
                     VEAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(14024..14236)
                     /locus_tag="WGQ71_001027"
     CDS             complement(14024..14236)
                     /locus_tag="WGQ71_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ0707050.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(14525..16375)
                     /locus_tag="WGQ71_001028"
     CDS             complement(14525..16375)
                     /locus_tag="WGQ71_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0707051.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADANSVLRMAEDGAIKRALVLFQNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            16752..17489
                     /locus_tag="WGQ71_001029"
     CDS             16752..17489
                     /locus_tag="WGQ71_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0707052.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALASKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            17491..18177
                     /locus_tag="WGQ71_001030"
     CDS             17491..18177
                     /locus_tag="WGQ71_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0707053.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPIA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            18379..19242
                     /locus_tag="WGQ71_001031"
     CDS             18379..19242
                     /locus_tag="WGQ71_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707054.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKE
                     IVFGTTVGDFGDMVKEQIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(19304..19873)
                     /locus_tag="WGQ71_001032"
     CDS             complement(19304..19873)
                     /locus_tag="WGQ71_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ0707055.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVVHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            20031..20891
                     /locus_tag="WGQ71_001033"
     CDS             20031..20891
                     /locus_tag="WGQ71_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ0707056.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            21026..21364
                     /locus_tag="WGQ71_001034"
     CDS             21026..21364
                     /locus_tag="WGQ71_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707057.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
BASE COUNT         4765 a         6044 c         5578 g         4988 t
ORIGIN      
        1 tcgggcattc cttaaatcac ccgtgtatcg ctgtaaatct tagagatggc ggaatatagc
       61 ggattaacaa aaaccggtac ggcgttgcct cgacttagct caaagaaacg attctctaag
      121 gtgctcaagc accgagtgaa tcggttccgt actatttgta ctgtctgcgg cttcgtttaa
      181 atttaatccg ctatacaaag acaaaaacat cgatgtcggt catgactgat tgctggtgaa
      241 actgtaaaaa tgccgtctga acctgaaaac gggttcagac ggcattttct atcggggttt
      301 taaagcggca ttaaatgtcg gtttccagat aaacgacttg ggtttgcaga tattcttcca
      361 aaccgtgttt gccgtccgcg ccgccgatac cggatttttt ccaaccggcg tggaaaccct
      421 gcatcgcttc aaagttttcg cggttgatgt aggtttcgcc gaattgcagg cggcgggtaa
      481 cgtagaaggc ttcgtttaaa ttagtcgtat aaacagaact ggtcagacca aactcgcaat
      541 cgttagccag tgcaatcact tggtcgagcg tgtcgaaagc ggaaacgggc agaacggggc
      601 cgaaggtttc ttctttcata atgtccatac tgttgtcggt gtcggtcagc agggtaggct
      661 cgaagaaata gccgcgtcct tcggcgcgtt tgccgccgca aaccagtttc gcaccttgtt
      721 tgactgcccg ttccactttt tcggcaacgg ctttgacggc gcgctcttca atcagcgggc
      781 ccatttccag cgcgccggct tcggcttcgg cagggttgcc gtagcgcacg cctttcatcg
      841 cggcggtcat tttttcgata aacgcgtctt tcaggctgct gtggacatag acgcgctcgg
      901 cgcagttgca gatttgaccg gtgttgccga cgcgcgaagc caagatggat tttaccgcca
      961 aatccaaatc cgcgtctttc aacacgatgg caggggcttt gccgccgagt tccagcgaaa
     1021 ctttggtgat gttggcggaa gcggcttcca tcacttggcg gcctgcttcg acggagccgg
     1081 tcaggctgac catatcgact tgcggatggg cggacaaggc attgccgatt tccgtgccgg
     1141 ggccgttcac cacgttgaac acgcctgcgg gcaatccgac cgcatcgacg atttcggcga
     1201 aaatgtggca gttgatcggg gttacgctgc tgggtttgac aacgatggtg ttgcccgtta
     1261 ccaaagcggg acccattttg cgggcaatca ggaagaaggg gaagttccac ggcaaaatgc
     1321 cggcgattac gcccagcgga cgtttgaaca ataaaatgtt ttcgcgcggg cggtcgcttt
     1381 ggatgatttc gccttcgtaa cggcgcgccc attcggcttg gtaatcgaga tagtcggcgg
     1441 tgaacatcac ttctacgcgc gccaagtctt tggttttgcc accttcggca acgatggtgt
     1501 cggtcagctc gtcggcacgt tcgcgtatgc cttgggcgat tttgcgcaaa tacgcgccgc
     1561 gttcgaccgc aggcagacgt tcccaagccg gttgcgccgc acgcgccgcc gctacggcgc
     1621 ggtcaacgtc tgctttgccg cctttgggtt cgcgggcgat ggtttcttcg gtggatgggt
     1681 tcaatacgtc gcgccattcg ccgttgaagt cgttttcaaa gcgaccgttg atgtacatgg
     1741 ccaattgttt catttcgggt tctccagttt tgtagtcaga tgtagtttta gtttattccc
     1801 aaataaattg ccgtacaagt ttctttacac gcggatttcg ggtttgaaat caaatgcggc
     1861 ttaccggttt tcttttttgg tttccgccgg cgtgctgccg aaatattgcc tgaatgcctg
     1921 agtaaagctg gaaacgtggc ggtagccgca taaatatgcg gtttcgccta tgcttttccc
     1981 cccgttttgc aacaggtaga gcgcgtgctg catttgtttg tggtgcagcc actcgcttgt
     2041 ggtaatgccg aaatggtcgc gcatacggcg ttgcagcgtc ctttcgctga tgttcagcgc
     2101 gtctgtcagc cggttgactt ggtgtgcgcc gccggcaaac gcggcattca gggtgcggct
     2161 gaagtcttca gacggcatag cgtctgctcc cgccgtttgc cccgccgccg gcccgatgcc
     2221 gtctgaaacc gtgtcccaca agtccgacag cagccgcaaa acgtccgcct cgcggcgcaa
     2281 tgtttcgccc aaatgcccct ttgggacagc tttcaggcag gatgccgcca agccgcgcag
     2341 gtttggggac aaattccata ttctgaccgg ttcgcggtaa agcaggggtg cgaaacgcgc
     2401 atactccgga cgcagcagcc attgttccat acctttaatg gtcattttga ccgttttgcc
     2461 gcctcggtaa agatagcggc tgaacaggac ctcttccccg acagcaatta ggacgatctt
     2521 gccgccatcc gcatcgattt ggaagcggcg gcggttgatg ccgaagtcca aactgccttc
     2581 cagcaagagc acgaacgaca cataaggttc ggcgaggcgg ctgctgcaaa aatcgcaccg
     2641 tgccgttacc gtgccgccgt ggatggaaat gccgttggac aaagtatcga aacggtagct
     2701 gccctccaca tagctgccgc cgccgctttt gaccagattg acgaaccggc tgctgttcaa
     2761 atgttctgct ttttccatag ttttctgata aaggttatca tttgaaagat aacatttttc
     2821 gccacagcaa acaatccgcc gccgacaacg gcattttcac aacatttacc gtccgtatgc
     2881 cgtctgaaaa ttgcccgtct tcccgataag ccttaaaata cacgccgtca aacctaccgt
     2941 ccgtccgtac atgagcccat ccccctttat cgaaatgaaa gacgtcgcct tcgcgtatgg
     3001 cgaccgcccg attctgaaga acatcaattt cagcattccg caaggcaatt ttgctgccgt
     3061 aatgggcggt tcgggcagcg gcaaaaccac gctgatgcga ctgattacag gacagattcg
     3121 tccgcagtcc gggcaggttt tgattgaagg acgggatttg gcgggctttt cggctgacga
     3181 gttgtatgaa caccgccgcc gtatgggcgt attgttccaa cacggcgcgc tgtttactga
     3241 tttgtcggta ttcgacaata tcgcttttcc gatgcgcgaa ctgacgcgcc tgccggaagc
     3301 ggtgattcgg gatttggttt tgttgaaatt gaacgcggtc ggtctgcgcg gtgtggaaaa
     3361 cctgatgccg tccgagttgt ccggcgggat gtcgcgccgc gtcgcgcttg cccgcacgat
     3421 tgcgctcgac cctgaaatta tgttgtacga cgaaccgttt accggcctcg atccgatttc
     3481 cttgggcgtg attgcccact tgatcagccg cgtcaataag gctttgcgtt cgaccagcgt
     3541 tatggtaacg cacgacattg aaaaatcttt ggaaatcgtc gatcaggtga tttttttggc
     3601 gcacggcgaa attatgttct ccggctcgcc gcaggaaatg cgcgaactgg attcgccttg
     3661 ggtgcgccag tttgtcggcg ggctggcaga cggccccgtg gcataccgct atccggcgca
     3721 aacctcattg catcaggatt tgctcgggta aatgagccgg tgccgtctga aaccgttgaa
     3781 ccttacacat agaaatttat gaattttatc cgttccgtcg gggcgagaac cctcggcctt
     3841 attcaatccc tcggcagtat cacgctgttt ctgctgaaca ttctggcgaa gtccggtacg
     3901 gctttcgtcc gtccgcgcct gagcgtgcgc caagtgtatt ttgccggcgt gctgtcggtg
     3961 ttgattgttg ccgtttcagg gctgtttgtc ggcatggtct tgggtttgca gggctatacg
     4021 cagttgtcga aattcaaatc cgccgatatt ttgggctata tggtcgcggc ttcgctgttg
     4081 cgcgaactgg gtccggtgtt ggcggcgatt ctgtttgcca gcagcgcggg cggtgcgatg
     4141 accagcgaaa tcggtttgat gaaaacgacc gaacagctcg aagcgatgaa cgtgatggcg
     4201 gtaaaccccg tcgcccgagt ggttgcgccg cgcttttggg cgggcgtgtt ttccatgccg
     4261 cttttggctt cgattttcaa cgtggcgggt attttcggcg cgtatttggt cggtgtaacc
     4321 tggctgggct tggacagcgg cattttctgg tcgcaaatgc agaacaacat cacgatacac
     4381 tacgatgtaa tcaacggttt gatcaaatcc gccgcgttcg gcgtggcggt aacgctgatt
     4441 gccgtgcatc agggcttcca ctgcgtcccg acttcggaag gcattttgcg cgccagcacg
     4501 cgcacggtgg tttcgtccgc cctgacgatt ttggcggtcg attttatatt gaccgcgtgg
     4561 atgtttacag attgacaggc cgtctgaaga cgaaacgcgg aacatcggat attcaaggaa
     4621 ctttaatgaa aaagaacata ttggaatttt gggtcggact gttcgtcctg attggcgcgg
     4681 cggcggtcgg ctttctcgct ttccgcgtgg cgggcggtgc ggcgttcggc ggttcggaca
     4741 aaacttacgc cgtttatgcc gatttcggcg acatcggcgg tttgaaggtc aatgcccccg
     4801 tcaaatccgc aggcgtattg gtcgggcgcg tcggcgctat cggacttgac ccgaaatcct
     4861 atcaggcgag ggtgcgcctc gatttggacg gcaagtatca gttcagcagc gacgtttccg
     4921 cgcaaatcct gacttcggga cttttgggcg agcagtacat cgggctgcag cagggcggcg
     4981 acacggaaaa ccttgctgcc ggcgacacca tctccgtaac cagttctgca atggttctgg
     5041 aaaaccttat cggcaaattc atgacgagtt ttgccgagaa aaatgccgac ggcggcaatg
     5101 cggaaaaagc cgccgaataa acgtatgcca tcatattcaa actaggacac ttaattatga
     5161 aaaaatcctc cctcatcagc gcattgggca tcggtatttt gagcatcggc atggcatttg
     5221 ccgctcctgc cgacgcggta aaccaaatcc gtcaaaacgc cactcaagta ttgagcatct
     5281 taaaaagcgg tgatgccaac accgcccgcc aaaaagccga agcctatgcg attccctatt
     5341 tcgatttcca acgtatgacc gcattggcgg tcggcaaccc ttggcgcacc gcgtccgacg
     5401 cgcaaaaaca agcgttggcc aaagaatttc aaaccctgct gatccgcacc tattccggca
     5461 cgatgctgaa attaaaaaac gccaacgtca acgtcaaaga caatcccatc gtcaataaag
     5521 gcggcaaaga aatcatcgtc cgcgccgaag tcggcgtacc cgggcaaaaa cccgtcaaca
     5581 tggacttcac cacctaccaa agcggtggta aataccgtac ctacaacgtc gccatcgaag
     5641 gcgcgagcct ggttaccgtg taccgcaacc aattcggcga aatcatcaaa gcgaaaggcg
     5701 tggacggact gattgccgag ttgaaggcta aaaacggcag caagtaatcc ataagccatg
     5761 ccgccagact gaagatggca tggatttttt cctaccgaac actatgcata cagaactcaa
     5821 caacggaaca ctgcacatca gcggcgacgt aaccgtcaaa accctgaccg ccgccgcctt
     5881 tacccgcttc agacagcaat gtcgtctgaa agacacccgc gcggttgatt tgagcggcgt
     5941 cggacgcgcc gattccgcct gcgtgtcgct gctgctcgaa gtgctgcgcg gttgcaaagg
     6001 cagcgtcagg ctgaccggca ttcccgaatc cgtgcgcgcg ctgtccgaac tgtacgaaat
     6061 caaagactgg ctgaaatcat gaaaaaaacc gcctatgcct tcctcctgct gatcgggttc
     6121 gcttccgccc ctgcatttgc cgaaacccgc cccgccgacc cttatgaagg ctacaaccgc
     6181 gccgttttca aattcaacga ccaagccgac cgctacattt tcgcccctgc cgcgcgcggc
     6241 taccgcaaag ttgcgccgaa acccgtccgc gccggcgtgt ccaatttttt taacaacctg
     6301 tgcgacgtgg tcagcttcgg cagcaatatc ttgcgcttgg acatcaaacg cgcaagcgaa
     6361 gaccttgtcc gcgtcggcat caacaccact ttcggtttgg gcgggcttat cgacatcgcc
     6421 ggcgcgggcg gcattcccga caataaaaac accttgggcg acacgtttgc ttcgtgggga
     6481 tggaaaaaca gcaattattt cgtgttgccc gtcttagggc cgtccaccgt ccgcgacgcg
     6541 ctcggcacgg gtattacctc cgtttattcg cccaagaata tcgtcttccg cacccctgtc
     6601 ggacgctggg gcacgactgc cgtatccgcc gtcagtacgc gcgaaggtct gctcgatttg
     6661 accgacagtc tggacgaagc cgccatcgac aaatacagct acacgcgcga cctctatatg
     6721 aaagtccgtg cgcggcagac cggtgcaaca cctgccgaag gtacggaaga taacatcgac
     6781 atcgacgaat tggtcgaaag tgccgaaacc ggcgcggcgg aaactgccgt tcaagaagat
     6841 tccgtatccg aaacacaggc agaagcagca ggggaagccg aaacgcaacc tggaacacaa
     6901 ccttaaagcc atgccgtctg aaaatccgtg ttcggacggc attcccttct gtcggaacgg
     6961 acagtccgaa accacagatt gggctataat ctcttttttg cgcataccgc gcgacagcaa
     7021 aggaaaaacc atgtacgaag tcaaccgcag cgtattcgtc ctcatccctt tagaaccttt
     7081 ttggaactgg ctgcaaaccc tgccgggcaa ccacctcgac ggactgactc ttgaagacat
     7141 ccaagccgac gccaattcct accttgtccg cccgtgcgaa accgccgacg aagtatggga
     7201 cgaaatcgaa gcccgttttg aagacatttt cgccgccgaa cttgccgact ggtgcgaaga
     7261 cgaacgcgag tggcctgcgc tcgatgccga cattttcaac gaatggttcg acatccagct
     7321 ttctaccgtc atcaccgacc tcgaacacga accgcttgcc cgcgaagcct tccaacccat
     7381 caacctgaac tgatgaagct gaccgtccgc aactaccatc tcgacggcta cggccatgtc
     7441 aacaatgcgc gctacctcga atttttcgaa gaagcgcgct gggcgttttt tgaagaacgc
     7501 ggactgctgc acgagttggc aggcctcata ctgattgtcg cccgcatcga catccgatac
     7561 agccgccccg ccgtcgaagg cgacgtattg cagttttcat gccgcctgaa aacccccggc
     7621 acgcgccgta ttgtgctgac ccagacgatt acactgccaa acggcaaaac cgccgcagaa
     7681 gccgacatta ccctgatgcc cgtccacgcc gccacacaac gcaccgtcag cctgcccgcc
     7741 acccttgccc gcgcattgga agccttgtcc gaatgaaaaa aatactcacc gccgccgtcg
     7801 tcgcactgat cggcatcctc cttgccatcg tcctcatccc cgacagcaaa accgcgcccg
     7861 ccttctccct gcccgacctg cacggaaaaa ccgtttccaa cgccgacctg caaggcaaag
     7921 ttaccctgat taatttttgg tttccctcct gtccgggttg tgtgagcgaa atgcccaaaa
     7981 tcattaaaac ggcaaatgac tataaaaaca aaaacttcca agtacttgcc gtcgcccagc
     8041 ccatcgatcc gatagaaagc gtccgccaat atgtcaaaga ctacggtttg ccgtttaccg
     8101 tcatgtatga tgcggacaaa gctgtcggac aggcgttcgg cacacaggtt tatccgactt
     8161 ccgtccttat cggcaaaaaa ggcgaaatcc tcaaaactta tgtcggcgaa cccgatttcg
     8221 gcaaactcta ccaagaaatc gataccgcgc tggcgcaata gcgatgccgt ctgaagcgca
     8281 aaaaacagaa acgatgttcc tgttttttca gatcatccgc cccgacacgt tcagacggca
     8341 ttcaaccttt cggcagattc tttttcacca ctgccggatt gcctgccgcc aaagagtagg
     8401 gcggaatgtc ttttgtaacc accgcgcctg cgcccacgac cgaaccgcgt ccgacggtta
     8461 cgcctgccat tacaatcacc ctgcgccccg gccagacatc gtcctccaac gtaatcggac
     8521 ggatttccgt gtagccctca aagcgttttt tgaacggtca aacttgtggt tatttgaata
     8581 aaacagacat tccggctcca tcatcacatt tttgccgacc accggcccac ggcagatttc
     8641 acagtttgcc ccgatgcccg agccgtcgcc caaaaccgta tccggaaaca catacgcccc
     8701 gcgttcgata ttgaccccgc gtccgatatg cggagaaacc cgccgcgcca aaaaaccgcg
     8761 cacccgtctg ccgacgatgc cgatgccgcg catatgtgac ggcggcaacc ccgaaccggc
     8821 aaaacgcagc agcaggaaaa agaattttga tcccatcaaa cctccctcaa aagaactttt
     8881 tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta cagatagtac
     8941 ggcaaggcga ggcaacgctg tactggtttt tgttaatcca ctatacattt tatcgaatat
     9001 cctcttgacg aaacaacaga aaatagcgag aatgccgagc ttgtctgaag cataggtaca
     9061 tcccctatgc ccgctttttt gaaaggaaat caaatgaaac aaggtattca cccgaactac
     9121 cacgaagtca acgttacctg ctcttgcggc aacaaattcg caaccaaatc cgcgatggaa
     9181 aaagaaaact tcaatatcga ggtttgctcc ctgtgccacc cgttctatac cggcacccaa
     9241 aaaatcgtcg ataccaccgg ccgcgtggac aaattcaaca acaaattcgg caacctgttc
     9301 aaacgctgat tgccgctttg caagaattaa gccctgccat catttcgatg gcagggcttt
     9361 ttaatatttg gcacactgac ttcttatcta tatagtggat taacaaaaat caggacaagg
     9421 cgacgaagcc gcagacagta cagatagtac ggaaccgatt cactcggtgc ttgagcacct
     9481 tagagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttaatcca
     9541 ctatattttg atggggttga agttgacatc tacaaggttt aatgtttgaa tacattaaaa
     9601 aaatatattt gaatgagatg ttttatgatt caaaatgttg ttacttcaat aatcctgtat
     9661 tctgggacag ccgtagactt acttattatc ctaatgttat tttttgccaa aagaaaaagc
     9721 agaaaagaca tcattaacat ctatttagga caatttctag gctctgttag cctgatattg
     9781 ctaagtttgc tttttgcatt tgtcttagat tatattccta gtaaagagat tttaggtttg
     9841 ctcggcttga ttccaatttt cctaggcctc aaagttttgc tgttaggaga ttccgatgga
     9901 gaggctattg ccaaagaggg tttgcgcaaa gataataaaa acctgatttt tctagtcgct
     9961 atgattactt ttgcaagttg tggtgctgac aatattggtg tctttgtccc atattttact
    10021 accttaaatt cagcgaattt gatagtggct ttacttacct ttctagtcat gatttatctc
    10081 ttggtttttt ctgcccaaaa attagcacaa gtcccttctg ttggagaaac tttggaaaaa
    10141 tatagcagat ggtttgttgc tgttgtttat ttaggattgg gggtatatat cctgattgaa
    10201 aacaacagtt ttgatatgct atggactgtg tcgggccagg aaaaaatatt atgaaaaaga
    10261 tagtatctgc aaagactgcc atgtgcagtc ttttttgttg ccggtctttt tgtgtctgat
    10321 gccgtctgaa gcagtccctg cacgaccttt gtgcgaatat ttgctacact tggcaatccg
    10381 tccatttctt ctcacgctta tgctgaccta taccccgccc gatgcccgcc cgcccgccaa
    10441 aacccacgaa aagccgtggc tgctgctgtt gatggcgttt gcctggttgt ggcccggcgt
    10501 gttttcccac gatttgtgga atcctgccga acctgccgtc tataccgccg tcgaagcact
    10561 ggcaggcagc cccaccccct tggttgccca tctgttcggg catgccgatt tcggcatacc
    10621 acccgtgtat ctttgggttg ccgccgcgtt caaacatttg ctgtcgccgt gggcagccga
    10681 cccgtatgat gccgcacgct ttgcaggcgt gtttttcgcc gttgtcgggc tgacttcctg
    10741 cggctttgct ggcttcaact ttttgggcag acaccacggg cgcagcgtcg tcctgattct
    10801 catcggctgt atcgggctga ttccgaccgt acactttctc aaccccgctg ccgccgcctt
    10861 tgccgccgcc ggactggtgc tgcacggtta ttctttggct cgccggcgcg tgattgccgc
    10921 ctcttttctg ctcggtacgg gctggacgct gatgtcgttg gcagcagctt atccggcagc
    10981 atttgccctg atgctgccct tgcccgtact gatgtttttc cgtccgtggc aaagcaggcg
    11041 tttgatgttg acggcagtcg cctcacttgc ctttgccctg ccgcttatga ccgtttaccc
    11101 gctgctcttg gcaaaaacgc agcccgcgct gttcgcgcaa tggctcgact atcacgtttt
    11161 cggtacgttc ggcggcgtgc ggcacgttca gacggcattc agtttgtttt actatctgaa
    11221 aaacctgctt tggtttgcat tgcccgcgct gccgctggcg gtttggacgg tttgccgcac
    11281 gcgcctgttt tcgaccgact gggggatttt gggcgtcgtc tggatgcttg ccgttttggt
    11341 gctgcttgcc gtcaatccgc agcgttttca ggataacctc gtctggctgc ttccgccgct
    11401 tgccctgttc ggcgcggcgc aactggacag cctgaggcgc ggcgcggcgg cgtttgtcaa
    11461 ctggttcggc attatggcgt tcggactgtt tgccgtgttc ctgtggacgg gctttttcgc
    11521 catgaattac ggctggcccg ccaagcttgc cgaacgcgcc gcctacttca gcccgtatta
    11581 cgttcccgac atcgatccca ttccgatggc ggttgccgta ctgttcacac ccttatggct
    11641 gtgggcgatt acccgcaaaa acatacgcgg caggcaggcg gttaccaact gggcggcagg
    11701 cgttaccctg acctgggctt tgctgatgac gctgttcctg ccgtggctgg acgcggcgaa
    11761 aagccacgcg ccggtcgtcc ggagtatgga ggcatcgctt tccccggaat tgaaacggga
    11821 gctttcagac ggcatcgagt gtatcggcat aggcggcggc gacctgcaca cgcggattgt
    11881 ttggacgcag tacggcacat tgccgcaccg cgtcggcgat gtacaatgcc gctaccgtat
    11941 cgtccgcctg cctcaaaacg tggatgcgcc gcaaggctgg cagacggttt ggcagggtgc
    12001 gcgcccgcgc aacaaagaca gtaagttcgc actgatacgg aaaatcgggg aaaatatatt
    12061 aaaaacaaca gattgagccg aatttctgga ttaagtgccg gaaatcgcgt ataattgcgc
    12121 gattaaacct ttataaatca gccgttttgt aggcatcaca caggagcgac aattattatg
    12181 atgaccctct attccggcat tacctgcccc ttcagccacc gctgccgctt cgttttgtac
    12241 gaaaaaggca tggattttga aatcaaagac gtcgatattt acaacaagcc cgaagacctc
    12301 gccgtcatga acccgtataa ccaagttccc gtgctggtcg agcgcgattt ggtgctgcac
    12361 gagtccaaca tcatcaacga atacatcgac gaacgcttcc cccatccgca gcttatgccc
    12421 ggtgatcccg ttatgcgcgg caggggccgg ctggtgctgt accgtatgga aaaagaattg
    12481 ttcaaccacg tccaagtttt ggaaaacccc gccgccacca acaaggaaca ggcaaaagcg
    12541 cgcgaagcca tcggcaacgg tctgaccatg cttgcccctt cgttcagcaa aagcaaatac
    12601 atcctcggcg aagatttttc tatgattgat gtcgcccttg ctccgctgct gtggcggctc
    12661 gaccactacg atgtcaaact gggcaaaagt gccgcgccgc tgctcaaata cgccgagcgc
    12721 atcttccagc gcgaagcctt tatcgaagca ctgacacccg ccgaaaaagc catgcgcaaa
    12781 taagtccgaa atgcttgcaa aacccaccgt tttgcaggca ttttcctatt ttggcgtaca
    12841 acacggaacc cattatgccc acttccacca aaccctacat cctccgcgca ctttgcgaat
    12901 ggtgcagcga taacagcctc acgccgcaca ttcttgtctg ggtcaacgaa cacacgcgtg
    12961 tccccatgca gtacgtccgc gacaacgaaa ttatgctcaa catcggcgcg accgccacgc
    13021 aaaaccttca aatcgacaac gattggatca gcttttccgc ccgcttcggc ggacaggcgc
    13081 acgacatatg gatacctgtc ggacacgtcc tcagcctttt cgcacgggag accggcgaag
    13141 gtatggggtt tgaattggaa gcgtatcgcc ccgacacgcc gcctgaaaac acctctgccg
    13201 aaaccgcgcc ccggcccgcc aaaaaaggct tgaaattggt caaataaatc tatgccgtct
    13261 gaacggaatc gtgtttcaga cggcattttg tccgatgggg cgcaaacgga atccgtttat
    13321 cggcaaaacc cgtttcggtg tatcgaaacc gtgttgcccc tgccgattcg atattgggac
    13381 attgaaaatg ccgtctgaac ctgcgatacg ggcttcagac ggcattttgt ccgatattcg
    13441 ggcaatcagg cggtcagcac ggctttcagg attttgttga cttcgcccat atcggctttg
    13501 ccggcaagtc gggttttcaa tacgcccatc actttgccca tatccgccat acctgccgct
    13561 ccggtttcgg caacggcggc ttcgacctcg gtacggattt cgccggcaga gagcatttgg
    13621 ggaaggtagc ggtgcagtac ctcgatttcg gcgttttctt tgtctgccaa atcctggcgg
    13681 ccggcttcag tgtagatttt cgcgctgtct ttgcgctgtt tgaccatttt ggtcaggatg
    13741 gcggtgattt tggcatcgtc ggcttcggtg cgttcgtcca cttcaaactg tttgacggcg
    13801 gcgttgatga ggcggatggt gccgagggaa acttggtctt tggcgcgcat cgcggttttc
    13861 atgtcttcgg taaggcggat tttcaggctc ataatgtcct cgctgggatg tcggatggaa
    13921 acggcgggtt tcgatgccgt ctgaaaagca aaacacaccg cagagcaggt cttgcggtgt
    13981 gtcctcatat cacagggctg acctgtaatc tgtacttgaa cgtttagtac attttgggcg
    14041 gcagttgttg gctgcgcagg cgtttttgca ggcgttttac ggctgccgct tttttgcgtt
    14101 tgcgttcggt agtcggtttt tcgtaggctt cgcgggcgcg cagctcggtc agcaggccgg
    14161 ttttttctac ggcgcgtttg aaacggcgca tagcgacttc aaatggttca ttctctttta
    14221 cgcggattgc aggcatttta tttcctttaa taaattcggt tgtttcatct gcccatcata
    14281 tcggtggaaa gggtagggca gacggtgttg aaggtttacc gtatcgctgt gccttgcggc
    14341 ggcgggatac gcgccgtgac ggaaacatca cctcctaacg ggtcggcttg tgccgtgtca
    14401 agctgcttga aaaagcagga aaaacaattt tcggattgtc ttatatttat ggttcgccgt
    14461 caatgccgtt cgggataaaa atgccgtctg aaagaccggg cgggtttcag acggcatcgg
    14521 tacgtcagcg tgcaggaaca atgcccatac gctgtttgag cgggatgtgc ggcgcgccga
    14581 agagggcggc gtagtaggcg gcattggtca tcactttttt gacatagtcg cgcgtttcgg
    14641 aaaacgggat ggtttcggca tataccgcgc cttcgagggg cgtgtccgcc tgccatcggc
    14701 gcgccctgcc gggaccggcg ttatagcctg cggtggcgag gacttcgttg ttttgcaggc
    14761 ggcgtttggt gtccgccata taccacgtcc ccatacggat attgccgtcg gcggtgtaaa
    14821 gttgtgcggc atccataccg attttgccgg cgatttcgcg cgcggtggca ggcataacct
    14881 gcatcagccc ctgcgcgcct acgcgggatt gcgcgcctat aacgaagcgg ctttcctgac
    14941 gaatcaggcc gtaaacccaa gccggatcga cattaacatt ttgcgcgtgg cggattaccg
    15001 tgtctttaaa cggcgaaata tagcgcaagg tgtagttgag tttgcggtcg gtgcgttccg
    15061 cgctgttgac cgccatatcg taaaaaccgt ggtcgaacgc ggtttgtgcg gcggtcagca
    15121 gcttgtcttc gtcgaacccg cgtgtggcaa aacgccattc cgcctgagcc tgacggcgca
    15181 ttttcgcatc gccggcggtc cggctgtttt ggaacagtac cagcgcacgc ttaatcgcgc
    15241 cgtcttccgc catacggagg acgctgtttg cgtcggcatc gggaacattg ttgcgcgtat
    15301 cgattttgcg ccccaactct tcccctgcca gcaccgcata aaaattcctg cccgtcgctg
    15361 ccgcctgttt gtaaagtttt tccgcctctt gcgtgttgcc cgttgcggcg cggctgcgtg
    15421 ccagccagta gagccaggtc gggctttttt gcagtttttc gggcatatgc gagataacgg
    15481 aggccagctc gtcccaacgt cgggcgcgca aggcggcgcg ggcgtaccac tcgatttggt
    15541 cgtcggtcag ttggcggcgg tcggcaacct tgccgtaata gtccaaggcg gcaggcacat
    15601 tgaggttttg cgactgataa tgccccaata cgccccacgc gaaactgcgt tgttcgaggc
    15661 ttaaaccgct ttccatttcg gacagcaggg cggcggcatt cggcgatttg cgtgcttctt
    15721 tgccgatgac gttcaacaga gcatattcgc gcgaaccttg tgtaccgccg tcaaacgggc
    15781 tgcccaatgc ggcggcaagg ttgcgtgcgt ctgtggtttg gcggccggcc agcagtccgc
    15841 gcacgcgcct ccaggcgtcg ttgccgtcca acaagccgga tgcggctgcc tgttccaaca
    15901 gtttggtgca gcccgaaggc agtttgcccg tatttttgac cagttcggcg gcacgcgtat
    15961 agtcgttgcg gctcgaatcg gcgtagcatt cgacttcttg ggcgcgccct gccggttcga
    16021 gtttggcata ttcctgtgca aacagcgtcc actgtttgcg tgcgcccaaa gacttcagcc
    16081 actcgttgcg gacattttcc gccatcgcac tgtcgccggc gttttccaaa taggcggcga
    16141 cggcggcatc gtttttctgt ttcactgcat ccagtgcgga cgggtagccg ccgtaatctg
    16201 ccagcgtttt tccttccggt tcggcagggc gggtgggaac gcttgccgaa aggtcggcag
    16261 tttctatatt gtctgccggg gtcttgccgg ctggcagtgt gtttgtcgaa gaacacgcgg
    16321 caagcaccag agccgccagc agcggcaggg aatgcttcat agagggtagg tacatcggat
    16381 ttccttaaga atcggaaccc tgaacggtca gggttggaaa agacaaaatg ccgtctgaac
    16441 aggcgtttgc atgaattata tgccgaaact gcaccggctt tggaatgttt ccgacataat
    16501 ttatattttt caatcatttg ccgttttggt gcgaacctct gcctttgccc gtttcagacg
    16561 gcattgtccg aaatggttgc ctgcttcctg ccttattgac aaaaaaacgc tttcccgata
    16621 atatcctacg aaaattaacc tgccgatttg acacagcttg cgggcataac agctaaagcg
    16681 ttccgacaat ttcagcttta tcttccgcgc ccgttgtgtc cgacatcggg ctttgttgta
    16741 tgggaaagac aatgattatt ttggacaagg tttccaagca ttaccaaacg cgcgacaaga
    16801 cccgttttgc cgccgtcgag ccgaccagcc tcgaaatccg cgacggcgaa atcttcgggc
    16861 tgatgggtta ttcgggtgca ggcaaatcca ccctgttgcg cctgattaac ctgttggaac
    16921 gccccgacag cggcaaggtc aacgtctgcg gacaagagct gaccgcgctc gatgccgccg
    16981 cattgcgtca ggctcggcag aatatcggca tggtgtttca gcagttcaac ctgttgaaca
    17041 accgcaccgt cgccggcaac gtcgccttcc cattggaaat cgccggatgg ccgtctgaaa
    17101 aaatcaaagc gcgcgttgcc gaatgcctcg aaatcgtcgg attgaccgaa cgcgccggcc
    17161 actatcccgc ccagctttcc ggcggacaaa aacagcgcgt cggcatcgcc cgcgcgctcg
    17221 catccaagcc gcaagtcatc ctcgccgacg aacccacttc cgccctcgac cccgccacca
    17281 cgcgcagcgt cttggaatgt ttggaagaca tcaacaaacg cttcaacgta accatcgtca
    17341 tcgtaaccca cgaaatgagc gtcatcagac gactgtgcga ccgcgccgcc ctgttggata
    17401 aaggcaaagt cgtcgaaatc gtcgaagtac gcggcaacca aatccacgcc cgatccgaca
    17461 tcgggcgcga actcatcaga gaggactgaa atggcagacc tgaccttcca acaagccgtt
    17521 tccaacatca tcggtatgaa aggcgaaatc gtccgcgcat tgggcgaaac cttcgtaatg
    17581 gtcggcttgt ccaccacatt cgccgtcgtt ttcggcacgt tgctgggcgt gctgctcttc
    17641 gtaacctcca gccgccagtt gcattacaac aagccggtaa atttcctgct cgacaacctc
    17701 gtcaacctca tgcgcgcctt ccccttcgtc attttgatga ttgcgatgat acccgccaca
    17761 cgcgccatcg tcggcagcac catcggcccg attgccgcct cgctggtgtt gagcgtgtcg
    17821 ggattgtttt attttgcccg actggtggaa caaaacctgc gcgaagtccc caaaggcgtg
    17881 attgaagccg ccgccgcgat gggtgcgcct ccgattgcca tcgtctgcaa agtcctcttg
    17941 aacgaagcgc gcgcgggcat ggtttccagc attaccgtgc ttgccatcgg gcttttgtca
    18001 tacagcgcgg cggcagggat gataggcggc ggcggcttgg gcgacctcgc catccgctac
    18061 ggctactacc gctaccaaac cgaagtcatc gtcttcatcg tcgccctcct cgtgctgctg
    18121 gtcatcctga ttcaaagcac cggcaacgcg ttggcgcgga aactcgacaa acgttaaacc
    18181 caaaatgccg tctgaagccc ggatgaagcc ggccttcagg cttgagaacg atacggtagg
    18241 gtgaaatatc cgccgcaccg ttaaaaatgc tataaaatcc ccctgttcgc ggcaaatgcc
    18301 gtctgaacgc cgaatccgga cggcagggtt tcctgcccgt catttttgtt tgaaaccgcc
    18361 acaacatcag gagaaaatat gaaaaccttc ttcaaaaccc tttccgccgc cgcactcgcg
    18421 ctcatcctcg ccgcctgcgg cggtcaaaaa gacagcgcgc ccgccgcatc cgcttctgcc
    18481 gccgccgaca acggcgcggc gaaaaaagaa atcgtcttcg gcacgaccgt cggcgacttc
    18541 ggcgatatgg tcaaagaaca aatccaaccc gagctggaga aaaaaggcta caccgtcaaa
    18601 ctggtcgagt ttaccgacta tgtgcgcccg aatctggcat tggctgaggg cgagttggac
    18661 atcaacgtct tccaacacaa accctatctt gacgacttca aaaaagaaca caatctggac
    18721 atcaccgaag tcttccaagt gccgaccgcg cctttgggac tgtacccggg caagctgaaa
    18781 tcgctggaag aagtcaaaga cggcagcacc gtatccgcgc ccaacgaccc gtccaacttc
    18841 gcccgcgtct tggtgatgct cgacgaactg ggttggatca aactcaaaga cggcatcaat
    18901 ccgctgaccg catccaaagc ggacatcgcc gagaacctga aaaacatcaa aatcgtcgag
    18961 cttgaagccg cgcaactgcc gcgcagccgt gccgacgtgg attttgccgt cgtcaacggc
    19021 aactacgcca taagcagcgg catgaagctg accgaagccc tgttccaaga accgagcttt
    19081 gcctatgtca actggtctgc cgtcaaaacc gccgacaaag acagccaatg gcttaaagac
    19141 gtaaccgagg cctataactc cgacgcgttc aaagcctacg cgcacaaacg cttcgagggc
    19201 tacaaatccc ctgccgcatg gaatgaaggc gcagccaaat aaggcagtcg tataaaatga
    19261 tgccgtctga accgtatccg tgttcagacg gcatttttgt cctttaatcc gccattccct
    19321 gccattccgc cgaatccggc gtatcgattc cgaacagcga caaagcgtgt gcaacactgt
    19381 gcgccactat gtcgtccgcc gtctgcggtt tgcggtacat cgcaggaaca gggggaaaca
    19441 ccacgccgcc catttccgtt acccgcttca tattgtccaa atgggcaagg ttcagcggcg
    19501 tttcgcgcac catcagcacc agccgccgcc tttccttcaa aaccacatcc gccgcacgcg
    19561 tcaagaggtt gtcgccgaag ccgtgcacga ccgaggcaag cgtccgcatc gaacaggggg
    19621 cgaccagcat cccgtccgtt ttaaacgtac cgctggcaat gcacgccccg atattgccga
    19681 tcggatgcac gaagtccgcc aaggcatata cctcgtctct cgcataagcc gtttccgaag
    19741 cgcgcgccat ctccgcacct ttcgatacca caaggtgcgt ttcgatatct tgcgcgcgta
    19801 aaagttccaa agccttcacg ccgtattgga aaccgctcgc cccactgatg ccgattatca
    19861 aacgccgtac catcatccgc ctttcccata aaaccgcctg caacggcaaa ccggctatta
    19921 tagtgaaaaa acagaaatcc gataaatacg gatacaaatt gtcggcaaca cccaatatcc
    19981 gataaaatat ccgatttaac atcctatctg aataggcacg ggagggcggt atggcaaaag
    20041 taaaaggcgg attggggcgc ggtttggatt cgctgctcgc caacggcgca gacaacagca
    20101 gcggcgaccg attgaccacg gttgcggtta aagatatccg gcccggccgc tatcaggcgc
    20161 gtgttcaaat cgatgacgaa gccttgcagg agctggcaga ttcgattaag gcgcaaggcg
    20221 tgatacagcc cgtcatcgtg cgcgaacacg gactgtcccg atacgaactg attgcggggg
    20281 agcggcgttg gcgcgccgca cagattgccg gtctgaccga aatccctgcc gttatcaaaa
    20341 ccatcagcga cgaaaccgca ttggcaatgg gtttgatcga aaacctccag cgcgaaaacc
    20401 tcaaccccat cgaagaagca caaggcttga aacgccttgc cgacgagttc ggactgaccc
    20461 acgaaaccat cgcccaagcc gtcggtaaaa gccgaagcgc gatttccaac agcctgcgcc
    20521 ttttaagcct gcccgaaccc gtgcaggaaa tgctttacca acgccgcctc gaaatggggc
    20581 acgcccgcgc actgctgacc ctgcccgtcg tcgaacagct cgaattggcg caaaaggccg
    20641 tcaaaaacgg ctggtcggtg cgcgaagtcg aacgccgcag ccaagccgcc cttcaaaaca
    20701 aacgtcccga gcccaaaaag actgccgccg ccgacatcgg ccgcctgaat gatttgctga
    20761 ctgaaaaact gggtgtcaac gccgaaatca aaaccgccaa ccataaaaaa ggcaaaatca
    20821 tcctgcattt cgacacgccc gaaaccttcg accatatctt gaagcagttg ggcatagatt
    20881 accgacctta attttgcggg atataccgtc tgaaatatag agaatagctt tccagatttt
    20941 aagtgggaaa tataattcta ttgacatttt tctgcttcac gtaagaatcg ttttcctgtt
    21001 ttcattttta attttcgagg aaattatgaa cacacgcatc atcgtttcgg ctgcgttcgt
    21061 tgcgttggca ttagcaggtt gcggctcaat caataatgta accgtttccg accagaaact
    21121 tcaggaacgt gccgcgtttg ccttgggcgt cagcccaaat gccgtaaaaa tcagcaaccg
    21181 cagcaatgaa ggcatacgca tcaactttac cgcaactgtg ggtaagcgcg tgagccaatg
    21241 ctatgttacc agtgtaatca gcacaatcgg cgttaccact tccgatgcaa tttgtttggg
    21301 aggcggaacg cacaaaggca aaagtcaatg caatgctttg cttaaagcgg caggccgttg
    21361 ctaatccttt attcg
//
LOCUS       DAWFIE010000030        20716 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWFIE010000030 DAWFIE010000000
VERSION     DAWFIE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20716
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.030"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(838..1422)
                     /gene="rnhB"
                     /locus_tag="WGQ71_001035"
     CDS             complement(838..1422)
                     /gene="rnhB"
                     /locus_tag="WGQ71_001035"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ0707058.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKEQAAAWYVAAATPEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDG
                     NRIPEHLDIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQTLF"
     gene            complement(1490..3385)
                     /gene="mnmG"
                     /locus_tag="WGQ71_001036"
     CDS             complement(1490..3385)
                     /gene="mnmG"
                     /locus_tag="WGQ71_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ0707059.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIQGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEEQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3710..5137)
                     /locus_tag="WGQ71_001037"
     CDS             complement(3710..5137)
                     /locus_tag="WGQ71_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0707060.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            5297..6286
                     /gene="pdxA"
                     /locus_tag="WGQ71_001038"
     CDS             5297..6286
                     /gene="pdxA"
                     /locus_tag="WGQ71_001038"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ0707061.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAAEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFCIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            complement(7274..10033)
                     /locus_tag="WGQ71_001039"
     CDS             complement(7274..10033)
                     /locus_tag="WGQ71_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ0707062.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYKGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTARQEARADTAET
                     RNNGNRRRNERGDRAAERANEAEIQSRNVQPAATVADAAPSETEVQTGKRRRNGSRNE
                     RGQTAPETAAVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSENATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEAATVPAEEMIQV
                     ETRQG"
     gene            10527..11519
                     /locus_tag="WGQ71_001040"
     CDS             10527..11519
                     /locus_tag="WGQ71_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0707063.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPLDLAQFAVMLENGTKM"
     gene            11567..12739
                     /gene="lpxB"
                     /locus_tag="WGQ71_001041"
     CDS             11567..12739
                     /gene="lpxB"
                     /locus_tag="WGQ71_001041"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ0707064.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     TGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRAGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHSMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLKRYPAARFLLPAATEATKRRLAEILQRSEFAGLPLTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQHDAVPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            12747..12992
                     /locus_tag="WGQ71_001042"
     CDS             12747..12992
                     /locus_tag="WGQ71_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707065.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNEAELARVGLRL
                     KYNGLLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            12994..13404
                     /locus_tag="WGQ71_001043"
     CDS             12994..13404
                     /locus_tag="WGQ71_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707066.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPMPNYSEWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEILDYGGA
                     LEVIGGRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            13422..13667
                     /locus_tag="WGQ71_001044"
     CDS             13422..13667
                     /locus_tag="WGQ71_001044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707067.1"
                     /translation="MPRKQLYRISFSVETPPLGHLSFGAVESDFIWEGRNPFQIRTTH
                     RATLYVSSRVLKHMPIRSLSAKCRLNNIFSDGILYGG"
     gene            complement(13664..14473)
                     /gene="dapB"
                     /locus_tag="WGQ71_001045"
     CDS             complement(13664..14473)
                     /gene="dapB"
                     /locus_tag="WGQ71_001045"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ0707068.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(14483..14860)
                     /locus_tag="WGQ71_001046"
     CDS             complement(14483..14860)
                     /locus_tag="WGQ71_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0707069.1"
                     /translation="MNKTLILALSALLGLAACSVERVSLFPSYKLKIIQGNELEPRAV
                     ASLRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKDRSNLTVYFENGVLV
                     RTEGNALQNAAEALRVKQNADKQ"
     gene            15079..15513
                     /gene="fur"
                     /locus_tag="WGQ71_001047"
     CDS             15079..15513
                     /gene="fur"
                     /locus_tag="WGQ71_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ0707070.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            15583..16308
                     /locus_tag="WGQ71_001048"
     CDS             15583..16308
                     /locus_tag="WGQ71_001048"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ0707071.1"
                     /translation="MRIPLLAPDNYAFPDPAYALAWCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVVCKGL"
     gene            complement(16452..17483)
                     /gene="gap"
                     /locus_tag="WGQ71_001049"
     CDS             complement(16452..17483)
                     /gene="gap"
                     /locus_tag="WGQ71_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0707072.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTIEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(17684..18523)
                     /locus_tag="WGQ71_001050"
     CDS             complement(17684..18523)
                     /locus_tag="WGQ71_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ0707073.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRVCPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            18672..20648
                     /locus_tag="WGQ71_001051"
     CDS             18672..20648
                     /locus_tag="WGQ71_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ0707074.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGETGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
BASE COUNT         4081 a         5006 c         6455 g         5174 t
ORIGIN      
        1 tcctgatttt tgttaatcca ctatatcgtc attcccgcgc aggcgggaat ctagatctgt
       61 cggtacggaa acttatcggg aaaaacggtt tttccaaacc tgagactcca gattcctgtt
      121 ttcgcgggaa tccggttttt tgagtttcag tcatttttga taaattcttg cagctttgag
      181 tttctagatt cccgcttttg cgggaatgac gcggaaaagt tgctgtgatt tcggataaat
      241 tttcgtcacg cttaatttct gttttatccg ataaatgcct gcaatctaaa atttcgtcat
      301 tcccgcaaaa acaaaaaatt aaaacagaag cctaaaattt cgtcattccc gcgaaggcgg
      361 gaatctaggt ctgtcggtac ggaaacttat cgggtaaaac ggttttgcca gccctgagac
      421 tccggattcc tgttttcgta ggaatccggt tttttgagct tcagtcattt ttgataaatt
      481 cttgcagctt tgagtttcta gattcccgcc tgcgcgggaa tgacgaaatt tcagtttttg
      541 atttctttcg tttttgcggg aatagcggtt gggaagtttc ccgaaattca aaaaaaaaac
      601 ggaaaccgga cggattggat tcccgttttc acgggaatga cggattttag gtttcttttt
      661 tgattttctg ttttttgcgg gaatggcggt ttggaagttt cccgaaactt aaaaacaact
      721 gaaacccgaa aaattaaatt accgcctgcg cgggaatggc ggtatgggtt ctttctcttt
      781 gaagttacga tgctgaaaat gccgtctgaa ggcttcagac ggcattttta tgccggttta
      841 aaacagcgtc tgctgcgcga gcaggtttct gacgggggcg aaatctcggc ggtgttcggg
      901 cagcacgccg tattgtttga gggcttccag atgctgcttc gtgccgtaac ctttgtgttt
      961 gtcgaaaccg tattggggat ggcgttgcgc cagtgcgtac atttccgcat cgcgggcggt
     1021 ctttgccaaa acggatgcgg cggagatttc gatgattttg ctgtcgcctt tgacgactgc
     1081 ttcggcgggg atgtccaaat gttcgggaat gcggttgccg tcgatgaata ttttttcagg
     1141 acgcacagcc aaaccgtcaa cggcgcgttt catggcgagc atcgtggcgt gcaggatgtt
     1201 gaggcttgcg atttcttcgg gcgtggcggc ggcgacgtac cacgcggccg cctgttcttt
     1261 aatcatttcg gcaagcgcgt cgcgtttttt ctcgctgagt tttttggagt cggtcagtcc
     1321 gggcaggtcg aatgtttccg gaaggatgac ggcggcggca aacacgctgc cgaccaaagg
     1381 cccgcgtcct gcctcgtcca cgccggcggt cagtatgtgc atgatgtttc ctgttgggat
     1441 agtaggaatg ccgtctgaaa gggcttcaga cggcatcgta ccgatgtgtt tatttcgcgt
     1501 ctttaaaccc gcgcttcaaa tgcaccatca gcaatgccac tgccgcaggg gttacgccgg
     1561 aaatgcggct tgcttgtccg acggtttcgg gtttgtgctg gttgagcttt tgctgtactt
     1621 ctgccgataa gcctttgact ttgctgtaat cgatgtcgtc gggcagtttt aaggtttcga
     1681 tgtcgcggcg gctgtcgatt tcttcgtttt ggcggtcgat atagccttgg tatttgactt
     1741 ggatttcgac ttgctcgatg acttcggcgg agaggttttc agacggcatc gcgccttcga
     1801 gcgtcatcag cgcggcgtaa tcgaggttcg ggcggcgcag gaggtcgtgc aggttggctt
     1861 cgcggctgag tttttgtccg aacacgcgga tttgttcgtc ttcggcgagc ttttgcggtg
     1921 tgtaccacgt tgttttcaaa cgttggattt cacgttcgat ggcttcacgt ttttcgttga
     1981 acatacgcca ttgttcttca cccaccaagc cgattttgta gccgtcttcg gtcagacgca
     2041 tgtcggcgtt gtcttccctg agttgcaggc ggtattcggc gcggctggtg aacatgcggt
     2101 aaggctcgtt cacgcctttg gtgatgaggt cgtccaccaa tacgccgaga taggcttgtt
     2161 cgcggcgcag caggagcggg tcttgttcgc ggacgtattg cacggcgttt gcgcctgcga
     2221 gcaagccttg cgcggcggct tcttcgtagc ccgttgtgcc gttgatttgt ccggcgaaaa
     2281 agaggccttg gatggttttg gtttcaaggc tggctttgag gttgcgcggg tcgaagtagt
     2341 cgtattcgat ggcgtagccg gggcgcagga tatgggcgtt ttcgaggcct ttcatgctgc
     2401 gcacgagggc gatttggatg tcgaacggca ggctggtgga gataccgttt ggatagtatt
     2461 catgcgtggt cagaccttcg ggttcgagga aaatctggtg gctgtctttg tcggcgaagc
     2521 ggttgatttt gtcttcgata gacggacagt agcgtgggcc gacaccttcg attttgccgg
     2581 tgaacatggg gctgcggtcg aagcctgagc ggatgatgtc gtgggtttgc gtgttggtat
     2641 gcgtaatcca gcaggacact tggcgcgggt gcatttcggc gttgccgcgc acggacatga
     2701 cgggaacggg tgtgtcgccg ggctgttcgg tcagttggga aaagtcaatc gtgcgtccgt
     2761 cgatgcgcgg cggcgtgccg gttttcagac ggccttgcgg cagcttcaat tcgcgcaaac
     2821 gtccgcccag cgatttggcg gcggggtcgc cggcgcgtcc gccctcgtag ttttccaaac
     2881 cgatgtggat tttgccggac aaaaacgtgc ctgcggtcaa caccacggcg cgtgctttaa
     2941 actccacgcc catcgcggtg attacgcctg aaatgcggtc gccatcgagc gttacgtctt
     3001 cgacggcttg ttggaaaagg tcgaggtttt cttggttttc caacatttcg cggatggcgg
     3061 ctttatatag gatacgatcc gcctgcgcgc gcgtggcccg tactgccgcg cctttgctgg
     3121 cgttcagacg gcggaactgg atgccggatt tgtcggttgc caacgccatc gcgccgccga
     3181 gcgcgtcgag ttcgcgcacc aaatgccctt tgccgatgcc gccgatggaa gggttgcacg
     3241 acatttgtcc gagcgtttca atattgtgtg tgagcaaaag cgtctgcgcg cccatacggg
     3301 cggcggcgag cgcggcttct gtgccggcgt gtccgccgcc gacgacgata acgtcgtagg
     3361 ttttggggta aatcatgtgg gtcatgtgtg tattgcctgt cggtgtttca gacggcattt
     3421 atagtggatt aacaaaaacc agtacagcgt tgcctcgcct tagctcaaag agaacgattc
     3481 tctaaggtgc tgaagtacca agtgaatcgg tttcgtactg cttgtactgt ctgcggcttc
     3541 gtcgccttgt cctgattttt gttgaaccac tatattcaat atgccgtctg aaaaacgaaa
     3601 tggattcaaa agtaaagggt tgggattgta cgcttgttcg ccctgttttt acagtgtgcg
     3661 gaaagggaaa agccgcttcg cggggaagcg gctccggtaa gggcgggatt taccaaacgt
     3721 cggatttgat acggcgtttc aggcccggat gttcggaaag tttgaactcg gggtctttgc
     3781 ccattttcag cttggcggtg taatcgcgca gcagcataaa cgccaagggc gagagcagca
     3841 ggatggcgac aaggttgatc cacgccataa tgcccatcgc catatccgcc atatcccaga
     3901 ccaaaggcac attggcaacc gcgccgaaat agacccacgc caaaaccagc atacggaaaa
     3961 cggcggtaat cagccaatgg cttttgatga attggacgtt ggactcggca taggcatagt
     4021 tgccgataac ggtggaaaag gcaaacataa acaggatgac ggcgaggaag cccgcgcccc
     4081 attgccccac ttggctgaca atcgccgcct gcgtcagcgc cgcaccgctc aaatcgccgt
     4141 aaggctgttg gtaaatcaag atgatgaagg cggtgcaaga acaaacgatg atggtatcga
     4201 caaacacgcc cagcatttga atcatacctt gcgaaacagg gtgtttcact tcggcggcgg
     4261 cggcggcgtt cggcgcggaa cccatacccg cctcgttgga atacaggccg cgtttgatgc
     4321 ccatcatcat cgtttgcgaa atcagaccgc cgagtaagcc gcctgctgcc gcgtcgaatt
     4381 tgaacgcgcc cgaaaaaatc tgaccgaaca cgtccggaat catcggaata ttggtcaaaa
     4441 tgatgaaaag cgcgataaag aggtacaaaa ccgccatcag ggggacgacg atttccgccg
     4501 ctttagatat gcgcctgatg ccgccgaaga taatcggcgc ggttaaaatc accagggcga
     4561 cgccgacata atgaggctcc caaccccacg ccgctttgac ggtatcggca atggtattgg
     4621 tctgaaccgc ttcaaacaca aagccgaaac agaaaatcag gctcagggcg aacaacacgc
     4681 ccagccattt ctgccccagc ccttgagtga tgtagtaggc agggccgccc cggaaatggt
     4741 ggttgtcgta gtcgcggact ttaaagagct gcgccagcga agattcgaca aacgccgaac
     4801 tcataccgat taaggcggtt acccacatcc aaaacaccgc gcccggcccg ccgactttga
     4861 tggcgattgc cacgcccgcg atattgccca cgcccacgcg gctggcaagg ccggttacaa
     4921 atgcctgaaa cggcgtgatg ccgtgagggt cgtccccctg tttgcggccg ccgagcattt
     4981 ctttgatgct gcgcccgaac aggcggaatt ggacaaagcc cgtggttacg gtgaagaaaa
     5041 gccccgtacc caaaagcata taaaccaagt atgaccacat cggatcgttg atggcgccga
     5101 cccagccgtg cagccattcg gtaaagttct cgttcatatc gcttccttaa agttgaaact
     5161 cgcacatatt ggcggtatgc aagcagggtt taaattttgt aaacgcccat tctagcagat
     5221 tgtcaacaaa atcagaaaaa tttacatcgc cgcgcggctg cggcgttaga atcgcatttt
     5281 gtttggagca aacacgatga aacagcctgt ttttgccgtt acttccggcg agcctgccgg
     5341 catcggcccc gatatttgtt tggacttggc gtttgcacgc ctgccctgcc gctgcgcggt
     5401 attgggcgac aaacacctgt tgcgcgcgcg cgccgaagcc ttgggcaaaa gcgtcgtcct
     5461 gcgcgacttc gatccagaat caggcggcgc ggcatacggc gagctggaag tgctgcacat
     5521 ccctgccgcc gaagcggttg aggcgggcaa actcaatccc gccaacgccg cctatgtgct
     5581 gcaacttttg gacaccgcgc tcgcaggcat ttcagacggc attttcgacg gcatcgttac
     5641 cgcgccgctg cacaaaggca tcatcaacga cgcgcgcgca agcacaggtt ttttcagcgg
     5701 acacaccgaa tatctggcgg aaaaaagcgg cacggggcag gtcgtgatga tgcttgccgg
     5761 caaaggcctg cgcgtcgccc tcgtaacgac ccacctgccg ctgaaagacg ttgccgccgc
     5821 catcacgcaa ccgctgattg aaagcgtcgc acgcattttg catcacgact taaaacacaa
     5881 attctgcatc aaaaatccca aaatcctagt cgccggactc aacccccacg ccggtgaggg
     5941 cggacacctc ggacacgaag aaaccgacac cattatcccc gcgctcgaaa acctgcgccg
     6001 cgaagggata aacctcgccg gcccgtatcc ggcggatacc ttgttccaac cgtttatgct
     6061 cgaaggtgcg gatgccgtat tggcgatgta ccacgaccaa gggctgcccg tgttgaaata
     6121 ccacagtttc gggcagggcg tgaacatcac actggggctg ccctttatcc gcacctccgt
     6181 cgatcacggc accgcgctcg atttggcggc aaccggcagg gcggattccg gcagcctgat
     6241 aactgccgtg gagaccgccg tagaaatggc gcgcggcagc ctttaaagat gataaaagac
     6301 ccgtcatttc cgcgcaggcg gcatcgtcca ccctccgccg tcattcccgc gcaggcggga
     6361 atccagcctc gtcggtacgg aaacttatcg gaaaaaacag tttctccaac cctgcgttct
     6421 agattcccgc ttttgcggga atgacgaatt taggtttctg tttttggttt tctgtccttg
     6481 tgggaatgat gaaattttaa gttttaggaa tttatcggaa aaaacagaaa ccgctccgcc
     6541 gtcattcccg cgcaggcggg aatccagacc tttgggcggc ggcaatattt aaagattatc
     6601 tgaaagtttg aggttctaga ttcccgtttt cacgggaatg acgaaaggtt gcgggaatcc
     6661 ataccttcgg gcggcggcaa tattcaaaga ttatctgaaa gtttagaggt tctagattcc
     6721 cgttttcacg ggaatgacgg aatgttgcgg gaatccggct tgttcggttt cggttttttt
     6781 tgaggtttcg ggcaacttct aaaccgtcat tcccgcgcag gcgggaatcc agaccttcgg
     6841 gcgacggcaa tattcaaaga ttatctgaaa gtttgaggtt ctagattccc gttttcacgg
     6901 gaatgacgaa aggttgcggg aatccggctt gttcggtttc ggtttttttt gaggtttcgg
     6961 gcaacttcta aaccgtcatt cccgcgcagg cgggaatcca ggcctttggg cgacggcaat
     7021 attcaaagat tatctgaaag tttagaggtt ctagattccc gttttcacgg gaatgacgga
     7081 atgttgcggg aatcatggga atgacggaat gttgcgggaa tcatgggaat gacggaatgt
     7141 tgcgggaatc atgggaatgg cggaatgttt cggtaatcac gggaatggcg aagtttcaga
     7201 cggcattgca ggtatccgaa cccatgtaaa aaagaggttc tgcggaacag aacctctttt
     7261 tgccgccgtc ggttcagcct tgccgggttt cgacttggat catttcttcg gcagggacgg
     7321 ttgcggcttc agacggcttg ggctgttcgg aacggcgcaa accgcgtccg gcttggactt
     7381 cgggttgtgc cgcccatgcc ttcaatgcgg cagggtcggt ttcgatcagg acgagtccgc
     7441 cggtttgtgc ggtttcccgt gcctgttccg ccgcagccgt aaaggttgcg ttttcagacg
     7501 gcatttcctg tgcttcggct ttcggtgtcg cgccttcggg caggatggcg gcggtggcac
     7561 ggcggatttt ttccgccgca tcataaaccg gtgcgtcgcc gtttgaaacg gcgggagatg
     7621 ctgtcggaag atccctttct gcaaccggat cggcaatgct gacggtaatc ggcgcgtttg
     7681 cgtcggtttc gccgaaaacg tgcgcggcgg cggaacggac tttgtcggcg gtgtcgtgaa
     7741 tattcaggta ctgctcgatt ttggcggcag acggaatatt gcgttttttg ccgttttgac
     7801 ggcggtcgcg ctgattgttg cgctcgcggc gttctttggc atctcggctg tcgcgttcgc
     7861 ggcggttgcg ttcggatttg ggcttgctgc ctttgtcttc tgcggtatgc ggttcggacg
     7921 gcgtgttttc cgctgtctga acggttgttt cggcaacggc ggcggtttcc ggcgcggttt
     7981 ggccgcgttc gttgcggctg ccgttgcggc ggcgttttcc ggtttgcact tcggtttcgg
     8041 acggtgcggc atctgcaacg gttgcggcag gctgtacgtt gcggctttgg atttccgctt
     8101 cgttggcacg ttcggcggca cggtcgccgc gttcgttgcg gcggcggttg ccgttgttgc
     8161 gcgtttcggc tgtgtcggca cgcgcttcct gtctggcagt tttgcctgcc acttcgcgga
     8221 cttctacttt gctgccttcg cgtttgctgc ggcgcgggtt ttggcggcgg ttgttggcgc
     8281 ggctgccgct gcggtttgcc gtgctgcgtt tttcggaggt ttcggcagcg ggtgcggctt
     8341 gggtttcgct gccgccgaaa atgcgtttga gccaagcttt gaagctgtcc caccaggagg
     8401 tttttttctc gggggcggca gtcggggcgg ggctggtgtg gcgcacgcct ttgacggcgg
     8461 gttcgggacg ggcggctttg gctttttcgc cgccgaacgg tttggcggat tcgtcttctt
     8521 ctggctcggc aacgcgcttg tagctcggtt cgccgtcttc ttctacgtcg tcggtgcgga
     8581 tgcggttgat ttcgtagtgc ggattttcga ggtggatgtt cggaatcagg acgacgttga
     8641 catccaaacg ctcttccatc gcaaacagct cggcgcgttt ttcgttcagc aggaaggtgg
     8701 cgacatcgac gggcacttgt gcgcgcactt cgccggtgtt gtctttcatt gcttcttctt
     8761 gaatgatgcg caaaacgtgc agggcggtgg attcgatgcc ccgaatcacg ccggtgccgg
     8821 cacagcgcgg acacgcaaca tggctgcttt cgcccaaagc cggtttcaaa cgttggcggc
     8881 tcaattctaa aagtccgaaa cgggacagtt tgcccatctg cacgcgggcg cggtcttttt
     8941 tgagcgcgtc gcgcaggacg ttttccacat cgcgctggtg tttggggttt tccatatcga
     9001 tgaagtcgat gacgaccaaa ccgcccaagt cgcgcaggcg catttgtcgg gcgacttctt
     9061 cggcggcttc catattggtt ttgaacgcgg tgtcttcgat gtccgcgccg cgtgtggcgc
     9121 gtgcggagtt cacgtcgatg gaaacgaggg cttcggtgtg gtcgatcacg atcgcgccgc
     9181 cggaaggcag gctgacgctg cgcgaaaacg cgctttcgat ttgatgttcg atttggaagc
     9241 gggaaaacag cggcgtgtgg tcttcgtaga gtttcagacg gcctatattg cccggcatga
     9301 cgtagctcat gaactcggca acttggtcgt aaacttcttg attgtccacc aaaatctcgc
     9361 cgatgtcggg gcggaaatag tcgcggatgg ctcggatcag cagcgagctt tccataaaga
     9421 gcaggtaggg gtcgtgatgc gcttttcctg cttcttcaat cgcctgccag agttgtttga
     9481 ggtagttcaa gtcccattcc aactcttccg cgctgcggcc gatgccggcg gtacgggcga
     9541 tgatgctcat gccgttcgga atgtcgagtt ccgccatggc ggctttcaac tcttgacgct
     9601 cttcaccttc gatacggcgg gatacgccgc cgccgcgcgg gttgttcggc atcaatacca
     9661 gatagcgtcc ggcgaggctg atgaaggtgg tcagcgcggc gcctttgttg ccgcgctcgt
     9721 ctttttcgac ttggacgatg acttccatgc cttctttgag cacgtcttgg atgcgcgcgc
     9781 gtccgccttt gtagtcttgg aagtatgagc gggagacttc tttaaacggc aagaagccgt
     9841 ggcggtcggt tccgtaatcc acgaaacacg cttccagcga cggctcgatg cgggtaatga
     9901 tgcctttgta gatattgcct ttgcgctgtt ctttgcccag cgtttcgatg tccaaatcca
     9961 gcaggttttg tccgtcgacg atggcaacgc gcagctcttc ggcctgcgtt gcgttaaata
    10021 acattctttt catgatcacc tcgtgggcag gcggcgttca gacggcacat gcccggttcg
    10081 gcattccgta aggctgggtt ttccgatgtt ttcggataaa accggtaatc agtttttgag
    10141 ttgaaaatcc gcagggatgc acgttccgga gaaccgtgtg cggaagggtc ggatacagaa
    10201 ggctataaag atcgatgcgg cggtctgtct gccgcgttcc gagcggtatg gtcggaaaaa
    10261 tgggtgccgg cttcttcttg ttatcgtgat gcctgtgttt tcgggcggtt tgcgtttggg
    10321 gcttgggccc ggctgccgtc ttacttccgc gccgaaacgg caaaatcaat tcaaacttga
    10381 ctacgttctg cgcctgccgg ctgggaacag gcgcagggaa aatgctttgc ggagtgcgtt
    10441 tttaatataa aattccgttt taaagtaaac cgtttcagga ggcgcggcgg gcgcgctttt
    10501 tgctgaaacg gatgttcgga ttatagatga aaacgcacga aataagcaaa gattcggtca
    10561 gcttgatagg ggttgccgaa catgaggcgg gtcaacgcct tgataactat ctgataaaaa
    10621 tcctcaaggg tgttcccaag agccatatcc accgcattat ccgcgccggc gaggtgcggt
    10681 tgaacaagaa acgctgcaaa cccgacagcc gtattgcgga gggggatacg gtgcggattc
    10741 cgcctgtgcg cgtggcggag aaggaaatgc cgtctgaaag gcgtgccgcc gtaccggcgc
    10801 gtgcgtttga cgttgtttac gaagacgatg cgcttttggt catcgacaaa ccgtccggcg
    10861 ttgccgtcca cggcggcagc ggcgtgagtt tcggcgttat cgaacagttg cgccgcgccc
    10921 gtccggaggc gaagtatttg gagttggttc atcgtttgga caaggatacg agcggcttgt
    10981 tgatggtggc gaagaaacgc agcgcgctcg tcaaacttca cgaagccatc cgtaacgacc
    11041 accccaaaaa aatctacctt gcgctggggg tgggcaaact gccggacgac aatttccatg
    11101 tcaaactgcc cctgttcaaa tataccggcg cacaaggcga aaagatggtg cgcgtcagtg
    11161 cggacgggca gtcggcgcat acggtgttcc gtgtgttaag ccgtttttca gacggcattt
    11221 tgcacggtgt cgggctgtcg cacctgactt tggtgcgggc gacgttgaaa acggggcgca
    11281 cgcaccaaat ccgcgtccac ctgcaatctc aaggctgtcc gattgcgggc gacgaacgct
    11341 acggcgatta tcaggcgaac cgtcgtttgc agaagttggg tttgaagcgg atgtttttgc
    11401 acgcgtccga gctgcacctg aaccatccgc tcacgggcga gccgctggtg ttgaaggcgg
    11461 agctgccgct ggacttggcg cagtttgcgg tgatgttgga aaacgggacg aaaatgtgaa
    11521 ccccgatgcc gtctgaagcc ttcagacggc atcgggacgt gaaagtatgt ggggacagac
    11581 gaatatggtt gataaaaaaa gccctttgat tgccgtcagt gtcggcgaag cgtcgggcga
    11641 cctgttgggg gcgcacctga tacgcgccat ccgcaagcgt tgcccgcagg cgcggtttac
    11701 cggcatcggc ggggaactga tgaaggcgga aggtttcgag agcctttatg atcaggagcg
    11761 gctggcggtg cgcggctttg tcgaagtggt caggcggctg ccggaaattt tacggatacg
    11821 cagggggctg gtacgggatt tgctgtcgtt gaaacctgat gtctttgtcg gtatcgatgc
    11881 gcccgatttc aatttgggtg tggcggaaaa gctgaaacgg gcgggcattc cgaccgtgca
    11941 ttatgtcagc ccgtcggtgt gggcgtggcg gcgggaacgc gtgggcaaaa tcgtgcatca
    12001 ggtcaaccgc gtgctgtgcc tgttcccgat ggagccgcag ctttatctcg atgcgggcgg
    12061 acgtgcggag tttgtcggtc attcgatggc gcagcttatg cccttggaag acgaccgtga
    12121 aacggcgcgg caaactttgg gcgtggatgc cggcatcccc gtattcgccc tgctgcccgg
    12181 cagccgcgtc agcgaaatcg actatatggc gccggtgttt tttcagacgg cattattgtt
    12241 gttgaaacgc tatcccgccg cacgcttcct gctgcctgcc gcaacggagg cgacgaagcg
    12301 gcgtttggcg gaaattttgc agcgttcgga gtttgccgga ttaccgctga cggtaaccga
    12361 cagacagtct gaaacggtgt gcagggcggc ggatgcggtg ctggtaacga gcggtacggc
    12421 gactttggaa gtggcgctgt gcaagcgtcc gatggtcatc agctacaaga tttcgccgct
    12481 gacttatgct tatgtgaaac gcaaaatcaa agtgccgcat gttggcctgc cgaatatcct
    12541 gttgggtaag gaggctgtgc cggagctgct tcaacatgat gccgtaccgg aaaaactggc
    12601 ggcggcgttg gcggactggt acgaacaccc cgataaggtt gccgcgctgc aacaggattt
    12661 cagggcgttg cacctgctgt tgaaaaaaga tacggcggat ttggccgcgc gcgcggtttt
    12721 ggaagaggcg ggatgttgag cggttaatgg attattttcc cgaagcagca cgtattacaa
    12781 aaaaaggggg agaaattgtg attaatggca catcaaacaa taagtattta agaggaattc
    12841 caaatgaagc agaactggct cgagtgggat taaggttaaa atataatggt ctgttaaagg
    12901 aagagtttaa acaactgaaa tttcataaga gtagtagaac caacttggac tcaaaaaatc
    12961 ttaaactcat tgtttttgaa aaggtaaaat aatatgacaa cttataccat tccaaaaaaa
    13021 gattatcaat ttctgtatat atatgagggc actctattaa actatacttt gaaaaacgat
    13081 gaattccata tcatcgtcca gaatgtggat tatccggact ttcctcaaga gatccctatg
    13141 cccaattatt cagaatgggt aaaaattaaa ttcaagcagt tcagctatct gaaatttatc
    13201 tatggatacg ccacgaagaa ccaagataaa aatatcaaaa atgtattgga acttggagaa
    13261 ttaaagcagg atgatgaaat cttggattat ggaggtgcgc tggaagtgat aggcggtagg
    13321 tatgatcttc cgaccggttt tagtatagat atagtttgcc gggaaataga gttagaattt
    13381 ttagatcagg agagttttaa ttaaacgagc cgtaacttgt catgccgcgc aagcaacttt
    13441 atcgtatttc cttttcggtt gaaaccccgc cactcggaca tctgtccttc ggggcggtag
    13501 aatcagactt tatttgggag gggcgtaatc ccttccaaat caggacgaca catagggcga
    13561 cgctttatgt atcgtcccgt gtgttgaaac atatgccgat acggagcctg tcggcaaaat
    13621 gccgtctgaa caatatcttt tcagacggca ttttgtatgg gggttaacgg ttgttcagcc
    13681 cgagtacgtc ctgcatatcg tacaaacccg ttttgccgtt tacccaaact gcggcgcgga
    13741 cggcgccggc ggcaaaggtc atgcggctgc cggctttgtg ggtgatttcc acgcgctcgc
    13801 cgtcggtggc gaagagggcg gtgtggtcgc cgacgatgtc gcctgcgcgg acggtggcaa
    13861 agccgatggt cgacggatcg cgcggaccgg tgtggccttc gcggccgtaa acggcgcatt
    13921 gtttgaggtc tctgccgagc gcgccggcga tgacttcgcc catgcgtaac gcggtgccgc
    13981 tgggggcatc gactttgtgg cggtggtggc cttcaatgat ttcgatgtcg tagccttcgt
    14041 ttaacacgcg ggcgacggtg tcgaggatgt ggaaggtgag gttgacgccg acgctgaagt
    14101 tggcggcgaa aacgatgcct gtttgttcgg cggcagtgtg gatagcggct ttgcccgcat
    14161 cgtcgaagcc tgttgtgccg atgatgatgt tgacttgttt ttcaacgcat ttttgcaggt
    14221 gtttgagggt gggctcgggg cgggtgaagt cgatgagtac gtcgctttgt gcgagaacgg
    14281 cgtcaacgtc gtctgaaatg gcgatgccgg ttttgagtcc gacggcgtag cctgcgtcca
    14341 gcccgagggc ttctgagcct gagtgttcaa gcgcaccgga aaggacggtg tcgggatggt
    14401 tgttgacggc ttcgacgagg acgcgcccca tacggccgtt tgcgccggcg atggcgattt
    14461 tgagcggtgt catgtgtgtt ccttattgtt tgtctgcgtt ttgttttacg cggagggctt
    14521 cggcggcatt ttgcagggcg ttgccttcgg tgcggacgag cacgccgttt tcaaaataga
    14581 cggtcagatt gcttcggtct ttgatgatgc cgttgcggga ggtgttgaag gtatagtccc
    14641 agcggtcggt atggaatgcg tcgcgcagta tggggctgcc gagcaggagc aggacttggt
    14701 ctttggtcat accggggcgg agggaggcga cggcgcgagg ttcgagttcg ttgccctgta
    14761 tgattttgag tttgtacgag gggaacagtg aaacgcgttc gacgctgcac gcggcaaggc
    14821 cgaggagggc ggaaagggcg aggatgaggg ttttgttcac ggaaatgcct ttctgtgcaa
    14881 atcgggatgg gtagtgtaac actgcttgaa tattttataa aagcgaacga taatcatacg
    14941 attaagcggt atccgccctg tccgcgcatc ggccgccggt gcggttttac tattgcaaac
    15001 tgctatggtg cgatagtggg caaacaggcc gaaattgcgt attataacgt ctattgtttt
    15061 acaggggtat tgaatattat ggaaaaattc aacaatattg cacaactgaa agacagcggt
    15121 ctgaaggtta ccggcccgcg tttgaagatt ttggatttgt tcgagacgca tgcggaagag
    15181 catttgagtg cggaagatgt gtaccgcatt ttgttggaag agggtgtgga aatcggtgtg
    15241 gcgacgattt accgcgtgct gacccagttt gagcaggcgg gcattctgca acgccaccat
    15301 tttgaaacgg gcaaggcggt ttatgagttg gacaagggcg accaccacga ccatatcgtc
    15361 tgcgtgaagt gcggcgaggt aacggaattc cacaatcccg aaatcgaagc cctgcaagac
    15421 aaaatcgctg aggaaaacgg ctaccgcatc gtcgatcacg cgctttatat gtacggcgtg
    15481 tgcagcgact gtcaggccaa gggcaaacgt taaatccgga cggtttgttg ttcagacggc
    15541 attcatgatt ttggatgccg tctgtgtttt tggagaactg ccatgcgtat tccgctgctt
    15601 gcccctgaca attatgcctt tcccgatcct gcctatgctt tggcctggtg cgacgggctg
    15661 gtcggcgtga gcggcgattt ggatgcgggg cggctgctcg aggcgtatcg gaacggcgtg
    15721 tttccgtggt tttctcggga cggttggttt ttttggtatg cggtcggccc gcgtgcggtg
    15781 attgttcccg aacgtctgca cgttccgcgc tcgctggcga aaacgctgcg caacggcagc
    15841 tatcgggttg cggtcaacgg ctgttttgcg gaagtggtcg cgcattgtgc ggcagcggcg
    15901 cgcccgaatc aggacggaac ttggattgcg cccgagtttc agacggcata tttgaagctg
    15961 cacgaaatgg gacacgcgca ttctttcgag tgccattatc ccgatgaaag cggtgaaacg
    16021 aggttggcgg gcggctttta cggcgtgcaa atcggcaggg tgttttacgg cgaatcgatg
    16081 ttcgcattac aaccggatgc gtcgaaaatc gcgtttgcct gcgccgtgcc gtttttggcg
    16141 gatttgggtg tggaactgat agactgccag caggacacgg aacatatgcg ccgtttcggt
    16201 tcggagctgc tgccgtttgc ggattttgcc gaacgtctgc ggatgttgaa cgccgtgccg
    16261 ttgaaagagg aaatcgggcg gcgcgaagtg gtgtgcaagg ggctttgatg gcggcttatg
    16321 cttcggtcag gttcaaatat ggcggattaa aattggaaat gacgacagcc gggtaaaatc
    16381 acggtgaaaa atgccgtctg gaacttgaaa acatcgggtt tcagacggca ttttgtttga
    16441 cggtttgttg cttatttgag tgggcgcact tcaagtccga acatacggcg tgcggtgttc
    16501 agcatttggc agctgaagcc ccattcgttg tcataccaag cgaacacttt gaccatgttg
    16561 ccgtcaacaa ctttggtcag cgttgcgtcg aagtggctgg cttcggtggt gtggttgaag
    16621 tccatggaaa ccaagggcag ggtgttgtag cctaaaacgc ctttgagcgc gcctgcttcc
    16681 gaggcggctt tcatcagtgc gttgatttct tcgattgtcg tgtcgcgcgc ggcttggaag
    16741 ctcaaatcta ccaatgatac gttgacggtc ggcacgcgga tggcaagccc gtcgagtctg
    16801 cctttcaatt cgggcagtac cagtccgacg gcttttgccg cgccggtttt ggtcggaatc
    16861 atgttttcca cgccgctgcg ggcgcggcgc aggtctttgt ggcgcacgtc ggtaacggtt
    16921 tggtcgttgg tcagcgcgtg gatggtggtc atcgcgcctt tgacgatgcc gacgctttcg
    16981 ctcaacactt tggcaaccgg cgagaggcag ttggtggtgc aggaagcgtt ggaaacgacg
    17041 gtcatgtcgg cggttaggat gctgtcgttc acgccgtaca cgacggttgc atcgacatcg
    17101 tcgccgcccg gtgcggaaat gaggactttt ttcgcaccgc tttcgaggtg gattttggct
    17161 ttttctttgc tggtgaacgc gccggtgcat tccatgacca aatcgacacc gagttctttc
    17221 cacggcagtt cggcagggtt gcgggtcgag aagaagggga ttttgtcgcc gttgacgatg
    17281 aggttgccgc cgtcgtggga tacgtcggct tcaaagcgtc cgtgcacggt gtcgaatttg
    17341 gtgaggtggg cgttggtttc aaggctgccg ctggcgttga cggcgacgat ttggagttgg
    17401 tcttgaatct gataatcgta gatggcgcgc aaaacctggc ggccgatgcg tccgtagccg
    17461 ttgatggcga ctttaatgcc catggtttgt tcctttgttg aggggttggg tagattttcg
    17521 gggcggatta tagcaaattt gtagtggcgt gtaattaata ttttattgaa aacagcgcgg
    17581 ccggaagggt gggcggtaag atgcggacgg cacgggtgcg gcggacggag agcttgataa
    17641 aatgccgtct gaaacggttt tcagacggca tatcagggaa gggtcaggag gcggtgtttt
    17701 gtgcggcttc ctgtttggct ttgtattgtt tgaggtattc gagggcggcg gctttttcgc
    17761 tgtcgctgcc gtatttcata tcgcgttggg cgcggcgcaa ctcggcgcgt tcgcgggctt
    17821 cggctatctg tttcgcctga tagtctttgc ggttgtcggc ggcggcgagg cggtcttgtt
    17881 gggtttgcgc ttttgccatg gctttggcga tgaggtcggc agggttaaac gccggttttt
    17941 tcggtgtgtc gggcgtttgc ggacgcgcgt tacggacggc ggcttcgcgt tcggcaagca
    18001 tggccttgcg ttcgtcggct tcgcgctgtt tgcgttcgtt gcgtttgagg tagcgcgcgc
    18061 gcgcgtgttc ggcggcggca aaacggctgt cggcggacag gctgaagcgg cgcgcgcggg
    18121 gcaggacggt gtcggcaacg ggctgcatat ggatgcagtc gacggggcag ggggcgacgc
    18181 agagtccgca gccggtgcat tcgtcggcga tgacggtgtg cataagtttg cccgcgccca
    18241 taatggcatc ggcagggcag acgcggatgc aggcggtgca gccgatacag gcggtttcgt
    18301 ctatccgggc gagtgctttg gcttgggttt tggcaggtgc gacaaggggt ttgccgagca
    18361 gggcggaaat gtcccgaatg acggcttctc cgcccggggc gcagaggttg tacgcttcgc
    18421 ctgttgcgac tgcctgtgcg tagggcaggc agccgtcgta gccgcattcg cggcattggg
    18481 tttggggaag caggcggtct atggcggcgg ctgtggcggt catgtcggtg tgcggctcaa
    18541 aatcgaaagg gcgtatttta gcagaattgt atgccgcgcc cgtttcggat ggtgcgcggt
    18601 gttttgttat aatacggcgg cgtatgccgt ttcagacggc atttttctgt attttcctgt
    18661 tcggacggcc tatgaacgaa ttttcgcttg cccctattgt ggttgttttg ctggtgtcgg
    18721 tcattacggt gatcctgtgc cgcaagttca acattccctc catgctgggc tacctgctgg
    18781 tgggcttttt ggcggggccc ggtatgctca gcctgattcc gaaaagccat gcgacggatt
    18841 atttgggcga aatcgggatt gtgttcctga tgttcagcat cggtttggag ttctcgctgc
    18901 ccaagttgag ggcgatgagg cggctggtgt tcgggctggg cggtttgcag gtcggcgtta
    18961 cgatgctgtc ggtaatgggc atactgatgc tgacgggcgt gccgttcaat tgggcgtttg
    19021 ccgtgtcggg cgcgttggcg atgtcgtcca cggcgattgt gagccggatt ttgtcggaaa
    19081 agacggaatt ggggcagccg cacggtcaga tggcgatggg cgtgctgctg atgcaggaca
    19141 tcgccgtcgt gccgctgatg attctgattc ccgcgctggc gggcggaggg gacggaaata
    19201 tttgggcggc cttgggtttg gcgtttgcaa aaatgctgct gacgctgggg ctgctgtttt
    19261 tcgtcggcag caaaattatg tcgcgatggt tcaggatggt ggcaaaacgc aaatcgtccg
    19321 aactctttat gatcaatgtg ctgctggtaa ccttgggtgt ggcttatctg actgagctgg
    19381 aaggtttgtc tatggcgttg ggcgcgtttg tggcggggat gctgctttcg gaaacggaat
    19441 accgtttcca agtcgaagac gacatccgcc cgttccgcga tattttgctc ggctttttct
    19501 ttatcacggt cggcatgaag ctggacattc aggcattgat cggcggctgg cggcaggtat
    19561 tgatgctgtt ggcaatgctg ctggtgttga aggcactggt tgtgtttgcc attgccttca
    19621 aaatgaaaca ttcggtcggc gacagcctca aaacggcttt gtatctcgcg cagggcggcg
    19681 agttcggctt cgtgatgctg gccattgccg ggcagcttga tatggtttcg ccagaatggg
    19741 aacaggcggc gacggcggcg gttctgctgt cgatgattat cgcgcccttc ctcttgggcg
    19801 gcagcgatgc gctggtcggg cgtttggtca agtcaagctg ggacatgaag tcgctcgatc
    19861 tgcacagtat gctggtagaa accatgagca agtccgacca tgtgctgatt gtcggcttcg
    19921 gcaggggcgg gcagacggtc ggacgcgtcc ttgcccaaga ggatattccg tatttcgcgc
    19981 tcgacttgga cattgcgcgg gtgcaggttg ccagaagtgc gggcgaaccg gtgtcgttcg
    20041 gcgatgcgaa acgcagggaa gtattggaag ccgccggtct gggacgggcg aaaatggtgg
    20101 tggttacgct caacaatatg cacgaaacgc aacacgtttt agacaatgtg ctgtccatgt
    20161 atcccaatat gcccgtatat gtgcgcgcca ccaacgacga ttatgtgaaa acgtttaccg
    20221 atataggtgc ggaagaagcc gtgtcggaca ccaaagaaac cggactcgtg ctggcaggct
    20281 atgcgatgtt aggcaacggc gcgtcgtatc ggcacgtcta tcagacgatg gcaaatatcc
    20341 gccacagccg ttatgccgcg ttggagggac tgtttgtcgg tagtgatgat gaggcaggat
    20401 tcggcgaaac cggcgaaacc gtccgccacg cctttccttt ggctgcagaa gcatacgccg
    20461 tcggcaaaac agtcggcacg cttccgatgg cggcttacgg catcaaactc ttgttcgtcc
    20521 gccgccgcac cggccggatt gaaaacccgg atgcctcgtt tacattggaa ggcggtgacg
    20581 tgttggtggt cgcaggcaaa aaagaagaaa ttatctcttt tgaaaactgg agtttgcagg
    20641 gaatataaat gaaatgccga aataaggctt gcgccatttc cggttatttg gtttaataac
    20701 gctttcgcaa atcgca
//
LOCUS       DAWFIE010000031        20711 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWFIE010000031 DAWFIE010000000
VERSION     DAWFIE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.031"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(63..239)
                     /locus_tag="WGQ71_001052"
     CDS             complement(63..239)
                     /locus_tag="WGQ71_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707075.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(242..1600)
                     /locus_tag="WGQ71_001053"
     CDS             complement(242..1600)
                     /locus_tag="WGQ71_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ0707076.1"
                     /translation="MKPLDLNFICQALKLPMPSESKLVSRIVTDSRDSRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMLAKAWRDNVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNALRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1725..3372)
                     /locus_tag="WGQ71_001054"
                     /pseudo
     CDS             complement(1725..3372)
                     /locus_tag="WGQ71_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(3395..4873)
                     /locus_tag="WGQ71_001055"
     CDS             complement(3395..4873)
                     /locus_tag="WGQ71_001055"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ0707077.1"
                     /translation="MFSKLTPLAETDFPPLPCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIRDLKHRAGILAAQVYGN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIIGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAIINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDNVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPQDIIHDILPAVPHPERVEVDRAVAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4898..6643)
                     /locus_tag="WGQ71_001056"
     CDS             complement(4898..6643)
                     /locus_tag="WGQ71_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ0707078.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPIDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAMKPFTIAKALDSGKVDATDTFNT
                     LPYKIGPATVQDTHVYPTLDVRGIMQKSSNVGTSKLSAMFTPKEMYDFYHDLGVGVRM
                     HSGFPGETAGLLRSWRRWQKIEQATMSFGYGLQLSLLQLARAYTVLTHDGELLPVSFE
                     KQAVAPKGKRVIKASTAREVRDLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKLVNGR
                     YVDNKHVATFIGFAPAKNPRVIVAVTIDEPTANGYYGGVVTGPPFKKIMGGSLNILGV
                     SPTKPLPAAAVKTPS"
     gene            complement(6704..6967)
                     /gene="ftsL"
                     /locus_tag="WGQ71_001057"
     CDS             complement(6704..6967)
                     /gene="ftsL"
                     /locus_tag="WGQ71_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ0707079.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(6964..7950)
                     /gene="rsmH"
                     /locus_tag="WGQ71_001058"
     CDS             complement(6964..7950)
                     /gene="rsmH"
                     /locus_tag="WGQ71_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ0707080.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTMRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIAGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(7947..8402)
                     /gene="mraZ"
                     /locus_tag="WGQ71_001059"
     CDS             complement(7947..8402)
                     /gene="mraZ"
                     /locus_tag="WGQ71_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ0707081.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            8681..9898
                     /locus_tag="WGQ71_001060"
     CDS             8681..9898
                     /locus_tag="WGQ71_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0707082.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQSSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTDSAAYICEAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WAGFRFGDICHKL"
     gene            10030..10106
                     /locus_tag="WGQ71_001061"
     tRNA            10030..10106
                     /locus_tag="WGQ71_001061"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10064..10066,aa:Met,seq:cat)
     gene            10114..10189
                     /locus_tag="WGQ71_001062"
     tRNA            10114..10189
                     /locus_tag="WGQ71_001062"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10147..10149,aa:Ala,seq:ggc)
     gene            complement(10472..11293)
                     /locus_tag="WGQ71_001063"
     CDS             complement(10472..11293)
                     /locus_tag="WGQ71_001063"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ0707083.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEHLFNPLSVAVMLVLGGLFILWVEKRQSWAEPKIADVDALRPIDALMIGVAQVFA
                     LIPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFVAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(11326..11970)
                     /locus_tag="WGQ71_001064"
     CDS             complement(11326..11970)
                     /locus_tag="WGQ71_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0707084.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEREDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKVHLEDRAVAGKWALSQKGFDGKKLMRVYDSPEAAAAASKMQK
                     LTEQYGIDNTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQPPAVQK"
     gene            complement(11973..12836)
                     /gene="ftsN"
                     /locus_tag="WGQ71_001065"
     CDS             complement(11973..12836)
                     /gene="ftsN"
                     /locus_tag="WGQ71_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0707085.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAAKEAEQSDAEKAADKQPVADKA
                     DEVEEKADEPEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            complement(12942..14438)
                     /locus_tag="WGQ71_001066"
     CDS             complement(12942..14438)
                     /locus_tag="WGQ71_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ0707086.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPFEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGERSASVLERVIAARDKQYARQGKVNAALSVSELDTSARIQKEAQEAL
                     GSLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14452..14772)
                     /locus_tag="WGQ71_001067"
     CDS             complement(14452..14772)
                     /locus_tag="WGQ71_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ0707087.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAVQNPEQAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15258..16784
                     /gene="putP"
                     /locus_tag="WGQ71_001068"
     CDS             15258..16784
                     /gene="putP"
                     /locus_tag="WGQ71_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ0707088.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            17035..20640
                     /gene="putA"
                     /locus_tag="WGQ71_001069"
     CDS             17035..20640
                     /gene="putA"
                     /locus_tag="WGQ71_001069"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ0707089.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHGDVVGTVSFADATLAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTGLIAGYAVSLMHEAGILTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRAGEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNGKPETVGISHVAALSP
                     LNSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4871 a         5960 c         5037 g         4843 t
ORIGIN      
        1 cgggcctgcg cccgtttgag gttggcttgc cgcccggcaa ggtttcggac ggcttttgca
       61 gattaacgtg cagggacttt ctcaatcacg caaatcgggt aactccccgg atttttgccg
      121 ccgcccaagt ctaaacataa atcttcataa agatacgcct gcgtctgcat tccggcataa
      181 aacgcgccga ccagcgcggc aatcaacaat aaggctttaa aaaaacgtcg acttttcatt
      241 ttcacttatc ctccaatgcc tcgaccactt cttccatctg cataaagcgc gaacctttca
      301 ccaacacggt ggcgcgttcg ggcaaatcgt ggcgcaacac ttgaatcaac gggtctttgg
      361 cggcgaacca caaaccgtcc gcgccaaatt tttccgccgc ttcgacgctg ttgtcgccga
      421 caaaataagc cgcttcgatg ccttggtcgc gagcatacgc gccgacttcg gcgtgcatgg
      481 cggcggcttc gtcctcaccc agttcgccca tatcgcccat cacgaaaata cgcggcgcag
      541 gcatacgcgc caacacgtca atcgcggctt tcatgctgtc gggattcgca ttataagtat
      601 cgtcaatcag ggtcgcgccc ttgatgccgg ctttgacgtt cagacggcct ttgatattgc
      661 tgaagccttt caaaccttcc gccacatcgt tcaaactcaa acctgcagcc aaagacagcg
      721 cggcggcagc ggcggcgttg tggacattgt ggcggccggg aacaggcagc accacggcgg
      781 cgcgttcgtc gccgcacacc aaatcaaatt cgcacgacaa cggtttcagc acgatatttt
      841 ccgcgtggac atcgccgcta tcgatgccga aagtgcgcgt attcaaatta agcgttgccg
      901 ttttgaagac agccatattg gcatcttctt gaggaatcag cgccatgccg tctgaacata
      961 agccttgata aatctcgctt ttcgctttgg caatatcgcc cactccgtcg aaaccgcagc
     1021 cgacatgggc acgcagggcg ttgttaacca aggcggcatc aggtttggcg atttgcgtca
     1081 aaaccgccag ttcgccaaaa tgattcatac ccatttcaat cacggcatag cggtgttttt
     1141 cgtttaattt caacaaagtc aacggcaatc cgatgtggtt gttgaagttg cctgccgtcg
     1201 ccaaaacggc atcatcgccg aaacggcggc gcaataccgc agccagcatt tccttcaccg
     1261 tcgtcttgcc gcccgaaccg gtaatgccga acacaaacgg attcacatta tcgcgccacg
     1321 ccttcgccaa catttgcaac gcggtaagcg tgtcatcgac tttcaacgcg ccatccattg
     1381 caacgcaatc ttcgcgcgaa accacaaccg ccgccgcacc agcagccaat acgtcttcaa
     1441 caaaatcatg cgcgtcaaac cgcccgcccg ccaatgcgaa aaacacatcg cccgcgcggc
     1501 tgtcgcggct gtcggttacg atgcgcgaca cgagtttgct ttcagacggc atcggaagct
     1561 tgagggcttg gcagatgaaa tttagatcca gtggtttcat atttactttc gttaatattc
     1621 gggcggcgga cacatcggta gcggctgatt tttttatcgc ctgttttgct gtggtaaaac
     1681 acagattatt ttcccattct cattcggcat tttttctgta cgtatcattt tttagacgta
     1741 tttttagtcg atttgccttt tcccgcatac cacggcgcgg ggtcgtcggg cagtccgtcg
     1801 ataaaggcaa ggttattgcc ttcgccctgc acatcgggaa cattccccca aaaatcatag
     1861 ccgtcatcgg gcaactcgtc ggtttcgata cccgtccaac tgccgaatcc gcgtaaaaaa
     1921 ttaaacgcgc tgcgcctcac tttaatcatt tcgcgccgcg tgtcatcgga cgaaatttta
     1981 accagcggca cgccgtagct ttgacgcttc cacgcaccat gtgtcaacgt tcgctcgccg
     2041 cctttaccga catgcatcaa accgtggtcg gaaaagtaaa ccaaagacca gctttcttta
     2101 ttttcattca atatcttaac tgtatcttct aaaaatttat cggtttgcgc gatggtggaa
     2161 acatagcagg atattttttc agtttgatac tgaaaccgcc gcgcatcctt atccaaacgt
     2221 gtgcaaaaat cactgtgcga acccatcagg tgcatcacaa tcagccgagg cttcgtgcct
     2281 gcatttccta tcaaaacccg tttgaacgcc ggcaacaaaa ggcggtcgct caaccccgcg
     2341 cttttgccat aatcacccct ttgggtaaac cacggataat cgctgcgtag ggcataggtg
     2401 gaaatttcgt tggcaaaatg ccccaacatt ccttgattag acagccacgc cgtccgaaaa
     2461 cccgcctgct tcgccaagct gacgatgtta ttgttcggtt ctcccggcag ccccaaagtc
     2521 tgcggcagcg aaagattcgt cgcgtgggcg gtcgattggt aaccgtttat caacagccct
     2581 ttggtctgac tcaaaaaagg cgtatcgggc aatgggaaac cgtaaacatt catataatcc
     2641 gaacgcgcgc tctcaccgat aaccacaaca taatttttat acttgggcgc aacatgacgg
     2701 atatgccatg ttgacgcttt ttttgctgct tccaaaatgt gggcgcgctt ggcggcatat
     2761 tgtgccggcg cggaagccaa atcgtaatac aggtcgaaaa tattcaacaa caggccggca
     2821 tcaggttctc gcaaatcttt atcgctggcg attttatcca tcaccgcgca ggacaaaatc
     2881 aaagtcaata atatagtcag ccatattttg ctgcggcgtt tatagttttt tacgtcagca
     2941 aatacccccc cccgatacac aatatctcca aactgtcaag ccaaaaatga aaaataatgc
     3001 ctgcacaaaa taaagcgacc cgggaagatt gccgacaaat tcacgcgcct cggcaggatt
     3061 gctttccaat atcgaaccga ctatctgata agacggcgca ccatacagcc agccgaccgg
     3121 caaatatagg gcggttgtgc cgacgtaaat cagtaaaaca actgaagaaa cgcgcgggaa
     3181 accgcgtgcc aataataata aaaatacaac cgaagccaaa gcacccacgg cagaataacg
     3241 gtagccgtat tcatattcca agtgataacc tgatgcgatg gccgcgccaa aacaaaaggc
     3301 taaaagcgcg caaatgccca atgtattttt taatgtctga ttcatatttt ttattcgggc
     3361 ggattgtttc atacgatgcc gtccgaatat cccattattt acgagttaac aaagcctgtc
     3421 cgacgatttc aagatcggaa aaacggtgct tcacgccttg tacatcctga tagttttcat
     3481 gccctttgcc ggcaatcaga atgatgtcgt ttgcggcagc ctgctcaacc gcataacgga
     3541 tggcaacggc acgatcaact tcgacgcgtt caggatgagg cacggcaggc aggatgtcat
     3601 ggatgatgtc ttgcggattt tccaagcgcg ggttgtcgct ggttacgacg actttatccg
     3661 cgccctgtac ggctgctgcg cccatcagcg ggcgtttgcc gcgatcgcgg ttgccgccgc
     3721 agccgaatac gcaccataag gccgcaccct gcggtttgat ttcctgcaag gtggcgagtg
     3781 ctttttccaa tgcgtcgggc gtgtgggcgt aatcgacgac gaccaagggc ttgccgctgt
     3841 tcatgatgca atccatgcga cctgaagcgg gacggatttt tgccagcaca tccaatacat
     3901 tatcaagcgg atagccgttg gcgcacagca aggcgatgca ggcggcgagg ttttgcgcgt
     3961 tgaaccgtcc gagcaggcgc gtgcggcatt tcccttcgcc ccacggggtt tggaatacgg
     4021 ctgctatgcc gtctgaagag gcggtgaaat cgataatgcg gatgtcggcg tgttcgctga
     4081 agccgtagct ataaacggcc aaatcgggac agtctttttt cagacgacct acgagttccg
     4141 cgccgtattc gtcatctaca ttgatgatag cgtgcttcaa gccatgccag taaaacagtc
     4201 gcgacttgat ggcaccgtag gcttccatcg tgccgtgata gtcgaggtgg tctcgggtga
     4261 ggttggtaaa gattgcgctg cggaatggca cgccgttgac gcgcgactgg tcaagaccgt
     4321 ggctggagac ttccatcgcg gcggctgttg cgccctgttg gcggaaacgg tagagcaggg
     4381 tttggacatc gacgggggcg ggggtggtgt gcgtggtttc ttccaatgcg ccccaaaagc
     4441 cgttgccgac cgtgccgata atggcggttt tttcgcccaa taaatcggca gcctgcgcca
     4501 gccattgtgt gatggaggtt ttgccgttgg ttccggttac gccccagact ttgaggccgt
     4561 ctgaaacgtt gccgtaaacc tgtgccgcca agataccggc acggtgtttc aaatctctga
     4621 tgccttgatt ggggactttc cattcgggat tccacgcgaa tttgccatcg tcgtcccaaa
     4681 aaacaaaagc cgcgccgttg gcaacggcgg cggggatata actgcggccg tcggcatatt
     4741 cgcccggaca ggcaacgaaa atatcgcctt gtttgatttg gcggctgtct gaatgcaaca
     4801 aacgccctgc cgcgtttgca cacggcagag gcggaaagtc ggtttcagcc aaaggggtta
     4861 acttgctgaa cataaaacaa tctcgttgat actcggatta agacggtgtt ttgacggctg
     4921 cggcgggcag gggcttggtc ggggaaacgc ccaagatgtt caggctgcca cccataattt
     4981 ttttgaaggg cggccctgtc actacgccgc cgtaatagcc gttggcggtc ggttcgtcga
     5041 tggtaacggc cacaatcacg cggggatttt tggcgggggc aaaaccgata aaggtagcga
     5101 cgtgtttgtt gtccacatag cggccattga ccagtttgcg cgccgtaccg gttttcgcac
     5161 ctacgtcgaa accatctacc gcacctgctg tacctgtacc gccgggttcg gttacagaaa
     5221 ccatcaaatc acgaacttca cgcgcggtcg aagctttaat gacacgcttg cctttaggcg
     5281 caaccgcttg tttttcaaaa cttaccggca acaattcgcc gtcatgggtc aagacagtat
     5341 aggcacgcgc caattgcaac aggcttaatt gcagaccata accgaaagac atggttgcct
     5401 gttcgatttt ttgccatctg cgccagcttc tcaacaaacc tgcagtctcg ccaggaaagc
     5461 ctgaatgcat gcgcacgccc acacctaaat cgtgatagaa atcgtacatt tctttaggcg
     5521 taaacatggc agaaagttta ctggtaccga cgttggaaga tttttgcata atgccgcgca
     5581 catccaaagt aggataaacg tgggtatctt gtacggtagc cggaccgatt ttgtaaggca
     5641 gggtattgaa tgtatcggtt gcatccactt tgccggaatc caatgctttg gcaatggtaa
     5701 acggcttcat ggcagaaccg ggttcgatca tgtcggtaac ggcacggttg cgccgctgtt
     5761 cgctgtctgc ccggccgggc ctgttgggat cgtaggcggg cgtattggcc aaggcgagga
     5821 tttcccccgt gcgggcatcc aaaaccacca ccgttccggc ttttgcctga tggtattcga
     5881 ccgccttgtt caactcttca taggccaagg tctgaatcct ctgatcgagg gaaaggatga
     5941 tgtctttgcc gtttttcggg gctttattgc gcggggagtc caagctgtcc acaatattgc
     6001 cctgccggtc ccgcaaaacg acttccgcgc cgtcttcgcc atgcaggctg tcttcaagcg
     6061 aaagttccaa accttcctga cctttgccgt caatatcggt aaatccgatg acgtgtgcaa
     6121 acaggttgcc catcgggtaa tggcgtttta attctttttc aaatacaaag ttttccaaac
     6181 ccaaggcttt gacctcttcg gcaaccttgg gatcgagctg ccgcttaatc caaataaacg
     6241 acttgccttt ctgttcgagc ttgttcctca aaacatcaat cggaacatcg acaagctcgg
     6301 acaggcgttc caattgtgcg gcagacggca tttccttcat ctctttaggc acggcaaaca
     6361 gggactccgt cggcgcactc aacgccaaaa ccgcaccgtt ccggtccgaa accgtaccgc
     6421 gtgtagccgg caatgtttga gtccgcacaa tccggttgtc gccctgttct ttcaaaaagt
     6481 tatacgttac cgtctgcaga tacagtccgc gggcaatcag accggcaaac aagaccgcta
     6541 ttgccatcag gacgaagctg atccgtccgt tactggtcat cggctttttg acctgctctt
     6601 ctttgggcag catccggggc ttatattcac tcttaatcaa catttttact tctcgttatt
     6661 attatcctga cgcaggaatc cgattccggc acacaggctg cttctatctt tgatgcgcca
     6721 ccataaaggt attgcccgaa accggcggat ggaggttttg tttttctgcc gccgccctga
     6781 tcgcttcgtg gttcgccaaa cgcgcctgtt gcagcctcat ttgcgcataa tcctgctcca
     6841 aggcgatttc ctgttttttc gccttatcca aagctgtgaa gttgagcctg tactggtttt
     6901 gctgcatcac aacggaaaaa gcggaaacgc acaccgcaag cagcagaagg aaattcaatt
     6961 tgttcattgc cattcagacg tttttctctg tgattgttcc ggtatcggac cggcagtccg
     7021 ctccgccaca cgcaaaaccg cacttctcgc cctcggattg gcggcaattt ccgcctcacc
     7081 cggctttaat gccctgcccg cgattttcag gggcggctcg ggcaaatccg cttccctgac
     7141 cgccgcccag cgcggcaggg gcgcgtgttg cgaatatttt ttgacaaact gcttcacaat
     7201 gcggtcttcc aacgaatgga aagcaatgac cgccaaacgt ccgccctctt tcagacgaca
     7261 catgacctgc ggcaatactg cccctacttc ttcaagctcg cggttaataa agatgcggat
     7321 tgcctggaag gtgcgcgtcg caggatcctg cccccgctcg cgagtacgga cgttttgtgc
     7381 cacgatctgc gccagcttgc gggttgtatc gattggactt tccgcccgtt gcgcaacaat
     7441 ggcgcgcgca atctggcggc taaaccgctc ttcaccataa ttcttgatta cctcgtgcaa
     7501 atcctgttcc gacgcaaccg ctatccactc tgcggcagac ataccgcgca tcgtatccat
     7561 acgcatatcc aaaggggcat cgaaacgaaa gctgaaaccg cggctgccgt catcgatttg
     7621 cggagacgaa atccccaaat caaacagcgc accgtccacc ttgccgatac ccaaaccgtc
     7681 caatgccgtc tgaaacgaag caaaaccgcc atgcacgaca ccgacccgtt tgtccgaacg
     7741 cgccagctct tctgccacag caatcgcctg cgggtctttg tcgaaaacaa tcaaccgccc
     7801 cgcatcgccc aaacgcgaca aaatcagccg ggaatgccct cccctgccga acgtaccgtc
     7861 cacatagaca ccgtcttcgc gcacggcaag cgcatccacc gcctcattca gcaagaccgt
     7921 gatatgccgg taactttctg ctccactcac aattgcaaat ccgtctgact caactggaag
     7981 gcaagttcgt caggatcgtc atccaaagcc tgaaccatct cagcctccca ctgctcgcga
     8041 ccccaaagct ccaaacggtt ggcacgaccg accaaaacga cttcacggtc gaaatccacc
     8101 cttttcctca gtccggcagg aagcagcacc cggccggcgc tgtcccattc caaaatttcc
     8161 gcgttatgca gcaaaagatt ttgaaaccgc cgcaaaacag ggttatccgc cacttttaag
     8221 tttaaaagtt gcgccgcaac cttttcccac tccgcaacag ggtacatcaa cagcttgtgt
     8281 ttcgactcga gcgttaccac tacggcaggc gtatagaggc gcgacagaat gtcacggaat
     8341 ttggcaggaa cagccaaccg ccccttactg tcgatgctta attcgtgtgc gccgccgaac
     8401 atgacatgtc ccaagccgaa atcaaaatca caagggtaaa agagacactt tgccccacaa
     8461 ttccccacca atcgacacta taagaaattt taaacactcg gtcaaatcag ggcatgaaaa
     8521 ctcattaaca tatctgaaat ttttattcct tttaaaaaca ataagataaa aaatgacgac
     8581 aacggcacgg cgggtgcggt acagaataat cgaaccaata aacaactata tatgattaat
     8641 ttaataatat aaacacaata tatagtatta agataaagcc atgacagcac ccgtaccaac
     8701 gtgtaatatg tcgggaaatc caataaattt acacaagcta acacttatca tgcccctccc
     8761 cctcccctct cccgaagcac ggcaatcctc gctcaaactg caaaccctca ttgccgaaaa
     8821 aatcggtaaa cacggcaact ggattccatt ttcacgtttt atggaattgg ttttatacgc
     8881 tccgcaatac ggctactaca ccggcggcag ccataaaatc ggcaataccg gggattttat
     8941 taccgcaccg accctcacct ctctgtttgc acagacactg gcacgccaac ttcaagaact
     9001 tctatctcaa acggcgggca atatctatga attcggcgcg ggaaccggac agcttgccgc
     9061 cgatttgttg ggcagcattt cggacggcat cagccgttac tatattattg aaatatcgcc
     9121 ggagctggca gcacgtcaga aaaacctgat tcaagcccgc gcaccggaag catctcaaaa
     9181 agttgtccac ttgaccgcac ttcccgaagc gtttgacggc atcatcatcg gcaacgaagt
     9241 actcgatgcc atgcctgtcg aaatcgtccg taaaaatgaa ggcggctcat tcgagcatgt
     9301 cggcgtttgc ctagataatg accgttttac ctattcggca cgaccgctgc acgacttaca
     9361 gctatctgcc ttggcttccc tctattttcc tcaaacagat tatccctaca ccagcgaact
     9421 acatccgcaa caatatgcct ttatccgcac ccttgcctca agacttgaac gcgggggtat
     9481 gatattcatc gactacggtt ttgatgcagc gcagtattac caccctcaac gcaatcaagg
     9541 tactctgatc ggacactacc gacatcacgt tatccacaat ccttttgact tcatcgggtt
     9601 ggccgacctg accgcgcatg tcaactttac cgacattgcc caagccggga cggatgccgg
     9661 attagatttg ataggttacc ttccccaatc ccatttctta ttgaacttgg gcattaccga
     9721 gctattggca cagacgggga aaacggattc ggcagcctac atctgtgaag ctgctgccgt
     9781 tcagaaactg attgaccagc atgaaatggg cgaactgttt aaagtcatcg cattcggaaa
     9841 aaatatcggc atcgactggg caggattccg cttcggcgac atctgccaca aactctaacc
     9901 ctcatgccgc ctgaatccgc ttcagacggc ataaactttt taacatttaa aaacagtcaa
     9961 ctaattcaaa attaaaaaat acggcttgtc aaaaaaacag aaaaacatat aatagcgtct
    10021 tcacgaaacg gcgaattagc tcagtcggtt agagcagagg aatcataatc cttgtgtccg
    10081 gggttcgagt ccctgattcg ccaccaaatt ttcgggggta tagctcagtt ggtagagcgc
    10141 ttgcatggca tgcaagaggt cagcggttcg attccgctta cctccaccag ataaaaaagc
    10201 acagaccgta aaaaggtatg tgctttttta ttgcctgatt gccagcaaat aaagaataaa
    10261 ccactgcctt caaaacaggc aatcgacttt aaacctatcg ccccgcctgt cctgatttta
    10321 tagtgaatta aatttaaacc ggtacagcgt tggctcgcct tgccgtacta tctgcggctt
    10381 cgtcgccttg tcctgattta aatttaattc actatatcag cccgccagac aaacccgacc
    10441 cgaataatgt cttcaggtcg ggtttatggt ttcattccca acttatccag cctgacagcc
    10501 acaatataat gatggcaata ccaaaaacaa tgcggtaata ggcaaaagga atataatttt
    10561 tcttggaaac aaacctcagc aacgctttga ccgccaccaa gcctgaaaca aaggcagcaa
    10621 caaagccaat caaaatcaaa ccgacatcat gcagggtgaa aaaccggtaa tgcttcagga
    10681 catcataagc cgttgcggca accatcatcg gcacagccaa gaaaaacgag aattctgtcg
    10741 cagttttccg ttcgattccc caaagcatcc cgcccataat cgtactgccc gaacgggacg
    10801 tacccggaat cagtgcaaac acttgggcaa cgccgatcat caaggcatca atcggacgca
    10861 atgcatcaac atcggcaatt ttaggctctg cccagctttg gcgtttctcc acccacaaaa
    10921 taaacaaacc gcccaaaacc agcatgaccg ccacactcaa aggattgaac agatgttctt
    10981 taatctgttt gccgaacaac agccccatca cggcggcagg tataaaagca atggcaagat
    11041 tgaggacgaa gcggttggct ttccggtctt ttcccacgcc atgcaacaca ttgctgaaac
    11101 gctgccggta ttcaaacact accgccaaaa ctgcaccgag ctggatggca atttcaaaaa
    11161 ccttgtgatt gctgtgaaaa ccaatcagat tgccgaacac aatcaaatgc ccggtgctgg
    11221 aaatcggtaa aaattcggta aaaccttcta ccaagcccat catcagggct ttcaggacaa
    11281 tcagaaaatc cattgcttgc gcttctttcg gatacgggag ttcggctatt tctgtacagc
    11341 agggggctga cgcttgcgtt cttccctgac tttggcaacc aattctttaa tcgtatgaac
    11401 gccgccgtca aagccattat tgaagataac gcggtatttt ccgccgacaa taacggtcgg
    11461 cgtgttgtca ataccgtatt gttctgtcag tttctgcatt tttgatgctg ctgctgcggc
    11521 ctcgggagag tcataaacgc gcatcagttt tttaccgtca aagccttttt gagacaaagc
    11581 ccattttccg gccaccgccc tgtcttccaa atgaaccttc tgttcgtaaa ctgctttaaa
    11641 cacagcaggg tttgcctgat atttcaaacc cgacaaattg acggcggccg ccatcctggc
    11701 cagaccgagc atttcaggct gccagaccac gtgctccgtc ctcaaatagg catcagacgg
    11761 caatgccttg cccaatttca ataacaaagg atcgaaatga tggcaatgta cgcagaaata
    11821 gccgaaaaat tccaaaacct caattttgcc cgactgttct tgaggaatgg gtttatccaa
    11881 cacaagatag tcttcccttt ccgtcagggc atatgcctgc gcggacaaca ctgccgacag
    11941 cagcagcggc aacagatgtt tgagcttcat aattatttgc tttcgataga acggatcagg
    12001 ctggcgactt catgtttttt caactcgtcc tgcatttttt tcaccgcatc ggcagacata
    12061 ttgccgcttt gcacccggta aagcgtttta tgtcccgcct gataaccgac caccttggaa
    12121 gatatgccca agattgccag tttggcacgc tgtccttccg cgctccggcg gtcggcatac
    12181 gcgcccattt gcagataatg cgttgcttcc gccttgtcgg gcgttttcat tttctgcact
    12241 tctttggcag cggcactgcg cgctttttcg atgctgccgc tgttgaggat ttgttccggg
    12301 gtcggtttgg gtgcaacttt ttccttctcc gcctttttct cttcttttga agcttttttc
    12361 tctgtttctt tagatggttt taccgcttgt tttttaaccg tttcggcatc tttcttctgc
    12421 gctttttccc cgacggtttg ttcacgctct tccgtcagtg ctttcttgcg cactgcctgt
    12481 ccgtccgact tttcccgctc cggctcgtcc gccttttcct caacctcgtc ggctttgtcg
    12541 gcaacgggct gcttgtcggc agctttttcc gcatccgact gctctgcctc tttcgcagca
    12601 tccggttcgg acaaggcgtt ttgatcggcc ggttcaggtt ggatgtcttc cttaggctgg
    12661 tttttcggtt tcaggatttc cgtttctgca ggctgcttcg acgaagccgg gattttgaac
    12721 gcattttgac cgctctggtt cagataaaac aaaataccgg caataatgac cgtcgccagt
    12781 atcaaaccga agaaaaaacc ggacagacct tttccggatt gggaaaattt gttcataaac
    12841 ataccttaat gtgtttcaga cggcattaac gccgtttgct tacgggcgga tattctaaca
    12901 atatcgccat atttgggcaa aacctgcttc cattcccgtt cctataaagc acggcggaaa
    12961 cctatggctt tcatgacgtg gcttctgccg acttcttcgt cgcctgccaa atccgccaat
    13021 gtacgcgcca cgcgcataat gcggtggaag ctgcgggcgg aaagggagag tttttccagc
    13081 aggctgccca atgcttcctg cgcttctttt tggatgcggg cggatgtgtc gagttcactg
    13141 acactcaagg cggcattcac tttgccttgc cgtgcgtatt gtttgtctct agcggcgata
    13201 acgcgttcca aaacggacgc gctcctttcc cctgcttcct gctgcatcag ttcggcggcg
    13261 gacaggctcg ggacttcgat ggtcaaatcg atgcggtcga gcagcggccc ggaaatcttg
    13321 ctgcggtaac gcgcgacgct ttcgggcgtg cagcggcagg gtttgacggg atgcccgaga
    13381 taaccgcacg ggcaggggtt catggcggca acaagttgga atttggcagg atagacggct
    13441 tggcgcgccg cgcgggaaat gtggatttcg ccgttttcca acggttcgcg caaaacttcc
    13501 aaaactttgc ggtcgaactc gggcagctcg tccaaaaaca atacgccgtg gtgcgccaat
    13561 gaaatctcgc ccggacgcgg atccgaaccg ccgccgacca tagccgccgc gctggcgctg
    13621 tgatgcggac tgcggaaagg acggttgctg tcgagttgtt gttggtggtt gggcaggagc
    13681 gaacgcaatg cccaaacttc taccaattcg tcttcggtca gcggcggcag gatgccgggc
    13741 agccgttggg agagcataga cttgcccgtt cccggcggac ccatcatcaa gaggctgtgt
    13801 ccgcctgcgg cagcgatttc caaagcaagg cgcgcggtgt gctgaccttt cacatcgcac
    13861 aaatcaggtt gtccgccatg ttcaaacggc atctgaggaa cttggcattc ggtttgcgcc
    13921 aaaggttcga taccgttcaa atgggcggcg acttcgccca aagagcgcgc gccgtaaacg
    13981 gtaatgccgc gcatcacggc ggcttgtcct gcgttttctt caggcaaaac aaatgcacgt
    14041 tttgcctgca taccctgcca cgccatcgcc aacgcgccgc gcacggggcg caacagcccc
    14101 gacagtgcca attcccccgc aaactcgtat tccgccagtt tttcgggcgc aacctgcccc
    14161 gatgcagcaa ggatgccgat tgcaatcggc aaatcgaaac gccccgactc tttgggcagg
    14221 tcggcggggg cgaggttgac ggtgattttt ttggcgggga attcaaaacc gctttgaata
    14281 atggcggcac ggacgcggtc gcgactttct ttgacttccg tatcgggcag cccgacgatg
    14341 ttgaaatgtg gcaggccgtt ggcaaggtgg gcttccactt cgaccaacgg cgcattcata
    14401 ccgctcaagg cgcggctgta aaccaaggca agcgacatat ttcagacggc cttattcgcc
    14461 ggcttcggtt tgctgcctga tttcggcgac ggcttcttcg gcagcggctt cagccgcttc
    14521 caatgctgcc tgttcgggat tttgcacggc ttcgagtttt gccaaacgcg cttccaaagc
    14581 cgccagtttg gtacgggttt taatcaaaac ctgctgctgg atgtcgaatt cttcgcgcgt
    14641 aaccaaatcc atacggttga acgcgccgcc cagcatcgcc ttaatatttt tttccacatc
    14701 tttggcaggg ctgttggcga tggtttcgct gattttcgag cccacttctt caaaaagctg
    14761 cttgccgaac ataatctgta tccttcctga acatatcaaa ttcaaacggc tattgtataa
    14821 ggaaaaatgc cgtctgaaaa cgggcggcag gttatccgcg gtaaatctac ctcacttcct
    14881 tcgcggttgc taaattacac ctcaaaacac ctgccgcctg aaaacggatt tcatattccg
    14941 cgcaacgcct gattacaaga cactacaaaa caatatgctg ttttaaatga tttttccgac
    15001 gcgcatcgtt tcaaactcgg ctttttaagc cattaagtgc tttgcaaaca acagaaattg
    15061 ggttatgctg aaacggatta tttacaattt catatagttt tattacatat cttattgtga
    15121 ttgaagataa tttatccgaa tccccctttc gggtatccgg attttccgtt gtacttttat
    15181 ttagaaaaac atttcaggcg caagttgctt gcaatttcaa agcctgtaat tctgaagttt
    15241 tagacgagga atagcacatg aatcccatgt acattacttt tgcaatctat ctggttgcag
    15301 tccttctcat cgggcttgcc gcctattttt ccacgcgcaa tttcgacgat tatattttgg
    15361 gcgggcgcag cctaggcccg tttgttaccg cgatgtcggc aggcgcgtcc gatatgtccg
    15421 gctggctgct gatgggtctg ccgggcgcga tttatttgag cggtttgaat gaggcttgga
    15481 ttgccatcgg cctcttggtc ggcgcgtatt tcaactggct cttggtggcg ggccgcctgc
    15541 gcgtgcatac cgaatacgcc aacaatgcgc tgacgctgcc ggattatttc ttccaccgct
    15601 tcggcgcggg cggacacttg atgaaagtgg tttccgcact gattatcctg ttcttcttca
    15661 cgatttattg cgcctcgggc attgtggcgg gcgcaaccct gttccaaagc ctgtttgaag
    15721 gtatgactta caatcaggca atgtggctgg gcgcgggcgc gaccatcgcc tataccttct
    15781 tgggcggttt cttggcggtc agctggacgg atacgctgca ggcttctttg atgattttcg
    15841 ccctgatttt aacgccggtg atggtctatc tgggcttggg cggcgcggaa cagatgtctg
    15901 ccgcgattca aagcgtcgcc gcaggcacgg gcaaagaata cggcagcctg tttgccggta
    15961 cgaccgtcat cggcatcatt tccaccgccg catggggctt gggctatttc ggacagccgc
    16021 acattttggc gcgctttatg gcggccgaaa gcgcgaaatc gctggtatcc gcacgccgca
    16081 tcggcatgac ttggatggcg ttgtgccttg cgggcgcggt ggcggtcggt tatttcggca
    16141 ttgcgtattt cggtgcaaat cccgacaaag tttcttctat gagcggcaac cacgaacgca
    16201 tcttcatcgc gctttccacc ctgctgttca acccttggat cgccggcatc attttgagcg
    16261 cgattttggc ggcggtgatg tccaccctgt cctgccagct tttggtttgc tccagcgcga
    16321 ttaccgaaga cttctacaaa ggctttttgc gtaaaaacgc gcaacagtcg gaactggtat
    16381 gggtcggccg cctgatggtc ttggcgattg ccgtgatttc catcctgatt gcttccgatc
    16441 cgaacagcaa agtattggga ctggtgtctt atgcgtgggc aggtttcggt gcggcgttcg
    16501 gcccgattgt gattctgtcc gtattgtgga aacgcatcac tgcctacggc gcgttgtcgg
    16561 gtatggttgc aggtgcctta accgtggtcg tatgggcgga atgggtgaaa aaaccggctc
    16621 aagcggcagg cgaaagcggc ttgttgacga tgtacgaaat cgtcccgggc tttattgttt
    16681 gcttgatcgt tgccgtgttg gtttccctgt tcaacaaaga accttcgcgc gaaacccaag
    16741 aacgctttga aaaagccgac gcagattacc gcgccgcccg ataaacccct ttcagacggc
    16801 acggcatccc ggttcgtgcc gtctgaaccc gtttccgggt caaacacaaa taaaccttgc
    16861 ctgcatacag gccttatttg tgttttaccc gtttcatttt caaagaaacg gaagcaccta
    16921 ccctgcccgc atttgaagcc ttgtatttca tctgcggatt tattacgata ttaccgtatt
    16981 cagaccgcac cgatgccgtc tgaacccccc gaaaaacttt ggagaatcca aaaaatgttt
    17041 cattttgcat ttccggcaca aactgccctg cgccaagcga taaccgatgc ctaccgccgt
    17101 aatgaaatcg aagccgtaca ggatatgttg caacgtgcac agatgagcga cgaagagcgc
    17161 aacgccgcct ccgagcttgc ccgccgtttg gttacccaag tccgcgccgg ccgcaccaaa
    17221 gccggcggcg tggatgcgct gatgcacgag ttttcactct ccagcgaaga aggcatcgcg
    17281 ctgatgtgtc tggcagaagc cctgctgcgt atccccgaca acgccacgcg cgaccgcctg
    17341 attgccgaca agatttcaga cggcaactgg aaaagccatt tgaacaacag cccttccctc
    17401 ttcgtcaacg ccgccgcctg gggcctgctg attaccggca aactgaccgc cacaaacgac
    17461 agacaaatga gttccgcact cagccgcctg atcagcaaag gcggcgcacc gctcatccgc
    17521 caaggcgtaa attacgccat gcggcttctg ggcaaacagt tcgtaaccgg acagaccatt
    17581 gaagaagccc tgcaaaacgg caaagaacgc gaaaaaatgg gctaccgctt ctccttcgat
    17641 atgttgggcg aagccgccta cacccaagcc gatgccgacc gctactaccg cgactatgtc
    17701 gaagccatcc acgccatcgg caaagatgcg gcaggacaag gcgtttacga aggcaacggt
    17761 atttccgtca aactctccgc catccatccg cgctactcgc gcgcccaaca cggacgcgta
    17821 atgggcgaac tgttgccgcg cctgaaagag ctgttccttt tgggtaaaaa atacgatatc
    17881 ggtatcaaca tcgatgccga agaagccaac cgtctggagc tgtctttgga tttgatggag
    17941 gcactcgttt ccgatcccga tttggcaggc tacaaaggta tcggtttcgt tgttcaagcc
    18001 taccaaaaac gttgtccgtt cgttatcgac tacctgatcg accttgcccg ccgcaacaac
    18061 caaaaactga tgatccgcct cgtcaaaggc gcgtattggg acagcgaaat caaatgggcg
    18121 caagtggacg gcttgaacgg ctatccgacc tacacccgca aagtccacac cgacatctcc
    18181 tacctcgcct gcgcgcgcaa actgctttcc gcgcaagacg ccgtgttccc gcaattcgcc
    18241 acgcacaacg cctacacctt gggcgcaatc taccaaatgg gcaaaggcaa agactttgaa
    18301 caccaatgcc tgcacggtat gggcgaaacc ttgtacgacc aagtcgtcgg cccgcaaaac
    18361 ctaggtcgcc gcgtgcgcgt gtacgccccg gtcggtacgc acgaaaccct gctcgcctat
    18421 ttggtgcgcc gcctgttgga aaacggtgcg aactcttctt tcgtcaacca aatcgtcgat
    18481 gaaaacatca gcatcgacac gctcatccgc agcccgttcg acaccatcgc cgaacaaggc
    18541 atccacctgc acaacgccct gccgctgccg cgcgatttgt acggcaaatg ccgtctgaac
    18601 tcgcaaggcg tggacttgag caacgaaaac gtattgcagc agcttcaaga acagatgaac
    18661 aaagccgccg cgcaagactt ccacgccgca tccatcgtca acggcaaagc ccgcgatgtc
    18721 ggcgaagcgc aaccgattaa aaaccctgcc gaccacggcg atgttgtcgg cacagtcagc
    18781 tttgccgatg ccacgcttgc ccaagaagcg gttggcgcag ccgttgccgc gttccccgaa
    18841 tggagtgcga cacctgccgc cgaacgcgcc gcctgcctgc gccgtttcgc cgacctgctc
    18901 gaacagcaca cccccgcgct gatgatgctt gccgtgcgcg aagcgggcaa aacgctgaac
    18961 aacgccattg ccgaagtgcg cgaagccgtc gatttctgcc gctactatgc aaacgaagcc
    19021 gaacacaccc tgcctcaaga cgcaaaagcc gtcggcgcga ttgtcgccat cagcccgtgg
    19081 aacttcccgc tcgccatctt taccggcgaa gtcgtttccg cattagcggc aggcaacacc
    19141 gttatcgcca aacctgccga acaaaccggc ctgattgccg gttacgccgt ttccctgatg
    19201 cacgaagccg gcatcctgac ttccgccctg caactcgtcc tcggcgcagg cgacgtgggt
    19261 gcggcattga ccaacgatgc ccgcatcggc ggcgtaattt tcaccggctc aaccgaagtg
    19321 gcgcgcctga tcaacaaagc ccttgccaaa cgcggcgaca atcccgtcct gattgccgaa
    19381 accggcggac aaaacgccat gattgtcgat tccaccgcac ttgccgaaca agtctgcgcc
    19441 gacgtattga actccgcctt cgacagcgcg ggacaacgct gctccgccct gcgtatcttg
    19501 tgcgtccaag aagacgttgc cgaccgtatg ctcgacatga tcaaaggcgc gatggacgaa
    19561 ctcgtcgtcg gcaaaccgat tcagctcact accgatgtcg gccccgtcat cgatgccgaa
    19621 gcacagcaaa acctgttgaa ccacatcaac aaaatgaaag gcgttgccaa gtcttaccac
    19681 gaagtcaaaa ccgctgccga tgtcgattcc gaaaaatcca cgttcgtccg ccccatcctg
    19741 tttgaattga acaacctcaa cgaactgcaa cgcgaagtct tcggtcccgt cctgcacgtc
    19801 gtccgctacc gcgccgacaa actcgacagc gtcatcgacc aaatcaacag caaaggctac
    19861 gccctgaccc acggcgtaca cagccgcatc gaaggcacag tacgccacat ccgcagccgc
    19921 atcgaagccg gcaacgttta cgtcaaccgc aacatcgtcg gcgcagtcgt cggcgtacag
    19981 cccttcggcg gacacggtct gtccggcaca ggccccaaag caggcggttc gttctacctg
    20041 caaaaactga cccgcgccgg cgaatgggtt gccccgaccc tgagccaaat cggacaggcg
    20101 gacgaagccg cactcaaacg cctcgaagca ctggttcaca aactaccgtt caacgccgaa
    20161 gagaaaaaag ccgcagcggc cgctttggga cacgcccgca tccgcaccct gcgccgtgcc
    20221 gaaaccgtcc ttaccggacc gaccggcgag cgcaacagca tctcatggca cgcgcccaaa
    20281 cgcgtttgga tacacggcgg cagcacggtt caagcctttg ccgcactgac cgaacttgcc
    20341 gcctccggca tacaggcagt ggtcgaaccc gacagcccct tggcttccta cactgccgac
    20401 ttggaaggtc tgctgctggt caacggcaaa cccgaaaccg tcggcatcag ccacgttgcc
    20461 gccctgtcgc ctttgaacag cgcgcgtaaa caggaacttg ccgcccacga cggcgcactc
    20521 atccgcatcc tcccttcgga aaacggactc gacatcctgc aagtgtttga agaaatctct
    20581 tgcagcgtca acaccacagc cgccggcggc aacgccagcc tgatggcggt tgccgactga
    20641 ttttaccgaa atccccgggc gcggcccgtg aaccaatgcc gtctgaaaac ctttcagacg
    20701 gcatttttat a
//
LOCUS       DAWFIE010000032        19490 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWFIE010000032 DAWFIE010000000
VERSION     DAWFIE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19490
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.032"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..537
                     /locus_tag="WGQ71_001070"
     CDS             <1..537
                     /locus_tag="WGQ71_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0707090.1"
                     /translation="FRSKTTRLFYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGG
                     GPDLEKLKQHENRAIKFYGYLPYADMISIAKGCDIAVNAIHSHAMQSVTNKLSDYMAL
                     QKPILNSQTNAEVLGLLNLLPHENYRSGDVDSFVQAAKDILKRKNDPVQSDEIVRRFR
                     RDISYRKIVNLIERLAHE"
     gene            530..2107
                     /locus_tag="WGQ71_001071"
     CDS             530..2107
                     /locus_tag="WGQ71_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEZ0707091.1"
                     /translation="MSKFFKRLFDIAASASGLIFLSPVFLILIYLIRKNLGSPVFFFQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEMRPGITGWAQVNGRNALSWDEKFACDVWYI
                     DHYSFWLDMKILFLTVRKVFIKEGISAEGEATMPYFTGNQTNEKNNILILSAGRRVEL
                     VQDFQTEAARFSDGISVFATDLNPRMSSACHVSDGSFEVPPISTDSYIDSIFNLAVRE
                     NIGLIIPTIDTELQKLADERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRS
                     PEIYDRKKLVFPCFAKPYDGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFS
                     EFTVDMYYDRQCRLKCAIPRKRLEVRSGEISKGVTCKNSLYQTLLEKMSVLQGARGCI
                     TVQFFYNEETGEFYGVEINPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWEN
                     NLIMLRYDAKVLVHEND"
     gene            2094..2780
                     /locus_tag="WGQ71_001072"
     CDS             2094..2780
                     /locus_tag="WGQ71_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0707092.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDGKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSLQ
                     DLVPIIDSNT"
     gene            3016..3132
                     /locus_tag="WGQ71_001073"
                     /pseudo
     CDS             3016..3132
                     /locus_tag="WGQ71_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            3251..4426
                     /locus_tag="WGQ71_001074"
     CDS             3251..4426
                     /locus_tag="WGQ71_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ0707093.1"
                     /translation="MLNTALSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALSNGTLALDVALKAIGIGAGNDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPNTKAIIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKELWSKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHERFGTNWRMMEMQAVIGRIQLKRLPEWTVRRQENAAKLAESLG
                     KFKSIRLIEVADYIEHAQYKFYAFVKPEHLKDGWTRDRIVNELNVRKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLKNAVELGNTSLMFLVHPTLTDDEIAFCKKHIEAVLAEATQ"
     gene            4474..6384
                     /gene="pglD"
                     /locus_tag="WGQ71_001075"
     CDS             4474..6384
                     /gene="pglD"
                     /locus_tag="WGQ71_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ0707094.1"
                     /translation="MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPVNWQSFLLTALLTIGLFVRMGLYRAVTRFVSFHVLTTAFAGSIVSAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGQAGRQLLEA
                     VKQIREYSAVAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIISKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDNRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAVEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPITLTHQDITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTANETMLPWRELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            6440..7732
                     /locus_tag="WGQ71_001076"
     CDS             6440..7732
                     /locus_tag="WGQ71_001076"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ0707095.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHIEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTR
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(7784..8620)
                     /locus_tag="WGQ71_001077"
     CDS             complement(7784..8620)
                     /locus_tag="WGQ71_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ0707096.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLDLRLINGVEISVTWRGRTIHVVGLDFDERDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RY"
     gene            8629..8871
                     /locus_tag="WGQ71_001078"
     CDS             8629..8871
                     /locus_tag="WGQ71_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707097.1"
                     /translation="MCVVNGGQGVSILSGGVAAVISKNRCMANAKEKPLCGHSRAGGN
                     PDFDLLEMFKVNRYFKLPDSCFRGNGDMTVFNFNLL"
     gene            complement(8914..9123)
                     /locus_tag="WGQ71_001079"
     CDS             complement(8914..9123)
                     /locus_tag="WGQ71_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707098.1"
                     /translation="MDTTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKAKYDAQRRKLKEAGWAEDENAV"
     gene            complement(9101..9415)
                     /locus_tag="WGQ71_001080"
     CDS             complement(9101..9415)
                     /locus_tag="WGQ71_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0707099.1"
                     /translation="MCCLFCGNFGYGGGRCYNNGLFLQGNIMEATVYLEDNEYIALCD
                     LLKLAGLAESGGQAKAFIAEGLVLRNGETETRKTAKIRGGEVIEFDGARLEIADGYDP
                     EA"
     gene            9867..13301
                     /gene="dnaE"
                     /locus_tag="WGQ71_001081"
     CDS             9867..13301
                     /gene="dnaE"
                     /locus_tag="WGQ71_001081"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ0707100.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPIGAADVRIGNPDAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNVHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMAERFSDLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLVKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFTLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQDNIKNTTGDIVDVGTIPLDDQTAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLVDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLDR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMALQTARERYARSLSLALAPHHDIGGLVQLLA
                     AHQLPDTPRIPLQLSYTNEKASGRLQVPPKWTVTPSSALFGELEKLLGSRSVRVNW"
     gene            13579..16827
                     /gene="cas9"
                     /locus_tag="WGQ71_001082"
     CDS             13579..16827
                     /gene="cas9"
                     /locus_tag="WGQ71_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEZ0707101.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEDENPICLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVADNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELNLLFEK
                     QKEFGNPHISDGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAER
                     FIWLTKLNNLRILEQGSERPLTDTERTTLIDEPYRKSKLTYAQARKLLELDNTAFFKG
                     LRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTD
                     EDITGRLKDRVQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIDLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAATKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEEGFKERNLNDTRYVNRFLCQFVA
                     DHMLLTGKGKRRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEG
                     VSVLRVPLTQLKLKDLEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAG
                     NRTQQVKAVRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKG
                     ILPDRAVVQGKDEEDWQLIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNI
                     NIRIHDLDHKIGKNGILEGIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     gene            16894..17808
                     /gene="cas1"
                     /locus_tag="WGQ71_001083"
                     /pseudo
     CDS             16894..17808
                     /gene="cas1"
                     /locus_tag="WGQ71_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
     gene            17801..18127
                     /gene="cas2"
                     /locus_tag="WGQ71_001084"
     CDS             17801..18127
                     /gene="cas2"
                     /locus_tag="WGQ71_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEZ0707102.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     repeat_region   18191..18688
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=18323..18358
                     /rpt_unit_seq="attgtagcactgcgaaatgagaaagggagctacaac"
     gene            19128..>19490
                     /locus_tag="WGQ71_001085"
     CDS             19128..>19490
                     /locus_tag="WGQ71_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0707103.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELIIPDYS
                     TLCRYRNWLAQDDTLSELL"
BASE COUNT         5110 a         5517 c         4738 g         4125 t
ORIGIN      
        1 ttccgctcca aaaccacccg ccttttctac ttgggaacgc tcagttacag ctacgacgtg
       61 gaaaccgtgt gcaaaggtgt tcggaaactt ttagacgacg gcgaaaacgt cgaattgcac
      121 atcatgggcg gcggccccga cctagaaaag ctgaaacagc atgaaaaccg cgccatcaag
      181 ttttacggct acctccccta cgccgacatg atttccatcg ccaaaggctg cgacatcgcc
      241 gtcaacgcca tccatagcca cgccatgcaa tcggttacca acaaactttc cgactatatg
      301 gctttgcaaa aaccgatttt aaacagtcag accaatgccg aagtcctcgg cctcttgaac
      361 ctactaccgc acgaaaacta ccgttccggc gacgtggaca gcttcgttca agccgccaaa
      421 gatattttga aacgcaaaaa cgaccccgtt cagtccgacg aaatcgtccg ccgcttcagg
      481 cgcgacattt cctatcggaa aatcgtcaac ctgattgaaa gattagccca tgagtaaatt
      541 cttcaaacgc ctgttcgaca ttgccgcctc cgcctcggga ctgattttcc tctcgccagt
      601 atttttgatt ttgatatacc tcatccgcaa gaatctgggt tcgcccgtct tcttctttca
      661 ggaacgcccc ggaaaggacg gaaaaccttt taaaatggtc aaattccgtt ccatgcgcga
      721 cgcgcttgat tcagacggca ttccgctgcc cgacagcgag cggctcacgc ccttcggcaa
      781 aaaactgcgt gccgctagtt tggacgaact gcctgaatta tggaatgtct taaaaggcga
      841 catgagcctg gtcggcccgc gcccgctgct gatgcaatat ctgccgctgt acgacaactt
      901 ccaaaaccgc cgccacgaaa tgagaccggg cattaccggc tgggcgcagg tcaacgggcg
      961 caacgcgctt tcgtgggacg aaaaattcgc ctgcgatgtt tggtatatcg accattacag
     1021 tttttggctg gatatgaaaa tcctgttttt gacagtcaga aaagtcttta tcaaagaagg
     1081 gatttcggcg gaaggggaag ccactatgcc ttatttcact ggaaatcaaa ctaatgaaaa
     1141 aaataatata ctgattctgt cggcaggtcg ccgtgtcgaa ttggtgcagg acttccaaac
     1201 cgaagccgca cgtttttcag acggcatcag cgtttttgcc actgacctca acccccgcat
     1261 gtcttctgcc tgccatgttt cggacggttc atttgaagtg ccgccgatta gtaccgattc
     1321 atatatcgac agcattttca atctggcggt acgcgaaaat atcggtttaa tcatcccaac
     1381 catagacacc gaactgcaaa agctggcaga cgagcgcgaa cgctttgaag cggcaagtgt
     1441 ccacatcgtc gtttccgatt cggacttcat cgcacaatgc cgcgacaaac ggaaaaccgc
     1501 caccttgttt gcccgctacg gcatacgctc acccgaaatt tacgaccgca agaagttggt
     1561 tttcccttgc ttcgccaagc cttatgacgg cagccgcgcc atcggtgcaa aacggattga
     1621 cacacctgcc gacctgacac cggaagtctt ggcagacacc aaattaatgt tctgtcagtt
     1681 tatcgacatc gaaaataaat tttccgagtt taccgtcgat atgtattacg accgtcaatg
     1741 ccgtctgaaa tgcgccatcc cgcgaaaacg cttggaagtg cgctccggcg aaatcagcaa
     1801 aggcgttacc tgcaaaaaca gcctctacca aacgctgctg gaaaaaatgt cggtattgca
     1861 gggcgcgcgc ggctgtatca cggtgcagtt cttctacaac gaagaaaccg gcgaattcta
     1921 cggcgtggaa atcaaccccc gcttcggcgg tggcttcccg ctgacgtatg ccgcaggcgg
     1981 caattatccg agccggttga tgcgcgaata cctcggcgac gaagaaatcc cgttttcaga
     2041 cgactgggaa aacaacctca ttatgttgcg ctacgatgcc aaggtcttgg ttcatgaaaa
     2101 cgattaatcc tgaaaccacc gtcatcgtct tcgacttgga cgataccctg tattcggaat
     2161 acgaatacaa gctctccggt atccgttcgg tcatcgatac cgttgccgca ctgtatcccg
     2221 attggaattc agacaaccta tggcgcaaca tcgaccccga cggcaaagat tggctggaca
     2281 aattgtgccg ccactgtggt tttaatgaat cggaaaaaca ggttcttttg tggcaatacc
     2341 ggctgcaccg cccgacgctg actccgtatg ctccgccgga tttcctgtcc gaactgaccg
     2401 cgcccttcgc cgcacgcgcg ctgattaccg acggcagaag cctgacccaa cgcctgaagc
     2461 tggaagcctt gggtctgtct tccctgtttg acgacatcct catctctgaa gcctgctctt
     2521 cggaaaagcc cgacggcaaa cgcttccgcc atcttcaaga caaatatgcc gacaaggcag
     2581 gctgcttcat ctatatcggc gacaacattt ccaaagactt tatcgcgccg aacactttgg
     2641 gctggataac cataggattg cttccctccg gacagaacat ccaccgccac cagcctgaaa
     2701 gttttaacac agaatatcat cccgactttt gggttaactc ccttcaagat ttagttccta
     2761 taattgattc aaatacttaa ccattacata atcaaacatg cactcacaat attaaaagga
     2821 ctatttcatg acaaaatatt attcatgggg cgtaaacggc tttccgccct acgttttcga
     2881 caccatttgg aaacgatgga agttgaatgg gcgcgggggc ggttatcgtg tgcgacatcc
     2941 cggacggcat gaccgtcgcg ggcaatccgg caaagccgct gccgcgcaaa aaccccaaga
     3001 ccgggacggc ataagcgatt aaaatatagt ggattaaatt taaatcagga caaggcgacg
     3061 aagccgcaga cagtacaaat agtacggcaa ggcgaggcaa cgccgtactg gtttaaattt
     3121 aatccactat acacccccgt acagaccgat tttgacaaca cctgcggcgc gcgcccgatt
     3181 cttcggaaca cgcccccttc agacggcaga aatgaggaaa tgccgtctga aaaccgacgg
     3241 acaaaccatc atgctgaaca ccgccctttc cccgtggccc tgcttcaccc aagaagaagc
     3301 cgatgccgtt tccaaagtcc tgctgtccaa caaagtcaac tactggacgg gcaacgaatg
     3361 ccgcgaattt gaaaaagaat ttgccgcctt tgccggcacg cggtacgccg tcgccctttc
     3421 caacggcacg ctggcactcg atgtcgcgct caaagcaatc ggcataggcg cgggcaacga
     3481 tgtgattgtt acctcgcgca ctttcttggc ttccgcttcc tgcattgtga acgcgggcgc
     3541 aaatcccgtg tttgctgatg tggatttgaa cagccaaaac atcagcgcgg aaaccgtcaa
     3601 agccgcgctg accccgaata ccaaagcgat tatcgtggtt caccttgccg gtatgcctgc
     3661 cgaaatggac ggcattatgg ctttggcaaa agaacatgat ttgtgggtga ttgaagactg
     3721 cgcccaagcg cacggcgcaa aatacaaagg caaatccgtc ggctctatcg gccacgttgg
     3781 agcttggtcg ttctgccaag acaaaatcat gaccaccggc ggcgaaggcg gcatggtcac
     3841 caccaacgac aaagagctgt ggagcaaaat gtggtcgtac aaagaccacg gcaagagcta
     3901 tgatgccgtg taccaccgcg aacacgcccc cggcttccgc tggctgcacg aaagattcgg
     3961 cacaaactgg cgcatgatgg aaatgcaggc tgtcatcggc cgcatccagc tcaaacgtct
     4021 gcccgaatgg acggtgcgcc gccaagaaaa cgccgccaag ctggcggaaa gcttgggcaa
     4081 attcaaaagc atccgcctga ttgaagtcgc cgactacatc gaacacgcgc aatataagtt
     4141 ctacgccttc gtcaaacccg aacacctcaa agacggctgg acgcgcgacc gcatcgtgaa
     4201 cgaactgaac gtgcgcaaag tcccctgcta tcaaggcagc tgctccgaag tctatttgga
     4261 aaaagccttc gacaacacgc cttggcgtcc gaaagagcgt ttgaaaaacg ctgtcgagtt
     4321 gggcaacacc agcctgatgt tcttggtaca cccgacgctg accgacgacg aaatcgcgtt
     4381 ctgcaaaaaa cacatcgaag ccgtattggc agaagccaca caataaccct tcagacggca
     4441 tatgccgcct gaaagcacat accgccccac gatatgactc tggaaaccct gatcgccctg
     4501 ccgcgcaaca tcaagaaaat ctgtttcctc atacacgatt ttctgatgat tttcattgca
     4561 ttctggttca cccaaagcct aaaggccgac tactcgaacg aatggttcga ccctgtcaac
     4621 tggcagtctt tcctattgac cgcgctgttg accatcggcc tgttcgtcag aatggggctc
     4681 taccgtgccg ttacacgctt tgtcagtttc cacgtcctga ccaccgcttt tgccggcagc
     4741 attgtttccg ccgtcctgtt cttcctcaac acattgattt ttgaagaaag gctgcgcctc
     4801 gccctgccga ttgtctattt cttactgctg tttgtttccg tgaccggctc gcgtatggtt
     4861 ttgcgcggac tgttgtccga acaccccaaa aaacagatga tccctgtcat catttacggc
     4921 gcagggcagg cgggcagaca actgcttgag gccgtcaaac agatacgcga atattccgcg
     4981 gtcgcctttg tggacgacaa ccccaagctt tggcacaccg tcatctacga ccttgccgtt
     5041 taccagcccg atgccatcgc cttcctcatc gaacgctacg gcgtggaaaa aatcctgctc
     5101 gccatccccg gcgcgaccca ggaacaacgc cgccgaatca tcagcaaact ggaagcctat
     5161 ccctgcgaag tgttgaccat tcccggaatg aaagacctga tggacgggaa aatcagcatc
     5221 ggcacgctca aaaaaatctc cgtgtccgac ctgctcgggc gtgattccgt cgcgcccgac
     5281 aaccgcctca tgagtgccga catcgaaggc aaaaccgtca tggtaaccgg cgcgggcggc
     5341 tccatcggtt cggaactctg ccgccagatt atccgccgcc gccccgaaaa gctgctgctg
     5401 tttgagttat ccgaattcgc cctgtacgcc gtcgaaaaag aattgcgcga aacctgcatc
     5461 caaaaacgcc tcgacaccga aatcctgccc tttctcggtt cggtgcaaaa ccgcaccctg
     5521 ctcgaacacg tcatgaccgc cttttccgtt gcgaccgtct atcacgccgc cgcctacaaa
     5581 cacgtcccca tggtcgaatt caacaccgtc gaaggcatac gcaacaacat cttcggcaca
     5641 ctcgagtgcg cgcttgccgc cacgacatcg ggcgtaagaa ctttcgtcct catctccacc
     5701 gacaaagccg tccgccccac caacaccatg ggtgccagca aacgcatggc ggaactctgc
     5761 cttcaggcgc tcgccgccga acccggacaa aaaacccgct tcagcatggt acgtttcggc
     5821 aatgttttag gttcgtccgg ctccgttgtc ccgctgtttg aaaaacagat tgcagaaggc
     5881 ggcccgatta ccctgaccca ccaagacatt acccgctact tcatgaccat tcccgaagcg
     5941 gcgcaactcg tcatacaggc aggcgcgatg ggtacgggcg gcgacgtatt cgtcctcgat
     6001 atgggcgaat ccgtcaaaat catcgacctt gcccgccaaa tgattacctt aagcggcctc
     6061 aaacccaaaa cacccgaaca acccgacggc gacatcgaaa tcctcattac cggactgcgt
     6121 cccggcgaaa aactctacga agagctgctc atcggcgaca acgtccgcaa aaccggccat
     6181 ccgcgcatca tgaccgccaa cgagaccatg ctgccgtggc gcgagctctc cgccctgctc
     6241 gaccgcatcc gtgcggcctg cgaccgttac gaccagcagg caatccgcac cctgctcatc
     6301 aacgccccga ccggctttgc cccgagcgac ggtatctgcg acctgctttg ggtacgagaa
     6361 acacacagaa aaaatgccgt ctgaaccttc agacggcata acgtacaaac caacctacct
     6421 tacacacacg gagtttgaca tgcagttttc ggcattcggc gaaaaattca cgcaacacag
     6481 cggcatcctc caactgatgg acgacctcgg cgacgcgctc aaaagcgaca agcccgtcaa
     6541 catgctcggc ggcggcaacc cggcgcgcat tccggaaatc gatcgggcgt ttgccgacat
     6601 attctccaaa ctggcggcag aacacgccgt cgaaaacatc ggcaactact ccaaccccca
     6661 aggcgatgcc gcgctgattg acgcgttgac cgccttcctc aaccgcgaat acggctggaa
     6721 cctgaccgcc gacaatatcg cgctgaccaa cggttcgcaa aacgcgtttt tctatttatt
     6781 caacctcttc ggcggcaaat tcaagctttc agacggcaca tccgcagaaa aagccatttt
     6841 gttgccgctc gcgcccgaat acatcggcta tgccgacgta catatcgaag ggcagcactt
     6901 cgtttctgtc aagcccaaaa tcgaaaacgt cgaacacgaa ggcgaagcag gcttcttcaa
     6961 ataccgcgtg gactttgacg cactggaaaa cctgcccgaa ctcaaagcgg gcaaaatcgg
     7021 cgcgatttgc tgttcgcgcc cgaccaaccc gaccggcaat gtgttgaccg acggcgaaat
     7081 ggcacgtttg gacgctttgg cgcgtgaaca cgggattccg ctgattatcg acaacgccta
     7141 cggaatgccg ttccccaaca tcatttacag cgacgtaacg ctgaattggc acgaaaacat
     7201 catcctctgc ttcagccttt ccaaagtcgg cctgccgggc gttcgcaccg gcatcatcgt
     7261 cgccgcgccc gaagtcgtca aagccgtcag cagcctgaac gcgattgtga accttgcccc
     7321 cacgcgcttc ggcgcggcca tcgccgcccc gctgctggaa agcggcgaga tgaaacggct
     7381 tgccgaccaa gtcatccggc cgttttaccg caatcaggcg caaaccgccg tctcgctgct
     7441 caagcgcgag ctgggcgcgt acccgatgaa aatccacaaa cccgaaggcg cgattttcct
     7501 gtggctctgg tttgaaaacc tgcccgtttc ttcgcaaacc ctgtacgaaa tgctcaaagc
     7561 cgaaggtacg ctgattattc cgggcgagca tttcttcgtc ggcatcgaca cgcgggatta
     7621 cccgcacgcg ggcgagtgca tccgcatgag catcgcgcag gacgctcaaa cgctggaaaa
     7681 aggcatcgcc gccatcggca aaaccgtccg aaaactgtac gacaacgttt aaaccgcaaa
     7741 aaatgccgtc tgaaaggttt tcagacggca tttttatctg cattcaatat cgggaaaaat
     7801 gcgcccacac cggcctgcag ttttccggca gttcgggaca cgcgccgaga atgccgccgc
     7861 taaagtcgtt taaacggtgg aagtcgctgc ccgcgctggc gagcataccg aagcgttctg
     7921 ccaaaagcgc gtagttgagg cggtcgtttt tgcagcagtt gccgctgtgg acttcgatgc
     7981 ctgcgccgcc gaggttttta aattcttcaa acagattgcg cttggcggtg gcggacaaat
     8041 cataacgcat cggatgggcg atgaccgcca acccgcccgc gccgttgacg gcggagacgc
     8101 agtcttccag cgtcgcccat tcgtggcgga cggcgcagga tttgccgtcg cccaagtatt
     8161 tggtgaacgc ctgctgcttg tttttgacgt gtcccacccg aatgaggaac tcggcgacgt
     8221 gtgtgcggct gaccatttct ttgtttgccg ccagcgcgag cgcgccgtcg tatgcgccgc
     8281 cgatgccttt cttttcaagc ttggcggcga tggcttcgag gcgtttcaga cggccttgcc
     8341 gcacttgcgc caacaggttt tgcaggtttt cgtcccgttc gtcgaaatcc aaaccgacaa
     8401 cgtgtatggt gcgtccgcgc caagtgacgg agatttccac gccgttaatc aggcgcaaat
     8461 cgagcctgtc ggcttcggca cgcgcttcgg cgatgccgcc ggtgtggtcg tggtcggtca
     8521 acgccagcag cgtgcagccg ttttgatgcg cgaggcggac gacttcggcg ggggagagca
     8581 taccgtcgga aacggtggaa tggcagtgca ggtctatcat aaggcgttat gtgcgtggta
     8641 aatggtgggc agggtgtttc aatattgtcg ggcggtgtgg cggcggtgat ttcaaaaaac
     8701 aggtgtatgg caaatgcaaa ggaaaagccg ctatgcggtc attcccgcgc aggcgggaat
     8761 ccagactttg atttattgga aatgtttaag gttaaccgct atttcaaact tccggattcc
     8821 tgctttcgag ggaatggcga tatgaccgtt ttcaatttta atctactata aaaggtcgtc
     8881 tgaaaacgtg gtttccatcc agccgaaccg ttttcagacg gcattttcgt cttccgccca
     8941 acccgcttct ttcaatttcc gccgttgcgc gtcgtatttg gctttatccg cccagtaaat
     9001 cgtctgaatg cacgcctcgc cgcagtcgct gccgcagcat tcccacgatt cggggcggac
     9061 gggttcgtct aaaagcggct cgcccaaaag ggcttcggct ttatgcttca gggtcgtatc
     9121 catcggcaat ttccaagcgt gcgccgtcaa actcgatgac ttcgccaccg cgtattttgg
     9181 cggttttacg ggtttcggtt tcgccgttgc gcaacaccag cccttcggcg ataaacgctt
     9241 ttgcctgtcc gccgctttcg gcaagtccgg ccagtttcaa gaggtcgcac aaggcgatgt
     9301 attcgttgtc ttcaagatag acggtggctt ccataatgtt cccttgcaga aagagcccgt
     9361 tattgtagca cctgccgccg ccgtacccaa aattaccgca aaacaggcag cacatcccgc
     9421 cccacctgtt ccgtaaaaat gccgtctgaa accccatatg ccgccatccg atcaaagaaa
     9481 tcctgcccaa cggcagactg caaatcctgt tccccgacga atccgcattg acgctgatgc
     9541 acatcctcaa acgcgaactg cccgatacac cggcaatcgg catcaaaacg aaatcaaaaa
     9601 cctgtttgaa cgtaaaatta atttaactgc ttgccatccg tccgaataag gtatatagtt
     9661 ctttataact agtttgatag ttctttatat ctatcaatac tccttgggaa gcctccacca
     9721 cacggtagga ggcatttttt tgccgtcgta aaagctcaaa aacatttgca ggtcatgccg
     9781 tctgaatctg aaacggcatg acctacaccg ccatctaaag acaaccctgc tacaatacgc
     9841 cttttattgt tcacgccgat tttgccatga ccgagccgac ctacatcccc ctgcgcctgc
     9901 ataccgagtt ctcaattacc gacggtatgg tgcggattaa aaaactgatt gccaaagcgc
     9961 aggaatacgg tttgcctgct ttgggcatca gcgatttgat gaacgaattc ggcttggtga
    10021 aattttataa agcctgccgc agcgcgggga ttaagcctat cggcgcggcg gatgtgcgga
    10081 taggcaatcc ggatgcgccc gacaagccgt tccgcgctat gctgattatc cgtaacgatg
    10141 cgggctatct gcgcttgagc gagcttctga cggcggctta tgtcggcaaa gaccgcaatg
    10201 tccatcatgc ggaactcaat cccgaatggc tggaaaacgg cgacaacagc ggcttgattt
    10261 gtttgagcgg cgcgcattac ggcgaagtgg gcgtgaatct cttgaacggc aacgaagacg
    10321 cggcgcgggc ggcggcgttg aaatatgcgg cgtggtttcc cgatgcgttt tatctggagt
    10381 tgcaacgcct gcccgagcgt cccgaatggg aggcttgcgt ttcgggcagc gtgaagctgg
    10441 cggaggaact gggtttgccg gtagtggcga cgcatccgac ccagtttatg agccgcgacg
    10501 atttcaacgc gcacgaggcg cgggtgtgta tcgcaggcgg ctgggtattg acggacaaga
    10561 aacgtccgcg cgatttcaca ccgagccagt ttttcattcc gccggaaacg atggccgaac
    10621 gtttctccga tttgcccgaa gccttggaaa acacggtaga aattgccaaa cgctgcaacc
    10681 tgcacatcac attgggtaaa aacttcctgc cgctcttccc tactccggac ggcctgtcgc
    10741 tggatgatta tttggtgaaa ctgtccaacg agggcttgca ggaacgcatg gttcagcttt
    10801 atcccgacga agcggaacgg gcagcgaaaa tgccggaata tcaggaacgt ttggattttg
    10861 agctgaacat catcatccag atgaaattcc ccggctattt ccttatcgta caagacttta
    10921 tcaactgggc gaaaacacac ggctgtccgg tagggccggg acgcggttcg ggcgcgggtt
    10981 cgctggtggc gtattcgctg aagattaccg accttgatcc gctcaaatac gcgctgctgt
    11041 tcgagcgttt cctaaacccc gaacgcgttt ctatgcccga cttcgacgtg gacttctgtc
    11101 aggcaaaccg cggccgcgtg attgagtacg tgcgcgaaaa atacggcgcg gaggcggtca
    11161 gccaaattgt taccttcggc acgatgtcgt ccaaagcagt catccgcgac gtggggcgcg
    11221 tgttggagct gccgtttacc ctgtgcgaca aactgtccaa gctgattccg ttggaagcca
    11281 acaaacctct gggtttggac gacgcgatga aggcggagcc gcagattcag gaattaatcg
    11341 aagcggaaga agcggacgaa ctgattacgc tggcgaaaaa gctggaagat ttgacgcgcg
    11401 gtttgggtat gcacgcgggc ggcgtattga ttgcgccggg caagatttcc gattacagcc
    11461 ctgtgtatca ggcggacgaa tccgcctcgc ccgtatccat gtacgacaag ggcgacgtgg
    11521 aagacgtggg tttggtgaag ttcgactttt tgggacttcg caacctgacc attatcgaaa
    11581 tggcgcagga caacatcaaa aacaccaccg gcgatatcgt cgatgtcggc acaatcccgc
    11641 tcgacgacca gaccgcctac caaatcttcc gcgatgcgaa caccaccgcc gtgttccagt
    11701 ttgagtcgac cggcatgaaa aaaatgctga aaacggcgca tacgaccaag tttgaagaac
    11761 tcatcgcctt cgtatcgctc taccgccccg gcccgatgga caacatcccc gacttcgtcg
    11821 cgcgcatgaa gggacaggaa ttccaataca tccatccgct gctcgaaggc attctcgcgc
    11881 cgacttacgg cattatggtg tatcaggaac aagtgatgca ggcggctcag attatcggcg
    11941 gctactcgct cggcggcgcg gacctgctgc gtcgcgccat gggtaagaaa aaacccgaag
    12001 aaatggtgaa acaccgcgaa atcttcgccg aaggcgcggc aaaacaaggc atttcgcgcg
    12061 aaaaatccga cgaaatcttc aactacatgg aaaaattcgc cggctacggt ttcaacaaat
    12121 cccacgccgc cgcctacgcc ctgatttcct accagaccgc atggctcaag gcgcactacc
    12181 ccgccgaatt tatggcggcg accatgtcgt ctgaattgga caacaccgac cagctcaagc
    12241 atttctacga cgactgccgc gccaacggca tcgagttcct gccgcccgac atcaacgaat
    12301 ccgactaccg cttcacgccg tatccgaaca tgaaaatccg ctacgcactc ggcgcgatta
    12361 aaggcacggg cgaagccgcc gtcgaatcca tcaccgccgc acggcaaagc ggcggcaaat
    12421 ttaccggcct cttggacttc tgcgagcgcg tcggcaaaga acacatgaac cgccgcaccc
    12481 tcgaagccct gatacgcggc ggcgcgttcg acagcatcga acccaaccgc gccatgctct
    12541 tggcgaacat cgacctcgct atggacaacg ccgaccaaaa agccgccaac gccaatcagg
    12601 gcgggctttt cgatatgatg gaagacgcca tcgaaccggt gcggctcgtc gacgcgccga
    12661 tgtggagcga atcggaaaaa ctcgccgaag aaaaaaccgt catcggcttt tacctctccg
    12721 gccacccgtt cggcccgtat gcccaagaag tccgccaaat cgcaccgacc aaattagacc
    12781 gtctgaagcc gcaagacagc gtgcgcctcg ccggattcgt taccgccgtg cgtacgatga
    12841 tgggcaagcg cggcaaaatc gccttcgtca gccttgaaga tttgagcggg caggttgaaa
    12901 tcatggtcgg cggtcagacg ttggaaaact gcgccgactg cctcaaagcc gaccaagtgc
    12961 tgattatcga atccaaagtc agccgcgacg actacggcgg cggcgacggg ctgcgtattc
    13021 tagcaaacca agtcatggcc ctgcaaacgg cgcgcgaacg ctacgcccgc agcctcagcc
    13081 tcgccctcgc cccgcatcac gacatcggcg gactggtaca actgcttgcc gcccaccaac
    13141 tgcccgacac gccgcgcatc ccgctgcaac tgtcgtatac caacgaaaaa gcgtcgggca
    13201 ggcttcaagt gccgccgaaa tggacggtta caccgagttc cgcattgttc ggcgaactgg
    13261 aaaaattgct cggcagccgg tcggtgcgcg tgaattggta acccaaaata gaaatgccgt
    13321 ctgaagccca aaatcggttt cagacggcat tttttaaccg aaataaacga tgccccttaa
    13381 agcagaagct ttaaggggca gagcgttgcg gcacatcttt tcagacggcc ttattgtagc
    13441 aacggttctc atttcgcagt gcgacaatat gtttgggatt ctagccgttg tgagatgata
    13501 agtcaataat ctatttgtat ataaataaga caatcagata tattatcatt atttgcaacg
    13561 ttgtttacgg agtaataaat ggctgccttc aaacctaatc caatcaacta catcctcggc
    13621 ctcgatatcg gcatcgcatc cgtcggctgg gcgatggtag aaattgacga agatgaaaac
    13681 ccaatctgcc tgattgattt gggcgtgcgc gtatttgagc gtgccgaagt accgaaaaca
    13741 ggcgactccc ttgccatggc aaggcgtttg gcgcgcagtg tccgccgcct gacccgccgt
    13801 cgcgcccatc gcctgcttcg ggcccgccgc ctattgaaac gcgaaggcgt attacaagcc
    13861 gctgattttg acgaaaacgg cttgatcaaa tccttaccga atacaccatg gcaacttcgc
    13921 gcagccgcat tagaccgcaa actgacgcct ttagagtggt cggcagtctt gttgcattta
    13981 atcaaacacc gtggctattt gtcgcaaaga aaaaacgagg gcgaaactgc cgacaaggag
    14041 cttggcgctt tgctcaaagg cgtagccgac aatgcccatg ccttacagac aggcgatttc
    14101 cgcacaccgg ccgaattggc tttaaataaa tttgagaaag aaagcggcca tatccgcaac
    14161 cagcgcggcg attattcgca tacgttcagc cgcaaagatt tgcaggcgga gctgaattta
    14221 ttgtttgaaa aacaaaaaga attcggcaat ccgcatattt cagacggcct taaagaaggt
    14281 attgaaaccc tgctgatgac gcaacgcccc gccctatccg gcgatgctgt tcaaaaaatg
    14341 ttgggacatt gcaccttcga accggcagag ccgaaagccg ctaaaaacac ctacacagcc
    14401 gaacgtttca tctggctgac caagctgaac aacctgcgta ttttagagca aggcagcgag
    14461 cggccattga ccgataccga acgcaccacg cttatagatg agccatacag aaaatccaaa
    14521 ctgacttacg cacaagcccg taagcttttg gagctagaca ataccgcctt tttcaaaggc
    14581 ttgcgctatg gtaaagacaa tgccgaagcc tcaacgttga tggaaatgaa ggcctaccat
    14641 gccatcagcc gtgcgctgga aaaagaagga ttgaaagaca aaaaatcccc attaaacctt
    14701 tctcccgaat tacaagacga aatcggcacg gcattctccc tgttcaaaac cgatgaggac
    14761 attacaggcc gtctgaaaga ccgtgtacag cccgaaatct tagaagcgct gttaaaacac
    14821 atcagcttcg acaagttcgt tcaaatttcc ttgaaagcat tgcgccgaat tgtgcctcta
    14881 atggaacaag gcaaacgtta cgatgaagcc tgcgccgaaa tctacggaga ccattacggc
    14941 aagaagaata cggaagaaaa gattgatctg ccacccatcc ctgccgacga aatccgcaac
    15001 cccgttgttt tgcgtgcctt atctcaggcc agaaaagtca ttaacggcgt ggttcggcgt
    15061 tacggctccc ccgcacgtat ccatattgaa acggcaaggg aagtaggaaa atcgtttaaa
    15121 gaccgcaaag aaatcgaaaa acgccaagaa gaaaaccgta aagaccgtga aaaagccgcc
    15181 accaaattcc gagagtattt ccccaatttt gtcggcgaac ccaaatccaa agatattctg
    15241 aaactgcgcc tgtacgagca acaacacggc aaatgcctgt attcgggcaa agaaatcaac
    15301 ttaggccgtc tgaacgaaaa aggctatgtc gaaatcgacc atgccctgcc gttctcgcgc
    15361 acatgggacg acagtttcaa caataaagtg ctggtattgg gcagcgaaaa ccaaaacaaa
    15421 ggcaatcaaa ccccttacga atacttcaac ggcaaagaca acagccgcga atggcaggaa
    15481 tttaaagcgc gtgtcgaaac cagccgtttc ccacgcagta aaaaacaacg gattctgctg
    15541 caaaaattcg atgaagaggg ctttaaagaa cgcaatctga acgatacgcg ctacgtcaac
    15601 cgtttcctgt gccaattcgt tgccgaccat atgctgctga caggtaaagg gaaaagacgt
    15661 gtctttgcct caaacggaca aattaccaat ctgttgcgcg gcttttgggg attacgcaaa
    15721 gtgcgtgcgg aaaacgaccg ccatcacgcc ttggacgccg tcgtcgttgc ctgctcgacc
    15781 gttgccatgc agcagaaaat tacccgtttt gtacgctata aagagatgaa cgcgtttgac
    15841 ggtaaaacca tagacaaaga aacaggagaa gtgctgcatc aaaaaacaca cttcccacaa
    15901 ccttgggaat ttttcgcaca agaagtcatg attcgcgtct tcggcaaacc ggacggcaaa
    15961 cccgaattcg aagaagccga taccccagaa aaactgcgca cgttgcttgc cgaaaaatta
    16021 tcatctcgcc ccgaagccgt acacgaatac gttacgccac tgtttgtttc acgcgcgccc
    16081 aatcggaaga tgagcgggca agggcatatg gagaccgtca aatccgccaa acgactggac
    16141 gaaggcgtca gcgtgttgcg cgtaccgtta acacagttaa aactgaaaga cttggaaaaa
    16201 atggtcaatc gggagcgcga acctaagcta tacgaagcac tgaaagcacg gctggaagca
    16261 cataaagacg atcctgccaa agcctttgcc gagccgtttt acaaatacga taaagcaggc
    16321 aaccgcaccc aacaggtaaa agccgtacgc gtagagcaag tacagaaaac cggcgtatgg
    16381 gtgcgcaacc ataacggtat tgccgacaac gcaaccatgg tgcgcgtaga tgtgtttgag
    16441 aaaggcgaca agtattatct ggtaccgatt tacagttggc aggtagcgaa agggattttg
    16501 ccggataggg ctgttgtaca aggaaaagat gaagaagatt ggcaacttat tgatgatagt
    16561 ttcaacttta aattctcatt acaccctaat gatttagtcg aggttataac aaaaaaagct
    16621 agaatgtttg gttactttgc cagctgccat cgaggcacag gtaatatcaa tatacgcatt
    16681 catgatcttg atcataaaat tggcaaaaat ggaatactgg aaggtatcgg cgtcaaaacc
    16741 gccctttcat tccaaaaata ccaaattgac gaactgggca aagaaatcag accatgccgt
    16801 ctgaaaaaac gcccgcctgt ccgttaaatt aatctatccc tgtttcaggc ggcctgaagc
    16861 agggattttt tatataaaaa taaggggaaa aacatgactt ggcgcagcct gcttattcaa
    16921 aacggcggca aactatcctt gcaacgccgg caactgctga ttcaacaaaa cggcgagtcc
    16981 cacaccgtac cattagagga catcgccgtt atcatcatcg aaaaccgcga aaccctgatt
    17041 accgctccac ttctgtcagc cttagccgaa catggcgcaa ccttactgac atgtgacgag
    17101 cagttcctgc cctgcggaca atggctgcct tatgcccaat atcaccgcca acttaaaatc
    17161 ctcaaactgc agctgaatat aagcgaaccc ttaaaaaagc aattctggca acatatcgtc
    17221 cgccaaaaaa tcctgaatca agcctttgtc gcagacgaaa ccggcaatga tcttgcagcc
    17281 aaacggctgc gcactttggc atctgaagtc cgctctggcg atacaggaaa ccgcgaagcg
    17341 taagccgccg ccctctactt ccaagccctc tttggcgaaa aatttacccg taatgacaac
    17401 aacgccgtca atgccgcatt gaactacacc tacgccgtgc tccgagccgc agttgcacgc
    17461 gccctgacct tatacggttg gttacctgca ttaggcttgt ttcatagaag tgaactcaat
    17521 ccatttaatc tcgccgatga ctttatcgaa cccttacgcc cattggccga cctgaccgtg
    17581 atacatttgt acgaacaagg ccgtctgaaa actgaactga cgctcggcat caaacaacac
    17641 ctcatcaaaa tcctctacta tcaaaccagc atcgaacggc aacatttcag caccttggct
    17701 gccatcgaca aaatggtttc gtcatttcaa gccggcgtaa ctgataaaaa cgccaaacaa
    17761 ctgaaactgc ctgaaatcct accattgaaa gcataccaat atgagtgagg ccaaatttat
    17821 gaggattatc gtcttcttcg acctaccggt tatcacggcg gcaaagcgca aagccgccaa
    17881 tcaattccgc cagtttttat taaaagacgg ataccaaatg ctgcaacttt ccgtatacag
    17941 ccgtatcgtc aaaggccgcg attcgttgca aaaacaccac aatagactat gtgcaaacct
    18001 tccgcaagaa ggctcaatcc gctgtttgga gataacagaa aagcaatatg ccgccatgaa
    18061 actgctgttg ggcgagctga aaacccaaga aaaaaaggtc aattcagacc aattattgtt
    18121 attttaagcc cattttttca taacaaataa aacgggaaac ccttatgaaa taaggatttc
    18181 ccgtcgaagt attgtagcac tgcgaaagga gaaagggagc tacaacacac cttgaacgca
    18241 aggcaatcgg aatgaaattg tagcactgcg aaaggagaaa gggagctaca acagtaaacg
    18301 ataagctcca ttgcgtgtgc gaattgtagc actgcgaaat gagaaaggga gctacaactt
    18361 aacaaaccac cccgtgtagg tgcgtggcat tgtagcactg cgaaatgaga aagggagcta
    18421 caacaaagaa agcgagcttg taaaaaggca gaagattgta gcactgcgaa atgagaaagg
    18481 gagctacaac caacgccttg cgtaaagtgt ttacgcgcat attgtagcac tgcgaaatga
    18541 gaaagggagc tacaactcgg tcaggtcata gacggctttg ttgtcgattg tagcactgcg
    18601 aaatgagaat gggagctaca acactcaaat tcagtctgtc aacccgttcc gcattgtagc
    18661 actgcgaaat gagaatgggc gctacaacca gccgttgcga taagcgaacg tgcgttaaag
    18721 tcattgagac acagtgcaat tatggcaaga aaggcgacag atattgtgtc acagatttga
    18781 aataagaaaa aaccgcccac tcacagagta ggcggaaaac ttgcgttttt ttgattgtgg
    18841 tcgggttagc tactgcattg acttaacagc aaagcaaccg ctaaaatgaa cttgtggttc
    18901 acttcaacgg acagcagcaa ctttttccca cgcataagga atttaagcat tttagcttct
    18961 cctagtgaag ttaatcacac aggcgagagc cgaacaaaaa tccccgttgc agcggggatt
    19021 tttgctgttc cctgtttgat gattaaacta aaatcttgca aaatgagacc tttgcaaaat
    19081 tccccaaaat cccctaaatt cccaccaagc catttagggg attttccatg agcaccttct
    19141 tccggcaaac cgcacaagcc atgattgcca aacacatcga ccgtttcccg ctattgaagt
    19201 tggaccaggt gattgattgg cagccgatcg aacaatacct gaaccgtcaa aaaaaccgtt
    19261 accttagaga ccaccgcggc cgtcccgcct accctctgct gtccatgttc aaagccgtcc
    19321 tgctcggaca atggcacagc ctctccgatc ccgaactcga acacagcctc atcacccgca
    19381 tcgatttcaa cctgttttgc cgtttcgacg aactgatcat ccccgattac agtaccttat
    19441 gccgctaccg caactggctg gcgcaagacg acaccctgtc cgaattactc
//
LOCUS       DAWFIE010000033        19427 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWFIE010000033 DAWFIE010000000
VERSION     DAWFIE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.033"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..477
                     /locus_tag="WGQ71_001086"
     CDS             <1..477
                     /locus_tag="WGQ71_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0707104.1"
                     /translation="NTKEVQRNNSNGTTWKELKTENQENGSFHAVSSLGLSAIYDFKL
                     NDKFDKFKPYIGVRVAYGHVKHQVHSVRKETTTVFSKPSGSTTKPGKIPSLVTKPAYH
                     ESNSISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYHF"
     gene            complement(451..660)
                     /locus_tag="WGQ71_001087"
     CDS             complement(451..660)
                     /locus_tag="WGQ71_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707105.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIGVGESEVVAHAQ"
     gene            complement(678..1235)
                     /locus_tag="WGQ71_001088"
     CDS             complement(678..1235)
                     /locus_tag="WGQ71_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0707106.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKQEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1311..2945)
                     /locus_tag="WGQ71_001089"
     CDS             complement(1311..2945)
                     /locus_tag="WGQ71_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ0707107.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3019..3348)
                     /locus_tag="WGQ71_001090"
     CDS             complement(3019..3348)
                     /locus_tag="WGQ71_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707108.1"
                     /translation="MFRCCLKRLQTASAVSAFSGGVLEVVRLCCRRLSVLFNPGVMLF
                     VPDCPVGGAVGFFQYEMLCPFFWQGRLQTGSNLAYDVFMSAGRLNGGRNPPHSRRFLA
                     LLCSVAL"
     gene            complement(3348..4454)
                     /gene="serC"
                     /locus_tag="WGQ71_001091"
     CDS             complement(3348..4454)
                     /gene="serC"
                     /locus_tag="WGQ71_001091"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ0707109.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFKTASEDLDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4819..5250
                     /gene="rimP"
                     /locus_tag="WGQ71_001092"
     CDS             4819..5250
                     /gene="rimP"
                     /locus_tag="WGQ71_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ0707110.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIGLGNIDKARLRPEFKF"
     gene            5278..6780
                     /gene="nusA"
                     /locus_tag="WGQ71_001093"
     CDS             5278..6780
                     /gene="nusA"
                     /locus_tag="WGQ71_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ0707111.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTAIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMNHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6792..9680
                     /gene="infB"
                     /locus_tag="WGQ71_001094"
     CDS             6792..9680
                     /gene="infB"
                     /locus_tag="WGQ71_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ0707112.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAGKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKTAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEASPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGENQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9891..11285)
                     /locus_tag="WGQ71_001095"
     CDS             complement(9891..11285)
                     /locus_tag="WGQ71_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ0707113.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVKNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLETHTDTRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLSGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(11359..12699)
                     /locus_tag="WGQ71_001096"
     CDS             complement(11359..12699)
                     /locus_tag="WGQ71_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ0707114.1"
                     /translation="MLNPSRKLVELVRILEEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIVCYGILPRLLAWVVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKDQ"
     gene            12842..13468
                     /locus_tag="WGQ71_001097"
     CDS             12842..13468
                     /locus_tag="WGQ71_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ0707115.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLVLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPSAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13532..14950)
                     /locus_tag="WGQ71_001098"
     CDS             complement(13532..14950)
                     /locus_tag="WGQ71_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0707116.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYPYEFHEFHDKG"
     gene            15277..16005
                     /locus_tag="WGQ71_001099"
     CDS             15277..16005
                     /locus_tag="WGQ71_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ0707117.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(16064..16552)
                     /locus_tag="WGQ71_001100"
     CDS             complement(16064..16552)
                     /locus_tag="WGQ71_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ0707118.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGTVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSVAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(17309..17542)
                     /locus_tag="WGQ71_001101"
                     /pseudo
     CDS             complement(17309..17542)
                     /locus_tag="WGQ71_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
     gene            complement(17678..17959)
                     /locus_tag="WGQ71_001102"
     CDS             complement(17678..17959)
                     /locus_tag="WGQ71_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ0707119.1"
                     /translation="MKELDKIDFRILKILQKNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGRDLCKNSLLTKFDA"
     gene            18184..19242
                     /gene="alr"
                     /locus_tag="WGQ71_001103"
     CDS             18184..19242
                     /gene="alr"
                     /locus_tag="WGQ71_001103"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ0707120.1"
                     /translation="MRPLNVQIRLGNLRHNYQILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGGDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTININTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
BASE COUNT         5142 a         5336 c         4870 g         4079 t
ORIGIN      
        1 aacacaaaag aggtgcaaag aaacaatagc aatggcacca cctggaaaga actgaagacg
       61 gaaaatcagg aaaacggcag cttccacgcc gtttcttctc tcggcttatc agccatttac
      121 gatttcaaac tcaacgataa attcgataaa ttcaaaccct atatcggtgt gcgcgtcgcc
      181 tacggacacg ttaaacatca ggttcattcg gtgagaaaag aaaccacgac tgttttcagt
      241 aaaccaagtg gtagcactac aaagccaggc aagatcccaa gtttggttac caaacctgcc
      301 tatcacgaaa gcaacagcat cagcagcttg ggtcttggtg tcatcgccgg tgtcggtttc
      361 gacatcacgc ccaagctgac cttggacacc ggataccgct accacaactg gggacgcttg
      421 gaaaacaccc gcttcaaaac ccacgaagtc tcattgggca tgcgctacca cttctgattc
      481 cccgacaccg atgccgtctg aaccttcaga cggcatgaga cctttgcctg cgtacttggt
      541 acgctggtcg cctccgaaca tggcgcgaca cccgacattt ccgccgaacg catcgggcgt
      601 ttcatgaatc cggtttaaaa cgcatggaaa aatgccgtct gaaagccttt cagacggcat
      661 tgtgcttgag attccgttta ccaatgactg acaaacgctt ccaaatcggt attcttgggc
      721 ttatgcactt cctctgtcgg cgtgccgacc atcatcagcc cgatgatttt atccttatcc
      781 gcacatccga aagcctcacg caacaggggg ctgttgaccc acatccccgt aatccagaca
      841 ttgtcgaaac cttgcgccgt tgccgccagt tgcagcgcat acgccgcaca acccgccgtc
      901 agcatctgct cccattcctg tttcggctta ggcacatcgc ggttcggcgc aaacgttacc
      961 ccgataacca tcggcgccat attgcccact ttttccgcct ttttcatcgc atcgtcgccg
     1021 aaattcaatt cggcaaccgt ttgcttcaac acatcgcgaa aacgttgcaa tcctacctcg
     1081 ccttgaatca cggtaaaacg gaaggggcgc atattgccgt gatcgggaac ttgggttgcc
     1141 gcctgaaata tttgttccaa ctccgccgca tcgggggcgg ggtgcttcag ctttttggaa
     1201 gatcggcggt tcgtcaataa ttttaaagca tccatatcgt tatactccgg tcatcggtcg
     1261 ggtgttcgga cacatatgcc gtccgaaggc ttcagacggc atatccggca tcagcgcgga
     1321 cggcggcagg ctgccaatat atccatttcc ttccgatagg tttggctatt ggaaatgtcc
     1381 atcagcccca ataccgtgct gaaatagtgg tcgtgcgaat attcgttttc cgccgctttt
     1441 tgtttgaggc attggaaatc tatgcccccg tgttggcgga aggctttgga aaaccacata
     1501 accatcggga tatgcgtctg cccggaaggc gcgatggcgt aaggcgcggc gtgcaggtac
     1561 atcccgtttt cgcccaaact ttcgccgtgg tcggaaacat aatgcaccac gctttccaaa
     1621 tcgtcgcggt tttcaagttt gcggataacc ttgtcgataa actggtccac atacaaaacc
     1681 gtattgtcgt aagtgttgac cagcgtggcg cgggtgcatt tgttgatttc gttggtgtcg
     1741 caggtcggcg tgaatttgcg ttcggcttcg gtatagcgtt cgtaatacgt cggcccgtgg
     1801 ctgccgatgg tatgcaggat taaaaccgcg tctttatcgt ttttgttgag gacttcgtcg
     1861 aacttagtca gcaggatatt gtcgaggcac tcgccgttgc ggcaatattc gggtaggttg
     1921 agcgaagtaa cgtcggtatt cggcactttg ccgcacacgc ccttgcagcc ggaatcgttt
     1981 tccaaccagg taacttccac accggcgcgc tgcacgatgt ccagcaggtt gtcttggtgt
     2041 tcggctttga tttcgtcata atccgtgcgg tcgaaggttg agaacataca cggcagggag
     2101 tgcgcggtcg atgtgccgca gcttctgacc tgcgggaaat tgacaatttc atcgccgcgc
     2161 gcggcaagca gcggcgtagt ttggcggctg taaccgttca aaccccagtt ggcggcacgc
     2221 gtggtctcgc ccacgaccaa aaccacgaaa cggcgcaggc tgccggccgg ccggttttgc
     2281 acgaccgcca tatccaattg cgtataagga atattggaac gcttccaatc tttgtatttc
     2341 gacacgcccg cgccgatgaa attagacggc acaatcagat gggttactga tttattgttg
     2401 cggaaaaacg aggcgtaatc ctgatattgc aacattgcga tacccaacgc gcacaaaaag
     2461 gaaacggcgg caagcacaag gcgtgtcaaa agctccttat accaaacgcg gtatttaacc
     2521 ttgacggcga tatacgccag cgcgggcaat acgcccaaac atacaatcca cagcacatag
     2581 cccggcgtaa tcaggcgcgc gctttcggca gccgtagttt gcaagacatt attcaacatc
     2641 gacttgttga aatagatatt gaaaaatatt tcttggtaag acaccgccgc actgataacc
     2701 aatatcaacg gaatcaatac cttatgcacg aaaggcaggg caatgacgtg aaaaacgaaa
     2761 ttacttaaaa aaaacagcac caccggcatc gtatagagga agatatccgc cccggtgccg
     2821 ttaaaaggat gaagctcgac aactttggca aaaaaggcgt aattcaatac cagcgaggaa
     2881 tacagggaaa ggaaggcaat cagcgcggaa gagccgagct tcggcctcag gttcggtttt
     2941 atcatttgga atgtgtcgga taagggttgg aaaaggcatc cggcatttgg aatccggatt
     3001 attgaaaaag attcttaatt ataaggcaac ggagcaaagc agggcaagaa aacggcggct
     3061 gtgcgggggg ttccgcccgc cattcaaacg tccggcagac ataaaaacat cgtaagcaag
     3121 attcgaaccg gtctgcaacc gcccctgcca gaaaaacggg caaagcattt catattggaa
     3181 aaacccgacc gcgccgccga cgggacagtc cggcacaaac agcatcacgc ccggattgaa
     3241 aaggacggat agccgccggc agcacaatct taccacctcc aaaacgccgc cggagaacgc
     3301 cgaaacagcc gatgccgtct gaagtcgctt cagacaacat cggaacatca accgtaacgc
     3361 cgttggaaat cgcgcataaa atctgccaaa gcccgcacgc cttcaagcgg catcgcatta
     3421 taaatgctgg cacgcatccc accgacagac ttatagccct tcaacaggca caagccctgc
     3481 aattcggctt ccaacacaaa gcggcggtcc aagtcttcgc ttgcggtttt gaagaccacg
     3541 ttcattttgg agcgcgcatt cggacggata cggttgatat agaaaccgcc gctgccatct
     3601 atcgtctcat acaaggtttg cgccttcagc cgattgaccg cttcaatttt tttcacaccg
     3661 ccctgcgcct gcagccagcg gaacaccagc cccgacatat aaatcgcgta agttgacggc
     3721 gtgttgtaca taccgtcgcg gttcaaatgc gaacggtagt tgaacacatc gggaatatcg
     3781 ttcggacaac gctcgagcaa atcctcacgc acaatcacca ccgtaactcc tgccggcccg
     3841 atgtttttct gtgcgcctgc gtaaatcagt ccgtagtcgg caacatcaaa ctcgcgcgac
     3901 aaaatctcgc tggacatatc gcacaccagc ggcggcatgc cttctgaaag gcgcggcact
     3961 tcacggtatt gcagcccgtt gaccgtttca ttgacggcaa aatggacaaa cgccgaatcg
     4021 ggtgcaacat cccacgtttc cacaggcggc aggtcgagat agtcgaactg ctcgccgcca
     4081 tgcgccgcca aacggatttc cgtatcggtc aaacggctca tctgttcata agcgatacgg
     4141 ctccagttgc ccgttaccac cgcgtcggca gtgcggaaac cgtgtgccag attcatggct
     4201 gccatattaa attgggttgt tgctccgccc tgcagaaaca atatcttata gttgtcaggc
     4261 actttcaaaa gctgcctcaa atcctgttcc gcatgatgca ggatgctcaa aaacatttcc
     4321 gaacggtggc tcatcgccat cacgggaaaa cccgtaccgt tgtagtccaa catttcctgc
     4381 cgcgccgttt ccaacacggc ttcgggcaat acggcagggc cggcggaaaa attgtaaatc
     4441 ggataaagag acatgatgca gccttgattc tgaacaataa cccgcctgat tttaggcttg
     4501 cagcaggctt tgtgcaagga tgggaaatac ctgtcctccg cccgattcca tgccgcccga
     4561 acacggaaaa caacatcaat atattgattt acaaacataa aaatcatgct agcgacaaat
     4621 agatacattt gttttgtcaa caatattcac gatttcccat tacaaacctc ccttacaccc
     4681 gcttttttcc gtcccaaaaa cacaaaataa atcaacactt tcatttctcc gcaaaagcgg
     4741 ttataattac gccgattttt caactttgac gaaaaatggc cactgggcca tttttttatt
     4801 atgtacatag ggagcagcat ggatattcaa accatcctcg aaaaaaccct gcccggcctg
     4861 ggctacgaac tggtcgattt cgaactgacc gcgcaaggaa cattgcgcgt gttcatcgac
     4921 aaagaaagcg gcattaccgt cgaagactgc gcaaccgtca gcaaccactt gagccgcgtc
     4981 ttcatggttg aagacatcga ctacaaaaac ctggaaattt ccagccccgg actcgaccgc
     5041 cccttgaaaa aagccgccga cttcgtgcgc tttgccggtc agaatgccaa aatcaaaacc
     5101 cgcctgccga tagacggtca gaaaaacttt atcggtaaaa tcgaaggctg cgaaaacgat
     5161 accgttaccg tatccttcga cggcaaaacc gtacaaatcg gattgggcaa catcgacaaa
     5221 gcccgtctgc gccccgaatt caaattctaa aacacaacaa tattggagat gttcaaaatg
     5281 agtcgtgaaa tgttacagct ggcagaagca ctggcaagcg aaaaaaacgt tgatgcggaa
     5341 gtcgtcttcc aagcactgga attcgccctg tctaccgccg ccaagaaaaa ggcagaccgc
     5401 gaacacatgg acgtgcgcgt ccaaatcaac cgcgacaccg gcgaatacca aaccttccgc
     5461 cgctggctga ttgtcgccga tgaagactat acctatcccg atgtcgaaaa aaccatcgag
     5521 gaaatccaag aggaaattcc cggcaccgcc atccaaatcg gcgaatacta cgaagagcag
     5581 ctgcccaacg aaggcttcgg ccgccaagcc gcgcaaaccg ccaaacaaat catcctgcaa
     5641 cgcatccgcg atgccgagcg cgagcagaat ctgaacgagt ttctcgccgt caaagaagac
     5701 atcgtgtccg gcacggtcaa acgcgtcgaa cgccacggca tcatcgtcga agtcgttgcc
     5761 ggcaaactgg acgcgctgat tccgcgcgac caaatgattc cgcgcgaaaa cttccgcagc
     5821 ggcgaccgca tccgcgccct ctttctgcgc gtcgaagaaa tcggcaacac cggccgcaaa
     5881 caagtcatcc tgagccgtac ttccggcgat ttcctcgtca aactgtacgc caatgaagta
     5941 cctgaaattg cagacggcat gcttgaaatc cgcgctgtcg cccgcgaccc gggacaacgt
     6001 gccaaagtcg ccgtcaaagc caacgaccag cgcatcgatc cgcaaggcac ctgtatcggc
     6061 gttcgcggtt cgcgtgtcaa tgccgtcagc aacgaattgt ccggcgagcg catcgacgtt
     6121 gtcctttggt cgcccgagcc tgcccaattc gtcatgagcg cgctctcacc cgccgaagtc
     6181 agccgcatcg tcatcgacga agacaaacac gccgtcgatg tcatcgttgc cgaagaccag
     6241 ctcgcgctcg ccatcggacg cggcggtcaa aacgtgcgcc ttgcttccga cctgaccggc
     6301 tggcagctca acatcatgac ttccgccgag gcagacgaac gcaatgcggc agaagatgcc
     6361 gccatccgcc gcctgtttat gaatcacttg aacgtggacg aagaaaccgc cgacgtactg
     6421 gttcaggaag gttttgcaac cttggaagaa gtcgcctatg ttcctgccgc cgaactgctt
     6481 gccattgaag gatttgacga agaaatcgtc gatatgctcc gcaaccgcgc ccgcgatgcc
     6541 atcctgacca tggcgattgc cgccgaagaa aaactgggcg aagtatccga cgatatgcgc
     6601 aacctcgaag gcatagatgc cgatatgctc cgcagccttg ccgaagcagg cattaccacc
     6661 cgcgacgact tggcagagct tgctgtggac gaactgattg aaatcaccgg tgtaaacgaa
     6721 gaaaccgcaa aagccgtcat cctgaccgca cgcgaacact ggtttaccga agacaaataa
     6781 agggggtaca gatgagtaac acaaccgtag aacaatttgc cgccgagctg aaacgccccg
     6841 tcgaagacct gttgaaacag ttgaaagaag ccggcgtcag caaaaacagc ggcagcgatt
     6901 ccctgacgct ggacgacaaa cagcttctga acgcctacct gaccaagaaa aacggcagca
     6961 acggcggcac catcagcatc cgccgcacca aaaccgaagt cagcaccgtt gacggcgtaa
     7021 aagtcgaaac acgcaaacgc ggacgcactg tcaacattcc ttctgccgaa gaattggcag
     7081 cacaggtaaa agccgcccaa acccaagccg cacctgtccg gccggagcag acggcagaag
     7141 acgcggcaaa agcccgagcc gaagctgccg cacgtgcaga agcccgtgcc aaggcagaag
     7201 cggaagcggc aaaactgaaa gcggcaaaag caggcaacaa agccaaacct gccgcgcaga
     7261 aacccaccga agcaaaagcc gaaaccgcac ccgttgcggc ggaaaccaaa cccgccgaag
     7321 aaagcaaagc gggaaaagcc caagccgaca aaatgccgtc taaaaaaccc gccgagccca
     7381 aagaaaaagc cgccaagccg aaacacgagc gaaacggcaa aggcaaagat gccaaaaaac
     7441 cagcgaaacc tgccgcacct gccgtgccgc aacccgtggt cagcgcagaa gaacaggcgc
     7501 aacgcgatga agaagcacgc cgtgccgccg cacttcgcgc ccaccaggaa gccctgttga
     7561 aagagaaaca ggaacgccag gcacgccgcg aagccatgaa acaacaggca gaacaacagg
     7621 caaaaaccgc acaggaagcc aaaaccggca gacagcgtcc cgccaaacct gccgagaaac
     7681 cgcaggcagc cgcgccggcc gtcgaaaaca agcctgtcaa tccggcaaaa gcgaaaaaag
     7741 aagaccgccg caaccgcgat gacgaaggtc aaggccgcaa cgccaaaggc aaaggcggaa
     7801 aaggcgggcg cgaccgcaac aatgcacgca acggcgacga cgagcgcgta cgcggcagca
     7861 aaaaaggcaa aaaactcaaa ctcgagccga atcaacacgc cttccaagca ccgaccgaac
     7921 ctgtcgtaca cgaagttttg gtgcctgaaa ccattaccgt tgccgacttg gcacacaaaa
     7981 tggcagtcaa aggcgtggaa gtggtcaaag ccctgatgaa gatgggcatg atggttacca
     8041 tcaaccaatc catcgaccaa gacaccgccc tgattgtcgt cgaagaactc ggacacatcg
     8101 gcaaacccgc cgccgccgac gatcccgaag ccttcttgga cgagggcgcg gaagcggtgg
     8161 aagccgaagc attgccgcgt ccgcccgtcg ttaccgtcat gggccacgtc gaccacggca
     8221 aaacctcgct gctggactac atccgccgta ccaaagtggt acagggcgaa gcaggcggca
     8281 ttacccaaca catcggtgca taccacgttg aaaccccacg cggcgtgatt accttcttgg
     8341 atactccggg tcacgaagcg tttactgcca tgcgtgcgcg cggtgcgaaa gcaaccgaca
     8401 tcgtgattct tgtggtcgcc gccgacgacg gcgtgatgcc gcaaaccatc gaagcgattg
     8461 cccacgccaa agcggcaggc gtaccgatgg tggttgccgt caacaaaatc gataaagaag
     8521 ccgccaaccc agagcgtatc cgccaagagc tgaccgcaca cgaagtcgtg cctgacgaat
     8581 ggggcggcga tgtacagttt atcgacgttt ccgctaaaaa aggtctgaac atcgatgcat
     8641 tgctcgaagc cgtattgctc gaagccgaag ttttggaact gaccgcacct gtcgatgcgc
     8701 ccgccaaagg cattatcgtc gaagcccgtt tggacaaagg gcgcggcgcg gttgccacat
     8761 tgctggttca aagcggcacg ctgaaaaaag gcgatatgct gctggccggt accgcgttcg
     8821 gcaaaatccg cgcgatggtc gatgaaaacg gcaaatccat taccgaagcc agcccgtcca
     8881 tccccgtcga aatcctcggc ttgtccgacg taccgaatgc gggtgaagat gcgatggtat
     8941 tggcggacga gaaaaaagcg cgcgaaatcg ccctcttccg ccaaggcaaa taccgcgacg
     9001 tgcgccttgc caaacagcag gcggcgaagc tggaaaatat gttcaacaat atgggcgaaa
     9061 accaagccca atctttgtcg gtcatcatca aggcagacgt gcaaggttct tacgaggctt
     9121 tggcgggcag cctgaaaaaa ctgtccaccg acgaggtgaa agtgaacgtg ttgcacagcg
     9181 gcgtgggcgg cattaccgaa tcggatgtca accttgccat cgcttcgggc gcgttcatta
     9241 tcggctttaa cgtgcgtgca gatgcctctt cgcgcaaact tgccgaaaat gaaaacgtgg
     9301 aaatccgcta ctacaacatc atctacgatg ccatcgacga cgtgaaggcg gcgatgagcg
     9361 gcatgctttc tccggaagag aaagaacagg ttaccggtac ggtcgaaatc cgtcaggtca
     9421 tctccgtttc caaagtcggc aacattgcag gctgtatggt taccgacggc gtggtcaaac
     9481 gcgattccca tgtccgcctc atccgcaaca acgtagtcat ccacacgggc gaactggctt
     9541 cgttgaaacg ctataaagac gacgtcaaag aagtccgtat gggcttcgag tgcggtctga
     9601 tgctcaaagg ctacaacgaa atcatggaag gcgaccaact ggaatgcttc gacatcgtcg
     9661 aagtggcacg caccctgtga ttttgatgcg gaaaaatgcc gtctgaagcg ttcagacggc
     9721 atacggaacg ggttctgtat catacagaac ccgtttttta tcgcaaatcg gcttcagaca
     9781 gccctcctgc cttatcccga tttgaatctg acttgccata caaacaggct tcagacggca
     9841 ttatagtgaa ttaaatttaa accggtacag cgttggctcg ccttgccgta ctatttgtac
     9901 tgtctgcggc tcgccgcctt gtcctgattt aaatttaatt cactatattt tcccgacagg
     9961 cggatttgaa gtttctccac atatccggcg cgttcggcgc ggctggtttc ggggcggtgc
    10021 gtattgagcg acgaccattt ccaatgactg cgggctttgt tgagttcggg cgggagtctg
    10081 gcggcgtccc acggggcttt gcggctgtgc agctcgatat ccgactgtgc cgcgtgtccg
    10141 cgcgtttgca ggacgtggag caaatcgagg gcgcgggcgg cgagcagggt cagggtttca
    10201 gggtcggtgt gcaaggtttg gcggccggag agtttgtcgg aaatggtgcg ggtattgggc
    10261 aggatgccgc ccaaaaagcc gccgattgcc gtacccaatc cgagcgagcc gccgagtgtg
    10321 gcgatgtcca gccccaagcc gatgagcgcg ccggttgccg cgcccgtgcc ggtgcggatg
    10381 ccgtattgtt tgagcaattc gctgtcgaac gggtcttggc ggaaggcttg cggcatccag
    10441 tcgccgccgt cgatttcgct gtggtagaaa cggtagaggg caaacagccg ctgctgcatc
    10501 tgccgttcga gttggcgtat ttccgcctgc atggtttgca gcacggtggc ggtatcctcg
    10561 ttttcgtcca cttcctgcct gaaggcggcg gcatcgagca ggaaatcggc gatttcgcgg
    10621 cgcgcttcgc cgtccagccg ctgccattcg cgccggcgca tggctgtcag gcggtcaagt
    10681 gtgctgcgtt cgggcaacat ggtggcgagg ttttcccaca ggcgtagttc gccttcaaaa
    10741 tcaaaggcga cggtgtcgaa ccctgcgaaa acgtgcaggt ttctcctcgc cagcatggtt
    10801 gtccacgatt cgggaagctg tccgccggta aagttgaaca cgggcataac cggtttggca
    10861 caccatgaaa ggatggtcaa ttcatcccga tatttgtcga ggacgggttc gcgcgcgtcg
    10921 atgacgtaca ttgccatatc gctttgcaag acttgccgta agactttggc ttcctgattg
    10981 aaatcatggt gcgcaccgtg gctgccgaga aactgttgca gccgctcgat gccgtctgaa
    11041 cgcgtatccg tatgggtttc cagccattcc aaaacaccgc ccgcgtcttc gagtccgggc
    11101 gtgtcataca ggaaaaccag catgtccgcg ccgtcgctga tggcggcttc ttcgacatgg
    11161 cgcgtagtgg acggggcatt tttgacttcg ccgaagccgc tgtcgcgcaa tagggtgcgc
    11221 aggagcgagg ttttgccggt attggtatgc ccgacgacgg cgagggaaag gggttgtttg
    11281 ttcatgatgt ttttgaagaa tggattttca gacggtcttt tttcagaatg gcggcttaac
    11341 agaacatttc aagtgagttt attggtcttt caaacgccct tcctgcgccg ccctgtcagg
    11401 ctcaagccac gccgcgccgc attcggccag cgcgttacgc caatgttcca gcttttccga
    11461 aaggtcgtct gaaagcccct gttccgccaa aagctgcacc accgcgccgc cctgcgccgc
    11521 ttccgaaagt cggacgatct gccgcaacac gccgcggtcg ggcacagttt gggcgcgcac
    11581 gccgataagc agttgcgccg gtttctgctt cagctctgtc tccagcgcgg caacctgttc
    11641 ccgattggcg gcaacgccct tatccagcca ttcctgcgcc agcctgccct cgaaccattc
    11701 gccgtcctgc cattcggtct ccagcatgac cgcccatttc ggcgcatcgt tcaagacgat
    11761 tttcggcgaa acggcggaca cggtttcccg acgcgtatcc gcatcggtga ttttgttctg
    11821 ccagcggcgg atgaccgcct gataataggg cttttccaaa tccaagccgt tttcgcttgt
    11881 tttcaaaagg attttacaca ctacccaagc caagaggcgc ggcaggatgc cgtagcagac
    11941 gatactgccg accagcagcc ccgaccaagc ccgcgcatcg gcgatgttgc cgttcagacg
    12001 gccttcgatg accgcccgcg catcaggcac gggaaaaccc agtttcgcag gcagccatgc
    12061 caacatttcc accagccgta ccgaagacga atcgcccaac agcgtgcttt cccagttgaa
    12121 cgtatattgc cgcaccaaaa gcagcaacaa taccgacacc agcattccga gcagcgtgca
    12181 gagccacagg ctgtgcgacg ttgcgcctat tttccaacgt accgaaggtt gccgccactc
    12241 gtccgcatac agccgcaaca ccgcctgatt tacagggtct ttgccccgaa accacgtcgc
    12301 cggactgctg aaaaaacgcc ccactttcac gcgcaggaac aacattgcca accatactgc
    12361 cagcatcagc gtattcatgc ccaacacgcc cgccaaaacc aaaaagaaat tcagaccctg
    12421 attgtccatt agaagataag tacctgaaaa accggtaaaa aacgcaaacg tcgccgccgc
    12481 cacccacaac cagaacgacc ccgcacgcac acgttccaac gtgtcccgca gcatacggtt
    12541 cctgtcgatc atctccgccc gacggatgat tttttcctcc gtactgccgt ccacgcggcg
    12601 caaagcctcc gtcgcctgca cgggatcgcc gctgaaaata aagccgcctt cttccaaaat
    12661 acggaccagc tcaaccagtt ttcgggatgg attcaacata aaatgccgtc tgaaaataaa
    12721 aaacagattt taacacacga attttcaaga atattcacag tgtaggcaaa gagtaaatcc
    12781 cacacagagg caaaagtatc ggcgtaaact gactgcctct actttcccga aagattgtgc
    12841 gatgtataca ggcgaacgct tcaatactta cagccatttg agcggtttga ttctggcggc
    12901 ggcaggtttg gtgctgatgc tgctgaaaac cataggacac ggggacggct accgtatctt
    12961 cagcgtatcg gtttacggca tcagccttct tctgctctat ttgagttcct cgctgtacca
    13021 cggaattgca gccggaaaac tgaaaagcat tttgaaaaaa accgaccact gcatgattta
    13081 tgtcctgatt gccggaagct acacaccgtt tgcactggtt tctttgagaa acgggccggg
    13141 ctggacggta ttttcactgt cctggctgct ggcggctgca ggaatcgcac aagaactcac
    13201 catcggacgg aaaagcgaaa aacgtctgct gtctattgtg atttatgtcg tcatgggttg
    13261 gatggtcttg gcggtaatga aatccctgac agcctcactc ccgtcggcag gactggcttg
    13321 gctggcggca ggcggtatgc tgtacagcgt cggcatttac tggtttgtaa acgatgaaaa
    13381 aatccgacac gggcacggaa tctggcatct gttcgtattg ggcggcagca tcacccaatt
    13441 tgtcagtgtg tacggttatg taatctgaat gccgtctgaa aaacaaaacc tcccgatcct
    13501 gaagattggg aggttttctg tttgccggac atcagccctt gtcgtggaac tcgtggaatt
    13561 catacggata agacaaatcc cgacccgctt ttttctgtgc caaataatca tcataaatgg
    13621 cacggatttc cttacgcaac aaaaacaggg ctatcaagtt cgggataacc ataaagccgt
    13681 tgaacatatc ggacaggctc caaaccaaat cgactttgcc gagcgtacct aaaacgatgg
    13741 caagcagaac caatgcgcga tagatgccca agtgtcttcc cctgaaaaga aaacggatgt
    13801 tggactcacc gaaataatac cagccgataa tggtcgtcaa ggcgaagaag gtcagacaca
    13861 cggcaagcaa ttgcgaaccg aagcccggaa atgccttgtt aaaggcaaat tgagtaaccg
    13921 ccgcgccctg ttcgcccgaa aggttggcat cggtcagcag gataatcagc gcagttgccg
    13981 tacataccaa aatcgtatcg ataaacacac cgacaaatgc cgccatacct tgctgcacag
    14041 gatgcttcac atccgcagtc gcgtgggcgt gcggagtcga acccatcccc gcctcattgg
    14101 aaaacagacc gcgcgccacg ccgaaacgta tcgcttcgcg cataccgata cccgccgcac
    14161 cgcccaaaac ggcttcggga ttgaaggcgg cggtaaagat gtggttgaac atcggcacaa
    14221 tatggtcgga aaattcaaac aggataacga cggcgcacaa aatataaaca accgccataa
    14281 acggcacgac aaattgggca atattggcaa tacggttcac gccgccaatc acaaccatgc
    14341 ccgcaaggac ggcaagcaca ataccgactg ccaaagaagg cacatcaaat gcaatggtaa
    14401 cggcagaagc aatggagttt gcctgtgtcg cattaccgat aaagcccaat gcgataatca
    14461 acgcaatgga aaagaaaccg gacaaaaaac gcgccgctcc cctgccgatt ttcggagtca
    14521 gaccgtgggt gatgtagaac gccggcccgc cgatgtattt gccgtggctg acgacgcggt
    14581 atttctgcgc cagcagtgcc tccgcaaaaa tcgtggacat ccccaaaacg gcagaaaccc
    14641 acatccaaaa aatcgcgccc ggcccgcctg cggtgatggc ggtcgccacg ccggcgacgt
    14701 tgcccgtacc gatttgcgcg gatatggcaa ccgccaacgc ctgaaactgc gataaagact
    14761 tgtcgtcttt atcgcctttg gcaaacaagc cgccgaatac ggatttgaat cccgcgccca
    14821 gcttggtaat ctgcggcgca ccaagataca gcgtaaaaaa caggccgata cccaaaagcg
    14881 cgtaaatcag caggtagtcc caaaggaacc gattgactgt acccaccaga acagacaata
    14941 tattttccat aaaataaacc ttatcttaca attaaaatga ctgccttcca aaagacattc
    15001 caataaggaa acacggcgag cagaccgtat ttgccgcaac agatgcctta aattgtcaac
    15061 aatcggggag aagctgcgcc atcataccgt aaaatatcgt taattaaaac atttctttat
    15121 ttttaagcgg aaagcggagg aaatcgcttt cagacagcat agacaacggc acggcataaa
    15181 acaggatatt ttgggtactt gcaacttatg ttaaaatgcc gaccgtaaaa aatctgacaa
    15241 aaacagatta attatttgaa ataagaaagg aaatttatgg caggccatag caagtgggcg
    15301 aatatccagc ataaaaaagc ccgtcaggat gccaaacgcg gcaaaatctt cacccgttta
    15361 atcaaagaaa tcaccgttgc ggcgcgtatg ggcggcggcg accccggtgc caacccgcgc
    15421 ctgcgcctgg ctttggaaaa agcagccgaa aacaatatgc ccaaagacaa tgtgcaacgc
    15481 gccatcgata aaggtacggg caacttggaa ggcgtggaat acatcgagtt gcgctacgaa
    15541 ggctacggca tcggcggcgc ggctttgatg gtggactgcc tgaccgacaa caaaacccgc
    15601 accgttgcgg acgtacgcca cgcgtttacc aaaaacggcg gcaacttggg taccgacggc
    15661 tgcgtggcgt tcaacttcgt gcatcagggc tatttggtat tcgaacccgg cgttgacgaa
    15721 gacgcgctga tggaagcggc tttggaagcc ggtgcggaag acgtggttac caacgacgac
    15781 ggttccatcg aagtcattac cgcgccaaac gattgggcgg gcgtaaaatc cgctttggag
    15841 gcggcaggtt acaaatccgt tgacggcgac gttacgatgc gcgcccaaaa cgaaaccgaa
    15901 ctctccggcg acgatgccgt caaaatgcaa aaactgattg acgcgctgga agatttggac
    15961 gacgtgcaag acgtttacac ttccgccgta ttgaatttgg actgatacgc agcacagcag
    16021 acatacaata aaatgccgtc tgaacctttc agacggcatt gatttattta gcccttgccc
    16081 acgcccacca aaccgtcagg acaaccgcca gaaaatacgc cacactccaa acaggcaaag
    16141 caacccccag cagataatcc ggttcggcac aatttccgaa cccgcgcacg acaggctcga
    16201 accaatcaaa caaaggccag cccttcaagc ggaacgtcca cggcgcaccg cacgaaggag
    16261 ccgtacccgg cggcagcgac tgcaaccaca actgatatgc cgcaacagaa atacccgtaa
    16321 cggccggaat gctgataaag acagtaccga acaaaccgcc cgcccttctt cttggtctgc
    16381 acatcaggac aattgccgta cacaatgcgg ttgccaaaac gcataaccgc tgactgatac
    16441 acaaaacgca aggctccata cccaaaacat actgtgccgc caaagaaccg gcaaatgcac
    16501 agaccgaaac ggcaaacagc agccaaacgg cttttctaaa taacggggtc attttctcaa
    16561 cacaccaatc aaaataccga tatgccgatt ttgctggata tatcccgaga tggcaaggga
    16621 cgaatcgaca ccagacaggt gcggacgatt tcggttttcc cgatatgcac aagacctaac
    16681 aagggacaaa acggcgattt gcccgcacaa tgcccgcaaa aaaatacatc tagaggattt
    16741 gaatttcggc aaatttagcg aatcaaaagt ttatttcaat gaaatcatat gatttttttg
    16801 aataagcgga ttgatgggtt tttgaaggaa tttgttactg gatagccatc gggcaagttt
    16861 tttgcaaaat ttgaatcggt cgggtaaatt ttcaaaaaat aattgacagc ggataagaaa
    16921 cggcggataa ttcccgccgt cgagttgctt gatgcagctt gatttttctc ctctatttct
    16981 cctttgtaga cttggcacac attcaactgg atgtgtgcat ttttttatct ccgctttttt
    17041 tgaaatttaa ttacttttat tgtttgaaat tccattcttt aagaaattta acaagaaaaa
    17101 cgcttgcttt ttgaccggaa aagctgcata attcattccg ttagctggtt cgagtagtca
    17161 gttaatagtt tctcctctat ttctcctttg tagacttggc acacattcaa ccggatgtgt
    17221 gcattttttt atctgaagca acaagcctct gtgcgtgatg ttgttatgtt tcatttaggt
    17281 gtcaaaccgc atagccagcc tgaaatattc aatccaaatc caaaaccgga ttttctttga
    17341 cctcctccat cacaacataa ctcctactct ccgaagcggc aggcaattgc aataggatat
    17401 tgcctagcat atcccgatag gcagacatat cgggcaaacg tactttaatc agatagtcgt
    17461 attcgcccga caccaagtgg cattccataa tttgcggaat tttcagcact tcttttttga
    17521 aatcttcgaa aatattgccc gagacctttg caataacata ggttactaaa attttatgct
    17581 caatctcatt ttcaaaatgc aaaacttttc tgatttttcc tactttttgc tcaatattag
    17641 gaaggtttta ggcaattgaa aattttttgg cgcattttta tgcgtcaaat ttcgttaaca
    17701 gactattttt gcaaaggtct ctgcccgatt tggattgcag cttcagctcg acaaaaacca
    17761 ataaaggttt gcccagcagg tggggattga gatgggcgtg ataaccggaa atataatgtt
    17821 cccgctccaa acggcgtacc cgttcggtaa ccggggtagt ggacaatcct actttctcgg
    17881 caagctccgt catgggaata cgggcatttt tctgcaggat cttgaggata cggaaatcaa
    17941 tcttatctaa ctctttcatt tgtctttacc ttattgttta ttattgattt taatggtttg
    18001 gatgctgata aggaaagaca attatatcag gaaaacacaa gcctcctttc cctatggcaa
    18061 acccaacctc acctccggag agtaaggttc tttaatattt taaaagcctt gccgttggat
    18121 tataattccc taattgattt cttaattttg ctaataaaca cttgtttggt aaggaatgaa
    18181 tttatgcgcc ctttgaacgt gcagatcagg ttgggcaacc ttaggcacaa ttatcagatt
    18241 ttaaaggaaa tgcacggagg caaactgttg gcggtagtga aggccgacgc atacggacac
    18301 ggtgcggtca gatgtgcttt cgcgctggca gacttggcag acggttttgc cgtggcgaca
    18361 atcgatgaag gaatcaggct gcgggagagc ggcattaccc atccgattgt ccttttggaa
    18421 ggcgtatttg aagcatcaga gtacgaagcg gtcgaacaat actcgctctg gccggcagtc
    18481 ggaaaccaat ggcaacttga ggctttgctt tcccgccatt ggaaaaaacc tgtcaaggtc
    18541 tggttgaaaa tggattcggg aatgcaccgt accggttttt tccctcatga ttacgcttcg
    18601 gcatatgcgg cattgaagca gtcggaatat gtggacagta ttgtcaagtt ttcgcatttt
    18661 tcctgtgcgg acgaacccga aagcggtatg acggaaatac agatggaagc attcgatttg
    18721 ggtacggaag ggctggaagg cgaagaaagc cttgccaact ccgccgctat tttgaatgtt
    18781 cccgaagcac gcagggactg ggggcgcgcc ggtctggcgt tatacggcat ttccccgttc
    18841 ggaggaggcg atgacaggct gaagcccgtg atgaggcttt ccacccgtat tttcggcgaa
    18901 cgcgttttac agccgcactc ccctatcggt tatggcgcaa cattttatac cagcaaatct
    18961 acgcgcgtcg gtctgattgc ctgcggttat gcggacggtt atccgcgccg cgccccaagc
    19021 aattcccccg tcgccgtcga cggcaaattg acccgggtca tcggcagggt ctctatggat
    19081 atgatgacca tcgagctgga cgcttcgcaa gaaggtttgg gacacgaggt cgaactgtgg
    19141 ggcgatacga tcaacatcaa taccgttgcc gaagcggccg gaaccatccc ttacgaattg
    19201 atgtgcaata tcaaacgtgc aaaattcact tatatcgagt aatcgagtcc aaacaaaaat
    19261 gccgtctgaa gcctttcaga cggcataaat gcgttttgac aaaatcagta tagtggatta
    19321 acaaaaatca ggacaaggcg acgaagccgc agacagtaca gatagtacgg caaggcgagg
    19381 caacgctgta ctggtttaaa tttaggggct gtcctagata actagga
//
LOCUS       DAWFIE010000034        18998 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWFIE010000034 DAWFIE010000000
VERSION     DAWFIE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18998
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.034"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            45..1223
                     /locus_tag="WGQ71_001104"
     CDS             45..1223
                     /locus_tag="WGQ71_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ0707121.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASV
                     KYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAADTVVWNGPVGVFEFDQFAGGTKALTEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGE
                     "
     gene            complement(1291..1539)
                     /locus_tag="WGQ71_001105"
     CDS             complement(1291..1539)
                     /locus_tag="WGQ71_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0707122.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWNAKAQ"
     gene            complement(1630..2547)
                     /gene="ftsX"
                     /locus_tag="WGQ71_001106"
     CDS             complement(1630..2547)
                     /gene="ftsX"
                     /locus_tag="WGQ71_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ0707123.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLVRDKRLDNIRFISKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIA
                     LVFGFVIALGIFGAWLATTQHLLGFKAKK"
     gene            complement(2544..3194)
                     /gene="ftsE"
                     /locus_tag="WGQ71_001107"
     CDS             complement(2544..3194)
                     /gene="ftsE"
                     /locus_tag="WGQ71_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ0707124.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPSLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(3389..3871)
                     /locus_tag="WGQ71_001108"
     CDS             complement(3389..3871)
                     /locus_tag="WGQ71_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0707125.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTEANSRNFMKSYGNNVGVLPFTVVEAPKCGYKQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            3995..4348
                     /gene="arsC"
                     /locus_tag="WGQ71_001109"
     CDS             3995..4348
                     /gene="arsC"
                     /locus_tag="WGQ71_001109"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ0707126.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPNLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            4345..5001
                     /locus_tag="WGQ71_001110"
     CDS             4345..5001
                     /locus_tag="WGQ71_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ0707127.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            5117..6511
                     /locus_tag="WGQ71_001111"
     CDS             5117..6511
                     /locus_tag="WGQ71_001111"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ0707128.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLNYDNADNVVYQTRRFDRYKEVIAELLEKGHAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTRWADL
                     VKGEISIPNEALDDLIIARSDGSPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            6532..6927
                     /locus_tag="WGQ71_001112"
     CDS             6532..6927
                     /locus_tag="WGQ71_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707129.1"
                     /translation="MPSDVGIRLQTASKRKLNMKKILYFLMVVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            6931..7422
                     /locus_tag="WGQ71_001113"
     CDS             6931..7422
                     /locus_tag="WGQ71_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0707130.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDFGTLLGSAAVKRHDMKSFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            7467..7577
                     /locus_tag="WGQ71_001114"
                     /pseudo
     CDS             7467..7577
                     /locus_tag="WGQ71_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7651..10245)
                     /gene="mutS"
                     /locus_tag="WGQ71_001115"
     CDS             complement(7651..10245)
                     /gene="mutS"
                     /locus_tag="WGQ71_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ0707131.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLAALRDSLFALSEIDLSANGSSLLETLKAVFP
                     ETLPVAETLKVAVMPEPAVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPKF
                     ADYPAIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAALNMHL
                     SALEQGRDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELENQAAANRP
                     QLDIFSTMPSEKGDELNVGNFVDKAEEKHFEGILATALENLDPDRLTPREALSELYRL
                     KDLCKSVS"
     gene            10687..11691
                     /gene="gap"
                     /locus_tag="WGQ71_001116"
     CDS             10687..11691
                     /gene="gap"
                     /locus_tag="WGQ71_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0707132.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(11761..11880)
                     /locus_tag="WGQ71_001117"
                     /pseudo
     CDS             complement(11761..11880)
                     /locus_tag="WGQ71_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11949..12629)
                     /gene="tsaA"
                     /locus_tag="WGQ71_001118"
     CDS             complement(11949..12629)
                     /gene="tsaA"
                     /locus_tag="WGQ71_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ0707133.1"
                     /translation="MTYTIVPIATARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEHFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQDIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            12744..13379
                     /locus_tag="WGQ71_001119"
     CDS             12744..13379
                     /locus_tag="WGQ71_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ0707134.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHAAAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPCAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            13416..14384
                     /gene="waaC"
                     /locus_tag="WGQ71_001120"
     CDS             13416..14384
                     /gene="waaC"
                     /locus_tag="WGQ71_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ0707135.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            14611..15360
                     /locus_tag="WGQ71_001121"
     CDS             14611..15360
                     /locus_tag="WGQ71_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ0707136.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDAKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            15371..16306
                     /locus_tag="WGQ71_001122"
     CDS             15371..16306
                     /locus_tag="WGQ71_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ0707137.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVTAAAKLGKVDLLVAGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQANAEKLVLTFRATAFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            16369..16980
                     /locus_tag="WGQ71_001123"
     CDS             16369..16980
                     /locus_tag="WGQ71_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ0707138.1"
                     /translation="MITTYKTITSLTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILERDLQLDCRLTVSLSLVNYEACVAAWKNTRQIEVNTEKPFE"
     gene            complement(17032..17349)
                     /locus_tag="WGQ71_001124"
     CDS             complement(17032..17349)
                     /locus_tag="WGQ71_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ0707139.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKYVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            17507..18778
                     /gene="purD"
                     /locus_tag="WGQ71_001125"
     CDS             17507..18778
                     /gene="purD"
                     /locus_tag="WGQ71_001125"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ0707140.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFSRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGRLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
BASE COUNT         4596 a         4336 c         5321 g         4745 t
ORIGIN      
        1 tcaattgttt ctgtatcgca aacactgatt gaaaaggact gattatggca tttttaaaac
       61 tgaccgaaca aaacgtgcag ggcaaaaccg tcctcatccg cgccgatatg aacgtgccgt
      121 tcaaagacgg caaaatcagc gacgacaccc gtatccgcgc ttcgctcgcg tccgtcaaat
      181 actgcttgga caacggcgcg tccgttatcg tgatgaccca cttgggccgc ccgactgaag
      241 gcgagttcca ccccgaagac gacgttgcgc ccgttgccgc gcatttgggc ggtctgttgg
      301 gcaaagacgt gaaagtattg aacgactggc gcgaaaacaa accggctttg aacgcgggcg
      361 atgtcgtgat gctgcaaaac gtgcgtatca acaaaggcga gaagaaaaac gatttggaac
      421 tgggcaaagc ctacgcttct ttgtgcgacg tgttcgtcaa tgacgcgttc ggcaccgccc
      481 accgcgccca agcctcgacc gaagccgtcg cccaagccgc gcccgttgcc tgcgccggcg
      541 tattgatggc gggcgaactc gacgctttgg gcaaagcctt gaaacagcct gcacgcccga
      601 tggttgccat cgttgccggc agcaaagtgt ccaccaagct gaccatcctc gaatcgctgg
      661 cggacaaagt cgaccaactc atcgtcggcg gcggtatcgc caacaccttc ctgttggcgg
      721 aaggtaaagc catcggcaaa tctttggcgg aacacgattt ggtggaagaa tccaaaaaaa
      781 ttatggcaaa aatggcggcc aaaggcggtt ctgtgccgct gccgaccgat gtcgtcgttg
      841 ccaaagcctt tgccgccgat gccgaagcgg tggtaaaaga cattgccgac gttgccgaag
      901 acgagatgat tttggacatc ggcccgaaat ctgccgccgc gcttgccgat ttgcttaaag
      961 ccgccgacac ggtcgtttgg aacggaccgg tcggcgtgtt cgagtttgac cagttcgcag
     1021 gcggtacgaa agcccttacc gaagccattg cccaaagcaa agcgttctcc attgctggcg
     1081 gcggcgacac gctggcggcg attgccaaat tcggcgttac cgaccaaatc ggctacatct
     1141 ccaccggagg cggcgcgttc cttgagttct tggaaggtaa agagttgcct gccgtagccg
     1201 ctttggaaaa acgcggcgag taaacggttt gatataaaaa aatgccgtct gaaatgttgt
     1261 gctgtttcag acggcatttt tgtgtggcgg ttattgtgct ttggcattcc attctgccgg
     1321 tgtggcggct accgaaatgg aaagtgcgtg cagttcgttg ctttccagtt gggctttaag
     1381 cccgtcttta atcaggcggt ggcgcgcgag gcgtgccttg ccttcaaatt ctgatgaaac
     1441 gatgacggcg aaaaaatgat gtccgtcgcc ttctacttcg atgtgttcgc attttgccac
     1501 gccttcaatc atggctttta cttgttccga ggtcagcatg gtaggttcct tgttggaaaa
     1561 atgcgccatt atactggcaa attgaaatgc cgtctgaaaa cgggttcgga cggcattttt
     1621 gacggtgttt tattttttgg ctttgaagcc gagcaggtgc tgggtggtgg caagccacgc
     1681 gccgaatatc cccagtgcga tgacgaagcc gaacaccagt gcgatttctc ccgggtagaa
     1741 gaaacgccag ccgatattaa gtccgtaggg tttgaagatg gcatcgacca aggggcgcac
     1801 ggcagagagc agccaaccgc aaagccccaa gctgacggcg gcggaaagga tgctctgcca
     1861 catggcttga taaaggaatg ggcggcggat aaacgacgcg ggcgcgccca agagtttggt
     1921 gatttcgatt tcttctttgc ggctgaggat ttgcagtcgg atggtgttgt gcgcgacaag
     1981 gacgaacgcc atccccagcg tcagggaaag aaaccataag attttacgga tgaattcgtt
     2041 gatttggtac agtgtctgta cccattccgt atccatgttg gcggattcga ccataggcag
     2101 tttggtaatg tcccggtaga ttgcctgcat ttgggcgggc gtggttgccg ggtcgggggt
     2161 aacgataaag acatccggca gggggttacc gtcaagcatg gaaatcagat tttggtcgag
     2221 attggactgt aattcttcca aaccgtcttc tttgctgata aagcggatgt tgtcgagccg
     2281 tttgtcgcgc accagcaggc tgcggacggt atcgctgtcg ctttgtgcgg cggcggtttc
     2341 catatagacg gtgatttgcg gcgactcatt gagtttgccc aacacgcttt gcccgctttg
     2401 gatgcccaag tacataaaca gcggcagggt catggcgacg gcgagcatta tgagggtaag
     2461 cagtgtgccg aaaggttggc gcagaagctg tttgagcgcg gtgcgcgcgg attcgacgtg
     2521 cagcgagagg tagtggatga tgctcatgcg agtcgtcctt tcgagaggcg caggatgcgg
     2581 tgtccgtagt ccgccatcag ggtttcgtca tgtgcggcga cgatgacggt tgttcccgct
     2641 tcgtggaagg ttttgaacaa ttccataata tcgagcgcgt aggcgcggtc gaggttggcg
     2701 gagggttcgt cggcaatcag caggctgggc tggtgaacga cggcgcgggc gatgcacagg
     2761 cgttgttgtt caccgccgga gagggttacg ggatcgtcca attctcgtcc tttcaggccg
     2821 actttttcga tggcgatgcg ggcacgctct tcggctttgc gcggcggata gccgataatc
     2881 cgaagcggca ggatgacgtt ttgcaggacg ttgcggtcgt agaggatttt gtggtcttgg
     2941 aacacgatgc cgatatgttg gcgcataaag ccgatttggt tgtcggacaa tgtgccgaga
     3001 tcctgcccgt taaacaggat tttgcccctg ctcggcttgg tgatgcccga aatcagcttg
     3061 aggacggtgg atttgcccga accggagtgt cccgcgataa atatcatttc gcctttgttg
     3121 atttggaagc tgacgttttt cagggcttca aaaccgccgg gataggtttt ggaaacttgt
     3181 tcgaaacgga tcataggagg tcctgtaagg aagggcggca tatcgtttcg ggcggttgcg
     3241 tccgcaggga tatgccgtct gaacaatagg taagacagtg ccgattataa agattccgcc
     3301 tgtttttaga tagtggataa gtttggcgga taccgaaggt cgggcgggaa aaatgccgtc
     3361 tgaagcggct tcagacggca tccggcgttt aacggcattt tgaatgggcg agtttgacgg
     3421 cttcggtcag gcttttttcg ttcacttccc cggtaatggt ttgtttgtac ccgcatttcg
     3481 gggcttcgac gacggtaaag ggcagtacgc cgacattgtt tccgtaggat ttcataaagt
     3541 ttcggctgtt cgcctcggtg taacgccaaa tcgggtagga aacaggagtt tgtttgagga
     3601 agttgccgat attgtcggat gtgtcgagcg cgatgccgac catatcgacg ctgccttttt
     3661 tctgcgcttt gtaccatttg gacatggcgg gcatctcttt tcggcacggg ccgcaccagg
     3721 ttgcccaaag gttgacgatg cgtacggggg ctttgagtga ttgcaggctt tgtggggtgt
     3781 tgtctttcca tccggccagt tcgtctgccg aggcggtatg tgcggcaaat gtggcggcaa
     3841 gggtgattgc ggcaagattc aagtatttca tcgttttgtc ctttgtatgt cgattcgggt
     3901 attgtaaacc gtttggtacg gttgtaggcg ggatattgag taaaaccctg tgtaagccga
     3961 taataagggg attgtcgaga agcaaggaaa tgtcatgcct gaaatcaaaa ttttccacaa
     4021 tccgcgttgc agcaaatcgc gtgccgcctt gtccctattg gaagaacgcg gcattgctgc
     4081 cgaagtggtc aaatatttgg atacgccgcc cgacttgtcc gaattgaagg atattttcaa
     4141 caaattgggc ttggcatcgg cgcgcgggat gatgcgcgtg aaagatgatt tgtataagga
     4201 attgggtttg gacaacccca atttggacaa tgacgcgctg ctgcgtgcca tcgccgatca
     4261 tcccgccctg ttggagcgtc cgattgtttt ggcaaacggt aaggcggctg tcggcagacc
     4321 gctggaaaat atcgaagcgg tattgtgaac aaacggctgt tttgcagccg caacggcttg
     4381 cgctattacc tgttgggcgg tttctgtttg agtgtttttc cgcttttgct ggtgtttgcc
     4441 tcatccgttt gggcggttta tcggacgggc gggcaggttt tgccgccgta tgtccgggcg
     4501 gatgccgcgc tggtgttggg cgccgccgcg tgggacaaac gcccttcccc ggttttccgc
     4561 gaacgcatca accacgccat cgcgctttat cagagccgca gggtaggcaa aatcgtgttt
     4621 acgggaggac gaaccaaaaa aggctatatg accgaagccg aggtcgggcg gcggtacgcg
     4681 ctcaaacagg gggttccggc gcgcaacatc ctgtttgaaa acacttcgcg caacacttat
     4741 gaaaacctga acaacatccg tccggttttg cgtgccaacg gtattgccag cgtggtgatt
     4801 gtcagcgatc cctaccattt ggcgcgggcg gcggaaatgg cggaggattt gggtgtccgc
     4861 gtttatatgt ctgccacccc gaccacgcgg tttgatgcgg gaaataagaa aaaaatcttt
     4921 atgctgcaag agggatatgc gttatccctc taccgtttgg aaaaatgggg cagccgcttt
     4981 ttgggatggc tgtctgattg aagcggtaga aaatgccgtc tgaaacttat ccgcaggcag
     5041 ggttcggacg gcatttttga tgcccaagca cggtaaaacc agtaaaataa acgctttcaa
     5101 agatttcgga gccatcatga ccgtcaaaac ccgtttcgcc cccagcccca ccggctacct
     5161 gcacattggc ggcgttcgca ccgccttgtt ttcgtgggcg tttgcccgcc atcataaagg
     5221 cgaattttta ttgcgcatcg aagacaccga cttggcgcgt tctaccgccg aatccgtcaa
     5281 catcatcctc gacggcatga aatgggtcgg cctgaactac gacaacgccg acaatgtcgt
     5341 gtaccaaacc cgccgtttcg accgctacaa agaagtcatt gccgaacttt tagaaaaagg
     5401 ccatgcctac tactgctatt gcagcaaaga agagctggaa gcgatgcgcg agaaagccga
     5461 aaaagaaggc acggcgactt acgaccgccg ctggcggccg gaagcgggca aaaccctgcc
     5521 cgaaattccc gccggcgtgc aacccgtcgt ccgcttcaaa acgcctttgg acggtgtaac
     5581 ccggtgggcg gacttggtca aaggcgaaat ttccatcccc aatgaagccc tcgacgacct
     5641 gattatcgcc cgatccgacg gctcgccgac ctacaacttc tgcgtcgtgg tggacgacta
     5701 cgacatgggc gttacccacg ttatccgggg cgacgaccat gtgaacaaca ccccgaaaca
     5761 aatcaacata ttaaaagcca tcggcgctac cctgcctgaa tacggccact tgcccatgat
     5821 tcttaacgaa caaggcaaaa aaatctccaa acgcagcggc gataccgttg ccattaccga
     5881 tttcggcgca atgggcatcc tgcccgaagc catgctcaac tatctggcac gcttgggctg
     5941 ggcgcacggc gacgacgagt tctttacgat ggaacaattc atcgaatggt ttgatttgaa
     6001 agacgtttcc ccgtccccaa gccgtatgga tttgaaaaag ctctattgga tcaacggcga
     6061 acacatcaaa atcacgccca acggcaaact cgccgaactc gtcaaaccgc gtctcgctct
     6121 gcgcgatatc catgaaactg aaaaacctgc attggaagac gtgttggcac tggtcaaaga
     6181 ccgcgcccaa gacctgaaca cacttgccga cgagtgcctc tacttctacg tcaaacaaac
     6241 gcctactgaa gcggacgtgc agaaacactg ggacgatgaa gccgccgccc gtatgctgcg
     6301 ctttgccgaa cgcctcgaag ggctggaaga ctggaatgcc gaagccatcc acgacctctt
     6361 caaacccttc tgcgacgaag aaggcattaa aatgggcaaa ctcggcatgc ccctgcgcct
     6421 tgccgtctgc ggtacggcga aaacccccag cgtcgatgct gtgttggcat taatcggcaa
     6481 agaagaagtg ttgaaacgca tccgcgctta agttatccac aggtgcaggc gatgccgtct
     6541 gatgtcggaa tacggcttca gacggcatcc aaacggaagt tgaacatgaa aaaaatattg
     6601 tattttctga tggttgtttt ttctacaagc gtatgggccg gggatgcgga agacaatctg
     6661 ctcagcatcc aatccggcta tcgtgcctta ctgcaaaagc aaaacaatct ggacggaaaa
     6721 atcatcggga tgcagtcgga tttggaagat gcgcgcagac ggttgcaggc ggctcaggca
     6781 gacatcgccc gtttggaagc ggaaattcct gcagcaatgg cgcaaaaagc ccggcaggct
     6841 gaagagctga ggcaaatcgg agtgcgtttg gaccatgctt ggaatgcggt ttacggcgca
     6901 gggggaacga aggcgtcggg gaattgaccg atgaattccc tctttgtgga caatactgtt
     6961 ttcattacac ggctgaaagc cgggcatatc ggcaggttgg ttcaggcgtt gtttgaggag
     7021 tggcacggat ttgaaccgtg gtcttctgtg gataagattc gtgcctatta cggcagatgt
     7081 ttgaaagatg acgaactgcc gctggctttt gcggctgtgg atgatttcgg aaccctgttg
     7141 ggttcggctg cggtcaagcg gcatgatatg aaaagtttcc cacagtatga atattggttg
     7201 ggggatgtct ttgttttacc tgaatatcgc ggaaaaggca ttggcaggag gctggtcgcc
     7261 cattgcatag gcgcagcgcg ttcgctgggg ataaagttct tgtatcttta tacgcctgat
     7321 gtgcaaatat tttatgaatc attcggctgg gcggttgtcg ggcgacattt ccataacggt
     7381 gaatgggtta cggttatgcg tttggatatg gataaggttt aagccgaatg gacaaatgcc
     7441 gtctgagctg ttcggacggc attttttata gtggattaaa tttaaatcag gacaaggcga
     7501 cgaagccgca gacagtacaa atagtacggc aaggcgaggc aacgccgtac tggtttaaat
     7561 ttaatccact atatgtttgg aaatttcctg tcagataatg tttatccaca gatttttcca
     7621 tatggtttga tgctgttccg acaacggaaa ttaagatacg gatttgcaca aatctttcag
     7681 acggtacagt tctgacaatg cttcgcgcgg agtcagtctg tcgggatcga gattttccaa
     7741 ggctgttgcc agtatacctt caaaatgttt ttcctctgct ttgtccacaa agttgcccac
     7801 attcagttca tctccttttt cagacggcat ggtactgaaa atatccagtt ggggacggtt
     7861 cgcggcggct tggttttcca gttcgttcaa atgcttttgg gcggatttca atgcgcgtac
     7921 aggcagtccg gcgagtttgg cgacggcgat gccgtagctt ttgccggcgg gacccggttg
     7981 gatttggtgc aggaacacga tgtcccgtcc ctgttcgagc gcggaaaggt gcatattgag
     8041 tgcggcggcg tgggcttcgg gcaggtaggt cagctcgaaa tagtgggtgg caaacaggct
     8101 gaaggatttg tttttttgca gcaggtgttc ggcaacggcg tgcgcgaggg cgaggccgtc
     8161 gaaagtggaa gtaccacgtc cgacttcgtc cattaaaaca aggctttgtt cggtggcgtg
     8221 atgcaggatg taggcggttt cgctcatttc gaccatgaaa gtggagcggt tggaggcgag
     8281 gtcgtccgat gcgccgatgc gggtgaagat ttgatcgatg ggcccgattg tggcggcatc
     8341 ggcaggcaca aaacagccgg tgtgtgccaa taaaacaatc agcgcgactt ggcgcatgta
     8401 ggtggatttg ccgcccatat tggggccggt gagcagcatg aggcggtgtt tgtggtcaag
     8461 gtcggtgtgg ttggcggtga agtggcgtac ctgctgttcg acaacgggat ggcggccgtt
     8521 ttcgatgtgg atagccggat agtcggcaaa cttggggcgg acgaagttcc gctcttttgc
     8581 caaggctgaa aatgtggaca acacgtccag cgcggcggcg gctttggcgg ctttttgaag
     8641 ctgcggcaat gccgtctgaa ggtttttcaa tacgccgtca aagagttgtt tttctaaggc
     8701 gagggcttgc tcttgagcag tcagcacttt gtcttcaaag gctttcaact ccggcgtgat
     8761 gaagcgttcg gcgtttttga gggtttgccg gcgttggtag tcggcaggtg cttgttcggc
     8821 ttgggttttg gataattcaa tgtaaaagcc gtgaacgcgg ttgaactcga ctttgagtgt
     8881 ggacaaaccg gtacgttcgc gttccttggc ttccaaatcc agcaaaaatt cgtcgccatg
     8941 gttttgaatg cggcgcaatt cgtccagttc gggatgaaaa ccgtggttga tgacattgcc
     9001 gtctttcagc cagacggcag gttcgggcat cacggcgact ttgagggttt cggcaactgg
     9061 cagggtttcg gggaaaacgg ctttgagggt ttccaacagg ctgctgccgt ttgcggacaa
     9121 gtcgatttcg gacagggcaa acaggctgtc gcgcagggcg gcgaggtcgc gcgggcgggc
     9181 gttgccgacg gcgatgcggg cagcaatgcg ttcaatgtcc gcaatgtttt tcagacggca
     9241 ttggaggggt ttgtattggc tttccagcgc ggcaacggct tcttggcgcg ctcggatgtg
     9301 ggcgcggttg cgtaaagggt ggtgcagcca gagagccaag aggcggctgc ccatatgggt
     9361 agcgcaaagg tcgagcgtgg acatcagggt cggcgatttt ttgccggaga gggtttgcgt
     9421 gatttcgaga ttgcggcgcg tggcggcatc cataccgata tattggctgt cggtttcgag
     9481 cgacaggccg tccaaatgtt gcggcatcag gttttgcgtc aggcggatat agttcaacag
     9541 tgcacctgcc gcgccaatcg caacggcgtg ttctttgccg tccaaaccga agccgcgcaa
     9601 atcctggcag ccgaaatatt ccgtcagcag tttttccccc gcgtcggcgg caaactgcca
     9661 cgcgttcagg cgcgtaacac ccgatgccgt ctgaagttgc ggtgcgtttt tactgtccgg
     9721 caacagaatt tccgccgcct gcaggcgcgc cagttcgtcg tccaatttat ccacagttgt
     9781 cagcttggtt ttgaattcgc cgctttgcag cgatgcccac gccaaaccga tgtatttttt
     9841 gtcgggggac acggcaacga tacggttggt ttccttgtct tccagcaatg cggaatcggt
     9901 cagcgtgccg ggcgttacga tgcgcacgac tttgcgctcc acaggccctt tgcccgcgcc
     9961 gacttcgccg acctgttcgc aaatcgccac gcttttgccc aacttgacca ggcgcgccag
    10021 atattgttcg gcggcgtgaa acggcacgcc tgccattttg accggctcgc catccacctg
    10081 tccgcgcgtg gtcagggtaa tatccaaaag ttttgccgct tctaccgcat cgtcgaaaaa
    10141 catctcgtaa aaatcgccca tacggtaaaa caccagtttg tcggtatgtt gcgctttgat
    10201 gccgaggtat tgctgcatca ttggggaaac ggcggatttg gacatggggc tgccttatca
    10261 ttaggaaaac ggtattgtag caaaaggttt tgtcttcgac ggaatgagac tgaccgtaag
    10321 cctgtcaata aggttgtgga gacaactagg aaaacagcaa agttatccac agaaatatta
    10381 ttaagtcaaa aaacatccac gcttatctgt cttatacagc aagtcttaaa ctcaaactta
    10441 tttgacaaaa agataatgaa aatattggat atttgctact tacgcacaag caggttggca
    10501 atcatcaaaa tcatcattaa aatttaaatt caaatattga tggtaaaggt tttggaaatc
    10561 tgtgcaaaca gcaaaattca tctatatcaa ataagcgtca agtagttaga tgtaatagtg
    10621 gaatgtaact ttatttcaga atacaatgca ggcatcggac tttatttcac aacctggaga
    10681 caaaatatga gcattaaagt agcgattaac ggtttcggcc gcatcggccg cctcgcattg
    10741 cgtcaaattg aaaaagccca cgacatcgaa gtcgtcgccg tcaacgacct gacccctgcc
    10801 gaaatgctgc tgcacctttt caaatacgac agcacgcaag gccgtttcca aggtactgcc
    10861 gaattaaaag acgatgccat cgtggtaaac ggcaaagaaa tcaaagtttt tgccaatccg
    10921 aatcccgaag aattgccttg gggcgagttg ggtgtggatg tcgtcctcga atgtaccggc
    10981 ttctttacca ataaaaccaa agccgaagca cacatcaggg ccggcgcgcg caaagtcgtg
    11041 atttccgcac ccggcggcaa tgatgtgaaa accgtcgtat atggcgtaaa ccaagacatt
    11101 ttggacggca gcgaaaccgt tatctccgct gcttcctgca ccaccaactg ccttgccccg
    11161 atggcggcag tcctgcaaaa ggaattcggc gtagtcgaag gcctgatgac caccatccac
    11221 gcctacaccg gcgaccaaaa cacccttgac gcgccgcacc gcaaaggcga tttgcgccgc
    11281 gcccgcgccg ccgcgctcaa catcgtgccc aacagcaccg gtgccgccaa ggccatcggt
    11341 ctggttatcc ctgaattgaa cggcaaactc gacggctccg cccaacgcgt ccctgttgcc
    11401 accggctcgc tgaccgaatt ggtttccgtc ctcgaacgcc ctgtaaccaa agaagaaatc
    11461 aacgccgcga tgaaagccgc cgccagcgaa tcttacggct acaacgaaga tcaaattgtt
    11521 tcttccgacg ttgtcggcat cgaatacggt tcgctcttcg atgcgaccca aacccgcgtg
    11581 atgaccgtgg gcggcaaaca attggtgaaa actgttgcct ggtacgacaa cgaaatgtcc
    11641 tacacctgcc aactcgtccg cactttggaa tactttgcag gcaaaatcta aggcttgaac
    11701 aaacctgtgg ataagtaaat gccgtctgaa cacattgcgt tcagacggca ttgtttatct
    11761 gtatagtgga ttaaatttaa accagtacgg cgttacctcg ccttgccgta ctatttgtac
    11821 tgtctgcggc ttcgttgcct tgtcctgatt taaatttaat ccgctataac ttgatattgc
    11881 ggcttatggc gaacaaacaa cagccgtttg ttcaactgct atgcggcaaa accggatttt
    11941 tgcccgattt aaagcggggt tggggaaaga tcaataacgg ttgcacggtt ttcctcgatt
    12001 tgaaatctga cttcgtaatc cgcaatattc atcacataaa tccgttcggg aatatcctga
    12061 taggctgggc gcggatcttg ggcaatgctt tggctgataa ggtttttggt gtttgcagat
    12121 aaattttccg cgccgatgtt ttcctgccaa acgacttcca actctacggg tttgccgctg
    12181 acgaaaccag atgcggcatc gggtttggat tcgacaaagg ggatataagg tttgatgtcc
    12241 acaatcggtg tgccgtccag caggtctgca ccgctgcaat agaggcggac gggtttgccg
    12301 gtttcgatgc gttcgagttt caggagcgag agtccgagat ggttggggcg gtgggggctg
    12361 cgcgtggcga acacgcccat tttttgtttg ccgccgagcc gtggtgggcg caccatttgc
    12421 gcccagcctt catccaatac gccgtgaaaa ataaaactta tccacacata gtcgaagtgt
    12481 tccagtccgc gtacgctgtc tgcggtaaat tcgggattca gctcgatgca tacttctgcg
    12541 gcagagacca aaccgggctg gcgggcgatg ccgaatttct gtttgtaggg cgatcgggcg
    12601 gtggcgatag ggacgatggt gtaagtcatg aggattgtgg ataagttttt cggaaaaacc
    12661 ttatcttatc acagcctgtt ttaccgcttt tgggcagtaa tttgttataa tacagggcat
    12721 tttaattgcg ttgaaatgat gagatgattg tttccattga tgttgatgca caaaaaactt
    12781 ttacgccttt gtgtcctaaa gagctgcctg taaacgaggg gcatttgatt gtcgaagaat
    12841 tgaatgccca agccgctttg gcggatttgc gcgtgatgac gaaagatgcg catcatgcgg
    12901 cagcaaaatg gcttgtggat aaccctgttg atatgttgaa gccgacaggt ttgcccgatg
    12961 cggatttgac ttgggtggct catgcgatgg ttggtacgcg cggctatgaa ttgttggacg
    13021 ggctgccttg tgccaaagaa tacgattatt gcgtttggaa aggtgttgat cctgaattgc
    13081 atccttacgg cgcgtgtttt cacgatattg aggaaaaact aagcacaggg ctgattgaat
    13141 ggctgcgttg tcaaaatacg gatacggtta ttatcggtgg gttagctacg gattattgtg
    13201 ttaaaacaac ggttttgcag ttactcaaag gcggtcgttg gcaggttgtc gtcaatgaag
    13261 cggcttgtcg gggtattgcg ccggaaacca tcgaaacagc atggcaggaa atgcattctg
    13321 ccggggcaat catcttaaaa aacgctgatg aaataaataa atatattaat aatcaataag
    13381 ttaaaattgt ttcacgtgaa acccccttcc taaatatgaa aattttgctt gtccgcttgt
    13441 ctagtatggg cgatttaatt cacactttgc ccgcaatcga agatttggcg cgacaatgtc
    13501 ctgatgtaga actgcattgg ctatgtgagg ctggatttgc agatattgcg cgcctgcatc
    13561 cgtttgtaaa aaaaatccat gtgatgaaat ggcggcaatg gcgcaaacat ctctttcggg
    13621 ctgaaacttg gcgggaaatg ggtcatctga aacagacttt gcggcaggaa gtatttgatt
    13681 tcgtattgga cagtcaaggt ctgattaaaa gcgcgtgttt cgctaaaatg gcaaaatccc
    13741 cgatttgtgg tttggataaa aacagtgcgc gcgagggatg ggctgctttg gcgtatgtaa
    13801 aaacatacgc tgtaccgaag ggaaaaaatg ccgtttggcg caaccgtgaa ctgtttgccc
    13861 aagtatttgg gtatgtaatg ccggaaacgc aggtatttgg cttgactgtt cctgaagcag
    13921 gccgtctgaa aaatttagcg cagccgtatt atgcggcttt gcatgcgacc agtcgggaca
    13981 gtaagttatg gcctgtggaa aactggcggt cattgctgca aaagttgaat gaagaacagc
    14041 aatgcaatat ttacctgcct tggggaaatg aagatgaaaa aacgcgtgcc aaacaaattg
    14101 cagacggact gccgtttgct attgtgtgcg acaaaataaa tttattgcag gcagcgtatc
    14161 tgctgaaata cgcggtcgga attgtcggcg tggataccgg tttgctgcat ttggcaaatg
    14221 ccttggaaaa acctgtggtc ggcatttata ccgataccga tccgattaaa acaggcgttc
    14281 aggtttcgcc aattgcaaaa aatttgggca atatcgggca gattccgacc gcagatttgg
    14341 tttatcaagc gctgatggat tgtgttgcag cagataaagg ctaaagggtc gtctaaaact
    14401 gatatggcgg catgatattt tccaatttat tagatacagg aatgtatatc cgctagtctg
    14461 agtaaatgct ttaaaaatgg tataatcggc aagcattatc cgaaagattt ttgaatgaat
    14521 tcgtttttaa ggaaaacgga tttggtttta tagggaagaa ggtttggttt cgctggatgg
    14581 tgaaagcagc gttcttttta ggagggttca atgaaagcac tggtcgcagt aaagcgcgta
    14641 gtggactaca acgtcaaagt tcgtgtaaaa gccgatggtt cggatgtgga tatcggcaat
    14701 gtcaaaatgt cgatgaatcc gtttgacgaa atcgctgtgg aagaagccgt ccgtttgaaa
    14761 gaagccggaa aagtaagcga gatcgtagcg gtttctttgg gcgaaaagaa atgcgaagaa
    14821 accttgcgta cagctttggc gatgggtgcc gaccgtgcaa ttcatgttga aaccgatgcg
    14881 aaattggagc cgctggcagt tgccaagttg ctgaaagccg ttgcggacaa agaaaatccg
    14941 caaattttct ttttgggtaa acaagcgatt gatgacgatg ctaatcaagt ggcgcaaatg
    15001 ctggcagctt tgttgaatgc ggcgcaaggt acgtttgctt caaaagtaca aattgaaggt
    15061 gatgaagtac agattgtgcg cgaaattgat ggcggcgaag aaaccatagc attgaaattg
    15121 cccgctgtta tcagtgcaga tttgcgtttg aacgagccgc gcttcgtcaa actccccaat
    15181 attatggcgg cgaagaaaaa acctttggaa aaactggctc ctgccgattt ggttgctgac
    15241 atttcacctc gtctgaaaac ggtgaaattc gccgagccta aagcgcgtca ggcaggcgta
    15301 aaagtagcaa gcgttgccga attagttgaa aaattgaaaa acgaagccaa agtgatttga
    15361 ggagactgaa atgagcgtat tgattattgc cgaacacgac aacaaacagt tgaatcctgc
    15421 cactttgcat gctgttaccg ctgccgcaaa actaggcaaa gtcgatttat tggttgccgg
    15481 aaacggcgca tcgtccgtcg tggagttcgc gaagcaagta gcgggagtgg aaaaagtttt
    15541 ggttgcagat gctgcccatt atgctgaagg tttggctgaa gagttggctc cgctggttgt
    15601 caaattggcg gcggattacc gctatgttgc agcaacggca accacattcg gtaaaaacct
    15661 tttgccccgc gtagccgcct tattagacgt accgcaaatt tctgatttga ccgagattgt
    15721 agataacacg acttttgtac gtccgattta tgcaggtaat gcatttgaaa ccgtgcaagc
    15781 caatgcggaa aaattggtgc tgaccttccg tgcgacggct tttgacgcag tggcggcgca
    15841 aggtggtaat gctgaggtaa ttaatgttga agcaacccct gcccaaaacc tgagtcgttt
    15901 tgttaaccgt cagctttccc attccgatcg tcctgaactg actcaggcaa aagtgattgt
    15961 ttccggtggt cgtgcgttgg gtagtgcgga aaaattcaat gaagtgctga caccgttggc
    16021 ggatgtttta ggtgcggcaa tcggtgcatc ccgtgcagca gttgatgccg aatatgcgcc
    16081 aaacgatgct caagtcggac aaaccggtaa agtggttgcg ccgcaactct attttgcaat
    16141 cggtatttca ggtgcgattc aacacgttgc cggtatgcaa gacagtaagg tgattgttgc
    16201 aatcaataaa gatgctgatg cgccgatttt caatgtagcc gattacggat tggttggcga
    16261 tttatttgaa atcgttccac aattaacaga agcattgaaa aattgatgtt tcacgtgaaa
    16321 cgttcagacg accttgttat taatgaggtc gtctgaaaac atttcagcat gattacgact
    16381 tataaaacca ttacctcctt gacgcaggct gagtttaaag ataaaggcag ccgttttatt
    16441 gcatttgcct atccgattcg gacattggct gatgtgaaaa aatacctcga tccgctaaag
    16501 gaagagcatc ataaagcacg acactggtgc tatgcctatc gtttgggtgt ggatggtgtg
    16561 caatttcgtg ccaacgatga tggagagcca tcaggaagtg ctggacgacc aattttggga
    16621 cagattgatt cggtgggtat taccgacgtt ttggtcgtgg tcgtccgcta tttcggtggt
    16681 actttgttag gtgttccagg tctgatacat gcgtacaaag aggcgacggc tcaagcgttg
    16741 gcagttgcag aagtagttga aaagaatatt gaaaaaactg tttggctgaa atgtgaatat
    16801 ccgtttttga atgaagcgat acgcattgcc aaacaatatc aggcggatat attggagcgg
    16861 gatttgcagt tggattgccg attgacggtc agtttgtcgt tggtaaatta tgaggcctgt
    16921 gttgcggctt ggaagaatac acggcagatt gaagtaaata cggaaaagcc ttttgaatga
    16981 aaggtcgtct gaaatgcttt gtgatggtat tttagacgac ctcttcgctg actaatcttc
    17041 ttgtgtatat ttgtctaagg catcgacgct ggagcgcaaa tataaccaag ccaattgatg
    17101 gaattcgccc agcgcgttcc atagcgcatc ttcactcata atgctgtatt cgccttccgt
    17161 acgcaaatcc agttctaccc cgtcttcaat agcctgatga cacgcctgat actcgtgggt
    17221 atagaaattg tccatgtcgc gaattaaagt gacgacatat ttccaacggc ggatgtcttc
    17281 ttccagttgc ggtagtaaat gacaccattc gcggtatttg ctttggattt catcaatacg
    17341 tttttgcatg gttgcttgtc cttttttaat ttatggataa gttgaatagg ttgcagagtg
    17401 gaatgaagcg gtcttttgtt tgattctact aaaatttctt cagatgggat aaaagtccct
    17461 ttgtatcgag ttttcgttaa aagtacatat ttgtcaagga gatggtatga aactgctggt
    17521 tatcggtaat ggcggtcgcg aacacgcgct ggcttggaaa ttggcgcagt cgcctaaagt
    17581 ggaaacagta tttgttgcgc ctggtaatgc cggtacagcg attgaaccca agttgcaaaa
    17641 catcgacttg actgcgcatc aggatttgat tgaattcagt cgtaaagaaa atattgtttt
    17701 taccgtcgtc ggccctgaag cgcctttggc ggcaggtgtt gtggatgatt tccgtgccgc
    17761 aggactaaaa atattcggtc cgacacaata tgcggcgcag ttggaaagtt ccaaagattt
    17821 cgccaaagca tttatggcga aatacaacat tccgaccgcg caatatcaaa cctttgaaaa
    17881 cgccgatgcc gcacatgatt acgtcaatca gaaaggtgcg cctatcgtta tcaaagccga
    17941 tggtttggcg gcaggtaaag gcgtgattgt ggcgatgact ttggatgaag cgcatgctgc
    18001 gattgatgat atgctgctgg acaacaaaat gggcaatgcc ggcgcgcgtg ttgtgattga
    18061 agatttcctg caaggcgaag aagcgagttt tatcgtcatg gttgatggca ataatgtact
    18121 gcctatggca accagccaag accacaagcg tcttttagac ggcgacaaag gtctgaatac
    18181 gggcggtatg ggtgcgtaca gccctgcgcc tgtggtaacg cctgttgtgt acgagcgcgc
    18241 gatgaatgaa attattttgc cgaccgtagc agggatgaaa gcggaggggc atgagttcac
    18301 cggtttcctg tacgcaggtt tgatgattga tcaaagcggt gcaccctata cgattgagtt
    18361 taactgccgt ttcggcgatc ctgaaaccca gccgattatg agccgactga acagcgactt
    18421 atcggatttg gttgaagcgg caatagacgg cagactcgat agcgtaaccg cggaatggag
    18481 cccgcaaact gccgtgggcg tggtactggc ggcgcaaaat taccccgaaa cgcccaaaaa
    18541 aggcgatgtt atttccggtc tggatgctgc caaccaagta ggcaaagttt tccatgcagg
    18601 tacaacggca aacgagaaag gtgacgtatt gaccaacggc ggacgcgtat tgtgtgttgt
    18661 gggattgggc gacaatgtgg cgcaagcgaa agctaaggca tatggcgcgt tagaaaaaat
    18721 cagtttcgac gggatgcaat accgtaaaga cattgccgat aaagcgatta accgttaact
    18781 agaaagagga tgatgccgtc tgaagccaag tatgactgat gtgcttcaga cggtatttta
    18841 tattatgaga cctttgcaaa attccccaaa atcccctaaa ttcccaccaa gacatttagg
    18901 agatttctca tgagcacctt cttccagcaa accgcccaag ccatgattgc caaacacatc
    18961 gaccgtttcc cgctattgaa gttggaccag gtgattga
//
LOCUS       DAWFIE010000035        18447 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWFIE010000035 DAWFIE010000000
VERSION     DAWFIE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18447
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.035"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(241..1344)
                     /locus_tag="WGQ71_001126"
     CDS             complement(241..1344)
                     /locus_tag="WGQ71_001126"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ0707141.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1579..3135)
                     /gene="dnaA"
                     /locus_tag="WGQ71_001127"
     CDS             complement(1579..3135)
                     /gene="dnaA"
                     /locus_tag="WGQ71_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ0707142.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     VSEGMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRQAAGSASRPESVA
                     VAKARTDVQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3427..3561
                     /gene="rpmH"
                     /locus_tag="WGQ71_001128"
     CDS             3427..3561
                     /gene="rpmH"
                     /locus_tag="WGQ71_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ0707143.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3564..3929
                     /locus_tag="WGQ71_001129"
     CDS             3564..3929
                     /locus_tag="WGQ71_001129"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ0707144.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRSTAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            3994..4215
                     /gene="yidD"
                     /locus_tag="WGQ71_001130"
     CDS             3994..4215
                     /gene="yidD"
                     /locus_tag="WGQ71_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ0707145.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            4387..6024
                     /gene="yidC"
                     /locus_tag="WGQ71_001131"
     CDS             4387..6024
                     /gene="yidC"
                     /locus_tag="WGQ71_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ0707146.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQTAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGECSIDI
                     KRRNDKLYSASVSVPLAAIPAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(6105..6818)
                     /locus_tag="WGQ71_001132"
     CDS             complement(6105..6818)
                     /locus_tag="WGQ71_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ0707147.1"
                     /translation="MMSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLLELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHSETRLCTATDLTLPTQEIISQTVVQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(6877..7467)
                     /gene="maf"
                     /locus_tag="WGQ71_001133"
     CDS             complement(6877..7467)
                     /gene="maf"
                     /locus_tag="WGQ71_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ0707148.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCALKSEDL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7615..8118
                     /locus_tag="WGQ71_001134"
     CDS             7615..8118
                     /locus_tag="WGQ71_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ0707149.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSS"
     gene            8152..8331
                     /gene="rpmF"
                     /locus_tag="WGQ71_001135"
     CDS             8152..8331
                     /gene="rpmF"
                     /locus_tag="WGQ71_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ0707150.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            8506..9033
                     /locus_tag="WGQ71_001136"
     CDS             8506..9033
                     /locus_tag="WGQ71_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ0707151.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            9121..10176
                     /gene="plsX"
                     /locus_tag="WGQ71_001137"
     CDS             9121..10176
                     /gene="plsX"
                     /locus_tag="WGQ71_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ0707152.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTAQIVGMDEAPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            10312..10563
                     /locus_tag="WGQ71_001138"
     CDS             10312..10563
                     /locus_tag="WGQ71_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0707153.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            10814..11005
                     /locus_tag="WGQ71_001139"
     CDS             10814..11005
                     /locus_tag="WGQ71_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707154.1"
                     /translation="MIQTILEDGDIEVKNLKTENYSGKAREILGDSYPIEHISKNIST
                     IQNIISEFMIQKQTPAPII"
     gene            11025..11228
                     /locus_tag="WGQ71_001140"
     CDS             11025..11228
                     /locus_tag="WGQ71_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707155.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLSVLGNHRFFAYNSRI"
     gene            11255..12217
                     /locus_tag="WGQ71_001141"
     CDS             11255..12217
                     /locus_tag="WGQ71_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ0707156.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDATGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            12264..12635
                     /locus_tag="WGQ71_001142"
     CDS             12264..12635
                     /locus_tag="WGQ71_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ0707157.1"
                     /translation="MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            12661..13587
                     /gene="fabD"
                     /locus_tag="WGQ71_001143"
     CDS             12661..13587
                     /gene="fabD"
                     /locus_tag="WGQ71_001143"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ0707158.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFAEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LNFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKAICAEAAQGEVVEAVNFN
                     SPGQIVIAGNAAAVERAMTAAKEAGAKRALPLPVSVPSHCSLMKPAAEKLAEALKSVV
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIEAH"
     gene            13725..15590
                     /gene="msbA"
                     /locus_tag="WGQ71_001144"
     CDS             13725..15590
                     /gene="msbA"
                     /locus_tag="WGQ71_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ0707159.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIIAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            15690..17255
                     /gene="guaA"
                     /locus_tag="WGQ71_001145"
     CDS             15690..17255
                     /gene="guaA"
                     /locus_tag="WGQ71_001145"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ0707160.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPILGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDDTPNTLDVWMSHGDKVSKLPTGFTVIGDTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVMY
                     DVSGKPPATIEWE"
     gene            17317..18063
                     /gene="fabG"
                     /locus_tag="WGQ71_001146"
     CDS             17317..18063
                     /gene="fabG"
                     /locus_tag="WGQ71_001146"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ0707161.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            complement(18325..>18447)
                     /locus_tag="WGQ71_001147"
     CDS             complement(18325..>18447)
                     /locus_tag="WGQ71_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0707162.1"
                     /translation="FRYARAAYFGLLKVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT         4634 a         5583 c         4393 g         3837 t
ORIGIN      
        1 atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac aaatagtacg
       61 aaaccgattc acttggtgct tcagcacctt agagaatcgt tctctttgag ctaaggcgag
      121 gcaacgccgt actggttttt gttaatccac tatacctttc cgacagccga acaaaacccc
      181 gaatccgtct gcacggttcg gggtatatct ccaatacggg catcgtgttc cggaaaaccg
      241 tcaaatccgc atcggcatca caatatattt gaaattcgga ttgttcggca cggtaaacag
      301 cgtcgagcgg ttggcatcgc cgaaggcaag ctgcatatcg tcggaatgga tgttgcgcaa
      361 cacgtccatc agatagccga tattgaaacc gacttcgagt tcgccgccct gataggcaat
      421 ctcgatttct tcgcgcgctt cttcctgctc gttgttgctg cacacgacgc tcaacaggcc
      481 ggattgcagg aacaggcgcg cgccacggaa tttttcgttg gcaagaatgg acacacgttc
      541 cagcgcgccc aaaagttcgg cacgggacaa aacgaaaatc ttgtcgttgt ccaaagggat
      601 gacgcggttg aaatcgggga atttgccgtc gataaccttg ctgacgatgg tcgtgccgtt
      661 gcattggaaa cgcacctgct tgtccagcag ctcgatttga atcggatcgt cggggttgtt
      721 caacagtttg aacagttcca gcaccgtttt gcgcggcaaa atcacttcgg cgcgcggcaa
      781 atccgcatca atcgcgcagg ctgcataggc aaggcggtgt ccgtcggtcg ccacaaggcg
      841 caactggctg ccctcaacct gcatcagcag accgttgaga taatagcgga tgtcctgcac
      901 cgccatgctg tactgcactt gcgacagcat ggttttgaaa cgctcctgct ccagcgagaa
      961 agtcgcgctg atgtcctcgc cgacattcat catcggaaaa tcggcggcag gcagggtctg
     1021 cagggcaaaa cgcgatttgc ccgccttcag cgtcagacgg ctgtcgtccc aatccagcga
     1081 caccagcgca ccggcaggca gtgcccgtaa aatgtcctga aatttcttgg cattggtggt
     1141 gatgcggaag tcgtccgcgc cgccctcggg acccgcagtg tcgatttgga tttccaaatc
     1201 ggttgccaag agtttggtct gaccgccttt tccctcaatc aggacgttgg acaggatggg
     1261 cagggtgtgg cggcgttcga caatgccggt aacggcttgc aacggcttga gcaggctgtc
     1321 gcgctcggct tgtaaaatca acatgttcgc tcctttaaat cggtttgtat agtggattaa
     1381 atttaaatca ggacaaggcg acgaagccgc agacggtaca aatagtacgg aaccgattca
     1441 cttggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgccgta
     1501 ctggtttaaa gttaatctgc tatatcttta cccttcggac ggcatgggca atatcatgtc
     1561 gtctgaaaac gttttccatc agttttgaat cagaatcagc agcttttcat aatcctgagc
     1621 caattccgga tcttcttcgc gcagtttcgc cactgccctg atgccgtgca taacggtcgt
     1681 atggtcgcgc ccaccaaacg aatcgccgat agacggcagg ctcaaagtag tcagttcttt
     1741 ggtcaggctc atcgccacct ggcgcggacg ggcaatgttg cgcgtgcgtt ttttgccgag
     1801 tacgtcgctg attttgatgc ggtaatattt cgccaccgca tcgatgatga tgtcggcggt
     1861 gatgactttg tgcttctcgg caataatgtc ctgcaaagcg gtacgcgcca aatcgatgtc
     1921 gatgacggga cggttcataa agcggctgct cgctccgaca cgattaaacg cgccttcaag
     1981 ctcgcgcacg ttggaacgga tcagattggc aatgaacagc gcggcttcgt cttcgatact
     2041 gatgcccgcc gcttccgcct ttttctgcaa aatggcgatg cgcatttcca attcgggcgg
     2101 ctcgagttcc aaagtcagtc cccatgaaaa acgggatttg aggcggtcgt ccatgccttc
     2161 gattttcgca ggcaacacat cgcaagtgag gatgagctgt tttttctcgt tgtggaaatg
     2221 gttgtacaga tagaaaaact cttccatcgt acggtctttg cctttgatga actggatgtc
     2281 gtcgataatc agcaggtcgt attgcttgta ttgctgcttg aacacgtcgt aagtgttgtt
     2341 gcgaaccgcc ttcataaagc tgcggatata gtcatccgaa tgcatatagc gcactttggc
     2401 atcgggacgg tttttcagca gctcgttgcc gaccgcctgc acaaggtggg ttttgcccaa
     2461 acccgtactg ccatagagga agaacgggtt gtaactctgc cccgggcttt ccgcaatcgc
     2521 ctgcgccgca gccgccgcaa ggcggttgcc cttaccttct accaacgtat caaacgtgta
     2581 atccggagac aggttggtct gctcgtaacg cgcctcttcc gcatcgcgct gcacgtccgt
     2641 ccgtgctttg gcaactgcca ccgattccgg ccgggaagca gacccggcag cctgacgcgg
     2701 ctcgtgcggc aggtttttca tacgttccgc caaaatatcc gccgccgttt tcgacgcagc
     2761 gggtttgaca ggctcttcag acggcagctc gtccaacaga acctcctgca cgggcattcc
     2821 ctccgacacc gcatgcaagg acggctcggc aggttcgaca gcaccttcaa ccgccgccat
     2881 ctcataacgc acgccttctc ccggtttgaa tacgaaggcg gaacggccgg cagccaactc
     2941 ttccctcacc gcttctattt ttccggcaaa ctggctcttg agcatattgc aggcaaactg
     3001 gttcttgccg tacaccaccc atacgccacc ctcctcacca acggtaaggg gcgcaatcca
     3061 ttgcgcaaac tgcccttgag gcaacatatc gtgaagacgg cggaggcaca gcggccaaaa
     3121 ctctgctaat gtcatggata ggctcgaatc ggtaaaaatg aaatcgaaaa caaagaaaat
     3181 ataatatttt caaaaagaaa acaaatctgt tgaacgcaca tcggttcaaa acgcgactgc
     3241 ccgattatac cgactcacga atattttatc cacaacccgt gcaaaaattt atccacagaa
     3301 aggcggcgga aatccgcagg caatcgggca atcttcctgc aaagtttcta tattgattga
     3361 caaaagcggc aaattggagt gtaattcacg gtttaattat ctacccattc tattttagga
     3421 aacatcatga aacgcactta tcaaccttcc gttaccaaac gcaaacgcac ccacggcttc
     3481 ctggtgcgct ccaaaacacg cggcggccgc gcagtattgg cggcacgccg tgccaaaggc
     3541 cgcaaacgcc tggcggtgta attttggact accgcttcgg aaggcagtac cgcttgttga
     3601 aaacggatga tttttcatcc gtttttgcat tcagaaaccg ccgcagccgc gacctgctgc
     3661 aagtttcgcg ttcaaacggc aacgggctgg accatccccg catcggtctg gtggtcggca
     3721 aaaaaaccgc caaacgcgcc aacgaacgaa attatatgaa gcgcgtcatc cgcgactggt
     3781 tcagattaaa taaaaaccgg ctgccgccgc aggatttcgt cgtgcgcgtc caccgtaaat
     3841 tcgacaggtc taccgcaaaa caggcaaggg cggaactggc acaactcatg ttcggcaacc
     3901 ccgcaaccgg atgcaggaaa caggcatgat cagaacggta ctctgcaggc aaggttcaga
     3961 cggcaacggg tttcccatac aaggaacatc ccgatgaact tcctattgtc caaactcctg
     4021 ctgggactga tacggttcta ccaatattgc atcagcccgc tgattccgcc gcgctgccgt
     4081 tatacgccga cctgttcgca atacgcggtc gaagcggtca aaaaatacgg cgcattcaaa
     4141 ggcggccggc tcgccatcaa gcgcattgca cgctgccacc ctttcggcgg acacggacac
     4201 gaccccgttc cctgacccga cgcaatattc aaattgcacg ctttcctttt atttcccatc
     4261 ggtttctata taatgccgtc tgaaccttcg ggctggcggc acgaccgtcg ggtatgaagc
     4321 ccgcccttat tccccgtcta tcggaacacg caacctgcgg catttccgac cattcaggaa
     4381 actcttatgg attttaaaag actcacggcg tttttcgcca tcgcactggt gattatgatc
     4441 ggatgggaaa agatgttccc cactccgaag cccgtccccg cgccccaaca gacggcacaa
     4501 caacaggccg taaccgcttc cgccgaagcc gcgctcgcgc ccgcaacgcc gattaccgta
     4561 acgaccgaca cggttcaagc cgtcattgat gaaaaaagcg gcgacctgcg ccggctgacc
     4621 ctgctcaaat acaaagcaac cggcgacgaa aataaaccgt tcatcctgtt tggcgacggc
     4681 aaagaataca cctacgtcgc ccaatccgaa cttttggacg cgcagggcaa caacattcta
     4741 aaaggcatcg gctttagcgc accgaaaaaa cagtacagct tggaaggcga caaagttgaa
     4801 gtccgcctga gcgcgcctga aacacgcggt ctgaaaatcg acaaagttta tactttcacc
     4861 aaaggcagct atctggtcaa cgtccgcttc gacatcgcca acggcagcgg tcaaaccgcc
     4921 aacctgagcg cggactaccg catcgtccgc gaccacagcg aacccgaggg tcaaggttac
     4981 tttacccact cttacgtcgg ccctgttgtt tatacccctg aaggcaactt ccaaaaagtc
     5041 agcttctccg acttggacga cgatgccaaa tccggcaaat ccgaggccga atacatccgc
     5101 aaaaccccga ccggctggct cggcatgatt gaacaccact tcatgtccac ctggatcctc
     5161 caacccaaag gcggacaaag cgtttgcgcc gctggcgaat gcagcatcga catcaaacgc
     5221 cgcaacgaca agctgtacag cgcaagcgtc agcgttcccc ttgccgccat cccggccggc
     5281 gcgaaagccg aaaccgccgt caacctgtat gccggcccgc aaaccacttc cgtcatcgca
     5341 aacatcgccg acaacctgca actggccaaa gactacggca aagtacactg gttcgcctcc
     5401 cccctctttt ggcttttgaa ccaactgcac aacatcatcg gcaactgggg ctgggcgatt
     5461 atcgttttaa ccatcatcgt caaagccgta ctgtatccat tgaccaacgc ctcttaccgc
     5521 tctatggcga aaatgcgtgc cgccgcaccc aaactgcaag ccatcaaaga gaaatacggc
     5581 gacgaccgta tggcgcaaca acaggcgatg atgcagcttt acacagacga gaaaatcaac
     5641 ccgctgggcg gctgcctgcc tatgctgttg caaatccccg tcttcatcgg attgtattgg
     5701 gcattgttcg cctccgtaga attgcgccag gcaccttggc tgggttggat taccgacctc
     5761 agccgcgccg acccctacta catcctgccc atcattatgg cggcaacgat gttcgcccaa
     5821 acttatctga acccgccgcc gaccgacccg atgcaggcga aaatgatgaa aatcatgccg
     5881 ttggttttct ccgtcatgtt cttcttcttc cctgccggtc tggtattgta ctgggtagtc
     5941 aacaacctcc tgaccatcgc ccagcaatgg cacatcaacc gcagcatcga aaaacaacgc
     6001 gcccaaggcg aagtcgtttc ctaaatgccg cagcatgaaa aatgccgtct gaaacctgtt
     6061 cagacggcat ttttattgtt caccccctat cggggcggaa atcttcaacc tgcatacatc
     6121 acaaaaatcg tcgggcgttt tttcagattg ggcatttctt ttctttttcg ccactgcaca
     6181 accgtctggc tgatgatttc ctgagtcggc aacgtcaaat ccgtagccgt acacaaacgc
     6241 gtttcagaat gcaggttttc caccgcatcc gccaacaaca catcgttgcg atacggcgtt
     6301 tcgataaaca gctgcgtttc attctgctgg cgcgaacgtt gttccaaagc cttcaaactc
     6361 tgaatccgct cgtttttttc agacggcaga taacccttaa acgcaaagtt ctgcccattc
     6421 gcacccgaag ccatcagcgc cagcaacaag ctggacggac cgaccaaagg ccgtacttca
     6481 aaaccgtgtt tatgcgccaa tgccaccaaa ttcgcacccg gatcggcaac agccgggcaa
     6541 cccgcctcgc tgacaatgcc catactgcgc ccttcttgca aaggtttcag caattccagc
     6601 aaagtcttca aatccgtgtg ttcgttcagc gtttgcaaat tcagttcgcg gataggcatt
     6661 gtcacgccca aatgtttcaa atgcgcgcgc gccgtttttt ccgcttccac gacaaaatcc
     6721 gtcagcccga caatcgcctg ttgttcatgc ggcaacaggc acggcgtgtc aggcgtaccc
     6781 aaaggcgtag gaatcaaata caaaacagga gacatcattc cctcattcat cggttaaaaa
     6841 tgccgtctga gcctttcaga cggcataaac gggcagttac agaacctcca cgccctcatt
     6901 tttcaagaaa tcaaccagac ggaaaaccgg caaaccgatt aaagcattcg gatcggtact
     6961 ctcaatcctt tcgatcaaca atgcacccaa atcctcactc ttcaacgcac acgaacaata
     7021 aaccgcatca ggctcgcgct ccaaatagcg gaggatgtgc aactcgtcca actgcctcat
     7081 cacgaccacc gtcttatcaa tatgccgctg catcctgccc gtaaccgtat ttagcagaac
     7141 gaccgcgctg taaaactcaa tctccctgcc gctcaaatgc atcagcatct tttgcgcgtt
     7201 ggcaaggttc atcggcttgc cccactgcct gccgtcgcac cacgccacct ggtccgcacc
     7261 gacaatcaac gcctcgggga aacgtccggc caacgaccgc gccttaccct cggcaaggcg
     7321 caatgccgtc tgaggggcgg attcccccaa catcggcgtt tcgtcaaaat cgggggatgc
     7381 cgcctgaaag gcaatgccga gcctttccat ctgttcgcgg cggaaaaccg aactcgtacc
     7441 caaaatcaaa ggcagttcca aacccatccc atcctcctta ccgttgaaaa cacgcccgaa
     7501 ggggcggtaa aatccagcca tgcgccgaaa cacggatacc cgccttcggc gtaccgcaac
     7561 atttttctta aaaatattga cgttagaaca tctaaattat atcatacctc gtttatgtca
     7621 gaccctaatt tgattgaccc ggaaattttt gccgccgaag ggcagaacct gcaaggcagt
     7681 tttctgctgg aagaattgga tgaacgcgtc agttcgcacg attatcccgc cgacaggcag
     7741 accaaaatat cgtttacact gaccggcggt cgcgatcggc tgcaacgcct gttcctcgac
     7801 ctgaacgtca aagccgatat gcccctgatt tgccagagat gtatcaaacc catgccgttc
     7861 atgcttgatg aaagcagccg tatcgtcctg ttttccaacg aagagtcctt ggacgaatcc
     7921 atgcttgccg acgaagaact cgaaggcata ctgattgaaa aagaactcga cgtgcgcaca
     7981 ttggtagaag accaaatcct gatgtccctg cccttttcgc cgcgacacga agactgcggc
     8041 gacaatggga cactggaaga agtcaatcgg gacaaaccca acccctttgc tgttttggca
     8101 ggtttgaaaa gcagttaatt aggacacagt ttatttatct aggagcttga aatggccgtt
     8161 caacaaaaca aaaaatcccc ttccaaacgc ggtatgcacc gttcgcacga cgcgctgacc
     8221 gcgcctgcac tgtctgtcga cagcacaacc ggcgaagtac accgcccgca ccacatctcc
     8281 cccaacggta tgtaccgcgg ccgcaaagtg gtcaaagcca aaggcgaata atccctattc
     8341 gactgactga aaaagccaga acattgccat gcaattactg gctttttttg cattggacgc
     8401 accatccgtc caaactttcg ccatacgtca acacacaggg gcaaagcgtt ccgtataata
     8461 ccccgtgaaa atattccaaa agccccaacc accaaggaaa ttccgatgaa acagaaaatc
     8521 tggtacacct acgatgacat ccaccgcgtc atcaaagcat tggcagaaaa aatccggaac
     8581 gccgacatca aatacgatgc catgattgcc atcggcggcg gcggctttat tccggcacgt
     8641 atgctgcgct gttttctgga aattccgatt tatgccgtaa ccaccgccta ttacgacagc
     8701 gacaacgaag gacaggttac agaagaagtc aaaaaagtcc aatggctcga ccccgttccc
     8761 gaagccctgc gaggcaaaaa cgtccttgtt gtcgatgaag tggacgacag ccgcgtaacc
     8821 atggaatttt gcctgacaga gctgcttaaa gaagacttcg gcaccatcgg cgtcgccgta
     8881 ctgcacgaaa aaatcaaagc caaagcaggt aaaatccccg aaggcattcc ctatttcagc
     8941 ggcatcaccg tagaagactg gtggatcaac tatccatggg acgcactcga catcgacgaa
     9001 cacaaccgcc ttgccgaggc cggccgaggc tgaccctttc agacggcata ttttccgaac
     9061 cgatgccgtc tgaagcccgc acgacccctg ccgcagaccg aaaacctacc ggagaaccct
     9121 atgattacac ttgccgtaga tgccatgggc ggcgaccaag gacttgccgt taccgtaccc
     9181 ggcgcaaccg cattcctcca agcacacccc gatgtccgcc tgattatgac cggcgacgaa
     9241 acgcaactgc gccaagccct gaccgcggca ggcgcaccga tggaacgcat cgacatctgc
     9301 cacaccgcac aaatcgtcgg catggacgaa gcgccgcaat ccgccctgaa aaacaaaaaa
     9361 gactcctcca tgcgcgttgc catcaaccaa gttaaagaag gcaaagccca agccgccgta
     9421 tccgcaggca acacgggcgc actcatggca accgcacgtt tcgtcctcaa gaccataccc
     9481 ggtatcgaac gccccgccat cgccaaattc ctcccgtccg acaccgacca tgttaccctc
     9541 gccctcgacc ttggcgcgaa cgtcgactgc acgcccgaac agctcgccca atttgccgtt
     9601 atcggcagcg aactcgtcca cgcactccat cctcaaaaag gacagccgcg cgtcgggctg
     9661 gtcaacgtcg gcacggaaga catcaaaggt acggacaccg tcaaacaaac ctacaaactg
     9721 ctgcaaaaca gcaaactcaa ctttatcggc aacatcgaaa gcaacggcat cctctacggc
     9781 gaagcagatg tcgtcgtcgc cgacggcttt gtcggcaacg tcatgctcaa aaccatcgaa
     9841 ggcgcggtca aattcatgag cggagccatc cgccgcgaat tccaaagcaa cctgttcaac
     9901 aaacttgccg ccgttgccgc cctacccgcc ctcaaagggc tgaaaaacaa actcgatccg
     9961 cgcaaattca acggggccat cctgctcggg ctgcgcggca tcgtcatcaa aagccacggc
    10021 ggcacagacg aaaccggttt ccgctacgca ctcgaagaag cctaccacga agccaagtcc
    10081 gccggccttt ccaaaatcga acaaggcgtt gccgaacaac tcgccgcact cgaaactgcc
    10141 aaagccgtcc aaaacgaaaa tgccgacggt ctgtaacaca cacgatgccg tctgaacgcc
    10201 cccgcccctt tcagacggca tccgcccgca ccaaacctgc gggcgcggac ggcgatgcgc
    10261 ctgtccggca cttcccaaat acttccctgt aaaataagga gtatttgaaa aatgaagaca
    10321 ttagaaaaac ggatgaaagc tctagacaaa cagatgatga agttcggaaa atcccttgaa
    10381 ggcaggcttg atgcccgtct gattgaatcc gcattggatt atattcatta ttcggaacgt
    10441 tttttggctt ttgaaatcct gtgtacttat atcgaagatt tcgatgtccg gctgacggaa
    10501 caagaatccc gggaaatttc ttttatcaac aaggaatttg agatagaaag cacgtccgat
    10561 taaccaataa agccaatggg ttgataaaca tgaaaacatc gacggtcgtt tttggcggat
    10621 tttttatggc agacaacgga gagcgaatcc aaatccccgt tttggaaaat cctgacatta
    10681 gggaaatcaa tcactttttt ccgtatcaaa ttttgagaaa aaagccggcg tccttgtttt
    10741 cagaatcatc cccgagccgg aatttggcga taccgaatta actatctatt ttgaaaaagg
    10801 atattatttg ccaatgattc aaacaatttt agaagacggg gatattgaag tcaaaaacct
    10861 taaaacagaa aattacagcg ggaaggcaag ggaaatcttg ggagattcct accctattga
    10921 gcatatctct aaaaatattt ctacaattca aaatataatt tctgaattta tgatacaaaa
    10981 acaaacaccg gcaccaataa tttgatggaa aaccaacaat caaaatgccg tctgaagcat
    11041 gtttggtttt cagacggcat ttccttcgct taaaacagcg tatcggcaac cccgccctgc
    11101 ctgtccacgg caatctgcat ctgaaaacca tctgtatccc aaaccacacc cccatccctg
    11161 tttccgtcat ttgcaccctg tccgtattgg gcaatcatcg gtttttcgct tacaatagcc
    11221 gaatctgaac caactctcta aaaaggccgt tcccatgcag tatgcaaaaa tttccggcac
    11281 gggcagctat cttcccgcca accgcgtcag caatgacgac cttgcccaaa aggtagatac
    11341 ctctgacgag tggattaccg cgcgtacggg catcaagttc cgccatattg cagccgaaaa
    11401 tgagaaaacc agcgatcttg ccgccgaagc agcccgccgc gcactggatg caaccggatt
    11461 agacagcggc gaaatcgatt taattattgt ggcgacggca acgccggata tgcagtttcc
    11521 ttctaccgcc accatcgtcc aacaaaaatt gggcatcacc aacggctgcc ccgcgtttga
    11581 cgtacaggcg gtgtgtgcgg gctttatgta cgcgctgacc acggcaaacg cctacattaa
    11641 aagtggcatg gcgaaaaacg cgctggtcat cggcgcggaa accttcagcc gcattgtaga
    11701 ctggaacgac cgcacaacct gcgtattgtt cggcgacggt gcgggcgcgg tggttttaag
    11761 cgcggcggac aaaccgggca tcatccacag caaactcaag gccgacggca attatctgaa
    11821 actcttaaac gtccccgggc aaatcgcctg cggcaaagtt tccggttcgc cgtacatttc
    11881 gatggacggt cccggcgtgt tcaagtttgc cgtcaaaatg ctgtccaaaa tcgccgatga
    11941 cgttatcgaa gaagccggtt acaccgccgc tcaaatcgac tggattgttc cccatcaggc
    12001 aaaccgccgc attatcgaat cgaccgcgaa acatttgggg ctgagtatgg acaaagtcgt
    12061 cctgaccgtc caagaccacg gcaacacgtc cgccgcatcg attccgctgg ctttggatac
    12121 aggcatccgc agcggacaaa tcaaacgcgg tcaaaacctg ctgctcgaag gcatcggcgg
    12181 cggtttcgcg tggggcgcgg tgctgttgca atattgaacc cgatgccgtc tgaaacaggc
    12241 tttcagacgg catttcccat atcatgaagc ggcaggcttt cttcaaactg atggcgtgtg
    12301 cggcatttct gtctgccgtt tcgctgcgcc tccccgtatt gggcgcgtgt tacgcaatat
    12361 tgtccctcta tgcgtttgca ctttacggca tcgacaaacg gcgtgccgtg cggggaaaac
    12421 gccgcattcc cgaacaccgc ctgctgctgc ctgccttgtt cggcggttgg gcgggcgcat
    12481 acttgggcag caggatattc aggcataaaa cggcgaaaaa gcgttttgtt gtgctgttcc
    12541 gtctgactgt ttcgggcaat gtcctggcga cctgcatcct gattgattat ttcgttccgc
    12601 ccgaactttt tgtcaaactc gggcaacatc tctgacaatc caaccaaaca aaggaatacc
    12661 atgtcttttg ccttcttttt tcccggacaa ggttcccaaa gcctcggtat gatgaacggc
    12721 tttgccgaac acgccatcgt caaaaacacc tttgccgaag cctccgccat attggggcag
    12781 gacttgtggg cgatgattaa cggcagcgat gccgaaatca tcggacaaac cgtcaatacc
    12841 cagcccatca tgctcgccgc cggcgttgcc gtttaccgcg cctatttaga agcgggcggc
    12901 aaaacgcctg ccgccgttgc cggacacagc ctcggcgaat acaccgcact cgttgccgcc
    12961 ggcgcattga attttgccga cgcggtcaaa ctcgtgcgcc tgcgcgccga actgatgcag
    13021 tccgccgtac cgcaaggcgt gggcgcaatg gcggcgattc tcggcttgga agatgagcag
    13081 gttaaagcca tttgtgccga agccgcccaa ggcgaagtgg tcgaagccgt caacttcaac
    13141 tcacccggac aaatcgtgat tgcaggcaac gccgccgccg tcgagcgcgc catgaccgcc
    13201 gccaaagaag ccggtgccaa acgcgccctg ccgctgcccg tgtccgtacc ttcccattgc
    13261 agcctgatga aacccgccgc cgaaaaactt gccgaagccc tgaaatcggt tgtaatcaaa
    13321 cagccgcaaa tccgcgttat ccacaacgcc gacgttgccg cctacgatga tgccgacaaa
    13381 atcaaagacg cgctcgtccg ccagctttac agccccgtac gctggacgga aaccgtcaac
    13441 gccctcgttt cagacggcat tgccgaatcc gccgaatgcg gcccgggcaa agtgttggca
    13501 ggcttggcaa aacgcatcaa caaagccgcc gcgtgcagcg cactgaccga tgccggacag
    13561 gttgccgcct ttatcgaagc gcactgactt cgttctgcaa aaagcagcct gccctcttca
    13621 ggctgctttt catgtccgaa cggcggcagc cccgtattta cgctataatc catcccgacc
    13681 aaaccaccga cagcggctgc cgttgcagtt cccgccctac cgatatgata gaaaaactga
    13741 ctttcggact gtttaaaaaa gaagacgcgc gcagctttat gcgcctgatg gcatacgtcc
    13801 gcccctacaa aatccgcatc attgccgccc tgattgccat tttcggcgtt gccgccaccg
    13861 aaagctacct tgccgccttc atcgcccccc tgattaacca cggcttttcc gcacccgccg
    13921 cgccgcccga gctgtctgcc gccgccggca tcatttccac cctgcaaaac tggcgcgaac
    13981 agtttaccta tatggtttgg gggacggaaa acaaaatctg gaccgtcccc ctcttcctca
    14041 tcatcctcgt cgtcatccgt ggcatctgcc gctttaccag cacctatctg atgacttggg
    14101 tctccgtgat gaccatcagc aaaatccgca aagatatgtt tgccaaaatg ctgacccttt
    14161 cctcccgcta ccatcaggaa acgccgtccg gcaccgtact gatgaatatg ctcaacctga
    14221 ccgaacagtc ggtcagcaac gccagcgaca tcttcaccgt cctcacgcgc gacacgatga
    14281 ttgttaccgg cctgaccatc gtcctgcttt acctcaactg gcagctcagc ctcatcgtcg
    14341 tcctgatgtt ccccctgctc tccctgctct cgcgctacta ccgcgaccgt ctgaaacacg
    14401 tcatttccga ctcgcaaaaa agcataggca cgatgaacaa cgtgattgcc gaaacccatc
    14461 agggacaccg tgtcgtcaag ctgttcaacg ggcaggcgca ggcggcaaac cggttcgacg
    14521 cggtcaaccg caccatcgtc cgcctcagca aaaaaatcac gcaggcaacg gcggcacatt
    14581 ccccgttcag cgaactgatc gcctcgatcg ccctcgccgt cgtcatcttc atcgccctgt
    14641 ggcaaagcca aaacggctac accaccatcg gcgaatttat ggcattcatc gtcgccatgc
    14701 tgcaaatgta cgcccccatc aaaagccttg ccaacatcag catccctatg cagacgatgt
    14761 tcctcgccgc cgacggcgta tgtgcatttc tcgacacccc gcccgaacag gacaaaggca
    14821 cgctcgcgcc gcagcgtgtc gaagggcgca tcagcttccg caacgtcgat gttgaatacc
    14881 gttcagacgg catcaaagcc ctcgacggct tcaacctcga catcagacaa ggcgaacgcg
    14941 tcgccctggt cggacgttcc ggcagcggca aatccaccgt cgtcaacctg ctgccccgct
    15001 ttgtcgaacc gtctgccggc aacatctgca tagacggtat cgacatcgcc gacatcaaac
    15061 tcgactgcct gcgcgcccaa tttgccctcg tctcccaaga cgtattcctg tttgacgaca
    15121 ccctgtttga aaacgtccga tacagccgtc ccgacgcggg cgaagccgaa gtcctatccg
    15181 ccctccaagc cgctaacctg caaagcctga tcgacagctc cccgctcgga ctgcaccagc
    15241 ccatcggatc gaacggcagc aacttatccg gcggacagcg gcaacgcgtc gccattgccc
    15301 gcgccatttt aaaagatgcg ccgatactgc ttctggacga agccaccagc gcattagaca
    15361 acgaatccga acgcctcgtc caacaggcgc tcgaacgcct gatggaaaac cgcaccggca
    15421 tcatcgtcgc ccaccgcctg accaccatcg aaggagccga ccgcatcatc gtaatggacg
    15481 acggcaaaat catcgaacaa ggcacacacg aacaactgat gtcccaaaac ggctactaca
    15541 cgatgttacg caatatctca aacaaagatg ccgccgtccg gacggcataa acaaaatgcc
    15601 gtctgaaatg gtacaatcgc cccgacccct tcagacggca tcacatccgc cgacccatcc
    15661 gattatcttc aatcactgta aaacccatta tgacccaaga caaaatcctc atcctcgact
    15721 tcggttctca agttacccag ctgattgccc gccgcgtgcg tgaagcccac gtttactgcg
    15781 agctgcattc cttcgatatg cctttggacg aaatcaaagc cttcaacccc aaaggcatca
    15841 tcctttccgg cggccctaat tccgtttacg aatccgacta tcaagccgat accggtatct
    15901 ttgacttggg cattccgatt ttgggcattt gctacggcat gcaatttatg gcgcaccact
    15961 tgggtggtga agtgcaaccc ggcaaccagc gcgaattcgg ttacgcgcaa gtcaaaacca
    16021 tcgacagcga gctgacacgc ggcattcaag acgatacgcc aaacacgctc gacgtatgga
    16081 tgagccacgg cgataaagtt tccaaactgc ctaccggttt caccgtcatc ggcgacacgc
    16141 cgtcctgccc gattgccatg atggaaaacg ccgaaaaaca attctacggc atccaattcc
    16201 accccgaagt aacccacacc aaacaaggcc gcgccctgtt gaaccgcttt gttttggata
    16261 tttgcggcgc acaaccgggc tggacaatgc cgaactacat cgaagaagcc gttgccaaaa
    16321 tccgcgaaca agtcggcagc gacgaagtga ttttaggctt gtccggcggc gtggactctt
    16381 ccgtagccgc cgcgctgatc caccgcgcca tcggcgacca actgacctgc gtgttcgtcg
    16441 atcacggttt gttgcgcctg aacgaaggca aaatggtgat ggatatgttc gcccgcaact
    16501 tgggtgtgaa agtgattcac gtcgatgccg aagggcagtt tatggcgaaa ctcgccggcg
    16561 ttaccgaccc tgagaaaaaa cgcaaaatca tcggcgcgga atttatcgaa gtatttgatg
    16621 ccgaagagaa aaaactcacc aacgccaaat ggctggcgca aggtacgatt taccccgacg
    16681 taatcgaatc cgcaggcgcg aaaaccaaaa aagcccacgc catcaaatcc caccacaatg
    16741 tcggcggcct gcccgaaaat atgaagctca agctgcttga gccgttgcgc gatttgttca
    16801 aagacgaagt gcgcgaattg ggcgttgctt tgggcctgcc gcgcgaaatg gtgtaccgcc
    16861 atcccttccc cggcccgggt ttgggcgtgc gcatcttggg cgaagtgaaa aaagaatacg
    16921 ccgacttatt gcgtcaggcg gacgatattt tcatccaaga attacgcaac accaccgacg
    16981 aaaacggcac atcttggtat gacctgacca gccaggcatt tgccgtattc ctacctgtga
    17041 aatccgtcgg cgtaatgggc gacggacgca cttacgacta cgttgtcgca ctgcgcgccg
    17101 tcatcaccag cgactttatg accgcacatt gggctgagtt gccttactca ctgctcggac
    17161 gcgtgtccaa ccgcatcatc aacgaagtca aaggcatcaa ccgcgtgatg tacgatgtca
    17221 gcggcaaacc gcctgccacc atcgagtggg aataaccagc aaacagggct gcaccgtccg
    17281 gcgcagtctt tcgattatcg gaaaaaagga aaaaatatga gcacacaaga tttaagcggc
    17341 aaaatcgctt tggtaaccgg cgcatcgcgc ggtatcggtg cagcaattgc cgacacactg
    17401 gcggcagccg gtgccaaagt catcggtacg gcgaccagtg agagcggtgc ggcggcgatt
    17461 agcgagcggt tggcgcaatg gggcggcgaa ggccgcgtat taaattccgc cgaacccgaa
    17521 accgtcgaaa acctgattgc cgacatcgaa aaaacgttcg gcaaactcga tattctggtc
    17581 aacaacgccg gcatcacccg cgacaacctc ctgatgcgca tgaaagaaga agagtgggac
    17641 gacatcatgc aggtcaacct caaatccgtg ttccgcgctt ctaaagccgt cttgcggggt
    17701 atgatgaaac aacgttccgg ccgcatcatc aacattacct ccgtcgtcgg cgtgatgggc
    17761 aatgccgggc aaaccaacta tgccgcggca aaagcaggct taatcggttt ctccaaatcc
    17821 atggcgcgcg aagtcggcag ccggggcatt accgtcaact gcgtcgcccc cggctttatc
    17881 gataccgata tgacccgcgc cctgccggaa gaaacccgcc aaacctttac cgcccaaacc
    17941 gccttgggca gattcggcga cgcgcaagac atcgccgatg cagtcttgtt cttggcttcc
    18001 gatcaggcaa aatacattac cggtcaaacg ctgcacgtca acggcggcat gctgatgccc
    18061 tgacggacag gctttttaac catgccgtct gaaaaggttt cagacggcat ttgcattctc
    18121 aggcaaaatg aacacacgcc acaccccgcc ctgcccctgc ggatcaggca caagctgaga
    18181 cctttgcaaa actcctttcc ctcccgacag ccgaaacccc aacacaggtt ttcggatgtt
    18241 ttcgccccaa atacctccta attttaccca aataccccct taatcctccc cggatacccg
    18301 ataatcaggc atccgggctg ccttttaggc ggcagcaggc gcacttagcc tgttggcggc
    18361 tttcaaaagg ttcaaacaca tcgctttcag atggctttgc gcactcactt tgagcagccc
    18421 gaaataggct gcccgagcat agcggaa
//
LOCUS       DAWFIE010000036        18416 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWFIE010000036 DAWFIE010000000
VERSION     DAWFIE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.036"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(306..4475)
                     /locus_tag="WGQ71_001148"
     CDS             complement(306..4475)
                     /locus_tag="WGQ71_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ0707163.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPAPPAKKRRPLLKLSAVLLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMHRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRINPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTLWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNINRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGTLKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNGKGK"
     gene            complement(4530..6359)
                     /locus_tag="WGQ71_001149"
     CDS             complement(4530..6359)
                     /locus_tag="WGQ71_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ0707164.1"
                     /translation="MMIKPTALLLPALFFFPHTYAPAADLSENKAAGFALFKNKSPDT
                     ESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAPDNVKTM
                     LRSKGYFSSKVSLTEKGGAYTVHITPGPRTKITNVGVTILGDILSDGNLAEYYRNALE
                     NWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADLNVVVDS
                     GRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHYSGASVQ
                     ADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKGYIGSVV
                     WDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVRDRAGID
                     ARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDGKIGTTL
                     GAFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNANVPSGLMFRSGGA
                     SSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDAAANFKR
                     MKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6790..8019
                     /gene="sstT"
                     /locus_tag="WGQ71_001150"
     CDS             6790..8019
                     /gene="sstT"
                     /locus_tag="WGQ71_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ0707165.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIILYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8130..9413
                     /locus_tag="WGQ71_001151"
     CDS             8130..9413
                     /locus_tag="WGQ71_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0707166.1"
                     /translation="MFKRSVIAMACIVALSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     KETEVKEDAPQAGSQGQDVPSKQGSQDMAAVSAENTGNGGSATTDKPKNEDEGPQNDM
                     PQNSAESANQTGNNQPADSSDSAPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLT
                     HCKGDSCNGDNLLDEEAPSKSEFENLNESERIEKYKKDGKSDKFTNLVATAVQANGTN
                     KYVIIYKDKSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGN
                     IFAPEGNYRYLTYGAEKLSGGSYALSVQGKPAKGEMLAGTAVYNGEVLHFHTENGRSY
                     PTRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGNWTENGGGDVSGR
                     FYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9489..9704)
                     /locus_tag="WGQ71_001152"
     CDS             complement(9489..9704)
                     /locus_tag="WGQ71_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707167.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDDGFIN"
     gene            complement(9697..9912)
                     /locus_tag="WGQ71_001153"
     CDS             complement(9697..9912)
                     /locus_tag="WGQ71_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ0707168.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(9987..11330)
                     /gene="argG"
                     /locus_tag="WGQ71_001154"
     CDS             complement(9987..11330)
                     /gene="argG"
                     /locus_tag="WGQ71_001154"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ0707169.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRTQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIKPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAITGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLALGEGSVLPQLGNKK"
     gene            11657..11797
                     /locus_tag="WGQ71_001155"
     CDS             11657..11797
                     /locus_tag="WGQ71_001155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707170.1"
                     /translation="MQTAALKQTLPEKRKLDPLPNPLPWEREQITAPSTFSDGIGKET
                     HL"
     gene            11794..12594
                     /locus_tag="WGQ71_001156"
     CDS             11794..12594
                     /locus_tag="WGQ71_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ0707171.1"
                     /translation="MNAFNLTIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYVGRFNQTKRGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            12634..13620
                     /locus_tag="WGQ71_001157"
     CDS             12634..13620
                     /locus_tag="WGQ71_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ0707172.1"
                     /translation="MQYRIRRENEAPEAKNTGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14277..14539
                     /locus_tag="WGQ71_001158"
                     /pseudo
     CDS             14277..14539
                     /locus_tag="WGQ71_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14577..14951)
                     /locus_tag="WGQ71_001159"
     CDS             complement(14577..14951)
                     /locus_tag="WGQ71_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707173.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVTYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
     gene            complement(15124..15480)
                     /locus_tag="WGQ71_001160"
     CDS             complement(15124..15480)
                     /locus_tag="WGQ71_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ0707174.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(15542..15658)
                     /locus_tag="WGQ71_001161"
                     /pseudo
     CDS             complement(15542..15658)
                     /locus_tag="WGQ71_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(15745..15957)
                     /locus_tag="WGQ71_001162"
     CDS             complement(15745..15957)
                     /locus_tag="WGQ71_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707175.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            complement(16033..16392)
                     /locus_tag="WGQ71_001163"
     CDS             complement(16033..16392)
                     /locus_tag="WGQ71_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0707176.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(16389..16607)
                     /locus_tag="WGQ71_001164"
     CDS             complement(16389..16607)
                     /locus_tag="WGQ71_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707177.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNIL"
     gene            complement(16628..16834)
                     /locus_tag="WGQ71_001165"
     CDS             complement(16628..16834)
                     /locus_tag="WGQ71_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707178.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKDLPIR"
     gene            complement(16800..17000)
                     /locus_tag="WGQ71_001166"
     CDS             complement(16800..17000)
                     /locus_tag="WGQ71_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707179.1"
                     /translation="MASDLLSKQTSDKILGYQTEHRRVRYDINNNIYVLANPKTFKIK
                     TMFKPNLGKKYYDGEFKKDMGN"
     gene            complement(17360..17704)
                     /locus_tag="WGQ71_001167"
     CDS             complement(17360..17704)
                     /locus_tag="WGQ71_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707180.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(17742..18065)
                     /locus_tag="WGQ71_001168"
     CDS             complement(17742..18065)
                     /locus_tag="WGQ71_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707181.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(18068..18298)
                     /locus_tag="WGQ71_001169"
     CDS             complement(18068..18298)
                     /locus_tag="WGQ71_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ0707182.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENTRTGEKTDFKFIENTRTGEKTDFKFKD"
BASE COUNT         4258 a         4391 c         4861 g         4906 t
ORIGIN      
        1 gcgggaatga cggaaagtgg cgggaatgac ggttcgggca ttccttaaat tacctgtgta
       61 tcgttgtaaa tcttagagat ggcggaatat agcggattaa caaaaaccgg tacggcgttg
      121 ccttgcctta gctcaaagag aatcggttcc gtactatttg tactgtctgc ggcttcgtcg
      181 ccttatcctg atttttgtta attcactata tcgattctgt tcccttgcag gcggacggcg
      241 gcggcggacg gtggaaaagc aggttttcaa atgtccggtt tggcggcgcg ccgtctgaaa
      301 accgcttatt ttcctttgcc gtttccggcg gagtcttttt tgtccgaacc ggagaagcgg
      361 tcgaaacgta tggtgtatgt cagctcgccg cccgacgaac ggctaccgat acgggcaacc
      421 gcctgtatgg cgcgtgtcag ccggtaaatc agtttgacgg actgttccgc gctggagatg
      481 ctgtattcgt agccgatgta gagtttgccg gtcagttgtt tgccgacggt cagcacctgt
      541 tcggcggggt tgagttcgcc ggtttgcgcg ttgcggctgc gcttgctggt aaagcccaaa
      601 tcatccacca gcccgatgcg gtcgttgatt tgcccggcaa gcagcgcgcc tgcggctgcg
      661 gacagggcgg cattgtcgcc gctgctgccg ctgccggcgc ggttgaggat gagccaggag
      721 agcttgtctt tttcactcat cggttcgttt gccgtcagcg taatgcgcgg actgttgagg
      781 ctgcccaata tttccacgcc cgcaccgacg ggggaaaggc ggcgttcggc gcggatgttg
      841 aggttggggt cgttgagcgg gccgacaaag gagaccgtgc ctttggtaat gtccaaatcc
      901 tgcccgtatg ccttataacg ccctttgatg acgcggaccg tgcccacgcc ccgcacgctt
      961 ccgcccgatt gggcggtcag ggtcagtttg ccgcctatgg taacgtccgc gccgtagccg
     1021 gcgaagcgga tgccgtcatt gaggtctaaa gtcaggttca tattgacggg gagcggtgcc
     1081 gccgcctctt ttttaacttc gcctaatacg acgacatcgt cgccgacgga cggcatcgag
     1141 gatttttgcg aaccgaacag cccctgatcc gttttaatca tcccggtaac ggatatgcct
     1201 ttttgcggcg aatagcgcag gcgggtgttg ccggaaaccg tcaggcggcg gttagggcgg
     1261 gacaggatgc ggtatttgtc gaacaccgcg ccgatatcga catcgggtcc gctgttttcc
     1321 ataccgaccg tgccggagag ttccgccgtt ccttcgtgcc ggaatttcag gctgtcgatt
     1381 acccattttc tgcccgcgat atgcgaacgc agcgagccgt tgtccaagat gatgccttgg
     1441 gtttggttgc ggtaatagag cttgtcgccg ttgatgctgc cgcccaagtg cgggtcggcg
     1501 atgctgccgc cgagggttac ggcggcattc aggctgcctt tgacggtttg tccgacgggc
     1561 aggaagttgc ggaatacttc ggcatcggca acggtcaggt tgagcctgcc gcccaaaggc
     1621 gcggtatcga aagagcggct ttgcccgacg gtgatgttgc cgttgatttt cccgtagttg
     1681 ctgctgccgt tgacggcggc attgacggac ggagagccga cccgtccgcc gatttgcgcg
     1741 gcggcattca ggctgccggt aatgttttgc gcggcggcgg gcagaaaggg cttcaatgtg
     1801 cccaagtcgg gaagggaggc ggtaatcctg ccgccgagcg gtgcatttgc catattgccg
     1861 ccgaaggcgt tgccgatgtc caaatcggcg ttaatccgcc cgaaacgtgc gccgccgtca
     1921 agcaggattc cgatacggtc gttttgaaag cgcgttttca gggaaaatgc gttcaaaccc
     1981 aaagcctgcc cgccgggcaa tacggcatcg ccgctttgcc gattgatatt gaggtagccg
     2041 cgcgcgttgc gcccgtaggc gacatcccag tcgccgttta aaaccagatt gtgttcgaag
     2101 ggcggtttga agaaattgtg caactcggcg atatgcagac ctcgtgcgcc gccttttgcc
     2161 gatatgccgg ttttcctgtc ccaggaaaag tgttgcaggt tgaggctgcc gcccatagcc
     2221 tgccaatttg ccgcacttgc cgccacacgt tccgcaccgg cttcgagcgt catacggttt
     2281 tgcagtttta ggttgaatgc gccgccgatg tcgagaatgc cgatgctgcc cttccatagg
     2341 gtaagttccc tgttgatgcc gcctgaagcg tccaaatcga atttgaacgg tttgccatcc
     2401 agcgtcatgg cggcgtgtgt gcggatgcgg tgctgcacgc ccgtgccgtc cagcatcagg
     2461 tcggcggtat cgacaaccgc cgctccgccc gacagcgaaa ggcggctgcc tttgatgtcg
     2521 gcgcgtatcg ggcggcttgt gtcgggcgaa cctttgagcg tgaaatcgag cgaacggatg
     2581 tctgccgcct tgccgatgtg caggttgcgc gccgcgccgg aaaggtcggt ttcaaaggtt
     2641 cggatgccgc cgtccaaatc gccggaaagg tgtccgcgta catttaaaga ccccgcgagt
     2701 ccgaaaccga aacgggataa atcgggtgcg gtgatgttga ggttgagccg gtcgcctttt
     2761 ttgccgaagc cgccgtctgt tttaataatg ttccgcccca gccgcaaatc gacggcggca
     2821 cgcggaaggt ggcgggactc gtaaacaatg tcggcactgc cggcaatcgg tacgccgttg
     2881 aacgtgccgg gtaaaaaccg cattttgcct gtgaatttct cttttgccag ttcgccggca
     2941 aggtttattg agccgttgat attgcctgcc ggaagttgcg gattgatgcg cgaagggtcg
     3001 aatgcgcggg aacggatgtc cagcttgagc aggcggtctt taaacagctc gagatagcct
     3061 tgcgcggtca ggctgccttg cccggcggcg atgttgacgg tgtcgagcac cagtttccgc
     3121 tgtccgtttg cggggtcgct tgcaatggcg aggctgccgt ccgtgcgcgc cgtgccgatg
     3181 ccgagttgcc aagagatttt gggcgaggcg gtcgtgccac cgatgccgat gctgccgtcc
     3241 aacctgcctt tgaacgcggt ttgcagtacg tcttccgcgc cgacggagtt gatgcctata
     3301 tttaaatcga ggatgtcttt ttcggtgtcg attttgcccg acagcctgat gccgccccgt
     3361 ccgagcaggg cggcggacgt attgccgata tgcaccgtgc cgtcctgccg gatgacaaag
     3421 ccgcctaaaa cctgacggac ggggatgccg ttgcggtcgg caaagccggc tttggtgttt
     3481 tccaaatcga gcgaaccttc cagcgcgatg ccgtctgaaa acgacgggat ggcggtcagg
     3541 tcgaaattca gtccggcatc gggcagggaa ggcacgaagg cggccggatt gatgttgaac
     3601 cctttgacca gtacttcttc caatgtttta tccaatgatt cggcaaacgg gtggatgacg
     3661 gattttcccg agaggcggat attgccgccg tcgatcgcca gttcggcgcg cacatccttc
     3721 aggctgccgc tcagccgagc cgtactgtgt atggttttgc cttcgagtcc gcctttggtg
     3781 taaatggcgg tatcgagggc aaacggtttt ttcaagccga ccgaggccgc ccccgacgaa
     3841 ctgctccacg gcgtgtcggc ggccttcagg tcaaggcggt gtcctttgcg gtcgtaacgg
     3901 tatgaagcat ccagccgttc gagatagacg gtttgtttgt caaaggcttt gcccatgctg
     3961 attttgcccg tctcgaagcg gtcgagatag acggcggcag gcaggtctat gctgtcggga
     4021 aggctgagcg gcgggcgttc ttctttaggc ggagtcggtt tggtaacgat ggcgatgtcg
     4081 ccggcggaaa tttcggtaat gtgcaggctg cggtgcatca gttcggacgg tttccacgcg
     4141 aagcggaagc ggctgatttt aaggtctgca ccctcggttt ctatcgacca gttgtcgccg
     4201 tcgaagccgt cgagcagcgt gcctttgagg ttttgggagg aaatgtttac gccgaaccaa
     4261 gacgggattt ggtacagccc gaagcgtaaa cccgcttccg tgccggcgag ccagccgagg
     4321 aaacatactg ccaaaatcag gacagacagc agtaccgccg acagcttcag cagcgggcgg
     4381 cgtttttttg ccggcggggc agggcggtgt tcagacggca ttttgcgcgt gccgttttcg
     4441 gtcggatcgg tatctgtcgg tgcggtatcg gtcataatgt ctgtttcaaa atagtttgct
     4501 gcaatgccgt ctgaagcggc cgtatcggtt tagaagcgcg ttcccaagct gatgtgccag
     4561 cggattttct tgtcgctgtg cccgtaggcg atgtcgaagg aaaacggcgc gagcgggctg
     4621 aaccagcgca cgcccagtcc cgaaccgtgt ttcagcttca tacgtttgaa attggcggcg
     4681 gcgtcgccca tatcgtggaa caccgcgccg gaaagggtgc gcgtaaacgg cagttggtat
     4741 tccaggctgc ccaccaagag ggcgcgttcg ggcaggaccg atccgttcgg gccggcaagc
     4801 ccgatgctgt cgagttcgta accgcgcacg gaagacgcgc cgccgctgcg gaacatcagc
     4861 cctgaaggaa cgttggcatt gtcgcgggca acggtgtaac ccgcttgtcc gcgtatgatg
     4921 aacgtgccga gttttttgtt ttcgggcgtg aagaaataac ctgcgcgggc agaggtgcgg
     4981 atcagcgcgg tggaggacag gaatgcgccc aaagtcgtac cgattttgcc gtcgaggtaa
     5041 tggccgtttt cgggatgcag cacgttgttg agcagctggc gtttccaaga ggcggtcagc
     5101 atcgtggcgt ggctgttgcc caaatcgaca tccgagccgg ggattttccg gccttctgcg
     5161 agaaactccg cccccagcct ggcatcgatg cccgcgcggt cgcgcacata ccagatgccg
     5221 ccggagaagg cgcgtttttc gaggttttgg gtggtcgaac ggttgtagga gacgttgctt
     5281 gtccagtagt tgccccgata gttgcgcggc tggctgatgc cggcggcaag cgtggtttcg
     5341 tatttgtcca tatcccagac gaccgaaccg atatagcctt tgttgaagag gttgtaatag
     5401 tcgtaggcga ttttgccgcc caaaccgtat tccgaatcga ggcggatgcc ggtttcgagt
     5461 ttgtggcgtt tgacctcggt tacgctgact ttgacgggga cgcggtcgcc ttggaggcgg
     5521 tcgaagtcgg cttgtacgga cgcgccggaa taatgcccgt tttgttcgag cgcctgttgg
     5581 aagtcgagca gcaggtcgag gtcgtagggc gtgcccggtt ggaagcgcgc caggccggag
     5641 acgatttgtt cggggtaacg ctgtgtgccg gtgatttcaa agtcgccgaa ggcgatgggg
     5701 cggccgctgt ccacgacgac gttcaaatcg gcggtggcgg tatcggggtt gacggccgcc
     5761 cgggtgttgc cgagcttggc aagcgggtag gctttgcgcg ttaccgcgcc gaggacggaa
     5821 gttttgctgt tttcccaact gtcctgatcg aaatcgctgc ctaccggctg ctgccagttt
     5881 tccagcgcgt tgcggtagta ttcggcgagg ttgccgtctg aaaggatgtc gccgaggatg
     5941 gtgacgccga cgttggtgat tttggtgcgc ggccccggtg tgatgtgcac cgtataagcc
     6001 ccgccttttt ccgtcaggct gactttgctg ctgaaataac ctttgctgcg gagcatcgtt
     6061 ttaacgttgt ccggtgcttc ttcggcgagg aagcctgtct gttccttgtc caatacttct
     6121 tcctgctgct gcgtgatgag cggcaggtgt tcttcgacca tatctttgat ttcactgtcc
     6181 tgcgtgtcga tgcggacggg gaatttgggt tttaatttga ctgattcggt gtcggggctt
     6241 ttgtttttga acaatgcgaa acccgccgcc ttgttttcgg aaaggtcggc ggcaggcgcg
     6301 tatgtgtgcg gaaagaaaaa taaagccggc aggagcaggg cggtcggttt aatcatcatg
     6361 gtacgggtgt cgtgcactcg gtgtggcaga tttcgattct aacactgttc ggacagggtt
     6421 tcgaacggct gtttttgtgt tgttcggacg gggcgggaca cttcagacgg catactgccg
     6481 ccggtatttc ggaggttgcg gcccgccgga aatgtatctg cttgttttaa ggtattttgt
     6541 ttccaatccg catatggaaa acaggctgcg tttcggaata gtgccggaaa ataggcagga
     6601 ttaaataaat ttattttcgt tcaataaatt aaaccgttgc gccgaatgat aaagcaaagc
     6661 ctgtccggca ctgcataggg caaatcaacg caaccttaaa acacagccga aaaataaaaa
     6721 aaaacacaga gaatgagttt tcttttaaaa tacaacattt tttaacatat cacaacataa
     6781 ggaagaatta tggcgttcgg caaatcctta tttcatgcga tcggccgcgt cagcctggtc
     6841 aggcagattg ccgccggttt ggcgttgggc atcgtaatcg gttcggtttc cccgcaactg
     6901 ggcttggcgg caggcttgtt cggcagcctg tttgtcggcg cgctcaaagc ggtcgcgccg
     6961 gttttggtat ttattttggt ggcggccaca atcgcgcagc accaaaaagg caacaaggcg
     7021 catatcaggc cgattatcat cctttacctc atcggtacgt tttccgcagc cctgaccgcc
     7081 gtcatcgccg gtatggtttt cccgacgcac attgttttgg cgggcgcggg cgatgtgtcc
     7141 gccgcgccgc cttccggcat tgtggaagtg ttgaaatcgc tgctgatgaa cctggtcgcc
     7201 aacccgatta acgcgattgc caatgccaac tatatcggca ttttggcttg ggctttggtt
     7261 ttgggcgcgg cgttgcggaa tcacggttcg gacgttacgc ggcaggtcgt tgccgatttg
     7321 gcggaagcgg tttccaccgt cgtgaaatgg attatccgtt ttgccccttt gggcattttc
     7381 gggctggtgt catccacaat cgcggagacg ggtttcggcg cgctggcggg ttatgcgaag
     7441 ctgcttgcgg tgctgttggg ctgtatggcg tttatcgcgc tagtggtcaa tcccgccatc
     7501 gtgtggtgga aaatccgccg caatccttat ccgctggtgt ttacctgcct gcgcgaaagc
     7561 ggcgtgtatg ctttctttac ccgttcttcc gccgccaata tccccgtgaa tatggctttg
     7621 gcgaaaaagc tggggctgca cgaagacact tattcgattt ccatcccgtt gggggcgacc
     7681 gtcaatatgg gcggcgcggc gattacgatt accgttttgg ctatggcggc ggcgcacacc
     7741 caaggcatac aggttgattt tgccaccgcg ctactgttga gcctggtggc gacggtcagc
     7801 gcgtgcggtg cgtccggcgt ggcgggcggt tcgctgctgc tgattccgct ggcgtgcagc
     7861 ctgttcggca tcagcaacga tgtcgctatg caggtggtcg ccgtcggctt tatcatcggc
     7921 gtgattcagg attcggcgga aacggcgttg aattcttcaa ccgacgtttt atttaccgct
     7981 gccgccgatt tgggccgaca gagaaaccgg gcggaataaa ccaagctatg ccgtctgaag
     8041 cccgtttggc gttcagacgg catattttac aattttatgg gcatatggcg gaaacaatac
     8101 cggctgcctt gaatatgaag gggatgatga tgtttaaacg cagtgtgatt gcaatggctt
     8161 gtattgttgc cctttcagcc tgtgggggcg gcggtggcgg atcgcccgat gttaagtcgg
     8221 cggacacgcc gtcaaaaccg gccgctcctg ttgttgctga aaaagagaca gaggtaaaag
     8281 aagatgcgcc acaggcaggt tctcaaggac aggacgtgcc atccaaacaa ggcagtcaag
     8341 atatggcggc agtttcggca gaaaatacag gcaatggcgg ttcggcaaca acggacaaac
     8401 ccaaaaatga agacgaggga ccgcaaaatg atatgccgca aaattccgcc gaatccgcaa
     8461 atcaaacagg gaacaaccaa cccgccgatt cttcagattc cgcccccgcg tcaaaccctg
     8521 cacctgcgaa tggcggtagc aattttggaa gggttgattt ggctaatggc gttttgattg
     8581 atgggccgtc gcaaaatata acgttgaccc actgtaaagg cgattcttgt aatggtgata
     8641 atttattgga tgaagaagca ccgtcaaaat cagaatttga aaatttaaat gagtctgaac
     8701 gaattgagaa atataagaaa gatgggaaaa gcgataaatt tactaatttg gttgcgacag
     8761 cagttcaagc taatggaact aacaaatatg tcatcattta taaagacaag tccgcttcat
     8821 cttcatctgc gcgattcagg cgttctgcac ggtcgaggcg gtcgcttccg gccgagatgc
     8881 cgctgattcc cgtcaatcag gcggatacgc tgattgtcga tggggaagcg gtcagcctga
     8941 cggggcattc cggcaatatt ttcgcgcccg aagggaatta ccggtatctg acttacgggg
     9001 cggaaaaatt gtccggcgga tcgtatgccc tcagtgtgca aggcaaaccg gcaaaaggcg
     9061 aaatgcttgc tggcacggca gtgtacaacg gcgaagtgct gcatttccat acggaaaacg
     9121 gccgttcgta cccgaccaga ggcaggtttg ccgcaaaagt cgattttggc agcaaatctg
     9181 tggacggcat tatcgacagc ggcgatgatt tgcatatggg tacgcaaaaa ttcaaagccg
     9241 ccatcgatgg aaacggcttt aaggggaatt ggacggaaaa tggcggcggg gatgtttccg
     9301 gaaggtttta cggtccggcc ggcgaggaag tggcggggaa atacagctat cgcccgacag
     9361 atgcggaaaa gggcggattc ggcgtgtttg ccggcaaaaa agagcaggat tgatgttgat
     9421 gccgtctgaa gcgagggcgt tcagacggca ttgtgatgtt tgggtcgcgg gtccggttca
     9481 agtccgcatc agttgataaa cccgtcgtct ttcattttct tttcggaaaa atattgaccg
     9541 cataccagta aggcggcgag gatgatgaca aacagccccg ccatagtcag tgtgattttg
     9601 aagaacaatt ccgcttcgag aaaagaaaac caaagttgca caatcagcag cagcacccag
     9661 ccggcaaaca gtccgagaca ggcataaacg ccgagcttaa acacggtccc gctccaaaat
     9721 gacgacgtag gcctcgcgtt tgaaaaacaa cagcatacgg cgcaaatctt tttccatcgt
     9781 tacaacccgc cagccttcgg gggtttgttt gttgaggaac gcgctgaaat tgacggggtt
     9841 gacctttgcc gcgccgaaaa acaggctgga caacaggctt tcctgataaa tgacgacttt
     9901 gtattctttc ataggattcc ttttaacata agggatcatc ttttcagacg accccttaag
     9961 ttgctaatga tgcaaaacag caaaccttat ttcttattgc ccaactgcgg caataccgaa
    10021 ccttcgccca atgccaacaa accgctttgc gagtaaatgc ccagtttggc gcgggtgtcg
    10081 gtaatgtcga ggttgcgcat agtcagttgt ccgatacggt cgagcggtgt gaacgctgcg
    10141 tcttcgactt tttccatact caggcgttcg ggttggtagg tcaggttggg cgattcggtg
    10201 ttcagaatcg agtagtcgtt gccgcgacgc aattcgaggg ttacttcgcc ggtaatggct
    10261 ttggcaaccc agcgttgggc ggtttcgcgc aacatcaggg cttggctgtc gaaccagcgg
    10321 ccttggtaga gcaaacgtcc gaggcgcagg ccgttgatgc ggtattgttc gatggtgtct
    10381 tcgttgtgga taccggtcac caaacgctcg taggcgatgt ggaacaacgc cattcccggg
    10441 gcttcgtaga tgccgcgcga tttggcttcg atgatgcggt tttcgatttg gtcgctcata
    10501 cccagaccgt ggcggccgcc gatgcggttg gcttcgagga agagttcgac gggatcagcg
    10561 tattctttgc cgttcagtgc aaccggcacg ccttcttcaa agcgcacgct gacttcttcg
    10621 ggtttgattt cgacgttttc gtcccagaag gcaacgccca taataggttt gacgattttg
    10681 atgcccgagt tcaaaaattc caaatctttg gcttcgtggg tcgcgccgag catattggaa
    10741 tcggtggaat aggctttttc caccgacatt ttgtagttga aaccgttggc aatcaaaaat
    10801 tcgctcattt catgacggcc gccgagttcg tcgataaatt gctggtcgag ccagggtttg
    10861 tagattttca gcgcgggatt ggtgagcaaa ccgtagcggt agaaacgctc gatgtcgttg
    10921 cctttgtagg tgctgccgtc gccccaaata ttcacatcgt cttctttcat ggcggaaaca
    10981 agcatagtgc cggttacggc gcgacccaga ggcgtggtgt tgaagtaggc gatgccgccg
    11041 gtggaaacgt ggaacgcgcc gcattggatg gcggcgatgc cttcgtgtgc cagctgtgtg
    11101 cggcagtcaa tcaggcgggc gttttccgca ccgtattcca ttgctttttt gggaatggca
    11161 ttgtagtcgt cttcgtcggg ctggccgagg ttggcagtgt aggcataagg tagcgcgcct
    11221 ttgagtttca tccacaacag cgcggcagag gtatcaagac cgccggagaa ggcgatgccg
    11281 actttctgac cgacggggag gttttgtaaa atagtatggt tttggctcat aagggtatcc
    11341 ttcggacttg gaaaaaggag ttcgtcgtgt ttccttttcg attgaaaccc cgccacttgg
    11401 acatctgtcc ttcggggcgg tagaatcaga ctttatttgg gaggggcgta atcccttcca
    11461 aatcaggacg gcacataggg cggtgcttta tgtgtcgtcc cgtgtgttga aacattatct
    11521 tataaatata acccattgtc agccgttaag gctgtctgaa aagaagcttt gcccgtttcg
    11581 ctgaaaatct tttcagacgg tctttacgcc atcctcaacc tgactccctc ccctgcgggg
    11641 gagggttggg gagagggtgc agacagcagc ccttaagcaa acgctgcctg aaaaacgcaa
    11701 gctcgaccct ctccctaacc ctctcccatg ggagagggaa caaatcacag caccatcgac
    11761 attttcagac ggcatcggta aagaaaccca tctatgaacg cgttcaacct caccatcatc
    11821 ggcgacgaaa tcttacacgg cagccgccaa gacaagcatt tcgccttttt caaatccctg
    11881 ctcgaatcca aagggctgaa gctcaatcag gtgcaatatc tgcccgacga acccgatttg
    11941 ctggtcaggc aactgcgccg cagcttttca gacggcatac cgacgtttgt taccggcggc
    12001 atcggcgcca cgcccgacga ccacacgcgc caagccgcag cggctgcttt ggatttgcct
    12061 gtcgtccgcc atcctgaagc cgccaagttt atcgaaggca taacccagaa acgcggtgag
    12121 ccgctcgatt cgcccgaaca cgcccaacgc ctgaagatgg cggattttcc ccaaggcgcg
    12181 gaattggtgc ctaatccgtt taataatatc gccggatttt ccattcgcga gcattatttc
    12241 ttccctggtt ttcctgtgat ggcgcacccg atggcggaat gggttttgga tacttattac
    12301 gtcggccgct tcaaccaaac caaacgcggc agccgcagcg tgtatgtgtt tgagcagccc
    12361 gaatcgcgca ttacgccgct tatggagcat atcgagcaaa cttatcccgg tgtgcgttcg
    12421 tacagcctgc ccagcgtcgg ttggacgcat tcagacggca cgcaggtcaa accgcacatt
    12481 gagttcggca tcaaagccga aggagaagcc gtcaaccttt tggacgcggc gtgggcggaa
    12541 gtgttgcaca gcttggacgg attgggagca gagctgaaaa atcgggtaaa ctgaaaaccg
    12601 tttgcccaaa tcggacgata tttaaggaaa accatgcaat accgaatcag aagagaaaac
    12661 gaagcacccg aagcaaaaaa tacgggggaa accttatggg agcgcgacat catgcgcgaa
    12721 gtgctgctgt ccgcctatca ggacaggcgc agggagcgga tgtggaaaaa catctggcgt
    12781 gccgtcagca ccctgattct ggttgccctg attgcaggca ttttccgaaa agacgaagcc
    12841 gcattgcagt tggcgggcaa cacgccgcat accgcagtcg tcaacctgta cggcgaaatc
    12901 ggcaacggtg tagaagacca ggtcaaaaaa ctcaaagacg gtatggaggc cgcctacaaa
    12961 aacccgcagg caaaagccat cgtcatccgc gccaacagcc ccggcggttc gcccgtcgtc
    13021 tccaacaccg cttttgaaga aatacgccgt ctgaaggcgc agcaccccgg cattcccgtt
    13081 tatctcgtgg cggaagatat gtgcgcgtcc ggctgctact acatcgcggc ggcggcggac
    13141 aaaatctatg ccgacccgtc cagcatcgtc ggcagcatcg gcgttatcgg cagcagtttc
    13201 gacgcgaccg gcctgatgga aaaaatcggc gtgaaacgca gggttaaaat tgcaggcagc
    13261 aacaaaggca tgggcgatcc gttttcgccc gaaacgcccg aacagtcgaa aatctgggaa
    13321 gaaatgctga ccggcataca cggcgaattc atcaaagccg tcaaaaccgg acggggcggc
    13381 cgcctcaaat tccgacaata tcccgacgtg ttcagcggtc gcgtgtacac aggcgcggat
    13441 gcgctgaaag tcgggctggt ggacggactc ggcaatattt acagcgtggc gcgagacgtg
    13501 gtcaaagccc ccgatgtggt ggattacact ccgaaggacg atttcggcag aatcttgggc
    13561 agacgcttcg gcgcagaact caaagcaagc gtgcgcgaag ccctgcaggc cgtccggtag
    13621 cgttgtttac agtatttcta agggaatacc aaaataaaaa tatacacaaa aacactcacc
    13681 tccccagtat gctgccacta agtcagccgc gtcactattt accgtgcact taaagccgta
    13741 aaaggcaggt tgtccccggg aggttttgtg catcctgata ccgatcggtt gttgttgctc
    13801 aaaggacaga aggccgctga taaacgagat tacctgtttg tcgctattga cgatttttat
    13861 actctgccat tttgccagac aaaaccgcag acagtgctgc caagtttctg accgaacatc
    13921 tggccgaccc ctgcttgtac ctgattgagt acgtttactc tgacaatgat aggtaatata
    13981 aagagccgtc caacatgctt tcggtgcagc ttgttataat aatgggattg gttggaggct
    14041 tgcccgattt gcttgtccgc agaccaacgg taaggcggag cgggttatcc gtaccttgat
    14101 ggagatgtgg catgaggaac agtcgtttga cagtccggaa catcggcaaa aggagttgtg
    14161 ctgctttatt aatttagggg ctaccctaga tcactaggga aattcaaatt aagttagaat
    14221 tatccctatg agagaaagtc gtctaagccg gtataaacaa aataaactca ttttgttatc
    14281 gtggctatga tgtattggat gcgggcgaat ttagccattt ccgtatcaat cacagcacac
    14341 attttgccga acgacaaaac catattaatg gaactgagaa cttttggaat caagcaaaac
    14401 gtcatttacg caagtttaac ggcattccca aagcgcattt tgagctgtat ttaaaggagt
    14461 gcgaacgacg ttttaacaac agtgagataa aagttcttgt tccattttaa aacaattagt
    14521 aaaatcaagt ttgtcctagt tgtccagggc agcctcatct ctttatagat agctgcttac
    14581 caagtatcct taattgctaa ttcaagccac tcttcaaaac tattaaaaaa ttcatggttt
    14641 tctatattat ctaaattaat gacaataacc ctaggatttc ctgttacatc attaccatca
    14701 aaataagctt cccattctcc attactagcg aatgggataa gatttaaatc aggcaggata
    14761 gaatttctaa atatataagt tacttctgcc ccttctgttc cataattctc aaaccaccag
    14821 tgaaaattat gtggctgaat ataaggtatt aaagatgtat cgttagagat acgaacatac
    14881 ttttcaggat aacaaaacct ttcaggaagc atattactag gatataaatt aaattcattt
    14941 tgaataatca tttcgttacc ttaggatcat aaatactacc attaggaaac tgggtagacc
    15001 aatttggtgg tgctagttca ttttcaattt gttttttaat tctaaattga atagcttctc
    15061 cccatgtttg cggcatctaa tgctgcttgc tctgcatcaa tattactaaa aattggatta
    15121 gtctcaaaaa tgaaaaatta ctgttttttc attttttata gcatattcaa gtacatgaat
    15181 aatatttttc actaagggct ctgtagaaaa atctgaattt tgtaattttt cttttacagc
    15241 caaaatactt tccttatcta ttagccaatc cccctcactt ggtccaaata accaattatc
    15301 ggtaatttca gaaacaagtc tccataatcc acgattttga tattcaataa tttgattttc
    15361 cgtaatttct acaactgttt cagattgaat gtttttataa gcccaatcaa tcatttcagt
    15421 atattcacta attgaaacat tgtcttttaa acagaaaatt aacaggttgt gttcattcat
    15481 ttgtctaagt tcctgatatg agtttgttgt tgtaaataat cttttgcttg cttaccttgt
    15541 aaattaaagg attaaccgca ccttggatga tgccgcgaat atcatccata cggttagccc
    15601 accaattttt attggggtcg ttttcccata tcagttttcc tgcttcatca gcacaattag
    15661 ggttggcatt gatgcccacc cagttccagc ctgaaaagga tttttaatat acggcaaaag
    15721 gtgggcaaca agtgcccacc ttacctagct aacatttatc ccaagtatca tatatttttt
    15781 taagttcttt atccaaagaa agaactttat tttttcaaca ccatgaatta tctcaaaatc
    15841 tctacaatcc acgcatacgc ctgaataaat ttctcctaca attgcttgaa taatatacaa
    15901 ctcatcttta tcgagactaa ttttaactga actctcttca aaattaatta atttcatgaa
    15961 taattccttt tattttaatt cgtcatcaaa ataccgtttg cctgtttttg gtctaaatgc
    16021 tgtccaccat ctttagtctt ggatatttag attataatcg tcaaattccc agtcaaaata
    16081 ttttgccaaa tttgatatag acttatcttc agacgttgaa ttacatattt ccaatatatc
    16141 atttatcaat tttaatatgt gttcaatatt aaaacggttt gtaacaattg agttttttaa
    16201 tgacatcggt ctatgtatat ttgaaagtaa ccattcggtg ctatatatat aaacataaaa
    16261 ataatcagca ccattacttt catcaaaacc tattgataat gtcaaaagta tacaaaaatt
    16321 ttctaacttg tcttgtggga aatcttccaa atcaaagtca gaacaatcat aattttttaa
    16381 ttttaatctc ataatatatt gagatagctt aggaatttta ttttctcaga ttgaggcaac
    16441 tcatcaaaac caagccaaca ataaaaattg ttatatgatt ttaaaaaatc aacaattttt
    16501 atttttccta aaaccaaatc ttcgactaac tgggttgcat aaataatatc atttatgatt
    16561 ttaacaggta aattatttga taggtaacca tgggattccg tcatcattga tttctctagg
    16621 tgcttattta tctgattggt aagtcttttg cgtaagcttc tttcgcctcc gcaagtgtca
    16681 tttcattagg gccaccatct atattagttt ctcctgtatt ttgccactga caaacgtcac
    16741 agatatcata gtccataact tcagttccgc aaacaggaca atgtagccat atttctccgt
    16801 caatttccca tgtctttttt gaattctcca tcataatact tctttcctaa gtttggttta
    16861 aacattgttt tgattttgaa tgtttttgga ttggccaaaa catagatatt gttattgata
    16921 tcatagcgca ctcgtctatg ttccgtctga taacctaata tcttgtccga tgtctgtttt
    16981 gataacaaat cagacgccat tttttggtag tcttcaatgg ttatatcgcc aaattctttt
    17041 ccatgagctt tgaaatgtcc atttaaagat ttctcagtag gaaacttgga ctttgtccat
    17101 ctgatgcggt cttgaattgt tcctgtcgga gaaatagatg tatcaggttt aacatatgag
    17161 gcaggtaagc cgatattgtt atcagagtgt gtagggtgtt gctgcgtact tggttttggt
    17221 ggcttattcc cacctgccat ctccccatct aaagtctgca tagggcgggc agtaggtgtt
    17281 ttatagccgg tatttttaac ccaatcccat ttggtcgggt taagttctac ttttttaccg
    17341 ccccaaaccg tacctccagt caggtgtagt tggatgggga ttcattttta ataatatctt
    17401 caagcaagtg aaatgtaaga aaagcgtaga tacttgaagg ccggctatct gattggcaaa
    17461 aagaaatttc ggagataaag aatttccgtt tatatttttc ataaattaaa tcaacacctt
    17521 gttttacata aaaactaact tcctcaggaa ggatatttat aacattctct ccttttactt
    17581 ttatagttaa tggtttgatt tttatgtttt catgaatatc tgataggatt atttctaaaa
    17641 aattattttt atttccagtc attctggcta ttttatgtat atttccattg tgttcgatta
    17701 tcattttatt ttttccaagg atttccttct atattgatag tttatctaaa gatttcattt
    17761 aaaatatcct gactaatttc ttgtttatat aattctacaa ttttctgttt ctgtgtattt
    17821 gtataaaatt tttcgttggt atctgcttga tattttaatt gtctcaatat ttccatgatt
    17881 tcatattctg atttttcatc atattcttca atcatgtaat caacaaattg ttttagaata
    17941 tcaaaggctt cgtaactttc caaaatttta atatgtttat cggcaaaatt ggacatatac
    18001 cattcgtcag tgattggatt agtatttaaa tcatctaaaa aaacatttac atcatctaaa
    18061 aacatgacta atccttaaac ttaaaatctg ttttctcacc ggttcttgtg ttttcaataa
    18121 acttaaaatc tgttttttca ccggttcttg tgttttcaat gtaatgtatt tcaattttgt
    18181 ttacgttttg aacacgctta acccaaccgt cttttgctaa ccaaccattt ttagtatcgg
    18241 acataggagg tattctcgca ggagttttgc cctgtgggtt tgccttaact tcttccattg
    18301 ctaattgttc ctttaaatta ttaggtattg tacgaccggt tgatttttca acagattgtg
    18361 tccctatttt taatgatttg tttcccccag ccatttcccc gtctatagtc tgcata
//
LOCUS       DAWFIE010000037        18405 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWFIE010000037 DAWFIE010000000
VERSION     DAWFIE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18405
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.037"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(149..1081)
                     /gene="cysK"
                     /locus_tag="WGQ71_001170"
     CDS             complement(149..1081)
                     /gene="cysK"
                     /locus_tag="WGQ71_001170"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ0707183.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1275..1880)
                     /locus_tag="WGQ71_001171"
     CDS             complement(1275..1880)
                     /locus_tag="WGQ71_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707184.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1877..2728)
                     /gene="dapF"
                     /locus_tag="WGQ71_001172"
     CDS             complement(1877..2728)
                     /gene="dapF"
                     /locus_tag="WGQ71_001172"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ0707185.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAEAVF
                     EGELAYS"
     gene            2865..3161
                     /locus_tag="WGQ71_001173"
     CDS             2865..3161
                     /locus_tag="WGQ71_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707186.1"
                     /translation="MSRLFLLLFPCAILAACTRNISASPAVSDCAHLQNRTVLYFDAH
                     GKPADSAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3175..3843
                     /locus_tag="WGQ71_001174"
     CDS             3175..3843
                     /locus_tag="WGQ71_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0707187.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3916..6036)
                     /gene="pnp"
                     /locus_tag="WGQ71_001175"
     CDS             complement(3916..6036)
                     /gene="pnp"
                     /locus_tag="WGQ71_001175"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ0707188.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYINGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQAAINA
                     INEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWNA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RVGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6273..7136)
                     /locus_tag="WGQ71_001176"
     CDS             complement(6273..7136)
                     /locus_tag="WGQ71_001176"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ0707189.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKVLEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7334..8209
                     /gene="rfbD"
                     /locus_tag="WGQ71_001177"
     CDS             7334..8209
                     /gene="rfbD"
                     /locus_tag="WGQ71_001177"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ0707190.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYVGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQ
                     KALAQVVSKLLSSGY"
     gene            complement(8948..9181)
                     /locus_tag="WGQ71_001178"
     CDS             complement(8948..9181)
                     /locus_tag="WGQ71_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ0707191.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(9347..9547)
                     /locus_tag="WGQ71_001179"
     CDS             complement(9347..9547)
                     /locus_tag="WGQ71_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ0707192.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(9719..10594)
                     /gene="xerD"
                     /locus_tag="WGQ71_001180"
     CDS             complement(9719..10594)
                     /gene="xerD"
                     /locus_tag="WGQ71_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ0707193.1"
                     /translation="MEEGLIDRLLETLWLDRRLNQNTLNGYRRDLEKIARRLSQSGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREDIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVQHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(10646..11086)
                     /locus_tag="WGQ71_001181"
     CDS             complement(10646..11086)
                     /locus_tag="WGQ71_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ0707194.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            11407..12345
                     /locus_tag="WGQ71_001182"
     CDS             11407..12345
                     /locus_tag="WGQ71_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ0707195.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRLKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            12474..12767
                     /gene="hfq"
                     /locus_tag="WGQ71_001183"
     CDS             12474..12767
                     /gene="hfq"
                     /locus_tag="WGQ71_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ0707196.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            12852..13418
                     /locus_tag="WGQ71_001184"
     CDS             12852..13418
                     /locus_tag="WGQ71_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0707197.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALNNTRCRLQEAGYSNVRFILDGHENLKQYIPKPLDAAIFNFGWLP
                     GGDKSLTTRTETSIAALSATLSLLKENGMLIAVLYPGHENGKQEAEAVGQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            13472..13846
                     /locus_tag="WGQ71_001185"
     CDS             13472..13846
                     /locus_tag="WGQ71_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ0707198.1"
                     /translation="MQYLFVKYSHQIFVTITILVFNIRFFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCLACIVYLAKTKVLPF"
     gene            13856..14350
                     /gene="folK"
                     /locus_tag="WGQ71_001186"
     CDS             13856..14350
                     /gene="folK"
                     /locus_tag="WGQ71_001186"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ0707199.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKHGKVAELSKRLGNQGIRL
                     LPDK"
     gene            complement(14477..14956)
                     /locus_tag="WGQ71_001187"
     CDS             complement(14477..14956)
                     /locus_tag="WGQ71_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ0707200.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVFQI"
     gene            complement(15176..15913)
                     /gene="ubiE"
                     /locus_tag="WGQ71_001188"
     CDS             complement(15176..15913)
                     /gene="ubiE"
                     /locus_tag="WGQ71_001188"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ0707201.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDTALKEMCRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(15956..16324)
                     /locus_tag="WGQ71_001189"
     CDS             complement(15956..16324)
                     /locus_tag="WGQ71_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ0707202.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKRSDC"
     gene            16444..18063
                     /gene="mnmC"
                     /locus_tag="WGQ71_001190"
     CDS             16444..18063
                     /gene="mnmC"
                     /locus_tag="WGQ71_001190"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ0707203.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHNTEQTELLLTGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRALLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFSATHIIYCTGAHSSC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAQLSPALAESLFAANPIPQKHQGHAAMRCDSPDHLPLVGAL
                     GDIAAMQQTYAKLALDKNYRIDAPCPYLPNAYANTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
BASE COUNT         4695 a         5451 c         3945 g         4314 t
ORIGIN      
        1 atatttccct caagcaatgc ggcggcacca atacctgatt aaaacacaac cggtgttttc
       61 ggcatatatg caaaaacgca ttttccggca cggcgttaca ccgataccga aaaatgcgtc
      121 tgaatgtttc gacaatccga ttaaagcatt atgccaaatc tgcaaaaagt ggcgtagaga
      181 gatagcgttc gccataagaa ggcagcagca cgactatcag cttgccttcg ttttcaggct
      241 gtttggcaag ctgcaacgca ctccaaaccg ccgcaccgga agaaataccc accaaaatgc
      301 cttctttttc cgctattgcg cgggcggttt caaaagccgc ttcgttcgac actttggtaa
      361 tgctgtcgta aattttggta ttcaaaacgg tcggaataaa gcctgcgccg atgccttgaa
      421 tcgggtgcgg gcctttttcg ccgccgctca atacgggtga agcttcaggc tcgacggcaa
      481 ccactttaac ttcgggtttg tattttttca acacttcgcc cacgccggta atcgtaccgc
      541 ccgtgccgac gccggcaacg aagacatcga ctttgccgtc cgtatcccgc caaatttcct
      601 cggcggttgt tttgcggtgg acttcggggt ttgcctcatt gtcgaactgg cgcggcataa
      661 aataagtgtc gggatgcgcg tccaccaagg atttcgcttt ggcaatcgcg cccgccatac
      721 cttcggcggc aggggtcaga atcagctccg caccaaacgc gcgcaacagc attttgcgct
      781 ccttactcat gctttccggc atggtaatcg ccagcttgta gccgcgtgcg gcacatacca
      841 ttgccaaacc gacacccgta ttgccgctgg ttgcttcgac aatgacggtg tttttgttga
      901 ttttgcccgc tttttcggca ccctcgatca ttgcttcggc aatgcggtct ttgacgctgc
      961 tgcccggatt gaaaaattcc agtttcacgg caacctccgc cttcaaacct tcggtcagac
     1021 ggttcagttt gaccaaaggc gtgttgccga ttagttcggt gatgctgttt gcaattttca
     1081 tcttctgctc ctgaataata aggtttcgac agtaaggcgc aatccccata ggcatcctgt
     1141 ccgattgcag gggatttgcg ccgaaacata aacaaatttc ggatatggct ttatcctaaa
     1201 cctatgccgc ctaaaaaacc aatatccaaa atttctttct ttataacttt ttgttccgat
     1261 acggccgcac cgtattatct tccaatttct gaaagcagca gcaccagtac cgagacctgc
     1321 atcaacgctg ccgtcaaccc gccgagtcgc agctgcgagc cgccgaacgt tgacggcggc
     1381 tgccggtcta taccgcagcg gtggacatag gcaaccgcca tcagcacaaa ctgcaacacg
     1441 aaccaataaa ccggcttcag cgcataaagt ttgagcagtg ccgcaaacac aaacacttga
     1501 accgttcccg aaaagcaata atgcaccaac gcacatattc cgacaaaagc aagcgtcatc
     1561 gatacattac tgacggcaaa cagcccgagc agcggatgtt cggggtctgc ctgccatccg
     1621 tttccatccg ttttccggtt ccaatgcccg atgaaaaaaa atatgctgcc caaagtcagg
     1681 taaaaatggg ggatgaacaa gggcgcggcg cgggtcattc cccatatgcc gggcatcagc
     1741 tttgccccca aaaccgatat gcccagccac agcataatac tcgcccaaaa ccattgcaac
     1801 attcctgcgc ggaatatcag aaacagaaaa accgccgcat acagtaaggc agacaaaaaa
     1861 tccaaatgca gcaaaatcat gaatacgcca actcgccttc aaacaccgct tccgcagggc
     1921 cggtcatcat cacatcgccg ccgcaggccc attcgatata taaagtcccg cccggcaaaa
     1981 tcacctctac cgttttccct tcatccaaca accccagacg gatacccgcc accacagccg
     2041 cacacgcgcc cgtaccgcac gcttgggttt cgcccacgcc gcgctcgaac acgcgcaaac
     2101 gaatcgcggt tcggctgaca atctgcataa agccgacgtt gacgcgttcg ggaaactgcc
     2161 tgtgcggttc gataagcgaa ccggtttcgc gcaccggcgc gcattctacg tcatcgacca
     2221 caatcaccgc atgggggttg cccatattga cgcagctgac aggctgaatg ccggattcga
     2281 gatgcacccc gtaaatacag gcatcatccc cctcgcccga ttcggggaca aacggtattt
     2341 cagacggcat aaacttcggt ttgcccatat tgaccgtaac catgccgtta tcggacaatt
     2401 tcggaaaaat aacgccattt gccgtttcaa cacaaatttc tttcttatcg gtcaaaccct
     2461 tgtctgcaac aaaacgggca aaacaacgcg ctccgttgcc gcattgcccg acctcgctgc
     2521 cgtcggcatt gaaaatacgg taacggaaat ccacgccttc ggtttccgaa cgcccgacca
     2581 ccaaaagctg gtcgaagccc acgccccgga agcggtccgc ccatgccgca atcggcgcgt
     2641 cctcgggggt aaaatcctga ctgaccgcgt caatcaccat aaaatcgttg cccaaaccgt
     2701 gcattttagt aaatttcaac gtgttcatat tattctgaaa tcccgtattg tctgttgctt
     2761 ccgaattccg aaatccccgc accgaaagga aatgcttcca tctcccgccg cctgaggatt
     2821 aaaatgagcc attatactgt ttgttacccc ttacaagaaa cacaatgtcc cgcctgttcc
     2881 tgctgctttt cccttgtgca atccttgctg cctgcacccg aaacatatcg gcaagccccg
     2941 ccgtttcgga ttgcgcgcat cttcaaaacc gtaccgtcct gtattttgac gcacacggca
     3001 aacccgccga cagcgcagta aagggcggat tttacaaaat tatcgaaata cagggtgcaa
     3061 actgcatcct tattcaagac tttaacagca cgggcaatgc cctcggcgga tcctatgtgc
     3121 cggactgccg caaatcatcc cttttcccga aaaatatttg acttcaaact gcctatgaac
     3181 cacgacatca ctttcctcac cctgttcctg ctcggtttct tcggcggaac gcactgcatc
     3241 ggtatgtgcg gcggattaag cagcgcgttt gcgctccaac tccccccgca tatcaaccgc
     3301 ttttggctga tcctgctgct taacacagga cgggtaagca gctatacggc aatcggcctg
     3361 atactcggat taatcggaca ggtcggcgtt tcactcgacc aaacccgcgt cctgcagaat
     3421 attttataca cggccgccaa cctcctgctg ctctttttag gcttatactt gagcggtatt
     3481 tcttccttgg cggcaaaaat cgagaaaatc ggcaaaccga tatggcggaa cctgaacccg
     3541 atactcaacc ggctgttacc cataaaatcc atacccgcct gccttgcggt cggaatatta
     3601 tggggttggc tgccgtgcgg actggtttac agcgcgtcgc tttacgcgct gggaagcggt
     3661 agtgcggcaa cgggcgggtt atatatgctt gcctttgcac tgggtacgct gcccaatctt
     3721 ttagcaatcg gcattttttc cctgcaactg aaaaaaatca tgcaaaaccg atatatccgc
     3781 ctgtgtacgg gattatccgt atcattatgg gcattatgga aacttgccgt cctgtggctg
     3841 taagccccat tcattcccga tacccgtaaa aaataccgtc tgaaacctgt tcagacggca
     3901 ctttcaccct aagcgttact cggcggcatt ttcctcacgg gcaggcgcgt ccagcagggc
     3961 tttgatggac aggcgcacac ggcctctgtc atccacttcc aatgctttca cgttcaccac
     4021 ctgaccgact tgcaggtagt caccgacatt gcgtacgcgc tcgtgggcga tttggctgat
     4081 gtgtaccaaa ccgtctttgc ccggcatcac gctgacaatc gcgccgacgt tgttgtcgag
     4141 gattttcacc acagtgcctt catacacttt gcccacttcc acttcggcag taatctgctc
     4201 gatacgtttt ttcgccgcat cgccggcttc ttgagtggtc gcagcaatgg taatcgtacc
     4261 gtcttcggca atattgattt ccgtaccggt ttcagcggta atcgaacgga tggtttcgcc
     4321 gcccttaccg ataacttcgc ggattttgtc ttggttgatt ttcatcgtaa acaaacgcgg
     4381 cgcgtgtgcg gacaactctt gcgggcccgc aacggcggct ttcatctgat ccaagatgtg
     4441 cagacgcgct tctttggcct gtgccaaagc gatttgcata atttctttgg taatgccttg
     4501 gattttgatg tccatttgca gcgcggtaac gccttcggtc gtaccggcca ctttaaagtc
     4561 catatcgccc aagtggtctt cgtcgcccaa aatgtcggtc aggacggcaa atttgttgcc
     4621 ttccagaatc agacccatcg cgataccggc aacgtgtgct ttcaaaggca cgccggcaga
     4681 cagcaggctc aggcagccgc cgcagacgga agccatagag gaagagccgt tggattcggt
     4741 aatttcggag accacgcgca tggtgtagct gaaatcttca ggtttcggca atacggccaa
     4801 caatgcacgt ttagccaaac ggccgtgacc gatttcacgg cgtttcggcg cacctacgcg
     4861 acccacttct ccggtagagt acggcggaaa gttgtagtgc agcatgaagc ggtcggtgta
     4921 ttcgccggac agcgcgtcaa tgatttgctc gtcgcgcgaa gtacccaaag ttgcaaccgc
     4981 caaagcttgg gtttcgccac gggtaaacaa tgcggaaccg tgtgtacgcg gcaatacgcc
     5041 ggtttggatg ttcaaaggac ggacggtgcg ggtgtcgcgg ccgtcgatgc gcggttgacc
     5101 gtccaaaatt tggctgcgga caacatcggc ttccaagtgt ttgaaaatac ctttgatttc
     5161 gttggcggcc aaagtatcgg tttcttcggt aatcaaggct tcttttaccg cattccaagc
     5221 ctcgtccaat ttggcggaac gcgcttgttt ttgacggatt ttgaacgctt ctttaatggt
     5281 ttcgccggca atcccgcgga ctttggcaac taattcttca ttggtttcag gtgctttcca
     5341 atcccaaact tccggattga cttcgtcggc aaattcattg atggcgttaa tcgccgcctg
     5401 catttgatcg tggccgtaaa ccaccgcgcc caacatcacg tcttcgggca ggattttggc
     5461 ttcggattcc accatcaaca cggctttgga cgtaccggcg accaccaagt ccaattgcga
     5521 tttagccaat tcggctttag tcgggttcaa aacgtacacg ccgtttatat aacccacgcg
     5581 tgccgcacca atcggaccgg caaacggtac gccgctcaac accagcgcgg cagacgcgcc
     5641 caacattgca ggaatgtcgg aatcgatttc gggatcgacg gacacgacca tcgctacgat
     5701 ttggatgtcg tggtagaaac cttcggggaa cagcggacga atcgggcggt cgatcagacg
     5761 gctggtcagg atttcttttt cgctttgttt gccttcgcgt ttgaagaaac cgccggggat
     5821 tttacctgcg gcgtaagtgc gttccaaata atcgacggtc agggggaaga agtcttgacc
     5881 ttctttcact tctttgttgg tggtaacggc aaccaaaaca acggtgtcgc ccatagagac
     5941 tttaacggca gcggcggctt ggcgggcaat ttcgccggtt tccaaagtaa cggtgtggtt
     6001 gccgtattgg aaagttttaa cgtgtttgtc gaacattatt gttcctttca aaataccgca
     6061 ctgctaaaac actaataatg cacactaaaa tccgaatgtg catagttagg gtttcagacc
     6121 gtgcggcagg ttgtaaacaa gctttcagac gacatttcgg acgctgaaag caattgcgga
     6181 ttataaaggc aaccatcctt aaaatccagt attgatacaa taaaaaggcc gtctgaaaca
     6241 tatttctttc agacggcctt caaacttaaa aatcaatcct gagtcagcaa agtcaatgct
     6301 tcccgatact tctcgacagt tttctgaatc acatcggcag gcactttcgg cgcaggggct
     6361 tttttgttcc aaccgctttg ttccagccag tcgcggacaa attgtttgtc aaaagacggc
     6421 ggatttgtgc cgactttata ttggtcggca ggccagaagc ggctcgaatc gggggtcaat
     6481 acctcatcca tcagcgtcag cgtaccgttt gtatccaaac cgaattcaaa tttggtatcg
     6541 caaataataa taccgcgcga tttggcatat tccgccgctt cggtgtaaag ccgaaccgcc
     6601 ttggcgcgca cttcttccgc caattctttg ccgataatgc gtccgcattc ttcaaagctg
     6661 atgttttcat cgtgatcgcc gactgcggct ttggtcgatg gcgtaaaaat cacttcaggc
     6721 agttgttgcg cttcctgcat accttcgggc agccgaatac cgcaaaccga gccggttttt
     6781 tgataatctt tccaaccgct gcctgccaga taaccgcgca caatcgcctc tactttcacc
     6841 ggagtgagct ttttagccac gacggcgcgt ttttctaaaa ctttggcttc gttttcaggc
     6901 aaaacatcgt aaaccgtttg accggtaaag tggttgggca taatatgcgc cagtttttta
     6961 aaccaaaaat tggaaatctg cgtcagaatc tcccctttgc tcggaatcgg gtcgtccaaa
     7021 atcacatcaa acgcggacag gcggtcggaa gcgaccatca gcatacgttt atcgtcgatt
     7081 tcatataaat cgcgcacttt tccagaatag atctttacca aaccaatctc actcatttcg
     7141 cccccctgaa aatatcttga aaataccgac ccgacacccg acaggtttga atcgcaaacc
     7201 gatattctag ccgaagtcgg cgcaaaacaa tacccatggc acaaaaagcc aacccgtcaa
     7261 ccgtcggcaa agttttggca ctataatacc gacagcaagt cctacaatac acttttacca
     7321 aaggaaatac ctcatgagaa tcctattgac aggctcgaaa agccaactgg cacgctgcct
     7381 gcgcgaccgt cttccggaag actgggaaac cattgcgacg gattccgcat ccctagacat
     7441 taccgatgcc gatgccgtct gcaacatggt caaaagtttc caacccgacg ccattgtcaa
     7501 cacggctgcc tatactgccg tcgacaaagc ggaaggcgat gcggcagcgg catttgccgt
     7561 caatgcttcc gccgtttaca accttgcctt ggcagcacat cgcgcccatg cccgattcat
     7621 ccacatctca accgactata tctttgacgg aaaagggaaa agaccctacc aggaaagcga
     7681 ctttaccaac ccttccaatg tatacggaca atccaaaacc gccggcgaac tgctcgcact
     7741 gtctgccaat cccgacagcc ttatcctgcg gacttcttgg ctgtttagcg aatacgggga
     7801 caactttgtc cgcaccatgc tgaaccttgc gcgggaacgt tccccgctgt ccgccgtcca
     7861 caaccaaatc ggctgcccga cctatgccgg cgacttgtcc gccgccatca tccgcctgtt
     7921 gcagcagtcc aatcccgttc gcggcattta ccactacgtc ggcggcaaat ccgtatcctg
     7981 gtacgaattt gcccaacata ttttccaagc ggcatcgcaa cagcagacat ccttccccgt
     8041 tcccgaattg actgccgttt cagacaagga atatccgacc gccgccccca ggcccgcata
     8101 cagcattttg gactgccgca aaatcgaaaa cgacttcagc atcaaaccgt cagactggca
     8161 aaaagccctt gcacaggtcg tttccaagct gctctctagt gggtactagg tataatgcag
     8221 gcatccgata aagcaaagcc cccgaagctg caacttcggg ggcttgtcgg atagaaagga
     8281 ggcttgaaga aatggcgaaa gtcattttat tcctgtttct tttgttagtt tgctctccag
     8341 cctactaatg aaaaactgaa ataacaaggg gtggcaaatt accgcgcccc tttcgccaac
     8401 ctttcttcga gccattttac tacaatggct ttaaaaatca aggattgcct gatgctgccc
     8461 gcccctccgt ttccgccgcc aagcaccgcc ttggcggttt tcttatatag tggattaaca
     8521 aaaaccagta cggcgttgcc tcgccttgcc gtactatttg tactgtctgc ggcttcgtcg
     8581 ccttgtcctg atttttgtta atccactata aaatctaccg attacacaaa cacatatcat
     8641 ctttacacaa acacatatca tctttacaca atcatgcttc catcaacagt aaaacatgat
     8701 atgattgcca acaataacat ctcacaataa attttctaat ttttattgaa aaaatcaata
     8761 aataagaatt ctcccaaccg acaaataaaa taaagaaagg gttaatatgc aacatcgaag
     8821 aagactagca atttaccaag catccaaacg tgcttccttt accggcaggt catccgcccc
     8881 gcaaaaacgt aaagaacgtt aatttgaaaa aaatgccgtc tgaagtcctg cttcagacgg
     8941 cattttttta ccgttcgaga aactgtttca acctgtcctc atccaaaaac cagtgacaga
     9001 tttcctcatc tcccgccaac aaaacgggaa ccagctcatt gtatttttct tccaaaacag
     9061 gattttcatc cacatcgacc acttccagcc cgaacccgta ttcattttga aaaggtttga
     9121 gtgcatcgcg cattttgtgg cacaagctgc aatattcacg aaacatcaag gtcaatttca
     9181 tccgcgtttc cttatctgtc aatttgcaca cgccaaagcc ttagacgcag cagaatcatg
     9241 gtctatttgg gaaaaaacaa tgttttcaag gaagatgata ctcaagtcct gccaaaaaca
     9301 gcaaaaatgc cgtctgaaca gttcagacgg catttccaaa accgttttac gcttgaacat
     9361 tgatacccgc cgtaaccgtc ggttgcgcat tgtgcgccgt cagaaacgaa gcggcaagct
     9421 ccccacagca gccgcagcag ctcgctacgc ccaattgcga ctgcaaatcg cccattgtgg
     9481 tcgcgccggc ggcgatggtt tccttgattt gatggtcggt aacggcattg cagatgcaga
     9541 caaacatttt gtgctccgtg tgttctcaaa ctatcgtacc gatagcggct tttattatcg
     9601 tatgcaaata taaataaaaa cggttcgcat tgcaaggtcg gcatacacgg tttatcttgg
     9661 taatttttta tcaagttttc atttccgatt gatttcttat taaaccgaag cagaagcctc
     9721 agtttcggga atggtgttcc ttcactacac cctgcaacca tacattggca acatgggtgt
     9781 aaatctgcgt cgtattcaaa tcggcatgtc ccaacatatc ctgaaccacg cgcaaatcca
     9841 atccgtgctg caccaagtgc gtggcaaagg cgtggcgcag gctgtgcggg ctgatgtgcc
     9901 cgatgcccgc cctacctgca tattctttga caatcatcca tgccaactga cgagaaatgc
     9961 ccgtcttttt ctgactgaca aacaatgcgt cgcaattcct gcctttcagc agaagtgggc
    10021 gtgcctccgt ataatagcgt tccacccaat acgccgactc ctgccccatc gggaccatcc
    10081 tctgcttatc gcccttgccc agcgcggtaa tacagcccct gtccaaatcc acattgccga
    10141 agttcagccc gaccgcctcg ctgacgcgca agccggtcgc gtacatcaat tcgagcagtg
    10201 ccttgtcccg caaaccgtgt ggcgtatcag tatccggagt ggcaagcagt cgggaaatct
    10261 gctgctcggt gatcagggtc ggaatattct tgtcgatttt gggcggtttc agcaaacggg
    10321 tgggattgtc cgtccttatg tcttcacgct ccatccatat atacaggcgt ttgcacgccg
    10381 ataatgcgcg cgcctgcgaa ctccgttgct ctccgtcaac ataaaccgcc gccgccaaat
    10441 ccgcttcgtc cgcatccttc agcgttctgc ccgattggga caggcggcgg gcgatttttt
    10501 ccaaatcgcg ccggtaaccg tttaaagtat tctgattgag ccgcctgtcc aaccacagcg
    10561 tttcaagcag cctgtcgatc aaaccttctt ccataccgtt ccaaacaaat gccgtctgaa
    10621 tcttcttcag acggcatggt tcacattatc gggaaagcgt ttccaatact tcctgcgcgt
    10681 gacccgccac tttgactttc cgccattcat gggcgatttc tccatcctta ttcaagacga
    10741 acgtactgcg ctcgatacct aacgactctt tcccgtacag tttcttcaat ttgatgacat
    10801 caaacaggcg gcacactgtt tcatccttgt cgctcaacag ctcgaaccgg aaaccctgct
    10861 tggcgcaaaa attctgatgc gcctttacgc cgtcgcggga aataccgacc acggtataac
    10921 ccaatgcctc aaactgttcc aaacgcgcat tgaaatccaa gccttccgtc gtacagccag
    10981 gcgtactgtc tttcggataa aaatacacga ccaaaggcag atgttctgcc gaatgaaaat
    11041 ccgcaccgct gctcgaaggc agggtaaatt catatttcac atccatagtc ctactcccga
    11101 tattcccatt attcaaaacg gcacgcagag gcaccgccgc aattgccaaa ccaaccccga
    11161 ttctaccgcc ccaaaggaca aggattcaac tgccggaaac atccaaaccg acacacgacg
    11221 gcatgaaaaa tatccatgtc aaaccacaaa atatgttccg atttaaaaac agaatgttat
    11281 aaaacccaat ccccaaaaca caacaagacc gcccgcctac gggcagtctc ctgtcagacg
    11341 acatacttta cagatggctg ttttttcaac aaaataacgc caatactcaa aaatatggaa
    11401 tcaaaaatgt ccatccatac tctgaaacgc ctgccctcat cgctgctgct cggtctctgc
    11461 ctttccctgc cgtcagccca cctttttgcc gacaacgaca ttttagggca atttttagaa
    11521 cagaacatgc ttacctcctc cgatccgata gaaatattcg ccgaaagcac aatacacccc
    11581 accaacaccc aagccattac aggcggtctg attctctcct cacagtctgc cctggtcgtc
    11641 aacaacaaaa ccggacagat actgtatcag aaaaacgccg acaggattat gcccatcgcc
    11701 tccatttcca aactgatgag cgcgatggtc gttttggatg caaacttgga catgaacgaa
    11761 accgttacca ttacgcccga cgaaatcgac cgcctcaaag ggaccggcag ccgtcttgcc
    11821 ataggtacgg cacttacacg caaagaactg ctgcacctga gcctgatgag cagcgaaaac
    11881 cgcgccaccc atgcattggg cagaacctac cccggcggca tgggcgcatt tgtcgccgcc
    11941 atgaaccgta aagcccaaag cctcggtatg tacggcagcc gcttttacga accgaccgga
    12001 ctcaacttcc aaaacgtttc taccgccaaa gacctgagcc ttatggtcaa cgccgccgcc
    12061 caatatccgc aaatccgcac caactcgact tccaactacg cctcggtaca gaccaaaaac
    12121 gggcagcaga actacaaaaa ctccaatgcg ctggtcaggg aaggcatgtg gaacatcgaa
    12181 ttgcagaaaa ccggctacat acgcgaagca ggcaggtcta tggttgtcaa agccaacatt
    12241 caaaatcaac ccgttaccat cgtattgctg aactcgccca catccgccac acgcgtcaac
    12301 gacgcccgca aaatcgaatc gtggatgctg cagcaacgct cctgacatac aaatgcccgg
    12361 cggaaaaccg cccaaacaca aaaaaaattg gtaatttttc ctattcaagg ctacaattcg
    12421 acacgcacag ggcatctgcc cgatttcaca atcataatag cggagttaaa aatatgacag
    12481 ctaaaggaca aatgttgcaa gatcccttcc tgaacgccct gcgtaaagag catgttccgg
    12541 tttcgattta cttagttaac ggtatcaaat tgcaaggtca ggttgagtct ttcgatcaat
    12601 acgttgttct cctgagaaac acttccgtca cccaaatggt ttacaaacac gccatttcca
    12661 ccatcgtacc ggcacgctcc gtcaacctac aacatgaaaa cagaccccaa gccgcaccga
    12721 cttcgaccct cgtccaagtg gaaaccgtcc agcagcctgc cgaataatcc gcacgaagca
    12781 tgacgtgtca tatctttcaa taccttaccg gacaacggta aggtattttt attttcagac
    12841 agcatttaaa aatgttattg caaaacatcc ttccattcgc ccattgcctt ttgcggaagg
    12901 cacttcccga aggcggcaat gctttggacg gcaccgccgg caacggacac gacacccttt
    12961 tcctcgcaca aaccgcaggc atccggggga aagtgtgggc attcgacatc cagccgcaag
    13021 ccctgaacaa cacccgatgc cgtctgcagg aagcaggtta cagcaatgta cggttcatct
    13081 tggacggaca tgaaaacctg aagcaatata ttccaaagcc gctggatgca gccattttca
    13141 atttcggctg gctgcccggc ggggacaaaa gccttaccac ccgcacggaa accagcattg
    13201 ccgccctttc tgccacctta tccctactga aagaaaacgg tatgcttatt gccgtcctct
    13261 atccgggaca cgaaaacggc aaacaggagg cagaagccgt cggacaatgg gcaaaaaacc
    13321 tgcctcaaga acagtttgcc gttttacgtt acggctttac caaccggaaa aacagcccac
    13381 cctatctgtt ggcatttgaa aaactgcgtc aaaaataact gtttgcggta aaataagccg
    13441 attccaaccc ttattttgat aagagccgac catgcagtat ctgtttgtca aatacagcca
    13501 ccaaatcttc gttaccatca ccattttggt attcaacatc cgttttttcc tactttggaa
    13561 aaatccagaa aagcccttgg cgggcttttg gaaggcactg ccacacctta acgacaccat
    13621 gctgctgttt acgggattgt ggctgatgaa aattacccat ttctccccgt tcaacgcgcc
    13681 ttggctcggt acaaaaatcc tgcttctgct cgcctatatc gcattgggta tgatgatgat
    13741 gcgcgcccgt ccgcgttcga ccaagttcta caccgtttac ctgctcgcca tgtgttgcct
    13801 cgcctgcatc gtttaccttg ccaaaaccaa agtcctgcct ttctgaaaca ccgttatgaa
    13861 caacagacat tttgccgtca tcgccctggg cagtaatctt gaaaaccctg cccaacaggt
    13921 acgcgccgca ttggacacgc tgtcgtccca tcctgacatc cgtcttaaac aggcttcctc
    13981 actgtatatg accgcgcccg tcggttacga caatcagccc gatttcgtca atgccgtctg
    14041 caccgtttcc accaccttgg acggcattgc cctgcttgcc gaactcaacc gtatcgaagc
    14101 cgatttcgga cgcgaacgca gcttccgcaa cgcgccgcgc acattggatt tggacattat
    14161 cgactttgac ggcatctcca gcgacgaccc ccgactcacc ctgccgcatc cgcgcgcgca
    14221 cgaacgcagt ttcgtcatac gccctttggc agaaatcctc cctgatttta ttttgggaaa
    14281 acacggaaag gttgccgaat tgtcaaaacg gctgggcaat caaggcatcc gtcttttacc
    14341 ggataagtaa ttccgcacgc ggatgccgtc tgaatgccgg tttcagacgg cattttttct
    14401 ttgccgccaa cacgcgtgca aaaaaatcgc cccttggaaa aggggcgcaa aaggaacaca
    14461 aaccactacc aaaactttaa atctgaaaca ctgcctgccg catactgtat ccgacaggat
    14521 ataaagccct cactaaatcg tttcgagaaa tccaaacttc ttcatcgccg acagaaaatc
    14581 tgcctttctc cggcaccagc tccaacaaaa acggttgaac cgccgtatgc agcctgtcct
    14641 tacaccgccc cagctttctg gacaacgcgg acaaggcacg cttatagaca ctatccttgc
    14701 ggtcaagttc ccgtgcactg ccgacccagc tcaaacgaag gttgcggctt tcatcctcaa
    14761 tcagcaaaat gcctgttttg acttccccta cctcgggctc gcatgaaagc gcaatataat
    14821 atttgccgtc gatgttgaca tacgccgctt catgcacctg acagttgcag ccggaagtgg
    14881 caagcagcga ataaacagaa tcccgatgca gtttcaaaat atcaagggaa atctgtttga
    14941 aaatactttc cggcattttt ccctcctttc gtctgctgcg ttgtttactg gattgactgc
    15001 attatacaga catctcccaa tcttgcaaat gataattgtt cctatttttt aaaaagctta
    15061 taaattattg atatatatgt aaattaattt ttaagattta aaacctttgt ctcaagaaac
    15121 acaaaaatgc cgtctgaaac gtgttcagac ggcattggct gcacaggcag tccgtttaaa
    15181 atttcacgcc cttatgcagc gcgacgatgc ccgcactcat attgtgataa tccacgctgt
    15241 caaagcctgc atccagcatc atctgtttca aagtttcctg atcggggtgc atacggatgg
    15301 attcggcaag atattgataa ctctccgcat ctttggcaat cagtttgccc atgaccggca
    15361 gcagcttgaa cgaataaaaa tcatacgcgc cttcgagtgg cttgtagatt ttggaaaact
    15421 ccaataccag caatgtcccg ccgggtttca aaacacggca catctctttc agcgcggtat
    15481 ccttgtgcgt catgttccgc aaaccgaacg ccacggaaac caagttgaaa taattgtcag
    15541 ggaaaggcag tttttccgca tcggcaagcg acactggcaa aatcatgcct tcgttcaaca
    15601 gacggtcgcg cccgacggtc agcatagagg aattaatatc ggtcagccaa acctcgcctt
    15661 ccttgccgac ccgtttcgcc caaccgcgcg acaaatcgcc cgtaccgccc gcaatgtcca
    15721 acactttatc gccttttttc aggtgcgccg tgttgatggt gaaatgcttc cacaccctgt
    15781 gcagccctgc cgacatcaca tcgttcataa tgtcgtagtt tttggcgacg gagtggaaca
    15841 cttccgccac ttttccggct ttctcatctt cgttgaccgt gctgaacccg aaatgcgttt
    15901 tctgtccgcc catattctaa ccacctccct gatgccgtcc tccggcatcc caaacttaac
    15961 aatctgatcg cttttcaaaa cgggaagcac ccgccgccgc caatttgtcc aaatattcct
    16021 gccacatcgc atcgtatccg tatgccagtc tatgcagata cgcccaatcg taaagaccgc
    16081 tgtcgtgccc gtccgaaaaa ctgattttca gcgcgtactg tccgacaggc tgcaaatccg
    16141 taatttgcac atccgccttg ccggtctgca aaacatcctg tcccacgccg tgtccgcgca
    16201 cttccgcact cggcgaatag acgcgtaaaa attcggcagg cagattttta ggttcgtccc
    16261 cataagtcaa aaccaacacg gtacggtttt tcaacaaacg gatttcaaca ggaatattat
    16321 tcaaaaaaca cctcggttcg gcatacaata tccggcaggc gcaggcgtgc cttcaaacgg
    16381 cgcgtatttt aacagcttta tcccgatacc gatattcaaa cccacacttt aaacggcatt
    16441 gatatggaca atctcgtatg ggacggcgtt cccgacatcc gcacactcga ccaaaccatc
    16501 cgcaaacacg cacacccgct caacctgatt gtctgcctcc ccgataatca gattcccgat
    16561 tttcaaaccg cacaagatgc ttcggacgcg gaatgccgtc tgaagcaccg tttggatcaa
    16621 gcaacgcagt gcctccagtt cgacagcatc aacctcatcg aacacatcct gcccgatgtc
    16681 cgcttctggc tggttccccc ttcacgcacc cgccgcctgc acgaacactt ccaccacatt
    16741 tcctggcaga ccgaagccat cccgcaaacc gaaagtaagc ccgacaaacc ctggtttgca
    16801 cttccacaaa catccgaacg gcaaaaaccg gaacacatcc tcgttatcgg cgcgggcata
    16861 tccggcgcgg caaccgccca cgccttagca tcatacggca tttccgttac cgtattggaa
    16921 gcccgaaaag ccgcccaagc cgccagcggc aaccgccaag ggctgctcta cgccaaaatc
    16981 tcgccacaca ataccgaaca aaccgaactt ttgctcaccg gctacggcta caccaaacgc
    17041 ctgctcggac acatcctgcc cgaatccgaa acctggggcg gcaacggcat catccacctc
    17101 aattacagcc gcaccgaaca acaacgcaat cacgaattgg gtttgcaaaa acaccataac
    17161 cacctctacc gcagcatcac gcaggcagaa gccgaaaaaa tcgccggtat cccactgtcc
    17221 gtcccatacg accacccttc atgcggactc tactggcaac acggcgtatg gctcaatcca
    17281 cccgcattcg tccgcgccct cctcaaccat ccgctcattg gactacacga agacacaccg
    17341 ttaaccgaca tttcccacga cggggaaaag tggattgcaa gcacgccaaa cggcatattt
    17401 tccgccacac acatcatcta ctgcaccggc gcgcacagct cctgcctgcc cgaaaccaac
    17461 ctcgccgccc tgcctctcag gcaaatacgc ggacaaaccg gcctcacacc atccaccccg
    17521 ttttccgaac aactgcgttg cgccgtttca ggcgaaagct acatcagccc gtcgtggcac
    17581 ggactgcact gctacggcgc gagttttatt cccaacagca gccataccgg atggaacgaa
    17641 gccgaagaag cctcaaaccg ccaagcattg gcacagctta gccccgccct cgccgaatca
    17701 ttgtttgccg ccaacccaat cccccaaaaa caccaagggc acgccgccat gcgctgcgac
    17761 agccccgacc accttcccct agtcggcgca ctcggcgaca ttgccgctat gcaacaaact
    17821 tacgccaaac tcgcgctgga caaaaactat cgcatcgacg ccccctgccc gtacctgccc
    17881 aatgcctacg ccaacaccgc ccacggcaca cgcgggcttg ccaccgcccc catctgcgcc
    17941 gccgccgttg cagccgaaat cctaggcttg ccccatcccc tctcaaaacg cctgcgccac
    18001 gccctacacc ccaaccgcac catcatccgc gccatcgtca gaaggaagga tctaacccct
    18061 taagcctttg ccgcatgccg tcccgaccac agaaaatcat attcaaataa acataccaat
    18121 cctgacggta aaatatcgaa aacacccata acgttttctt cccaaaataa ggagtaagga
    18181 atgcctaaca aattgccttt gctgtccaag atgaatattc cgtaacattc gaagacttcc
    18241 ttaaccctgc cagccgtttc ggtttctgta ccccctgtta gataaggaag gaatgaaaaa
    18301 gccctgttga ttgtatgaag taaagggcgt taatttttgt taatcttccc ttctcaggaa
    18361 ctcaatatat aaggatgtta atttttattt cccttctcga ggact
//
LOCUS       DAWFIE010000038        17751 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWFIE010000038 DAWFIE010000000
VERSION     DAWFIE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17751
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.038"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(84..890)
                     /locus_tag="WGQ71_001191"
     CDS             complement(84..890)
                     /locus_tag="WGQ71_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ0707204.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGLGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYSLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1111..2481
                     /gene="ffh"
                     /locus_tag="WGQ71_001192"
     CDS             1111..2481
                     /gene="ffh"
                     /locus_tag="WGQ71_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ0707205.1"
                     /translation="MLDNLTGRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
     gene            complement(2812..3507)
                     /locus_tag="WGQ71_001193"
     CDS             complement(2812..3507)
                     /locus_tag="WGQ71_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0707206.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSNTSAPATPAA
                     LTEGINYTVLSNPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(3678..4337)
                     /locus_tag="WGQ71_001194"
     CDS             complement(3678..4337)
                     /locus_tag="WGQ71_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ0707207.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRRNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            complement(4540..6651)
                     /locus_tag="WGQ71_001195"
                     /pseudo
     CDS             complement(4540..6651)
                     /locus_tag="WGQ71_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(6837..7442)
                     /locus_tag="WGQ71_001196"
     CDS             complement(6837..7442)
                     /locus_tag="WGQ71_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0707208.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAAVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(7491..7889)
                     /locus_tag="WGQ71_001197"
     CDS             complement(7491..7889)
                     /locus_tag="WGQ71_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0707209.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            7997..8908
                     /locus_tag="WGQ71_001198"
     CDS             7997..8908
                     /locus_tag="WGQ71_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0707210.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            8898..10199
                     /locus_tag="WGQ71_001199"
     CDS             8898..10199
                     /locus_tag="WGQ71_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ0707211.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQYADDDPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGGLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDVAINQGNWRLAASRHTYPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAYPVAGIP"
     gene            complement(10267..10491)
                     /locus_tag="WGQ71_001200"
     CDS             complement(10267..10491)
                     /locus_tag="WGQ71_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707212.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVSAWVAVIWAYLIIESEKNGRY"
     gene            10594..12744
                     /gene="dinG"
                     /locus_tag="WGQ71_001201"
     CDS             10594..12744
                     /gene="dinG"
                     /locus_tag="WGQ71_001201"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ0707213.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSLGVSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGEFGIFIVRIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            12781..13395
                     /locus_tag="WGQ71_001202"
     CDS             12781..13395
                     /locus_tag="WGQ71_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ0707214.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGIASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAKRRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
     gene            complement(13686..15554)
                     /locus_tag="WGQ71_001203"
     CDS             complement(13686..15554)
                     /locus_tag="WGQ71_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0707215.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVMIFHLNDRWLPGGFLGVDIFFVI
                     SGFLITGIILAEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHESLLKHSHGNALQ"
     gene            complement(15635..17005)
                     /gene="purB"
                     /locus_tag="WGQ71_001204"
     CDS             complement(15635..17005)
                     /gene="purB"
                     /locus_tag="WGQ71_001204"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ0707216.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIVEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFVHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGLLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(17077..17709)
                     /locus_tag="WGQ71_001205"
     CDS             complement(17077..17709)
                     /locus_tag="WGQ71_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ0707217.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
BASE COUNT         4321 a         4841 c         4579 g         4010 t
ORIGIN      
        1 ttcctctccc tcctccggct ggtgcgcctt tgtgaatatg ccgtctgaaa ctcggggact
       61 cagacggcat ctgttggctc ttcttatctt ttcagaatga tttccaatac gaacttgctg
      121 cccatatagg caatcataag gctgacaaat ccgatgatgg tccacacggc ggcttttttg
      181 ccgcgccatg cggtcatgct gtgcttgagc agcagtccgc cgtaaatcag ccatgacaat
      241 atgccgaata cggttttatg ggtaaaggtc atgggtttgc cgaatacggc ttcggcaaaa
      301 aatgttccgc tgacgacgga ataggtcagc aggatgaaac ctgcccacat ggcttggaac
      361 atgagttttt ccaaactgag cagcgacggc aggaatcctg cgagcttgga gaagttcctg
      421 cggtgcaggc tccgattcag cagcagggtc aaaacggaca ataatgttgc gatgccgaac
      481 agcccgtatg cgagcagcga agttccgata tgcagcataa agggaaggtc ggtaatttca
      541 tatcccgaga attttccagg aaaaaccaaa cctgacagca gcatcagtgc ggcgcaagga
      601 tacagcagca actgcactcc gcgcagcgaa taaaagaagc tgccggcaaa ataaataaac
      661 agcatcatcc aaacaatcag gctgccggaa tacccaaagc ccataatgat gattttgtct
      721 tgaatgaccg gcataagcag tgccgcgcca tggacggtca atgccgcgcc caaaaccggc
      781 aattccgtct tccacgggta atcccggccg agcccctgct gttggcagtg ccatgcaaat
      841 gcacccaatc ctgcgtaaac cgccgtcaaa aagatgaaaa ctgtcggcat ggtggacttt
      901 ctctaatcta tactgttgcg ctgtatgcgg ccgcttatga aatattggaa cttttaacgt
      961 tggaattgta aaatccccgt ttcgggcaag ccttgacgga tttgccgata tgctgtccgg
     1021 cacacaagcc gcatcaaatt attttgattt tattttaaca aagaatgccc ctgatggggc
     1081 aagctattct tattcagacc aaggaccagt atgttagaca atttaaccgg ccgcttcagc
     1141 aatgttctca aaaacatccg cgggcaggcc aaactgaccg aagacaatat taaagaagcc
     1201 ttgcgcgaag tccgcctcgc cctgcttgag gcggatgtcg ccctgcctgt cgtcaaagag
     1261 ttcgtcaaca acgtcaaaga aaaggccctc ggtcaggaag tagcgggcag cctgacgccg
     1321 gatcaggcat ttatcggcgt ggtcaacaaa gccctgaccg agctgatggg cagggaaaac
     1381 aaaacgctgg atttgtcggt tgcgccgccc gccgtcgtgt tgatggcagg tttgcagggc
     1441 gcgggcaaga cgacgaccgc cggcaaactc gcccgcctgc tgaaaaacga tcagaagaaa
     1501 aaggttttgg tcgtatctgc cgacgtttac cgccctgccg cgattgaaca actgcgtctg
     1561 ttggccgaac aggtcggcgt ggactttttc ccatccgata ccaaccaaaa accggttgag
     1621 attgcaactg ccgccgtcga ttacgccaaa aaacattttt acgatgtatt gatggtcgat
     1681 accgccggcc gtttggcaat cgatgaggag atgatgaacg aaatcaaagc ccttcacgcg
     1741 gcggttaacc cggtggaaac tttgttcgtc atcgatgcga tgctgggtca ggatgcggtg
     1801 aacactgctc aggcatttaa tgaagccctg ccgctgaccg gtgtcgtatt gaccaagatg
     1861 gacggcgatt cgcgcggcgg cgcagctttg tccgtgcgcc acgtaaccgg caagccgatt
     1921 aaatttatcg gtgtcggcga aaaaatcaac ggcctcgaac ctttccaccc cgaccgtctt
     1981 gccagccgta tcctcggcat gggcgacgta ttgaccctga ttgaagacgt ccaaaaaggt
     2041 attgatgaag aagccgccgc caaaatggcg aaaaagctac aaaaaggcaa aggcttcgac
     2101 ctcaacgact ttaaagaaca aatccagcaa atgcgcaata tgggcggttt ggaaaacctg
     2161 atgtcgaaaa tgccgggcga actgggtcaa atatcgaaac aaatccccga aggaacggct
     2221 gaaaaagcga tgggcaaagt ggaagccatc atcaactcga tgactccaaa agaacgcgcc
     2281 aaccctgccc tgctcaaagc cagccgcaaa cgccgtattg caatgggtgc gggcacgacc
     2341 gtacaggaag tgaacaaatt gctcaaacag tttgaacaaa tgcaacaaat gatgaagatg
     2401 ttcagcggca acggcttggg caaactgatg cgtatggcga aaggaatgaa gggaatgaaa
     2461 ggaatgttcc cgggtttgta agccgatttg accgaaaacg ccgtctaaaa tttcagacgg
     2521 cgtttttgtt ttatattctg atttatagtg gattaacaaa aatcaggaca aggcggcgag
     2581 ccgcagacag tacagatagt acggaaccga ttcacttggt gcttcagcac cttagagaat
     2641 cgttctcttt gagctaaggc gagccaacgc cgtactggtt tttgttaatc cgctatatat
     2701 tctgatttaa aaccataagg ctttaagcaa tcatctcttc tataaagcct aaatacaaaa
     2761 ggccgtctga aatcctattt tcagatggcc tttactcatt caatcctcaa cttattgcgg
     2821 ctttttctgc tcttcgcgta ctttatccac caactggtca atcgtggtca taccggactg
     2881 ccagtcttta aattcaactt ggtatttgcc gccgacgatc acagtcggtg tgccgctgat
     2941 ttggaattta ttggtcaact cttccatttg agccgcacgc gcttggcttt caggagcctc
     3001 aaatgcagcc aatacttttt tgccgtcaaa cgctgtttgc tcggacagcc attttttcag
     3061 ggtatcggta tcggccagat tgattttttg attaaccatc gcatcgaaaa tatggctgtt
     3121 ggctttatct gattcaccgg ccatttccac tgcggccgcc aaacgtgcca aaggtttcat
     3181 ttcatcaccc cacacgacat gctcccggcg catataggta tcgtctttaa acgttttgat
     3241 gtgctcgctc aagaccggct caagatgggc gcaatgcggg cagaagtagc cgaagaattc
     3301 caatacttcg attttgccgg cctgctgttg cggaatcgga ttggacaata cggtgtaatt
     3361 gataccttcg gtcaatgcgg caggggttgc cggagcggat gtgttgcttt gggcgctgtc
     3421 tgccggaacg ctggtttcag cctgtttgct acaagcggcc aatgccaaca gggtcaatga
     3481 agtcaaagct aaggttttca gtttcatagg tatctcttgt gtgtcagatt aatgtgcgga
     3541 ttttatggca ttttattgaa ggcgcgttcg atttgagtga aaaaatgtgt ttagttttat
     3601 ttcagccttt ataaaggccg tctgaaatat ggcttagata attagcctta ttttcagacg
     3661 gcctttgttc tagtaattta cggtttcgga cattttgtct tcaatccgtc gatgtgttcc
     3721 attaagtcag gtgggacatg attccacata ggatcaagaa taaaatcgat accttcccgt
     3781 ctggctaatt tggctgctgg gacaaaatcg gaatcaccag aaaacaagat gattttatcg
     3841 gcttgttttt ttaaggtaat agatgaaata tccaagccta tacgcatatc tacacctttt
     3901 tgtttaacat cgagcacgac atcgttttcc gtaatttgag ttgacgggtt tttcagaaga
     3961 tttttcatga cttggggttt taatatccat gctgtattgt ccgacaactt gcctaaacgt
     4021 aacgctaatt tacgcttctt cttcaattcg gtatgcagat tgcgacgaaa tatggcttcc
     4081 ggagtctttg aaaaatctat ggcacgatga gatacgggat tgtgcatttt cttttcaaac
     4141 ggcacgcagt cgtaaaagaa aattctatat aaatcatcca tttgatctgt gccgttgatt
     4201 ttatctttca gatgcagcat cgaatatcgg aaagccaaat cagcaattct ttttgcatcg
     4261 tgtggatttt gagacaggcc taatgaccgt acccgcttaa taaaaaatgc accgtcaatc
     4321 aaaatggcgg ttttcatatt gcttccccta tatttgtcaa agatataaaa aagcccttgg
     4381 gatcggcata cccgctatga tttaatgggt aatgcgtact gccaagggcg ctatttcttt
     4441 ttggattata ctgatgtaga taaagcctgt caaaaagttc gtgtgctgaa tttacggatt
     4501 atcaggattt tcagacggca ttctgttgca cgaacgggat tagaaactgt aattaacccg
     4561 cgccgtataa gtgcgcggcg cgccgggcat ggcatccgaa cgccaatatt tttgattgag
     4621 cagattggct gcggcaaagg taatgttaac atttttatgg ttccagccaa gcatggcatc
     4681 aactcgggca aagcctggaa gcgtagtcac ttctttattt cttgagttgt aaccgtagcg
     4741 tttgcctgta ccggttacgc cgatttcgcc gtagaggttt tcggtcgggg tataacggaa
     4801 aaacaggttg ccggtaacgt tgctggtgtt attcaaatgg atgcccactc ggtcgggatt
     4861 ttctttgtct tcaacgactt tcgcctgcat cacgcccaac gaaccgcgca gatagagttt
     4921 tttagggatg atttgcccga tggcggacaa ttccacgccg cgcgaacggt gtttgccgct
     4981 aaccgcataa atataagggt tgttttttgg atcggggcgg tagcggatat tgaagcgttc
     5041 gatttggtag gcggacaatg tggtgctgag gcggtcgtcc agccaactgc ttttaacgcc
     5101 ggtttcgtat tggcgggtgt actcggggtc ggcgttgaac acggcggaag acaacgtatc
     5161 gatactcaaa tagccgccgc gtccgccata aggcgcgaag cctttgttat acgaggcgta
     5221 gagcgtgtgg gcgggcgtta cgttccacac tgcgccgatg ttggggctga acgagtgtcc
     5281 gctgtattgg cggctgctgc cggtgagttt gttttcggaa ttaaaggtgt atttgtcgta
     5341 acggccgccg aggacgaatt tcaaatcggg cgtggcggag aagatgtttt gcacgaagat
     5401 gccgtaggaa tcggccttgt gtcggttttg agtgaggacg ggctgcaatc tgcccgaagc
     5461 cggccagctt gctcggtcgt atggatcgat ggaagcagta aaggcgcggc tgtaacctaa
     5521 ggtcgggttg cggtgttcgc ggctgtaatc catgcctacg gtcaggtggt tttcaaaacg
     5581 gccgatggtg tagtcgccgt ttagcgtgaa attggacgac agggttttgt tgtcggtctg
     5641 ctgccaggcg tagttgcgtt tgattcggcc gccgttttcg ctgcctgcat agaaatggtc
     5701 aaaatcctgc gccgccgtgc ggtgggcgag ctaccattgg gcacgccatt tgtcgttgaa
     5761 ggcgtattca aggtcggaac gccaaacttg cagcttgtct tgcacaaaat cgttcgggtg
     5821 ggcaaaaccc atgcggtaag gcaatccgaa gcggtcgtac acagacttgg ttgggctgcg
     5881 gtcgggcgtg cgctccacat tgtcgtaggt gtattgcccc gtccacttca agccgttgtc
     5941 gagtttgacg gtaatgctgg gcgaaaccat gacgtttttg ctgtctatgc cgctgcggaa
     6001 cgaattggcg cgcccgactt cgccggtgag gcggatggcg acgtttttgt tcagcacttc
     6061 gttgatgtcc atattcaggc tgcggtttgc ccacgaaccg taaaccgcac cgatgttgcg
     6121 gctttgtttg aagttggcgt atttgctgac catgttgatg acgccgccgc cgttggtgcg
     6181 gccgtaaagc acggaagacg ggcctttcag gatttccacg cgctcgatgt tggcagtact
     6241 gcggcgcact tgtccgcttt cgcgcacgcc gtcgcggtaa atatcggatg cgtcggcttg
     6301 aaaaccgcgc aggaaaatgc tttcaccgcg catatcgtag gcagcgtcga tgccggcatt
     6361 gccttcgagg atggaactca aatcgttcgt accgtaattt ttgtttttct ggatattgag
     6421 cgtatcgatg gtttgcggcg tttctttgat gagctgtccg ttgcgggtaa cggcggcttc
     6481 gtcgtagttg atgtagcctt tgagtacgct ggtgtcggac tgtccgacca cggaaacggt
     6541 aggtagcgtg gcggtgtaat gttcaccatt gtcctgcgta tcggcggcag caacagggaa
     6601 ggaagcaata atcagtgtgg gtaataaagc taaatgaaat gatattttca tttttatact
     6661 caatttaaca aaacaaccga attatattgc ctcacggagg aaatgagaat aatttctttt
     6721 aactatattg aacatgatat ttgtaaacaa aggtctcaga atgcggaaaa ctcgccgcct
     6781 gatactgaaa aatgccgtct gaacagggtt cagacggcat ttttttgacc gcgaaattat
     6841 gcgccgaaca ctttcaaacg ttctgcaact ggttcaaagg tcttttcacc tgccgcccct
     6901 tcaataccgc cgataaccat ttgcgcgcgc aacaaccagt tttcggggat attccacgct
     6961 ttggcaatcg ccgcatcggg caagggattg taatgttgca ggtttgcacc tacgccgacc
     7021 gcggcaagtg tcgtccaaac ggcatactgc accatcgcgt tcgcctggtc cgcccaaacg
     7081 ggaaagttgg cggcataagc agggaactgc tcctgcaaac ctttgacgac attttgatct
     7141 tcataaaaca aaatggttgc cgcacccgcc ttaaacaggt tcaatttttg cgcggtcggt
     7201 tcaaaactgt cggcaggcac ggcggcacgc agcgcgtctt cgacaaattg ccacacctta
     7261 tcatgctctt cgccaaacag cacgaccacg cgggcagatt gggaattgaa cgaagaaggt
     7321 gtgtgcaaaa cggcgtgttc gacgatttgg acaacttcat ctttgccgac gggcagattt
     7381 ttatttaacg aataaatgga acggcggctt tcggcagcct gttgcagaga ttgacgggtc
     7441 atttcttttc ctcaaaaaca ttcaacaaaa tacactttag gcagtccgat tcaggcttta
     7501 cgcttgaaaa acagcctgtc cagcgaccat gctccgccgc ccgccgccgc gatatagagg
     7561 aatacgaagc agaacagcac tgcggactcg ccgccgttgg caatcgggaa caaagcattt
     7621 ccggaagcgt gcgccataaa ataggcaacc gccatctggc cggacaaaac aaacgcggca
     7681 gggcgcgcaa acaggcccaa caccagcaaa atgccgccga caatttctaa aataccggca
     7741 agcagcaaca gcccgccggg cgaaccgctg cccatttcaa tggggaaggc gaagattttc
     7801 gacgtaccgt gcaacaaaaa caggtaagcg gttacgatac gcaaaacaga aagcaaaacc
     7861 ggttggatac ggttgcagca atcggacata aaagctcctt tgatatttgt cttcatttca
     7921 ggcggtagag tatagatgtt tccccttttc aaatatatac tgcttttatt gatacaactt
     7981 ttccacacaa gaaaatatgg acaccctgtt cagcctcaag gttttccgcc aagtcgtcca
     8041 aagcggcggc ttcacccgcg ccgccgacgc gctcggcatc tccaccgcaa tggcaagcaa
     8101 acacgtcagc cacttggaaa acaccgtcca agccaaactc ctgcaccgca acagccgcaa
     8161 cctcagcctg accgaagccg gggaagaata ctaccggcaa tgcagttacg cgctcgacac
     8221 gctcgacgat gccgcccaaa aagccgccgg ggggacggaa aaaccgcagg ggctgctgcg
     8281 cgtaacgatg ccgctgtggt ttgccggcag ccagatatgc aactggctgg cggaataccg
     8341 cgaacgttat cccgaagtaa cattagaact gattttggac aaccgccacg tcgatttgat
     8401 tgccgaaggc gtggatttgg cgttgcgcgt ttcccaaacc ctgtccccgt cgctgattgc
     8461 gcgcccactg gcggaaatcg aatttgccct gctcgcctcg cccgatttcc tgaggcgcaa
     8521 cggcgtgccg gaaacgccgg aagaagtggc ggggctgccc gccgtcctgc cgacctacac
     8581 caaccagcag aaactcgacc tcacccgcaa atcggacggc aaaaaatacc ggcttgaact
     8641 gacccccgtc atccgtaccg acaacacgct gatgatgcgc gaaatgatta aggcgggcgc
     8701 gtgcatcggt tatcagccgc tttgggcggc ggaacacgat ttgcgctgcg gcacgctggt
     8761 aaggctgctg cccggatacg ccgtcccgac cgaccggctg aatgccgttt atgcagacag
     8821 ggcgttctta agcgcgaaag tccgtagctt catcgatttt ctgaacgaaa aaatcgccag
     8881 caggaaaggc tgccgaaatg ccgtctgaaa ccgcccgccc cctttatgcg gacacgtgcg
     8941 ccgcacacac gctcgtctgg ttccgccaaa acctccgcat ccgcgacaac gccgccttat
     9001 gcgccgccgt tgccgaaggt ttgcccgtta tcggtatttg gattgacgat gccgaaacag
     9061 acaaccctcg ccgcgccgcg ttctaccgcc aatccgccgc cgaactcgcc caagggcttg
     9121 cagggcgcgg catcccgctc tacacggcgg catcgcctgc cgagctcgtc cggctcgccg
     9181 tccgcctcaa tatccgcgcc gtcatcgccg acgaatccca tacttttgcc gacaaactcg
     9241 ccgacaacgc cctttggcat gaattggaca aacacggcat cgcgttaacc ttcgtcaacg
     9301 accgtgccgt tttcggcaaa accgacctga tacccgacgg cggcacggca cacaccgatt
     9361 tcgaccgcta ccgcgaagta tggctcgacc gcttttccaa gcagcccccc gccggtccgg
     9421 acctattcgc ggcataccgc caacccttcc ccgaaaacct ttccgccccg ccgcctgccg
     9481 ccctttcaga cggcatcttc ctgccgcaaa acagcggcga aacggcggct tggcggcagt
     9541 ggcggcggtt tctcgaacag gcggattcct actccgtttt aaaggatttc ccctcgcgca
     9601 aacacacttc gctgatgggc gcgtatttga gtgccggctg catctcgccg cgcctgctcg
     9661 cgcgggaaag cctcgaacgc cgtctgaacg cgtgggcgga caacatcatc cgccgcgatt
     9721 ttttccttca acttgccttg cagtacgcgg atgacgaccc ttcagacggc aatccggaac
     9781 acaccctgcg cctgacgctt tggcagcagg gccagaccgg cattccgatt atcgatgccg
     9841 cgatgcgctg tttgcacaaa accggtggcc tccaccccgc cctgagacgc ttgagcgcgg
     9901 actttttctg ccacgtttta aacctccccc gccgcgaagg cgagatatgg tttgcccgac
     9961 agctgaccga tttcgatgta gcaatcaacc aaggcaactg gcggcttgcc gcctcacggc
    10021 acacctaccc cgacattgcc gccgcctcat accgtaccga cccggacggt acttttatca
    10081 aacggcacat tcccgaactc gcccacctgt ccgccgacac cgtccacacg ccttggcggt
    10141 ttgcctgttc ggtcgatacc cacggctatc ccgcctatcc tgtcgccggt attccctaag
    10201 cggtacggca acctaaaaca aatgccgtct gaacttccgt ttcagacggc attgcgtccg
    10261 gatgactcaa tatcttccgt ttttttcact ttcaataatc agatatgccc aaatcaccgc
    10321 cacccaagcc gacaccgttc ccatcgccgg caaaaaatgc tgccaatccg tcatcggcgg
    10381 cacagggcaa cggccgcatt cgatttccgc gccgcccaac aaccggtaca gcaacatatc
    10441 cgcaatccaa agcaacaacg ggtatttcaa acgctgcaac caaaaaacca accacgtccg
    10501 cacgattcac ctcacccccc attccgacaa cgatattgta ccgcttccgt gcaatgttaa
    10561 aataagccca cttctaccgc cgtacaaaac gccatgctca ccgatttaga aaaaaacgcc
    10621 atccgcgacc attaccaaaa catcggcaaa aacctgcccg gtttccgtcc gcgtgcttcg
    10681 cagcgggaaa tgattgcggc ggttgccaac gctttttcgc ggacgttggc gcgcgaagaa
    10741 ggcggcgagc cgcccaagcg cgaaggcgag agcatcgccg taatcgaagg accgaccggc
    10801 gtgggcaaat cgcttgccta cctgctggcg ggcggcatca tggcgcaaac acgcggcaag
    10861 cggctgattg tgagcagcgc gacggttgcc ttgcaggagc agttggtaga ccgcgacctg
    10921 ccgtttctgg tcgaaaaaag cggtttggaa ctgaccttcg cacttgccaa agggcgcggc
    10981 cgctatctct gcccctacaa actctatcaa ctgacgcaaa gcaatgccca gcaaaacctg
    11041 ctcggctttg aagcccccgc cgtcttgtgg gacagcaaac ccaagcccga agaattgaag
    11101 ctgctgcgcg acatcgccga cgaattttcc gcccgccgct ttaatggcga ccgcgacact
    11161 tggccggaaa aaatcgatga cgcgatttgg ctcaaagtga ccaacgaccg ccacggctgc
    11221 ctgaaaaccg cctgtcccaa ccgtccggaa tgcccttttt acctggcacg cgatgtcttg
    11281 gaaaccgtcg atgtcgtcgt tgccaaccac gatcttctgc ttgccgacat cagcatgggc
    11341 ggcggtgtaa ttctgcctgc gcccgaaaac agtttctatt gcatcgacga agcgcaccac
    11401 ctgcccaaaa aagccctcag ccgttttgcc gccgaacatt catggaatat tgccgtttgg
    11461 acgctggaaa aactgccgca gcttaccggc aaaattgccg cgctgaccga taaagccgaa
    11521 cttgccaacc ttgccgacga agccgccgca tccttgctcg acagcctgca cgaatggcag
    11581 ttccatttgg cggaagagcc gtctttaagt ttgggggtgt ctgaaaacga cagacgaacc
    11641 aacagcgaac cgacttggct gtgggaagac ggcaaaatcc ccgaaggcct cgaaaccacc
    11701 gtttccaata cggccattgc tgcgcgcagc ctgctcaaac acgttgtcgg gctgaacgat
    11761 gcgctttccg ccgcgcgccg cgaaaaagaa caggacggcg cgctcctcga ccgcctgacc
    11821 ggcgagttcg gcatcttcat cgtccgtatc gaacaaatca gcgcggtttg ggatttgctc
    11881 tccaccgtcc ccctcgaagg cgaagagccg ctggcaaaat ggataacccg ccgtgccgac
    11941 gacaaaaacg actacatttt caacgccagc cccatcagca gcgcatccca ccttgccaac
    12001 agcctgtggc ggcgtgcggc aggcgcggta ttgacttccg ccaccctgca atccttgggc
    12061 aacttcaacc tgatgctgcg ccaaaccggg ctgcaatggc tgcccgaaac caccaccctc
    12121 gccctcaaaa gcccctttga ctttgaaaaa cagggcgaac tctacatccc ccccatatac
    12181 gccagcccca aagaccccga agcccacacc gccgccatca tcgaatggct gcccaagctt
    12241 atttcgccca ccgaagccat cggcacgctc gtcctgtttt cctcgcgcaa acaaatgcag
    12301 gatgtcgccc tgcgcctgcc cggagactac ctgccgctct tgctcgtaca aggcgaatta
    12361 cccaaagccg tcctcctgca aaaacaccac cgggccatag aagaaggcaa agccagcatc
    12421 atcttcggac tcgacagttt cgccgaagga ctcgacctgc ccggcaccgc ctgcgtgcaa
    12481 gtcatcatcg ccaagctgcc gtttgcgatg cctgacaacc ccatcgaaaa aacccaaaac
    12541 cgctggatag aacagcgcgg cggcaatcct tttattgaaa tcaccgtccc cgaagccggc
    12601 atcaaactca tccaggccgt cggccgcctc atccgcaccg aacaagacta cggccgcgta
    12661 accatcctcg acaaccgcat caaaacacag cggtacggcc aacaattatt ggccggcctg
    12721 ccgccgttta aaaggatagg gtaaacatac cgcccttccc gcaacagaaa ggaaaaattc
    12781 atgaacttta cccgactgct caaccaagtc ttaagcacgg ttcaaaaaaa aggcaacaca
    12841 ttctccgaca gcccgctcaa ttcattcggc ggaggcgcgc tggttgccgg tatcgcctcc
    12901 atgctgctga acggtaaaaa ccgcaaaacc attaccaaaa tcggttcgac cgccgctttg
    12961 ggctacctcg cctaccgggg ctatcagatg tggcagcaaa acaaagggcg ggcaactgta
    13021 acacaaagcg atttccaacc tgccggagaa actgaagaaa catacagccg caccgtattg
    13081 cgcaccatga tagccgccgc cgcttcagac ggcatgatag acgaagccaa acgccggact
    13141 atcgaacagg aaagcggcac agaccccgaa actgccgcat ggctcgccgc cgaataccgc
    13201 ctgcccgcaa gcatcgaaga catcgccgcc gccgtcggca acgatgaggc gttggcggcg
    13261 gaggcctatc tggcggcaag gttggtctgt gccgatttgt cgcggaaaga aaccgtcttc
    13321 ctcgcccgcc tgtcgcaggc tttgaaactg gatgacaatc tggtggagag tttggaaagg
    13381 caattggggt tttgatgaca cagccgggga gggaaaatca tcaaaaaacg gttattccga
    13441 ccccggcctc aaaacggcaa aatatagtgg attaacaaaa accagtacgg cgttgcctcg
    13501 ccttagctca aagagaacga ttctctaagg tgctgaagca ccaagtgaat cggttccgta
    13561 ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataaaa
    13621 gccgccgccc cgaagggcaa acagcggcat aggctgccaa aggcaatatc cgacaagaag
    13681 gcaggctact gcaatgcgtt gccgtgtgaa tgcttgagca agctttcatg tttgtgaaat
    13741 tcccgcccca tataataaga accgaaatag gtcaggtggt cttggtcgcc gtaaagatag
    13801 cggccgtata tttcgaccgt gtttttgggc aggtattttt gtgcgtccac ccaatgcaca
    13861 ttgggaatat ctttaatcaa atcaaagacc gcctgattgc tcttgccgat gtcgcccata
    13921 gcctgaatgg ggcggagata ttggtttgcg gcaaatcttt tcaatttttc ctccctcagg
    13981 ggcgaacggc tgattgatgt gttgtttgca aaaacataga cgggtttgac ggcggctatc
    14041 cttttgacgg tttccctgaa tcgggctggg aacccgggta ttaggaagga ttgcgcttca
    14101 aatctcggca cgggctggcc gcccatcctc aaatcataga attgggcaat gaaaacggct
    14161 tcggcttttt caacttcatc ccggtatttt cgacataacg ggttgtctgc cagcttctca
    14221 tctacccaaa ccaaacactc cgaatcgagg gacaggattt tggctttcca cccttcccgg
    14281 ctgccgacat aatccagaaa accccgcagg tgtccggcgt gcgagtcgcc gagggtcagg
    14341 acggtttccg gaaaatgatt ttctgcagca aggggcgcgc cgggcaacgg gcggaggtgt
    14401 tcctgtttca atatccccct tgcgtacagg ttgtaaccga caagtatcag ggacggggcg
    14461 agatagaggc agaaaaatgc ctttttgaag gtcatcttcc gttttctaag cggctgttca
    14521 atcaaataat aactcaacag ggaaaatccg gccgtcaacg cggcaaccgc cgatacggca
    14581 ggcagtccga gctgtttgtc gcctgtaatg taatgggcga aggcaataaa aatccaatgg
    14641 tacaggtata gggaataaga gattttgccg acaaatacga tggggcttgc cgacaggatg
    14701 cgggtcggaa gtgtcccgta ttgcatactc cggataagca gtgccgtcag caggcaggga
    14761 aggagcaggg tcattcccgg gataaacgga ttgtgtttgt caatcacgaa caggcaggca
    14821 agcaatgcgc cgaagcagag tgatgaaagc aactgccgtt ttccatttgc tgtttgccgt
    14881 ctgccgtttt gcgtttgccc gtaaaccgcc agcagcgaac ctgccaacag ctcgggaaac
    14941 ctcagtgtcg aaaggtaata agtattgggt tggttgagaa tatcggtata aaacccgctt
    15001 ggcaaaaacg atgtggcagt caaaatcaga aataggatga tgctgatgtt acgcagcacc
    15061 cgtagcgatt ttgttttttt gcagcaaaat atcagcaaaa gaggatacag gaggtaatac
    15121 tgttcctcta ctgccaaaga ccagatatgc agtacggggt tctcgtcggc actcaaatcg
    15181 aaatacccct gctgaaaccc cagataaata ttggacaaga aaaccgcaga aagctccacg
    15241 gttttccgca tttggttgaa atcttcgtaa aggaagattt gagaggcaat caccgaagcc
    15301 agcgacacgg ccgcaataaa agcaggataa atccgcttaa tcctgcgggt ataaaaatcc
    15361 cggaaagaaa aagaaccgtt ctgtatttca gcaagaatga tgccggtaat gaggaatcct
    15421 gagatgacaa agaaaatgtc cacccccagg aatcctccgg gcagccagcg gtcattcagg
    15481 tggaaaatca tgacggatag cacggcgacg gcccgcaatc cgtcaatttc aggcctgtat
    15541 cggacagctt gcataaatat cgcccccgta tgtcccgtta tcttaaaaat gccgtctgaa
    15601 cgcgcgttca gacggcatcg gtttcagtaa acgctcaaat ccgtttcgcc aacgcttcag
    15661 ccttgcccac atacagcgcg ggggtcagct caagcaattt ggctttggct tcggcgggga
    15721 tttccagcaa tccgataaag cctttcagca cttcgggcgt gatgccgcct ttgccgcgcg
    15781 tcaggtcttt cagtttttcg taaggattgg cgacaccgta acggcgcatt acggtttgaa
    15841 tcggctcggc gagcagctcc caagtggcat ccaaatcggc ggcaagcgcg gcggggttgg
    15901 gttcgagctt gttcagaccg cgcaggtggg cggcgaaacc caatacggca tagcccacgc
    15961 ctacgcccat attgcgtaag acggtgctgt cggtcaggtc gcgctgccag cgggaaatcg
    16021 gcagtttttc ggacaaaaag cccaatacgg cgtttgccat accgaggttg ccctcggagt
    16081 tttcaaagtc gatggggttg actttgtgcg gcatggtgga agaaccgact tcgcctgctt
    16141 tgactttttg tttgaagtaa cccaatgaaa tataacccca aacgtcgcgg ttaaagtcga
    16201 tgagaatcgt gttgatgcgg ctgagggttt ggaagaattc cgccatatag tcgtgcggtt
    16261 cgatttggat ggtgtagggg ttgaaggtca gaccgaggct gatttcgacg aagttgcggc
    16321 agtgggtttc ccaatctaca tcaggatagg cgaccatatg ggcgttgtag ttgccgaccg
    16381 cgccgttgat tttgccgagg aactcttgcg cttgcaggtt tttaaactgg cgttgcaggc
    16441 ggtacacgac attggcggtt tctttgccca aagtggtcgg cgtggcgggc tggccgtggg
    16501 tgcggctcat catcgggacg gcggcaaggt cgtgcgccat agcggtcagt ttttcgatga
    16561 tttcggccag cttcggcagc aaaacagcct cacgcgcttc ttgcagcatt aaagcgtggg
    16621 acaggttgtt gatgtcttcg ctggtgcagg cgaagtggac gaactcactc acggcggcga
    16681 cttccggcac ttcggcaaaa cgttttttca gccaatattc aatggctttg acatcatgat
    16741 tggtcgtggc ttcgatggct ttgacagcgg ctgcgtcttc caatgaaaag ttttcaatca
    16801 ccgtgtcgat ttcggcaagc gtttcggcac tgaagggcgg cacttcgaca atcttcggct
    16861 cggcggccag ggctttgagc cagtttaatt cgactttgac gcgcgccttc atcaggccgt
    16921 actctgaaaa aatcgggcgc aatgcttcaa cggattgggc ataacggccg tctaaagggg
    16981 aaagcgaggc gatggggttg atcatttcgg catcctgttc gggaaagaca tcaaaaattg
    17041 ttaaattgtt tgcaattata catcactctc aggacgctat gccgtctgaa gcccgtgttc
    17101 cgacagatac gcaacgattt cgtcttcatc gtccgatatg gcaaacaaag agaccgcccc
    17161 ttcggaaatc agcccgcgcg ccaaaagctg cgcgtttatc cactccgcca agcccgacca
    17221 aaacgccttt ccgaccaaaa caatcggacg cggcggcact ttgcccgtct gcaccaaggt
    17281 caggatttca aacaattcgt ccagcgtccc gaagccgccc ggcatcacga catatgcttg
    17341 ggaatagcgg aaaaacaccg ccttgcgttc ggcaaaacgg gaaaaccgca aggcgatgtc
    17401 ctgatacgga ttcggtttct gctcgtgcgg caaaacgatg ttcagcccca ccgaaaccga
    17461 cttccctgca aacgcgccct tgtttgccgc ctccataatc cccggcccgc cgcccgaaat
    17521 gacggcaatg cccgaatccg acagccgccg cgccagacgg caggcgaacg cataatccgc
    17581 atgattctgc ggcgtgcgcg cgctgccgaa gatactgact gccggaaaca cgcccgccaa
    17641 tgcttcgtct gcctgcctgc gttcggcatc ataacgtgcc tgctccggca cacggtttgt
    17701 attctccatt ccatcctccg ttcaaaaaca gcgattgtac accgtcaaaa a
//
LOCUS       DAWFIE010000039        17169 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWFIE010000039 DAWFIE010000000
VERSION     DAWFIE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17169
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.039"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            597..1472
                     /gene="prpB"
                     /locus_tag="WGQ71_001206"
     CDS             597..1472
                     /gene="prpB"
                     /locus_tag="WGQ71_001206"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ0707218.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADVVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            1558..2712
                     /gene="prpC"
                     /locus_tag="WGQ71_001207"
     CDS             1558..2712
                     /gene="prpC"
                     /locus_tag="WGQ71_001207"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ0707219.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKAKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPEREGHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     EEETIGGHFLHLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            3038..3982
                     /locus_tag="WGQ71_001208"
     CDS             3038..3982
                     /locus_tag="WGQ71_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HEZ0707220.1"
                     /translation="MYHKKFDKEILLKIKELYRYDNWHAVLAILYDYLIIIFSIFLSE
                     QSYWFLPLTILLIGSRQRALATILHEASHSALTKNKKFGQILGTYFSGYLIFQSWNSY
                     CVSHVKNHHPKLGTDSDPDYKYYKDSGIFQTYSRIEFFWRFCVSQILCLKLFRNFKYL
                     IKNRLFSDITRTELFKLLLSQLILFVIGTYFVGYKFYVIYWLLPYLTAFQMITWFIEL
                     SEHYPMIQTAKNNLEASRNRFSHAIEHFFTGVHGENYHLIHHLFPAIPFWNLEKAHKI
                     LMKDFDYQEVNKDFGGIFFSKNNNAPMWKVLWSKYEAA"
     gene            3969..4880
                     /locus_tag="WGQ71_001209"
     CDS             3969..4880
                     /locus_tag="WGQ71_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0707221.1"
                     /translation="MKLRDLLMGIFLAVSAALLNATIGIFSKILMEQGLSVQHIAFLK
                     TLTGFVFISILLCRTGFTRQIADISRKKEAILPLLLKVAICAFFGIYTLFFFETTAYQ
                     YGNAANVVVVLMASAAVSALILDSILLDERICISSVVGVGLAVLGIAMISWTGEGSLG
                     LILNAALAGSGYGCFSVLIKKFGLNGGIYLTRILMFFGSIFLFIPSLEGIEDIHWQWS
                     FIPPLLALSLLPTILGFYCTTKALDYLSAAKVQVTELAEPLFAAVLAWLFLNEIPEGR
                     FFVGAILIIAGIVSINGLYRPLLKRIE"
     gene            4971..7577
                     /gene="acnD"
                     /locus_tag="WGQ71_001210"
     CDS             4971..7577
                     /gene="acnD"
                     /locus_tag="WGQ71_001210"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ0707222.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNRQIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRFGLKRKPWVKTSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            8380..9549
                     /gene="prpF"
                     /locus_tag="WGQ71_001211"
     CDS             8380..9549
                     /gene="prpF"
                     /locus_tag="WGQ71_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ0707223.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDSADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTTTKAVMSRSARVMMEGWVRVPEDCF"
     gene            9963..11162
                     /locus_tag="WGQ71_001212"
     CDS             9963..11162
                     /locus_tag="WGQ71_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0707224.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAVGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELVGIL"
     gene            11520..12140
                     /locus_tag="WGQ71_001213"
     CDS             11520..12140
                     /locus_tag="WGQ71_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ0707225.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVIDMTDGTELVRQGCGDTAVFGL"
     gene            complement(12302..12745)
                     /locus_tag="WGQ71_001214"
     CDS             complement(12302..12745)
                     /locus_tag="WGQ71_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ0707226.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAGTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            12919..13287
                     /locus_tag="WGQ71_001215"
     CDS             12919..13287
                     /locus_tag="WGQ71_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0707227.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAVEPFLPA"
     gene            13332..14486
                     /locus_tag="WGQ71_001216"
     CDS             13332..14486
                     /locus_tag="WGQ71_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0707228.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTPDLPGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTVFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(14543..15415)
                     /locus_tag="WGQ71_001217"
     CDS             complement(14543..15415)
                     /locus_tag="WGQ71_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ0707229.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPDILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVEELWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            15500..16312
                     /locus_tag="WGQ71_001218"
     CDS             15500..16312
                     /locus_tag="WGQ71_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ0707230.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGEVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         4103 a         4350 c         4595 g         4121 t
ORIGIN      
        1 cttgtcctga tttaaattca atccactata tatttaaatt taattaaata atataaaaat
       61 caaatagata acaaactaca tcggattgct taaaaataat acattgtttt tatgtataaa
      121 atattttata agttttcagg attttgatta ttgaaaattt ttctcaattt cctgttaatt
      181 ttatcggaaa caaacaagtt aaaattaatt cattttaatc atagtattaa aataaaatat
      241 taaaatcatg taatgaacct ccttaaaaat gtttgacatt ttcagtcttg tgttttagat
      301 tagcgaaaaa taaaactaca caatactaca aaggaacatt actatgaaac caattcagat
      361 gttttcccct tttctgaata atccccttgt tttcttcttg tctgcggttt tgccgcataa
      421 ttccgaacgg tctgctgttt ttctttgatt cgttttaaat atcaataaga taatttttcc
      481 catatatttt taatgattgg attgggatgc ccgacgcgtc ggatggctgt gttttgccgt
      541 ccgaatgtga tggaagcctg tccatactga aaaaaagtct ataaaggaga aatatgatga
      601 gtcaacactc tgccggagca cgtttccgcc aagccgtgaa agaatcgaat ccgcttgccg
      661 tcgccggttg cgtcaatgct tattttgcac gattggccac ccaaagcggt ttcaaagcca
      721 tctatctgtc cggcggcggc gtggcagcct gttcttgcgg tatccctgat ttgggcatta
      781 ccacaatgga agatgtgctg atcgacgcac gacgcattac ggacaacgtg gatacgcctc
      841 tgctggtgga catcgatgtg ggttggggcg gtgcattcaa tattgcccgt accattcgca
      901 actttgaacg cgccggtgtt gcagcggttc acatcgaaga tcaggtagcg caaaaacgct
      961 gcggccaccg tccgaacaaa gccattgtat ctaaagatga aatggtcgac cgtatcaaag
     1021 ctgccgtaga tgcgcgcgtt gatgagaact tcgtgattat ggcgcgtacc gatgcgctgg
     1081 cggtagaagg tttggatgcc gctatcgaac gcgcccaagc ttgtgtcgaa gccggtgcgg
     1141 acatgatttt ccctgaagcc atgaccgatt tgaacatgta ccgccaattt gcagatgtgg
     1201 tgaaagtgcc cgtgttggcg aacattaccg agtttggttc cactccgctt tatacccaaa
     1261 gcgagctggc tgaaaacggc gtgtcgctgg tgctgtatcc gctgtcatcg ttccgtgcag
     1321 caagcaaagc cgctctgaat gtttacgaag cgattatgcg cgatggcact caggcggcgg
     1381 tggtggacag tatgcaaacc cgtgccgagc tgtacgagca tctgaactat catgccttcg
     1441 agcaaaaact ggataaattg tttcaaaaat gatttaccgc tttcagacgg tctttcaaca
     1501 aatccgcatc ggtcgtctga aaacccgaaa cccataaaaa cacaaaggag aaataccatg
     1561 actgaaacta ctcaaacccc gaccttcaaa cctaagaaat ccgttgcgct ttcaggcgtt
     1621 gcggccggta ataccgcttt gtgtaccgtt ggccgtaccg gcaacgattt gagttatcgc
     1681 ggttacgaca tcttggattt ggcacaaaaa tgtgagtttg aagaagttgc ccacctgctg
     1741 attcacggcc atttacccaa caaattcgag ctggccgctt ataaagccaa gctcaaatcc
     1801 atgcgcggcc tgcctatccg tgtgattaaa gttttggaaa gcctgcctgc acatacccat
     1861 ccgatggacg tgatgcgtac cggcgtatcc atgctgggct gtgttcatcc tgaacgtgaa
     1921 ggccatccgg aaagcgaagc gcgcgacatt gccgacaaac tgatcgccag cctcggcagt
     1981 atcctcttgt actggtatca atattcgcac aacggcaaac gcattgaagt tgaaagcgaa
     2041 gaagagacca tcggcggtca tttcctgcac ctgttgcacg gcaaacgccc aagcgaatca
     2101 cacatcaaag ccatgcacgt ttcactgatt ctgtatgccg aacacgagtt caacgcttct
     2161 acctttaccg cccgcgtgat cgccggtaca ggctctgata tgtactccag cattaccgga
     2221 gcaatcggcg cgttgaaagg tccgaaacac ggcggcgcga acgaagtggc ttacgatatt
     2281 caaaaacgct accgcaatgc cgacgaagct gaagccgaca tccgcgaacg catcggccgc
     2341 aaagaaatcg tgatcggttt cggtcatccg gtgtacacca tttccgaccc tcgcaacgtt
     2401 gtcattaaag aagtggcacg cggtttgagc aaagaaaccg gcgatatgcg cctctttgac
     2461 attgccgaac gtttggaaag cgtgatgtgg gaagagaaaa aaatgttccc gaatctggac
     2521 tggttctctg ccgtttccta ccaaaaattg ggcgtaccga ccgctatgtt cacaccgctg
     2581 ttcgtaattt cccgtacaac cggttggagc gcacacgttc ttgagcaacg caaagacggc
     2641 aaaatcatcc gtccgagcgc aaactacacg ggccctgaag atttggcgtt tgtggagatt
     2701 gaagaacgct gagacggaac acaaccgtag gagaccacca atcggatttc agacgacgtg
     2761 tagcgcgggc tttgcctgcg gaaaactgaa acccgcctac ggttccgctt ttcagacggc
     2821 ctgttgaaag atgcgagaga gaaggttgtc tgaaaaacca gccgcaacgt ctgccgtcat
     2881 tcccaccccc gcctaagcga ggacaggtcg cggcgggaat ggcggaggca gcgcggcaac
     2941 ttttttcacg gttctgccaa aatatgctat gaaagaatgt tcgtcagcat aatggagatg
     3001 aagccgatat tataactaat acggagaatt gtgggatatg taccataaga aatttgataa
     3061 agaaattctt ttaaaaatta aagaattata tagatacgat aattggcatg ctgtacttgc
     3121 tatattatat gattatttaa taataatttt ttctatattt ttatctgagc agtcatattg
     3181 gtttctccct ttaacgattt tattaatagg aagcaggcaa agggcgttgg caacaatttt
     3241 acatgaagcc tctcattctg ccttaactaa aaataagaag tttggacaaa ttttaggtac
     3301 atatttttct ggatatttaa tttttcaaag ttggaatagt tattgtgttt ctcatgtgaa
     3361 gaatcatcat ccaaagttag ggactgattc ggatcctgat tataagtatt ataaagactc
     3421 aggtattttt cagacatatt caagaattga atttttctgg agattttgtg tctctcaaat
     3481 tttatgtttg aaattattta ggaattttaa atatttaatt aaaaatagat tattttcgga
     3541 tattacaaga acagagttgt ttaagctact tctttctcaa ttaattctat ttgttatcgg
     3601 tacatatttt gtagggtata aattttatgt aatttattgg ttattaccat atttaactgc
     3661 attccagatg ataacttggt ttattgagtt gtccgagcat tatccaatga tacaaactgc
     3721 caaaaataat ttagaagcgt caagaaatag gtttagtcat gcaatagagc attttttcac
     3781 gggggtacat ggagaaaatt atcatctaat acatcattta tttccagcca ttccattttg
     3841 gaatttggaa aaagcacata agatattaat gaaagatttt gattatcaag aggtaaataa
     3901 agattttgga gggattttct tttcgaaaaa caataatgca cctatgtgga aagtattatg
     3961 gagtaaatat gaagctgcgt gatttactga tgggaatatt cttggcagtt tctgcggccc
     4021 ttctgaatgc aaccatcggc atattcagca agatattgat ggagcagggc ttgtctgttc
     4081 agcatattgc atttttgaaa actttgacag gtttcgtgtt tatcagcatt ttgctttgcc
     4141 gtaccggttt taccagacag attgcggata tttcaagaaa gaaagaggca attttgccgt
     4201 tgctgttaaa agtagcaatt tgtgcttttt tcggaattta tacgttgttt ttctttgaaa
     4261 ccacagctta tcaatatggc aatgctgcga atgtagtagt tgtattaatg gcatcggctg
     4321 ccgtatctgc cttgatattg gacagcatac tgttagatga acgtatttgc atttcttcag
     4381 tcgtcggtgt gggtttggca gtattgggga tcgcaatgat ttcttggact ggagaaggaa
     4441 gtttagggtt gattctgaat gccgcactgg cgggctcggg ctacggttgt ttttccgttt
     4501 tgattaagaa attcggccta aacggcggta tttatttgac acggatattg atgttttttg
     4561 gaagtatttt tttgtttatc ccttcattgg aaggtattga ggatatacat tggcaatggt
     4621 cttttattcc gccactcttg gcattgtctt tattgccgac gattttagga ttttattgta
     4681 caactaaagc attggattat ttgagtgctg cgaaggtaca ggtaactgaa ttggccgagc
     4741 cattgtttgc tgccgtactg gcttggttgt ttttgaatga aataccggaa ggacgcttct
     4801 ttgtcggcgc cattctgatt attgccggta ttgtgtctat caatgggctg tatcgaccat
     4861 tgttgaagcg aattgaataa tttttatttc ccaaaatttc agacgacctc ttcaacaaac
     4921 gtcgtctgaa accccaaaac caaaaaaata atagatttag gagaaccgac atggctgcca
     4981 accaacgtta ccgcaaaccg ctgcccggta cggatttgga atactacgac gcgcgtgcgg
     5041 cgtgtgagga catcaagccc ggctcttacg acaagctgcc ttacacgagc cgcattttgg
     5101 cggagaattt ggtcaaccgc gcggacaaag tcgatttgcc gacgctgcaa agctggctgg
     5161 gtcagctgat tgagggaaaa caggaaatcg actttccttg gtatccggcg cgggtggtgt
     5221 gccacgatat tctggggcag accgcgttgg tggatttggc aggtctgcgc gatgcgattg
     5281 ccgaaaaagg cggcgatcct gccaaagtga atccggtggt gcaaacccag ctcatcgtcg
     5341 accactcttt ggccgttgaa tgcggcggct acgaccccga tgccttccgc aaaaaccgcc
     5401 aaatcgaaga cagacgtaac gaagaccgtt tccacttcat caactggacg aaaaccgcct
     5461 ttgaaaatgt ggacgtgatt ccggcgggca acggcatcat gcaccaaatc aatctggaaa
     5521 aaatgtcgcc cgtcgtccaa gtcaaaaacg gcgtggcgtt ccccgatacc tgcgtcggca
     5581 cggattcgca cacgccgcac gtcgatgcgc tgggcgtgat ttccgtgggc gtgggcggat
     5641 tggaagcgga aaccgtaatg ctgggacgcg cgtccatgat gcgcctgccc gatattgtcg
     5701 gcgttgagct gaacggcaaa cggcaggcgg gcattacggc gacggatatt gtgttggcac
     5761 tgaccgagtt tctgcgcaaa gaacgcgtgg tcggggcgtt tgtcgaattc ttcggcgagg
     5821 gcgcgagaag cctgtctatc ggcgaccgcg cgaccatttc caacatgacg ccggagttcg
     5881 gcgcgactgc cgcgatgttc gctattgatg agcaaaccat tgattatttg aaactgaccg
     5941 gacgcgacga cgcgcaggtg aaattggtgg aaacctacgc caaaaccgca ggcttgtggg
     6001 cagatgcctt gaaaaccgcc gtttatcctc gcgttttgaa atttgatttg agcagcgtaa
     6061 cgcgcaatat ggcaggtcca agtaacccgc atgcccgttt tgcgaccgcc gatttggcgg
     6121 cgaaagggct ggcgaagcct tacgaagagc cttcagacgg ccaaatgccc gacggcgcgg
     6181 tcatcatcgc cgcgattacc agttgcacca acacttccaa cccgcgcaac gtcgttgccg
     6241 ccgcgctctt ggcgcgcaac gccaaccgct tcgggctgaa acgcaaaccg tgggtcaaaa
     6301 cctcgtttgc ccccggttcg aaagtggcgg aaatttattt gaaagaagca ggcctgctgc
     6361 ccgaaatgga aaaactcggc ttcggtatcg tcgccttcgc ctgcaccacc tgcaacggca
     6421 tgagtggcgc gctggatccg aaaatccaga aagaaatcat cgaccgcgat ttgtacgcca
     6481 ccgccgtatt atcaggcaac cgcaacttcg acggccgtgt ccatccgtat gcgaaacagg
     6541 ctttcctcgc ttcgcctccg ttggtcgttg cctacgcgct ggcaggcagt atccgtttcg
     6601 atattgaaaa cgacgtactc ggcgttgcag acggcaagga aatccgcctg aaagacattt
     6661 ggcctgccga tgaagaaatc gatgccgtcg ttgccgaata tgtgaaaccg cagcagttcc
     6721 gcgatgtgta tgtaccgatg ttcgacaccg gcacagcgca aaaagcaccc agtccgctgt
     6781 acgattggcg tccgatgtcc acctacatcc gccgtccgcc ttactgggaa ggcgcgctgg
     6841 caggggaacg cacattaaga ggtatgcgtc cgctggcgat tttgcccgac aacatcacca
     6901 ccgaccacct ctcgccgtcc aatgcgattt tggccgtcag tgccgcaggc gagtatttgg
     6961 cgaaaatggg tttgcctgaa gaagacttca actcttacgc aacccaccgt ggcgaccact
     7021 tgaccgccca acgcgctacc ttcgccaatc cgaaactgtt taacgaaatg gtgaaaaacg
     7081 aagacggcag cgtgcgccaa ggctcgttcg cccgcgtcga acccgaaggc gaaaccatgc
     7141 gcatgtggga agccatcgaa acctatatga accgcaaaca gccgctcatc atcattgccg
     7201 gtgcggacta tggtcaaggc tcaagccgcg actgggctgc aaaaggcgta cgcctcgccg
     7261 gcgtagaagc gattgttgcc gaaggcttcg agcgtatcca ccgcaccaac cttatcggca
     7321 tgggcgtgtt gccgctgcag ttcaaacccg acaccaaccg ccataccctg caactggacg
     7381 gtacggaaac ctacgacgtg gtcggcgaac gcacaccgcg ctgcgacctg accctcgtga
     7441 ttcaccgtaa aaacggcgaa accgttgaag ttcccgttac ctgccgcctc gatactgcag
     7501 aagaagtatt ggtatatgaa gccggcggcg tgttgcaacg gtttgcacag gattttttgg
     7561 aagggaacgc ggcttagagg tcgtctgaaa agcaagacgt agcgtgggtc gggttcaaca
     7621 ttttgctcat tcacgtaatt ctcgatatgg caggcatcta ctgtaaatcg tcattcccgc
     7681 gcaggcggga atccagaaag tggaattgag gaaaccttat ttatccgatg agtttctgtg
     7741 cggacaaatc tggattcccg cctgcgcggg aatgacgggg tttaataatc tgccgtatca
     7801 caacacagta gccgtagatt gtggcgaacc ccgacagttt gcggaatcaa acggctttgt
     7861 cggagtggca gcctaatgta cttctggaaa gtgggtgtag cgtgggcttt gcccgcgaaa
     7921 taaaggctga attgacatgg tatagaggat taacaaaaat cgggacaagg cggcgaagcc
     7981 gcagacagta cagatagtac ggaaccgatt cacttggtgc ttcagcacct tagagaatcg
     8041 ttctctttga gctaaggcga gccaacgctg tactggtttt tgttaatcca ctataaattt
     8101 aatccactat actgtaaatc gtcattcccg cgcaggcggg aatccagaaa gtggaattga
     8161 ggaaaccttt ttatccgatg agtttctgtg cggataaatc tggattcccg cctgcgcggg
     8221 aatgacgggg tttaataatc tgccgtatca caacacagta gccgtagatt ggggcgaacc
     8281 ccgacagttt gcggaatcaa acggctttgg tcggagtggc agcctaatcc actataaaaa
     8341 tcgtgggcag agcccacgct acataaggag aatctagaaa tgccgcaaat taaaattccc
     8401 gccgtttact accgtggcgg tacatcaaaa ggcgtgtttt tcaaacgttc cgacctgccc
     8461 gaggcggcgc gggaagcggg aagcgcacgc gacaaaatcc tcttgcgcgt actcggcagc
     8521 ccggatccct acggcaagca gatagacggt ttgggcaacg ccagttcgtc caccagcaaa
     8581 gccgtgattt tggacaagtc cgaacgcgcc gatcacgatg tcgattacct tttcgggcaa
     8641 gtttccatcg acaaaccttt tgtcgattgg agcggcaact gcggcaacct caccgccgcc
     8701 gtgggcgcat tctccatcga acagggcttg gtcgataaag gcaagattcc ttcagacggc
     8761 atctgcacgg tcaaaatctg gcagaaaaac atcggcaaaa ccattattgc ccatgtaccg
     8821 atgcaaaacg gcgcagtttt ggaaacaggc gattttgagc tcgacggcgt aacgttcccg
     8881 gcagccgaag tacaaatcga atttcttgat tcagccgacg gcgaaggcag tatgttccca
     8941 accggcaatt tggtcgatga aattgatgtg ccgaatatag gccgtttgaa agccacgctc
     9001 atcaacgcgg gcattccgac cgttttcctg aatgccgccg acttgggcta cacgggcaaa
     9061 gagttgcaag acgacatcaa caacgatgcc gcggctttgg aaaaattcga gaaaatccgc
     9121 gcttacggtg cgctgaaaat gggtctgatc agcgacgtat ccgaagctgc cgcccgcgcg
     9181 cacacgccga aagtcgcctt cgtcgcgccc gccgccgatt acaccgcctc cagtggcaaa
     9241 accgtgaacg ccgccgacat cgatttgctg gtacgcgccc tgagcatggg caaactgcac
     9301 cacgcgatga tgggtaccgc ctctgttgcc attgcgaccg ccgccgccgt gcccggtacg
     9361 ctggtcaacc ttgccgcagg cggcggaacg cgtaaagaag tgcgcttcgg gcatccttct
     9421 ggcacattgc gtgtcggtgc agccgccgaa tgtcaggacg gacaatggac gaccaccaaa
     9481 gcggttatga gccgcagcgc acgcgtgatg atggaaggtt gggtcagggt gcctgaggat
     9541 tgtttttaaa tcgacgtagc gtgggtttgc tcgcgagcca taaaaaggtc gtctgaaaaa
     9601 caagtaaaca tcaaatcact gaccattcct ttcccttgcc ctgtggcgga aggcggcaaa
     9661 tcacaaggaa gaacacggaa accccgataa aagacagctt cccgtattac cgtcattccc
     9721 gcgcaggcgg gaatccagac ctgtcaatat ggaagattgg caggggaaaa taggtttcgt
     9781 gagttctaca ttctggattc ccgccacagc ctgtccttgc gtaggcgggg acggaataac
     9841 gataggaaat gcggcatatg ctttgcccaa agaggccgtc tgacacacac tccgcccaac
     9901 gcccatcctt tttcagacga ccccacacca aaaaacaacc acaaactaca aggagaaaca
     9961 tcatgtccga ccaactcatt cttgttctga actgcggcag ttcatcgctc aaaggtgccg
    10021 ttatcgaccg caaaagcggc agcgtcgtcc taagctgcct cggcgaacgc ctgaccacgc
    10081 ccgaagccgt cattaccttc aacaaagacg gcaacaaacg ccaagttccc ctgagcggcc
    10141 gcaactgcca tgccggcgcg gtgggtatgc tgttgaacga actggaaaaa cacggactgc
    10201 acgaccgcat caaagccgtc ggccaccgca tcgcccacgg cggcgaaaaa tacagcgagt
    10261 ctgttttgat cgaccaggcc gtaatggacg aactcaatgc ctgcattccg cttgcgccgc
    10321 tgcacaaccc cgccaacatc agcggcatcc ttgccgcaca ggaacatttc cccggcctgc
    10381 ccaatgtcgg cgtgatggat acttcgttcc accaaaccat gccggagcgt gcctacactt
    10441 atgccgtgcc gcgcgagttg cgtaaaaaat acgctttccg ccgctacggt ttccacggca
    10501 ccagtatgcg ttacgttgcc cctgaagccg cacgcatctt gggcaaacct ctggaagaca
    10561 tccgcatgat tattgcccac ttaggcaacg gcgcatccat taccgccatc aaaaacggca
    10621 aatccgtcga taccagtatg ggtttcacgc cgatcgaagg tttggtaatg ggtacacgtt
    10681 gcggcgacat cgatccgggc gtatacagct atctgacttc ccacgccggg atggatgttg
    10741 cccaagtgga tgaaatgctg aacaaaaaat caggtttgct cggtatttcc gaactttcca
    10801 acgactgccg caccctcgaa atcgccgccg acgaaggcca cgaaggcgcg cgcctcgccc
    10861 tcgaagtcat gacctaccgc ctcgccaaat acatcgcttc gatggctgtg gcctgcggcg
    10921 gcgttgacgc actcgtgttc accggcggta tcggcgaaaa ctcgcgtaat atccgtgcca
    10981 aaaccgtttc ctatcttgat ttcttgggtc tgcacatcga caccaaagcc aatatggaaa
    11041 aacgctacgg caattcgggc attatcagcc cgaccgattc ttctccggct gttttggttg
    11101 tcccgaccaa tgaagaactg atgattgcct gcgacactgc cgaacttgtc ggcatcttgt
    11161 agccaaaaaa gggacgagtc cgcacaatgc cgtctgaaac cccaaacgcc cgattaggct
    11221 gatgaggatt ttagacggca ttgttcattt ttttgttatc ttgcattttt gtgcggacgg
    11281 tggaatttca tcctgtaaac ataaatattt gtcggaaaac agaaaccctc cgccgccatt
    11341 tctacgaaag caggaaacca gcaacgcaaa gcgacaggga tttgttggaa atgaccgaaa
    11401 ccgaacgaac cggattcccg cctgcgcggg aatgacggga ttttctgttt ttgtggaaat
    11461 gacgggattt tgaatttcgg gcgtacaata cggaaaacat gacgataagg aaaaaaacta
    11521 tggcacagtt tttcgctatt catcccgaca acccccaaga acgcctcatc aagcaggcgg
    11581 tcgaaatcgt caataaaggc ggcgtggtcg tttatccgac cgattcctgt tatgccttgg
    11641 gttgcaaact cggcgataag gcggcgatgg aacgcatact ctccatccga aaaatcgatt
    11701 tgaaacacca cctgaccctg atgtgcgccg atttgagcga gttgggcaca tacgccaaaa
    11761 ttgacaacgt acagtttcgt cagcttaaag ccgccacacc cgggccttat acttttattt
    11821 tacaggcgac gaaggatgtg ccggcgcgca cgctgcaccc gaaacgcaaa accatcgggc
    11881 tgcgtattcc cgataatgcc attgcacaag ccctgctggg ggaattgggc gagccgcttt
    11941 taagctgcac cctgatgctg cccgaagacg gcgaaccatt gaccgatcct tatgaaatcc
    12001 gcgagcgttt ggaacacgcc gtcgatttgg tgattgacgg cggctggtgc ggaaccgagc
    12061 cgaccaccgt catcgacatg accgacggta cggaattggt gcgccaaggt tgcggcgata
    12121 cggcggtgtt cggtttgtag ggaaaccgat gccgtctgaa gcatcggctg ttcagacggc
    12181 attgcgcgcc ttgccggcgg cagtccgaaa tgccggcgcg tatcgcgctc ggtcggaata
    12241 tccgtttgaa acggcaaaca aaatgccgtc cgaacgcggg ttcggacggc attttgatgc
    12301 attactgcac cgcaatcgga attctcggtt cgtagagcag gtcgtaggtc ggcttgttga
    12361 gcaggtcttg gagcgtgaaa ccgtccaaat acgtgaaaaa cgacttcatc gcgccgccga
    12421 gtatgcctgt cagacggcag gacggtgtga tcaggcattc gttgttctcg cccatgcact
    12481 cgaccagctg catcggttcg aggtggcgga cgaccgagcc gatgttgatg cggtcgggcg
    12541 gtgcggcaag ccgcagaccg ccgccttttc cgcgcacgct gtggaggaag ccgcctttga
    12601 ccagcgcggt aacgactttc atcagatggc ttttggaaat gccgtaagtt ccggcgatgg
    12661 tactgatgtt gaccagcgca tcgtcgttga tggcagtgta gataaggacg cgcagcccgt
    12721 agtccgtatg ttgtgtcaaa tacatgattt tctcggtatg gatcgttatt cttatcggta
    12781 cggtttaagg ttcacggaca ataccttaat ggttgaaacc ctgtccgtcg gggcggtaga
    12841 atgcagcctg tctgcggcgg tatgccgtct gaaacatccg cgctaccgtt tgataatttg
    12901 ttattgtaac tcaaaatcat gaaaccgttg aaacgacatc ccgcccttat cgggctttcg
    12961 cgtgaccacc accattcgct ttccctgtgc gtgcgtctgt tgcggacgcc ggaagaacgg
    13021 catcgggacg aactcgaacc gcatttttcc gaattggaaa cccattttcg cgaagaagaa
    13081 gccaagtttg ccccaatttg gcagaatgtc gcccccgaat tgaaacaacg tttcgagaaa
    13141 gaccacgccc gactgcggca gatgatggca agccccgaat acggtaacgc ggcgtggaat
    13201 accgcttttg ccacaaccct gcgcgatcac gcgcgctttg aagaacgcga gctgtttccc
    13261 gccgttgaac cgtttttgcc ggcatgattc cgttttgcgg caaatatatt aatgataaac
    13321 aaggaacaca catgaaattt accaagcacc ccgtctgggc aatggcgttc cgcccgtttt
    13381 attcactggc ggctctgtac ggcgcattgt ccgtattgct gtggggcttc ggctacacgg
    13441 gcacgccgga tctgcccggt ttttactggc acgcgcatga gatgatttgg ggttatgccg
    13501 gcctcgtcgt catcgccttc ctgctgaccg ccgtcgccac ttggacgggg cagccgccca
    13561 cgcggggcgg cgttctggtc ggcttgactg tcttttggct ggctgcgcgg attgccgcct
    13621 ttatcccggg ttggggtgcg tcggcaagcg gcatactcgg tacgctgttt ttctggtatg
    13681 gcgcggtgtg catggctttg cccgttatcc gttcacagaa tcaacgcaac tatgttgccg
    13741 tgttcgcgct gttcgtcttg ggcggcacgc atgcggcgtt ccacgtccag ttgcacaacg
    13801 gcaacctagg cggactcttg agcggattac agtcgggctt ggtgatggtg tcgggtttta
    13861 tcggtctgat tggtacgcgg attatttcgt tttttacgtc caaacggttg aatgtgccgc
    13921 agattcccag tccgaaatgg gtagcgcagg cttcgctgtg gctgcccatg ctgaccgcca
    13981 tgctgatggc gcacggcgtg atgccttggc tgtcggcggc tttcgcgttt gcggcaggtg
    14041 tgatttttac cgtgcaggtg taccgctggt ggtataaacc cgtgttgaaa gagccgatgc
    14101 tgtggattct gtttgccggc tatctgttta ccggattggg gctgattgcg gtcggcgcgt
    14161 cttatttcaa acccgctttc ctcaatctgg gtgtgcatct gatcggggtc ggcggtatcg
    14221 gcgtgctgac tttgggcatg atggcgcgta ccgcgctcgg tcatacgggc aatccgattt
    14281 atccgccgcc caaagccgtt cccgttgcgt tttggctgat gatggcggca accgccgtcc
    14341 gtatggttgc cgtattttct tccggcactg cctacacgca cagcatacgc acctcttcgg
    14401 ttttgtttgc acttgcgctt ttggtgtatg cgtggaagta tattccttgg ctgattcgtc
    14461 cgcgttcgga cggcaggccc ggttgagaca aaccgccgca gatttcgggt ctgggcggtt
    14521 tgcttttcag acggcagggc ggtcagttgc cgtccagcca gcggtcgcgt gtggttttgg
    14581 cttcttcaaa atagcggtac agggcttcgc ggtcgtcggt ggtcaggatg tttgccaaaa
    14641 cgtccaactg tttgcccaag ccttgaacca gttgcagcag gctgtctttg ttggcaaggc
    14701 agatgtccgc ccacacggcg ggatgaccgg aggcgatgcg ggtgaagtcc cgaaagcccg
    14761 tggcggcgaa tttcagatat tcctgtccgt cggggtggtc gagaatctgg tggacatagg
    14821 cgaaggcggt caggtggggc atatgggaga cggcagcgaa aaccgcgtcg tggcgttgcg
    14881 cgtccatcgt ataaatttcc gcaccgaccg cgtgccacag ttcttctacc aaggcaatgc
    14941 cgtctgaatg ttcgccgccg tgtggcgtga tgatgagttt tctgtggcgg aacagcccga
    15001 actgcgcggc ttgcgcaccg cttctgtccg aaccggcaat tgggtgggcg gcgatgcagt
    15061 ggtgcagacg gtcgggcaga cagcggcgga aggcttcgat gaccgaagat ttggtgctgc
    15121 cgacatcgga aatccaagtg tgttccggca aaacggggcg cagcgcggtc aaaatgtcgg
    15181 gaacggtggc gacgggcgtg gcaatcagta ccaagtccgc accgccgatg ctgtccgcgt
    15241 cgatggcaac ggacgcacgg tcaatcacgc cgcgttccaa tgcacgttcg aggttgtcgc
    15301 ggtcggtgtc gataccggta acggtgcgga cgagtccctg ccttttgagg tcgagaacga
    15361 acgaaccgcc gatcagccct acaccgatga gggcaatatg gttcaaaatg ggcatttgtg
    15421 taaacggttt tcgcaaagta ccgtcatggt agcctatcgg cggaatatgc cgcaaggtcg
    15481 gcaggaaaaa ggagaagaaa tggacaaaat cagagttgcc gccgtgcaga tggtgtcggg
    15541 cgtgtcgccg gaaaccaacg tcgccgccat gaaacgcctg gtcgcacggg cggcggagca
    15601 gggtgcggat tgggtgctgc tgcccgaata ttgggtgctg atgggcgcaa acgataccga
    15661 caaactcgcg cttgccgagc ctttgggcgg cggacgcttt cagacggcat tgagcgaaac
    15721 ggcgaaagaa tgcggcgtgg tgctgttcgg cggaaccgtg ccgctgcaaa gccccgaggc
    15781 gggcaaggtc atgaatactt tattggtgta cgggcgcgac ggcaaacgga cggggctgta
    15841 ccacaaaatg cacctcttcg gcttttccgg tttgggcgaa cgctatgccg aagccgatac
    15901 catccgcgcg ggcggggaag tgccgcactt gtcggcagag ggcgtgccgg tggcggcggg
    15961 catttgttac gatgtccgct ttcccgaatt tttccgccgc cagctgccgt ttgacgtatt
    16021 gatgctgccc gctgcgttta cgcacacgac gggcaaggcg cattgggagc tgctgctgcg
    16081 cgcgcgtgcc gtcgaaaacc aatgttacgt cgtggcggcg gcacagggcg gtttgcacga
    16141 aaacggacgg cgcacgttcg gacacagcat gattgtcgat ccgtggggcg acgtattgga
    16201 cgtattgccc gaaggcgaag gcgttgttac ggcagacatc gatgccaacc gcctgaacag
    16261 cgtccgcaac cgcctgcccg ccttgaaata ccgggttttg gatgccgtct gaaggttcag
    16321 acggcatcgg tgccggggaa tcagcgggct gtaggatggg cttcagcctg ccatttccat
    16381 caatcccaca tttctaccgt tttcatcgaa tccatcgaat ccgccccttt cgacgaccca
    16441 gtcctacgca actgaaccgt cattcccacg gaagtgggaa tctagaatct cgggggtttc
    16501 agtcatttcc gatagattcc cgccgcgtca ggggtctgga ttcccgcctg cgcgggaatg
    16561 acgaatttcg agattgcggt gttgtcggga atgacgaatc catccatacg gaaacctgca
    16621 ccacgtcatt cccacgaaag tgggaatcta gaatctcggg ggtttcagtc atttccgata
    16681 gattcacgcc gcgtcggagg tctagattcc cgcctgcgcg ggaatgacgg atccatccat
    16741 acggaaacct gcatcccgtc attcccacga aagtgggaat ctagaatctc ggggtttcag
    16801 tcatttccga tagattcccg ccgcgtcggg ggtctggatt cccgcctgcg cgggaatgac
    16861 gaatttcgag attacggtgt tgtcggaacg aaactgaatc gtcattccca cgaaagtggg
    16921 aatctagaac gcggggtttg ggcaactgtt tttatccgat aagtttctgt gcggacaggt
    16981 ctggattccc gcctgtgcgg gaatgacgaa tccatccata cggaacctgc accacgtcat
    17041 tcccacgaaa gtgggaatct agaatctcgg ggtttcagtc atttccgata gattcccgcc
    17101 gcgtcagggg gtctggattc ccgcctgcgc gggaatgacg ggtttcaaga ttgcggtatt
    17161 gtcggacgg
//
LOCUS       DAWFIE010000040        16868 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWFIE010000040 DAWFIE010000000
VERSION     DAWFIE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16868
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.040"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(77..730)
                     /locus_tag="WGQ71_001219"
     CDS             complement(77..730)
                     /locus_tag="WGQ71_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ0707231.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWRIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
     gene            complement(762..1274)
                     /gene="coaD"
                     /locus_tag="WGQ71_001220"
     CDS             complement(762..1274)
                     /gene="coaD"
                     /locus_tag="WGQ71_001220"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ0707232.1"
                     /translation="MSPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTIAERRDMLHDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            complement(1403..2074)
                     /locus_tag="WGQ71_001221"
     CDS             complement(1403..2074)
                     /locus_tag="WGQ71_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEZ0707233.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVIANL
                     FLGIPALALTISAGFVLFGSLMIMWQVRTVIDEGEDSHISAALTLFISLYNIFSSLLN
                     ILGDD"
     gene            complement(2231..2497)
                     /locus_tag="WGQ71_001222"
     CDS             complement(2231..2497)
                     /locus_tag="WGQ71_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ0707234.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(2570..3040)
                     /gene="rlmH"
                     /locus_tag="WGQ71_001223"
     CDS             complement(2570..3040)
                     /gene="rlmH"
                     /locus_tag="WGQ71_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ0707235.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVFLTEQLYRAVSILHNHPYHRE"
     gene            complement(3094..3480)
                     /gene="rsfS"
                     /locus_tag="WGQ71_001224"
     CDS             complement(3094..3480)
                     /gene="rsfS"
                     /locus_tag="WGQ71_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ0707236.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            complement(3539..4144)
                     /gene="nadD"
                     /locus_tag="WGQ71_001225"
     CDS             complement(3539..4144)
                     /gene="nadD"
                     /locus_tag="WGQ71_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ0707237.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDTVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSFQDGSIRIL
                     SAPMHNVSSTEIRRNLSAAGVSDGIPPAAARYIREHRLYEK"
     gene            complement(4141..4740)
                     /locus_tag="WGQ71_001226"
     CDS             complement(4141..4740)
                     /locus_tag="WGQ71_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ0707238.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     ELLPNALGLRNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(4769..6310)
                     /locus_tag="WGQ71_001227"
     CDS             complement(4769..6310)
                     /locus_tag="WGQ71_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ0707239.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGGQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITLPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDGGEGGK
                     RTETL"
     gene            6549..7934
                     /locus_tag="WGQ71_001228"
     CDS             6549..7934
                     /locus_tag="WGQ71_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ0707240.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVTAA
                     DFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            7954..8472
                     /locus_tag="WGQ71_001229"
     CDS             7954..8472
                     /locus_tag="WGQ71_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ0707241.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPHAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(8811..9728)
                     /gene="thrB"
                     /locus_tag="WGQ71_001230"
     CDS             complement(8811..9728)
                     /gene="thrB"
                     /locus_tag="WGQ71_001230"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ0707242.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLPR
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWVRTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLG"
     gene            complement(9765..10493)
                     /gene="ubiG"
                     /locus_tag="WGQ71_001231"
     CDS             complement(9765..10493)
                     /gene="ubiG"
                     /locus_tag="WGQ71_001231"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ0707243.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLRGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            10645..11886
                     /locus_tag="WGQ71_001232"
     CDS             10645..11886
                     /locus_tag="WGQ71_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ0707244.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRSWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKLPVLFDTQAPTGTNYWIYVATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            complement(11956..13607)
                     /locus_tag="WGQ71_001233"
                     /pseudo
     CDS             complement(11956..13607)
                     /locus_tag="WGQ71_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            13777..14349
                     /locus_tag="WGQ71_001234"
     CDS             13777..14349
                     /locus_tag="WGQ71_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0707245.1"
                     /translation="MNYKIYDGAKIIKKENLSIGDYSQIDDFVFFNCGEKSILGSFVH
                     ISSFTSIIGGGEFYMDHFSGLSAGCRIITGSDDFMGGGGLTNPTVPSKYTNVKKSSVR
                     IGKHAILGTNVTVLPGVTIGDGVAVGAGAIVRKNLEPWTIYVGVDCKPLKRRSQDKIL
                     ELEKHLVAEFNLDLCPIIPSPTRPNPKETR"
     gene            14346..14909
                     /gene="gmhB"
                     /locus_tag="WGQ71_001235"
     CDS             14346..14909
                     /gene="gmhB"
                     /locus_tag="WGQ71_001235"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ0707246.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTGADGCGCRKP
                     KPGMIEDILERFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            14939..15682
                     /locus_tag="WGQ71_001236"
     CDS             14939..15682
                     /locus_tag="WGQ71_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0707247.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            15679..16371
                     /locus_tag="WGQ71_001237"
     CDS             15679..16371
                     /locus_tag="WGQ71_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0707248.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CHKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPKAKQW
                     LKIHGRELFALG"
BASE COUNT         4015 a         5021 c         4206 g         3626 t
ORIGIN      
        1 atttgcggtc aaccgttttc ttgaaaaaat tttataaaaa tatttatcat attgtttata
       61 aattaattta tatttattaa cctgcttttg ccatataaaa ttcttccagc gcatgagaga
      121 tattttcagt ctgccattcg ttttttaagg gtgcaacaat ttcgatttgt cggttttggt
      181 aggcaaattg tattctccat gcatacagaa acatggtttc ggattctgtt ccgccgtata
      241 agctgtcgcc cagaatcgga ctgcccaaac ttttcatcgc cactctcaat tggtgcgttt
      301 tgcccgtatg cggttccagg atgaacagcc gcagtttttc ggcgatactg atgctgtgga
      361 atcgggtaac ggcgatattt tctgtattgc gcgtcaactt ccacattccg cgtctggatt
      421 tttccattcc gcctttaatc caaccctgct ttttggacgg tttgcggtcg gatagtgcca
      481 aataggtttt tttgatgctt ttgccggcaa actgtccgct aagggcggac gcgctttctt
      541 tgttgagggc aaacagtaaa atgccgctgg tctgtttgtc caatcggtgc agcagccaca
      601 cacgctctac gcccaactgt atggcgagtg ttcgggccag tccggtctcg ccgctgtctt
      661 ggtggacgga tatgccgccc ggtttgttga tggcgacgaa gtcttgatgg cggaacaaaa
      721 tttccaacat atccatatat gccttgcaaa aatagaaggg ttcaattttc gtgttgatgt
      781 tcggcaagga ttttttcgta cacagcttgc ggcacgtagc ggtggatcgt ttccgtccag
      841 ccttccggcc cgaccaagcc tttaaccata gtggacgaca cttcggcgat ttcgcgcggc
      901 ggcataagga atacggtgga tatttcgggt gcgaggtcgc tgttgatatg gcgcatggag
      961 cgttcatatt cataatcgga ggcggaacgg atgccgcgca cgatgaatcc tgcatctacc
     1021 tcacgggcgt aatgcaccaa aaaccggttt tcaaatacat cggttctaac gttaggaaac
     1081 attttagtaa tgtcgtgcag catatcccgc ctttcggcta tcgtgtaggt gctgcgtttg
     1141 tcgggattaa tgccgatggc gacgatgagt tcgtcaaaca tagattgcgc ctgccgtatc
     1201 atccacagat gccccaatgt gggcggatcg aaactgccgg cataaacggc gcggcgtggt
     1261 gtattcggtg acatatgatt ccttccgaca gcatggtcga ccgtatgttg atgaatgtgt
     1321 atccgtattg tatgcccaaa acaaaatgcc gtctgaaata agtgctttca gacggcatgg
     1381 tttgcatggt gtttcgccta catcagtcat cgccgaggat gttgagcagg ctgctgaaga
     1441 tgttgtaaag cgagataaac agtgtcagtg ccgcgctgat gtggctgtct tcgccttcgt
     1501 cgatgacggt gcgtacctgc cacataatca ttaaggaacc gaacaagaca aaaccggcgg
     1561 aaatggtcaa ggcaagtgcg ggaataccca aaaacaggtt ggcaatcacg gcgaccatca
     1621 gaatgaccgc gcctacggtc aggaagcgtc cgagcgcgtt catatcgagc cgggttcggc
     1681 gcgccaaggc ggacatcgtt aaaaagacgg cggcggtcat cgcggcggca atgccgacga
     1741 ttttcgcacc gtcggcaata tggagcgcgt attgcagcac ggggccgatc aatacgccca
     1801 taccgaatgt gaataccatc agcagggtaa cgccggtatt gctgtaacgg tttttctcga
     1861 tgaagtggat cataccgtag aaaaacgcca acacgacggc aaaccctatc cagcgcgaac
     1921 cgaaggcggc gtaaaaattg aaaccggcat tggcggcaag tgccgcgcct gcggcagccg
     1981 gaataaatga aaatccgagc aggcggtagg ttttctgcag gacggtgttt ttagaaaccg
     2041 tatgcgcggt gtagtcgtaa acgtcgtgtt gcatatcatc tgctcctgaa agcgcggttg
     2101 ggaataatgg gggattttaa cattgcccaa tgtcaaaatt tgtccggttg cgtgaagata
     2161 aagttgtccg gcgtatttta aaggccgtct gaagcagttt cggacagcct gtgttcaaaa
     2221 cggaaaaccg ttattgcgga acgtatccct gaacggcatc cgcgccgtcg ccgaagaaat
     2281 actgctccat ctgctgagcc aggtattcgc gcgcgcgcgg atcggcgagg cttaaacggt
     2341 tttcgttaat cagcatcgtt tggtggcgcg tccacgccgc ccacgcttct tgcgatacgt
     2401 tttcaaaaat gcgcttgccc aattcgttgg gaagcggcgg aaatttcatg ccttcggctt
     2461 ctttgttgag cttgacgcag aataccatgc gtgccatagc ggattccttt gctgtgttca
     2521 gaaataacgg ggtgatttta accgattagg gatacggaca aaagccttct tattcccgat
     2581 gatagggatg gttgtgcagg atggaaacgg cgcggtagag ctgctcggtc agaaagacgc
     2641 gcaccatgcc gtgcggcagg gtgaggctgg acaggcgcat catcatgcgt gcctgctgtt
     2701 tgaggcggtc ggtcataccg tccgcgccgc cgatgacgaa gcagacgtgt tcgccgtttt
     2761 gctgccagct tttgaggtgt tccgccagct cgacggaggt cggtgctttg ccgcgttcgt
     2821 caagaacgac gaggaacgcg ccttgcggaa tggcttcaag gatgcgtttt tcttccgccg
     2881 ccataccttg ggcggcattc acgcccgcgc cgcgtttttc gggtttgatt tccttgagcg
     2941 cgtaggcgac gtcgcgtccg aagcgtttgg cgtattcggc aacagcttcg tcaacccagc
     3001 gcggcatttt ggtgccgact gccaaaacgg taatgttcaa tgctttctcc cttacaggaa
     3061 aatgccgtct gaacattcag acggcatcgg gaatcagtct gccgcgtgcc acggcttctg
     3121 cattccggcg tgaaaactcg gtttctcgcc gccccaaagg gtgtcgatgt cgtagaagtc
     3181 gcgcacggca gggagcatga cgtggacaac gaggtcgcct gcatcaacca acgtccattc
     3241 gccgctgtcg ccttcggtgc tgaggatttc aaaaccggct tctttcaaat cgacggcaac
     3301 gttgttggcc agtgctttga cttggcgcgt actgtcgccg ctggcgataa tcattctggc
     3361 aaacagcgaa gttttgtctt gcgtttcgag aacggaaatg tctttggctt tgatgtcttc
     3421 gagggcgttg acggcgaccc cgaccatttt ttgcaggtct tgcagttctt gttcgttcat
     3481 tattttccta acgggatgtt ttcagacggc attatagccg tttcttactg atttgacttt
     3541 atttttcgta cagccggtgt tctcggatat aacgggcggc ggcaggcggg atgccgtctg
     3601 aaacaccggc ggcactcagg ttgcggcgga tttccgttga cgacacatta tgcatcgggg
     3661 cggacaagat gcggatgctg ccgtcctgaa aggacttgcc cagccacgcg tgcagttcgc
     3721 gcggggtttg gtgcaggctg tcgccctgcc tcatggcgac ggcgatattg gtttcgcgca
     3781 cgagcatctg ccattttttc catgtgtgca gcttcatcag gctgtcgctg cccatcagcc
     3841 accaaagttg cgcggatggg aactgctggc ggaagatttg gacggtatca aaagtatagg
     3901 ttgcaccttc tcggacgatg tcgcaatcgc tgacggcaaa acgcgcgtct tctgccgtcg
     3961 ccagttcgac catggcaagg cggtcggcgg cggaagcgga ggctgcgtct ttgtgatacg
     4021 ggccgcctgt cggcaggaaa acaaccgtgt ccaagccgat ttcatcggca aaggcacggg
     4081 cgatatgaag atgtccgttg tgtatcgggt cgaaagtacc gccgaacaat ccgattttct
     4141 tcatgatgtt tccattcctt ccgataagtc catgccgtct gaaacactgc cgtccacaac
     4201 cagcgtcagt ttgccggtaa cgggatggat gaccagtccg tgaacggcga tatggcgcgg
     4261 catcagcgga tggttacgga taaggtccac cgtgtggcgc acgctgtctt cgacgttgtc
     4321 gaaaccggtc agccagccgt cgaggtcgat accggcataa cgcagggttt cgatgcggtc
     4381 ttcgggaatc cggctttccc ggacgcgccc gaggaattct tcggcattca gcccctgcat
     4441 accgcaatcg tgatgggcga tgaccataat ctctctgacc ttcaattcaa acacggcaac
     4501 caacaggctc ctcatcaccg aaccccacgg gtgcgtaacc agcgcgccgg catttttaat
     4561 cagcttggca tcgccgttcc tcaatcccaa agcattgggc agcagctcga taatccgcgc
     4621 atccatacag gacaaaaccg ccagcccgcg ttcggggtat ttgtcggtaa agtatttttc
     4681 atattcgccc gattcgacaa actgccggtt gtgggcaagg atgttatcca actcgctcat
     4741 tttgccgtcc tctgaaaaag ggttcgcatt ataacgtttc cgtccgtttt ccgccttcgc
     4801 cgccgtccaa cagcaggaaa atgcacaccg caaatgccga caacagcaat gtcaacacca
     4861 ttgcccgcgc ataattatcc tcacccgcac gccccaaata ggcataaatc aaagtcgtca
     4921 acgtctgcca ttccggacgc gacaggaaca atgtcgccgc aaattcgccc acacacgtcg
     4981 ccgccgccaa agtcagaccg cgccgcaacg ccggtttcaa gagggggagt gtaatacggc
     5041 atgccgtctg aaagccgttt gcacccaaac ccgccgccgc cctgccgtaa tccggcggca
     5101 gtgcatccca ggctgataaa acatcttttg ccacaaacgg atacgccagc agcgcataca
     5161 tcgccagcag caacggcaac gaagccgtcc accccggata aagcagcagc acgcccgccg
     5221 aaacacaaac cggcgacacc ataaacggca aaaacatcag cccgcgcatc cacgctgacc
     5281 gccgcgccgc cgccgcatac accacaccca aaaccgccgc cgcaaacacc gccgccgccg
     5341 agaagcgcaa agtattccac actgcctgcc acgtttcact ttccattaac acacgccacg
     5401 attcgccggc cgaccacgct ttcacaacaa ttgccaacaa aggaaacagg cagcacacag
     5461 acaacaccgc cgccgcaaac gccagcagca catattcccc gaccgactgc ggcggcgacg
     5521 gcatcacagg ggaaaccgcc ttatccgaaa ccgcgcgcct gccgaaccac gcatacagca
     5581 accctgccgc cgccgttacc cccaacacca gccacaccag caccgaggca accgccatat
     5641 cgagttcaaa catgaccaac tggtaaattt ccacttcgac cgtggcataa cggctgccgc
     5701 ccagcagcaa tgccagcccg aacccggaaa aacaatacag gaagacaagg cacacgccgc
     5761 cggcaagcca cgggcgcaaa acgggcattt caatgtccca aaaccgccgc cacgcccccg
     5821 cgcccaacgt ccgtgccgtc tgaagccgtg ccgcaggcac ttgcacaaac ccctgatacg
     5881 ccgccctgac caacacagga aggttaaaaa acacattgcc gtacaacaac agatacggcg
     5941 tatcctgccc gccgcgccac aacaggccgt ccgccccgaa cagagccagc acgcccacgc
     6001 ccgccaccaa cgtgggcatc acaaaaggca gcatcagcag gcgcagcacc aaagcccgcc
     6061 ccggaaacgc cagccgcgcc agcacccacg cgacaggcac gcccaaaggc agcaccagca
     6121 cacaggttgc cgctgcctga aataccgtcc acgccaaacg tttgagcata taggcatccg
     6181 acagcaccgc gcgccacgcc aaaccgtcat acgccgccac cgcccacaaa ggcgcaacga
     6241 ccattaccgc caaaaaagcc gaaggcagca gggcaaaagc accccatacc gcccaacgcc
     6301 gtccatccat cgccttcccc acttgaaaca ctgatgttgc gattgtaccc aaaagcccca
     6361 cataccgtat atttcaatcc gactacatac cgtatccgcc ttcctcccgc cgtctgaaat
     6421 aaatcgttca aataaacagg aatataactt cagacaaaca acttaccgcc cgatttgtgc
     6481 tatcgttttc gcacaactta aaaaaacctg acaattttgt acttttatta cagagaaagg
     6541 ctttacaaat ggacggctgg acacagacgc tgtccgcgca aaccctgttg ggcatttcgg
     6601 cggcggcaat catcctcatt ctgattttaa tcgtcaaatt ccgcatccac gcgctgctga
     6661 cactggtcat cgtcagcctg ctgacggctt tggcaactgg tttgcccaca ggcagcattg
     6721 tcaacgacgt actggtcaaa aacttcggcg gcacgctcgg cggcgtggcg cttctggtcg
     6781 gcctgggcgc gatgctcgga cgtctagtcg aaacatccgg cggcgcacag tcgctggcgg
     6841 acgcgctgat ccggatgttc ggcgaaaaac gcgcaccgtt cgcgctgggc gttgcctcgc
     6901 tgattttcgg cttcccgatt ttcttcgatg ccggactaat cgtcatgctg cccatcgtgt
     6961 tcgccaccgc acggcgcatg aaacaggacg tactgccctt cgcgcttgcc tccatcggcg
     7021 cattttccgt catgcacgtc ttcctgccgc cccatccggg cccgattgcc gcttccgaat
     7081 tttacggcgc gaacatcggc caagttttga ttttgggtct gccgaccgcc ttcatcacat
     7141 ggtatttcag cggctatatg ctcggcaaag tgttggggcg caccatccat gttcccgttc
     7201 ccgaactgct cagcggcggc acgcaagaca acgacctgcc gaaagaacct gccaaagcag
     7261 gaacggtcgt cgccatcatg ctgattccca tgctgctgat tttcctgaat accggcgtat
     7321 cggccctcat cagcgaaaaa ctcgtaagtg cggacgaaac ctgggttcag acggcaaaaa
     7381 taatcggttc gacaccgatc gcccttctga tttccgtatt ggtcgcactg tttgtcttgg
     7441 gacgcaaacg cggcgaaagc ggcagcgcgt tggaaaaaac cgtggacggc gcactcgccc
     7501 ccgtctgttc cgtgattctg attaccggcg cgggcggtat gttcggcggc gttttgcgcg
     7561 cttccggcat cggcaaggca ctcgccgaca gcatggcgga tttgggcatt cccgtccttt
     7621 tgggctgttt ccttgtcgcc ttggcactgc gtatcgcgca aggttcggca accgtcgccc
     7681 tgaccaccgc cgccgcgctg atggctcctg ccgttaccgc cgccgacttt accgactggc
     7741 agctcgcctg tatcgtattg gcaacggcgg caggttcggt cggttgcagc cacttcaacg
     7801 actccggctt ctggctggtc ggccgcctct tggacatgga cgtaccgacc acgctgaaaa
     7861 cctggacggt caaccaaacc ctcatcgcac tcatcggctt tgccttgtcc gcactgctgt
     7921 tcgccatcgt ctgacagacg gaaaggatag taaatgacta cgcattttgt cgttatgggc
     7981 gtatgcggct gcggcaagac caccgccgcg ctgtccctgc agaaacacct cggtcaatgt
     8041 ccccatgccg aaggcgacga gttccacacc caagccaacc gcgacaagat gggcgcgggt
     8101 attccgctga ccgatgaaga ccgctatccg tggttgggca atctgcgcga ctggatgacg
     8161 caacaggcgc aaaacggtgc gaaccacacc atcgtaacct gttccgccct caaacgcggc
     8221 taccgcgaca ttctgcgcgg agccgaaggc aaagctgctt tcatccacct cagtccgccg
     8281 caagacatca acctcgagcg catgatgtcg cgcaaaggac attacatgaa agcagggatg
     8341 ctcgattcgc aactggaaat cctcgaggaa ctgggcgaag gcgaatacgg ggtcaaaatc
     8401 gccaaccccg gcacgcccga agcggtcgaa gccgatattc tgaactgggt tgcctcggaa
     8461 aacctgcttt gaagcaatat gccgtctgaa gcccgacaca ggatgggttt cagacggcat
     8521 aaacatcggg aacagaatgg attacattga tttatagtgg attaacaaaa accagtacag
     8581 cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctgaagca ccgagtgaat
     8641 cggttccgta ctatctgtac tgtcttcggc ttggtcgcct tgtcctgatt tttgttaatc
     8701 cactataaaa tggaaaatac ccggctatcg tctcattttc gttttaatca gccataaaaa
     8761 tgccgtctga aaccctttca gacggcattt ctgtcaaacg ccggacgcac tcaacccaaa
     8821 ctcaacagca ggttgcggaa cgcgttcggg tctttgataa acgtcatctc gcccgcctgc
     8881 ggaaaatgaa aatccaacag gcgcgacacc caaaaacgga tgcagccggc gcgttgggcg
     8941 gtcgggaaat acgccttttc ttcggcactc aaggggcgca cgccctcata accgccgata
     9001 aacgcctttt tcaacgcctc atccaactta ttgtccgccg tccttaccca atcgttgacc
     9061 gcaatcgcca agtcatacat aaaattgccc cggcaggcat aatagaaatc gatgaaaccc
     9121 gatacctgac cgccgtcaag caacacatta tccttgaaca aatcggcgtg gatgatgccc
     9181 gaaggcagat ggttgccgag attgtccttc agcgcatcga tttcggaaca cagcagcgcg
     9241 gcatcgtctc gcggcaggac gggcagcagt cgggcgcacg cctctgtcca ccacgcatca
     9301 taacgcgggt tttccatttc caaagggaaa tcggcggcgg caaggtgcat tttcgccaac
     9361 atcgcgccgg tatggaaaca ctgttccgcc gtcggcagcg cggtgtccga acctttcagg
     9421 caggcaacca ggcatgcggg cttgcccgcc aaaacggaat caagccggcc gtctttgcgc
     9481 gcaaccggcg cggcaaccgc cacgcccttc atactcaaat gccggttaag ctccagaaaa
     9541 aacggcagct cttcctgttt caacacttca aacacggtca gcacataacg tcccgaagtc
     9601 gtcgtcagaa aataattgct gttggtaatc ccctgcgcga tgccctgcag ggaaacaaat
     9661 tcccccaaat cgtaaccgct caggaagccg cgcatttcat catcggaaac actggtatag
     9721 acagacatat caactcactt gctcaaaaac gggcaaaccc gccgtcagaa cgccggacgg
     9781 caggcaaaca tataattcac atcggtcgaa tcgcacaggg cataagtttg cgacaacaca
     9841 tggtaagtca tacccttcgt atccgccaca tccaagcctg cctgacggca catccgcgcc
     9901 agctcggcag gcgcgatgaa ttttttccag tcgtgcgtgc ctttggggac aaacttcaac
     9961 agatattccg ccgccacaat cagatgcagg tacgatttcg ggtttttatt gatggtggaa
    10021 aaaaacacca tgccgtccgg tttgaccaga ttggcacaag cacgcacgat ggcggcggga
    10081 tcggggacgt gttccatcat ttccatgcac gttaccacat cgaacgagtg cggttccgcc
    10141 tcggcaaggt cttccacgcg gatacattcg tattcgatat cggcgacatt gttcaaagcc
    10201 gcgtgcaggc gggcggtttc caacgactgc tccgccatgt cgatgccctt tacaaacgcc
    10261 gcgccgcgcc gcgccatact ttccgccaag atgccgccgc cgcagcccac gtccaaaacc
    10321 cgtttgccgc gcaaatccgc gtgtccgtcg atataatcca gccgcagcgg attgatgtcg
    10381 tgcaaggttt tgaactcgcc cgacttgtcc caccatttgt cggcaatccg gctgaatttg
    10441 gcgatttccc cctcatcgac attatatttt ttgtcggaca ttttccctcc catctgacga
    10501 accgcccact ccaaaaccca agattatact atggaacgca atgccgtctg aaagcctttt
    10561 cgggcggcgc ggcgcaaata ccttacaaat ccttacactt tacggcataa tggcggctcg
    10621 ctttttctgg cagaaagaca aaatatgccc accaaaaccc cttcactgtt cggcggcgcg
    10681 atgattatcg ccggcacggt catcggcgca ggtatgctcg ccaacccgac cgccacatcc
    10741 ggcgtatggt ttaccggctc gctggccgtg ttgctgtaca cctggttttc catgctctcc
    10801 agcggcctga tgattttgga agtcaacacc cactaccccc acggcgcgag cttcgacacc
    10861 atggttaaag acctgctcgg acggagctgg aacatcatca acggcatcgc cgtcgccttc
    10921 gttttatacc tgcttactta cgcttatatc ttcgtcggcg gcgacctgac cgccaaaggc
    10981 ttaggcagcg cggcaggcgg caatgtttca ctcaccgtcg gacaactcgt cttcttcggc
    11041 attctcgcct tttgcgtatg ggcatccgca cgcttggtcg accgattcac cagcgtcctc
    11101 atcggcggca tggtattaac ctttatttgg gcaaccggcg gcctgattgc cgatgccaaa
    11161 ctgcccgtcc tcttcgacac ccaagcccct accggcacca actactggat ttatgtcgcc
    11221 accgccctgc ccgtctgcct tgcgtcattc ggtttccacg gcaacgtctc cagcctgctc
    11281 aaatacttta aaggcgacgc gcccaaagtg gctaaatcca tctggacggg cacactgatt
    11341 gcgctggtaa tttacgtcct ctggcaaacc gccatccaag gcaacctgcc gcgcaacgag
    11401 ttcgcccccg tgattgccgc cgaagggcaa gtctccgtcc tgattgaaac cctgtccaaa
    11461 ttcgcccaaa ccggcaatat ggacaaaata ttgtccctgt tttcctatat ggcgatcgcc
    11521 acctcgtttt taggcgtaac gctcggactc ttcgactaca tcgccgacat cttcaaatgg
    11581 aacgacagcg tgtccggccg caccaaaacc gccgcgctga ccttcctgcc gcctctaatt
    11641 tcctgcctgc tcttccccac cggctttgtt accgccatcg gctacgtcgg cctggcggca
    11701 accgtctgga caggcatcat ccccgccatg ctgctctacc gttcgcgcaa aaaattcggc
    11761 gcaggcaaaa cctataaagt ttacggcggc ttgtggctga tggtttgggt cttccttttc
    11821 ggcatcgtca acatcgccgc acaggtattg agccaaatgg aactcgtccc cgtatttaaa
    11881 ggataaaggc aaaatgccgt ctgaacgagt tttcagacgg cattttcgca accgttgcag
    11941 ccatgcagca aaaagctaac gggcaatttt caaaacgtcg tacagatagt tgcggtaatt
    12001 tccgaccaac tcccagttcg ggcagtcttc caaccctccg ccgcacaaac cgcgcatcgc
    12061 catattcgca cgcatgaagc cgtctttggc ttccagacca gccgcctcca tcagcacgcc
    12121 gcctgcaaag gcaaggtcga ggaaattctg ccgctgtacg aatccgccgg caatattgct
    12181 cctgacggca aactgcgtta cataatccgg ctgcgcgtaa tcccatttct tcctgacgga
    12241 cacgccctca aacggtacct gatgatcgcc gaaataaccg aaaacaaagg gtttgccgcg
    12301 ttcgtgcaga taacggtcga aactttccac cgctttgtcc aaatcggcaa tgcgcccgat
    12361 gtagtcgtta agcgcggata cggttttcgc attcaaatcg ggcgcatcca aatcaaacac
    12421 attgtcggta tccgtccgat acggcccgtg ctccttcatg gtcagcacat atacgaacat
    12481 cggctgccgc acgttttcca aatcgggatg gcgtttttcc aaaatcatcc gcgcatactg
    12541 catcatttcc tcactggaaa tatgccacag gtttttgccc atcggcgcgg gatagcccaa
    12601 atcttgcggc tggaacatca gattaaagcc gaaatggtca tatgctgcct tggcattgta
    12661 gttgcccttg gtaaatggcg agagtgccac gcaaaaataa ccgtgttcgc gcaggttgcg
    12721 gacaaaaccg gtctgcaaat gcggtacgac cgagtaaaac acgccgcttg ccaacgcgcc
    12781 gaaatccgtg gacggaacac ccgccaaaaa cgcaaattcg gacttccacg ttgcgccgcc
    12841 gaaagtatgc acgcgcaaag gtgaggaaaa tacggtatct tcctgccgtc cgaacatttt
    12901 caaatcggga atcttggcgg cggcaaagtc gaaacagtgc ggatcgagcg tcgattccat
    12961 cagggtaacg acaatatcgg gtttttcatc cgacatgccg cacggccggg tttcagacgg
    13021 catctgcctg gcaaacgctt ccccgtcgcc ctcgaacacg ggtacttgga aaaacaccgc
    13081 ccgacaggac atcggcaggt tcaaaacacg tcgcgcccgt cgtccggcag cgaatccagc
    13141 cacgtcttta ccgcgcccgg atgtttggaa aaatgccgca tcgacacgaa cgccgccgca
    13201 aacaaaaccg cgcctgccca ccgccacggc acatccaaag aatccgcacc gctccagccg
    13261 aaaaccgcat atgccgccaa agccagcagc cccgccatac cgataaccgc ttccttataa
    13321 tggaacagcg tttcccaatt ccgccagtcg gcaatcagca aaaagtcgga aatcaacagc
    13381 ggctgtttgt aataatggat tttcagcctg tggaacagta tcagcaccac aaaccagacc
    13441 gaagcgaaat tcaaggcacg ttgccactga gccgtcaaca tcagcatacc gcccgccaaa
    13501 aagacaaaca gcgcggatgc gaaaaaatac gtccagcggt agtgcgacct gataatcagc
    13561 accaacgccg ccgttacaaa caaaaacagg aaaacatagg caaccatccc gccccactcc
    13621 tcaaaacggc atacctgagc cgttgcgaat tattatcgaa agcgtgcatt ataatgcagc
    13681 acaggcgcat cactgccgat atttcagtat aattattcga gatggaatca ctgaaaatgg
    13741 tgaagttaca atgagccctt ttagaggaac taaaaagtga attataaaat atacgatggt
    13801 gcaaagatta ttaaaaaaga aaatttatca attggggatt attctcaaat tgatgatttt
    13861 gtatttttta attgtggaga gaaaagtatt ttaggaagtt ttgttcatat atcatcattt
    13921 accagtatta ttggtggtgg agaattttat atggatcatt tttcaggatt aagcgcaggg
    13981 tgtagaatta ttaccggtag cgatgatttt atgggggggg gggggttaac caatccaaca
    14041 gtcccatcta aatatactaa tgttaaaaaa tctagtgtta gaattggaaa acatgcaatt
    14101 ttaggaacaa atgtaacagt attaccaggc gttactattg gtgatggggt tgctgttgga
    14161 gcaggggcta tagtgagaaa aaatcttgag ccgtggacta tttatgtcgg ggtagattgt
    14221 aaaccactga agcgcagatc acaagataaa atattggagt tagagaaaca tcttgtagca
    14281 gaatttaatc tagatttatg tcctataatc ccatccccga cacgccccaa cccaaaggaa
    14341 acgcgatgaa actcatcatt ctcgaccgcg acggcgtcat caatcaggac cgcgatgact
    14401 tcgtcaaatc cgttgacgag tggatacctg tcgaaggcag catggatgcg gtggcatttc
    14461 tgacgcaggc aggctacacc gtcgccgttg ccaccaacca atccggcatc gggcgcaaat
    14521 attttaccgt tcaaaacctc accgaaatgc atgccaaaat gcaccgcctc gtccgtcagg
    14581 caggcggcga aatcaacggc atctggttct gccctcatac cggcgccgac ggctgcggct
    14641 gccgcaagcc caaaccgggt atgattgaag acatcctcga acgcttcaac gcccaagctt
    14701 cggaaacttg gctggtcggc gacagcctgc gcgatttgca ggcaatcgat gccgtcggcg
    14761 gcaaacccgc gctggttctg accggaaaag gcaaaaaaac gctctcccaa cacggacacg
    14821 aattgcccga acacacacag gttttcgata ccttgctcga tttctcacaa tacatcatgc
    14881 aggaaaacgc cgcaccgcaa gccgactgaa cataccgcat ttccgacaag gcaaaaccat
    14941 gctcatcatc cgcaacctga tttactggct gatactctgt tccaccctga ttttcctctt
    15001 tccctttatg ctgctcgcct cgcctttccg agacggggcg cacaagatgg cgcgggtctg
    15061 ggtcaaaatc ctcaacctct cgctcaaaca catcgtcggg ctcaaatacc gcatcatcgg
    15121 cgcggaaaac atccccgacc gccccgccgt catctgcgcc aaacaccaaa gcggctggga
    15181 aacgctcgcc cttcaggaca tttttccgcc gcaggtttac gttgccaaac gcgagttgtt
    15241 caaaatcccc tttttcggct ggggcttgaa actggtcaaa accataggca tagaccgcaa
    15301 caaccgccgc gaagccaacg agcagctcat aaaacagggg ttggcgcgca aaaacgaagg
    15361 ctattggatt accattttcc ccgaaggcac gcgccttgca cccggaaagc gcggcaaata
    15421 caaactcggc ggcgcgcgca tggcgaaaat gtttgagatg gacatcgtcc ccgtcgccct
    15481 caacagcggc gaattttggc cgaaaaactc ctttctgaaa tatccggggg aaatcaccgt
    15541 cgtcatctgt ccgaccatcc cgcacgcaag cggcagcgaa gccgaattga tgggaaaatg
    15601 cgaacacctc atcgaaacgc agcagccgct catttccggc gcaggcccgt ttgccgccaa
    15661 aatgccgtct gaaaccgcat gaccgccttt gtccacaccc tttcagacgg catggaactg
    15721 accgtcgaaa tcaagcgccg tgccaagaaa aacctgatta tccgccccgc cggcacacat
    15781 accgtccgca tcagcgtccc accctgcttc tccgtctccg ctctaaaccg ctggctgtat
    15841 gaaaacgaag ccgtcctgcg gcaaacactg gcgaaaacac cgccgccgca aactgccgaa
    15901 aaccggctgc ccgaatccat cctcttccac ggcagacagc ttgcccttac cgcccatcaa
    15961 gacacgcaaa tcctgctgat gccgtctgaa atccgtgttc ccgaaggcgc acccgaaaaa
    16021 cagcttgcgc tgctgcggga ctttttggaa cggcaggcgc acagttacct gattccccgc
    16081 ctcgaacgcc acgcccgcac cacacaactg ttccccgcct cctcctcgct aacctctgcc
    16141 aaaaccttct ggggcgtgtg ccacaaaacc acaggcatac gcttcaactg gcggctggtc
    16201 ggcgcaccgg aatacgttgc cgactatgtc tgcatacacg aactctgcca cctcgcccat
    16261 ccagaccaca gccccgcctt ttgggaactg acccgccgct tcgcccccta cacgcccaaa
    16321 gcgaaacagt ggctcaaaat ccacggcagg gaacttttcg ccttaggctg acgcggcccg
    16381 ggcaggcatc cgccaattca gacctcctcc caaacggaca tccggaaagc agcatcacag
    16441 ccggcgacac ataccgctgc ctccgctttc agacggcatc cgtgccggaa caggcacgcg
    16501 cccgcccgat tcaaaccgcg atgacgcttt gccgccggtt cggggcagga tggcggcaca
    16561 cgcgccgtct gccgcgtttc atttcacacc gctcttccga aacccgaaac ccgcccggtc
    16621 cgacgtgcgg tatgaaacgc ttaagctgac gcgaagtctt ttactgattt gcccgcgaaa
    16681 atgccgtctg aaaggttttc ggacggcatt tttttgcgtt tcccaggatg gcggcggatt
    16741 cgtaaaaggc ggtcagggtg gattgtagga tgggttgaga cctgccgaat ccgccgcatc
    16801 tgccaaatct accgccgtca ttcctacgaa agtgggaatc tagaacgcgg ggttaagaaa
    16861 acctgcat
//
LOCUS       DAWFIE010000041        16844 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWFIE010000041 DAWFIE010000000
VERSION     DAWFIE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16844
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.041"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            606..5966
                     /gene="iga"
                     /locus_tag="WGQ71_001238"
                     /pseudo
     CDS             606..5966
                     /gene="iga"
                     /locus_tag="WGQ71_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050177927.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
     gene            complement(6145..7347)
                     /gene="trpB"
                     /locus_tag="WGQ71_001239"
     CDS             complement(6145..7347)
                     /gene="trpB"
                     /locus_tag="WGQ71_001239"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ0707249.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGSPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(7430..8119)
                     /locus_tag="WGQ71_001240"
     CDS             complement(7430..8119)
                     /locus_tag="WGQ71_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ0707250.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDPVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            8316..9095
                     /gene="rsmA"
                     /locus_tag="WGQ71_001241"
     CDS             8316..9095
                     /gene="rsmA"
                     /locus_tag="WGQ71_001241"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ0707251.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIEGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     ELLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELATDEDLQAAGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            9268..9996
                     /locus_tag="WGQ71_001242"
     CDS             9268..9996
                     /locus_tag="WGQ71_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0707252.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            10069..10428
                     /locus_tag="WGQ71_001243"
     CDS             10069..10428
                     /locus_tag="WGQ71_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707253.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            10433..10879
                     /locus_tag="WGQ71_001244"
     CDS             10433..10879
                     /locus_tag="WGQ71_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ0707254.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRHCITHDMADPESYRRLKVWVLWRQPKKTAETDTPS"
     gene            10911..12185
                     /gene="folC"
                     /locus_tag="WGQ71_001245"
     CDS             10911..12185
                     /gene="folC"
                     /locus_tag="WGQ71_001245"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ0707255.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DFDHQAFLGDTVEQVGFEKAGVFRGGKPAICGQNPAPASLLSHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFRPQHSDSPARNRNSLPFPALRGAYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            12198..13196
                     /gene="ftsN"
                     /locus_tag="WGQ71_001246"
     CDS             12198..13196
                     /gene="ftsN"
                     /locus_tag="WGQ71_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0707256.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            13189..13686
                     /locus_tag="WGQ71_001247"
     CDS             13189..13686
                     /locus_tag="WGQ71_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ0707257.1"
                     /translation="MNNLPVADLLVFAIIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFILAYLIQKILRSLLTQAVSSVGLGFANR
                     ILGGVFGALKGVLIVTLLIMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHADDSDG
                     ALEDD"
     gene            13998..15542
                     /gene="purF"
                     /locus_tag="WGQ71_001248"
     CDS             13998..15542
                     /gene="purF"
                     /locus_tag="WGQ71_001248"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ0707258.1"
                     /translation="MCGVLGLVSHAPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHINTGSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMVAFRDPYGIRPLALGSQTDESGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFDGTMIARQCSDKAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDDIDVVMPIPDTSRPSAMELAVYLKKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            15642..16133
                     /gene="greB"
                     /locus_tag="WGQ71_001249"
     CDS             15642..16133
                     /gene="greB"
                     /locus_tag="WGQ71_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ0707259.1"
                     /translation="MSTETKNYITPAGWQALKDELYRLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            16192..16818
                     /locus_tag="WGQ71_001250"
     CDS             16192..16818
                     /locus_tag="WGQ71_001250"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ0707260.1"
                     /translation="MRKIRTKICGITTLEDAAAAAAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT         4730 a         4567 c         4120 g         3427 t
ORIGIN      
        1 cagagcggtt tctgttgctc ccgataaatt cctaaaactc aaaatttcat cattcctaca
       61 aaaacagaaa accaaaatca gaaacctaaa atcccgtcat tcccacgaaa gtgggaatcc
      121 agaatctcgg actttcagat aatctttgaa tattgctgtt gttctaaggt ctggattccc
      181 gcctgcgcgg gaatgacgaa tccatccgta cggaaacctg caccgcgtca ttcccacgaa
      241 cccacatccc gtcattacca cgaaagcggg aatctaggac gcagggttaa gaaaacctac
      301 atcccgtcat tccctcaaaa acagaaaacc aaaatcagaa acctaaaatc ccgtcattcc
      361 cgcacaagcg ggaatccaaa aacaaaaagc cgcagaaatt taacggaaga accgaacccc
      421 gaaaatcggc ttcccacctt cgcctgaata acagaacaaa ggaagcggaa ataaaaacat
      481 cgggattctt tttttaaatt ggcaataaaa caccaaaatg aataattcac acaacaaaaa
      541 atagtcaaca ccgccaaacc ggcgtaaaat acaaaacatt atcaccctta aaacggtaaa
      601 accttatgaa aaccaaacgt tttaaaatta acgccatatc cttatccatc cttcttgcct
      661 atgcccttac gccatactca gaagcggcat tggtcagaga cgatgtcgat tatcaaatat
      721 tccgtgactt tgcagaaaac aaaggcaaat tttttgtcgg cgcaaccgat ttatcagtga
      781 aaaacaaaca aggtcaaaac atcggcaacg cattatccaa cgtaccgatg attgatttta
      841 gcgttgcaga tgtcaacaga cgtacattaa ccgtcatcga tccccagtat gccgtcagcg
      901 tcaaacatgt aaaaggagac gaaatctctt attacgggca tcacaatggg cacttagatg
      961 ttagcaatga tgaaaacgaa tatcgttcag ttgcacaaaa tgactacgaa ccaaataaaa
     1021 attggcatca cggtaatcaa ggtcgtcttg aagactacaa catggcacgc cttaataaat
     1081 tcgtaacaga agtcgcacct attgcaccaa ccagtgcagg cggcggcgtt gaaacctata
     1141 aagataaaaa ccgtttttct gagtttgtgc gagttggagc aggtacgcaa tttgaatata
     1201 acagccgcta caatatgaca gagctttcac gagcttatcg ttatgccatt gcaggtacgc
     1261 cttatcaaga cgtgaatgtc acatcgaatc taaatcaaga aggcttgatt ggcttcggtg
     1321 ataattcaaa acatcattcc ccagaaaaac tcaaagaagt tttatctcaa aacgcattga
     1381 ctaactatgc tgtgttaggc gatagcggtt caccattatt tgcttatgat aaacaagaaa
     1441 aacgctgggt ctttttaggt gcttacgatt attgggcagg ctatcaaaaa aactcttggc
     1501 aagaatggaa tatctataaa aaagaatttg cagataaaat caaacaacgc gataacgccg
     1561 gcaccatcaa aggtaatgga gaacatcatt ggaacattac ttttggcaca aacagcaaga
     1621 ttggttctac agcggtaagg cttgccggca atgaaagggg tgcaaacaac ggacagaatg
     1681 ttacctttga aaacaacggc actttggtat tggatcaaaa catcaaccaa ggcgcgggcg
     1741 gtctgttttt caaaggcgat tacacagtca aaggtgcaaa taatgacatc acttggctag
     1801 gtgcggggat tgatgttgcc gacggcaaaa aagtcgttta gcaagtcaaa aatccgaaag
     1861 gcgataaatt agcgaaaatc ggcaaaggca ctttggaagt aaacggtaca ggcgtaaatc
     1921 aaggcgaatt aaaagtcggc gacggtacgg ttattctgaa tcaacaagcc gatgccgaca
     1981 aaaaagtcca ggctttctcc caagtcggca ttgtgagcgg gcgcggtaca ttggtattaa
     2041 atagttcaaa tcagattaat cccgataacc tatatttcgg tttccgtggc ggtcgtttgg
     2101 atgccaatgg caatgacttg acttttgaac acatccgcaa cgtggatgaa ggtgcgcgca
     2161 ttgtcaacca caacacaggt cacgcctcca caatcacatt aaccggcaaa tcattaatta
     2221 ccgctcctca aaatctttct gtttatgaaa tccgaaatga ttacgatgat gacgattatt
     2281 atggctatta cagctataga aaacccattc cgcaaggcaa agatctttat tacaaaaact
     2341 atcgttatta cgccctaaaa tccggcggca gcgtgaacgc accgatgccc gaaaacggac
     2401 aaacggaaaa taacgactgg attttaatgg gcagcacaca agaagaagcc aagaaaaacg
     2461 cgatgaacca caaaaacaat cagcgtatta gcggttttag cggtttcttt ggcgaagaaa
     2521 acggaaaagg acataacggc gcattaaacc ttgatttcaa cggcaaaagc gcgcaaaacc
     2581 gtttcttgtt aacaggcggc acgaatttaa acggaaaaat aagcgtaact caaggcaatg
     2641 tcttgttatc aggtcgtcca acaccacacg caagagattt tgtgaacaaa tcttcagccc
     2701 aaaaagacgc acatttctcc aaaaacaatg aagtcgtatt tgaagacgac tggataaacc
     2761 gcacattcaa agccacagaa attgcggtta atcaatccgc atcattctct tccggcagaa
     2821 atgtatccga catcaccgcc aacattaccg caacagacaa cgccaaagta aatttgggtt
     2881 acaaaaacgg cgatgaggtt tgcgtgcgct cggactatac cggttacgtt acctgcaaca
     2941 cagacaactt atccgataag gctttaaaca gctttggagc gacacagatt aacggcaatg
     3001 tgaatttgag tcagaatgcg gcattggctt tgggcaaggc tgcattatgg gggcaaattc
     3061 aagggcaagg aaacagccgt gtcagcctaa accaacatag caaatggcat ttgaccggcg
     3121 acagccaagt gcacaatctg tcattggcgg atagccatat tcatttgaac aacgcttccg
     3181 acgcgcaaag tgcaaataaa taccacacgc tcaaaatcaa tcatttatcc ggtaacgggc
     3241 attttcatta tctgacgcac ctggcgaaaa atcttgggga taaagtgctt gtgaaggaat
     3301 ccgcatccgg tcattatcag ctccatgttc aagataaaac aggcgaaccc aatcaggaag
     3361 ggctgaatct ctttgatgca tcatccgtac aagaccgctc ccgcctttct gtttccttgg
     3421 cgaataacca tgtagattta ggcgcattgc gttatacaat caaaacagaa aacggtatta
     3481 cccgattgta caatccttat gccgagaacc gccgccgagt caaaccgacc ccgtctcctg
     3541 ccacaaacac ggcttctcaa gcacaaaagg caacacaaac ggacggtgca caaattgcca
     3601 agcctcaaaa tatcgtcgtc gcaccaccta gcccgcaggc aaatcaagcc gaagaagcaa
     3661 aacgccaaca agcaaaagcg gagcaagtga agcgtcagca agcagaagca gagcgtaaat
     3721 cggcagagtt agcaaaacaa aaagcagaag ccgaacgcga agcaagagag ctggcgaccc
     3781 gccaaaaagc agaacaagaa cgcagcagcg ccgaacttgc acgtcgacat gaaaaagaac
     3841 gcgaagcggc agagttgtcg gcaaaacaaa aagtcgaagc cgaacgtgag gctcaagcat
     3901 tggcggtacg ccgaaaagca gaagccgaag aggcaaaacg ccaagctgca gagcttgcac
     3961 gccggcatga aaaagaacgc gaagcggcag agttgtcggc aaaacaaagg gtcggggaag
     4021 aggaacgccg tcaaacagcc caatcccagc cgcaacgccg caaacgccgt gccgcaccgc
     4081 aggattatat ggcagcttcc caagaccgtc ctaaacgccg cggacaccgt tcggtacagc
     4141 aaaacaatgt ggaaattgcc caagcccaag cagaacttgc gcgccgacag caggaagaac
     4201 gcaaagcggc agagttgttg gcaaaacaaa gggcagaagc cgaacgtgag gctcaagcat
     4261 tggctgcgcg ccgaaaagca gaagccgaag aggcaaaacg ccaagctgcc gaacttgcac
     4321 atcgccagga agcagaacgc aaagcggcag aattgtcggc aaaccaaaaa gccacagctg
     4381 aagcccaagc attggcggca cgccaacaaa aagcccttgc gcgtcaacag gaagaagcac
     4441 gaaaagctgc agagttagcc gtcaaacaaa aagcggaaac cgaacgcaaa accgccgaac
     4501 ttgccaagca aagggcagca gccgaagcag caaaacgcca gcaagaagct cgccagaccg
     4561 ccgaacttgc acgtcgtcaa gaagcggaac gccaagcagc agagttgtcg gcaaaacaaa
     4621 aagccgaaac agatcgagaa gcggcagaat cggcgaaacg aaaagctgag gaagaagagc
     4681 accgtcaagc agcccaatcc cagccgcaac gccgaaaacg ccgtgccgca cctcaggatt
     4741 atatggcagc ttcccaaaat cgtccgaaac gtcgcggacg cagatctact ctgccggcac
     4801 cgccctcgcc atcatttgat tcgtcagctt acgcagcacc cagggccttg cataatccgg
     4861 actggtatga aaatgactat gaagaaatcc ccttggatgc gttggaagat gaaaatgtat
     4921 ctgaatcagt tgacacatca gacaaacagc ctcaagacaa tacggaactt catgaaaaag
     4981 ttgagacagt gagtttgcaa ccaagagccg cgcagccgcg agctcaagcc gccgcgcagc
     5041 cgcaagccca agccgccgcg cagccgcaag cccaagccgt cgcgcaagcc gatgcagtca
     5101 gcaccaatac caactcggct ttatctgacg caatggcaag cacgcaatct atcttgttgg
     5161 atacaggtgc ttcattaaca cggcacattg cacaaaaatc acgtgctgat gccgaaaaaa
     5221 acagcgtttg gatgtcgaac atcggttatg gccgtgatta tgcttccgca caatatcgcc
     5281 ggtttagttc gaaacgcacg caaacacaaa tcggcattga ccgcagcttg tccgaaaata
     5341 tgcagatagg cggcgtattg acttactctg acagtcagca tacttttgat caggcgagcg
     5401 gcaaaaatac ttttgtgcaa gccaaccttt atggtaagta ttatttaaat gatgcttggt
     5461 atgtggccgg cgatattggt gcaggcagct tgagaagccg gttacaaaca cagcaaaaag
     5521 caaactttaa ccgaacaagt atccaaaccg gccttacttt gggcaataca ctgaaaatca
     5581 atcaattcga gattgtcccc agtgcgggga tccgttacag ccgcctgtca tctgcagatt
     5641 acaagttggg taacgacagt gttaaagtaa gttctatgtc agtgaaaaca ctgacggccg
     5701 gactggattt tgcttatcgg tttaaagtcg gcaaccttac cgtaaaaccc ttgttatctg
     5761 cagcttactt tgccaattat ggcaaaggcg gcgtgaatgt gggcggtaat tccttcgcct
     5821 ataaagcaga taatcaacag aaatattcag caggtgccgc gttactgtac cgtaatgtta
     5881 cattaaacgt aaatggcagt attacaaaag gaaaacaatt ggaaaaacaa aaatccggac
     5941 aaattaaaat acagattcgt ttctaaaata ctaaattcat agcaaaataa aatgccgtct
     6001 gaactcaggc ttcagacggc atttttatag tggattaaca aaaatcagga caaggcgacg
     6061 aagccgcaga cagtacagat agtacggcaa ggcgaggcaa cgccgtactg gtttttgtta
     6121 atccactata tcagacggac gaggttacag tttaatccct ttgagcttcg cgacggtatt
     6181 gatgtctttg tcgccacgac cggataggtt gaccagaatc acttggtctt tgcccatttt
     6241 cggcgcgttt ttcaccgccc aagcaacggc gtggctggat tcgagcgcgg ggatgattcc
     6301 ctcgaagcgg cagagcaaat caaaggcttc gagtgcttcg tcgtctttag cgacggtgta
     6361 ttcgacgcgc ttgatgtcgt gcagatggct gtgttccggg ccgatgccgg ggtaatccaa
     6421 gcctgcggaa acagagtgtg tacccaagac ttgaccgttt tcgtcctgca tcagatagct
     6481 gcggaaaccg tgcaatacgc caatcggtga gcccgaagta atcggcgcgg cgtgatcggg
     6541 ggtgttcacg cccaaaccgc cagcctccac gccgacgagg cgcacgtttt cttcgccgat
     6601 atacgggtgg aacaaaccga tggcgttcga tccgccgccc acgcaggcaa cggcaacgtc
     6661 gggctgtctg ccgatggctt cctgcatctg cgctttagct tcgttgccaa tcacgcattg
     6721 gaaatcgcgc accatttcgg gatacggcgc ggggccggcg gcggtaccga tgatgtagaa
     6781 cgtgtcgtcc acgcgggcga cccattcgcg catggcttcg ttcatcgcgt ctttcagcgt
     6841 gcggctgccg ctttctacac cgaccacgtt cgcacccaat aatttcatac ggaacacgtt
     6901 gggcatttgg cgttggatgt cgtccgcgcc catatacacg tcgcaagtca taccgaagcg
     6961 tgcggcaacg gtggcactcg ccacgccgtg ctgacccgcg ccggtttcgg cgatgacgcg
     7021 ttttttaccc atgcgttttg ccaacagtgc ctgaccgatg gtgttgttga ctttgtgcgc
     7081 gccggtgtgg ttcaagtctt cgcgcttcaa ccagatttgc gcgccgccca gatgttcgga
     7141 caaccgcgcg gcgtggtaaa cggggctggg cctgccgaca taatgtttca aatcatggcg
     7201 gaacgcttcc caaaattcag gatcgttttt cgctgcctta taggcatccg ccagctcttg
     7261 caaggcggga atcagggttt cggagacata aagcccgccg tgttcgccga aaaagccctt
     7321 ctcgtcgggc gcatggtagt tcttcatcgg attcttcctt gtgcttcaaa ttttcagacg
     7381 gcatttccgt ttttgcaaac atgccgtctg aacgggaaat tgtcggcaat tataactgtt
     7441 tttcacacaa gcccgccatt tcaaaaaacg cccgatacgc cgccgctttt ttcgccaaac
     7501 tcaaaggata ggcgcgcgaa gtcgaaggca ggcgcgtcag cgtcaaatcc cgtccggcaa
     7561 acggaaacgg cacggtttcg cccgttttcg gcattttgat accgccgccc tgaatatcga
     7621 gcaggatttc cgtcgccttg ccgcctgtcg tacaaatatg gcggcactcg ggtatttttg
     7681 ccaacaccgc cgccaaatcg acggtttcaa ctacctttaa aaacttgtcg gacgcattgc
     7741 cgtgctgacg taccgccttc aaaacggtcg gacaggacgc aatcccccgt tcgtgcaaaa
     7801 acgccttgat tttctcggca tcaaacgctt tttcagacaa cctttggaaa tgcgccgcat
     7861 cattaaaaaa caccagccca taaacgcgcc acatatcgtt ttggaaattc ggataatgaa
     7921 actgcatcgc gcgtttgtct tccttgggcg gaaacgtccc catcatcatg acagcagcct
     7981 tcggcggcaa cacgggatcg aagggatggg tttcgatttc aagcagattt tcagacacgt
     8041 tcaatcctcc aacaggcaaa acggcagaca gccggttttt gcaccaaacc gccaaacacg
     8101 gcaagccttc aaacagcatt atcacgccct gccgttttcg gttacaatgc cgacatcaaa
     8161 cggatactgt aaacacattt atgtttcaac acacaggacg acacataaag caccgcccta
     8221 tgtgccatcc tgatttggaa agagttacgc ccctcccaaa tagagtctga ttctaccgcc
     8281 ctaaagggcg gggtttcaac cgaaaaggaa acacgatgaa agaacacaaa gcccgcaagc
     8341 gtttcgggca gaattttttg caggacacgc ggattatcag cgatattgtc aacgccgtgc
     8401 gcccgcaggc ggatgatgtc gtgattgaaa tcggcccggg tttggcggcg attaccgaac
     8461 ctttggcgaa aaagctgaac cgcctgcacg ttgtcgaaat cgaccgcgac atcgtatgcc
     8521 gtctgaaaac gctgccgttt gcggataaac tggtgattca cgaaggcgat gtattgcagt
     8581 ttgatttcaa cggcattgaa ggcaaaaaga aaatcgtcgg caacctgccg tacaatattt
     8641 ccacgccgct tttgttcaag ctggcggagg tggcggacga tgtcgtcgat atgcacttta
     8701 tgctgcaaaa agaagtggtc gagcgtatgg ttgccgcgcc gaaaagcaac gactacggcc
     8761 gcttgggcgt gatgctgcaa tattttttcg atatggagct gctgattgac gtaccgcccg
     8821 aatcgttcga tcctgcaccg aaagtggatt ccgccgtcgt ccgtatgatt ccggtgaaac
     8881 accgcatcgg caaggcggac gatttcgagc atttcgccaa actcgtaaaa ctcgccttcc
     8941 accaacgccg caaaaccata cgcaacaacc tgaaagaact ggcaacagac gaagatttgc
     9001 aggcagccgg catcaatccg caagaccgtg ccgaacatat cgcgccggag aaatatgtgg
     9061 cgttgagcaa ttatctggcg ggcaaggccg tctgaaaagg gagtaagccg atgacgaaat
     9121 acctgctttt acccctgata ctgccgcttg cagcctgcct gaccaatccg aacgaccgtt
     9181 tttttgacgg cagacgctac acgcttcatc atatggagct cccgtaacga caacctgcaa
     9241 cggcaaatgc cgtctgaaag aaaagacatg atcaaattca aaaacgtaca caaacatttc
     9301 aaagacctgc acgtcatcaa cggcgtgaac ttggaaatca aaaaaggcga agtggtcgtc
     9361 gtctgcggac cttcgggcag cggcaaatcc acactcatcc gcaccgtcaa ccagcttgaa
     9421 agcatcgaaa gcggcgagat ttgggtggac ggtgtcaacg ttgccgaccc aaaaaccgat
     9481 ttgaacaaaa tccgcgaaga agtcggcttt gtgtttcaaa gtttcaacct ctacccccac
     9541 ctgaccgtat tggaaaacat caccctcgca ccgatgaagg ttaaaggtca aaatgcggaa
     9601 caggcggaga aaaaagcgat ggaacttttg gaacgcgtcg gactggcgca caaaaaagac
     9661 gcttttccgt cccaactttc cggcggccag cagcagcgcg tggcgattgc acgcggtttg
     9721 gcgatggaac cgcgcgtaat gttgtttgac gaaccgacct ccgcactcga ccccgaaatg
     9781 gtcggcgaag tgttgaaagt gatgaaagac ttggcagaaa gcggcatgac catgatgtgc
     9841 gtaacccacg aaatggggtt tgcccgcgaa gttgccgacc gcgtgatttt cgtcgataaa
     9901 gggcaaatcc tcgaagacga aacgcccgaa gcgtttttca ccaacccgaa acacgaacgc
     9961 gccaagcagt tcctgcaaca ggtcatgacc cactgatgct ataccgtctg aaaaccgaac
    10021 gttcagacgg catagcccga caccgcaaga tgattgagaa agaaaaccat gtattttgtt
    10081 gaccgcaccg ccgtcgtcct caagccgacc gtccgctttt tggaatggct caaaagcacc
    10141 gacgaaaata tgcccgacct gaccatagag cagcttcgtg ccaactgctc cgtcttcctc
    10201 gttccgcagt tcgacgaacc ggaagccgtc gtttcctatt tcgacgaacg ctaccgccag
    10261 atttttgaag ccgaactcgc cggctgggac atcgacaagg ataaatggcc gcaagatatg
    10321 gggctgaagg cgttttggga atttttcgac atcgaaatcc acgatatggt gttggatatg
    10381 gaagaagcgg atttgaacat cacgcccgtg ttcgacaata tgatgtaacg ccgtgcgcgc
    10441 ctttcagaca gcattgaggc cgtcccgcat cctgaacatc ctgaccgtat cgctccactc
    10501 ggcttccctt gccgtctgcc tgacctggtt ttacggccgg atgatgtggt tcggtttggc
    10561 ggctttggcg gcaagttacg catactcgct caggataacg aatctgaaac accgccatgc
    10621 cataaccgcc attaccatag accgcgacgg acaggcggaa atcgtatccg gcaaagaaaa
    10681 aacagcaaag gcggcggcac tgtcgggcag cagtatggtt acgccttatg ccttattctt
    10741 acaatgggac accggcggca aaaccgtccg ccactgcatc acccacgata tggcggaccc
    10801 ggaatcctac cggcggctga aagtctgggt gctttggcgg cagccgaaaa aaaccgccga
    10861 aaccgataca ccaagctgat ttcagacggc atttgaacac ggaacactca atgaaaacac
    10921 tgcaagactg gctctcccat ttggaaaccg cccacagcgg cggtttgatc gatatgggct
    10981 tggaacgcgt aggcgaagtg aaaaaacgga tgaagctcac gccgcaatgc cccgtcgtcg
    11041 ttgtcgcggg aactaacggc aaaggttcgg tctgcgccta cctgacacag atttacaaac
    11101 aggcgggcta caaaaccggc acattaacca gcccccattt attgcgttac aacgaacgca
    11161 tcgccatcaa cgccgaaccc gtttcggacg atacgattat tgcctctttc gagcgcatcg
    11221 aagcggcgcg cggcgaaata tcgctgactt attttgaatt caatacgttg gcggccgtcg
    11281 acatcttcat gcgcgaacag gttgacgtga tgatattgga agtcggcttg ggcggacgct
    11341 tggacgcggt caacgcattt gacggcgatt gcgcggtggt tactagtgta gattttgacc
    11401 atcaggcgtt tttgggcgat acggtcgagc aggtcggctt tgaaaaagca ggcgtgttcc
    11461 gcggcggcaa acccgcaatc tgcggtcaaa atcccgcgcc cgcatcgttg ctttcacacg
    11521 ccgaagccat aggcgcgaaa ctgctgatgg tgcagcgcga tttcgagttt cacgcgctgg
    11581 aaaacatcca atggaactac cgcttccgac cgcagcattc agacagcccc gctcgcaacc
    11641 gtaattccct gcccttcccc gcattgcgcg gcgcatacca actttccaac gccgcctgcg
    11701 cgctgaccgt gttggaatgt ttgaacgaca aattgccggt ggacatcggt gcaatcaaac
    11761 gcggattgct gctggttgaa aatcccggac gcttccaagt cctgcccggc cggccgctga
    11821 ccgttttgga cgtcggacac aatccccacg ctgcccgcgc cctgcgccgc agcctgatta
    11881 atttggcgtt tgcgcaaaaa cgcaccgccg tattcagtat gctgtccgac aaagacatag
    11941 acggcgtgtt ggaaatcgtt aaagaccagt ttgacgagtg gtatatcgca ccgttggacg
    12001 taccgcgcgg catgacgctc gagagcctgc aaaacaaact gcacgaacac agcatcgaaa
    12061 acatacagac attcgccgcc gtccgcgaag cataccgggc ggcagcctca aaagcgggcg
    12121 agaacgacag aatcgtcgtt ttcggctcat ttcataccgt tgccgatgtc atgtcggtac
    12181 tttaaaagga aacttctatg tccgaaaaca aacaaaacga agtcctgagc ggttacgaac
    12241 aactcaaacg gcgcaaccgc cgccgcctcg taacggcaag ttgcctggtt gccgcctcct
    12301 gcatcctgct ggcagccgcc ctcagttccg gccctgccga acagactgcc ggcgaaacaa
    12361 gcggcgtaga aaacaaagcg gcaggtgcgg cacaaacccc tgccttgaaa tccgccgccg
    12421 acaaaccgca ggacttggca ggcgaagaca agccttctgc cgccgacagc gaaatcagcg
    12481 agcctgaaaa cgtaggcgcg ccgctggtgc tgattaacga ccgcctcgaa gacagcaaca
    12541 tcaaaggttt ggaagcatcc gagaaactgc aacaggcaga aaccgccaaa accgcaccga
    12601 agcaggcaaa acaacgcgct gccgaaaaag tgccggcaac tgccgacagt acggatacgg
    12661 tagcggttga aaaaccgaaa cgcactgccg aaacaaaacc gcaaaaagcg gaacgcactg
    12721 ccaaagccaa gcccaaagcc aaagaaacca aaaccgccga aaaagttgcc gacaaaccga
    12781 aaactgccgc cgaaaaaacc aaaccggata cggcaaaatc cgacagcgcg gtaaaagaag
    12841 cgaaaaaagc cgacaaggct gaaagcaaaa aaacagccga aaaagaccgt tcggacggca
    12901 aaaaacacga aacggcacaa aaaaccgaca aagcggacaa gaccaaaacc gccgagaagg
    12961 aaaaatccgg taaaaaagcc gccattcagg caggttatgc cgaaaaagaa cgcgccttaa
    13021 gcctccagcg caaaatgaag gcggcgggta tcgattcgac catcaccgaa attatgaccg
    13081 acaacggcaa agtttaccgc gtcaaatcaa gcaactataa aaacgcaagg gatgccgaac
    13141 gcgatttgaa caaattgcgc gtacacggta tcgccggtca ggtaacgaat gaataacctc
    13201 cccgttgccg acctcctcgt cttcgccatc atcgccgcct gcatcgtgct atccgcgatg
    13261 cgcggcgtga ttgcggaggc aggttcgatg gcggcatggg tggtttcctt cttttttgcc
    13321 aaactctttg ccgcaccctt cgccgacctc gcctttgcct cgttccagcc gcgcctgttc
    13381 gcactggcat tgtcgtttat ttcattattc atcctcgcct atctgataca gaaaatactc
    13441 cgctcgctgc tgacacaagc agtttcctcc gtcggcttgg gctttgcaaa ccgcattttg
    13501 ggcggcgtat tcggtgcatt gaaaggcgtt ttgattgtta ccctgctgat catgcttgct
    13561 tcaaaaaccg acctgcccga taccgaagaa tggcggcaat cttacacact gccgtttttc
    13621 gtatcgcttt ccgaagcagt gttaaaccat gcggacgaca gtgatggagc tttggaagac
    13681 gattgattgc catacggata tttcagacgg catcctcttt tatgccgtct gaaatgtttt
    13741 ttcctttcct cagccatatc acagattata gtggattaaa tttaaaccag tacggcgttg
    13801 cctcgccttg ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
    13861 taatccacta taaatcttat tctccataaa cccacctatc tgtccttcac tcaaatattg
    13921 tcaacaattc aggcaaaaaa ccgccgtttg ctttataatt ccgttttacc cttaaacctc
    13981 gaatcggaga agacgatatg tgcggcgtat taggtttggt cagtcatgca cccgtgaacc
    14041 agcttctgta cgacggcttg cagatgttgc agcacagggg gcaggatgcg gcgggcattg
    14101 caacggcgga aggcggcacg ttccatatgc acaaaggcaa aggcatggtg cgcgaagtgt
    14161 tccgtacacg caatatgcgc gatttgaccg gcaacgccgg catcgcccac gtccgttatc
    14221 ccacggcggg caacgccggc agcagcgcgg aggcgcagcc tttctacgtc agctcgccgt
    14281 tcggcatcgt tttggcacac aacggcaacc tcaccaacac ggccgaactg tatgaaaacg
    14341 tgtgcaacaa acacctgcgc cacatcaaca ccggctccga ttccgaagtc ttgctcaacg
    14401 tattcgcgca cgaattgcgc cgcgaagttt ctaaaaacgc cgacccgcac cggctcaatg
    14461 ccgacaatat tttcaacgcc gttgccgaag tccaccgcct ggtgcgcggc gcatacggcg
    14521 tggttgccat gattgcgggc tacggcatgg tcgccttccg cgatccttat ggcatccgtc
    14581 cgctggcatt gggttcgcaa accgacgaat caggcagaaa atcctatgcc gtcgcctccg
    14641 aatccgttgc cttcaatgcg cttgcctacg atttggaacg cgatatccgg ccgggcgaag
    14701 cggtatttgt cggctttgac ggcacaatga ttgcccgcca atgcagcgat aaggccaaac
    14761 tcagcccctg cctttttgaa tatgtttatt ttgcccgccc cgactctgtg attgacggcg
    14821 tatccgttta ccaatcgcgc ttggatatgg gcgtgtcctt ggcggaaaaa atcaaacgcg
    14881 agctgcccgt ggacgacatc gacgtcgtga tgcccattcc cgacaccagc cgccccagcg
    14941 cgatggagct tgccgtctac ctcaaaaagc cttatcgcga aggcttgatt aaaaaccgct
    15001 atatcggccg cacctttatt atgcccggtc aggcgacgcg caaaaaatcc gtgcgccaaa
    15061 aactcagccc gatggaaacc gaatttgcag gcaaaagcgt gttgctggtg gacgactcca
    15121 tcgtgcgcgg gacgaccagc cgcgaaatcg tcgaaatggt acgcgcggct ggcgcgcgca
    15181 aagtctatat cgcctccgcc gcgcccgaag tgcgctatcc caacgtatac ggcatcgata
    15241 tgcccacacg cgaagagttg attgccaatg ggcgcagcgc agcggaaatt gccgccgaaa
    15301 tcggcgcgga cggcatcgtg tttcaagact tgggcgattt ggaagccgtc gtcaaagcac
    15361 tcaatccgaa aatcgaatct ttcgattcgt cctgtttcaa cggcatttat caaaccggcg
    15421 acatcgatga tgcctacctc gaccgcctgt ctgccgagaa atccggctgc ggcggcctga
    15481 aaatccaccc gagcaggatg gaacacagca tcagcatcag cgatgcgggc gacgaagaat
    15541 aaggcttccc aaaccgcgat gccgtctgaa atccgaacgc cgggttttca gacggcattt
    15601 cccatatcgg catcacattt cccatttcat ccgcacgcaa tatgagtacc gaaaccaaaa
    15661 actacattac gcccgccgga tggcaggcat tgaaagacga gctttaccgg ctggtcaaca
    15721 aagaacgccc cgaaatcgtc caaatcgtca actgggcggc aggcaacggc gaccgcagcg
    15781 aaaacggcga ctatctttac ggcaaacgcc gtatgcgcga aatcgaccgc cgcatccgtt
    15841 tcctgaccaa acgtttggag tctgccgtcg tcgtcgatcc cgaattgcgc gaagcgaccg
    15901 accaagtgtt tttcggcgcg acggtcgggc tgttgcgcgg cgacgggcgc gagcaaaccg
    15961 tcaaaattgt cggcatcgat gaaatcgata ccgcgcaaaa caaaatttct tggatttcgc
    16021 cgttggcgcg ttgcctgatt aaagcgcgcg aaggcgacga agtggttttg aacacgccgg
    16081 aagggcgtga ggaaattgaa atcctttctg tcgaatacat caaaatcgac tgatcgggaa
    16141 tgccgtctga aacggcacaa acgcatcgtt tcccaaataa aggaataccg aatgaggaaa
    16201 atccgcacca aaatctgcgg catcaccaca ctggaagacg cagcggccgc cgcagcggca
    16261 ggtgcggatg ccgtcgggct ggtctttttc caaggcagca gccgggccgt cgatattgcc
    16321 cgcgccaaaa aaatcaccgc cgcactgccg ccgtttgtca gcgttgtcgc ccttttcgtc
    16381 aacgaaagcg cgcaaaacat ccgccgcatc cttgccgaag tgccgataca catcatccaa
    16441 ttccacggcg acgaagacga cgcattctgc cgccagttcg accgccccta tatcaaggcc
    16501 attcgtgttc agacggcatc agacatccga aacgccgccg accgcttccc cgacgctcag
    16561 gcactgctgt tcgatgccta ccacccttcg gaatacggcg gcaccggaca ccgcttcgac
    16621 tggacgctgc tggcggaata ttcgggcaaa ccgtgggtgc ttgccggcgg gctgacccct
    16681 gaaaacgtcg gcgaagccgt ccgcatcacc ggagcggaag cggtcgacgt atccggcggc
    16741 gtggaagcgt ctaaaggcaa aaaagaccca gccaaagtcg ccgcctttat cgcaaccgcc
    16801 aaccgcctat cccgttaaag caacaaaaat tgccgctgga atga
//
LOCUS       DAWFIE010000042        16727 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWFIE010000042 DAWFIE010000000
VERSION     DAWFIE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16727
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.042"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(77..1246)
                     /locus_tag="WGQ71_001251"
     CDS             complement(77..1246)
                     /locus_tag="WGQ71_001251"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ0707261.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1429..1539)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1592..2488
                     /locus_tag="WGQ71_001252"
     CDS             1592..2488
                     /locus_tag="WGQ71_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ0707262.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMCQAGMNPDPKTVKAVFAETAKGGLELALAFFKKPEDIETMF
                     KSVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQRLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2532..3596)
                     /gene="tsaD"
                     /locus_tag="WGQ71_001253"
     CDS             complement(2532..3596)
                     /gene="tsaD"
                     /locus_tag="WGQ71_001253"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ0707263.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(3713..4900)
                     /gene="ccsB"
                     /locus_tag="WGQ71_001254"
     CDS             complement(3713..4900)
                     /gene="ccsB"
                     /locus_tag="WGQ71_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ0707264.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4893..6845)
                     /locus_tag="WGQ71_001255"
     CDS             complement(4893..6845)
                     /locus_tag="WGQ71_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ0707265.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILSASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7122..7745)
                     /locus_tag="WGQ71_001256"
     CDS             complement(7122..7745)
                     /locus_tag="WGQ71_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ0707266.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7951..8580
                     /locus_tag="WGQ71_001257"
     CDS             7951..8580
                     /locus_tag="WGQ71_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ0707267.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKEN"
     gene            complement(8701..11097)
                     /locus_tag="WGQ71_001258"
     CDS             complement(8701..11097)
                     /locus_tag="WGQ71_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ0707268.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11249..12364
                     /locus_tag="WGQ71_001259"
     CDS             11249..12364
                     /locus_tag="WGQ71_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ0707269.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12367..12966
                     /locus_tag="WGQ71_001260"
     CDS             12367..12966
                     /locus_tag="WGQ71_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ0707270.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12967..13614
                     /locus_tag="WGQ71_001261"
     CDS             12967..13614
                     /locus_tag="WGQ71_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ0707271.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13632..14177
                     /locus_tag="WGQ71_001262"
     CDS             13632..14177
                     /locus_tag="WGQ71_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ0707272.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14196..16481
                     /locus_tag="WGQ71_001263"
     CDS             14196..16481
                     /locus_tag="WGQ71_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ0707273.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAATPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWEAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
BASE COUNT         4522 a         4746 c         3803 g         3656 t
ORIGIN      
        1 cctgattttt gttaatccac tatataaaaa tgccgtctga actgttcaga cagcattttt
       61 gattttcaaa ccggaattac agccccgctg ccgccctcaa tgcagcagct ttgtcggtgc
      121 gctcccaagt gaactcaggt tcttcgcggc cgaaatgtcc gtaagcggcg gatttactgt
      181 aaatcgggcg caagaggtcg agcatttgga cgatgccttt ggggcgcagg tcgaaatgtt
      241 cgcgaactaa ggtaatcagt ttttcttcgc tgattttgcc tgtgccgaaa gtatcgatgg
      301 aaatcgaagt cggttcggca acgccgatgg cgtaggaaac ttggatttgg cattgggttg
      361 ccaagcctgc ggcgacgatg tttttcgcca catagcggca ggcgtaggcg gcggaacggt
      421 caaccttgga cggatctttg ccggagaacg cgccgccgcc gtgcggagcc gcgccgccgt
      481 aggtatcgac gatgatttta cggccggtca aaccgcagtc gccttgcggg ccgccgatga
      541 cgaagcggcc ggtcgggttg atcaggtatt tggtttcgtc ggtcagcagt tcagacggca
      601 ggacgggctt gataatctgc tcaatgacgg cgtttttcaa ctcttcataa ccgatttccg
      661 gatcgtgttg cgtagagagg acgacggtat caatgcgttt taccttaccg gtttcgctgt
      721 cgtaaaccac ggtcagttgg gctttggcat caggacgcag ccacggcagg cggccgtctt
      781 tgcgcaattc gctttggcgt tgcatcaggc ggtggctgta atagatggca aacggcatca
      841 gggtcggggt ttcgtcgcag gcgtagccga acatcagacc ttggtcgcct gcgccttggt
      901 tcaagtcgat gccttcgcct tcgtttacgc cttgggcaat gtccggagat tgttggtcgt
      961 agtacacgcc gaccgcgcag ccgttggcat caaagcccag ctcggaggag ttgtagccga
     1021 tgcgtttgat ggtttcgcgt gcgactttga tgtagtccac ttgggcggtg gtggtgattt
     1081 cgcccgccaa tacgcacaag cctgtgttga ccaaagtttc tgcggcgaca cgcgctttgg
     1141 ggtcttgcgc cagaatcgca tccaaaatag catcggatac ttggtcggcc actttgtccg
     1201 gatggccttc ggataccgat tcggaagtaa acagatattc gctcattgag attttccttg
     1261 aaaaacaaac catcttcgtt agacggcatg ttgtatgaac ataatgtcga cagcgggaaa
     1321 tatagcaaaa tttccctatt cgtgccattc agttgagaaa cattcccatt tgaatggcac
     1381 tttggaatct ctgcccgtac gtttcgtaca ggcaaaaaaa ttcccgcatc aagcgggttt
     1441 ggattgctct ggtgagccac atcggctttt caaccgtcca ccttactttt ccttttgaaa
     1501 agcaggttgg catggaattc ccaactctta atgcagcccg gatggtagca gaaaagcctt
     1561 tccgccgcaa tacttccctt tttcagacgg catgtttcgt ttacaattcg ggctgtttcc
     1621 ccctttgcga accgccatgc acatcctgtt gaccgccctg ctcaaatgcc tctccctgct
     1681 gccgctttcc tgtctgcaca cgctgggaaa ccggctcgga catctggcgt tttacctttt
     1741 aaaggaagac cgcgcgcgca tcgtcgccaa tatgtgtcag gcaggcatga atcccgaccc
     1801 caaaacggtc aaagccgttt ttgcggaaac ggcaaaaggc ggtttggaac ttgccctcgc
     1861 atttttcaaa aaaccggaag acatagaaac aatgttcaag tcggtacacg gctgggaaca
     1921 cgtgcagcag gctttggaca agggcgaagg gctgctgttc atcacgccgc acatcggcag
     1981 ctacgatttg ggcggacgct acatcagcca acggctgcca ttccccttaa ccgccatgta
     2041 taaaccgccg aaaatcaaag cgatagacaa aatcatgcag gcgggcaggg ttcgcggcaa
     2101 aggaaaaacc gcgcctacca gcatacaagg ggtcaaacaa atcatcaaag ccctgcgttc
     2161 gggcgaagca accatcgtcc tgcccgacca cgtcccctcc cctcaagaag gcggggaagg
     2221 cgtatgggtg gatttcttcg gcaaacctgc ctataccatg acgctggcgg caaaattggc
     2281 acacgtcaaa ggcgtgaaaa ccctgttttt ctgctgcgaa cgcctgcctg gcggacaagg
     2341 tttcgatttg cacatccgcc ccgtccaagg ggaattgaac ggcgacaaag cccatgatgc
     2401 cgccgtgttc aaccgcaatg ccgaatattg gatacgccgt tttccgacgc agtatctgtt
     2461 tatgtacaac cgctacaaaa tgccgtaacg aaaataaaaa tgccgtctga acaatttcag
     2521 acggcatttt gtcatctgac gatttccgac agcggccagc gcggacggac attgaacgca
     2581 ccgacctccc tgcccttgcc caggtgcatc gcaccggcaa aggcaatcat ggcaccgttg
     2641 tccgtgcagt atgccgtcgg cgggaaaaac acgctgactt tttcggacgg atgtttcggc
     2701 ttgccttttg gggtcgggat ttgcaccgtc atattgccga aagtttcacg gagtttgcgg
     2761 tttgcaccga ccccgccggc gaccactacg gttctgaacc ctgtctgcaa cagggctttt
     2821 ttcactttcg ccgccaacac atcgactacc gcatcttgaa acgcacggca aatatcgttg
     2881 cgggtttgct cgggaatggt ttccgaaccg tttgcctcac gcactttttc aacggcagtc
     2941 aaaacagcgg ttttcaaacc tgaaaaactc atctgcaaat cgtcggaatg aatcatcggg
     3001 cgcggaaaaa caaacgcttc gggcctgccc gattccgcaa gttccgacag tttcgcaccg
     3061 cccggatacg gcaagcccag cagtttcgcc gttttgtcga atgcctcgcc cgccgcatca
     3121 tcgacgctct cgcccaaaag cgcgtagtcg cctatgcccc tgaccgccat aatctgcgta
     3181 tgcccgcccg aaaccaacag cgcgacaaaa ggaaagtcgg gtttttcctc cgccaacagc
     3241 ggcgacagca gatgtccttc caaatgatgg acgggaataa caggcttgtc caacgctaaa
     3301 gccagcgcat tggcgtaact cgaacccgcc agcaacgcac cgcccaaacc gggcccctgc
     3361 gtaaaggcaa ccgcgtcaat gtcgccatac gatgcgcccg cctgcgccaa acagccttca
     3421 gtcaacggaa caaggcggcg gatatggtcg cggcttgcca attccggcac aaccccgccg
     3481 tattcggcgt gcattgccat ttgagtgtgc aggcagtgcg cccgcaatcc acgttccgta
     3541 tcgtaaagcg caacacctgt ttcgtcgcaa gaagactcga ttcctaatac caacatggtc
     3601 tgatgccgtt aaaaactgaa aaacgtattt tagcggattt cggcacgact gccgtatccc
     3661 aaaaacggaa catgccgtct gaagaccgtt cagacggcat cgtcgcaccg tatcaaagcg
     3721 ttccgtaaga atgcagcccg ctcaaaaaca tattcacgcc gataaaggca aatgcggtta
     3781 cgaacaaacc gataatcgcc caccacgcca gcactttgcc gcgccaaccg gcaaccagcc
     3841 gcaagtgcag ccaaacggcg taattgagcc agacgatgaa cgcccacgtc tctttcggat
     3901 cccaactcca atagcgtccc caagcatctg ccgcccacag cgcacccaaa atggtggcaa
     3961 tggtaaagaa cagaaagccg acggcaatcg ccttatacat cacctcgtcg atcaatgccg
     4021 acggcggcag ccacagtttt ccgccttttc cttccgcacg cagggaaacc agttcggcaa
     4081 taccgagcat cgcggaaatg caaaacgcgc cgtaaccgat aaagtttgcc ggaacgtgga
     4141 ttttcatcca ccaggactgg agcgcgggaa tcagcggctg gatggtatgc gcctcgcggg
     4201 acacgctgta ccacaagaca aatccaacca cgaccgccat aaagccgaac acgaagccgc
     4261 ccaatttctg tatggcgaac ttaccttcat aataaagata catcagcgcg gtaatcacca
     4321 aaaacaggat gaacacttca tacaggttgg aaaccggaat atgccccgca tcgggacgga
     4381 gcagatagct ttcgtgccaa cgtaccagca gaccggtaaa gcctgctacg gcagacaccc
     4441 atgcaaacac ggttcccata cccaacagcg tgttggtcgg cacatttttt acgcttgcca
     4501 aaaccgcgcc cgaaatatag gcgaacaggg cgaaaaagac aaaggcgcac tgccacatga
     4561 tcgccgactg gctgctgagg aaataccgca acaggaaaat ctctgccgat ttgatgttgc
     4621 cgccatacag accgacggcg gcataggcaa gcaatacgct taaaggaaca aaccagcgca
     4681 tcggtttgaa aaaccaaccc aaaaacacgg caataccggc actcgcccac aacatgaccg
     4741 tttcgtaaat gtccatatgc ataccggaac gggtctgtac gaaaaccgta gccgcaaaaa
     4801 ccagcacggc aaatacccaa tcccaaagat tcagattgct gatcaaagac ttctgaatca
     4861 gcagctcgtg ttccggaagg gttttatagt gttcagtcat gattcaagtc cttgccgagc
     4921 cgttgcagac tctcgacgtg ttttggaaat tccttctgca aatcccgttc gctgcgggcc
     4981 gaagacatgg caaaacggat tttgccgtct gaaaacaata cccacgcccg tttttcgcgc
     5041 acataaaaca tcaataccgt acccaatacc aacagcaccg agccgagata gaccaaaagc
     5101 gcacccgggg aacgggtcat ctgcaaaccc gacgaacgca cctcggaaaa cccatcaagt
     5161 tgcagcagca taggcgcggg atattcggtc aaacccgtgt acgcatccat actgtgcagc
     5221 aggaaacggt tccgcgcttc atcctgctgc cattcgggca agccgtaccg gcgtatggtt
     5281 tcatccaaag cagcgttcat cacgccgtaa agcatttcgt agaaataacc ctgcatctta
     5341 tcctgctgct ctttcgggat attggacgta ataaattcgt ccaatcccaa atagcctttt
     5401 tgtgcaaaga tgttcagcgt gttttccgca gccagcatga attgttcgcg gatttcggca
     5461 ggtgcgtctt tggttgcgtc ggcaaccaga cgtttgcgcc cttccccatc tttcaaaaac
     5521 tcacgcaatg ccataaaggt gtccgctttc aactgcttgt ccaaggggat acgcagccag
     5581 cggtattgct gctgcaagcc gctgcgcgtg ccggtaatcc aaaaataatc ctgttcctgc
     5641 aaaaccggca gcatatagtt tttatattcg accgcctgcc ctgccgcatc acggatacgg
     5701 taaacaatgg aagggccgat attggtgtat tttttacctt cctgagtaac ggcgcggaca
     5761 tcgttcagcg tggatttcag gcttttttcc cgttccgcgc cctcgctcat gtcctccaca
     5821 ttcatagaag tgaactgatc gaactcaaga cgatatttgt gtttgccaat ttccaacgga
     5881 aactggtgta tggatgttgc cttcaacacg acaggctcgc gcgaagcatc acccaaattc
     5941 cacgccttga atgtcaaatc cgaaccgccg tcggcaaaac tcgcctgata aatcgtgatg
     6001 ccgtgcaagg tcaaaggatg gttcacgcgg atggtgcgct cgagtttctc accggttgcc
     6061 ttgtccgtca cttcaatatc gctggcgaaa tcacgcggca tacccgtatt gtaaaaatcg
     6121 atatggaatt ttttcagttt gacttcaaaa ggcaagtcct gaaccaatat cccgttgtcg
     6181 gcattcagga aaaccacatc cgcactctgc ccctcggaaa tattgacgtt gcccctaaat
     6241 gagagattgg acgcactcaa aatactttcg ggcttgaaat ccttggcata aaccgcctga
     6301 ttgtccggaa caatccgacc ggtcagcata cccagtttca acagcaggtt actgtctatc
     6361 aacccgccca ggcaaatgac aatcaaagca acatgggcaa agatatagcc ccatttgttc
     6421 attgtgcctt ttttggcggc aatcagaacc gacccgtctt cacggttaat ggtttttccc
     6481 tgaaaacctt gtacttccag ataacgtttg gcaacctcgg gcgcaatttt tacatccaac
     6541 agcgaagaat ggcgcatcgc cgccagagat ttttctttaa ccttttcccg aaaagacttc
     6601 atttcgcgcc agaacggcgg cacattgcga atcaggcaca aactggtaga aaccaccaaa
     6661 aacatcatga taacgacaaa ccatgccgaa gcatagacgt catacagtcc cagaaaacca
     6721 aaaatctgcg cccaaaaagg tccgaatttg accaaataat ccgtctgcgg ctggttttgc
     6781 tgcaacaccg taccgataac cgatgcaata cccagcagac tgagcaaagc gaccgcaaag
     6841 cgcatggagc tgaaaaaagc gaaccacgga cgggaaagaa gtgggggaga tctacgggat
     6901 ttactcattg tgtgtttatt ccgccatcag gaatatggga aagcagaatt gggcaaacag
     6961 aaaacaacgt cccgattcta ctgtcttgat gctttttatt tcaagacaat gaagacagcc
     7021 tgcatcgatt ccaacggttg cgattgaaaa aacttatcgc agaattgcct gaagccgtct
     7081 gaaaactttt cagacggcct ctaaaacaga ctattgcgga attaacgcaa accttggata
     7141 aagttggcga ccgctttcaa atcttcttca gacatacggt ttgcaatatc ttccatgatg
     7201 gtatttttac gctgaccgga cttgtaggca ttcatctgtt caacaatata tgcctgatgc
     7261 tgaccgccca aacgcggata agcctgaatt tcgcttccgc ctcccggcat acccgcaccg
     7321 ctcggaccgt ggcaggacat acacgccggc acttttttat cgctcaaacc gccgcgatag
     7381 attttcgcac ccaattcggg attttcctta ggattggctt caccggattt gggctgctgt
     7441 ttggcataga atgcggatac gttcaaaata tcctgatcgc tcaaattcat taccaccggt
     7501 ttcatcacag ctgccgaacc gtgggtgcgt ttaccgtcgc ggatgccgat agtttgatga
     7561 tagatgtaag cagtatgctg tgccgccaaa cgcggataca tcgcaatgcc gctgttaccg
     7621 tctgctgcat ggcaagccgc acaaaccgtt gcggcaacct gtttgccttt ttccacgtct
     7681 gctttgggag aggcggaaac cgcaccggca gccaaaacaa aggccaataa agtcaatcgt
     7741 ttcatggagt gctcctgatt acagcattgg ataacgcaac aatgctcttt ttatattcaa
     7801 atacgggatt tttgacccga ttaaaaccga tgattctgca aacgtgttat tctatactaa
     7861 atttacatta aattaccact gtgtttcaca taaaaccaac cgcatatttt tgctgtcgga
     7921 caaacggcgg cggaaaacaa ggatatgccc atgaaccttt ttcaaaacgc caaattcttc
     7981 acgacggtaa accatctcaa agacctgccg gacacaccgc tcgaaattgc ctttgtcggc
     8041 aggagcaatg ccggaaaatc cagtgccatc aataccctga ccaaccatgt ccgtcttgcc
     8101 tacgtttcaa aaacacccgg acggacgcag catatcaact tcttcgagct gcagaacggc
     8161 aattttatgg tcgatttgcc cggctacggt tatgcccaag tccccgaagc agtacgcgca
     8221 cattgggtca atctgctcgg cgactatctg caacagcgca aacagcttat cgggctggtt
     8281 ttgattatgg atgcccgcca tcctttaaaa gaactcgaca tccgtatgct ggactttttc
     8341 cacacgaccg gcagaccggt tcacatcctg ctgtcaaaag ccgacaaatt atccaaaaac
     8401 gaacagataa aaaccctgtc ccaagtcaaa aaactgctca aaccttattc cgacaggcaa
     8461 aacatcagcg tacagctgtt ttccagcctg aaaaaacaag gtattgacga ggccaaccga
     8521 actgtcggaa gctggtttga cgcagcagat gccgccgctt cctctccaaa ggaaaactga
     8581 ccccaattat acggaaaccg tattcccccc acttgaccga ccgcaaacat ttaaaaaatt
     8641 gccactgcca aatctaaaat gccgtctgaa agacttttca gacggcattt tgcggagtct
     8701 ttaaaacaga gaatccaact gctgctgttt ggaaccagta ttactcggaa gcaccggcgt
     8761 ttcctgcata tcttggcgga cttcgtcatc cgccgcctgc cgtccgcctt ctgccgcgcc
     8821 cccgtcatct tcttttgccc gtcgggaagg ttgcggcgca ataccgctgt tgtccagcgt
     8881 caagcccgga tcggttacca tacgttcctt catatagtat tcgccattgc tgctgaccac
     8941 accttcaggc attttcatcc ccttgccctg ctttcctttc aacgcaaaac gcatatagtc
     9001 cacccaaacc ggcaccgcaa tcgtaccgcc gtagccgaca cgccccatac tcttaggttt
     9061 gtcgaagccg atatatacgg cagtaaccac atcagggtta aaaccgacaa accacgcatc
     9121 cttattgtca ttggtcgtac ccgttttacc ggcaatatcc gttcttccca acgcagctgc
     9181 cccccttgcc gtaccaacac ggaccacatc ctgcataatc ttatacataa tataggcatt
     9241 gcgcggatcg attgcctgag gcgcattttg cccggccacc aaaggttgca tttgggcgcg
     9301 caacctgccg tctctgtcat aaatcttatc gattacgtgc gaagaaaccc tatatccgcc
     9361 gttcgcaaat acgctatatg cctccgccac tttcaacggc gttgtctcgc ccgtacctaa
     9421 agccatagac aggcttgccg gcagctcgga cgacctgaag ccgaaacgcc ggatatactg
     9481 ttgcgcgtaa ccgacaccga tagacatcaa aatacggatg gaaaccatat tcttggaagc
     9541 cgtcagagcc tgtctcaaag taatgtagcc ggaatatctg ccgtctgaat ttttaggtgt
     9601 ccaaaccgaa ccgttcggcc ctttccccgg cagggaaatc ggcgcatcgt taaccactgt
     9661 ggacgcggtc atccccttag ataatgccgc cgaatagaca aacggcttaa aggtcgaacc
     9721 cggctgccgc attgcctgaa cggcacgatt gaatgttttg ctgtgaaaat cataaccgcc
     9781 gaccagcgcg cgcacagctc cggtttttgc atccagcgaa accaaagccc cctgcagcaa
     9841 cggctcttga accaccgccc aacgcccgcc gttgtttttg acacggatga ccgcgcccct
     9901 gcggatacgg tcctccccca ttttttcatt attgaccgcg cgggccgcaa aacccaaggc
     9961 gcgcctgtca agcgtaaccc gcctgccgcc gggcagctgt atgacgacat ttttcttttt
    10021 agtcacatcc aacacaacgg cgggaaccat tttatcgacg gtatagagtc ccgacagata
    10081 ctggctgaca gtctcctcga catcttcact cttactcaaa tcgatatagt tttccgcacc
    10141 gcggtagctg ctgccgcgat cgaaattccg tagagccttg cgcaatgcct cggttgccac
    10201 cttctgatga tcggcgcgga ccgtggtata aaccttaaaa ccctgcgtat aggcatcttc
    10261 accgtatttc tcatacagtt cctgacgcac catttccgcc acatataaag cactctgatc
    10321 gattttccga acaaaccgct cgtaatgcag ttcctcattc aacgcctgat cgcgctgttg
    10381 cacggtaatc atcttctcct cgagcatatt gttcaaaata tacttctggc gcaacttggc
    10441 acgttctgga ttaacaatcg gattataggc agacggagcc ttgggcagtc ccgcaagcat
    10501 ggcggcttcc gccaaagtca aatctcggac attcttattg aaatagattt gcgcggcaga
    10561 tgcaaaacca taggcgcgct gaccgaggta aatctgattg aaatacaact cgaggatttt
    10621 gtctttgctt aaagactgct cgattttata ggcaagcaac acctcattga atttgcgtgt
    10681 gaacgttttt tcactgctca aataaaaatt tttcgccacc tgctgcgtaa tcgtactcgc
    10741 acccgactgc acgctgccgg acacgacatt gccgacggca gcgcgggcaa caccccaaac
    10801 atccaccccc caatgccggt aaaagcgttt gtcctcggcg gcgataaccg cattccgcaa
    10861 cacctctggg aaatcgccga tttttgtaaa ttcgcgccgc tgctccccat acataccgat
    10921 gacttcccca tccgccgaat aaatagtcaa cggcatttta ggctggtaat gctgcaaaga
    10981 atccaaagac ggcagtttcg gatacgttac caaaatagca atggcaacca aacccactcc
    11041 aaatacacaa aacccaaaaa ccaaaccaaa acaagtcgtt aaaatctttt taatcatagc
    11101 tgaataataa tttaccatta ttggtattaa ataaagtaaa atagcaaccg atttctacaa
    11161 agcacggttt caatgtgcaa agaacaagga atccattacg gataccgaaa cggttactca
    11221 ctgtacaaat aaagcaggaa ctttcatcat gcgcttgttt aaaagcttga aaaaccctaa
    11281 aaaaacagat gccaagctcc ctaaaaaatc ttcgggactc aataaccgcg cggcaatcgg
    11341 catcgatatc gaccagcatt ccatcaaaat ggtccaattg tcaggacgta gtttaaacca
    11401 aattcaattg gaaaaatacg tcattgccaa attaccaaag aatatcattc aaggcaataa
    11461 agtccaaaat tacgatcaac ttgttacata tttgcaacaa gcctatgcca aactgggtac
    11521 ttcgtgcaaa aacatcatcg cgtccgtccc gcaaaatctg gcaaccatcg aacaattgac
    11581 ctacacagac aaagatgcag aattagacct gcaggggttc gtggagtcct ccatctccga
    11641 agtcagctcg atatcgctcg aagaagccaa ttacgactat caggtcttgt cccaatcggc
    11701 cgccggcgaa gctgtgttgg ccgtcgcatc gagaaaggat gaaatcgaac ccctgattga
    11761 cgcattcaac gccgccggta tgaaattatc cgcgcttgat gtggacattt tcggacaata
    11821 caacgcctac gcgctatgga taaaccattt cgcccccgag cttgcagccg aaaaagtcgc
    11881 cattttcggc gtatatgccg cacagaccta cgccttggtc atccaagacg gaaaaatcct
    11941 atacaaacag gaaacctccg tcagcgaaga acagctcaac caactcatcc agcgcaccta
    12001 tcaggtaaca gaagaaaaag cggaagaaat catcaactcc ccgcaaaaac cttccgatta
    12061 ccaagaaagc gtggcaaact atttcaacca gcagattacc caagaaatac aaagggtctt
    12121 gcagttttat tacaccacgc agaccgcaga cgatatgacc gacatcaagc atatcctgct
    12181 gaccggggaa gcggcgcgcc aggaaggcat cgcccaaacc gtcgcctcac aaaccaatgc
    12241 agatgtacaa tgcgtccatc ccgcgcgtta ttttgcggac aacctcaaaa cagacaaaca
    12301 acaattcgaa cttgatgcgc cgacactgac cagggcgttc ggtttggcgg tacggggatt
    12361 ataattatga acaatttaat caaaatcaac ctcctcccct acagggaaga gatgaacaag
    12421 cgcaaacagc agcagtttaa aacgctgatg tacggtgccg tgctgacggg cgttgccgcc
    12481 gttgcggcaa cctacctgtt tatcgacaat atgatcaata accagtcgga aagaaacacg
    12541 ctgctggaaa cctccatcgc acacttggat accgagctgt cggaaataca aaagctcaaa
    12601 caggaaaaag atgccttcct gattaagaaa aacaaaatcg aggagctcca gctcaaacgc
    12661 ctccaagccg caaaaatcct cgacagcctg aatgaggccg tccccggaag cacctacctg
    12721 acctcgctgg atgccgttac cgccgactct tatcggctca gcggcaggac atccagcgac
    12781 aaccgcgttg ccgccatgat gagggcgatg cccaataccg gcatattcaa gcaacccgaa
    12841 ttgttaagca tcaagaaaaa caattcgcat caagaattta cccttcaggc aacattacaa
    12901 cccatcgtaa aggcggccga atccaaagag aatccggctt cgggaaacgc acaggaggca
    12961 aactgaatgg cttctaaatc atctaaaacc aacttggatc tcaacaacct tcacctgctc
    13021 aaccttcctg ctaggctttt tatcgccctg ctggccgttg ccgccgtgct ggggctcggt
    13081 tatgccggat tgttcaaaag ccagatggaa tcccttgagg aatacgaagc aaaagaaacc
    13141 gaactgaaaa acacctacaa acagaaaagt atcgacgcgg ccagcctgaa caacctgagg
    13201 gacgaacttg cctcaatccg ctctgccttc gatatcatgt tgaaacagct gccgacagat
    13261 gcagaaattc ccaacctggt tcaagagctt catcaggcag gttcgagcaa cggcctgcgc
    13321 ttggacagcg ttatgcccca acctcccgta gatgacggcc ccatcaaaag attaccctat
    13381 tccatttcca ttaccggaaa ttacgaacag atcagccaat ttacccgcga tgtcggcagc
    13441 ctctcccgaa tcattaccct tgagtcgctg aaaatcgccc aatctccgga aaacggcggc
    13501 aatcctgacg gcaagagcag catcctgaac ctcagtgcca ttgccaccac ctaccaagca
    13561 aaatccgtag aagagcttgc cgcagaagcg gcacaaaatg ccgagcaaaa ataacttacg
    13621 ttagggaaac catgaaacac tatgccttac tcatcagctt tctagctctc tccgcgtgtt
    13681 cccaaggttc tgaggaccta aacgaatgga tggcacaaac gcgacgcgaa gccaaagcag
    13741 aaatcatacc tttccaagca cctaccctgc cggttgcgcc ggtatacagc ccgccacagc
    13801 ttacagggcc gaacgcattc gacttccgcc gcatggaaac cgacaaaaaa ggggaaaatg
    13861 cccccgacac caagcgtatt aaagaaacgc tggaaaaatt cagtttggaa aatatgcgtt
    13921 atgtcggcat tttgaagtct ggacagaaag tctccggctt catcgaggct gaaggttatg
    13981 tctacactgt cggtgtcggc aactatttgg gacaaaacta cggtagaatc gaaagcatta
    14041 ccgacgacag catcgtcctg aacgagctga tagaagacag cacgggcaac tgggtttccc
    14101 gtaaagcaga actgctgttg aattcttccg acaaaaacac cgaacaagcg gcagcacctg
    14161 ccgcagaaca aaattaagaa gaggattact ccattatgaa taccaaactg acaaaaatca
    14221 tttccggtct ctttgtcgca accgccgcct ttcagacagc atcggcagga aacattacag
    14281 acatcaaagt ttcctccctg cccaacaaac agaaaatcgt caaagtcagc tttgacaaag
    14341 agattgtcaa cccgaccggc ttcgtaacct cctcaccggc ccgcatcgcc ttggactttg
    14401 aacaaaccgg catttccatg gatcaacagg tactcgaata tgccgatccc ctgttgagca
    14461 aaatcagtgc cgcacaaaac agcagccgtg cgcgtttggt tctgaatctg aacaaaccgg
    14521 gccaatacaa taccgaagta cgcgggaaca aagtttggat attcattaac gaatcggacg
    14581 ataccgtgtc cgcccccgca cgccccgccg taaaagccgc gcctgccgca ccggcaaaac
    14641 aacaggctgc cgcaccgtct accaagtccg cagtatccgt atccaaaccc tttaccccgg
    14701 caaaacaaca ggctgccgca ccgtttaccg agtccgtagt atccgtatcc gcaccgttca
    14761 gcccggcaaa acaacaggcg gcggcatcag caaaacaaca gacggcagca ccagcaaaac
    14821 aacaggcggc aacaccagca aaacaaacca atatcgattt ccgcaaagac ggcaaaaatg
    14881 ccggcattat cgaattggct gcattgggct ttgccgggca gcccgacatc agccaacagc
    14941 acgaccacat catcgttacg ctgaaaaacc ataccctgcc gaccacgctc caacgcagtt
    15001 tggatgtggc agactttaaa acaccggttc aaaaggttac gctgaaacgc ctcaataacg
    15061 acacccagct gattatcaca acagccggca actgggaact cgtcaacaaa tccgccgcgc
    15121 ccggatactt taccttccaa gtcctgccga aaaaacaaaa cctcgagtca ggcggcgtga
    15181 acaatgcgcc caaaaccttc acaggccgga aaatctccct tgacttccaa gatgtcgaaa
    15241 tccgcaccat cctgcagatt ttggcaaaag aatccgggat gaacattgtt gccagcgact
    15301 ccgtcaacgg caaaatgacc ctctccctca aagacgtacc ttgggatcag gctttggatt
    15361 tggttatgca ggcacgcaac ctcgatatgc gccagcaagg gaacatcgtc aacatcgcgc
    15421 cccgcgacga gctgcttgcc aaagacaaag ccttcttaca ggcggaaaaa gacattgccg
    15481 atctgggcgc gctgtattca caaaacttcc aattgaaata caaaaatgtg gaagaattcc
    15541 gcagcatcct gcgtttggac aatgccgaca caaccggaaa ccgcaatacg cttgtcagcg
    15601 gcaggggcag cgtgctgatc gatcccgcca ccaataccct gattgttacc gatacccgca
    15661 gcgtcatcga aaaattccgc aaactgattg acgaattgga cgtacccgcg caacaagtga
    15721 tgattgaggc gcgtatcgtc gaagcggcag acggcttctc gcgcgatttg ggcgttaaat
    15781 tcggcgcgac aggcaagaaa aagctgaaaa atgatacaag cgcattcggc tggggggtaa
    15841 actccggctt cggcggcgac gataaatggg aggccgaaac caaaatcaac ctgccgatta
    15901 ccgctgccgc aaacagcatt tcgctggtgc gcgcgatttc ctccggtgcc ttgaatttgg
    15961 aattgtccgc atccgaatcg ctttcaaaaa ccaaaacgct tgccaatccg cgcgtactga
    16021 cccaaaaccg caaagaggcc aaaatcgaat ctggttacga aattcctttc accgtaacct
    16081 caatcgcgaa cggcggcagc agcacgaaca cggaactcaa aaaagccgtc ttggggctga
    16141 ccgttacgcc gaacatcacg cccgacggcc aaatcattat gaccgtcaaa atcaacaagg
    16201 actcgcctgc gcaatgtgcc tccggtaatc agacgattct gtgtatttcg accaaaaacc
    16261 tgaatacgca ggctatggtt gaaaacggcg gcacattgat tgtcggcggt atttatgaag
    16321 aagacaacgg taatacgctg accaaagtcc ccctgttggg cgacatcccc gttatcggca
    16381 acctctttaa aacacgcggg aaaaaaaccg accgccgcga actgctgatt ttcattaccc
    16441 cgaggattat gggtacggcc ggcaacagcc tgcgctattg atgcgtcaaa ataagggcat
    16501 atgttttacg gcatatgccc tttctttatg ctttttgccg cgaccgaaat gccgtcattc
    16561 ccgcgcaggc gggaatccaa cttgtccggt tccggttgtt tttcgtctcg taacttttga
    16621 gccgtcattc ccgcgaaagc ggaaatccag tccgttcggt ttcggtcgtt tccgataaat
    16681 tcctgctgct tttcatttct agattcccac tttcgtggga atgacgg
//
LOCUS       DAWFIE010000043        16578 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWFIE010000043 DAWFIE010000000
VERSION     DAWFIE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16578
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.043"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(768..1847)
                     /gene="apbC"
                     /locus_tag="WGQ71_001264"
     CDS             complement(768..1847)
                     /gene="apbC"
                     /locus_tag="WGQ71_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ0707274.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPLTGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICTNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIAKIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
     gene            2058..2133
                     /locus_tag="WGQ71_001265"
     tRNA            2058..2133
                     /locus_tag="WGQ71_001265"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2091..2093,aa:Lys,seq:ttt)
     gene            2212..2721
                     /locus_tag="WGQ71_001266"
     CDS             2212..2721
                     /locus_tag="WGQ71_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ0707275.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSSA
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            3064..3504
                     /gene="nadR"
                     /locus_tag="WGQ71_001267"
     CDS             3064..3504
                     /gene="nadR"
                     /locus_tag="WGQ71_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ0707276.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            3619..4119
                     /gene="hpaC"
                     /locus_tag="WGQ71_001268"
     CDS             3619..4119
                     /gene="hpaC"
                     /locus_tag="WGQ71_001268"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ0707277.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVTP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            4135..4830
                     /locus_tag="WGQ71_001269"
     CDS             4135..4830
                     /locus_tag="WGQ71_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ0707278.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGDTPF
                     LVVNGDVLTDIDFTAAFQTASSLPEHISAHLWLVENPPHHPDGDFSLLPDGSVQSEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(5076..5507)
                     /locus_tag="WGQ71_001270"
     CDS             complement(5076..5507)
                     /locus_tag="WGQ71_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0707279.1"
                     /translation="MGSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGRNWTFLILSGLATGASWLAYFKALQLGNASQVAPID
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            5682..7358
                     /locus_tag="WGQ71_001271"
     CDS             5682..7358
                     /locus_tag="WGQ71_001271"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ0707280.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELDIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDIGDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            7472..8563
                     /gene="ychF"
                     /locus_tag="WGQ71_001272"
     CDS             7472..8563
                     /gene="ychF"
                     /locus_tag="WGQ71_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ0707281.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMVELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVSEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVIAYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            8601..8811
                     /locus_tag="WGQ71_001273"
                     /pseudo
     CDS             8601..8811
                     /locus_tag="WGQ71_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8825..10696
                     /locus_tag="WGQ71_001274"
     CDS             8825..10696
                     /locus_tag="WGQ71_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0707282.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKNWLPGGFLGVDIFFV
                     ISGFLMTMILHREMSGGGGRFSLKAFYIRRIKRILPAFFAVLAATLTGGFFLFTKDDF
                     FLLWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLV
                     ARKSPRVQFGFLAALCALSLAASFMPSAFDKYYLPHLRACEMLVGSLTAVWMRCRQPA
                     VGRRCAAAGALFAVCILSTCLFSYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKK
                     FFQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISYHC
                     IEKPFKKWKGSFAQSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNN
                     TGKQCLWGDTEKQPELLILGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYAS
                     RVFQNWAACRAVYRYAEEHLPRYPKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLH
                     KLSSEKQAVYLMADNLASSYNVQRAYILSSRIPGYRQTLRPDDESTLKANARIRELAA
                     KYPNVYIIDAAAYIPADFQIGGLPVYSDTDHINPYGGTELAKRFSEKQRFLNTRHNH"
     gene            10758..12053
                     /gene="tyrS"
                     /locus_tag="WGQ71_001275"
     CDS             10758..12053
                     /gene="tyrS"
                     /locus_tag="WGQ71_001275"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ0707283.1"
                     /translation="MSVIQDLQSRDLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRYGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            12352..13272
                     /gene="ribF"
                     /locus_tag="WGQ71_001276"
     CDS             12352..13272
                     /gene="ribF"
                     /locus_tag="WGQ71_001276"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ0707284.1"
                     /translation="MKIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGTFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIEADMEAAKCW"
     gene            13413..16202
                     /gene="ileS"
                     /locus_tag="WGQ71_001277"
     CDS             13413..16202
                     /gene="ileS"
                     /locus_tag="WGQ71_001277"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ0707285.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSVGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGDHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGRYIGETPRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCENYDKLSDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGDYYPRYAFHFAVKEMVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFAMLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         3804 a         4492 c         4515 g         3767 t
ORIGIN      
        1 aggatgcgga atctagagaa accgtttatc cgataagttt ccgttccgac aggtctggat
       61 tcccgcctgc gcgggaatga cggacgggaa cggcgcacgg gcattgccgg aatcttttgt
      121 gtttccatta tcatactctt tttaatgcgc tattccaaga atatgatgcc ctccggcggc
      181 atcccaccct acggcttgct ataaaaatac ctaaaccgtc attcccgcaa acctacattc
      241 cgtcattccc acgaaagtgg gaatctagaa cgtaaaatct caagaaaccg ttttatccga
      301 taagtttctg cgccgacaga cctggattcc cgcctgcgcg ggaatgacgg cgcataagtt
      361 tctgtgttga tttgagtcta tgggaacggt gtaggattgg tggaatcgtt ggaatcggtg
      421 ggctgaagcc caccctataa ctcacctttt acgaatcctc ctacacccta tacaacacct
      481 tgaaaatcca ccttgtcggg aatgcctaaa tcgtcagaaa tacccgaacc gtcattcccg
      541 cgcaggcggg aatccagacc tgtcggtgca gaaacttatc gggtataacg gtttcttgag
      601 attttacgtt ctagattccc actttcgtgg gaatgacggc atcggtctgc ctttacaaca
      661 cggtttctta gattccacgt tctagattcc cgcctgcgcg ggaatgacgg catatcggca
      721 ggcgcggggc ttgggacggc attgctgttg cggtttcgga cgcggcttta ttcgacgaca
      781 attttaggga accggctgct gaagtctttg cctttgtcgg caatgctcag ggcgatttgg
      841 aatgcggcat cggtgtagat tttggcgatg gcggggtgtt cgtcgaacag ttgcgccggt
      901 gtgccgccgt ccatggcttc gcgcacgggc aggcttaggg gaagctgtcc gagcaggggg
      961 acgttgaggc gggccgccaa atctttgccg ccgtccgtgc cgaacagtgc ttcgctgtgt
     1021 ccgcagttgg tgcagatgtg gacggacata ttttccaata cgcccaaaat gggaatgttg
     1081 actttgcgga acatatccac ggctttgcgc gcgtctatca gggcgatgtc ctgcggcgtg
     1141 gttacgatga ccgatccggt tacggggatg cgttgggaca gcgtgagttg gatgtcgccc
     1201 gtgccggggg gcaggtcgat aaacaggtag tccacttcgt cccactcgct ttgaaacatc
     1261 agttgctgca atgcctgact gaccatcggc ccgcgccaga cgacggcttg gccggtatcg
     1321 acgagaaagc cgatagacat gacctgtatg ccgtctgaag attcgacggg aatgagtttt
     1381 tggtttttct gatcgggttt gcggtcgtcc acacccaaca tggtcggttg gctcgggccg
     1441 taaaggtcgg catcgagcac gccgacgcgc gcgcccatcc gcgccattgc ggcggcaagg
     1501 ttggcggtgg ttgttgattt gcccacgccg cctttgccgg aggcgacggc aatgatgttt
     1561 ttcacacctt tgatggtggt aacgccaggt tggactttat gtgtgccgat ttcagtgtcc
     1621 atggacagat ggatgtgtgt gtcgcctgtc agcggcatca ggatttcctg tatgcggtcg
     1681 gctgtttctg aggcaatgtg cgcgacgggg aagccgaaat gcagggcgat atggatgccg
     1741 tctgaacgct gttcgaccga acggacggcc ttttcgccgc caagcgtgcg tgccgtattc
     1801 ggaacggcga cggtgtcgag gagggtgcgg atgttttgta tgttcataat gtgtgtttct
     1861 gtgtgcagtt tgggaaacgg gcggaaagtg tacattgatt tcgggcggtt tgcttttcct
     1921 tgtgcgaact tttacacggt atgcggcgga ttgtgaaaat ccgttgtcaa aatatcccgt
     1981 gcaaacagtg gtctgcaatt aagcctgtaa aatttgcttg acgaggtagg atggtgctgt
     2041 ataatgcacg gcttatcggg tcgttagctc agccggtaga gcagcggact tttaatccgt
     2101 tggtcgagcg ttcgaatcgc tcacgaccca ccagataacc ggaagccaag ttttgagact
     2161 tggctttttg tttgcctgcc ggttgcgtgg gcgtaccgtt tgagagagcg tatgaaaaag
     2221 aaactgcttt cgggcatcaa atttgccgtt cagacggcat tggtgttttt attggtgtcg
     2281 ctgtttttgg attggatacg caagcccgac gaacctgccg gtgcggcagg gcggcctttg
     2341 accctgctgt cggggcagcg gctgactttg ggacagttta gccgggataa ggcggttttg
     2401 gtgtattttt gggggagctg gtgcggcgtg tgccgttatc agtcgccgat aatcgatgat
     2461 ttggcggcgg acggcgtgcc ggtcgtcggc gtggcggtac gttccggcag ttcggcggaa
     2521 gtggcggcgt atatggcaaa acgcggcttg ggctttccga ctgtcaacga tgaggacggg
     2581 ggtttggcaa ggtcttggcg gattgtcgca acgcctgccg tcgttttggt caaaaatggg
     2641 aaaatggttc actatacgac gggaatcagc agttattggg gcttgcgcgc acgaattttt
     2701 caggcggatg ttttcggtta acttttgttt ttgttaaact ttacgctatg tagagaatca
     2761 aacggcatat accttgctcg tctgatgcgg cggcttttgt tttttctgga agaaagttct
     2821 ttcgacagta aatgtaatac tttgttgaga ttgtagggaa aatagactaa gatataacta
     2881 ttaaaatatt tttagatagg attatcggaa ttaaagtctt ttatacccag tcgtccgatg
     2941 cggtttatag cgtattgttg ctatatgttc gttttgttat ataacggttg catcaaaatt
     3001 tacgcccaca ggctttcccg acggtttgaa agtttgattt tcgataactt ggagacttga
     3061 acaatgccta cccaatcaaa acatgcgtct atcaatatca gtctgataca ggcaagggaa
     3121 gccctgatga cccaattcag gcctattctg aatcaggcga atattaccga tcagcaatgg
     3181 cggattatcc gtcttttggc ggaaaacggc acgctggact ttcaagattt ggcgaatcag
     3241 gcgtgcattt tgcgccccag cctgaccggt atcctgaccc gccttgaaaa agcgggtttg
     3301 gtcgtccgcc tgaaaccttc caacgaccaa cgacgtgttt ttctgaagct gactgccgag
     3361 ggcgagaagc tgtatgagga aatcggcgaa gaagtggacg agcgttacga cgctatcgag
     3421 gaagtgctgg gccgcgagaa aatgctgctg cttaaagacc tgttggcaga acttgccaaa
     3481 atcgaggatg cgttgaactc gtaatacgcc gtaacgcgcg gaaacgtccg accgacggct
     3541 ttttgaatca aaactgctgc acatggggga tgccttgtgt gcagcattct tatatagggg
     3601 acagtttaaa ggggaaaaat ggcggatttg cagaaaactt ttcaaacttc gttccgtgat
     3661 gcgatggcat cttgtgcggc aggcgttcat gtcatcacga cagacggtgc ggcagggcgt
     3721 tacggcatta caatgacggc ggttacaccg gttacggacg agccgccgac cgtgatgctg
     3781 tgcatcaacc ggagtgcgcg aatcattccg atcctgtcgg aaaacggcag cctctgcatc
     3841 aatacgctgg cggacgaaca tcaggatgtt gccgaacatt ttgccgggct gaccgggctg
     3901 tcgcccgaag agcggtttgc ctaccacatc tggcatcgcg gcaaaacggg acaacttgaa
     3961 atagagggcg cgttggcgca cctgcacggg catattgtcg gcaaacatga aatcggcacg
     4021 cattttgtgt tttacgtcag gctcgacgaa atcaaaaact gcgggtgcaa acgccccgcg
     4081 ctgctgtatt tcagacggca gtttagattt ttagactgat attcggacag atatatgaaa
     4141 gcgatgatac tggcggcagg acgcggcgag cgtatgcgcc ctttgaccga taccactccg
     4201 aagccgctgc tcgatgtggc gggtaagcct ctaatcggtt ggcacctatg ccgtctgaag
     4261 caggcggggt ttaccgaaat cgtcatcaac cacgcttggc tgggccggca gatagaagat
     4321 gctttgggcg acggctcggc ttatggcgtg aacatcgcct attcgcccga acccgcaggc
     4381 ggtttggaaa cggcaggcgg catcgcgcag gcattgccgc tgttgggcga taccccgttt
     4441 ttggtcgtca atggcgacgt gctgaccgac atcgatttta ccgccgcgtt tcagacggca
     4501 tcgtccctgc cggaacatat ttccgcccat ctgtggctgg tggaaaatcc gccgcaccat
     4561 cctgacggcg atttttccct gctgccggac ggcagcgtac agtcggaagt atccggcggc
     4621 aacggattga catttagcgg cgtgggcatt taccgtcctg aaatgtttga cggaatcgaa
     4681 gcgggcagtg tggcgaaact cgcgcccgta ttgcgtggcg aaatgcggca aaaccgcgtg
     4741 agcggtcaga agcatacggg cttgtggctg gatgtcggca cggtatgccg tctgaaagag
     4801 gctcaagccc ttgcaggggc ttggaagtga aataaaacgg tggcttgccg agccggctgc
     4861 cgaacggttc cgaccggatg tccggcatat tttcgtggag gcgtgaaaat agggcgttcg
     4921 gggctttccg tccaacaaat gccatctgaa gccgacccct gtcggatgcg gcggtttgcc
     4981 gtcggcgcgg tgtcggattt tattgggtaa ctttgttcag accgccgccc gtcagattgt
     5041 caaaacccgg tttcaggcgg tatggcggat tcggtttaac gtttcaacgc cagcaccaac
     5101 acgcccgccg ttaccagccc caagcctatc cattcctgcg tgttcgggcg ttcgtccaag
     5161 aaaaccaccg ccatcagcgc gaccaagacc aggctgaatt tgtcgatggg ggcgacttgc
     5221 gaggcgttgc ccagttgcag ggctttgaag taggcgagcc aggatgcgcc ggtagcaagg
     5281 ccggatagga taaggaatgt ccagttgcgc cccgtaaagc cgttcacacc ctgccatttg
     5341 ccggtgtagg tcaaaaacaa taccaaagcg gcgaggatca ccaaggtgcg gataaaggtg
     5401 gcgaaatccg aatcgatgcc ctgcaaaccc attttggcga aaatggcggt cagcgaggcg
     5461 aagcctgccg atgccaatgc ccaaaacagc catgcgttgc tgcccatgtt ttctccttgg
     5521 attgtgaaca atatgaacgg tatttttgtt gcagcgtcaa aaatttcact gcgggtttgg
     5581 tgcggataac gttataatat gcctgatatt attttcaatc cacctgtttg tcgcctgatg
     5641 ctttcagacg gtatgtccct cctcatttct aaaggaaaat catgagcttc aaaaccgatg
     5701 ccgaaatcgc ccagtcttcc accatgcgcc cgattggcga aattgccgcc aagctgggcc
     5761 tgaacgccga caacattgag ccttacggtc actacaaagc caaaatcaat cctgccgaag
     5821 cattcaagct gccgcaaaaa caaggccgtc tgattttggt taccgccatt aatccgactc
     5881 cggcgggcga gggcaaaacc accgtaacca tcggtttggc ggacgcattg cgccatatcg
     5941 gcaaagactc tgtgattgcc ctgcgcgaac cttctttggg tccggtgttc ggcgtgaaag
     6001 gcggcgcggc aggcggcggc tatgcccaag ttttgccgat ggaagacatc aacctgcact
     6061 tcaccggaga ttttcacgcc atcggtgcgg caaataatct gcttgccgcg atgctcgaca
     6121 accatatcta ccaaggcaac gagttggaca tcgaccccaa acgcgtgctg tggcggcgcg
     6181 tggtcgatat gaacgaccgc cagttgcgca acatcatcga cggcatgggt aaacctgttg
     6241 acggtgtgat gcgccccgac ggttttgata tcaccgttgc ctccgaagtg atggcggtat
     6301 tctgtctcgc caaagacatc ggcgatttga aagagcgttt gggcaacatc cttgtcgcct
     6361 acgccaaaga cggcagcccc gtttacgcca aagatttgaa ggcacacggc gcgatggcgg
     6421 ccctgctcaa agatgcgatt aagcctaact tggtgcaaac catcgaaggc acgcccgcct
     6481 tcgtacacgg cggcccgttc gccaacatcg cccacggctg caactccgta accgcaaccc
     6541 gtctggcgaa acaccttgcc gattacgccg taaccgaagc aggcttcggc gcggatttgg
     6601 gcgcggaaaa attctgcgac atcaaatgcc gccttgccgg tttgaaaccc gacgcagccg
     6661 tcgtcgtcgc caccgtccgc gctttgaaat acaacggcgg cgtggaacgt gccaacctcg
     6721 gcgaagaaaa cctcgacgct ttggcaaaag gcctgcccaa cctgctgaaa cacatttcca
     6781 acctgaaaaa cgtattcggc ctgcccgtcg tcgttgcgct caaccgcttc gtgtccgact
     6841 ccgatgccga gttggcgatg attgaaaaag cctgtgccga acacggcgtt gaagtttccc
     6901 tgaccgaagt gtggggcaaa ggtggtgcgg gcggcgcgga tttggcgcgc aaagtcgtca
     6961 acgccattga aagtcaaacc aataacttcg gtttcgccta cgatgtcgag ttgggcatca
     7021 aagacaaaat ccgtgcgatt gcccaaaaag tgtacggcgc ggaagatgtt gatttcagcg
     7081 cggaagcgtc tgccgaaatc gcttcactgg aaaaactggg cttggacaaa atgccgatct
     7141 gcatggcgaa aacccaatac tctttgagtg acaacgccaa actgttgggc tgccccgaag
     7201 gcttccgcat cgccgtgcgc ggcatcaccg tttccgccgg cgcgggcttc atcgtcgcgc
     7261 tgtgcggcaa tatgatgaag atgccgggtc tgccgaaagt tccggccgcc gagaaaatcg
     7321 atgtggacga acacggcgtg attcacggct tgttctgaac ggtttttgaa accagatgcc
     7381 gtctgaagcc gtttcagacg gcattttttc ggaacgcggg cggcggtatg ctataatccg
     7441 ccgttaaatt tctctatttt caggaaaaaa catgagtttg aaatgcggca tcgtcggttt
     7501 gcccaacgtc ggcaaatcca ccctctttaa cgcgctgacc cagtcgggta tcgaagcggc
     7561 aaactatcct ttctgtacca tcgaacccaa cgtcggcatc gtcgaagtgc ccgacccgcg
     7621 tatggtcgaa ttggcaaaaa tcgtcaatcc gcaaaaaatg cagcctgcca tcgtcgaatt
     7681 tgtcgatatt gccggtttgg tcgcgggcgc gagcaaaggc gagggcttgg gcaaccagtt
     7741 ccttgccaac atccgcgaaa ccgacgcgat tgtgaatgtc gtgcgctgct ttgacgacga
     7801 caatatcgtc cacgttgcag gccgcgtcga tccgattgcc gacatcgaaa ccatcggtac
     7861 agagttggcg cttgccgacc tggcaagtgt cgaaaaagcc atcgtccgcg aagaaaaacg
     7921 cgcccgctca ggtgacaaag acgcgcaaaa actggtcgat ctgtgcaaaa aactgttgcc
     7981 gcatctggac gaaggcaaac ccgtgcgttc cttcggtttg gacgcggaag aacgcgcgat
     8041 gctcaaaccg ctgttcctgc tgaccgccaa accggcgatg tatgtgggca acgtttccga
     8101 agacggtttt gaaaacaacc cgcacctcga ccgcctgaaa gaattggctg caaaagaaaa
     8161 cgcgccggtt gtggccgttt gcgccgcaat ggaaagcgaa atcgccgaat tggaagacga
     8221 cgaaaaagcc gagttcctcg ccgaaatggg cttggaagaa cccggcctca accgcctgat
     8281 ccgcgccggt tacgacctct tgggtctgca aacctatttc accgccggtg tgaaagaagt
     8341 ccgcgcgtgg acgatacaca aaggcgatac cgccccgcaa gccgccggcg tgattcatac
     8401 ggattttgaa cgcggcttca tccgcgccca agtgattgcc tacgatgatt ttgtctcgct
     8461 cggcggcgaa gccaaagcca aagaagccgg caaaatgcgt gtggaaggca aagaatacgt
     8521 cgtgcaggat ggggatgtga tgcacttttt gtttaacgtg taacccaaat gcggcaggtt
     8581 ttcagacggc tttgccgaaa atgccgtctg aagccggttt tgatgatttt cggcgtttcc
     8641 ataccgccgg aatgcggctg catcaaaata aaatcccgcc cgcatttccg atttgccctc
     8701 cccgattcct gcaaaacaaa ccgcctgccc cgctgttgca aaaagccgtc cggcattccg
     8761 aatatcccgt acaaaatgac ctttcagacg gcatttgcgc cgccgcgatg aaagcaaaaa
     8821 cattatgagc caagccttac cctaccgccc ggacatcgac acattgcgcg ccgccgccgt
     8881 cttgtccgtc atcgtgttcc atatcgaaaa aaattggctg ccgggcgggt ttctcggtgt
     8941 cgatatattc tttgtgattt caggcttttt gatgacgatg attcttcacc gcgaaatgtc
     9001 gggggggggg gggaggtttt ccctgaaggc attttatatc cgccgcatca agcggattct
     9061 gcccgcattt ttcgccgtat tggcggcaac gctgacaggc ggcttctttt tattcaccaa
     9121 agatgatttc tttcttttat ggaaatccgc gctgaccgcc ttgggtttcg cctccaacct
     9181 gtattttgca agggggaagg attatttcga tcccgcgcag gaagaaaagc ccttgctgca
     9241 catttggtct ttgtcggtcg aagaacaatt ttactttgtc tttccgatat tgctgctgct
     9301 tgtcgcccgc aaaagcccgc gcgtacagtt cggcttcctc gccgcattgt gcgccttaag
     9361 ccttgccgct tcctttatgc cttccgcgtt cgataaatat tacctgcccc acctgcgcgc
     9421 ctgcgaaatg ctggtcggat cgctgaccgc cgtgtggatg cgctgccggc aacctgccgt
     9481 cggcagacgc tgtgccgccg ccggcgcatt gtttgccgtg tgcatattgt caacctgcct
     9541 gttttcctat tcggaacaaa ccgcctattt cccgggcccc gccgctttga ttccctgtct
     9601 ggctgttgcc gcgctgattt atttcaatca ttacgaacac ccgcttaaaa aatttttcca
     9661 atggaaaatc accgttgccg ccggtttgat ttcctattcg ctttatctgt ggcattggcc
     9721 gatattggcc tttatgcgct atatcggccc ggacaacctg ccgccttatt cgccggcggc
     9781 agcgatcgtc ctgactctag cgttttccct gatttcttat cactgcatcg aaaagccgtt
     9841 taaaaaatgg aaaggttcgt tcgcacaatc cgttttatgg atttatgcct tgcctatgct
     9901 cattttgggg gcgggctcgt ttttcgcgat gaggctgccg tttatggcgc aatacgaccg
     9961 cttggggctg acgcgttcca acacctcctg ccacaacaat accggcaaac aatgcctatg
    10021 gggggatacg gaaaaacagc cggaactgct cattttgggc gactcccacg ccgaccatta
    10081 caaaacattc ttcgatgccg tggggaaaaa agaaaaatgg tccgccacta tggtttccgc
    10141 cgacgcttgc gcctatgtgg aaggctacgc gtcccgcgtg ttccaaaact gggccgcctg
    10201 ccgcgccgtt taccgctatg ccgaagaaca cctgccccgg tatccgaaag tggttttggc
    10261 gatgcgctgg ggcagccaga tgcccgaaaa cagccgctcc cttgcctacg atgccggttt
    10321 tttccaaaaa ttcgaccgta tgctgcacaa actctcatcc gaaaaacaag ccgtttacct
    10381 gatggcggac aaccttgcct cgtcttacaa cgtccagcgc gcctatatct tgtcttcacg
    10441 cataccgggt taccgccaaa ccctgcgccc ggacgacgaa agcaccctga aagccaatgc
    10501 acgcatcagg gaattggcag ccaaataccc caacgtctat attattgatg ccgccgccta
    10561 tatccccgca gattttcaaa tcggcggatt gccggtttac tcggacacag accacatcaa
    10621 cccttacggc ggcacggaat tggcaaagcg tttttccgaa aaacaacggt ttctcaatac
    10681 gcgccataac cattgattcg cttaaatttg ttacaatcgg cggtttgcaa aaacgctaat
    10741 ttttttgaaa gagaccgatg agcgtcatcc aagacctgca atcgcgcgac cttatcgcgc
    10801 aaacgaccga catcgaagcc ttagacgctt tgttgaacga acaaaaaatc gccctttact
    10861 gcggtttcga tccgaccgcc gacagcctgc acatcggaca cctgttgccc gtattggcat
    10921 tgcgccgctt ccaacaggcg gggcatacgc cgattgcttt ggtgggcggc gcgaccggta
    10981 tgatcggcga ccccagcttc aaagccgccg aacgcagctt gaattccgcc gaaactgttg
    11041 ccggctgggt ggaaagtatc cgcaaccaat taaccccttt cttgagcttt gaaggcggaa
    11101 atgccgccat tatggcgaac aacgccgact ggttcggcaa aatgaactgc ctcgacttcc
    11161 tgcgcgacat cggcaagcat ttctccgtca acgccatgct gaacaaagaa tccgtcaaac
    11221 agcgcattga ccgcgacggc gcaggcattt ccttcaccga gttcgcctac tccctgctgc
    11281 aaggctacga cttcgccgag ttgaacaaac gctacggcgc ggttttggaa atcggcggct
    11341 ccgaccaatg gggcaacatc accgccggta tcgacctgac ccgccgcctg aatcaaaaac
    11401 aagtgttcgg cctgaccctg cctttggtta ccaaatcaga cggcaccaaa ttcggcaaaa
    11461 ccgaaggcgg cgcagtatgg ctgaacgcga aaaaaacctc gccctatcag ttctaccaat
    11521 tctggctgaa agtcgccgat gccgatgtgt ataaattcct gaaatacttt accttcctgt
    11581 ccatcgaaga aatcgatgcc gtcgaagcca aagaccaggc aagcggcagc aagcccgaag
    11641 cgcaacgcat cctcgccgaa gaaatgaccc gcctgattca cggcgaagaa gcccttgccg
    11701 ccgcgcagcg catttccgaa agcctgtttg ccgaagacca aagcagcctg accgaaagcg
    11761 acttcgagca gctcgccctc gacggcctgc ctgcatttga agtttcagac ggcatcaatg
    11821 tcgtcgaagc cttggtaaaa acaggtttgg catcctccaa caaagaagcg cgcggctttg
    11881 tgaacagcaa agcggttttg ctcaacggca aacctgccga agccaacaac cccaaccacg
    11941 ccgccgaacg ccccgacgat gcctgcctgc tgaccgacga acacaaacgt ttcggcaaat
    12001 acaccatcct tcggcgcggc aaacgcaacc acgcgctttt ggtttggaaa taatccgatt
    12061 gccgcagaaa tgccgtcatt cccgcgcagg cgggaatgac ggcggagggt tgtttgtctc
    12121 ggtttacctg gttaaaaaag aacgattttc actgatgttg catcaggttt ggggcgatgt
    12181 ttcaacacat agcaccgcgc ctgctgcgcg ctttatgcgt ttggcgcgtt cggctgcggg
    12241 aaatttgcct acttttcccg cgtcgggcgg gcgtaacggg cggcacactg tctataaacc
    12301 gcaataccgt ttacaatgac cgcctgtttc accacatacc cgaacgcaac aatgaaaatc
    12361 aggctggggc ggcacaacgc gcccgacttt ccgcacgggg ccgccgtaac cataggcaat
    12421 tttgacggcg tacacctcgg acacaaacac atcctccaaa aactccgcct cgaagccgat
    12481 gcgcgcggat tacccgtcgt gaccgtcgtt ttcgaacccc aacccaaaga atttttcgca
    12541 ctccgcaccg gcaggatgcc accgtgtcgg atcagccccc tgcgcaccaa gctcgaatta
    12601 ttggaaggca caggctgtgt cgatgccgtc tgggttttgc gtttcgatca aaatttttcc
    12661 gaaatatccg cgcaagggtt tatcgaccgc ctgctgcgtc aaaccttgaa tacgcgttat
    12721 ttgctcgtcg gcgacgattt ccgtttcggc gcggggcggg aaggctgttt tgaacttttg
    12781 gcacaacagc ccgatatgca gaccgagcgt acgccttccg tcatcgtcga agacatccgc
    12841 accagcagta ccgccgtccg ccaagccctt tcagacggca accttgccta tgcgaaaaaa
    12901 cttttgggac acgactacgt tttggggggc agggtggtgc acggcagaaa actcggacgc
    12961 accttaaacg ccccgactgc caacatccgc ctgccccgcc accgttatgc actcggcggc
    13021 gtgttcgtcg tcgaagcgga cggcacattc ggcacgcggc gcggcgtggc gagcttcggc
    13081 ttcaatccca ccgttgatag cggctgttct caaaagcttg aagtccacct gttcgacttt
    13141 caaggcgacc tgtacggaca acggctgaat gtccgcttcc tgcacaaact gcgcgatgag
    13201 gaaaagtttg acggtatgga agaactgaaa aggcagattg aagccgatat ggaagccgca
    13261 aagtgttggt agaaaaacct tatacaaacc atccgattgg gctacaatca atcttttaac
    13321 ttttcagacg gcataggggt ttcccgttgt gaaatactgt ttgaggcgca atgccgtctg
    13381 aaccgaaata ttgtaacaat agagattaaa aaatgaccga ttacagcaaa accgtcaacc
    13441 tgctcgagag cccgtttcca atgcgcggca atcttgccaa gcgcgagcct gcatggctga
    13501 aaagctggta cgagcaaaaa cgctaccaaa aactgcgcga aatcgccaaa ggccgtccga
    13561 aattcattct gcacgacggc ccgccgtatg ccaacggcga catccacatc ggtcatgccg
    13621 tcaataaaat tctcaaagac atcattatcc gcagcaaaac ccaagccggt tttgacgcgc
    13681 cttatgtgcc gggttgggac tgccacggcc tgcccatcga agtgatggta gaaaaactgc
    13741 acggcaaaga tatgcccaaa gcacgtttcc gcgaattgtg ccgcgaatac gccgccgaac
    13801 agattgcccg tcagaaaaaa gactttatcc gcttgggcgt gttgggcgac tgggaccatc
    13861 cttacctgac tatggatttc aaaaccgaag ccgataccgt gcgtatgctc ggcgaaatct
    13921 acaaatccgg ctatctctac cggggtgcga aaccggttca attctgcttg gactgcggtt
    13981 cttcattggc cgaagcggaa gtggaataca aagacaaagt atcgcctgcg attgacgttg
    14041 cctatccgtt taaagacacc gccgcgcttg ccgccgcatt cggtttggca ggtatcgaag
    14101 gcaaagcgtt tgccgtcatc tggacgacca cgccttggac gctgccggcg agccaagccg
    14161 tatctgtcgg cgcggacgtg gtgtatcaat taatcgatac gcccaaaggc aaattggtgc
    14221 tggcgaaaga tttggcggaa gacgctttga aacgctacgg cttttcagac ggcatcgcca
    14281 tccttgccga aaccaccggc gacaagctgg aaaacctgca tatgaaccat ccgtttttgg
    14341 aacgcgatat tcctatgctc aacggcgacc acgttactac cgatgccggt accggcttgg
    14401 tacacaccgc ccccgcgcac ggtttggaag actacgccgt ctgcaataaa tacggcatcg
    14461 agctttacaa ccccgtcaac gccgaaggcc gatacatcgg cgaaacgccg cgtgtcgccg
    14521 gtatgcgcgt ttgggaggcg aaccccgtca tcctgcaatg gttggaagaa accggcaacc
    14581 ttttggcaag cagtaaaatc gaacacagct acgcccactg ctggcggcac aaaacgccgc
    14641 tgatttaccg cgcgacaggt caatggtttg tcggtatgga caaagccggt gccgacggca
    14701 aaaccctgcg cgacaaagcc atcaaggccg tggacgacac cgaattcttc ccgtcttggg
    14761 gtcgcgcgcg tttggaagcc atgattgaag gtcgtcctga ctgggtggtt tcacgccaac
    14821 gctattgggg cacgccgatg actttctttg ttcacaaaga aacgggcgag ctgcatccga
    14881 actctgccga acttttggaa aaagttgccc tgaaaatcga agaaaaaggc atcgaagcgt
    14941 ggttctccct cgataagagc gaactcttga gcgcggaaga ttgcgaaaat tacgataaac
    15001 tttctgacac aatggacgta tggttcgact ctggctcgac ccattattcc gttgtgaaac
    15061 aacgcgaaga attggaatgg ccggctgatt tgtatctcga aggcagcgac caacaccgcg
    15121 gctggtttca atcgtccatg ctgaccggct gcgcctcatc aatgggtcgc gcaccgtata
    15181 aacagctgct gacccacggt tttgttgtag acggcgaagg caaaaaaatg tcgaaatcca
    15241 tcggcaacgt cgtcgcgccg caagaagtct ataacgaatt cggcgcggac atcctgcgtc
    15301 tgtgggcggc atctaccgat tacagcggcg aattggcgat ttctaaagaa atccttaagc
    15361 gcgtaaccga aagctaccgc cgtatccgca ataccttgag ctttttgttt gccaacttga
    15421 gcgactttaa tccgattgaa gatgccgtgc aacaggcgga tatggtggaa atcgaccgct
    15481 atgccctggt gttggcgcgt cggctgcaag agcgtctggc aggcgattac tatccgcgtt
    15541 atgccttcca ttttgccgtg aaagaaatgg tttctttctg ctcggaagac ttgggcgcgt
    15601 tctacctcga tatcctgaaa gaccgcctct acaccaccaa agccgacagc cgcgcccgcc
    15661 gcagcgcgca aaccgccctg taccacatca cgcgcagcct ggttctcttg attgcaccga
    15721 ttttgtgctt taccggcgaa gaagcgtggg acatcatcgg cggcggcgaa gaagacagcg
    15781 tcctcttcca tacttggcac gagttcccga ccatcaacga aaaaaccgaa gccgaactgg
    15841 tgaaaaaatg gacggcaatc cgcgaagccc gcgaagcggt aaccgccgcc atcgagcctt
    15901 tgcgcgccga caaaaccgtc ggttcgtcct tgcaagccga agccgaaatc accgcgccgg
    15961 aagaaatggc cggctacctg aacgccttgg gcgaagaatt gcgctttgcc atgctggtgt
    16021 ctaaagcaga agtgaaagta ggcagcgaac ttgccgttgc cgctaaagcc agtgatggtg
    16081 aaaaatgcga acgctgctgg cactacaccc gcgatgtggg cgcggttgca ggctatgaaa
    16141 ccgtctgcaa acgctgtgca gagaatgtcg gcggagaagg cgaaacgcgc cattacgcct
    16201 gataaagttt gagcaaatgc cgtctgaaac cgccgaacag catttcagac ggcatttttt
    16261 gtgccgcgat ttgtctttat aacggcggag gggtttcaag attgcggtgt tgtcggaatg
    16321 caactgaacc gtcattccca cgaaagtggg aatctagaat ctcgaggttt cagtcatttc
    16381 cgatagattc ccgccgcgtc gtgggtctgg attcccgcct gtgcgggaat gacggatttc
    16441 gagattgcgg tgttgtcgga acgcaactga accgtcattc ccacgaaagt gggaatctag
    16501 aatctcgggg tttcagtcat ttccgataga ttcccgccgc gtcaggggtc tggattcccg
    16561 cctgtgcggg aatgacgg
//
LOCUS       DAWFIE010000044        16280 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWFIE010000044 DAWFIE010000000
VERSION     DAWFIE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16280
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.044"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..114
                     /locus_tag="WGQ71_001278"
     CDS             <1..114
                     /locus_tag="WGQ71_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0707286.1"
                     /translation="PFFISNDTDLALFKTTKWGICRAIADAIAMELGAAKV"
     gene            111..284
                     /locus_tag="WGQ71_001279"
     CDS             111..284
                     /locus_tag="WGQ71_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707287.1"
                     /translation="MKKSLIALALSVLKPQMPEFEIKPASIGYLKQHPSMRLGKSGVA
                     AAKRAARKRKNRR"
     gene            287..451
                     /locus_tag="WGQ71_001280"
     CDS             287..451
                     /locus_tag="WGQ71_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707288.1"
                     /translation="MGQVEFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            454..690
                     /locus_tag="WGQ71_001281"
     CDS             454..690
                     /locus_tag="WGQ71_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707289.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            662..1084
                     /locus_tag="WGQ71_001282"
     CDS             662..1084
                     /locus_tag="WGQ71_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707290.1"
                     /translation="MNLVKLLANNWQPIAIIALIGTGLAVSHHQGYYKSAFAKQQAVI
                     DKMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENK
                     KEIENVLTQDRKNAGGGCIDGFGHHGLQLYKRALGYGN"
     gene            981..1286
                     /locus_tag="WGQ71_001283"
     CDS             981..1286
                     /locus_tag="WGQ71_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707291.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTSAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(1331..1501)
                     /locus_tag="WGQ71_001284"
     CDS             complement(1331..1501)
                     /locus_tag="WGQ71_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ0707292.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            1514..1732
                     /locus_tag="WGQ71_001285"
     CDS             1514..1732
                     /locus_tag="WGQ71_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707293.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            1729..2055
                     /locus_tag="WGQ71_001286"
     CDS             1729..2055
                     /locus_tag="WGQ71_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707294.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFCVEFSATAPADVIGILRKYYSVFSDGQKVEAASRHPECHAVRIV
                     FGDVPV"
     gene            2067..2333
                     /locus_tag="WGQ71_001287"
     CDS             2067..2333
                     /locus_tag="WGQ71_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707295.1"
                     /translation="MDFEFGFKTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            2330..2527
                     /locus_tag="WGQ71_001288"
     CDS             2330..2527
                     /locus_tag="WGQ71_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707296.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            2530..3036
                     /locus_tag="WGQ71_001289"
     CDS             2530..3036
                     /locus_tag="WGQ71_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ0707297.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQATARAWKRSDK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(3069..3545)
                     /locus_tag="WGQ71_001290"
     CDS             complement(3069..3545)
                     /locus_tag="WGQ71_001290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707298.1"
                     /translation="MTGFISNAFIGLKEAGSLLKGFNQLKTEAEVAQKTIELNQIIAG
                     IQTDLFEAQSSYTAAISRIDELEKELVAVKNWSTEKQRYQLYELVPGSFVYRLKAEMA
                     NSEPIHDICPQCYEQGVKSILQFNGYEGAFHKYSCSRCQTIILGERLKSDIEVFSG"
     gene            3557..5179
                     /gene="terL"
                     /locus_tag="WGQ71_001291"
     CDS             3557..5179
                     /gene="terL"
                     /locus_tag="WGQ71_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ0707299.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHEFLFSRLPEIIHSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAVTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            5179..6747
                     /locus_tag="WGQ71_001292"
     CDS             5179..6747
                     /locus_tag="WGQ71_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ0707300.1"
                     /translation="MAKKDNKTKIQKPEAALQTDAAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLMMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     NGMTSAGNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEIRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPHADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPVNRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            6734..8029
                     /locus_tag="WGQ71_001293"
     CDS             6734..8029
                     /locus_tag="WGQ71_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ0707301.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDSYPTTAYKAPDGSLYTADKGFGYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKEMQELAGM
                     TRATVWLSDDTLVKQVDSREGQNFDDSYYAFLPDMLQNPEHVIRDNRELIFTARYKGS
                     ALWAVLKYIKEVDEIYLQSYRISNDKEIAKFMAKKKVLK"
     gene            8139..8555
                     /locus_tag="WGQ71_001294"
     CDS             8139..8555
                     /locus_tag="WGQ71_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ0707302.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKLLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            8786..9847
                     /locus_tag="WGQ71_001295"
     CDS             8786..9847
                     /locus_tag="WGQ71_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707303.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFDLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAKGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            9897..10235
                     /locus_tag="WGQ71_001296"
                     /pseudo
     CDS             9897..10235
                     /locus_tag="WGQ71_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10309..10920
                     /locus_tag="WGQ71_001297"
     CDS             10309..10920
                     /locus_tag="WGQ71_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0707304.1"
                     /translation="MAAPAGVATECRLTVQGGTDRESDASLLARLLEIIRRPPAGGNR
                     YDYKNWALSVDGVTSAYVYPLRRGLGTVDIAITSADGVSSEETVRRVQAYIDEMRPVT
                     AKNALVLKPTVTAVPVTVQVKLDGIDLDEAKRRIRTALKEYFDTLIPGDGLTVSQIEA
                     AISNVDGVIDRRLTAPTANRAADTVNRIEWFKAGAINVTEMPS"
     gene            10917..11477
                     /locus_tag="WGQ71_001298"
     CDS             10917..11477
                     /locus_tag="WGQ71_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ0707305.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQHRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            11488..13464
                     /locus_tag="WGQ71_001299"
     CDS             11488..13464
                     /locus_tag="WGQ71_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ0707306.1"
                     /translation="MHPIETPDKTFHDGDGVSELDTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGNQEIGGQKTFTAQTQFPSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHSANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPVENTACHIQVIAGTQPGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLRADGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDAA
                     ALGYEDRNERQRSALVSTWTDENLNDNGFLTPRSDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQNLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRRDGLLEALQD"
     gene            complement(13461..13673)
                     /locus_tag="WGQ71_001300"
     CDS             complement(13461..13673)
                     /locus_tag="WGQ71_001300"
                     /inference="COORDINATES: protein motif:HMM:NF016757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PT domain-containing protein"
                     /protein_id="HEZ0707307.1"
                     /translation="MDRRAEAHLYSPSLQPISTTHLYSPSLQPSLQPSLQPSLQPSLQ
                     PSLQPSLQPSLQPSLQPSLQPVSTAR"
     gene            complement(14241..14543)
                     /locus_tag="WGQ71_001301"
     CDS             complement(14241..14543)
                     /locus_tag="WGQ71_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ0707308.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(14515..14802)
                     /locus_tag="WGQ71_001302"
     CDS             complement(14515..14802)
                     /locus_tag="WGQ71_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ0707309.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVCFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            14882..15487
                     /locus_tag="WGQ71_001303"
     CDS             14882..15487
                     /locus_tag="WGQ71_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707310.1"
                     /translation="MTIYFKNGFYDDTLGGISEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAATRGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            15466..15621
                     /locus_tag="WGQ71_001304"
     CDS             15466..15621
                     /locus_tag="WGQ71_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707311.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTR"
     gene            15759..15968
                     /locus_tag="WGQ71_001305"
     CDS             15759..15968
                     /locus_tag="WGQ71_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707312.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
BASE COUNT         4170 a         4284 c         4484 g         3342 t
ORIGIN      
        1 ccgtttttta tcagcaacga cactgatttg gccttgttta agacgaccaa atggggcatc
       61 tgccgcgcga ttgcggacgc gattgcgatg gaattgggag cggcgaaggt atgaaaaagt
      121 ctttgattgc tttggctctg tctgtcttga aaccgcagat gcctgaattt gagattaagc
      181 ctgccagtat tggctatttg aaacaacatc cgtctatgcg cctgggcaag tcgggcgtag
      241 cggctgcgaa acgcgcggcg cgtaaacgca agaatcgtcg ttaatcatgg gacaggttga
      301 gttttacgaa aagatgattg ggctgtggtc ggccaaaagc cgtgaggcaa gtgaacaggc
      361 agacttggcg gcgtttgaat ttgcggaggg cgaactggcc aattatcagg aaatgctgaa
      421 acggcacctg caaaccaaaa gtgtggaata gcaatgcgta ttttggatat ttttaaaaac
      481 cccgcgacag gcaatgtgtc gcactcgaaa ctgtgggcaa acgttgcctg cgcggcgggg
      541 acgtttaagt ttgtgatgct gcccgatccg tcggcggaga tttgggcggt gtatttgggc
      601 attgtcggcg gctatgcggt ggcgcgctcg tttgtcagcg tcaaacgtca ggaggtcgag
      661 aatgaatctc gtgaaactgc tggcgaataa ctggcaacca attgccatca tcgcacttat
      721 cggcacgggt ttggcggtgt cgcaccatca aggctattat aagtcggcgt ttgcgaagca
      781 gcaggcggtc attgacaaga tggaaaaaga caaagcgcaa gccctgctgt tgtcggctca
      841 aaactacgcc cgcgagctgg aacaggcgcg tgcggaagct aaaaaatatg aagtcaaggc
      901 gcacgccgtc ggcatggctt tggcgaaaaa acaggcggaa gtcagccgtc tgaaaacgga
      961 aaataaaaag gaaatcgaaa atgtccttac tcaagaccgt aaaaatgcag gcggcggttg
     1021 tattgacggc tttggccatc acggcttgca gctctacaag cgcgccctcg gctacggaaa
     1081 ttaaggttgt cgaaaaggcg gtcatgccga caccgcctgc cgcattgatg gtcgcgccgg
     1141 tacgcccaaa tccgccgaaa gacggcaaga cggccacgct gttggaacac gccgccgagt
     1201 ttggcggcta tgtggcggag ttggaaaatc aaaatcaggc ttggcgcgac tgggcgggca
     1261 atcactcccg caaagtcgga aactgacaaa aaagcccgcg tagggcgcgg gctgagggtg
     1321 aaagcggatt ttatacctct tttacagggg tagcggcggt agtgcttttc agcaaatcga
     1381 ctgcgtgctg acagttttgc ttgctggtgt agccttcgcc ctgagcgatg atttcatggt
     1441 tggctgcttt caaacgccaa cggtattcgc cttttgcgtc tttatagatt tcaaaataca
     1501 taaggtttct cctatgaatg agtacacgtt ttcttaccgc tttaacggca agtcctggtc
     1561 attgagcatt tgggcggaca accctgaaga agccagggcg aaatttcggg ctgcacgaga
     1621 aaatgcgcac tatgacggcg aagttgtagc aaaggtttat acatttgtaa atatttcgtg
     1681 ggttaagaaa ttgtacaagc ggacaaaata tttaatgggt atcaaagaat gacctaccgt
     1741 gaattagttg aacgtcagtt ggctgtgcgc catgccgatt tggaattggg cttaagccgc
     1801 gcacgcgagc aagagccgtt tgtcattcat gtttccgatc tgttggataa ggcaggcatt
     1861 gagtacgcgg tacgcatgga taaggatttt cagacgacct tttgtgtgga gttttccgcg
     1921 accgcccctg ctgatgtgat tggtattttg cggaaatatt actcagtctt ttcagacggc
     1981 caaaaggtcg aggcggcgag tcgtcatccc gaatgccacg cggtccgtat cgtatttggc
     2041 gatgtgccgg tttaaagggg ttttaaatgg actttgaatt tggttttaaa accctgtggc
     2101 cgattgcgac ggcggcattt tggttttggg tcaatggcat ttcaggccgt ctgaaagagg
     2161 cggacaagcg tatcgatgac cttaaagagg agctgcacgc ggtcaagctc tcttatcaca
     2221 ccaagcaaga tgccaaggca gaccgcgaca atatcgcagc ttctttggga cgcatcgaaa
     2281 acaagttgga aaaagtaaac gaaaaactgg acaggaaagc ggacaaatca tgagcgaccc
     2341 gattttggaa gccttggcgc gtattgaaaa caagactgat caaacgctga aaaatcagaa
     2401 ggaaatgcag gcggaaattg cgcaaatccg ccaagacacg aaacgcacgg ccattacatt
     2461 cggcgcactg ggcggcggcg tgattacggt cggctgggaa ttgcttaaag cgaaaatggg
     2521 actgtaatta tggctcaccc gcaagaaatc cgtgaaaagt tacgccggct ctatgtgagc
     2581 ggcgagcaaa ctttggaaac ggcggccttg atgtgcgaaa tcccgcaggc cactgcgcgt
     2641 gcgtggaaac gttcggataa ggaaaaaggc gacgactggg ataagatgcg cgccgcttac
     2701 actttggccg gcggcggtat tgaggacttg agccgtgcga tgttggccgg ttttatggtg
     2761 cagtacaaca gcacgatgac gatgctgcag gattcgagta ccgaagattt gccgccgtcc
     2821 gaccgtgcga agctgttggc cagcctggcc gatgcgttta cgaaaaccgt atccgccaat
     2881 gcccgtgtga tgccggaaac gtcaaaactg gcgacggctt tggaattgat tgagttcttg
     2941 atggcgtttg tgcaagaaaa acatcccaaa catttgcctg cctttgtgga ggtattggag
     3001 ccgtttgggg tggaagtgga gaagaagttt ggttaatagc cgtctatcaa tctcccataa
     3061 cgtgatcctc atcctgaaaa tacttcaata tctgacttta aacgctctcc caatattatg
     3121 gtttggcaac gggaacagga atatttatga aaagcacctt catacccatt gaattgaaga
     3181 atcgatttga caccctgctc gtaacattgg ggacaaatat catgaattgg ctcgctgttc
     3241 gccatctccg ctttcaatcg atagacgaaa gaaccaggaa caagttcata caattggtag
     3301 cgttgcttct ctgtagacca attttttact gccaccagtt ctttttccaa ctcatctata
     3361 cggctaattg ccgccgtata agaagattgg gcttcaaata aatcagtttg aataccagca
     3421 atgatttgat taagttcgat ggttttttgc gctacctctg cctctgtttt gagctggtta
     3481 aaccctttta aaagtgatcc tgcttctttt aggcctataa atgcgttaga aatgaatcca
     3541 gtcattataa atactcatga aaacaaaaga attcctcaaa tcccttgccg aactggccgc
     3601 cagcctgcgc caagtcatcg aagcggaagt ggacggcttc gatgcgtcgc ccaaggctat
     3661 tgctgcacgc cgtgccaagg tgtttgaccc ggtaggcggt tacgagtatt tcgtcaacac
     3721 ctactttccg cattatatcc gctcccctga aaaatccgaa ctgcatgagt tcttattcag
     3781 ccgtctgccg gagattatcc actcccccaa aggggaaaat gaggcggtag gtgcgccgcg
     3841 tggcgagggt aagtcgacgc aggttactca gttgtttacg ctgtggtgta ttgtgacagg
     3901 ccaaaaacat tatgccgtta ttgtgatgga cagtattgac caggcatacc cgatgctcga
     3961 agccatcaag gcggaattgg aatttaatcc gcgcttgaaa accgactttc cggaagtatg
     4021 cgggcagggc cgtgtatggc aggccggtac gattgtgacg gccaatgacg ttaaagtcca
     4081 agtggccggt agcggtaaaa agctgcgcgg tttgcgtcac ggcccttacc gtcctgactt
     4141 aactgttttg gacgatattg agaatgacga gcaagtccgc aacccggaac agcgcgacaa
     4201 gctcaatgcg tggctaacta agactgtatt gcctttgggc ggggtcggcc agaaatacga
     4261 tgtgatatat atcggcacga ttttgcatta cgacagcgta cttaaccgca ctttgaataa
     4321 cccgttttgg cacggtatta agtttaaggc gatgaaacgc tggcccgacc gcatggattt
     4381 gtgggacaga tgggaggaac ttttccgcaa cgacggcgag acggtggccg aggcgtttta
     4441 tctcgccaac aaagacgaga tggagcgcgg cgcagtcact tcttgggcgg cgcgtggcgt
     4501 actcgcgctg atgaaaatcc gcgcccgcga cggtcatgcg acgtttgaca gcgaatatca
     4561 aaacgacccg gtcagcggcg aagatgcgcc gtttgccaag tcgatgaagt tttggaacga
     4621 cctgccgtcc gatttggtgt atttcggtgc gctcgacccg tcgctgggta aagcgggggc
     4681 gagccgtgac ccgtcggcga ttatcattgg cggttatcaa cgtgaaaccg gcaaactgta
     4741 tgtcgtggaa gctcagatta aaaaacgtct ccctgatttg attattgagg acgttatcag
     4801 attgcaccgt caatatcgtt gcaaactgtg gtttgttgag acggtgcagt ttcaggagtt
     4861 tttaaaagac gagctggtca agcgcagtgc ggcgcgtgga atacctgtcc cggcgcgggc
     4921 ggtcaagccg gtatcggaca agctcttgcg gattgagact ttgcagcctc acatggcgaa
     4981 cggtttgatt ctgttgaatg agagccaaca aacgctgata cagcagttcc gccattttcc
     5041 aaaggctgat catgatgatg gtcctgatgc cgtgcatatg ctctggtcgg gggcggtggc
     5101 gaattgtgtg ccgatagaat ggcaaagccc taccgataac gattttgatg acgagataaa
     5161 aagtaaatgg agccgataat ggcaaaaaag gacaataaaa ctaaaatcca aaagcccgag
     5221 gctgcattgc agacggatgc ggctcaaatt acggcgaccg gtcgggttat cgccgagcat
     5281 ccgtccaatt ttattacgcc gcaaaagatg cgggccctct tcgaggacgc agaaagcggc
     5341 gacatccgcg cccaacacga gcttttcgcg gacattgagg agcgcgacag cgacatcgcg
     5401 gcaaatatgg gtacgcgcaa acgcgcgctg ctgacgctca actggcgcgt cgccccgccg
     5461 cgaaatgcga cgcccgaaga agaaaagctg tccgaccaag cctacgaaat gatggacagc
     5521 ctgcctaccc ttgaagacct gatgatggat ttgatggacg ctgttgggca cggattttct
     5581 gcgttggagg tcgagtgggt attttcagac ggcctttacc taccccgaaa ctttatccac
     5641 cgcccgcaaa gctggttcaa atgggacaaa gacaacgggc tgctgctgcg tacccgcgaa
     5701 aatccggaag gcgaagcgtt gtggccgctg ggctgggtcg ttcataccca aaaatcgcgc
     5761 agcgtccagc aggcgcgcaa cgggcttttc cgcacgcttt cctggctgta tatgttcaaa
     5821 cactacgccg tccacgattt tgccgagttt ttggagctgt acggcatgcc catccgtatc
     5881 ggcaaatacg gcgcgggcgc aaccaaagag gaaaaaaaca ccctgcttcg agcggtggcg
     5941 gaaatcggtc acaacgcggc aggcatcatg ccagaaggta tggaaatcga gctgcacaac
     6001 gcggcaaacg gcatgacttc cgccggcaat ccgtttttgc agatggccga ctggtgcgaa
     6061 aaatcggcgg cgcggctgat tttggggcaa acgctaacca gcggtgcgga cggaaaatcc
     6121 agcaccaacg cgctgggcaa tatccacaac gagatacgcc gcgatttgct ggtgtcggac
     6181 gcaaaacagg tggcgcaaac catcacaagc caaatcatcg gaccgttcct gcaaatcaac
     6241 tatccccatg ccgacccaaa ccgcgtgccg aaatttgaat ttgacacgcg cgagccgaaa
     6301 gacatcgcgg tctttgccga cgctatcccg aaactggtgg atgtcggcgt acaaatcccc
     6361 gaaagctggg tgcgcgacaa actggtcatt ccagatgtgc aggagggtga ggctgtgttg
     6421 gtgcggcagg taccggacaa tccggtaaac agaactgcat tggcggcttt atctgcccac
     6481 accgtaccat ctaaggctac gggcaggcat caggaaatat tggacggcgc gttggatgac
     6541 gcgctggttg agcccgattt caattctcag ctcaacccga tggtgcgtca ggcggttgcc
     6601 gcacttaatg cttgcaacag ctacgaggag gcagatgccg cactgaatgc gctttatccg
     6661 aatttggaca acgcgaaact gcgtacctat atgcagcagg ccttgtttat cagcgatatt
     6721 ttgggacaag accatgcccg cgcctgattt gggatttgcc ttaagtctgc cgccaaaaaa
     6781 ggcaatcgag tggctggaaa gtaaaaaggt tacggcggag agctaccgca atctgacagc
     6841 ctccgaaatt gccaaagtct atacgattgc ccgcatgacc gacttggata tgctcaacga
     6901 catcaaaact tcgatggttg aatcggcaaa aagtggacag tcgtttgacg attggcgaaa
     6961 aggtatcttg aatctgctca gcaacaaggg ctggctgcat ccgaacgggc ataacggtaa
     7021 ggatatcatc gacccagcca ccggcgaggt attcggttcg ccgcggaggt tggagacgat
     7081 ttaccgtacc aacatgcaaa ctgcctacaa cgccggtcaa tatcaaggat atatggcaaa
     7141 tattgatgca cgaccttatt ggatgtatga cgcggtaggc gacagccgca cccgtccggc
     7201 gcattcggca atagacgggc tggtgtaccg ctacgacgac ccgttttggg caacgtttta
     7261 cccgcccaac ggctacaact gccgctgctc ggtcatcgcg ctgtcggagc gggatgtgga
     7321 acgccagggg cggattgttg ggcaaagcac ggcggacaat ctggtcgaga cccataaaat
     7381 ctacaacaaa aaaggcgaca gctacccgac gactgcctac aaagccccgg acggcagcct
     7441 ctacacagct gacaaaggct tcggctacaa cgccgggcga atgaactacc gccccgattt
     7501 agacaagtac gaccgtgcgt tggcgcatca atttgccaaa gcggaaatgg gtggtgcgga
     7561 ttttaaaacc agctttaaac agcttgaaaa agagttttat gaagtcaagc aacgtttgga
     7621 tattgatggc aagcccgata aagagcagaa aatcaaaatc cgaaatgcgc tatcaagaca
     7681 gcttaaattt gctgcgggtg tattgagcaa ggaaatgcaa gaactggcag gtatgactcg
     7741 agcgacggtg tggctgtctg atgatacatt ggtcaaacag gtggacagcc gtgaggggca
     7801 gaatttcgat gactcctact atgctttttt gccggatatg ctgcaaaacc ctgaacatgt
     7861 catccgcgac aatcgtgaat tgattttcac agctcgctat aaaggctcgg cattgtgggc
     7921 agttttaaaa tatattaagg aggtggatga gatttatcta cagtcgtacc gaatcagtaa
     7981 cgacaaagag attgccaaat ttatggcgaa gaagaaagta ttgaaataga cgttgggcaa
     8041 ggctcgaaat cacttgcaca cgctctcgga cgccctaacg ggcaggctgc ggtatcgagg
     8101 ttatcaccgc ttttccaacg tctgtacgga ataataccat gattgatgtc aaaatagaca
     8161 atatctttgt cgtcctaaac caaatcgagc ggcttggcaa cgggatcgaa aaccgctacc
     8221 tgctgatgcg ccgactgtcc gaaaccatgc acacggcggt caagctcaat ttccgctacg
     8281 caggccgtcc gaaatggttg gggctaaaat accgcgacgg caagctgctt tcggattcgg
     8341 gtcgtctgaa agacagtttt tccacactgt cagacaacga taccgccctt gtcggtacga
     8401 atatcgtcta tgccgccatc cacaacttcg gcggtatggc ggggcgcaac cgcaaagttc
     8461 ggattccgca acgggaattt ttgacgctga cggacgacga caaacaggct ttgatggacg
     8521 atgtgcagga ttatttttcg ggtctgatac cgtgaattta taaaaccctc aaaaacgcgc
     8581 tttttagcgc gtttttttat gcgggtaata caaacccctg cccaagatat aaaaatcaat
     8641 cctagactct tctaaaaagc ccctgaaaac gattaattgt gtatcgcgcg gacaggtttt
     8701 aaaaaaatgg cgggagggtt tgaagcacgc ctactctttg ttgttttttc aaataggcaa
     8761 aatgaccgta ttgagagagg tacacatgtc caaaaatgca caaaaaaccc tacttgccgt
     8821 gtgcagtttc gaggtgcagc caaaagacgg gcgaatccaa ctgctgccat atggcgaatt
     8881 tcgcgcagta gacggtcgtc cgactgatgt ccctgcgtgg tatctgaccg aagaaaacgg
     8941 tcatgatgtc gcgttgttgg ccaacagctc gcgcaatcag ttggttgtcg attatgaaca
     9001 ccagacgctc tacaaagaga aaaacggaca acctgcacct gccgccggtt ggatgcgttg
     9061 gctggagttc acgcctaaag gcatgtttgc cgaagtggag tggacggaca aggcggctgc
     9121 ggcaattgcc gcaaaagagt atcgctacat ctctgctgtg ttttcctatg acacaaaggg
     9181 atatgtaagc aaaatttttc acgccgcgct gacaaatttc cccgcgttgg acggtatgga
     9241 cgaggtgctg gcggcagcgt cggcgcaaat tttaaaaccg gaaacggagc aaaaccctat
     9301 gaaagagttg ttacagcaac tgttcgacct gcctgatgcg ggcgaagaag aactgaaggc
     9361 ggcattgtcc gcgctcgtgg aagccaagcc gaaagacgtg gcattgtctg ccgacgtgtt
     9421 cgcgcagctg gcggaaaaag acagccgcat cgcggcattg acggcgcaaa ccgccaagcc
     9481 tgatttgact aaatacgcgc ctatctcagt ggttcaagag ctgcaaagca aagtcgccgc
     9541 gctgactgcc aagcaggaag cagacaaagg caacgaattg attaccgccg cgctgacttc
     9601 aggcaaattg ctgcctgctc agaaggagtg ggcaaaaggc gtattgaaac agccgggcgg
     9661 cttggcattt ttgaccggct ttattgaaaa cgcccagccg gtcgctgcac tggcaggctc
     9721 gcaaacgggc ggcaaagcac ccgacgaacg cgtcgccgca ctgactgcgg aagaggcagc
     9781 cgcagcaaaa atgctgggca tgtccggcga agaatttgta aaaatcaaag aaagcgaagg
     9841 taagtaatgg acaaatcagc gattttgacc gcaatcacgg cagcattccg caaagaattt
     9901 caaacggggg cctgcaaaac cgcctgaacc ggttaaccgc caaacaggtg cagccggtag
     9961 cgcaggcggt gcgcctgtta tccacgtcat cgctgttgtc ggtggcaacg gcattaatcg
    10021 aggcgcatgg cgaagagatg actgcgcccg atttgattga ggttaaccgc gccatgcgcc
    10081 gccgtatgca ggccgagatt gccgccttgc gggcggtgca gacggctgct gccgagtctg
    10141 gtgggctgac gaccaacgcc gtgtataccg aggcttacca aacggcagaa tccctgcgcg
    10201 cggcggcagg ccgtctgaat gcgttggttg cggcggcacg gcggaaatac cggcaatcgc
    10261 cgacgagccg ggcgcggccg ccaatgtggc gacggcgagg cgcaactgat ggccgccccc
    10321 gccggtgtgg ccaccgaatg ccgccttacc gtacaaggcg gcaccgaccg agaaagcgat
    10381 gcctcactgc tggcgcgtct gttggaaatc atccgccgac cgcccgcagg cggcaaccgt
    10441 tacgactata aaaactgggc gttgagtgtt gacggcgtaa ccagcgcata tgtttatccg
    10501 ctgcgccgcg gcttgggtac ggtggatatt gccattacct ccgccgacgg tgtgtcgtcg
    10561 gaagaaactg tgcgccgcgt acaggcttat atcgacgaga tgcgcccggt aacggcaaaa
    10621 aatgcgctgg tactcaagcc aaccgtaacg gcggtgcctg ttaccgtgca agtcaagctc
    10681 gacggtatcg acttggacga ggccaagcgc cgcatacgga cggccctaaa agaatatttc
    10741 gacaccctga tccccggcga cggcctgact gtgtcgcaaa tcgaggctgc tatcagcaat
    10801 gtggatggtg tgatcgaccg ccgtctgact gcgccgacgg ccaaccgtgc cgccgatacg
    10861 gttaaccgca tcgagtggtt taaagcgggc gcgattaatg taacggagat gccgtcatga
    10921 gctatcaaga catcttgcgg ggcctgttgc cccccgtgtc gtatgcccgc aatgccccgc
    10981 gtgtgcgggc gcaggcagaa atagacggcg cagcgctgga tgcggtggcg gaatcggctc
    11041 aaagcgttgc cgatgccgtc gacccgcgca gcgccggcca aatgctggcc gattgggagc
    11101 gcgtattagg tttggacggt acgggcaaaa accgccagca ccgtgtgttg gccgtcatgg
    11161 ccaagctaaa cgaaacaggc ggcttgagta ttccttattt tgtgcgtttg gccgaggcgg
    11221 cgggctatca aatccaaatc gacgaaccgc agccgttccg cgccggtgta aaccgcgccg
    11281 gcgaccgtct tgcgccgcag gaaatcatgt gggtgtggca cgttaacgtg cgcggcggca
    11341 acaaccgcat tacccgattc cgcgccggta tctcggcggc gggcgacagg ctgaccgatt
    11401 acagcgatgc cgtgatcgag agcctgttca accgcctcaa gcccgcccac accgctatcc
    11461 gatttaccta ccgataagga ctaaaccatg caccccatcg aaacccccga caagaccttc
    11521 cacgacggcg acggcgtgtc cgaattggac accatcctgc ccgcgtggtg gctcaaccaa
    11581 gtgcaatccg agctgctggc cgtgctgact gcggccggta tccagccgga taagtcgcag
    11641 cctaatcaat tactggcagc actgaataga ctggctgtag ttaccaccgg caaccaagaa
    11701 atcggcggcc aaaaaacctt tactgcccaa acccaattcc caagcggcat ccatttatcc
    11761 gccaaccaga cgaactggaa cggcggccac aaagcctaca tcggcgcgga tgccgacaac
    11821 gctcacatcg tcttcggcga cgacaccctc cgcctgcaca gcgcaaacaa ccgcatttcc
    11881 tacaacaacc acgacatctt ccacaaagcc aacaaaccgc gttttgccga agacatcgaa
    11941 ggcaaaccga acacactgtc cggatacggc atcggcaatt tcaaagtcga aacattccgg
    12001 ggcgatttga acaccctcaa aacagacggc atctattccc tgccgacggc ggtcggcagc
    12061 tccaacctgc ccgttgaaaa cactgcctgc catatccaag tcatcgccgg tacgcaaccc
    12121 ggctggtgca ggcagttggg ttatcccgcc tacacgtccg acgtgtacga acgccaccaa
    12181 acgagcagcg caaacgacaa ctggtccgca tggaaaaaac tcaattcaga cggcatcccc
    12241 gtcggcgcga tcgtatcctt tcccaaagcc gtccgaaacc ccgcaggcta tttgcgggca
    12301 gacggcacaa cctttgcgca aaacaccttc cccgaccttt accgcgcctt gggcaacagc
    12361 aaccgcctgc ccgatttaag ccgtaccgac atcggcatca ccgcgtggtt tccgtccgac
    12421 caaatcccga ccggctggct ggcgtttgac gacatccgca cgcgcgtaac cgaaaccgct
    12481 tatcccgagc tgtaccgtct gctgaccgga aaatacggca gcatccaaaa cgtcccgcag
    12541 gcggaagacc gctttatccg caacgcgggc aacagcttgg cagtcggaac gaagcaggaa
    12601 gacgaaatca aacggcacgt ccacaaagta ttttcacact ggacaaacca cacagacgcg
    12661 gcagccctcg gttacgaaga ccgcaacgaa aggcagagaa gcgcgctcgt atcgacttgg
    12721 acggacgaaa atttaaacga caacggcttt ttaaccccgc gctcggacag caaaatggcg
    12781 acaggcggcg acgaaaaccg ccccaaagcc ctggttttaa aactgtgcat caaagccgcc
    12841 gacaccttgg gcgaagccgt gttttggata aagtcccacg gcgaaaccat caacgcaggc
    12901 gcgctggacg cgggcacgct ggcgcaaaac ctccaagaca aagccgaccg cgcccacacc
    12961 cacaccgccg cccaaatcca agggctggac gaaaaaatca gcaccgccgt tgccgcgcaa
    13021 ttcacacgcc aaaccatcgg cggcgtggat attgtcagat tccccgacgg cacaatgata
    13081 cagaccggca gttacaggtt tacaagaagc ggcggcccca tcgaaaacga agtcgtcttc
    13141 cccgtcgcct ttgccgacgg caacgtcaaa tgcttcgtat ccgaacgcca ttcgggacgc
    13201 gttaccggcg atcgaaggca gcacaactgg ctgtttatcc gcgcaaaaaa ccacgccgcc
    13261 gccattatta ccaactggta cgaaggcagt tgcgactgga tggccatcgg caaagccgcc
    13321 tcgggaaacg ccgccagctc cccgataggc cccgaaatac ctgaaaccaa cgaagaaccg
    13381 caaagagaga gtggaagaac atcaaccgga ccccgaaacc gccgccgccg ccgagacggc
    13441 ttgctcgagg cactgcaaga ctagcgggct gtagagacgg gctgtagaga tggctgtaga
    13501 gatggctgta gagatggctg tagagatggc tgtagagatg gctgtagaga tggctgtaga
    13561 gatggctgta gagatggctg tagagatggc tgtagagatg ggctgtagag atgggttgta
    13621 gagatgggct gtagagatgg gctgtagaga tgggcttcag cccgccgatc caagcaatcc
    13681 gaccgaaacc ggccgcgccg ccaatccccc gaaacctatg ccccgccaat cctgccactc
    13741 ttcgtcattc ccgccgcttt cgtcattccc gcgaaagcgg gaatccagcc cctaaacgcg
    13801 gcaggaatct atcggaaaaa acagaaaccc ccgccaccgt cattcccgcg aaagcgggaa
    13861 tctagacccc aaacgcggca ggaatctatc ggaaaaaaca gaaacccccg ccgccgtcat
    13921 tcccgcgaaa gcgggaatcc agaccccgac gcaacaggaa tctatcggaa aaaacagaaa
    13981 cccccgccgc cgtcattccc gcgcaggcgg gaatccagac cccaaacgcg gcaggaatct
    14041 atcggaaacg gctgaaaccg aacggactgg attcccgcct tcgcgggaat gacggaaagt
    14101 ggcgggaata acgaaaggcg ggaatgaccg cgcaaaaagc cgctgccccc ttcggacggc
    14161 accggcaaca aaaaaccgca cggccgaaac cgcgcgggaa aggatagtcg ggcgcgcccg
    14221 ataagcagcg gccgcccccg ttatttcaat tgggcgacat attggcgcaa aacctcgttg
    14281 atgcgcgtct gccagccctt gccgccggcg cggaattttt cgaccacatc ggcggacagg
    14341 cgtatggtaa cgagttgttt cggggttttg cctgtgtttc gtttttgcat tacgcccttt
    14401 tcttccaatt gtttttgatg ggaaaaaagc acctgtgcca agtcttcggg cagggcttcg
    14461 gcaatggggc gggcaagcgc aaaatcttcg gcggcaagtt cccgcacttc gccgtcagcg
    14521 tttgttaagg attgacgttg catatttttt aacctctctt ttattcgctt tgcgaaaact
    14581 gatgacacgg atgccgtctt ttatcggcgt aaaacagaca atgtgcaggc gttgcgtatc
    14641 gcctagataa gcagcggcaa cataacgcgg ttcggggtaa tcaaagcaga catcgggcac
    14701 aataacggcc gttgtccagc gtatttgccc gactgattca aagggcaaat tccgctcttc
    14761 gatattgcgt tgattttttt cggagtcaaa ttcaatcttc attgcagctt gcagcgtatt
    14821 ttgtcgttac attataagcg gcaaaaaacc aaaatgtaaa tacaaaaaag gaaaccccga
    14881 aatgaccatc tatttcaaaa acggctttta cgacgacaca ttgggcggca tctccgaagg
    14941 cgcggttgcc gtccgcgccg aagaatacgc cgcccttttg gcaggacagg cgcagggcgg
    15001 gcagattgcc gcagattccg acggtcgccc cgttttaacc ccgccgcgcc cgtctgaata
    15061 ccacgaatgg gacggcaaga aatgggaaat cggcgaagcc gctgccgccg cccgtttcgc
    15121 cgaacaaaaa accgccacgg cattccgcct cgcggcaaag gcggacgaac tcaaaaacag
    15181 cctcttggcg ggctatcccc aagtggaaat cgacagcttt tacaggcagg aaaaagaagc
    15241 cctcgcgcgg caggcggaca acaacgcccc gaccccgatg ctggcgcaaa tcgccgccac
    15301 aaggggcgtg gaattggacg ttttgattga aaaagttatc gaaaaatccg cccgcctggc
    15361 tgttgccgcc ggcgcgatta tcggaaagcg tcagcagctc gaagacaaat tgaacaccat
    15421 cgaaaccgcg cccggattgg acgcgctgga aaaggaaatc gaagaatgga cgctaaacat
    15481 cggctgaaaa aatacgttta ccacctgttg gtagccatcg accaactgtt caacgcccta
    15541 accggcggcg cggcggacga aaccctctca agccgcacct atcgcggcgc gcggctcgcc
    15601 caaaagccca aaacccgctg aaagatttta tataccctga ttaacggcgt gtttttcgac
    15661 cgccaacact gtcggcaggc gtatatcagc gaactgaaag gcaggcagca cgacgcgcgg
    15721 ttcaaccaaa gccgcgccgc cggggaaaag gggacgcgat gaactacttc ggcatggtag
    15781 agtttctgcg cctgatggca atggtgcggc cgccatttgt cttcttctcc ggcacccgca
    15841 gcgaactgcc tgcttatctt gacctcgtgg cagaactgcg cctgaccgga tgggagcgtt
    15901 ttgcaggcag ccaaaccttg actgtgagca gcacatcaac agcaattcaa gctacgacga
    15961 ccacctgatt tataagttct gaccgcaagt agcgtactac ttttaaaggc ataagataat
    16021 ccccgtttaa cagaccatat aaacggggat aaatttgtgc aaaagctaat acaatttcct
    16081 acgcttcggc ggtgcaaaag ctgccgccaa ttcgtgcaaa agctgccgcc gccttacaat
    16141 ccgcccccaa atccccgaat ggctttaaaa catgcttgtc gcggagattt tgaaatcgga
    16201 caaactgtca ggcaatcttt ttcaatgttg acactacctc atcaaggtaa taacattgtt
    16261 attacataaa ctggtaaata
//
LOCUS       DAWFIE010000045        16140 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWFIE010000045 DAWFIE010000000
VERSION     DAWFIE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16140
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.045"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(86..2191)
                     /gene="fusA"
                     /locus_tag="WGQ71_001306"
     CDS             complement(86..2191)
                     /gene="fusA"
                     /locus_tag="WGQ71_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ0707313.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            complement(2210..2680)
                     /gene="rpsG"
                     /locus_tag="WGQ71_001307"
     CDS             complement(2210..2680)
                     /gene="rpsG"
                     /locus_tag="WGQ71_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ0707314.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            complement(2798..3169)
                     /gene="rpsL"
                     /locus_tag="WGQ71_001308"
     CDS             complement(2798..3169)
                     /gene="rpsL"
                     /locus_tag="WGQ71_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ0707315.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            complement(3427..7602)
                     /gene="rpoC"
                     /locus_tag="WGQ71_001309"
     CDS             complement(3427..7602)
                     /gene="rpoC"
                     /locus_tag="WGQ71_001309"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ0707316.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRAGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMLANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQE
                     VEQETAETQVTDE"
     gene            complement(7759..11937)
                     /gene="rpoB"
                     /locus_tag="WGQ71_001310"
     CDS             complement(7759..11937)
                     /gene="rpoB"
                     /locus_tag="WGQ71_001310"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ0707317.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNKLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(12127..12498)
                     /gene="rplL"
                     /locus_tag="WGQ71_001311"
     CDS             complement(12127..12498)
                     /gene="rplL"
                     /locus_tag="WGQ71_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ0707318.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            complement(12556..13056)
                     /gene="rplJ"
                     /locus_tag="WGQ71_001312"
     CDS             complement(12556..13056)
                     /gene="rplJ"
                     /locus_tag="WGQ71_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ0707319.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            complement(13286..13981)
                     /gene="rplA"
                     /locus_tag="WGQ71_001313"
     CDS             complement(13286..13981)
                     /gene="rplA"
                     /locus_tag="WGQ71_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ0707320.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKTTRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            complement(13981..14415)
                     /gene="rplK"
                     /locus_tag="WGQ71_001314"
     CDS             complement(13981..14415)
                     /gene="rplK"
                     /locus_tag="WGQ71_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ0707321.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQSMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            complement(14516..15052)
                     /gene="nusG"
                     /locus_tag="WGQ71_001315"
     CDS             complement(14516..15052)
                     /gene="nusG"
                     /locus_tag="WGQ71_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ0707322.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPMPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            complement(15054..15332)
                     /gene="secE"
                     /locus_tag="WGQ71_001316"
     CDS             complement(15054..15332)
                     /gene="secE"
                     /locus_tag="WGQ71_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ0707323.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            complement(15448..15523)
                     /locus_tag="WGQ71_001317"
     tRNA            complement(15448..15523)
                     /locus_tag="WGQ71_001317"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15488..15490),aa:Trp,seq:cca)
     gene            complement(15530..16078)
                     /locus_tag="WGQ71_001318"
     CDS             complement(15530..16078)
                     /locus_tag="WGQ71_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0707324.1"
                     /translation="MPIEDVFSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTC
                     TGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSK
                     EEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEG
                     LRFAIREGGRTVGAGVVSSVIA"
BASE COUNT         4059 a         4175 c         3605 g         4301 t
ORIGIN      
        1 gctaattcct ttacattaaa gatcgattaa agaacaatgc cgtctgaaaa atcatttcag
       61 cctttcagac gacctatttg cctgattagc ctttacgggc ttcagttaca gcagcagcta
      121 tgtgggcagg agcttcagaa tatttcttga actccataga gtaagtagcg cggccttggg
      181 ttgcagaacg caggtcggtc gaataaccga acatttctgc cagaggtact tcggcacgga
      241 cttttttacc gccgataccg tcatcatcca tacccaatac aacaccgcga cggcggttca
      301 agtcgcccat tacgtcgccc atgtattctt ccggagtttc cacttcaact gccataatcg
      361 gctcaagcag ggcaggagat gcttgacgca taccttcttt aaacgcttga gaagcggcca
      421 attcaaatgc caattgggaa gagtcgacat catggtaaga accgaatacc agacggatac
      481 gcacatcaac tacaggatag ccggcaacga taccgttagg caatgtatcg cggatacctt
      541 tatcgacaga cggaatgaat tcgcgaggaa tcacgccacc tttaatttca tcgataaact
      601 cgtaaccttc gccccccggt tccataggtt ccatttcaat cacaacgtga ccatattgac
      661 ctttaccacc ggattgtttc gcgtgtttgt attcagcttt aacggctttg cggatagttt
      721 cacggtaagc cacttgaggt gcaccgatat ttgcttccac accgaattcg cgtttcatac
      781 ggtcaacaat aatttccaag tgcagctcac ccataccgga aataatggtt tgaccggatt
      841 cttcgtctgt acggacacgg aaagaagggt cttctttagc caagcggttc agggcgatac
      901 ccattttctc ttggtcggct ttggttttcg gctcaacggc aatatggatt accggctcgg
      961 ggaattccat acgttccaag ataatcggcg cgctttccgc acacaaggtt tcaccggtag
     1021 taacgtcttt cagaccaata gcggctgcga tgtcgccggc gcgtacttct tcgatttcag
     1081 tacggtctgc ggcagtcatt tgtaccaaac gaccgatacg ttcgcgagtg ccttttacgg
     1141 agttcaatac ggtatcgccg gattttacta cgcctgagta aacgcggata aaggtcagct
     1201 gaccgacgta tttgtcgttc aacattttga acgccaatgc agagaatttc tcttcatcgc
     1261 tggcttgacg gctgtcggct tcctcggtat tcgggttgac accttgaacc ggaggaatat
     1321 cggtaggagc tggcagcaat tctacaactg cgtccaacat acgttgaaca cctttgtttt
     1381 taaatgcaga accacacagc ataggctgaa tttcgcctgc caaagtacgt tgacgcaacg
     1441 cgcctacgat ttcttcttcg gtcagctcgt cgccgcctaa gtatttgtcc atcagttctt
     1501 cgctggcttc ggctgcggct tcaatcatat tttgacgcca ttcttcggca gtttcgacca
     1561 attcggcagg aatatcgcca taggtaaagg ttgtaccttt atcggcttca ttccaaatga
     1621 tggatttcat tttcaacaaa tcaaccacac cgctgaagtt gtcttccgca ccaaccggaa
     1681 tgacgatagg tacagggttt gcgcgcaaac gggttttcat ttgctcgaca acgcggaaga
     1741 agttggcacc ctgacggtcc attttattga caaacgccaa gcgcggcact tggtatttgt
     1801 tggcttgccg ccatacggtt tcagattggg gttgaacacc gcccaccgcg cagtaaacca
     1861 ttaccgcgcc gtccaataca cgcatagaac gctctacctc tacggtaaag tcaacgtgtc
     1921 ccggggtgtc gatgatgttg aagcggtgct cggggaattg tttcgccata ccggaccagt
     1981 aggaagtaac ggcagcggag gtaatggtaa taccgcgctc ttgctcttgt tccatgtagt
     2041 cggtagtagc cgcaccgtca tgcacttcgc ccagcttgtg ggtcaaacca gtatagaaca
     2101 aaatacgttc tgtcgtcgtg gttttacccg catcgatatg ggcggaaata ccgatgttac
     2161 ggtacaggct gatcggggtc ttacgagcca ttttattagc ctttcaaaat tagaaacgga
     2221 agtgagagaa tgctttgttg gcttcagcca tacggtgtac ttcttcacgt tttttcaacg
     2281 caccgccacg accttcggac gcatcaatca attcgcctgc caaacgcagg tccatggatt
     2341 tctcaccacg tttgcgggcc gcatcgcgaa cccagcgcat tgccaaagcc aaacggcgtg
     2401 aaggacgaac ttcaacagga acttggtagt ttgcaccacc tacacggcgg cttttcactt
     2461 ccacgatagg cttggcgttt gcaatggctt cgttaaatac ttcgattgct actttgccgg
     2521 tttttttctc aatctgttcc aacgcaccgt aaacgatacg ctcggcaacg gattttttac
     2581 cgtcaatcat caatacgttc atgaatttgg tcaactcgac gctgccgaat ttaggatctg
     2641 gcagtacgtc gcgcttgggg acttctctac gtcttggcat tttaatttcc tttaatctat
     2701 tcagtcgggt ctattcccat gaatacccaa ttgagtattc acttactcgg ccgttgttca
     2761 gcttaggcgg ccgacgtgcc tatttaagtc ccagtaatta tttaggacgc ttagcaccgt
     2821 atttggaacg ggcttgttta cggtctttaa cgcctgcagt atccaaagaa ccgcgtacag
     2881 tgtggtaacg cacacctggc aagtctttta cacgaccgcc gcgaatcaat acgacactgt
     2941 gctcttgcag gttgtggcct tcgccgccga tgtatgaaat gacttcaaaa ccgttggtca
     3001 ggcggacttt acatacttta cgcaatgcag agttaggttt tttaggggta gtcgtgtata
     3061 cacgggtgca cacgccgcgt ttttgcgggc aggcttccag tgcgggcact ttgtttacgt
     3121 acacgggctt ttgacggcct ttgcgtacca attggttgat agttggcata ttttctcgtc
     3181 ctgttgagtt aaatacttgc cgacaccatg tcggcaagag tggaattata tttttattgc
     3241 aagcagacag tcaagcgtga gatcaggaag attcatcatt tttctttttt cagataaaca
     3301 tttaggtccc ttatgctcat aaacaactat taccggaaag gaaatcacta ataatttaaa
     3361 tgccgtctga aagaaaaacc acaagcctaa agcttgcggt tttgtattga ataaacgcac
     3421 caaagattat tcatccgtta cttgggtttc agcagtctcc tgctccacct cttgccattg
     3481 ttgatgacgg ctgcggtggt aagtcaaacc ggtaccggca ggaatcaagc gaccgacgat
     3541 gacgttttct ttcaaaccac gcaactcgtc ttgtttgccc ataatcgcgg cttcggtcag
     3601 gacgcgcgtg gtttcttgga acgatgcggc agaaatgaag ctgtcggtgg acagggaagc
     3661 tttggtaata cccagcaata cgttttcgta acgcgccggt tctttgcctt cttccaacgc
     3721 tttttcattg gcaagcatca catcgccgcg ttcgacctgc tctccggtaa tgaacccggt
     3781 ttctccggca tccgcaatgt tcacacggcg caacatttga cggatgatga cttcgatgtg
     3841 cttatcggaa atcttcacac cttgcaggcg gtaaacctct tgcacctctt ggacaatgta
     3901 gcgtgccaat gcttcgatac cttgcaaacg cagaatatcg tgcggatcga ccgcgccgtc
     3961 cacaatggtt tcgcctcggt ttaccacttg accgtcgtgt accagaattt gtttctcttt
     4021 ggaaatcaag gtctcgtatg ctacaccgtc cacgtcagta acaatcagac gttgcttgcc
     4081 tttggtctct ttgccgaagg aaacggtacc ggtaatttcc gccaacatac cggcatcttt
     4141 cggcacgcgt gcttcaaaca attcggcaac gcgcggcaga ccgccggtaa tgtcgcgggt
     4201 tttggaagag gcttgcggaa tacgcgccaa tacgtcgcct ttaccgattt cctgaccttc
     4261 gcgtacggta atcaccgcac caacggggaa tgccatggat accggagtag aagtaccggg
     4321 aatacagatt tccacgccgt tttcgtccaa gagtttcaca gtcggacgca gcagtttgga
     4381 agcactagag gaacgacgtt taccgtcaat caccaccaaa gtggacaaac cggttacatc
     4441 atcggtttgt ttggcaacgg taacgccctc ttccacgttt tcgaatttca ccatacctgc
     4501 gtgttcggta atcatcggac gggtatgcgg atcccaggtt gccaaggttt gaccggcttt
     4561 aatggccata ccgtcttgta ccagcaggat ggcaccgtaa ggtactttgt ggcgttcgcg
     4621 ttcacggccg atatcgtcgt gaatcacgac ttcacaagaa cggccgatga caaccaactc
     4681 gcctttattg ttggcaacat aacgcatttg gctgctgaat cgtgccgtac cgttggattt
     4741 ggcttccact tggctggctg ctgccgcacg ggatgccgca ccaccgatgt ggaacgtacg
     4801 catagtcagc tgggtacccg gttcgccgat tgattgggcg gcaatcacgc cgactgcctc
     4861 tccggcattg accagtttgc cgcgcgccaa gtcgcggccg taacagtgcg cgcacaaacc
     4921 gtggcgggtt ttacaagtaa tcggcgtacg gactttgact tcatcgacac cggattggtc
     4981 aatcatatcc accagttttt cagtcaacaa cgtgcctgct tcaaccaagg tctcgccgct
     5041 tgacggatcg acaacgtcag acgcagtaac acggcccaaa atacgatcgc gcaatgcttc
     5101 aatcacatca ccgccttgta ccactgcctt catgacaaag ccgtctgaag taccgcaatc
     5161 gtcttcaaca acgaccaaat cttgagttac gtctaccaga cgacgggtca ggtaaccgga
     5221 gttcgcggtt ttcaatgcgg tatccgccaa acccttacgc gcaccgtggg tcgcaataaa
     5281 gtattgcaat acggtcaggc cttcgcggaa gtttgaggta atcggcgttt caataatcga
     5341 gccgtcaggt ttagccatca acccgcgcat accggacaac tgtttaatct gagccgcaga
     5401 accacgggca ccggagtccg ccatcatata aatggagttg aatgactctt gatcgacttc
     5461 gttgccggca cggtcgataa ctttttgttt ggacaagtta tccatcatcg ctttagcgat
     5521 tttatcgccg gcacgacccc aaatatccac tactttgttg taacgttcgc cattggtaac
     5581 caaaccttga cggtattggt cttcgatttc tttaacctcg gcattggctt cagccagcaa
     5641 ggccgctttt tctttcggga tttccatatc gtcaacggca atggaaatac cgccttttgc
     5701 cgcaaatccg aaaccggtgt acatcaggtg gtcagcaaag ataaccgtat cgcgcaagcc
     5761 gcacaggcgg aacgatgcgt taatcagttt agaaatttct tttttcttca atgccttgtt
     5821 gacatattca aacggcaggc ctttcggcag gatttcgctc aacaatgcac ggccgacggt
     5881 tgtttcgtaa cggttaacga caggttcaaa ctcacctgct tcgtttttca cccattcgcg
     5941 cagacgtacg gtaattttcg tacccagctc gacctgtttg gtatggtatg cacgatgcac
     6001 ttctttcaca tcggcaaaca ggctgccttc gcctttggca ttgatacggt cgcgggtcat
     6061 gtagtacaaa cccaatacga tgtcttggga aggtacgata atcggttcgc cgttggctgg
     6121 agacaatacg ttgtttgaag ccagcatcag ggtgcgcgct tccatttgtg cttccaagct
     6181 caatggaacg tgtacagcca tttggtcacc gtcaaagtcg gcgttaaatg cggcacacac
     6241 caatgggtgt aactgaatcg ctttaccttc aatcaggata ggttcgaacg cttgaatacc
     6301 caaacggtgc agggttggcg cacggttcag cataatcgga tgttcgcgga tcacttcttc
     6361 caagatatcc catacttcag gtacttcttg ttctaccagt tttttcgcag ctttaacggt
     6421 agaagccaaa ccttgttttt ccaatttgtg gaaaatgaat ggtttgaaca actccaaagc
     6481 catttttttc ggcagaccgc attggtgcag acgcaggtat gggcctacgg taatcacgga
     6541 acggccggag tagtccacac gtttacccag caggttttga cggaagcgtc cgcctttacc
     6601 tttaatcatg tcagccaatg atttcagcgg gcgtttgttg gcgccggtca tggctttacc
     6661 gcgacggccg ttatccaaca gcgagtcaac tgcttcttgc aacatacgtt tttcgttgcg
     6721 gacgataatg tcgggcgcgt gcagttccag caggcgtttc agacggttgt tacggttgat
     6781 gacgcgacgg tacaaatcgt tcaaatcgga agtggcaaaa cgaccgccat ccaatggaac
     6841 caacggacgc aaatcaggcg gcaataccgg cagcacgtcc ataatcatcc attccagctt
     6901 cataccggaa cggtggaagg cttccaatac tttcaaacgt ttggcgattt ttttgatttt
     6961 ggtgtcggat ccggtcgatt ccaactcttg gcgcaggatt tcgatttcgc ccgctacatt
     7021 cagggtacgc agcaattcgc ggataccttc cgcacccatt ttggcatcga aatcgtcgcc
     7081 gtattcgtcc agcttgttgt agtaatcgtc ttcagtcagc aattggcggc gttgcagcgg
     7141 agtcataccg ggatcggtta ccacaaatgc ttcaaagtac aatacgcgct cgatgtcgcg
     7201 caaagtcatg tccagtacca tacccaagcg ggaaggcagg gattttaaga accaaatatg
     7261 tgcgacgggc gcagccaatt cgatatggcc catgcgttcg cggcgcactt tggacagggt
     7321 tacttccacg ccgcattttt cacacgttac gcctttaaat ttcaagcgtt tgtattttcc
     7381 gcacaagcat tcgtagtctt tgaccgggcc aaagattttg gcacagaaca aaccgtcacg
     7441 ctcaggtttg aacgtacggt agttgatggt ttcaggtttt ttgacttcgc cataagacca
     7501 tgagcggatg gtttcgggag aggcaatacc gattttaatg gcatcaaact cttcttccat
     7561 gccggcagtt tgcaacggat taaataagtt caacaaattc atttttgctc cttgaaggaa
     7621 gtatttttac cgcatggcgg attttcgata tggggaaacg gtacttttga tttcagacgg
     7681 cctaaatgcc gattgcttca ttcttattct gaaaccactt tttcagacga ccctgaaagg
     7741 ccgtctgaaa acttttgttt agtaacgttc caaatcgata tccaagccca gtgagcgaat
     7801 ctctttgacc aatacgttga aggactcggg cataccggca tcgattttgt gttcgccttt
     7861 gacgatgttt tcgtacattt tggtacggcc gttcacgtcg tcagacttca cagtcagcat
     7921 ctcttgcagc gtgtaggctg cgccgtatgc ttccagtgcc caaacctcca tctcaccgaa
     7981 acgttggccg ccgaactggg ctttaccgcc caaaggctgc tgggtaacca gactgtacgg
     8041 accggtagaa cgcgcgtgca ttttttcgtc aaccaagtgg tgcagtttca gatagtgcat
     8101 cacacctact gtaaccttgc ggtcaaatgc ttcgcctgaa cggccgtcat acagcgtgat
     8161 ttgggtttta ctgtcgttga agcccagttt ttcaacctca ggatcgtcgc tcggataagc
     8221 caagttcaac atttcgcgga tttcagactc tttcgcaccg tcgaatacag gagaggcgaa
     8281 agatgcacct ttacgcaggt tggaggccag ttcgatgatt tcttcatcag tcagggcatc
     8341 caaatcttct ttcttgccgc tgccgttgta gagtttgttc aagaactcgc gcaactcgcc
     8401 tgctttgcgt tgctctttca gcatacggtc gatacgctcg ccgatacctt ttgctgccca
     8461 acccaagtga acttccaaaa tctgaccgat gttcatacgg gaaggtacgc ccaatgggtt
     8521 cagtacgatg tctaccggac ggccgtccgc catgtaaggc atgtcttcca ctggcagaat
     8581 gcgcgatacc acgcctttgt taccgtggcg gcccgccatt ttgtcgccgg cttgcagacg
     8641 gcgtttgatg gcgataaaaa ctttcaccat tttttgtacg ccgggttgca attcgtcgcc
     8701 ttgggtcagt tttttcttct tgatttcgta taactcgtcc gcttcttcgc gtttttgttg
     8761 caggctcact ttaatcagtt ccaactgctt ggccaaatct tcatcggtca gacggatatc
     8821 gaaccaatcg tgcctgctcg gcagacccgc caaatattcg gtagtaattt cgctgccttt
     8881 agccagcttc atcggaccac cattggcttt ttgacccaca atcatacgtt cgatacggtc
     8941 gaatgcgtcg ttgtcgaaaa tacgcaattg gtcgttcaaa tccaaacggt agcgtttcaa
     9001 ctcggaatcg ataatggatt gggcgcgttt gtcgcgttga ataccttcac gagtgaagac
     9061 ttgaacgtcg ataacggtac cgctcatgcc ggtaggcata cgcaatgaag tatcttttac
     9121 gtcagatgct ttttcaccga agatggcgcg cagcagtttt tcttccggcg tcagttgggt
     9181 ttcgccttta ggcgttacct tgcctaccaa cacatcgccg gcttctactt ccgcaccgat
     9241 gtaaacgata ccggattcgt ccaaacggtt ttgcatacgc tcggacaagt tcggaatatc
     9301 gcgggtaatg tcttccgcac ccagcttagt atcgcgggca acgacattca attcctcaat
     9361 gtgaatcgaa gtatagcggt cgtccgcagc cactttttcg gaaatcagaa tcgagtcttc
     9421 gtagttgtaa ccgttccacg gcatgaaggc gatggtcatg ttttgaccca aagccaattc
     9481 gcccaaatcg gtggacgcgc cgtcggccac caaatcgccg cgttgcaaaa catcgccggc
     9541 tttgacggca ggacgctgat tgatattggt agactggttg gaacgggtga atttaaccaa
     9601 gttgtaaata tcgacaccca cttcaccggc agtcgcttcg tcgtcatgga cacggatcac
     9661 aacgcggttg gcatcgacat actcgaccac gccgcctcgg cgggcaacga ttgcagtagc
     9721 agagtcaacg gcaacggaac gctcgatacc ggtaccgacc atcggttttt caggacgcaa
     9781 gcaaggcact gcctgacgtt gcatgttggc acccatcaat gcgcggttcg cgtcatcatg
     9841 ttccaagaat ggaatcaggg atgctgcaac ggataccact tgaccagttg ccacgtccat
     9901 atattggacg cggtcgggcg ttgccataat ggtttcgcct ttttcacgac aggtaaccaa
     9961 atcgccaatc agattgccat ctgaatccaa atcggcattc gcctgtgcaa tcacatagcg
    10021 gccttcttcg atggcagaca agtaatcgat ttcctcggtt actttgccgt cgataacgcg
    10081 gcggtaaggc gtttccaaga aaccgtaatc attggtgcgc gcataaacgg acaatgagtt
    10141 gatcaaaccg atgttcggac cttcaggcgt ttcaatcgga catacgcgac cgtagtgggt
    10201 cggatgcacg tcccgcacct caaatcctgc acgttcgcgg gtcaaaccgc ccggacccaa
    10261 tgcggatacg cggcgtttat gggttacttc agacaaggga ttggtctgat ccataaactg
    10321 actcaattgg ctggagccga agaattcttt aatagcagca gaaacaggtt ttgcattaat
    10381 caaatcgtgc ggcatcaagt tttctgattc cgcctgattc aaacgttctt ttacggcacg
    10441 ttccacacgg gccaaaccgc tacggaattg gttttcagtc agctcgccta ccgaacgtac
    10501 tcggcggttg cccaagtgat cgatatcgtc cacttcgcca tggccgttac gcaactcgac
    10561 caaagtcgca atcgaggcga caatatcttc gacgctcagg acataaccgc ctttgtcggc
    10621 agcaccggca aacgtttcgt tcagcaggcg gccgtaccaa gagttttgtt gggcttcgga
    10681 cagtttttgt tcgtatgtgc gcgtattaaa tttcatacgg cctacgcggg acaaatcgta
    10741 gctgtcttca ctgaagaaca agcggttaaa caattgctcg accgcctctt cagtgggcgg
    10801 ttcgcccgga cgcatcatac ggtaaatcgc aacacgcgcc gcctgccggc cggcagtctc
    10861 atccgtacgc aaggtattgg agatataagc accctgatcc aactcattga tgtaaagggt
    10921 cgtaatttct tttacgccgt tgatatcaaa tttggccaac aactcttctg taatttcatc
    10981 attggcagaa gccaatacct cgccggtttc cgaatcaatc agatcggcag ccaatgcttt
    11041 gcccagcagg ctttctggtt ctacatccaa acgggtcagg cctgcattgg taatatcacg
    11101 gatatttttc gcagtaatgc gcttaccttt ggcaaccaat acattgcctt ctttatccaa
    11161 gatatcgacc ttggcagttt cgcctttcag acggcctgcg accaaatcgg tttgaacacc
    11221 gtttgaagac aaatagaacg tttctttgtc gtagaaaata tccaagattt gctcattgtt
    11281 gtagcccaaa gccttcaaca aaatcgttac cggcatttta cggcggcggt cgatacggaa
    11341 atacagcaaa tctttcggat caaattcaaa atccaaccat gaaccacggt agggaatgat
    11401 acgggcggag aataacaact tgccggaaga gtgcgtctta cctttgtcat gctcgaagaa
    11461 tacgccgggc gaacggtgca actgggagac aatcacacgc tctgtgccgt taatcacaaa
    11521 agaaccgctc ggggtcatca acggaatttc gcccatatac acttcgtttt cacgaacttc
    11581 ttttaccgtc ggtttagatg cttccttatc caaaatcacc aaacggatac gcgcgcgcaa
    11641 gggggctgca taagtgattc cgcgcaactg acattcggga atatcgaaca aaggctcgcc
    11701 caatgtgtaa tgcacaaact ccaatcgcgc ataaccgtta tggctcacaa tcgggaaaat
    11761 agaattaaat gccgcctgca gaccgtcatc ggtacgtttg tcaaaagcat tttccagctg
    11821 caaaaacttc gcataagaat caatttgggt tgctagcaag aaaggaactt ccaaaacatt
    11881 ttcccgcttt gcaaaactct tacggatacg ttttttctcg gtaaacgaat agttcataca
    11941 cactccgaaa agtaattttc aataataggc cgtctgaaac aacggtacgc atcaatctga
    12001 tatttacatt tatttgcaat gacttgataa aaactatgca aataaatgta aacaatactt
    12061 gatgtttatt ttaagaagca aaataaggct ggcagaaaac tgccagcctt cataagaagc
    12121 atcaaattat ttgatttcga ctttagcgcc tgcttcttcc agttgttttt ggatgtcttc
    12181 ggcttcagct ttagaaacac cctctttaat ggttttaggt gcgccgtcaa cgatgtcttt
    12241 agcttctttc agacccaaac cggtaattgc acggacaact ttaatcacgc cgactttttg
    12301 atcaccggca gaagccaaaa cgacatcaaa ttcggttttt tcttcagcat cggcagcacc
    12361 ggcaccagca ggacctgcaa ctgcaacagc agcagcagaa acaccgaatt tttcttcaaa
    12421 agctttaacc aagtcgttca attccattac ggtcaaagaa ccaactgctt ccaaaatgtc
    12481 ttctttagta atagccatgc tattttactc caaatattgt attaaaaaat aattgattaa
    12541 cagaaacaaa atcgattaag cggcttcttc gccggctttt ttctctgcca aagcagccaa
    12601 accgcgcgca aagcccgata caggagcttg cataacgaac aacagtttgg acaacagctc
    12661 ttcgcggctc ggaatagaag ccaactcagc aacctgagca gcattcatta cttcgccatt
    12721 gtaagaaccg gctttaacga caattttgtc atcttttttc gcgaattggt gcaacacttt
    12781 agcagcagca acagcatctt cagaagcagc gtaaaccaac ggaccaacca tttgatcggc
    12841 caattctgca aatgaagtac cttgcactgc acgacgagcc aaagtatttt tcagaacgcg
    12901 caaataaacg ccttctttac gtgcattcgc acgaagctca gtcatactgg aaacactgat
    12961 accgcgatat tcagcgacta cgagggtttg agcattagca attgccgcgc taatttcctc
    13021 gaccgccact ttcttggttt caatattgag actcaaggtc tacctcccac tgtttataaa
    13081 caggatacca aaacgatatc ccaccagcgc ggtaacctaa aaagacatct tacaagcaat
    13141 cttgcaatgt gtttcaggac taccgtctgc gtagggcagt tacgattaaa tcttacgatc
    13201 cctacggtct tggacatcta cttaatttta agtagcccaa attcaagcag cccagaaaac
    13261 caaactgctt gaacattcct taagattagt tatttacgct tgatgtatca acgcgaatac
    13321 ccaaacccat ggtgctagac acagcaactt ttttcagata ctgaccttta gcggcagcag
    13381 gcttggcttt aacgatagca tccagcaacg catcaaagtt ctctttcaaa tcagcttcag
    13441 cgaatgaagc acgaccaatc gttgcatgaa caatacctgc cttgtcggta cggtactgca
    13501 cttgacctgc ttttgcattt tttactgctt cggcaacatt aggggtaacc gtacctactt
    13561 tagggtttgg catcaagcct cgaggaccca aaatagtacc caactgacca acaatacgca
    13621 ttgcatcggg agaagcaata acaacatcaa agtttagatt gcctgctttg atttcagcag
    13681 ccaaatcttc gaaaccgacg atatctgcac cagcttcttt agcagcttct gcatttgcac
    13741 cttgagtaaa tacagccaca cgggttgtct taccggtacc tttaggcaga acgactgaac
    13801 cacggataac ttggtcagat ttacgcggat caacgcccaa gttgaaagat acgtcaacag
    13861 actcgtcaaa tttagcagtc gctgcttttt ttaccaaagc aattgcttca tcaattgcat
    13921 ataatttatt ggcttccaca gaagagcgaa gagctttcaa acgtttagat actttggcca
    13981 ttatacaaca ccctccacgt ccaagcccat tgagcgagca gaacctgcta tagtacgaac
    14041 agccgcatcc aagtcagcag cagtcaaatc aggctcttta gttttagcaa tttcttccaa
    14101 ctgagcacgg gtcaatttac ccactttatt ggtcagagga ttagaactac ctttttgcaa
    14161 accggcagct tttttcaaca agatagaagc tggcggggtt ttcatcacaa atgtgaacga
    14221 tttatctgca aatgcagtaa tcacaaccgg aatcggcaag ccaggctcca tactttgggt
    14281 tgcagcattg aatgccttac aaaattccat aatattcaaa ccgcgctgac ccaaagcggg
    14341 accaaccgga ggagatggat tggctttacc tgcaggaatt tgcagtttaa tatagccgat
    14401 aattttcttt gccacttaac ggactcctaa aaacgggtaa gacgcggaac catttccgct
    14461 ccccaagtta atttttgatt ttatcctctt attgcttttt tttcaagtat aaaaatcagt
    14521 taatcttttc aacctggctg aactccagct caacgggtgt ttctctacca aatatctgaa
    14581 cagacacgcg taacttattc cgttcataat tgacctcctc aaccacaccg ttgaaatccg
    14641 caaacggccc ttcatttaca cgaacctgtt gaccgacctc aaattcaact tttggtttcg
    14701 gcttctctat gccggtctga acctgctgta aaataatttc agcctctctc tgactaatcg
    14761 gcatgggtct attagccctc cctccaataa aaccggaaac acggggggtg ctttttacaa
    14821 gatgccaaga gtcatctgtc atttccatct caactagcac ataaccagga tatgactttc
    14881 tttcactaat agtcttacga ccattgcgga tatcaacaac tttctctaca ggcaccagaa
    14941 tttgtccgaa ataatctccc atctcctcac gggcaatgcg ctcttccaat attcgttgga
    15001 cattcttctc aaaccccgaa tacgcctgta caacatacca ttttttcgac atctcaacct
    15061 tcccttctca gcaatacatc aaaaaataac cacgaaattg ctgtatctgc cgcatagata
    15121 aatatagaaa gcacagcaac aaacactata acaaatacag tcattctgac agcatcttca
    15181 cgcttaggcc aaaccacctt tttgaattcg gaccaagaat ttgagaaata tgcaaaaaac
    15241 ccttccttac cggaattaga tgcagattct ttatcttgaa caaccagttg atccacttta
    15301 acgttctttt tttcaggcgt atgttctgtc atattattta tccatcatag catctgtcta
    15361 ttctcaatcc atgtaaatgg caagagagtt tactaaataa caaatacaaa aaaaattaac
    15421 cggcacaagg ccggttaatt ttttatttgg caggccaaga gggtctcgaa cccccaaccc
    15481 tcggttttgg agaccgatac tctaccaatt gagctattgg cctctaaact taagcgataa
    15541 cagaagaaac cacgccggca cccacggtac ggccgccttc gcgaatcgca aagcgcaggc
    15601 cttcttccat agcgataggc gcaatcagtt ctacggtaat ggttacgttc tcgcccggca
    15661 ttaccatttc cacaccttct tccaaagtaa ccgcgccggt tacgtcggtg gtacggaagt
    15721 agaattgggg acggtagttg gcgaagaacg gggtatggcg gccgccctct tctttgctca
    15781 gtacgtatac ttctgctttg aacttggtgt gaggagtgat tgtacccggt ttagccaata
    15841 cttgaccacg ctctacgtct tcacgtttgg taccgcgcag caatacgcct acgttgtcgc
    15901 ctgcttgacc ttcgtccagc agtttgcgga acatttcaac accggtacaa gtggtttttt
    15961 gagtttcttt cagaccgacg atttcgatct cgtcaccgac gtggatgata ccgcgctcta
    16021 cacgaccggt tactactgta ccacgaccag aaatagagaa tacgtcttcg ataggcaaca
    16081 agaaaggttt gtccacggca cgctcgggag tcgggatgta gctgtccaat gcagcagcca
//
LOCUS       DAWFIE010000046        15282 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWFIE010000046 DAWFIE010000000
VERSION     DAWFIE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15282
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.046"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(83..3173)
                     /locus_tag="WGQ71_001319"
                     /pseudo
     CDS             complement(83..3173)
                     /locus_tag="WGQ71_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            3447..4226
                     /locus_tag="WGQ71_001320"
     CDS             3447..4226
                     /locus_tag="WGQ71_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0707325.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFRFIPVTTRAANSDGLGGKRIPELLKNGSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(4426..5838)
                     /locus_tag="WGQ71_001321"
     CDS             complement(4426..5838)
                     /locus_tag="WGQ71_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0707326.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFTEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEEWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWYNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVA
                     DNPPTGLFANKKAARRALSSWAETYGLCPASAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGFWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
     gene            5929..6005
                     /locus_tag="WGQ71_001322"
     tRNA            5929..6005
                     /locus_tag="WGQ71_001322"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5963..5965,aa:Arg,seq:acg)
     gene            6023..6097
                     /locus_tag="WGQ71_001323"
     tRNA            6023..6097
                     /locus_tag="WGQ71_001323"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6056..6058,aa:Glu,seq:ttc)
     gene            6656..7831
                     /locus_tag="WGQ71_001324"
     CDS             6656..7831
                     /locus_tag="WGQ71_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ0707327.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(8042..8590)
                     /locus_tag="WGQ71_001325"
     CDS             complement(8042..8590)
                     /locus_tag="WGQ71_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ0707328.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLPPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(8593..10089)
                     /gene="ccoG"
                     /locus_tag="WGQ71_001326"
     CDS             complement(8593..10089)
                     /gene="ccoG"
                     /locus_tag="WGQ71_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ0707329.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            10618..12597
                     /gene="tkt"
                     /locus_tag="WGQ71_001327"
     CDS             10618..12597
                     /gene="tkt"
                     /locus_tag="WGQ71_001327"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ0707330.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQATVLPEGLPRVAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            12738..14126
                     /gene="fumC"
                     /locus_tag="WGQ71_001328"
     CDS             12738..14126
                     /gene="fumC"
                     /locus_tag="WGQ71_001328"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ0707331.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGKLNGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VWCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(14191..15114)
                     /locus_tag="WGQ71_001329"
     CDS             complement(14191..15114)
                     /locus_tag="WGQ71_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0707332.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
BASE COUNT         3843 a         4381 c         3524 g         3534 t
ORIGIN      
        1 gtcccgatag acctgcctcc ataaaaatgc cgtctgaaac ataccctgtt tcagacggca
       61 tccgcaaaac tacagttttc aattaaaact gccaatccag tttcatgctg acagtgcgcg
      121 gctctccata gaagttgttt gcgccgcgcg tacggttgta gttgttctca aaataagtgc
      181 gtccgtttaa gttcgtaccg atgaggctca atttggcgtg tttgcccaat tcgtaacgga
      241 cgaaaccgtc tatcagcccg tagccgccct gcctgatgtt atacagactg cttgtgccgc
      301 tttgtgcgga cacgccgccg ccgacggtca gccccgtatt cggtatatgg aagctcgttc
      361 cgaaacggaa tatgtgcacg ggtgtgaaat tgctgaagtt gtacgggtct gcgcctgtgt
      421 ttttggcgag gcgttcggcg ttgacttcgg cggcgttttt gtagcggctc ttgttgtagg
      481 tgtaacccgc aaagactttc caatcttcgt tcaactcacc cgacaactcg aattccgcac
      541 ccctgctgac cactttgcct atcggtttgg caacggtttg gaacgacccc tgcttgccgc
      601 ctgctccggg aacatagcca aaatcgacga ccgtgcggtt tttctgttcg aggtaaaaca
      661 atgcgaacga agcattcagc cgtccttgca agaacgcgcc tttccagcct acctcatagt
      721 ttgtgccgac caaaggcggt aaaacggttt tggcactgac atcgacatta tcctgctgtt
      781 tgaagatttt ggtataactt ccgtaaatac tctgttgcgg tgtcaaatca taggtaatgc
      841 ctgcataggg cgtcaattta tgaccttgca tcttggccgt ataatggtcc tgatccgcct
      901 taatgctcga tgccgtctga aaatcgcttg ccggctgccc atagcggaca ggcatatctt
      961 tggtttgcga ggtttcatag cgcgtgtagt gcagcccgcc caaaagatgc agtcggccgg
     1021 ttacgttgaa acgcgtgccg gcagtcagcg aatgggtttt gttggtgttg aggtatttgg
     1081 cgtagttata cagcgcagga acatgatcgt cggccacttt gacggttttc caaaccggca
     1141 ccgtaccgga aaaaccggta aaggcaggcg agccgtcggg attggtctcc tgaatcttgt
     1201 tgcctttttc gtccagctca tatacatcga catataccgg tgtccggctg ccgctgtatt
     1261 cgtcatagta atatacctgt ttgccttcgg catcgagctt gggctcggtt tttattttct
     1321 tggcgttcct gcattcttcg gcataaacgg tacggttgcc tttttcatcg tacgccttcc
     1381 aatcgggttc tttatgcccc ctgaccaaag gagacgacaa atcgccgtcc ggctcctcct
     1441 gacaacttcc cgcatacacg ccgtgcgttg cccccatatt cggacgtact ctgtggcggc
     1501 gttcgtagat ttccagatat tcggaacgta tcttttcatc gccgtaggca tagccgacaa
     1561 agaaatcgtg ttcccgcccg aacagcccat atgtgccggt caggtcaagt ttgattcccc
     1621 attggcggtc gtctttggta tgccgcaacg gcatataact gtatcgtcgg ttggcggtag
     1681 ccttacgatt aaaggaagag tcatacaggc tgtttggaaa acgttgcgcc gcattattaa
     1741 agatgccctc cttcgcaagg gctttatcga caaattccgc cttgtcggca tcaacgcccg
     1801 gatccccccc aagaaccttg acagataaag tccagcgcaa aaggggcact catacactcg
     1861 tcaaaaccgg ctttgcgttc tgcggcacgg cggctgcgat actgttcgaa agcagtatta
     1921 tcgaaacggt ttttaacaaa atcgtctttg cgctcccggt attccttggc ggtttcatca
     1981 cgatatgctt tcagtttctc caatgcctta tctttcggct cgaacgggat gacttcgttt
     2041 ttttcagtca aaaagcctac cgcatcctca cccgacaaac ccgccgcatg ttcgtttttc
     2101 agaaaaaact gccccacctt cgcatcggat tcattcttgg tataagacac ttcggcattg
     2161 agctgccaac cgttgtcaaa cacatgtttg aaacctgaga aaaggttgta tttgtcagca
     2221 ctcaaccgcg accaatcctc ccccaaataa gtgttgcgcg gcagttgcaa aggccggttg
     2281 caggcaggcg ttgaactgaa cggggcagtt ttctgatttt cacaggacaa aataatgccc
     2341 gaaaaatcag gaacctccct actcttctga tacatgccgc ccaaagtaag cacactgctg
     2401 tcgcccgcat cggcttcggc aatgccgtaa accatatgtt tcctgcccca aacgcggtct
     2461 ttaaacgatt ttttatactc ttccgcaccc accaaccttc cgcgtaaggt attcgcctta
     2521 ttcaggctgc ctgaaacatc caacactgca cgccggctgc cgcgatggtc ggcggtcagc
     2581 tctccggtat gtttgaaaga agcggtaggt cgcttacgga tcaaattgac ggttccaccc
     2641 ggctctgaat tggattgggt caaccccgtt gcaccccgta caacttcaat atggtcataa
     2701 accgccaaat cggtactcgg agacacgtcg attttcgccg tatatcccga acggcctgca
     2761 acattgacgg taataccgtc ttcaccaatc tgatcaatat agaaaccgcg tgacaaaaac
     2821 cgcgtctgca agcctgaatc gcgcacaacg ttgacacccg tcgtgttttt cattgcctct
     2881 tcaagcgtat gcaccgcctt atcgtcaagg cggctgcgcg tgatgacgct gaccgactgc
     2941 ggcgtatcct tgcccgcaat cctcatacct gtggcggtgg acatccgatc tatcgtataa
     3001 gaacgggtct tctcggtctt gcccgacaga gcatgagagc cgcgtacatt gaccgtatcc
     3061 agactgacgg tattgccgtc tgaaacaggc acaacaccgt ctgcaaaaga accaccgtaa
     3121 gccgataaca gcataacggt cagaatttta agtgaaaaat gattttgatt catagagacc
     3181 tctgtaatat gcaagtgtgc aaatcgtcca aaggccctca caactgtttt gattttttat
     3241 attaattgaa aaaaagtaat tctcaattaa atttatagat gggattgtat tcccattttg
     3301 acaaaaaaca aactgctcct taccgtttat ttcaaaaaac gataatattg tattgaaaaa
     3361 tatccgaatt taaatacaga tcgtcaatgc ggaaaaaaac acccaaattg gctataatcc
     3421 cgacaaacac actcaaggac aacaacatgg cagcctcgcc cgaagcaaaa ttcactgaag
     3481 aaaaaatttt gtgggtcaaa caccacacgc cgaaactcat cactttcgcc atcagccgtc
     3541 ccgaatccta ccgttttaaa gccggacagt tctcccgact cggtttctac gaaggagaag
     3601 gtttcatttg gcgtgcctac tccatcgttt ccgcagaata tgccgacacg ctcgaatatt
     3661 ttgccgtact catccaagac ggtcccatgt cggcccgttt cgccaaaatg caacagggcg
     3721 acaccatcct gctggataaa aatgccaccg gcttcctcct gcccgaacgc ttccccgacg
     3781 gcaaggattt ggtgatgctc tgcaccggct ccggcatcgc ccccttcctt tccattctcg
     3841 aacaacccga aatccggcag cgtttcgata ccgtcaacct gatacattcc gtatcttttc
     3901 ccgaagaatt gattttcaac gaccggctcg ccgccctttc cgaacaccct ctggtcggcg
     3961 aatacggaca ctcgttccgt ttcatccccg taaccacccg cgccgccaat tccgacggat
     4021 tgggcggcaa acgtattccc gaactcttaa aaaacggcag catcgaacag gcactgcata
     4081 ccaagttcac ccccgaatcc acacggttta tgatttgcgg caacccggaa atggtcaaag
     4141 acactttcca aacgctgctt gacatgggtt acgccatgca ccgcaaccgc attcccggtc
     4201 aaatcatgat ggaaaacggc ttctaaaaac caccctgctt gtccgatgcc tcggacgggc
     4261 aaaccggcac gacacgcaaa ccgcgtcggc aaaaatgccg tctgaagcct tcagacggca
     4321 ttttcggata catacaaacg gcaacacatt ccaacccaaa ccgattaggc aatcaatacg
     4381 aacggctgtt tacatactta ccggctttca ccaaccggta tcgatttaac cgatttcctt
     4441 aatatttttc ctgtccgttt taaacttcgc cttaaacgca tccggtaaat ctttatcgaa
     4501 ataccaaaac ccgtcatcca tttccaatgc gccgcccatt ccatgcagaa caactttttc
     4561 ccccgttaaa ggatcggttt cggtaatttc cacctcatgc atttttcccc aatccaaaac
     4621 aggcaacttg tgtgcaaacg ccaaaacctg ttcgtcagaa cggccgcacg ccttatcgca
     4681 tcggcctgaa acatatacgg gacaaggctc atcctctgca taaccatccg gaaggatacc
     4741 ggctgaggca ggacacaatc cgtatgtttc cgcccacgac gacaaagccc gtctcgccgc
     4801 ctttttattg gcaaataatc cggtaggcgg attatccgcc acaccgtttt tcaaagccgc
     4861 tgttttcgca ttcaacatgc cgtctgaacc tttttcaaac ctgacggtcg taaatgtttt
     4921 aagcagattt ttggcagata cataacaacc cgaatgataa cgcccgacca attccgcttt
     4981 aatcttatat gcatgcaggc tgcccaatgc aggaaaaaaa cggacttcct ccgtattgta
     5041 ccaatcaaac ggggctttcc cggaatccaa taaagccgaa acctcactat atacccgttc
     5101 aaacgtaccg atgtaattta ctttccctcc gccgtcgaaa caggccagca cccccatacc
     5161 gtcaggcaaa ccgtacaact gttccctcaa ccgttcgggc agcgcggcag gcagcggttt
     5221 cggattcatc aaacggaaac actgcctaat ccactcctca accccgtgtt ccgacagact
     5281 gtattccaaa taatcacaca atgccgacac atccgccatc gcacgatgcc tgtcttccac
     5341 aacaatcccc aacctttcga tgatactgtc caggctgtgc ttgtaaaatt gcggatacag
     5401 acaccgggac agttgcacac tgcacaaagc aggcgatgaa aatccgatac ccgcacgatg
     5461 aaactcatgc tttaaaaacg tatagtcgaa acggctgtta tgtgcaacca gcacacaacc
     5521 cttcaatacc gaaaacaact cgccggcaat ctctgtaaaa acaggtgcgt cggcaaccat
     5581 gccgtctgaa atccccgtca gccccgccac aaactgcgga atcggttttt gaggattaac
     5641 caaccactca tgcctcacca cccttccctg ctcaaacttg accaaagcca cttcggttac
     5701 cctgtcttca tacagattgc cgcccgtcga ttccaaatcg accacggcaa ccggcattcc
     5761 aaaccgtaaa aatacctttt ccagcaaggg ccagcgggaa gcaacaatca ttttattctc
     5821 tttaaattca aacaacaaac cgatatttta cacttttaag gcatttcatc caacaaaaca
     5881 attgacagaa tccgatgatt accctaaaat tcaaatcttt cttgcagcgc acccgtagct
     5941 cagttggata gagtatctgg ctacgaacca gagggtcggg cgttcgaatc gctccgggtg
     6001 cgccagtaag aaaatacaat atgcgcccat cgtctagcgg ttaggacatc gccctttcac
     6061 ggcggtaacc ggggttcgat tccccgtggg cgtgccaaat tctaaatccc cgagattatc
     6121 gctcggggat tttttattgt ctcagcaact cgttaccata tctttaccta cccccttcat
     6181 cagaatctca gacgtaatcg aatcatattc aaacctttgc cgtgcaaacc gatatcccat
     6241 aaccggatgc ggtgtccgtc caacatttta cccgattgaa acgcctgata tattgcaccc
     6301 catcaacgtg gcattacttt tcttaacaat cccctttgac agcaactgac tagggctttt
     6361 ttatgccatc atcaaattta tagtggatta actttaaacc agtacggcgt tgcctcgcct
     6421 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaattcac
     6481 tataatattt tctctcccga ttgaaacagg cgtaacagaa tatgcccgaa gctccggctg
     6541 ctttcttgtt taccgccgcg atatttagag tataatacca aatttgagca atagttctaa
     6601 aacagttaga accatttttc atgagcctga ctgattcgta cactcggaga aactgatgca
     6661 gaatattttt gaccctttgg ttattcgtgg aaaatccctt acccccatcg tgcaaggcgg
     6721 tatgggggtc ggtgtttccg catcgggttt atccagcgcg gtggcgcgtg aaaacggtat
     6781 cggaacgatt gccagtgtgg atttgcgcca ccttcacgaa gacctactcg ccgaatcaca
     6841 aatcaatccg agtgaagaga aatatacatc tttgaactgt accgcattag acagggaaat
     6901 ccaaaaagcc aaaagcgctt cagagggaaa aggactgatt gcggtcaacg tgatgaaggc
     6961 agtcaaagac cacgccgcgt atgtccgcca ggcttgcgaa tcaggggcgg atgcggtcgt
     7021 gatgggcgca ggcctgcctt tggacctgcc ggaaatgacc gagggctatc ataaagatgt
     7081 cgcgctgctg ccgattctgt ccgaatcgcg cggtattaat atcgtcttga aacgttggat
     7141 gaaaaaaggc atattgcccg atgcgattgt agtcgaacat cctgcccacg cggccgggca
     7201 tttgggcgcg tcaaccgtcg aaggcgtaaa cgatgctaaa ttcgacttca aacgcgtgat
     7261 tgaggaaacg tttgaagttt tcaaaagttt agggctggaa agcgaaaaaa tcccgcttat
     7321 tttggcaggc ggtatggcaa actttgaaaa agtcaaaacc gccctgaaaa actggggagc
     7381 atccgccgtt caaatcggta cggcttttgc cgttaccgaa gaaggagatg cgcaccttaa
     7441 cttcaaaaaa acgcttgctg gtgcggaaac tgaaaaagta gtcgaattta tgtctgttgc
     7501 cggtttgccg gcgcgcggcg tacgtaccaa attcctagac agctatatca ggcgcgaagg
     7561 caaacttcag gcaaacgcca aagccgaccc gcgccgctgc acccaaggat taaactgcct
     7621 gaccagctgc ggcctgcgcg acgggctttc caaagcaggg caattctgta ttgatatcca
     7681 gcttgctgcc gcattccgtg gagaagtgga taaaggcctg ttcttcagag gtaaagaccc
     7741 tcttcctttc ggcaatgcca tccgcaccgt ccgcgagacg atacaatatc tgctgacggg
     7801 gagcgaacct gttgcaacgc tcggacgctg acatcaggta ggatttgatt tgcaaacaaa
     7861 atgccgtctg aagggccttc agacggcgtt tttcaggctg cgttctggaa tagtgttaaa
     7921 aaataaacgg gatgagctgc atttattttc gtccgacaaa tcaaaccatc acgccgaatg
     7981 atcaaataat gcctgcacgg cgttgcatct ggcaaatcaa tgcaatgaaa acacggcttt
     8041 tttatttgct ttcagtatta ttgaaaagtt tgtccatcgg ggtcaaatcg accgcattgc
     8101 cttggctggt aatccatttg ttctcgacac gccacacgcc tgccgcgtcc tccactcgca
     8161 cataccagtg gttggtcgga ggaagggttt tgaacaccgc ctcatattcc gccctgccgt
     8221 tctgcgcgct gccgacgggc ttgagggcga cagtttggtc gtccgccttg cgggtcgggt
     8281 gcatcagcag caggttcaaa ggctgtttgc cgtcaaactc gccgccgaca aacacttttg
     8341 ccgcattcat atcgggggaa atgagaacct gcaccccgat atgccgtctg acggcttctt
     8401 catcccgatg aagctggatg tcgatatgct tgccgtcttt ataataatcg tccgtaacca
     8461 aatctgtcgc gtgctgctgc gcgacaaaaa acatagcgac gctggcgatg acgacaaaaa
     8521 tcggccccgc catcaagatc cacggccaga cgtgtttgta ccaaggtttg attggagtgt
     8581 tttgagacac ggttattctc cgataaaggt tgcatcttct tccaagacga ccggcttgcc
     8641 gtcgggcgcg ccgctttcgc ggtattggaa ggtaaattcg atagggtggc tgcctttgtc
     8701 cgcgtattcc ggaatggtgg acacttggac gggaagggtt accgtttcgc gcggggcaac
     8761 cttgataccg ccttcgggca gcccggtcag ggcgatttcg tcaaagcctt tgacacttgc
     8821 ggtaatgagc tgttcttttt cacttttgtt gatgatacgc aggctgtatg cgttttccag
     8881 ccagcctttg gcgttttcgc gcaccagtac gccacggtct ttcaaaatat cgacctcgac
     8941 cattttgcgc gtggacaaac cggccaggaa ggcaatgata actaacgcca acaccgcgcc
     9001 gtaacctgcc acgcgcggtc tgagcagccg ttttttaatg tctttttcag aatattcgtg
     9061 ttccaacgcg ctttcggtcg tataacggat taatccgcgc ggatagccca ttttgtccat
     9121 aatctcatca cacgcgtcga tacaggcggc gcagccgata cattggtact gcagaccgtt
     9181 gcggatgtcg atgccgacag ggcagacttg gacgcacatc gcacagttga tgcagtcgcc
     9241 caaacccgcc tcttccttat tgaccgtttt cttgcgcgcg ccgcgcggtt cgccgcgttc
     9301 cgcgtcataa gaaacaatca gcgtgtcctt gtcgaacatc gcgctttgga aacgtgcata
     9361 cggacacata tgcaggcata ctttttcgcg cataatgtgg gcgaagaaga aggtcataaa
     9421 gccgtaaaac gctgtggcaa acatcgcgcc gccgcctgct gctccagtga ataaatcggg
     9481 aacgaactgg cggataggga caaaccagcc tgcaaacgtg atgcccgtcc acgcgcagac
     9541 aaggaaaatc agcaggtatt tggtggcttt gatgcggatt ttagtgaaat tccacggcga
     9601 tttttccagt ttcagccgtt tgtttctatc gccttcgacc aggttgtcga tccacagcat
     9661 aatttcggtg taaaccgttt gcgggcagga atagccgcac cacagtcgcc cggcaatcgt
     9721 cgtccaccaa aacagcccga aggcgcaaat catcagcagc aaggcaaggt aaatcaaatc
     9781 gcccaccccc aacgacaatc cgaaaatgaa gaaatgccgt tcggggatat tgaaaacgac
     9841 ggcctgccta ccgctccagt tgaaccacgg aatgacgtaa aacacaaact gcgtcgccaa
     9901 tacggcggcg atacgcagtt tggcgaaccg tccttccgcc tttttgggat ggatgcgttc
     9961 gccttcggga tggatttgaa tcacgctggc tcgtggatcg aatgtttttt ttgctttcgg
    10021 cgcggctttt gtttgttcgg acgtgccgat tccggatgcc ggactgccgg cttggttttc
    10081 cgtggtcatt ctgcattcct tagatttttg attgatggtt tgctcgttac cgccgccgtt
    10141 tgcttttcag acgtcatttt tcttgttttt taaggcgttg tgtttcaagt tttgagaaaa
    10201 tccgtttttc ccaaaatata tttccgctat tgtacaactt tatgcgccgt ccggatgtat
    10261 ggggcggata catttcccat ccgcatcaaa acgcctggat tttaccttac cgcccgaaca
    10321 aaatccgaat acggttaaaa aaaaagacta aaaaaaaccg acacccccat atcggcagaa
    10381 ccgacggcgc aagctcataa acaaacgcta tcgacaatcc ggcacacaat ctataacttt
    10441 ttatttcaaa aggaataatg gcaggcttcg cccgcaaatc gaaaatcctt ccccaccggt
    10501 ctcctgccgc cgccttcccc cgcgtccgcc cttttcttga aagcataagc gaatcgggcg
    10561 ataatcaacg ctttccgatt atccacttat ccaaaacacc agcaaggaaa atacaaaatg
    10621 tctcaactgg caaacgcaat ccgcttcctc tcggccgatg ccgttcaaaa agccaattcc
    10681 ggccaccccg gcgcgcctat gggtatggcg gaaatggcgg aaacattgtg gacgaaattc
    10741 ctcaatcaca accccgccaa ccccaaattc tacaaccgcg accgcttcgt cctctccaac
    10801 ggccacgcgt ctatgctgtt gtacagcctg ctgcacctga ccggctacaa tctgagcatt
    10861 gaagacttga aaaacttccg ccaactgcac agcaaaaccc ccggccatcc cgaatacggc
    10921 tataccgacg gcgtggaaac cacgaccggc ccgttggggc aagggattgc caacgcggtg
    10981 ggtatggcat tggcagaaaa aatccttgcc gccgaattta ataaagacgg tttgaacatc
    11041 gtcgatcatt acacctacgt ctttatgggc gacggctgtc tgatggaagg cgtatcgcac
    11101 gaagcctgtt cgctcgccgg caccttgggc ttgggcaaac tgattgtttt gtatgatgac
    11161 aacaatattt ccattgatgg taaagtggac ggctggttta ccgaaaacat cccgcaacgc
    11221 tttgaaagct acggctggca cgtcgttccc aatgtaaacg gtcatgacac cgccgccatt
    11281 caagccgcca tcgaagccgc acgtgccgaa accggcaaac cgtccatcat ctgctgcaaa
    11341 accttaatcg gcaaaggcag tgccaacaaa gaaggcagcc acaaaaccca cggcgcacct
    11401 ttgggcgcgg acgaaatcga agccacgcgc aaacatttgg gctggactta ccccgccttt
    11461 gaaatcccgc aagaaattta cgatgcgtgg aatgccaaag aacaaggcgc gaaactggaa
    11521 gccgactgga acgaactgtt cgcgcaatat caagccaaat atcctgccga agccgcagaa
    11581 tttgtgcgcc gtatggataa aaaactgccg gaaaactttg acgagtacgt tcagacggca
    11641 ttgaaagaag tgtgcgccaa agccgaaacc atcgctaccc gcaaagccag ccaaaacagc
    11701 atcgaaatct tggcaaaaga gctgcccgaa ctggtgggcg gttcggccga cctgaccccg
    11761 tccaacctga ccgactggtc aaacagcgtc tccgttaccc gcgacaaagg cggcaactac
    11821 atccactacg gcgtgcgcga gttcggtatg ggtgcgatta tgaacggtct tgccctgcac
    11881 ggcggcgtaa aaccctttgg cgcgaccttc ctgatgttta gcgaatacga acgcaacgcc
    11941 ttgcgcatgg cggcactgat gaaaatcaac cctgtatttg tgttcaccca cgattccatc
    12001 ggtttgggcg aagacggccc gacccaccag ccgattgagc aaaccgccac cctgcgcctg
    12061 attccgaaca tggacgtatg gcgtccgtgc gacaccgccg aatctctggt ggcttgggca
    12121 gaagccgtca aagccgccga tcatccgtcc tgcctgattt tcagccgtca aaacctgaaa
    12181 ttccaagcgc gcagcgaaca acaactgaac gacatcaaac gcggcggcta catcatcagc
    12241 gaagcccaag gcaacgcgca agccgtcatc attgccaccg gttccgaagt cgagctggct
    12301 ttggaagcgc aaaaagccct cgccgcgcaa aacatcgccg tacgcgtcgt ttccatgccg
    12361 tccaccaacg tattcgaccg ccaagacgcc gcctatcaag ccaccgtcct gcccgaaggt
    12421 ctgccgcgcg ttgccgtaga agccggacac gccgacggct ggtacaaata cgtcggtctg
    12481 aacggcgcag tcgtcggcat caaccgcttc ggcgaatccg cccctgccga cttactcttc
    12541 aaagcattcg gctttaccgt ggacaatgtg gtcgatacag tgaaatcagt gctgtaaccc
    12601 cactcctaaa caaatgccgt ctgaacgctt ttcagacggc atttttttgc cctcccgcct
    12661 tgatgcttcc tcattccacc aatctccatt ataatatttg cgaatcactc ttattcacat
    12721 ttcaaaagga gaaacgtatg aacactcgca ccgaacacga caccatgggc aatgtcgaag
    12781 tcccgtccga agcctattgg ggcgcgcaga cccagcgcag ccgcaacaat ttcaaaatcg
    12841 gcggcgaaac cctgccgcag ccgctgattt atgctttggc gttggtgaaa aaagccgctg
    12901 ccgcgaccaa tgtttccctc ggcaggatta agccggaaca ggcggatttg attacgcagg
    12961 cggcggatga tgtgttgaac ggcaagctca acggacagtt tcctttagtg gtttggcaga
    13021 ccggctccgg cacgcagtcc aatatgaaca tgaacgaagt gctggcgaac cgcgccaacg
    13081 aaatcgccgg tacgggtttg gcggcgtatc agcccgtcca tcccaacgac catgtgaacc
    13141 acgcgcaatc gaccaacgac gcattcccga ccgctatcca cgttgccgcc gcgattgaaa
    13201 tcaaccgcca cctcatcccc gccgtaaaag ccctgcgcga caccttggac aaaaaagccc
    13261 aagccttcgc ccctatcgtc aaaatcggcc gcacccactt gcaggacgcg acgccgctga
    13321 ctttaggcca agaattttcc ggctacgttt cccagcttga tcacggctta ggccgtctga
    13381 acgatgcgct taaagacttg tatgaacttg ctttgggcgg tacggcggtc ggcacgggtt
    13441 tgaacagcca tcccgaatac gccgaaaaag ccgccgccaa actcgccgaa ctgtccggct
    13501 tgccgtttgt cagcgcgccg aacaaatttg aagccttagg cggacgcgat gccgccgttg
    13561 ccgcttcggg cgcattgaaa acgctggcgg caagcctgaa caaaatcgcc aacgacatcc
    13621 gctggctggc aagcggcccg cgttgcggtt tgggcgaaat caaaatcccc gaaaacgagc
    13681 cgggttcgtc catcatgccg ggcaaagtca acccgaccca atgcgaagca atgacgatgg
    13741 tgtggtgcca agtgttcggc aacgacgtta ccatcggtat ggcgggcgcg tcgggcaatt
    13801 tcgagctgaa cgtctatatg cccgttatcg cctacaacct cttgcaatcc atccgcctgt
    13861 tgggcgacgc gtgcaacagc ttcaacgaac actgcgccgt cggcatcgaa cccgtaccgg
    13921 aaaaaatcga ctatttcctg caccattccc tgatgctcgt taccgcgttg aaccgcaaaa
    13981 tcggttacga aaacgccgcc aaagtcgcca aaaccgccta caaaaacaac aaatcgttgc
    14041 gcgaaaccgc cgttgagttg ggcttgctga cgggcgaaga atttgacgaa ctggtcgttc
    14101 ctgccgatat ggttcatccg cgctaatctt tccctcaaat aaaatgccgt ctgaaacctc
    14161 gttcggacgg cattttccgt tgcctgcaaa ctagcggcgt ttgaacagcc tgtcccccac
    14221 cgccgctgta accgcacccc cgaccacgac cagtgcgccg gcataaccca aaccgttcat
    14281 atccggcgcg gcaaaagtat caggcatcac ataatgcccg agcaaagaaa atattacggt
    14341 aaacacgggg agcaaggttg ttaccgcgct gactttggaa gcctcccaat gtttcaacgc
    14401 ctcgccgaac gagccgtaac cgattaacgt attcaagcag caatacgcaa aacaaaccca
    14461 cgccaacgta ccgtccaaac ttccgatgtg tgccggttcg gcaaacggca ggaacacggc
    14521 ggcacttgcc gcataaatca acagcagaat ctgttgcggc ccgaattgcg ccgacagcag
    14581 cttttgcgcc acggcataac acacccatgc catactgcct gccgcacaca gcaacacgcc
    14641 cttcgcatac gcgcccaaac ccgacaactc gccgaattta tcgttaaaaa acataagcaa
    14701 accggcaagc agcaaaacca agccgatttt ctgagcggca gtcatccggt ctttaaacac
    14761 caacacaccg acaacaatca tcgtaaacgg cgaaatctgc cacaaaacct gcgtcgtggt
    14821 cggcgaaata taatgcagcc cttgggcaat caacacaaag tttgccgaaa tgcccgccac
    14881 gccgagcagc agcagcctga atgagcacca agaaaaatcc cgccgcttcg gcagccgccc
    14941 gcccaatgcc agcaaaacaa acaataccgc cgccgccacg gtaaaacgca cccacaccag
    15001 cgtcggcgca tcgacaaact tcaatacctg ccgcacggca atcggcagcg ttccccacgt
    15061 catcgccgcc aaaagtgcca acgcgaagcc taggagcggc ctttggtttt ccatcctgat
    15121 tttcctattt tttaaacaac cgtattgccg gacgatgccg gtttgccgca tcgggcaatg
    15181 atggttcaag cgtttggcgt ttgattccaa ccctttgatt tcaaacaaac cggctgaagc
    15241 ccggctattg cttcgcgcta tttgaaaaca ccgcctgaat tt
//
LOCUS       DAWFIE010000047        14931 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWFIE010000047 DAWFIE010000000
VERSION     DAWFIE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14931
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.047"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(78..332)
                     /locus_tag="WGQ71_001330"
     CDS             complement(78..332)
                     /locus_tag="WGQ71_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707333.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLSACLGMPLLLVSPAGVLDGA
                     AEALAIAAFLMLVARPPAVFGGLWKFNYSLADSHLKCNFP"
     gene            526..984
                     /locus_tag="WGQ71_001331"
                     /pseudo
     CDS             526..984
                     /locus_tag="WGQ71_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            884..1252
                     /locus_tag="WGQ71_001332"
     CDS             884..1252
                     /locus_tag="WGQ71_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ0707334.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            1321..2859
                     /gene="lnt"
                     /locus_tag="WGQ71_001333"
     CDS             1321..2859
                     /gene="lnt"
                     /locus_tag="WGQ71_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ0707335.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTTRSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQIGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3123..3986
                     /gene="rpoH"
                     /locus_tag="WGQ71_001334"
     CDS             3123..3986
                     /gene="rpoH"
                     /locus_tag="WGQ71_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ0707336.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            4470..5354
                     /locus_tag="WGQ71_001335"
     CDS             4470..5354
                     /locus_tag="WGQ71_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ0707337.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDAENGNQSLELNRDLVGQLAEINKDLR
                     KHYDLAKLGVADILRFPGVLASQRENADALAKVIADLTDSALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5438..5953)
                     /locus_tag="WGQ71_001336"
     CDS             complement(5438..5953)
                     /locus_tag="WGQ71_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0707338.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(6028..6447)
                     /locus_tag="WGQ71_001337"
     CDS             complement(6028..6447)
                     /locus_tag="WGQ71_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707339.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(6568..7566)
                     /locus_tag="WGQ71_001338"
     CDS             complement(6568..7566)
                     /locus_tag="WGQ71_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ0707340.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREAYTADVSFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLGLLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLQEEGEEPVLLLWAVAEDIRTLIRLAAALKQGQSVQSVRNSLR
                     LWGEKQTLAPIAVRRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            complement(7568..8047)
                     /locus_tag="WGQ71_001339"
     CDS             complement(7568..8047)
                     /locus_tag="WGQ71_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707341.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            complement(8099..8878)
                     /gene="pgeF"
                     /locus_tag="WGQ71_001340"
     CDS             complement(8099..8878)
                     /gene="pgeF"
                     /locus_tag="WGQ71_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ0707342.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGSHVGDNPDAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALDGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEI
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            9064..9255
                     /locus_tag="WGQ71_001341"
     CDS             9064..9255
                     /locus_tag="WGQ71_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707343.1"
                     /translation="MFQQGMAHQAPHCVLPERDYPPIRFYRHKQTGFNRKGMGMKSVS
                     DTSRAMPSENQSPLSDGIV"
     gene            9332..10306
                     /locus_tag="WGQ71_001342"
     CDS             9332..10306
                     /locus_tag="WGQ71_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ0707344.1"
                     /translation="MNILSKISSFIGKTFSLWVALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLTIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKTLNAFHHNAV"
     gene            complement(10433..11557)
                     /gene="rluD"
                     /locus_tag="WGQ71_001343"
     CDS             complement(10433..11557)
                     /gene="rluD"
                     /locus_tag="WGQ71_001343"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ0707345.1"
                     /translation="MQNTSFDNESDYSDDSDFVSASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMAVAKTLSAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAGDPVYG
                     NLRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPNDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            11556..12359
                     /locus_tag="WGQ71_001344"
     CDS             11556..12359
                     /locus_tag="WGQ71_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ0707346.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIATANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(12417..14636)
                     /locus_tag="WGQ71_001345"
     CDS             complement(12417..14636)
                     /locus_tag="WGQ71_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ0707347.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRPPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVADGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         3587 a         4491 c         3669 g         3184 t
ORIGIN      
        1 tgggtcagtt gcgtgtagct catggcaatc tttcttgcag gaaaggccgt atgctaccgc
       61 atactggcct ttttctgtta gggaaagttg cacttcaaat gcgaatccgc caggctgtaa
      121 ttgaatttcc acaaaccgcc gaacactgcc ggcggacgcg cgaccagcat caggaacgcc
      181 gcaatcgcca aggcttccgc cgccccgtcc aacacgccgg cgggagaaac cagcagcaaa
      241 ggcattccca aacatgcgga caaagtggtc gaaaccacgc tcaggaacaa cagtgcgcca
      301 cccggcagga acagactgcc catccaatcc acgctaacga ttctccctta aaattcatta
      361 aattaaaatc gaaagactgt aacacggcat ttttttcacg aaactttact tcacgtccgc
      421 caccgccttc agacggcatt ttcctgtttc atgccgcctg aaaccgccca aatccgcgtt
      481 ctcctatata atgtcggact ttgcgccgca tttcaggatt ctttaatggc atttgcctca
      541 ctatttaccc tgccggacga catcaccgcc gttttggacg atgttgccct gatgaccaaa
      601 gccgccgcaa agaaaaccgc cggcatggtc ggcgacgatt tggcacccaa tgccaaccgg
      661 gttacgggcg tatcggcaga acgcgagttg ccgattgttt ggtcgatggc gaaaggttct
      721 ttttccaaca agccggtttt ggtgcctgcg gccctgctgc tttccgcctt cctgccgcaa
      781 ctcattacgc cgcttttgac ggcaggcggc atttatctgt gctttgaagg cgtagaaaag
      841 ctgctgcaca agtttctgca ccgacatgac acgcatgctc gtaatggcgg caatcggcat
      901 cggcatgact gttttggtgt acggaatcgt cgccgtcatc gtcaaactcg acgacttagg
      961 catgctgctg atgcgccgcc cacaaacctt ttcgcgcagt ttggggcaaa tgctgactgc
     1021 ctttatgcct tgctttatgc gcggtttgag cgtggtcggc acgcttgcca tgtttcttat
     1081 cggcggcgta ctggtcgccc acaacctcgg ccttctgcac gatttcctgc acgcgcaaca
     1141 ctgggacgct ggctgggcgg agtacttcgc caacctcgtt gtcgggctgc tttccggttc
     1201 gattgcctgc gcccccgcct tgccgctgat gaatcgtttc ggcaggcatt gatttctttt
     1261 tcaaataccg atgccgtttg aaagatgttc agacggtatc ttccgaacag acagatgaat
     1321 atggtttcca aactggacaa atactggcag caccccgccc tctactggcc tttgctcatc
     1381 ctttttgccg ccgccacccc ctttaccttc gcaccctact accacttttg gctgatgccc
     1441 ttgattttcg gtgccttcgt ccgcctcatc gaactgcgtc cgcgttttgc tgtctcttcc
     1501 gcctacctgt tcggcctgac cgcatacacg acacagttct actggataca caccgccctg
     1561 cacgacgttt ccggcctgcc cgacctctat gccgtaccgc tgaccttcct actccccgcc
     1621 taccttgccc tttatccggc actgtgtttc tggctgtgga aaaaatttac cctgcctcgg
     1681 ggcataaaaa tcggtttggt actgcccatc ctgtggacgc tgaccgagtt tgcccgcgaa
     1741 cgtttcctga ccggattcgg ctggggcgca atcggctact cccaaatcac cccggacagc
     1801 ccgctcgccg gctttgcccc attgggcggc atccacatgg ttacactggc aaccgccttt
     1861 ctcggtgtct ggctggtttt ggcgagtgat aacaccacac gttcgggcaa acgcctgctt
     1921 ccaattatcc tgattgccgc cctgcttgcc gcaggctaca ccgcccgaca aaccgacttc
     1981 acccgccccg acggcagccg cagcaccgtc gccctgcttc aaggcaacat cgaccaaacc
     2041 ctcaaatggc gtgaagacca agtcatcccg accatacaga aatattacga acagatcggc
     2101 aaaaccactg ccgacatcgt catcctgccc gaaaccgcca tccccgtcat gcgccaaaac
     2161 ctgccggaaa acatactggc aaaatttgcc gaacaggcgc aaaacaacgg cagcgcgctc
     2221 gccgtcggca tcagccaata cacttcggac ggcaacggtt acgaaaacgc cgtcatcaac
     2281 ctgaccggtt atcaggaaaa caatcaggac ggcatcccct actacgccaa aaaccacctc
     2341 gtccccttcg gcgaatacaa accgctgccc ttcctgacca cgccgcttta caaaatgatg
     2401 aatatgcccc tttccgactt ccgcaaaggc ggcggtaagc aatccgccct gctgatgaaa
     2461 aaccaaaaaa tcgccttcaa catctgttac gaagacggat tcggcgacga actgattgcc
     2521 gccgccaaag atgccaccct gcttgccaat gccagcaata tggcgtggta cggaaaatcc
     2581 aacgccatgt accagcacct ccaacaatcg caggcgcggg ctatggaact cggacgctat
     2641 atggtccgcg ccaccaacac cggcgcaacc gccatcatct cccccaaagg caacatcatc
     2701 gcccaagccc aacccgatac ggaaaccgta ttggaaggac acatcaaagg ctatgtcggc
     2761 gaaacgccct atatgaaaac cggcagttca tggtggttga tgggcatatt gaccctagcc
     2821 gcactgattc ttttcatctt ccgaaacaaa gaacactgat acctgcacag cccgtccgga
     2881 cgggctgtct tttttcaaac cggctgttta catacaacta cgtatgtaat ataattccga
     2941 aatcggattg agccatcagg tttacaaaat caatcaaacc tgccttacaa aattttcctg
     3001 tctttcaaac cttagcgcat atctcctgcc aaacatacaa aatacaagcc aagcagtgat
     3061 atactgcatc ccaacacagc aaaccgccga tacgcagttt cagccgaaag gaaatgcccc
     3121 aaatgaataa cgctttcgca ttacccgcca tccaaagcgg caacggcagc ctcgaacaat
     3181 acatccacac cgtcaacagc atcccgatgc tgtcccagga agaagaaacc cgcctcgccg
     3241 aacgccggat aaagggcgac ctcaacgccg ccaaacaact catcctgtcc cacctgcgtg
     3301 tcgtcgtttc catcgcgcgc ggctatgacg gctacgggct gaatcaggca gacctgattc
     3361 aggaaggcaa tatcggactg atgaaggcgg tcaaacgcta cgagcccggt agaggtgcac
     3421 gcctgttttc atttgccgtg cattggatta aagcggaaat tcacgaattc atcctgagga
     3481 actggcgcct tgtccgcgtt gccaccacca aaccgcaacg caagctgttt ttcaatttac
     3541 gcagtatgcg taaaaacctc aacgccctgt ctcccaaaga ggcacaagac atcgccgacg
     3601 atttgggtgt caaactttcc gaagttctgg aaatggaaca gcgcatgacg ggacacgaca
     3661 tcgccatcat ggcagacaac agcgacgacg aggacagttt cgcccccatc gactggcttg
     3721 ccgaccacga ttccgaaccg agccgacaac tatcgaaaca ggcgcattac gccctgcaaa
     3781 cagaaggttt acaaaacgct ttggcgcaac tggatgacag gagccgccgt attgtggaaa
     3841 gccgctggct tcaagacgac ggcgggctga cgctgcacca gcttgctgca gaatacggcg
     3901 tctctgccga gcgcatccgc cagattgaag caaaagccat gcagaaactg cgtggtttcc
     3961 tgaccgaaga agccgaagcg gtttgaaata tacgcaataa aatacccgac aaattgtttt
     4021 gtcgggtatt ttatttggta aacatcaatg cggtttgtgc gtcgtgctgt acacgatttt
     4081 atccgtccac gccgttgcaa ttgccgaaat caaaaagcag acatgcacca gacactgccg
     4141 catcagctgc ttttccgaca gattggcgga attgatatag tggattaaca aaaatcagga
     4201 caaggcggcg aagccgcaga cagtacaaat cgtacggaac cgattcactt ggtgcttcag
     4261 caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg gtcttggtta
     4321 atccactata catttccctt ccttgcagca acaaaagcgc gtgattccaa tctgccgctg
     4381 caaaatcttc aatttttcac acccgaaaac gcttgccgcc atacagatgt gatatattga
     4441 ttccatttta acgggcttcg gagaacgaca tgagcagcgg aaatatccac atccacagca
     4501 tgaccggatt tgccaacgca gcggcagagt gcggcagcaa acgcatcaat ctcgacctgc
     4561 gtgcggtcaa ccaccgtttt ctagatattc aaatcaggat gcccgacgat ttgcgctatt
     4621 tggaaagcgg tatccgcgag aaaatttcat cccatatcgc acgcggcaaa gtcgaatgca
     4681 aaatccaaat tcaagatgcg gaaaacggca atcagtcttt ggaactgaat cgcgatctgg
     4741 tcggacagct tgccgaaatc aacaaagacc tccgcaagca ttatgatttg gcaaaactgg
     4801 gcgttgccga tattttgcgc tttccgggcg tattggcaag tcaaagggaa aatgcggacg
     4861 cattggcaaa ggttattgcc gatttgaccg attcggcact gaaggacttt accgccgcac
     4921 gccggcgcga agggaaaaaa ttgggcgagc acctgttaca acgcctcgaa agcatggaag
     4981 aaatcataga tgcattaagc gaactctttc ctaccctgtt ggaaacacat aaagaaaaaa
     5041 tccgcgcccg ccttgccgaa gcggtcggca gcatcgacaa cgaccgcctg caacaggaat
     5101 ttgccctctt tatccaaaaa tccgacatcg acgaagagtt cagccgccta cgcacccaca
     5161 ttgccgaagt gcgccgtatc gttaccgaac acaaaggcag cagcggcaaa cggctggact
     5221 tcctgatgca ggaattaaac cgcgaagcca acactttggg cagcaaatcc atcgctaccg
     5281 aatgcaccca agcctcggtc gagctgaaag tcctgatcga acagatgcgc gaacaggtgc
     5341 agaatatcga ataatcggtt tccccgataa gcaaatgccg tctgaaagcc ctttcagacg
     5401 gcattttttc agtttgaaac ctttgcggta taaaggctta aggctttcct ttgaccgcca
     5461 ccaatcttgt tcctgcaaga aaatcataca gaaactgccg atcgggattc agcagggcga
     5521 acccccacgg taaaatcagc caaatcaatg ccgcgccggc cgcgcccttg ggcggaatgc
     5581 cgaggaagtg gcgcaatccg gcataagcaa gcataggaat gaagacgatg aatatgcacg
     5641 cccaaataaa gcgcaggcgg agtaagtgta gagacggctg tatgccgtta aggtcgcaca
     5701 aaccgatttt ccacgtcctc atcgccaagg tctgtccctg cccgtgccag ttggcacgga
     5761 aataaagcca ccatgatccc attatcagga tactcgttac caatgcagaa accgcaatag
     5821 aaacgggatt cagaaaaatg gcggcaatac cggcaaacaa tgctgccaaa caggttgccg
     5881 caccgaccag cagcatttcg tacagcatgg cggcaaagcg gcgtttgagc ggggcggttt
     5941 tcagtatcat catcttttca atcggaatat gccgtctgaa acgccaacag gcttcagacg
     6001 gcattcatgc agatatgtct catgtggtta acggtgcgga cgtttctgac cgcgcgactg
     6061 ttttgccgcc atttgattga tttcctgacg tgtcgggcgt tttttgaaca tagcgtgcgc
     6121 ctcgtccacc gaacattttt gcgacaggca gatcagggta atcgcatctt tctggtgttg
     6181 tccgccgctg tggtacatac ggcgcaggtt tgcctcgttg attctgcctt tttcgggatc
     6241 gatgtcgaat tttttcaggc gttcgataaa ctggcgttgc tgtcggcggg atgtccaaac
     6301 aaagccgccc gcagcggcgg cgacgatgac ggcggcaagc aggatggcaa gcggccagga
     6361 atcgacaatc aatacgataa tgaggcttaa aacggcggtg gtcagtaaaa tcaggccgcc
     6421 caagcgcagt ttggtcttat tgtccatagg gttaatgttc ctttattgag gtggtcgtgc
     6481 cgtctgaaat gttttcggac ggattggtga atacttttca ggattaacgg tattttacag
     6541 tattttcgga ttttggggat taccgcttta ttccgccagc gacacgacaa gccgtttgaa
     6601 taccgtccat gcgtcgccgt cttccgcacc tttgatgatt cggtcgattt gggcgcaggt
     6661 tttgagcgcg tcaagcaggc ggacgacgga aatccgtctg accgcaatcg gagcgagcgt
     6721 ctgcttttca ccccaaagtc tgagactgtt gcgtacggat tggacactct gcccctgttt
     6781 caaggcggcg gccagacgga tcagcgtcct gatgtcttcg gctaccgccc acagcaacag
     6841 caccggttct tcgccttcct cctgcaatcc gtccaacaac ctgcatacgc gcaggacatc
     6901 gcccttcatc cacgcgcctg ccagttggaa cgcgtcgaag cgggcgacgt tggcaacggc
     6961 ggtttgcgcc tcatcgatat tgacggtatg ccctttcgga tacagcagcc cgagcttgtc
     7021 gatttcctga cgcgccgcca acagattgcc ttccacgcgc tcagcaaaca gtgccaatgc
     7081 gtctgcctcg atacccaaac cgattttgtc cagccgtccg cgtatccatt ggggcaaagc
     7141 cgccgcgccg accggtttgg cttcccacac ttcccccttt gccgccaatg cggcaaacca
     7201 tttggactgg agctgggttt tctccagttt gggcagtaaa accagcgtta ccgtatcttc
     7261 cggcaggcgg gcggcaaaat cctgcaacgc ctcgccgccg gttttgccgg gcttgccgtt
     7321 agggatatgc agttccaaca gcttcaaatc ggcaaacaga cccgcactgc ctgcggtttg
     7381 cagcagttcg ttccaatcga aagacacgtc ggcggtatag gcttcgcggt tgagataacc
     7441 ctgcttcttc gccgccgccc tcaatgcgtc caatgcctcg atacgcaaca gttcttcctc
     7501 gccgtggatg acgtacagag gtttcaaagg cgcgtccgta tcaatgcgtc cgatatgtgc
     7561 cgccacgtca ttccgccttc agaaaggtca ggcggcggac aatctgttcg gcggcatcct
     7621 gccgcatttc cgcccacaat gccgcttcct cttcctgttt gcccaagatc tcgttgtcgg
     7681 cataagcaag gacgcggcgg acggacacgg tcatcggttt gccgaccggc tcgccgcgtt
     7741 tcaatacctg cgcttcaacc gtcaagatta aaagatattc gttgatgact gccgcacggg
     7801 taacggtgta ggtttccttg ttttgggaaa cgctgtctat acgcagggtc atctgcgcgc
     7861 cggcagcatc gtccaccctg cccgaagcct gatacagcgc ggtttccaaa ggaaactgca
     7921 atgcctgtcc gccttcgatg tgccagctcc ggtaggtcag cggcggagaa atgccgtctg
     7981 cacctttcag gtggaaaccg cacgcgccca gcatcagcac tgcagcagta agaaacagtt
     8041 tgttcatggc ttgcctttct caaaagcaca aagtcggtag attatatcag cggcgtgctc
     8101 agacggcatt gccgtccagc caaatcaggc tcgccatacg ccctgtcgct ccgtcgcggc
     8161 ggtaggaaaa gaaagtatcc cgttccaaaa ccgtacaatg cgtgccgccg tatacgccgc
     8221 ctacgccttc gcgcttcaga atcaggcggg cgagcgcgta aaggtcggca aggaatttgc
     8281 cgccgcctat gccttcaaat gcggcggcgg cttcgggcat gggcgtgcag aacgcatcaa
     8341 acacgtcctg cccgacttca aacgcatccg cactgatggc agggccgaga tacgccataa
     8401 tttcgacagg cggaaccttc atcgcggcaa tggtgttttg caatacgccg cccgccaaac
     8461 cgcgccagcc cgcgtgtgcg gcggcaaccg ccgtacctgc cctgtcgcaa aatagaacgg
     8521 gcagacagtc ggcagtcatg acagcacagg caaccttgcc cgtgtcgtcc acggaagcgt
     8581 cggcatcggg cgtgccgtcc aatgcttcgg cagcgttgac gacgacggtg ctgtggattt
     8641 gattgaggta ggcaacgggc aatccgacct gctgttgcac gatttcgcga ttgcggcgta
     8701 cggcatcggg attgtcgccg acgtgcgaac ctaagttcaa actttgatac gcgccctggc
     8761 tcacgccgcc gttgcgcgtg gtaatcaggg ttttcacatt ggcgggcgcg ggccaatcgg
     8821 cggtcaggaa gtttttgcct ttcggggcaa gatttagggt ttctgtgatg gttttcattg
     8881 gatattggaa taggtgtgtg gcggaaaggg ttaattaact gccttcgggg caaatggatg
     8941 tttgtttttt cgtcattccc gcacaggcgg gaattcggag attcgggatt gttttcaaac
     9001 gttcgggttc ggattcttgc cggtgcggga atgatgtcct tatatacttt ctccaagctt
     9061 tatatgtttc aacaggggat ggcacatcaa gcaccgcact gcgtgttgcc cgaacgagac
     9121 taccctccga ttagattcta tcgccataaa cagacgggtt tcaaccgaaa aggaatggga
     9181 atgaagtccg tttccgacac ctctcgggcg atgccgtctg aaaaccaatc tccactttca
     9241 gacggcattg tttaggcgca gtcattttgc aaattaaggt taaggcgtgt atgcttgctt
     9301 tcccttaaaa acaaacacgc aacgcattat tatgaatatc ctcagtaaaa tcagcagctt
     9361 tatcggaaaa acattttccc tctgggtcgc gctctttgcc gccgccgctt ttttcgcgcc
     9421 cgacaccttc aaatgggcgg ggccttatat tccttggctg ttgggcatta ttatgttcgg
     9481 tatgggtttg acgctcaaac cttccgactt cgatattttg ttcaaacatc ccaaagccgt
     9541 catcatcggc gtaatcgcac aattcgccat tatgccggca accgcctggc tgctgtccaa
     9601 actgttgaac ctgcctgccg aaatcgcggt cggcgtgatt ttggtcggct gctgcccggg
     9661 cggtacggct tccaatgtga tgacctatct ggcacgcggc aatgtggctt tgtcggttgc
     9721 cgttacgtct gtttccaccc tgatttcccc attgctgact cccgccatct tcctgatgct
     9781 tgccggcgaa atgctggaaa tccaagcggc cggtatgttg atgtccatcg tcaaaatggt
     9841 tttgctcccc attgttttgg gtttgattgt ccataaggtt ttgggtagca aaaccgaaaa
     9901 gctgaccgat gcgctgccgc tggtttccgt tgccgccatc gtgctgatta tcggcgcggt
     9961 tgttggggca agcaaaggca agattatgga aagcggcctg ctgatttttg cggttgtcgt
    10021 actccacaac ggcatcggct acctgctcgg cttctttgcc gccaaatgga ccggcctgcc
    10081 ttatgatgca caaaaaacgc tgaccatcga agtcggtatg caaaactcgg gcctggccgc
    10141 cgcgcttgcc gccgcacact ttgccgccgc gccggtcgtt gccgttccgg gcgcattgtt
    10201 cagcgtgtgg cacaatatct ccggctcgct gctggcaact tattgggcag ccaaagccgg
    10261 taaacataaa aaaaccctaa acgcctttca tcataatgcc gtctgaacgg ggcaaacaaa
    10321 accggcggtt tctgcccggc cccttcagac ggcctttgtt tgaaatcggc ggggacgatt
    10381 gtccggctgc tccgattcgg ctgcctgcgt tcgccccgcc tttcaagccg cctcactccc
    10441 ttacataaac cacttcgaca tcatagtcgt cttcgttcca atcatcgtcg tcgtccgcgc
    10501 cgaatttgtc ctgccattcc tcttcattgc tcaaagacga atccaaaccg gcttcaagac
    10561 ggaggacgga caacaaatga tatatgtcgt ttggaatcgg tgcttcaaac gaaacggttt
    10621 cgccgctttc cggatgagtg aaacttaagc ggtaggcgtg caatgcctga cgcgcgccca
    10681 gacttttaac ggcttctttc accgtgtcgc cgcacgggtg gcgcaggttg ccgtaaaccg
    10741 ggtcgccggc aagcggatgg ttagcttcgc gcatatgtac gcggatttgg tgcgtcctgc
    10801 ctgtttcgag cgaacactcg atatagctgt gggcaagata gcgttccaac actttgacgt
    10861 gggtaacggc tggtttgccg ccgaatttga cgactgccat tttcaggcgg ttgtgcggat
    10921 cgcgcccgat ttgggtttca attttaccat caaagggaac gatgccattg gcgacggcgc
    10981 ggtagatgcg tttgactgtg cgttcctgaa gctgccgcac aagggaattt tgcgcggaca
    11041 gggttttggc gaccgccatc agcccgctgg tttccttgtc caaacggtgt acgatgcccg
    11101 cgcgcggaat ttgggacagc tcggggcaat gcgccaacag gccgttgagc agcgtccccg
    11161 tccagttgcc cgccgccgga tgcaccacca gtccggccgg tttgttgacg acgatgacgg
    11221 tatcgtcttc gtaaacaata tccaaagcca taggctctgg aacaaacgcc agattttcct
    11281 cactcggacg gacggttaca caaatttgct cgccgcctat cattttgtct ttgggttgcg
    11341 aaggtttatc gtttacaata accgcgcctt ctttaatcca tgatgtcagg cggctgcgcg
    11401 agtagtcggg cagaagtttc gccaataccg catccaaccg cccgccggca agctcgagcg
    11461 gaacggtcag accgatacgg ttttcagttt cggaagctga cacaaagtct gaatcatcgc
    11521 tataatcgga ttcattatca aaggaagtat tctgcatgaa aaaaattctt ttaacggttt
    11581 cattaggttt ggcgctgagt gcctgtgcca ctcaaggtac ggtagataaa gatgcccaga
    11641 ttacccaaga ctggagtgtg gaaaaactct atgccgaagc ccaggatgag ctgaacagca
    11701 gcaattatac tcgagctgtc aaattatacg aaatcttgga atcgcgcttc cccaccagcc
    11761 gccatgcccg gcagtcgcaa ctggataccg catacgccta ttataaagac gatgaaaaag
    11821 acaaagctct ggcggcaatc gaacgcttcc gccgcctcca tccgcagcat ccgaatatgg
    11881 attacgcgct gtatctgcgc ggcttggtgc tgttcaacga agaccagtcc ttcttgaaca
    11941 aactggcctc gcaagactgg tccgaccgcg acccgaaagc caaccgcgaa gcgtaccagg
    12001 cgtttgcgga actcgtccaa cgtttcccca acagcaaata cgccgccgat gcgaccgcac
    12061 gcatggtcaa actggtcgat gcactgggcg gcaatgaaat gtcggtggcg cgctactaca
    12121 tgaaacgcgg tgcatacatc gccaccgcca accgcgccca aaaaattatc ggcagctatc
    12181 aaaatacgcg ttatgttgaa gaatcgctcg ccatattgga acttgcctac caaaaactcg
    12241 gcaaaccaca gcttgccgcc gatacgcgcc gcgtgttgga aaccaacttc ccgaaaagcc
    12301 cgtttttgac gcacgcttgg cagcccgacg atatgccctg gtggcgttac tggcattaag
    12361 cccatcttcg taatgccgtc tgaagccgtt tcagacggca ttttatgttt caaacctcat
    12421 tcaaacggtt ttttctgcca atagaattta taccctctca aacgttgagc cttcacgccg
    12481 ccgcgtccca agccgaattg cagtgcgccc gacaaatcgg tacgcagcag tttgatgccg
    12541 tgtgcgcgga cacggttctg caccgcttcg gtcggatgtt tgtaggcgtt ggcataaccg
    12601 cttgaagcaa cggcatattc gggcgagacg gcattgagga acacgcccga cgaggacgta
    12661 ttgctgccgt gatgtcccaa aaccaaaacc tggctgtaca ggttgcctcc atacttgccg
    12721 accaggcttt cctcgccctt cgtatccaaa tcgcccgtta ccagcagtgc cgcaccatca
    12781 gccacaacac gcaaaacaca acttttccca ttatcatcga tgtttttgcg ttcagacggc
    12841 atcaaaaact cgaaatccac gccgtcccat tgccaacgct gttccgcaca atgccgcgcc
    12901 ccctcataaa attccggctg cccggcataa atcccgccgt tcggtatctt gccgactgcc
    12961 tgaaaaccgc cgtcgtggtc gctgtcgtga tgcgacaaaa ccagcttgtc caaacggcgg
    13021 acacccgccg cattcaaact cggcacaatc cccatctgcg ccgccgctgc cgttccggtg
    13081 tcaaacaaaa gatgatgatt tgccgtctga accgacaccg acaaaccctg ccccgcatcc
    13141 caaaccgtaa ccgcaacctc attttccggc acgccgggcg gacggtaaaa cacaaaccct
    13201 gccaacagca acaccgccca cggacgcaaa cccaagccgc gcggcagcag caacagcaaa
    13261 gcggcacaca ccgccaacac caacagcggc aaaggtgcgg aggcaacggc aaactcgggc
    13321 gacacatcgg caagccacac caaaaaccgc aaagtatatt ccgccaaaaa tgcccccaac
    13381 tgttgcaaag gcgcaaacgg cacgaccgaa cccagcaacg ccagcggcgt caatacccaa
    13441 gaaaaccacg gaatcgccac cgcattgacc aaagggctga ttaaaggcag cgaagcaaac
    13501 agataaccga gcaaaaccag cgacaacacc gaagccgccc actgcccgcg caaagcagtt
    13561 tgccgttttc cctcgtgcaa acgccccgag cacgcccata tcagggccgc caccaaaccg
    13621 aaagacagcc aagtccccac acccaagacc gccgacgggt cgaacagcag caccgccgcc
    13681 aacgcctgcc accacgtcgc ccacgccgac aatcttcccc tgcgccaagc ccacgcaaac
    13741 gccgccaaca tcaaaacgct gcgctgcgtc ggcacggaaa aaccggcaag cagcgcgtaa
    13801 aacagcgcgc ctgcacaccc cgccgccaaa acccacgcgc gcggccgggc aggaaggcgc
    13861 ggggaacagg caagcagccg cttcgccagc cacgcaaaca ttaccgccac catcgtaacg
    13921 tgcaaacccg aaatactgac caaatgcgtc agccccaacg gccggaacgc ctgccacaat
    13981 tcggggcgca atgccgactg ttcgcccacg ctcaacgcgc gcatcagccc gatgccgtct
    14041 gaaagcccgc cgtccgcatc cgcctgctgc caattacggc tgatgcggct gcgccaaacc
    14101 gcaatccccc aaccgcttcc gccatgcagc aaaaccctgt ccgcaccgac cgtccccgcg
    14161 ccgcctattc cgttggataa tgcccacgcc tcacggttca aaccgcgcag gttcaactcc
    14221 ccgacgacag ggtgcacacg tgccgttatc cgccatctgc tcccgaccgc ccattcgcgc
    14281 cgtttgtagt ccgacagcag caaatcaaac gtccgaccac cgctgtccac agcctttgcc
    14341 gcaaactgca cacgccgccc gtccgacctc ggcatatccg ccacttccac cgtcaaaggc
    14401 acgcctgaaa cggcttctgc ccaccattgg gaagacaatg ccgcctccgt cctgaatacg
    14461 ccgtaagccg cgcccgccaa cacgcacagc atcagaccgg caaacgcaaa acgccttgca
    14521 aacacagcaa aaaccgcaaa agccgccagc caaaacagcc aatgcggcac gaccggcagc
    14581 gcaaacgaag ccgccgcacc gacaacccaa cacggcaaaa accaacgacg gaacacgtct
    14641 ccccacccca gatatttcaa aaataaacgg ataataaagc aaatccgaaa aaccgtcata
    14701 acaagatttg aaaatgatat agtggtttaa atttaaatca ggacaaggcg acgaagccgc
    14761 agacagtaca agcagtacga taaggcgagg caacgctgta ctggtttttg ttaaaccact
    14821 ataagaatgc aaacgaaaag cctcagtatt atccgagaca acggcaaact ttccatcgtc
    14881 attcccacga acccacatcc cgttattacc acgaaagcgg gaatctagga c
//
LOCUS       DAWFIE010000048        14612 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWFIE010000048 DAWFIE010000000
VERSION     DAWFIE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.048"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..146)
                     /locus_tag="WGQ71_001346"
     CDS             complement(<1..146)
                     /locus_tag="WGQ71_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ0707348.1"
                     /translation="MAFTLMRRAMIRKMPYTEDMRPGDTANPYGASKSMVERILTDIQ
                     KADPR"
     gene            complement(189..533)
                     /locus_tag="WGQ71_001347"
     CDS             complement(189..533)
                     /locus_tag="WGQ71_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEZ0707349.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEK
                     FYFHDEYQQLDL"
     gene            complement(526..1528)
                     /gene="dcm"
                     /locus_tag="WGQ71_001348"
                     /pseudo
     CDS             complement(526..1528)
                     /gene="dcm"
                     /locus_tag="WGQ71_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(1559..3832)
                     /locus_tag="WGQ71_001349"
     CDS             complement(1559..3832)
                     /locus_tag="WGQ71_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ0707350.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQNQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGTEPLDASAVHPEAYPVVA
                     KMLAQQGITAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(3896..4546)
                     /locus_tag="WGQ71_001350"
     CDS             complement(3896..4546)
                     /locus_tag="WGQ71_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0707351.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KAFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFAAK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(4543..5340)
                     /locus_tag="WGQ71_001351"
     CDS             complement(4543..5340)
                     /locus_tag="WGQ71_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0707352.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLKADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            complement(5340..6503)
                     /gene="ctrB"
                     /locus_tag="WGQ71_001352"
     CDS             complement(5340..6503)
                     /gene="ctrB"
                     /locus_tag="WGQ71_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ0707353.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(6518..7693)
                     /gene="ctrA"
                     /locus_tag="WGQ71_001353"
     CDS             complement(6518..7693)
                     /gene="ctrA"
                     /locus_tag="WGQ71_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ0707354.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            7828..8961
                     /gene="neuC"
                     /locus_tag="WGQ71_001354"
     CDS             7828..8961
                     /gene="neuC"
                     /locus_tag="WGQ71_001354"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ0707355.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTCKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKCQSEKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            8965..9651
                     /gene="neuA"
                     /locus_tag="WGQ71_001355"
     CDS             8965..9651
                     /gene="neuA"
                     /locus_tag="WGQ71_001355"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ0707356.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            9652..10701
                     /gene="siaC"
                     /locus_tag="WGQ71_001356"
     CDS             9652..10701
                     /gene="siaC"
                     /locus_tag="WGQ71_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ0707357.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            10785..12272
                     /locus_tag="WGQ71_001357"
     CDS             10785..12272
                     /locus_tag="WGQ71_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
                     /protein_id="HEZ0707358.1"
                     /translation="MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQ
                     LNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNIT
                     PKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
                     LLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFAHAG
                     GISINNNIANLQKKYQISKNDYIFVSQRYPISDDLYYKSIVEILNSISLQIKGKIFIK
                     LHPKEMGNNYVMSLFLNMVEINPRLVVINEPPFLIEPLIYLTNPKGIIGLASSSLIYT
                     PLLSPSTQCLSIGELIINLIQKYSMVENTEMIQEHLEIIKKFNFINILNDLNGVISNP
                     LFKTEETFETLLKSAEFAYKSKNYFQAIFYWQLASKNNITLLGHKALWYYNALYNVKQ
                     IYKMEYSDIFYIDNISVDFHSKDKLTWEKIKHYYYSADNRIGRDR"
     gene            complement(12768..13721)
                     /locus_tag="WGQ71_001358"
     CDS             complement(12768..13721)
                     /locus_tag="WGQ71_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707359.1"
                     /translation="MHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPLIITLFPGVVF
                     GDQASILSRMGADIVLYNNKHDFRIAEEYKKQYKLSCQNILYGYPIFRHASKGRHGEK
                     IYFIDQVKIPFKKEERIYTLKKLISLAEKYPEKEFTILLRVADKDITVHQDKHSYIEL
                     AKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVVADLGFSKSYA
                     NQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLKQVVPLQHDYQ
                     EYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            14104..>14612
                     /locus_tag="WGQ71_001359"
     CDS             14104..>14612
                     /locus_tag="WGQ71_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ0707360.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFKIVFSSSATVYGDPGKVPYTEDMQPGDTTSPYGTSKSMVERI
                     LTDIQKADPR"
BASE COUNT         4313 a         3322 c         2870 g         4107 t
ORIGIN      
        1 cgcggatcgg ctttttgaat atcagtgaga atgcgctcaa ccattgattt cgatgcgcca
       61 taaggattag cggtatcgcc cgggcgcata tcttccgtat agggcatttt acgtatcatg
      121 gcgcgacgca ttaaagtaaa cgccaaagcg cgtttatact ggttatccag tttccctata
      181 tttttacgtt acaaatcaag ttgttggtat tcatcgtgaa aatagaattt ttcggcataa
      241 cgctgactca taaaaccggc atgcgggctg cctgaaaaaa tcattgcgac atcatcgtca
      301 tttaatgttt catggttgtt ttctattaat gctaaagcaa tttggtaatt aattgccaaa
      361 atatcgttag taatcacacg atagccgcgt tttttggctt cgtaggcaaa ggaacagcct
      421 ccactaaata catctgcaac tgtatctaca tcagacggaa gctggtcaca aatccaggaa
      481 gctattttct ctttattacc gatatagtta attttcggat attgcttatt cattttcact
      541 catttttaaa atactttcag caatagcttt tgccaataaa ggcggtacgg cgttaccaac
      601 ctgctgctgc tgggcaattt tgctgccgca aaaaataaaa ttatcaggga aagattgtaa
      661 ggcagctaat tcacgaacgg ttaacgcccg attctgttca tagtgaaaaa ctttgcgcat
      721 atctcctgta atacaaacgg ctggttttgt tgctgttgta acggatgtat ttacggatat
      781 cacctgtttt cggacgtaat ggttcaggaa tatcgttacg gttacctcca tttttaacaa
      841 atgccatttt ttctaacatt tgtgccgaat gattcatagc ttcatgattt gcaacgtgtg
      901 gattgctttc gccagcagcc agttttggaa aatgtcctat tgctgatcca acagtctgat
      961 gggaaatctg caaaggttcg ggaaaggaaa ttttgccttt atccctcctc ccgataaata
     1021 tcactcggct acgtatctga ggaacaccga aatcggctgc actcagtatc ttacattcca
     1081 ccgaataacc gatattctga aatgcttgaa taatctcaat acgtgtttta cctgaattgt
     1141 gtgtatagag tcgcgctaca ttttccataa caaaaaaata tggttggaca attttaacta
     1201 ttcggacaaa ctctttaaat aaatggttgc gtgggtcatc tgtaaatgtc cgtccaatct
     1261 ttcctgccat actaaaacct tgacaaggtg gtcctccaat aatcaaatca actgcttgtc
     1321 cgttaagaca attgattaaa tcttgttcgg ttagtgtggt taaatctttt tgcagtaatt
     1381 gatgatgggg gaagttggta cggtaagtct gacaataatc agactccatt tcaacagaaa
     1441 gcaattgttg gaatccggct tgttcaaaac ccaaggatag gcctcctgct cctgagaaaa
     1501 ggtcaatata ggtaagtggt gtgtgcataa gtcaaaatcc ataaactctt cgattatttt
     1561 accgcttcag cttcgcaaac gcatccgcca tcgccgaatt ggctggggcg cggtcgttgc
     1621 gttggggttt gcggccggct gtccgttcgc ggctgcggtt ttcagacggc attttatgtt
     1681 ttgcgccgcc cggttcgtca tccaagcgca tggtcagcgc gatgcgtttg cgtgcagcat
     1741 cgacttccag cactttcact ttcaccacgt cgccagcttt caccacttcg cgcgggtctt
     1801 ggacgaactt gttggacagg gcggagatgt gcaccaagcc gtcctgatgg acgccgatgt
     1861 ccacgaacgc gccgaagttg gcgacgttgg aaaccacgcc ttcgagtatc ataccgactt
     1921 gcaagtcgct gatttcgtgg ataccttcgg caaacgatgc cgtctgaaac tcgccgcgcg
     1981 gatcacggcc gggtttttcc agttcggaca ggatgtccaa aatggtcggc aggccgaagc
     2041 gttcgtcggt gaagtcggac gctttgattt gcttcacgcg ctcgcggttg ccgatgagtt
     2101 cggcggcggt aatgccttgt tgcgccagca ttttggcgac gacgggatag gcttcggggt
     2161 ggacggcgct cgcgtccagc ggctctgtgc cgccgttaat ccgcaaaaag cctgccgcct
     2221 gctcgaaggt tttttcgccc aaacgcggta ctttcagcaa ttttttgcgg ctgtcgaacg
     2281 cgccgttttc atcgcggtag gcaacgatgt tttgggcaag ggtttgattc aagccggaaa
     2341 tccgcgccaa gagcggggcg gaggcggtat tcacgtccac gccgacggcg ttcacgcagt
     2401 cttcgaccac tgcgtccagc gatttggcga gctggttttg gttcacatcg tgctgatact
     2461 ggcccacgcc gatggattta gggtcgattt tgaccaactc ggcaagcggg tcttgcagcc
     2521 tgcgggcgat ggacaccgcg ccgcgcaggg aaacgtccaa gtcggggaac tcgcgcgccg
     2581 ccagttcgga cgcggaataa atcgacgcgc cggcttcgga cacgacgatt ttatgcagcc
     2641 ccattttcga catttcacgc accagttcgc ccgcgatttt gtcggtttca cggctggcgg
     2701 tgccgttacc gatggcgatg agcttcacgc cgtgctgttt aatcaggcgc gacagcgttg
     2761 ccaacatatt gttttcttga tgcaaataga cgatgacggt atccagcagc ttgccggtgt
     2821 cgtccaccac ggcgcatttc acgccgttgc ggtagccggg gtcgagaccc agcgtggtca
     2881 gccgtccggc gggcgcagca agcagcaagt ctttgagatt gcgggcgaac acggtaatcg
     2941 cgtcggtgtc ggcggcttct ttcaaacgat tgagagcttc aagttccaac gacaaaaaga
     3001 ttttcgcgcg ccaagtcagg cgcacggtgt cgcgcagcca tttgtggccg tctgaaactt
     3061 tgaagcggcg ggcgatgatt tgctcgtatt cgctttgctg cgtaatcggc gtgtcgtcgg
     3121 gctgatattt gagcgcgatg ttcaacacgc cttcgttgcg tccgcgcaaa accgccagcg
     3181 cacgatggct gggcatcgca cgaataggtt cgcggtggtc aaaataatcg ctgaattttt
     3241 cgccttcggt ttctttgcct ccaacaactt gcgcgtggat ttcggcttcg ttccacagct
     3301 tgtcgcgcag cgtgccgatg agttccgcgt cttcggcaaa ctgctccatc agaatcgcac
     3361 gcgcgccgtc caacgcggct ttggcatcgg ggacgttttc gttcaggtag ccttgtgcgg
     3421 cggcttccac gtcctgcgac tgctcggcaa gcaacacgtc cgccagcggc tgcaaaccgt
     3481 gttcgcgcgc gatttgcgct ttggtgcggc gtttgggttt gtagggcaga tacaggtctt
     3541 ccagcgcggt tttgttatcg gcggcttcga tttgcgccct gaggtcgtct gaaagcttgc
     3601 cttgctcttc aatgcttttt aaaacaacgg ctttgcgctc ttccaactcg cgcagatatt
     3661 gcagccgctc ggcaagccgg cgcaactgcg tatcgtccaa cccgcccgtc gcttccttgc
     3721 ggtagcgggc gataaacggc acggtcgcgc cgtcgtccaa aagctcgacg gcggcggtga
     3781 tttgcgcggc agtcgcggag agttcttggg aaagaatttg agtaatgttc atcaatagaa
     3841 ttccaacgga caggccgtct gaaatttcag acggcctgat ttaaaaacaa tcgctttaag
     3901 gcagcgaatt ataatattcg taggctttgt ccatatcttc aaactggtac atatgccctt
     3961 tttccagcac catcgcatta tcgcaatatt gcttcatggc gctgtggctg tgcgacacca
     4021 agatgatgga acggtctttg cgtttttcaa acagctcata tttacattta gctgcaaaac
     4081 gtgagtcgcc caccgcaatc acttcgtcaa tcaggtaaca gtcaaactcc accgccaacg
     4141 acagcgcaaa agccaaacgc gctttcatac ctgaagaata gcgtttcacc ggctcataca
     4201 aatattgccc cagctccgaa aattcttccg taaacgcttt cacataatcg atatcgacat
     4261 tataaatccg gcagataaaa cgcagattat ccatcccggt caggcttcct tggaaggcac
     4321 cggaaaacgc cagcggccaa gaaatactca ttgtccgctt gatttcaccc gtggtcggcg
     4381 gctcaacgcc actgatcaaa cggatgagcg tcgatttacc tgcaccgttg cggccgagaa
     4441 taccgatttt ctcgcccttc tccattttga agctaatatc gtgcaagact gtccgccaac
     4501 cttggcgggt cagatagcgt ttggaaacgt gttcaactga aatcattgcg gctcaacccc
     4561 tttgctgaat ctcgccacca ccgccaaacc aagcagcagc aaaaccaagt tacacaacaa
     4621 aatataccaa ggattttcgt aagtcgtgac gctgtcgcca aaatatccgg cacggaacat
     4681 ttctgtgcca tgcaccatcg ggatcatcaa tgcatactct tgcactttcg gaggcaaatt
     4741 atgtacaaaa aagaatgcac ccgataacgg catcatcaca aaagtcaatg tgccccaaat
     4801 cttgccaaac ggctcgaaat taaaggcaat cgaacaaatc accaaaccca aaccaatcgc
     4861 aaaaaaagcc atcaaaagcc aagccatcag catataaaac atatctgccg gcatttcaat
     4921 ccagccaatc gcaatcaata ccgccataat cacaatctgc gcaatggttg caccagcaat
     4981 ttccaaaatc atgcgcgcca agatggtatc caaaactctt acattgcggt gataaagcaa
     5041 gctggcattt gaagaaatcg acccaactgc ccgttttgag gcattacgcc acatcatcaa
     5101 catcggatag ccagtaatcg caaatgcgac aatattcaaa gttgaatatc ggtctgcctt
     5161 taaaaatttc cacatcaaga cgataacgaa tgtcatcagc aacggctcaa caaacagcca
     5221 taaaaagcca atattattgc gaccgtaacg ggtgataatt tcccgcatca aaagcgcacc
     5281 gattacgcgc ctttgaatgg ctaaagattc ccaaaatgat gttttatgca aggctttcat
     5341 cagtttttat gctcacgaat gctggcagtc aacaggctca aaataccata aaccatcaag
     5401 ccgataatca gagtggcaac aatgttgtat aaccgtttag gctcatgtgc caaatccggc
     5461 aggctcggtt gcgagatcac ttccaaataa agctgctgac ggtctgcttc aaccttggca
     5521 ctttccaaag aagtcatggc ggctgccaac tgctgctctg ccaactggtt ttccaaatac
     5581 acacgctgat attcggcagc ctgattagac aacgaagaat gcccaccgcc cgaaatggca
     5641 cgtaactgtt ggtcaatttc tttacgcaag ctctgctcac gcgcctgcaa acccggaatc
     5701 tgcggattct ccggagtgac tgctttcacc tgatccagct gggtttgaat cacaatcaat
     5761 tcatcttgca gcttggaaac caaccccatt tgcacttccg attgcgcttt caaatcaaaa
     5821 acgccattgg caatccggta atccgtcaga ttctgagagg cttcctttac ccgctctgcc
     5881 gccgttttca ctacttcttc cgcatagcgc accgtatcag cacgtgcacg atcgttcaac
     5941 tggttaatca atgcttcacc ttgttttaac aaagcctcat tgattttctt agattccagc
     6001 gcatcaaagg aagttacatt caacgtggaa atacccgaaa ccgtatcaaa attgatcatc
     6061 acctgatttt tatagtattg ataaaaagcc tcttcctcgc cacggaaccc aaacccatta
     6121 aagcggctga acgcatcacc tttggtttca taaaactcac gcaccggcaa gattttacgc
     6181 agttcatcca aagacgagcg cgaacgcata tactccccaa ccgtgtaaat atcatcttgc
     6241 gcacgggcaa aacctgtgcc ctgcaaaatg gcacccaggc cattgagaga agattggctt
     6301 ttaggcgagc gcaccacaaa gctcgattgc gacgtaaaac gatcggaagc gaagaagccg
     6361 aaatacacca acgaaattac cgtagggata atcaccgtta cccaaaataa agggcttagc
     6421 tttttaatcc aacttttctt tttcggcttt ttacgctcgg ctttggtttc agtggcaact
     6481 gccacaggaa gttgctcaga catcttaatt actcacatta attagttaaa ttattaatac
     6541 tgttcgcgcc actggtaacc ggcgagaaca caaacgacaa gaatttctgc acttcagcca
     6601 acggcgcatt cgacacatac aatacatctt tattcttcac aggaaagcgc tgcatagaaa
     6661 atagcgaatg cgcatcagcc atattcacac gatataccgt tggaatctct gcctcactgc
     6721 cataaccttg agcaatccat ttatcctgac gttctgccgg caattccacc aatggcgtat
     6781 agcggaacac aaacacacca cgcgcatcag aacggcgatc ttgcaaaccg cccatacggc
     6841 caatggcttc agaaagcgat aagcctctgg ctgaaaaacc gatttcttgt gttctcccca
     6901 ccgcacccat agacgtaaag gtatagggat tggtaatcat ggtaaccaca tcaccgcgac
     6961 gcagcaaaat attttgtcgc ggatttgcaa ctaaatcttc caaggcaaca gttcgtacta
     7021 cattgccacg tgtcagctgc acattcgtat cctgcacatt tgccgttgaa ccacctaccg
     7081 cagccaccgc atccaacaca cgctcaccgg ctgccgtcag cggcatacgc acactattcc
     7141 cagcacgaat caccgacaca ttcgccgcat tattctgcac caaacgcacc atcacttgtg
     7201 gctgattggc catttttttc aggcggcctt taataatttc ctgaacctga ccaggcgttt
     7261 taccgaccac cgaaatatcg ccaacaaacg gcacagaaac cgtaccacgt gccgtgacca
     7321 actgctctgg caacttagtt tgatgcgcac tacccgagcc catcgaagaa aggccaccac
     7381 caaacaatac tgccggcggc gcttcccaaa tcataatatc caatacatca ccaatattta
     7441 gcgtaccagc cgaagcataa ccatcgccaa actgagtgaa tgactgattt atctgagcct
     7501 tatataataa ctgagcaacc gtatgattca catcaatcag ctccacttca ggaatttgaa
     7561 cttcagattg ttgccctaaa gagacaattt tttttgcgct ggggcctgat gaaggaatcg
     7621 cagagcatcc tacaattaaa cttccacaca ataataatac tgcgtgacga atataaaatt
     7681 tcactttaaa cacaagccaa atcctaatat aattataaat ggcctaatta tagcacttaa
     7741 tcgaaataaa tttatgagta cgtagagtat aattagtatt cttctttcca acttccttat
     7801 acttatatac ttatagattc taaaatcatg aaaagaattc tttgcattac aggtaccaga
     7861 gccgacttcg gcaagctaaa acctttatta gcctatattg aaaatcaccc agaccttgaa
     7921 ttgcatttga ttgtaactgg tatgcatatg atgaaaacat atggcagaac ctgcaaggaa
     7981 gtaactcgag aaaactatca acatacatat ctgttttcaa atcaaatcca aggtgaacca
     8041 atgggtgccg ttttaggcaa taccattacg tttatctctc gtctatctga tgaaattgaa
     8101 cctgatatgg tcatgattca cggcgaccgt ttagaagcac tagcaggcgc aactgtaggt
     8161 gcattaagca gccgtttagt ttgccatatc gaaggtggtg aactatctgg tacagtagat
     8221 gactccattc gtcattctat tagtaaactt tctcatatcc acttggtagc aaatgaacaa
     8281 gctgtcactc gcctagtgca aatgggagaa aaaagaaagc atattcacat catcggctcc
     8341 cccgatttag atgttatggc ctcttccacc ctcccatcct tagaagaagt caaagaatat
     8401 tacggtttac catacgaaaa ttatggtatt tctatgtttc accccgtgac tacagaagca
     8461 catttaatgc cacaatatgc ggcccaatat ttcaaagcat tagaattaag tggccaaaat
     8521 atcattagca tttaccctaa caacgacacg ggtactgaaa gtattttaca agagttatta
     8581 aaatgtcaat ctgaaaaatt tatcgcattt ccatctatac gatttgaata ctttttagtc
     8641 ttattgaaac atgctaaatt tatggtcggc aactcaagtg caggtattag agaagctcct
     8701 ctctacggtg tcccttcaat tgatgttggt acacgccaaa gtaaccgcca tatgggaaaa
     8761 tctattattc atacagatta tgaaactaaa aatatctttg atgcgattca acaagcatgc
     8821 agtttaggca aatttgaagc agatgatacc tttaatggcg gagatactcg caccagcaca
     8881 gaaagatttg ctgaagtaat caacaatcct gaaacgtgga atgtttctgc tcaaaaacgt
     8941 tttatcgatt tgaatcttta aattatggaa aaacaaaata ttgcggttat acttgcgcgc
     9001 caaaactcca aaggattgcc attaaaaaat ctccggaaaa tgaatggcat atcattactt
     9061 ggtcatacaa ttaatgctgc tatatcatca aagtgttttg accgcataat tgtttcgact
     9121 gatggcgggt taattgcaga agaagctaaa aatttcggtg tcgaagtcgt cctacgccct
     9181 gcagagctgg cctccgatac agccagctct atttcaggtg taatacatgc tttagaaaca
     9241 attggcagta attccggcac agtaacccta ttacaaccaa ccagtccatt acgcacaggg
     9301 gctcatattc gtgaagcttt ttctctattt gatgagaaaa taaaaggatc cgttgtctct
     9361 gcatgcccaa tggagcatca tccactaaaa accctgcttc aaatcaataa tggcgaatat
     9421 gcccccatgc gccatctaag cgatttggag cagcctcgcc aacaattacc tcaggcattt
     9481 aggcctaatg gtgcaattta cattaatgat actgcttcac taattgcaaa taattgtttt
     9541 tttatcgccc caaccaaact ttatattatg tctcatcaag actctatcga tattgatact
     9601 gagcttgatt tacaacaggc agaaaacatt cttaatcaca aggaaagcta aatgcaaaac
     9661 aacaacgaat ttaaaattgg taatcgttca gtaggttaca accacgaacc attgattatc
     9721 tgtgaaatcg gcatcaatca tgaaggctct ttaaaaacag cttttgaaat ggttgatgct
     9781 gcctataatg caggcgctga agttgttaaa catcaaacac acatcgttga agacgaaatg
     9841 tctgatgagg ccaaacaagt cattccaggc aatgcagatg tctctattta tgaaattatg
     9901 gaacgttgcg ccctgaatga agaagatgag attaaattaa aagaatacgt agagagtaag
     9961 ggtatgattt ttatcagtac tcctttctct cgtgcagctg ctttacgatt acaacgtatg
    10021 gatattccag catataaaat cggctctggc gaatgtaata actacccatt aattaaactg
    10081 gtggcctctt ttggtaagcc tattattctc tctaccggca tgaattctat tgaaagcatc
    10141 aaaaagtcgg tagaaattat tcgagaagca ggggtacctt atgctttgct tcactgtacc
    10201 aacatctacc caacccctta cgaagatgtt cgattgggtg gtatgaacga tttatctgaa
    10261 gcctttccag acgcaatcat tggcctgtct gaccatacct tagataacta tgcttgctta
    10321 ggagcagtag ctttaggcgg ttcgatttta gagcgtcact ttactgaccg catggatcgc
    10381 ccaggtccgg atattgtatg ctctatgaat ccggatactt ttaaagagct caagcaaggc
    10441 gctcatgctt taaaattggc acgcggcggc aaaaaagaca cgattatcgc gggagaaaag
    10501 ccaactaaag atttcgcctt tgcatctgtc gtagcagata aagacattaa aaaaggagaa
    10561 ctgttgtccg gagataacct atgggttaaa cgcccaggca atggagactt cagcgtcaac
    10621 gaatatgaaa cattatttgg taaggtcgct gcttgcaata ttcgcaaagg tgctcaaatc
    10681 aaaaaaactg atattgaata atgcttatta acttagttac tttattaaca gaggattggc
    10741 tattacatat agctaattct cattaatttt taagagatac aataatgcta aagaaaataa
    10801 aaaaagctct ttttcagcct aaaaagtttt ttcaagattc aatgtggttg acaacatctc
    10861 cattttatct taccccccca cgtaacaatt tatttgtcat atctaattta ggtcagctta
    10921 accaagtcca aagcctaatt aaaatacaaa aattaaccaa taatttacta gtaattttat
    10981 atacttctaa aaacttaaaa atgcctaagt tagttcatca atcagctaac aagaatctat
    11041 ttgaatctat ttatctattt gagcttccta gaagccctaa taatataact cctaaaaaat
    11101 tactttatat ttatagaagt tacaaaaaaa tccttaatat tatacagcct gctcatctct
    11161 atatgctgtc ttttacaggc cactactcct atctgattag tattgcaaaa aaaaagaata
    11221 ttacgactca tttaattgat gaagggactg gaacatatgc tcctttatta gaatcatttt
    11281 catatcatcc aacaaaatta gaacgttatt tgattggaaa taatcttaat attaaaggat
    11341 atatagatca ttttgacata ttgcatgtcc cctttcctga atatgctaaa aaaatattta
    11401 atgcaaaaaa atataaccgg ttttttgcgc atgctggagg aataagcatt aataataaca
    11461 ttgcaaactt acagaaaaaa tatcaaatat ctaaaaatga ctatattttt gttagtcaac
    11521 gctaccccat ttcagatgat ttgtattata agagtatagt agaaatctta aacagcataa
    11581 gtttacaaat taaaggaaag atatttatta aactacaccc aaaagagatg ggcaacaact
    11641 atgtaatgtc tttatttcta aatatggtag aaataaaccc tcggctggta gttattaatg
    11701 aacctccttt tctaattgag cccctaatat acttaacaaa tcctaaagga attataggcc
    11761 tggcctctag ttctttaatt tatacaccat tactctcacc ctcaacccaa tgtctttcta
    11821 ttggagagtt aattattaac ttaattcaaa aatattcaat ggtggaaaac actgaaatga
    11881 tccaagaaca cttagagatt attaagaaat ttaattttat taatatacta aatgatttaa
    11941 atggggtaat aagtaaccct ctctttaaaa cagaagaaac atttgaaaca cttcttaaat
    12001 ctgcagaatt cgcatataaa tctaaaaact actttcaggc tattttttac tggcaacttg
    12061 ccagcaaaaa caatattacc ttattagggc ataaagcatt atggtactac aatgcacttt
    12121 ataatgtaaa acaaatttat aagatggaat attcagatat tttttatatc gataatatct
    12181 ccgtagactt tcatagtaaa gataaattga catgggaaaa aattaaacat tattactatt
    12241 ccgccgacaa tagaattggt agagatagat aaaaatacta tgacttctga tctccatacg
    12301 tatgcttgtt ttcaaaaagc agcattaaga tagataagtg aagcttgatg agagccattt
    12361 ttgtggtcat cgccaaggta cacgcggaga gtaatcgaca acagagctgc tgtattctgg
    12421 cttttaaagc ccagtagtaa agtttataag gctgcatttc tcaatgtaaa acgcgatatt
    12481 ttcacccatt attagggata agtagccagt ggtttacact ggctactttc atagttatga
    12541 agtactgggt ctgagtgaat tcaattattt ccgtataaaa cacagtagac attttactaa
    12601 tcggcaaaat tatattagaa gaattgagaa ctactgaagt caggtaagat accattttgc
    12661 acaaatttaa cggcatccct aaaaagcatt ttgggtggta tttaaaggag tacaaaatgg
    12721 ttttaactac agtatgctta gaaatctata gccttacgcg ctttatatta aatcaccttt
    12781 ctcaaccatt tagaatcata aaaaaacttt ttaggatccc taattaactt tttaaatttt
    12841 cttaaaaaaa tagttttagt tgattctata ctattcaccg cagataaata ttcttggtaa
    12901 tcatgttgca atggcacaac ctgctttaat agcttattaa actcatcagt tgtaatcacc
    12961 tttttagtag catagcaatc aagccaatcc ggatctgcta tttttgggga agtgaaatct
    13021 atttgatcaa aataaactaa aaccccacta cctaaaaaat gctgatttgc ataggattta
    13081 gaaaagccta agtctgcaac aacaccaaca gggataccct tacattcagc ttcaaaaagc
    13141 atagtagatg aataagataa acaatacccc atttcttgga aggcttgcgc ggtacttttt
    13201 cgctctattg tcaaattact cggcaactga aactgctttg ccagctctat atacgaatgt
    13261 ttatcctgat gcacagtaat atctttatct gcaaccctta gcaaaatagt aaattctttc
    13321 tcagggtatt tttcagccaa ggaaatcaat ttttttaatg tataaattct ttcttctttt
    13381 ttaaatggga ttttaacttg gtcaataaag taaattttct ctccatgacg accttttgat
    13441 gcgtggcgaa aaattggata accataaagt atattttgac aacttaattt atattgtttc
    13501 ttatattcct ctgcaattct aaaatcatgc ttattattat ataaaacaat atcagccccc
    13561 atacgagata gaatacttgc ctgatcaccg aatactacac ctggaaacaa ggtaataatc
    13621 aatggccttg aagcaatatt taattgcgca ttctgcttaa agaacctttt taacaaccca
    13681 tttccaaccg ataaaattac tgcgtcataa tattgatgca tattcttaat aaagtaatca
    13741 tcaatatgaa aaaaaaaatt gcttggtctt tatctatccc tgattctaat aactgtcgat
    13801 ttgaaagaat gttttctcga cttttgtgga tataaatatc aatttgagca tcttttatct
    13861 cttttgcaac agcatagccc gagttaagga acgagtcata actggcaatt agtaatactt
    13921 ttttcactat tttcccttaa ttctacacca tatgaataaa attatagtat tttaccttag
    13981 atatcaaata tacttaacca aaaaaatctg aagaaatcta ctgctacttc agggataatt
    14041 atttcattaa gattttacaa ccattattat caatctatca aagtattttt agtattttat
    14101 tctatgaaaa aaattctcgt taccggaggc accggtttta tcggctcgca taccgttgtt
    14161 tctttgctga aaagcggcca tcaagtcgtg attttggata acctatgcaa ttccagcatc
    14221 aatatcctgc cacgcttgaa aacgattacc ggccaagaga ttccgtttta tcaaggcgat
    14281 atccgcgatc gtgagatttt gcgccgtatt tttgcggaaa accgcattga ttcggtgatt
    14341 cattttgccg gcttgaaagc ggtgggtgaa agtgtggccg agccgatgaa atattatgat
    14401 aataatgttt ccggcagctt ggtgttggcg gaagaaatgg cgcgtgccgg cgtatttaag
    14461 attgtgttca gctcatcggc tacggtttac ggcgatccgg gcaaagtgcc ttatacggaa
    14521 gatatgcaac cgggggatac gaccagccct tacggcacat ctaagtctat ggtggagcgc
    14581 attttgacgg atatccaaaa ggccgatccg cg
//
LOCUS       DAWFIE010000049        14550 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWFIE010000049 DAWFIE010000000
VERSION     DAWFIE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14550
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.049"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..144
                     /locus_tag="WGQ71_001360"
     CDS             <1..144
                     /locus_tag="WGQ71_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ0707361.1"
                     /translation="NVGIGSGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRH
                     IQT"
     gene            274..1710
                     /locus_tag="WGQ71_001361"
     CDS             274..1710
                     /locus_tag="WGQ71_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ0707362.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     HALNIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLSFVVFNTASPD
                     DAGAVFNALFANANGWNAPQQANMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            1721..2704
                     /locus_tag="WGQ71_001362"
     CDS             1721..2704
                     /locus_tag="WGQ71_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ0707363.1"
                     /translation="MKNFLSLFTSILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYTLSDGIKAFLSGETPPTAQDGGSADMPSEAATSEAVPQTGET
                     EWKQDTDTAAVRSGDKVFFVGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLQKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     HTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2701..3891
                     /locus_tag="WGQ71_001363"
     CDS             2701..3891
                     /locus_tag="WGQ71_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ0707364.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRYNGNWQSLNSRSAAADFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAKR"
     gene            complement(3904..4083)
                     /locus_tag="WGQ71_001364"
     CDS             complement(3904..4083)
                     /locus_tag="WGQ71_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707365.1"
                     /translation="MVADGERPHLKTICRLKTEGGSLSSLPSDGISNAAFAPYAGCGR
                     IRLVMATRARGGYAV"
     gene            4096..5649
                     /locus_tag="WGQ71_001365"
     CDS             4096..5649
                     /locus_tag="WGQ71_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ0707366.1"
                     /translation="MNRTYANFYEMLTAACRKNGNGTAVFDGKEKTTYDALKQEVEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFMFASAGLSKELAGLKAQTPVEKIIWTGKSRQGGGAAEGDAFFEDVRRFPEKPDL
                     GHQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEVKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(5740..7767)
                     /locus_tag="WGQ71_001366"
     CDS             complement(5740..7767)
                     /locus_tag="WGQ71_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ0707367.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQAQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGVLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            8056..10062
                     /locus_tag="WGQ71_001367"
     CDS             8056..10062
                     /locus_tag="WGQ71_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ0707368.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGVIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHILHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLHQQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            complement(10290..11381)
                     /gene="mltB"
                     /locus_tag="WGQ71_001368"
     CDS             complement(10290..11381)
                     /gene="mltB"
                     /locus_tag="WGQ71_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ0707369.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRVNEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAVIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(11530..12957)
                     /locus_tag="WGQ71_001369"
     CDS             complement(11530..12957)
                     /locus_tag="WGQ71_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0707370.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDTAAFLLNDIRKDILDCRYCG"
     gene            13142..>14550
                     /locus_tag="WGQ71_001370"
     CDS             13142..>14550
                     /locus_tag="WGQ71_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0707371.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLSAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSSDVFAGRLKNYGQVLP
                     DIFGVEHTLPNVAVNRQADEPLQALKDFQTAFKGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSAAATNPLPSPLPQEREQ"
BASE COUNT         3368 a         4388 c         3662 g         3132 t
ORIGIN      
        1 aacgtcggta tcggcagcgg cgcactgttg ggacattggg taacgcaata tgtcggcatt
       61 tcccgtatcg gcatcgccgg tatgcttgcg gcggcggccg ggctgtggct gtgcctgaac
      121 ttgaaccgcc atatccaaac ctgaccgcgc gatgccgtct gaagcccctc ccgcccttca
      181 gacggcattt tgattgaaga aatatccgcc cctgcttaaa ataacgggct ttgccgtgtt
      241 ttaacgccta ttttcttatt ccaaggatgg tttatgccgc tgctgtctgt cgagttcgca
      301 ctgttctttc tcgccttcct gccgatttac tggggcttgg cgaaataccc gtccgtccaa
      361 aacctgctgc ttttggctgc cggtatgggc tggctctacc atatcggccc cgtatttgcg
      421 gcaatcgtcg tcctttattc ctcctgcgtg taccttttgg gcgaactgct ccgttccgat
      481 cgcgaaaata cgcgccgctt ttggctgggg tgcggcattg ccgtctcgct gaccgtcttg
      541 ggctttttca aatatttcga ctttttccgc ccgctgattg cacaatatgc cggacaaggc
      601 ggcgcaatcg acatcctgat gccgctgggg ctttcgtatt acaccttcca gtcgctcgcc
      661 tatctggttt actgcttccg cgccccgcac gccgcgcgtt tcgagtggca cgagctgctg
      721 ctgcacctga gttttttccc caccgttacc tccggcccga ttatccgcgc cgccgcattc
      781 aaaagcgcag acggcgagca ggcgggcgca ttggcgcaaa tccgtacccg ccaaccgcgc
      841 tcacccgtcc gccccgcact cgccgtttcc ctgattctgc tgggtattgc caaaaaatgg
      901 tggctggcgg ggatgctggc ggaaaactgg gtgtcgcccg tatttgaaaa tcccgcccaa
      961 ttcgacggct ggggcgtatt gggcggcgtg tacggctata ccttccaact ctttttagac
     1021 ttttccggat attccgattt ggttatcggc atggcgatgc tgctgggttt ccggctgccc
     1081 aaaaacttct ccgcaccgct tcatgcttta aacatccgcg cattttggga caaatggcac
     1141 atcagccttt ccacctggat acgcgactac atctacatcc ccttgggcgg cagcaaaaaa
     1201 ggctttttac ggacacagct caacctgatg gcggcaatgg tactctcagg catctggcac
     1261 ggctacggct ggaacttcct catttggggc gcgctgcacg gcacggcact ggtgctgctc
     1321 aacacgggcg atcgctattt cggacgcaac gcgctatgcc gtctgaaata ccttgcgccg
     1381 ctctcgtggt ttgttacctt ccatttcgtc tgccttagct ttgttgtctt caataccgca
     1441 agccccgacg atgcaggcgc agttttcaat gccctctttg ccaatgccaa cggctggaat
     1501 gcgccgcaac aggcaaacat gctgctgctt gcctcgtttg catccgtgat gctgctctac
     1561 ccttacctgc aacgcgcttt cgacggcgcg gtcaaaggtt tggaaaaaat cccgatgtgg
     1621 ctgtggttta tccccgtttc cgccgtcctg ctgctgatta tcgtattggc cccctcgggg
     1681 attcccggct ttatttatgc caatttttaa gggtttggac atgaaaaact ttctttccct
     1741 tttcacttcc atattgatgt ctgccctgat tgccgtgtgg ttcagccaaa accccatcaa
     1801 cgcctactgg cagcagacct accaccgcaa cagcccgctc gaaccgcttg ccgcctacgg
     1861 atggtggcgg agcggtgcgg cattgcaaga aaacgcctac accctttcag acggcatcaa
     1921 agccttcctg tccggcgaaa cgccgccgac ggctcaagac ggcggctcgg cagatatgcc
     1981 gtctgaagcc gccacatccg aagccgtccc tcaaaccggt gaaacagaat ggaaacagga
     2041 caccgacacc gccgccgtcc gcagcggcga caaagtcttt tttgtcggcg actcgctgat
     2101 gcagggcgtt gcccccttcg tgcaaaaaag cctgaaacag caatacggca tcgaatccgt
     2161 caacctcagc aaacaaagca cggggctgtc ctacccctca ttcttcgact ggccgaaaac
     2221 gattgaagaa accctgcaaa aacatcccga aatcagcgtg ctcgccgtct tcctcggccc
     2281 gaacgacccg tgggatttcc ccgtcggcaa acgctacctc aaattcgctt ccgacgaatg
     2341 ggcgcaagaa tacctgaaac gcgtcgaccg catccttgaa gccgcacaca cgcaccgcgt
     2401 ccaagtcgtt tggctcggca tcccctacat gaaaaaagcc aagctcgacg ggcagatgcg
     2461 ctacctcgac aaactgcttt cggaacactt ggaaggcaaa atcatcctga ttcccaccgc
     2521 gcacaccctg agcggcgggg aagaccgcca caccgactcc gtcaacgtca acggcaaacc
     2581 cgtccgctac cgcagcaagg acggcataca ctttaccgcc gaaggacaaa aactgctggc
     2641 ggcaaaaata atggaaaaaa tcgtttttga accaagtacg caaccatcaa gtacacagcc
     2701 atgaacccca aacacctcat cgcattttcc gccctgttcg ccgccacgca ggcagaagcc
     2761 ctgcccgtcg cctccgtcag cctcgacacc gttaccgttt ccccatccgc tccctacacc
     2821 gatacaaacg ggctcttgac cgactacggc aacgccgccg cctcgccttg gatgaaaaaa
     2881 ctccgatccg tcgcacaagg cagcggcgag accttccgca tcctgcaaat cggcgactcg
     2941 cacaccgccg gcgacttctt taccgacagc ctgcgcaaac gcctgcaaaa aacttggggc
     3001 gacggcggca taggctgggt ttaccccgcc aacgtcaaag ggcagcgtat ggcggccgtc
     3061 cgctataacg gcaactggca aagcctcaac agtcgaagtg ccgcagcaga cttcccgctc
     3121 ggcggcatcc tcgcccacac cggcagcggc ggcagcatga ccctgaccgc atcggacggc
     3181 aagaccggca aacagcgcat ttccctgttt gccaaacccc tgctcgccga acaaaccctg
     3241 accgtcaacg gcaacaccgt ctccgccaac ggcggcggct ggcaggtact ggatacccgc
     3301 gcggcactgc ccttgaccat acagaccgaa atgccgtggg acatcggctt catcaacatc
     3361 gaaaatcccg ccggcggcat taccgtttcc gccatgggca tcaacggcgc acaattgacc
     3421 cattggtcga aatggcgtgc cgaccgtatg aacgaccttg cccaaaccgg cgccgatttg
     3481 gttatccttt cctacggcac caacgaagcc ttcggcgaca acatcgacat tgccgacacc
     3541 gaacaaaaat ggctggatac cgtccgccaa atccgcgaca gcctgcctgc cgccggcatc
     3601 ctcatcatcg gcgcacccga atccctgaaa aacacgctcg gcgtatgcgg cacacgcccc
     3661 gtccgcctga ccgaagtcca acagatgcag cggcgcgtcg cccgacaggg acaaaccatg
     3721 ttctggtctt ggcaaaacgc catgggcggc atatgcagca tgaaaaactg gctcaaccaa
     3781 ggatgggccg ccaaagacgg cgtacacttc tccgccaaag gctaccggcg cgcggcggaa
     3841 atgctcgccg acagcctcga agaactcgtc cgcgccgccg cgaaacggta aacaaaaagg
     3901 ctttcagacg gcatagccgc cccgcgcacg agttgccatg acaaggcgga ttcgtccgca
     3961 accggcatac ggcgcaaagg cggcgttcga tatgccgtct gaaggcaaag atgataaact
     4021 gccgccttcc gttttcagac ggcatattgt tttcaaatga gggcgttctc cgtccgcaac
     4081 cataaaggaa gtttcatgaa ccggacttat gccaatttct acgaaatgct gaccgccgcc
     4141 tgccgcaaaa acggcaacgg tacggcagtg ttcgacggca aggaaaaaac cacttacgat
     4201 gcgctcaaac aggaagtaga agccgttgcg gcgtacctgc aaaatatcgg cgtgaagttc
     4261 ggcgacacgg tcgcgctggc ggtttccaat tccacagaat ttattaccgc ctatttcgcc
     4321 gtctccgcca tcggcgcggt tgccgtgccg atgaacacat ttttgaaaaa cagcgaatac
     4381 gcatatattt tgaacgactg caaggcgcgg tttatgttcg cctcggccgg cctgtcaaaa
     4441 gaattggcag gcttgaaggc gcaaacgccc gtcgaaaaaa tcatttggac aggcaaaagc
     4501 cgacagggtg gcggagcggc ggaaggcgat gccttttttg aagacgtgcg ccgtttcccc
     4561 gaaaaacccg acttgggcca ccaaccccgg ataaatgatt tggcacacat catctacacc
     4621 tccggcacga cggggcatcc caaaggcgcg ctaatcagtt acgccaatct gttcgccaac
     4681 ctgaacggca tcgaacgcat ctttaaaatc tccaagcgcg accgctttat cgttttcctg
     4741 ccgatgttcc acagcttcac gctgacggct atggtgctgc tgccgattta tatggcgtgt
     4801 tcgattattt tggtcaaatc tgttttcccg ttttccaacg ttttgaaaca ggcactgctc
     4861 aaacgcgcga ccgtgttttt gggcgtgccc gcgatttaca ccgcgatgag caaggcgaaa
     4921 atcccttggt atttcagatg gttcaaccgc atccgcctgt ttatcagcgg cggtgcgcct
     4981 ttggcggaac aaaccatcct cgatttcaaa gccaagttcc cccgcgccaa attgctggaa
     5041 ggctacggac tgagcgaagc ctcgcccgtc gtcgccgtca atacgcccga gaggcaaaaa
     5101 gcccgcagcg tcggcatccc cctgcccggt ttggaagtca aagccgtcga tgaagaattg
     5161 gtcgaagtgc cgcgcggcga agtgggcgaa ctgattgtca ggggcggttc ggtgatgcgg
     5221 ggctacctca atatgcctgc cgccaccgat gaaaccatcg tcaacggctg gttgaaaacg
     5281 ggcgatttcg ttaccataga cgaggacggc ttcatcttta tcgtcgaccg caaaaaagat
     5341 ttgattattt ccaaaggtca aaacgtctat ccgcgcgaga tcgaagagga aatctacaaa
     5401 ctcgatgccg tcgaagccgc cgccgtcatc ggcgtgaaag accgttatgc cgacgaggaa
     5461 atcgtcgcct tcgtccaatt gaaggaaggt atggatttgg gcgagaacga aatccgccgc
     5521 cacctgcgta ccgtgctggc aaatttcaaa atccccaaac aaatccactt taaagacggg
     5581 ctgccgcgca acgctacggg caaggtattg aaacgggtgt tgaaggagca gtttgacgga
     5641 aacaaatgaa cgccgtgccg tccgaagccc cgtccggcaa aaaaaatgcg gtgaatctgc
     5701 ttcaccgcat ttttttggca aacgccggag cgaggatatt tactctgcca attccacctg
     5761 ctcgtgcgcc atcagttcct gaccgacatc atccaacaac atcaaataac gttcgtagtt
     5821 tttcaaaatg tcggcaatca gctcgtcctt gttcatatac tgcacatcgt acccgacgcg
     5881 cccgtcgaaa aaataagcgt agggtttgta agttgtctgg tgccggatat gcggcagctt
     5941 gccgtcgtta atcaactggt cggatacatc ctgcccgaca gacttaatcc cgtacataaa
     6001 atcgcgcatc gtctctttcc gaatgacgaa ctcgattgcg ggctcgtcct gatgaaacat
     6061 cttatcgacc cggacgctca agccgtattc ttccgaaagc tcccgttgca actcgtgcat
     6121 agcgggcgat gcggtatgtt tgaggaattt taaaatatcc tgctcctgcg tctggctcat
     6181 tatctgcacc agccgttctt tccacttgcc gcccgtccaa aatacactgg tagggttaac
     6241 ccgggtctca aaatatttct tatccgcact caatcctttc cacaggctga aacacattat
     6301 cagcatcagc agggcaaacg gcagggaaac aatcagggtc atagactgca ggttgccgag
     6361 tccgcccgag cgcatcagca aaacggcaac ggcagacatc agcacgcccc acataaccgc
     6421 ctgccaccgt ggcgcgctca agcctttgtc ccgagaggta atattgttca ggacataaat
     6481 cccggaatcg gcagaagtta caaaaaacag agaaatgacc agcaggctga cgatgctcgt
     6541 cagttcgggc agagggaggt aattaaagaa tttaaaaagc agcgtttccg gagaggaggt
     6601 cattttttcg agcactcccc ccgcaacccc gtcattcagc caaatcgccg tattgccgaa
     6661 gacggtaaac cacaaaacgc cgaacaggcc ggggatgagc aaaaccccga agacaaactc
     6721 gcggatggtg cgcccctttg aaatgcgcgc gataaacaaa cccacaaacg gcgcccaaga
     6781 acaccaccac gcccaataaa gcaccgtcca agattcaaac cacggcttgt gttcccgttc
     6841 gtacgcataa gttttaaaac tgaggcgcac cagatttccg aggtagttcc ctatgttgtc
     6901 gccgaatgcc gacaacaggt aaacagtggg acccgccgcc aaaacaaaaa acagcagcaa
     6961 aaacgcaagg ctcaggttca actcgctcaa caccttcacg cccttcccca cgccggatat
     7021 tgccgaaacg acggcgaggg acatgacggc ggcgataatc aaaacctgca cgctgaagct
     7081 gttttcggca atccagccca tttcctgcaa tccggcgccc agttgcgaag ccccgaaccc
     7141 caatgtggtg atgatgccga aaaaagtagc aagcaacgcc ataatatcaa tggcatcgcc
     7201 gaaccttccg gaaatttttt ctttcaacag ggggtaaaaa caagaacgca gggcaagcgg
     7261 cagcttgtag cggaaaccga aataagccaa agccaatgca atcgtaccgt acaccgacca
     7321 agcgtgaacg ccccaatgga acaccgtgtg cagcaatgcc tgctgctgcc tgtgttccgg
     7381 cgtgccggcc gtaatgtccg aaaaataatg catcaacggc tctgccacgc cgaaaaacat
     7441 cagacccacg cccatcccgg ccgcaaacag catcgccagc cacgacagga agccgaattc
     7501 cggcacatct tcatcccgtc cgagcctgat gtttcccaaa ctgctgaccg agagtatcag
     7561 caggaaaccc agaaaaatgg aaaacgttaa aacataaaac cagctgaact cggtaaaaat
     7621 gacttctttt gcccgatcga gccacatctg cgcctgatcc ggcacggtta aaaccaatac
     7681 caccaaaaca cacacaaaaa acaaagtcgt caaaataacc atcggattaa atgacgttcg
     7741 gcgttctata aattcagaca gggacaaacc ttctcactcc tttgttaaaa acagacaaac
     7801 ccggtcatcg ggcaaagcgg tcaaaacctg ccggtaaaat accggcttcc ggatgacgaa
     7861 atgcacaaac cggccgaaat tgttataatc gaaaaaatta aaaatcaata cggattattc
     7921 tatcagattg atttatcgat atttattata tcattaatat agtttgattt caaaccggcg
     7981 gaaaatgcgc cgcccaccgc aggcgcgccc tccccaccat cggggcgttt aacgccggat
     8041 tgccgatgcg gtacaatggc tgatatgaag aaaattaccc ctcaaaacct acgccccctg
     8101 ctttcggaaa gcttgggaca taccgatttt gtcaacgtcc tcaacgcact gattaaattt
     8161 ttgcgccgtg gcggcaaaaa atgtgcaggg gaacgtttcg acctgattat cgacacattc
     8221 aaacaagaca gggaattact gtcccgcttc agccggtgtt tttacatctg gctcgcgcaa
     8281 atacacattt atccggcact catcaaactc ggcatcttct cgcgccacag ctttgcccgg
     8341 gaaatgggca tacgcatcta cgaacgcttc agcccgtcat ataaagattt tgccaacttg
     8401 ggcgaagtct tcctttatct tttccattcc gaaaacgacg acaaatggct gcaaacgctc
     8461 aatatccgcc aatggctggt tttatacgaa ctcatccgga gccacgccga gccgtccaaa
     8521 ttgcagacgg cgggcatccg ccttgccgat gcgcgtttgc gcgccatcga aatgctgtct
     8581 gtctggacgg catccgaagc catcgaaccc gacctcatcc gcatcgcccc gcgcctgctg
     8641 gaagccgatt cttctttcgt cgccctccaa cgcgaaaccg ccaaactggt cgaacactac
     8701 cgcaacggca ccacgcctta cgacaccgcc cacctcgaag tgatgttcga ccaatgtttc
     8761 agccagattg actatttgcg ccgcaaaggg acgggcgccg gctctggttc gtcggtcaaa
     8821 gtcgcccacc tgctcgaacg gctccggcag accgtagacc gtctgaagct gctcaccgac
     8881 atccaaaccg gtgccggcaa cagcaaccgc ctgaccatcg cgctgatgaa ctccctcatc
     8941 tacgcggcgg tcgaacaata cagcacccgc cacctgcgcc gcagcagcat ccgtatgctc
     9001 gcccgcagca ttaccgaaaa caaaagccac cacggcgaac actacatcac ccgcaaccgc
     9061 aaagaatatt tcaaaatgtt ctactcggcg gcaggcggcg gcgtcatcat cgccctgatg
     9121 gcgctgctca aaatccgcat cggcacactc ggcctcagcc ccttcctcac ttccttgtcg
     9181 gcggggttca actacggcat cggctttatg atcatccata ttctgcactg caccgtcgcc
     9241 accaaacagc ccgcgatgac tgccgccagc ttcgccgaac aagttgatct caacgaaggc
     9301 ggcaaagcgg tggacaacaa gctcgccaag ctcctcatcg acgtatgccg ctcccaaagc
     9361 gtcgccgttt tcggcaacgt ttccatcgcc atccttttgg cgtgcgccat atcgttcggc
     9421 tatgcccatc tgcaccaaca gcccatactc gatgcccaca ccgccgccta ccagttcaaa
     9481 tccatagaca tcatcgctta cccgacgctg tggtatgccg ccattgcagg tctgtggctg
     9541 ttctgctccg gcatcatcgc aggttttttc gacaaccgcg ccgactacct caacctgcgc
     9601 caacgcctgc ccttcaaccc cttgctgcgt aaaatcatgc gccccgggcc ccgccgcgtc
     9661 ctcgccgcct acatccacaa acactacggc tcgttggcag gcaacttcat cttcgggatg
     9721 ctcttgggta tgaccggcta tttcggacac ctcctcgggc tgccgctgga cattcgccac
     9781 gtcgccttct cctccgccaa cctcggctat gctgccgtca gcggcaacgt cggtttgggc
     9841 acgttcgtac tcggcatttt cagcgtcctc gccatcggct tggtcaacct ctgcgtcagc
     9901 ttcagcctcg ccctcttcgt cgccctgcgc tcgcgcggca cgaaaatcgg cagtatccgc
     9961 aatctgatta agagtttttg gaatcagatt aaaagcaatc cctgcatact tttcctcccg
    10021 cccgccaaag aacagggaca tcctccttcg gacaagcctt aaccggcaat gccgtctgaa
    10081 gcgggattcg ccccgaatac cgccctgatg cggaaaatcc ccataaaggg atgcaaaaat
    10141 gccgtccgaa ccgcgtttcg gttcagacgg cattttatag tggattaaca aaaatcagga
    10201 caaggcgacg aagccgtaga cagtacaaat agtacggcaa ggcgaggcaa cgccgtactg
    10261 gttcaaattt aatccactat aaaaaaacat tacaatcccg ggccgccgag cgaattggca
    10321 atgtccctga ccgccgttac atacatccga ctgtgattgt actgccatac cgtataaaaa
    10381 ttgttcaagc ccaaataata ttcaaacacg ccgggtgcgg tttccagttt gaacaaaacc
    10441 gccttttcat catcggcaag ctcttcgccg gggatgatgc cgtacgcctt caaatccgcc
    10501 accgtccgcg tcagggcggt tttttcgcca atgattgcct gaacatccgc acccggcgcc
    10561 aatgttgcag acaccagtat tttcccgccc gtgcgccaac cgtgctgctt catataattg
    10621 gcaaccgatg ccgcgacatc gccgatgttg ccccatatgt cccgatgtcc gtctccgtca
    10681 taatccaccg cccatttccg gtagctcgaa ggcataaatt gcggcatccc cattgcgccc
    10741 gcatagctgc ctttaaaggc gaaaacatcg ccgccttctt cttttgccag ctttaaaagc
    10801 tcgaccaatt ctttttggaa aaacccggcg cggcgggggt aatcaaagcc taaggtcgcc
    10861 aatgcgtccg ccacacggaa actgcccgta tttttgccgt aattcgtttc aatcccgata
    10921 accgccacga taagttcggc aggcacgccg tatttttgcg ccacatcatc gataagcgcg
    10981 cggttttccg cataaaaccg gcgcgcgccg cgaaatttcg ccttgcccga atttcccgtg
    11041 cggaacacat accacggacg cgatgtggag ggtcggtgca taatcttgac gatgtccgcc
    11101 ttgtaagccg ctttgtcaaa aaaatcctgc cattccgccc gggaaaaatc ccctttcccg
    11161 acttcatcgt ccacaaaacg gcggacattt gcattggcgg caaacccgct gtcggatacc
    11221 ggtacggctg ccgcgtcaaa cgcggggcgg ctttcttttt tcatttcatc cgcgcggggg
    11281 gcttgggctt catttacccg gggcgggcgt gcctccatcg ccgtacaggc agacaaagcc
    11341 gccaaacaaa ttgccagcgg cagtattttt ctctttttca taaatatgtt ccgaacaaat
    11401 agggtaagtg ggaaagcggc gcaaggggcg cggtgcgaaa tgcggcatcc gccgcaatcg
    11461 gcggctttgt ggaataccgc gcgttgcctt ttgcaccgcc tgaaatccgt atgccgccgt
    11521 atttgcccgc tatccgcaat atcggcagtc caatatatct ttgcggatgt cgttcagcag
    11581 gaaggctgcg gtgtcttcgt gttccgcctg tacgaagaca aagagtcggg cgatgatttt
    11641 gccgatgaag actttttgca gggcgcaggc gtcggtcagg gtgtgttcct gcaggaagct
    11701 gcggatgtct tcgggcaaag tgtagtcgcg ggcgacggcg gcaaagcggg catcggtttg
    11761 caggcggtcg agcagggttt ggtttttgtc caacttcatg cctgcttctt tttcttcggc
    11821 ggtggcgcgg acgaactggt cgtaaatttc ggcataaagc atatcgttcg ggccttcaaa
    11881 aatcgtgaag gggcggatgt cgatagcgat attgccggcg gtgtgtccgc gttcaaaacc
    11941 cttcgcgccc aaaagttttt gcaacatttg cgcggcggcg taagtgtatt ccgtggcgag
    12001 ggttttgacg atgttcgcct ccatcagctg atgggcgacg ggtgcaacgg gcgaaacgga
    12061 atggcagacg tagcggtaaa gaatctcgga aacctgatgg cggcgccgga tttcgcggcg
    12121 ttcgtaatcg acgaatctga tgtcgttgcg gacgtatcgt tccaggtttt caaggatgta
    12181 ttccataatg ccgtgcgtca tgccgatcag ttgcaggcgg ctgcggataa agatgttttg
    12241 gaacgcgcgc aaaccggcag cgtcgctctg ggagagtttc atcacggcgg ttgcaggcat
    12301 ttcggcatcg atgcggttga cggcgtaacg gacggcgcgc aagccttcgg atgcgagggt
    12361 ttcgcagcgg atgtatgttt tggggacgag cagcaggtcg atgactttgg cgagtttgcc
    12421 gtttttgcgt tctttggcgg caacgaggag gaagtcgctt tgcgagttgc cctgccagta
    12481 ttttgcggcg ttgacgtaaa tggtttgtcc gtcggtatat tcgtagtaag actgcatttc
    12541 tcgggcaatc gccgcgccgg aggtttcggg ttcggtaacg cccaaaccac cgccctcgcc
    12601 tttgaaaacc atgtccaaac cctgtgcgat ttgcgcttca tcgccgaact cttgcagtgg
    12661 ctgcaacacc agcgcgcctt cgatgccggt acgcagcgta acgggcacgc cgtaatgccc
    12721 cgcaatccgc aagacttcct gaatttcaaa ctggctgccc ttgcgcccgc cgtgtttttt
    12781 gtcgaggaag ggcaacagca aacccgcctg cttcaaggca agccatttgt cttcgggcag
    12841 gtatcgcatc aggtcgatac cgtctgaaaa aatgcggcgg aatgcggatt cgatgtgctt
    12901 taaaaaagca gccgtgtcca tagttgacgg ctgcgcgctc ggttcggtgt gtatcatcgg
    12961 cttcctctgt cggttcccat taatcggcgg ccggtcaaac cgcctgccac agtttagagt
    13021 tgattttcta aactttacca caaagtgcgc cgggcaacaa tccgccgacc tttcagacag
    13081 catcgcgccc cctcccgtgc taaaatgacc gtttgcatca ctgtccgccg attgccgcac
    13141 tatgacctac cccatcccca aaccccgtga aaaatcccgt tggtccaatc tttcgcaagg
    13201 ctcgctgccc ttggctttgg cgcgttatct gccgcataag cggctcaagg tcgtgctgac
    13261 ccaagatgcg gaacaggcgc tacgccttca gacggcatgg cggtttttcc gtccgcacga
    13321 cacggcggtg ttcctgccgg actgggaaac gctgccttac gagcgttttt cgccgcatca
    13381 ggatttggtg tcggaacggc tgtcggcgtt gtggcagatt aagagcggcg cggcggacgt
    13441 gttgttcgtg ccggttgcca cggcgatgca gaagctgccg cccgtgccgt ttctggcagg
    13501 gcgcacgttt tggctgaaaa cagggcagac tttggatata ggccgtctga aaaccgattt
    13561 ggtagatgcg ggctacaacc atgtttccca tgttgttgcg gcgggcgagt ttgccgtgcg
    13621 cggcggcatt gtcgatttgt tccccatggg cagcgaaatg ccctaccgca tcgatttgtt
    13681 tgacgatgaa atcgacagca tcaaaacctt tgatacggac acgcagcgca ccatctcccc
    13741 cgtttccgaa atccgcctgc tgtcggcgca cgaattcccc accgacagcg aggcgcaaaa
    13801 aatcttccgc agccgcttcc gcgaggaagt cgatggtaat ccgaacgatg cggctgtata
    13861 caaagcagta agcaacggcc acttcggtgc gggcgtggaa tattacctgc cgctgttttt
    13921 tgaaaacgag ctggaaacgc tgtttgacta tatcggcgaa gacgcgctgt ttgtctcttt
    13981 gggcgatgtt cacgccgagg caaaccgctt ttggagcgac gtcaaatcgc gttacgccat
    14041 ggcgcagggc gacgaaacct atccgccttt gcttccacag catttgtatc tctcctcaga
    14101 tgtttttgca ggccgtctga aaaattatgg tcaggtattg ccggatattt ttggtgttga
    14161 acatacgttg ccaaacgtgg ccgtcaaccg ccaagcggat gaaccgttgc aggcattgaa
    14221 agattttcag acggccttta aaggacggat tttgttgtgc gccgaaagtt tgggacgacg
    14281 cgaaaccatg ctcggtttct tgcagcaaaa cggtttgaaa gccaaacccg tgtccgactg
    14341 gcagggcttt ttatcggcac acgagccgct gatgattaca gtggcgccgt tggcatacgg
    14401 gttcaaactg ggcggactgc aatcgccgaa ccaacagcaa actacttccg cctccgaggg
    14461 agaaggcgat gcggttaccg accaaaccga attttccgca gccgcaacaa accctctccc
    14521 tagccctctc ccacaggaga gggaacaaag
//
LOCUS       DAWFIE010000050        14541 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWFIE010000050 DAWFIE010000000
VERSION     DAWFIE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.050"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            39..893
                     /locus_tag="WGQ71_001371"
     CDS             39..893
                     /locus_tag="WGQ71_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0707372.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGVGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
     gene            1413..2645
                     /locus_tag="WGQ71_001372"
     CDS             1413..2645
                     /locus_tag="WGQ71_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ0707373.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLG
                     ADVHRIEQSLSVFMFGTAFGQVVGGSVSDIKGRKPVALAGLTVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMAGAVL
                     QSLGGWQAIFVFLAAYSLVLLGLVRHFLPKPTAGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
     gene            2684..3811
                     /gene="pheA"
                     /locus_tag="WGQ71_001373"
     CDS             2684..3811
                     /gene="pheA"
                     /locus_tag="WGQ71_001373"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ0707374.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASSSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            3845..4636
                     /gene="recO"
                     /locus_tag="WGQ71_001374"
     CDS             3845..4636
                     /gene="recO"
                     /locus_tag="WGQ71_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ0707375.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWVGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADSTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLLIGTLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            4660..5388
                     /gene="pdxJ"
                     /locus_tag="WGQ71_001375"
     CDS             4660..5388
                     /gene="pdxJ"
                     /locus_tag="WGQ71_001375"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ0707376.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVIELHTGAYADAH
                     SHAEQMKQFERIQNGAHYASDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            5434..5541
                     /locus_tag="WGQ71_001376"
                     /pseudo
     CDS             5434..5541
                     /locus_tag="WGQ71_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            5540..6319
                     /locus_tag="WGQ71_001377"
     CDS             5540..6319
                     /locus_tag="WGQ71_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ0707377.1"
                     /translation="MNQKYILSANNNSLIEEIHNTVQSIGYCIVRGLNLNHLDDSRRN
                     KKLFDFLSQLGMLTNHKDDGFKSIFWDIKYRGDDYVINNDITFSEDVGECPLHSDSSF
                     SENPESYLVMYVVKSANDGGNSLFLSSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPT
                     SFDKKQGVRWGNILSVNTQMIRFRRDCIYKGIEENRNKVSKEMVLALDYLVNVIKNAS
                     DIQEFSAQDDDLIIIDNVNGLHARTDYTDKNRHYIRARITV"
     gene            6329..7096
                     /locus_tag="WGQ71_001378"
     CDS             6329..7096
                     /locus_tag="WGQ71_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0707378.1"
                     /translation="MQEIIQSIVFIAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIAKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            7296..7673
                     /locus_tag="WGQ71_001379"
     CDS             7296..7673
                     /locus_tag="WGQ71_001379"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ0707379.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAVRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            7755..8564
                     /locus_tag="WGQ71_001380"
     CDS             7755..8564
                     /locus_tag="WGQ71_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0707380.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQAWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEESGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADVVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            8557..8979
                     /locus_tag="WGQ71_001381"
     CDS             8557..8979
                     /locus_tag="WGQ71_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707381.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPVGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            9028..10170
                     /locus_tag="WGQ71_001382"
     CDS             9028..10170
                     /locus_tag="WGQ71_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ0707382.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELGSLGCTDVQAFDGGVSCRGGL
                     EQAYAANLHSRTASRILLHLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLNERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLLAGYDGTQPFQDPFCGSGTIAVEAAWIATGRA
                     PGMMRRFGFEKLQNFDKTLWTDLRRRAEAQIRPVRAPIAGSDSDRRIVQTALDNARSA
                     EVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTSDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(10471..11289)
                     /locus_tag="WGQ71_001383"
     CDS             complement(10471..11289)
                     /locus_tag="WGQ71_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0707383.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTGLSDRLPLSVLTGSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FAPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
     gene            complement(11356..12606)
                     /locus_tag="WGQ71_001384"
     CDS             complement(11356..12606)
                     /locus_tag="WGQ71_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0707384.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(12603..13064)
                     /gene="tsaE"
                     /locus_tag="WGQ71_001385"
     CDS             complement(12603..13064)
                     /gene="tsaE"
                     /locus_tag="WGQ71_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ0707385.1"
                     /translation="MPDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLEPFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLTAHTERGRESLPL"
     gene            13254..14066
                     /locus_tag="WGQ71_001386"
     CDS             13254..14066
                     /locus_tag="WGQ71_001386"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ0707386.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT         3331 a         3698 c         4033 g         3479 t
ORIGIN      
        1 ctatactttt gtatgaccat ctgactttat cactcactat gttttaccaa atccttgccc
       61 tgattatctg gagcagctcg tttattgccg ccaaatatgt ctatggcggc atcgatcccg
      121 cattgatggt cggcgtgcgc ctgctgattg ctgcgctgcc tgcactgccc gcctgccgcc
      181 gtcatgtcgg caagattccg cgtgaggaat ggaagccgtt gctgattgtg tcgttcgtca
      241 actatgtgct gaccctgctg cttcagtttg tcgggttgaa atacacttcc gccgccagcg
      301 catcggtcat tgtcggactc gagccactgc tgatggtgtt tgtcggacac tttttcttca
      361 acgacaaagc gcgtgcctac cactggatat gcggcgcggc ggcatttgcc ggtgtcgcgc
      421 tgctgatggc gggcggtgcg gaagagggcg gcgaagtcgg ctggttcggc tgcctgctgg
      481 tgttgttggc gggcgctggc ttttgtgccg ctatgcgtcc gacgcaaagg ctgattgcac
      541 gcatcggcgc accggcattc acatctgttt ccattgccgc cgcatcgttg atgtgcctgc
      601 cgttttcgct tgctttggcg caaagttata ccgtggactg gagcgtcgga atggtattgt
      661 cgctgctgta tttgggcgtg gggtgcggct ggtacgccta ttggctgtgg aacaagggga
      721 tgagccgtgt tcctgccaac gtgtcgggac tgttgatttc gctcgaaccc gtcgtcggcg
      781 tgctgctggc ggttttgatt ttgggcgaac acctgtcgcc cgtgtccgcc ttgggcgtgt
      841 ttgtcgtcat cgccgccacc ttggttgccg gccggctgtc gcatcaaaaa taaagttggg
      901 aagcggtatt tgatgattgc cgaataggct gaaatctttc catctccatt cctgcgaaag
      961 cgggtatccg gaacgaaaag acggatattt atccgaaata acgaccatct ttgcatcgtc
     1021 attcccgcgc aggcgggcat ccggtttttt gagtttcggt tatttccgac aaattgctgc
     1081 agcgttggat gtccggattc ccgcctgcgc gggaatgacg ggattttata gcggattaac
     1141 aaaaatcagg acaagacgac gaagccgcag acagtacaga tagtacggaa ccgattcact
     1201 gggtgcttaa gcaccttaga gaatcgttct ctttgagcta aggcaaggca acgctgtact
     1261 ggtttttgtt aatccactat atcgttccgg ttcgtccggt tttgccgggg cttttgttgc
     1321 cgcctgtttg tgccggtgtg ttaaaatttt ccgtttccgc gtattgtgtt ttccgccgcc
     1381 gggcggtttg tttgcgaatc ggacgagaat ttatgccttc tgcccattat cctgaaatga
     1441 gcgaaaaact gatggcggtt ttgatggcga tgctggttac gctgatgccg ttttccatcg
     1501 atgcctacct gcccgcgatt cccgaaatgg cgcaatcgct gggcgcggat gtccaccgta
     1561 tcgaacagag cctgagtgtg tttatgttcg gtacggcgtt cggacaggta gtcggcggtt
     1621 cggtgtccga catcaagggg cgcaaacccg tcgcgctggc gggtctgacc gtttactgcc
     1681 ttgccgttgc cgccatcgta tttgcttcga gtgccgaaca gcttctcaat ctgcgcgtcg
     1741 tgcaggcatt cggcgcgggc atgactgtgg tcatcgtcgg cgcgatggtg cgcgattatt
     1801 attcggggcg gaaagccgca cagatgtttg cccttatcgg catcattctg atggttgtgc
     1861 cgctggttgc acctatggcc ggcgcggtat tgcagagttt gggcggctgg caggcgattt
     1921 ttgtttttct ggcggcgtat tcgctggtgc tgctcggttt ggtgcggcat ttcctgccca
     1981 aacctaccgc cggcggcaaa atcggacggg acgtgttcgg gctggtggcg gggcggttca
     2041 agcgcgtatt gaaaacccgt gccgcgatgg gttatctgtt ttttcaggca ttcagcttcg
     2101 gttcgatgtt cgcctttctg accgaatctt ccttcgtgta ccagcagctt taccgtgtta
     2161 cgcctcatca atacgcttgg gcgtttgcac tcaacatcat cacgatgatg tttttcaacc
     2221 gcgttaccgc gtggcggctc aaaaccggcg tgcatccgca aagcatcctg ctatggggga
     2281 ttgtcgtcca atttgccgcc aacctgtccc aactcgccgc cgtgctgttt ttcgggttgc
     2341 ccccgttttg gctgctggtt gcgtgcgtga tgttttccgt cggcacgcag ggcttggtcg
     2401 gcgcgaacac gcaggcatgc tttatgtctt atttcaaaga agagggcggc agcgcgaacg
     2461 ccgtgttggg cgtgttccag tccttaatcg gcgcgggcgt gggtatggcg gcgaccttct
     2521 tgcacgacgg ttcggcaacc gtgatggcgg caacgatgac cgcgtccacc tcttgcggca
     2581 ttgcgcttct gtggctgtgt tcgcaccggg cgtggaagga aaacgaacag agcgaatacc
     2641 tttaacggaa aatgccgtct gaaaccgttt cagacggcat ttgatgttag aatgcacgat
     2701 aaattactgt tcaggcgaaa ttatgtccca aactatcgac gaactcctcc ttccccaccg
     2761 caacgccatc gacaccatcg atgccgaaat cctgcgcctg ctcaacgaac gcgcgcaaca
     2821 cgcccacgcc atcggcgaac tgaaaggcac gggcgcagta taccgtccgg agcgcgaggt
     2881 cgccgtgttg cgccgcattc aggatttgaa caaaggcccg ctgcccgacg aatcggtagc
     2941 acgcctgttt cgggaagtga tgagcgagtg tctcgccgtc gaacgcccgc tgaccatcgc
     3001 ctatctgggg ccgcagggca cgtttaccca gcaggcggcg attaagcatt tcggacacgc
     3061 cgcgcacacc atggcgtgtc cgaccataga caactgcttc aagcaggttg aaacccgtca
     3121 ggcagactat ctggtcgccc ccgtggaaaa ctcgaccgaa ggctcggtcg ggcgcacgtt
     3181 ggatttgctt gccgttaccg cgttgcaggc gtgcggcgaa gtcgttttgc gcatccacca
     3241 caaccttttg cgtaaaaaca acggcagcac cgaaggcatt gccaaagtct tttcccacgc
     3301 gcaggcgttg gcgcagtgca acgactggtt gggcagacac ctgcccaacg ccgaacggat
     3361 tgctgtgtcc agcaatgccg aagccgcaag gctggttgcc gaatcggacg acggtacggt
     3421 tgccgccatc gccggacgca cggcggcgga aatctacgga ctcgatatgg ttgccgagtg
     3481 catcgaagac gaaccgaaca acaccacgcg cttcttggtg atgggacatc acgaaaccgg
     3541 tgcaagcagc agcgacaaga cttcgctggc cgtttccgcg cccaaccgcg ccggtgcggt
     3601 tgcctcgctg ctgcaaccgc tgaccgagtc gggtatttcc atgaccaagt ttgagagccg
     3661 tccgagcaaa tccgttttgt gggaatacct gttcttcatc gacatcgaag gacaccgccg
     3721 ggatgcacag attcagacgg cattggaacg cttgggcgaa cgcgcttcgt tcgtcaaagt
     3781 catcggttcg tacccgaccg ccgttttgta gcggcggcag cgttcagacg gcatttcagg
     3841 catgatgtcc gaacaccgag tcaaccacga acccgtcttc ctgttggctt cctcgccatg
     3901 gcgcgaaagc agcctgcggg tcgaagcctt cagccgccgt tacgggcgtg tggcactttt
     3961 ggcaagaagc gcgcgcaagc ggcagagcga attgcgcggc gtattggttc ctttcgtgcc
     4021 cgtcagcgcg tcgtggtacg gcagtcagga actcaaaacc ctgcaccgtg ccgaatgggt
     4081 cggcggatgg cggcagccgc agggcagggc gttgttcagc ggtctgtatg tgaacgaact
     4141 ggtgttgaaa ctgaccgcgc gcgaagatcc gatgcccgaa ctgtacgacg cgttggcaaa
     4201 agtgatggag gcggtgtgcc gtgaagccaa ccatattgcc gatttgcgcc gcttcgagtg
     4261 gaagctgctc aatgccttgg gcgttgcccc cgatttgcac gccgacagca cgggcggcga
     4321 tattttggcg gacaaaacct accggctgat gcccgaagag gcggtcatgc ccgtatgcga
     4381 ggacgcagac gcgctttcgc acgaagccgg cgcaattgtc gaagggcaaa gcctgatcga
     4441 tttgcgcgaa ggcagtttcc gcaccgccga aagcctgcaa caggcattga aaatcacacg
     4501 gctgctgatc ggcacgctgc ttcccgaagg cttgaaatcg cggcaggtgt tggaacagat
     4561 acggcagttt gaccgcaaag aaaccgcccg ggaaaccgtc ccgacttcgg acggcacggc
     4621 ttcaaatgcc gtctgaaggc agaaataaaa ggaaagatta tgcttttagg tgtcaacatc
     4681 gaccacatcg ctaccgtccg caatgcgcgc ggcacgactt atcccagccc cgtggaggcg
     4741 gcactggttg ccgaaacgca cggtgcggat ttgattacca tgcacctgcg cgaagaccgc
     4801 cgccacatca aagacgcgga tgtgtttgcc gtcaaaaacg ccatccgcac gcgcctgaac
     4861 cttgaaatgg cgttgacgga agaaatgctc gaaaacgcgc ttaaagtgat gccggaagac
     4921 gtgtgcatcg tgcctgaaaa acgtcaggaa atcacgaccg aaggcggctt ggacgtattg
     4981 gcgcaacagg aaaaaatcgc cgggttcacc aaaatcctga ccgacgcagg catccgagtc
     5041 tctctcttta tcgatgccga cgacaggcaa atccaagccg cctatgatgt cggcgcgccc
     5101 gttatcgaat tgcacaccgg cgcatatgcc gatgcacaca gccacgccga gcaaatgaaa
     5161 caattcgagc gcattcaaaa cggtgcgcat tacgccagcg atttgggttt ggtcgttaac
     5221 gccggacacg gcctgaccat tcacaacgtt acgccgattg cccaaatcct cgccatccgc
     5281 gaactgaaca tcgggcattc gctgattgcc caagccctct tcctcggact gcccgaagcc
     5341 gtgcgccaaa tgaaggaggc gatgttcagg gcaaggctgc tgccgtaagg gcaggcaaac
     5401 cctttcagac agcatttcac gacagggata tgttatagtg gattaaattt aaatcaggac
     5461 aaggcggcga agccgcagac agtacaaata gtacggcaag gcaagccaac gccgtactgg
     5521 tttaaattta attcactata tgaatcaaaa gtatatttta tctgcaaaca ataatagttt
     5581 gatagaagaa attcacaata cagtacagag tattgggtat tgtattgttc gcggtcttaa
     5641 tctaaaccat cttgatgaca gccggagaaa caagaaatta tttgactttc tatctcaatt
     5701 aggaatgctg acaaaccaca aagacgatgg ttttaaatct atattttggg atattaaata
     5761 tcgcggcgat gactatgtaa taaataatga tataactttc tcggaagatg ttggagaatg
     5821 tccacttcat agtgattcat cttttagtga aaacccggaa agttatttgg ttatgtatgt
     5881 agtaaaatca gccaatgatg gaggtaattc cctattttta agttcatcag atattgtcaa
     5941 tcagttatct aaaacagaaa ctggtaaaaa acacttaaaa acattaacgg gcaatttata
     6001 tccatttaaa acaccaacat catttgataa aaaacaaggt gtgagatggg gtaatatctt
     6061 atcggtcaat actcaaatga ttagatttag aagggattgt atctataaag gtattgaaga
     6121 aaatagaaat aaagtatcaa aggaaatggt acttgcactt gattatcttg taaatgttat
     6181 aaaaaatgcg agtgatattc aagaattttc tgcacaagat gatgatttga ttattattga
     6241 caatgtcaat ggtttgcatg ccagaactga ttatacggat aaaaacaggc attatattag
     6301 agcaagaatt actgtataaa ggacggttat gcaagaaata atacaatcta ttgtttttat
     6361 tgctgccgca atactgcacg gaattacagg catgggattt ccgatgctcg gtacaaccgc
     6421 attggctttt atcatgccat tgtctaaggt tgttgccttg gtggcattac caagcctgtt
     6481 aatgagcttg ttggttctat gcagcaataa caaaaagggt ttttggcaag agattgttta
     6541 ttatttaaaa acctataaat tgcttgctat cggcagcgtc gttggcagca ttttgggggt
     6601 gaagttgctt ttgatacttc cagtgtcttg gctgctttta ctgatggcaa tcattacatt
     6661 gtattattct gttaatggta ttttaaatgt atgtgcaaaa gcaaaaaata ttcaagtagt
     6721 tgccaataat aagaatatgg ttctttttgg gtttttggca ggcatcatcg gcggttcaac
     6781 caatgccatg tctcccatat tgttaatatt tttgcttagc gaaacagaga ataaaaatcg
     6841 tatcgcaaaa tcaagcaatc tatgctatct tttggcgaaa attgttcaaa tatatatgct
     6901 aagagaccag tattggttat taaataagag tgaatacggt ttaatatttt tactgtccgt
     6961 attgtctgtt attggattgt atgttggaat tcggttaagg actaagatta gcccaaattt
     7021 ttttaaaatg ttaattttta ttgttttatt ggtattggct ttgaaaatcg ggcattcggg
     7081 tttaatcaaa ctttaattca ttattaaatg ccttaactcc ttattaaata attggcacga
     7141 tgttttagaa tttcaaatgc aaaaggttac aataaaaatt gttaccgaca aaaccccgaa
     7201 agtggatatt cacgccattt taacgcccca agaaattgac ggcattcatc atcacattca
     7261 tcactacccg caaccaaggg cgaaggagcg caaatatgat ttacggcatc ggtacagaca
     7321 tcgtttccct caagcgcatc atccgcttaa acaaaaaatt cggacaggcg tttgccgtgc
     7381 gtatcctcac tccggaagag ctgcttgaat ttcctcaggc gggcaaaccc gtcaactacc
     7441 ttgccaaacg ctttgccgcc aaagaagcct ttgccaaagc cgtcggcacg ggcatacgcg
     7501 gcgcggtttc cttccgcaac atcggcatcg ggcatgacgc attgggcaag cccgaatttt
     7561 tctacggccc cgccctgtcc aaatggctgg aggaacaagg catcagccgc gtcagcctca
     7621 gcatgagcga cgaagaagac accgtattgg cgtttgtcgt tgccgaaaaa taatgccgtc
     7681 tgaaatgcgg caaacccgtt gacggcattg cccgtccctc atttgcactc cgaccgacca
     7741 accgcgtacc cgccatgatt caagacaccc gaccccttat ccgcgtcgtt gccggcatcc
     7801 tgctcgattc agacggcaac tacctgctca gctcgcgccc cgaaggcaaa ccctatgccg
     7861 gatattggga atttgccggc ggcaaggtcg aagcgggcga aaccgacttc caagccttgc
     7921 aacgcgagtt tgaagaagaa ctcggcatcc gcatcctcgc cgccacgcct tggctgacca
     7981 aaatccattc ctacgaacac gctcgcgtct gcctgaaatt cctatgggta aaccccggcc
     8041 aatggacggg cgaaccgcaa tcgcgcgaag gacaggcatg gtcgtggcag aaggcgggtg
     8101 attttaccgt tgcccccatg ctgcctgcca acggcgcgct tttgcgttcg ctgtccgtcc
     8161 cgcgccgttt gtacggcagc ctgaaaacgg gtttgcacgg ggagaacagt atgggcgcgt
     8221 accgcgtcct gcctttgggt tcggcagagg aaagcggtgc gaacgttttg atggaggcgg
     8281 cgcaatggca ggacagaccc gaacacgccg acagcgtgtg gatgatggtg cagacccgcg
     8341 aacaatggcg gcaggcgcag gaaaagggcg cggatgtggt cgtctggcgc gtgtgcgatg
     8401 atgttcaggc acaagaggcg gcagaagccc tgcggcaggg cgtatctgtg ccgctcgtac
     8461 ttgcagcaaa cggacagacg gttgcacgtt atggaaaact atggctcgga ttgggggcgc
     8521 acgtggtggt aagggatgaa acaataggga agaatcatga ataaaaaccg taaattactg
     8581 cttgccgcac tgctgctgat tgcctttgcc gccgtcaagc tcgttttgtt gcaatggtgg
     8641 caggcgcagc agccgcaagc cgtggcggcg caatgcgatt tgaccgaggg ttgcacgctg
     8701 ccggacggaa gccgcgtccg cgccgccgcc gtttcaacca aaaaaccgtt tgatatttat
     8761 atcgaacacg cgcccgtcgg cacggaacag gtcagcatca gcttcagtat gaaaaatatg
     8821 gatatgggtt tcaaccgcta tatgttcgag cggcaaccgt cggggacttg gcaggcagta
     8881 cgcatccgcc tgcccatctg tgtcgaaggc aggcgcgatt ttacggcgga cattacaatc
     8941 ggcagccgga catttcagac ggcatttacc gccgaataaa cctttcaatc cgccattgcc
     9001 ggaacatccg tccggaaagg acacgttatg aatactttat atacactttt cgccacctgc
     9061 ccgcgcggct tggaaaccgt tttatctcaa gaactcggaa gcctcggctg taccgatgtt
     9121 caagcgtttg acggcggcgt ttcctgccgc ggcggattgg aacaggccta cgccgccaac
     9181 ctgcactcgc gcaccgccag ccgtatcctg ctgcacctga ccaaaggcac ataccgaacc
     9241 gagcgcgaca tttacaaact tgccaaaaac atcaactggt tcaactggtt taccttgcag
     9301 cagacgttca aagtcaaagt cgaggcaaag cgtgccaacg ttaagagcat ccaatttgtc
     9361 ggactgaccg tcaaagatgc cgtctgcgac gctttccgcg acatttacga cgcacgtccg
     9421 agcgtggaca aagccgcgcc cgatgtccgc atccacgcct ttttgaacga acgcaatgtc
     9481 gaaatcttta tcgacacttc gggcgaagcc ctgtttaaac gcggctaccg cctggatacc
     9541 ggcgaagccc cgctgcgcga aaaccttgct gccggactgc tgctcttggc aggctacgac
     9601 ggcacgcagc cgtttcaaga cccgttttgc ggcagcggca cgattgccgt cgaggccgct
     9661 tggattgcca caggccgcgc gccgggtatg atgcgccgtt tcggttttga gaaactgcaa
     9721 aatttcgata aaacgctgtg gacagatttg cgccgccgcg ccgaagcgca aatccgcccc
     9781 gtccgcgccc cgattgcagg cagcgacagc gaccgccgca tcgttcagac ggcattggac
     9841 aacgcacgca gtgccgaagt ggacgacatc gtttccttca gcgttgccga cgcgcaatcc
     9901 gtccgtccga acggcgaaaa cggcatcatg gtgtccaatc cgccctacgg tgtgcgcctt
     9961 gaggaagtcc gcgctttgca ggctctttat ccgcagctgg ggacgtggtt gaaaaaacac
    10021 tatgccggct ggcgggtcgg catgtttacc agcgacaggg aaatgcccaa attcatgcgc
    10081 ctgtcgccca agcggaaaat tccgctttat aacggcaacc tcgactgccg cctgttcctg
    10141 attgatatgg tgcagggatc gaaccgttga ggaaagtgta caaaaaagcc gtctgaaaaa
    10201 tgttcagacg gcttttttta atatccggcc gatattatag tggattaaca aaaatcagga
    10261 caaggcgacg aagccgcaga cagtacaaat agtacggaat cgattcactt ggtgcttcag
    10321 caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg gtttttgtta
    10381 attcactata accgaaggta agggaggggt gtgtccactg tgccgccgca tttgccgcgt
    10441 gcggaagata cggataacaa ccccttggaa ttaaagggag aagttgcggg aaatatccat
    10501 atcaggctgg ggaccgtact tatttacggt attttcgtat gccgaaataa aatatttctt
    10561 gaataaaccg taatcgccgg tttcgtaata ggctacgaca gcctcggcat aggcgggata
    10621 actgtcgggg gcgaatacgc aaggaaaaaa accggagcgc atcagcgaca aggtcatgca
    10681 gtttctggca gtacgtttat tgcaatcttt aaaatattga agatacgcca aattattatg
    10741 gagataaacg gcgcggtcga aaggattttc tattttggga gcttcttgaa gcaaccattt
    10801 taattccgta tccaaacttt gcgggttaga taaaggggtg tagtctgtcc cgcttatggt
    10861 aacgctgtcg cggcggacta cgccgccgga gcctttttcc agtaagtttt cggaaatcag
    10921 gctgtgggtg gtttttaaga aatccaacca gtcaaacggc tcggggctgt ccaaaccgga
    10981 cagcaggtgc cggtaacttt ccctcaggtt gattaacatt acggcatccg aatataactt
    11041 cccgcctgcg gtttgcccga gtttaagcaa tgcctgcgta tcgtattgat tataagtgtt
    11101 tccttccaat ttggcagaac tatatacaaa atcgataccg atttcttcaa tattacgcac
    11161 ggagcccgtt aaaacagata aaggaagccg atcggacaat ccggtcaggt attcttgttc
    11221 gccggctgta aaaatggggc ggatatttaa aaaatcctgt tggtatctca atttatccat
    11281 acctttcatc ataaaaacgc ggtttggggc ggaagtaggt ttatgcctgc cttcatccga
    11341 ctaccgcctt ttcaatcaac cccgcttcaa tacggatgta ttgatgtagc gttggacacc
    11401 cgaggcaatg gattgggcgc actgccggcg gaaggattcg ctgcccagca acttctcttc
    11461 agcagggttg gacaggaagg cggtttcgac caagatagac ggcatatcgg gcgcgcgcaa
    11521 aacggcgaaa ttggcttcgt ccaccctgcc tttgtgcaga tggttgagcc tgcccaattc
    11581 ttcaagcacc agtttgccga gtttgcggct gtcgcgcagc gtggcggttt gggtcatatc
    11641 gagcagggcg gtatcgacat tgcggttgcc gctggtcggt acgccgccga ccgcgtcggc
    11701 attgttttgg gtttgttcca agaatttggc ggcagagctg gtcgcgcctt tggtgttcaa
    11761 catataaacc cctgtgccgc gcgcggaggg gctggtgaaa gcgtcggcgt ggatggagac
    11821 gaatacgtcc gcccgccgtg cgcgcccttt ggcgacacgc acgcccaatg ggatgaacac
    11881 gtcttcgttg cgcgtcataa agacgttgta acccaaggct tccaattgtt ttttggtttc
    11941 gcgggcgatg gagaggacga cgtgtttttc ctgcaaaccg cccgggctga cggcgccggg
    12001 gtcttcgccg ccgtgtccgg ggtcgatcat aatgacgggt ctgcgcccgt ttttgccgcg
    12061 ttcggtttgt gcgggagtgt tttgagcgag gtcggcttca ggggagccgc gacgggtttt
    12121 gttcaggctg ccgttaagca gcgccatcat cggatcgtcg ggatccatcc cttgcggata
    12181 gagatcgacg acgaggcggt ttttaaagcc gccgacgggg gggagggaaa aaacttgcgc
    12241 gtaggaaggc tgtttcaaat cgatgacgag gcggacggtg gtcggcgtgt tctgacccgc
    12301 gcgtatgctg cggataaaag ggtcgtccgc cataaccttc tgagacagtc cgtgcaatac
    12361 ggtattgatg ttcgcgtttt gtatatcgac gaccagcctg cccgggttgt cgagcgtgaa
    12421 gtgctggtat ttgagcgcgg cggtgctttc cagcgttagg cgggtgtagg tgtgcgacgg
    12481 ccatatccgt gcggcggtaa actgcggggc gcgtaccgtt ttggcaacgg cggatgcgat
    12541 ggggcttagg gcgaacagtg tgccggcggt gcggcggatg atttgtcttc gtgtcagttt
    12601 ggtcatagcg gcaggctttc gcgtcctcgt tcggtatggg cggtcagcag gcattttctg
    12661 ccgccgccgt cgtgtgtcaa tgttgcggtg atgtcggcgg gcggggtaaa ttcgcctccc
    12721 tgttgcggcc attcgatcag gcagacgctg tttgcggcaa acagttcgtc aagccccgcg
    12781 tcttcccatt cttcggggaa cgagaagcgg tagaggtcga aatggtgcag ggtaaagggt
    12841 tccagcggat aagattcgac gatggcgtag gtcgggcttt tgactgcgcc ctgatgaccc
    12901 aatccgcgca ggatgccgcg tgtcagcgtg gttttgcccg cacccaaatc cccttcgaga
    12961 taaatgacca gcggtgcgtt taaacgggaa gaccacgccg cgcccaaatc gagtgtggcg
    13021 gcttcgtcgg caaggaatcg ggaaacgggg ggaaaatcag gcataggggc tttacaactt
    13081 ttatgtggcg gatcggaggg gcaggcggtc ggcaaaccgt gcgcccggcg gcagcattgc
    13141 cgtacattat aacagccaaa tccgcatcct gctttcagac ggcaacggct gtcaagaaaa
    13201 agcggcgcgt gtacaatacg cggattgtat gtttaggacg gattggaaaa aggatggaaa
    13261 atatcggcag gcagcgaccc atcggcgttt ttgactcggg aatcggcggt ttgaccaatg
    13321 tgcgcgcact gatggagcgg ctgccgatgg agaacatcat ttatttcggc gatacggcgc
    13381 gcgtgcctta cgggacgaaa tctaaggcga ccatcgaaaa tttctcgatg cagattgtcg
    13441 attttttatt ggaacacgat gtcaaggcga tggttatcgc gtgtaatacg attgcggcgg
    13501 tggcggggca gaaaatccgt caaaagacgg gcaatatgcc ggttttggac gtgatttccg
    13561 ccggcgcgaa agccgcgttg gcaacgacgc gcaacaacaa aatcggcatt atcgccacca
    13621 ataccacagt taacagcaat gcttatgcgc gcgccatcca tagggacaac cccgacacgc
    13681 ttgtccgcac gcaggccgcg ccgctgctcg tccctttggt ggaggaaggt tggttggaac
    13741 acgaagttac ccgcctgacc gtatgcgaat acctcaaacc attgcttgca gacggcatcg
    13801 atacgctggt gttgggctgc acgcactttc ccttgctcaa acccttaatc ggcagggagg
    13861 cgggcaatgt cgcgctggtc gattccgcga ttacgactgc cgaagaaacc gcacgcgtcc
    13921 ttgcgcagga aggattgctc aataccgaca acaacaatcc cgactaccgt ttttacgtca
    13981 gcgatattcc tttgaaattc agaaccatcg gcgagcgttt tctgggcagg acgatggagc
    14041 agattgaaat ggtgtctttg ggttaaaacg atgacggaaa gctgcccgag attacagaaa
    14101 cctaaaatcc cgtcattccc acgaaagtgg gaatctagac ctgtcggtgc ggaaacttat
    14161 cggataaaac ggtttcttta gattttacgt tctagattcc cgctttcgcg ggaatgacgg
    14221 gatgcaggtt tctgtgcaga tgtattcgga ttccctcctg agcgggaatg atgggatttt
    14281 taaatgtccg aatgccgcaa atgccgtctg aaaggcttca gacggcattt tttacacaat
    14341 tttcaccgtt ttccatcctt ctcgatacac tgtaatctcg aaacccgtca ttcccgcgca
    14401 ggcgggaatc cagaccgttc ggtttcggtt tttttgaggt ttcgggtaac ttctaaaccg
    14461 tcattcccgc gcaggcggga atccagaccg ttcggtttcg gtttttttga ggtttcgggt
    14521 aacttctaaa ccgtcattcc c
//
LOCUS       DAWFIE010000051        14507 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWFIE010000051 DAWFIE010000000
VERSION     DAWFIE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14507
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.051"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(119..760)
                     /locus_tag="WGQ71_001387"
     CDS             complement(119..760)
                     /locus_tag="WGQ71_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0707387.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSYDKLDVSDFIHYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTPSQQLKILRD"
     gene            complement(993..3050)
                     /gene="ppk1"
                     /locus_tag="WGQ71_001388"
     CDS             complement(993..3050)
                     /gene="ppk1"
                     /locus_tag="WGQ71_001388"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ0707388.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAQEGIHFYRRRNWTGAQKKWIESYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(3219..3662)
                     /locus_tag="WGQ71_001389"
     CDS             complement(3219..3662)
                     /locus_tag="WGQ71_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ0707389.1"
                     /translation="MPLRLLLGILCDLSMVAPVYAQGQPDAVNAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGTATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(3669..4184)
                     /locus_tag="WGQ71_001390"
     CDS             complement(3669..4184)
                     /locus_tag="WGQ71_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0707390.1"
                     /translation="MKIKQIVKPGLAVLAAGILSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDLMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GTDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            4457..7087
                     /locus_tag="WGQ71_001391"
     CDS             4457..7087
                     /locus_tag="WGQ71_001391"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ0707391.1"
                     /translation="MQEQYRPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKRLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDS
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYARFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFATKYSLPKKQVIAVGDNAFDANQWQEWYGDKENGV
                     LVNSGDLDGMNFQTAFDAIAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAEAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGNQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPAVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            7315..8106
                     /locus_tag="WGQ71_001392"
     CDS             7315..8106
                     /locus_tag="WGQ71_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0707392.1"
                     /translation="MNLFFDTELGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMKR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNIVPDGAYHTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNTIEFIGRGLYKKIDKLRPTSKAV"
     gene            8236..8367
                     /locus_tag="WGQ71_001393"
     CDS             8236..8367
                     /locus_tag="WGQ71_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ0707393.1"
                     /translation="MRNFDKLAEEAAEHAAEGAVKFIEGKPAKKIIAVPGRLANIVV"
     gene            8390..9318
                     /locus_tag="WGQ71_001394"
                     /pseudo
     CDS             8390..9318
                     /locus_tag="WGQ71_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
     gene            complement(9406..9606)
                     /locus_tag="WGQ71_001395"
     CDS             complement(9406..9606)
                     /locus_tag="WGQ71_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707394.1"
                     /translation="MPDRIKPSRNGGGADGIARQQGTAQTGVKPETSPTARHGDFFAP
                     VAGGIGFKRRGLEGKTGGRRTV"
     gene            complement(9716..10027)
                     /locus_tag="WGQ71_001396"
     CDS             complement(9716..10027)
                     /locus_tag="WGQ71_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ0707395.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(10024..10392)
                     /locus_tag="WGQ71_001397"
     CDS             complement(10024..10392)
                     /locus_tag="WGQ71_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ0707396.1"
                     /translation="MRIFKNQWIVKFAKKHKISDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            complement(11206..11291)
                     /locus_tag="WGQ71_001398"
     tRNA            complement(11206..11291)
                     /locus_tag="WGQ71_001398"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11255..11257),aa:Leu,seq:gag)
     gene            complement(11305..11655)
                     /gene="secG"
                     /locus_tag="WGQ71_001399"
     CDS             complement(11305..11655)
                     /gene="secG"
                     /locus_tag="WGQ71_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ0707397.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            complement(11662..12435)
                     /locus_tag="WGQ71_001400"
     CDS             complement(11662..12435)
                     /locus_tag="WGQ71_001400"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ0707398.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            12619..13122
                     /locus_tag="WGQ71_001401"
     CDS             12619..13122
                     /locus_tag="WGQ71_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ0707399.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAA"
     gene            13224..13880
                     /locus_tag="WGQ71_001402"
     CDS             13224..13880
                     /locus_tag="WGQ71_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ0707400.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMAVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            13959..14282
                     /locus_tag="WGQ71_001403"
     CDS             13959..14282
                     /locus_tag="WGQ71_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ0707401.1"
                     /translation="MDIVQLGPAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWATEIDG
                     NMMRY"
BASE COUNT         3262 a         3572 c         4076 g         3597 t
ORIGIN      
        1 cgtctggaaa ttcccttaca atcacaccga tatttaaaga ctcattccca accttggcaa
       61 cggcacgata tagtggatta aatttagggg ctgtactaga ttagccctaa atcccacact
      121 aatcccgcag gattttaagc tgttgagacg gtgtgccgaa gttaaatcga aattcgcatt
      181 ctttcaagaa cagcgggaaa gatttacgat cgattccgtt gtattttcgc aagacgcgtt
      241 ttgcctgatt ccaaaagttc tcaatgccgt taatgtggtt ctgacggtct gcaaattcct
      301 tggaatggtt gatgcggtaa tgaatgaaat cgctcacgtc caacttgtcg tagctgctca
      361 gactatcggt ataaacaatg ctgtccggca tgattttctt cttgatgaca gggagtaacg
      421 tttcagactt ggcattatcc accacaacgg tatagacccg tccgttgcgt ttcagaatgc
      481 cgaagacaac cacttttcct gccgcaccgc gaccacgtct gcctttacgc cgtccgccga
      541 aatcgctttc gtccggctcg acagggccct caaaaacctc atcggcagcc aaggccaaat
      601 gatggttgat aaccgtgcgg attttacggt agaacagtgc tgccgaattg ggatggatac
      661 ccaaaatatc ggcggcagaa cgggcggtaa cttccagcac aaaaaaacgg agcagttctt
      721 tctgtacttt tttctttaat ttgcagtgcg ttatcttcat atttcgaggg taacatatct
      781 gctaatctag tacagcccct aaaatttaat ccactataat gccgtctgga atgcggcgga
      841 ttggggttgg gagcgtatag gggaagtgct tgtgcggaaa tggcgaaggt gtgtttttga
      901 ctccggtccg ccctgtttag attgctgtaa aaccatgccg tctgaaacgg aaaggtttca
      961 gacggcatgg tttttgattg gggcggtgcg ggtcagcctc cgagcagtgt ccacaaatcg
     1021 ttttgcaggt cggcttcgga ttcgccctcg gcaggtgcgg cgcggatata gccgccgtcg
     1081 ggctgcatca gccacgcgtg cgtattgtct gccaatgcca tttccaaacc ttcgcggatg
     1141 acgcgttttt tgagttcggg cgcggtaatc ggcgcggcgg tttcgatgcg gcggaagaag
     1201 ttgcgcccca tccaatccgc gctggagata aaggtatcgt ccgcgccgtt gttgtggaag
     1261 caatacacgc gcgcgtgttc gagctgcctg ccgatgatgg agcggacgcg gatgttttcg
     1321 gacaagcctt ttacacccgg gcgcaaggtg cacataccgc gcacaatcaa atcgatttgt
     1381 acgcctgccg cgcttgcccg atacagggct tcgatgacgg tcggttcgat gagcgaattc
     1441 atcttggcgg taatccgcgc cggtttgccg gcttttgcgt gttcggtttc gcgtgcgatg
     1501 cggtcgataa ccattttgtg cagggtaaac gggctttggt agagtttgtt cagccgcccg
     1561 ggtttgccca agcctgtgat ttccataaac aatgtgttca catcggcggt gatttgttcg
     1621 tcggcggtaa tgatgccgaa gtcggtgtag atgcgcgatg tgccttggtg gtagttaccc
     1681 gtgccgaggt gggcgtaacg cttgagtacg ccgtcttcgc ggcggatgac cagtgccatc
     1741 ttggcgtgga ctttgtagcc gaacacgccg tacacgacgt gcgcgcccgc ctcttcgagc
     1801 tgcttcgccc agttgacgtt gttggcttcg tcaaaacgcg ccatcagttc gacgacgacg
     1861 gttacttgtt tgccggcgag tgccgccttc atcagggcgc ggacgagttc ggaacgcgtg
     1921 ccggtgcggt aaatcgtcat tttgacggca agcacgtcgg ggtcggcggc agcttcgcgc
     1981 atcatttcga caacggggtc gaacgattgg taggggtggt gcagcaggat gggcgattgg
     2041 cgcaccaaat cgaatatgga cgcggttttg cccaaggctt tcagtctgcc cggcgtgtgt
     2101 gtgggaaatt tcaaatcggg gcggttgact aggtcgggga cggcgttgag gcgcacgagg
     2161 ttgaccgggc ctttgacctg atagagttcg gcggcggtca gtttgaattg cgcgagcaga
     2221 aagtcgcgga tgtaggcggg acaggtgtcg gcgacttcga gccgcacgcc gtcgccgtat
     2281 tcgcggtcgt gcagttcgtt ttggatggcg gcgcggaggt tttgcaggtc ttcttcgtca
     2341 acggtcaagt cgctgtcgcg cgtcaggcgg aactggtggc agcctttgac gttcatgccc
     2401 gggaagagtt tgccgacgtg ggcgtggagg atggacgaca ggaagacgaa gccgtgtccg
     2461 ccgccacaca gttcggacgg caggggaacg acgcgcggca ggatgcgcgg tgcctgcaca
     2521 atcgccatcc ccgaaggcct gccgaatgcg tccgtgccgt cgagttcgac ggcgaagttg
     2581 agcgatttgt tcagcggtcg cgggaagggg tgggacgggt cgagtccgat gggggtcaga
     2641 atcggcagca attcgcggtc gaaatagctt tcgatccact ttttctgcgc gcccgtccag
     2701 ttgcggcggc ggtaaaaatg gatgccttct tgtgccagct cgggctgaag gacgttgttg
     2761 aacaggtcgt actggtgccg tatcagggag cgcgccgctt cggtaacgtc ggcgatggtt
     2821 tcagacggca ttttgccgtt gtccagcctg cgccgggggt gcagcttgtt ttcacgcttg
     2881 agccacgcca tgcggacttc aaaaaactcg tcaagattgg acgacacgat gcacaggaag
     2941 cgcaggcgtt ccaaaagggg gacgtttttg tcttccgcct gtgccaacac gcggcggttg
     3001 aatgccagca ggcttagttc gcggcagagg atgcggtttt gttcgggcat aaggttctcc
     3061 caaagatggt tgttgttcgg tcggagcggg caggcaatgc cgtctgaagg cttggcggca
     3121 tctgcgccgg tttcagacgg catgacgtgg cggacagtct gtttcaggca tcggggcaat
     3181 gtgaaaacag ataccggcgc gtgcggtatc cgtctgtttc aaatcacttc caacacaaaa
     3241 taatgacgca gtttttcgta caccgcatcg ctgacgttga tgcagccgtt ggtcataatc
     3301 ctgtcggaca cggacgggga ggcgatgcgt tcgttccgcc tttccgacgg tatctgattc
     3361 caaacgcggt gcagggcgaa aagaaaatca ccctcctgct tgaaaccgat gacttcgccg
     3421 ccgtatccgg gcttgtcggt actgttcagc gtcagcccga acgtcccctt gggcgtggca
     3481 gtgccgatga ggacggggtg gcactgacgg tcgtcagcga aacagagttc cgctttggat
     3541 gtatcgacga tgactttttt cttttggata taggcattga ccgcatccgg ctgcccctgt
     3601 gcatatacgg gggcaaccat cgataggtcg cacaatatcc ctaaaagcag gcgcaacggc
     3661 atagcggctt attggcggat gcgtttgggt ttggcgggca cgatttcgcg gatgatgact
     3721 tgcggctcgg ctttgggtgc tggcggcgga caggcggcat ctttcgggaa gacggggttc
     3781 cagtagaagc tgcgggcaaa tttgtcttta tcgaaaatca ctttgtattg gcaggtagta
     3841 acatcttcca cgccggaagt gttttcaggg tcggtaccta cgcccggggt atggaagtgg
     3901 aacaggtaat cccattcgcg cacgccgtac ataccttcgt cgtaatgcgg gcggcccagg
     3961 attttgtaga tgtcgtcttt ggtcaggccg gggcgcatca agtccaattc gtcataggtc
     4021 gggaatgtgc cgcgcttgtt gtcgagcgtt acggaatagg gtttcgggaa aaccggattg
     4081 tcggtcgtgc cgtcggcttt gacgttgctt ttggttgcgc aggcagacag gatgcccgcc
     4141 gccaatactg ccaagcccgg tttgacgatt tgtttgattt tcatatacag aatcctcttt
     4201 tacccgatgc ccgtctgcct tttgttcgga agagccgctt caggatggtt ttaaatattt
     4261 gattttgatt tttaatggta gcataaaaca acgggcgtat cagcaggttc ggcgtatttg
     4321 tgcggcatac ggggcgaagg gtttgccgtg tcgcccgcct cctgccggca atctgccgat
     4381 tcgccgcttc cggcaacctg ttataattga tgccgacttt atttttccaa cgtttttaag
     4441 cgaaaacaga atgactatgc aagaacaata ccggcccgcc gccatcgagc ctgcggcgca
     4501 gaaaaaatgg gatgatgccc gtattttcaa cgtctccgaa gacgcttcca aacccaaata
     4561 ctactgcctc tctatgttcc cctatcccag cggcaagctg catatggggc atgtgcgcaa
     4621 ctacaccatt ggcgacgtat tgagccgctt caaacgctta aacggcttca acgtcatgca
     4681 gcctatgggt tgggacgcgt tcggcatgcc ggcggaaaac gcggcgatga aaaacaacgt
     4741 cgcccccgcc gcatggactt acgacaacat cgaatacatg aaaacccagc tcaaaagcct
     4801 gggttttgcg attgactggg cgcgcgaaac cgccacctgc aaacccgaat actaccgctg
     4861 ggaacaatgg ctgtttacca agctgtttga aaaaggcatc gtttaccgca aaaacggcac
     4921 ggtaaactgg gactctgtcg atcaaaccgt ccttgccaac gagcaagtta tcgacgggcg
     4981 cggctggcgt tcgggcgcgt tgattgaaaa acgcgaaatc ccgatgtatt acttcaaaat
     5041 caccgattac gccgaagagt tgctcaacga tttggacaag ctggaacact ggccggaaca
     5101 agtcaaaacc atgcagcgca actggatcgg caaatctcgc ggtatgaccg tgcgctttgc
     5161 cgtttcagac gacagcaaac aaggtttgga aggcgattac gcgagattcc tgcaagttta
     5221 taccacccgc cccgacacgc tgatgggcgc gacttatgtt gccgttgccg ccgagcatcc
     5281 gctggcaacc gcagccgccg ccgacaaacc cgaattgcag gcatttatcg ccgaatgcaa
     5341 agcgggctcc gttgccgaag ccgatatggc gacgatggag aaaaaaggcg tgccgaccgg
     5401 tcgctacgtc gtcaacccgc tcaacggcga taagctggaa gtgtggattg ccaactatgt
     5461 attgtggggc tacggcgacg gcgcggtgat ggcggttccg gcgcacgacg aacgcgattt
     5521 cgagtttgcc accaaataca gcctgccgaa aaaacaggtc attgccgtcg gcgacaacgc
     5581 atttgacgca aaccaatggc aagaatggta cggcgacaaa gaaaacggcg tattggtcaa
     5641 cagcggcgac ttggacggca tgaattttca gacggccttc gacgccatcg ccgccaagct
     5701 gcaaagccaa ggcgcgggcg aaccgaaaac ccaataccgc ctgcgcgact ggggcatttc
     5761 gcgccaacgc tactggggct gcccgattcc catcgtccat tgcgaacaat gcggcgacgt
     5821 acccgtccct gccgaccaac tgcccgtcgt cctgcctgaa aacgtcgtac ccgacggtat
     5881 gggttcgccg ctggcaaaaa tgcccgagtt ttacgaaact gcctgcccgt gctgcggcgg
     5941 cgcggcgaaa cgcgaaaccg acaccatgga caccttcatg gaatcaagct ggtatttctt
     6001 ccgctacatg tcgcccaagt tttcagacgg catggtatcg gcggaagccg caaaatactg
     6061 gggcgcggtc gaccaataca tcggcggtat cgaacacgcg attctgcacc tcttgtacgc
     6121 gcgtttcttc accaaactga tgcgcgacga aggtttggtc aatgttgacg aaccgtttga
     6181 acgcctgctg acgcaaggca tggtcgtttg cgaaacctac taccgtgaaa acgacaaagg
     6241 cggcaaagac tggatcaacc ctgccgatgt cgagctgact ttcgacgata aaggccgccc
     6301 tgtttccgcc gttctgaaag cagacggact gccagtcgtc atcagcggca cggaaaaaat
     6361 gtccaagtcc aaaaacaacg gcgtcgatcc gcaagagctg attaacgcct acggcgcgga
     6421 caccgcccgc ctgttcatga tgttcgccgc accgcccgaa cagtccctcg aatggagcga
     6481 cagcggcgtc gaaggcgcac accgcttcct gcgccgcctg tggcgtaccg tttacgaata
     6541 cctgaaacaa ggcggcgcgg tcaaagcgtt tgcaggcaac caagacggtt tgtctaaaga
     6601 actcaaagat ctgcgccaca aactgcattc caccatcgcc aaagtcagcg acgactacgg
     6661 ccgccgccag cagttcaaca ccgccatcgc cgccgtgatg gaattgctca accaatacga
     6721 caaaaccgac accggcagcg aacaaggccg cgccgtcgcc caagaagtat tggaaaccgc
     6781 cgtacgcctg ctgtggccca tcgtgccgca catctgcgaa accctgtgga gcgaattgaa
     6841 cggcgcgaaa ctgtgggaag caggctggcc ggcagtcgac gaagccgcct tggtcaaatc
     6901 cgaaatcgaa gtcatggttc aagtcaacgg caaactgcgc ggcaaaatca ccgtcgccgc
     6961 cgacgcatcc aaggccgacc tcgaagccgc cgcccttgcc accgaaggcg cggtgaaatt
     7021 catggaaggc aagcccgcga agaaaatcat cgtcgttccc ggacgcttgg taaatatcgt
     7081 cgtctaagcc gcttttaagg tttagccata cggataaagg ccgtctgaaa cttggaaaca
     7141 gggtttcaga cggccttttt aaggcagatt gggttgtccg catgacagac ctcaaatata
     7201 gtggattaac tttaaaccgg tacggcgttg ccccgccttg ccctgctgtc tgcggcttcg
     7261 tctctttgtc ctgattttgt taatccacta aaacttaatc tcaaaatcca aaacatgaat
     7321 ttatttttcg atactgaatt ggggaagcaa caaaataaag caacccacaa aatccgtgta
     7381 atgagcgagg cttggttgga aaaaaacggc tactgcccct gttgcggaag caagccgatg
     7441 aagagatttg ccaataacaa acctgttgca gacctctttt gcccaaattg ccacgagcaa
     7501 tatgaattaa agagtaaaaa tcaaaaaacc ataggcaaca tcgtgcctga cggtgcatat
     7561 cacaccatgt tggagcgcat ccggtcagat accaacccca actttttctt tcttgcatat
     7621 aaaaaagcgg attactccat acggcaattg gtgcttgtac ccaaacattt catcacgccg
     7681 gatatgatta ttcccagaaa taaaggcatt aaaaaccggc cgcaccacat tatgtgttcc
     7741 atcaatctcg cccctttgcc tgaaagcgga aaaatattct taatagacga ttcccgcatt
     7801 atcgaacccg aaaccgttct gaaaaaatgg caatctaatc tgtttttacg caaccaaaat
     7861 gcggagcgca aaggttggct tttggctgtt atgaaatgta tcgaccaact ccccgaagaa
     7921 ttcacattgt cgcaaatgta tgaatttgaa aacaaactat ccatccaatt tccccaaaac
     7981 aaccatatca aagacaaaat ccgccaacag ttgcaaattt tgcgcgacca aaatacgatc
     8041 gaattcattg gtcgcggact ttacaaaaaa atcgacaaat tgcgtccgac ttccaaggcg
     8101 gtttgatttc aaatcatgat actgaattca caagggatat ttattccttt gctattctga
     8161 aaacagttga agttcagcgc aatattgccg aaaaaggcag cagccgtaaa aaagtcgggg
     8221 aattattggc aatttatgcg taatttcgac aaattggctg aagaagctgc cgaacacgcc
     8281 gctgaaggcg cggtgaaatt catagaaggc aagcctgcga aaaaaatcat cgccgtaccg
     8341 ggcagactgg cgaacatcgt ggtgtaaatc tttttaaaaa cattaaacaa tgttaagatg
     8401 ccgtccgaaa tccgttttgg attcagacgg tatttttttg aaatttaatt ttttaaggag
     8461 taaacctaaa tatgaaattt ccttccttcc ttccttcctt cctttaaacg gatactctgc
     8521 ctgtcggcag taatctcggt attgggggct tgtacggtcg ttgcagatgt ttacggtcag
     8581 gattccgcca caatgaatgc tgcggctgcc gaagattata tgaaaacggt tgagttgaac
     8641 aagtctgccg gcaatgtcga tactacatcc aaaacagccc gtagggtgca ggcagtattt
     8701 cgacgtatgt tgccttatgc cgatgcggca aataataccg gccataagtt tgactggaaa
     8761 atgacggttt tcaaaaacga tgagctgaac gcgtgggcaa tgcccggtgg aaaaatggcg
     8821 ttttatacgg ggatagtcga caaactcaag ctgaccgatg acgaaattgc cgccattatg
     8881 gggcatgaaa tgacgcacgc cctgcatgaa cacggtaaaa ataaggtcgg gcaacaaatt
     8941 ttgaccaata cggcggcgca gataggcacg cagattatat tagacaaaaa accggacact
     9001 aatccggaat tggtcggatt gggtatggat attttgggaa cgtacggtat taccttgcct
     9061 tatagccgca gcttggaaga agaagccgat gaggggggaa tgatgttgat ggcgcaggca
     9121 ggctatcatc cggcggccgc tgtcagggtt tgggaaaaaa tgaatcagga aaacgaccaa
     9181 aacggcttta tttatgctat tacctctact catccgacaa acaatgcccg tatagaaaat
     9241 ctaaaacggt tgttgccgac cgttatgccg gtttatgagc aaagtgtcag aaataagggg
     9301 cgcgttaatc cactataaaa aacgtcggcg ttaagggaag gggatcgaga atttgagccg
     9361 ttgtttcaaa atgccgtctg aaatcgtttg agattcagac ggcatttaaa cagttctgcg
     9421 cccgcccgtt ttgccttcaa gcccgcgccg cttaaatcca atcccccctg caacgggcgc
     9481 aaaaaaatct ccgtgcctcg cggtcgggga tgtttcgggt ttcacgcccg tctgcgccgt
     9541 gccttgctgt cgggctatgc cgtctgcacc gccgccgttt cggcttggtt tgatgcggtc
     9601 gggcatttgt gcggctacgc cctgatattc ggttcggcgg tcttgcctgt ttcgtcaggc
     9661 atattcagca gcgcgtctat tgtgaacttg ctgattttgc cgtttgccaa gtcgtctacg
     9721 caatggcttc gatgcccttg tttttgacga tggtcagcag cttcaacgcc gcgccgctgg
     9781 gctttttaac gccccgctcc caagccgaaa cgtgattttt tcccacgttg agatagatgg
     9841 cgaaagcggc ttgcgatagt gcctccttct ccctgattgc cttgatgtct ccgccgctca
     9901 acggtttgat ttcggtcagg caggacttgt caaagccgcg catagtcttt ttgtcgattg
     9961 cgccgatgtt gtaagcccct tccatcattt catgaatggc ggctaatgcc tcgcttttgt
    10021 atttcatgat tctatctccg tgataatgcc gtcttctttc aaagccgcca gctctgcccg
    10081 cgtgtatttc agataatatg cggcggcttt tcggtaaacg tccagttctt tatccgaaat
    10141 gttttctctg tcgttcttgg caaaggcgta aacgaaaaat gccctatctg cctgtttgaa
    10201 caatatcagg ctgcgatagc cgccgcttct gccttgtcct tgccttgcta tgcgctgctt
    10261 aatcacaccg ccgcctaaat ctgcgtctat cagcccgtta tccgccctct ctaccgcttc
    10321 cagcagctcg gaatcactga ttttatgttt cttggcaaat ttcactatcc attggttttt
    10381 gaatatccgc attgtgtagc cgttgattag tgatacttgg aattatacca gagacagaca
    10441 acacggcaag ctgccttttt tatgccccac aatttctccg tttatgccgc ctgaaacact
    10501 ggcggctaac ctgattattt tttaatcaat cgcttttaat ggctcaaatt gcgtttttag
    10561 cgcgtttcgc tgtcttccta ttccccccgt atataaccaa gcggtttaac cgccattcct
    10621 gctcaaaata ggcgcggttc cgtgcgtttt ggcttttcat tccgctacgc tcccactgcc
    10681 aagacggtga aaaaccggaa aaccccaaaa gttgttaccg attcgttacc atttgttacc
    10741 gcatttgtta ccgcatttgt taccaatttt ttatcttttt cttattttat agtgaattaa
    10801 atttaaaccg gtacggcaag gcgaggcaac gccgtaccgg tttaaattta attcactata
    10861 gtataggttt tttccaaaaa cccaaaaaaa accgctctgc atcttggacg gtttccccct
    10921 cattccctac ggtgcgtcag tattccccgc aagctatttc ccgcttggca ttcctgattt
    10981 ggtggtcgtt cagctttatt tttacggtat tttctcgcgg tattttcgga tgctgtaaaa
    11041 aatcaggtta gttcaattag ggcggattgg acgggattgc actgtcagga agggggacgg
    11101 caggataaca atcagcctga aatccttgta ttgattggaa ttggttgaca gcgttggatg
    11161 ggattgaaca aaaaacgcct gaaattttca ggcgtttttg tgtgttggtg ccgacagcga
    11221 gatttgaact cgcacagcct acggccacta ccccctcaag atagcgtgtc taccaatttc
    11281 accatgtcgg catttgaaaa actgttattt ctgctgctga ggaacagggg cagaaggttc
    11341 ggtattgctt acgggtttgg gtgcttgctg agtttgttgt acgttgctga agtccaaacc
    11401 gtgttttgtc gtgtgggtgt gaatatacac catagccatg caggttgcaa agaaaaatgt
    11461 tgctgcaacg gcggtcgagc ggctgaggaa gttagcgttg ccggcagagc cgaatacgcc
    11521 ttgcgcgctg ccgcttcccg atccgaaagt cgcgccggca tccgcgcctt tgccgtgttg
    11581 gagcaatact aacacgatga cggccaaagc ggaaattata ttaacaatcc aaattagggt
    11641 tttgaaggct tccatatttt tctacgcatt ttgtgcggca ctgatgatgg cggtaaagga
    11701 gtcgtacgac aatgacgcgc cgccgacgag tgcgccgtcc acataaggta ctgcgaagat
    11761 gtcggccgcg ttgtccgctt tcacacttcc gccgtaaagg acacggattt taacatcgct
    11821 tccgcacaaa gacaagattt ctttgtagat gaatgcgtgc atatcggcaa tctgttcgac
    11881 ggtggcgact ttgccggtgc cgatcgccca gacgggttcg taggcgacgg cgatgttttt
    11941 ggtatccagc ccttgcagga tggaaagctg atgggcgatg acttcgtgtt ctttgccggc
    12001 ttcgcgctct tcgaggcttt cgccgacgca caataacggg atgagtccga cgttgaggac
    12061 gttttccatt ttgcggcgtt ggatttcgtt tttttcgccg aaataaaggc tgcgttcgga
    12121 gtgtccgatg aggacgatgt ctgtgccggt gtcggcgagc atttcggcgg acacttcgcc
    12181 ggtgtacgcg ccgttgtcgg ggaagcggct cacgtcttgg gcgcaggtga ggatgcggtt
    12241 gtttaagacg atttgcatgg cgttgtgcag ttgcagcagg taaacggtcg gggcggcgag
    12301 tccgatgagg acgcgttcgg cggtggggag gatgcggaag cggtgcatga gcgaattgtt
    12361 gttttggagc cggccgttca ttttccagtt gccgatgacc catttttgat cccacattcc
    12421 gatttggtga tacatctttt ttgctccgtg tcgtgttttt tctgtctgcc gcgtgtggcg
    12481 cgttgcaatg tgaagtttag tggatatgcg gcgggttcgc aacttggggc gggcggctgc
    12541 gggggcggtt tggaatgttg tttcgggcag gttgttttat aatggccgcc tgatatgtat
    12601 gcaactatag gagatgtgat gcacgcgctt catttttcgg cttcggacaa ggccgcgctt
    12661 tatcgggagg tgttgccgca gattgagtct gtggtggcgg acgagacgga ttgggtggcg
    12721 aatttggcga acacggcggc ggttttgaag gaggcgttcg gttggttttg ggtgggtttt
    12781 tatttggtcg atacgcgttc ggacgaattg gttttggcac cgtttcaggg gcctttggcg
    12841 tgtacgcgga ttccgttcgg tcgcggggtg tgcggtcagg cttgggcgaa gggcgggacg
    12901 gtggtcgtcg gggatgtgga cgcgcatccc gaccatattg cctgttcgtc tttgtcgcgt
    12961 tcggagattg tcgtgccgct gttttcagac ggccgctgta tcggcgtgtt ggacgcggac
    13021 agcgaacatt tggcgcagtt tgatgagaca gatgctttgt atttgggcga actggcgaag
    13081 attttggaga agcggtttga ggcttcgcgc caggcggctt gagtgaggga aaggggcggg
    13141 cggcgcgtgc cgaagttggc gcggcgggag tgtggtttta taatgcctgc cattgttgaa
    13201 acaattattt gacggagcac taaatggatt ttgaaaaagc gcggttcaat atggtcgaac
    13261 agcaaatccg tccttgggat gtattggatt ttgacgtttt ggacgctttg gcggagattc
    13321 cgcgcgagct ttttgtcgat gaggacttgc agggtttggc gtatgcggat atggcgctgc
    13381 cgcttgccaa cggtcataag atgctcgagc cgaaagtcgt ggcgcggctg gcgcagggct
    13441 tgaagctgac gaaaaacgat acggttttgg aaatcggcac gggttcgggc tatgcgaccg
    13501 cgctgttggc aaaattggct ggtcgtgtgg tgtcggacga catcgatgcc gaacagcaaa
    13561 accgcgccaa agcggtgttg gacggcttgg gtttggacaa tatcgattat gtgcaaaaca
    13621 acgggttgac cgaactttcc gcaggtgcgc cgtttgatgc ggtttatgtc ggcggtgcgg
    13681 taaaccttgt gcctgaagtg ttgaaagaac agttgaaaga tggcggacgc atggcggtta
    13741 tcgtaggccg caagcctgtt cagcgcgcgc ttttgattac gcgcaagggc gatgtgtttg
    13801 aagagaaggt gctgttcgat accttggtgg cgcatttgga tgataaggat gccgatccgt
    13861 ttggcggttt tcagttttga tgttcggatt gcgatgccgt ctgaaagcgg gtttggggct
    13921 tcagacggca ttttgcttgg ttttttggga tttttgcgat ggatattgtg caacttgggc
    13981 cggcggaatt gaaggcgtgg atggacgaag ggcggatgtt ttgtttgttg gacgtgcgta
    14041 cggatgaaga aacggcggtt tgttcgctgc caaatgcgct gcatatcccg atgaatctga
    14101 ttccgctgcg gcagaacgag ctgccggacg atgtgccgct tgtggtgtat tgccatcacg
    14161 gtatccgcag cctgcatacg gcgatgtatc tggcggacgc gggttttgaa aacctgtaca
    14221 accttcaggg cggcatcgat gcatgggcga ccgagattga cggcaacatg atgcgttatt
    14281 gaggcaggca tttcagacgg cattccttaa atgcgtgtac ctgtggtgtt ccaaaaggtc
    14341 gtctgaaagt gcagcttctg cgaagctaaa gcgcagtatc aacgaagtta aaatttgcct
    14401 gaaccttaaa ggcagcctgc accccaatcc cctctccccg tgggagaggg ctaggacgag
    14461 gctggcaaac tgaaagtttg cttgggcggc attttcaatg tgcaggc
//
LOCUS       DAWFIE010000052        14231 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWFIE010000052 DAWFIE010000000
VERSION     DAWFIE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14231
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.052"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..164)
                     /locus_tag="WGQ71_001404"
     CDS             complement(<1..164)
                     /locus_tag="WGQ71_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0707402.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPA"
     gene            351..533
                     /locus_tag="WGQ71_001405"
     CDS             351..533
                     /locus_tag="WGQ71_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707403.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNAGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(595..1596)
                     /locus_tag="WGQ71_001406"
     CDS             complement(595..1596)
                     /locus_tag="WGQ71_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ0707404.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1673..5506)
                     /locus_tag="WGQ71_001407"
     CDS             complement(1673..5506)
                     /locus_tag="WGQ71_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ0707405.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRTALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAADKAVGRFDGSFDETRQKRQQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVCALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHNGVEFVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWEWLLANLDAPLADYKARYGAA
                     VHAAPEAKDNESCFIAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGTA
                     SIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5767..5863
                     /gene="ffs"
                     /locus_tag="WGQ71_001408"
     ncRNA           5767..5863
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WGQ71_001408"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            6031..7722
                     /gene="dld"
                     /locus_tag="WGQ71_001409"
     CDS             6031..7722
                     /gene="dld"
                     /locus_tag="WGQ71_001409"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ0707406.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGRALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNQQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHGGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            7854..7961
                     /locus_tag="WGQ71_001410"
                     /pseudo
     CDS             7854..7961
                     /locus_tag="WGQ71_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            8017..8388
                     /locus_tag="WGQ71_001411"
     CDS             8017..8388
                     /locus_tag="WGQ71_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ0707407.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8543..9091
                     /locus_tag="WGQ71_001412"
     CDS             8543..9091
                     /locus_tag="WGQ71_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0707408.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDASYNQQQAVEVVMGVALA
                     TLCNYVNNLAQTEINPELQAYA"
     gene            9221..10312
                     /locus_tag="WGQ71_001413"
     CDS             9221..10312
                     /locus_tag="WGQ71_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0707409.1"
                     /translation="MNAQTLIANVAELVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDK
                     YLADILQGKVLAGTGMSNTVKHLAGIEKHNLQAERVEGGYTVNGALPWVSNIGEEHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNSYLDDGYDSLKARLD
                     GAWAETERLAGLAWSGTPDNLATPKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10783..10953
                     /locus_tag="WGQ71_001414"
     CDS             10783..10953
                     /locus_tag="WGQ71_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ0707410.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
     gene            12234..13976
                     /locus_tag="WGQ71_001415"
     CDS             12234..13976
                     /locus_tag="WGQ71_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ0707411.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVETAVLATLLF
                     ATVQANATDTDEDDELEPVVRSALVLQFMIDKEGNGEIESTGDIGWSIYYDDHNTLHG
                     ATVTLKAGDNLKIKQSGKDFTYSLKKELKDLTSVETEKLSFGANGNKVNITSDTKGLN
                     FAKETAGTNGDPTVHLNGIGSTLTDTLAGSSASHVDAGNQSTHYTRAASIKDVLNAGW
                     NIKGVKTGSTTGQSENVDFVRTYDTVEFLSADTKTTTVNVESKDNGKRTEVKIGAKTS
                     VIAEENGKLVTGKGDEGKGLVTAKTVIDAVNKSGWRVKTTDNNQTGQADKFETVTSGT
                     NVTFASGKGTTATVSKDDQGNITVKYDVNVGDALNVNQLQNSGWNLDSKAVAGSSGKV
                     ISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSSVSLGAGADAPTLS
                     VDDEGALNVGSKDTNKPVRITNVAPGVKEGDVTNVAQLKGVAQNLNNRIDNVDGNARA
                     GIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGGNWIIKGTASGNS
                     RGHFGASASVGYQW"
     gene            14109..>14231
                     /locus_tag="WGQ71_001416"
     CDS             14109..>14231
                     /locus_tag="WGQ71_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0707412.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQ"
BASE COUNT         3550 a         3836 c         3730 g         3115 t
ORIGIN      
        1 taggcgggac ggccgcggtg gtctcggagg taacgggttc tttgacgatt caggtactgt
       61 tcgatcggct gccaatcaat cacctggtcc aacttcaata gtgggaaccg gtcgatgtgt
      121 ttagcaatca tggcttgggc ggtttgctgg aagaaggtgc tcatgagaaa tcccctaaat
      181 gtcttggtgg gaatttaggg gattttgggg aattttgcaa aggtctctat ttgtactgtc
      241 ttcggctcgc cgccttgtcc ttatttaaat ttaattcact atatttgccc gtgcgtttgt
      301 gataaattcg gcactgtatt tgatatgatt ttaacaaatc gggaggggca atgttttggt
      361 atgtaatcgg cttttgctcc tttgtcgtcg cgctgctgtc gctgtgggtc aatgccggcg
      421 cattcggtat gcaggaagac gataccccgc aatcggatta tgaaaaacgt ttgggcttgg
      481 gtgcgaagct gaaaaacaaa aatacgccgg atacggaaga aaagcagcaa taaagatatg
      541 aaatgccgtc tgaaagattt tcagacggca tttgctttga agcatgggag acagtcaaac
      601 atttatctca tgctcaaatg cgttcaggat tcggcggact tcatccgcat ccttaaccgt
      661 tcggacgcgg tcaaacagcg tttgcgccgg gtcgaacgtt tttttcatca tgcccaacca
      721 ttgcttaaga cgggcgatcg ggtatttatt gtttgcctct tttgtcaggc acagctcgaa
      781 aaactgccgt atccatttgg aaacttcggc aaaatccgtg tctttgaccg gcccgccgtt
      841 ctcgtattgc ttgatttgcc gcgccaaatc ggggcggatg accgcgccgc gaccgagcat
      901 cacgctgttg cagccgctga ttgttttgat gccgatatag tcttgcaggc tgaaaacgtc
      961 gccgttggcg gtaacgggaa tattgacgct gtctcggatt ttccttatcc attcccaatg
     1021 cgccggcggt tcgtaaccct cggctttggt acgcgcgtgt acggtcagtc cgcatgcgcc
     1081 cccttcggca atcgcacagg cgcattccaa agcccggctt ttgtcttcgt aaccgagccg
     1141 catttttgcg gtgagcggaa tatgtgcggg caaacgtccg cgcagcgttt tgacgatgtg
     1201 gaatatcagt tccggctctt ttaaaaggat tgcgccgcct ttgtgtttgt tgacggtggg
     1261 cgcggggcag ccgaagttca aatcgatttt gttcgcaccg aagcggacgg cttccagcgc
     1321 attcgccgcc atattgtccg catcgctgcc caaaagttgg acggtgcagg gcgtgccgga
     1381 aaacgttttg tttccgttgg cgatttcggg gacatatttt aaccatatgg atcgggaatg
     1441 cacggtatgg gtaatgcgta caaattcgct gacgcattcg tcgtagccgc caatacgcgt
     1501 cagcaggtcg cgcatcacgt catcgaccag accctgcata ggggcaagga tgattcgggt
     1561 tttttgtttg ggttcggttg tttgtccgtc aatcatgatt tgggtaggta ttgggtttaa
     1621 acaatgccgt ctgaaggctt tcggcgttca gacgacattt ccgtgtccgt tgttacagca
     1681 atactttttc cacgccgccg ttgttggctt ttttcacaaa ctcgtctagc cagttttcgc
     1741 cgagaatgtg tttcgccatt tcgatgacga tgtagtcggc aggcatattg ttgtcgtcgg
     1801 cgtagcggct caaaccttgc aggcaggcgg ggcaggaagt cagcattttg acgggttcgc
     1861 cctgcggcag ctctttgagg tttttctcga tttcctcttg tttgcggaac ttgacctgag
     1921 tggcgatgtc tggccgtttg acggcaaaca taccggattc gccgcagcag cggtcgctta
     1981 aaacgacttt ctgtcccatc aggctgctgg ccatttgggt ggcgttcatg gttttaatcg
     2041 gggtgtggca ggggtcgtgg tagaggtatt gctgaccttt cacgccgtcg agtttcacgc
     2101 ctttttcgag cagatattcg tggatgtcga tgatgcggca gccggggaag atttcttcaa
     2161 agcggtattt ttcgagctgg tcgtaacaag tgccgcaact gacaacgacg gttttgatgt
     2221 cgaggtagtt gagggtgttc gccatacggt ggaaagccac gcggttgttg gtgctcattt
     2281 cttcggcttt agccttgttg ccgcccgcgt cttgcggata gccgcaacac atatagccgg
     2341 gcggcaggac ggtttgtacg ccgacgtgcc agagcatggc ttgaacggca agtccgattt
     2401 ggctgaacag acgctcagag ccgcaacccg ggaagtagaa cacggcttcg gcatcttcgg
     2461 gcgcggcggg gttgcggatg atggggatgc ttttgccgtc ttcgatgccc aataaggagc
     2521 gcggtgtttt ggcgggtacg cttttcggca aaggacggtt gatgaaatgg ataacctgtt
     2581 ctttgattga ggcagtaccg acggttgcct tgggttcggc tttttgcttt ttcgtgccga
     2641 ttggaagaag tttgccgatt ttgtaggcaa agttctgcgc ggggaagccg gtctgtatca
     2701 tcgcggcgcg aagggctttg atggttttcg ggccggtggc gttcaaaaac gccatgccca
     2761 tcgatgcggc gggtgcaaat cgtttgtggc cggaatcggc aaggtagttg cggacggcta
     2821 cggttacgtc gccgaagtcg atgttgacgg ggcagggttt gacgcagcgg tggcacacgg
     2881 tgcagtggtc gccgatgtcc atgagttctt caaagtgttt gacggaaacg ccgcggcggg
     2941 tttgttcttc gtataagaag gcttcggtta acagccctac gccgaggatt ttgttgcgcg
     3001 gactgtacag caggttggcg cgcggaacgt gggtggagca gacgggtttg catttgccgc
     3061 agcgcaggca gtctttgacg gaatcggcaa tcgtgccgag gtctgatttt tccataatca
     3121 gcgattccgc gcccaaaagc tcgaaagacg gcgtgtaggc gttgcgtaag tccgagcctt
     3181 tcatcagttt gtgacggttg aaggtgtgtt tgggatcgac ttggtttttg tagtcccaaa
     3241 acggctgcat ttcttcgtcg cttaaaaatt cgagcttggt aatgccgatg ccgtgttcgc
     3301 cggaaatcac gccgttaagc gaacgggcga ttttcataat gcgttcgact gagcggtatg
     3361 ccgtctgaag catttcggca tcgtctgagt taaccggaat attggtgtga acgttaccgt
     3421 cgccggcgtg catatgcagg gcgacaaaga cgcggctgcg tacggttttg gcgtggattt
     3481 ttcccaagcc ttggataatt ttggtgtcgg ttttgccgct gaagatttca gaaagcggct
     3541 tcattacgtc cgctttgaca gacacgcgca ggcggaaatc gcggaaggca ataaagcaac
     3601 tttcattgtc tttggcttcg ggcgcggcgt ggacggctgc gccatagcgt gctttatagt
     3661 cggcaagcgg cgcgtccaag ttggcaagca gccattccca acgcgctttg acggcggaaa
     3721 cgtgggcaag ggcgtgtttg ccgcgttcgc ccaacagttc ggcggtcggc aggtctgttc
     3781 ccattttgtc gatggggagt ttgcccgaaa gatattgctc caaggcggca cagagtttga
     3841 gtttgttttg gatggaaagc tcgatgttga tgcgttcgat gccgtctgaa tactcgccga
     3901 gcctttcgag tgggatgacc acgtcttcgt taattttaaa ggcgttggtg tgcttggcga
     3961 tggcggcggt gcggctgcgg tcgagccaaa aggttttgcg cgcttcggga gacacggcga
     4021 taaagccttc gccatcgcgg gcgcgggcaa gttcgcaaat gtgttcggcg gctgcctcta
     4081 cggcggcttc gtcgtcggaa accacgtccg ccagcaagac cattttcggc cgtcccttgc
     4141 ccgccgcttt ggttgcgtag ccgacggcgc ggacgtaacg ccagtccaaa tgttccaaac
     4201 ccgccagccg cacgctgtcg tgggcaagca ggaaatcgcg gatttcgacg atagaaggcg
     4261 tagcggtggc gaccgtaccg aaaaactcca tacacacggt gcgcgtgtat ttcggcattt
     4321 tatgcaacac gaaggcaacg ctggtgatga tgccgtctgt accttctttt tgcacgccgg
     4381 gcaggccgct caagaatttg tcggtaacgt ctttgcccaa accgactttg cggaatttgt
     4441 gaccggggat ttccaaacgt tcggttttaa cgatattgag gccgtctgaa tccagcgtgt
     4501 gaacgtcgaa cacggcggtt tcttcgtcgt ggattttgcc gaaattatgg cgcacgcgct
     4561 cgatgcgcag ccattcgccc tgagggttga ccatctgcca gtaggcgaga ttgtccaacg
     4621 ccgtccccca cagtacggct tttttgccgc ccgcgttcat cgccacattg ccgcccacgc
     4681 aggacgcatc ggcggaagtc ggatcgacgg cgaataccaa acccgcctga tgcgcggttt
     4741 cttccacccg ccgcgtaacc acgcccgcgc cgcaccggat aatcggatgt ttgccgtcca
     4801 agcccgccag ttcgacaaat tccacgccat tatgtttatc cagcttttcc gtattgatga
     4861 ccgcgctcat cgcgtccaaa ggaatcgcgc cgccggtata acccgtgccg ccgccgcgcg
     4921 gaataatgac caaatccagc tcgattaagg cgcacaccaa aggcgcgatt tccgcctccg
     4981 tgtcgggatt gacgaccaca aacggatact ccacgcgcca gtcggtcgca tcggtaacgt
     5041 gcgttacccg cgccagcccg tcgaacataa tattgtgcgg cttggtgatt ttgctcaaac
     5101 gctccaaaat ctgctgccgc ttttggcgcg tttcgtcaaa gctgccgtca aaacgcccga
     5161 ccgctttgtc cgccgccgca accaagacat ccacttgccg attgtcgtcg cggcgtttgc
     5221 ggatttcgtt caaacggtgg cgcatttccc gcaccagcgc ggtgcggcgt ttcgggtgct
     5281 ccaacaagtc atcgaccaga tatggattgc gcacaactac ccaaatatcg cccaacactt
     5341 caaacagcat ccgcgccgaa cgtccggttt tacgctgtcc gcgcaagtct tgcagaatct
     5401 gccacgcctc gtcgcccaat aatcggatga cgatttcgcg gtcggtgtag gaagtatagt
     5461 tgtaggggat ttcccgaata cgctgagggg cggtagtcgt ggtcatggtg tgtccgtgtt
     5521 tggcagtttt tatttgaatg ccgtctgaaa agtcggtttc aggcgagttt gaaaaggcga
     5581 taatgtagtt caatttttga tacttttcaa tgccgaaaca ggaaataccc ttatagaaac
     5641 aaaataacgc tttgagaaat tgttgacaat attatcaaat ataaatttta ggtggtgttt
     5701 ccggcgtatg accgtatcaa aacggttgag tttttcagaa aacccgttat aatgccgttt
     5761 ctctgaggcg ggtctccccg catggcaaat cggaacaccg ggtcaggggc ggaagccagc
     5821 agcccactcc gatgcgccag tgccgggggt ttggcccgcc gccctatttg aaacgccgga
     5881 gcttcgatgt tccggcgttc ccgtatcgca ccgatgccgt ctgaaagccg ttcagacggc
     5941 attttcttta ctttgccata acgtataact acaatgggaa aacaatttca atgtgcatat
     6001 gcacctgccg acaattttaa ggaaactgcc atgagcgcat cacaattact gtcaagactg
     6061 acccaaaccg taggcgaaaa atacatcatt accgaccccg cgaaaaccga acaataccgc
     6121 caaggctacc gcttcggcga aggcagggcg ttggcggtgg tgcgccccgg aagcattctg
     6181 gaaatgtgga aaattctgca ggcgtgcgtc gaagcagacg tgattgtgat tacgcaggcg
     6241 gcgaataccg gactgaccgg cggttcgacc cccgacggca acgattacga ccgcgacatc
     6301 gtgattgtga acaccatgcg gatgaacatc atccaaacca tcaacaacaa ccaacaagtc
     6361 gtctgcctgc ccggctcgac cctgaaccag cttgaattgc tgctgaaacc tttggggcgc
     6421 gaaccgcatt cggtcatcgg ctcatcctgt atcggcgcgt ccgtcttggg cggcgtgtgc
     6481 aacaactccg gcggcgcgtt ggtacagcgc ggcccggcct acaccgaaat ggcgttgttc
     6541 gcccaaatca acgaagaagg caggttggaa ctggtcaacc acttgggcat agacttgggc
     6601 gatacgccgg aagaaatcct gaccaacctg caaggtcatc attatcaaaa caaagacatc
     6661 aaacaagacg cgggcaaagg acacgaccac gcctattgcg aacacgtccg ccaagtggac
     6721 gaaccgaccg ccgcgcgttt caacgccgac cccgcgcgcc attacgaagc ctcgggctgc
     6781 gcgggcaaac tgatggtgtt cgccgtccgt ttggacacct tcccgcaaga aaaacaaacc
     6841 gccgtgttct acatcggcac caacgacatc aacgagctga ccgacatccg ccgcgccgcc
     6901 ttgggcgaat ttgaaagcct gcccgtttcc ggcgaataca tccaccgcca cgctttcgat
     6961 attgccgacg tgtacggcaa agacacgttt tacgtcatca aaaaattcgg tacgcaccaa
     7021 ctgccgaaat tatttgactt gaaggcgcgc gtggacaggt tcggcaaaaa agtcagcttc
     7081 ctgcccaaac atttttccga caaggcaatg cagttcgtca gcaaattcct gcccgaccac
     7141 ctgcccaaat ccatgcgcga ttaccgcgac aaatacgaac accacctgat tctgaaaatg
     7201 ggcggaaaag gcgtggatga agcgcgcgcg ttcttaaaag aatattttgc acaccacggc
     7261 ggcgcgtttt ttgaatgcaa cgccgaagaa acccaagccg cgatgctgca ccgttttgcc
     7321 gtcgcttccg ccgccatccg ctaccgcgcc gtgcatgatg atgaagtgga agatttggtc
     7381 gcgctcgaca tcgccctgcg ccgcgacgac cgcgattggt ttgaaaaact gccgccggaa
     7441 attgacaata aaatcatcca taaattgtat tacgggcatt ttatgtgcca cgttttccat
     7501 caggattaca tcatcaaaaa aggcaacgac tgcatggcgt tggaacacga aatgctgcat
     7561 ctcttagacc aacgcggcgc gcaatatccc gccgaacaca acgtcggtca tttgtatgaa
     7621 gccaagcccg cgctcaaaca gttttaccgc aaactcgacc cgaccaacag cttcaacccg
     7681 ggtatcggca aaaccagcaa aaagaaaaac tgggcggaat aagcgcgtcc gctttgaagg
     7741 caggcaatgc cgtctgaagg caaaacgccg ttcagacggc attgtcgggt ttgaagttcc
     7801 ccgtcgggta atcgctttca aaccgtatgc cgacacacgg gcgcgcccct cccaaatata
     7861 gtggattaaa tttaaatcag gacaaggcaa cgaagccgca gacagtacag atagtacggc
     7921 aaggcgaggt aacgccgtac tggtttaaat ttaatccact ataaagtctg atcctacctt
     7981 cccaaagggc agggtttcaa ccgaaaagga aacacgatga aaccatacaa aatctacacc
     8041 catcccgccc tgccgcccca agccgtcaaa caaggctggt catggccggg gctcttgttc
     8101 ggcacgctgt gggcgtgttt caaaagaatg tggggtttgg ggctggggct gacgggcgcg
     8161 atattcgtgc tggctgtgtt tgcccaactg gtttacggcg acacgcccgc cacagactcg
     8221 gcgtttaacg tattgggctt ggcggtttcc gtctggttcg gcgcaaaggg caacagcctt
     8281 tatgcacgcc accttttatc gcgcggctat accgaattgc ccgaaacggt tgaggcagcc
     8341 aatccccaag ccgcattggc gcaatatttc gggcgcgggg gccgataagc gcgttttctt
     8401 ttccatcgtg ccgtctgaaa aatgttcaga cggcatttct tataaccttt cgtttgcagc
     8461 acataaccaa tcagtctttc ctattccgtt ccgaaatgca taccatgcgc ccgtacccaa
     8521 tatttgcgaa caaggaaaga aaatggcacg tttaaccgta cacaccctcg aaaccgcccc
     8581 cgaagccgcc aaagcgcgcg tcgaggcggt acttcaaaac aacggcttta tccccaacct
     8641 tatcggcgta ttatcaaacg cccccgaagc cttggcgttt taccaagaag tcggcaagct
     8701 caacgccgcc aacagcctga ccgccggcga agtcgaagtc atccagatta ttgccgcccg
     8761 caccaaccaa tgcggcttct gcgtggcagg gcacaccaaa ctcgcaaccc tgaaaaaact
     8821 cctttccgaa caatccgtca aagccgcgcg cgctttggcg gcaggcgaat ttgacgatgc
     8881 taaactcggc gcgctcgccg cctttaccca agccgtaatg gcaaaaaaag gcgcggtatc
     8941 cgacgaggaa ctcaaagcat tttttgatgc gagctacaac cagcagcagg cagtcgaagt
     9001 cgtgatgggc gtagccttgg caactttgtg caactacgtc aacaacctcg cccaaaccga
     9061 aatcaacccc gaattgcagg cttacgcctg atacggcaaa ccgcccgaat atcgggcggt
     9121 ttttcaaccc ccctcaaaca aaaacaagcc gccaaacggc agggaagcag ccgcgcatca
     9181 ggcgtgcgcc cgatttcccc gcaaacctta aggaacaaag atgaacgccc aaaccctgat
     9241 tgccaacgtt gccgaactcg tcaaaaccaa gctcaaaccc atagtggacg acatcgaccg
     9301 caaaggatac taccccgaag catttatgcg cgaactcggc gcaatcggcg gattcggcgc
     9361 agtcggcaca gaagccgaag gcggcaacgg cttgggtttg gcaacgcaaa tcgccgtatt
     9421 gcgcgaaatc ggcaaagaat gcggcgcaac ctccttcagc gcgtggtgtc aggcggcttg
     9481 cgcgtggtat ctgcatcaaa cgcccaacca agccgtcaaa gacaaatacc tcgccgacat
     9541 cctgcaaggc aaagtattgg caggcaccgg aatgtccaat accgtcaaac accttgccgg
     9601 cattgaaaaa cacaaccttc aagccgagcg cgtggagggc ggttatacag tcaacggcgc
     9661 gctgccgtgg gtatccaaca tcggcgaaga gcatatttgg gcaaataccg cccaaatcgg
     9721 cggcggctac gtcatgttta tcaccggcgg acaatgggaa ggcgtaagcc tgcaaaactg
     9781 ccccgaattt tgcgccctcg aaggcacgcg caccttcagc ctgaacttca aagacgtatt
     9841 tatccccgac gaagacatca tcgccgcgcc cgaacagttt gccgactaca tccaaagcat
     9901 caaagcaggc tttatcctcc tgcaaatcgg catcggcgcg ggcgtgatag acggcagcct
     9961 cggcatcatc cgcctcgcca acatcgtcaa cgccgaagtc aacagctatc tcgacgacgg
    10021 ctacgacagc ctcaaagcaa ggctggacgg cgcgtgggca gaaaccgaac ggctcgccgg
    10081 attggcttgg agcggcacgc ccgacaacct tgctaccccc aagctgcgcg aagccgccgc
    10141 cgtactggca cttgccgccg cccaatccgc cgccctgcat tccggcgcaa aaggctacct
    10201 gatgcgcagt cccgcccaaa ggcgcgtccg cgaagcaatg tttgtcgcca tcgtaacccc
    10261 cgcgatcaaa cacctccgaa aagaaatagc ggcaatcgaa gccgcaaaat aaacaggcgg
    10321 aaaaaaccga aacccccacc gcgtcattcc cgcgaaagcg ggaatccagc ccccaacgca
    10381 acaggaatcc atcggaaaaa acagaaaccc ccaaaccgtc attcccgcaa aagcgggaat
    10441 ccagccccct aacgcaacag gaatccatcg aaaaaaccga aacctccaaa ccgtcattcc
    10501 cacggaagtg ggaatccagt aaccgaaaaa ccacaggaat ctatcgaaaa aaaccccacc
    10561 ccccaaaaaa accgggcgga tacctcacca tcctcccaaa ccctgattta tagcggatga
    10621 acaaaaatca gggcaaggcg gcgaaaccgc agacagtaca aatagtacga aaccgattca
    10681 cttggtgctt gagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgccgta
    10741 ccggtttttg ttcatccgct atacaaaaac aaggaaacac aaatggcgca atatatgtgc
    10801 ggcccctgcg gctggattta cgatgaagaa cacggcgacc ctgaacacgg catccccccc
    10861 gggacaaagt ttgaagacat tcccgacgac tggaaatgcc ccgaatgcgg cgtgggcaaa
    10921 gaagatttct acctgttgga tttcgtgata tagcggcagc cgaacccctt cggaaaattc
    10981 ctcggcgttc cgcctgcggt agcagggaac accgttttat agtggattaa caaaaatcag
    11041 gacaaggcga cgaagccgca gacagtacaa atagtacgga accgattcac ttgttaaaga
    11101 atcgttctct ttgagctaag gcgaggcaac gccgtactgg tttttgttaa tccactatat
    11161 ccgtttgcgc ccgccaagcc gtgttgcaca gatgcgacac ggcttttttc tttctgttgt
    11221 cagaatgtaa attttcgtca aaaaaacatc aaaaatcatc cccccccccg aatttgcgta
    11281 aaataaagtg taatcccttt gatcataagg gaatgttgac aattagatcg attctcctag
    11341 gctgactttc gcaataaatg gggttgtcaa taattgtgta tggtcgtttg ccgtccggcg
    11401 gccgtcattc ccgcgcaggc gggaatccag aaacgcaacg cagcaggact ttatcggaaa
    11461 aaaccgaaat cccaccgacc gtcattcccg caaaagcggg aatccagcaa cacaacgcag
    11521 caggaattta tcggaaaaaa cagaaacccc cacctaccgt cattcccgcg aaagcgggaa
    11581 tccagcaaca caacgcagca ggaatttatc ggaaaaaaca gaaaccccca cctaccgtca
    11641 ttcccgcgaa agcgggaatc cagacccgtc ggcacggaaa cttaccggat aaaacagttt
    11701 ccttagattc cacgtcctag attcccgctt tcgcgggaat gacgagattt tagattatgg
    11761 gaatttatca ggaatgattg aatccataga aaaatcacag gaatctatca gaaaaaacag
    11821 aaacccccac cgcgtcattc cccaccgacc gtcattcccg caaaagcggg aatccagcaa
    11881 ccgaaaaacc acaggaatct atcagcaaaa acagaaaccc ccaccgaccg tcattcccgc
    11941 aaaagcggga atccaaaaac acaacgcaac aggaatttat cggaaaaaac agaaacccca
    12001 ccgccgtcat tcccgcaaaa gcgggaatcc agacccgtcg gcacggaaac ttaccgaata
    12061 aaacagtttc cttagattcc acgtcccaga ttcccgcctt cgcgggaatg acgagatttt
    12121 aagttggggg aatttatcag aaaaccccca acctccaaaa accgggcgga tgccacacca
    12181 tccgccccca aaccccgatt taaccattca aacaaaccaa aagaaaaaac aaaatgaaca
    12241 aaatataccg catcatttgg aatagtgccc tcaatgcctg ggtcgtcgta tccgagctca
    12301 cacgcaacca caccaaacgc gcctccgcaa ccgtggagac cgccgtattg gcgacgctgt
    12361 tgtttgcaac ggttcaggcg aatgctaccg ataccgatga agatgacgag ttagaacccg
    12421 tagtacgctc tgctctggtg ttgcaattca tgatcgataa agaaggcaat ggagaaatcg
    12481 aatctacagg agatataggt tggagtatat attacgacga tcacaacact ctacacggcg
    12541 caaccgttac cctcaaagcc ggcgacaacc tgaaaatcaa acaaagcggc aaagacttca
    12601 cctactcgct gaaaaaagag ctgaaagacc tgaccagtgt tgaaactgaa aaattatcgt
    12661 ttggcgcaaa cggtaataaa gtcaacatca caagcgacac caaaggcttg aattttgcga
    12721 aagaaacggc tgggacgaac ggcgacccca cggttcatct gaacggtatc ggttcgactt
    12781 tgaccgatac gcttgcgggt tcttctgctt ctcacgttga tgcgggtaac caaagtacac
    12841 attacactcg tgcagcaagt attaaggatg tgttgaatgc gggttggaat attaagggtg
    12901 ttaaaactgg ctcaacaact ggtcaatcag aaaatgtcga tttcgtccgc acttacgaca
    12961 cagtcgagtt cttgagcgca gatacgaaaa caacgactgt taatgtggaa agcaaagaca
    13021 acggcaagag aaccgaagtt aaaatcggtg cgaaaacctc tgttatcgct gaagaaaacg
    13081 gtaagttagt tactggcaaa ggcgatgagg gcaaaggctt agttactgca aaaactgtaa
    13141 ttgacgctgt gaacaaaagc ggctggaggg tgaaaacaac tgacaacaat caaacaggtc
    13201 aagctgacaa gtttgaaacc gttacatcag gcacaaatgt aacctttgct agtggtaaag
    13261 gtacaactgc gactgtaagt aaagatgatc aaggcaacat cactgttaag tatgatgtaa
    13321 atgtcggcga tgccctaaac gtcaatcagc tgcaaaacag cggttggaat ttggattcca
    13381 aagcggttgc aggttcttcg ggcaaagtca tcagcggcaa tgtttcgccg agcaagggaa
    13441 agatggatga aaccgtcaac attaatgccg gcaacaacat cgagattacc cgcaacggta
    13501 aaaatatcga catcgccact tcgatgacgc cgcagttttc cagcgtttcg ctcggtgcgg
    13561 gggcggatgc gcccactttg agcgtggatg acgagggcgc gttgaatgtc ggcagcaagg
    13621 ataccaacaa acccgtccgc attaccaatg tcgccccggg cgttaaagag ggggatgtta
    13681 caaacgtcgc acaacttaaa ggcgtggcgc aaaacttgaa caaccgcatc gacaatgtgg
    13741 acggcaacgc gcgtgcgggc atcgcccaag cgattgcaac cgcaggtcta gttcaggcgt
    13801 atctgcccgg caagagtatg atggcgatcg gcggcggcac ttatcgcggc gaagccggtt
    13861 acgccatcgg ctactccagt atttccgacg gcggaaattg gattatcaaa ggcacggctt
    13921 ccggcaattc gcgcggccat ttcggtgctt ccgcatctgt cggttatcag tggtaagggc
    13981 tttttcgcct gtctgctgtt ggggcaagcg gaaggtttga agggaagggt ggcgatttgc
    14041 cgcctgagac ctttgcaaaa ttccccaaaa tcccctaaat tcccaccaag acatttagga
    14101 gatttctcat gagcaccttc ttccagcaaa ccgcccaagc catgattgcc aaacacatcg
    14161 accgcttccc gctattgaag ttggatcaag tgattgactg gcaaccgatc gaacaatacc
    14221 tgaatcgtca a
//
LOCUS       DAWFIE010000053        14205 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWFIE010000053 DAWFIE010000000
VERSION     DAWFIE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14205
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.053"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(92..574)
                     /locus_tag="WGQ71_001417"
     CDS             complement(92..574)
                     /locus_tag="WGQ71_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0707413.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EVRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(727..888)
                     /locus_tag="WGQ71_001418"
     CDS             complement(727..888)
                     /locus_tag="WGQ71_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707414.1"
                     /translation="MSGQPEKHHTSPIEDERKNPVYRMGRAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            complement(962..1423)
                     /gene="recX"
                     /locus_tag="WGQ71_001419"
     CDS             complement(962..1423)
                     /gene="recX"
                     /locus_tag="WGQ71_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ0707415.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(1480..2187)
                     /locus_tag="WGQ71_001420"
     CDS             complement(1480..2187)
                     /locus_tag="WGQ71_001420"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ0707416.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLSNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETA
                     TGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            2594..3859
                     /locus_tag="WGQ71_001421"
     CDS             2594..3859
                     /locus_tag="WGQ71_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ0707417.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDQLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(3917..4723)
                     /locus_tag="WGQ71_001422"
     CDS             complement(3917..4723)
                     /locus_tag="WGQ71_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ0707418.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPIKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            5068..5277
                     /locus_tag="WGQ71_001423"
     CDS             5068..5277
                     /locus_tag="WGQ71_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ0707419.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            5366..6580
                     /locus_tag="WGQ71_001424"
     CDS             5366..6580
                     /locus_tag="WGQ71_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ0707420.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(6667..9246)
                     /gene="clpB"
                     /locus_tag="WGQ71_001425"
     CDS             complement(6667..9246)
                     /gene="clpB"
                     /locus_tag="WGQ71_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ0707421.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHSGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            9506..10516
                     /gene="trpS"
                     /locus_tag="WGQ71_001426"
     CDS             9506..10516
                     /gene="trpS"
                     /locus_tag="WGQ71_001426"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ0707422.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFKEIFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAAFTQMLAEGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            10522..10863
                     /locus_tag="WGQ71_001427"
     CDS             10522..10863
                     /locus_tag="WGQ71_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707423.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNDLEAVSGKCMETDDKGGPVGW
                     AENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            11182..11505
                     /locus_tag="WGQ71_001428"
     CDS             11182..11505
                     /locus_tag="WGQ71_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707424.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(11714..13222)
                     /gene="ppx"
                     /locus_tag="WGQ71_001429"
     CDS             complement(11714..13222)
                     /gene="ppx"
                     /locus_tag="WGQ71_001429"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ0707425.1"
                     /translation="MTTTPANILASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPVGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDSAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMGGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(13338..13586)
                     /locus_tag="WGQ71_001430"
     CDS             complement(13338..13586)
                     /locus_tag="WGQ71_001430"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0707426.1"
                     /translation="MGLRANLRYTRRMFDAPGTIVYRFPRKDHEYQANLSLWHDKISW
                     KGFTPQLNFRYLKIDSNMKSFYTRKNTQIFMSVEKDFK"
     gene            complement(13543..13824)
                     /locus_tag="WGQ71_001431"
                     /pseudo
     CDS             complement(13543..13824)
                     /locus_tag="WGQ71_001431"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
     gene            complement(13823..13990)
                     /locus_tag="WGQ71_001432"
                     /pseudo
     CDS             complement(13823..13990)
                     /locus_tag="WGQ71_001432"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
     gene            complement(13994..14176)
                     /locus_tag="WGQ71_001433"
                     /pseudo
     CDS             complement(13994..14176)
                     /locus_tag="WGQ71_001433"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
BASE COUNT         3206 a         3535 c         3883 g         3581 t
ORIGIN      
        1 aatttaatcc actatatttg cttgatttca tccgtttgca gtagaaacgg atggcgcgat
       61 gtgcctgtct ttcagacggc atagtccgcc gtcagcagcc gcaggacatg tcttcagagg
      121 cttgcaggct gatgtctctg cgttttttgg ctttttcgta gcggcagcgt tggcggtcgg
      181 tcaggatttc cagcgggggg acggggacgg gtttgccgtc tttgaccgca accatcgtga
      241 agtagcaact gttggtatgg cggacttccc ccgtgcggat gttttgtgct tcgacacgga
      301 tgccgatttc catcgaggtg cgtcccgtgt agttgacagc ggcgtaaaag gtaaccaaat
      361 cgccgatgtg gatgggttct ttaaacagga ctttgtcaac cgacagggta acgcaataat
      421 tgccgctgta acggctggcg caggaatagg cgacttggtc gagcaggagc aggagttcgc
      481 cgccgtgtac gttgccgctg aaattggcgg tgtccggcat catcagttcg gacataatga
      541 gttcgtggga aggcagttgg cgttgttgtg tcatggtgta cctgttttaa gaaagggtgt
      601 tttgaaatgg gtgtgggaaa attttaggga atggggaagt ggaattcaag ctaaaaaaag
      661 ctatgatggg atagtctgcc gaaatggacc gccgatgcca tctgaagcct tcagacggca
      721 ttttgtttag gaaagccaaa aacggaagac caggaaaaac acgagtgcca atacggcgac
      781 ccaaacgacg agcatgaatc cggcaactgc ccgtcccata cggtaaaccg ggtttttgcg
      841 ttcgtcttcg atgggggagg tgtggtgttt ttcgggttgt ccggacattt tgttctcaat
      901 cggaaaagtt ggaaacgggc gtaaggttat gttgccgtgc aaaaagattc aagaaggaag
      961 gtcagcagcc gtcttcccaa ttttcgtccc aagcgtgttt caatgccgtc tgaacggtat
     1021 cggcatcgaa tccgcgatag gcgaggaaac gcgcctgttt ttgtttttct ttaaggtcgg
     1081 cggcggggtg tttgaatttt ttacgcaaca cggctatggc ggcctgtttt tcgcttgagc
     1141 ggtcgggaag caggttgcgg ctggtttctt catctatgcc ctgttgcgcc aaagcctgtt
     1201 tcagcctcaa tgaaccgtgt ttgcggcttt tgctgcggat ataggcttcg gcatagcgca
     1261 aatccgactg ccagttgcgt tcggcaaatt cgtttaacac gttttccaac tcctcttcgc
     1321 tttcggcgtg cggtgcaagt ttgcgtttca gaccgatgcg gctgacttct tggcgcgaga
     1381 ggatgtccat cgcgcgggcg cgtagggatt tttgcggttt catggcggcg gtttcggcgt
     1441 gcggcaggtt tcagacggca tagcgtgccg tccgaatgcc tactcttctt tatttttctg
     1501 cggttgcagg ttttcgtaaa tttcgggcag cgagccgata atccagtcgg ggcgggtcgc
     1561 atcgtcttgc gagagcagcg tcatatcgcc gtaaccgaag gtaacgccga cgctcaggca
     1621 gccggcggct ttggcggcga tgatgtcgtt gcgcgagtcg ccgaccataa ccatgtttgc
     1681 aacatcgata cccaaaactt cggcggcgtg ccgcagcggc agggggctgg gttttttctc
     1741 gggcaggctg tcgccgccga gtatcaggct gaagtagtcg gcaagtccga gctgtttcaa
     1801 cagttcgacg gcaaggattt cgtttttatt ggtaatgact gccagcggga tgcccaaaga
     1861 tttgagcaat gccagcccgg ttgcggtttc gggatagggg cgggtgaaga cgctcaaatg
     1921 gtcgcggtag tatttcatat aggatacgaa acctttttcc cacagttcgg aatcggcttc
     1981 gcggtcgcgg tcgttggaga ggacgcggtg aaccagtttg ccgatgccgt cgcccacata
     2041 gctttcgacc actttggcgg gcagcggttt cataccgagt tgttccaaca tcgcttctgc
     2101 ggcggcggca aggtcgggga cggaatcgca cagtgtgccg tccaaatcga aggcgacggc
     2161 ttggacgtgt tcgatggctg cattcataat ctgatttcct gtcgggttga aagggtttat
     2221 tttaacatcg tttgcacagg gcttctaatg tatagattgt taacaatcgc aacaggtttt
     2281 gcaggcggtg ttttcggatt tcggggcgga atcgggcaag ggtttgaaat cccagcgatg
     2341 ttccaattaa atttttaaaa aataaaagga tgcctatatc tttatttaat ttgacattta
     2401 tttttcggat gcgcgcgctg ctttttcagg cggagtctgg gctgattccg tgcggcttga
     2461 aaaataaaat catatataga ttaatcaaat cgggcttatg ccgactacac gctattacat
     2521 tttgtcggga aattggttat agtaatgagc gaaatgcacg cccctgaaaa tctttttgtg
     2581 aaaaggaagc aaaatgtctg aagcccttgc aaaagaaacc ctcaatccgt ttgaaatcgc
     2641 gcgcaaacag gtcaaaaccg cctgcgacca gctgaaaacc gatccggccg tttatgaaat
     2701 cctgaagagt ccgacccgcg tgttggaagt caatttcccc gtcaaactcg acgacggcac
     2761 ggtcaaaacc ttcacgggct accgttcgca acacaacaac gccgtcggcc cctataaagg
     2821 cggcgtgcgc ttccatcccg gtgtgaatct ggacgaagtc aaagccctgt cgatttggat
     2881 gaccatcaaa tgctgcgtag cgggcattcc ttacggcggc ggcaaaggcg gcgttacctt
     2941 ggatccgcgc gattattccg aggcggaact ggaacgcatc gcccgcgctt attccgaagc
     3001 catttctccg ctgatcggcg aaaaaatcga tattcccgcc cccgatgtga acaccaacgg
     3061 caaaatcatg tcttggatgg ttgatgccta tgaaaacgtg gtgaaacatt ctgcaccggg
     3121 cgtatttacc ggtaaaccgg ttgagttcgg cggctctttg gcgcgtaccg aggctaccgg
     3181 ttacggcgtg aacctcgccg ccgtccaagc cttggaaaaa ctgggcaagg acgtgaaagg
     3241 cgcgacttac gccatccaag gcttcggtaa tgtcggttac cacacaggtt attacgcgca
     3301 tcaatccggc gcgaaagttg ttgcggtttc tactgttgac gttgccattt acaacgaaaa
     3361 cggtttggat atggaagccc tgttcaaaga gttccaagaa aaaggcttca tcaccaacga
     3421 agccggttac ggcaaggaaa ttaccaacgc cgaacttttg gctttggatg tggacgtact
     3481 cgccccttgc gcgctggaaa atcaactgac ttctgaaaac gccggtaaag tccgcgcgaa
     3541 aatcgtggtc gaaggcgcga acggcccgac tacgcccgaa gccgacgtta tcctgcgtca
     3601 aaacggcgta ttggtcgtgc ctgatattct ggcgaactgc ggcggcgtgg tcgtttccta
     3661 tttcgagtgg gtgcaaaacc tgcaaggcta ttattgggag tttgacgaag ttcaagagaa
     3721 agaaaccgtc gtcctgcgcc gagcgttccg cgatatttgg aacttggctc aagaatacga
     3781 tgtcgatttg cgaaccgcgt cttatatgat gagtatccgc cgtgttgaaa aagcgatgaa
     3841 actgcgcggt tggtattgat gtttgaacaa aaaatgccgt ctgaagcctt cagacggcat
     3901 ttttgtgcgg gcgggattaa tggtgcgaat ggccgatttg gaaggttaaa gtcgagtagt
     3961 tcgcctgttt ttggcacacg ctttgatcgg ggaagtcggc tttgtgttcg acattggctt
     4021 tccagaagcc ttggcgcaag gggatgatgt ccacttcgcc tttgtcgtct gtgctgtcgg
     4081 agaaagcctg tgcttcggtt ttgtgcgttt tgctgcggtc gctggtgtcg aagccgtcaa
     4141 aggtggcggt aacggtggca ttgggcagcg gttcgccacg gaacagaacg cggactttga
     4201 agcgttcgcc tacgtgaatg ttggcgggat tgtccagcgg gacgatttcc aagttttgtc
     4261 cgaccggttt ggtgatgatg gcggtgtccg cgctttcgtg tccgacgttg acgatgtttt
     4321 tgccgaacat acgggtttgt tcgcaatagc ttgcgtcagg catttctttg atgcccgcct
     4381 gtttccagcc tgctttgttt tttgaccaga aagtaggctg atattcggcg atgacgaggt
     4441 aactgccgtc cttaatggga cggttgcttc ggtactggta gttgtatgtg ccgcgttgaa
     4501 tcatgttttc cttgcctttt tcggtaacca gctgcatcgg tttgctgaag atgtgcaggc
     4561 ggtctttggc gatgggttcg agttcgggaa attcgccgta gcccaagtcg gctttaaggt
     4621 attcgccgcc gtgcgtgtgg gcggtttcga cccagacgcg gtgggcgtgg gcggatgtgg
     4681 caaatagggc ggaaacggcg agcagtgtca atgttttctt catggtgcag cctttgtctc
     4741 gtgtgggtta aagattttgt tatgttataa caaaataggt atcgtgtgaa gacggatttg
     4801 ctgtctgaaa actgtctttt tgtatagtgg attaacaaaa accagtacgg cgttggctcg
     4861 ccttagctca aagagaacga ctctctaagg tgctgaagca ccaagtgaat cggttccgta
     4921 ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatatcg
     4981 atttgaattt ttcagaaaat gaagcggacg tttgggcgga ggcaacttgt ttgataagat
     5041 agcaatattt taaaacggag aaagatcatg ccttatgtca atattaaagt aaccggcggc
     5101 aaggaagcac cgactgccgc gcaaaaagcg gaactgatcg gcggcgtaac cgaattgctg
     5161 gcacgcgtgc tgggcaaaaa tcccgaaaca acggttgtcg tgattgacga agtggatacc
     5221 gataactggg gaataggcgg caaaagcgtc agcgaacggc gcaaagaggg caggtaaaaa
     5281 gcctgaaaat ctcggtttga tgctttaaat tccgcgtgaa aaagagtaca ttcccaccca
     5341 ttgcccaaaa tttacggaac acatcatgga taaatttccc aagtctgcaa agctcgatca
     5401 cgtctgttac gacatacgcg ggccggttca caaaaaagcc cttcagttgg aagaggaggg
     5461 caataaaatc cttaaactca atatcggcaa ccctgcgccg ttcggctttg aagcccctga
     5521 tgaaatcttg gtcgatgtca tccgcaacct gccgacttcg caaggctatt gcgattctaa
     5581 agggctgtat tccgcccgca aagccattgt tcactactat cagaccaaag gtttgcgcga
     5641 tattacggta aacgatgtct atatcggcaa cggcgtgtcc gagctgatta cgatgtctat
     5701 gcaggcattg ctcaacgacg gcgacgaaat cctgattccc gcgcccgact accctttgtg
     5761 gacggcggcg gcaacgcttg cgggcggtac ggtacgccat tatctgtgcg acgaagaaaa
     5821 cggctggttc cccaaccttg ccgatatgga agccaaaatc acgcccaaaa ccaaagccat
     5881 cgtcgtcatc aatcccaata atccgacagg cgcggtgtac agcagggaaa tcctgttgga
     5941 aatcgctgaa ctggcgcgca agcacggttt gattattttc gccgacgaga tttacgataa
     6001 aatcctttat gacggcgcgg ttcaccacca tattgccgcg cttgcccccg accttttgac
     6061 ggtaacgttc aacggtttgt ccaaagccta ccgcgtcgcc ggcttccgcc aaggctggat
     6121 ggtgctcaac gggccgaaac atcacgcaaa aggttacatc gagggtttgg atatgctctc
     6181 gtctatgcgc ctgtgtgcca atacgccgat gcagcacgcg attcagacgg cattgggcgg
     6241 ttatcagagc attaacgaat tcattttgcc cggcgggcgg cttttggaac agcgcaacag
     6301 ggcgtgggaa ctggtcaacc agattcctgg cgtgtcctgc gtcaaaccga tgggtgcgat
     6361 gtatatgttc ccgaaaatcg ataccgaaat gtaccgcatc cgcgatgaca tgaaattcgt
     6421 ttacgatttg ctggtgcgcg aaaaagtctt gctggtacag ggaacggggt ttaattggat
     6481 taaacctgac catttccgca ttgtcacgct gccttacgtc catcagattg aagaggcgat
     6541 gggcaggttg gcaagattcc tgcaaaccta ccgccaataa ggggacggtt tgtctgccga
     6601 ggataaaaaa tgccgtctga aacggagatt cccgtttcag acggcatttt caacagcagg
     6661 aacgaatcag gcaaatttca gtctgtcgcc gtcggcttcc accctgattt cgctttcggg
     6721 cgcatagttt ccggcaagca gggcttttgc cagcgggttt tcgatttccg actggatggc
     6781 gcgtttgagc ggacgtgcgc cgtaaatcgg gtcgaaaccg gctttggcga tgatgtccag
     6841 agcggcatcg gaaacagtca ggcggaggtt ttgtttttcc aaacgttttt ccaagccttt
     6901 gagctggatt ttcgcaatgt tgcggatatt atcctgatcc agtccgtgga acacgaccac
     6961 ttcgtcgatg cggttgatca tttcaggacg gaaatgttct ttcacatcct ccatcacaac
     7021 ttcttttacc gcttcgtaat cctgaatgcc catttgttgg atgtgctggc tgccgatgtt
     7081 ggaagtcatc acaatcacgg tatttttaaa atctaccgtc ctgccttgcc cgtcggtcaa
     7141 acgaccgtca tccaatactt gcagcaggat gttgaacacg tccggatggg ctttttccac
     7201 ttcatccaaa agaatcacgc tgtatggttt gcggcggact tgctcagtca ggtagccgcc
     7261 ttcttcgtag ccgacatagc cgggaggcgc accaattagg cgggcaacag catgtttttc
     7321 catgtattcg gacatatcga tacgaatcag atgatcttcg ctgtcgaaca ggaagcctgc
     7381 caatgccttg cacaattcgg tcttacccac gccggtcggg cccaagaaca ggaagctgcc
     7441 gtagggttta ttcgggtcgg caagaccgga acggctgcgg cggatagcgt cggacacggc
     7501 acgcacggct tcgtcttgac cgactacgcg gcggtgcaac acttcttcca ttttcagcaa
     7561 cttgtcacgt tcgccttcca tcatttttga cacaggaatg ccggtcatac gggaaacaac
     7621 ttctgcgatt tcctctgcgc cgacattatt acgcaagagt ttgtttgccg gttttgtgct
     7681 gtccgtatct gcccgttcgg cggctgcacg ctgtttttcc aaatgctcca aatcttcata
     7741 catcaatttt gaagccagtg ccaaatcgcc ttgccgtttt gcctgttcga ttttaatttt
     7801 gacttcgtca atttgtttct taatattagc agcaccgtct gaaattgctt tttcggcttt
     7861 ccagatttcg tctaaatcgg cgtattcttt ttgcagaccg ttgatttcct cgtctatcag
     7921 ttccaaacgt tttttgctgg catcgtcttt ttctttttca acgtgcgcct tttccatccg
     7981 aagctgaatt agacggcggt cgattttgtc cattgcttcc ggcttggttt ctttttccat
     8041 cttgacacgg ctggcggctt cgtcaatcaa atcaatcgct ttatcgggca ggaagcggtc
     8101 ggtgatgtag cggtcgctca actccgctgc ggcaacgata gcagggtcgg taatatcgat
     8161 accatggtgg atttcataac gctcctgtaa accgcgcaaa atagcgatgg tgtcttccac
     8221 gcttggctcg ccaaccaata ctttttggaa acggcgttca agtgccgcgt ctttttcgat
     8281 gtattggcgg tattcgtcca aggtagtcgc accgatacag tgcagttcgc cgcgtgccaa
     8341 agccggtttc aacatattgc ccgcgtccat cgcgccgtcg gttttacctg cgccaaccaa
     8401 agtatggatt tcgtcgatga aaatcagagt gttgccatcg tctttcgcca agtcgttcaa
     8461 cacgcctttc aagcgttctt caaattcgcc gcgatatttc gcgccggcaa tcaaagccgc
     8521 caagtccaga accagcaggc gtttgttgcg cagggattca ggcacttcgc cgttgacgat
     8581 acgttgcgcc aagccttcaa caatagcggt tttacccaca cccggctcac caatcagcac
     8641 ggggttgttt ttggtacggc gttgcaatac ctgaatcgcg cggcggattt cgtcgtcacg
     8701 gccgataacg gggtcaagtt tgccgtcgcg ggcgcgctgg gtcaggtcaa gcgtatattt
     8761 tttcagcgca tcgcgttggt cttcggcatt ggcatcgttc acgttttgtc ctcctcgtac
     8821 cgcatcgatt gcggcattga tgttttgttc ggtcgcgccg gcttctttca aaattttgcc
     8881 ggccgcatcg ttctgctgca ccaaggcaag caggaaaagt tcactggcga tataggcatc
     8941 gccgcgtttg gttgcggctt tgtccatcag gttcaacacc gcctgcaatt cgcggctggg
     9001 cagaatgtcg ccgccctggc cggacacttt cggcaggctg ttcaaatgct gctgcaaacg
     9061 ctgtttcacc tgcggcacgt tcacgcccga atgagccaag agcgcggcgg ctccgctgtt
     9121 ttggtcgtca agcagggctt ttaacacaaa gcccgcttcc agatagctgc cgtccgcagc
     9181 caacgccaaa ctctgagctt ctgcaagggc ttgttggaat ttggcggtta atttgtcgta
     9241 tcgcattttt gtttcctttt caaaatgtcc gctgtcgaag cctatatgtg cataattgtg
     9301 gataactcaa gttctgtttt ctgtttttct atatttaatt cgatatatca ttgaatttaa
     9361 agtatataaa aatgtataat aatgtgtata actatatctt cttaatatgg aaaagtctgt
     9421 tgtcggctgg atgtaggtgg caaatcgggt ataatcggcg catctttttc cctttcagac
     9481 ggcattgatg ccgcaaggac attttatgag caaaaaacga gttctgaccg gcgtaaccac
     9541 caccggcatc ccgcatctgg gcaactacgt cggtgctatc cgccccgccg tccgcgcggc
     9601 gcaaaaccct gataccgaat ccttcctctt cctcgccgat taccacggta tcatcaaatg
     9661 ccacgagccg gagatgattc accaatccac ccaagccgtt gccgccactt ggcttgcctg
     9721 cggactcgac cccgagcgca ccaccttcta ccgccaaagc gacattcccg aagtgatgga
     9781 attgaactgg attctgacct gcatcaccgc caagggtttg atgaaccgcg cccacgccta
     9841 caaagccgcc gtgcaggcaa atgcggaaaa cgggcaggaa gaccccgatt tcggcgtgga
     9901 aatgggtttg ttcagctatc cgattctgat gaccgccgac atccttatgt tcaacgccaa
     9961 cgaagtgccc gtcgggcgcg accaaatcca acacgtcgaa atggcgcgcg acatcgccgg
    10021 ccgcttcaat caccgcttta aagaaatctt taccctgccc gaagtgaaaa tcgacgaaaa
    10081 cgtcgaactc ttggtcggtt tggacggacg caaaatgtcc aaatcctacg gcaacaccat
    10141 tccgctttgg gaaaacgaca aaaaaaccca aaaatcggtc aacaaaatca tcaccaatat
    10201 gaaagagccg ggcgagccga aacagcccga cgaaagccca ttgtttgaaa tctacaaagc
    10261 cttctccacg ccgtctgaaa ccgcagcgtt cacgcaaatg cttgccgaag gcttggcttg
    10321 gggcgaggca aaaaaacttt tggcggcgaa aatcaacgcc gaactcgccg aaccgcgcga
    10381 acgctacaac gagctgaccg ccgacccttc gcaaatcgaa gagattttgc aggcaggcgc
    10441 gcaaaaagcg cgtaaagaag cacgcgaatt gttggacaaa gtacgcgatg cggtcggcat
    10501 ccgcccgttg aaataaaccc aatgccgtcc gaacctctta aaggcttgcc ggaacaaaac
    10561 gtcgtccggc tgaccggcaa gcatcccaac gacttggaag ccgtcagcgg caaatgcatg
    10621 gaaaccgacg ataagggcgg tccggtaggc tgggcggaaa acggcgtgtg ccataccttg
    10681 tttgccaaac tggtgggcaa tatcgccgaa gacggcggca aactgacgga ttacctgatt
    10741 tcgcattccg ccctgcaacc ctatcaggca ggcaaaagcg gctatgccgc cgtgcagaac
    10801 ggacgctatg tgctggaaat cgacagcgag ggggcgtttt atttccgccg ccgccattat
    10861 tgaggtattc ggacatcccg gaatatattt gggtttttca aaccctgcag gaaaaagtcc
    10921 gcaccgtcgg gaaactcaaa aggaggggga tatgtgttac aattttccga actgttttca
    10981 taaaatagtt ttcggacgtg tttcaatatg gcattgatgc cgccttattg ttcgagaaaa
    11041 aacctttata tttaaatata atgggtttta actaaacggg aaaccgtttt ctctccggcc
    11101 gatgggcaaa atcagccgat tgatggaaca cggtccgatt tttaagcaaa accttaatca
    11161 acaactcaag aaggataaga catgaaaaaa ttattgattg ccgcaatgat ggcggctgcc
    11221 ttggcagctt gttcgcaaga agccaaacag gaggttaagg aagcggttca agccgttgag
    11281 tccgatgtta aagacactgc ggcttctgcc gccgagtctg ccgcttctgc cgtcgaagaa
    11341 gcgaaagacc aagtcaaaga tgctgcggct gatgcaaagg caagtgccga ggaagctgta
    11401 actgaagcca aagaagctgt aactgaagca gctaaagata ctttgaacaa agctgccgac
    11461 gcgactcagg aagcggcaga caaaatgaaa gatgccgcca aataatttgt tgccttggca
    11521 aaaatgatgg gatgccgcct gccggcaacc caaacgaaac cgcctgaaga ttttcaggcg
    11581 gtttttgagt tgtatgcccc ttgtttttta cgcctgcatg accgttccga gcagatgtgc
    11641 cgcgcatatc gggatgttgc ggacaggcaa atgccgtctg gacagggttt ggacggcatt
    11701 tgttccgtaa ggttcagacg gcctcgactt taaacggcat attgattttt tgccattgga
    11761 cgctttcata ttccaatgcg tcggcaatca ggggatggcg ttccagccat tccctgtcaa
    11821 tacgcaggat gaagccgcaa ctttccgtgt ccgtacgcaa ctgcatattt ttcgggaaag
    11881 acaggtcttg gcgcgaacgg cagaacaggg cggcaaggcg cagcgacaaa acggcatacc
    11941 acaacatttc gttggtgccg ataatgccgc ccattttttt catatcgccg cgatgaccga
    12001 tgaccagttg ggcaagtatg gtctgttctt tgcgtgagaa acccggcata tcggcgtttt
    12061 cgaggatgta ggcggaatgc ttgtgatagc cggtgtgggc gatgtccaaa ccgatttcgt
    12121 gcagcgcggc ggcgcgtccg agatactgtt gccacaaggc aagctcttga actgtaacgt
    12181 ttttagcgtg gcagaggctg tccataaagg tttgcgcggt ctcggcggtg cgtttcgcct
    12241 gattgaggct gacgtggtag cggtgttgga actcggcaac cgtttgtccg cgcatatctt
    12301 cgtttaaacc gcgcccgatc aaatcgtaaa acacgccgtc gcgcagggcg gcttcggtta
    12361 cggtcatcct gtcgagtttc atttcctcaa acgccgccat catcacggca agtccgccgg
    12421 caaaaacttc gatgcgttcc ggtttcaggt tttcaaattt ggcttttttg accgaaccgg
    12481 cttcaatgat gcgttcggcg agggcgcgca tgcctttgta ggtaatgtcc gcctcttggg
    12541 gcatttcggc ggcaagcacg tcgcggatgg attttgccga acccgatgtg ccgacggcgg
    12601 aatcccaacc ttcgcgcctc atatttttgc tgatgcgctg gatttcgttg cgggcggcgg
    12661 aaatggcaga ttggaagtct ttggcggtga ttttgttttg gaagaagcgc aggctgtagg
    12721 ttacgcagcc caagggcagg ctttcggtaa tgtcgggatt cagcgtcgaa ccgatgacaa
    12781 attctgtcga accgccgccg atgtcgataa ccagcatttt gccgccgacc ggggggaggg
    12841 tgtggatcac gccggtataa atcagccgcg cctcttcgcg cccggcgatg atttctatgg
    12901 ggaaacccag tgccgcttcg gccttgggaa ggaaatctgc gatgtttttg gcgacgcgga
    12961 atgtattggt tgccacggcg cgcacctgct cgggacggaa gccgcgcagg cgttcgccga
    13021 attttgccag acagtccaaa gcctgttctt gggaagcggc actcaggttt ttctgttcgt
    13081 ccagaccggc ggcgaagcgc accatctgtt tgaacgaatc gatgactttt aattgtccgt
    13141 tgttgttttc gcaaatctgg aggcggaaac tgttggaacc caaatcgacg gaggcgagga
    13201 tgtttgcggg ggtggtggtc atggcggata tcggtggggg aaaaacgcaa tgttactctg
    13261 acggcgcagg cgttgacaat aaatgatgcg gcggtttttg attctgccca cggatgttgc
    13321 cgacggcatt tttgcgctta tttgaaatcc ttttccacgc tcatgaaaat ctgcgtgttt
    13381 ttgcgtgtgt aaaaactttt catattgctg tcgattttca gatagcggaa attgagttgc
    13441 ggcgtaaagc ccttccaaga gattttgtcg tgccacaacg acaggtttgc ctgatattcg
    13501 tggtctttgc gcgggaagcg gtacacaatg gtcccgggtg cgtcaaacat cctgcgggta
    13561 tagcgcaggt ttgcccgcag acccaagccc gccgtcgaac gttttgactg cgccgacacg
    13621 caaacccttg cggacggaag cctgttccgc ctctttcgtc atgttgtgcg accagtccgc
    13681 accgccgtaa agcagccagt ctttcggcgc ggaatacatc agcgtcgcgc ccgccagcgg
    13741 catatggctg tcgtatcggg cggcggtgcg gtcttcctga taacgcttcc acatattgcc
    13801 cacgttcagc gtcagccgcc agttcgctca agcgtcggga gaaatcggca ttgaagccgc
    13861 cgacgaaatt gtatcggctg ccgcctaaga ggttttgctc gataaacggc acgatgccga
    13921 atgagcgcgt tactgaacgg tttttatagc cgagcgacag gcgcaggctc tgttcgctga
    13981 aatctttgtt atcctaatag tgcacgccgc cgccgctgat gtctccgtag aggaaatgat
    14041 gcccgcccgc attgatttcg cgcgacacgc ccaagccgta gcgcaaaccg tgtgcctttt
    14101 gcggcaggct gtcggcggtt ttcgtccagc ttctgcctgc aaattcaatc gttttttcgg
    14161 acgaagcgtt gtttatagcg gattaacaaa aatcaggaca aggcg
//
LOCUS       DAWFIE010000054        13956 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWFIE010000054 DAWFIE010000000
VERSION     DAWFIE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.054"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(86..571)
                     /gene="fxsA"
                     /locus_tag="WGQ71_001434"
     CDS             complement(86..571)
                     /gene="fxsA"
                     /locus_tag="WGQ71_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ0707427.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
     gene            complement(901..1209)
                     /locus_tag="WGQ71_001435"
     CDS             complement(901..1209)
                     /locus_tag="WGQ71_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707428.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
     gene            complement(1193..1495)
                     /locus_tag="WGQ71_001436"
     CDS             complement(1193..1495)
                     /locus_tag="WGQ71_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ0707429.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(1569..3932)
                     /locus_tag="WGQ71_001437"
     CDS             complement(1569..3932)
                     /locus_tag="WGQ71_001437"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ0707430.1"
                     /translation="MQFSYSWLKTQADTELSADKLEHLLTMSGLEVEEAETAAPAFTG
                     VVIAEVKSVEKHPDADRLNVTQVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAMERATELVLQICGGAAGEM
                     VEAQGKLPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAAHGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3968..4486)
                     /locus_tag="WGQ71_001438"
     CDS             complement(3968..4486)
                     /locus_tag="WGQ71_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707431.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGGIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(4493..5557)
                     /locus_tag="WGQ71_001439"
     CDS             complement(4493..5557)
                     /locus_tag="WGQ71_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0707432.1"
                     /translation="MIKSFELEKFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(5734..6726)
                     /gene="pheS"
                     /locus_tag="WGQ71_001440"
     CDS             complement(5734..6726)
                     /gene="pheS"
                     /locus_tag="WGQ71_001440"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0707433.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEVKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(7072..7431)
                     /gene="rplT"
                     /locus_tag="WGQ71_001441"
     CDS             complement(7072..7431)
                     /gene="rplT"
                     /locus_tag="WGQ71_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ0707434.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(7444..7641)
                     /gene="rpmI"
                     /locus_tag="WGQ71_001442"
     CDS             complement(7444..7641)
                     /gene="rpmI"
                     /locus_tag="WGQ71_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ0707435.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(7788..8309)
                     /gene="infC"
                     /locus_tag="WGQ71_001443"
     CDS             complement(7788..8309)
                     /gene="infC"
                     /locus_tag="WGQ71_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ0707436.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(8327..10240)
                     /gene="thrS"
                     /locus_tag="WGQ71_001444"
     CDS             complement(8327..10240)
                     /gene="thrS"
                     /locus_tag="WGQ71_001444"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ0707437.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGVGLARAAVAGRVNGKL
                     VDACDPIVENSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(10294..10370)
                     /locus_tag="WGQ71_001445"
     tRNA            complement(10294..10370)
                     /locus_tag="WGQ71_001445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10334..10336),aa:Val,seq:gac)
     gene            10549..11664
                     /gene="tgt"
                     /locus_tag="WGQ71_001446"
     CDS             10549..11664
                     /gene="tgt"
                     /locus_tag="WGQ71_001446"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ0707438.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQAKKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAIEQGKFADWQARFHENRARGTD"
     gene            11746..12756
                     /locus_tag="WGQ71_001447"
     CDS             11746..12756
                     /locus_tag="WGQ71_001447"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ0707439.1"
                     /translation="MLPFFPEPSLSYTQQNRTAVLLLNLGTPDAPTAQAVRPYLKSFL
                     TDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYEKIWFKEGSPLEVYTARQAAALAER
                     MPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLVIPMYPQYAASSSGAAVDKVCEQLL
                     LQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAEHGRGKKLMLSFHGVPQKHYDLGDP
                     YPDECRHTAKLLAEALELTEDQYVVSFQSQFGRAKWVTPSTQDLFGKLPKQGVTELDV
                     FCPGFLADCLETMEEIALMGREQFYEAGGKSYRYIPCLNDNPDWIDALVALAEENLGG
                     WR"
     gene            12947..13348
                     /locus_tag="WGQ71_001448"
     CDS             12947..13348
                     /locus_tag="WGQ71_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0707440.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(13411..13701)
                     /locus_tag="WGQ71_001449"
     CDS             complement(13411..13701)
                     /locus_tag="WGQ71_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707441.1"
                     /translation="MAKYFTETGISVREYFDFFGEFVVLPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGGGNIGSMGLKVPR"
BASE COUNT         3555 a         3722 c         3074 g         3605 t
ORIGIN      
        1 aacgccgtac tggtttttgt taatccacta tattttcaaa ccggaaaaat gccgtctgaa
       61 ccttcagacg gcattccatt cacatttatt cgtctttttt gtgttcgatg gcgtttcggg
      121 aacgattgcc gccgtaaggc tcttcaaccg tatattcccc ctcgataata tcgtcatcgc
      181 gggaaaagcc ctcttttctg cccgattggt tcatgttgaa aaaattttcc gcacctcctg
      241 cctgcaacac tgcacctccc ttaaacggca gcagcagcaa taccgccaac accgaggata
      301 cgaatcccgg actcatcaga cacaccgccg ccaccgtata acggataggc cacaacatct
      361 gataaacgga taccctcccg ccgcttctca ttgccgcacc cgccaataaa agaccggaca
      421 gccccgtatg cctgagcatc agcacaccgg cggcaaaggt tgccgccatc agaaacagcg
      481 tccaaccgcc gcccagccaa tcggcaaccc acacaatcga cataatctcc aaaaacagca
      541 gcaccaaaaa accgataccg aaaaatctca ttgaccgtca tccttatatt taagtaaaca
      601 gcaaaccgcc cgaacaagac tccaagcgac ctgcctgtaa atgattacaa aaccatgtgc
      661 ttcaagccga aacaatgtga aatcacgcaa tatagtggat taacaaaaac cagtacagcg
      721 ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg
      781 gttccgtact atttgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca
      841 ctataattat tttttagcgt gtaaaacaaa accggctgca tacctgcaac cggcctcaaa
      901 tcagcacaat tccttatcca aatccgccaa cagctctttc agaccgtctc cgatttcctg
      961 aagcgtaacc ggacggttgc cgtccgaacc cgattccgca ccttctgcat acggtgcaaa
     1021 cgtgctctgc aatttcaaca gtttcaccac atccaaacgg gtgtagcgtt cgccgccgta
     1081 accgacaacc acaccgtgat catgctgcca ttgcgcaaaa ccataggggc tgatttgcaa
     1141 cagtccgcac aactcgtcca gcgtgaaata gcgttttgcg ggaaccttca aatcaacgtt
     1201 gtttgtcata gtagtgttcc accatgcttt tgagtttctg gctggcatgg aaagttacca
     1261 cgcggcgggc ggtaatcggc acttcctcgc cggttttagg gttacgaccc gggcgttgcg
     1321 gcttgtcgcg caactggaaa tttccgaaac cggaaatttt gatttcttcg ccgcttgcca
     1381 aagtgctgcg gatttcttca aaaaagagtt cgacgatttc tttggcatcg tttttggtga
     1441 cgttgctgac tttgtctacc aaaatatcgg ccagttctgc tttagtgaga gtcatatgat
     1501 taccttctgt tgtaacagtt gtggattatt accaattttt atttaaaatt caagcgaata
     1561 tttatttttt aactgcgaag ccgcgcccct gccgccgttg ccgcgccaat cagttttccg
     1621 ataagcggct cgaccgcctc atccgtcagc gtgttttcca tatcctgcaa aatcactttg
     1681 accgccacgc tcttcatccc ttcgggcagt cccgtgccgc gatacacgtc aaacacgctg
     1741 atttcctgta ccaacttgtt tgccgcgcct ttcaagacaa gcagcaaatc atcatggctc
     1801 atagcttccg gcatcacaaa cgccaaatcg cggcgcaccg gctggaattt cgatacggcc
     1861 cgatagcgcg ttttcccgca ttccaacacg gccgccatat cgatttcaaa taccagcggc
     1921 gcttgcggca ggtcgtattt ttgcagccat ttcggatgca gttcgccgac aaagccgatg
     1981 actttgccgt ctgaaacgat attggcggca cgtccgggat gcagggcggg atgtccggtt
     2041 ttaacgaact cgactgcttt gtttttcaac agattttcca catctgcctt aatgtcgtaa
     2101 aaatccgcat tgcgcgtttt cccaccccat tgttcgggca ttgccgcgcc gtaccacaga
     2161 ccgccgatgc gttcgttttg gacaaaccgg ccgtctgaac ctttgccgaa cacgcgggcg
     2221 atttcaaaca cgcgcacgcg gttttgtttg cggttcaggt tgttttgcaa aatttccacc
     2281 aagccgccga tgagtgtgga acgcatcacg gcatattgcg ccgccagcgg gttttgcagg
     2341 cggatggggt cggcgttggc ggcaaaatct tgttcccact gctcgtcaac gaaggcatag
     2401 ctgaccactt cgcggtagcc gtgcgccgcc atttcgttgt aaacggcaaa acgcgggcgg
     2461 cgtgtttcgg gcagttccag cattttcaga cggcctgacg tgtaatcgtc ggggatgttt
     2521 tcatagccgt aaacgcgtcc gatttcttca atcaaatcgg cctcaatttc gatgtcaaaa
     2581 cggaagctcg gcgcggtaac gcggaagcct tccgccgttt tctcgggctg caggcccaag
     2641 tgttgcaaaa tggtttccac ctgttcggca ggaatgtcca cgcccaacac ggttttcaga
     2701 cggtccaaac gcagtccgac ctgcttggct tcgggcaatt taccttgtgc ttcaaccatc
     2761 tcgcctgccg caccgccgca aatctgcaac accaattcgg tagcacgttc catggcatcc
     2821 gcctgcaaac ggtaatccac gccgcgctcg aagcggaacg acgaatccga accgaaaccg
     2881 tattggcgcg atttgccggc gatgatttcg ggcgcaaacc aagccgcttc cagcacgata
     2941 ttttgcgtgc cgtctgaaac cgcgctcgcc gcgccgccca ttaagcccgc caaactcaac
     3001 acgccttttt cgtccgccac gaccagcgtg ttttcagaca gggaaacggt tttctcgttc
     3061 aggcattcca gcgtttcccc ttcgcgcgcg cggcggatgt gcaggctgcc ggaaagtttg
     3121 tcggcatcaa aaacgtgcat cggctgaccg atttccagca tcacataatt gccgatgtcc
     3181 accagcgcgg aaatactgcg gatgccgctg cgctccaaac gttgtttcat ccaatcggga
     3241 gtagccgctt tcgcattgac attttcaata acgcggctga taaaacggcc gcaatcggca
     3301 ggcgcgttaa tctgcacggg ctgttttcga ctgcccgtga tcggcgcggt atggatttcg
     3361 ggctgcctga acgcgcaccc cgtcaatgcg gacacttcgc gcgcaatgcc tttgatgctc
     3421 aagcagtcgg cgcggttagg cgtaattttc aacgtaaaca gcgtatcgtc caaatccaag
     3481 tattcgcgga tattggtacc gacgggcgca tcttcaggca gaatgtgcag gccgttcaca
     3541 ccgtcgtcgg gcagaccgag ttcgtcggtg gaacacaaca tcccgtccga caccacgccg
     3601 cgcattttgg tcggcttgat tttgaaatta cccggcaaaa cggcacccgg cagcgaacac
     3661 ggcactttga tgcccgcttt cacattcggc gcaccgcaca cgatctgaac caacccaccc
     3721 gtacccgcat cgacttgggt aacgttcaaa cggtcggcat caggatgttt ttcaacggat
     3781 ttcacttcgg caattaccac acccgtaaac gcaggcgcgg cagtctcggc ttcttccact
     3841 tccaagccgg acatcgttaa caggtgttcc agcttatcgg cggaaagttc ggtatcggct
     3901 tgggttttca gccatgagta ggagaattgc atgttatgtt ctcaattaac taattaactt
     3961 tagattttca agagctagct aaaccttttt gaatggctaa gtccctatta attacttcta
     4021 cagaaatgtt ccataaagaa atcaaccgca aagtatcttt cttattaaat tcttgcttta
     4081 ttaattcatt caattgccca agataattaa gcggcacatc cgggataacc agtaataatt
     4141 tgatttctat gccaccccat tgcaaatttg aaaaagcact aggcataatt gtttctttga
     4201 tatcttctct gcctaatatt cccaaaattt caacatttaa tgcattacac catttttgat
     4261 aaatctctga aatatattca tcaaaacgct caggagatct ttcttgattt ggaatacttt
     4321 gttttgcctc aacaaataac aaattatctt cttgcagata gataaactct actgtcttaa
     4381 taccttcttg ccttctcttc cggatatatg gttctatata aaacaggtta ttttttacta
     4441 gattaaaagt tatacctgat tcagtgagat ttattatttc actcataaaa acctaaccaa
     4501 tccatgctgt ttccggagat aaaacaccta tttcttctct gaacaatcga atagactcac
     4561 caataatttc attatccggc atgctatttt gtaaattttc ctgcacccaa gtagtctgtt
     4621 ccttttcttt gccaggcata aaaacaggaa tactttcact atgctttcta gctaataaac
     4681 acagcttctt gataacaaaa tatgaatgcg tactcataaa aatctgtatt cccaatttgg
     4741 atttagataa ggaaaacaga atttctagaa attgattaat tgctttcgga tgtagattag
     4801 cttctggctc atcaataaat aagattgatc caggactcaa aaaccgattt cctaatagag
     4861 tgtctaatat cccaatcttt tttaccccct ctgctgtaga attgatagag aaagtttgtc
     4921 tatctttttc atatacccat gccttctttt tatcattgta gctaacataa ccgccaaaca
     4981 atgcttctag atcctttcta gcttccgctg cctcagggta attcctccct tttgttggtt
     5041 taatatccaa tgcttttacc aaatcaatgt aagtagcatc aaaaccaaat ctcttttctt
     5101 cctctgattt tttaattaca tcaaatagtg atagaacttc tttgggtggt aaaaaaatag
     5161 agttagcgtt tattctggga gaaagttcgt atagctccgg tttaattagc tttttggtag
     5221 ttctaccaaa agaaaacaaa actgaagaac tatcttctaa agtcatactt aacttaagag
     5281 gccgttcgtt tggattttct tttcctcttg taactaaatc accaatttcc tctacttgga
     5341 acgtccaata tagcttatct cgcaattcct cagaaaaatc acgaatattc tttcctttat
     5401 gagcttcctc atggcttttt aaaacactat ataatgcctt taaaataaaa gttttccctt
     5461 tgctattttc tgcaataatt aaatttattt tccctaaatt atcagccttc aataagctaa
     5521 tcggtccgaa tttttcaagc tcaaatgatt taatcatata atccccctaa tatatatcaa
     5581 caatatttgt acaagccaca ttgtaaaaag acagcaacca cagcaccata gtaaacgcat
     5641 agcaggcgga taatggagcg acatagcgtt tcatgattta gctttctgag gtaggcacag
     5701 gctgcttttt tcatttttag gcagcctaca caatcatttt acaaactgct tcaaaaaatt
     5761 caaatcgtta tcaaaaaaca agcgcaaatc gttcacgttg taacgcagca tcgcgaaacg
     5821 gtcgagaccg ataccgaaag cgaaaccggt gtatttttca gaatcgatat tcacattttt
     5881 caacacgttg ggatgcacca tgccgcagcc gccgacttcc agccatttgc cgttttcgcc
     5941 cataatgtcg atttcggcag acggttcggt gaacgggaag aaagacgggc ggaaacgtac
     6001 ttgcaaatca tcgcgttcaa agaagcgacg gataaaatcc gtgaacactg ctttcaaatc
     6061 ggcaaaagtt acgccctctt ctacccacaa accttccgcc tgatggaaca taggcgagtg
     6121 cgtggcatcg ctgtccacac ggtaaacgcg gccgggggcg ataatgcgga tgggcggctc
     6181 ttttttatcg agcatatagc ggatttgaat cggggaagtg tgcgtacgca aaacatcgcc
     6241 gttttcaacg taaaacgtat cctgcatcgc acgggcagga tggtttgcag ggatgttcag
     6301 ggcttggaaa ttgtgaaaat cgtcttcgat ttcaggcccg tccgccactt cgaaacccat
     6361 tccgtgaaag agttcgacca cacgttgcaa ggtcagggtt acgggatgca ggctgccgcc
     6421 ttcctgagcg cgtccgggca gggtaatatc gagggcttcg gcggcaagtc gggcttgcag
     6481 cttgacttcg ttgagggcat cgcgtttggc attaaaagcc gtctgaaacc ggtttttgca
     6541 ttcattgata tgcgcaccta tggttttgcg ctcttcaggc gacatttgcc ccaaagtttt
     6601 cagaagtccg gtcaactcgc cggttttgcc aagataacgg gctttgattt gttctagagc
     6661 gttgaagtct tgcgcagctt ctactgcggc aatgccttct gcaacgatgc ggtttacatt
     6721 ttccataata tcaagtctgt caattaatga tatgccacct gaaacaacga ggccgcctgt
     6781 atagcctgac atatcagact cttcagacga ccttattttc ttcacggtca atccattgga
     6841 taaattttct gtatagtgga ttaaatttaa accagtacgg cgttgcctcg ccttgccgta
     6901 ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt taaatttaat ccactatatt
     6961 ttaacgcaaa tttatttaaa aaaatagact ttaaagaaag aaaatcaatc acatagaatt
     7021 tctgcaaaca aagaaaaaag gaagccgcag cttccttttc aattttttgg attaagcagc
     7081 caaagcagct ttggcttttt caaccaattg tgcaaaagcg gctttatcga acacggccaa
     7141 atcagccaat actttgcggt cgatttcgat agaggcgcgt ttcagaccat tcataaattt
     7201 gctgtaagac aacccgtttt cacgcgcacc tgcattgata cggacaatcc acaattgacg
     7261 gaattggcgt ttgcgttggc ggcggtcacg gtatgcgtat tgaccggctt tcattaccgc
     7321 ctgcttggca acgcggtaaa cgtttttacg acgaccgcga tagcctttgg ctaacgcgaa
     7381 gattttttgg tgacgggcac gggcggtaac accgcgtttt acgcgtggca tattctaaac
     7441 tccttaagcg tagggtaaca ttttagcaac agaagccaaa tcgcgatcgt ttaccataga
     7501 ggtaccgcgc agttggcgtt tgtttttggt ggtcttttta gtcaagatgt ggcgtttgaa
     7561 cgcatgagcg cgtttcacac cgccgttacc cagtacttta aagcgttttt tcgcgctaga
     7621 cttggttttc attttaggca tgggaaaact ccattcgtta tcggataagg cattaggggg
     7681 ttttaaaaca tcggtttcaa acacttgaac ccacaatacc acggtttttt gccgcaattg
     7741 aaaacttact ccgaagcggc aatcggagta agcggagaat tatagcttta ttttttcttc
     7801 ggcgcaatca tcattaccat ttggcgacct tccattttgg gaaaggactc gatttgcgcc
     7861 acttcagcca aatcttcttt tacacgttcc aaaagttgcg cgccgagttg ctggtgagcc
     7921 atttcacgac cgcggaaacg caatgtcact ttgactttat cgccgtcggc aaggaagcgg
     7981 ttgatgttgc gcatcttgat ttgataatcg ccctcatctg tacccggacg gaatttaatt
     8041 tccttgattt gtacctgctt ttgatttttc ttggcttcgt cgcgcttctt ggcttgttgg
     8101 tatttgtatt taccgtaatc catcagtttg catacaggcg gtttagcagt tggggaaatc
     8161 tctaccaaat cgacatcctg cccttcggcc atagccaaag cttcacgaac tgaaacgaca
     8221 ccaagctgtt cgcctgactc actgattaaa cgcacttctt tggcggtaat ttcgccgttg
     8281 attcgtgctt cgcgttcttg agcgatgatg aatactccta taaaaattaa tgattgacga
     8341 gggcatcagt gatttcttgc tgcaattgcg caatgaaatc atccaaatcc aaagaaccca
     8401 aatcttctgc tttgcggcgt accgccactt tgttttcctg cttctcctta tcgccgacaa
     8461 cgatttgata agggaaacgg tattggctgt tgtcgcggat tttgtaaccg attttttcgt
     8521 tacgcaaatc caactcggcg cggaatcctg ccgcctgcaa tttggcagcc acttcccgac
     8581 aataatctgc ctgattttcg gtaatattca taattaccaa ttgaaccgga gccaaccata
     8641 acgggaatga gcctgcatgg ttctcaatca gaatgccgat aaaccgctcc aaagaaccta
     8701 aaatggcgcg atgcaacata acaggacgcg cacggtcgtt gttttcagtt acatattcgg
     8761 cattcaaacg ctccggcaat acgaagtcca attgcagggt accgcactgc caagagcgac
     8821 ccaaggcatc tttgacatga tattcgattt taggcccgta aaacgcaccc tcgcccggca
     8881 attcgcccca ttccacgccg caggcagtca atgcctcgcg caaaccctgc tctgctttat
     8941 cccacacgtc atctgaacct gcgcgttttt cagggcgaag agaaagcttg acggatacat
     9001 catggaaacc gaactgtttg tagatgcgaa ccaacaattc attgaacgca cgggcctcgc
     9061 tgacgatttg gtcttcggta cagaaaatat gcgcatcatc ctgcacaaaa ccgcgaaccc
     9121 gcatcagacc gtgcagcgca ccgctcggct cgttgcggtg gcaagaaccg aattccgcca
     9181 aacgcatcgg caaatctcga tacgaacgca aaccgttgtt aaaaatttga acatgacccg
     9241 gacagttcat cggtttaacc gcatattcgc gtttttccga actggttacg aacatattgt
     9301 ctttgtagtt gtcccaatgg ccggattttt cccaaaaggt tttatccata atttggggcg
     9361 ttttgacttc tttataaccg gcggcattca gctctttccg catatgctgc tcaatcactt
     9421 gccacaaagc ccagcctttg ggatgccaaa acaccatacc cggtgcttcg tcttgcaggt
     9481 ggaacaaatc caattgcttg cccaatttgc ggtggtcgcg cttttcggct tcttcgatac
     9541 gttgaatata ggctttcaac tcgtcttttg tcgcccaagc cgtaccgtat atgcgttgca
     9601 gcatttcatt gttgctgtcg ccgcgccagt atgcgcccgc caacttggtc agtttgaagt
     9661 ttttcaggaa acgggtattc ggaacgtgcg gtccgcggca catatcgaca tattcctgat
     9721 gatgatacat ccccatcgct tccacttcgg gcatatcgtc aatcaggcgc agtttgtatt
     9781 cttcgccgcg ctcttgaaaa attttaatcg cctccgcacg cggagtcatg attttgacca
     9841 catcatagtc ttgggcaatc aattctttca tacgcgcttc aatggcggca acatcttccg
     9901 gtgtaaacgg tttttccgtg gcgatgtcgt aataaaagcc ctcttcaatg acggggccga
     9961 taaccatttt tgcattagga tagagttgct tgacggcatg cccgacaaga tgggcgcagg
    10021 aatggcggat gatttcgatg ccttcctgat ctttcggagt aatgatttga acagcagaat
    10081 tttcaacaat cgggtcgcac gcatcgacca atttgccgtt taccctgcct gccaccgccg
    10141 ccctcgccaa accgacaccg atagacgcag caatttgagc cacggtaacg ggggattcgt
    10201 attggcggac tgagccgtcc ggcaaggtaa tgttcaacat caaaaagctc caaaacatta
    10261 aaaaataacg gcataaatgc cgttatggga atttggtagg cacgattgga ttcgaaccaa
    10321 cgacccccac catgtcaagg tggtgctcta accaactgag ctacgtgcct tcaaagaatt
    10381 tttgcatttt atcgggtcgt tttaattttt gcaagaggta tgtgcatttc tttatgcaac
    10441 attgtggcaa gatttgggga tgtcgtctga aaaaccgtgg gaattcattt ataatgccgt
    10501 cttttaaaac atatttcgcc ccctgtcgcc caactgacat tcaatcttat gcttaaattt
    10561 accttacaca aaaaagacgg atacgcccga cgcggcacgc tggagttgaa ccacggcaaa
    10621 attgaaacgc cggtatttat gcctgtcggt acttacggct cggttaaggc gatgaacccg
    10681 caaaacctgc acgacatcaa ggcgcaaatt attttgggca acacttatca tttgtggctg
    10741 cgtccgggct tggaggtcgt cgaacagttt ggagggcttc acggctttat cggctgggac
    10801 aaaccgattc tgaccgactc gggcggtttt caggtttttt ctttgtcgga tatgcgcaag
    10861 ctgacggaag aaggctgtac gttcaaaagc ccgatcaacg gcgacaagct gtttttatcg
    10921 cctgaaattt ccatgaaaat ccaaacggtt ttgaactcgg acatcgcgat gcagctggac
    10981 gaatgcacgc cgggcgaagc aacgcgcgaa caggcgaaaa aatcgctgca aatgagcctg
    11041 cgctgggcgg aacggagcaa aaaagccttt gaagatttga aaaacccgaa cgcgctgttc
    11101 ggcatcgtgc aaggcgcgat gtatgaggat ttgcgcgaag aatcgctgcg cggtttggaa
    11161 cagtttgatt ttcccggcct tgccgtcggc ggtttgtccg tcggcgaacc caagcccgaa
    11221 atgtaccgta tgctgcgcgc cgtcggtccg attctgccgg aacacaagcc gcattacctg
    11281 atgggcgtag gcacgccgga agacctcgtg tacggcgtgg cacacggcat cgatatgttc
    11341 gactgcgtga tgcccacccg caatgcacgc aacggctggc tgtttacccg tttcggcgat
    11401 ttgaaaatca aaaacgccaa acacaagctc gacaagcgtc cgatagacga aagctgcacc
    11461 tgctacgcct gccaaaactt cagccgcgcc tacctgcacc atctgcaccg cgccggcgaa
    11521 atcttgggcg cacagctcaa caccatccac aacctgcatt tctaccaagt cattatggcg
    11581 gaaatgcgcg atgccataga acaaggcaaa tttgccgact ggcaggcgcg attccacgaa
    11641 aaccgtgctc gcggcacaga ctgactcccg gcaatgccgt ctgaagccgc ttcagacggc
    11701 attttgccgc tttaccctac ccgcactatt tttcagaaac cgatcatgct tccattcttt
    11761 ccagaaccct ccctttccta tacgcaacaa aaccgtaccg ccgttttgct gttgaacctc
    11821 ggcacgcccg acgcgccgac cgcgcaggcg gtacgacctt atctgaagtc ctttctgacc
    11881 gaccggcgcg tcgtcgaact gcccaaatgg ctgtggtatc ccattctgca tggtctggta
    11941 ctgacgttgc gccccaaaaa aagcgcgcac gcctatgaaa aaatctggtt taaggaaggt
    12001 tcgcccctag aagtttacac agcacgccaa gctgccgcgc ttgccgaacg tatgcccgac
    12061 ctcatcgtcc gccacgccat gacctacggc aatccttcgg tggcagacgt attgtcggaa
    12121 ctgaaggcgc aaggcgcggg cagactgctg gtcatcccga tgtacccgca atatgccgct
    12181 tccagcagcg gagcggcagt agataaggtg tgcgaacaat tactgctgca acggaaccaa
    12241 atgagcgtcc gcaccgtttc gcgcttttat gacgataccg gctacattga tgcgatgaaa
    12301 aaccacatcc tccgatattg ggcggaacac ggacgtggga aaaaactgat gttgagtttt
    12361 cacggcgtac cgcagaagca ctacgacctc ggcgacccct atcccgacga gtgccgccac
    12421 accgccaaac tgcttgccga agcactggaa ctgaccgaag accaatatgt cgtatcgttc
    12481 caaagccaat tcggcagggc aaaatgggtt acaccgagca cgcaggattt gttcggcaaa
    12541 ctgcccaaac agggggtaac cgagctggac gtattctgcc cgggcttttt ggcagactgt
    12601 ttggaaacca tggaagaaat cgccctgatg gggcgtgaac agttttacga agcaggcggc
    12661 aaaagctacc gctacatccc ctgcctcaac gacaaccccg actggataga cgcactcgtc
    12721 gcacttgccg aagaaaacct tggcggctgg cgttgaaact gtttgaccca tgtctaaaat
    12781 gccgtctgaa cgccgtgttc agacggcatt ttcccgctac tttagcgtat gatggcatct
    12841 atgccggaca gcacatcaag tgcaacctta ccaccgattc ggcaaaaaaa cctttcaccc
    12901 gctacgcaaa atcaacaaca aggtttcaaa cattaaggaa acaccaatga acacaacccg
    12961 actgccgacc gccctcgtct tgggctgcct ctgcgccgcc gcttctgccg ccgacaacag
    13021 catcatgaca aaagggcaaa aagtgtacga atccaactgc gtcgcctgcc acggcaaaaa
    13081 gggcgaaggc cgcggaacca tgtttccgcc gctctaccgc tccgacttca tcatgaaaaa
    13141 accgcaggtg ctgctgcaca gcatggtcaa aggcatcaac ggtacaatca aagtcaacgg
    13201 caaaacctac aacggattca tgcccgcaac cgccatcagc gatgcggaca ttgccgccgt
    13261 cgccacttat atcatgaacg cctttgacaa cggcggcgga agcgttaccg aaaaagacgt
    13321 aaaacaggca aaaggcaaaa aaaactaaac agacaaaatg ccgtctgaaa ccggcaatcc
    13381 ggcttcagac ggcattcaaa tcaaaccttt ctaacgcggt actttcagcc ccatagaccc
    13441 gatattgccg ccaccgtcaa cctcctttgc ggtcagcata aagccgccga tgtcaatacg
    13501 gccgcactcg accggctcct gactgtcgga acgcttatcg aaaagctcgg caaggctcaa
    13561 accctcttcg cccgcttcca gcctcaaacc gtaagcaagt gccaaatcac ccgaacgtgc
    13621 tgccggcaaa acgacaaact caccgaagaa atcaaaatac tcacggacgc taatacccgt
    13681 ctcggtaaag tatttcgcca ttttatagtg aactaaattt aaaccagtac ggcgttgcct
    13741 cgccttagct caaagagaac gattctctaa ggtgctgaag caccaagtga atcggttccg
    13801 tactatttgt actgtctgcg gctcgccgcc ttgtcctgat ttaaatttaa ttcactatat
    13861 cgaccttgcc gtcgggcagg atgtaccacg ccaaatcccc gcctgaagca cggtgtcgga
    13921 ctgcatttct attcggctat acgccttttc acgcag
//
LOCUS       DAWFIE010000055        13829 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWFIE010000055 DAWFIE010000000
VERSION     DAWFIE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.055"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..79)
                     /locus_tag="WGQ71_001450"
     CDS             complement(<1..79)
                     /locus_tag="WGQ71_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0707442.1"
                     /translation="MKPVVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="WGQ71_001451"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="WGQ71_001451"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ0707443.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKKPGLIIGVAGCVASQEGENIVKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYCGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPNMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1695..3602)
                     /gene="dxs"
                     /locus_tag="WGQ71_001452"
     CDS             complement(1695..3602)
                     /gene="dxs"
                     /locus_tag="WGQ71_001452"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ0707444.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRTFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSENKPAAKPTYTQVF
                     GKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLACE
                     GMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSFLR
                     CIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGVPVSDGMETVEIGKGII
                     RREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVTLE
                     ENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVERRV
                     RAWLSDRDAAN"
     gene            complement(3687..4604)
                     /gene="xerC"
                     /locus_tag="WGQ71_001453"
     CDS             complement(3687..4604)
                     /gene="xerC"
                     /locus_tag="WGQ71_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ0707445.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEAEGGVPQGLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4798..5862
                     /locus_tag="WGQ71_001454"
     CDS             4798..5862
                     /locus_tag="WGQ71_001454"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ0707446.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVGAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            5882..6841
                     /locus_tag="WGQ71_001455"
     CDS             5882..6841
                     /locus_tag="WGQ71_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ0707447.1"
                     /translation="MPSEPLFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTAPLDHKDKSLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGE
                     FQIYKQDHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAFNQLPDGKAEYHG
                     KAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPEQNVELAAAELKADEKSHAVILGDT
                     RYGSEEKGTYHLALFGDRAQEIAGSATVKIGEKVHEIGIAGKQ"
     gene            6980..7657
                     /gene="tsaB"
                     /locus_tag="WGQ71_001456"
     CDS             6980..7657
                     /gene="tsaB"
                     /locus_tag="WGQ71_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ0707448.1"
                     /translation="MQADFNRPVLAIDTGTSYLSLALRADGETRLFHQEAGSRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSGYQVGRAADIRLPEGCAFSDG
                     IGNAFALENRPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERRARL"
     gene            7654..8094
                     /gene="rimI"
                     /locus_tag="WGQ71_001457"
     CDS             7654..8094
                     /gene="rimI"
                     /locus_tag="WGQ71_001457"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ0707449.1"
                     /translation="MNIRRAVYADCAALAALDAVCNPSAWTQRQFESALVSLSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFTHLPEDTQRLLLE
                     VRAGNTAAQALYAAHGFSIAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8088..8831
                     /locus_tag="WGQ71_001458"
     CDS             8088..8831
                     /locus_tag="WGQ71_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ0707450.1"
                     /translation="MLSARYLHLHEALGLGPMWLKRGAVVLPSATLPESPTQIRPQKQ
                     TVLSIPQRPSEQHTGQARLETMKALETAAVPTRKPAPETETPLSGVSDGIAPVSAASG
                     ITKLAVVSLCPPTEDMVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNP
                     MPSEQAIANALGQIARELDSCRAPAVLFLGQAFVNPERQAMIETLCGSRPFFIIDHPA
                     RLLRQPELKARAWQVLKQLKRALRQGGGS"
     gene            8893..9534
                     /gene="pyrE"
                     /locus_tag="WGQ71_001459"
     CDS             8893..9534
                     /gene="pyrE"
                     /locus_tag="WGQ71_001459"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ0707451.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRQSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9608..10042
                     /locus_tag="WGQ71_001460"
     CDS             9608..10042
                     /locus_tag="WGQ71_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707452.1"
                     /translation="MPACFCPHCKTRLWVKENQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTLPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10039..11349
                     /gene="argA"
                     /locus_tag="WGQ71_001461"
     CDS             10039..11349
                     /gene="argA"
                     /locus_tag="WGQ71_001461"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ0707453.1"
                     /translation="MSAPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVLLISGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYGGKTLHLDMLQTAASAAVSLQAEKLVYLTLSDGIARPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            11511..13526
                     /locus_tag="WGQ71_001462"
     CDS             11511..13526
                     /locus_tag="WGQ71_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ0707454.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAKANAETRTRFLENDKARALS
                     DGILAQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIAEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPADCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFTDGKWQEAELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFVSDGINVQQFWTASSDGERIPYFHVGKNAAPDMPTLVYAYG
                     GFGIPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDDVPYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQREAADELACVLLFLKEFLG"
     gene            complement(13618..>13829)
                     /locus_tag="WGQ71_001463"
     CDS             complement(13618..>13829)
                     /locus_tag="WGQ71_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEZ0707455.1"
                     /translation="RPYHSWEKGLNENTNGLIRQYFPKQTDFRNISNREIRRVQDELN
                     HRPRKTLGYETPSVLFLNLFQPLIH"
BASE COUNT         3104 a         4248 c         3698 g         2779 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccaaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccatacaac cggtttcata cacatctccc attaaagcca aacattatac agccgtccaa
      121 accgattaaa ttcatatttt aaaacaatat cctgcctcca aaacccacat cgtgctataa
      181 tccacaccga ttttcagacg gcatcgtcat gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gcaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcgcaag aaaaagtctt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaaa cccggcctca tcatcggcgt tgccggttgc gtcgcctcgc
      541 aagaaggcga aaacatcgtc aaacgcgcgc cgtatgtgga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgcccaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt tcccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca
      841 accttgccca gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatt
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aattggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgtgccatcc gtcctgattt atgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acattgcctt cgacttgagc ttcgtgttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgca cgacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaacgat gatcggcacg gttcaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccagct ccaagcccgc accgccaaca
     1501 accgcgtcgt caactttacc ggcacgccca acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaagttgt cgaagcctaa acccttacgc
     1621 cgaaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgatgg
     1681 cgcggccagg cggcttaatt tgccgcatcc cgatccgaca accacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacaaagcgc
     1981 atatcggcga cggtggcgtt cagtttttcg gcaaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaaggcggt tttctcacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcacg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg cgtctgcctg atagcaggtc gaaagcagca ggcggcattc attttcatcg
     2221 ctcggcgcgg cgacaatcat attcggaatg cagcgcaaaa agcttaaatc gtacaaaccg
     2281 gcatgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggc
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catcccttcg caagccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg caatgccgac gtcgaaatag cggtcgggga atcgttgttc aaactcaacc
     2521 aagccgctgc cctcgcgcat ggcgggggta atcgcaacca gtcgggaatc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcggc aggtttgttt
     2641 tcagacggca tttgcgccgc gctttctttg ggcaggttgg cgacggcgtg gtatttgacg
     2701 gggtcgtttt cggcgagttt gtagccgttg ccctttttgg tgatgacgtg cagcagttga
     2761 gggcctttgc ggctgcgcaa gtctttcaat acgtccacca gattttcgac gttgtgtccg
     2821 tccacggggc cggtgtagcg gaagccgaag ttttcaaaca aagacagcga ctgtttggcg
     2881 tgttcggctt cttcggcaag ggttttgatt ttgtgttcga ctttttgggc aaactccatc
     2941 gcgccgggta ttttgtctaa taccttgccc gtttgcgctt tgacggtact caacaggccg
     3001 tgcatatcgc gcacgacgtt gctggcaagg tatttcggca gcgcgccgac gttgggggaa
     3061 atcgacattt cgttgtcgtt gaggacgacc agcaaatcca catccatatc gcctgcgcaa
     3121 ttcaaggctt caaacgcctg ccccgccgtc atcgcgccgt cgccgatgat ggcgacgctg
     3181 cggcggtcgc tgcccaagag tttgtctgcc gccgccatgc ccaacgccgc gccgatggag
     3241 gtggaggaat gccccacgcc gaacgcgtcg tactcggact cgcaacgttt cggaaaaccc
     3301 gccaaaccgc catattggcg catggtgtgc atctggtttt tcctgcctgt caggattttg
     3361 tgcggatagc tttggtgtcc gacatcccac accagcttgt cttcgggcgt gtcgtacaca
     3421 tagtgcaggg cgatggtcag ttcgaccgcg cccaaattgc tggcgaaatg cccgccggtc
     3481 tgcccgacag attccagcag aaaggtgcgc aactcgccgg caaggcgcgg cagctgtttt
     3541 ttgtccagac ggcgcaaatc ttgcgggctg tcaatcaggt cgagtagggg gcttgggttc
     3601 atggtgtgtc ttttttatgt gtcgtccagg tgcaacgacc aattatatat caagagcgtg
     3661 cggctgacgg ctgattttgc cgtatgtcat tcgtcctgcc gcttggcgcg cgggtgggct
     3721 tcgtcataca ggcgggcgat gtggtcgaaa tcgagtttgg tgtaaatctg cgtggtcgaa
     3781 aggctgctgt gcccgagcag ctcctgcacc gcccggatgt cgcgcgaagc ctgcaacagg
     3841 tgtccggcgt agctgtggcg catcatatgc ggcgaaacgt gcctgccgtc gccgttttgc
     3901 gccgcccatt gcgccaaacg tttttggatt tggcgttggc tcaggcgcgt gccgttcctg
     3961 ccggtaaaca gggctttgcc gtccgatgcc gtctgacgca gcggcagata gtttttcagg
     4021 gcttccacgc ttttgccggt cagcggcacc tgccgctgct tgcgcccttt gccggtaacg
     4081 tgtacccacg cctcgtccaa atagacatca tctgcattca agccgtgtat ctcgctcacg
     4141 cgcaaaccgc tgccgtacat cagctcgaac agggcgtgat cgcgcaccgc cagcgggtcg
     4201 ccgccgtcca cgggcaaatc cagcatccgg ttcagccatt cctgcggcag ggctttgggt
     4261 acgcgctcgg gctgcttcgg cggtttgatg tcggcggtcg ggtcggtatg cagcagaccg
     4321 cgttccacca gccaaacaca atactgccgc caagacgaca gcttgcgcgc cagcgtccgc
     4381 gcattcaaac cgcgctgcga cagccgccgc aacgccgccg taaaatcgcg ccgcgacaag
     4441 ccctgcggca cgccgccttc agcttcagac ggcatttgtg ccaacagcgc aaacagttct
     4501 tccaaatcgc gccggtatgc cgccaccgtg tgctccgact tgccctcgcg cacgatattt
     4561 tccaaataag cgtcaaaatg cgccgcaaac ccgtccaaaa ccataaccgc cccacacaaa
     4621 tatcaaaaaa acagtgaaaa cattatcata aacgacacac caaaaccccg aactgcccaa
     4681 aatccgaccc gtccgcccgc cgttatcgtt aacataaaat caatgagccg tttatggttt
     4741 tttgccgcaa aaaacattat aatccgcctt atttacctat tgcccaagga gacacaaatg
     4801 gcactcgtat ccatgcgcca actgcttgat catgctgccg aaaacagcta cggcctgccc
     4861 gcgttcaacg tcaacaacct cgaacaaatg cgcgccatta tggaagccgc cgaccaagtc
     4921 ggcgcgcccg tcatcgtaca ggcgagtgca ggtgcgcgca aatatgcggg cgcgccgttt
     4981 ttgcgccacc tgattctggc ggcagtcgaa gaatttccgc acatccccgt cgtgatgcac
     5041 caagaccacg gcgcatcgcc cgacgtgtgc caacgctcca tccaactggg cttttcctcc
     5101 gtgatgatgg acggttcgct gatggaagac ggcaaaaccc cttcttctta tgaatacaac
     5161 gtcaacgcca cccgtaccgt ggttaatttc tcccacgctt gcggcgtatc cgtcgaaggc
     5221 gaaatcggcg tgttgggcaa cctcgaaact ggcgaagccg gcgaagaaga cggtgtaggc
     5281 gcagtgggca aactttccca cgaccaaatg ctcaccagcg tcgaagatgc cgtgcgtttc
     5341 gttaaagata ccggcgttga cgcattggcg attgccgtcg gcaccagcca cggcgcgtac
     5401 aaattcaccc gtccgcccac aggcgacgta ttgcgtatcg accgcatcaa agaaatccac
     5461 caagccctgc ccaatacaca catcgtgatg cacggctcca gctccgttcc gcaagaatgg
     5521 ctgaaagtca ttaacgaata cggcggcaat atcggcgaaa cctacggcgt gccggttgaa
     5581 gaaatcgtcg aaggcatcaa acacggcgtg cgtaaagtca acatcgatac cgacttgcgc
     5641 cttgcttcca ccggcgcggt acgccgctac cttgccgaaa acccgtccga cttcgacccg
     5701 cgcaaatacc tgagcaaaac cattgaagcg atgaagcaaa tctgcctcga ccgctacctc
     5761 gcgttcggtt gcgaaggtca ggcaggcaaa atcaaaccgg tttccttgga aaaaatggca
     5821 aaccgttatg ccaagggcga attgaaccaa atcgtcaaat aacaggttgc ttgtaaacaa
     5881 aatgccgtct gaaccgttgt tcggacggca tttgattttt gcttctttga cctgcctcat
     5941 tgatgcagta tgcaaaaaaa gataccataa ccaaaatgtt tatatattat ctattctgcg
     6001 tatgactagg agcaaacctg tgaaccgaac tgccttctgc tgcctttctt tgaccgccgc
     6061 cctgattctg accgcctgca gcagcggagg cggcggtgtc gccgccgaca tcggcgcggg
     6121 gcttgccgat gcactaaccg caccgctcga ccataaagac aaaagtttgc agtctttgac
     6181 gctggatcag tccgtcagga aaaacgagaa actgaagctg gcggcacaag gtgcggaaaa
     6241 aacttatgga aacggcgaca gcctcaatac gggcaaattg aagaacgaca aggtcagccg
     6301 cttcgacttt atccgtcaaa tcgaagtgga cgggcagctc attaccttgg agagcggaga
     6361 gttccaaata tacaaacagg accactccgc cgtcgttgcc ctacagattg aaaaaatcaa
     6421 caaccccgac aaaatcgaca gcctgataaa ccaacgctcc ttccttgtca gcggtttggg
     6481 cggagaacat accgccttca accaactgcc tgacggcaaa gccgagtatc acggcaaagc
     6541 attcagctcc gacgatgctg gcggaaaact gacctatacc atagatttcg ccgccaaaca
     6601 gggacacggc aaaatcgaac acctgaaaac acccgagcaa aatgtcgagc ttgccgccgc
     6661 cgaactcaaa gcagatgaaa aatcacacgc cgtcattttg ggcgacacgc gctacggcag
     6721 cgaagaaaaa ggcacttacc acctcgccct tttcggcgac cgcgcccaag aaatcgccgg
     6781 ctcggcaacc gtgaagatag gggaaaaggt tcacgaaatc ggcatcgccg gcaaacagta
     6841 gcatttgaaa aatgccgtcc gaacacgata atttaccgtt cggacggcat ttttgcgcca
     6901 taccgcccta ggatacgacg gcacggcagg caagccggtt tcactatata atgccgtctg
     6961 aaccaacgag aaccacacca tgcaagccga tttcaaccgt cccgtcctgg ccatcgatac
     7021 cggaacttcc tatttgtcgc tcgcgctgcg cgccgacggc gaaacccgcc tgttccatca
     7081 agaggccggc agccgccaat ccgaactgat tctgccggaa atccgcactc tattccgaaa
     7141 tgcgggcatt accgccgccg atttgggggc gattgtttat gcgaaaggcc ccggcgcgtt
     7201 tacgggactg cgtatcggca tcggcgtggc gcaaggtttg gcaacgccgt ttgatacgcc
     7261 tttaatcggc atccccacgc tcgatgccgc cgcctcgctg ccgccgccgc aaagctgcat
     7321 actcgccgct gcggacgcgc gtatgggcga agtgttttat gcgtggttcg acacgctgaa
     7381 ccgccgccgt ttgagcggtt atcaagtcgg tagggcggca gacatccggc tgccggaggg
     7441 atgtgccttt tcagacggca tcggcaatgc gttcgcgctg gaaaaccgtc cgcccttctc
     7501 aggcaaaccc gatatgccga ccgccgccga cttcctcaat ctggcggctg aaggcggttt
     7561 tcccgccgtc gctgcggcac acgccgagct gctctacgtc cgcaacaaaa tcgccttgac
     7621 tgccaaagaa caggcggaac ggagggcgcg cctgtgaaca tccgccgcgc cgtttatgcc
     7681 gattgcgccg cactcgccgc gctcgatgcc gtctgcaacc cgtccgcgtg gacgcaacgc
     7741 caatttgagt ctgcattggt ttcgctgtcc gaacaggttt tccttgcgga aaaagacggc
     7801 gggattgccg cctttatcgt ttggcagaac ctgcccgacg aatccgaact gcacctgatt
     7861 gccaccgcgc ccgaatgccg ccgccgtggc gttgcgtccg ccctgctcga atattggttc
     7921 acacatctgc ccgaagacac gcaacgcctg ctgctcgaag tccgtgcagg caacaccgcc
     7981 gcacaggcac tgtacgcggc gcacggcttc agcattgcgg gcaggcggaa aaactattac
     8041 cgtacagccg acggtaaaac cgaagatgct gtcttgatgg agaaaatatg ttaagcgcgc
     8101 gctacctcca cctgcacgaa gccttgggtt tgggcccgat gtggttgaag cggggagccg
     8161 ttgtcctgcc gtcagcaaca ttaccggaaa gcccgacaca aatccgcccg caaaagcaaa
     8221 ccgtcctcag cattccgcag cgtccgtccg aacagcatac cggtcaggca cggctcgaaa
     8281 cgatgaaagc gttggaaacc gccgccgtac ctacgcgcaa acccgcgcct gaaaccgaaa
     8341 cgcctctgtc cggcgtttca gacggcatcg cccccgtttc cgccgcttcg ggcatcacca
     8401 agcttgccgt cgtcagtttg tgtccgccaa ccgaagatat ggtttacggg caactgttcc
     8461 acggcaaagc gggtgtcctg cttgacaata tactcaaagc cgtaggactg gatgccgcct
     8521 atgtccacaa aacctgttgg gtgaaaaccg ccgccgtcgg caacccgatg ccgtctgaac
     8581 aggccatcgc gaatgcgctg ggtcaaatcg cccgcgaact cgacagttgc cgcgcccccg
     8641 ccgtcctctt cctcggacag gcgtttgtca acccggaacg gcaggcgatg attgaaactt
     8701 tgtgcggcag ccgtcccttc ttcatcatcg accatcccgc ccggctgttg cgccaacccg
     8761 aacttaaagc ccgcgcctgg caggtgttga aacagttgaa acgcgccttg cggcaaggcg
     8821 gcggcagttg aagcgcaccg cacggggcgg tagaatcgca actgcgtccc aatatctgac
     8881 agaaagcaca aaatgaccga tttccgccaa gatttcctca aattctccct cgcccaaaat
     8941 gttttgaaat tcggcgaatt caccaccaag gcaggacgtc agtcgcccta tttcttcaat
     9001 gccggcctct ttaacgacgg cttgtccacg ctgcaactgg caaaatttta cgcacaatcc
     9061 atcattgaaa gcggcatccg attcgatatg ctgttcggcc ccgcctacaa aggcattatt
     9121 ttggcggcgg caaccgcgat gatgctggcg gaaaaaggcg tgaacgtccc gtttgcctac
     9181 aaccgcaaag aagccaaaga ccacggcgaa ggcggcgtgt tggtcggtgc gccgcttaaa
     9241 ggccgcgtgc tgattatcga cgacgtgatt tccgccggca catccgtacg cgaatcgatc
     9301 aagctgattg aagcggaggg cgcaaccccc gccggtgtcg ccatcgcgct cgatcgcatg
     9361 gaaaaaggca cgggtgagtt gagcgcggtt caggaagtgg aaaaacaata cggtctgccc
     9421 gtcgccccca tcgccagcct gaacgatttg tttattctgt tgcaaaacaa ccccgaattc
     9481 ggacagttcc tcgaacccgt ccgagcctac cgtcggcagt acggcgtaga ataaaaacaa
     9541 agcatatgcc gtccgaaccg ccttacgcct cagacggcat caaacctgac acacacgagg
     9601 aaataccatg cccgcctgtt tctgccccca ctgcaaaacc cgcctttggg tcaaagaaaa
     9661 ccagctcaac gtcgcccaag gcttcgtcgt ctgccaaaaa tgcgaagggc tgtttaaagc
     9721 caaagaccat ctggcaagca cgaaagaacc tatattcaac gatttgcccg aggctgtttc
     9781 ggatgtcaaa ctcgttcacc gtatcggcac gcgcgccatc ggcaagaaac agatttcccg
     9841 cgacgaaatc gccggcatcc taaacggcgg cacaaccctg cccgatattc cgcccgcaac
     9901 cgccgccacc cctgctgccg caccgcaggt taccgtaccg cccgccgcgc ccgcccgtca
     9961 ggatgggttc aactggacga ttgcaaccct gtttgccctt atcgtcctca ttatgcagct
    10021 ttcctacctc gtcatcctat gagcgcgccc gacctctttg tcgcccactt ccgcgaagcc
    10081 gccccctaca tccgccaaat gcgcggcaaa acgctggtcg ccggcataga cgaccgcctg
    10141 ctcgaaggcg ataccttaaa caagctcgcc gccgacatcg ggcttttgtc gcaactgggc
    10201 atcaggctcg tcctcatcca cggcgcgcgc cacttcctcg accgccacgc cgccgcgcaa
    10261 ggccgcacgc cgcattattg ccggggcttg cgcgttaccg acgaaacctc gctcgaacag
    10321 gcgcagcagt ttgccggcac cgtccgcagc cgttttgaag ccgcattgtg cggcagcgtt
    10381 tccgggttcg cgcgcgcgcc ttccgtcctg ctcatatcgg gcaacttcct gaccgcccgt
    10441 ccgataggtg tgattgacgg aaccgatatg gaatacgcgg gcgttatccg caaaaccgac
    10501 accgccgccc tccgtttcca actcgacgcg ggcaatatcg tctggctgcc gccgctcgga
    10561 cattcctacg gcggcaagac cttacatctc gatatgcttc aaaccgccgc ctccgccgct
    10621 gtctcgcttc aggccgaaaa actcgtttac ctgacccttt cagacggcat tgcccgcccc
    10681 gacggcacgc tcgccgaaac cctctcggcg caggaagcgc aatcgctggc ggaacacgcc
    10741 ggcggcgaaa cgcgacggct gatttcgtcc gccgttgccg cgctcgaagg cggcgtgcat
    10801 cgcgtccaaa tcctcaacgg agccgccgac ggcagcctgc tgcaagaact cttcacccgc
    10861 aacggcatcg gcacgtccat tgccaaagaa gccttcgtct ccatccggca ggcgcacagc
    10921 ggcgacatcc cgcacattgc cgccctcatc cgcccgctgg aagaacaggg catcctgctg
    10981 caccgcagcc gcgaatacct cgaaaaccac atttccgaat tttccatcct cgaacacgac
    11041 ggcaacctgt acggttgcgc cgccctgaaa acctttgccg aagccgattg cggcgaaatc
    11101 gcctgccttg ccgtctcgcc gcaggcacag gacggcggct acggcgaacg cctgcttgcc
    11161 cacattatcg ataaggcgcg cggcataggc ataagcaggc tgttcgcact gtccacaaat
    11221 accggcgaat ggtttgccga acgcggcttt cagacggcat cggaagacga gttgcccgaa
    11281 acgcggcgca aagactaccg cagcaacgga cgaaactcgc atattctggt acgtcgcctg
    11341 caccgctgac cgcaacggaa agccgccgca gaaatgccgt ctgaaccccg tttcagacgg
    11401 catttccccg attatatagt ggattaacaa aaaccagtac ggcgttgcct cgccttgtcc
    11461 tgatttttgt taatccacta taaagacctg cccaaccctc aaggaccccg atgaaatcct
    11521 accccgaccc ctaccgccat tttgaaaacc tcgattccgc cgaaacgcaa aacttcgctg
    11581 ctaaagcgaa tgccgaaacg cgcacgcgtt ttttagaaaa cgacaaggcg cgcgcgctgt
    11641 cggacggcat tttggcgcag atgcaggaca cgcggcagat tccgttttgt caggaacacc
    11701 gcgcgcggat gtaccatttc catcaggacg cggaatatcc gaagggcgtg taccgcgtgt
    11761 gtacggcggc gacttaccgt tccggctatc ccgagtggaa aatcctgttt tcagtggcgg
    11821 attttgacga gttgctcggc gacgatgtgt atttgggcgg cgtgtcgcac ttggtggaac
    11881 agcccaaccg cgcgctgttg actttgagca aatcaggcgg cgatacggcg tacacgctgg
    11941 aagtggattt ggaagcaggg gaattggtag aaggcggttt tcactttccg gcaggcaaaa
    12001 accatgtgtc gtggcgcgat gaaaacagcg tgtgggtgtg tccggcttgg gacgaacgcc
    12061 agttgaccga atcgggctat ccgcgtgagg tgtggctggt ggagcgcggc aaaagtttcg
    12121 aggaaagcct gccggtgtac caaattgccg aagacgggat gatggtgaac gcgtggcgtt
    12181 acctcgatcc gcaaggttcg ccgattgatt tgattgaagc gtcggacggt ttttacacta
    12241 aaacctattt gcaggtgtcg gccgaaggcg aggcgaaacc gttgaacctg cccgccgatt
    12301 gcgacgtggt cggctatctg gcggggcatc ttttgctgac gctgcgcaaa gactggaacc
    12361 gcgcgaacca aagctatccg agcggcgcat tggtggcagt aaaattgaac cgcggcgaac
    12421 tcggggcggc gcagcttttg tttgcgcctg atgaaacgca ggcattggaa agcgtggaaa
    12481 cgaccaagcg ttttgtggtg gcgagcctgc tggaaaacgt acaaggccgt ctgaaagcgt
    12541 ggcgttttac tgatggcaaa tggcaggaag ctgaactacc gcgcctgcct tcgggtgcgt
    12601 tggaaatgac cgaccagcca tggggcggcg atgtggttta ccttgccgcc agcgatttca
    12661 ccacgccgct gacgctgttt gcgctggatt tgaacgtgat ggaactgacc gtcatgcgcc
    12721 gccagccgca gcagtttgtt tcagacggta tcaacgtgca acagttttgg acggcttcgt
    12781 ccgacggcga acgcattccc tatttccacg tcggcaaaaa cgccgcgccc gacatgccga
    12841 cgctggtcta tgcctacggc ggtttcggca ttcccgaatt gccgcattat ctgggcagca
    12901 ttggcaaata ttggctggaa gagggcaatg cctttgtatt ggcgaacatc cgcggcggcg
    12961 gcgagttcgg cccgcgctgg catcaggcgg cgcagggaat cagcaaacat aaaagcgttg
    13021 atgatttatt ggcagtcgtg cgcgatttgt ccgaacgcgg tatcagttcg cccgaacaca
    13081 tcggcttgca gggcggcagc aacggcggac tgattactgc cgccgccttc gtgcgcgaac
    13141 cgcaaagcat cggcgcgctg gtgtgcgaag tgccgctgac cgacatgatc cgttatccgc
    13201 tgctctccgc cggttcaagc tggacggacg aatacggcaa tccgcagaaa tacgaagtct
    13261 gcaaacgccg gctgggcgaa ttgtcgccgt atcacaatct ttcagacgac gtgccatacc
    13321 cgcccgcgct tattaccacc agcctcagcg acgaccgcgt ccatcccgcc cacgcgctca
    13381 agttctacgc caaactgcgc gaaacctcgc cgcaatcttg gctctactcg cctgacggcg
    13441 gcggccatac cggcaacggc acgcagcgcg aagccgccga cgaactcgcc tgcgtgttgc
    13501 tgtttttgaa agagtttttg ggctaagggc ggggagcggc actgccgccg cgaatgaaaa
    13561 aaggtcgtct gaaactgctt tttcagacga ccttggattc ggatttcaag tgcaacacta
    13621 gtgtattagt ggttggaaca gattcaagaa taaaacactt ggcgtttcgt agccaagtgt
    13681 ttttcttggt cggtggttca actcatcttg aaccctgcgt atctcccgat tgctgatgtt
    13741 tcggaaatcg gtttgtttgg ggaagtattg ccggatgagt ccgttggtgt tctcattcag
    13801 ccctttctcc caagaatggt aagggcgac
//
LOCUS       DAWFIE010000056        13666 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWFIE010000056 DAWFIE010000000
VERSION     DAWFIE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13666
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.056"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(259..810)
                     /locus_tag="WGQ71_001464"
     CDS             complement(259..810)
                     /locus_tag="WGQ71_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0707456.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKVKQRNEELEAAEEAAAQEALGREQEAARVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGKTPNGIKFSELACKTAKTEARLEELH
                     NRKKALIDEMAREADKKELSKRL"
     gene            complement(824..2470)
                     /gene="pgi"
                     /locus_tag="WGQ71_001465"
     CDS             complement(824..2470)
                     /gene="pgi"
                     /locus_tag="WGQ71_001465"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0707457.1"
                     /translation="MKHFHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            complement(2591..3439)
                     /gene="hexR"
                     /locus_tag="WGQ71_001466"
     CDS             complement(2591..3439)
                     /gene="hexR"
                     /locus_tag="WGQ71_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ0707458.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            complement(3489..4475)
                     /locus_tag="WGQ71_001467"
     CDS             complement(3489..4475)
                     /locus_tag="WGQ71_001467"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ0707459.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            complement(4456..5151)
                     /gene="pgl"
                     /locus_tag="WGQ71_001468"
     CDS             complement(4456..5151)
                     /gene="pgl"
                     /locus_tag="WGQ71_001468"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ0707460.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            complement(5431..6876)
                     /gene="zwf"
                     /locus_tag="WGQ71_001469"
     CDS             complement(5431..6876)
                     /gene="zwf"
                     /locus_tag="WGQ71_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ0707461.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMMQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAARGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            7394..9229
                     /gene="edd"
                     /locus_tag="WGQ71_001470"
     CDS             7394..9229
                     /gene="edd"
                     /locus_tag="WGQ71_001470"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ0707462.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAIGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALIGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            9426..10064
                     /locus_tag="WGQ71_001471"
     CDS             9426..10064
                     /locus_tag="WGQ71_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ0707463.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSYALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPEYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            10079..10375
                     /locus_tag="WGQ71_001472"
     CDS             10079..10375
                     /locus_tag="WGQ71_001472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707464.1"
                     /translation="MPSEKPFPFQTAFCRLRAQSAYTAALIRHTAPKMPIRLPHNKNN
                     VQFIRQQPDSPYYAAEKMPSETHSDGIHLKKTTDSTPINPLPKPLSSLGFRRRV"
     gene            complement(10323..11364)
                     /locus_tag="WGQ71_001473"
                     /pseudo
     CDS             complement(10323..11364)
                     /locus_tag="WGQ71_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            11527..12567
                     /gene="mutY"
                     /locus_tag="WGQ71_001474"
     CDS             11527..12567
                     /gene="mutY"
                     /locus_tag="WGQ71_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ0707465.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVGQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIIRNRNGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPTHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            12984..13544
                     /locus_tag="WGQ71_001475"
     CDS             12984..13544
                     /locus_tag="WGQ71_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ0707466.1"
                     /translation="MNMKTLLALAVSAVCSVSVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT         3199 a         3689 c         3452 g         3326 t
ORIGIN      
        1 gtatcggttt aaatttaatt cactataaaa acgacaaaaa cgcaaaagcc gccgacattc
       61 ccgcatccaa gtttcagtca atcagataac cttggatttc tttggttttc gcattgattt
      121 ctctggtacg gcagtcagat tgtgccacgc cgtattcgtc gccgtcggcg catttggcat
      181 tcaaaccgtt ttgtcagttg cggtactcgg gcatgacggt ttcggcaata tcggcgggca
      241 gtgcctgaac cgcgctgttc agagccgctt tgacagttct ttcttgtccg cttccctggc
      301 catttcgtcg ataagggctt ttttacggtt gtgcagctct tccaagcgtg cttcggtttt
      361 cgccgttttg catgccagtt cgctgaattt tatgccgttt ggcgttttac cttcagcttt
      421 cgcattgttg gcacagattt tatccatccc gcttttccat gttttctgtg aggcttgcag
      481 cttattctgt acggtttgag gcaatccttt ccagaactgc tcgaactcgc tgcgcgacag
      541 cttgtagcgt tcttcccatt cggatacgcg ggcggcttcc tgctcccgac ccaatgcctc
      601 ctgcgccgcc gcttcttctg ccgcttcaag ttcttcgttc ctttgtttca ctttgtccaa
      661 cggctcttta atcagtgcca tagctgcgga acatatatgt ttaaatttat gcaaaccatc
      721 atatcgggat tgcacacgct ctgcaagttt accgacggtt ttcctgttcg ataaaaatgc
      781 cgtttgaaac ggtcggcgtt cagacggcat ttttccgcag gttttatttg cggttggtct
      841 gcaggtagag gttgatcagg cgttcggtcg aactgtcgtg cttttgcggc ccggtttcgc
      901 cggtcagttc gcccaaaatg gttttagcca gttgtttgcc gagttccacg ccccactggt
      961 cgaagctgtt gatgccccaa atgatgcctt gtacgaaggt tttgtgttcg tacatggcaa
     1021 tcaggctgcc catattgcgc gggttgacct tgtccatgag aatgaggttg gtcgggcggt
     1081 tgccggagaa ggttttgtgc gggaccagct cttcgatgcg cgcctcatcc ataccctgcg
     1141 ctttgagttc ggcacggact tcgtcggggg ttttgccgcg cataaaggct tctgcttgtg
     1201 caaagacgtt ggcaagcagg atttcgtggt gtccgggcag gttgctgcgt ttttcaagtg
     1261 aggcaatcag gtcgatgggg gtaatgtgcg tgccttggtg cagcagttgg aagaaggcgt
     1321 gctggccgtt aatgcccgtt tcgccccaga taatcggcga ggtttcgtgt ccgactgctt
     1381 tgccgtccaa cgtaacctgt ttgccgttgc tttccatatc gagctgctgg atgaatttgg
     1441 gcaggcggtg caaatgttgg tcgtaaggcg cgatgacgtg gctgccgccg ccgtagtagt
     1501 tgatatacca gatgccgatg agggcgagaa tgacgggcag gttgcgctcg agcggtgtgt
     1561 tgatgaagtg ttggtccatc aggtgcgcgc cgttgagcat ttcgatgaag ttttcttcgc
     1621 cgagatacag cataatcggc aatccgatgg cggaccacag gctgtaccga ccgccgaccc
     1681 aatcccaaaa ttcaaacata ttggcggtgt cgatgccgaa ttcggcgacg gctttttgat
     1741 tggtggaaac ggcggcgaag tgtttggcaa cggcttcttc gtcgcccgca tgattcaaaa
     1801 accattcgcg cgcggtcagc gcgttggtca gcgtttcctg cgtggtaaat gttttggagg
     1861 cgatgatgaa caacgtggtt tcggggtgga ctttggacaa tacgtcgcgc agttgcgagc
     1921 cgtccacgtt ggagacgaag tgcatattga ggcgcggatg accgaaaggt ttgagcgcgg
     1981 tacacatcat cagcggaccc aaatccgatc cgccgatgcc gatgttgaca acgtcggtaa
     2041 tgacttggtt ggtatagccc agccagcttc cgctgcggac ttcgtgtgca aattcgccca
     2101 tacgttgcaa aacgcggttg actttgggca tcacatcttc accgtcaacc acaatcggcg
     2161 aattggtgcg gttgcgaagg gcgacatgca ggacggcgcg gttttcggtg gtattgattt
     2221 tttcgccgtg gaacatctgc cgcatccgct ccggcacgcc tgcttctcgg gcaagctcga
     2281 acaaaagcga catggtttcg tcgttgatgc ggtttttgga gtagtccagc gtcagtccgc
     2341 cgacttgcag ccagtagcgt tccgcacgct gcgggtcttg ctcgaacatt tcgcgcatat
     2401 gcaatgtttt gctgtcgtca aagtggttcc acaatttcga ccatgcgggt aagtcgtgaa
     2461 agtgtttcat ctatatgctc ctgaatgagg ttttttgttg tgggatgaaa aggctgctgg
     2521 aaactgccgc aagccgccga cgaccgttgt tcggcatttc agacggcatt tgtgggatgc
     2581 cgtctgaagg tcaatctttg tcgtaatcga tgtgcttgtt gtgtatgctt tttttgcttt
     2641 tctgcaattg caggctggca gcatcgccca agcgcagggc aagtccgatg gcgagaatgt
     2701 cgatgacggc aagctgcaag aggcgggaaa ccatgggcgt gtagagttcg gcattttcct
     2761 gtgtggcaac gctcaacacg cagtcggcaa gttgcgccag aggcgaatcg ttgcgggtca
     2821 gtgcgatgac agacgcgccg ttttctttgg cgatgctgac cgcatccaaa agttcgatag
     2881 acgaacccgt gttggaaatg gcaaccaaaa catcctgatc gctcaaaaca gatgccgcca
     2941 tcagctgcgt gtgcgtatcg acataggcga cggtggacat gccgaaacgg aaaaatttat
     3001 gctgcgcgtc ctgtgccaca atgccggaat tgccgacacc gtaaaactcg acgcgacggg
     3061 cgtgcatcag cgtggcaatg gcgttttcca gctccgactc tttcaggaag cggcgttcgc
     3121 ccaacagcga ggcggcggca ttgcccaaca ctttctcgac cacgcttgcc atatcgtcgt
     3181 cggcgttgag ttcttcgtgg acatagggca taccctcatg accgatgctg gcggacaagg
     3241 cgagcttgaa ctcgggcagc cctttataac ccaagctgcg gcagaatcgg atgacggtcg
     3301 gctggctgac ggacgcacgt tcggcaattt cggcaacggc ggcatggacg aaccatttgg
     3361 gttccgccaa tgcacattcg gcgactttgc gttccgcacc ggaaaggttt gccagtgatt
     3421 cgctgatttt gcttaacata atgatatgcc cttcgataat gcagccccgc tgcaaggagc
     3481 cgctgtggtt aaacgtttct caaatggttg tcgagagccg cagccgcacc ggaaattccg
     3541 ggaaactcgc tcaagacgac atacacggga atcgcggcaa gatatgcttc aaacctgccc
     3601 ttgttctcga aacggctgcg gaacggggaa gttttgaaat attccaacac gcggggaata
     3661 atgccgccgc acaggtacac gccgccgcgc gcgcccagcg tcagggcgag gttggaagca
     3721 accgtgccga gcatggcgca gaagatgtcc aaagtctgac ggcacaaagg cgacgcgccg
     3781 ctcaaagcct tttccgtgat ttcagacggc atcagtttgg cgggtttggc tttctgtttt
     3841 gcagccaaag cctcgtaaac caagctcaag cccgcgccgc tcaaaaagcg ttcggcggaa
     3901 acatggccgt atttgttttt ggcgtactgc caaatcagca cttccatatc gtcaaacggc
     3961 gggaaactgg tatgcccgcc ctcgcccgcc aaagccaccc agcctgcgtg gctgtgcacc
     4021 aatccgctca cgcccaggcc ggtaccgggg ccgataacgg ctttgggggc aaattcgaca
     4081 ggcttttgcc cgcctacctg catcaggtct ttgcttgaag tctgcgttac cgccaatgcc
     4141 tgcgcggtaa agtcgttcaa aaggatgagg gtgtccagcc ccaaagtctg acgggtggtt
     4201 tcgatggaaa acgcccaatg gtggttggtc atctgcaccc agtcgcccaa aatcgggttg
     4261 gcgatggcaa atgccgcgtg ccgtacggct gttgcaccgc tttgattcag ataggcacgc
     4321 accgcatcgg taaccgtatc gtagtcttta cacggaagca cggcggcttt ttcaatgacg
     4381 cgcggcgcgg tttccagcgc aaagcgtgca ttcgtcccgc cgatatcggc gaccagtcgg
     4441 ggatatccgg cttgtttatt cggcgtagaa gacatggcag ttcactcctt gatggttcaa
     4501 aacgaggttg atcggatatt cgcggttttc gccttgtgcg gcttggtcga acacggcttt
     4561 tttctcttcg ccccgtatcg ccaaaaacac atgccccgta tgggcaatcg catccaaggt
     4621 catactgacg cgctcgtgcg gcgcggtaac gggcgtggta tgcaccagcg cgacacctgc
     4681 cgaaccgtcg attgccgtct gaaactgcgg agctttcggg aaaatcgaag ccgtatgccc
     4741 gtcgtttccc atacccaaaa ccaaaacatc gggctgtttg taatgtttca gtgcataatc
     4801 gacaacagca tcgggatgta attcggtttc agtttttccg tcttccacca taggaatcca
     4861 cattgccgct tccgctttgt tcttcaacag gtattcgcgc accaaaccgg tattgctgtc
     4921 ggcgtggacg gtcggcacga tgcgttcatc tgccaaggtg atgccgacgt ttttccaatc
     4981 caaatctttt tgcgacaggg cgttgaaaaa tgcaatcggc gaacgtccgc cggaaactgc
     5041 caacaccgca ccgcccttct cgtccagtgc gccctgcaaa gcatccgcca ctgcgtcagc
     5101 caaagactgc gccgcttctg ccgcattttc gtattcgtgc caaacaaaca tatttgtgtc
     5161 ctttttttat ttcagacggc atattccgtt atggaaacgg gttgagcaat atgtcggccg
     5221 aacagttgtt tatgcttttg ataccaaata tcgggactgc tttttatagt ggattaaatt
     5281 taaaccagta cagcgttgcc tcgccttgcc gtactatttg tactgtctgc ggcttcgtcg
     5341 ccttgtcctg atttttgtta atccactata ctgctttact tacgttcaga cggcatttca
     5401 aaccccatgc cgtctgaacg cattattgta ttactgctct tcgtgccact tgtgtccgtc
     5461 gcgcgccaat agttcgcgcg cggcttcagg cccccacgag tgtgcgccgt agccgtgcgg
     5521 cggcgtggtg ttatttgtcc agttttccaa aatcggcatc acatattccc acgcggcttc
     5581 gagttcgtcg cggcggttaa acaaagcgag tttgccgtta atcacatcca gcagcaggcg
     5641 ctcgtaagct tccgcgcggc ggctttccaa tgctttgccc aaatcggttg ccagcggcac
     5701 ggtttcgacc ttatttcctg cccccggggt tttcatctgc gtatagaggc gcacggattc
     5761 atatggttgc aactcgataa cgagccggtt gggcgcggtg cggctgcctt caaaaatatg
     5821 gctgttcaaa tctttgaagt tcaaaacgat ttccgccact ttgcccgcca tgcgtttgcc
     5881 ggtacgcagg tagaagggaa cgcccttcca gcgttcgttt tcgatttcgg ctttaatggc
     5941 gacgtaggtt tcggtaaagc tgtcttgcgg aacgttgatt tcttcaagat agccgttcat
     6001 gcctctggcg gcggtatatt gtccgcgcac gacgttttca ttgacagact cgacggtcag
     6061 cggcttcaat gacttgatga ctttgacttt ttcatcgcgc accgcgtcgg catccaagct
     6121 ggcgggggct tccatcgcag tcatgcacag catctgcatc aaatggtttt gcatcatatc
     6181 gcgcaacgcg ccggtaatgt cgtaaaactc gccgcgctct tccacgccca actgctcggc
     6241 gatggtcaac tgcacgcttt cgatgtattt attgttccac agcggctcga acattacatt
     6301 ggcaaaacgc agcgcaagca ggttttgcag gctttctttg ccaaggtagt ggtcgatgcg
     6361 gtaaatttgc ccttctttga aataacgcgc aacatcggta ttgatttgct gggaagaagc
     6421 caaatccgta cccaacggtt tttccaaaac cacacgcacg ttgtcggcgt tcaaaccgat
     6481 tttggcaagg ttttcacatg tttgcgcgaa gaatttcggc gcggtggaaa gatagatgac
     6541 gacattgtcg gtttctttgc gcgctttgac caaatcgccc aaagcggcaa aatcgtccgg
     6601 ctgcgtaaca tcgactttga gatatgcgag acgttcgaca aacgatgccc aagcctcatc
     6661 ggaaaaattt tctttcacat ggattttgga actggtttcc accttcgcca gaaaaccttc
     6721 ggtatccaac tcgctgcggc tgacccctaa aatacgccct tcgggatgaa gcagaccggc
     6781 aacatgcgcc tggtacagac agggcaacag cttgcgcatc gccaaatcgc cggtcgcacc
     6841 gaacaacacc aaatcaaaat ttgtttgtgt actcatcgta ttatctcgtc aggaaagaat
     6901 ttttcgatgc cgtctgaaac ctgtttcccc catcacgctg catcgcaata tcggaaacaa
     6961 aggcaggcgg cataatgagt agtaatacta cacaccgcta cactttttgt ctattcccat
     7021 ttttacaatt tatttgacct agtccaaaaa tcgggcaggt ttcccctatt ccgttacaac
     7081 aatcggaaga ttctgcgatt taaatcaaat ttcttttcga tgtctgattt ttttgtaaca
     7141 aaattacaaa ttttgtacta taataacacc cgcttcccac tttcagacgg tatacctttt
     7201 aaaatatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac agtacagata
     7261 atacggcaag gcgaggcaac gccgtactgg tttttgttaa tccactatac ttaccgtctg
     7321 aatacccgat acaaaaatca gaaacgcaca aacaaatccc caataccccc cccgttccga
     7381 caggagaccg accgtgaaca ctactcctat ccactccaaa ctcgccgaaa tcaccgggcg
     7441 cattattgaa cgcagccgtc cgacgcgtga aaaatatctg gcgaagatcc gcagtgccaa
     7501 acaaatggga cgcttagagc gcaaccagct cggctgcagc aacttggcac acggctatgc
     7561 tgccatgcct aaaagtatca aaatcgaaat gcttcaggaa accgtcccca acttaggcat
     7621 catcaccgcc tacaacgaca tggtttccgc acaccagccg tttaaagact tccctgacca
     7681 aatcaaagac gaagcgcaga aaaacggcgc gaccgcccaa gtcgccggcg gcacgcccgc
     7741 catgtgcgac ggcatcacgc aaggctacgc cggcatggaa ttgtcgctgt tctcccgcga
     7801 cgtgattgcc atgagtaccg ccatcgggct gtcgcatcaa atgtttgacg gcagcctgtt
     7861 tatgggcgta tgcgacaaaa tcgttccagg tttgatgata ggcgcgcttt cgttcggtca
     7921 tattccgggt atcttcgtcc ccgcaggccc gatgtccagc ggtatcggca acaaagaaaa
     7981 agcccgcacc cgccagcttt tcgccgaagg caaggtcgga cgcaacgaac ttttgaaaag
     8041 cgaaatgggt tcttaccaca gcccgggcac ctgcactttc tacggcacgg caaactccaa
     8101 ccaaatgatg atggaaatga tgggcgtgca cctgcctgcc gccgccttcg tccaccctta
     8161 caccgacctg cgcgaagcgc tgacccgcta cgccgccgga cacctcgcgc gcggcatcaa
     8221 aaacggcacg attaaacctt tgggcgaaat gttgaccgaa aaatccttta tcaacgcctt
     8281 gattggcctg atggcaaccg gcggttcgac caaccacacc atgcacctcg tcgctatggc
     8341 gcgtgcggcc ggcgtgattt tgaactggga cgacttcgac gaaatttcct ccatcatccc
     8401 gctgctcatc cgcgtttatc cgaacggcaa ggccgacgtg aatcacttta ccgcagcggg
     8461 cgggctgcct ttcgttatcc gcgaattgct gaatgcaggc ctgttgcacg acgatgtcga
     8521 taccgtcgtc ggacacggta tgcgccacta caccaaagag cctttcctca tcgacggcaa
     8581 actcgaatgg cgcgaagccc ccgaaaccag cggcaacgac gacatcctgc gcaaagctga
     8641 caacccgttc tcccccgacg gcggtctgcg cctgatgaaa ggcaacatcg gacgcggcgt
     8701 gattaaagtg tccgccgtgc gcgaaggttg ccgcatcatt gaagcgcctg ccatcgtgtt
     8761 caacgaccaa cgcgaagtgt tggctgcgtt tgaacgcggc gagttggaac gcgattttgt
     8821 gtgcgtcgtc cgctaccaag gcccgcgtgc caacggtatg cccgaattgc acaaactgac
     8881 cccgcctttg ggcatcctgc aagaccgcgg cttcaaagtg gcgctgctga ccgacggccg
     8941 tatgtccggc gcgtccggca aagttccggc ctccatccac atgacgcctg aagccttgat
     9001 tggtggcaac atcgccaaaa tccgtaccgg cgacctgatc cgcttcgact ccgttagcgg
     9061 cgaactcaac gtcctgatta acgaaaccga atggaatgcc cgcgaagtcg aaagcatcga
     9121 cttgggcgcg aaccaacaag gctgcggccg cgaactcttc gccaacttcc gcagcatgac
     9181 cagcagcgca gaaaccggtg ccatgagttt cggcggcgaa tttgcctgat gcgcgtttca
     9241 gacggccttt tcagaccgaa ggccgtctga aaaattattg aagctgttta aacggatcag
     9301 acgcagcgtg ggctttgccc gcgaataagc aacttccccc catttttcaa aaaggccgtc
     9361 tgaaaaccta gccccttcct gtttgacctc aacaaaaatg cccctatccc acttggagaa
     9421 ctgaaatgtc caaactgacc ccacgcgaaa tcctgaccgc cggcgcagtt gtgcctgtga
     9481 tggcgattga cgacttaagc accgccgtcg atttgtccta cgcccttgtc gaaggcggca
     9541 tccctaccct cgaaattacc ctgcgtacac ccgtcggcct cgacgccatc cgcctgattg
     9601 ccaaagaagt gcccaacgcc atcgtcggcg cgggtacggt aaccaatccc gaacagctca
     9661 aagccgtcga agacgcaggc gcggttttcg ccatcagccc ggggctgcat gaatccctcg
     9721 ccaaagccgg ccacaacagc ggcatccccc tgattcccgg tgttgccacc ccgggcgaaa
     9781 tccaactggc tttggaacac ggcatcgaca ccctcaaact cttccccgcc gaagtcgtcg
     9841 gcggcaaagc catgctcaaa gccctgtacg gcccttacgc cgatgttcgc ttctgcccga
     9901 caggcggcat cagcctcgcc accgcgcccg agtacttggc actgcccaac gtcctgtgcg
     9961 tcggcggctc ttggctgaca ccgaaagaag ccgtgaaaaa caaagactgg gacaccatca
    10021 cccgcctcgc caaagaagcg gcggcgttga aacccaaagc ctgattcgca tcgtaaaaat
    10081 gccgtctgaa aaacctttcc cgtttcagac ggcattttgc cgattgaggg cacagtcggc
    10141 atacacggca gcactgatca gacataccgc ccctaaaatg cccatccgcc ttccgcataa
    10201 taaaaataac gttcagttca ttcgacagca gccggacagc ccatactacg cggctgaaaa
    10261 aatgccgtct gaaacgcatt cagacggcat ccacttaaaa aaaacaactg attcaacgcc
    10321 gattaatccg cttccaaaac cactttcatc acttggtttt cggcggcgtg tttgaacacg
    10381 tcgtaggctt tttccaattc actgaatttg aaatgatggg tcagcatttt ggtgtaatcg
    10441 acggagctgc tggaaatcgc cttcatcagc atttcggtgg tattggcgtt taccagaccg
    10501 gtagtaatgg cgaggttttt aatccagagt ttttccagtt tgaaatcaac ggattgacca
    10561 tgtacaccaa ccacagcgat atggccgccg ggtttcacaa tgtcttggca catattccat
    10621 gtagcaggga taccgacggc ttcgatggcg caatccacgc cgtcttcgcc gacgatggca
    10681 aagacttgtt tggatacttc gccggaagca gggttaatgg tatgggtcgc acccaattct
    10741 ttcgccagtt tcaaacggtt ttcgtccata tcgcaaacga tgatggcggc gggactgtac
    10801 agttgggcgg tcaacagggc ggacataccg acagggcctg ccccagcgat gaatacggtg
    10861 tcgccgggtt tgacatcgcc gtattgcacg ccgatttcgt gggcggtcgg caaagcgtcg
    10921 ctcaacaaca gggcgatttc ttcgttgaca ttatcgggca gcggaacgag gctgttgttg
    10981 gcataaggcg tacggacgta ttcggcctga gtaccgtcaa tcatgtaacc caaaatccaa
    11041 ccgccgttac ggcagtgtga atagagttgg gttttgcagt tgtcgcaagt gcaacatttg
    11101 ctgacgcatg aaataatgac tttatcgccg actttgatgt tttttacagc ctcgccgact
    11161 tcttctacaa taccgatgcc ctcatgaccg agaatacggc cgtcggcgac ttcggttttt
    11221 tttgcctttc caaataccca aatcggtacc gcaaatcgtg gttttgacga ttttcaccac
    11281 cgcatcggtc ggatcgataa tctgcggacg gggtttttct tcaaaacgga tgtcgtttgc
    11341 gccgtgataa accattgctt tcatgctgat actccttgct tgttgataaa taatttcaat
    11401 accgcaataa agtttcctta tatgagttat atgcccctac aaaaaataag tcaataagaa
    11461 ttattttcac aatgttatac aataacatac tattttaaat ataaataaaa ccaccgattg
    11521 atattaatga acacacccat ccccttctcc gaacggctca tccgctggca aaaacaacac
    11581 ggtcgccacc acctcccttg gcaggtcaaa aacccttatt gcgtctggct ttccgaaatc
    11641 atgctccagc aaacgcaggt cgccaccgtg ttggactact atccgcgttt cttagaaaaa
    11701 ttcccgactg ttcagacgct tgccgccgcg ccgcaagacg aagtgttgtc gttgtgggcg
    11761 ggcttgggct attacagccg cgcgcgcaat ctgcacaaag ccgcgcaaca agtcgtcgga
    11821 caattcggcg gcacatttcc atcggagcgt aaagacttgg aaaccctctg cggcgtaggc
    11881 agaagcaccg ccgccgccat ttgcgccttc tccttcaacc gccgcgaaac cattttggac
    11941 ggcaacgtca aacgcgtact ctgccgcgtg ttcgcccgcg acggcaatcc gcaggacaaa
    12001 aaatttgaaa actcgctctg gacacttgcc gaaagcctgc tgccgtctga aaacgccgat
    12061 atgcctgcct atacacaagg tttgatggat ttgggcgcga ccgtgtgcaa acggacgaaa
    12121 cccttgtgcc accaatgccc gatggcggac atctgcgaag cgaaaaagca aaaccgcacc
    12181 gccgagctgc cgcgcaaaaa aaccgccgcc gaagtaccga ccctgccgct ttactggctg
    12241 attatccgca accggaacgg cgcgattttg ctggaaaaac gccccgccaa aggcatttgg
    12301 ggcgggctgt attgcgtgcc gtgttttgaa agtttgaacg ggctttccga ctttgccgcc
    12361 aaattctccc tgaccatggc agatatggac gaacaaaccg ccctgaccca ccggctgacg
    12421 caccggttgc tgatgattac gccgtttgaa ggacaaatgc cgtctgaaag cccttcagac
    12481 ggcatttgga taaagccgac gcatttgaaa gattacggtt tgcccaagcc tttggaaatt
    12541 tatttaaacg gtaataggtt agaataaaca aaataaaccc attgaactgt tgtttgcagg
    12601 tatcgcagca agaacaaccg atgaatttgg gtcgtatttt aggcggcggg ataatgttca
    12661 aatgggacat ttggaacgga agaagtcggc aatttaaaaa ggatttaaaa agcaaagaag
    12721 gtcaaaaaca tgaacacaaa cttaaatgac aaagacaaag ccatggatac cgcaatcagg
    12781 tttcagaaaa ggatgaggat tccgaaattt ttctttttaa ttctcggaat cacaatggtt
    12841 ttggcattta tccaagacgt gataacgggt tctaattttc tgcaaataac aattaatgta
    12901 aaattttcgt aaaaatttat cggcttttaa aacaaaattg actaaaatag tcgcgagttt
    12961 ttactgcaat aaaggagatt gcaatgaata tgaaaacctt attagcacta gcggttagtg
    13021 cagtatgttc agttagtgtt gcgcaagcac acgagcataa tacgatacct aaaggtgctt
    13081 ctattgaagt gaaagtgcaa caacttgatc cagtaaacgg aaacaaagat gtgggtacag
    13141 tgactattac tgaatctaac tatggtcttg tgtttacccc tgatttacaa ggattaagcg
    13201 aaggcttaca tggtttccac atccatgaaa acccaagctg tgagccaaaa gaaaaagaag
    13261 gtaaattgac agctggttta ggcgcaggcg gtcactggga tcctaaaggt gcaaaacaac
    13321 atggttaccc atggcaagat gatgcacact taggtgattt acctgcatta actgtattgc
    13381 atgatggcac agcaacaaat cctgttttag caccacgtct taaacattta gatgatgttc
    13441 gcggtcactc tattatgatc cacacgggtg gtgataatca ctccgatcat ccagctccac
    13501 ttggcggtgg cggcccacgt atggcatgtg gcgtgattaa ataattcgat tgttcgaaac
    13561 gaaaagtgcg gtaaattttg accgcacttt tttgctagat atttagcatt gagacctttg
    13621 caataacata ggttactaaa attttatgct caatctcatt ttcaaa
//
LOCUS       DAWFIE010000057        13393 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWFIE010000057 DAWFIE010000000
VERSION     DAWFIE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13393
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.057"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..891)
                     /gene="mafA"
                     /locus_tag="WGQ71_001476"
     CDS             complement(<1..891)
                     /gene="mafA"
                     /locus_tag="WGQ71_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0707467.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVE"
     gene            complement(1075..1794)
                     /locus_tag="WGQ71_001477"
     CDS             complement(1075..1794)
                     /locus_tag="WGQ71_001477"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ0707468.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(2012..2866)
                     /locus_tag="WGQ71_001478"
     CDS             complement(2012..2866)
                     /locus_tag="WGQ71_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ0707469.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(2995..3723)
                     /gene="rpsB"
                     /locus_tag="WGQ71_001479"
     CDS             complement(2995..3723)
                     /gene="rpsB"
                     /locus_tag="WGQ71_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ0707470.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(3900..4133)
                     /locus_tag="WGQ71_001480"
     CDS             complement(3900..4133)
                     /locus_tag="WGQ71_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707471.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMKQQEQAEFEQQRAQRQKDSGTKP"
     gene            4211..4792
                     /locus_tag="WGQ71_001481"
     CDS             4211..4792
                     /locus_tag="WGQ71_001481"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ0707472.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINHLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRAAQKRGVKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            4853..5443
                     /locus_tag="WGQ71_001482"
     CDS             4853..5443
                     /locus_tag="WGQ71_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0707473.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            5981..7447
                     /locus_tag="WGQ71_001483"
     CDS             5981..7447
                     /locus_tag="WGQ71_001483"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ0707474.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(7635..8009)
                     /locus_tag="WGQ71_001484"
     CDS             complement(7635..8009)
                     /locus_tag="WGQ71_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0707475.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(8247..8546)
                     /locus_tag="WGQ71_001485"
     CDS             complement(8247..8546)
                     /locus_tag="WGQ71_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0707476.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            complement(8701..9480)
                     /gene="map"
                     /locus_tag="WGQ71_001486"
     CDS             complement(8701..9480)
                     /gene="map"
                     /locus_tag="WGQ71_001486"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ0707477.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            complement(9520..10170)
                     /locus_tag="WGQ71_001487"
     CDS             complement(9520..10170)
                     /locus_tag="WGQ71_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707478.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRGKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            complement(10178..10786)
                     /locus_tag="WGQ71_001488"
     CDS             complement(10178..10786)
                     /locus_tag="WGQ71_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ0707479.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(10850..11443)
                     /locus_tag="WGQ71_001489"
     CDS             complement(10850..11443)
                     /locus_tag="WGQ71_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ0707480.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFNHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPYPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            complement(11448..11795)
                     /locus_tag="WGQ71_001490"
     CDS             complement(11448..11795)
                     /locus_tag="WGQ71_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ0707481.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIQNITG"
     gene            11841..12716
                     /gene="rsmI"
                     /locus_tag="WGQ71_001491"
     CDS             11841..12716
                     /gene="rsmI"
                     /locus_tag="WGQ71_001491"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ0707482.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLAGRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMVELFSERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            complement(12751..13156)
                     /locus_tag="WGQ71_001492"
                     /pseudo
     CDS             complement(12751..13156)
                     /locus_tag="WGQ71_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(13275..13385)
                     /locus_tag="WGQ71_001493"
                     /pseudo
     CDS             complement(13275..13385)
                     /locus_tag="WGQ71_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
BASE COUNT         2801 a         3170 c         3893 g         3529 t
ORIGIN      
        1 ctctacggat ggggcggagt tacccgtatg attgccgtat ggtcggatat cggagaaatc
       61 gaccattaat ccttccgtcg gtttaattcc tttgcttact ttatacggcc ccatccacaa
      121 tgcgtaattt tctttatagg cagcttcaaa cgcattggtt ttgggtttga tgagcaattt
      181 tttattggtt ctgtctactg cgaaatattc cagttttgtt tgggctttca gtgtttcggc
      241 attgtatagg tgcatttcgg ttctgttgcg tatcgttccg aatacgtcga tgttaataaa
      301 cacatctgta tcggcattgg caggagaaac aacgtctatg ccgcgcagga aaaatacggt
      361 ctgtaccaag tgggaaagaa aggcagtgtc gcgcgggtta gtcgtcaagg tttcatttcg
      421 ataatccccc atcccgccaa tatttaagcc cagactgctt ttacttccgc taccgtctga
      481 ttgggtgcga gagagtgcag gggcattaag tgtagataaa gaagtggtta aacctgtcaa
      541 accgcctgat gttgtttcag cggtggtttc gtaacgtgga taggtgtaat cggtacggac
      601 ggcagggctg tttatgtatt cgccacgaat cagtgcatca atggagtagc gaccccctgt
      661 caaactgcct gaaccttggt cgcccatagt tgcaatgtac aatgcaactt ttcgtccgtg
      721 taatgcctgt aaatccatgt ctttaacggc agctctggca gaagcggcca caagttcttg
      781 ttcgaccgca aagcgtttac ctccgccatg cgatggaata cctgtcagtg tcccgcaggc
      841 ggataaaata aaaactgaaa aaagaatagg tatcagcagc cgtgcttgca tagattttct
      901 cctttgatga aaaacaaatt gtatcaaaat tgtaaatata gtggattaaa tttaaaccag
      961 tacggcaagg cgaggcaacg ccgtactggt ttaaatttaa tccactataa tctccatata
     1021 ataaatggct gtctgaagcc catatgcgac tatatctgcc gacactatgg tcaatcagca
     1081 gtgaaccagc gtaccttcgt tttcaccggt tactacacgt ttgagcgagc cttctttagc
     1141 aatgccgaat actacaatat tgagcttgcg ttcgcggcag agggcgaaag cggtggcatc
     1201 catcactttg aggtttttca acaaggcttc gtcaaaagta atggtttcgt agcgcgtggc
     1261 ggatgagtct tttttggggt ctgcggtgta cacgccgtcg acgttggtgg ctttaagcat
     1321 cacgtcgcag ttcatttccg caccgcgcaa tgcggcggca gtgtcggtcg tgaagaacgg
     1381 gttaccggta ccggcggcaa aaatcacgac tttgccttct tccaaatatt ggatggcttt
     1441 aggacgggcg taagtttcag cgatttgctg catagacagt gcggattgta cgcgcgcttt
     1501 gatgcctaaa gtttcaaatg cgtctttgag tgccaacgcg ttcatcacgg tcgccatcat
     1561 gcccatgtag tcggcggtgg cgcgatccat gctgcctgct tgggcggata cgccccggaa
     1621 aatattgccg ccgccgacaa caataccgac ttgcacgccc attttaacga cttcggcaat
     1681 ttcgccgacg gtttggacga tggtgtcgtg attgatgccg aacggatcgg aacccatcag
     1741 ggattcgccg gagagtttca gtaatacgcg tttgtatttg atttgctgtg tcatgggata
     1801 ccttgctttc ttgaatgtcg tcggtcggag ttgggccgtc tgaatttcca acggtcggaa
     1861 tattaatgat agcgtgtgtc ggtttattat tgttttgttt taagacggtc tgaatattcg
     1921 gtcgtcttaa aataggtacc gcaaacggtt ttctcaaaaa aaagcaccct gattcgtttg
     1981 gaatccaggt gctttctttt tcataagtgc tttacacttt ggcggctgcg gctacttcgg
     2041 ctgcgtagtc gacaacggct ttctcgatac cgtcgcctac tttgtagcgg acgaagctga
     2101 ccacttcagt gccgttttct ttggcgaatt gggcaacagt ttgatcgggg ttcatcacga
     2161 atgcttggcc gttcagagtg atttcagcca agaatttgcg aatacggcct tcaaccattt
     2221 tagcggcgat gtcggcaggt ttgccggaag cgatggcttg ttcggtgtag atgtggcgtt
     2281 ctttttcaac ggtttcggca tctacttcgg cttcgcttac gcattgtggt ttagcggcaa
     2341 caatgtgcat accgattttg cgtgctacgt cttcagagcc tttgtactca accaatacgc
     2401 cttcggtcgc caatgcgccg tggatgtagg caaccagttg gttggcagtg tcgatcactt
     2461 ggaagcgacg gacagacatg ttctcgccca atttggcgat gatggctttg cgttctgctt
     2521 caaccagttc gctcagttct tcaacagaag ccggtttttt ctcggcggcg gttttcgcaa
     2581 cgaagttggc aaattctacg aagcccgcgt ctttcgcaac gaagtcggtt tcgcagttta
     2641 cttcgaccaa tgcgccgaca ttgccgttga tcgcgtaagc caatacgcct tcggcagcgg
     2701 tacggccggc cagtttaccg gctttcgcac cggatttgat acgcaggatt tcttcggctt
     2761 tgtcgaagtt gccttcggct tcaaccaagg cttttttgca ttccatcatg cccaggccgg
     2821 tagcggcgcg cagatcggca accatttttg cagtaatttc tgccattttg aatctcctag
     2881 atttttggga aatacggaaa ggattttccg cattcgggaa tcgtgtgccg cgaacggacg
     2941 ttcagacggc atatttgaga aaaggggcat aacgcccctc ttcggtttgc cggattactc
     3001 ggcagcggct tcttgggcag cggctacggt ttcttgcagc gcttggtttt tgccttccaa
     3061 aactgcgtca gcgatgccgc ggcagtacag gcggatggct ttggcggagt cgtcgttgcc
     3121 ggggatgacg tatttcacgc cgtcagggct gttgttggta tcgactacgg cgataacggg
     3181 gatgcccagt ttttcagcct cgaccagagt acctttttgg tagccggtat cgataacgaa
     3241 aatcgcgtcg ggcaggcctt tcatgttttt gataccgccc aaagaacgtt ccagtttttc
     3301 aacatcgcgt tgcatttcca gaatttcttt tttgctgaaa ccgctttcgg cagcattttc
     3361 caaggctgcg gttttttctt ccaggcgttt gatggattgc ttaacggttt tgtagttggt
     3421 cagcataccg cccaaccagc ggtaatcaac gaaaggcata ccggcacggg tcgcttcttc
     3481 gcggatgatg tcgcgggctt ggcgtttggt acctacgaac aatactgtac ctttgttggc
     3541 aaccaaacgg cgtacggctt cttgcgcgtc ttggaacatc ggcagggttt tttccaagtt
     3601 tacgatatgg attttgttgc gtgcgccgaa aatgtattgt gccattttcg ggttccagaa
     3661 acgggtttgg tggccgaagt ggacaccggc ttcaatcatc tgacgcatag taatttgaga
     3721 cattttgatt tccttgaaag ggttaagggc acacattcaa tcgcatagaa cttgataggc
     3781 gatgcctatt gtcaagcacc cttcggcgaa agtgggcata agttttaaaa aataaaagag
     3841 tgtttccgaa atggaaaact gacggattat agcggataag aggccgtctg aaaagggatt
     3901 cagggttttg tgccgctgtc tttttgccgt tgtgcacgct gctgttcgaa ttctgcctgt
     3961 tcctgctgct tcatcaagcg gttgcggcgg cggacggtta cgacgaagac cgtgaacacg
     4021 gtgggcagaa ccgcccaaaa aaccaaataa atcaaggcgc gcgcaatact cggctgcgcg
     4081 gcggaataca taacggcaac aaaaagatag ccgatggcga cgatgtgcca cattgtgtgc
     4141 gtcctgtttg aatgttaaca aaatcgttat aatcgcaaca ttctaccgca atcctgaaag
     4201 gccgtctgaa atgaggaacg aggaaaaacg cgccctgcgc cgcgaattgc gcgggcggcg
     4261 ttcgcaaatg gggcgggacg tgcgggcggc ggcaacggta aaaatcaacc acctgctcaa
     4321 acgttatatt aaaaaagggc ggaaaatcgg cgtgtattgg ccgatgggca aggaattgcg
     4381 tttggacggc tttgtccgcg cggcgcaaaa acgcggtgta aaactttatc tgccttatat
     4441 cgaaccgcgt tcgcggcgga tgtggtttac gccgtatcct gaaagcggaa tggaacggga
     4501 acgcatacgg ggcagggcga ggttgaacgt gccgcagttt gcagggcgca aaatccgcgt
     4561 gcacggtttg tcggtattgc tcgttccgct tgtcggcata gaccgcgaag gctaccgttt
     4621 ggggcaggca ggcggctatt acgatgcgac gcttgcagcg atgaaatacc gtttgcaggc
     4681 aaaaaccgtg ggcgtgggct ttgcctgcca gttggtggac agactgtcgg tcgaggcgca
     4741 cgacctgccg ctggacggtt ttgtgtcgga ggcggggata ttgtgctttt agccgttcag
     4801 acggcatttc ggtcggaaat gccgtctgaa ggtggaatat tgggagaaaa ggatgtcgga
     4861 aaagataata ctgccggtct tgtcgttggg tggcgtacgg ctcgaaccgt tggatgtgca
     4921 tcatgaaacg ggtttgcgcg aggcggtttg cgatggggaa gtttggaagc tgggagtaac
     4981 gtccgcgccc catccggata gggtggcaga ctatatcggg acagcattgg caacgcgttt
     5041 ggcgtttgcg gttgtcgatg aagaggcggg cagggtggtc gggacaacgg cgtattatca
     5101 ttttgaaccg cagattccgc gtttggatat tggattcaca tggtatgcgg catcggcacg
     5161 gcggacacgg ataaacacct gttgcaaaat catgctgttg gattacgcat ttgatgtttt
     5221 ggcttgccgt tgcgtgggat ggcgcacgga tattctgaac cttgcctcgc aacgtgccat
     5281 cgaacggctg ggtgcggaaa aagacggcgt gctgcgtatg catatgctcc gtaaagacgg
     5341 cagtgtgcgc gatacggtcg tgtacagtat gctgagggaa gattggtgca aaaacaggga
     5401 aatcctgacc ggcaggttgg cagggtatgg cgtgcaggtg tgaggcagta ttttcaaatt
     5461 tatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttagctcaaa gagaacgatt
     5521 ctctaaggtg ctgaagcacc aagtgaatcg gttctgtact atttgtactg tctgcggctt
     5581 cgtcgccttg tcctgatttt tgttaatcca ctataaaaaa cttaacgcaa tatgctgaca
     5641 atcttttaaa aattaatata taaacaattt tgatttttta tctattaaaa atttgatata
     5701 tatagataaa taactatttt tatttttcat ttcgcagttt cccgacttgg cgggcgacag
     5761 aaaaactttg catttcccag taaaatccac tacattcacg ataacatatt acaagcccct
     5821 gaagcggtgt cggaagccat acttgcaacg cattccaacc ccgaaacagc atactcggac
     5881 acacaccatt gtcggttttc cctgcttttg cgattcaggc gtttgtagta cggcaaataa
     5941 ccataagggc agaagatgcc tgttttttta ggagtaaacc atggctgaag cgacagacgt
     6001 tgtcttggtg ggcggcggca ttatgagcgc gactttgggc gttttgctca aagaactcga
     6061 accgtcttgg gaaatcaccc tgattgaacg cttggaagat gtggcgttgg aatcgtcaaa
     6121 cgcgtggaac aacgccggca cggggcattc cgcgctgtgc gaattgaact atgcgccgtt
     6181 gggtgcaaat gggattatcg atccggcgcg cgccctcaat attgccgaac agtttcatgt
     6241 cagccgccag ttttgggcga cgctggtcgc ggaaggcaag ttggaagaca attccttcat
     6301 caatgccgtg ccgcatatgt ctttggtgat gaatgaagac cattgttctt atcttcaaaa
     6361 acgttatgac gcgtttaaaa cccaaaaact ttttgaaaat atggaatttt ccaccgatcg
     6421 gaacaaaatt tccgattggg ctccgctgat gatgcgcggc cgggacgaaa accaacccgt
     6481 cgccgccaac tactccgccg aaggtacgga tgtcgatttc ggacggctga cgcgccaaat
     6541 ggtgaaatat ttgcagggca agggcgtaaa aaccgagttc aaccgccacg tcgaagacat
     6601 caaacgcgaa tccgacggcg cgtgggtgct caaaaccgcc gatacccgca accccgacgg
     6661 gcagctcacc ctccgtaccc gcttcctctt cctcggcgcg ggcggcggcg cgctgaccct
     6721 gctgcaaaaa tccggcatcc ccgaaggcaa aggctacggc ggcttccccg tgtccggcct
     6781 gttcttccgc aacagcaacc ccgaaactgc cgaacaacac aacgccaaag tgtacgggca
     6841 ggcttccgtc ggcgcgccgc cgatgtccgt cccgcacctc gacacacgca acgtggacgg
     6901 caaacgccac cttatgttcg gcccttacgc aggcttccgt tccaacttcc tcaagcaagg
     6961 ctcgcttatg gatttgccgc tgtccatcca tatggacaac ctctatccta tgctgcgcgc
     7021 cggctgggcg aatatgccgc tgaccaaata cctgctgggc gaattgcgta aaaccaaaga
     7081 agaacgcttc gcctccctgc tggaatacta ccccgaggca aaccccgacg actgggaact
     7141 catcaccgca gggcaacgcg tccaaatcat taaaaaagac tccgaaaaag gcggcgtgct
     7201 ccagtttggt acggagattg tcgcccacgc cgacggctcg ctcgccgcat tgctgggcgc
     7261 gtcgccgggc gcatcgaccg ctgtgccgct gatgatccgg ctgatgcacc aatgcttccc
     7321 cgagcgcgcc ccgtcttggg aaggccgtct gaaagagctg gtaccgggtt acggcatcaa
     7381 gttgaacgaa aaccctgaaa gggcggatga aattatcgcc tataccgcga aagtgttgga
     7441 tatttaagcc gaatctttca agaaaagccg tacccccata tggtgttgac gccgtccctg
     7501 ccagttatgc ccttcatccg gcagggcagg ttttgataca cggggcaggt aatcgttttt
     7561 tcccttttgc gaataatgga acggtcaaaa aaacagcgtt ttcatttctg aaaacgctgt
     7621 tttttgttgg cttgttaacg tggggaacag tctttgaaga cgatttggtt gtcaggtgcg
     7681 gtaatcataa taggctgttt gcgataggat ttgccatcca tcacgccggt acccaaaaca
     7741 taaccgcctt ctttgccgta gaatgtttcc acattgtcgg atttgtccaa attgacaggc
     7801 atttgcacac gtttgccgtt gatgacggcg gaagcgtatg tggtcaggcc ctgtttgtta
     7861 aagccgtagg ttactttgac ttttttacct tgctggcaga cgtagctgac ggtttttttg
     7921 gcaacggtgg ggttgttcgt gccggcagca gcagccatac tgctgagcgc gattgcggaa
     7981 gacaggattg cggtggtcag aagtttcatt tttattcctt tggatggatg tggagtttgc
     8041 ccgtttcgga cggtttgaca ttctaatgtc ggcgtatcct gaaataaaca ggaaattgga
     8101 taaagtttgt aaaaaggcgg gttcgggcgg ttcggcggtt tgaggcaggg gcggcaggcg
     8161 ggatacggcg acctgtccgg tgtttcagac ggcattgttt gcgtttttcg gaaatgaaaa
     8221 actgtcgggc atccggaggt tgcggtttaa ctttgttttt ttctttcgga cttggtctgt
     8281 ttggacaact cggcatacag gtcgggatgg tgttttttca acagggcggc gatttccata
     8341 tcttttttgt tgactttcgc caaatcgatg cattcgatat ggacgaggcg gagcagttcg
     8401 cgtacgggtt tgggcatttt gcggccggtt tcgtagcgcg agcctccaga ctgggtaacg
     8461 ccgaccttgc cccagaagtc tatctggttc agcccggtct ttttacggat ggctttaaca
     8521 tcttctatat tttcaaatat tttcataaaa cttcctttaa gtttgtctgg ttgcggggca
     8581 aattccgatt tgctttgttt tcgtaatgtg ccgggtcaag cctgttttta tattatatat
     8641 catatctgcc gtttctttca gacggcattg tttgttttta tggggcggat acgtccggtt
     8701 tcagggtttg ccggaggcgg ggctgacggt gaggatttcg tagccggttt cggtcaccaa
     8761 gacttcgtgt tcccattggg cggagaggga gcggtctttg gtaaccaccg tccagccgtc
     8821 gttgaggata cgcaggtggc gtttgccttg gttgatcatc ggttcgacgg taaaaatcat
     8881 acccggtttt agaacggggc cctgtccttt ttttccgtag tgcaacactt gcggggcttc
     8941 gtggaaaccg cgcccgatgc cgtgtccgca gaattcctgt acgacggaat agccggcgtt
     9001 ttcggcaacc tgttggcagg cgtaacctac gtcgcccagt gtcgcgccgg gtttgacggc
     9061 ttctatgccc gccatcatgg aggcgtgggt tacgtcgatc aggcgttggg cgatggggga
     9121 gactttgccg acggtaaaca tacggctgga gtcgccgtgg aagccgtctt ttttgatggt
     9181 gaggtcgatg ttgataatgt cgccttcttt gagcggcttg tcgtcgggaa tgccgtggca
     9241 gatgacgtgg ttgacggagg tgcagcagga tttggggtag ggcgggttgc cgtagtgcag
     9301 gggggcggga tagccgcctt ggacgttgac gtggtagtcg taaacgagtt tgtcgatttc
     9361 gtcggtggtt acgccgggtt tgacgaattg tccgatgtag tcgagggctt cggcgacgag
     9421 tttgcccagc tcgcgcattt tttcgatttc ttcgggggtt ttgatgatga tgccgttcat
     9481 aatgcttcct tggattgcgg gtgtgcctgc cgtgccgggt cagaagaggt tgtcgagaat
     9541 ttctttatgg ggtttgggcg gcgtgttttt cggggtgtcc ggtttggtat ggttttcttt
     9601 gggcgtttct ttttctttgg gtgtttcttt gggctgcgcc tgtttttcgg gtgtttcttt
     9661 tcggggttcg ggacggggga cgtttgtggg cagtacgggg gtttctttga gtgtgttttc
     9721 agcttgtgtt ccggcgtcgt ccggctgcct gtcggtttga gctgtgtcgg caggttgcgg
     9781 tttgccccgg tttttcttgg gtgcggcagg ggacggcagt ccgctgccgc tttcgtctgt
     9841 gccgtccggc tgtgcgggtt cggatgaggc ggcgtggtgg tgttcgggtt gggcggcatc
     9901 ttgtccgact acgccgtttg gcagccagaa ttcggtttcg cgggggctgt cggtgtgttg
     9961 cggttcggtt tggagggttt tgccgccgtt cgggttgaat gtgctgacga tgcctattgc
    10021 caatgcgccg atggcggtga tacagatgag caatacgacg cgtatcagga gtttgggggt
    10081 cagcctgaag ggtttgttcg gtttttttgc cattttgatt gtgcttttgg ggtgtcgggc
    10141 aatgccgtct gaaggcggtt cagacggcat tgccgagtca gcgttggacg tagttttggt
    10201 agagggtgat gactttttgt acgccgacgg tggtgctgac tttttgggta atctgcgcct
    10261 gttcttcggg ggtgaggatg cccataacgt aggttacgtt gccgtaggta acgattttga
    10321 cgcgcgcctg tgtggcgggg ctgatgccca acagcgtggc gcggactttg gatgtgttcc
    10381 aagtgtcgcc ggcgatgtcg ccggcagtgc gcggcaggga ggcgacggta atgtagttgt
    10441 atacgccttc ggcggcctgt tcggaacgtg caatctgacc gacgaactgt ttctcgcctt
    10501 cggtggcgac ttgtccgagc agcagcaggt ggcggttgta gccgacaacg gagatttggg
    10561 gcgtgtagcc tttggtttgg ttgttttggc gcagatagga gcgggcggtg gtttcgatac
    10621 gcaacgccat aacgttgtcg tcggtttgcg cgccggtggt tcggcggtcg acggcggatt
    10681 tcgcgccgac ggcggcgctt ccgattactg cgctgacgca gccgctaagg gcaaggctga
    10741 aaatggcggc aatcagggtg cggacggtgt gcggtttggg tttcatcggg tgcttccttt
    10801 cttgggcgtt tcagacggca ttgctttgcg ccatgccgtc tgaaaagggt tacattcctt
    10861 ccagcagtac ggagtcgata cagtcgcaca tggcgtgtat cagcaggatg tggttttcct
    10921 gaatacgggc agttcgagga tagggaacgt tgagtaaaac gtcggtgtct ttgagcatgg
    10981 cggcgatttt gccgccgtcg cggccggtca gggcgatgac gtgcatatcg cgttcgtgtg
    11041 cggctttgac ggcttcgatg acgttggcgg aattgccgga agtggaaatg ccgaccaaga
    11101 cgtcgcctgc acgtccgagg gcgcgtacct gtttgctgaa gacgtggttg aagccgtagt
    11161 cgttaccgat ggcggtcagc gcggaggtgt ctgtggtcag ggcgacggcg gcgagttcca
    11221 tgcgctcttt ttcaaagcgt ccggtcattt cggcggcgaa gtgctgggca tcggcggccg
    11281 aaccgccgtt gccgcacgcc agaatcttgc cgtcgttcat caggcattgc agcatcagct
    11341 cggcagcctg tacggtcggc tcgaccagta ctttttcggc ttcctgtttg gcacggatgc
    11401 tttcggcaaa atgggcggca acgcgttctt gtaatgtcgt catatcgtca acctgtaata
    11461 ttctgtatcc actcgggcgg gcggtttcct tcaatcagta ccgcatcgag gcggcacggt
    11521 acgtttgtca gcctgttctg ttgcagataa tactctacac ttcgttgcag tttcaataat
    11581 ttggatgggg aaatgctgta tgcgacaccg ccgaattgcc gatttttgcg gtattttact
    11641 tcaacaaaca gaatcatacc gccgtttttg acaatcaggt cgatttcgcc gtaggcgcag
    11701 tgccagttgc gggcaagcag tgtgcagcct tgggattgga ggaaggcaag cgcggcatct
    11761 tcccctgcct cgccctgttt gtggtttagg cgcataatcg gtttaacctt tataatgttt
    11821 gtttttcaga cggcattctt atgtttcaga aacatttgca gaaagcctcc gacagcgtcg
    11881 tcggagggac attatacgtg gttgccacgc ccatcggcaa tttggcggac attaccctgc
    11941 gcgctttggc ggtattgcaa aaggcggaca tcatctgtgc cgaagatacg cgcgttaccg
    12001 cgcagctttt gagcgcgtac ggcattcagg gcaaacttgt cagcgtgcgc gaacacaacg
    12061 aacggcagat ggcggacaag attgtcggct atctttcaga cggcatggtt gtggcacagg
    12121 tttccgatgc gggtacgccg gccgtgtgcg acccgggtgc caaactcgcc ggccgcgtgc
    12181 gtgaggccgg gtttaaagtc gttcccgtcg tgggcgcaag cgcggtgatg gcggctttga
    12241 gcgtggccgg tgtggaagga tccgattttt atttcaacgg ttttgtaccg ccgaaatcgg
    12301 gagaacgcag gaaactgttt gccaaatggg tgcgggcggc atttcctatc gtcatgtttg
    12361 aaacgccaca ccgaatcggg gcaacgcttg ccgatatggt ggaattgttc tccgaacgcc
    12421 gattaatgct ggcgcgcgaa attacgaaaa cgtttgaaac gttcttaagc ggcacggttg
    12481 gggaaattca gacggcattg tctgccgacg gcaaccaatc gcgcggtgag atggtgttgg
    12541 tgctttatcc ggcgcaggat gaaaaacacg aaggcttgtc cgagtccgcg caaaacatca
    12601 tgaaaatcct cacagccgag ctgccgacca aacaggcggc ggagcttgcc gccaaaatca
    12661 cgggcgaggg aaaaaaagct ttgtacaatc tggcactgtc ttggaaaaac aaatagtgcc
    12721 ggacgagccg gcaccatagt gtgaacggaa tcagcggaca cgacccgtca tgcggcacag
    12781 aacatctcta ccctgaacac ggtgggcgac gaccatggcg acgtgtccgg cgacggcggc
    12841 aaacagcagc aagcccaaat tgccgtggaa cgtgttgccc aagtttgcca tccactcgat
    12901 tttttccggc gaacccttgc atcacttcaa cgccgaacac tttcaacggg ccgcggccgc
    12961 tgccgtattg gcggatcatg ccgataacgg gaacagcaag catgagcaga tacagaatgc
    13021 cgtggcctgc cgccgcagcc ttgctgtcgc tttgcggacg cttggcgcgg ttggcaaccg
    13081 cccacacgat gcgcaatgta atcaccgtca gcgtaaggaa accgaaagat ttgtgcaggc
    13141 cgaacaggct gcccacccat tccgcctctt cgtatagtgg attaacaaaa accagtacgg
    13201 cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
    13261 tttgttaatc cactataaat cgtccacagg acaatggtgg tcagaatgcc gatgaagccg
    13321 gcaacggtca gccagtgcag cgcgcgtgtc aacgggtcgt aaacggcagg ttggtttgtg
    13381 ttcataaaaa act
//
LOCUS       DAWFIE010000058        12879 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWFIE010000058 DAWFIE010000000
VERSION     DAWFIE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12879
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.058"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(58..1368)
                     /locus_tag="WGQ71_001494"
     CDS             complement(58..1368)
                     /locus_tag="WGQ71_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ0707483.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGYFRIQGAIIITILTITVIASLMGLN
                     EFHGVVGEVPSIAPTFMQMDFKGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(1507..2685)
                     /gene="ftsZ"
                     /locus_tag="WGQ71_001495"
     CDS             complement(1507..2685)
                     /gene="ftsZ"
                     /locus_tag="WGQ71_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ0707484.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(2804..4048)
                     /gene="ftsA"
                     /locus_tag="WGQ71_001496"
     CDS             complement(2804..4048)
                     /gene="ftsA"
                     /locus_tag="WGQ71_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ0707485.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(4134..4862)
                     /locus_tag="WGQ71_001497"
     CDS             complement(4134..4862)
                     /locus_tag="WGQ71_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ0707486.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKTLGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(4852..5766)
                     /locus_tag="WGQ71_001498"
     CDS             complement(4852..5766)
                     /locus_tag="WGQ71_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ0707487.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAESCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(5879..7288)
                     /locus_tag="WGQ71_001499"
     CDS             complement(5879..7288)
                     /locus_tag="WGQ71_001499"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ0707488.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(7442..8509)
                     /gene="murG"
                     /locus_tag="WGQ71_001500"
     CDS             complement(7442..8509)
                     /gene="murG"
                     /locus_tag="WGQ71_001500"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ0707489.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFEGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARLQMYHQ
                     SGRNKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(8513..9838)
                     /locus_tag="WGQ71_001501"
     CDS             complement(8513..9838)
                     /locus_tag="WGQ71_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ0707490.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(9964..11301)
                     /locus_tag="WGQ71_001502"
     CDS             complement(9964..11301)
                     /locus_tag="WGQ71_001502"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ0707491.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGVEVAAYDAELKPERVA
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKDKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEYEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     ATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDALQIRRDLDGCDLN
                     MTDCATLEEAVQKAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL"
     gene            complement(11386..11658)
                     /locus_tag="WGQ71_001503"
     CDS             complement(11386..11658)
                     /locus_tag="WGQ71_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ0707492.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRQDRALAQEWYGKACQNRDQDGCGHYQRLKAGY"
     gene            complement(11773..12855)
                     /locus_tag="WGQ71_001504"
     CDS             complement(11773..12855)
                     /locus_tag="WGQ71_001504"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ0707493.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         3056 a         3700 c         3258 g         2865 t
ORIGIN      
        1 tggcaaacaa aatgccgtct gaaaaccttt cagacggcat ttttaatatc gaatcaatca
       61 gcccaaatac cagaatttca gtgcccacaa tacggcaaca atccatacca taggcggaac
      121 gtctttggtg cggcggcata aaagtttaac cacggcatag ctgatgaagc cgaaggcgat
      181 gccgtctgca atcgaatagg taaacggcat gaagacaatg gtcaggaatg cgggtgcggc
      241 ttccgtcata tcgtcccaat caatatccct cgcactgcgg agcatctgcg tgccgacata
      301 aagcagggcg ggcgcggtgg caaaagcggg aacacttttc gccaaaggtg aaaacatcag
      361 gcaggcgagc atcaatacgc cgacggtaac cgccgtcagg ccggtccgtc cgcctgccga
      421 tacgcccgcc gcgctttcca cataaggcgt ggtggaagaa gtacccaaag ccgcacctgc
      481 cacaatggcg gtagagtctg caagcagtgc gcgtttcagg cggggcagct taccgtccac
      541 cagcagtccg gcacggtggg atatgccgac cagcgttccg gtactgtcaa atagatcgac
      601 caagaagaag acgaaaatca cactgaccat actgacggta aacagacctt taaaatccat
      661 ctgcataaag gtcggcgcaa tgctcggtac ttcgccgacc acgccgtgaa actcgttcaa
      721 acccatcagg ctggcaatga cggtaatcgt caaaatggta atgatgattg caccttgaat
      781 gcggaagtat cccaaaacaa ccaccatggc aaaaccgaac agtgccaaca acgcagacgg
      841 ttgatgaata tcgcccaaac cgaccaaggt tgccggattg gcaacgataa tgcctgcgcc
      901 tttcagggaa atcagtgcca aaaacaaacc gataccggca gcaatcgaca ttttcaaacc
      961 cataggcagt gcgttgacca gcatttccct gactttaaaa aagctgaaca ggataaaaat
     1021 cagaccggag atgaacaccg cacccaacgc aacctgccaa ggcacgccca tacccttaac
     1081 gacggcaaag gtgaaatagg cattcagccc catccccggt gcgagtgcaa tcggatagtt
     1141 gccgacaaaa cccataacaa aacagccgat ggcagacgcg atacaggtag cgacgaatac
     1201 cgcccccata tccatgccgg tctcgcccaa aatcagaggg ttgacgataa cgatgtagca
     1261 catcgtcaaa aaagttgtca aacccgccat caactcggta cgcaccgtcg taccgtttgc
     1321 cttcagctta aaaatcccgt ccaacagtgt ttgttttgaa gtgtccatat ctgaaagtct
     1381 gtatcaagtg gaaaaatctg aatccgccga ccgcaaaccg tacgaaattg caacatcttg
     1441 aaaaacatac ctcccaaacc ggacaaaccg attcgggagg cagcaggttt acgggcaaac
     1501 agcacattat ttgtctgaat tgtgttgacg acgcaaaatc gcagggattt caaagtcgtc
     1561 aagtacggac tgattgtcga aatccgcagc ggtaaggttc atcgtgcgga taccgcgatt
     1621 ggtgcggatc ataccttcga cattgtggct ttgctcctgt ttggacggag caaccgcttc
     1681 tacctccctt gccggaacaa aatcgaccgc gcctttttct ttcagaccgg tagcgataat
     1741 ggtaatccgg atggcatctt cgctcatggt ctcgtcttca gccgcaccga atttgcattc
     1801 caaatcggga tgcgcgcttt ggttgacgat tttcatgact tcggacaact cggacatttt
     1861 caagcaaccc ggagcagtcg taatattgac cagcacaccg cgcgctccgt ccaaggttac
     1921 atcgtccagc agcggactgg aaatggcctg gtcggtcgcc atacgcgcac ggtcgatacc
     1981 ttgggcataa cccgaaccca tcatagcgat accgcggttg ctcatcacgg ttttcacgtc
     2041 ggcaaagtcg aggttgatga tttcgctcgg gcaagttacc acttcggaaa tgcctgcgac
     2101 cgcatcgcgc aatacattgt cggcggcacg gaaggcttcg cgcatcgtta cgtcttcacc
     2161 caatgcagtc atcagtttgt cgttcgggat gatgatcagc gaatcgacgt gttctttcaa
     2221 ctgttccaac cctgcctgtg cgacatggac gcgcttacct tcatatgcga acggtcgggt
     2281 aaccacggca acggtcagaa tgcccaaaga cttggcaatc tcagcaacaa ccggcgcgga
     2341 accggtaccg gtaccgccgc ccataccggt cgtgataaac agcatattcg caccgcgaat
     2401 ggcttcttca atggcttccc ggtcttcctg ggctgccgca cggccgatat cgggattcgc
     2461 gcccgcgccc aaaccgcgtg tcagattcgt acccaactgg attctcttcg ccgcatggtt
     2521 ttttgccaga gactgcgcat ccgtattggc actgataaac tccacaccgc gcacattgtt
     2581 ggcaaccatg ttattgattg cattgcaacc gccgccgccc aagccgatta ctttaatcac
     2641 cgcagggctg actgccgatt ctgccacgtc gtaaacaaat tccattcaaa aactcctgct
     2701 cgccccattc agaggacggt ttaaataaat tattattcat tatataagaa gtatcttgct
     2761 gctggcaaaa tacttctcac ctgtcaaacg gcaatccacc tgttcagaag ctgttttcaa
     2821 tccaccgttt caatcttgcc aacaaaccgc cgcccccttc cctctcttgc actgcaccgt
     2881 tttccggctg cggcaagttt ccttccagct tgcatgctgc atgaagcagc ccgatagcgg
     2941 tagaaaaacg cggtgtgcgg acgcggtcgg acaaaccgcc catttcttgg ggtgcaccgg
     3001 tgcgtacagg caaatcgaag attttttcgg caaattccac aatcccggtc atcatggaca
     3061 caccgccggt cagaacgata cccgcattca gcacttcttt ggggaaaccc gatttttgca
     3121 gctcgcccag cactacgcca aaaatctcct gaatccgtgc actgatgatt gctgccagaa
     3181 ccttactgga aacctgacgc gatgtccggt cacccacgcc cggaacttca atcatctcac
     3241 ccaagccttc cgtatcgcat gatgccacgc cataatggat tttaatgtac tcggcggcat
     3301 cgagaggtgt tctcaacgat ttggacaaat ctttggtaat cagattacca ccggccggaa
     3361 tgacggacgt atggcggatg gcaccgttca tataaacggc aatatcggtc gttccgccac
     3421 caatgtcgat gacgcatacg ccgaggtctt tttcatcttc agtcagcacc gcctgcccgc
     3481 ttgccaacgg ctgaagcatg atttgatcgc ttttcaagcc gcaccgctcg atacattttt
     3541 ggacattctg cactgccgta cttgcaccgg taatgatgtg cacccgcgta tccaaacgca
     3601 caccgctcat accgatgggc tccctcacgc caagttgggt gtcgataatg tagtcttgaa
     3661 ccacagcatc gagaattttt tgatcgggcg ggatattgat tgcctttgcc gtttcaatgg
     3721 cgcgatcgat gtctgcctgc gtgacttccc cgtctttaat tttaaccaca ccttgcgaat
     3781 taagactgcg gatgtggttg cctgcgatac ctgtggtaac gtgggtaatt ttggtatccg
     3841 ccatcagctc ggcatcattg accgcctgcc tgatggcttg gacggtggca tcgatattgg
     3901 ttaccatgcc cgcgcgcaag ccccgtgaag gagcctgccc caaaccgacg atgttgattt
     3961 tgtcgtcatc ttgaacttcc ccgatcagtg cgaggacttt agacgtaccg atatccagta
     4021 cgctgatgta tctttgctgc tgttccattg ttcgtctgct cttaaaactg attgaaattt
     4081 gcgtcgcacc gtttcagacg gcacggccgt aatctgtccg atacctgttc ccactattct
     4141 tcggattctt tttcgggtaa accgtcggaa gcatagcgga ctgaaaatcc gtccttatac
     4201 ctcatatcca cataggataa ccgattttta tttttacgca acagatgctg ccacgcttcg
     4261 gtaaaaagcc ggaggcgttt catctcgttt tcccgtccga gcctgacggt gatgccgttg
     4321 tccaaaacga caatccacgc cgaacgtgcc gtataggtca tctctttgat gcccaaaccc
     4381 tgttttgcca aaacagtcga aaattcgtca taacggcgga gcatttcggc agacgttcct
     4441 tccgcgcctc tgaataccgg cattccgggt ctgtccaagc gggcttcaaa aacattgcct
     4501 tcgccgtcca ccaaggcatg gtcgccccaa cgcgcgaccg gcttgcgctc ggtcaggacg
     4561 acctcaaccg tgtcgggaaa acggcggcgc accatgaccg acgcaatcca cggataccgg
     4621 cggtaggcct cctgtgcgcc attgatgtcc gtcctcaaaa tattcccatg gatgtattct
     4681 ttcgccaaac tgcccaatgt cttcttatcg gaataaacca ggttgccctt cagcgacacc
     4741 tgcttgacgg gcagatgatt cgaattgtaa aaccaaacca gcccggacgc agcaagcagc
     4801 atcgccatca tgacaagcag ccagcgcgtc agccgttcca tcgcttcggc attatcccac
     4861 atgtgcggtc ttcaaaattt caatacataa atcggcaaaa cccacgcccg taacggcagc
     4921 ggatttcggt actaaactat ggctcgtcat accgggcagg gtgttgattt ccaacagata
     4981 gagtttgccg tcggtatctt tgaggaaatc gacgcgcacg cagctttccg caccgattgc
     5041 ctgcgccccg cgaaccgcca gttcgcgcat caggctttct tcggcttcgg tcaaatcttc
     5101 cgaaggacat tgataaatgg tgtcgtcgcg gttgtacttg gcttcgtagt cgtaaaactc
     5161 ggttgcggga atgatgtgta tgccgggcag ccctttgccg ttcaggacgg ggcaggaata
     5221 ttcgccgccg ccgataaacc gttcggcaat gatttcgccc tgaaggtgtt tcaattcttc
     5281 gtaaacgctt ttcagacggc cttttccttt gacttttacc acgcctacgc tgctgccttc
     5341 ggccgccggt ttcacaaaca tcggcaggcc caatttttct tcgacggcat cgaaatcagt
     5401 gtcgtcgtgc aggacggcga actcgggaac gggcagcccc aatgcctgcc aaatcagttt
     5461 gcagcggtat ttgtccatgc cgatggcgga ggcggcgaca ccgctgccgg tatagggaat
     5521 gcccaacagt tccaacgcgc cttgaaccgc cccgtcttcg ccgtaagtac cgtgaaggat
     5581 gttgaatgcc gtctgaaaac cttgtgcctt caattcagac aatggggttt ccttgggatc
     5641 gaaggcgtat gcgtctatac ctttgctttt taaagcattc aaaatggcgg tgccgctgtc
     5701 cagcgagatt tctcgttcgc tggaaaaacc gcccatcaat acggccactt tgccaaaatt
     5761 ctgcattgtt tttgttcttt cctgattgct ttatgcttgt tgccagaggt cgtctgaaac
     5821 ctgatttgcg gtttcagacg acctttatat gatgttccgt ctgtcaggcg ggtgtgcctc
     5881 aaatctgttt cgacaattcc agcagcgcgg cggggacgcg gttgatgctt cccgcaccca
     5941 tattcaacac gatgtcgccg tcctgcaaaa cgttcaacag catttcgggc agatcggcaa
     6001 cgttttcgca gtaaatcggc tcgagtttgc ccaacacgcg gatggcgcgg gcaagagcgc
     6061 gggaatcggc ggcggcaatc ggctcttcac cggcggcata aacttcggtc agcaccagcg
     6121 cgtcaacggt attgaggact ttggtaaagt cttcaaacaa atcgcgcgtg cgggtatagc
     6181 ggtgcggctg gaaggcgagt accaaacgtt tttccggata cgcgccgcgt gcggcggcaa
     6241 gggtcgccgc catttcgacg gggtggtgtc cgtagtcgtc caccaagagc gcggtcccgc
     6301 cgtttggcaa cttgatgtcg ccgtattttt ggaagcggcg gccgacacct tcaaagccga
     6361 gcaagccttt ttggatcgct tcaaccgatg cgccgacttc cagcgccacg ccgatggctg
     6421 ccaatgcgtt cagcacgttg tgtctgccgg gcatattcag cacgacttca aacgacccct
     6481 gctcatgtcc tttcatttga acatggacgg tgaatttcat ttgcgcgccg acgttttcga
     6541 tgtcggtggc gtagatgtcg gcggtatcgt ccaaaccgta agtagcataa ggtttgctca
     6601 ctttgggcaa aatcgcgcgg acgtgttcgc tgtcaataca caaaaaggct ttgccgtaga
     6661 agggcatacg gtggatgaaa tcgataaacg cctgatgcag tttttcgacg ctgtgcccgt
     6721 aggtatccat atggtcttcg tcgatattgg taacgacgga cataatcggt gtcaggtgca
     6781 gaaaggatgc atccgactcg tcggcttcgg caacgatgta ttcgcctttg cccaagcggg
     6841 cgttggtgcc tgcggcgttg agtttgccgc cgataacgaa agtcgggtca agtcctgccg
     6901 cgccgaggat ggaggcggtc aggctggtgg tcgtggtttt gccgtgcgtg ccggcaatgg
     6961 cgatgccgtc acggaagcgc atcaactccg ccaacatcag ggcgcgcgga ataacgggaa
     7021 tttgctgctc caacgcagcg acaacttcgg gattttcttt tttgacggcg gtagaggtaa
     7081 cgacgacatc cgcaccgtta acgtgttctg cggtatggcc gggataaact tgaatgccca
     7141 ggctgcccaa atgctcggta gcggcatttc gcgcctgatc cgaaccggaa actttaaaac
     7201 ccaaattgtg caagacttcg gcgataccgc tcatgccgac gccgccgata ccgacaaaat
     7261 ggatgttggt cactcgattt ttcatcataa tgttgcgttc cggtggattt cgatgcgtaa
     7321 aggcgttatt ttaaagggct gaccgtttgc gcgccatagt tttctgacaa atatatagcg
     7381 gattgaaata aaaaacatcc atgccgtctg gacggcattt cagacggcat ggttcggcag
     7441 tttacgccgc acacgcaatc gcggcttcgg caacgtcgtc cgcgctgtgc ggcaatgcca
     7501 aagtacgggc gttttccgcc catttgagac atttttcgcg gttcaggctg ccgaggattt
     7561 cggcgagttt ttccgccgtc aactgggttt gcggcaacag caatcccgcc tccgcctgca
     7621 ccataaaacg cgcgttggcg gtttgatggt catcaacggc gtgaggatac ggcactaaca
     7681 acgcacccaa tcccgccgcc gtcaactcgg caatcgtcag cgcgccggca cggcaaatca
     7741 ccaaatcggc atcgcggtag gcggacacca tatcggtaat aaattccacg cattcggctt
     7801 tcacgcccag cgcgtcgtaa tccgcctgca agctgcccag cttgttgcgc cccgactggt
     7861 ggtacatctg cagacgcgca ttgtcgggca gcaaagccaa tgcctgcggt acggttttgt
     7921 tcaaaacgtc cgcgcccaaa ctgccgccga ccaccaaaat tttcagacgg ccttcacgcc
     7981 cttcaaagcg ttcggcaggc acgggcaggt tgctaatatc ggcgcggacg gggttgccga
     8041 ccaagccgcc ttcgtggctg aacgctttcg gaaaagcgta caacacccgc ttcgcccagc
     8101 gcgacaggtg gcggttggac aaacctgcca cggcgttttg ctcgtgaatc acaatcggca
     8161 cgcccaagag tttcgccgcc aaaccgccgg ggaaggtaac gaagccgccg aagccgatga
     8221 cgcactcgac acggtgtttg cggataatcc gctgcgcttc gcggacggtt tgatacaaag
     8281 taaacggcag catcagcttg cgtttgatgc cgttgccgcg cacgccttta atcgccagcg
     8341 tttccaagcg tatgccgtat tgcggcacga tacgctcttc catcgaatcc ttgctgccca
     8401 gccaaatcac atgatggccg cgcgcgcgca atgaatccgc caccgccagc gcggggaaaa
     8461 tatgtccgcc cgttccgccc gccatcagca taaaggtttt accgcccatg atttactcca
     8521 cccgataacc gcgcattttc cggcggtttt cataatctat acgcaacagc agcatcatgc
     8581 tgatcagcat gaaaaagact gacgaaccgc cataggacat caacggcagc gtcagacctt
     8641 tggtcggcaa agcaccgatg ttcacaccga tattgaagaa actttggata ccgatccaaa
     8701 tgccgatacc cgaagcgata taggcgttga aagtcaaacc caaatcgcgc gactgcttgc
     8761 cgatggaaaa cgcccgcacc accagccagc cgtaacagaa tatcagcacg cacataccga
     8821 agaaaccgaa ttcttcggcg atgatggcaa aaataaaatc ggtatgcgct tccggcagaa
     8881 agccgcgttt gctcaaactc gcacccaaac ccataccgaa ccactctccg cgcccgattg
     8941 ccatcagaga gtgggtaagc tggtagccgg caccctgcgg gtctttccac gggtccaaaa
     9001 atgccactac ccgctgcaca cggtagggag cggcggtaat catcagcacc atcccgccca
     9061 agacgctgcc taccaggacg aaaaaatatt tccacggcaa tcctgccaaa aacagcattc
     9121 caacggcaat gacggtaatg acgacaaacg aaccgaaatc cggctgtacc attatcagca
     9181 ccaaaccgaa cgccaccagc ataatcggca ggatgatcgc ccggaaacgg ccgtacattt
     9241 ctaatgtttc acgacgtgcc tgcggattgg tggcggacat gatcagattg gccgtccccc
     9301 gccaaatcga ctgccaaccc aaactttcca tgctgcgcaa cacttcttca cggcgcgtga
     9361 acaggcttgc caaataaagg atgaccgcca gcttgaacag ctcggtcggc tggaaattca
     9421 acggacccaa aggtatccaa cgggtcgcgc cattgatttc gcgcccggca atcaatacga
     9481 ctaccagcaa caggccggat agggcaaaaa tccacggcac aagccgccgc catgtcctca
     9541 tcctgcaaag aaaccataaa aaaccgctcg ctatcaagcc ggcaacgacg aaccccgcct
     9601 gtctggtcaa atagaaaaac tgatcgccgc cttcttttga tgccaaatac acagaagccg
     9661 aataaatcat cagcaggctg aacgccgtca tcagcaccac catccacaaa agcggcgcgt
     9721 cgaatttcct gccgtcgcgc acaatcggcc tgtcgagcag cagagtgtgg acaccgtcgc
     9781 ccacttttac caatacttcc gaaatcttca aaaaaaccac ctgccagtct gtttgcacct
     9841 gccgcaaagg gcaaaaattt cagacggcag acaatgccgt ccgaacatac gatacatccc
     9901 aaatcggtat tctaaatctt tacttgccgc ccaacaatga cggcgtttgc gtttcagacg
     9961 gtatcacaaa gccttaaacg ccccgataaa cacttccgaa cggtgcgcat agcctttaaa
    10021 catatcgaag ctcgcgcagg cggggctgag cagcacgata tcgcccgctt cggcttgggc
    10081 atatgccttc tgaaccgctt cttccaaagt ggcgcagtcg gtcatattca gatcgcagcc
    10141 gtccaaatcg cggcggattt gcagcgcatc gacaccaatc aggaacacgc cttttgcctt
    10201 gccggcaagc gcgtcgcgca ggggcgtgaa gtcctgccct ttgcccatgc cgcccaaaat
    10261 gacgaatagc ggattttgca aaccggcaat cgcggcggct gtcgcgccga cgttcgtgcc
    10321 tttgctgtcg tcgatgaaca ccacgccatt tttctcgccg attttttcca cgcggtgcgg
    10381 caggccttga aaggttttga cgtgttcgag caatgcttcg cgcggcaaac cgacggcttc
    10441 gcacaaggca acggcagcca tcacgttagt ggcgttgtgc aagccttgca gcggaatatc
    10501 ttgcgtggca atcaaatctt cattgccttg tttcaggcga cctgtctcac gttccaacca
    10561 aaaatcggct tcgtattcca acgaaaacca tttcacctcg cgcccggcgc gcttcatcgc
    10621 acgacagaac gcatcgtccg cattcaaaac ctgcacgcca tcgccacgga aaatcttatc
    10681 cttggtatgc gcatagtcga gcaagtcgtc gtagcggtcg agatggtctt cggaaatgtt
    10741 cagcaccgtc gccgcagtcg ggcgcaggct ttcggtgttt tccagttgga agctggaaag
    10801 ctccaacacc cacacgtccg cctttttgcc ttcgcgctgc cattccgcct ccaaaaccgg
    10861 cgtgccgata ttgcccgcga taacggtatc cagcccgcac ttgatgcaga gatagccgac
    10921 caggctcgtt accgtggttt tgccgttgct gccggtaatc gcaatcacct tgtcgcctcg
    10981 gcggttcaca atatccgcca gcaattcgat atcacccaac acgcgcccgc cgttttgctt
    11041 gaacgcctcg atatccggct gccgctcgct gatgccgggg ctgagcgcca gaatatcaaa
    11101 accgttgtcc agcgcatctt tcagacggcc tgtgtaaaac accagcccgt caaacatctt
    11161 accgatttgc gccacgcgct cgggcttcaa ctccgcatca taagcggcaa cctccacgcc
    11221 gtttttgcgc aggtaggcaa tcatggaaat acccgtgccg ccaagtccgg cgacgaggat
    11281 ttttttgttt tggaaagtca ttttggtttg tcctaaaaca aatcatattg ggcaggaaga
    11341 tgtctgctcc tgctcaagcc gctttcagac ggcatcgcga gctgttcaat aacccgcttt
    11401 caggcgttgg taatggccgc agccgtcttg gtctctgttt tgacaagcct taccgtacca
    11461 ttcttgggca agggcgcggt cttggcgcac gccgcgtcct ttgtaataca ttgcgcccaa
    11521 attgtattgg gctgctgcat ttccctgtgc tgccgcctgc aagttttccc gaaaatccga
    11581 tacgtcatcc gcccacaccg cttggttcaa gcccaaggca atcagggcgg cggcaagcca
    11641 tttgactgtc tgtttcatgg ttttacttct gttttagtat aagccgccgt taacgatagg
    11701 gctgggcgga ttgtcgccgc aggtttattg cgcgttcaaa tgccgtctga aagatgttca
    11761 gacggcatag gttcagcgga ttttgagggt actcaagccg atcaatacca agacgatggt
    11821 gataatccaa aagcggacga caacttgggt ttctttccag cctttttgtt cgtagtggtg
    11881 gtggatgggt gccatcagga aaatgcgttt tttggttttc ttataccagc cgacctgaag
    11941 cataacggac acagcttcga cgacgaacag accgcccata atcactaaaa caaactcttg
    12001 gcggacgatg acggcaacgg taccgagcgc ggcacccaat gccaatgcgc cgacatcgcc
    12061 cataaagact tgcgcgggat aggcgttaaa ccacaagaaa ccgaggcacg cgccgcacat
    12121 ggcggtgcag aatatagcga cttcgttcgc gccggcgaca tagggaagct ggaggtattg
    12181 ggaaaattgg tagtgtccgc tgacgtaggc gaaaatggcg aggccggcgg caacgaggac
    12241 gacggggaag gtcgcaaggc cgtccaagcc gtcggtgagg ttgacggcat tggatgtgcc
    12301 gacgatggtc aggtaagaca acaccaaaaa gccgaccacg cccagcggca gggcgatttg
    12361 tttgaagaac gggacaatca aaatattgtt ggcggaattg gcggcaaggt aaaacaatgc
    12421 caaaccggcg ataatggcaa cgcttgactg ccacaccatt ttgaatttgg cggacacgcc
    12481 gttggggtct ttatagacga ctttgcgcca gtcgtcgtaa aaaccgagtg cgcccgtggc
    12541 gagcaatacg cccaagagaa tccagatata cgggtttgcc cagttgcccc acaacagggt
    12601 ggacacggta atggcggtca gaatcagcga accgcccatc gtcggcgtgc cgtttttgac
    12661 gaggtgggtt tgcggaccgt cggtacgcac tgcctgcccg catttgagcg cggtcagcct
    12721 gcgtatcgtc cacgggccga acatcaggga aaacgctaag gcggtcaacg ccgccatgac
    12781 ggcgcggaat gtggtgtatt gaaaaatatt cagaccggtt aaccagttgc tgaaatgtgc
    12841 gagccataaa aacatggggc ttcctttttt tgttttgtc
//
LOCUS       DAWFIE010000059        12655 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWFIE010000059 DAWFIE010000000
VERSION     DAWFIE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12655
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.059"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..2154
                     /gene="mfd"
                     /locus_tag="WGQ71_001505"
     CDS             <1..2154
                     /gene="mfd"
                     /locus_tag="WGQ71_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0707494.1"
                     /translation="GKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRD
                     LAEINIGDPVVHEEHGIGRYMGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISR
                     YSGQAHENIALHKLGSGAWNKAKRKAAEKARDTAAELLNLYARRAAQSGHKFEINELD
                     YQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVM
                     GGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALEGMADGTVD
                     IVIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTL
                     SMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDT
                     IENMRERLETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPN
                     ANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADEL
                     GAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLDAP
                     LGITTEIKLHSPALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQ
                     PVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPTEIILLIQNDKKYRLA
                     GADKLRFTAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            2303..2686
                     /locus_tag="WGQ71_001506"
     CDS             2303..2686
                     /locus_tag="WGQ71_001506"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ0707495.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            2708..3550
                     /gene="kdsA"
                     /locus_tag="WGQ71_001507"
     CDS             2708..3550
                     /gene="kdsA"
                     /locus_tag="WGQ71_001507"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ0707496.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCTHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPYQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEADNGKL
                     ILCERGSSFGYDNLVVDILGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            3565..4005
                     /locus_tag="WGQ71_001508"
     CDS             3565..4005
                     /locus_tag="WGQ71_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ0707497.1"
                     /translation="MMQTFRKISRYVATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAILTRLQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSMIFMATALLSAVLSWRLSGKDAV"
     gene            4053..5339
                     /gene="eno"
                     /locus_tag="WGQ71_001509"
     CDS             4053..5339
                     /gene="eno"
                     /locus_tag="WGQ71_001509"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ0707498.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYNLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            5354..5632
                     /gene="ftsB"
                     /locus_tag="WGQ71_001510"
     CDS             5354..5632
                     /gene="ftsB"
                     /locus_tag="WGQ71_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ0707499.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(5673..5963)
                     /locus_tag="WGQ71_001511"
     CDS             complement(5673..5963)
                     /locus_tag="WGQ71_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ0707500.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCYVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(6084..7217)
                     /gene="nrdB"
                     /locus_tag="WGQ71_001512"
     CDS             complement(6084..7217)
                     /gene="nrdB"
                     /locus_tag="WGQ71_001512"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ0707501.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGMHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(7278..8465)
                     /locus_tag="WGQ71_001513"
     CDS             complement(7278..8465)
                     /locus_tag="WGQ71_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ0707502.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(8458..9471)
                     /gene="dcm"
                     /locus_tag="WGQ71_001514"
     CDS             complement(8458..9471)
                     /gene="dcm"
                     /locus_tag="WGQ71_001514"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0707503.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            complement(9529..11808)
                     /gene="nrdA"
                     /locus_tag="WGQ71_001515"
     CDS             complement(9529..11808)
                     /gene="nrdA"
                     /locus_tag="WGQ71_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ0707504.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPCVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            12337..12638
                     /locus_tag="WGQ71_001516"
                     /pseudo
     CDS             12337..12638
                     /locus_tag="WGQ71_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
BASE COUNT         3336 a         3270 c         2859 g         3190 t
ORIGIN      
        1 ggcaaagcag ttgccgctca aagtgccatc gccgtcatca ccgaatccga tctctaccaa
       61 tacgtcgccc gttcgcgcgt ccacaaccgc cgcaagaaac acgccgccgt ttcagacggt
      121 ctgttgcgcg accttgccga aatcaacatc ggcgaccccg tcgttcacga agaacacggc
      181 atcgggcggt atatgggctt ggtaacgatg gacttgggcg gcgaaaccaa cgaaatgatg
      241 ttgctcgaat acgcaggaga agcgcagctt tatgtgcctg tttcacaact gcatttaatc
      301 agccgctact ccggtcaggc gcatgaaaac attgccctgc acaagctcgg cagtggcgcg
      361 tggaacaagg cgaagcgcaa agccgccgaa aaggcgcgcg ataccgccgc cgagttgctt
      421 aacctttacg cccgacgcgc tgcccaatcg ggacacaagt ttgaaatcaa cgagttggat
      481 tatcaggcgt ttgctgacgg cttcggctac gaggaaaccg aagaccaggc cgccgccatc
      541 gccgccgtga ttaaagattt gacgcaagcg aagccgatgg atcgccttgt gtgcggcgat
      601 gtcggcttcg gcaaaaccga agttgccctg cgcgccgcgt ttgtggcggt gatgggcggc
      661 aaacaggtcg ccgtactcgc cccgaccacg cttctggtcg agcagcacgc gcaaaacttt
      721 gccgaccgtt tcgccgattt ccctgtgaaa gtcgctagcc tttcgcgttt caacaacagc
      781 aaagccacta aagccgcgct ggaaggcatg gcggacggca cggtcgacat cgttatcggc
      841 acgcacaaac tggtgcagga cgacatcaaa ttcaaaaact taggtttagt gattatcgac
      901 gaagaacacc gcttcggtgt gcgccaaaaa gagcagctca aacgcctgcg tgccaatgtc
      961 gacatcctca ccatgaccgc cacgccgatt ccgcgtactt taagtatggc gttggaagga
     1021 ctgcgcgact tctcgctgat taccaccgcg cccagccgcc gcctcgccgt caaaaccttt
     1081 gtcaaaccct ttagcgaagg cagcgtgcgc gaagccgtgt tgcgcgaact caaacgcgga
     1141 ggacaggtat ttttcctgca caatgaagta gatacgattg aaaatatgcg cgagcggctg
     1201 gaaaccctgc tgcccgaagc ccgcatcggc gtggcgcacg gacaactgcg cgagcgcgag
     1261 ctggaacaag tcatgcgcga ctttttacag caacgattca acgtgttgct ctgttccacc
     1321 atcatcgaaa ccggtatcga cattcccaac gccaacacca tcatcatcaa ccgcgccgac
     1381 aaattcggac tggcgcaact gcaccagctt cgcgggcgcg tcggacgcag ccaccaccaa
     1441 gcctacgcct acctgctcac gcccgaatac atcaccaaag acgcagaaaa acgcctcgac
     1501 gccatcgcgg cagcagacga actcggcgca ggtttcaccc tcgccatgca ggatttggaa
     1561 atccgcggcg caggcgaaat cctcggcgaa gggcagtccg gcgaaatgat acaggtcggc
     1621 ttcacgctct acaccgaaat gctcaaacaa gccgtgcgcg atctcaaaaa aggccgccaa
     1681 cccgacctcg acgcgccgct gggcatcacc accgaaatca aactgcacag ccccgcccta
     1741 ctgcccgaaa gctactgccc cgacatccac gagcgtctcg tcctctacaa acgcctcgcc
     1801 gtctgcgaaa ccgtgcagca aatcaacgcc atacacgaag aactcgtcga ccgcttcggc
     1861 ctgcccgaac aacccgtcaa aacccttatc gaaagccacc acctgcggct tatggcaaaa
     1921 gaattgggta tcgatgccat tgatgcggcc ggcgaagcgg taacggtaac cttcggtaaa
     1981 aacaataatg tcgatccaac cgaaatcatc ctgctgattc agaacgacaa aaaataccgc
     2041 cttgccggcg ccgataagct gcggtttacc gcagagatgg aaaatatcga ggtcagaatc
     2101 aacaccgtaa aaaacgtttt aaaaaccttg caaaacagat gcctgcccaa ataaagccga
     2161 caccgcaatg ccgtctgaaa caccgttttc cttgtccgaa agccgccatt atgaatttga
     2221 aggaaactcc actataatac ggcattcaga tttccagacg gcatcgcgcc cgtcagaccg
     2281 cacacaaacc aaaaggaaat acatgttccg tactatgctt ggcggaaaaa tccaccgcgc
     2341 caccgtaacc gaagccgatt tgaactacgt cggcagcatt accgtcgatc aagatctgtt
     2401 agacgcggca ggcatctacc ccaacgaaaa agtcgccatc gtcaacaaca acaacggcga
     2461 acgttttgaa acctatacca tttcaggtaa acgcggcagc ggcgtgattt gtctgaacgg
     2521 tgctgcggca aggctggtgc agaaaggcga tatcgtcatc atcatgtctt acgtccaact
     2581 ctccgaaccc gaaatcgccg cgcatgaacc caaagtcgtc ttggtagacg gaaacaacaa
     2641 aatccgcgac atcatctcct acgagccgcc gcacaccgtg ctgtaaccct gaaaacggac
     2701 attgatcatg gatattaaaa ttaacggcat taccgtcggt aacgatgctc ctttcgtact
     2761 gttcggcggc atcaacgttt tagaagattt ggattccacc ctccaaacct gtacgcatta
     2821 cgtcgaagtt acccgaaaac tcggtattcc ctatatcttt aaagcatctt tcgacaaggc
     2881 aaaccgttcc tccatccatt cttatcgcgg cgtaggcttg gaagaaggct taaagatttt
     2941 tgaaaaagtc aaagcggaat tcggcatccc cgtcattacc gacgtacacg aaccctatca
     3001 gtgccaaccc gtcgccgaag tgtgcgatgt catccagctt cccgcattcc ttgcgcggca
     3061 gaccgattta gtggttgcca tggcaaaaac cggcaacgtc gtcaacatca aaaaacctca
     3121 gttcctcagc ccctctcaaa tgaaaaacat tgtggaaaaa ttccacgaag ccgacaacgg
     3181 gaaactgatt ttatgcgaac gcggcagcag cttcggctac gacaacctcg ttgtcgatat
     3241 actcggtttc ggcgtgatga aacagacttg cggcaacctg ccggttattt tcgacgttac
     3301 ccattccctg caaacccgcg atgccggttc tgccgcatcc ggcggtcgtc gcgcacaggc
     3361 tttggatttg gcacttgcag gcatggcaac ccgccttgcc ggtctgttcc tcgaatcgca
     3421 ccccgatccg aaactggcaa aatgcgacgg ccccagcgcg ctgccgctgc accttttaga
     3481 agatttttta atccgcatca aagcattgga cgatttaatc aaatcacaac cgattttaac
     3541 aatcgagtaa cacggtttcg ccttatgatg cagactttcc gaaaaatcag ccggtatgtc
     3601 gcaaccttgt ggctcggtat gcagattatg gcgggttata tcgccgcacc ggtgctgttc
     3661 aaaatgctgc ccaaaatgca ggcgggcgaa attgccggcg tattgttcga catcctctct
     3721 tggagcgggc ttgccgtttg gggcgcggtg ttggctgccg cctttgccat cctgacccga
     3781 ctgcaaaccg ccctgctgct ttttttattg tccgcccttg ccgccaacca atttttggtt
     3841 acccccgtta tcgaggcact gaaatacggg catgaaaatt ggctgttgtc gtttgtaggc
     3901 ggatccttcg gaatgtggca cggtatttca agcatgattt tcatggcaac cgccctactt
     3961 tcagcagttt taagttggcg gctttccggc aaagatgccg tctgaagccc tcccattttt
     4021 ttacctccct tcacttcact tggagaacat tcatgagcgc aatcgttgat attttcgccc
     4081 gcgaaatttt ggactcacgc ggcaacccca cagtcgagtg tgatgtattg ctcgaatccg
     4141 gcgtaatggg acgcgcagcc gtaccgagcg gcgcgtccac cggtcaaaaa gaggctttgg
     4201 aacttcgcga cggcgacaaa tcccgttatt cgggcaaggg cgtattgaag gcggtcgaac
     4261 acgtcaacaa ccaaatcgcc caagccctca ttggtatcga tgccaacgag caatcttata
     4321 tcgaccaaat catgatcgaa ttggacggta ctgaaaacaa aggcaatttg ggtgcgaatg
     4381 cgactttggc ggtttctatg gcggttgcac gcgccgctgc cgaagactca ggcctgccgc
     4441 tttaccgcta cttgggcggc gcaggcccga tgtccctgcc cgtaccgatg atgaacgtca
     4501 tcaacggcgg cgaacacgcc aacaacagcc tgaacatcca agagtttatg attatgcccg
     4561 tcggcgcaaa atctttccgc gaagcgttgc gctgcggtgc ggaaattttc cacgccttga
     4621 aaaaactgtg cgacagcaaa ggcttcccga ccacagtcgg cgacgaaggc ggtttcgccc
     4681 ccaacctgaa cagccacaaa gaagccctgc aactgatggt cgaggcgacc gaagccgccg
     4741 gctacaaagc gggcgaagac gtattattcg cattggactg cgcctccagc gagttctaca
     4801 aagacggcaa atacaacttg gaagccgaag gccgctccta caccaacgcg gaatttgccg
     4861 aatatctgga aagtctggtc aacgagttcc ccatcatctc catcgaagac ggcatggatg
     4921 aaaacgactg ggaaggctgg aaactgctga ccgaaaaact gggcggcaaa gtccaactcg
     4981 ttggcgacga cctcttcgtt accaacccga aaatccttgc cgaaggcatt gaaaaaggcg
     5041 tggcaaacgc actattggtc aaagtcaacc aaatcggtac tttgagtgaa accctgaaag
     5101 ccgtcgatct ggcaaaacgc aaccgctacg ccagcgtgat gagccaccgc tccggcgaaa
     5161 ccgaagacag caccattgcc gacttggcag tcgccaccaa ctgtatgcag atcaaaaccg
     5221 gttctttgag ccgttccgac cgcatggcga aatacaacca actgctgcgt atcgaggaag
     5281 aattggcgga agccgccgac taccccggca aagccgcatt ctaccaactg ggcaaataag
     5341 aaaaggttaa ggtatgaagt gggtaactgt cgttttatcc ttcgcacttg tctgttgcca
     5401 atacagcctc tggttcggca aaggcagcat cggacgcaac agcagtctga gagaacagat
     5461 tgccgttcaa gaagaaaaaa accagacact cgccctacgc aatcattccc ttgccgccga
     5521 agtctatgat ttggaaaacg gtcaagaagc catttcggaa atcgcccggg tagaactggg
     5581 ttatatccaa gacggtgaaa ccttttaccg actcatcagg cataaccggt aataccgtca
     5641 aaaagccgtc cgaaccaatg ttcggacggc ttttatttca acaaactgtc agacagcccc
     5701 tcatcctccc ccgacaaacc gcaatccagc ctgacatccc cctcgacgta acagcagcac
     5761 ggcagtatct cgtcccgccc caaaaaagcc aaaggcggct cccgataagt aacgcttccc
     5821 tccaaaatct tcacccggca cgatccgcaa tatccgcttc ggcactgata ttccaccata
     5881 tgccctgtcc gttccaagcc ttccaacagg gtctcgccct ccaaaagctc aaaaaaaccc
     5941 ttattcgtac caatgcgcgc catttccgac caatcaaaaa taaaaggaac aataaccgat
     6001 cctacccgct gtttttccca tcatacaaca tacaaatgcc gtctgaaaca tccggcttca
     6061 gacggcattt tttcaaaaaa catttacaac tcaaaatcgc ccaaatcatc cgtattcact
     6121 tcagaatcta tctgaccgat caaataagag gatatttcca cttcctgcgg cgcgacctgt
     6181 acgttgtcgg acgacagcca cgcattaatc cacggaatcg ggttttgatt tgcgccttca
     6241 aatccggccg gcaaccccac cgcctgcata cgcagattgg taatatattc gacgtattgg
     6301 gataagattt ctttgttcaa accgatcatc gaaccgtcct taaacaaata tgccgcccat
     6361 tctttttcct gttccgccgc ttttttgaag agttggaaac attcgtcctg caactcggcg
     6421 gcaatttccg ccatttcaga atcatcaaca ccagaacgca tcagattaag catatgctgc
     6481 gtgccggtca ggtgcagggc ttcgtcgcgg gcaatcagtt tgatgatttt ggcgttgcct
     6541 tccatcaact cgcgctcggc aaaagcaaac gagcaggcga atgaaacgta gaaacggatg
     6601 gcttccaaca cgttgacgca catcaggcag agatagagtt ttttcttcaa cccgcgcaaa
     6661 gatacggtaa cgggttttcc gccgacattg tgcatccctt cgcccaacag gttgtaatac
     6721 tgggtgtatt cgattaagtc atcgtaatag caggcaatgt cttcggcgcg ggcggtaatg
     6781 tattcgtttt cgacaatatc atcaaacacg accgacggat cattcacaat attgcggatg
     6841 atgtgggtat agctgcgcga gtgtatggtt tcgctgaagc tccacgtttc aatccacgtt
     6901 tccaactcgg gaatcgaaac caaaggcagc aaggcaacat tcggactgcg cccttggatg
     6961 gaatcgagca gtgtttggta tttcagattg ctgatgaaaa tatgtttttc gtgttcaggc
     7021 aggttggcgt agtcgatacg gtcgcgcgac acgtcgattt cttccggccg ccaaaaaaac
     7081 gacaattgtt tttcaatcag tttttcaaat acctcgtatt tctgctggtc ataacgggcg
     7141 acattcacag gctgaccaaa aaacatcggc tcattcagcg cgtcgttttt ggttttggga
     7201 aaggtgctgt atgacatagt taagtgttcg caggacataa tctattctct atattaatta
     7261 aaaatgatct gtaagtttta actaacctgc tgagacttta tatatttaat aaaattttct
     7321 aaagttaaaa catgggtttt cccaaaagtt ccaaatgttt ttacggattc tttagggaca
     7381 actaaggtta aattctcatc actcatttcc tgaatttgag tatcagatat accttcttgt
     7441 agagtaaata aatgcttatg aggaattcga tctgcttcat ttaaaacttg gcgccagcga
     7501 tccttacaag tagtctttga acctagcatt gtcagctttt cagtttgaaa tatgaaattc
     7561 cctgattcat ccatcgcatg gtactcttca cttccaggaa ataaaaaatc tggtttcttt
     7621 ttcccttctg tcttcgcttg cgtctcaaat tttaaggaaa attcgtcaaa tattcttgat
     7681 aaatgtaact ctaaagattt tcctgctctt gcttttctgc gatttgtaaa agaatgtgca
     7741 aaattaatga agctatccaa atcagtaatt tttgatttaa taatttcaaa ttcacgtttt
     7801 tcaaaaatgg aaaataattc atattccttt gaaacccatt gttttaacac attactaacg
     7861 ttagttttat ttaatgcaat attttccctt gccaaaactg ccatttcttc agttttgggg
     7921 aagttaggaa atagtgaaaa taattttgtt aggttatcct ctacttcttg ttttttaggt
     7981 aaatataaac ttcctggtaa aatgttagtt tctgcgataa atatttcaat atcttcatct
     8041 gaagataaaa caaaagcatg gaataataaa tctttataac aagctctgca caataccaat
     8101 aaggaaccac tatatttatc acttaaaaat tcaaaattct tgccaaaacc cgtaattctc
     8161 gcctcatttc tcgtgccttg gccgtaatat ttaaaattgc tattagtgac gcttccatct
     8221 tgccaattaa ttttgattga aatagtttta tttgttccct tttggcaaac tacatcaaag
     8281 aggtcgtctg aaaaatgttt ttgaatataa aatcctgatt ggtgactacc agtagtacca
     8341 acatcattgg gacgaatata acgacagtat actgcaattg atttatttgc tgtctgtatt
     8401 gctgaagcta ctaagtcatc ggatttattt actacttgta ttgtctgagc tgccaagtca
     8461 ttcatttttt aactcattta taatttgttt agcaatagct tgaaccaacg gtacagcaat
     8521 tgaattgcca aactgcttgt atgcagctgt cttggatact gcatcaataa caaaatcttt
     8581 aggaaatccc attaaacgcg agcactccct aggtgtcagc ttcctaggat tttttccttt
     8641 ctgagggatg agtatttcgg aaccatcttt gtaatatcgt gcagatagag ttcgtgatat
     8701 tccatctaaa tcaactaatc caaaaccaaa tccattaccc tttgccttat gttttttagc
     8761 gtaattttga aggtaaagcc ataagttatc agaaagagta aaagaattat ctacatcatc
     8821 ttccaaaatt tgctttaatt ttggttgtga ttctggtggg gaaggaaaat tgaaatttat
     8881 ttccttatta aaatattgtc tatcaaaacc tacaataaaa atacgctccc tattttgagg
     8941 aacataatat tttgcattca taacttgata aaatatctga tagtcaagct cttctaaagt
     9001 ccctttaatt actttaaatg tatttccttt gtcatgcgaa acaaggtttt tcacattctc
     9061 taaaagaaaa attttaggtc gatgttttcc aataatttca gcaacatcaa aaaatagagt
     9121 tccctgcgcc ttatctaaga agcctgtttc tcgtcctagg ctttttttct ttgaaacacc
     9181 agctatagag aatggctgac acgggaatcc tgctgttaat acatcaaact tacttggaat
     9241 agctgctttg gtttccttta atgtaatatc tccataagga atatcattaa aatttacttg
     9301 gtaggtttga cgggctttat catcccattc actagaaaat acacatcgcc caccaacatt
     9361 ctccattgca atgcgaaaac cacctattcc cgcaaataaa tcaataaaag taaatttctc
     9421 attatttaag gtaactatat tatctaattg attttgtatt ttatttttca tattttatat
     9481 ttcagatgat ttattaatct aaaggccatc tgaaaactcc ccctttcatc aaatcttaca
     9541 agccccgccc gcgcagccgt catcctgaat atcggtctgc gtatcgtccg caccgtcgcg
     9601 ggtgttatgg tagtacaggg ttttgacgcc gtatttgtag gcggtcagca ggtctttgag
     9661 catttgtttc atagaaactt tgccgccttc gaatttgccc gggtcgtagg cggtattggc
     9721 ggaaatcgat tgatcgacga atttttgcat cacgccgaca agtttcaggt agccttcgtt
     9781 gccgggaagc tgccacaggg tttcataggc atttttcagg gtttcaaact ccggcacgac
     9841 ttgtttcaaa atgccgtctt tcgatgcttt gaccgttacc aatccgcgcg gcggctcgat
     9901 gccgttggtg gcgttggcga tttgcgaact ggtttcagac ggcatgagcg cggtcagggt
     9961 ggagttacgc aggccgtatt tgacgatgtc ggcacgcagg ctttcccagt cgtaatgcaa
    10021 aggctcgccg cagacggcat ccaaatcttt tttgtaagtg tcgatgggca gtttgccttg
    10081 cgaataaacg gtttggttaa agagcgtgca cgcaccgtat tctttggcaa ggtttgccga
    10141 tgctttgagc aggtaatact gtatggcttc aaaggtacgg tgggtcagac cgagcgcgga
    10201 gccgtcgctg tagcggacac cgtttttcgc cagataataa gcatagttaa tcacgccgat
    10261 gccgagcgaa cggcgaccca tagtagaggt acgcgcggct tctaccggat atccctgata
    10321 atctaaaagt gcatcgagcg cacgcacggt caagtcggca agcccttcca attcgtccaa
    10381 gctgtttaat gcgcccaagt taaaggcaga cagtgtacac agggcgattt cgccgttcgg
    10441 atcgttgata ttgtccagcg gtttggtcgg cagggcgatt tccatacaca agttggactg
    10501 atgaacaggc gcgacgcacg gatcgaacgg gctgtgcgta ttgcagtgat cgacgttttg
    10561 aatgtagatg cgcccggtcc cggcacgctc ctgcatcagc gtggaaaaca ggtcggcagc
    10621 cggaatgata cgcttgcgta tgttcgggtc ttgctcgtat ttcgtataga gccgctcaaa
    10681 ttcgtcttgg tcggcaaaaa acgcttcgta caatcccgga acctcgttgg gcgaaaacag
    10741 cgtaatgttg ccgcccttaa tcaggcgggt gtacagcagg cggttgattt gcacgccgta
    10801 atcaagctga cggatacggt tgtcttccac gccccggttg tttttcaaca ccagcaggct
    10861 ttcggcttcg atatgccaca aggggtagaa caaggttgcc gcgccgccgc gcacgccacc
    10921 ttgcgaacag gatttgaccg ccgcctgaaa cattttgaaa aacgggatgc agccggtatg
    10981 ccgtgcttcg ccgccccgga tttcgctgtc cagtccgcgg atacgtccgg cattgatgcc
    11041 gatgcccgca cgctgggaaa cgtatttcac aatcgcgctg gtggtggcgt tgatggaatc
    11101 caggctgtcg tcgcattcga tcaatacgca gcttgagaac tggcgcgtag gcgtacgcac
    11161 gccgctcata atcggggtcg gcagcgatac tttaaatgtg gaaacggcat cgtaaaaccg
    11221 tttgacgtaa tcgaggcgtg tttctttggg atatttgctg aaaagacaca tcgccaccag
    11281 aacatataag aactgcggtg tttcataaat ctggcgggta acgcggttct gtaccagata
    11341 tttgccttcg agctgtttga cagcggcata ggaaaaggac atatcgcgtt cgtggtcgat
    11401 ataggcgttc agttcatcaa attcttcgcg gctgtaatcc tcaaggatat gcctgtcgta
    11461 ttttccggca tcggtgagct ttttaacgtg gtcgtaaagg tgcggcggct cgtattcgcc
    11521 gtaggctatt ttacgcagat ggaaaatcgc caaacgcgcg gcaaggtatt ggtagtccgg
    11581 agtatcttcc gaaattaaat cggcagcggc cttgatgatg gtttcgtgga tgtcgtcggt
    11641 gcggatgccg ttgtagaact ggatgtgcga tttcaactcg acctgcgaca cggaaacatt
    11701 ttccaatcct tccgccgccc aagtaacgac acggtgaatc ttatccaaat caatggcttc
    11761 taatcttccg tctcgtttgg ttactttcaa atcagtcggt gtattcatcg cttcctcttc
    11821 cactcttgat attcaagaca cggtcttttc aaataaatta agacagacaa tatagtggat
    11881 ttttggcatt ttctccagtc ttgacaacgg ttgcattttt cagtttcagg caatgcgcga
    11941 taaaacccgc ctgtcgtatt tttcctataa tatttgtttt atctgataat tctttaccga
    12001 taaaaaacgg gtaaattttt tgccttttga ccggctccgg ctacaaggcg gtgaaataag
    12061 gattttccga cgaaaaagga aagcttccgg ttttctgccc ctgcgaattg ttaaattttg
    12121 caaagtatga ttttgccgcg ccgccgccga caaattccat tttcttaccg attgaaattt
    12181 attattgaga ttaatgtgtt atttgaatcc gcatatcaaa cggcaagttt tgtcggctga
    12241 atgagtctga aactgccgac aatttgcccg ttccatcctt ccatcctata ctggaaggaa
    12301 tgacaaactg aaaggatcgt catatctgtc aaaaagatgc ttgccatact gttgtctgca
    12361 atattgggac tggtatcgac aactgccgct gccggtacgt cagaacccgc ccaccgccat
    12421 accaaacata tccgcaaggc aaactgcacc ccgaatgcag gaaatatttg gaacgccgtg
    12481 ccgcgtggta ccgatcgcaa ggcaacgtgc aggaattgcg cgaaaacaaa aaggcgcgca
    12541 aagcattccg ctccctgcct tataaggaac agaaaaccca atgccgggcg gcttatgagg
    12601 ctttcgatga tttcgacggc ggcaggttcc gccgttaatc ccatataaaa tatgc
//
LOCUS       DAWFIE010000060        12275 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWFIE010000060 DAWFIE010000000
VERSION     DAWFIE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12275
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.060"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            202..1878
                     /gene="ettA"
                     /locus_tag="WGQ71_001517"
     CDS             202..1878
                     /gene="ettA"
                     /locus_tag="WGQ71_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ0707505.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIAGNLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
     gene            complement(1938..2423)
                     /locus_tag="WGQ71_001518"
     CDS             complement(1938..2423)
                     /locus_tag="WGQ71_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ0707506.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(2416..3372)
                     /gene="thiL"
                     /locus_tag="WGQ71_001519"
     CDS             complement(2416..3372)
                     /gene="thiL"
                     /locus_tag="WGQ71_001519"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ0707507.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            complement(4229..4957)
                     /gene="rmpM"
                     /locus_tag="WGQ71_001520"
     CDS             complement(4229..4957)
                     /gene="rmpM"
                     /locus_tag="WGQ71_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ0707508.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            5321..6271
                     /locus_tag="WGQ71_001521"
     CDS             5321..6271
                     /locus_tag="WGQ71_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ0707509.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(6385..7119)
                     /gene="fnr"
                     /locus_tag="WGQ71_001522"
     CDS             complement(6385..7119)
                     /gene="fnr"
                     /locus_tag="WGQ71_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ0707510.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            7322..8743
                     /gene="hemN"
                     /locus_tag="WGQ71_001523"
     CDS             7322..8743
                     /gene="hemN"
                     /locus_tag="WGQ71_001523"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ0707511.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(9096..10670)
                     /locus_tag="WGQ71_001524"
     CDS             complement(9096..10670)
                     /locus_tag="WGQ71_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ0707512.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            10995..12095
                     /locus_tag="WGQ71_001525"
     CDS             10995..12095
                     /locus_tag="WGQ71_001525"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ0707513.1"
                     /translation="METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRVVLNIGGIANISVLPSDAPAFGFDTGPGNMLMDAWMQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLGGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADARQMYICGGGIRNPVLMADLAEYFGTRVSLHSTAELKLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            12214..>12275
                     /locus_tag="WGQ71_001526"
     CDS             12214..>12275
                     /locus_tag="WGQ71_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0707514.1"
                     /translation="MKILLLLIPLVLTACGTLTG"
BASE COUNT         3183 a         3435 c         2986 g         2671 t
ORIGIN      
        1 tgctttggaa aatccgaaat ggttgccggg gcggcgatcc cctatcatta ttattttttt
       61 gtctatataa tttcaaaggg ataagcggat tttatgaatc ctgcccgatt ttggcaatac
      121 cggttcgcgg ataaactggc ttaaatcaaa ttatcggtta aaatggccgt ctgaaatttg
      181 tttgatgaaa acgagaaaac catgtcccaa caatacgtct attctatgct gcgcgtgagc
      241 aaggttgtgc cgccgcagaa aaccatcatt aaagatattt ccctttcttt cttccccggc
      301 gcgaaaatcg gcctgctcgg tttgaacggc gcgggcaagt ccaccgtgct gcggattatg
      361 gcgggcgtgg ataaggaatt tgagggcgaa gccgtgccga tgggcggcat caaaatcggc
      421 tacctgccgc aagagcctga gcttgatccg gaaaaaaccg tgcgcgagga agtggaaagc
      481 ggtttgggcg aagtggctgc cgcgcagaaa cgtttggagg aagtgtatgc cgagtacgcc
      541 aatcctgatg cggattttga cgcgttggca gaagagcagg gccgcttgga agcgattatt
      601 gcggcaggtt cgtccacggg cggcggtgcg gaacacgaat tggaaatcgc cgccgacgcg
      661 ctgcgcctgc cggaatggga tgccaaaatc gataatttgt ccggcggtga aaaacgccgc
      721 gttgccttgt gcaaactctt gttgagcaag cccgatatgc ttttgctgga cgagccgacc
      781 aaccacttgg atgcggaatc ggtcgaatgg ctggagcaat ttctcgtgcg cttccccggc
      841 acagtcgttg cggtaacgca cgaccgctac ttcctcgaca atgccgccga atggattttg
      901 gaactcgacc gcggccatgg tattccgtgg aaaggcaatt actcgtcttg gctggagcag
      961 aaagaaaaac gcttggaaaa cgaggcaaaa tccgaagccg cgcgcgtgaa ggcgatgaag
     1021 caggaattgg aatgggtgcg ccaaaatgcc aaaggccgcc aagccaagtc caaagcgcgt
     1081 ttggctcgtt ttgaagaaat gagcaactac gaataccaaa aacgcaatga aacgcaggaa
     1141 atcttcattc ccgtcgccga gcgtttgggt aacgaagtga ttgaatttgt gaatgtttcc
     1201 aaatcgttcg gcgacaaagt gctgattgac gatttgagct tcaaagtgcc tgcgggcgcg
     1261 attgtcggca tcatcggtcc gaacggcgcg ggtaaatcga cactgtttaa aatgattgcg
     1321 ggcaaagagc agcccgattc cggtgaagtg aaaatcgggc aaaccgtgaa aatgagcttg
     1381 attgaccaaa gccgcgaagg tttgcaaaac gacaaaaccg tgttcgacaa cattgccgaa
     1441 ggtcgcgata ttttacaggt tggtcagttt gaaattcccg cccgccaata tttgggacgc
     1501 ttcaatttca aaggcagcga ccaaagcaaa atcgccggca acctttcagg tggcgaacgc
     1561 ggtcgtctgc acttggcgaa aaccttgttg ggcggcggca atgtattgct gttggatgaa
     1621 ccgtctaatg accttgacgt agaaaccctg cgcgcgctgg aagacgcatt gctggaattt
     1681 gccggtagcg tgatggtgat ttcgcacgac cgttggttcc tcgaccgtat tgctacgcat
     1741 atcttggctt gcgaaggcga ctccaaatgg gtgttctttg acggcaacta tcaggaatac
     1801 gaagccgaca agaaacgccg actcggcgaa gaaggcacga aaccgaaacg catcaaatac
     1861 aaaccggtaa cgcgttaacc tccgaaacaa tgccgtctga aaggctttca gacggcattt
     1921 ttacaaggca gcaccgttta aaacagcatt gcaatcctca agacaatcaa agtcatcacc
     1981 gcagccgcca tatcgtccgc cataatgccc aaaccgccgt gcagattctt gtcaaaccaa
     2041 ccgacgggag acggtttgag cgcgtcaaac agacggaata ggacaaatgc cgccagccac
     2101 cacgtccacc tgaacggcac aaacgccagc acaaacagca tggcgacaat ctcgtcccaa
     2161 acaatcccac catggtcgct gacacccgtt tcacgttccg cataagcgca aatgcgtatg
     2221 ccccacataa acagcacgat acacaaaaaa gccagcagca gcccgtctat gccgagcaaa
     2281 atcagcacaa atgccaaagg cagtgccgcc aaagtgccaa atgtacccgg cgcgaacgga
     2341 gccagcccgc tgccgaaacc aaaagccaaa aaacacaacg gtcgtttcaa cagccacgca
     2401 aagtcaggtt taaaatcagc caaaatgatc gaatcctaaa gaatgtagtt ccaattccct
     2461 gccgtcagca tctaaaacct tcagacggca tcctccgttg attttgccga tgcgcgttac
     2521 cggcacgccg caccgttctg ccgcatcaag cacgcggctg cggcaacttt ccggcgcggt
     2581 aaacaccagc tcgtaatcgt cgccgcccgc caaagtataa gacagccatt gcgctcgggg
     2641 caaaatatct ttcaatacgg ataaagacgg cagcgaatcg gcccaaattt ccgcccccac
     2701 gccggaagcg gtcaggatat gccccaaatc ttgcgcgagg ccgtctgaaa catcctgcgc
     2761 cgcccgggca aacggcaaca gcgcaagccc cagcccaacc cttggctcgg ggcggagcag
     2821 cttctgttcg cattcggcaa acacatcatc cggcaacaca caccgtttca gacggcagtt
     2881 caaagccgcc gccgccatac cgacacgccc cgacacccaa atatcgtcgc ccgcaaccgc
     2941 cgcatcacgc cgcaatgccc tacccttcgg caattcgccg ataatggtta cattgaacgc
     3001 catatcgccc ttggtcgtat cgccgccgat taacgttacg ccaaactttt ttgccaaacc
     3061 gaaaaagctg ccgcaaaacc gtttcagcca tacctcatcc aattcgggca aagccgcgct
     3121 cagcaacacc caacgcggta tcgcacccat cgccgccata tctgaaatat tgacggccaa
     3181 aaccttccaa gccaagtctt caggtttgac atctgcaaaa aaatgcctgt ccttcaaaag
     3241 catatccgca ctgaaacaca aatcgaagcc ttcacgcggg cggacaatcg ccgcatcgtc
     3301 gcctatgccc aatacgacat cattatccgt gcctgtttgc aaataccgtt tgatgaagtc
     3361 aaattctttc atatcccaaa atatccggcg ggcgaatcca atttaaatca tagcaaggca
     3421 gcaaaggccg tacaaacaat aaagcggctc attcagtgtt tcagcgtatt tcacttttag
     3481 ctttggccat accgtacccg tatgcaccga ccaccctgtt tcaacacaca aacggcatat
     3541 caaagcaccg cactgcacgc cgccccgaat gcaaagcggc aacttccgcc accgattagg
     3601 ctcaatcgct gcaactgacg gggtttcaac cgaaaagggc tgatgataaa accatatccg
     3661 gcatacagtg ccgcacacat ctggcacgat tccatccatt tcccaatgaa ggggaaaggc
     3721 gtgaatttgt atgaattatc cctttcaggg cttgatacag aaaggattcc ctttctatgg
     3781 gcaaacccac atcctaccgc cttaaaatca gagtttcaac cgatatagtg gattaaattt
     3841 aaaccagtac agcgttgcct taccttagct caaagagaac gattctctaa ggtgctgaag
     3901 caccaagtga atcggttccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
     3961 tttttgttaa tccactataa agcacggcaa tgggacacaa acctgaatct gaatctgaat
     4021 ctgaatctga atctgaatct gaatctgaat ccgaacctgc accccgattc tgacgatagc
     4081 cgtttcagaa aaccacccaa aaaaacaaac cccgcaaaag cgggggtttg tttcacatca
     4141 tcgatgccga ccgacgtttc acgaatattg caacagtacc cggtacaaaa tccgctaacc
     4201 tcatgcatcg gcaagatatt gcctagcctt agtgttggtg atgattgtgt gccggcacaa
     4261 cctgacgggt tacgatgctg cggattttca catccacacg gcggtcaggt tcgatacatg
     4321 caatcagagc ctcacgtttt ttggctttag agactttcgc acccagtttg gcaacttcgg
     4381 cttcacaaac ttgagtcatt tgcgcttgag attcgcccaa gccgacagca gaaattctag
     4441 aaacaggtac gccgttgctg accaggttgt ttgccactac gtatgcgcgg cgttcggaca
     4501 gggcctgatt gtatttgtca gaacccataa agtcggtatg gccttcgacg cggacagatt
     4561 ggatattggt ttgacccagg cgttgcgcca atactttcag gttgtcttga gcttcggcgc
     4621 gcaatgaatc cttatcgaaa ccgaacaggg ttttggcaga cagggaaatg gtttcatcaa
     4681 catattgcgg agcctgctcc acaacgacga caggcgcggg tgcgggttcg ggttctggct
     4741 cgggttcggg ggcagcaacc gcatcgccgc attctacgcg accttggctt gctttatcaa
     4801 agtaggcgtt tttccagcat tcgccatagt tgttgcgtac gatttcgttc gactggccgc
     4861 ttacggtgta accctgaacg gacgcctcgc ccgcaacagc agtgccggaa gcgagcaatg
     4921 caacgaataa tgcgcttaat ttcagctgtt tggtcatttt attccctcat taaatttgta
     4981 cagcagatac agaagcaact gctgaattgc caacatacag gccgctgtag aaacatggcg
     5041 gcaaatcgga tatttcagtc tccactataa agaagcaggg ggaggactgt caatactcaa
     5101 cgcctgaaaa caaccgtaaa acagcctgtt tcctgatagt ctatcatgcc gaaaaatcaa
     5161 ttccgtgtca ttactttagg gggatttttt ccatatcgca cgattgccgt tgcacaaaaa
     5221 caacaaacac aacggcaaag ccccataccg taccggcaag aaaatatgat aaattataaa
     5281 caatgttcag ccacccgaca cagacccaca ccgacccgcc atgaaattac aacaattgaa
     5341 atacgcctta gaagtttacc agcacaacct gaacgtttcc gaagcggccg aagccttgtt
     5401 tacttcgcaa cccggcatct ccaaacaaat caaattgctg gaagaagaaa tcggcattca
     5461 gatttttatc cgcagcggca agcgcgtggt ttcggtctcg cagccgggca aggtggtttt
     5521 ggatattgcg gaacgtattt tacgcgatgt acagaacatt aaaaatatcg gcagcgagtt
     5581 taccggacag gacagcggtt cgctgacggt tgccacgacg catactcagg cacgctatgc
     5641 cctacccttg attgttgccg attttgtgaa acgctatccg aaagtcaacc tgaccatcaa
     5701 acaggggagc cctgccgcca tcgcacgtat ggttacctca ggcgaggcgg acttggcgat
     5761 tgttacggaa cggatagacg accatcccga actgggaaaa cttccctgct atgactggac
     5821 tcatgcggtt atcgtaccga acgaccaccc cttgctcgaa tgcagaaacc ccctccgcat
     5881 tgaagatttg gcgaggtttc cgctgattac ttatgaattt gcattcaatg cgggcagcag
     5941 catcgcgcgg gcattttcca aagcccgttt ggaacagccg gatgtcgcat tggcggcggc
     6001 ggatacggac gtattgaaga cttatgtgcg cttgggtttg ggcgtgggac tgatggcgaa
     6061 aatggcgtac aacccggata cggacggcga tttgcagctt gtggatgcgg cacacctgtt
     6121 cgagccgtcg ccgacgtgga ttgctttgcg cagcgatact tatttgcgcg gatatgccta
     6181 cgactttatc caagcgtttg cgccgcacct gacacgcgag aaggtggata ggattctgta
     6241 cacgcccatc agcgaggatt tttcgattta ggcggctgcc ggttttcaga cggcactttg
     6301 cggcagatac aacaaacagg acagatgttt ttcgtctgcc ctgtgtttat tgagaatgcc
     6361 gtctgaaatg ttcgtacggg ctaatcaaat ggcgtgcgaa caaccggata ccatcttctt
     6421 caacacctgc agattgagga tcttgatgtg tttgtgttcg acggaaatca atccttcctg
     6481 atgaaattta gataatgtgc ggctgacggt ttcaagtttc agcccgagat aactgccgat
     6541 ttcttcgcgg gacattctta agatgaagtc gttggcagca aaacctcggg aataaaggcg
     6601 ttgggaaagg ttcaacagaa aggcggcaat ccgctcttcg gcgcgcatat tgcccaacag
     6661 cagcataaca ccttggtcgc gcacgatttc acgactcatc atgcggaaaa agtgggtacg
     6721 cagactgggg atattctgcc ccagttcttc gatgtgggta aacggcagtt cgcacacttc
     6781 gctgtcttcc aaggcgaccg cgtcgcaact gtgcacatgg gaacagatgc cgtccatgcc
     6841 gatgagttcg cccgacataa agaaacccgt tacctgatcg cggccgtcct gactggcgac
     6901 ggttgttttg aagaagcccg aacggatggc aaagagcgag gtaaaggctt cgccgacaca
     6961 gaacaggtat tcgccctttt tcaggcggcg gctttgacgg atgacggcat cgagttggct
     7021 gagctcgttg ggcagcagcc cgacaggcag gcagagttcc cgcaaagaac aggaagaaca
     7081 cagcgttttc atctgatgtg tagtattatg cgaagccata ccgtaccttt ttgtgcgctt
     7141 tgccccatca tgattatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgtc
     7201 cttatttaaa tttaatccac tatatgtgct tattgacaca tatcaagaca ggtttatcat
     7261 actgtggcat tctaccaaac tatccaaaat ttgactaaca tcataaaacc gcacacacac
     7321 catgaaaatc attcagatac agaacaatca caatgtaaac gatgaccgcc ccgagtttga
     7381 ccgcgcgctg attgccagcc tgcccgccag cggcccgcgc tacacttcct accctaccgc
     7441 cgaccgtttc catgacggtt tccgcgaagg cgaatatatc aaagctttac atttgcgcgg
     7501 tatgggcgcg ttaaacaaac cgctttccct ttacattcac attccgttct gcaacaccat
     7561 ctgctactac tgcggctgca acaaaatcat caccaaagac aaaagccgcg ccgatgccta
     7621 catcgaatat cttgaaaaag aaatggaact gctcgctcca catctgaacg gacggcacca
     7681 gcttgcccaa ctgcacttcg gcggcggcac gccgaccttt ttgagcgacg aacagatcga
     7741 acgtgtcttc cgcatgatac gcaaacattt cgagttaatc cccaccggcg aatactccat
     7801 cgaaatcgac ccgcgcaaag tcagccgcga caccgtcctc atgctcggca gactcggctt
     7861 caaccgcatg agcatcggca ttcaggattt cgaccccaaa gtgcaggcgg cggtcaaccg
     7921 catccaaagt tacgaagaaa ccaaagaagt catcgatgcg gcgcgcgaag cggggttcaa
     7981 atccgtcagc gtcgatttga tttacggcct gccgcaccag acttcggaaa gcatcaaaac
     8041 caccatcgat accgttttgt cgctcgatcc cgaccgcctc gccctttatc actacgccca
     8101 cctgccgcac gtgttcaaac cgcaacgccg catcgatacc gccgccgttc ccgacagcga
     8161 agagaagctc gatatgctgc aatactgcgt ccaaacccta accgaacgcg gctacgtctt
     8221 catcggcatg gatcatttcg ccaaacctga cgacgaactc tccatcgccc tcaaagaagg
     8281 cttcctccag cgcaacttcc aaggctattc gacctacgcg gattgcgatt tggtcgccat
     8341 cggcgtgtcg tccatcggca aaatcggcag cacctattcc caaaacgaac gcgacatcga
     8401 tgcctactat gccgccatcg acgaaggcag actgcccatc atgcgcggtt atcagctcaa
     8461 tcaggacgac atcctgcgcc gcaacatcat tcaggatttg atgtgccgtt tcgcgctcga
     8521 ctaccggatt tacgaaagta tgttcggcat cccgttcgac cgctacttca aagacgaact
     8581 ggcggatttg gaaaaactcg ccggtttggg attggtgcgc ctgaacagcc acgggctgac
     8641 cgttacccca aaaggacgct tcctcatccg caacatcgcc atggtcttcg attaccacct
     8701 gcgccataaa gaaaccaagg cgaaatattc gcaaacggtg tgattgcgtc taacgtacaa
     8761 atgccgtctg aaaggctttt tcagacggca ttttgctgcc ggcaggataa gtgttttcaa
     8821 gaacaggcgg cggcatatca taacgttccg cacctttgtg tccgaccgtt ccgaaaccaa
     8881 gatatagtgg attaacaaaa accagtacag cgttgcctcg ccttgctgta ctatctgtac
     8941 tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc actataccaa acggcatatc
     9001 ccgacagaac agattgtgca taaggcacaa gcccgcacat tccatcaaca aaatgccgtc
     9061 tgaacacggg ttcagacggc atcagtattt tacaatcaga atactgcctg taaaaccaag
     9121 tagcagacaa ccgacaatac ggcggcggca ggcagggtaa tgacccacgc caaaccgatg
     9181 ggcttcatca gtttccagtt ggcattgcgg ttgaccagac cgatgccgag taccgcgccg
     9241 accaagatat gcgtactgga cacgggcagc cccatcagcg acgcgcccat cacgacggag
     9301 gcggcggaca gttcggcggt aaaacccgag gcggggtgca tttcggccaa actcgtaccg
     9361 acggttttaa tcacctcttt accgacaaac cacaaaccga caatcagcgc gatgccgaaa
     9421 gtcagcatcg caatcggggg gacgacattt tgcgcggcaa cgctgttggt acgcaaaaca
     9481 tccataatcg cggcaaacgg accgatggcg ttggcgatat cgttcgcacc gtggctgaat
     9541 gcgaagccgc aggcggtaaa gacctgcatc catgaaaaca tctgaaaggt cgatttgccc
     9601 aagtctttac gcttgaggct tttggcaaac acaaacgtcc ccatccacac cgccgcgcct
     9661 atcataaaga tggtcaggaa gctgttgacg ttgctcatcc ccaaatgcag gtttttcaag
     9721 cccttgaaaa tcagcatagc ggaaatcatc atcgcgccga acgaagcgat aaagggaatc
     9781 caagaatgca gcgctttgta ggaatcgaca ttgtttttac ggttgtcgaa cgcataaaga
     9841 ccgcggtaat actccgattg cagctcttgc ggatcgaatt cgggttcgtc gtaaatttgc
     9901 gcgtcgtgcg ccattttggt ggcgtactcg actttttcgg cttcggacaa accctcgaaa
     9961 aacaggcggt gccgttcttt ataggccttt ttttcctgct tgatgccctt gagcgtgcct
    10021 tccgcccaag cgttgtaatc taagacgttt ttcttgacgc gcgaaaacag gaaataggac
    10081 accgcgccgc ccaacacggg cgacaatacc caagaaacac caataccgcc cagcttgccc
    10141 caacgtatca aatcgcccga tgcggcatcg ttcattaccg ccatacatac cgcgctgccg
    10201 acaatgccgc cgataatgga atgggtggta gataccggaa gccctttttt cgaagcaaac
    10261 aacagccaca acgccgccgc caaaagcgcg gacatcataa taaacacaaa ctgtatgggt
    10321 tcgaaatcaa cacccttcaa atcgacgatg cctttgcgta tggtattggt tacctcgccg
    10381 cccgcgatga ccgcgccgct gacctcaaat accgccgcaa tcagcaaagc ctgcgggatg
    10441 gtcagcgtac ccgcaccgac gctggtgccg aaagaattgg caacatcgtt gccgccgatg
    10501 ttgaacgcca taaacacgcc gaacatcgtg gcaataatga ataaggcggc gtaattgtaa
    10561 tgggtataac ccaagcccca ataaataaaa tagccgactg tacctaccag catggcggca
    10621 aagacggcgt tgatggtttt aacatttgcg ctcatttgga tttgagccat ggcaatcttc
    10681 ctttaatgtg gtggaaatca tgagtgttgc cgacactgta ctgctgcctc cggtcgaact
    10741 gccgtttcgg gatatggcag acgcatcctt ccttctgaac cgcatccgaa tacccccaaa
    10801 cctgaccgaa tgcaaaactg ctgccggaaa cggtttgtat gacacttcta ctactggatg
    10861 ttgcgggcgc attataatat ttttccatcc gtcttgaaac atttatttac actttattta
    10921 cactgcggcg gcaaatcggt atacgagcgt caatacacgt taaaatggcg ttttgcacca
    10981 gtttgggagt gatgatggaa acacagcttt acatcggcat catgtcggga accagcatgg
    11041 acggggcgga tgccgtactg atacggatgg acggcggcaa atggctgggc gcggaagggc
    11101 acgcctttac cccctatccc gacaggctgc gccgccaatt gctggatttg caggacacag
    11161 gcgcagacga actgcaccgc agcaggattt tgtcgcaaga actcagccgc ctgtacgcgc
    11221 aaaccgccgc cgaactgctg tgcagtcaaa acctcgcgcc gtccgacatt accgccctcg
    11281 gctgccacgg gcaaaccgtc cgacacgcgc cggaacacgg ttacagcata cagcttgccg
    11341 atttgccgct gctggcggaa cggactcaga tttttaccgt cggcgacttc cgcagccgcg
    11401 accttgcggc cggcggacaa ggtgcgccgc tcgtccccgc ctttcacaaa gccctgttcc
    11461 gcgatgacaa agaaacacgc gtggtactga acatcggcgg gattgccaac atcagcgtac
    11521 tcccctccga cgcacccgcc ttcggcttcg acacagggcc gggcaatatg ctgatggacg
    11581 cgtggatgca ggcacactgg cagcttcctt acgacaaaaa cggtgcaaag gcggcacaag
    11641 gcaacatatt gccgcaactg ctcgacaggc tgctcgccca cccgtatttc gcacaacccc
    11701 accctaaaag cacggggcgc gaactgtttg ccctaaattg gctcgaaacc taccttggcg
    11761 gcggcgaaaa ccgatacgac gtattgcgga cgctttcccg ttttaccgcg caaaccgttt
    11821 tcgacgccgt ctcacacgca gcggcagatg cccgtcaaat gtacatttgc ggcggcggca
    11881 tccgcaatcc tgttttaatg gcggatttgg cggaatattt cggcacacgc gtttccctgc
    11941 acagcaccgc cgaactgaag ctcgatccgc aatgggtgga agcagccgca tttgcgtggt
    12001 tggcggcgtg ttggattaat cgcatccccg gtagtccgca caaagcaacc ggcgcatcca
    12061 aaccgtgtat tctgggcgcg ggatattatt attgagcgca aataccgtct gaaagccatt
    12121 tccgccgctc agacggcatt ttcgcccctt tcgtttacaa acccttaaaa tccatttaca
    12181 ctcaaacccg ttcaaaacaa aacaaaccct gccatgaaaa tcctgctcct cctcatcccc
    12241 ctcgtcctaa ccgcctgcgg cacactgacc ggcat
//
LOCUS       DAWFIE010000061        12126 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWFIE010000061 DAWFIE010000000
VERSION     DAWFIE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12126
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.061"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(405..1466)
                     /locus_tag="WGQ71_001527"
     CDS             complement(405..1466)
                     /locus_tag="WGQ71_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0707515.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKVAKVAKV
                     AKVAKVAKVAKVAATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKA
                     NLPKDAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNPALKNLSP
                     AQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQH
                     ILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVM
                     VPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAE
                     QATVNLLRDLHSGAYVDIR"
     gene            complement(1571..3979)
                     /locus_tag="WGQ71_001528"
     CDS             complement(1571..3979)
                     /locus_tag="WGQ71_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ0707516.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVQGSGEASIPEDYTRIVADRMEGQSQ
                     VQVRAEGNVVVERNRTTLNADWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKNDAVMLDGSVGKKPRSRS
                     DWVAFASSGIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            4049..5053
                     /locus_tag="WGQ71_001529"
     CDS             4049..5053
                     /locus_tag="WGQ71_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ0707517.1"
                     /translation="MQRQIKLKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARADGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(5110..5808)
                     /locus_tag="WGQ71_001530"
     CDS             complement(5110..5808)
                     /locus_tag="WGQ71_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0707518.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRIEH
                     VVWQKEMLPLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQNPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(6088..6195)
                     /locus_tag="WGQ71_001531"
                     /pseudo
     CDS             complement(6088..6195)
                     /locus_tag="WGQ71_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            6253..7791
                     /gene="murJ"
                     /locus_tag="WGQ71_001532"
     CDS             6253..7791
                     /gene="murJ"
                     /locus_tag="WGQ71_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ0707519.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFDWAHAGGMQKAARLFILIVIGGCLYFASLAALGFRPRHFK
                     RVES"
     gene            7924..8880
                     /locus_tag="WGQ71_001533"
     CDS             7924..8880
                     /locus_tag="WGQ71_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ0707520.1"
                     /translation="MILTPPDTPLFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRDRGWHGVVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTFAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGNRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPTPDEVSETV
                     TLFQPAHGGHVGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            8930..9712
                     /gene="trpC"
                     /locus_tag="WGQ71_001534"
     CDS             8930..9712
                     /gene="trpC"
                     /locus_tag="WGQ71_001534"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ0707521.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDL
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRTRGVHTFLIGETFMRAADIEAEVGKLF"
     gene            9777..12077
                     /gene="recQ"
                     /locus_tag="WGQ71_001535"
     CDS             9777..12077
                     /gene="recQ"
                     /locus_tag="WGQ71_001535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ0707522.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGKQESIVNALAGGESLMVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSSTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGI
                     LAERYPNVPRIALTATADAATRADIKHYLHLDNAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEVIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKIDRFGHGILEVCRNTADFSHNAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         2459 a         3378 c         3468 g         2821 t
ORIGIN      
        1 ttcgcgggaa tgacggcata gaggtttctg ttttcccgat aaattcctgt tacttttcgt
       61 tgctagattc ccgcctacgc gggaatgacg atggaaagat tgttgttgct tcggataaat
      121 ttctgcagtt ttaaataacc ggattcccgc ctgctcggga atgacggcat aggttttttt
      181 gtttttccga taaattacca caacccaaaa tcccgtcatt cccgcgaaag cgggaatcca
      241 gacctttaaa ttccagccat tcccgataaa ttcctgttac ttttcgtttc tagattcccg
      301 ctttcgcggg aatgatgaat gacggtgtaa aagtaactcg aaatccaaaa agccataccg
      361 cccggatttt tgccgatcgg tatggctttt tgcttcaaac cgccttagcg gatgtcgaca
      421 tacgcgccgg aatgcaggtc acgcaacagg ttgacggttg cctgttcggc tttttgttgg
      481 aagatgtatt gccgcacgga attgcggata cgttcctgag gtgtgccggc atcgcgcact
      541 tcgttcaatt tgatgatatg ccagccgaat tgggtgcgga cgggcgcgcc gacctgtccg
      601 ggtttgagcg cgtggacggc ttcttcaaag gcgggaacca tcacgccgtc ggcaaaccag
      661 cccaaatctc cgccgttgcc cgcgctcgcg tcttgcgaat attggcgcgc caggctggaa
      721 aagtctgtgc cgctgcgggc ctctccgtag attttgcgga tggtgctttc cgcgccgacg
      781 gcagcgtttt cgctgtcggc tttaatcagg atgtgttggg cgcggtattg gcgcaacggt
      841 gcgccttcgg gcagggtgat gccttgtttt tgcgcctgct cgaggaaggc atcgatttca
      901 gcttcgctca ctcggctgtt ctgcatcact gcctgctggc ggactttttc ggcaatgatg
      961 ttgtcggcaa aatcgcggcg ttgggcgggg ctgaggtttt tgagggcggg attttttgcg
     1021 acgacggcat cgatttccgc ttcgcttgct tgaatgttgc ggcgtttgcc cgcctgtaca
     1081 atcagggatt ggttgacaag ctgcatcagc acctgtcggg acagctcgga ttcgcttatc
     1141 tgcgcgtctt tgggcaggtt ggctttggct tcggcaacgg cttcggcaag ccggcggcgc
     1201 gtgatgactt cgttgtcggc aacggcggca atgccgtctg aaaagcggat accgccctgc
     1261 tgttgtgcgg gtgcggcttc tttttgcgcc gtggcggcaa ctttggcaac tttggcaact
     1321 ttggcaactt tggcaacttt ggcaactttg gcaactttgg cagctttggc agctttggcg
     1381 gatgcggttt ttgccttttg cggtgcggcg tggacatcgg ctgctgccag caatgcggcg
     1441 gcaatcatca gggctttgat tttcatcatt ctttccttat cgtttgaatc ctgcctttga
     1501 tggcgggttt ggggttgtcg gtggaatgcc gtcttgtgcg ggaacggtgc tcctgccggg
     1561 tttccgacgg tcagggccgt ttgttgcgtc cggcggaaag agagtgggcg gggatatagc
     1621 cgggaacggc gacatccatc ctgtctgcgg ggtttctgcc gacactgctg aggtctttca
     1681 actgaagtga gaaaaagaca gcgtttttgt aggtgttttc gccggtaacg tagcgttggg
     1741 cgtacacgcc cgcgccccag cagccgcaac tgcttttgta ttccgcaccc gccagcacct
     1801 ctatcggttt tttggcttca aaaccgtagt tgtaacggac gacggccgac aggttgcgcg
     1861 tcagcggcca ttgtgcggac aggtcgagct ggctgagttt gtcgtaaaaa taggaaccgt
     1921 cggacttcag gtagattttt tcgttgcgcc cgtatttgta gcgggcgttc agcactttgc
     1981 cctgtgcggg acggtagctt gcaccgacgg cgtagttctc ggcgcgtttg tcgttttggt
     2041 tgtagtggat gctgctgtcg aggatgaagc ggctgccgat gccgctggag gcgaatgcca
     2101 cccagtcgga acggctgcgc ggttttttgc cgacactgcc gtcaagcatg actgcgtcgt
     2161 ttttgaagta gaatttctgc ccgatgccgg cgcggaaacg ctcttccccc gtcgcgccgt
     2221 ccaaaatacg gctttgcacg gcggcggaaa ggctgtttgc ggtgttaatc ctgtcgttgc
     2281 cgtaatagag gttttcacga aaaagctgcc cgtagccgaa gctgctttcc gacgaatcga
     2341 aattgggcag gtcgttttgg gatttggcag gaatatagtt gtagaacagg cgcggctcga
     2401 gggtttgcag gactccgccg ccgaacatcc gcgtattgcg ttcgaaggtc atgccgctgt
     2461 cgatgttgac gatgggcaga gtgcggctga cgcgtcgggc ttcttggctg ccgaagcggt
     2521 tgaggctgta ataggtggcg tgcagtccga gtttgggacg gacgtaaccc cagctgttgc
     2581 tgaaatccca tttgatgtcg ggatagacga cgaggcggct gccgtcttgg cggctgtcgt
     2641 ggctgaagcg ggtaaattgg gcggacacgc cgatttgcgc cctgccggtg tttttgcgcc
     2701 aatcggcgga aaggcgcggc atcagggcat acggtttgtc tttgtagccg ctttggtttg
     2761 ccagcgtctg gtatttcaga accgaaaggc cggcattcag gctgccgccc gccgccctgc
     2821 cgccataatc cagccataca cggcggttga ggttgacgtt gccggcgatt tctttgttgc
     2881 cgtaaaagtc gcggtagtag ccgctgtcgg agacttggtt gaaatcgaca cccgcctgaa
     2941 gcgtgtcgga aatgtcgtgc cggtgctgcc atttcgcctg atagcggtta ttcctgccgc
     3001 ttttcttgtc gtgcggcagc caggtcaggt cggactggcc ggcataatcc ggccgcaggt
     3061 agcgtacctg cccgtcaaag accgcgccgc gttcgccgat cacgccgggc gcgaacgtgg
     3121 catcgagatt gggggcaagg ttgaaataat agggaacgga aagggaaacg ccgtccgaac
     3181 cggcggacag tgagggaacg agcaggccgc ttttgcggtt gccgtcaagc gggaagtccg
     3241 cccaaggggt gtagaaaatg ggaacgccgc cgaacacgaa ggcggcgtgt ttggcaacgc
     3301 ctatgccttt ttcccgatcg gcttcgacgg aagcggcctt gacataccag ccggcatcgc
     3361 cggcggaaca ggtgttgaat tgggtttccg tcagtttgta atgcccttcg cccaacattt
     3421 cggcggtgcg gctgacgctt tgcagccgcc gtccgccgtg ttcggtttcc atacggacgt
     3481 tgtgcgcttc cccggtctgc tgctcgagat tgtaggtcag ggtttcgccc cgaatcagcg
     3541 taccgtcctg ttggagggcg aaccggtcgc ctgcggtaac ggtgtcgccc gactggtcgt
     3601 aatccgccca atcggcattg agggtcgtcc gattgcgttc gacgacgacg ttgccttcgg
     3661 cacgcacctg cacctgcgac tgtccttcca tcctgtcggc aacaatgcgc gtatagtctt
     3721 cggggatgga tgcttcgccg ctgccttgga cggcggcttc ggttctctcg gggctgccgc
     3781 tttcgttact gcaaaacagg caggtcgaac cgaggctcag gctggtaggc tgtatgggtt
     3841 cggacacgct ccgaacgctt cctccggcgg tcggattgtc cgtttcctcc gccgcaacgg
     3901 catcggcggc ggcgcaatgc gtgccgaagc agaggcccaa tgccagcacc agtggtttga
     3961 gtgaaaataa acgagccaaa atcgcccctc aagtcggttt gccagttaga atagcgttta
     4021 ttgtaacctg aaatgcttta gtactgttat gcaacggcaa atcaaactga aaaattggct
     4081 tcagaccgtt tatcccgaac gggacttcga tctgactttt gcggcggcgg atgctgattt
     4141 ccgccgctat ttccgtgcaa cgttttcaga cggcggcagt gtcgtctgca tggatgcacc
     4201 gcccgacaag atgagtgtcg caccttattt gaaagtgcag aaactgtttg acatggtcaa
     4261 tgtaccgcag gtattgcacg cggacacgga tttggggttt gtggtattga acgacttggg
     4321 caatacgacg tttttgaccg cgatgcttca ggagcagggc gaagcggcgc acaaagccct
     4381 gcttttggag gcaatcggcg agctggtcga attgcagaag gcaagccgtg aaggggtttt
     4441 gcccgaatat gaccgtgaaa cgatgctgcg cgaaatcaac ctgttcccgg aatggtttgt
     4501 cgccaaagaa ttggggcgcg aattaacatt caaacaacgc caactttggc agcaaaccgt
     4561 cgatacgctg ctgccgcccc tgttggcgca gcccaaagtc tatgtgcacc gcgactttat
     4621 cgtccgcaac ctgatgctga cgcgcggcag accgggcgtt ttagacttcc aagacgcgct
     4681 ttacggcccg atttcctacg atttggtgtc gctgttgcgc gatgccttta tcgaatggga
     4741 agaagaattt gtcttggact tggttatccg ctactgggaa aaggcgcggg ctgacggctt
     4801 gcccgtcccc gaagcgtttg acgagtttta ccgctggttc gaatggatgg gcgtgcagcg
     4861 gcacttgaag gttgcaggca tcttcgcacg cctgtactac cgtgacggca aagacaaata
     4921 ccgtcccgaa atcccgcgtt tcctcaatta cctgcgccgc gtatcgcgcc gttatgccga
     4981 actcgccccg ctctacgcgc tcttggtcga actggtcggc gatgaagaac tggaaacggg
     5041 ctttacgttt taaacccaat caaaatgccg tctgaaaacc aagtttcaga cggcattttt
     5101 caaacgggct tactgcgcgg ctttttgttc ttcgcgtact ttgtccgcca aaaggtcgat
     5161 ggtgttcata ccggactccc agtcggcaaa ttcaacttta tatttaccgc cgacgataac
     5221 cgtgggcgta ccgtcgattt ggaaggtttc ggtcagctcc tgcattttgt cggcgcgcgc
     5281 ctggctttcg ggggactcgt aggcggcaag gacttttttg ccgtcaaagg cggtttgttc
     5341 gcccagccat tttttgagga cttccggatt ttgcagcttg attttttggt tgaccatcgc
     5401 atcgaaaata tggctgttcg ccacatcttt gctgtcggcg gcagccatat cgacggcggc
     5461 ggcgaggcgt gccagcggca gcatttcttt ctgccagacg acgtgctcga tacgcaggta
     5521 catatcgtct ttaaaagact tggcgtgttt gctcaaaaca ggttcgaggt gggcgcagtg
     5581 cgggcagaaa tagccgaaaa actcaaggac ttcaaccttg cctgcctgct gttggggaat
     5641 cgggttggca aggacggtat agttttgccc ttcgaccagt ccggcggggg cggcggctgc
     5701 cgaagcagca ggcgcgctgt cggcggggac gctggtttgg actttgctgt cgcacgcggc
     5761 aagggcgaac agggcggcaa cgccgagggc gaggtgtctg gatttcatac ggctctccgt
     5821 gatgttggaa ataaatcgga tattggtatt ttattgcatt tccgcgtgtt gatacagttt
     5881 gccgccggaa aaggacgttt tcgtttgggg aaaccgcttc ggacggcatc aaacccgatg
     5941 ccgtctgaag cggtttctgt cgtacaatac gcgccgtcgc cccagacgga tacggctgtt
     6001 ggggaactaa tgatgttcca acacacagga cggcacataa agcaccgccc tatgcgttgc
     6061 cctggttcgg aagggttaca cccctttcaa atatagtgga ttaaatttaa accagtacag
     6121 cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
     6181 taaatttaat ccactatcaa agtctgatcc tgccgcccta aagggcgggg tttcaaccga
     6241 aaaggaaata cgatgaatat gctgggagct ttggcaaaag tcggcagcct gacgatggtg
     6301 tcgcgcgttt tgggatttgt gcgcgatacg gtcattgcgc gcgcattcgg cgcgggcatg
     6361 gcgacggatg cgttttttgt cgcgttcaaa ctgcccaacc tgcttcgccg cgtgtttgcg
     6421 gagggggcgt ttgcccaagc gtttgtgccg attttggcgg aatataagga aacgcgttct
     6481 aaagaggcga cggaggcttt tatccgccat gtggcgggga tgctgtcgtt tgtgctggtc
     6541 atcgttaccg cgctgggcat acttgccgcg ccttgggtga tttatgtttc cgcacccggt
     6601 tttgccaaag atgccgacaa atttcagctc tccatcgatt tgctgcggat tacgttcccc
     6661 tatatcttat tgatttcact ttcctctttt gtcggctcgg tactcaattc ctatcataaa
     6721 ttcagcattc cggcgtttac gcccacgttc ctgaacgtgt cgtttatcgt attcgcgctg
     6781 tttttcgtgc cgtatttcga tcctcccgtt accgcgctgg cttgggcggt ttttgtcggc
     6841 ggtattttgc agctcggctt tcagctccct tggttggcga aactgggttt tttgaaactg
     6901 cccaaactga atttcaaaga tgcggcggtc aaccgcgtca tgaaacaaat ggcgcctgcg
     6961 attttgggcg tgagcgtggc gcagatttct ttggtgatta acacgatttt cgcgtcttat
     7021 ctgcaatcgg gcagcgtttc atggatgtat tacgccgacc gcatgatgga gctgcccagc
     7081 ggcgtgctgg gggcggcact cggtacgatt ttgctgccga ctttgtccaa acactcggca
     7141 aaccaagata cggaacagtt ttccgccctg ctcgactggg gtttgcgcct gtgcatgctg
     7201 ctgacgctgc cggcggcggt cggactggcg gtgttgtcgt tcccgctggt ggcaaccttg
     7261 ttcatgtacc gagaattcac gctgtttgac gcgcagatga cgcaacacgc gctgattgcc
     7321 tattcttttg gtttaatcgg tttaatcatg attaaagtgt tggcacccgg cttctatgcg
     7381 cggcaaaaca tcaaaacgcc cgtcaaaatc gccatcttca cgctcatctg cacacagttg
     7441 atgaaccttg cctttatcgg cccactgaaa cacgtcggac tttcgcttgc catcggtctg
     7501 ggcgcgtgta tcaatgccgg attgttgttt tacctgttgc gcagacacgg tatttaccaa
     7561 cctggcaagg gttgggcagc gttcttagca aaaatgctgc tctcgctcgc cgtgatggga
     7621 ggcggcctgt atgccgccca aatctggctg ccgttcgact gggcacacgc cggcggaatg
     7681 caaaaggccg cccggctctt catcctgatt gtcatcggag gctgcctgta tttcgcatca
     7741 ctggctgcac tgggcttccg tccgcgccat ttcaaacggg tggaaagctg accgatgccg
     7801 atatcctttt tcggcaaacg ctttttccac gcctatattc gggcgcgcat cccccgcgtc
     7861 cgcaccggcc ggacgggctg ccgcatcatc aatgccgtct gaaaacaaga aaaccgatac
     7921 cttatgattt tgacaccgcc ggacacgccc cttttcctcc gcaacggcaa tgccgacacg
     7981 attgccgcca aattcctgca acgccccgca cctgcatacc gccgcgagct gcttcccgac
     8041 agcacgggta aaaccaaagt cgcctacgac ttttcagacg gcatttcgcc cgatgcgccg
     8101 ctggtcgtgc tgtttcacgg tttggaagga agcagccgca gccattacgc ggtcgaactg
     8161 atgcttgcgg tacgcgatcg gggttggcac ggcgtagtcg tccatttccg cagctgcggc
     8221 ggcattgcca acaccgctcc ggtgttctac cacttgggcg ataccgccga aatcgccttt
     8281 actttggaca cgttcgccgc gcgttaccgt gaaatatacg ccgtcggcgt atcgctgggc
     8341 ggcaacgcgc tggcaaaata tttgggcgaa cagggcgaaa acgcgctgcc gcaagccgcc
     8401 gccgtcatct ccgcacccgt cgatgcagag gcggcaggca accgcttcga cagcggcatc
     8461 acacggctgc tctacacgcg ctacttcctc cgcacactga tacccaaagc acggtcgctc
     8521 caaggttttc agacggcatt tgccgcaggg tgcaaaacac tgggcgagtt tgacgaccgt
     8581 ttcaccgcac cgctgcacgg ctttgccgac cggcacgact actaccgcca aacttcctgc
     8641 aaaccgctgc tcaaacacgt tgccaaaccg ctgctcctgc tcaatgccgt caacgacccc
     8701 ttcctgccgc ccgaagcgct gccgacaccg gacgaagtgt ccgaaaccgt taccctgttc
     8761 caacctgcac acggtggtca tgtcggcttt gtcagcagca ccggcggcag gctgcacctg
     8821 caatggttgc cgcagaccgt cctgtcctat ttcgacagct tccgcacaaa caggcgttaa
     8881 cggtttgatg ctaatattcc ccctttccca gacgaatacg gaacacgaca tgaccgacat
     8941 cctcaataaa atccttgcca ccaaagcaca ggaagttgcc gcccaaaaag ccgccgtcaa
     9001 cgccgaacac atccgcgcac ttgccgcaga agccgcgccc gtccgcagct tcatcgattt
     9061 gatacgcggc aaacaccgcc taaacctgcc cgccgtcatt gccgaaatca aaaaggcaag
     9121 cccgagcaaa ggcctgatcc gtccggactt ccgaccggcc gagattgcac gcgcatatga
     9181 aaacgccgga gcggcatgtt tgtccgtact gaccgacgaa ccctatttcc aaggttcgcc
     9241 cgaatacctc aaacaggcgc gcgaagccgt atcgctgccc gtgctgcgca aagacttcat
     9301 catcgacgaa tatcaggttt atcaggcgcg cgcatggggg gcggatgccg tcctgctgat
     9361 tgccgcagca ctggaacagg aacaattgga acgctttgaa gcggtggcgc acgaattggg
     9421 catgaccgtc ctgctcgagc tgcacgatga aaccgaattg gaaaaatgcc gcaacctgac
     9481 cacgccgttg tggggcgtaa acaaccgcaa cctgaggact tttgaagtct ccctcgacca
     9541 aaccctgtcg ctgctgcccg cgctggaagg caaaaccgtc gttaccgaaa gcggcattac
     9601 gggtaaggcg gatgtggaat ttatgcggac gcgcggtgtg cacaccttcc tgatcggcga
     9661 aacgttcatg cgtgccgccg atattgaggc ggaagtgggc aaactcttct aaatcccgat
     9721 ttcagacggc atattgccgc cgaccgatca acacccttac cgagcacgga accatcatgc
     9781 accgaccgac cgccaaacag attttgcacg aagtattcgg ttatcccgaa tttcgcggca
     9841 agcaggagtc tatcgtcaat gctttggccg gcggcgagag tttgatggtg ctgatgccga
     9901 cgggcggggg caagtctttg tgttaccaga ttccggcgct gatgcgcgaa ggcgtggcag
     9961 ttgtcgtatc gccgctgatt gcgttgatga acgaccaagt ggccagtctg catgtggccg
    10021 ggattgaagc ggcggcagtc aacagcagca catcggcaga tgaggcgcgc gagattgccg
    10081 acaagcttgc ccaaggccgt ctgaaactgc tttatgtcgc gccggaacgc ttggttaccg
    10141 accgcttttt gcgttttctc gaccaacaaa ccgtcagcct gtttgccatt gacgaggcgc
    10201 attgcgtcag ccaatgggga cacgatttcc gccctgaata tcaacagctc ggcatccttg
    10261 ccgaacgcta tccgaacgtc ccgcgcatcg ccctgaccgc taccgccgat gccgccacgc
    10321 gcgccgacat caagcattat ctgcacttgg acaatgcgcc cgaatttgtc tccagcttcg
    10381 accgtccgaa tatttattat caggttatcg aaaaaaacaa cggcaaaaaa caattgctgg
    10441 atttcatccg caaagaaatg acggggcaaa gcggcattgt gtattgccta agccgcaaaa
    10501 aggtggaaga tgtggcgcag tttttgcgtg aaaacggatt aaacgcgatt ccgtatcatg
    10561 ctggtttgag catggacgtg cgcgaggaaa accaacgccg ctttacgcat gaagacaata
    10621 ttatcgtggt ggcgaccgtg gcgttcggca tgggcataga caaacccgac gtgcgctttg
    10681 tcgcccatct cgatatgccc cagagtgtcg aacatttcta tcaggaatcg gggcgtgccg
    10741 gccgggacgg gctgcctgcc gtgagctggc tgtgttacgg tttgaacgat tgggtgttgc
    10801 tgcgcgaacg gattgccgaa ggcaacagcg acgaggtgca aaagcaaatc gaaatgcaaa
    10861 aactcgatgc catgctttcc gtctgcgaaa ccgccgcctg ccgccgcgta ctgctgctca
    10921 aacatttcgg cgaagcatcc gaaccctgcg gccattgcga caactgcctg catccgcccg
    10981 tacggtttga cggcacggtg ttggtgcaaa aattactcag ctgcgtgtac cgcgccggac
    11041 aacgttttgc cgccggttac atcaccaacc ttttgcgcgg caaaagcgac gattggatac
    11101 gcggcaaccg gcacgaacaa ctgtccacct tcggcatcgg tacggagttg tccgacaaag
    11161 aatggcgcag cgtcatccgc cagtgtatca gcctcggcta cctcaccgtc aacattaccc
    11221 gatatcaggc attgcaactg accgaagccg ccaaaaaagt cctcaaaggc gaaaccgaag
    11281 tgatgctgcg tccgctcaag cgcgacaagc ccgccgcccg caccctcaaa gacaactggc
    11341 tgcgtaccga acgcgaagaa cgcctgtggc aggcattgcg cgtttggcgt atgaaacagg
    11401 cagaagccga agtcatcccc gcctatatga ttttcggcga caaaaccctg cgcgaccttg
    11461 tcgaaaaaat gccgcaagac ctcaacggac tgcacgacat ctacggtttg ggcgaagcca
    11521 aaatcgaccg tttcggacac ggcatcctcg aagtctgccg caacaccgcc gactttagcc
    11581 acaatgccgt catccgcccg caaaccgaac gcgaacaaca actgcgtcaa aaactcgaag
    11641 cctggcggta tgaacaggca agggcggaaa actgcgccct gcatgccgtc ctctccgacg
    11701 aaagccttgc cgatatgctt gccgatacgc ccgaaaccga aaccgacctc gaaggcgtac
    11761 acggcttggg cagcgtacgc gccgccaaat acggacggga catcctcgcc gtctgccgtc
    11821 cgttttcaga cggcatcgat gaagccgcca aacacaaacg ccgcctgatg cgcgccctga
    11881 ttcaatggtg caacgaaacg gcaaaacacg aacagtccga accctaccgc attctcagca
    11941 aagccgccct gcgcgccatt gccgccaaac agccggaagg tttggcggag cttgccgccg
    12001 tatacggcgt aggcgaagaa aaagccgcac gttacggtgc ggcggtgttg gcggtattgg
    12061 aacgaaatgc cgtctgaaaa ccgttaccaa gtttcagacg gcattgcctc tatttaaaaa
    12121 ttcctg
//
LOCUS       DAWFIE010000062        12062 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWFIE010000062 DAWFIE010000000
VERSION     DAWFIE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.062"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            139..573
                     /locus_tag="WGQ71_001536"
     CDS             139..573
                     /locus_tag="WGQ71_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ0707523.1"
                     /translation="MNFALSVITFTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(622..1965)
                     /locus_tag="WGQ71_001537"
     CDS             complement(622..1965)
                     /locus_tag="WGQ71_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ0707524.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLVSDGIPLAGAWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1970..2623)
                     /locus_tag="WGQ71_001538"
     CDS             complement(1970..2623)
                     /locus_tag="WGQ71_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ0707525.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIVAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2774..3064
                     /gene="gatC"
                     /locus_tag="WGQ71_001539"
     CDS             2774..3064
                     /gene="gatC"
                     /locus_tag="WGQ71_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ0707526.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            3127..4572
                     /gene="gatA"
                     /locus_tag="WGQ71_001540"
     CDS             3127..4572
                     /gene="gatA"
                     /locus_tag="WGQ71_001540"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ0707527.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEAGTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4569..5500
                     /locus_tag="WGQ71_001541"
                     /pseudo
     CDS             4569..5500
                     /locus_tag="WGQ71_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
     gene            5543..6973
                     /gene="gatB"
                     /locus_tag="WGQ71_001542"
     CDS             5543..6973
                     /gene="gatB"
                     /locus_tag="WGQ71_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ0707528.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPELR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7065..8075
                     /locus_tag="WGQ71_001543"
     CDS             7065..8075
                     /locus_tag="WGQ71_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ0707529.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLSAAARQNLNLPHSCKNGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCRTTAQSDISLNIPGYNPNALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQDSSRAVHFYWGARHQDDLYALKEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQTKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8204..8836)
                     /gene="pdxH"
                     /locus_tag="WGQ71_001544"
     CDS             complement(8204..8836)
                     /gene="pdxH"
                     /locus_tag="WGQ71_001544"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ0707530.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLLEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            8896..9051
                     /locus_tag="WGQ71_001545"
     CDS             8896..9051
                     /locus_tag="WGQ71_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707531.1"
                     /translation="MHKLCLATSRSDDFIRKTAASGTISNRPTEDGILSTRPAAHAAE
                     EPPYQAS"
     gene            9060..10109
                     /locus_tag="WGQ71_001546"
     CDS             9060..10109
                     /locus_tag="WGQ71_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0707532.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETGKTASQPY
                     GQKASDGIKLQNTPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(10178..11719)
                     /locus_tag="WGQ71_001547"
     CDS             complement(10178..11719)
                     /locus_tag="WGQ71_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ0707533.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVSTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVVETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSVGRQASVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAARSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
BASE COUNT         3084 a         3658 c         2995 g         2325 t
ORIGIN      
        1 tgggaatctg tttcacccca atatataaag caccatatta aggcggagtt tcttccccac
       61 tttgacccga acccggaaaa gacaccgccc aagccaatcc tgatgctgcc ccgacagcca
      121 acccttaagg aaaccctaat gaactttgct ttatccgtca tcacatttac cctcgcctct
      181 ttcctgcccg tcccgcctgc cggagccgcc gtctttactt ggaaggacgg cggcggcaac
      241 agctattcgg atgtaccgaa acagcttcat cccgaccaaa gccaaatctt aaacctgcgg
      301 acgcgccaaa ccaaaccggc ggtcaaaccc gcccaagccg acgcagggaa gcgcacagac
      361 ggcgcggcac aggaaaacaa tcccgacact gccgagaaaa accggcagct tgaggaagaa
      421 aagaaaagaa ttgccgaaac cgaacggcag aacaaagaag aaaactgccg gatttcaaaa
      481 atgaacctga aagcggtggg aaattcaaat gcaaaaaaca aggatgattt gattcggaaa
      541 tacaataacg ccgtaaacaa atactgccgt taatcggctc tagcgcaaac cccatgccgt
      601 ctgaaacggc acggggtttg tttatttctg ccagtaggtt ttgacgttga cgaactcgta
      661 cagcccgaat tcggacaatt cgcgcccgta accggaatct ttgactccgc cgaaaggcag
      721 gcgcaaatcg ctgctggtat ggcggttgat aaacaccgat cccgcctgta ttttttcggc
      781 aaaccgccaa gcgcgttcgg tatcggcggt ataaatgcag gcacctagcc cgaacgggga
      841 atcattggca agggcgatgg catgttcttc gttttcggcg cgcaaaatca gggcggccgg
      901 cccgaatact tcttctctcc agacgcggca ggcaggattt accctgtcta aaaccgtcgc
      961 gggataaaac cagcctcgcc cttgtgggat ttttccgccg gtcaggcata ccgcgccgtt
     1021 tgaaacggca tcttcaacct gcccgtgaac cctgtcccgc aaatcttcgc ggtgcagcgg
     1081 tgcaagcgta gtatcgggat gtttggggtc gcccattttc aatttggcgc attcggcaag
     1141 aaacagcgtg ataaaacgat cggctgcggc ttcggttacg atgatgcgtt tggcggcgtt
     1201 gcacgattgc cccgcatcgc ggaaacggga ataacaggct tctgcggcgg cgcgttccaa
     1261 atcggcatcg ggcatcacga taaaggcgtt gctgccgccg agttccaaga cggttttctt
     1321 gaggtttgcg cccgcatgtg ccgcaaggat gcgccccgta tgcgtcgagc cggtaaacgc
     1381 catcgcatcg gtatcttcaa ccgccttgag cgtgcccgcc tcatccaacc acgcgcctgc
     1441 caaaggaatg ccgtctgaaa ccaaatcaaa caacgcctga ctgacgcgtg ccacgctggg
     1501 cgcgggtttg acggcgcacg cgttgcccgc gcacatagcg ggaacggcga aacgcaatac
     1561 ctgccagacg ggatagttcc aaggcatgac ggcaaacacc acgcccaaag gctcaaacct
     1621 tacctgactc aaactcgcct gcgtcgcgat ggttttgtgg gcgagcagtt cgggagcgag
     1681 gcgggcgtaa tagcgtatca gttcgataga cttgccgatt tccgcacggc attcgtgcag
     1741 gcagcgtccg acttcttcgc acaccatttc cgcaaaacgc tctttctccg cctccaaacg
     1801 gtcggcaaat ttttgcagac gcgcggcacg ttcggttacg cccagttgcg cgaacgcccc
     1861 gccgcgcatt ttcaaatccg ccagccgccg ttcaaactcc gcataatctt gagctggacg
     1921 gcggtaaagc gtttcgcccg taaatacatt gacactgtga aacatcgaat caacctgcca
     1981 gttgcgggaa tatcgttttc agtcccgaca caataatctc caccgatacc gccgccagca
     2041 tcatacccat gatacggttt aaaatcgtca gccccgtcgc gcccagcagg cggctgacct
     2101 tccctgcaac aattaaaatg gcataacaaa tcgcactgac caccaaaccg gccacgataa
     2161 tcagcgcgat gtcgctgtac gttttggctg ccgaagcgta aataatcacg gtcgaaatac
     2221 cgcccgggcc gatggtgatc ggtatggcga tgggcacgac ggcaatcgct ccggcattgc
     2281 gggcggggcg cgcctgcccc gtttccggct gcgcgccgag attctgcttg gcgggattgt
     2341 cgttgccgtt catcatcgaa atggcgatca gcagcaccaa aatcccgccg ccgacctgaa
     2401 acgaaccgac gctgatgccc aaaaccttca gcagcgtacc gccgatcagc gcaaataccg
     2461 caatcacggc aaacacggca acggcggccg tccgcgcgac cttcctgcgc tccttcgtgc
     2521 tgtgcccgtt ggtcaggtca aggtaaagcg acaacgcgct aaacggatta atcagcacca
     2581 aaaaagccac aatcagcttg ccgatttcca tgcccaatcc cattatttcc ccctccttca
     2641 aacccgtgcg gcaggcatcc gatgctgcaa attgccgccg caacggattt ttccgttata
     2701 attaaaaatt caagcaatac gccccatcat acccgaacga cggtatcttt accatcagac
     2761 aaggatgctt ttcatggcac tgacacttgc cgacgtagac aaaatcgccc gactctcccg
     2821 actgcacctg actgcggaag aaaaagaaaa atcgcttcaa gaattaaacg acattttcac
     2881 tatggtcgaa cagatgcaaa ccattaacac agacggcatc gaaccgatgg cgcacccgca
     2941 cgaggccgcc ctgcgcctgc gcgaagacga agtaaccgaa accgaccgcg ccgccgaata
     3001 tcaggcgggt gctccggaag tacgcaaccg tctgtacatc gtaccgcaag ttatcgaaga
     3061 ataatccgaa tatgcttcag acggcatcag caataccgcc cgaagccctt taaggatgga
     3121 agatttatga cccaatacac attgaaacag gcaagcgtcc tgttgcagtc caaacagatt
     3181 tccgccgtcg aactggcaag cgcatacctt gccgccatcg ccgaaaaaaa tcccgccctc
     3241 aacggctata tcaccatcga ccaagataaa acccttgcag aagcccgtgc cgccgacgaa
     3301 cgtatcgcgc agggcaacgc ctccgcgctt accggcgtac ccgtcgccta caaggatatt
     3361 ttctgccaaa ccggctggcg cagcgcgtgc gcttccaaaa tgctcgacaa cttcatctcc
     3421 ccctacaccg ccaccgtcgt ccaaaacctg ctcgacgaag gtatggtaac gctcggccgc
     3481 accaatatgg atgagttcgc tatgggttcg accaatgaaa actcattcta cggcgcggcc
     3541 aaaaatccgt ggaatccgga acacgtcccc ggcggttcgt caggcggttc cgccgccgtc
     3601 gttgccgctc gccttgcccc tgccgcgctc ggttcggata ccggcggctc tatccgccag
     3661 cctgcatcgc actgcggcat taccggcatc aaacccacat acggcacggt ttcccgcttc
     3721 ggtatggtcg cctacgcctc cagcttcgat caagccggcc cgatggcgca aactgccgaa
     3781 gactgcgcga ttctgttaaa cgcgatggca ggtttcgacc cgaaagactc caccagcctc
     3841 gagcgcgaaa aagaagacta cacccgcgat ttgaaccaac cgctcaaagg tttgaaaatc
     3901 ggcctgccca aagaatattt cggcgaaggc aacagcgccg atgttctgac ggcattgcaa
     3961 aacaccattg atttgctgaa agcccaaggc gcggaattga ttgaagtttc cctgccgcaa
     4021 accaagctgt ccatccccgc ctactacgtc ctcgcctccg cagaagccgg caccaacctt
     4081 tcacgttacg acggcgtgcg ctacggacac cgtgccgccc aattcggcga tttggaagaa
     4141 atgtacggca aaacccgcgc cgaaggtttc ggcagcgaag tcaaacgccg catcatgatc
     4201 ggcacttatg tactgtcgca cggctactac gatgcctact atctcaaagc ccaaaaactg
     4261 cgccgcctcg ttgccgatga ttttcagacg gcatttgcac ggtgcgacct catcctcgcg
     4321 ccgaccgcac ccactgccgc ccccaaaatc ggagcggatg cttcgccggt tgaaacctac
     4381 ttgagcgata tctataccat cgccgtcaac ctcgcaggac tgcccgcatt gaccctgccc
     4441 gcaggcttca gcggcggcgg actgcccgtc ggcgttcagc ttgtcggcaa ctacttcgcc
     4501 gaagccaaaa tcctcggtgc ggcgcatcaa atccaactca acagcgattg gcacggcaaa
     4561 cgacccgaat gaagcagaac cgcaccttta ccttccccga ttttcgcacc gtttacagct
     4621 atgcgccttt atatcggctg caacatttaa aatacacatt gcgaaaattt ttcggaaaaa
     4681 aagaaattta cgccttcgag cagtttgtca acgcatcccc tatccgtcag gggctgttcc
     4741 tccactgccc gcaaaatgcc tatccgctgc tgcgcgaatt tgttgacagg cgttttaact
     4801 gcaaacgccg tttagatgcg atgacggcag attttctcat ggcggaaaaa ctgttcggca
     4861 cagacatcct gcaccaaatg gaagactacc gcttccatct ggtcttggcg cacctttcag
     4921 acggcatcag cttgtggctc aaccgcaacg acaactgcgt cgaagaaggc gcgtggtctt
     4981 tatctttgcg cgacgaagca ggcaaccggc tgtatatggc gaccttcgcc tttgtcggca
     5041 cacacctgct gacagcctcc gtacaagggc cggcgggtga agaagccaaa gacaccgtcc
     5101 gccgcataac caaacaactt cacggcttgc gtccccaaca actgatggta accgccctgc
     5161 aatatttcgc cgccgtactc ggcttggacg gcgcaatggg cattgcacaa aaacatcagg
     5221 tcaaactgcg ctggaaactt aaaaagcgtg tcaaaatgaa ttacgacgca ttctggcagg
     5281 aatacggcgc aagtttggaa cgggacggct actggcatct cccccaaacc cccgcccgca
     5341 aagaccttac cgacatcgaa agcaaaaaac gctcgatgta ccgcaagcgt tatgaaatgc
     5401 tggacaacat ggttgcagag atgaaggacg gtctgaaaca gaagcacgcg gcatttcaga
     5461 cggcatccaa acggaaaaac cgctccgccg gacagcctga cgcgaagact atcgaattga
     5521 tattttagag aaagaagctc ttatgacctg ggaaaccgta atcggcttgg aaatccacgt
     5581 ccaattgaac accaaatcca aaatcttcag cggcgcatcg accgcattcg gcgcagaacc
     5641 caacgcgcac gccagcgtgg tggaatgtgc tttgccgggc gtactgccgg taatgaaccg
     5701 agaagtcgtt gaaaaagcca tcaaattggg tttggctttg gatgcgaaaa tcaaccggaa
     5761 aaacgtgttc gaccgtaaaa actacttcta tcccgactta ccaaaaggct atcaaatcag
     5821 ccagttggac ttaccgattg tcgaacacgg caaattggaa atcgtagtcg gcgacgatgt
     5881 gaaaaccatc aacgtaaccc gcgcccatat ggaagaagac gcgggcaagt ccgtgcatga
     5941 aggcttgaac ggcgcgaccg gtatcgacct gaatcgcgcc ggcacgcctt tattggaagt
     6001 cgtatccgaa ccggaattgc gctccgccgc cgaagccgtt gcctacgcca aagccttgca
     6061 cagcttggta acctggctgg acatttgcga cggcaatatg gcggaaggtt cgttccgcgt
     6121 cgatgccaac gtatccgttc gcccgaaagg tcaagcggaa ttcggcacgc gccgcgagat
     6181 taaaaacctc aattccttcc gtttcttgga gcaggcgatt aattacgaag cagaagcgca
     6241 aatcgagatt ttggaagacg gcggcaaagt acagcaggca acgatgctgt ttgatcccga
     6301 aaaaggcgaa acccgcgtaa tgcgcctgaa agaagacgcg caagactacc gctacttccc
     6361 cgaccccgat ttgctgcccg tcatcatttc agacgctcaa atgcaaaaag ccaaagcaga
     6421 aatgcccgag ctgccgaaag aaatggcagc gcgtttcgtg gcggactacg gcgtgtccga
     6481 atacgacgcg cgcctgctca ccgcaagccg cgcacaggcc gcctattttg aagaagccgc
     6541 caaagagagc ggacaaggca agccgactgc caactggatg aacggcgaac tcgccgccgc
     6601 actgaacaaa gaaggcatgg aacttgccga cagcccgatt accgctccgc gcctcgccgc
     6661 gctggtaggc aaaatcgccg acggcacatt aagcagcaaa ttagcgaaaa aagcctttga
     6721 agccatgtgg gcagaacccg aagccactat tgccgaaatc atcgaaaaac acggtttgca
     6781 acagatgacc gataccggcg cggttgaagc gatggtggac gaagtgctgg caaacaacgc
     6841 caaagccgtg gaacagttta aatccggcaa cgaaaaagcc ctgaatgcga ttgtgggaca
     6901 agtgatgaaa gccagcaaag gcaaagccaa ccccgcgcag gttcaagagc tgattaaagc
     6961 caaacttgcc tgataagcaa gatgccgtct gaaacccaaa tccgacgttc agacggcatt
     7021 ttcacagtct gaccacatta aaacagcata aaaaacaata ttccatgaac cacaccgtta
     7081 ccctgcccga ccaaaccacc tttgccgcca acgacggtga aaccgttttg tccgccgccg
     7141 cccgacaaaa ccttaatctg ccccattcct gcaaaaacgg tgtctgcggg caatgcaaag
     7201 ccgaactggt cagcggcgat attcaaatgg gcggacactc cgaacaggct ttatccgaag
     7261 cagaaaaagc gcaaggcaag attttgatgt gccgcaccac cgcgcaaagc gacatcagcc
     7321 tcaacatccc cggctacaac cccaacgccc tccccgtccg caccctgccc gcacgcatcg
     7381 aaagtatggt tttcaaacac gatgtcgccc tcttgaaact tgccctgccc aaagccccgc
     7441 cgtttgcctt ctacgccggg caatacattg atttactgct gccgggcaac gtcagccgca
     7501 gctactccat cgccaatccg cccgaccaag aaggcatttt ggaactacac atccgcaggc
     7561 gcgaaaacgg tgtctgctcg gaaatgattt tcggcagcga acccaaagtc aaagaaaaag
     7621 gcatcgtccg cgttaaaggt ccgctcggtt cgtttacctt gcaggaagac agcggcaagc
     7681 ccgtcatcct gctggcaacc ggcacaggct acgcccccat ccgcagcatc ctgctcgacc
     7741 ttatccgcca agacagcagc cgcgccgtcc atttctactg gggcgcgcgt catcaagatg
     7801 atttgtatgc cctcaaagaa gcacaagggt tggcatgccg tctgaaaaac gcctgcttca
     7861 cccccgtatt gtcccgcccc ggagagggct ggcagggaag aaaaggccac gtacaagaca
     7921 tcgcggcaca agaccacccc gacctgtcgg aatacgaagt atttgcctgc ggttctccgg
     7981 ccatgaccga acaaacaaag aatctgtttg tgcaacagca taagctgccg gaaaacttgt
     8041 ttttctccga cgcattcacg ccgtccgcat cataattccc cggtataaaa aagattcagg
     8101 ctttccgttc ggaacacaaa aaacttcccg tccgtatttt ccccataaaa aatgccgtct
     8161 gaagcccgat tccggttttc agacggcata tgttttttcc tgttcaaggc gacagccgct
     8221 cgcgtatcca gccaccatcc agcaaacggt attggatgcg gtcgtgcaac ctgctcggcc
     8281 tgccctgcca gaactcaagc aaatcgggaa tcacaatata gccgccccaa tgcggcggac
     8341 gcggcacgtg caaaggatgt ttgagtccga ccgccgccgc ctttgccacc aataccgcct
     8401 tgttcggaat cacttcgctc tgcgcgcttg cccacgcacc caaacggctc tgatacgggc
     8461 ggctctcaaa atattcgtcc gacaatttct ccgccagcct ttcaacacgc ccttccacgc
     8521 gcacctgacg ctccagctcc ggccaaaaaa acgtcatcgc cgcaaatgga tgagcatcca
     8581 gcgaacgccc cttgcggctg tgataattcg taaagaaaac aaaaccttca gaattaactt
     8641 ccttcagcag caccatacgg ctgttaggcc tgccgcgtcc gtcaaccgcc gccacattga
     8701 ccgccgtcgg ctcgttgacc tgtgcgcgta ccgcctcgtc caaccaccgc tcgaactgct
     8761 cgatcggatt gtcggcgcaa tcgccttccg acaattcccg tttgctgtaa tcttcccgaa
     8821 tattgtgcaa atccatttac cgctcctctt gtcatttgaa agattctacc cccgcacgca
     8881 aaccgatttc aaccgttgca caaactttgc ctcgccacaa gccgcagcga cgatttcatc
     8941 cgcaaaaccg ccgcatcagg tacaatatcg aaccgtccga ctgaggacgg cattttatca
     9001 acccgtcctg ccgcacacgc cgcagaagaa ccgccttatc aggcgagtta ggaaaaatga
     9061 tgtccaagca acccaccagc aaacgccaat ggcgcgacgg cgcagccccg tctgccaaga
     9121 aaaccgccaa acctttcaaa agcaaagccc gtcccaaaga cgaaacgggc aaaaccgcat
     9181 cccaacctta cggacaaaaa gcttcagacg gcatcaagct gcaaaacacc cccaaacaac
     9241 gcgccgccaa agccaaaaaa ctcgtcgtcc gcaatcccaa ccaaaagatt atggaacacg
     9301 cgcgcgattt gaaagaacgc cgcagcgacc tgtcgcgcat ggaacccgaa cgcctgcaaa
     9361 aagtgcttgc cgcgtccggc gtcggctcgc gccgcgaaat ggaagaatgg atcaacaacg
     9421 gctgggtaac ggtcaacggc aaaaccgcgc aactgggcga caaagttacc cccgacgacc
     9481 acgttaccgt caaaggcagt atcatcaaac tcaaatgggc ggaccgcctg ccgcgcatca
     9541 tcctgtatta caaacaagaa ggcgaaatcg tttcccgcga cgatccgcaa ggccgcgtca
     9601 gcatattcga ccgtctgccg caggccgcca gcagccgctg ggtcgccatc ggacgcttgg
     9661 acatcaacac cagcggactt ctgattctca ccacctccgg cgaactcgtc caacgtttcg
     9721 cccaccccag cttcgaagtc gaacgcgaat acgccgtgcg cgtcttgggc gggctgaccg
     9781 gcgaacaaat gcgcgtcctc accgaagaag gcgtgatgct cgaagacggc ttggcaaaag
     9841 tcgaacgcat ccgcgaacaa ggcggcgaag gcgcgaacaa atggtacaac gtcgtgatta
     9901 aagaaggccg caaccgcgaa gtgcgccgca tttttgaaag ccaaggactc accgtcagcc
     9961 gcctcgtgcg cgtcggcttc ggccccatcg gactgcccaa ccgcctcaaa cgcgggcagt
    10021 tctacgaact caaccccgcc gaagtcgcca acatcatcaa atgggcggac atgctgctgc
    10081 cgggcgaacg ccgccgcaaa aaagcctaaa cccgccaaaa cacaaaaatg ccgtctgaaa
    10141 catctactgt ttcagacggc attttattcg ggcgttttca ggagaaaagg tcgagtgctt
    10201 tgacaaagac catcaccacg ccgtaggcga gcggtatgac ggccaatgcc caacgccacc
    10261 agaccgatat gccgccgccg gaaacttttt cgcccacaag gtaagcgtcg gatatggcgg
    10321 tttcgtcatc ggggttgccg ctgcgtgcgg cggttttgat gtctttttcg tggtgttttt
    10381 cgtgtacgga tttgacggcg aggttgcaca acaaaccgat aatcagcaga cccgccatga
    10441 tgtacatggt tacgctgtat gcctgtgccg ccggtatgcc gctgtcgatt tggctttggc
    10501 gtatgtaatt gaccagtacc gggccgatga cggcggcggt tgaccaggcc agcaggatgc
    10561 gcccgtgaat cgcgccgacc tgataggtgc cgaacaggtc tttcaggtag gcgggaatgg
    10621 cggcaaatcc gccgccgtac atggaaataa tcacgcaaaa gccgatgatg aacagggctt
    10681 tgctgccgcc ctcgccgatg gagggaacgg cgaaatacag cagcgaaccg agtacgaaga
    10741 aaatggtgta ggtgtttttg cgtccgattt tgtcggaaac gctcgaccat aaaaagcgtc
    10801 cgcccatgtt aaacaggctc aggaggctga cgaagcctgc cgccgcacct gcgccgactg
    10861 acgcctgcct gcctacggag gtttcggaaa agagttcctg aatcatcacg gatgcctgac
    10921 ccaatacgcc gatgccggcg gttacgttca ggcacaatac ccagaacaac agccaaaatt
    10981 gcggcgtttt catggcttgt gacacgttga catgattgct gctgaccagc ttgttttgcg
    11041 ttttcggcgc ggtatagcct tcaggtttcc agccgtcggc aggtacgcgg atggtaaacg
    11101 cgccgaacat catcagtgcg aggtaaagca ggcccaatac ggcgaaggtt tcaacaaccc
    11161 cgactgaagc ggcgtttgaa aaggcgttca tcagtgatac ggaaagcgga gaggccagca
    11221 ttgcgccgcc gccgaaaccc ataatcgcca aaccggtcgc catacccggc ttgtcgggaa
    11281 accatttcat cagtgtggaa accggcccga tgtagcctaa gcctaagcct acgccaccga
    11341 tgacgccgtt gcccaaataa agcaggaaga ggttgtgcgt actcacgccg aatgcggata
    11401 cgaagaagcc caggctgaaa cagcaggcgg cggcaaatat ggctttgcgc ggccctaccc
    11461 gttccatcca tgtgccgaac agggcggccg acgcgcccag catcgcgagt gcgatactga
    11521 aaatccaacc tacggtcgtc agcttccaat ctccggccgc cgattcggtt atgccgataa
    11581 gtttggtcag cggcgcgttg aatacggaat aggcgtaaat ctgcccgatg gcaaggtgca
    11641 ccgccaatgc tgcgggcggt acgagccaac ggttgaaacc cggcttggca atgcttgcct
    11701 cacggtctaa aaacttcata acatcctctt tctgtcagtt gaaaaataaa atttcatttg
    11761 ctcaatgaaa acttattgaa aattataaaa aaatatcggg tcggtttttt atccgcccca
    11821 agatgcgccg tctgaaacat ttcgggtgtg tggagaggtt tctgtttttt ccgataaatt
    11881 cctgcggctt ttcgcttccg gattcccgct tttgcgggaa tgacgaatta aagattatct
    11941 taaggtcaag ggactggatt cccgctttcg cgggaatgac ggcggcgggt tgctgttttt
    12001 tgtttataaa tttctgtaac tttgaatttc agccatcttc aataaattat cgaaacgtat
    12061 ca
//
LOCUS       DAWFIE010000063        12056 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWFIE010000063 DAWFIE010000000
VERSION     DAWFIE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12056
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.063"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            98..814
                     /locus_tag="WGQ71_001548"
     CDS             98..814
                     /locus_tag="WGQ71_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ0707534.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            963..1865
                     /locus_tag="WGQ71_001549"
     CDS             963..1865
                     /locus_tag="WGQ71_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ0707535.1"
                     /translation="MDSLMTLLSVLVPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSENAGMYCLMLLVLLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1908..2681
                     /locus_tag="WGQ71_001550"
     CDS             1908..2681
                     /locus_tag="WGQ71_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ0707536.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEAGSLKRHHGGASSGGNLPEGLPADRQIRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVVSELVRRRGSLRVITNNIHAASIASARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2793..4481
                     /locus_tag="WGQ71_001551"
     CDS             2793..4481
                     /locus_tag="WGQ71_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ0707537.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLIKDGKAYVDDLTLEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIHAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFEAGKTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4574..5530
                     /gene="gshB"
                     /locus_tag="WGQ71_001552"
     CDS             4574..5530
                     /gene="gshB"
                     /locus_tag="WGQ71_001552"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ0707538.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSSGLSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            5842..6225
                     /locus_tag="WGQ71_001553"
     CDS             5842..6225
                     /locus_tag="WGQ71_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ0707539.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            6340..6813
                     /locus_tag="WGQ71_001554"
     CDS             6340..6813
                     /locus_tag="WGQ71_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ0707540.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMSAMDHGHHHGEAHQH"
     gene            6939..8042
                     /gene="mnmA"
                     /locus_tag="WGQ71_001555"
     CDS             6939..8042
                     /gene="mnmA"
                     /locus_tag="WGQ71_001555"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ0707541.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGGGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            8113..9783
                     /locus_tag="WGQ71_001556"
     CDS             8113..9783
                     /locus_tag="WGQ71_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ0707542.1"
                     /translation="MKKIWLDSYEKGVSAEIDITQYGSVSDVFCQSVKKFAGQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPVALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIIASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETISFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITDTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDTRGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            9895..11529
                     /locus_tag="WGQ71_001557"
     CDS             9895..11529
                     /locus_tag="WGQ71_001557"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ0707543.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGVHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSGHIRERHRHRYEVNNNYVPQLEKAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            11544..11745
                     /locus_tag="WGQ71_001558"
                     /pseudo
     CDS             11544..11745
                     /locus_tag="WGQ71_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246312.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11756..12056
                     /locus_tag="WGQ71_001559"
                     /pseudo
     CDS             11756..12056
                     /locus_tag="WGQ71_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2980 a         3280 c         3170 g         2626 t
ORIGIN      
        1 aatttgtgca atgctttaat tcctctgaca cgatttgaag cacagccgcc ttaaaacatc
       61 ttaaaaaatt tgaaagcgta aaaatacgga aaacatcatg aactttgaaa acgacgacat
      121 tatccatgcg ccgaccacgt cttccctgat tctcgaagag cggcacgatt cggagctgtt
      181 ccgtgtttac gccctgattt tggacggcat taccgatcag gtgctgctgc ccggcaaaaa
      241 gctgaccgag tccgaacttt gccgccagat ggtgtgttcg cgcaacaccg tccgcggcgc
      301 gctgtcgctt ttggcgcacg acaagattgt cgatttgcaa cccaacaggg gcgcgttcgt
      361 ccacgttccc gatttgaaag aaatgcagga tgtgttcaat gcgcgcatcg aaatggagac
      421 gatgattttg aatatcctcg ccggcctgcc ggatttggaa acgcgcctca agccgcttta
      481 tgcgatgata aggcgcgaag aagaggcttc cggcaggggc gaccgcgtcg gctggaaccg
      541 cctgtccaat gccttccacg tcgaactggc gcgcctggtg ggcaacgatg tgctgttcga
      601 cattatgaac acgctgtgcg cgcggtcttc cctgattgtc gccgtggcgg gcgtgcatcg
      661 cgaggaaaaa cacgccatca atacgcatac gcattccgaa caccgcgaaa tcctcgacct
      721 gctgctggcg ggcaggcgca accgcgtggt caaaatcctg cgccgccatt tgggcaactg
      781 tatggaacgc ttggaaaaga ctttggaaga ttgaatgccg gagcggataa gccgcctgaa
      841 ccttcaggcg gttttttaat ggcgaacctg atgccgtctg aaatatggat gcgggtatct
      901 gcaattttca gacggcaatt tttaaaccgc atatatcatg cggcaataaa ggagggcagt
      961 agatggacag cctgatgaca ttgctttcgg tattggtacc gatgttcgcc ggatttttta
     1021 tccgtgtgcc caagccttac ctgcccgctt tggacaaggt gctgtcggtt ttggtgtatg
     1081 ccgtgctgct gctgatcggc gtatcgttgt cgcgcgtgga ggatttgggt tcgcggttgg
     1141 acgatatggc gttgacggtt ctgtggctgt ttgtttgtac ggtcggggcg aacctgcttg
     1201 ctttggcagt gttgggaaag ttattcccgt ggcggataaa ggggaaaggg aagggcgttt
     1261 cggtcggtgt gtcgggcagt gtggggcagc tcggatgcgt gctgctcgga tttgcatccg
     1321 gcaaactgat gcgcgatatt tggatgccgt ctgaaaacgc gggtatgtat tgtctgatgc
     1381 tgctggtgct cctcatcggc gtacagctca aaagcagcgg cgtatcgttg cggcaggttt
     1441 tggtcaaccg caggggtatt cggttgtcgg tctggtttat gctttcatct ctttcgggcg
     1501 ggctgctgtt tgccgcatcg gcagacggcg tgtcgtggac gaaaggtttg gcgatggctt
     1561 ccggcttcgg ttggtattcc ctctcgggtt tggtcatgac cgaggcttac ggcgcggtat
     1621 ggggcagcat catgctgctg aacgatttgg cacgagagct gtttgcactg gcatttatcc
     1681 cgctgctgat gaagcgtttt ccagatgcgg cggtgggggt tggcggtgcg accagtatgg
     1741 attttacatt gcccgtgatt cagggtgcgg gcggtttgga agtcgtgccg gtagcggtca
     1801 gcttcggcgt ggtggtcaat atcgccgccc cgtttctgat ggtggtgttt tccgctttgg
     1861 gttgaacgcg gtaaaatcgg catcccgatg caaggaggca gaaaacgatg aaaccgaaaa
     1921 tccaaaggca cggagagatt ttaagccttg tccgccggca tcagtttatg tcggtggacg
     1981 agcttgccgc cgcattggac gttaccccgc agacgatacg ccgcgacatc cgcgagttgg
     2041 aggaggccgg cagcctgaaa cgccatcacg gcggcgcatc ttcgggcgga aacttgccgg
     2101 agggcctgcc cgccgaccgc caaatccggt gtcaaaacga aaaaaacgcc attgctcggc
     2161 tgattgcgga acacatcccc gacggttcgt cgctgtttgt cagtatcggc acgacgatgg
     2221 aggccgtggt gtcggagctg gtgaggcggc gcggcagcct gagggtgatt accaacaata
     2281 tccacgccgc ctcgattgct tcggcgcgga cggattatac ggtcattatc acttccggag
     2341 tcgtccgtcc tttggacggc ggtattaccg gcgtggcaac cgtcgatttt atcaaccagt
     2401 tcaaagtcga ttatgccgtg atgagtacgc acggcgtgga aagcgacggt tcgcttttgg
     2461 acgacgacta caaagaagtc agcgtgatgc aggcgatgat tgccaacgcg cgcgtccgct
     2521 tcctcggcgt ggaccacagc aaattccgca gcaacgcgct ggtcaggctt ggcgacatta
     2581 cggcgtttga caaagtattt accgaccggc tgcctgatac cgcgatgcag aagatgttga
     2641 aagaggcggg ggtggaatgc ctgattgccg atgccgtctg aacgctatgt caaagcgcgc
     2701 aagtcgggta caataaacac atcatcaaac cgcttcagac ggcataagga accgccccaa
     2761 tgccgtctga agccatctgt tcaaagaaaa ctatgctcaa taaagaccaa tttgcggaca
     2821 accattttat ccgcaccatc atcgaagacg acctcaaaag cggcaaacac gaagccgtcc
     2881 aaacccgttt tccgccggag cccaacggct acctgcacat cggacacgcc aaatccatct
     2941 gcctgaactt cggtttggcg tatatttatg acggcttgtg caacctgcgt ttcgacgata
     3001 ccaatcctga aaaagaaaac gacgaatacg tcaacgccat caaagaagac gttgagtggt
     3061 tgggtttcca ttgggcaggt gagccgcgtt ttgcctccaa ctatttcgac cagctttatg
     3121 actacgccgt cggtttaatc aaagacggca aggcgtatgt cgatgatttg acgctcgaag
     3181 aaatgcgcga ataccgaggc acactgaccg aagcaggcaa aaacagccct taccgcgacc
     3241 gcagtatcga agaaaacctc gacctgttca cacgcatgaa aaacggcgag tttcccgacg
     3301 gcagcaaaac cctgcgcctg aaaatcgaca tggcatccgg caacatcaat atgcgcgacc
     3361 ccgtcatcta ccgcatccgc cgcgcccatc accacaacac cggcgacaaa tggtgtatct
     3421 acccgatgta cgactacacg cattgcatct ccgatgccat cgaaggcatc acgcattcct
     3481 tgtgtacgct cgaattcgaa gcacaccgtc cgctgtacga ctgggtgttg gataacatcc
     3541 acgcgccgca tgccacccgt ccgcgccaat acgagttttc ccgtttggag cttttgtaca
     3601 ccattacctc caaacggaaa ttgaatcagt tggttgtgga aaaacacgtt tccggctggg
     3661 acgatccgcg tatgccgacc atttccggta tgcgccgtcg cggctacacg cccgaaggcc
     3721 tgcgtctgtt tgccaaacgc gccggtattt ccaaatctga aaacatcgtc gatatgagcg
     3781 tgttggaagg tgcgattcgc gaagagctgg aaaactccgc cccgcgcctg atggcggtgt
     3841 tgaacccgct caaagtaacc ctgaccaact ttgaagccgg caaaacccaa agccgccgtg
     3901 ccgcgttcca tccgaaccac gaggaaatgg gcgagcgcga agtacctatt tcacaaacca
     3961 tctatattga agccgacgac tttgccgaaa atccgcctaa aggcttcaaa cgcctgattc
     4021 ccggcggcga agtgcgtttg cgccacggct atgtcatcaa gtgcgatgaa gtcgtcaaag
     4081 acgaagcagg caatgtggtt gaactcaagt gcagcatcga ccacgatacc ttgggcaaaa
     4141 atccagaagg ccgcaaagtt aaaggcgtga ttcactgggt gtctgccgaa cacgccgccg
     4201 aaatcaaagt ccgcctgtac gaccgcctct ttaccgtcga gcgtcccgat gccgtgcgcg
     4261 gcgaagatgg cgaatacctg ccgttcaccg atttcctcaa tccagaatcc gtcaaggaaa
     4321 tcaccgccta cgccgaacct gccgccaaag atttgccggc agaaagccgt tggcagttcg
     4381 agcgcatcgg ctacttcgta accgaccgca aagaccacgg caaagacacg ccggtgttta
     4441 accgcacggt cacgctgaaa gactcttggc agcctaagta aaactccatc cttgccgtct
     4501 gaatattgtt cggacggcat ttcccctttg cccgcgaaat gcggcacatt cggcacaccg
     4561 gaaaggaaat atgatgaaag tcctctttat cgccgacccg atggcaagtt tcaaaactta
     4621 caaagacacc acctacgcaa tgatgcggga aatggcaaaa cgcggctggc ggctgtttca
     4681 taccttgagc agcggattgt cggttaaaaa cggtctggtg gttgctgatg cgtctgcttt
     4741 tgagtttctc ggcgcacaag acgaccacga caaaaactgg tttgccgccg ccgacaaagt
     4801 tcagacggca ttgaaagcat ttgatgccgt gattatgcgt accgatccgc cgttcgatat
     4861 gcaatacctc tacgccaccc aattactgac gctggcggaa cagcagggcg cgaaagtctt
     4921 taacagcgga cagtcgatgc gtgacttcaa tgaaaaactg gcgattttga acttcagccg
     4981 ctttaccgcg cccacgctgg taacgacccg ttccgccgat gtccgcgcat ttttgaaaga
     5041 acacggcgac attatcatca aaccgctgga cggtatgggc ggtatgggta ttttccgcct
     5101 gaccgaaaaa gaccccaaca tcggcagcat cctcgaaacc ctgatgcggc ttgattcccg
     5161 caccattatg gcgcaacgct acattcccga aatcgtacac ggtgacaaac gcatcctgat
     5221 tatcggcggc gaagtcgtcc cctatgcttt ggcgcgtatc ccgcaaaacg gcgaaacacg
     5281 cggcaacctt gcggcaggcg gacgcggcgt ggcgcaggaa ttggacggac gcgaccgcga
     5341 aattgccgaa acgctcgccc ccgaactcaa acggcgcggc atcctgcttg ccggtttgga
     5401 cgttatcggc agcaacctga ccgaagtcaa cgtaaccagc ccgaccggat tccaagaaat
     5461 catgaaacaa aaaggtttcg atgtggcggc aatgtttgcc gatgccgttg ccgcgtggtc
     5521 ggtacgttaa accgatgtcg tctgaaaggc ttttgcttca caaccgtttg gtttgttcgg
     5581 gcaggcaggg ttttccctca gccggcagag atacgcttaa tgccgtacgg acacaccgcc
     5641 gccccgtttt cagacggcat atatagtgga ttaacaaaaa tcaggacaag gcggcgaagc
     5701 tgcagacagt acaaatagta cggaaccgat tcacttggtg cttcagcacc ttagagaatc
     5761 gttctctttg agctaaggcg aggcaacgcc gtacaggttt ttgttaatcc actatattga
     5821 gaacattttg aaaggatacc gatggaacct tcctcctacg cggcagaaaa aaaaggcaaa
     5881 ggcggcatca ggcgcgtcat taacgcattc ggctattcga tagacggcat cgccgccgcc
     5941 taccgttacg aagcggcatt ccgtcaggtt ttgtggctga acgtactgct ggtgtgcgcg
     6001 gcattttttt gggtttccga aaccgccgtc cgcctgccgt tgattatcgc gtcttttgtg
     6061 tcggtcattg tcgaactgtt caacaccgcc gtcgaagctg ccgtcgatca tacttcgacc
     6121 gaaaaacacg agctggccaa acgcgccaaa gacgcaggtt ctgccgcaca attgttcgcg
     6181 atgctgatgt tggcggcggt ttggctgtct gccctgttcg ggtaaaacgc ttgcagcagg
     6241 atttgtaatc ctttaggatt agtatgtggt atttattatt cacttaatcc actataagat
     6301 taagcacatt ccccgtctga tattataagg agcagaaaga tgaaaaaatt attggcggcc
     6361 gtgatgatgg caggtttggc aggcgcggtt tccgccgccg gagtccacgt tgaggacggc
     6421 tgggcgcgca ccaccgtcga aggtatgaaa ataggcggcg cgttcatgaa aatccacaac
     6481 gacgaagcca aacaagactt tttgctcggc ggaagcagcc ccgttgccga ccgcgtcgaa
     6541 gtgcataccc acatcaacga caacggcgtg atgcggatgc gcgaagtcaa aggcggcgtg
     6601 cctttggagg cgaaatccgt taccgaactc aagcccggca gctatcacgt gatgtttatg
     6661 ggtttgaaaa aacaattaaa agagggcgac aagattcccg ttaccctgaa atttaaaaac
     6721 gccaaagcgc aaaccgtcca actggaagtc aaaatcgcgc cgatgtcggc aatggaccac
     6781 ggtcatcacc acggcgaagc gcatcagcac taatctgctg aaaatatttg aaatgccgtc
     6841 tgaaaaagcc cgggctttca gacggcattt ttatgaccgc ctttaaaatg tgttaaaatc
     6901 tgcctttaaa aaccgccgtt tccaaccata ttctacgcat gaatacgaca gcaaaccctt
     6961 ccaatatcat cgtcgggctc tccggcggtg tcgattcttc cgtaaccgcc gccctgctca
     7021 agcagcaggg ttatcaagtg cgcggtgtgt tcatgcaaaa ctgggaagac gacgacaacg
     7081 acgaatattg cagcatcaaa caggattcgt tcgatgccat cgccgttgcc gatattatcg
     7141 gcatcgatat cgacatcgtt aatttcgccg cgcaatataa agacaaagtc tttgcttatt
     7201 ttcttcagga atacagtgcg gggcgcacgc cgaatcccga tgtgttgtgc aacgccgaaa
     7261 tcaaattcaa atgctttttg gattacgccg tagggcaggg cgcggatacc atcgccaccg
     7321 gacactatgc gcgcaaagaa gtccgcaacg gcgtgcatta cctgctcaaa ggtttggatc
     7381 gaaacaaaga ccaaagctat ttcctctacc gcctcaagcc tttccaactc gagcgcgcga
     7441 tttttccgtt gggcggtttg gaaaaacccg aagtccgccg ccttgctgcc gagttcaaac
     7501 tgccaaccgc cgccaaaaaa gacagcacgg gcatctgctt catcggcgaa cgcccgtttc
     7561 gcgagtttct gcagaaatac ctgccgaccg acaacggcaa aatggttacg cccgaaggga
     7621 aaaccgtcgg cgaacacgtc gggctgatgt tttacacatt gggtcagcgc aaaggtttgg
     7681 gcatcggcgg cgggggagaa ccgtggtttg ttgcggctaa agatttgacg aaaaacgaac
     7741 tgattgtcgt gcaaggacac gaccatccgc tgctctatac ccgcagcctc gtcatgaatg
     7801 atttgagttt cacgcttccc gaacgtccga aggcagggcg ttatacctgt aaaacgcgtt
     7861 accgcatggc ggacgcgcct tgtgagttgc gctatttgga tgaagaaacc gccgagctgg
     7921 tgtttgacga accgcaatgg gcggttacgc cgggccagtc tgccgtgctg tacgacggcg
     7981 acatctgttt gggcggcggt atcatccaaa cgaccgacaa gcccgtcatc atcacacgat
     8041 aaaggtaatg ccgtctgaaa cagttttcag acggcattgt tccgctcaat tccactttaa
     8101 agaccgatac ctatgaaaaa aatttggtta gacagctacg agaagggcgt cagtgccgaa
     8161 atcgacatca cgcaatacgg ttccgtcagc gatgtatttt gccaaagtgt caagaaattt
     8221 gccggccaac ctgcttttca aaatatgggc aaaacgctca cttatgccga aaccggcaaa
     8281 ctggcaacag attttgcttc ttatctgcaa aacgtcctga agctgccgcg cggcgaacgt
     8341 gttgccatta tgctgcccaa cgtattgcaa tacccggttg cgcttttcgg cattttgcag
     8401 gcaggtttgg tggcggtgaa caccaatccg ctctataccc cgcgcgagct ggagcatcag
     8461 ttgaaagaca gcggcgcgac cgccatcatc gttttggaaa atttcgccaa cacgctggag
     8521 ctggtgctgc cgcgcacgca gatcaaacac gttatcatcg cctctgtcgg cgaaatgttc
     8581 gggctgctta aaggttcgct gatcaatttc atcatccgga aaatcaagaa aatggtgccc
     8641 gaataccgta ttcgggaaac catttccttt cagacggcat tgaaagaggg ggcgaagcac
     8701 gttttccaac ctgtcgcatt aaaccgcgaa gataccgcgc tgttgcaata cacgggcggc
     8761 acgacaggtg ttgccaaagg ggcggtgctg agccacggca acatctgcgc caatatgctt
     8821 caggcaaaag aatggattaa aaaccagttg cgtgagggaa aagaaaccgt tatcgccgcc
     8881 ttgccgctgt accatatttt tgccttaacc gtgaatctga tgatttttgc caatgccggc
     8941 tcgaaaattg tcctgattac caacccgcgc gatatgaaag gctttatcgg cgaactgaaa
     9001 aaacagcggg ttaatgtatt tatcggcgta aacacgctgt ttaacgcgat ggttaaccgg
     9061 cccgattttg ccgaagtcga tttttcagga ttgcggctga ctttgggcgg cggtatggcg
     9121 acccaaaaag ccgttgccga aaaatggaaa aaaatcaccg acacgcccat cgtcgaagcc
     9181 tacggtctga ccgaagccag ccccggcgtg tgctgcaacc ccttaaacat cgaatcatac
     9241 agcggcagca tcggtttgcc cgtctcgtcc accgaagtcg aactgcgcga cgcaaacggc
     9301 aaagaagtcc ccgtcgggca gccgggcgaa ttgtgggtaa aaggccctca agtgatgcaa
     9361 ggctactgga accgccccga agaaaccgcc aaagccatag acacgcgcgg cttcttggaa
     9421 actggcgata ttgccgtcat ggacgaaaaa ggctggttga agctggtcga tcgtaagaaa
     9481 gacctcgtcg ttgtttccgg attcaatgtt tatccgaacg aaatcgagga agtcatcgcg
     9541 catcacggca aagtgatgga agtcgcctgt atcggcgtac ccgacgaaaa aaccggcgag
     9601 gcactcaaag tattcgtcgt caaaaaagac ccgtcattga ccaaagaaga acttatcgcc
     9661 ttttgccgca ccgaattaac cgcatataaa gttccgaaaa atatcgaatt ccgcgacgag
     9721 ttgcccaagt ccaacgtcgg caaaatcctg cgccgcgagt tgcgccaaag taccgggaaa
     9781 taaagaaaag atgccgtctg aaaacagccg tccaccgttc agacggcatc cgtccgtttg
     9841 ccagaaccgc gcgcttcacg ttaaaatcac gcattccaat atgggtattc catcatgacc
     9901 aaattcattt tcgtaaccgg cggcgttgtc tcctcactgg gtaaaggtat cgccgccgct
     9961 tctattgccg ccatcctcga atcgcgcggc ttgaacgtta ccatgctcaa gctcgatcct
    10021 tatatcaacg tcgatcccgg cacgatgagc ccgttccaac acggcgaagt gttcgtaacc
    10081 gacgacggcg cggaaaccga cctcgacttg ggacactacg aacgctttat cgattccacg
    10141 atgacccgcc gcaacagctt cagcacgggg caggtgtacg aaaacgtcat cgccaaagaa
    10201 cgacggggcg actacctcgg cggcacggtt caagtcatcc cgcacattac cgacgaaatc
    10261 aaacgccgca tccacgaagg cgcggcaggt tacgatgtgg cgattgtcga aatcggcggc
    10321 acggtcggcg acatcgaatc gctgccgttt ttggaagcca tccgccagat gcgaagccag
    10381 ttgggacgca acaacaccct gttcgcccac ttgagctacg ttccctacat cgccgctgca
    10441 ggcgaaatca aaaccaagcc gacccagcac accgttaaag aaatgttgag catcggtttg
    10501 caacccgaca tcctgatttg ccgtatggac aggacaatgc ctgcggacga acgccgcaaa
    10561 atcgccttgt tctgcaacgt ggaagaacgc gcgattgtcg gcagctacga tgtggacagc
    10621 atctacgaat gccccgaaat gctgcacgac caaggcatcg ataacatcat taccgagcag
    10681 ttgcagctta atgtgcagca ggcggatttg accgcgtgga aaaaaatcgt ccacgccatc
    10741 caaaacccga aacacaccgt caaaatcgcc atggtcggca aatacgttga tttgaccgaa
    10801 tcctacaaat cactgattga agccttgaaa cacgcaggtg ttcacaccga aaccgacgta
    10861 caaattacct ttgttgacag cgaaagcatc gagaaaaaca acggcgacgt ttctatgctc
    10921 aaagatatgg acgccatcct cgttcccggc ggttttggtt cgcgcggcgt ggaaggcaaa
    10981 atcgccgctg tgcgctatgc ccgcgaaaac aacgtgccat acttgggcat ctgcctcggt
    11041 atgcagattg cgctgatcga atacgcccgc gacgtggcag gtttgaaagg tgcgaattcc
    11101 accgagttcg acctcaaatg cgccgccccc gtcgttgccc tgattgacga atggcaaact
    11161 gccgacggca gcgtcgaaac ccgcgacgaa tccgccgatt tgggcggcac catgcgtttg
    11221 ggcgcgcaag aagtcgaatt gaaagcaggc agcctcgccg ccaaaatcta cggcagcgga
    11281 catatccgcg aacgccaccg ccaccgctac gaagtcaaca acaactatgt tcctcagttg
    11341 gaaaaagcag gcttggttat cggcggtgta tccgccggac gcgaacgctt ggttgaaacc
    11401 atcgagctgc cgaaccaccc ttggttcttc gcctgccagt tccaccccga gtttacctcc
    11461 aatccgcgca aagggcatcc tttgttcacc gcgtttgtca aagctgcgtt gaacaataaa
    11521 aaagcctgat aaagcgttac ttgatgatca aaatgccgtc tgaaagcctg aatgaagact
    11581 ttcagacggc attttcgcaa atcggggatt ataaaagcca tcaatttaat ttttatggtt
    11641 ttatctaata tttaccaact gaatattgtt aagcggggcg tgaaactcaa gttgagacct
    11701 ttgcaaaatt ccccaaaatc cctaaatttc caccaagcca tttaggggat tttccatgag
    11761 caccttcttc cggcaaaccg cccaagccat gattaccaaa cacatcgacc ggttcccgct
    11821 attgaagttg gactaggcga ttgattggca gccgattgag cagtacctga atcgtcaaaa
    11881 aacccgttac ctccgagacc accgcggccg tcccgcctac ccgctgttgt ccatgttcaa
    11941 agccgtcctg ctcggacaat ggcacagcct ctccgatccc gaactcgaac acagcctcat
    12001 cacccgcatc gacttcaacc tgttttgccg tttcgacgaa ctgagcatcc ctgatt
//
LOCUS       DAWFIE010000064        11370 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWFIE010000064 DAWFIE010000000
VERSION     DAWFIE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11370
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.064"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(239..754)
                     /locus_tag="WGQ71_001560"
                     /pseudo
     CDS             complement(239..754)
                     /locus_tag="WGQ71_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1762..2532)
                     /gene="xth"
                     /locus_tag="WGQ71_001561"
     CDS             complement(1762..2532)
                     /gene="xth"
                     /locus_tag="WGQ71_001561"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0707544.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(2586..2873)
                     /locus_tag="WGQ71_001562"
     CDS             complement(2586..2873)
                     /locus_tag="WGQ71_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0707545.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAVWC"
     gene            2975..3208
                     /locus_tag="WGQ71_001563"
     CDS             2975..3208
                     /locus_tag="WGQ71_001563"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707546.1"
                     /translation="MDDCTGLYVPFPTFFANTRKGGLSDGIKLPPLPCLTLLPNGIIR
                     IVQKMPYNRRTSCPADASVPSGKQNTCLKRCFF"
     gene            complement(3341..4222)
                     /gene="nadC"
                     /locus_tag="WGQ71_001564"
     CDS             complement(3341..4222)
                     /gene="nadC"
                     /locus_tag="WGQ71_001564"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ0707547.1"
                     /translation="MPSENTLFPLPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQIVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTLPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(4298..5236)
                     /locus_tag="WGQ71_001565"
     CDS             complement(4298..5236)
                     /locus_tag="WGQ71_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707548.1"
                     /translation="MDAYTEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            5543..6655
                     /gene="nadA"
                     /locus_tag="WGQ71_001566"
     CDS             5543..6655
                     /gene="nadA"
                     /locus_tag="WGQ71_001566"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ0707549.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETADRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            6707..8215
                     /gene="nadB"
                     /locus_tag="WGQ71_001567"
     CDS             6707..8215
                     /gene="nadB"
                     /locus_tag="WGQ71_001567"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ0707550.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEASAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NYDGLHLTREGGHTCRRIAHVADYTGEAVMQSLLAQIRRRPHIRVCERQMALDIQTES
                     GVACGLIVLDRRTQETYLIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARSSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAEIAKQTPDFVSLDISRQPEAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQAKTSPRPSENPSAEAGIFADDLQNTFSRPVLQTFNQRHLGILRSDTGLRR
                     AIAQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(8391..9056)
                     /gene="pgmB"
                     /locus_tag="WGQ71_001568"
     CDS             complement(8391..9056)
                     /gene="pgmB"
                     /locus_tag="WGQ71_001568"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ0707551.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYLGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSDTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(9069..11327)
                     /locus_tag="WGQ71_001569"
     CDS             complement(9069..11327)
                     /locus_tag="WGQ71_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ0707552.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGRQTDWQVSNSFEAEVGGTPETFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGNDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDESYLAKEGLE
                     VLVEVSRFWADRVHFSKRSGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVSAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPSAWTGYTFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLNGSHTAALEK"
BASE COUNT         2469 a         3054 c         3147 g         2700 t
ORIGIN      
        1 atccatccat acggaaacct gcaccacgtc attcctacga acctacattc cgtcattccc
       61 acgaaagtgg gaatccagaa cgtaaaatct gaagaaaccg ttttacccga taagtttccg
      121 tgccgaccgg tctggattcc cacctgcgcg ggaatgacgg ttcgggtatt tctgacgatt
      181 catcgagccg tagcaactgt atttttcacc ccgtcgggca aaaataccaa aactcaaatc
      241 aagccgtttg gataccgttt tcggcggtat cgttttcggc aaaataatca cgcatccggg
      301 tattcgatat cgtcagcagt ttgcgcatac atgccgtaac ggcaacctta tacggcttac
      361 ccttggacag caggcgttgg tagaaatccc gaataagcgg ttcaaaacgt gtcgctgcca
      421 cggtagccat atacagtgcc ttacgcaccg cagaccttcc gccaaagcag cggcttttga
      481 atttggtttc ctcgctctcc ctcgggtgcg gggcaatgcc ggccaaactc gctatccgtt
      541 tgtgcgacag ccgccccaat tcgggcagca tcgccatcag cgtagccgtc gttatcgaac
      601 cgatgccttt gatttgctcc gccacttggg ctttgccgtc aaaatgcgtg tgggtgtggt
      661 cgtcgatttg tttgtccaat tcgtcaatca gccggtcaaa atgggcaatc agttgtttga
      721 cgcttccgac ttgcgtttca tgaaactgat gcagatatag tggattaaca aaaatcagga
      781 caaggcgacg aagccgcaga cagtacaaat agtacggcaa cgctgtactg gtttaaattt
      841 aatccactat attttgtaat agctgccatt atgcctacta ggatatatac cttctatata
      901 atccttattt aaataaaaac ccttataaaa atctgtgtta tctgtcggga ttgaaaaaaa
      961 gattttatct ttctcatcag gtttatattt gctatcaata tcaaaaagag aataaggatt
     1021 acaatgagtt cgacatacta ggtaaaaatt tatatcttgg atcatgaaat attcgatatt
     1081 tatcaggatc gaaagatgct aaaaattcat caatatcggt actggatatt ttgtcatcac
     1141 taataaacat cgctagagat gggcgacttt gattttccat ttgttgatta gtaaaggata
     1201 taaatactaa cttgccattg tcaaaccaca tagatttata tagtggatta aatttaaacc
     1261 agtacgtcgt tgcctcgcct tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt
     1321 cctgattttt gttaatccac tatagttatt gcctttggga gaatcaattc tgacagcaca
     1381 taacataggt tcatctttac agtcgttata ttgggtttca tcaaactcaa ctgcatagca
     1441 caataaaggc aataggaaaa aaaggagatt aatttattac tatacataag gcagcctgat
     1501 tattcattct aaaataaatc cggtttttaa tatttgattc tgctccggat tatatacata
     1561 ataaatttta ctgctccaaa atctgatatt aaccctatgg atttcataaa tttttcagaa
     1621 aaataggctg tgcttgaaca atccgccgac tccggctgtt tgtcagggaa aaggcaatta
     1681 ttttcggtta gtcagcagcc tatcctattg aatcacttaa agatatggat gtttgtcaac
     1741 gccatatcaa aacacggtct tttaccaatc gaattctgcc gtcaccggcg cgtggtcgct
     1801 cggacgctcc agcgcgcgcg tctccaaatc gacgcggaca tccttcaacg ccgccgccat
     1861 cgcaggcgac accaaaatat ggtcgatacg caggcccagt ttgcgttgga acatcgcgcc
     1921 gcgatagtcg aaccatgtgt aaaacgcgcc ttcgggatgg acttggcgca ggctgtcggt
     1981 cagtcccaaa tccagcaggt tttgaaacca ctgccgttcg acggacgaac agtggatttt
     2041 ttcgtgccat ttttcagggt cgtaacagtc cgcatcggca ggcgcgatat tgaaatcgcc
     2101 cagtagcacc agtttgccgt agcgggtcat ttcatcgcgg acaaactccg tcagtgcggc
     2161 aaaccattgt tctttatatt tgaatttcgg gctgtcaagg gcttcgccgt tgacgcaata
     2221 gacattgatg acgcgcacac cgccgacggt tgccgcaatc acgcgccgtt gcggatcgtc
     2281 cggcagcgcg ggcagcccga tatgcacgtc ttccggcgcg ttgcggctga cgattgccac
     2341 gccgttgtag gttttctgcc cgctccaaac acagtgccag cccatcattt gcaaagcggc
     2401 ggccggaaat ttgtcctgat cgagtttgag ttcctgcaaa accaaaatat cgggcggatt
     2461 gtcggcaagc aggttttgca cctgcggcag ccgcacattg agcgaattga cgttccaagt
     2521 ggtgattttc ataacatttc cgaaaaatgc cgtctgaaac gttcagacgg caaaaggaaa
     2581 ggattttaac accataccgc gcgtttgttt tccaaatcgc ggattgtccg cagaatcgtt
     2641 gccgcgtctt cggaaatcag gcggtattcg ataatgcggt ggtaacgcgt aaagtccacc
     2701 agaccgcccg ccctcaggcg gttcaaatgg ccggaaacgg cggtggccgg cagggataag
     2761 gattttgcca gttcggcgat attgcgttcg ctgtccaaca gttggatcag tatcgccata
     2821 cgttcgggat gcgccataag tttgagtatg gtgtggagcg gtattgtgtt catcggtatt
     2881 cctaaaacct ttgccgcccg ccaagcggat tgacgggcga cagatatgga tgattgtgat
     2941 tatctttata aattgtatta taaatcaaat atttatggat gattgtacag gattgtatgt
     3001 tccttttccc actttttttg caaacacgcg taaaggcggg ctttcagacg gcataaaact
     3061 gccgccccta ccctgcctca ccctgttgcc gaacggaatc atccgcattg ttcaaaagat
     3121 gccttacaac cgccgcacgt cttgccctgc cgatgcctcc gtgccttcgg gcaaacaaaa
     3181 tacctgcctg aaacgctgtt ttttctaaag catcacgacc ttcacgaaaa atgccgtctg
     3241 aagaatattt cagacggcat ttcctccttg aggcaataac cggattacgg ttaggcggtt
     3301 ccttgtctga catatcagac aactgccgac accctaaaac tcatgccaag aaatccaaac
     3361 ctatgtccaa cgagcggctg ctgtgggtca gatagccgag ggcgatgccg tccacgccgg
     3421 tttgcgccac gcgcttcagg cggtcgaagc cgatgccgcc cgatgcttcg caatagacgg
     3481 tgtgggggtg ggcggtttgc gtgtggcagc ggtttgccgc ttctttcagg gtttcgtcgt
     3541 ccatgttgtc cagcaaaatc cgttccgcgc ccgctgcgat ggcttcgtcc agttgtgcca
     3601 acgtgtccac ttcgatttcc acgcaggtca atgctccgac cgcctgtttt gcctgccgaa
     3661 cggcttgggc gatgctgccg caataggcga ggtggttgtc tttgatgagt acggcatcgt
     3721 ccaaacccat gcggtggttc acgccgccgc ccgccctgac ggcgtatttt tgcaggacac
     3781 gcagcagggg gagggttttg cggctgcaca cgatgtctgt accgtattcg gcaacttcgg
     3841 caacggcacg cgcggtggcg gtggcgatgc cgcttaagtg cgtgaggtag ttgagcgcgg
     3901 tgcgttcggc ggcgagcagc gcgcgggcgt tgccttcgac ggcggccagc gtctgacctg
     3961 cgcggacgat ttgcccgtct cggatttcgg cttggaagcg gacggacgga tccatcgtct
     4021 gaaaggcgag gcgcgccaag tccatgccgg cgataacgcc gtcttcgcgg ctgacgagga
     4081 agagtttggc ggttttatcg ggcgcgatga cggcggcgga cgtaatatcg ccgcgcctgc
     4141 ccaaatcttc gctcaaggct tgttccacca tggggcgcaa tagggtgtcg ggcaggggaa
     4201 agagggtgtt ttcagacggc atggggtttc ctttgttgaa aaagatgggt gttgcaccga
     4261 tggggcaaaa cttcatttga tgccgtctga aaacgggcta acggctgccc agcggcaatc
     4321 cgatgtccga aatcagcctg tcgggcagga cgtcgtcgcg gaagcggcaa agctgcgcgg
     4381 gacggccttt gctgcccgat acgccggtat ccgacggctc gataaggttt tgctgctgaa
     4441 tctggcggcg gaagttttgc ttgtgcagca atcttccgct gatggcttcg acactgtttt
     4501 gcagttgcag cagcgtgaat tcgggcggca tcagttcaaa aatcacgggg cggtatttga
     4561 ttttggcgcg caggcgggac agggcggtcg ccagtacgcg acggtgatcg tggcgcatgg
     4621 gctgccccgt gagcgcgaag tcgaagtttg cctgcggctc ggcggcttcc actatcagac
     4681 cgctttcata cagcatttca tagcgttgta aaacgtattc ttccgaccag ttttccggtt
     4741 cgacccccca gcacaaatga atgcgcttga gccgcatttg acgcacttcc tccgtgtccg
     4801 ccgaatttgc ccaaatgcgc aggcggctga cgacggcgtc gcgctgcccg ccgtcggtgc
     4861 gcaagtcttc ccacgggaaa tagccgtagc agtcctgcca tttcgcatcc gggtgcagga
     4921 tgctgtcggc tgcctcgcgc accagcccca aatagctgac gtacaagacg ggcatgccgt
     4981 gttcgttgcg gcggtgggta tcgacaaagg tgtaaagctg ttccacatag cccataggct
     5041 gcgaagtctg cttggcaacc cacagcttca cgcccgcctg cagggaattg cgcagagggg
     5101 agagcgggcc gttgggcagg agtgtgcctt gggcgacggt taatacccgc aggccgccgt
     5161 cggtaacggc aatcaatacg ggaaccagct cgacgatgct ttgcggcggg gcttcggctt
     5221 cggtgtaggc atccatgctc ggctttctgc gggtgggaaa ggctttattg tatagtggat
     5281 taaatttaaa ccagtacagc gttgcctcgc cttgccgtac tatttgtact gtctgcggct
     5341 tcgtcgcctt gtcctgattt ttgttaatcc actataaacc aaagcggttt gtcggaacga
     5401 agcgggtcgg ttttacggta tcgcaaacct gttttcagac gaccttgctt attatactca
     5461 atttgagcat aatataaaaa tatttcaaaa tataaacggc aaatctttat gccgtctgaa
     5521 aaaccagcca taaggaagtc tcatgcaaac cgccgcccgc cgctcgttcg actacgatat
     5581 gccactcatc caaacgccga cttccgcctg ccaaatccgt caggcgtggg cgaaggttgc
     5641 cgacacgccc gaccgcgaga cggcagatcg tctgaaagac gaaatcaagg ctttgctgaa
     5701 ggagaaaaac gcggtcttgg tggcgcatta ttatgttgat ccgctgattc aggatttggc
     5761 tttggaaacg ggcggatgcg tgggcgattc tctggaaatg gcacgcttcg gcgcggaaca
     5821 cgaagccggt acgctggtgg tggcgggcgt gcgtttcatg ggagagagcg cgaaaatcct
     5881 ctgccctgaa aaaacggtgc tgatgcctga tttggaagcg gaatgttctt tggatttggg
     5941 ttgcccggaa gaggcgtttt cggcgttttg cgaccaacac cccgaccgca cggtggtggt
     6001 gtacgccaac acttccgccg ccgtgaaagc gcgtgccgat tgggtggtaa cgtcttcggt
     6061 ggcgttggaa atcgtgtcgt atctgaaatc gcgcggcgag aagctgattt gggggcccga
     6121 ccgccacctc ggcgactaca tccgccgcga aacgggcgcg gatatgctgt tgtggcaggg
     6181 ttcgtgcatc gtccacaacg aatttaaagg gcaggagctg gcggcgttga aggcggaaca
     6241 ccccgatgcg gtggtgctgg ttcatcccga atcgccacaa agcgtcatcg aactgggcga
     6301 cgtggtcggc tcgaccagca aactgctgaa agctgccgta tcacgccccg aaaaaaaatt
     6361 catcgtggcg accgatttgg gcatcctgca cgaaatgcaa aagcaggcgc ccgacaaaga
     6421 atttatcgcc gcgccgacgg cgggcaacgg cggaagttgc aaaagctgcg cgttctgccc
     6481 gtggatggcg atgaattcgc tgggcggcat caaatacgcc ctgacaagcg gacgtaacga
     6541 aatcctgttg gacaggaagc tgggcgaagc cgccaaactg cctttgcagc gtatgctcga
     6601 cttcgcggca ggactcaaac gcggggatgt gttcaacggc atggggcccg cctgatttgc
     6661 cgtccatatt gccgtttcag acgacctctc caacaaggac aacaccatgc aaaccgattg
     6721 cgacgtattg attgccggaa acgggctggc ggcactgacg ctcgccctgt cgctgcctga
     6781 atcgttccgc atcgtcattt tgtgcaaaaa ccggctggac gacaccgcca gccgtcatgc
     6841 gcaaggcggg attgcggcgg cgtggtcggg agaggacgac atcgaaaaac acgtcgccga
     6901 tactttggaa gcgagcgcag gtttgtgcga cgaagccgcc gtccgcacca tcctgtcgca
     6961 gggcaaaccg gcaatcgaat ggctgctggc gcagggcgtg gcgttcgacc gaaattatga
     7021 cggcctgcac ctgacgcgcg aaggcgggca cacctgccgc cgaatcgccc acgtcgccga
     7081 ctacacgggc gaagccgtca tgcagagcct gcttgcccaa atacgccgcc gcccgcacat
     7141 ccgcgtttgc gagcggcaga tggcgttgga cattcaaacc gaatcaggtg tggcgtgcgg
     7201 actgatcgtc cttgaccgcc gaacgcaaga aacctacctc atccgcgccc gccataccgt
     7261 actcgcaggc ggcggcttgg gacagattta cgccgccacc accacgccgc ccgaatgcac
     7321 gggcgacgcc atcgccatgg cgatacgcgc aggctgtgca gttgagaacc tcgaatttat
     7381 ccaattccac cccacaggct tggcaagatc gtctgaaaac ggccgtacct tcctgatttc
     7441 cgaagccgtg cgcggcgaag gcggcatcct gaccaaccaa gcgggcgaac ggtttatgcc
     7501 gcattacgac cgccgcgccg aactcgcgcc gcgcgacatc gttgcccgcg ccatcgccgc
     7561 cgaaatcgcc aaacaaactc cagacttcgt ctcgctcgac atcagccgcc aacccgaagc
     7621 gttcgtccgc cggcattttc cgtccatcca tcggcactgt ttatcccaat gcggcctgga
     7681 catcacgcgc caagccatcc ccgtccgccc cgtgcaacac tacacctgcg gcggcatcca
     7741 aaccgacccc tgcggcagaa cctccctgcc gcagctctac gccttaggcg aaaccgcctg
     7801 caccggcttg cacggagcca accgcctcgc cagcaactcc ctgctcgaat gtgtcgttac
     7861 cgccagactt gccgcccaaa ccatcgcgga cggacaagca ttccaagcca aaacgtctcc
     7921 aaggccgtct gaaaacccct ccgccgaagc aggcatcttt gcagacgacc ttcaaaacac
     7981 attcagccgc cccgtcctgc aaacgttcaa ccaacgccat ctcggcatcc tacgcagcga
     8041 taccggcctg cgccgcgcca tcgcccaact gcggctttgg aagcaaaacc aagccgaacc
     8101 gcacaccgcg tccgaatacg aaaaccgcaa cctgctcgaa tgcagcctcg ccgtcgccca
     8161 agccgcatac aggcggagac agaacatcgg cgcgcatttt aatagtgatt gttaaggttg
     8221 gctggttaga aaaaatcgtt caaatgaatt ttaatagcat ggttaagaga ttggcattca
     8281 gtttttaggg ttgcgtaagt tgggttatca atctgttcta aaagcagaaa ggccgtctga
     8341 aacactgttc agacggcctt ttacttcctt gacactttat ctgacgcgtt ttacctgccc
     8401 gactgtgccc acacgttttg caggtaggcg taggtcagct cggcggtatc ggacaccagc
     8461 gcgatgtcgc tgcccaagtc ttcggctttg cccacgccga taggcaaggc gccggcggct
     8521 ttgatggcgg cgacacctgc ggcagcgtct tcaatgccga tgcagcggcg gatgtccgcg
     8581 cccacgccct cggcggcggc gaggaagatg tcgggggcgg gtttggaatg cgcgacggcg
     8641 gcagggtcgg cgacggtgtc gaagaagtgg gtcagcccca tgcgttcgag caggaagggg
     8701 ccgtttttac tggcggacgc aagggcgatt tttttgccgt ttgcttttaa tgcttccagc
     8761 agcggcaaaa tgccgagata cacgtcttcg ggtttgactg cctgaatcat ctcgacgtag
     8821 ttgtcgtttt tgcggcgggt cagttcggcg aactcggctt cgccgacggt tttgccgccg
     8881 tgtgcgagga tgcgtttgag cgaatcgtcg cgcgacacgc ctttgagctg ctcgttaaac
     8941 ttgcggtcta tgccgatgcc cagttcttcg gcgagctttt tccatgcgcg gtagtggtat
     9001 tcggcggtgt cggtgatgac gccgtcgaga tcgaagagca ctgcggtaaa cgtcattttg
     9061 cgccctcctt atttttccaa cgcggcggta tggctgccgt tgagtgtgat gtctttgccg
     9121 tacacctgca aatcgagcgg ctcgcctttg agcagggtga agacgacgtt ttctttgccg
     9181 acggcgactt taatcagacg gcctcggtag ttgatatgga aggtgtagcc tgtccacgcg
     9241 ctcggcagga acggtgcgaa gctgagttta ccgccccaag ttttcatttg ggcgaaacct
     9301 tggacgatgg cgagccacga gccggtcata gaagtaatgt gcaggccgtc ttcggtgtcg
     9361 ttgttgtagt tgtccaagtc caggcgggct gtgcgccggt acatttccac ggctttttct
     9421 tctttgccca gttcggcggc gagaatagag tggatacacg gcgacaacga gctttcgtgc
     9481 acggtcatcg gttcgtagaa gtcgaaattg cggcgttttt cgtcgatatt gaaacggtcg
     9541 ccgaagaaat agatgccttg caagacatcc gcctgtttga tgaagggcga acgcaggatt
     9601 ttgtcccacg accatttctg gttgagcggc aaatcgtcgg gcgaaagcgc ggacacgggg
     9661 cggatgtctt tgtcgaggaa tccgtcgtgt tgtacgaata cgccgagttc ttcgtcatga
     9721 ggacggtaca tattcgcgct gatgtccgcc catttttcca actcggcggc gctcacgttc
     9781 aaatccggac gcgggtattt cgccagggct tcgcgggtgt agtccaatac ccatgcggcg
     9841 agggtgttgg tgtaccagtt gttgttgatg ttgttttcgt attcgttcgg gccggttacg
     9901 ccgtgaatca tgtatttgcc gctgcgtttg gagaagtgga cgcggtccgc ccagaagcgg
     9961 gacacttcga ccaagacttc caagccttct ttggcaagat agctttcgtc gccggtgtag
    10021 ttggtgtagt tgtagatggc gtaaggaatc gcgccattgc ggtggatttc ctcgaaggtg
    10081 atttcccatt cgttgtggca ctcgatgccg gtaaacgtta ccatcggata gagtgcgccc
    10141 gccaagccct gttcgcgcgc attgtgctgc gcctgcggca gttggttgcg gcggtattgc
    10201 agcaggttgc gggtaacttc gggttccgcc agcgcgaggt agagcggtac ggcgtaggct
    10261 tcagtatccc aataggttgc gccgccgtat ttttctccgg taaagccttt cgggccgatg
    10321 ttcaggcgcg cgtcttcgcc gtagtaggtg gagaacagtt ggaacagatt gaaacggatg
    10381 ccttgctgcg cttcgtcgtt gccttcgatg accacgtcgg cgatttccca acggtgcagc
    10441 cagcctgctt tgtgcgcgtc cagcaaggtt tcaaacgcaa cgcctgcgac tttttccgac
    10501 aaagcgcggc ctgcggcttt cactgcttcc aagccctgat aatcgcggct ggtggtaaca
    10561 ataacccgtt tttcaaaggt ttcgggtgtg ccgccgactt cggcttcaaa agaattggag
    10621 acctgccagt cggtttgtct gccgccgagg gctttaaagc tgccggcgaa ggtttgctcg
    10681 gcgttgacga taaattgttc cacgccgaaa gggttggcaa cggtttgggc ggcaatgtag
    10741 gagagactgt ctgaaacgcc tttgtccaat acctgccaga atttttcttc gtagttggag
    10801 tcttcgtttt tcacgtcggc atcgatgatg gaatcgatgc ggacttggtg ggttttgccg
    10861 tcaacggata cggcttccca gcggataacc gccagctctt tttgcgcgac ggacaggaat
    10921 ttgcacacat tgaaacgcac accgaatacg gtgaacgagc ggcgcaacac gccgtgctgc
    10981 atatcaagtt cgacggagaa gccggcaaca tcgtttttcg ccaagtccac ttcctgcccg
    11041 tcgacaaaga ttttgacttt gctgaaattg agcgcgttga tggctttacc gaaatatttg
    11101 gggtagccgt ttttccacca gccgacgcgg gttttgtcgg gaaaccacac accggcgatg
    11161 taggtaccca agtggctgtc ggcggaatag gtttcctcaa agctgccgcg catacccata
    11221 tagccgttgc ccaaactggt caggctctct tgcagccgtt tgtgttcttt ttccagcttt
    11281 gccgaacgca gcgtccaagg gctgatttcc ataattcttg tgtacattta tgaagctcct
    11341 gtttggattg atttgaggga atggtgaaat
//
LOCUS       DAWFIE010000065        10766 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWFIE010000065 DAWFIE010000000
VERSION     DAWFIE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10766
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.065"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(51..245)
                     /locus_tag="WGQ71_001570"
     CDS             complement(51..245)
                     /locus_tag="WGQ71_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ0707553.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(235..2316)
                     /locus_tag="WGQ71_001571"
     CDS             complement(235..2316)
                     /locus_tag="WGQ71_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ0707554.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANHVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            2792..3172
                     /locus_tag="WGQ71_001572"
     CDS             2792..3172
                     /locus_tag="WGQ71_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ0707555.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQVGTDDF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3254..3441)
                     /locus_tag="WGQ71_001573"
                     /pseudo
     CDS             complement(3254..3441)
                     /locus_tag="WGQ71_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222085.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3983..5416
                     /locus_tag="WGQ71_001574"
     CDS             3983..5416
                     /locus_tag="WGQ71_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ0707556.1"
                     /translation="MNEYSQLIKHPDISLSPISDGIGVGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALAVLAYEAGVPQDLLPVVSGSASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDAFCRKLSEKAAVLKLGNGLDEGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIAVANDTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEVAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCIDVG"
     gene            5660..6448
                     /locus_tag="WGQ71_001575"
     CDS             5660..6448
                     /locus_tag="WGQ71_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ0707557.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVIEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            6816..8372
                     /locus_tag="WGQ71_001576"
     CDS             6816..8372
                     /locus_tag="WGQ71_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0707558.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLSQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8389..9135
                     /gene="surE"
                     /locus_tag="WGQ71_001577"
     CDS             8389..9135
                     /gene="surE"
                     /locus_tag="WGQ71_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ0707559.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
     gene            9391..10638
                     /locus_tag="WGQ71_001578"
     CDS             9391..10638
                     /locus_tag="WGQ71_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0707560.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPASDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYIPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKAAAVK
                     SRPAVPAAAQPPVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNML
                     SIGQIVKVKPAGYAAPKTAAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKA
                     VPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAAD
                     GKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDAS
                     RTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT         2503 a         2764 c         3042 g         2457 t
ORIGIN      
        1 taaaaattcc gtctgaagcg gcgttcagac ggaatttgtt tcaagcaggc ttaacagcag
       61 cgtccgccgt ttgcgccgca gcggcgtttg cggcaatagt cttgaaactg cagcttggtc
      121 atcacagggg cgttgggatt atgtttgcgt tgttgcgcaa cgtagttttc ataatcgggc
      181 acacctgcca tcaagtttgc cgtcagcttg atggttttcc accaagacgc gagcttatgc
      241 ttcactttgt gcctccggct gtttgccgtc gcggtacacc gccgggattt ctttggcggt
      301 cggccagccg actttgcgtg ctttgagggc ggtacgcaaa ccgtacgcgg cgacaatcac
      361 gacaaccgac aagaagagga tggtcagacc ggcattaatc ttgtcgttga agatgatttg
      421 cgccatttcg ccgatgtctt tagcgggcgc gaggacttcg tttttagcca atgcgtcgct
      481 gtatttgccg gcgtgggcaa ggaagctgat gcgcgggtcg ctgtggaaca gtttttgcag
      541 gccggcgtag caggttacaa acagtacgcc gacggcggga acgagtacca cccagacata
      601 acggtcgcgt ttcatcttaa tcagcaccac ggcgcacata atcaaggcta cgcctgccag
      661 catttggttg gcgatgccga acaaaggcca gagcgagttg atgccgccca gcgggtcggt
      721 tacgccggtg tagaggaagt agccccacaa tgccacggcg aagaaggtcg caatcaggtt
      781 ggcagggatg gagtcggtgt tgccgaaagg tttgtagaag atgctgccta agtcttgaat
      841 cataaaacgt gcgacgcgcg taccggcatc gacggcggtc aggatgaaca aggcttcaaa
      901 caacagggcg aagtgatacc agaacgccat catcgcctcg cccggaatca ggcggctcat
      961 aatgtgcgcc ataccgactg cgagggtggg cgcaccgccg gcacgggaaa ggatggtgtt
     1021 ttcgcccact tccttagcgg tgtgcaacag ggttgccgca tcgacaggga attgcagctt
     1081 ggtggtaatc acttcggcgg cggtattggc atccgtaccg atcagggcgg cagggctgtt
     1141 catggcgaag tacacgccgg gatcaagcga tgcggcggcg gcaagcgcca taatggctac
     1201 gaaactttcc atcaacatac cgccgtaacc aatcatacgg acgtgggttt cgttttccag
     1261 cattttcggc gtggtgccgg aggaaatcag cgcgtggaag cccgaaaccg caccgcaggc
     1321 gatggtgatg aagaggaacg ggaacaatgc gcctgagaat accggacccg aaccgtcgat
     1381 aaagtgggtt acggcaggca tttgcaaagc gggattgacg atgacgatgc ccaaagccaa
     1441 ggccgcaatc gtaccgattt tcaggaaggt ggagagatag tcgcgcggag tgagcagcaa
     1501 ccatacgggc aatacggagg cgacaaagcc gtaaatcata atcgcccaag tgagctggat
     1561 gccgtcaagg tcgaaccaat gtccgatgga acttttcgcc acatcttcgc cgtaaattac
     1621 cgccagcatc agcaaaataa agccgacgat ggaaatctcg ccgattttgc ccggacggat
     1681 atagcgcgtg taaataccca taaacagggc aatcggcata gtggcggcga tggtaaacgt
     1741 accccaaggg ctgtgaacca gtgcttttac gacaatcaac gccaacaccg ccataatgat
     1801 gaccataatc atcaaaatac cgatggaggc aatcacgccg gggacagtgc cgagttcctg
     1861 tttcacaata tcgcccaaag acttaccgtc gcggcgcata gagacgaaca agaccatcat
     1921 atcctgtacc gcgccggcaa acaccacgcc gaagataatc cacaaagtac cgggcaggta
     1981 acccatttgc gccgccaaaa ccgggccgac caaagggccc gcgcccgcaa tcgcggcaaa
     2041 gtggtgtccg aacaatacgc ccttgtgcgt cggaacgtag tccaagccgt cgttgtggcg
     2101 ttctgccgga gtcaggcggt taggatcgag ccgcattacg tggttggcga tgtagaggct
     2161 gtaaaaacgg taggcgatgc agtaaacgga tatggcggcg gtaaccatcc ataccgcgct
     2221 gacctgttcg cctcggctga gagccagagt ggtaaaggat gctaagccga ccagtaccac
     2281 tatgccccaa atgaggaagg ttttgagtga tttcatcgaa taaatcctta tctcacactg
     2341 tcggaatatg cctgaacggc gggaaacgga tgaccgcctg ccggcacggg cacaaaccgg
     2401 agtctgaatg attgacgaag tatgaaacag tgcgcttgtc gggatttgtg ccttatgccc
     2461 gcatcaaaca gtgcagggat gcgggcgcac aaaacgttaa acgccgaata ggattttaac
     2521 gcaaattagc acactgatag cggtttttac ttggaatttg gaaaaaattt acattccttc
     2581 gggcggacag gcgggtttgg acggcatcgt caggcaaaag gcggcgccgg aagacgggta
     2641 aagaaggggc gcgcaatccg gattattgat tcatcgcggg taaattccgg ttatcgggct
     2701 tgtgtgtttg cgcgtccgtt tatagtatgg cgttgccgca gcttggaatc agggcggttg
     2761 tttcatatct tatttttatt ggggagcttt tatgaatatc aggtttttcg cgctgaccgt
     2821 accggttttg tctttggcgg cctgtgccgt gccggaggcg tatgatgacg gcggacgagg
     2881 gcatatgccg cccgttcaaa accaagtcgg cacggacgat tttcgggcgt tttcctgcga
     2941 gaacggtttg tctgtgcgcg tccgccattt ggacagcggc aaagtcgcgt tgcggctaga
     3001 cggcaggcgt gccgtcctct cttccgacgt tgccgcatcc ggagaacgct ataccgccga
     3061 acacggtttg ttcggaaacg caaccgagtg gcaccagaaa ggcggcgaag cctttttcgg
     3121 ctttaccgat gcctacggca attcggtcga aacttcctgc cgcgcccgtt aaacggggca
     3181 gcctgtttga accgccgtcc ggatttttcg ggcggttttt tgtgtcggac ggacggtttt
     3241 ttgtgtcgga tttgttttgc agttcggcct ccggcagggt tcgggctgcc tgataccgtc
     3301 cgtaacttcg ggtattgccg agttcgtcgg caacggccgc tgcggcttgg gcggcgtttt
     3361 gccgaacgcc tgcgttactt ccctttgtag gtcgatctct ttggcaaccg cgtctttgtc
     3421 aaagctgttt ttcagacgac cttttatcaa atattatcgg cagcggctca atgccaactt
     3481 taaacctgct ccgatttctt cagggctgtt atccaatgat aaaatatagt ggattaacaa
     3541 aaatcaggac aaggcgacga agccgcagac agtacagata gtacggaacc gattcacttg
     3601 gtgcttcagc accttagaga atcgttctct ttgagctaag gcgaggcaac gctgtactgg
     3661 tttttgttaa tccactataa tgtttggatt tttgccatac tctaccacac gttgcaactg
     3721 caatctttgc tctttatata ttgcacccca tcaaagggct gcattacttt tctttctaaa
     3781 gattactatc atataaaaaa tattttttaa acgacaggga ttgcgcccgc gccatattca
     3841 aacactgccc ggatgttgcg ctgcgcgatc ggatgcttca gatggcatcg gaagggtttg
     3901 cagttttgga atatgaagat gataatgtcc gcgagattga cggcatttga aagcggttat
     3961 cccgcatacc ggagggcacg aaatgaacga atattcccaa ttaatcaagc atcccgatat
     4021 ttccctttcc ccgatttcag acggcatcgg ggtcggcaat ccggcgacgg gcgaaatttt
     4081 ggcgtatgtc cgcaacacgg gttcggacaa actgaaaaac ctgattcaaa aggcggcggc
     4141 agcgcaaaaa ttatgggcgg cgaaaactgc tttggaacgc gcagacatat tgtggcgttg
     4201 gtatttttta gttaaagaaa acaaagaagc actggcgcgc ctgatgacga tggagcaggg
     4261 caaaagcctg accgagtcgc gcggcgaaat cgattatgcg gcttcgtttg tgcgctggtt
     4321 tgcggaagag gcgcggcgga ttgacggcga tgtgctgacg agtgtgaaag cgtcgcaaaa
     4381 actggtcgtg ttgaaacagc ctgtcggtgt taccgccgcg attacgccgt ggaactttcc
     4441 gtccgcgatg attgcgcgca aggctgcgcc agctttggca gtgggttgcg cgatgatcgt
     4501 caaacccgcg tcgctcacgc ctttgagcgc gtatgcgttg gccgtgttgg cttacgaagc
     4561 gggcgtgccg caggatttgc tgcctgttgt cagcggcagt gcttcggaaa tcggccgtga
     4621 atttgccacg aacccgatta tccgcaaaat cagcttcacc ggttcgaccg aagtcggcgc
     4681 gaaaattttt gccgacagcg cggcggacat taaaaaactc agtttggaac tgggcggcaa
     4741 tgcgccgttt atcgtgtttg acgatgccga tttggacaaa gccgtcgaag gcgcgctcgc
     4801 cagcaagttc cgcaacagcg gtcagacctg tgtctgcacc aaccgcgttt acgctcaatc
     4861 gggcatttac gacgcatttt gccgcaaatt aagtgaaaaa gcagccgtgc tcaaattggg
     4921 caacggcttg gacgaaggcg tgaaccaagg gccgctgatt gaggaaaaag cggtagaaaa
     4981 agtcgagcag cacatcgccg acgcactctc aaaaggcgcg gtctgtctga ccggcggcaa
     5041 acgcagcgcg ttgggcggaa cgttttttga gccgactgtc ttaagcggcg taacagcaca
     5101 aatggcggtg gcgcgcgagg aaaccttcgg gccgttgtgt ccagtgttcc gttttgaaac
     5161 cgaagccgaa gtcatcgcgg tcgccaacga tacggaatac ggtttggcag cttacctttt
     5221 caccgccgac accgcccgcc aatggcgcgt cggcgaagca ttggaatacg gtatggtcgg
     5281 catcaatacg ggcttaatca gtaatgaagt ggcaccgttc ggcggcgtga aacgcagcgg
     5341 tttgggacgt gaaggcagca aatatggtgc ggacgaatat ctagaattga aatatttgtg
     5401 tattgatgtc gggtagagta tatatttgat ttatatgcaa aaagtttttt ggtatgtaaa
     5461 tctgatagat tcacataccc caacttatac cccaatttat ttgaagttgg tttgaagcca
     5521 gtttgctacg gcttcttcgt tccaccggct gctcctgccg tctgaacggc agggtttcag
     5581 acggcgccgc attttaagca gtctctatct gttgtacaat gcgccctgtt tttacggtta
     5641 tttttgattt gaacaagata tgatggaaaa cggaaaaaca gtcccgagat ggcgttttgc
     5701 cttgaaaagt gcgggctggc acctcttaat cagcctgtcg gttgcagggc tggcggcatt
     5761 gctggttttt aaggtttggt atccttatcc ttatgccgag ctgacgggag ggctgtcgct
     5821 ttatcagctg gtggtggctg tcgatattgt atgtggtccg ctgctgactt taattttggc
     5881 aagcccgaag aaaaagacaa aggcacgcat ggtcgatttt tccatggtcg gcatcatcca
     5941 gctggcggct ttggtgtacg gtctgcacag cgtttctttg gcgcgccctg tgatagaggc
     6001 gtttgaaaaa gacaggatta atgttgtgac cgccgccgaa gtggcggttg aagatttgca
     6061 caaagcgccg gaagggctgc aaagcctgtc gtggttcggc atccgccgca ttgcattgaa
     6121 agaacctgag gatgcggatg agaagaacaa gacgctggat ttgtccctga aaggtatcga
     6181 gccgagtatg cgtcccgacc ggtggctgcc gtattccgac aaggaagcag aagaaatccg
     6241 caaacatctg aaaccgctga aagtcttggc ggatgcgaga aaaacgacgg ttgcggacat
     6301 tctgaaacag gcaggtctcg ccgaagggga ggagctgtat tacctgccgt ttaccagcag
     6361 caggcagaaa gagtggatag tcattaccga taaagagggc aacaccaaag gctacgcgcc
     6421 gatagacggc ttcatcatca ccccttaagc gttgggactc cgtccgcact cgaacatccg
     6481 ttcttcgcgg cggtagaatc agactgtatt tgagaggggc gcaacccctc aatcagggca
     6541 acacataggg gaaggcttta tgtgccgtcc gtgtgttgaa acatttcaaa ataagctcat
     6601 gccgtccgaa catcctttca gacggtatgg cgttttgcca ttgccccgat aagctgttaa
     6661 actatttaaa ttatttcgac cgaaggtaca cgcacatgca agaccccgag cagagcagca
     6721 agcccgcccg ccgttttttg tgcgtaccgt ctgttcaggc agcttgagcg cttcaggctg
     6781 ccttttcgcg tttgaacttg aggaatacga aaatcatgga tttcagttgg ttggcagaac
     6841 cgcatacctg gataggtttt gccacgcttt tggtgttgga agtcgtattg gggatagaca
     6901 accttgtctt tgtggcgatt ttggcaaaca aggtccagcc cgcacggcgc gaccgcgcac
     6961 ggattatcgg gctggggctg gcagtcgtca tccgcatcat tatgcttgct tttatggcgc
     7021 acatcatcac gctgaccgag ccgctgttcc aaatcggcgg cctcgccgtt tccggcaagg
     7081 acatgattat gctcgcgggc ggtattttcc tgctttacaa agccaccacc gaactgcatg
     7141 aacgcctcga agggcacaac cgttttaccg ttgccgacaa ccaaaaaaaa cacgcgccgt
     7201 tttggggcgt ggtcgcgcaa atcctgatac tggatgccgt gttttccatc gattcggtca
     7261 ttactgcggt ggcgatggtc gatcatatcg tcgtggcgat gggtgcggtc gtcgtcgcga
     7321 tggctgtaat gatttctgcc agcaaactct tgaccgaatt tgtcgacaga caccctaccg
     7381 tcgtgatgct ctgccttggt tttttgttga tgatcggttt cagcctgatt gccgaagcct
     7441 tccatttcca cattcccaaa ggctacctct acgccgccat cggcttctcg attttaatcg
     7501 aactgtttaa ccagatttcc cagcgcaaca gccgcaaaaa cgactacatc ggcagctcgt
     7561 ggcgcaagcg caccgccgaa aacgttttgg gtatgatggg catacgcgaa agcgtgcttg
     7621 ccgatgcggg cggcgaatcc ggggacgacg cgcattttga agaaaacgaa aaatcgatga
     7681 tacgcagcgt gctgacgctt gccgaacgcc cgattatggg ggtgatgatc ccacgccgcg
     7741 acatcgaacg gctggacatt tcccaaagcc gcgaagaaca gtgtgcccaa ctgcaaaaca
     7801 cgccttacag ccgcctgctc gttgtcggta aggcgggcgt ggacgaacct ttgggctaca
     7861 tcaacaaaaa agacctgctg tcccaactgc tggaaacagg cggtctcgac attcagacgg
     7921 cattgcgcca gccactcgtc ctgcccgaca gcaccaccgc gctgggcgca atcgaactct
     7981 tccgccaaag cagcgcggat tatgctttgg tggtggacga gttcggcgcg gtattgggca
     8041 tggtaaccat gaaagacctg ctcgaaacca tcgcaggcga gttccccgaa gaatttgagc
     8101 gcgaagaaga accagccgtt caggggaatc ccgatgaaag cctgacggtg gaaggcgcgt
     8161 tggaatatgt ggagctggca tcccaactca atctgtcgca gcaggaggaa gatgccgatt
     8221 tccatacggt tgccgggctg attatggaag aattgcaaac catccccgat gtcggcgatt
     8281 ttgccgattt ccacggctgg cggtttgaag tggtcgaaaa agaagggcag cgcatcgagc
     8341 gggtcaaaat caccaaattg cccgaagaat aagcattcag gatagaaaat gaacgttttg
     8401 atttccaacg acgacggcta cctctccgaa ggtattgccg ttttggcgcg cgttacggcg
     8461 gaatttgcca acgtcagggt ggttgcgccc gaacgcgaca ggagcggggt cagcaattcg
     8521 ctgacgctgg aacgcccttt gcagttgaaa caggcgcaaa acgggttcta ctatgtcaac
     8581 ggcacgccga ccgactgtat ccacatcggg cagtctgtat tttcggattt tcaggccgat
     8641 tttgtctttt cgggcatcaa ccggggcgcg aatatggggg acgacacgct ttattcgggg
     8701 acggttgcgg cggcaaccga agcctacctt atgggcatac ccgctgtggc gttttcctta
     8761 aacgacgctt ccggacgcta ttgggcgacc gcagaacagg cactgtggac attgttggcg
     8821 cattttttca aaaacccccc gcagtcccct attttgtgga acatcaatat ccccgccgtt
     8881 gcgccggaag atgtgcgggg cattaaaatc gcccgtttgg gcaggaggca tcacggtcag
     8941 aacgtcattc ccgcgcgcaa tccgcgcggc gaacagattt attggatagg accggtcggc
     9001 gaagtttccg atcgggaaga gggaacggat ttcggtgaat gcggcgcagg tttcattacc
     9061 gtaacgccgc tgcaaatcga cctgaccgcc tatccggaca tggcggaaac agcggcgttc
     9121 tggcatgcgg actgaccgtt tcatcaaata tagtggatta acaaaaacca gtacggcgtt
     9181 gcctcgcctt agctcaaaga gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt
     9241 tccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact
     9301 ataccgtcgg gctttgcgct tgagaagaac gcgcattttt attatgatat accgcattca
     9361 ggctatatat cggaacatga aggttatctt atgttgaaac aaacgacact tttggcggct
     9421 tgcgcctcct ttgccgcact gttgggcggt tgtgctaccc agcagcctgc ccccgtcatt
     9481 gcaggcaatt caggtatgca ggacgcgccg tcttcggcag tttacaacaa cccctatgga
     9541 gcaacgccgt acagcccggc tcctgccagc gatgcgcctt atgtgccgcc ggtgcaaagc
     9601 gcgccggttt atacgcctcc tgcttatgtt ccgccgtctg cacctgccgt ttcgggtaca
     9661 tacattcctt cttacgcacc cgtcgacatc aacgcggcga cgcatactat tgtgcgcggc
     9721 gacacggtgt acaacatttc caaacgctac catatctctc aagacgattt ccgtgcgtgg
     9781 aacggcatga ccgacaatac gttgagcatc ggtcagattg ttaaagtcaa accggcagga
     9841 tatgccgcac cgaaagccgc agccgtaaaa agcaggcccg ccgtaccggc tgccgcgcaa
     9901 ccgcccgtac agtccgcacc cgtcgacatc aacgcggcga cgcatactat tgtgcgcggc
     9961 gacacggtgt acaacatttc caaacgctac catatctctc aagacgattt ccgtgcgtgg
    10021 aacggcatga ccgacaatat gttgagcatc ggtcagattg ttaaagtcaa accggcagga
    10081 tatgccgcac cgaaaaccgc agccgtagaa agcaggcccg ccgtaccggc tgccgtgcaa
    10141 acccctgtga aacccgccgc gcaaccgcct gtgcagtccg cgccgcaacc tgccgcgccc
    10201 gctgcggaaa ataaagcggt tcccgcgccc gccccgcaat ctcctgccgc ttcgccttcc
    10261 ggcacgcgtt cggtcggcgg cattgtttgg cagcgtccga cgcaaggtaa agtggttgcc
    10321 gatttcggcg gcaacaacaa gggtgtcgat attgcaggaa atgcgggaca acccgttttg
    10381 gcggcggctg acggcaaagt ggtttatgca ggttccggtt tgaggggata cggcaatctg
    10441 gtcatcatcc agcataattc ttccttcctg accgcatacg ggcacaacca aaaattgctg
    10501 gtcggcgaag gccagcaggt caaacgcggg cagcaggtcg ctttgatggg caataccgac
    10561 gcttccagaa cgcagcttca tttcgaggtg cggcaaaacg gcaaaccggt taacccgaac
    10621 agctatatcg cgttctgact ttctgcctga aatcaaattg gaaatgccgt ctgaaaggtt
    10681 ttcagacggc atttttgttt ttggcggctg tgttttcaaa tcattcagaa gggacggggc
    10741 atataaattt gtttgactgt gtttgg
//
LOCUS       DAWFIE010000066        10236 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWFIE010000066 DAWFIE010000000
VERSION     DAWFIE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10236
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.066"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(87..161)
                     /locus_tag="WGQ71_001579"
     tRNA            complement(87..161)
                     /locus_tag="WGQ71_001579"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(126..128),aa:Glu,seq:ttc)
     gene            complement(179..255)
                     /locus_tag="WGQ71_001580"
     tRNA            complement(179..255)
                     /locus_tag="WGQ71_001580"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Arg,seq:acg)
     gene            complement(365..2038)
                     /gene="recN"
                     /locus_tag="WGQ71_001581"
     CDS             complement(365..2038)
                     /gene="recN"
                     /locus_tag="WGQ71_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ0707561.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKSVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSREQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(2222..2824)
                     /locus_tag="WGQ71_001582"
     CDS             complement(2222..2824)
                     /locus_tag="WGQ71_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ0707562.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            complement(2925..3866)
                     /gene="ylqF"
                     /locus_tag="WGQ71_001583"
     CDS             complement(2925..3866)
                     /gene="ylqF"
                     /locus_tag="WGQ71_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ0707563.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKIWLEHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEEGGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3947..4801)
                     /gene="rapZ"
                     /locus_tag="WGQ71_001584"
     CDS             complement(3947..4801)
                     /gene="rapZ"
                     /locus_tag="WGQ71_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ0707564.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIAYLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIEKFLTHWLPRLEDESRSYVTVAIGCTGGQHRSVYVVEKLARRLKGRYELLIRHR
                     QAQNLPSR"
     gene            complement(4782..5744)
                     /locus_tag="WGQ71_001585"
     CDS             complement(4782..5744)
                     /locus_tag="WGQ71_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ0707565.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(5746..6195)
                     /gene="ptsN"
                     /locus_tag="WGQ71_001586"
     CDS             complement(5746..6195)
                     /gene="ptsN"
                     /locus_tag="WGQ71_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ0707566.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6380..7270)
                     /gene="ubiA"
                     /locus_tag="WGQ71_001587"
     CDS             complement(6380..7270)
                     /gene="ubiA"
                     /locus_tag="WGQ71_001587"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ0707567.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFTANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7300..7776)
                     /locus_tag="WGQ71_001588"
     CDS             complement(7300..7776)
                     /locus_tag="WGQ71_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ0707568.1"
                     /translation="MEHLFGKWLPDLPTPISDGISLPMVRLLHTRSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7791..8438)
                     /gene="bioD"
                     /locus_tag="WGQ71_001589"
     CDS             complement(7791..8438)
                     /gene="bioD"
                     /locus_tag="WGQ71_001589"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ0707569.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADEQRLTMPEIFSYPASPHLAARLDGRDLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLYSLVFNHIHDSRDAHIAQDSLNYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8435..9736)
                     /gene="bioA"
                     /locus_tag="WGQ71_001590"
     CDS             complement(8435..9736)
                     /gene="bioA"
                     /locus_tag="WGQ71_001590"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ0707570.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMVVQYQQARGLRAKQNMATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDGADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            complement(9950..10189)
                     /locus_tag="WGQ71_001591"
     CDS             complement(9950..10189)
                     /locus_tag="WGQ71_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707571.1"
                     /translation="MNVWDIWGRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMSESPDFANAVAMAVAMICIVWIAVLSMTD"
BASE COUNT         2376 a         2788 c         2604 g         2468 t
ORIGIN      
        1 gagaaatgag aaatgagaaa tgagaaatga gaaatgagaa atgagaaatg agaaatgcct
       61 ccgatataat cggaggcatt ttgaattggc acgcccacgg ggaatcgaac cccggttacc
      121 gccgtgaaag ggcgatgtcc taaccgctag acgatgggcg catattgtat tttcttactg
      181 gcgcacccgg agcgattcga acgcccgacc ctctggttcg tagccagata ctctatccaa
      241 ctgagctacg ggtgcagcac aatcacttgt tcaagtgatt aagaaagcgt gataatacgg
      301 atttatctga gttctgtcta ttgttttaga cttttatttt tagttatttt ttaactgatt
      361 gattttaaaa taaactattt ttcgacgcaa gttgcagcaa ttctgccgca tgttgccgcg
      421 tcgtatcggt aatgacttct ccgcccaaca tacgggcaac ctcttcgatc cgttggattt
      481 catccaatat actgatttcg ctgacggttt gctctccctc gctgtgcttg cgcacccgcc
      541 agtggttttc tccgcaggat gcgacttggg gaaggtgggt aacggcaagc acctgatgtt
      601 ttctgcccaa cgcacgtaat gccttgccga ccatttcagc cactccccct ccaataccgg
      661 tatcgacctc atcaaaaatc agggtgggaa cttgggtata ttggctggca acaacctgta
      721 aggcaaggct gatacgcgcc aattcgccgc cggaggcaac tttattcagc ggacggggcg
      781 gattgccttt gttggcggca acttgaaatt gaacctgctc caaaccgtgt gccgtcggcg
      841 acgagggcaa caggacgatg tcgaaacgtg cacctttcat ggcaaggtgt tgcatatgct
      901 cggtcgtttc gccgctcaaa cgctctgccg cctgatggcg catggcagaa aggatgtggg
      961 cagcttcctg atattcggca aaattgtggg caacattatg ctcgagcgcg tccaaatcgg
     1021 cggcagcttg caggctttgc aggcgttctt cgatttctgc caacttggca ggcaactctt
     1081 caggctcgat ccggtatttc cgcgccatcc ccatcagctc gcccatgcgc tgttcctgtg
     1141 ccgccaattc attgggattg atgtcgctgc gacctgccac atcgcgcata ttggcactga
     1201 tttcgcccaa ttcggcttcg atgcttgcca acatattcag gctctcggca aagcgcggct
     1261 cgatgttttg cagattggcc aatagttttt gacattgata gatatggcgt tggatgccgt
     1321 tgtcgccgtc aatcttgctt ccgacttctt cggcagcctg caacagctcg gcagaatggg
     1381 caaggctgtc gtggctttgg ctgagggctt cccattcgcc ttgtttaatg tccaactgat
     1441 tcagttcgtt aaactgccat tccagacgct cccgctcgat aatgacggca tcggcgtgtt
     1501 cctgcgcctc ttggagggct tttttcgcat tggcccaatt ttgataaagc tgcctgacgg
     1561 tttccgcctg ctccctgcta cccgcaaatg cgtccaacaa ttcgcgctgg gcggcttcct
     1621 gattaagcga atgatgggcg ttttgcccgt ggatgtcgat aagctggcta ccgacggatt
     1681 tgagttgcgc caaggtagcg gcctgattgt tgataaagct gcggcttttg cctttggcat
     1741 cgataatgcg gcggatactg agttcttctt ctccgtcgtt taaaagcccc tgttcataca
     1801 attctgcttt taaaacaggc aaatgggaaa tatcgaacaa tgccgacaac tgcgcttctt
     1861 ttgcaccgct gcggacttgg ctgtaatcgg ctttgtcgcc caacagcaga ccaatcgcat
     1921 ccaaagtaat ggacttgccc gcgccggttt ctccggtcaa tacggtaaag ccgctttgaa
     1981 aatccagatt cagattttca acaatgacaa aatcacgcaa agaaagtgtt agaagcattg
     2041 tgaggattcc ttagcaattc taccgacaaa agcaatatca actttagata tacgaattat
     2101 aactaagatt tttttaaatt aaaaataagt tttccttaat tttgcttagt acttgttttt
     2161 atctgcttat ttgttgcaat gccgtctgaa gcaatgtgcg tttcagacgg catttggaat
     2221 ttcagttggg cagggtatcg gacggtacgg cttccggctc ttcttcatcc gtaggcggca
     2281 tttccattaa atcgggtaaa accagttcgc ccagttcggt cagcggcgga agttcttcca
     2341 gaccgtccaa gcgcaaatcg ctgagaaaag ttgccgttgt cgcccacaat gcgggttttc
     2401 ccaatgtgtc ccgatgtccg atgacttcaa tccacccccg atcctgcaag gtctgcatca
     2461 cgttctgcga caccgccacg ccgcgtatgc cctcgatgtc gccgcgcgtt acgggctgct
     2521 ggtaggcgat aatcgccagt gtttccatca cggcgcggga gtaacgcggc gcacgctgtt
     2581 cttgcaggct gcccagccgc tcgaatgccg tctgaacaat ctgaaaacgc cagccctctt
     2641 gcgtatgcac cagttgcaac gccctatcct gccaacgcgt tttcaactgc gccaacacat
     2701 caatcagttt gtcctgcgac agtggcggca cacacaattc gcgcatagat ttttcggtca
     2761 gcggttcggt ttgggtcagc agtgcggctt caattagtgc gtcgggagaa attttgtcgg
     2821 tcatacgggt atccgtgctg aaacggcatg ggtttggata tgccgtctga aatcggttgg
     2881 agtagagaga agctgcctga aaaatatttt tcagacggca ttctttatgc ttccgaaggc
     2941 ttctgccctt tacgctctgc ttttctggct tcgcgtatgg ctttgagttc ggcttctttc
     3001 tgacgggctt ttttgagcca agtttcccat tggttcggcg tttcgagggt gattctgccg
     3061 attttgcctt cacggaagtc ggtgaggatg ttttcggcgg ctttttggta gttgatccgt
     3121 ccgccgctga ggactgcgcc gcgttttttg gctatccatt cgagccaaac gttttcgtcc
     3181 cagtggctgc tggggtcttt gtcggcttgg tagcgttctt gcaacatagg gaggtagtgg
     3241 cggcggaggt agtctaaaag ttcgagggcg acttcttctt cgtccaacgc gttgcgtccg
     3301 actgcgccgc cggcggcaag gttgtagccg ccttcttcga cgatgatttt cggccacagc
     3361 attccggggg tgtcgtagag ccagaagtca tcggcgagga agaggcgttg ttcggctttg
     3421 gtgatgccgg gttcgttgcc ggttttggcg gattttttgc ctatcatgcc gttgatgagg
     3481 gtggacttgc caacgttggg gatgccgcag atgaggacgc gcaggggttt atctatgcct
     3541 tggcggtggg gaatcatggc acgacaggct tgggtaattt tgccgtgtgc gcctgtttcg
     3601 gaggaatcga gggcgatggc gcaggtgtcg gggcggctgt tatagtgttc gagccagatt
     3661 ttggtgcgct cggggtcggc aagatcttgt ttgttgagga ttttaagttt aggtttgcct
     3721 ttggaaagtt gggcaagcag ggggttttcg ctggaggcgg gcattcgcgc atccagcatt
     3781 tcaatcacca tgtctacgct tttggcgcgc tcggcgatgg cttttttcgc tttgttcata
     3841 tggccgggaa accattggat tgccatgtct ttcctttctt ctcaatattt gaaatgccgt
     3901 ctgaaacaga aaatcgggtt tcagacggca tcatcgttca acgggattag cggctcggca
     3961 ggttttgcgc ctgtctgtgc cgtatcagca attcataacg ccctttcaac cttcgggcga
     4021 gtttttcgac aacatagacg gaacggtgct gcccgccggt acagccgatg gcgacggtaa
     4081 cgtagctcct gctttcatcc tccaaacgcg gcagccaatg cgtcagaaat ttttcgatgt
     4141 cgtcaaccat ttcctgcaca agcggctgtc cgtccaaata atcccaaacg ggcttgtcca
     4201 taccggtgta aggcctcaac tcgggatcgt aatacgggtt gggcagactg cgcatatcga
     4261 acataaaatc cgcgttgttc ggcacgccgt atttgaaccc gaaggactcc aaaatcacca
     4321 gcagccctgt acgttcgacc ttcagccact gccggactgc atggcggagc tgttgggcat
     4381 tcatcttgga agtgtcgata caataggcga tttctttaag cgggaacagc cattcccgtt
     4441 ctttctttaa gctttccaac agggtcatat cctgattgct cagaggatgt cctcgcctgg
     4501 tttcggaaaa ccggcggacc aacacgcttt cttccgcctc gacaaacaaa acttcaaccc
     4561 tgtgccccag tccgcgcaga taggcaatct gttcccgcgc ctgtccgatg tcgatgccgg
     4621 aacgcacatc gacgctgacc gccaattcag tttcgtccgc acgctcgata tgatacgaca
     4681 ccagcgcggg caacatttcc aaaggcaaat tgtccacgca gaaataaccc gaatcttcca
     4741 tttggcgcag tgcgacggat ttgcccgaac cggacaaccc gctaatcagg acgattttca
     4801 tgattctgtt cgttttcttt aagttgcgtc tgatggcgtt ccaaaaattc gcgcgtactg
     4861 tccttaccgc gcaactgcaa aatgtaattg cgtaccgccg cctcaaccaa aacggcgagg
     4921 ttgcgtccga cggcgacggg cagcgtaacc gaacggacgt tgacgttgag gatggattcg
     4981 gtttcggtgc ggatgctcaa ccggtcaagc tgcttcatat actcgtcgtc cgcctcgact
     5041 aaattgataa tgagttgcag gatttttttg gggcggatgg aagtttcgcc gaaaatatgg
     5101 cggatattga gtatccccaa gccgcgcact tccaaaaaat cgcgcagcat aggcgaacaa
     5161 cgcccttcca atgtttccgg gccgatgcgg aacagctcga ccgcatcgtc ggcaatcagg
     5221 ctgtggccgc gcgaaatcag ttccaatgcc aattcgctct tacccaggcc ggaatgcccg
     5281 gtaatcagca cgccgatttc aaacacatcg agaaatacgc cgtgtttgac ggacgatgcc
     5341 gccaaggtgc gttgcaggta aatccgcaac acgtccatca gataggggct ttcgagtttg
     5401 gaagtcagca gtggaatatc gtttttatga caatagtcgc gcagtcccgg ggaaaccggc
     5461 aagccgtttg ccacaatgac caaagacatg gaaatatcga acaggtctcc gaactgataa
     5521 cccgtttccc ccgattcgag acggttcaga tattccgact ctgccaaacc gaccacttgg
     5581 atttggttgg ggtggataaa attcaggtgt ccgaccaggg cgaggacggg cttgtccgcc
     5641 tctacaccga tacggttgtc cgcacccgaa ttgccggcgg cccaggcaag ttgcagtttg
     5701 tattggttgt cgtcaaacag ccggcggaca gagatactgg gcatattatt cttcagtcag
     5761 gatggcgcgg gcttcttccg cagaagaaac cgtcatcagc gattctctga tgcttttttg
     5821 ggaaaacttg ccggccagtt tggacaatac ttccaaatgc tcgcccgtcg cattttccgg
     5881 aaccagcaag ataaaaacca gggaaaccgg cttgccgtcc ggtgcgtcaa atccgacggg
     5941 ttcgcgcgtg cggatgaacg cgcccgtcgc ctgcttcacg ccggcatgac ggccgtgcgg
     6001 gatggcaacg ccctgcccca aaccggtcga accgagtttt tcacgggcaa aaagacattc
     6061 gaaaacatca gcatgggaca atgaggattc gcgttccaaa agcaggcccg cttcctcaaa
     6121 cagccttttt ttactgccca cctccatatc caaaacaata tgggacaaag gcaaaatttc
     6181 gccgataagg ctcataagct tctcttttca gacatcgcaa aacagaaaga ttgtaccgac
     6241 tgctagggca aatctcaatc ccgcatacgg tacgggctga cataacacag cgttttaaaa
     6301 acatatttta acgcttttcg gcacagatag aaatgccgtc caaagcagtt tacggctctt
     6361 cagacggcat tgccctgcct tatttcgcga aaaacgtatg ggcaaaaatg gcggtaaacc
     6421 acacccaacc aattctgttg tttgccaaaa acgtttcaaa acagatttgc cggacgcggc
     6481 ttttgatggc ggcatattgg cggtattgca gcagcaggac gatggggatt gccgtccaat
     6541 atgcccaagc cgcgccgata accgcaccca atactgccat cagcagggta aagccgccat
     6601 gacacagcat aacagcggcg atgtcgtaac gcccgaatgt gacggcggag gttttgatgc
     6661 cgattttcaa atcgtcttct ttgtccgcca tggcgtaaac agtgtcgtaa gccagtgtcc
     6721 ataaaacatt ggcggtaaaa agtatccatg cttcaacggg gacgctgttg cccactgcgg
     6781 caaacgccat cgggataccg aaggaaaagg caagcccgag atagagttgg ggaatcggaa
     6841 aaaaacgttt ggtaaacggg taagtcagcg caagaaacag cgcgggcagg ctcatcagcc
     6901 aagtcagatg attcagcgga atcaggcaca atgcggcaag caggcacaaa aatgccgtca
     6961 gcagcagcgc ttcttttttc ttgaccctgc cctgtgcgaa cggacggttt ttagtgcgct
     7021 cgacagcccc gtcaaaatcg cggtcggcaa aatcgttgat gacgcagccg gcactgcgca
     7081 ttaaaaacgt accgattgta aacgccgcca ataccgccaa atcgggaatg ccgtctgaag
     7141 ccagccacaa tgcccagtag gtcggccaca gtaaaagcag cgtcccaatg ggcttgtccg
     7201 cccgcatcag gcgcaggtac acatccaaac ggtcggacag tcgtaaaaat aaaggggatt
     7261 taggattcat attgccgcac agcttgaaaa aacggtattt tatccgataa agcgtttcag
     7321 ttcgggcaga aaatactcgg tcagcagcat ttcctcgccg tgacgggaaa accgagaacg
     7381 ccgcgcggca aagtaccgtc cgcatccttc gccggaaacg gcaaactcaa acgccgaacg
     7441 cgccccttcc aaatcggctt gaaacagacg ctcgcccaaa gggcgcgtgc cgcagtccaa
     7501 aatgttttgc caaaacgccg aacctatccg gcactcgctc cttgccgcga caacagggat
     7561 accgtccagc ttcaacaaaa cttcgcgcac cagcctccct ccgcattccg tctccaattc
     7621 gcccagtttc agcagttcca ccgaaaatgt atgcggcaag gcgcgcaatg cggcggtcag
     7681 cgaccgggtg tgcagcagcc gcaccatcgg caggctgatg ccgtctgaaa tgggggtggg
     7741 caagtcgggc agccatttcc caaataggtg ttccatattt ttcccaatct ttataccgcg
     7801 tctgtttttg ccaactccat ccattccgct tcggaaaaat cggctttcag acggcatttc
     7861 aagtagttca ggctgtcttg ggcgatgtgt gcgtcgcggc tgtcgtggat gtggttgaac
     7921 acgaggctgt acaagcgaat gccgtattgt ttgagcgctg cgaaactgag taaagtgtgg
     7981 ttgatactgc caagccgtcc gctggtaacg aggatgacgg gatagccttg ctgacggata
     8041 taatcaatgg ttaacaggtt ttccgtcagc ggaaccatca atccgcccgc gccttcgacc
     8101 aaaacgactt cgtactgcgc cgccaattct tgtgtggcgg tacggatttt gtccaagtcc
     8161 aaatccctgc catccagacg ggcggcaagg tggggcgagg cgggatagct gaagatttcg
     8221 ggcatggtca gacgctgttc atcggcttcc tgcatgggta tgcccatgat tttgcggtga
     8281 acggcgatgt cttcggcgat gtcttggcaa ccggtttgca cgggcttttg cgtgatcacg
     8341 cttttgccct gctgcaacaa ttgttttgcc aacataccgg tggcgacagt tttgccgatg
     8401 tccgtgtcta tgccgctgac gaagtaaacg cctttcattt gctgtgttcc ttcaagattt
     8461 gcacggtttt gtcggcaagt ttggtcaaaa cgccgtctga aataatatac ggcggcatca
     8521 gatacaccag cctgccgaac gggcgcaccc aaatgccctg cgccacgcaa tctgcttgaa
     8581 aacgcgccat atccacgcct ttatccagct cgatcacgcc gatggcaccc aaaacgcgta
     8641 cgtctttcac gccgcgaatg tcccacgcgg ctttcagacg gccttttaag atgctttcaa
     8701 tgcggcggat atttgcctgc cagtcttgag acaaaagcag tttgaccgaa gcgcaggcaa
     8761 cggcacacgc cagcgggttt gccataaacg tcgggccgtg cataaacacg cccgcttcgc
     8821 cgcgcgaaat cgtttcggta actttttgcg aagtgattgc tgccgccagc gtcatatagc
     8881 cgccgctcaa acccttgccg atgcacataa tatccggcac gacctccgcg tgttcgcagg
     8941 caaacatctt gcccgtgcgc ccgaatccgg tggcgatttc atcaaaaatc aaaacgatat
     9001 caaactcgtc gcacaaatcg cgcaatccgc gaagatactg cggatgataa aaatacatgc
     9061 cgcccgcgcc ctgcacgacc ggctctaaaa taaaggcggc aatatccgca tgatgcactt
     9121 caaacaaggc gcggacaggc tgcaaatccg ccccgtccca ttcatcgtcg aaacggcttt
     9181 tcggattatc gacaaaataa cgctgcggca acgcgctgcc gaaaatatgg tgcatccccg
     9241 tttccggatc gcagacggac atcgcgttcc aagtatcgcc gtgatacccg cgccgtaccg
     9301 tcgccatatt ttgttttgca cgcaaacccc gcgcctgctg atattgcacc accatcttca
     9361 acgcgacttc caccgaaacc gaacccgaat ccgcataaaa aatacggtcc agcccctgcg
     9421 gcaaaatccc gaccaataat ttgcctagct ccaccgccgg ctcatgcgtc aaaccgccga
     9481 acatcacatg cgacatctgt ttcatctgcg tctcaaccgc ctgattcaaa acaggatgat
     9541 tgtagccgtg tatcgcacac caccaggagg acatcccgtc aatcaaccgc gtgccgtccg
     9601 ccaattcgat aaacacccct tctgcacgtt tgacaggata aacgggcagc ggatcggtca
     9661 tggaagtata gggatggagc agatgggtgc ggtcgaaatc aagcaatgat gatgtgtgtt
     9721 gatgttcaga cggcataagt ttctctcttt tcttattgct gcattcaaac gcaaaacgcg
     9781 tattctactc cgacagaccg cttcccacac ctttccatcc gtttcgggcg taaaaccacg
     9841 caacaaatcg tccgcagtat aagcgcacac cgtttcgcat tccccaagcc cgattggaat
     9901 cagacggccc aacgcccaat accgtttcac gcagcctccg cccaacgctt caatcagtca
     9961 tagacaaaac ggcaatccat acgatacaga tcatagcaac agccatcgca acagcgttgg
    10021 caaaatcagg ggactccgac ataggcgcat agcatctgcc gatgcacggc tcctcattcg
    10081 gctctatgaa taccataccc atcacaaaat ccaccgccaa aaccaggcac ggcttcttat
    10141 acttatgata gatttccacc atcctgcccc atatatccca aacattcata ccgcatatcc
    10201 cgcaggcaac aaattccgat tgaaggttac agccct
//
LOCUS       DAWFIE010000067        10116 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWFIE010000067 DAWFIE010000000
VERSION     DAWFIE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10116
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.067"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(216..1433)
                     /locus_tag="WGQ71_001592"
     CDS             complement(216..1433)
                     /locus_tag="WGQ71_001592"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ0707572.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDQKMRADLADGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1635..2363)
                     /gene="rph"
                     /locus_tag="WGQ71_001593"
     CDS             complement(1635..2363)
                     /gene="rph"
                     /locus_tag="WGQ71_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ0707573.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2445..2909)
                     /locus_tag="WGQ71_001594"
     CDS             complement(2445..2909)
                     /locus_tag="WGQ71_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ0707574.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3126..3902
                     /locus_tag="WGQ71_001595"
     CDS             3126..3902
                     /locus_tag="WGQ71_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ0707575.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPECQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4147..5001
                     /locus_tag="WGQ71_001596"
     CDS             4147..5001
                     /locus_tag="WGQ71_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ0707576.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFISLLELAKEGLVRIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5042..6250
                     /gene="pncB"
                     /locus_tag="WGQ71_001597"
     CDS             5042..6250
                     /gene="pncB"
                     /locus_tag="WGQ71_001597"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ0707577.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDDPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6342..8060
                     /gene="argS"
                     /locus_tag="WGQ71_001598"
     CDS             6342..8060
                     /gene="argS"
                     /locus_tag="WGQ71_001598"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ0707578.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIADSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQAVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDANAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8598..10016
                     /locus_tag="WGQ71_001599"
     CDS             8598..10016
                     /locus_tag="WGQ71_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0707579.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKITGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPALYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAACAAVVAAAGYFTGHL
                     SYYDRGPMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGVEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLNFKIK"
BASE COUNT         2325 a         2960 c         2543 g         2288 t
ORIGIN      
        1 tctaaggtgc tgaagcacca agtgaatcgg ttccgtacta tttgtactgt ctgcggcttc
       61 gtcgccttgt cctgattttt gttaatccac tataaattta atccactata ttttttgttc
      121 caaagtcaaa atatgccgtc cgaacattcg ggcggcagac aaaacggcac tgcccgataa
      181 aggcagtgcc gttgtccgtt tcaaaccgtg aaacatcagc ccaaattaaa ggctttatgc
      241 aataccctgg ttgccagttc catgtatttt tcatcaatca atacggaaac tttgatttcg
      301 gaggtggaaa tcatttggat gttgatgcct tcttctgcga gggtacggaa gattttggcg
      361 gctacgccga cgtgcgaacg catacccaaa ccgactgcgg agactttgca cacggtgtcg
      421 tcgccgtcga tagaagccgc gccgatactg tcttgacgtt ccgacaggat ttccaaagtc
      481 tgtttgtaat cgccgcgcgg tacggtaaag gaaaaatcgg ttgtgccttc gctaccgaca
      541 ttttggataa tcatatcgac ttcgatgttg gcatcggcaa ccgcgcctaa aatctgatag
      601 gcaacgccgg gtttgtcggg tacgccgcgc acgttgatgc gggcttggtt tttatcgaat
      661 gcgataccgg ttacggcagc tctttccatg ttgtcgtcct cttcaaaggt aattaaggtg
      721 ccattgccgc cgtcttgcag gctgctcagt acgcgcaggc gcactttgta ttttccggca
      781 aattctactg aacggatttg caaaactttc gaaccgaggc ttgccagctc aatcatttct
      841 tcaaatgtaa ccgtatccat gcggcgcgct tcgggtacga cgcgggggtc ggtggtataa
      901 acgccgtcta catcggtata gatttggcat tcgtccgctt taagggctgc ggcgagcgca
      961 acggcggaag tgtcggaacc gccgcgtccg agcgtggaaa tatcgccttc gctgctgatg
     1021 ccttggaagc cggcaacgat gacgactttg ccatcggcga ggtcggcgcg cattttttgg
     1081 tcatcaatgc tttcgatgcg ggctttggtg tgggcggtat cggttttgag ggcgacctgc
     1141 cagcctgtgt agcttttggc atccacgcca atgtctttca acgccattgc caaaaggccg
     1201 atggttactt gttcgccggt ggacaaaacc acgtccaatt cgcgcggatc ggggtgttcc
     1261 tgcatttcgt gcgccaacgc gaccaggcgg ttggtttcgc cgctcatagc ggatacgacg
     1321 acgacgatat cgtgtccttc ggcgcgggct ttagcgacac gtttggctac gtttttaatg
     1381 cgttcgggcg agcctacgga tgtgccgccg tatttgtgta cgattaacgc catatttcgt
     1441 gctttcttgt gtgggattgg tcgggcagca tagtttgctg gaaaagcggc tattattact
     1501 attttttaca tgaagttcaa gaacggactg cgctttcccg cctgccgttt gacagcggtc
     1561 agcgaaaaac ctgttctttc agattgttga caaaatgccg tctgaacggt tttcagacgg
     1621 catccggacg acaatcaggc ggcggacaac gcattttgct ggtgttgcag cagttcgcct
     1681 atgccttttt gcgccagtgc aaccagtttg cccaattcgt ccaaactgaa cggcgcgtct
     1741 tccgccgtac cttggatttc gatgattttt cccgatgcgg tcatgacgat attcacatca
     1801 ctgtcgcaac cggagtcttc gggataatcc aaatccaaaa gcggcacgcc gttcactacg
     1861 cctactgaca cagcggcaac ggcttcgcgg atggggtttt cactcaaaat gccgtctgaa
     1921 accagtttgc cgacggcgat ttgcagcgcg acaaacgcac cggtaatcga agccgtacgc
     1981 gtaccgccgt ctgcctgaat cacgtcgcag tcaatcaaga tttgtcgttc gccgagtttt
     2041 tccatatcca cgaccgcgcg cagcgaacgc ccgataaggc gttggatttc ttgtgtgcgc
     2101 ccggactgtt tgcccgccga agcttcgcgg cgcatccggg aagcagttga ggcaggcagc
     2161 atcccgtatt ccgccgttac ccagccttgg tttttaccgc gcagaaacgg cgggacgttt
     2221 tcatctatgg aagcggtaca aatcactttg gtattgccgc attcaataag gcacgaaccg
     2281 tccgtatgcg gcaggaaatg aggggtgatt ttgatatcgc gcaggctgtc cgcggcgcgc
     2341 gagatgcgga tgtaatcagg catactgccc tcccgttaaa aacagataaa ttaaaaagcc
     2401 ttaaatatga aaaatcacat ttaaggcctt caaactgaaa atttctacgc ctcttcggct
     2461 ttgctgcgga taatcaaaag cggcaggtgg ctttggcgca ttaccgtttc ggcaaaactg
     2521 cccattaaaa ggtgcatcag tccggtacgt ccgtgcgtgc ccaacaccag caggtcggca
     2581 ccgttttcat cggcataatc aaccaaatcc tgcgccattt cacgcgcacc cttattggca
     2641 accagcaggt gtttgacggt attttccaca cccagttccc gggcggtgcg ctcggcggca
     2701 tccaaaactt cgttgccttg cgcgacggcg gcggcttcgt agctttcgtg ttgcaaaaat
     2761 tcgggggcga gcgccatata ttcggcagga ttggcaacgt gtaccaaagt cagacgcgca
     2821 ccgttgaccc cggcaagctc ggcggcatgt ttcagggcat tgatggacgt ttcactgccg
     2881 tcaacggcaa caaccaaatg tttgtacata tcgtattctc cttttgcacc gcctcgcggt
     2941 gccctcttgt cggatgggcg cagggacagt ttgcgctgtt tcattataga cccgccgtcg
     3001 ggctttatac aacagccgaa cagcccgacc gctttccagt ataatatgcc gcttccgtgc
     3061 agtcaggcat tttttgccgg ctttcgttca ctttttgatt tgacgcaatc ttgcaggatt
     3121 cgaccatgtc cgacaacgct ttgacctctt cgcgacgctt cggcggcatc gccagactct
     3181 acggagactc tgccttggcg cacttttcac aggcacacgt ctgcgtagtc ggcgtgggcg
     3241 gtgtcggctc gtgggcggtc gaggctttgg cgcggacggg catcggacgt ttgactttga
     3301 ttgatttgga caacgttgcc gaatcgaatg tcaaccgcca gctgcacgcc ctgaccggag
     3361 acttcggcaa agcaaaagtt accgccttgc gcgaacgcgt cgcccaaatc aacccagaat
     3421 gccaagtgtt tgaaattgaa gatttcgtta ccgaagacaa tttgccggaa tacttcggaa
     3481 aaggttttga tttcgtcatc gacgcgatcg accaagtgcg cgtcaaagca gcaatggcgg
     3541 cttattttgt ggaacgcaaa caaccgtttg tcctcagcgg cggcgcgggc ggacaaaaaa
     3601 atccggcgtt aatccaaacc gccgatttga gccgcgtaac ccacgacccg ctgcttgcca
     3661 acctgcgcta caccttgcgg aaacgctacg gattcagccg cgatacgaaa gcaaatatgc
     3721 gcgtgccttg cgtgtattcg accgaaaata tcgtgccgcc gcagtctagg gaggcttgtt
     3781 cggcagatgc cgctccgcaa ggcttgtcgt gcgccggcta cggtgcaagc atgctcgtta
     3841 ccgcttcgtt cgggctatat tgcgcacagg cggcggtgga acacatcgca gacaaaaaat
     3901 aagcaatgcc gtctgaaaca ggattcagac ggcatttgaa caaacgcggc aatcaagctg
     3961 tcggttcaga aaaaccaaca tcggcacgcc taaaaccgta ttcacaacct gctccttttc
     4021 aaaacatttg catttaaaag ccgttataat gccgtctgaa catctgcccg accacattat
     4081 acgtgaatgt cggcagattg ttttcttttg taaacttata ttaaaatcca cttaccgatt
     4141 cacgccatgc cgcccatccc tgccccatct gcaccatccg agcacaccgt cgcatgggta
     4201 ttcggtcaac ccgttaccga tttgccgcag gatttgttta tcccgcccga cgcgctgaaa
     4261 gtcgtattgg gcagttttca aggccctttg gatctactgc tgtatctgat ccgcaagcag
     4321 aacatcgatg ttctcgatat tccgatggtg aagattaccg agcagtatct gcactacatc
     4381 gcccaaatag aagcctatca gtttgatttg gcggcggaat atcttttgat ggcagcaatg
     4441 ctgattgaaa tcaaatcgcg cctgctgctg ccgcgtaccg aagccgtcga agacgaagaa
     4501 gccgacccgc gtgccgagtt ggtgcgccgc ctgttggctt atgaacagat gaagctggcg
     4561 gcacaagggt tggatgcgct tcctcgtgcg ggccgggatt tcgcatgggc gtacctacct
     4621 ctggaaattg ccgtcgaagc caagctgccc gaagtctata ttaccgactt gacgcaagcg
     4681 tggctgggta ttttgtctcg ggcaaaacat acgcgcagcc acgaagttat caaagaaacc
     4741 atctccgtgc gcgcgcaaat gacggcaatc ctgcgccgtt tgaacggaca cgggatatgc
     4801 aggtttcacg acctgttcaa tcccgaacag ggcgcggctt acgtggtcgt caacttcatc
     4861 tcactgttgg agcttgccaa agaaggattg gtcagaatcg tgcaggaagt cggtttcgga
     4921 gaaatccgaa tcagcctcaa tcatgagggg gcgcattcag acggcatttc cggcacacgg
     4981 ggcgggcgcg atgtgttcta atacgcccca agccgccacc aaaaatcggg agacacgcca
     5041 tatgaccggc atcatacatt cgctgcttga caccgacctc tacaaattca ctatgctgca
     5101 agtggttctg caccagtttc cgcagacgca cagcctttac gaattccgct gccgcaacgc
     5161 ctcgaccgtc tatccgcttg ccgacatcaa ggaagacttg gaagccgaac tcgacgcgct
     5221 ctgccggctg cgcttcaccc acgacgaact cggctatctg cgctccctgc gtttcatcaa
     5281 aagcgacttt gtcgattatc tcgaactctt ccagctccaa cgccgctttg tcgaaatcgg
     5341 cacagacgat aaagaccgtc tgaacatccg tatcgaaggt ccgatgatac aggcgatgtt
     5401 ttttgaaatc ttcatcctcg ccattgtcaa cgaactttac ttccgccggc tggaaacccc
     5461 tgccgttatc gaagaaggtg aacgccggct tcaagccaaa gccgcgcgcc tcaaagaaat
     5521 cgccgccgca caaaaccccg acgatccgcc cttcctgatt tccgacttcg gcacgcgccg
     5581 ccgctacaag ctcgcgtggc aggaacacgt catccgcacc ctgctcgaag ccgccccaag
     5641 catcgtgcgc ggcaccagca atgtctttct cgccaaaaaa ctcggcatta cccccatcgg
     5701 caccatggcg cacgaattcc tccaagcctt ccaggccctc gacgtacgcc tgcggaattt
     5761 ccaaaaggcc gcgctcgaaa gctgggtgca cgaataccgg ggcgatttgg gcgttgccct
     5821 gaccgacgtt gtcggtatgg atgccttcct gcgcgatttc gacctctatt tcgccaaact
     5881 tttcgacggg ctgcgccacg acagcggcga cccttacgtt tggggcgaca aagcctacgc
     5941 ccactatcaa aagctcaaaa tcgacagccg caccaaaatg ctgaccttct ccgacgggct
     6001 ggacatcgaa cgctcttggg cattgcacca atatttcaaa gaccgcttca aaaccggctt
     6061 cggcatcggc accaacctca ccaacgatat ggggcatacg cccttgaata tcgtcttgaa
     6121 actggtcgaa tgcaacgggc agtccgtcgc caagctgtcc gactctccgg gcaaaaccat
     6181 gaccaacaac agcaccttcc tcgcctacct gcgccaagtg ttcgacgtac ccgaacccga
     6241 aacgccgtaa accggcagaa aaagcgcaca attcctgttt ctgccgcata aaatctttta
     6301 aaataccgcc tgatttgaat ttaaccgaaa gaccgaactt catgaaccta catcaaaccg
     6361 tcgaacacga agccgccgcc gcctttgccg ccgcaggcat cgccgacagt cccgttgttt
     6421 tgcagccgac caaaaatgcc gaacacggcg atttccaaat caacggcgtg atgggtgcgg
     6481 cgaaaaaagc caaacaaaac ccgcgcgaac tggcgcaaaa ggtcgccgac gcattggcag
     6541 gcaacgccgt catcgaaagc gcggaagtcg ccggtcccgg ttttatcaac ctgcgcctgc
     6601 gccccgaatt tctcgcgcaa aacattcaga cggccttgaa cgacgcacgt ttcggcgtgg
     6661 caaaaaccga caaaccgcaa accgtcgtta tcgactattc ttcgcccaat ctggcgaagg
     6721 aaatgcacgt cggccacctg cgttccagca tcatcggcga cagcatttcg cgcgtgttag
     6781 aatttatggg caataccgtt atccgtcaaa accacgtcgg cgactggggt acacagtttg
     6841 gcatgttggt cgcttatttg gtcgagcagc aaaaagacaa tgccgcgttc gagttggcgg
     6901 atttggagca gttttaccgc gccgccaaag tgcgctttga cgaagaccct gcctttgccg
     6961 acaccgcacg cgaatacgtt gtgaagctgc aaggcggcga tgaaaccgtt ttggcattgt
     7021 ggaaacagtt tgtcgatatt tcgctctcgc acgcccaagc cgtttacgac acgctgggct
     7081 tgaagctgcg ccctgaagac gtggcaggcg aatcgaaata caacgacgat ttgcaagccg
     7141 tggtcgatga tttggttcaa aaaggcttgg cggtcgaaga cgacggcgcg aaagtcgtgt
     7201 tcttggacga gtttaaaaac aaagaaggcg aacccgccgc atttatcgtg caaaaacaag
     7261 gcggcggctt cctttatgct tccaccgatt tggcgtgtct gcgctaccgt gtcggcacgc
     7321 ttcatgccga ccgcctgctg tacgtcgtcg accaccgcca agccctgcac tttgagcaac
     7381 tctttaccac ttcacgcaaa gccggctatc tgccggaaaa cgtcggcgcg gcatttgtcg
     7441 gcttcggcac gatgatgggc aaagacggca aaccgttcaa aacacgcagc ggcgacaccg
     7501 tgaaactggt cgatctgctg accgaagccg tcgaacgcgc caccgctttg gtgaaagaaa
     7561 aaaatcccga attgggtgca gacgaagcgg caaaaatagg caaaaccgtc ggcatcggcg
     7621 cggtcaaata cgccgacttg agcaaaaacc gcaccagcga ttatgtgttc gactgggacg
     7681 ccatgctctc gtttgaaggc aacaccgccc cctatctgca atacgcctac acccgcgtgc
     7741 aaagcgtgtt ccgcaaagca ggcgaatggg atgcaaatgc gccaaccgtt ttgaccgaac
     7801 cgctggaaaa acagcttgcc gccgagctgc tgaaatttga agacgtactg caaagcgtgg
     7861 cggacacggc gtatccgcac tacctcgccg cctacctcta tcaaattgcg accctgttca
     7921 gccgcttcta cgaagcctgt ccgatactca aagccgaagg cgcaagccgc aacagccgcc
     7981 tgcaactggc aaaactcacc ggcgacacgc tgaaacaagg cttggatttg ctgggcatcg
     8041 atgtgttgga cgtaatgtaa aaccgcaccg cccgattgcg gacaacagcc tcgccatcct
     8101 tatccgaatc tgaaaaaagc ggcgcgatac accgtatccg ccgcccctcc caaaatgcga
     8161 aacaaacaaa cgccaagcaa gcaagcaagc aagcaagcaa gcaagcaaaa aattataacc
     8221 cccttcctgc cgacgcacgc actttccgcg cggcgcattc ccctttttcc gcccctcaaa
     8281 cccgcctttt cttcaggcaa ggtttcagcc cgcctctttt ccctgttttc ctttccccga
     8341 cacgcgtgcg cgccccctgc cgcactgtgc tgcactttcg cgcccggacg gcatcgttcc
     8401 gccatccggt tctctgtttt acatacccct gtttcagaaa gaaatgcaga tgtttcaaca
     8461 cacaggacga catataaagc accgccctat gtgttgcccc gatttggaag gggttacgcc
     8521 tcccaaataa agtctgatcc tgccgccccg aaggacagat gtccgagtgg cgaagtttca
     8581 atcgaaaagg aaatacgatg aatattcacg ccctgctctc caaacaatgg acgctgccgc
     8641 cattcctgcc gaaacggctg ctgctgtccc tgctgatact gctggccccc aatgcggtgt
     8701 tttgggtttt ggcactgctg accgccaccg cccgcccgat tgtcaatttg gactaccttc
     8761 ccgccgcact gctgatcgcc ctgccttggc gtttcgtcaa aattaccggc gtattggcgt
     8821 tttggctggc ggttttgttt gacgggctga tgatggtgat ccagcttttc ccttttatgg
     8881 acctcatcgg cgccatcaac ctcgtcccct tcatcctgac cgcccccgcc ctttatcaga
     8941 taatgaccgg gctgttgctg ctgtatatgc tggcgatgcc gtttgtgttg cagaaagccg
     9001 ccgccaaaac cgacttccga cacattgccg cctgtgccgc cgttgtggcg gcagccggct
     9061 atttcaccgg ccatttgagt tactacgacc gggggccgat ggccaatatc ttcggcgcaa
     9121 acaacttcta ttacgccaaa agtcaggcga tgctctacac cgtcagccaa aatgccgact
     9181 ttattaccgc cggcctggtc gatcccgtct tcctcccctt gggcaatcaa cagcgtgccg
     9241 ccacgcatct gaacgagccg aaatctcaaa aaatcctctt tatcgtcgcc gaatcttggg
     9301 ggctgccggc caatcccgaa cttcaaaacg ccacttttgc caaactgctg gcgcaaaaag
     9361 accgtttttc ggtttgggaa agcggcagtt ttcccttcat cggcgcgacg gtcgaaggcg
     9421 aaatgcgcga actgtgtgcc tacggcggtt tgcgcgggtt tgcactgcgc cgcgcgcccg
     9481 acgaaaaatt tgcccgctgc ctccccaacc gtttgaaaca agaaggttac gccacctttg
     9541 cgatgcacgg cgcgggcagt tcgctttacg accgcttcag ctggtatccg agggcgggct
     9601 ttcaagaaat caaaaccgcc gaaaacctga tcggtaaaaa aacctgtgcc attttcggcg
     9661 gcgtgtgcga cagcgagctg ttcggcgaag tgtcggcatt tttcaaaaaa cacgacaagg
     9721 gactgtttta ctggatgacg ctgaccagcc acgccgacta tcccgaatcc gacattttca
     9781 accacaggct caaatgcacc gaatacggcc tacccgccga aaccgacctc tgccgcaact
     9841 tcagcctgca cacccaattc ttcgaccaac tggcggattt aatccaacgc cccgaaatga
     9901 aaggcgtgga agtcatcatc gtcggcgacc atccgccgcc cgtcggcaac ctcaatgaaa
     9961 ccttccgcta cctcaaacag gggcacgtcg cctggctgaa cttcaaaatc aaataacaac
    10021 aatgccgtct gaacgcacca acagccttca gacggcattt tgcagacaga ccgacccttc
    10081 aagcccactt ttttcatcat ctccgataaa ttgctt
//
LOCUS       DAWFIE010000068         9990 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWFIE010000068 DAWFIE010000000
VERSION     DAWFIE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.068"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            296..1753
                     /locus_tag="WGQ71_001600"
     CDS             296..1753
                     /locus_tag="WGQ71_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0707580.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIVYFTRLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFIPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKCYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
     gene            complement(2225..2584)
                     /gene="rnpB"
                     /locus_tag="WGQ71_001601"
     ncRNA           complement(2225..2584)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WGQ71_001601"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2663..3766)
                     /gene="prfB"
                     /locus_tag="WGQ71_001602"
     CDS             complement(2663..3766)
                     /gene="prfB"
                     /locus_tag="WGQ71_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ0707581.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(3869..4762)
                     /locus_tag="WGQ71_001603"
     CDS             complement(3869..4762)
                     /locus_tag="WGQ71_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ0707582.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIQL
                     GEEAARAALPEIKRKLAAYRY"
     gene            5104..5553
                     /locus_tag="WGQ71_001604"
     CDS             5104..5553
                     /locus_tag="WGQ71_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0707583.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            5627..5935
                     /locus_tag="WGQ71_001605"
     CDS             5627..5935
                     /locus_tag="WGQ71_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707584.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            6051..6893
                     /locus_tag="WGQ71_001606"
     CDS             6051..6893
                     /locus_tag="WGQ71_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ0707585.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            6883..7629
                     /locus_tag="WGQ71_001607"
     CDS             6883..7629
                     /locus_tag="WGQ71_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ0707586.1"
                     /translation="MQPEISAQYQHRFAQAIREGEAADGLPQERLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            7651..8238
                     /locus_tag="WGQ71_001608"
     CDS             7651..8238
                     /locus_tag="WGQ71_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ0707587.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            8244..8423
                     /locus_tag="WGQ71_001609"
     CDS             8244..8423
                     /locus_tag="WGQ71_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ0707588.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            8655..9095
                     /locus_tag="WGQ71_001610"
     CDS             8655..9095
                     /locus_tag="WGQ71_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707589.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQSYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(9624..9980)
                     /locus_tag="WGQ71_001611"
                     /pseudo
     CDS             complement(9624..9980)
                     /locus_tag="WGQ71_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2571 a         2676 c         2238 g         2505 t
ORIGIN      
        1 gcccttcggc gtggcggata tataaggaag tgattttcca tctaagtaaa aaccgcccta
       61 tcggataagc ccttaacaga aaaggcttta cccgcgccgt atcggaacac atcctctaaa
      121 atacaatccg ttgaattgaa aaaaatataa aaacatccgc ccgcgaaaaa cggcagcgcg
      181 tcgtttgaca aagaatgaaa atatcggtta aaaaccgatt ttcatacaaa aaacaccgct
      241 gccgtccgca tccgtttcag acggtattga gagaaaatct tttaggagaa cctttatgtc
      301 ccggcatccc gcccccaccg gagaaaaaac attcttcggc caccccttcc agctttccac
      361 cctcttccat atcgaattgt gggaacgttt ttcattttac ggaatgcagg gcatcctgct
      421 gatttacctc tactacaccg ccgacaaagg cggcttgggc atagacaaaa ccctcgccgg
      481 cggcattgtc ggcgcataca gcggcagcgt gtacctgtcc accattttgg gggcgtggtt
      541 tgccgaccga gtatggggtg cggaaaaaac cctcttcctc tcgggcatcg tcgtgatgct
      601 cggacacatc gtccttgccg ccgccccggg cctgtacggc cttttaatcg ggctgatatt
      661 catcgcattg ggcagcggcg gcgtgaaatc tacggccagt tctatggtgg gcgcattata
      721 cgaacaggac gaaatgcgcc cgctgcgcga tgcgggattt tccattttct acatcgccat
      781 caacatcggc ggcttcctgg gcccgctgct gaccggcctg ctgcaggaaa acatcggttt
      841 ccattatggt ttcggcgcgg cggcggtcgg tatggcattc ggcttgtggc gttattccct
      901 gggacgtaaa aacctgcccc accccaccgt cccccatccg ctttcaaaag gacagggcaa
      961 aactgcggcc gccgtcggca tcgccctcat cgccgcactt gcaaccgcca tcaaaacagg
     1021 gcttgtcaac ctcgacaatt tctccggcat cctcctatct accgtcatcc ttgccgtcat
     1081 cgtctatttt acccggctgc tgaccaaccc ccgcgtcagt tccgacaaca agcggcatat
     1141 catcgcctac atcccgcttt tcctgaccat ctgtatgttt tgggcggtct ggtttcagat
     1201 ttacaccgtg gcaaccgtct atttcgacga aaccgtcaac cgcaccatcg gttcgtttac
     1261 cgtacccgtc gcttggaaag attctatgca aagcctgtgg gtcatcctgt tttccggact
     1321 tatggcggca atgtggacaa aaatggggcg caaacagccc aaaaccccgc tgaaattcgc
     1381 tatggcggta tttgttaccg gcgcgtcgtt tttgggattc atccccttta tttccgccgg
     1441 cacgccgatg cctattgcgg ttttcgcact gattgtcctt gccatcacga taggcgaact
     1501 gatgatttcc ccgattgcgc tgtccatctc caccaaaatc gcaccgcctt tattcaaaac
     1561 ccaaatggtc gcccttaatt tccttgcctt ttcattaggc ttcactttgg gcggcgtatt
     1621 gtttgaaaaa tgctatcagg cgggcgacga aatcggcttc tatcggctgc tgttctacat
     1681 cggcgcagcc acaggcttcc tgctgctcct gctcgtcccc aaattgaaca aaatgctcga
     1741 aggcacagac taagtcccgc cccgatgccg tctgaaccct tcagacggca tttttccgca
     1801 taatgaaacc aaaccgtttc cacccgacag gacaggctcc ccccaaccgg aaggcagcct
     1861 gccgattgtc atttaaatat ctgactgatt ccgtctgttt gcaagggaaa tagccgttta
     1921 tttccgcttg ggcggaaacc ggttctattg agtaaaaggc attttatccg actaaattag
     1981 tgctgcatca acggaatata tagtggatta acaaaaatca ggacaaggca acgaagccgc
     2041 agacagtaca aacagtacgg aaccgattca cttggtgctt cagcacctta gagaatcgtt
     2101 ctctttgagc taaggcgagg caacgccgta ctggtttttg ttaatccact ataaaaacac
     2161 aacctaaata aaaatgccgt ctgaaccata tttcaggttt cagacggcat ttgcgtgtcg
     2221 gatgcacacc ggacaggcgg taagccgggt tctgtctcgg acagtcattc ctctaggcat
     2281 accgttacca gtatgctcaa gcaacctacc cgaacgctcg gcgggcagcg tcattgcgtt
     2341 ctgtttggtc ttgctccgaa tggggtttgg cctgccgcat attgttacca aatgcgcggt
     2401 gcgcccttac cgcacctttt cacccttgcc tgtgctgcca aagcagccat cggcggtttt
     2461 gctttctgtt ccactttccg tcgcgttacc gcgcccggcc gttaaccggc attctaccct
     2521 gcggagcccg gactttcctc cccgtatgcc ttacgcgata cgcggcgact gtctgctcgt
     2581 cccgtgtgcg gcgcggatta taacacgaaa cgcaaaaatg ccgtctgaaa cggtacaggt
     2641 ttcagacggc atacagccta aactacacgc cctgtttcag gctggcttcg ataaagccgt
     2701 ccaaatcgcc atccaatacg gctttggtgt tgccgacttc gtagcctgta cgcaagtctt
     2761 tgatacgtga ggaatccaaa acatacgaac ggatttggct accccaacct acatcggatt
     2821 taccttcttc caacgcctgt ttctcttcat tgcgtttgcg catttccaat tcatacagtt
     2881 tggacttcaa catttccatc gcggcggctt tgttggcgtg ttgcgaacgg tcgttttgac
     2941 attgcaccac aatccccgtc ggctcgtggg taatacgcac ggcggagtcg gttttgttga
     3001 tgtgctgacc gcccgcgccc gatgcgcgat aggtgtcgat acgcaaatcg gcggggttga
     3061 tttcgatttc gatggaatca tcgatttcag ggtaaacgaa cacggaggca aacgaagtat
     3121 ggcgtttgtt gttcgagtca aacggtgaat agcgcaccaa gcggtgaacg ccagtttcgg
     3181 tacgcagcaa accataggcg tattcgcctt ccactcggat agtcgcgcgg ttgatgcccg
     3241 cgatttcgcc gtcgtcttct tcgaggattt cgattttgaa gcctttgcgc tcggcgtagc
     3301 ggctgtacat gcggaacagc atacccgccc agtcttccgc ttccgtaccg cccgcgcctg
     3361 cggtgatgtc gataaagcag ttgttcgggt cggcgggctg gttgaacatc cgtttgaact
     3421 ccaaatccgc catctgtttt tccagccccg ccacatcttc ctgcacggcg gcaaaacctt
     3481 cttcgtcgtt ttcttcgacg gtcatttcaa tcagcatgcg gttgtcttcg atgcccgaag
     3541 cgatgttgtc gagcgtcaac acgatgcctt cgaggatttt gcgctctttg ccgatttctt
     3601 gggcgcgttt cgggtcgttc caaagttcgg ggtcttcgga aagaccgata acttcttcca
     3661 atcggtcttt cttaccctga taatccatat aaactcggat gtcttcgctg cgcttttcca
     3721 aatcattcag ggtattgttg agctggttga ttacttcggc ttccatgatt cttttgttct
     3781 ttcaaaattt taggggcgta ttgtacggga ttcgggtatt tttttctatg gataaagcct
     3841 tctagaaaca cgttcggacg gcatagcgtc aataacggta tgccgccagt ttgcgtttga
     3901 tttcaggcaa tgcggcacgt gctgcctcct cacccaactg gatggcacgt tttttctgat
     3961 cgaatccgcc gactgcaccc aaatccaaaa cctgcggttt gataaccaca tccgcctgcc
     4021 ccaactcatt ttgcaacgcg gaaacgctca ttacgttcag cgtctgatcg agataagaga
     4081 agaagccttg gctgatgttt ttgcccggac gggcggaaat atcgacggca atcacgaaat
     4141 tcgccccctg ccgccgggcg gcactgacgg gcacgggctg cgacagaccg ccgtcaacat
     4201 atgtatgcct gccgatgata acgggttgga acacattggg aatggcggcg gaagcgcgca
     4261 cagcctgccc ggcattccct tgattgaaag cgacggcctt gccggtttca aaatcagtag
     4321 caacagcggc aaatttgatg ggaaactgct gaatctgcct gccgccgact tttcggttga
     4381 tgtaattttg cagcttttcg cctttgataa aaccactggt ggacaaggtt aaatcgacta
     4441 aatcggtttt gcctaaaatt tcggcttcca attcgaggcg gtcgggcgac atacccgatg
     4501 caaaaaggct gccgacaatc gaacctgccg atgtgccggt aaccaccttc acaggaatac
     4561 cgttttcttt caaaacctta ataataccta catgggcaaa tcctttagat gcgccgccac
     4621 cgagtgccaa accgaccact gcggcgggtt tggcggtttg caccggcttg cgggcggcat
     4681 tatttcccgc cgtgccgcag gcggcaagca acgcggcggc ggcgattgcc aaaagcggtc
     4741 tgatttttga aaacgttacc atattttcca ttcctttata tatcgcaccc cgtcaaaaag
     4801 agggattgct tttcttaaca accccctttg acagccaagc aaatgggggc tttgttaagt
     4861 catcatcaaa attaatattt ctttttttcc tttacggaaa ttatatttga aggcatacta
     4921 tccaaggtgg gaattatctc acaacaccgc cgttatccaa atatcccgcc tttttccctt
     4981 cctttccatc aaaatacttt ctttttatat tcattaactt gttaaatcat tggctgccgg
     5041 gtgtcagttt ttccgacaaa atccgtctaa tggggtatca acagaaccaa aacaggaaca
     5101 cttatgaaaa tcggaacaac ttggcagacg gcatccgcta tgctggtttt gcgtctgttt
     5161 gccgcatatg aatttttgga atcgggtttg caaaaatgga acggggagaa ttggttttct
     5221 gaaatcaacg atcaattccc attcccgttc aacttgctgc cggacgcgtt aaactggaat
     5281 ctcgctatgt atgcggagct tttactgccc gtactgttgc ttttgggttt ggcaacgcgt
     5341 ctgtcggcat tagggctgat ggtcgttacc gccgtcgctt gggctgcggt tcacgccggt
     5401 tcgggttaca atgtctgcga caacggttat aaaatggctt taatttatat cgtggtatta
     5461 atcccgctgc ttttccaggg tgcgggcgga tggtcgctgg atacgctgct gaaaaaacgg
     5521 ttttgccccc aatgccgtct gaagcaagat tgattcagtc gtggaatctg actttaaaca
     5581 ttccaacctt atctcgttaa cttgatattc tgaaaaggaa atagaaatga acaaaaacat
     5641 tgctgccgca ctcgccggtg ctttatccct gtctttggcc gccggtgcag ttgctgccaa
     5701 caaaccggca agcaacgcaa caggcgttca taaatccgcc catggctctt gcggcgcgtc
     5761 caaatctgcc gaaggttcgt gcggcgcggc tggttctaaa gcaggcgaag gcaaatgcgg
     5821 cgagggcaaa tgcggtgcga ccgtaaaaaa aacccacaaa cacaccaaag catctaaagc
     5881 caaggccaaa tctgccgaag gcaaatgcgg cgaaggcaaa tgcggttcta aataatccca
     5941 ccccttcaaa ccaagccgcg tttttcagta aaatgcggct tttttaacgg caaacaaaga
     6001 ttttttaaca agcacatcat tcttttgtgc catccgaacc gggtaaaaat atgattcaac
     6061 acgcaggctt gggctaccgc cgcgacttgg cggaagactt tctctcgctt tccgaaaaca
     6121 gcccgatatg ctttatcgaa gccgcaccgg aaaactggct gaaaatgggc ggctgggcac
     6181 gcaaacagtt tgaccgtgtg gcggaacggc tgccgctggc gttgcacgga ttgtctatgt
     6241 cgctgggcgg gcaagcaccg ctggatactg atttgataga cggcatcaaa gaaatgatgc
     6301 gccgttacga ttgcacgttt ttctccgacc atttgagcta ctgccacgac ggcggtcatc
     6361 tttacgattt gttgccgctg ccctttaccg aggaaatggt gcatcatacg gcgcggcgta
     6421 tccgcgaagt gcaagaccgt ttgggctgcc gcatcgccgt ggaaaacacg tcctactatc
     6481 tgcattcccc gcttgccgag atgaacgagg tcgagttcct caacgcggtt gcacgtgagg
     6541 ccgattgcgg cattcatctg gacgtgaaca atatctacgt caacgccgtc aatcacggcc
     6601 tgttgtcgcc ggaggctttt ttgaaaaacg ttgacgcaga tcgcgtgtgc tatatccata
     6661 ttgccggaca tgacgtggaa acgccggaat tgttgattga tacacatggc gcggcagttt
     6721 tgccgactgt ttgggacttg ctcgaacttg cctatgccaa gctgccgacg attccgccca
     6781 ccctattgga acgcgatttt aatttcccgc cttttgccga actcgaagcc gaggtcgcca
     6841 aaattgccga ctatcaaacg cgtgccggaa aggaataccg ccgtgcagcc tgaaatctcc
     6901 gcccaatacc aacaccgttt cgcccaagcc atacgcgagg gtgaagccgc cgacggactg
     6961 ccgcaagagc gcctgaacgt ctatatccgc ctgatacgca acaatatcca cagctttatc
     7021 gaccgctgct ataccgaaac gcggcaatac tttgacagcg aagaatggag ccgtctgaaa
     7081 gaaggtttcg tccgcgacgc gcacgcccaa acgccctatt ttcaagaaat ccccggcgag
     7141 ttcctacaat attgccaaag cctgccgctt ttagacggca ttttggcact gatggatttt
     7201 gaatataccc aattgctggc agaagttgct caaattccgg atattcccga cattcattat
     7261 tcaaatgaca gcaaatacac accctcccct gcggccttta tccggcaata tcgatatgat
     7321 gttaccgatg atttgcatga agcggaaaca gccttgttaa tatggcgaaa cgccgaagat
     7381 gatgtgatgt accaaacatt ggacggcttc gatatgatgc tgctggagat aatgggttcc
     7441 tccgcgcttt cgtttgacac cctcgcccaa acccttgtcg aatttatgcc tgaggacgat
     7501 aattggaaaa atattttgct tgggaaatgg tcaggctgga ctgaacaaag gattatcatc
     7561 ccctccttgt ccgccatatc cgaaaatatg gaaggcaatt ccccgggcca aaaccatcta
     7621 tccgcataaa attaccttgt tcccgatact atgccgctac ccgacctgac cgatgccgaa
     7681 ttaatagagt cgcgtaaact gcttctgcat tttgcgcggc ttcagttgcc cgaccaccct
     7741 gatttggctg aagatttagt gcaggaaaca ttgctgtccg catacagcgc aggcgacagt
     7801 tttcaaggca gggcacttgt caacagctgg ctttttgcca tattgaaaaa caaaattatt
     7861 gacgcattac gtcaaatcgg aaggcagagg aaagtcttta ccgcactgga tgacgagcta
     7921 ctggatgaag catttgaaag ccatttttcc caaaacgggc attggacgcc ggaagggcag
     7981 ccgcaacatt ggaacactcc ggaaaaatca ttaaacaaca acgaattcca aaaaattctg
     8041 caaagctgcc tatacaacct gcctgaaaac accgcacggg tatttaccct gaaggaaata
     8101 ctcggttttt catccgacga aatacaacaa atgtgcggta tcagcacgtc caactaccac
     8161 accattatgc accgcgcccg agaatcattg cgccaatgcc tgcaaatcaa atggttcaac
     8221 caagaaaacc cgaagtaaac gttatgaaaa aatgccgcga tatcgccctg cttctttcca
     8281 aacatcagga ccgggaaacc accccgggcg agaagatttc catatacaca cacctgctgt
     8341 tctgtccgta ttgccgtgaa tataaaagac agcttcaaac catcaaaata tcactggcaa
     8401 aaacaaccag aacttcaaaa taaatgccgt ctgaaaaggc ttcagacggc ataagctgac
     8461 ggaaacaaat caaaccgatt tactgttatc ggcagttcat ccataataca cgcttcaaaa
     8521 gcagcatatt cctccatacg gaatatataa atacgcaaaa cacgaaggct gcatcaattt
     8581 gctttatttg ccttatttca cagacggcgc tacccctccc gcccaacccg ttctttctga
     8641 atgagcagat ttcaatgatt aaggaaaccc taatgcgccc aatcttccta tctttcgttt
     8701 tactccctat tttgataacc gcctgcagca caccggacaa gtctgcccga tgggaaaata
     8761 tcggcacaat ctcaaacggc aatattcata catatatcaa taaagacagc gtgagaaaaa
     8821 acggaaatct gatgattttc caagataaaa aagttgttac caatctaaaa caagaacgtt
     8881 ttgccaacac ccccgcatac aagactgcca ttgccgagtg ggaaatccac tgcaacaaca
     8941 aaacataccg cttaagttcg ctacagttgt ttgatacaaa aaacacggaa atttccacac
     9001 aaagctacac agcctcttcc ctccgcccga tgagcatcct gtccgggaca ttaaccgaaa
     9061 aacaatatga aaccgtatgc ggaaaaaaac tctgattgca acttatatac aaacttaccc
     9121 acaaacctta tcataaaaat gccgtctgaa atactgaaat atcagcattt cagacggcat
     9181 tttgccattc cctgaaaatt atccacaaag ttatccacat tattttttaa aaccggcttc
     9241 catccgaaat atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac
     9301 agatagtacg gcaaggcgag gcaacgccgt actggtttaa agttaatcca ctatataaac
     9361 tcgctataca atttcactat ccaaacgtaa attgttccat tgatacacaa aacttcttac
     9421 ccccataatt ttgataaagc atttcttaca ttcccggctc cgtcccgtaa ccaacacagc
     9481 ctctaaaaaa taatcgcttg ttttgacacc gatacactca tatagtggat taacaaaaat
     9541 caggacaagg cgacgaagcc gcagacagta cagatagtac ggcaaggcga ggcaacgctg
     9601 tactggttta aatttaatcc actatacaaa tacagaaact caagaaaata accttgtgta
     9661 ttgaccatct caagcaattc agaaaaatca agaaattttc tgaccgtaaa caaacgtttc
     9721 cctaaaaaaa cgatgtcttc aaaaatatcg accaaataat catcttcgcc gttttcattt
     9781 ttatgggtta aaacaacacg ggaaaaatct gttttcagat gcttgcccgc ttgattgttc
     9841 ggattattgt ccggaacgac aaaaccgtcc tcaaaatcaa agtagacgtt gcgtccttct
     9901 acctttatct ctgtgcaata acaatcatgc agagaaatac catccgaaaa atttaggggc
     9961 tgtactagat tagcagatat gttaccctcg
//
LOCUS       DAWFIE010000069         9818 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWFIE010000069 DAWFIE010000000
VERSION     DAWFIE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9818
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.069"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..390)
                     /locus_tag="WGQ71_001612"
     CDS             complement(<1..390)
                     /locus_tag="WGQ71_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0707590.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEADVWMDAVIWQMPSWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRHRVNPTEG
                     YGTGGLLQGKKHMLSLTWNAPIEAFTRE"
     gene            629..1528
                     /gene="crgA"
                     /locus_tag="WGQ71_001613"
     CDS             629..1528
                     /gene="crgA"
                     /locus_tag="WGQ71_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ0707591.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1755..4970)
                     /gene="carB"
                     /locus_tag="WGQ71_001614"
     CDS             complement(1755..4970)
                     /gene="carB"
                     /locus_tag="WGQ71_001614"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ0707592.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFKLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQVLGYGICATRGTA
                     QYLTEQGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(5010..5621)
                     /locus_tag="WGQ71_001615"
     CDS             complement(5010..5621)
                     /locus_tag="WGQ71_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707593.1"
                     /translation="MINIIATWAMALTYIFLLLFIIVFIPIQLYLENIKKKKFKSRII
                     DILKNNHNNLDLNDIKQMTEAVNLNNFAARKIIKQLYYTDELNLNLVRQLQKEIQQEE
                     PFDGCSDELKPTLIGINELLETHGSESQKHLLTPIISELKELNQIKHDHKKMKTQSYI
                     AYIIAIISFFIGSISFYYTITAPSGKEIANTVVEQLKANQNQQ"
     gene            complement(6469..6858)
                     /locus_tag="WGQ71_001616"
     CDS             complement(6469..6858)
                     /locus_tag="WGQ71_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ0707594.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7430..7555)
                     /locus_tag="WGQ71_001617"
                     /pseudo
     CDS             complement(7430..7555)
                     /locus_tag="WGQ71_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7601..8734)
                     /gene="carA"
                     /locus_tag="WGQ71_001618"
     CDS             complement(7601..8734)
                     /gene="carA"
                     /locus_tag="WGQ71_001618"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ0707595.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNQTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWALGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2190 a         2506 c         2558 g         2564 t
ORIGIN      
        1 ttcgcgggta aacgcctcaa tcggcgcatt ccaagtcagt gaaagcatat gttttttgcc
       61 ttgcaacaag ccgcctgtgc cgtagccctc agtcggattg acgcggtgtc tgccgtcgct
      121 ttggtagagt ttgccgtgtc cagcggttaa tacttcgtct atgtattttt tcactgtcca
      181 aggctcgtgc atccaccagc tcggcatctg ccaaatcaca gcatccatcc aaacgtctgc
      241 ctcaacatca tagccggcat caatcacggt ttcttgaaca ttgtgtccga gcgcggtcaa
      301 aacttctttc gcttttttgt gaagcgtgcg gtttaacccg ccgtgagaat gtccgaacgc
      361 cttgccgccg tctaataata aaatattcat ttgttacctc gtttgtgaat tgatgaatgt
      421 attgtgccgt tttactttat aatttaaaag tgaaaaaata agaaaacact tttgcgtcaa
      481 atgaaataat cagatgtttc aacacacggg atgacacata aagcgtctcc ctatgtgccg
      541 tcctgattcg gaaggggtta cacccctccc aaataaagtc tgatcctgcc gccctaaagg
      601 gcggggtttc aaccgaaaag gaaacacgat gaaaaccaat tcagaagaac tgaccgtatt
      661 tgttcaagtg gtggaaagcg gcagcttcag ccgtgcggcg gagcagttgg cgatggcaaa
      721 ttctgccgta agccgcatcg tcaaacggct ggaggaaaag ttgggtgtga acctgctcaa
      781 ccgcaccacg cggcaactca gtctgacgga agaaggcgca caatatttcc gccgcgcgca
      841 gagaatcctg caagaaatgg cagcggcgga aaccgaaatg ctggcagtgc acgaaatacc
      901 gcaaggcgtg ttgcgcgtgg attccgcgat gccgatggtg ctgcatctgc tggcgccgct
      961 ggcagcaaaa ttcaacgaac gctatccgca tatccgactt tcgctcgttt cttccgaagg
     1021 ctatatcaat ctgattgaac gcaaagtcga tattgcctta cgggccggag aattggacga
     1081 ttccgggctg cgtgcacgcc atctgtttga cagccgcttc cgcgtaatcg ccagtcctga
     1141 atacctggca aaacacggca cgccgcaatc tacagaagag cttgccggtc accaatgttt
     1201 aggcttcacc gaacccggtt ctctaaatac atgggcggtt ttagatgcgc agggaaatcc
     1261 ctataagatt tcaccgcact ttaccgccag cagcggtgaa atcttacgct cgttgtgcct
     1321 ttcaggttgc ggtattgctt gcttatcaga ttttttggtt gacaacgaca tcgctgaagg
     1381 aaagttaatt cccctgctcg ccgaacaaac ctccaataaa acgcacccct ttaatgctgt
     1441 ttattacagc gataaagccg tcaatctccg cttgcgcgta tttttggatt ttttagtgga
     1501 ggaactggga aacaatctct gtggataatt ctataaaaac ctgccataaa attgttttta
     1561 ctcacttaat aactttgaga aaaaataaat aagatgatgt atagccggcg gcaatggcag
     1621 tctatatggg gagcgtgata gggctgttcc ttatgtacgg cttgtgtgtg gcaacatcgg
     1681 ctttccaaat gacaaaatgc cgtctgacac cgtaaaaaaa ggtttcagac ggcattttca
     1741 acccgattca ggcatcagtt gcggtttttc aaacgcccgt gcaactcttg aacgctgtac
     1801 acgcccagat ggtctcggct tttcgcacct tcgctcatcg cttcgccgcc ggcagtggtg
     1861 gtgtattgcg gcacacgttg ctgcaatgcg ctttggcgga tgatgtggct gtcggacacg
     1921 gattgcggat cgctggaaac ggtattcacg accagtgcga tttcaccgtt tttcagtgcg
     1981 tcgccgatgt gcgggcggcc ttcggggact ttattgatgg tttgtacaat cagtccctgt
     2041 tcggtcaggt attgcgccgt gccgcgtgtg gcgcagatgc cgtagcctaa aacctggaag
     2101 tttttagcgg ttttgataac gcgttctttg tcttcttcgc gcacggagag gaagattttg
     2161 ccggtcgggt tgaggcgttc gcccgcaccg agttgggctt tgtagtaggc ttcgccgaag
     2221 cttgcgccca cgcccatcac ttcgccggtg gagcgcattt ccggtcccaa aatcgtgtct
     2281 acgcccggga atttgatgaa tgggaacacg gcttctttaa cggcatagaa atcggggaca
     2341 acttcttttt ccacgccttg ttctttcagg gaaatacctg ccatgcagcg tgcgccgact
     2401 ttggcgagcg gcacgccggt ggctttggag acgaagggca cggtacggct ggcgcgcggg
     2461 ttcacttcca acacgaacac cacgccgtcc tgcacggcaa actgcacgtt catcagaccg
     2521 accacgccca gcgcgtacgc catcgctttg gtttggcggc ggatttcgtc ttggatttct
     2581 tcgcttaagg agtagggcgg cagcgagcag ccggagtcgc cggagtggat gcccgcctgt
     2641 tcgacgtgct gcataatgcc gccgataacc acgtctttgc cgtctgaaac gcagtccaca
     2701 tcgacttcaa tcgcgttgtt gaggaagaaa tcgagcaaca cggggctgtc ttcggaaacc
     2761 tgcacggctt cgcgcatgta tttttgcaag gcttcggcag agtggacaat ctgcatggcg
     2821 cggccgccca aaacataaga cgggcgcacg accagcggat agccgatttc ttcggctttg
     2881 acgagtgctt cttcttcgtt gtgggcgatg cggttgggcg gttggcgcag acctaagtcg
     2941 ttcaacactt tttggaagcg ttcgcggtct tcggcggcgt cgatgctgtc ggcggatgtg
     3001 ccgatgatgt ttacgccgtt ttcaaccagc gcgttggcga gtttgagcgg ggtttgaccg
     3061 ccgtaatgca caatcacgcc ccacgggttt tcggtgcgga cgatttccaa cacgtcttcc
     3121 aatgtcagcg gctcgaaata gaggcggtcg ctggtgtcga agtcggtgga cacggtttct
     3181 gggttgcagt tcaccataat cgtttcaaag cccgattcgc gcagggcgag tgcggcgtga
     3241 acgcagcagt agtcaaactc gatgccctga ccgatgcggt tcgggccgcc gccgagaatc
     3301 atcacttttt tacggtcgga aggacgggat tcgcattctt cttcgtaagt ggagtaaaga
     3361 taggcggttt cggtggcgaa ctcggcggcg caggtgtcaa cgcgtttgta aaccggatgc
     3421 agcttcagcg cgtagcggtg ttcgcgcact tctttttcgc ttacgttcaa caattgtgcc
     3481 aaacgtttgt cggagaagcc tttgcgtttc agacggcgta gggcggcgaa atccaaatca
     3541 ctcaaaatgc cgtctgaaac cgctttttct tccttcatca agtcttcgat ttgcgccagg
     3601 aaccaagggt cgatggcgca gatttcgtgg atttcttcca gcgtgaagcc ggcgcggaac
     3661 gcgtctgcca caaacagcat acgttcgggg ccggggtttg ccagttcgcg gcggatttcc
     3721 gctttgtcgg agcttctcgg attgaagccg cacaagcctg tttccaagcc gcgcagggct
     3781 ttttggaagc tttcctgaat cgtgcggccc atcgccatca cttcgcccac ggatttcatc
     3841 tgcgtggtca ggcggtcgtc tgcggcaggg aatttttcaa acgcaaaacg cgggattttg
     3901 gttaccacat agtcgatgga cggctcgaat gacgcggggg ttttgccgcc ggtgatgtcg
     3961 ttgcgcaact cgtccagctt aaagccgacc gccagtttcg ccgccacctt cgcaatcggg
     4021 aagcccgtcg ctttggaagc cagcgcggac gaacggctca cgcgcgggtt catctcaatc
     4081 acaatcatct cgccgttttc agggttcacc gcaaactgca cgttcgagcc gccggtatcc
     4141 acgccgattt cgcgcaatac tgccaacgaa gcattacgca tgatttggta ttctttgtcc
     4201 gtcagcgttt gcgcaggcgc aaccgtaatc gagtcgcccg tatgcacgcc catcgggtcg
     4261 aagttttcaa tcgagcagat aatgatgcag ttgtcgttct tgtcgcgcac cacttccatc
     4321 tcgtactctt tccagccgag aacagactgc tcaatcagca gctcgtgcgt aggcgacgca
     4381 tcgaaaccgc gttcgcaaat cgccaaaaac tcatctttat tgtaggcaat gccgccgccc
     4441 gaaccgccca tcgtgaaaga aggacgaatc agggtaggga agccgacctg ttcctgcgcc
     4501 gccaaagctt cgttcattgt gtggcagaca aaagatttcg ggcaagacaa accgattttt
     4561 tccatcgcct ctttaaagcg gccgcggtct tccgccttgt caatcgcgtc ttccgtcgcg
     4621 ccaatcagct cgacattgta ttttgccagc acgccgttgc gtgccaaatc cagcgcacag
     4681 ttcagcgcgg tctgaccgcc catcgtgggc agaatcgcat caggccgctc cttggcgata
     4741 atcttctcca ccgtctgcca cataatcggc tcgatgtagg taacatccgc catttcaggg
     4801 tcggtcatga tggtggcggg gttggagttt accaaaatga ctttatagcc ttcttcacgc
     4861 aaggctttgc aggcctgtgc gcccgaatag tcaaattcgc aagcctgacc gataacgata
     4921 gggccggcgc cgatgataag gatggatttt aggtcggtac gtttgggcat gatgggtctt
     4981 tctatgaaat caaatgttta gctaaaaatt tattgctgat tttgattggc ttttaattgc
     5041 tctacgacag tattggcaat ctcttttcct gatggggctg taattgtgta ataaaagcta
     5101 atactgccta tgaagaaact gataatggct atgatatagg ctatataact ttgtgttttc
     5161 atttttttgt gatcatgttt gatctgattt aattctttta attcagaaat aattggagtt
     5221 aataaatgtt tttgactttc agatccatgc gtttccaaaa gttcatttat tcctattagt
     5281 gttggtttta gttcgtctga gcagccatca aaaggttctt cttgttggat ttccttttgt
     5341 aactgtctaa ctaaatttaa atttaattca tcagtgtaat atagttgctt aataattttt
     5401 ctggcagcaa aattattaag gtttactgct tctgtcattt gttttatatc attaagatct
     5461 aggttattgt gattattttt taatatatca attattctag atttaaattt ctttttcttt
     5521 atattctcta aatatagctg aattggaata aaaacaatta taaagagcaa aagaaatata
     5581 tatgttagtg ccatagccca tgtggcaatt atatttatca tttatcaata tcttccattt
     5641 ggtttatata ttgtctcata ataagcaagc cgtagctcgc atgcagggtg tgtgcggtac
     5701 gcacgcgttc tccattttcc ctgcaacccc aacccaccgt gtgcgtgcct tgcggcacac
     5761 accctaccga tgggttcaaa ggtcgtctga aaaaacgctc gcggcgtttg ttacatctgc
     5821 cttgcgataa aggctgcctg aaaaattccc gctgttgata tttcgggcat tctttctcaa
     5881 accgcctgca accgcaaaat ccgctgcaac ctgccccctc ccccgtgggg gagggctggg
     5941 gagatggaat tctccgaatt tcggcaacct tccccaacac cctagccgcc caaacacaag
     6001 ccttgcggct tgttgccctc tctctggctc tctcccacgg gagagaggac ggggcggctg
     6061 tagggctaag ggttctgcaa ggaaacaggt tgtgcaggtt gctttttgct tttcagacga
     6121 cctgaaatca acggtaaatc caagtcgctt ttcaaaccgc ctgcaaccgc aaaatctgct
     6181 gaaacctact ccctcccccg tgggggaggg ctggggagag ggcattctcc gaatttcggc
     6241 aacctttcct aacactctaa ccgccaagac acaagccttg tggcttgttg ccctctctct
     6301 agctctctcc cacgggagag aggatggggt ggctgttggg gctaaggttt ctgtaaacta
     6361 atcaggttat gcaggctgct ttttgttttt cagacggcct ttataaatgc tcaattcagt
     6421 cggctttatc ctattatttt caataatcca atcaaaatca gccatctgct atcgtgcagc
     6481 ttgcttttcc aattcttgca acacgagcag gatttctgtc agcacatcgt ttgtctgctg
     6541 taaaatctcg tgattccaaa accgcagtac ggtaaagccc aagctgttga gatatgccgt
     6601 ccgcgcgtgg tcgtatgcgg cttgttctgt gtgctgccct ccgtctgctt cgacaatcaa
     6661 cttgggcgtt acgcacataa aatcaacaat atagtttccc atcggctgct ggcggcggaa
     6721 tttatagccg ttcagacgac ctgcccgcag gtgctgccac aattttgctt ccgccccgct
     6781 catttcttgg cgcatggctt tggcgcgttg gcgcaagatg gggttgtcgg tggtcaagag
     6841 tttctcgtgc gggttcattt tgttgtcgtg ctgtttgctg ccctctctct aactctctcc
     6901 catgggagag aggacgtggt ggcggttggg attaagggtt ttgcaaggaa aacaggttgt
     6961 gcaggctgct ttttattttt cagatgacct gcaatcaacg gttgtaaatc caagttgctt
     7021 ttcaaaccgc ctgtaaccgc aaaacttgct gcaacctacc ccctcccccg tgggggaggg
     7081 ctggggagag ggcattctcc gaatcgcggc aatctttccc taattcccta actgcctcaa
     7141 tacaagcctt tcggcttgtt gccctctctc taactctctc ccacgggaga gaggacggtg
     7201 cggctgtagt gattaagggt tttgcaagga aaacaaggaa aacaagttgt acaggctgtt
     7261 ttttattttt caagcgacct gaaatcaacg cttgtaaatc caagtcgctt ttcaaactgc
     7321 ctgcaaccgc aaaatctgct gcaacctacc ccctcccccg tgggggaggg ctggggagag
     7381 ggcattttcc aagttgcggc aatctttccc aaatccctta gtcttctaaa atacaagcct
     7441 tacggttgtt gccaaatgaa acttcttcca tacccaaaac ttgcgtgtaa aacgcaatgg
     7501 ttcggtcaat gtcggcaacg gtcagtacca gatggtcgag tgcgctaatc ttcatttccc
     7561 gctttctttt tcaggcggcg gcagtgccgt ctgaaagcca ttatgcccgt tttgccgctt
     7621 tcatattgcc aatgaatttg tcaaacaaat agccgacatc ttgcgggccg gggctggctt
     7681 cggggtggcc ttggaagcag aacacaggtt tgtcggtcag ctcgatgcct tgcaaagtgt
     7741 tgtcaaacaa ggatttgtgg gtaatgcgtg cgttagcggg cagggtgtcg gcatcaacgg
     7801 caaaaccgtg gttttggctg gtgatgacga ctttgccgct gtccaaatct tgcacagggt
     7861 ggttcgcacc gtggtggctg aagcgcattt tcagggtttt cgcgccgata gcgaggctga
     7921 tgagctggtg tcccaagcag atgccgaaaa tcggtttgcc gctttccatc agtttttgca
     7981 cggcttcgat ggcgtaggtg caaggctcgg ggtcgccggg gccgttggac aggaacacgc
     8041 cgtcgggatt gagtgccaac acgtcttgtg cgctcgtttg ggcggggacg acggtcaggc
     8101 ggcagccgcg cgaggcgagc atacgcagga tgttggtttt cacgccgaaa tcgtaggcga
     8161 cgacgtggta aggctgttcg tcaggggtaa cgaaaccctt gcccaatgcc cattcgcctt
     8221 ccgtccattc gtaagtttcc gtgcaggaaa cttcttttgc caagtctttg ccgaccatgc
     8281 tgccgaacgc ggcgatgagt tcttgcgctt tttcgattgt ggcatccgca ccggtcagaa
     8341 tcgcgccgcc ttgcgcgcct ttttcgcgca acagcgtggt caggcggcgg gtatcgatgt
     8401 cggcgatggc gacggtttgg ttgcgtacca aatagtcgtg caggctttcg gaggcgcgga
     8461 agttgctgtg caagagcggc aggtcgcgga taatcaaacc ggcggcgtaa acgctgcggc
     8521 tttcttcatc ttcggcgttg gtaccggtat tgccgatgtg tgggtaggtg agggtaacga
     8581 tttgtttgca gtaggacggg tcggtcagga tttcctgata gccagtcatc gaagtattga
     8641 acacgacttc gccggaagtc gaaccttcgt aaccgattga tgtgccgtgg aatacgctgc
     8701 cgtcagcgag gacgaggagg gcgggggtgc tcatgatggg aatcctgttt ttaataaaat
     8761 tcttgacaat gccgtctgaa gggacttcgg cagggggcat ccggtcggaa aaatccggtc
     8821 aaaaaaaaac acgccgcgca agatagacgc gtaacgtgct tttcgggcga tatgaaccct
     8881 gaaagcaacg tattttaaga cgaaacctac ccgatttcaa gcgcaaacgc ggcaggcggg
     8941 ctgatatgtt cgcgcgaaaa cggaaatccg tggcgtaaaa ccgcccgctc tgcgcccact
     9001 atgcccgtta cgcatttccc cacgtccgtc cggcgaaact atgggaatac ccgaatcgtc
     9061 aggaatacct gaatcgtcag gaatacctga atcgtcagga atacctgaat cgtcaggaat
     9121 acccgaatcg tcaggaatac ccgaaccgtc agaaataccc gaaccgtcat tcccgcgcag
     9181 gcgggaatcc ggacttgtcg gcacggaaac ttatcgggta caacggtttc ttgagatttt
     9241 acgtcctaga ttcccgcctg cgcgggaatg acggcatcgg tttgacggta tttaattgaa
     9301 ttgcggaaat tgacggattc agtgagattg gcgggatgaa gcctacccta tagcccgcct
     9361 tttatagcgg attaacaaaa accggtacgg gttgccccgc cccggctcaa agggaacgat
     9421 tccctaaggc gcccaagcac caggcgaacc ggttccgtac catttgtact gcctgcggcc
     9481 cgccgccttg tcctgatttt tgttaatccg ctatacgaac ccgccccctc ccgaaaaacg
     9541 caaaaaatgc cgtccgaaaa cctttcggac ggcattttcg cgtgaaccgt cattcccacg
     9601 aaaacagaaa accaaaatca gaaacctaaa attcgtcatt cccacgaaag tgggaatcca
     9661 gttttttgag tttcagtcat tcccgataaa ttgccttagc attgaatgtc tggattcccg
     9721 cctgcgcggg aatgacggga tttgagattg cggcatttat cgggagcaac agaagccgct
     9781 ctgccgtcat tcccacgaaa gtgggaatct agtttttt
//
LOCUS       DAWFIE010000070         9598 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWFIE010000070 DAWFIE010000000
VERSION     DAWFIE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.070"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            2..202
                     /locus_tag="WGQ71_001619"
     CDS             2..202
                     /locus_tag="WGQ71_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707596.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
     gene            370..905
                     /locus_tag="WGQ71_001620"
                     /pseudo
     CDS             370..905
                     /locus_tag="WGQ71_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            938..1129
                     /locus_tag="WGQ71_001621"
     CDS             938..1129
                     /locus_tag="WGQ71_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707597.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKIAAEIDSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            1301..1843
                     /locus_tag="WGQ71_001622"
     CDS             1301..1843
                     /locus_tag="WGQ71_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0707598.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVEQIRQARAE"
     gene            1937..3232
                     /gene="hisS"
                     /locus_tag="WGQ71_001623"
     CDS             1937..3232
                     /gene="hisS"
                     /locus_tag="WGQ71_001623"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ0707599.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRTYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLFLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRTQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAADLTNTLQQWKNA"
     gene            3233..3862
                     /locus_tag="WGQ71_001624"
     CDS             3233..3862
                     /locus_tag="WGQ71_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ0707600.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RATSQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISTAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            4016..5473
                     /gene="der"
                     /locus_tag="WGQ71_001625"
     CDS             4016..5473
                     /gene="der"
                     /locus_tag="WGQ71_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ0707601.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            5504..6403
                     /locus_tag="WGQ71_001626"
     CDS             5504..6403
                     /locus_tag="WGQ71_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ0707602.1"
                     /translation="MWFKQISFYPLNKEKLPEADALADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            6455..6871
                     /locus_tag="WGQ71_001627"
     CDS             6455..6871
                     /locus_tag="WGQ71_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707603.1"
                     /translation="MLKHLAFLLPAMMFALPTSAAVLTSYQEPGCTYDGGVGKDGLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVSANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIQEVKLPKNK"
     gene            6937..7503
                     /locus_tag="WGQ71_001628"
     CDS             6937..7503
                     /locus_tag="WGQ71_001628"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ0707604.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICKTSYKDRNGKYMGQTGVTLPKA"
     gene            complement(7567..8271)
                     /locus_tag="WGQ71_001629"
     CDS             complement(7567..8271)
                     /locus_tag="WGQ71_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ0707605.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNSFNRKLKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQTDASLEYTDFSVSRERRNEVIDQVSKDAILRFK
                     TRAEKLAGILGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     QGVEEISISVNGTVQF"
     gene            8417..9598
                     /locus_tag="WGQ71_001630"
                     /pseudo
     CDS             8417..9598
                     /locus_tag="WGQ71_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271049.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         2603 a         2432 c         2303 g         2260 t
ORIGIN      
        1 aatgcaattg atatgtgccg attggacagg tataggaaat tttatgaaaa ccttcgaaaa
       61 aacatggtct gcacaatatc gggatatgga aatttcagta cggaattttt ggaatttgga
      121 gcggacaggg gcggaagtct atatcaacgg aaggcgggtt tatcataacg aagccgaaat
      181 ggcgtctgct tctttgcgtt agctaatggg ggaatacctg gaatttgaag aaagcggtac
      241 gaaaattacc gttgaaatcg gcagcgcgtg gcattttaat gaaactataa gaaatattaa
      301 tacacattta agaggtacga aacttccagg ttctaactgg agagttcaag ataaggggca
      361 ttttcatctt tggaaaagat aatagattct aaggtaaggg agactaattt taatcaggca
      421 tggcgtgtta tacgcgccat gcaaatagat aatatatccg atgaacaggt tgtgattgtc
      481 agaatgtctt gttatcttga gaaagatgaa atttttaaga atgtagaaag atatggatat
      541 cagtcatcag atattaagaa aattagtcct ccttttccat taaatatttt acctgttccc
      601 aaaccttatt atttttatct ttatgatacc catttgaatc aaatttatga tttggcttgg
      661 tcatgtaatg attttttaat gtttgaaata tttattttgg atagggaaag catcaaatat
      721 atagataaag ataaactttt taaatctgga gaaacactat attcgaattt taaatctatt
      781 aaaaatcaat gcaaaaatta tttttttgcg gttttgattt tgatgcccct tactttaaaa
      841 gcggtgtgag tcaggtggca gattataatt ttgttcctga tgcattttct ttattcttta
      901 tttaatcaaa ttatttgaat aagcgtaggg tgggcacttg ctgcccacgc gttctgtttc
      961 aagtttatta ttgggtgaat atttggaatt tgaagaaaac ggtacaaaga ttgctgcgga
     1021 aatcgacagt gcatggcatt ttttgggcgc ggcttgtcgg atttcaataa atggaaagta
     1081 ttatgcgggt aatcggattg tttggtttgc caaaaaagcc gaaagctagg aataatttga
     1141 caagggcagc ctgaaagatt ttcaggctgc tctttatgtg gaaaatatga tgaagttcaa
     1201 atatgttttt ctgttggcgt gtgttgtcgt ttctttatct tatcgtttga atgctgcacc
     1261 gatgtttaac gataatcctg ttgtttacgg aaaaatcaaa gtgcagagtt ggaaagaaag
     1321 gcgcgatttc aatattgtaa agcaggattt ggatttttcc tgcggggcgg cttcggtggc
     1381 gacgcttttg aacaattttt acgggcaaac gctgacggaa gaagaagtgt tgaaaaagct
     1441 ggataaggag cagatgcgcg cgtcgtttga ggatatgcgg cgcattatgc ctgatttggg
     1501 ttttgaagcg aaaggctatg ccctgtcttt cgagcagctc gcgcagttga aaatccccgt
     1561 catcgtgtat ctgaaatacc gcaaggatga tcatttctcg gtattgcgcg ggatagacgg
     1621 caatacggtt ttgcttgccg acccgtcgct ggggcatgtt tcgatgagca gggcgcagtt
     1681 tttggatgct tggcaaaccc gtgagggaaa tttggcaggt aagattttgg cggtcgtgcc
     1741 gaaaaaagcc gagacaattt caaataaatt gtttttcaca catcatccca agcggcagac
     1801 ggagtttgca gtcgaacaaa tcaggcaagc acgtgcagag taagtcaaat gccgtccgca
     1861 aaccaccccc aacctcacaa acccttaaaa atccgctata atcgcccgca atttgattta
     1921 agcattctta cttgaaatgg cacaaaaaat ccaatccgtc aaaggcatga acgaccttct
     1981 gcctgtcgag caaaaagatt tcaaactgac ggctgcgttt tggcaggcgt ttgaagatac
     2041 ggtcggccgc tggacacgca cttacggtta tcagcaaatc cgtacgccga ttgtcgagca
     2101 aaccggtttg tttgtccgct ccatcggcga ggaaaccgat gtggtcggca aggaaatgta
     2161 caccttctcc gattcaaacg attctttgag tttgagcttg cgtcctgaag gtacggcttc
     2221 ctgcctgcgt gcggtggtcg aacacaacct tctctacaac agcccgcaaa agctgtggta
     2281 tatggggccg atgttccgcc gcgagcgtcc gcaaaaaggc cgttatcgtc agttccatca
     2341 ggtcggtatc gaagctttgg gttttgaagg tccggatatt gatgcggaaa tcatcgcgat
     2401 gtctgccgac ttgtgggaaa aattgggtat tcgcgaatac ctgactttgg aaatcaacag
     2461 cttgggcaac cgtgaggaac gcgcggcaca ccgtgcggca ttggttgaat atctgacccg
     2521 ttatgaagat aaattggatg aagacagcaa acgccgtctg aaaaccaatc ctttgcgcgt
     2581 tttagatacg aaaaaccctg atttgcagga aatctgcaat gccgcgccgc gtttggtgga
     2641 ttacttgggc gaggcttcgc aaaaccacta tgcacgcttc aaggcgatgt tggatggttt
     2701 gggtattcaa tatattgaaa atccgcgctt ggttcgcggt ttggattatt acaatcagac
     2761 ggtttttgag tggacgaccg acaaactcgg cgcgcaggcg actgtgtgtg gcggcggccg
     2821 ttacgatggt ttgattgaag aacttggcgg caaacctgcg ccgtctatcg gctttgcaat
     2881 gggcatcgaa cggttgctgt ttttggtgag cgaatacggc tcgctggaag tgaatgctgc
     2941 gcctgatgtc tatgcaatgc accaaggcga aagggcggac ttgcaagtga tgaaatacgc
     3001 gcaagccttg cgcacacaag gtttcaatgt aatgcagcat tccggctatc aaagcctgaa
     3061 agcgcaaatg aaaaaagccg acaacagcgg cgcgcgcttt gccctgattg tcgcgcaaga
     3121 cgaactggcg aacggtacgg ttacgctcaa agatatgaac ggcgcacacg gtcagcaaac
     3181 tgtcgccgcc gctgatttga caaacacttt acaacaatgg aagaacgcat aaatggcagc
     3241 ccatttggaa gaacaacaag agttggacaa ctttaaatat ttttggaaaa ccacgggcaa
     3301 atggctgttt gccctgctga ttttggcggc gctcggctac ttgggataca cggtttacca
     3361 aaaccgtgcg acttcccaaa atcaggaagc ggcggcggtg ctggcaaaca tcgtggaaaa
     3421 ggcgcaaaac aaagccccgc aaagcgaaat caatgccgaa ttgaccaaac tccagcaaag
     3481 ctacccgcat tccatttcca ccgcccaagc cacgctgatg gcggcggcaa ccgaatttga
     3541 cgcacagcgt tacgatgttg ccgaaggcca tttgaaatgg gtgttgtcca accaaaaaga
     3601 cagcctgatt caagcgttgg cggcgcagcg tctgggcgtt gtgttgttgc aacaaaaaaa
     3661 atacgatgcc gcgcttgccg cgctcgatac gccggttgaa gcggacttcg cccccctgct
     3721 gatggaaacc aaaggcgatg tctatgccgc acagggaaaa agccaggaag ccttaaaaaa
     3781 ctacggacag gctttagaaa aaatgcctca agattctgtc ggtcgcgaat tggttcaaat
     3841 gaaacttgat tcgctgaaat aaatgccgtc tgaaatgctg tccattattt atcagacggc
     3901 attttaccct ccctctttat ggcctcttgc cgtgaagaac ctatttaccg tgtccaacgg
     3961 acacctgctc gtgttcagtt ctcccgaacg gcacttgaca cagaaagacc caataatgaa
     4021 acccaccatc gcgcttgtcg gccgccctaa cgtcggcaaa tctactttat tcaaccgttt
     4081 gacgcgcacc aaagacgcac tcgtacacga cctgcccggc ctgacccgcg accgccatta
     4141 cggacacggc aaagtcggca gcaaacctta tttggtcatc gataccggag gtttcgagcc
     4201 ggttgtggac agcggcattt tgcacgaaat ggcaaaacaa accttgcagg ctgtcgatga
     4261 agctgatgcg gttgtgtttt tggtggacgg ccgtaccggc ctgaccccgc aagacaaaat
     4321 cattgccgac cgcctgcgcc aaagcccgcg ccctgtttat ctggcggtaa acaaaggcga
     4381 aggcggcaac agggccgtgc ttgccgccga gttctacgaa cttgctttgg gcgaccctta
     4441 tgttatttca ggtgcgcacg gcgatggcgt gtattatctg attgaagata ttttggaaac
     4501 cttcccagag ccggaagccg aagaagccga tgcaaaacat cccgtctttg ccgtcatcgg
     4561 ccgtccgaac gtcggcaaat ccacactggt taacgccatc ctcggcgaag agcgcgtgat
     4621 tgctttcgat atggcaggca cgacacgcga cagcatccac atcgatttcg aacgcgaagg
     4681 caaaccgttt accatcatcg ataccgcagg cgtgcgccgt cgcggcaaag tggatgaagc
     4741 agtagaaaaa ttctccgtta tcaaagcgat gcaggcggtt gaagcggcaa acgtcgctgt
     4801 tttggtattg gacgcgcagc aggacatcgc cgaccaagat gcgacgattg caggtttcgc
     4861 tttggaggca ggccgtgctt tggtggtggc ggtcaataaa tgggacggca tcagcgaaga
     4921 acgtcgcgag caagtgaaac gcgatatcaa ccgcaaactg tatttccttg attttgctaa
     4981 attccacttt atttccgcgt tgaaagaacg cggcatagac ggattgttcg acagcattca
     5041 ggctgcctac aacgcggcaa tgattaagat gccgacgccg aaaatcacgc gcgtactgca
     5101 aagcgcgatc gaacgtcagc aaccgccgcg tgccggcttg gtgcgtccga aaatgcgtta
     5161 tgcccaccaa ggcggcatga atccgccggt gattgtggta cacggcaatt cgctgcacgc
     5221 gatttccgac agctatacgc gctacctgac ccaaacgttc cgcaaagcct tcaatctgca
     5281 aggcacgccg ctcagaattc aatacaatgt ttcagaaaac ccctatgaaa atgcggaaga
     5341 caaaccgaag aaaaaaccgc tgcgccgcgt cagcctgagc aaccgtattg agaaacgcga
     5401 aggccgcaaa gaagagaaaa accgcttcaa gaagaaaacc aaagtcagtg tgaaaaaaca
     5461 attcagcaaa taattcccga catttcaaac aataggaaac attatgtggt tcaagcagat
     5521 tagtttttat ccgctcaaca aagaaaagct gcctgaggcg gacgcacttg ccgacaaact
     5581 tgctgaagct gaatttaccc attgccaagg cttagactgg ttcagcgaag gctttaccgc
     5641 accggtttca ttctccccgg aactcgtttt ccctgccgac tttaccttgc gcgtcgccct
     5701 gaaaaaagaa gaaaaagtcc tgcctgccgg cgtcatccgc gatattttgg aagagaaggt
     5761 agcggaaatc caaaacaatg aagcccgcaa tgtcggtcgc aaagaaaaac aagagcttaa
     5821 agagcaaatt acagacgacc tgctgccccg agcgtttacc cgcagcagcc gtacagaagc
     5881 ggtgtttaac acccgccacg gctacctgct cgtcaataac gcggcttccg ccaaagcaga
     5941 aaacatcctg accaagctgc gcgaagcttt gggcggtttg gaagcctcac tgccgaatac
     6001 caaacaatcg ccctcttccc tgatgaccgg ctggctgttg caagggcatt gcgaaggcgg
     6061 ttttgaattg gacagcgatt gcgaactcaa aggcacgggc gatattgttc ccgtcgtcaa
     6121 agtatccaaa caagatttaa ccgccgacga agtggttcaa cacgtcaaaa acggtaaaac
     6181 cgtaacccag ctcggtttgg tttggcgcga acaaatcgcc tttatcctca cgcaagactt
     6241 cacactcaag cgcatccaat acctcgacgt attgcaggaa gaagccgaaa gcaacggcga
     6301 cgatgccgcc ggtctcgcct tcgcctcgca aatcctgatg gcggaatccg tcagcatcat
     6361 gttggaagag ctggtttcct atttgggcgg ctggcaagat tgatttttca gacggctttt
     6421 ataccgtcag ccaaaatcca atggaataac ccttatgctt aaacatctcg cattcctact
     6481 gcccgccatg atgttcgccc tccccacttc ggccgccgtt ctgacttcct atcaagaacc
     6541 cggctgcacc tacgacggcg gagtcggaaa agacgggctt ccttcaggca aaggcatatg
     6601 gcgttgccgg gacgggcgcg gttataccgg ttcgtttaaa aatggcaaat tcgacggaca
     6661 aggcgtttat accgtttccg ccaaccgcga aatatttatc gaaccgttca attccgacag
     6721 taccaaattc cgcaacatgg tactctcggg cacgttcaaa aaaggcctgg cacacggcag
     6781 gtttaccgtc tcgcaaaacg gcgaaaccct cttcattatg aaatgtgaaa acggcatgat
     6841 tcaagaagtg aaactgccca aaaacaaata agccccgttt tcagacggca ttcccgaatg
     6901 ctttgtccga acaccgcaag aaacaaggaa aacattatga gcatcaagtc cgacaaatgg
     6961 atacgccgaa tgagcgaaga attcggcatg atcgaccctt tcgagccgaa ccaaatcaaa
     7021 gaagccgacg gcaaacgcat catctcctac ggtacgtcca gctacggcta cgacatccgc
     7081 tgcgcaaacg aatttaaaat cttcaccaac atcaacagca ccatcgtcga tcccaaaaac
     7141 ttcgacccga aaaacttcgt taccgttgaa gacgactgct gcatcatccc gcccaattcc
     7201 ttcgcactgg cgcgcacggt cgaatatttc cgcatcccgc gcaacgtcct gaccgtctgc
     7261 ttgggcaaat ccacctacgc ccgctgcggc atcatcgtca acgttacccc gttcgagccg
     7321 gaatgggaag gctacgttac cctcgagttt tccaacacca cccccctgcc cgccaaaatc
     7381 tacgccggcg aaggcgtggc gcaagtcctc ttcttcgaga gcgacgaaat ctgcaaaacc
     7441 tcatacaaag accgcaacgg caaatatatg gggcaaaccg gcgttaccct gcctaaagcc
     7501 tgaaatataa atgccgtctg aaccgtatca tccggtttca gacggcatct acctgtttat
     7561 ccgtggttag aactgaaccg tcccattgac gctgatgctg atttcctcca caccctgcgc
     7621 ggcagaatcc gcaccctcca tattgacgct tgccgccatc ggcatggcac gaagcatttt
     7681 tgcctgagca gctccccctc ccgcgatatg gctgccgatg tgtcccaaat ttaatttgac
     7741 gattttataa ccggacgcac ccaaaatgcc cgccagtttt tcggcacgcg tcttaaaacg
     7801 caaaatagca tccttgctga cctgatcgat gacctcgttt cggcgttcac gcgacacgct
     7861 gaaatcggta tattcaagcg aagcatccgt ctgaacatca gcaataaaac ggttcaacgc
     7921 atcaaaatcc ctaccttcga ccttaaattc cgcacgctcc tcccagcctg tttgaatgcg
     7981 tctgccgttg gtatattgat agcgcggcat cgcactgcgc gataccaatt cggttttaaa
     8041 gctgccgttt ttcaattttc tgttaaaact gttgaatttt ttaacaaact cggcattgac
     8101 ggcatttttg tcccgtcctt ccgccgccac ttggaaacgt gcggacattg tatcctgagc
     8161 cacctcgaca cccgccgatt cggaaaattc gacaatattg taattcaatg cttcagccgc
     8221 tgccggaaaa gataccgcca atagggaagc cgccaaaata gaacgcaaca tatccgtatc
     8281 ctcactcata aaaccgccaa tctagcatat cggcgcgcta aattgcatta gcgtaaggca
     8341 acaatttaaa tattcggtct gcgtctgtct tataataacc gcctttccga tacggaatcc
     8401 cactatgccc aaacccttgc acgtcctcgt catcccctca tggtatccgc aatccgaaca
     8461 ggatgtggac gggatttttt tcaaaatcag gcactggcat tgcagaggaa aggcatcaaa
     8521 accgccgtgc ttgcaccgat gttccgctac ttgcggaaag aaacagcaag catcctgacc
     8581 gaccttacgg ttttgcaaaa tatcggcaaa gcggtttgga catctatgca tggcacggca
     8641 tgtatttctt cccccgcttt ccgttcatcg acatcgaccg catccgctgg gtgcgcgtgg
     8701 gtttgaaagc cttcaaacac tacatccgcg aaaacgggct gcccgacctg attcacgccc
     8761 accgcatgaa ctatgccggc atactcgccc aaaaaatttc ccaaaaatac ggcatcccct
     8821 atgtcgtcac gaaacacagc agcaccatta cgcgcggtct gatccgtccc caccaatggc
     8881 agcctatgaa aaatgcggcg gcgcacgccg ccgcacgtct cgccatcagc cgccatttcg
     8941 cacacgtcct gcaacacaaa tacggctgcg aatggcagta cctccccaac atactgggcg
     9001 gaatattcaa ccgaccgttt gaacaaagag aaaaaaacaa caaaccgcat ttcgtgttct
     9061 gcaccgtctc gcaccttcgc cgtctcaaag gacacgatgt cctgctcact gcctttgccc
     9121 gggcgttggc acaatgcccg caactgcgtc taaacatcgg cggcagcgga caggaagaac
     9181 agcggctgaa acagcaggcg gcagacttgg gcattaccca tgccgttaca tttttaggcg
     9241 cactgcaacc cgaagcagtc ttggatttga tgaagaacag cgacgcattt gtccttgcca
     9301 gccgcaccga aaccttcggc gtggtctata tcgaagcact gtcccaagga ttgcccgtca
     9361 ttgcgacacg ctgcggcggt gcggaatcta ttgtttcaga cggcaacgga tatttggttc
     9421 ctgttgacga cgacgatgcc cttgccgacg cactcatcaa aatgtatgaa caccactctg
     9481 attttgaacc tgcccgactt agggaaaact gtctgaacga atttggcgaa aatgccgtta
     9541 taggcaggtt gatcggcatt ttccgacagg caatcgcaga atacggtaag aaaatacc
//
LOCUS       DAWFIE010000071         9581 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWFIE010000071 DAWFIE010000000
VERSION     DAWFIE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.071"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(100..297)
                     /locus_tag="WGQ71_001631"
     CDS             complement(100..297)
                     /locus_tag="WGQ71_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707606.1"
                     /translation="MTAMPSEPPFRRHFTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGITFLNHPF"
     gene            complement(294..1349)
                     /locus_tag="WGQ71_001632"
     CDS             complement(294..1349)
                     /locus_tag="WGQ71_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0707607.1"
                     /translation="MKTYAPALYTAALLTACSPTADSNHPSGQNAPANTESDGKNITL
                     LNASYDLARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSIVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            complement(1455..1937)
                     /locus_tag="WGQ71_001633"
     CDS             complement(1455..1937)
                     /locus_tag="WGQ71_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0707608.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            complement(1984..3120)
                     /locus_tag="WGQ71_001634"
     CDS             complement(1984..3120)
                     /locus_tag="WGQ71_001634"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ0707609.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQVVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTLDELKAAFTEHGGVDCVLEKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQMAQRLADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            complement(3117..3662)
                     /locus_tag="WGQ71_001635"
     CDS             complement(3117..3662)
                     /locus_tag="WGQ71_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ0707610.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRSAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            complement(3659..5134)
                     /locus_tag="WGQ71_001636"
     CDS             complement(3659..5134)
                     /locus_tag="WGQ71_001636"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ0707611.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVDKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGITNMNILAAT
                     FPAGTLSGAPKIRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            complement(5245..5610)
                     /locus_tag="WGQ71_001637"
     CDS             complement(5245..5610)
                     /locus_tag="WGQ71_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707612.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            complement(5631..7541)
                     /locus_tag="WGQ71_001638"
     CDS             complement(5631..7541)
                     /locus_tag="WGQ71_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0707613.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELSLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLAVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGGKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSL
                     VFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            complement(7560..8204)
                     /locus_tag="WGQ71_001639"
     CDS             complement(7560..8204)
                     /locus_tag="WGQ71_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0707614.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(8369..9187)
                     /gene="opc"
                     /locus_tag="WGQ71_001640"
                     /gene_synonym="opcA"
     CDS             complement(8369..9187)
                     /gene="opc"
                     /locus_tag="WGQ71_001640"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ0707615.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWSAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTSLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT         2123 a         2116 c         2677 g         2665 t
ORIGIN      
        1 cgctgtactg gtttttgtta atccactata aatgttccga tacgaactgc aaaatattgg
       61 ttttgtttct gacaggcaaa agcactgttt atttggctgt caaaagggat ggttaaggaa
      121 agttatgccc cagataagga tggttgcgcc gacggcttca gacggcattt tggcggcggt
      181 gttgggtttt gtatccggtt tgccgttgtg tcttgtgatg atgattttgg gcgcggtttt
      241 tctgttttga tgtgtgaaat gccgtctgaa aggcggttca gacggcatag cggtcatttt
      301 tgtgcggtca ggcggtcgaa tatgccgccg tcggcgaagt aggttttcat gatgttgtcc
      361 catccgccga atttttcttc gggagagaat gtgtctaagt ctgggaagtc ggctttgtgt
      421 cttgccaata cttcggggtt gcgggggcgc aggtagagtg aggcggcgag ttcttgcgcc
      481 ggttcgctcc aaaggtattc gagataggcg cgggcggttt tttgcgtgcc ttttttcgcg
      541 acgatgctgt tgacgacggc gacggggctt tcggcggaaa tggtgtagct cggatagacg
      601 atttcaaact gtccttgggt cagttttttg ctgacgtagt tggcttcgtt ttcaaaagtg
      661 atgagtacgt cgccgatgtt gcgttgtgtg aaggtggtgg tggcggcgcg tccgccgttt
      721 tcaaaaacgg gggtgttttt gaggatggat gcgacgagtt tttgggcttc ctgttcgttg
      781 ccgttggtgg ttttcagacc gtaaccgtat gcgccgagga aggcgtagcg tccgttgccc
      841 gaggttttgg gattggcgat gacgatgtta acgccgtctt tggcaaggtc gttccaatcg
      901 cggatctgtt tggggttgtt ttttcggaca aggaaaacca tagtgctggt gtagggcgcg
      961 gcgtggtcgg ggagggcttg ttgccagcct ttttctacca gtcctttttt ttcgagcagg
     1021 tcgatgtcgg aggattggtt catggttacg acatcggctt gaaggccgtt ggctacggat
     1081 aatgcctgtt tgctggagcc gccgtgggac tgttggatgc tgacggatgt gccggggtgt
     1141 tcggattggt atgttttgat aaataagggg ttgtattctt tgtaaaaatc ccgtgccaaa
     1201 tcgtatgagg cgttgagcag ggtaatgttt tttccgtcgg attcggtatt ggccggggca
     1261 ttttgtccgg acggatggtt tgaatcggct gtggggctgc aggcggtgag cagggctgcg
     1321 gtatagagtg ccggtgcgta ggttttcata tgcttgtcct gtcggttggt agatggggca
     1381 actttatacg gctgtctgcg cttgtggaaa taatgtttga tttgaagatt atcagttttg
     1441 gttataaggt gggattagag gtgtttgcgc atcagttcgc atttgatttt gatgctgggg
     1501 tcaagctgca atactgccga accgagcgat tcgtagcggt tgagaaggta aaacgggacg
     1561 gagttgagcg atgcgtagag tttcagaaag ctcaaaccgg atttgtgggc gatggtttcc
     1621 gcctgatgaa gtagggcagt gcccagtccg aggttgtgga acagggggtg gacgtaaagc
     1681 gcatcgagtt gtgcttcttg gcagtcgatt tggaaaaatc cctgtatgtt gcctttgtat
     1741 tcggcaaccc aaagtgcttt gtcgggatct gaaatggtcg gcaggtagct ttctgtgttt
     1801 aacaagcctt cccatacttt tagggcgtgt tcgttgtagc tgaggatgca ggtgtattgg
     1861 acggagtgca ggtggacttt gaagatgtct ttgcagtctt gcacggtggc ggggcgtaac
     1921 agtgtcagca ggttcatggc agtatgtcgg cggcttcaga cggcatctgt gccgttggtc
     1981 ggattatagg gactgatgca gtttttttgc ttcttgaaat gcggtgtccg aatcggtggt
     2041 taaaacggta aagtgtccca ttttccgccc tttgtgcgcg gtttttttgc cgtaaaggtg
     2101 caggtgcgcg tccggccggc tttgcagggg aaaccaatcc ggttcgccgc cgtcttcctg
     2161 ccaaacgtcg cccaaaatat tcgccataca gcaagaactc agcaatttgg tgtcagcagg
     2221 tggcaggttg cacatcaggc ggacctgttg ctggaattgg tctgccgcgc aggcatcgac
     2281 ggtatggtgg ccggagttgt gcggacgcgg cgcgatttcg ttgacgacca attcatgtgt
     2341 gtcaccgaca acaaacattt ctaccgccaa tacgccgaca taatccaatt cgtctgccaa
     2401 gcgttgcgcc atctgccgcg cctgttgctg cacgtcggca ctcagtcgcg cggggacgat
     2461 ggaataagcc aagatgccgt tttcgtggat gttttcggca gggtcgaaag tttgcacgtt
     2521 gtcattgttc aaacggcata caattacgga aatctcactg cgcaaatcca ccattttttc
     2581 caaaacgcaa tccacgccgc cgtgttcggt aaacgcggct ttgagttcat ccaatgtttt
     2641 tacgcggatt tgacctttgc cgtcgtagcc caacgtagcc gttttcagga tgccgggcaa
     2701 aaattgcgcg cttgcttcag tgatgtcttc agccttacaa accacttgat acggcgcggt
     2761 ttgcaatccc gctttgcgta tccatgcctt ttcctgaatg cggttttgtg caatcgccac
     2821 acaatcgccg ctaggggaaa cattggtatg ttttgccaaa aagcgcatcg catcggcatt
     2881 gacgttttca aattcagtgg tcaccgccgc gcattttgcc aattcatcca aagccgcttg
     2941 gtcgttaaac ggcgcgcata aatgacagtc ggcaaattct gctgccggcg cgtccggatc
     3001 ggggtcgaga acggttactt tgtagcccat ggttttggca gcaacagtaa acattctgcc
     3061 taattgtccg ccgccaagaa tgccgagcat ggcgggcgga gaaagagata tgtttttcat
     3121 gctgactctt caaattgtac aagttgatag ctataactaa ttcttgacgg atgtcttgta
     3181 tcgctggaat taccagtttc agaaatacag aatacttttt ccataaattt ttctgctttt
     3241 agaaattcca gtattctgtt tttttcatcc ttataagcac tgcggtctgt accccatgca
     3301 agaataatca tatcagcatc ttccaaacat cctttaaatt tggaaaaatc ggtttgggtg
     3361 tttgccctaa ttcctgtttg ctgtgtagag taactggaaa aaatatttaa cattttgaag
     3421 ttggtaaaac cgtacatatc caagaaacgt gcaagttggg taagggtttt gtcgcttctt
     3481 tcatcatttg ctttacttgg attaattcct atagcaacag ctgagaaatt tttaggattt
     3541 tctgtgttgc cgctccatct aaccgttagg atttctcgac ttttttcatt atctgtatag
     3601 agaccgtctt tggtagtcgg tctgagtgtt tggcgaagct cataaagttt ttcataagtc
     3661 atttatccaa cccttcctgc accatctgcg cggcgcgtat aacggctcgg gctttgtttt
     3721 gtgtttcctg ccattcggag gccggatcgg agtcggcaac cacgcccgcg ccgctttgga
     3781 cgtatagcgt gttgtttttt actacggcgg tgcggatggc gattgccaaa tccatgtcgt
     3841 tgttgaagcc ccatacgccg acggcgccgc cgtagatgcc gcgtttgctc ggttcgactt
     3901 cttcgatgat ttccatagcg cggattttgg gtgcacctga aagtgtgccg gcggggaagg
     3961 tggcggcgag gatgttcatg ttagtgatgc cgtctttcag acggccttcg acgttggaaa
     4021 cgatgtgcat cacatgggag tatttttcaa tcaccatttt gtcggttact ttgacttcgc
     4081 ccgttttgct gatgcggccg acatcgttgc gtcctaaatc gataagcatg acgtgttctg
     4141 cgatttcttt ggcgtcgctt aataagtctt gttcattggc aaggtcttcg gcgggggttt
     4201 tgccgcgcag gcgcgtgccg gcgatggggc ggacgatgac atcgtcgcgt tcgcggcgga
     4261 cgaggatttc aggcgaggag ccgacgatgt ggaaatcgcc gaaatcgtag taaaagaggt
     4321 aaggcgaagg attgagcgtg cgcaaggcgc ggtagagggc gagcgggctg tcggtgaatt
     4381 ccatgctcat gcgctggctg gggacaacct gcatgcagtc gcctgcaaag atgtagtctt
     4441 tgattttatc gacgcaggct ttgaacggct cttcgccaaa ttcgctgacg gcttcggtgt
     4501 gtttgctgcc gagcgagagc gggatggcgc agctttggcg caactgggtg cggatgtctt
     4561 cgaggcgttc gcgggcgcgt tcgtagccgt cgggctgcga cggatcggcg taaacgacga
     4621 gatggatttt gccgctcaaa ttgtcgatca ccgccaattc ttgcgacagc atcagcaaga
     4681 tgtcgggcgt gccgagcggg tcggctttgg tggtgttttt caggcggtgg gcgaagtgtt
     4741 caaaattgta gatggtttcg taaccgaagt agccgaccag tccgccggta aagcgcggca
     4801 ggcttgggat ttcgggtgtt ttgaagcggt tgtggaaggc ttcgataaag ggcagggggt
     4861 tgccgtcgtg ttgctcgaca atttcgccgt tttgataaac atcgacgtgt ttgccgctgg
     4921 ctttgagata gtggctgcaa ggcaggccga taaaagaata gcggccgaaa cgttcgccac
     4981 cgacaacgga ttcgagcagg taggtatagg ggcggttggc gagtttgaga tagagggaaa
     5041 gcggcgtatc caaatcggca aggagttctt gcacgagcgg gatgcggttg tagccttggg
     5101 cggcttgggc ttggtattct tgtttgctga tcatttctgc tttcccaaag ggcggtttcg
     5161 gacggcgcgg caacgggcgc gagtatagca ttttatcgga attgttgaca gtctgaccgg
     5221 agatgccgtc tgaaaaagac gggcttacgg ctttgttttg cgctgccggt attgtttcca
     5281 tttaaacaga taacctgcgg ttacgggaat cagggctaag atggttttga acgtccatga
     5341 tgcataccaa ggtaaagaac cgggtgcgac cgattgattc agatacggcc accaacaggc
     5401 agtcaggcag ccgagcagca gcggccagat atgccggctg aggttttttt gccaaaggag
     5461 gaatgatgcg gcagaccaaa gcagggtaag ggcagtgatt tttaacacgg tataagcgtc
     5521 cgctccaaac agggcggcga gaaaatacgt accggcaatc cagaggacgg caagtatcag
     5581 gacgatgatt tcttcaggtc gtttgaacat ttttttcttc ctgtttgatt tcagacggca
     5641 ttgccgttct gtttggtttc cagcagctcc cagcgttcca gcttttccaa aagcagcatt
     5701 tcgatttctt cggcgcggct ttgcagtgcg ccggcttttt cgtaatcttt gaagatttcg
     5761 ggatcggaaa gctgcgcgtt gatttctgcc tgttcggctt ccaaggcggc gatttcgtcg
     5821 ggcagggcat cgagttctcg ctgttctttg taggacagtt tgaccgtgcg gttggctttg
     5881 ggtttttctt tcaccggttc ggcatcggct gttttgggtg cggattccgt ctgaatcttc
     5941 gcttcgcgtg attttgcgtc gatgtagtcc tgatagccgc cgatatattc ttttaacctt
     6001 ccatttcctt caaaaacaag gctttgggta atcacgttat cgaggaacat acggtcgtgc
     6061 gagacaagga ataccgtgcc ttgataatcg cgcaacaggt cttcgagcag ctcttgggtg
     6121 tcgatgtcca agtcattggt cggttcgtcc aagaccaaaa tattggcggg acgggtaaag
     6181 agttttgcca gcaaaaggcg gttgcgttcg ccgccggaga gtgaagaaac aggactttgc
     6241 gcgcgggcag gggggaacag gaaatcttcc aaatagctca tcacatgctt tttcttaccg
     6301 ccgacttcaa cgtaatcatt gccctgtccg agcgtgtaaa acacagtgtc gttttcattc
     6361 aacgcgctgc ggaactggtc gaaataggcg acttcctgct tgctgccgat acggattctg
     6421 ccgtaggtcg gctgcaattc gcccaaaatc agcttgagga aggtggtttt gccgataccg
     6481 tttgggccga ttaagccgat tttgtcgcca cgctgcaaga tggcggagaa tttgtccata
     6541 atgactttgc cgccataggc aaacgaagcg tgttccaatt cggcgatgat tttgccgctt
     6601 ttctcaccgc tatcgagctt aaagttgacc tgtccctgca cgttgcggcg ttctgcacgt
     6661 tggcggcgca gctcttccaa acggcgcacg cgtccttcgt tacgggtacg gcgcgcttcg
     6721 atgcctttgc gtatccacgc ttcttcctga gcatgaaatt tgtcaaacaa gcggttatgt
     6781 tccgcctcga ctgccaactc ttgcgctttt ttctcactgt atttagagaa cgagccggga
     6841 taggaacgta gaatgccgcg gtcaagttcg acaatccgcg tagcgatatt atccaaaaaa
     6901 cggcggtcgt gggtaatcac ggccaagctg ccttcaaacg ccttgagcag gttttccaac
     6961 cagataatcg cgtcaatatc caaatggttg gtcggttcgt ccagcagcaa tacgtcgggc
     7021 ttctgcaccc aagcctgcgc caaggcgaca cgctttttct gaccgccgga aaggttgccg
     7081 attttttcat tttccggcaa actgagttcc cccaaagtct gcttgactgc cgcatccagc
     7141 ttccagccat ccttcgcttc gatttcaagt tgtaattcgt tgagttcttt caacaaagcc
     7201 tcactcgaac cattttccaa ctcatggctg acatgatgat aacggcgcaa taaatcacga
     7261 atttcgccca aaccttcggc aacggtatca aatacggttg cgtccttatc aaaaaaggat
     7321 tcctgcggta cataaacgat tttgaggttg ttttgaacaa taatctgccc gtcgtcgagc
     7381 ttttgcacac cggcgaggat ttttaaaaac gaagacttgc ctgcgccgtt gcgtccgatc
     7441 aagccgactt tttcgccgct gtcgagttga aaagaagttt tgtcgagcaa ggcaacgtgg
     7501 ccgatggcaa aagaagcgtt ttctacagat aatatattca tgatacaaat tctcaacagt
     7561 taccgtttgg attttaccgc aagtttggcg cgggcaattt caactgcacc cggcaggacg
     7621 gaaacaataa tgatgccgcc catcaccaag cccagattgt tttttacgac ggggaagttg
     7681 gcaaagaaat agcccgcgta agaaaacagg ataacccaca acaagccacc gatgatgttg
     7741 tagcggataa atttggcata gtgcattttc cccataccgg cgacgaaggg ggcgaaggtg
     7801 cggacgatgg gcataaaacg ggcaatgatg atggttttgc cgccgtgttt ttcgtaaaaa
     7861 cggtgggttt tatcgagata ttcacgtcgg aagattttag aatcggggtt ggcgaacagc
     7921 ctgccgccga aatatttgcc gacggtaaaa ttgagcgcgt cgccgagtat ggcggcaagg
     7981 cttaataatg caaccatcaa atgaatatcc ataccgccca gcgcggcaat cccgccggcg
     8041 gcaaacagca gcgaatcgcc gggcagtaag ggcgtaacaa tcaggccggt ttcgcaaaaa
     8101 acaatcaaaa acagaatcgc ataaatccac acaccgtatt gcgccgacag cgcgagcagg
     8161 tgttggtcga tatggaggat gaagtcgatg gcggaggcaa gcacggcgcg ttcctaaaaa
     8221 aaacaaaccg cgtattttaa ccgattggaa aaatgccgtc tgaaaagttt cagacggcat
     8281 cggctattca aattcatttc acgtaaaaac cgcaaaccaa aatagtttgc ggtttggcat
     8341 ttaaagtgac aatgatgatt tcaaatcatc agaattttat gccgacgcgc aagccgtatt
     8401 cacgaatact ggttttcggg atggtgagcg atacgtcgcc actcttggtt gttacactaa
     8461 actcgccgga ttctttgtaa gtgcgttgtt tgtagaacgg ccccgcctcg atgctggcgg
     8521 attcgcccag ttttttacca atatttgcac ccaatccaaa gccccagccg ttggtttttt
     8581 gattgatgtc ttttttgaga ctggtaacgc cggtgtttaa tttatagcgg gaattgaggt
     8641 caaatttcac ttcagaccaa gggttgatat accagccgtt acccagttgg gaaagcaaat
     8701 ccgcgtgaac tttggctgac cacgactgac ggctgctgtg aagcgtatgc ttggtggttt
     8761 taatgctgtc ttttgaagat tcaaaaccca agccggcacc cacacggaaa tttaaagaat
     8821 cacttaacgt ttgggtgtag gtgtagcctg tgtaaagatc gatacggttt tcaggaacgc
     8881 cggtgggcag ttttacattt ttagtcttac ccagcttgtt ctcatctgtt tccaaattaa
     8941 taatattttt tttgctgcgc ccgaaaccgg cttccaagcg gatgccttgg ttggcatcaa
     9001 aaggaatatc agcacgtacg ccgatgtgtt tggcagcttt gtgtttttct ttcaggaaag
     9061 cacgagttga agaaatggaa gagaggtcgg tgtggacggt aaactcatta gcggtttgaa
     9121 gctcttgtgc agcggcggca gtaccggtca gggcaatcat ggcacatgta aaaactgttt
     9181 ttttcatagt taaaatctcc aaaatttgga ttgtagtcgg atatggtaac ataacgtaaa
     9241 taatcgttac gcttacaatt atattcttaa gctttcgggg ggggggggga ttttacatat
     9301 attaataaaa attaacaaat agttatttgt ttacaacgaa ttgttattct cacttggttt
     9361 tctgtttttt atgggaatga cgaaatttta gtttgtgtgt atttatcgga aaaacagaaa
     9421 cccgccgccg tcattcccgc gcaggcggga atctagaacc caacgcgaca aaaatttatc
     9481 cgaagcgaca acaatctttt catcgtcatt cccgcgcagg cgggaatcta gaacgtaaaa
     9541 tctaaagaaa ccgttttacc cgataagttt ccgtgccgac a
//
LOCUS       DAWFIE010000072         9544 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWFIE010000072 DAWFIE010000000
VERSION     DAWFIE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.072"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(105..623)
                     /locus_tag="WGQ71_001641"
     CDS             complement(105..623)
                     /locus_tag="WGQ71_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ0707616.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDTILNQKECFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(638..865)
                     /locus_tag="WGQ71_001642"
     CDS             complement(638..865)
                     /locus_tag="WGQ71_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707617.1"
                     /translation="MKVYVFKISNENGKLKIELPEIPMGKQIDEVDLIAGLTTEFIAS
                     MLRDAQKDRRKFVIDASNQLAAIQTYQKIFN"
     gene            complement(929..1096)
                     /locus_tag="WGQ71_001643"
     CDS             complement(929..1096)
                     /locus_tag="WGQ71_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707618.1"
                     /translation="MNEKDLIEWLEDRGELMIMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            complement(1089..1262)
                     /locus_tag="WGQ71_001644"
     CDS             complement(1089..1262)
                     /locus_tag="WGQ71_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707619.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATKFMVKRNREQEVAEYQAQFEDKD
                     GKGRLKTEGNKDE"
     gene            complement(1371..1637)
                     /locus_tag="WGQ71_001645"
     CDS             complement(1371..1637)
                     /locus_tag="WGQ71_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707620.1"
                     /translation="MQVLKKVDWKMFVARSFWRWVPVGLTVGVWCFVAGMALHSCAKE
                     PEPVAKELTKVEKMERQADLEVKVWEAQYESMSVEEKMKGIVYE"
     gene            complement(1727..2641)
                     /locus_tag="WGQ71_001646"
     CDS             complement(1727..2641)
                     /locus_tag="WGQ71_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEZ0707621.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIDTSPASISMYLNGT
                     YADKGGNYETIETKIEAFLEMEDSKARREELVLGFVSTKTTRRIAEVMRDAHEGGETV
                     VIYGQAGLGKTQSVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSVALNLGESLPDDELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKMQAGIVKKYSSLIIR"
     gene            2712..2789
                     /locus_tag="WGQ71_001647"
                     /pseudo
     CDS             2712..2789
                     /locus_tag="WGQ71_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(2856..4844)
                     /locus_tag="WGQ71_001648"
     CDS             complement(2856..4844)
                     /locus_tag="WGQ71_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0707622.1"
                     /translation="MGITIGELLKLNIQGLPTTARGLRERAKTQNWPYIEEVGKARGG
                     RLKKYLIASLPAEIRAAIMKRQSDELTEKMPKTLPQVRPGTAMSAQALAEAAKLLNEK
                     QRSVADARCAVVAAVLGIKYQYGCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSR
                     SAKVGERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSA
                     PKLAHSYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPMIMQERGRKTGAAYKSLLPY
                     VKRDWGALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAE
                     SCVAVSDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNP
                     QGRGIIERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQ
                     QKYLKKLPSWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYTPKAYREMRLEQDGIAP
                     DMLSAEELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASAVN
                     VFDMDGKFITKAQANGNSREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALE
                     QAAVWDELGHLGGNVIEAEYAVLPRTGTDDFVLFEADR"
     gene            complement(4855..5043)
                     /locus_tag="WGQ71_001649"
     CDS             complement(4855..5043)
                     /locus_tag="WGQ71_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0707623.1"
                     /translation="MGIKTVNEVREWLKDNNLTVAQWARENGHNSVDVTRVLTGKSKC
                     RYGQGRAIAIKLGLRIDE"
     gene            5163..5525
                     /locus_tag="WGQ71_001650"
     CDS             5163..5525
                     /locus_tag="WGQ71_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003741237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEZ0707624.1"
                     /translation="MDNSFELSSKLLGERLKKIRTDNGYSREQLSELFGISRASIQNY
                     ENGERSPNADYLVQFYKHFGINLHWLLTGNKAAKFQDFIDSVSSPREEVLLHLARQMD
                     SRTLNHLIDFLMDVYAKK"
     gene            5801..6241
                     /locus_tag="WGQ71_001651"
     CDS             5801..6241
                     /locus_tag="WGQ71_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0707625.1"
                     /translation="MGNPVDLSEFVFVPRYNVAAAAGYGAPVFGEEPLFCLAFRKYWI
                     DNYVTKQPEKLSVITVKGDSMEGVLNHGDSILINHAKTDPRDGLYVLRIGNDLFVKRV
                     QRIPGKLLVTSENPRYAPFEIDFSNAQDDIAIIGRVEWYGRSID"
     gene            6393..7040
                     /locus_tag="WGQ71_001652"
     CDS             6393..7040
                     /locus_tag="WGQ71_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEZ0707626.1"
                     /translation="MSAFFTLFVPCAKTNSPPQENVALFRHNGEILLSGINIKLKSGD
                     SLLIRGPSGCGKTSLLRALAGLWPFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVC
                     YPDIDKQHPELIEAMNTCRLGYLIDKLDKTDDWQHKLSLGELQRVAFVRALLSKPKIV
                     LLDEATAALDEPAEAALYRALKQKLPDSIIISIGHRSTLNAFHNMRLDVGNVACG"
     gene            7145..7813
                     /gene="hda"
                     /locus_tag="WGQ71_001653"
     CDS             7145..7813
                     /gene="hda"
                     /locus_tag="WGQ71_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ0707627.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            7810..8478
                     /locus_tag="WGQ71_001654"
     CDS             7810..8478
                     /locus_tag="WGQ71_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0707628.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIISHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPICHLFGITNIIGTQLETDPDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLQSYGKTYFYSDSRNDLPLLRLVSEPVTVNPDAELEKEAKEKGWPV
                     LNFK"
BASE COUNT         2411 a         2659 c         1910 g         2564 t
ORIGIN      
        1 cctttaaatc atgggttaaa gcctatttga accggctagg caagctcctg ctccgtaggc
       61 tcaatcacgt ttcaacacac tgaattgaaa tcaggctgtt gattttatag aatttgttcg
      121 ctcggctcaa tcacaaaatc ctccacgccg ctcacaatct taatccccgg aacttggccg
      181 tctgaaaaac actctttttg attcaggatg gtgtctttgt cgatttcctt tttagtgcgg
      241 acaaactcgg caaaggcgga tttttccgag agccacgcca ggacggcggc cacgcccgtt
      301 accttgacag atggcggacg gatgcgccat ttaatcaggc cggtggtaaa gtccacggtt
      361 ttggttttac cattttccgt cagctcgtcc ttatgcgctt cgcagtaggc ggccacacgt
      421 tcggtcaggc tcatgatttc ggcacacatc ggcgcggctt tggcggcata ttcttcttcg
      481 atagccgctt ttttgtctcc ggcttcggtt tccaggcgtt tgacttcgcg ctgcaagtcg
      541 ccgatttggc ggataaatgc agtaacttcc gttttgtctt gtgccgcttc gatggcgggc
      601 tgtttgattc gggttttagc cattttctct tccttcctta attgaagatt ttttgatatg
      661 tctggattgc agccaattga tttgatgcgt cgattacgaa tttgcgacga tctttttggg
      721 catcacgtaa catacttgca ataaattccg tggttagccc agcaatcaaa tcaacttcgt
      781 caatttgctt acccattggg atttcgggga gttctatctt taacttaccg ttttcattac
      841 taattttaaa aacatacact ttcatttcac tttcctttct tgtttaaaat tgcttcaact
      901 ttcgcgcggt ttctcaaaaa cttaaacatc acacttcctc ccataaagtt atcgcctgag
      961 ccaaagtttc cgcctcggca gtcttccaca tcccgtccgg cgcacgcgct gcaatcacaa
     1021 agccttcgcc gtcctttttc ataatcatca gctcgccacg gtcttcgagc cattcgatca
     1081 aatctttttc attcatcttt gtttccttcc gttttcagac gacctttgcc gtccttatct
     1141 tcaaactgcg cctgatattc ggcgacttcc tgctcgcggt ttcgcttcac cataaacttc
     1201 gtcgcacgcc tgcggtgttg tccccaagcc tgccaatccg tattacgtcg tccgaaactc
     1261 atttcacaca tcctttcaca atcgccttat cgccatattt cgcgcggatt tcctttaccg
     1321 cccgatccaa agcctctttt ttcgccgcag ggctcaataa aattggctta tcactcataa
     1381 acaatccctt tcattttttc ctcgacgctc attgactcgt attgcgcttc ccataccttg
     1441 acctccaaat ccgcctgtct ttccatcttc tcgaccttcg tcagctcttt cgcaaccggt
     1501 tcaggttctt tcgcgcagga atgcaacgcc attcccgcca caaaacacca cacgcccacc
     1561 gtcaaaccaa ccggcaccca acgccaaaaa gagcgcgcca caaacatctt ccaatcaact
     1621 ttcttcaaaa cttgcatttt gcgttttcct tttaaaacaa caacttatta aaatcatagg
     1681 gtaaaaaaat atatagccct gtcagagact taccgtttca gacggcctat cggataatca
     1741 gggagctgta tttcttaacg ataccggcct gcatcttgat accgttcttg ttcgccgtgc
     1801 gtaccgcgcc gcgcatcaat ttactcatcc ggcgcgtatt gccgttacta tgtttaacca
     1861 gttccaagag cgtttcttcg tccgcatcag gcaaagccgc tttcgcaatc tcaaagagtt
     1921 cgtcatccgg caaagattcg cccaaattca gcgcaacaga cacgcggcta taaagttgca
     1981 ccagctcgcc atgcttaccg cgcaaattcg caaccagtcg gggcataccg ctcaacacca
     2041 aaccgcagcc cgtttcatca tgcagtcggc ggataatctc aagggcgcgc aagggcaggt
     2101 tttccgcttc atcgaccaca atcagacggc ccgaatcgcg caggcggtca gatacagact
     2161 caaacaaatc attcaggctg cccatcgccg ataccttcgc cgccgtcgcc aacttgcgca
     2221 tcagtaccaa agccgtaaag ctcggattag cctcaatcaa gatagccgca gggtttttct
     2281 cgcagtagtt tttgaccgac tgcgtcttgc ccaaacccgc ctgaccgtag atcaccaccg
     2341 tttcgccgcc ttcgtgcgca tcgcgcatca cttccgcgat tcggcgtgtc gtcttagtcg
     2401 atacaaatcc caacaccagc tcttcacgtc gcgccttact gtcctccatc tccaaaaacg
     2461 cctcgatttt cgtctcgatg gtttcataat tgccgccttt atcggcataa gtgccgttca
     2521 gatacatact gatggaggcc ggcgaagtat cgataccgcg tgccagttgg gtttggttca
     2581 ttcccgattt ttctttaaat tcaaccagtt tttgttgcaa tgcttgatta atttgtttca
     2641 tttttaatat ccttgagttt taaacaacct ttaaaggccg tctgaaatga aagagtttcc
     2701 cgatttagaa atttgagcct gattccgttc agcgggaaca ccttgaatct ttacttgaaa
     2761 tcgttgaatc cgcccaaggg ctcgcttgag tttcaatttt tcaacaagct cggtcgaaaa
     2821 cgccgcattt ttctgtttgt ccatcatgtt ttcctttatc tatccgcctc aaatagcaca
     2881 aaatcgtccg tgcccgttct cggcaatacc gcatactccg cctcgatgac gtttccgccc
     2941 aaatgtccca gctcgtccca aaccgcagcc tgttccagtg ccggattgac ttccgcgttt
     3001 gcaagcttga ttgcattttc cgcccgcttg attttgcctt ttcggcgttt ttccgccagt
     3061 tggtcgatac gcgccgtcgg gaaagcctcg cggctattgc cgttggcctg cgccttagtg
     3121 ataaacttgc cgtccatatc aaacacattg accgccgacg catcgctcaa atcgtagctg
     3181 acccgtacct cgtccttgtg atactccgcc agctctgttg agaaataaga gttgttgaat
     3241 aaatccagcc aaccgcgctg tacctttcgc acctcttgcg gcataaacat cgttgccagc
     3301 tcttccgccg acaacatatc cggcgcgata ccgtcctgtt ccagcctcat ttcccgataa
     3361 gccttcggcg tataatgccc gccgtcagga tgtcggggca gctcgccgtg cgggcggttg
     3421 ttgtattcgt ctatacattt gacaacatcc gcaatgaaac gcgaccagct cggcagtttt
     3481 ttcaaatatt tctgttgttc ctccgtcaaa tccttgcctt tttccaaagc attaaaagca
     3541 ctttccatct tgcggtacat caggttcttc gtgctgctgt ccatccccgc gcccgcaaac
     3601 gtctcatact gccgcgccat ctcaatcaga ttgtctttcc accatcgttc gatgatgcct
     3661 cgaccttgcg gattacccgc gatacccgtt tcatggcgga tacccagtcg ggacgtaata
     3721 cccgtgattt catggtctat cgtcttgccg gtttggccgc cgccgttatc cgagtagtag
     3781 ataataggca aaccaaaatg cttgaccccg atacgcagag catccgatac cgccacacaa
     3841 ctttcagcca acgaaaccga aaaaccgacc acaaaccgcg tacaaccatc aataatcacc
     3901 gtcacttccg gcttaaacgg cctgccgtgt accggatgtg ccaccttcgc tttaaagctg
     3961 tggccgtcgc cgatccaaac atcgtttggc tttaaagccc cccaatcacg tttcacataa
     4021 ggcagcagcg atttataagc cgcccccgtt ttcctgccgc gctcctgcat aatcatcggc
     4081 agcttgtccc aaacgcgccg caccatactc aaattaggca catcattgac cggcatattt
     4141 tccgcttcag cccactgcac aaaccggcgg tagctgtgcg ccagtttcgg cgcggacgga
     4201 atattgtgaa actgcataaa catcggcaac caaccgtagc tctcaatcgg cttaaccgcc
     4261 ttcgtcgtct tcggagctaa agcaaccaac cgctccgtcg cgttttccgc tttcaaataa
     4321 gcagatatcc agccgtctaa agtacgttcg ccaaccttcg ccgaccggct gcggtcattg
     4381 gccttttcca agttcccaag cgtgaccgcg tccaatttac cttctgccag caagcccaaa
     4441 aactgagcca ccgcagcctt ggcagagcaa ccgtattgat atttaatccc caacaccgcc
     4501 gccaccaccg cacatcgcgc atccgccacc gaccgttgtt tctcattcaa cagcttggcc
     4561 gcttcagcca gtgcctgagc cgacatcgcc gtccccggtc tgacttgggg caaggttttc
     4621 ggcatcttct ccgtcagctc gtccgactgc cgtttcatga tggctgctcg gatttcggca
     4681 gggagggaag cgattaagta ttttttcaga cggcctccac gcgctttgcc aacctcttcg
     4741 atgtacggcc agttctgggt ttttgctctc tcccttaatc ctcttgctgt tgtaggaagc
     4801 ccttgaatat taagtttaag cagctcacca atcgttatcc ccatcttcgt ttcctcactc
     4861 gtcaattctt aaccccagct taatcgcgat agcgcgcccc tgaccgtatc gacactttga
     4921 tttccccgtc aacacacgag taacatctac ggagttgtgt ccgttttctc tcgcccattg
     4981 cgcaactgtt aaattattgt cttttaacca ttctctaact tcatttactg ttttaattcc
     5041 cattttttta cctttcttgg tttgttcaaa tcctgtataa ttactacaaa actagctgtt
     5101 ttgctacatt tgtagcaata ttattagcta caaatgtagc tgtcaagaaa aaaggaactt
     5161 ttatggataa ttcttttgaa ttaagctcta agttattggg tgagcgacta aaaaaaatca
     5221 gaacagataa tggatactca agagagcagt tgagtgagtt atttggcatc agtagggcaa
     5281 gtattcaaaa ttatgagaat ggggaaagaa gtccgaatgc cgattactta gttcagtttt
     5341 ataagcactt tggaattaat ttgcattggc tgctaactgg aaataaggct gccaaattcc
     5401 aagatttcat tgattctgtt tcttctccga gagaagaagt actactacac ttggctcgcc
     5461 aaatggatag tcgcaccttg aatcacttga ttgactttct aatggatgtt tatgctaaaa
     5521 aataaacagc actaaggaaa actcagcttt taagttcctt ccttagtgaa cagtcgccta
     5581 aatagagaaa ttttttttaa attttgattt tgaaatgaaa aatacaaagg aaggcttcta
     5641 aacctcttcc tttgtatttt cctttgtctt gcaagaatct acaggctgag atttaaactg
     5701 gctgctgacc ggcaaaggct taccgtatct tgataaagcc aggctggaaa acgctggagc
     5761 attctctgta agcagaaatt ctgatggaat tactgacact atgggcaacc ctgtcgactt
     5821 gtcagagttt gtatttgtgc cgcgctacaa cgtagccgca gcagcagggt atggcgcacc
     5881 ggtcttcggc gaagagcctt tgttctgctt ggctttccgg aaatattgga ttgacaacta
     5941 tgtgaccaaa cagccggaaa aactgtctgt aattaccgtt aaaggcgatt caatggaggg
     6001 cgtgctcaat catggggatt ccattctcat caatcacgcc aaaaccgacc cgcgtgacgg
     6061 cctatatgtc ttgcgtatag gcaatgacct ctttgtcaaa cgtgtgcaac gcataccggg
     6121 caaactgctt gtaacgtctg aaaatccacg ttacgcaccg tttgaaatag attttagcaa
     6181 tgcccaagac gacatcgcca tcataggccg tgttgagtgg tatggccgga gtattgatta
     6241 aatccatttt aaaaacctct taaaacagtt ttaaaaattc cctaatctcg tcagctttaa
     6301 acagaaaacc gcgcattccg gcgcggtttt gtgaaaaagc tggcgtaact tttccggata
     6361 caaaaaaacg ccgaaatcca cgtctttccg agatttcagc gttttttact ctatttgttc
     6421 cttgtgcaaa aactaacagt cccccacagg aaaacgtcgc tctgttccgc cacaacggcg
     6481 aaatcctgtt gagcggcatc aacatcaaac tcaaaagcgg cgattccctg ctgatacgcg
     6541 gcccgagcgg ttgcggcaaa acttcgttgc tgcgcgcgct ggctgggctt tggccgttcg
     6601 gcagcagcgg caaagtcagc cgtccgccgc atcaagacat cctcttcctg ccgcaacgcc
     6661 cgtacacggc acagggcagc ctgcgcgacg cggtttgtta tcccgacatc gacaaacagc
     6721 atccggagct aatcgaagcc atgaacacct gccgcttggg ttatctgatt gacaaattgg
     6781 acaaaacaga cgactggcag cacaaactct ccctgggcga actgcaacgc gtcgccttcg
     6841 tccgcgccct gctttccaag cccaaaatcg tcctgctcga cgaagccacc gccgccttgg
     6901 acgaaccggc cgaagccgcg ctctaccgcg ccttaaagca aaaactgccc gacagcatca
     6961 tcatcagcat cggacaccga agcacgctca acgcgttcca caatatgcgc ctcgatgtcg
     7021 gcaacgttgc ctgcggttga acgatgccgt ctgaagccgt tttcagacgg catcgttcaa
     7081 attggaaagg cgcgcgaaaa caacgtacaa taaacccgtt taccacccct cggaatgccg
     7141 aaccgtgaac cagcttattt tcgactttgc cgcacacgac tatccgagtt tcgacaaatt
     7201 cctcggcacg gaaaacgcgg aactggtcta tgtcctccga cacaaacacg gacagtttat
     7261 ctatgtctgg ggtgaagaag gcgcgggcaa aagccatctt ttgcaggcgt gggtcgcaca
     7321 ggcgctcgaa gccggcaaaa acgccgccta tatcgatgcc gcctccatgc cgctgaccga
     7381 tgccgcgttc gaggcggaat acctcgccgt cgatcaagtc gaaaaactgg gcaacgaaga
     7441 acaagccctg cttttttcca tcttcaaccg cttccgcaac agcggcaaag gctttttgct
     7501 gctcggttcg gaatacacac cccagcagct cgtcatccgc gaagatttgc ggacacgtat
     7561 ggcgtactgc ctcgtttacg aagtcaaacc cttaaccgac caagaaaaaa tcgacgcgct
     7621 cgccagtatg gcggcggcac gccaagtaac cgtcgattcc gaaattttcg aatacctgct
     7681 caaacactgg cggcgcgata tggacagcct gatgatgatg ctcgacacgc tggacaacta
     7741 cgccgtcacg atgggcaaac gcatcaccct gccgcttttg cgccagcttt tgaaacaaca
     7801 ggaaacccaa tgaaaaacct cgccatcttc gacctcgaca acaccctcat caacaccgat
     7861 tccgaccacg cctggccgca atacctcatc aaaaaaggct tggttgacgc tgccgaaacc
     7921 gaagcgcaaa acgaaaaatt ctaccgcgac taccaaaacg gctgcctcga catcgacgca
     7981 ttcctcaaat tccacctcgc cccgctcgcc cgttacagca aagaagagct ggcggaattt
     8041 caccgcgaat ttatggcgga atacatcatc tcccacatct cgcctatgca gcgtatgctg
     8101 gtgcagagcc accaaatggc aggcgacgaa accctcgtga tttcctcaac caacgagttc
     8161 atcatcaccc ccatctgcca ccttttcggc atcaccaaca tcatcggcac acaactcgaa
     8221 accgatcccg acggtcgcta caccggcaat tacatcggta cgcccagcct caaagaaggc
     8281 aaaattaccc gcctgaacca atggcttgcc gaacgcggcg aaacgctcca aagctacggc
     8341 aaaacctatt tttacagcga ctccagaaac gacctgccgc tgctgcgcct cgtcagcgaa
     8401 cccgtcaccg tcaaccccga tgccgagctg gaaaaagaag ccaaagaaaa aggctggccg
     8461 gttttaaatt tcaaatgacg caatgccgtc tgaagcccaa ttcggcgttc agacggcatt
     8521 gaggctgaaa tccaaaaaac tcaaagccac aggaatttat ccggaaaaac aaaaaacaca
     8581 ggaatttatc cggaaaaaca aaaaacacag gaattcatcg gggaaaacaa caatctttcc
     8641 accgtcattc ccacgaaagt gggaatcttg atccgtccgc acggaaactt atatttcgtc
     8701 atttgcaccc gacataattc aatactttat tttgcaataa attaacggat gctgacaaat
     8761 tagattggat taattaccct attatctata ttgggttatc gggttgctaa ttgagcagag
     8821 cgtagcaaac cataaagtga gtgtttccac ccactctgca tttatagtgg attaacaaaa
     8881 atcaggacaa ggcgacaaag ccgcagacag tacagatagt acggcaaggc gaggcaacgc
     8941 tgtactggtt taaatttaat ccactatact atgaagtggt tattaaatat gataatgaga
     9001 cctattaaat ttagtatggt aaatatgtta ttatttattg ttatatgtag ttcatttttt
     9061 gatctgctcg ttcaattatg tacaatttta tttcatagtc aaaaaatata ctttattaca
     9121 ttatttttat tatttatttt taattttgct acaaaatcta tctatatggc aattatttat
     9181 cctattttat atttttttac gataaaaaaa tattatcctt actctaggaa agtgataatt
     9241 ctattattat tagcattatc tatatatttt agttttatgg acttttactt tttttccata
     9301 tattcagata accttagcta tgaaacggag cctttacatt tatacatccc tattattatt
     9361 aattttttct cacttttagt ttctaatttt attttatctt ttatcaacaa gtaaatgtat
     9421 atacaaaata caatccaaaa gatcctaagc aaaccaccaa tcccacacca cccttaccgg
     9481 ttcaaatccg cacaaaaacc cacatcccgt cattcccacg aaagtgggaa tctaggacgt
     9541 atag
//
LOCUS       DAWFIE010000073         9003 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWFIE010000073 DAWFIE010000000
VERSION     DAWFIE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9003
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.073"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            656..1870
                     /locus_tag="WGQ71_001655"
     CDS             656..1870
                     /locus_tag="WGQ71_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ0707629.1"
                     /translation="MSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFVDWISFTFHEDT
                     LLKVSGCPLFSDAEYMYVLSRKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE
                     VHFGGQRNTVLVELKGTGCSVASPGWELRLKQFLDDSIRTRITRIDLALDFFDGEYTP
                     DQALLDHDNGFFDNSNQRPKSETIGTAWRNEDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNYGDIEIPLDILINQGSYFCGAFPICRKFKNMPVPERFDQRKKT
                     LNLTFEHKLHYAKNAVGKLVNFMIEMGFDNSEIVESLKADSGFPKGLEPEKYALEMLR
                     DGLKHGFIHEQPDIDLEIELDELGVIAFKNSDKFDREKRLFSPDYDVEKERKYQEYLS
                     KVYHQNVDYDYF"
     gene            1882..2193
                     /locus_tag="WGQ71_001656"
     CDS             1882..2193
                     /locus_tag="WGQ71_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707630.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSRLENLKYPCEVMVMVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            2197..2397
                     /locus_tag="WGQ71_001657"
     CDS             2197..2397
                     /locus_tag="WGQ71_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707631.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEMGGGFQIFQFFVKSE"
     gene            2635..2865
                     /locus_tag="WGQ71_001658"
     CDS             2635..2865
                     /locus_tag="WGQ71_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707632.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADGFDASAIGTQVANVIMGF
                     VAMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            2893..3195
                     /locus_tag="WGQ71_001659"
     CDS             2893..3195
                     /locus_tag="WGQ71_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707633.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPECSNFEQIKQGSYVGSTVLILFVVIYGFRLLINFLKDIGKVGTD"
     gene            3542..5173
                     /locus_tag="WGQ71_001660"
     CDS             3542..5173
                     /locus_tag="WGQ71_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ0707634.1"
                     /translation="MFIILGRNFLKIILCFSFFVSKFALASVNAPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAGVGKLARLGAKLSTRAVPYVGTALLAHDVYETFKEDIQARGYQYDTETDKFV
                     KGYEYSNCLWYEDERRINRTYGCYGVDSSIMRLMSDYSRFPEVKELMESQMYRLARPF
                     WNWHKEELNKLSSLDWNNFVLNSCTFDWNGGDCSVNKGDDFRNGANFSLIRNPKYKEE
                     MDAKKLEEILSLKVDANPDKYIKATGYPGYSEKVEVAPGTKVNMGPVTDRNGNPVQVV
                     ATFGRDSQGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEVSPAENPANNPNPNENPGTS
                     PNPEPDPDLNPDANPDTDGQPGTRPDSPAVPGRTNGRDGKDGKDGKDGGLLCKFFPDI
                     LACDRLPESNPAEDLNLPSETVNVEFQKSGIFQDSAQCPAPVTFTVTVLDSSRQFAFS
                     FENACTIAERLRYMLLALAWAVAAFFCIRTVSREV"
     gene            6118..6402
                     /locus_tag="WGQ71_001661"
     CDS             6118..6402
                     /locus_tag="WGQ71_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ0707635.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     NSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            6403..7590
                     /locus_tag="WGQ71_001662"
     CDS             6403..7590
                     /locus_tag="WGQ71_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEZ0707636.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMAGDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVRKHYHIASNKMGMRTLLEW
                     KICADDPVKMASSAFSSIYTLDKKVYDLYESAEVHTVNKVKRSKWFYTLPVIVLLIPV
                     FVGLSYKMLSSYGKKQEEPAAQESAATEQQAVLPDKTEGEPVNNGNLTADMFVPTLTE
                     KPESKPIYNGVRQVKTFEYIAGCVQGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVRQSAQQHSDRAQVATLGGKPLQNLMYDNWQERGKPFEGIGGGVVG
                     SAN"
     gene            7805..8761
                     /locus_tag="WGQ71_001663"
     CDS             7805..8761
                     /locus_tag="WGQ71_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0707637.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKAYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLHQHQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
BASE COUNT         2750 a         1772 c         2157 g         2324 t
ORIGIN      
        1 gactttcaaa taatctttga atattactgt tgttctaagg tctagattcc cgcctgcgcg
       61 ggaatgacgg gatttgaggt tgcagcattt atcgggagca acagaatccg ctctgccgtc
      121 attcccacga aagtgggaat ctagtttttt gagtttcagt catttccgat aaattgcctt
      181 agcattgaat gtctagattc ccgccttata tgatgcgctc tatcaaaggg gcgcattaat
      241 tttcttaaca ttcccctttg acagccaagt gaaaggggct tttttatgtc agcagtaaat
      301 gtaatatttt cctgttctta ttggagaata tttaaaaaat cagattcttg tgttttgtgt
      361 ttttatcagt tcagacatgg cgaaccgcat aaactcatta atcaagagaa tttttcaaag
      421 ctttatcagg cgttcgatta tatagattcg gttggttcga attttccagt gattatcaca
      481 acggatggtt gtggtctttt ttgttgatct ttaaaagttt gtcaggattt ggctttcggt
      541 cgttgaccgt cgtacgcgct ttagcgcgga agacgggaaa cggctgaaag cccccccccc
      601 cttgactaac agggggggag cgaaattaaa aaccaattcc aagagtagtg aacgaatgag
      661 tgaagttgaa tatttctcac actttatatc ggacggaaaa gggaagcttt tagaaattcc
      721 gcagcgaaga ggtaagcaag acggggtttt tgttgattgg atttcattca cattccatga
      781 agatacttta ctgaaagttt ccggttgccc tttattttct gatgctgaat acatgtatgt
      841 attaagcaga aagctggaag aaattctagg ttttggcata acgcgcaaat gcaaatcaag
      901 gggcaacaaa ttctatgaat ccatgtatag gttaggttcg gatgatgttg attatggaga
      961 ggtgcatttc ggaggtcagc gcaatactgt tttagttgag ttgaaaggta ctggttgcag
     1021 cgttgcaagt ccgggttggg agttgaggct aaagcagttt ctcgatgatt cgataaggac
     1081 aagaataacg cgaattgacc tagcacttga tttttttgat ggagagtaca cgccggatca
     1141 ggcgttgtta gatcacgata atggtttttt tgataacagc aatcaaaggc cgaaatctga
     1201 aacgatcggt acggcttggc ggaatgagga cgggagcggc aagacatttt atgtaggtcg
     1261 caagaaaaat tctcgttttg ttcgtgttta tgagaaaggc aggcagcttg gagataaaga
     1321 aagcaaatgg gtaaggttcg agatccagtt taattatgga gatatagaaa tacccttgga
     1381 tattttaata aatcagggtt cgtatttctg tggagctttt ccaatttgta gaaaatttaa
     1441 aaatatgccg gttcccgaaa ggtttgatca gagaaagaaa acgcttaatt taactttcga
     1501 gcataaattg cattacgcga aaaacgcggt tggaaaactg gtcaatttca tgattgaaat
     1561 gggttttgat aatagcgaaa ttgtggaatc tttaaaggca gattcgggat ttcccaaagg
     1621 attagaacct gaaaaatatg ctctggaaat gttaagggac ggtttgaaac acggttttat
     1681 tcatgaacag ccggatattg atttggaaat tgaacttgat gaattggggg ttattgcttt
     1741 taaaaattct gacaaattcg atagggaaaa aaggcttttt agtcctgatt atgatgtcga
     1801 gaaagaaagg aaatatcagg aatatttaag taaagtttat catcaaaatg tagattatga
     1861 ttatttttaa aggaaatcaa aatgtttaat caaactcaaa ctgtaactta tcctgcaact
     1921 tttttgggag ccaaaaaatt caaaggtgaa attgatggct ctaacattga cacttgttcc
     1981 gtattggttg caacaccttt accggcacag tcgggtaatg ctgttggatt tacggcagca
     2041 caaatgaagt tcggggatag taagaatttc tcaaggttag agaatctcaa atatccgtgc
     2101 gaagttatgg taatggttga aatgacttcg acaggtaaag gcatggttcc gtcattaatt
     2161 gattttcagg tggcggaaaa gccgaaaggt tgatttatga aatttgaaga acgtttcata
     2221 gttcaagatt tagaaacgca tgactttatt tatcccgatc cgttcggtga tgtggggttt
     2281 actcaaaata ttaaatcagc aggtcaattt gaaagttacg aagatgcgtt gaattcaggc
     2341 ataaatgaaa tgggcggagg attccagata tttcagttct tcgtaaaatc ggaataaaag
     2401 aaaaacaggc tcggcgggcg gtctgttaac ctttcacaaa gcccgcaaca aaggaaaaat
     2461 atcatgaaaa tgaaccttgc aacactaatt atcggctggg tggtctgtat gtttcttttt
     2521 cttttcgcaa tcctctattt tatcggctaa aaacgagatt cggaaaagac ttcgtccgga
     2581 tgaagcaagt caagaagtcg tcttatttta aatatcaaaa aaaggaaaaa aacgatgaac
     2641 atcgttaaaa aatatgctgt aaaagcattg ttggcagctg gtatcttcac accggctatt
     2701 gttatggcag atggctttga tgcatccgcg attggtacgc aagtggcgaa tgtaatcatg
     2761 ggttttgtgg caatggtttc cgccgtgggt atggcggcca ttacggtcat cttggcaatc
     2821 caaggcttca agatggcttg gagcatgatc aaatctgtca aataaacaga gtgaagaaaa
     2881 aggggcgcat aaatgggcta tcgtgtcggc ataaattgtt ttgatacaag attgcaggca
     2941 gacgactatt tattgtcgtc ccttcctcct actgttaccc aggacggaaa aatcatcagg
     3001 ccggaaaggg tgggcgataa atggattttg aacggaaagc cggttacgtt gtcttatccg
     3061 gaatgttcca attttgagca gataaagcaa ggttcttatg tcggttcgac ggttctaatt
     3121 ctgtttgtag tcatttacgg tttcaggctt ctgattaatt ttttaaaaga cataggcaag
     3181 gttgggactg attgatgatt atagatttct ggtttcttct cggtttcttc ttggctttgt
     3241 ctgttgcttg gctgttttgg taacggttgg tagaatcggc tttttagagt gttttaaaag
     3301 gtccgaatta tgtttatttc tgaatatcat ttagttaaat ttcaaactga ttcacatatt
     3361 tatagagatt taccacaagc gttaatttat tatagggaat tgattagaaa aggggttttt
     3421 aaaacttcgt tttcatttga tatttttagg aatttctttc atcgttatga tagagatttt
     3481 atagaaattc aattccctga ttcttctaca ttattaatta aattagatga agcaaaatgt
     3541 tatgtttatt atcctagggc gaaatttttt aaagattatc ctatgcttta gtttttttgt
     3601 atctaaattt gcattggcat cagtaaatgc tccgggtaaa tttgataggg ttgaagttta
     3661 tgatgatggc agatatttag gtattcgagg ttcagatgac aaaagaagaa gaatttggaa
     3721 aggtgtattt gatagagaat cgggaagata tttaacttca gaagctcaag atttaaaagt
     3781 taggcatgta tctactggag catcaagtac gggtaaagtt agttcggttg tatcttcatc
     3841 agtttcccgc gctggcgtat tggcgggggt cggcaaactt gcccgcttag gcgcgaaatt
     3901 aagcacaagg gcggttccct atgtcggaac agccctttta gcccatgacg tatacgaaac
     3961 tttcaaagaa gacatacagg cacgaggcta ccaatacgac accgaaaccg acaaatttgt
     4021 aaaaggctac gaatatagta attgcctttg gtacgaagac gaaagacgta ttaatagaac
     4081 ctatggctgc tacggcgttg acagttcgat tatgcgcctt atgtccgatt acagcagatt
     4141 ccccgaagtt aaagaattga tggaaagcca aatgtatagg ctggcacgtc cgttttggaa
     4201 ttggcataaa gaagaactga ataaattaag ttctttggat tggaataatt ttgttttaaa
     4261 tagttgcaca tttgattgga acggcggaga ttgttcggtc aataaaggtg atgatttcag
     4321 aaatggggct aatttttccc ttatccgcaa tccgaaatac aaagaagaaa tggatgccaa
     4381 aaagctggaa gagattttat cgttgaaagt cgatgccaat cccgacaaat acataaaggc
     4441 aaccggttat cccggttatt ccgaaaaagt agaagtcgca cccggaacaa aagtgaatat
     4501 gggtcccgtc acggacagga acgggaatcc cgttcaggtt gtcgcaacat tcggcaggga
     4561 ttcgcaaggc aacaccacgg tggatgttca agtaatcccg cgtcccgact tgacccccgg
     4621 aagcgcggaa gcaccgaacg cacagccgct gcccgaagta tcgcccgccg aaaaccccgc
     4681 aaacaacccg aaccccaatg agaaccccgg cacgagcccc aatcccgaac ccgaccccga
     4741 tttgaatccc gatgcaaatc ccgatacgga cggacagccc ggcacaagac ccgattcccc
     4801 cgccgttccg ggacgcacaa acggcaggga cggcaaagac ggaaaggacg gcaaagatgg
     4861 cggccttttg tgcaaattct tccccgacat tctcgcttgc gacaggctgc ccgagtccaa
     4921 tccggcagaa gatttaaatc tgccgtctga aaccgtcaat gtagagtttc agaaatcagg
     4981 aatctttcaa gattccgcac agtgtcccgc acctgtcact ttcacagtga ctgtgcttga
     5041 ttcaagcagg cagttcgcgt tcagctttga gaacgcatgt accatagccg aacggctaag
     5101 gtacatgctt ctcgcccttg cttgggcggt tgccgccttt ttttgtatcc gcacagtatc
     5161 tcgtgaagtc tagcaggcgc agcaccgccg ggcttcagta acttgtacca aggcaggggg
     5221 aggacgtcca gaaagatttg taaagacggc tttatcgtct ttataaatct ttttggatac
     5281 cccttgccgc cccgccaaaa gaacacattc tgccgcaagg gcaggtggta aggcgcgcgc
     5341 cttttgcgcc gttccccctg cccccgcggc gtcgcaagtg agactggggg tgcgggggct
     5401 agtccccgca aagcctttca gcttcggaag ccacggccga aaggcaggcg cagcactgcc
     5461 ggtctgagcg gaagccaggc tacaggcagg cgcagcaccg ccgagctagg cggaagccag
     5521 gctacaggca ggcgaagcac cgccggttgg gcggaagcca cggccgaaag gcagggcgaa
     5581 gcaccgctag gcttaggcgg aagccacggc cgaaaggcag gcgaagtacc gccggtctgg
     5641 gcggaagcca tggtaaaagg caggcgaagc accgccgggc ttcagtaacc tttgttcagg
     5701 cagggggagg atgtccgtaa agaatcgtaa aggcgggttt ttttcgcctt tatgattctt
     5761 tatggatacc ccttgccgct cggacaaaga aatacactct gccgcaaggg caggtggtaa
     5821 ggcgcgcgcc ttttgcgccg tccccctgcc cccgcggcgt cgcaagtgag actagggggt
     5881 gtgggggact agtcccccgc aaaacgttca gcttcggaaa ctttggccga aaggcaggcg
     5941 aagcagcgca ctttgcgacg aatgtcgcaa atagccgaga agcgcggggg gattggcgat
     6001 aagcgcgagg ggggtgtccc cacagcgccg ccgcgccgcg aatgcggcgc aaaatctttc
     6061 agattaagaa acatttgttt aatgaggcaa ccgtgccttt taagaaaggg atagcaaatg
     6121 aaattgttgg ccgcattgat tccgcttttg atgagcgtgg caggccgtat attgactgca
     6181 ttaggcttga tggcggtaac ctattcaggg gtggatagat tggtagccca ttttcagcag
     6241 gcgataacca atagcataac gggcgcgcct caagcgatgt tgcagctttt ttatataagc
     6301 ggcggtggaa ccgttcttaa tatcctgttt ggtgcgatcg cctttattct gtcatttaaa
     6361 caaatgacaa aactagcaac ctcaatcggg aagaaaaaat aaatggcaga gatctgtttg
     6421 ataacaggca cgcctggttc agggaaaaca ttaaaaatgg tttccatgat ggcaggcgat
     6481 gaaatgttta agcctgatga aaacggcata cgccgtaaag tatttacgaa catcaaaggc
     6541 ttgaagatac cgcacaccta catagaaacg gacgcgaaaa agttgccgaa atcgacagat
     6601 gagcagcttt cggcgcatga tatgtacgaa tggataaaga agcccgaaaa tatcgggtct
     6661 attgtcattg tagatgaagc tcaagacgta tggccggcac gctcggcagg ttcaaaaatc
     6721 cctgaaaatg tccaatggct gaatacgcac agacatcagg gtattgatat atttgttttg
     6781 actcaaggcc ctaagcttct agatcaaaat cttagaacgc ttgtacggaa acattaccac
     6841 atcgcttcaa acaagatggg tatgcgtacg cttttagaat ggaaaatatg cgcggacgat
     6901 cccgtaaaaa tggcatcaag cgcattctcc agtatctata cactggataa aaaagtttat
     6961 gacttgtacg aatcagcgga agttcatacc gtaaataagg tcaagcggtc aaagtggttt
     7021 tacactctgc cagtaatagt attgctgatt cccgtgtttg tcggcctgtc ctataaaatg
     7081 ttgagcagtt acggaaaaaa acaggaagaa cccgcagcac aagaatcggc ggcaacagaa
     7141 cagcaggcag tacttccgga taaaacagaa ggcgagccgg taaataacgg caaccttacc
     7201 gcagatatgt ttgtcccgac attgacggaa aaacccgaaa gcaagccgat ttataacggt
     7261 gtaaggcagg taaaaacctt tgaatatata gcaggttgtg tacaaggcgg aagaaccgga
     7321 tgcacctgct attcgcatca agggacagca ttgaaagaag tgacggagtt gatgtgcaag
     7381 gactatgtaa aaaacggctt gccgtttaac ccatacaaag aagaaagcca agggcaggaa
     7441 gttcggcaaa gcgcacagca acattcggac agggcgcaag ttgccacgtt gggcggaaag
     7501 ccgctgcaaa atcttatgta tgataattgg caggagcgag gaaaaccgtt tgaaggaatc
     7561 ggcgggggcg tggtcggatc ggcaaactga agaaaacggc aagagagaaa aaagacccat
     7621 aaaccgtttg aatatagacg gcttacgggt ctttgtttcg cgcaaagcaa aggctaaggc
     7681 agtcaggcag caaatcccgc aatgtatcaa aacagacgcg tagaaatgcc ggctgccttt
     7741 atccatcctc aaaattgaat atcatcctag ccgtatcaag gctgtataaa taaggaaaat
     7801 accaatgaat ataatcgggc tggacatctc aaaggacacc atagacgcaa cattgcataa
     7861 aacaaacgga agtatccatt acattaaatt taagaataat gatgatggat taaaacagtt
     7921 tagattgtgg ataaagggaa acagaatcag aaaagcctat atcggcatgg aggcaacagg
     7981 catctattac gaaaaggcag cagatatgct ttcttcctac tatactgttt acgttattaa
     8041 tcccttaaaa atcaaggact acggaaaaag caggtttaac cgtaccaaaa ccgacaaagc
     8101 agattcaaac ctgatagcag actacataaa aaggcatcaa gatacattga taccgtatca
     8161 gatacccaaa aacaaagcac tgcaaaaact gattaacctt aaaaatcaat tacatcaaca
     8221 tcagaagcaa attaaaaacc gtcttcatag cactgaagaa gacttcataa ggaacataca
     8281 tcaagacttg atagatacca tacaggacaa gatggaacag gtaaaaatag ccatatccga
     8341 acaaatcaaa aaacaaacgg acaataacca ttaccgcaat cttcaaacca tcccgagcat
     8401 aggcaaagac accgcatcag ttctttatgc gcaactgaca gaaaaacatt ttaaaaccgc
     8461 aaaccagttt gtatcctatg ccggattaag tcccgccatc atacaatcag ggacaagcgt
     8521 aagaggtcgg ggcagattga gccgatacgg aaacagacga ttaaaaagta cgctgtatat
     8581 gcccgccctt tgtgcttacc gttttaacgc atttccgaaa ttaataaata atctgaaaaa
     8641 agcgggtaag ccaaagatgg taatcatcgt tgccatcatg cgcaaactgg cgaagctcgc
     8701 ctattacatt gttaaaaccg gccagcctta cgatgcggaa agacaccgat tgaatcaata
     8761 aaattcaaca aaattaaacg gttacgcgaa tatatttgtg taaccgtgca tttgcatatc
     8821 gtaaataaac gtaaataaaa ataacaatat aaatcagtat attgcaactt tgttttttat
     8881 tttgtgttga cgggcaacat atcatctgcg cgggaatgac gaatccatcc atacggaaac
     8941 ctgcaccacg tcattcccac gaaagtggga atctaggacg caaaatctca agaaaccgtt
     9001 tta
//
LOCUS       DAWFIE010000074         8906 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWFIE010000074 DAWFIE010000000
VERSION     DAWFIE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.074"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(127..339)
                     /locus_tag="WGQ71_001664"
     CDS             complement(127..339)
                     /locus_tag="WGQ71_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0707638.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWTEKTNAV"
     gene            complement(351..632)
                     /locus_tag="WGQ71_001665"
     CDS             complement(351..632)
                     /locus_tag="WGQ71_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707639.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(679..942)
                     /locus_tag="WGQ71_001666"
     CDS             complement(679..942)
                     /locus_tag="WGQ71_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707640.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(962..1536)
                     /locus_tag="WGQ71_001667"
                     /pseudo
     CDS             complement(962..1536)
                     /locus_tag="WGQ71_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1511..1855)
                     /locus_tag="WGQ71_001668"
                     /pseudo
     CDS             complement(1511..1855)
                     /locus_tag="WGQ71_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1848..2723)
                     /locus_tag="WGQ71_001669"
     CDS             complement(1848..2723)
                     /locus_tag="WGQ71_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ0707641.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELTEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHEISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2768..3058)
                     /locus_tag="WGQ71_001670"
     CDS             complement(2768..3058)
                     /locus_tag="WGQ71_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0707642.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3070..3246)
                     /locus_tag="WGQ71_001671"
     CDS             complement(3070..3246)
                     /locus_tag="WGQ71_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0707643.1"
                     /translation="MAITVEKLKENFAKNGQTLAQWARENGFKPRDVYLVVGGQRKGN
                     YGKGHEIAKKLGLK"
     gene            3364..3735
                     /locus_tag="WGQ71_001672"
     CDS             3364..3735
                     /locus_tag="WGQ71_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEZ0707644.1"
                     /translation="MLFVGFLERIKQELDLKKDKDVAEFLGLEPKAFSIRKKRDSIPV
                     DAVRAAAFEHPELNADWGYILTGRREKSVKMPSEKLNKEEQELLALFREAAAADREMI
                     LMVARRAEKKAQAALGKVSNG"
     gene            3740..4042
                     /locus_tag="WGQ71_001673"
     CDS             3740..4042
                     /locus_tag="WGQ71_001673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707645.1"
                     /translation="MNTVLTPKLIEKLERLRSLEIEAQELRAELQPWLDNACKQYFVA
                     WLENQLEKGRDISVLELKSNLSDLAYHAVMNDNIALWHQVSEVKKLLAIYEAKRKT"
     gene            4261..4467
                     /locus_tag="WGQ71_001674"
     CDS             4261..4467
                     /locus_tag="WGQ71_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HEZ0707646.1"
                     /translation="MMFASPTPALAKSCKDFPTQQAAQKFYEARKKAGLSGWKSLDRD
                     GDGWACDCNPGGSGKNCPKKKDNF"
     gene            4478..5065
                     /locus_tag="WGQ71_001675"
     CDS             4478..5065
                     /locus_tag="WGQ71_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707647.1"
                     /translation="MKTKIYLGIFLLAAAHLVNAGGVMKTGGMIGPHPETFSEQFNEL
                     NNGMMDLFPRISIVAGKTRQTKDLGNGITLSAKLVKEGDIFENVRINCNTLAKTQRIN
                     NCLVSMYYTAIALDYEIDRLAFMNAIQTAIKRMHATYNQNGVDYFITVNRKQKSVSMF
                     AKSDMPMQIEAMDDSNEAYMKSDKCNPEIELCIKP"
     gene            5160..5663
                     /locus_tag="WGQ71_001676"
     CDS             5160..5663
                     /locus_tag="WGQ71_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ0707648.1"
                     /translation="MSDEFNRFIERVLSHEGGYANHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAVRMLQRAAG
                     VADDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLSTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            5775..6107
                     /locus_tag="WGQ71_001677"
                     /pseudo
     CDS             5775..6107
                     /locus_tag="WGQ71_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6104..6514
                     /locus_tag="WGQ71_001678"
     CDS             6104..6514
                     /locus_tag="WGQ71_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ0707649.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLTAEMAALKKPDALIHGKEVRQKERNSSQG"
     gene            6519..7268
                     /locus_tag="WGQ71_001679"
     CDS             6519..7268
                     /locus_tag="WGQ71_001679"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ0707650.1"
                     /translation="MPSVELLLEIVGLPRGTFYYQLAVQSAEDKYADLKQHIHDIYRR
                     HKGRYGYRRIAAAILHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNI
                     LRRCFHAEKPNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEI
                     LKGAPEKPGSSEKPILHSDQGWQYQMFFYSGLNLNQDKAMKPQTVQIVRQGEATPYWF
                     KFNPLYNNDRIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(7587..7949)
                     /gene="ssrA"
                     /locus_tag="WGQ71_001680"
     tmRNA           complement(7587..7949)
                     /gene="ssrA"
                     /locus_tag="WGQ71_001680"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8012..8617)
                     /locus_tag="WGQ71_001681"
     CDS             complement(8012..8617)
                     /locus_tag="WGQ71_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ0707651.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT         2104 a         2074 c         2360 g         2368 t
ORIGIN      
        1 gagtgagttt attttggggc ggcggcaggt cgggggcaag cggcgtgggg cttggttgtg
       61 gtttttaggt ttgggggtgg gtaaaaaatg ccgtacccgg cgggcaggct tcgcactctg
      121 aaggtttcag acggcgtttg ttttttctgt ccaatcgagg aactgccgcc atttttccag
      181 cggcatatcg gcccgccgcg tttcggtatc gggttcggct tcccagcggc ctacctgtat
      241 gtagtgcttc accccgacga tttgcgccaa ctcggcctgt gtcagtttgc agcggttgcg
      301 cagggtgcga aggttgtaag gcgtgtagcc gagttccatg tcgttgcggt ttattttgtt
      361 tcgcatattt ttttgactgc ccggcggcag gtttcggtaa ggacggcggc aaatcgggct
      421 tcgtctgccg gttttccgtc gaaaaaaata aaatcgtaaa gggtgatgcc gttttcggaa
      481 tggcggtaat gcccgcccgg gcattcgctg tctgtctgcc tgtggatttt ggcaatcagg
      541 cggggatagc ggcaatgggt aatgaaacgg cttgcgccgt cttgcccgat aatccattcg
      601 gggtagcggt tgaatagtgc ggctctgttc attttgttcg ggttgttcgg gggataaagc
      661 ccctcgcggg gcttgtggtc aggcaaattt gaatatcagt gccaacacgg cggcgatggc
      721 ggtaaccagc ccggtggccg ccatcatggg ataccatcgg gattcttggg ctatttttac
      781 ggattcggcg ttgattttgt gcgcgtctgc aatgattttg gcgatttcgg catctacttt
      841 tctcagactg gagtgtttca tttcttgttc gattatgttc atcgtacttc ctttcgtttt
      901 tggcggttgc cgccgcttgt cggatggtag gatgtctgcc atgtgtatat attgatacct
      961 tttaggtttt attgcaagtg ttttgggcgg cggcttcgta tgcttggcgg tggcggcggc
     1021 tgtaccattc ggcgagttcg cgccgctctt ggaggcggta gctgtcggcg gtcaggtagt
     1081 ggtgcaggct tgagaagccg gcgcgttgga gggcggcgcg gctgatgtgg ccgagggcga
     1141 ggtctgttgt gagggtgtct tttccggcgg tcaggctgat gttggtgaag aggtggcgga
     1201 atccgtgcat tgtgtgtttg gatttgccgg gggtgctgcc gtcatagccc agtcgtcgga
     1261 tggcgttgtg ggcgaatttg atgctgatgt ggtcgggatg gggggggcgg gtttgcgccg
     1321 tgggcggatg ccggggaaaa ggtggatgtt gtcgccggtc tgcgtgtgca gctctcggag
     1381 tatttctacc gcccagtccg acagtgggac ggtaaagggg tgtttggtct tcatgtcggc
     1441 ggcggggatg tgccataacc gggcggtgag gtcgatgtct tgccagcggg cggaaagcag
     1501 tgcggacgga cgggatgact tccccggttt ccccatcaat ccgcgccgcc tcgatttttc
     1561 cgttggtgcg cagcatttcc accgcctgcc gcaccgacat caaccgcttg ccgttgcgcc
     1621 tcatcgcctc caccaaaacc gccgaaatca atttggcttc ttccgtttta agctccgtct
     1681 tgcccgcatc gctgcgccgt ttgcgcgtcg gcttgatgct gaccgcctcc agcttgcggt
     1741 atagcgtggc aaggctgatg cccaattcct gcgcctgctg cttaagatat gcagagcgtg
     1801 cgccgcgtcc cattgcttcc gcctgattct cgactgcctt aagacgctca atcattgccg
     1861 gattcatcgc cttctcccgt ttcaccgccc aaccattccg gcacattgtc tgtcggtgct
     1921 tcagtcggca gggcatagct ttcgcgcagt tgctcgcagt ccaaaataat ttgattgagc
     1981 gtgccgacca tctttgcctg atggctgatc tcgtgtgcct cactgtgcgc attaagttgg
     2041 tcgaacaaat ctttcaaacg gctcacttga ctgcggatac cgacctcaag gcttgttaac
     2101 tgcatcgcca actcactgcc cacgtcttcc gccttcggct ctctgacaac ggtttgcttc
     2161 ttagccagct tctcggtcag ctcatcaatt ttggctgttt tggttttcat cacttcgtct
     2221 ttggcggcga ggttttcacg gctttcgcgc agggcgacgc gcagctcgcg caccgtcatt
     2281 cggtccacat cgtcaaaggt catgccgttg acttcttctc cttcggcaag tcccaccaac
     2341 gtaacgtctt cttcgaccaa gagttccagc agctttgact tgcccaaatc catcagcttc
     2401 ggcgcggctt tctgcatttg cggggtcgca aagcggcgag tggctgacat cagacgtgat
     2461 gtttctgcga tacccagccc aaattggctt ttcacaattt ccataaaccg tccgtgttcc
     2521 gtatgctctt ttaaaataat cagcgcacgt cccagttcga acatgccttc catcgtctgc
     2581 cgtactgctt gacgaccgcg ttcaacccaa cgctcttcgc tataagtctc gccgttaccc
     2641 cactgctcca tcaccagcac actgcgcatc gctgcctgat tgcttacttt gtcggttgcg
     2701 ataatttcaa tttctgtatt cattttttat ctccaaagtt tccgacgtcg gaaactttca
     2761 aaatccgtta atccacatcg acccgcttgc cgatttcggc aatcttgctt tgcagccgtt
     2821 catgctgctg cctgaaccgc tctgcgattt gcagggtttt gatgccgtag gcgtagttgc
     2881 cgttttcaag tttgatgacc aatcccgagg caaccaaatc atcaatatcc ctgctgactt
     2941 gcgatggcgt cagccccagt ccgaccgata aatccttatt gctcagaccg ataatcggat
     3001 gctcgtcaag cgcgataaag accctcaata gccgttgtac ccttttactt tctgccatcc
     3061 gcatcctcct tatttcagtc ccagcttctt ggcaatttca tgccccttgc cgtaattgcc
     3121 tttgcgctgt ccgccgacca ccagatacac atcgcgcggc ttaaagccgt tctctcttgc
     3181 ccactgcgcc agcgtctggc cgtttttggc aaaattttct tttagttttt caacagttat
     3241 agccatagat agccctttcc cattgtgata aaatagtgaa ctatttatgc accagatatt
     3301 tactagtgcg ctccataaac agaatattaa tctacttttg tagattattt caagagattt
     3361 tgtatgcttt ttgtgggttt tttagaaaga attaagcaag agcttgattt aaaaaaggat
     3421 aaggatgtcg ctgaattttt aggactagag ccgaaagcgt tctcaattcg aaaaaaacgc
     3481 gactcgatac cagtagatgc agtacgcgca gcagcatttg aacatccgga attaaatgct
     3541 gattggggat atatcttgac tggacggcga gaaaaatcag taaaaatgcc atctgaaaaa
     3601 ttaaacaaag aagagcagga gctgctggcc ttgttccgcg aagcggcagc tgccgaccgt
     3661 gaaatgattc tgatggttgc gcgcagggca gagaaaaaag cccaagctgc gcttggtaaa
     3721 gtgagcaatg gataatgata tgaatacggt gttgacgccc aaattaatag aaaaattaga
     3781 acgattaaga agccttgaaa ttgaggcaca agagttgaga gcggagctgc aaccttggtt
     3841 agataatgct tgcaaacaat attttgttgc atggcttgag aatcaactgg aaaaaggtcg
     3901 ggatatttct gttttggagc taaaaagcaa tttgtcagat ttagcttacc atgcggtgat
     3961 gaatgacaat atcgcattgt ggcatcaggt aagtgaagtg aaaaaattgc ttgctattta
     4021 tgaagctaag cggaaaacat aaaaaatgcc gtctgaaatt ttcagacggc atttaggaca
     4081 ctccgacagt tgggctcggt gttctgaaac ggatattaag caattgaagc ggccgtgcat
     4141 tttaaggcgc attaaaaacc accccgatat tcaggtcatc gggcaggtgg ttcaggtctc
     4201 gaaaaacctg tattaaagag acaaaatgaa aagggttatt tgacattgct tgtgtctgtt
     4261 atgatgtttg cttcacccac gcccgccttg gcgaaaagct gcaaagactt tccgacgcag
     4321 caggcggcgc agaaatttta tgaagcccgc aaaaaagcag gattaagcgg ctggaagagt
     4381 ttggacaggg acggcgacgg atgggcttgt gactgcaacc cgggcggaag cggcaagaat
     4441 tgtccgaaaa aaaaagataa tttctgaaag aaatagaatg aaaacaaaaa tatatcttgg
     4501 aattttctta ttggctgctg cacatctggt caatgcggga ggagtgatga aaacaggagg
     4561 gatgattggt cctcatcctg aaacattttc ggaacagttc aatgaattga acaatggaat
     4621 gatggatttg ttcccaagaa tatcaattgt tgcgggcaag acacgtcaaa caaaagacct
     4681 tggtaatggc attacgttgt ctgcaaaact ggtcaaggaa ggagatattt ttgagaacgt
     4741 ccggataaat tgtaatacat tggcgaaaac tcaaagaatc aacaattgct tagtgagtat
     4801 gtattacacg gctattgcgt tggattatga gatagaccgt ttggcattta tgaatgctat
     4861 tcagactgct atcaaaagga tgcatgctac ttataatcaa aatggggtag attatttcat
     4921 tacggtcaat cgtaagcaga aaagtgtttc tatgttcgca aaatcggata tgcctatgca
     4981 gattgaggca atggacgatt caaatgaagc atatatgaaa agcgataaat gcaatcccga
     5041 aatcgagctg tgtattaaac cttagcctca ggaaggtttt aacctccatt aaaagcccgt
     5101 tcagacggcc tttcctacaa tccccgtatt gatttcaacc tcaatacggg gatttttcca
     5161 tgtcagacga attcaaccga tttatcgaac gcgtcctctc ccacgagggc ggttacgcca
     5221 accatcccaa agaccctggc ggcgaaacca attggggcat cactaagcgc accgcacagg
     5281 caaacggcta caacggctcc atgcgtgcca tgacgcgtga acaggctatc ggcatttacc
     5341 gaaaggcgtt ttgggagcgt taccgcgccg accaaatgcc ggaagcggtc gcgttccaat
     5401 tttttgatgc ctgcgtcaac cacggttacg gcaatgccgt ccgtatgctg caacgcgccg
     5461 caggcgtggc ggacgatgga atcatcggcg aaatcagcct caaagccatc aattccctcc
     5521 ccgaaaacga ccttttattg cggttcaacg ccgagcgtct ggtcttttat accaagctca
     5581 gtacgttcac ctctttcggc aagggctggg tacgccgtgt ggcgcaaaac ctgattcacg
     5641 cgtctgcaga taacactgat taaagggaga taaaccatgt caaaaaagtc actcatcgcc
     5701 ctaatgaccg cagccatgca gcccgatttc agccacagcg acctaggcat tcgctacgcc
     5761 atgccgactc agggatgttg gacgcaagcc caccgcaaga gcggggtagc cgccgcgaaa
     5821 cgcgcagcca aaaaaaagcg tcacaaataa ccgccttttt ccgatggctg ggcggcttgg
     5881 tctctaatcc ggccacagga aaaatcagcc ataccaaact atgggcaaac gtcgccgcag
     5941 ccgccatgac ttggaagttc gtgcaggcgg cggacgcgcc cgaatggctc tagtgggctt
     6001 atggcgcatt ggtcggcggg tatgcattaa tcaaacgcgg catcgcggcg attccgcagt
     6061 tggcagaaat caaaaaatcc gcaaatcagg aaggggggcg gcaatgattg aatttgtccg
     6121 agccaaaaaa cggctgcttt gggcatttgt gcttttgctt gtgtggacgt gcggttaccg
     6181 atacgccgcc gacaaggccg aagcggaaca aaccgccctg attgccacct atcggcattc
     6241 ttctatggtt gcggcggaac aatatgcctt gcagcttaaa aaagcgcagg acgaaaggca
     6301 gcggtggtac gacttttccc aaaaacaagg aagaaagccc gtgaaaaaac agtatccgcc
     6361 gcaaacgaaa aaagccggct atctgaaaac caaggaagaa ctgcttgcgg aattggcttg
     6421 ccttacagcg gaaatggctg ccctaaaaaa gcccgatgcc ttaatccatg ggaaagaagt
     6481 gcggcagaaa gaacgcaact cgtcgcaggg ttaaggcaat gccatccgtt gaactgctgt
     6541 tggagattgt cggactgcca cgcggcacct tctattacca attggccgtc caatcggcag
     6601 aagacaaata tgccgatttg aaacagcata tccatgatat ttatcgacga cataagggaa
     6661 gatacggcta ccggaggatt gcagcagcca tccttcatgc aggaacaccg gtcaatcaca
     6721 agaaagtcag ccgtctgatg gcgaagacgg ggctgaaggc agtgatacgg cggcgcaaat
     6781 accgctcgtt caaaggagaa gtcggcaaaa ttgcgccgaa tatcctgcga cgctgtttcc
     6841 atgcagaaaa gccgaatgag aaatgggtaa cggacgttac cgagttcaat gtaggcggag
     6901 aaaagatata cctttctccg attatggatt tgtttaacgg ggaaatcgtc agttaccgta
     6961 ttcaaatccg cccgactttc gatttggccg gcgagatact gaaaggtgcg ccggagaaac
     7021 cgggatcgtc tgaaaagccg atactgcatt cggatcaagg ttggcaatat cagatgtttt
     7081 tttatagtgg attaaattta aatcaggaca aggcgatgaa gccgcagaca gtacaaatag
     7141 tacggcaagg cgaggcaacg ccgtactggt ttaaatttaa tccactatac aacaacgata
     7201 gaatcaagtt gaaattaaaa gggctgagcc ttgttcagta cagaattcag tccctgaaag
     7261 ccgcttgatt aaactgtccg actttttggg gtcagttcgg cttcggcatt tttttatccg
     7321 tttgggggta acttgtttgg aaagctgcaa gcttataaat aaaggattac atttaagttt
     7381 tgggtaacct ttttaaaaaa atgcgtgatg acttttgcat ttttaaggcg ttttttgggg
     7441 taattcgtga aaagttaccc caaaagttac cccataaatg gcgaaaactc aagcatacgc
     7501 cagcatattc aaggcaacaa aaaagccttg aaactgttga agttcaaggc ttttttgtgt
     7561 tgcaggatgc tgctgaaaat agggtatggt ggaggcgggg ggaatcgaac ccccgtccga
     7621 aagtcctcta caaagcgttc tacatactta gttgtgtcta tttgaaaatc ttatttccat
     7681 catgccgacc aacaggcctt ttggaaacca gttaccttaa atctcatttc ctgccaagta
     7741 acccggtagg aaaccagtca atgtaagatg acgttgcggt ggctttcgcc acacagccca
     7801 ttgaccgact gctgcaacgg ctagccttaa gcggctaaag cgtaagtttc gtcgtttgcg
     7861 actatttgaa ttcagtgttt tacgggaatc tgagaccccg gtatgcccgc atctgcttcg
     7921 caacccccgt cgaaaccaag gtcgccccca gaaatggttt gcaaattata cggatattgt
     7981 gcggtgctgc caagtctgtc ggagaaattt gtcagtcttg ctgccttaat ttgcgtttga
     8041 gcaggatgcg gacgcagccg tcgttgcctt cccggggttc ggcgtaggcg agtacgtcgg
     8101 ggtgttgcat cagccagttt cgggtcatat ttttcagaac gggtttgtag cctttggaac
     8161 ctaatccgct gccgtggatg atttcgccgc atacgccgcg tttttgggtg aatgcgatga
     8221 attcgttgag gactttttgg gcttcttcct gtgtgtagcc gtgcaggtcg acatcggtaa
     8281 cgacgggata gtatccgttt ttcaggcgtt ggatgtcgtt ttttccctgt ccgtttttgc
     8341 tgaagctggc gggcgggtcg ttgtatgtgc tgccgatgta gaagtagttt tcttcgtcgg
     8401 cgcggttgtc tttgggacgg actttgatgg gggttttgtc gggcggcgca taatattgct
     8461 gccggttttt taatggggag acttgtccga ctgcttgtga aaaatcgaaa tcctgttctt
     8521 gtttttgctt gtttttttcg gctgcctgtt tttctgccgc ttctttttgg gcttgtttgc
     8581 ccagttgttt gaggatgttt tggaagtcgg tattcatatt ttttcctgtt atttgtccga
     8641 tggctgtttc gggcggggtt ttaatttgcc ggaatgtttg ccaatcgggg gaggatgatt
     8701 ttgttgcctg cgtatgtttt ttgaaagtgt gattgtatat caaaaagaaa tgcggcaacc
     8761 gtcggcagtg ttgattgccg gaaatgcgga ccggtcgaac cgatatgccc gaacgcctga
     8821 taaagtttta aaaacctgcc ttgcgaagca ggctgacgtg ttttgccaat cttgaattgc
     8881 cggaaacgcg aaacacggaa atctga
//
LOCUS       DAWFIE010000075         8868 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWFIE010000075 DAWFIE010000000
VERSION     DAWFIE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.075"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(392..826)
                     /locus_tag="WGQ71_001682"
     CDS             complement(392..826)
                     /locus_tag="WGQ71_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ0707652.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(829..1491)
                     /gene="exbB"
                     /locus_tag="WGQ71_001683"
     CDS             complement(829..1491)
                     /gene="exbB"
                     /locus_tag="WGQ71_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ0707653.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1557..2399)
                     /locus_tag="WGQ71_001684"
     CDS             complement(1557..2399)
                     /locus_tag="WGQ71_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ0707654.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2654..3292
                     /locus_tag="WGQ71_001685"
     CDS             2654..3292
                     /locus_tag="WGQ71_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ0707655.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3744..4544)
                     /locus_tag="WGQ71_001686"
     CDS             complement(3744..4544)
                     /locus_tag="WGQ71_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ0707656.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(4680..5183)
                     /locus_tag="WGQ71_001687"
     CDS             complement(4680..5183)
                     /locus_tag="WGQ71_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707657.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(5520..6161)
                     /locus_tag="WGQ71_001688"
     CDS             complement(5520..6161)
                     /locus_tag="WGQ71_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ0707658.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMTMSEKSGVPLYFDRAL"
     gene            6490..8703
                     /locus_tag="WGQ71_001689"
     CDS             6490..8703
                     /locus_tag="WGQ71_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0707659.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRGELKKYNVHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAISKIRAYIRQQNAD
                     TVREEGRVQIDKQLAKLTPKPNLQELAENLGFKKLDDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT         2371 a         2374 c         1912 g         2211 t
ORIGIN      
        1 gggtatagcg gattaacaaa aaccagtacg gcgttgcctc gccttaactc aaagagaacg
       61 attctctaag gtgctgaagc accaagtgaa tcggtttcgt actatttgta ctgtctacgg
      121 cttcgttgcc ttgtcctgat ttttgttaat ccactataaa aattagaaat gcacattttc
      181 attattctcg cgcaggcagg actccagact tacccatttc agtaatgttt gaaaataaaa
      241 gaaaaatcag atgtttgtat tcccgcctgc gcagaaatgg agacggcgct ctgtcgtctc
      301 atttttgttt taatcaacta tatatagctg attaaacata agaaatgccg tctgaaagac
      361 tttcagacgg cattcgttca agcgtcgaac tttattgcgc cttggtttcg gttacaaaac
      421 cgattttggt gattcctgcc tgacgggcgg cttctaaagc tttgtttaca taatcgtatt
      481 ccaccgcctt gtctgccgca atcgccacaa tcacgttttc attctgctcc ttggcggctt
      541 tcagacggct ttccacttcc ccgatttcca ctttgcttgc agaatccccg ccgacataat
      601 agccgccgtt cgcatcaatc gtcaggcgca gggggtcttt aggctgtttg tcctgcttgt
      661 ttgtctgctc ggacgcggtc ggcagttcca aagggatgga atgcgtcagc accggcatag
      721 taatcataaa cacaatcagc aacaccagca tcacgtccac caacggcgta acgttgatgt
      781 cggacatcgg agaatcgtcg ccggaattca tcgaaccaaa tgccataatc agctatcctt
      841 ttgattaagc aggcggacgt gcaaatcgtg cgccatcgca tccaaatcct gggtcagtat
      901 ttttgtgccg cgattgagga agttgtatgc caacaccgcc ggaatcgcca cgaacaaacc
      961 cgccgccgtc gccaccagtg cctcgccaat cgggccggca accgccgcaa tactcatctg
     1021 cccgctttgc ccgatattga tcagggcgtg gtaaatcccc caaaccgtgc cgaacagccc
     1081 gataaacggc gcggtcgcgc cgatggaggc aagcgcggtc atcccgtaat caaaccggcg
     1141 cataatctgc gccatactgt tgcggatttg aatgaccaaa tactcgttca acggcaaagc
     1201 ctgcgccagt tcggacgctt cgtttcggcg gtagttgcgg taagactgca atgcctcttg
     1261 cgccagtttg gacaaaggcg catcgacggc gcgcactttt tcgaccgcgt cgttcagcga
     1321 caaagtatcg cgcatatgcc gtttgacggc ggcattccct ttgcgcgccc gatacagctt
     1381 gatgcagcgc aagacaacca aacaccacgt tacgatactc atcaacagca tcaacacaaa
     1441 cacaccgatc aggacgggat cgcccgattc aaatactaat ttcaaattca taatgattcc
     1501 aacactgaaa aaaccaatca aacatccaag ctgccgcaaa ccgctgcggc aaccgcctaa
     1561 ttcaattcaa acttgacggg gactttaaac tccgtccagg cattggcttg aaaatgcccg
     1621 ttttgcgccg ccttgcgtgc cgcattgtcc aaccgagaaa aaccactgct tttcacgatt
     1681 ttaacggact cgacatgacc gcccggagaa accaaaacgc tcaaaacaac cgtaccctgc
     1741 tcgtcattct ccatagaaag cgtgggataa gccgggcgcg gaatgctgcc gttggcgcgt
     1801 aaaggattgc ctttgctgct gccggctcct tccccgtgtt cgcctttgac accgccgcta
     1861 cctttaccgc tgccttctcc gcgccccgtt ccgtctcctt tggtaccggt tcccttatct
     1921 tccccattac cctgctcgct gtctgctttg gcagaagcat tgctgggatg ttcggcaggt
     1981 ttttcagacg gcttctcgac cggtttttcc gccggtttcg ggacaggctt cgcttccggc
     2041 ttaggctctg gtttcggttt ttcttcgggt ttcggctttt cttcaggttt cggctcttcc
     2101 ttaggctgct gaatatccgc atccgccttt ttcgtaacca ccggcttcaa aaccggcttg
     2161 ggcggctcga caggtttggg cggctcaggc tcaggttgcg gctcaggcgc agcaggcgca
     2221 cctgcgcctt cgggtgcacc gtcccctccg ccaaaatcac ccaaatcgac aaattcgata
     2281 acatttcctg actctatcac gggcagcttg tgcgcctgcc agagcaatgc caccattgcc
     2341 aaatgcagca gtgcgacgga aaacacgact gcgggggtta aaattcgttc tttatccata
     2401 attcgggcat aataatagca acaattccta tttgcaacct atttttacaa tttttggtca
     2461 tatgaatgtc tgttccgttc acaggcaaac cgtgtttaaa cgctgtatta cagcaaatca
     2521 tcagataacg ggccggcaga aaaaatgatt ccgtctgatt tcttattcca ataaaatcag
     2581 gttagatgat atattgccgc ttctgtctgt cagccgtttc gggctgcaca ccacatctgt
     2641 tcaaaggaaa accatgtttc aaaattttga tttgggcgtg tttctgcttg ccgtcctgcc
     2701 cgtgctgctc gccattaccg tcagggaggt ggcgcgcggc tatacggcgc gctactgggg
     2761 agacaacact gccgaacaat acggcaggct gacactgaac cccctacccc atatcgattt
     2821 ggtcggcaca atcatcgtac cgctgcttac tttgatgttc acgcccttcc tgttcggctg
     2881 ggcgcgtccg attcctatcg attcgcgcaa cttccgcaac ccgcgccttg cctggcgttg
     2941 cgttgccgcg tccggcccgc tgtcgaatct ggcgatggct gttctgtggg gcgtggtttt
     3001 ggtgctgact ccgtatgtcg gcggggcgta tcagatgccg ttggctcaaa tggcaaacta
     3061 cggtattctg atcaatgcga ttctgttcgc gctcaacatc atccccatcc tgccttggga
     3121 cggcggcatt ttcatcgaca ccttcctgtc ggcgaaatat tcgcaagcgt tccgcaaaat
     3181 cgaaccttat gggacgtgga ttatcctact gctgatgctg accggggttt tgggtgcgtt
     3241 tattgcaccg attgtgcggc tggtgattgc gtttgtgcag atgttcgtct gactggcttt
     3301 cagacggcat aaacgctcca gaaaacgcgg caggacatat tgccctgccg cgttttcctg
     3361 tagtgtaatc ttattttttt catcattatt agaaccaggt tgcatgataa tacctttcat
     3421 taactgaaac actgattaag aaactccagt ctgtctaatg atgaggtttt cacatcgcca
     3481 aaacttgcca atcaaatgct ggatttattg ccgtctgaga tttggtcaaa tccaaaggcg
     3541 acattcttag accctgtgtg taaatcaggg gtatttttgc gtgaaatcgt caaacgcttg
     3601 gatgaaggct tgaccaatca aataccagat aaacaaactc gcattaacca cattttaaaa
     3661 aatcaagttt ttggaagtac tgccacgtat gtaggtagct ttgaccgata tttgcataaa
     3721 aactcctttg ctggtgaaag gaattatttt gccaatttta aaatatttct ggcaccaaat
     3781 agtacaatga caaagacaat catgccaatg attaaatcag gatagctaga atgagtcaat
     3841 aacgtcaatg ctcccgccgc tatcacaccg atattgatga taatgtcatt ggatgtaaaa
     3901 atcatgctgg ctttgatatg gatttcttta ttttgatttt tgctcagtag atataagcac
     3961 agccagtttg caatcaatgc caaaaatgcc gtgccaatca tcagttgata attgggcagc
     4021 tgctcagcac cgataaaacg cctaatcact tctatcaccc caaataacgc caatattatc
     4081 tgcgttatcc ccgccaaaaa tgccacacgt tttttatacg ccagcgtcat accaatggct
     4141 gatagcgcca atatatagac aaagctgtcc gccagcatat ctagactatc agcaatcagc
     4201 cccatagaat tagcaaaaat accaaccgaa cactctatga taaaaaacac aaagttaatc
     4261 atgagcactt gatataataa tcttttttct aagtgctcat caggcttgtt aaacactatc
     4321 ttatcaacaa tcacttcggt ggaaatgata tgactatcaa aattaagcgg ttcaagtact
     4381 tgtaaaatcg ttgtatcttg attatcgtga tagacggtta agcaccgccc agcaatatca
     4441 aactgtaatt cataaatatc agacacatct tttaaacgca tgcgaatgag ctgttcttcg
     4501 gacgggcagt ccattttggt aatgttaaaa atggtctttt tcatctattt agttccttgt
     4561 tttgatcagg ttggctcaaa taaatctgtg tttatattgc tgcttggtaa tttttggatg
     4621 gtttgagtaa attgattagg ttaaaattta cctttggaag taccgccacg cataatagtt
     4681 tagatatgtt tataatctct ggataaaaaa acgtaataag tgcttactgg ataacaaagt
     4741 ccaaaccaat agcaggcaaa ataaggcatc cacccccctt cttcattaag gatatatatt
     4801 gagaaacaaa tcgcaactaa acagaaaaaa cttgggagat aaagccattt cattccccta
     4861 ttcaagaatc tagccaagat aggtattttg tattctacaa aaaagaaagg catttccaag
     4921 ggaaacatgt cagataaaaa cttttgttta ttttttacta tagatagaac cttgcttctc
     4981 aagagaaagc cattaataat accgatgaca gctattaata tatagagaat agtataagta
     5041 tgaataatct tcattagaca aaaagaagaa atggcagata aattacatac gatatattgg
     5101 aatataaaat atttacggtc taaaccttgt tcagttgcaa tttttttaaa attgccttgc
     5161 ataaaaaaat caaaggcgtc cattaaacta tctttcacat tagaaattta aaagctaaat
     5221 aatacgacaa acaatgtgaa gtactattca tggtttattt aaaaaataat actattctga
     5281 acattattta gatacagaaa ttaacaaatt agaactaaac aagcttttaa atactttaat
     5341 tttattggaa agctataaaa ggaactataa ctttacacac tagtcacttc tttttaagag
     5401 gcaaaaggga ttgggaaggt cgtcttggag ataagcactg gtatttcggc caatggtaaa
     5461 tagagtttac ctcaaatagg gtagaacctc cttcatctgt cagttaataa cagccacttt
     5521 tacaatgccc tgtcaaaata aagcggcacg cccgattttt cactcatcgt catcaaataa
     5581 cccatcacct tttggggcca ttcgatgccg cgcaccacgg tcagattcct caaaacgggg
     5641 aaaaccaaaa tatcctccat accgattccg ccgttgatgc cgtctgaagc accgtccatc
     5701 aaattttcca actcttgcaa atctgcgttt atccgttcga ggtattgggc ggttttattc
     5761 aaattggcgg aaaagctgcc gatgcttttc tcttttttgt ctgtaaaata tttcaccgct
     5821 tccggcgttg caaattcagg cagcccgatt ttgatcacgc gcggctgcac cagtttgtcg
     5881 ttgtatccgc ccaccttgtc cagccacgcc cgtatctcgg ggcggacttc gtctttcaga
     5941 cggtcttcgc ggtcgaaatg ccgcacaatg tccaaactct cgcccataaa cgaaccgtct
     6001 tctttttgca ggacgggcac ttgtttcgca ccgatcatac cgatcggcgt tgcctcgtcg
     6061 tcgtttgcca gcacggcttc ttcaacgtcc gcgccaaaca gcccggcagc catccgcgca
     6121 cgcacgcaaa acgggcaatg gtcgtaaata tacagtttca tcaaaatatt cctcgtcaac
     6181 ctgtcggtac cggctacctt aacaccccgc gccacccgaa acaagtttat cttcccgcct
     6241 atgcaccgta aataaataag ctgagacctt tgcaaaaata gtctgttaac gaaatttgac
     6301 gcataaaaat gcgccaaaaa attttcaatt gcctaaaacc ttcctaatat tgagcaaaaa
     6361 gtaggaaaaa tcagaaaagt tttgcatttt gaaaatgaga ttgagcataa aattttagta
     6421 acctatgtta ttgcaaaggt ctcaagctgt tacaataaac tcgtttttat cggaacggaa
     6481 gaccccatca tgaccgccat cagcccgatt caagacacgc aaagcgcgac tctgcaagaa
     6541 ttgcgcgaat ggttcgacag ctactgcgcc gcgctgccga acaacgataa aaaacttgtc
     6601 ttagccgccc gttcgctggc ggaagcacat taccccgccg atgccgccac gccgtatggc
     6661 gagccgctgc ccgaccactt cctcggcgcg gcgcaaatgg ttcatgaact cgacctgctc
     6721 cccgatgccg tcgccgccac cctgcttgcc gacatcggac gctacgtccc cgattggaac
     6781 ctattggttt ccgaacgctg caacagtacc gtcgccgagc tggtcaaagg tgtggacgaa
     6841 gtgcagaaac tcacccactt cgcccgggtg gacagcctcg ccacgccaga agaacgcgcc
     6901 cagcaagcgg aaaccatgcg gaaaatgctg ctggcgatgg ttaccgacat ccgcgtcgtg
     6961 ttaatcaaac tggcgatgcg tacgcgcacc ctgcaatttt taagcaacgc ccccgacagc
     7021 cccgaaaaac gcgccgtcgc caaagaaacc ctcgacatct tcgccccgct cgccaaccgt
     7081 ttgggcgtgt ggcagctcaa atggcagctc gaagatttgg gcttccgcca tcaagagccc
     7141 gaaaaatacc gcgaaatcgc gctgcttttg gacgaaaaac gcaccgaacg cctcgaatac
     7201 atcgaaaact tcctcaacat cctgcgcggt gaactcaaga aatacaatgt ccatttcgaa
     7261 gtcgccggcc gcccgaaaca catctactcc atttacaaaa aaatggtgaa gaaaaaactc
     7321 agcttcgacg gcctctttga catccgcgcc gtgcggattc tggttgatac cgtccccgag
     7381 tgttacacca cgctgggcat tgtccacagc ctttggcagc ctatccccgg cgagttcgac
     7441 gactacatcg ccaacccgaa aggcaacggc tataaaagtt tgcacaccgt catcgtcggt
     7501 cccgaagaca aaggtgtgga agtgcaaatc cgcaccttcg atatgcacca attcaacgaa
     7561 ttcggtgtcg ccgcgcactg gcgttacaaa gaaggcggca aaggcgattc cgcctacgag
     7621 caaaaaatcg cctggttgcg ccaacttttg gactggcgcg aaaatatggc ggaaagcggc
     7681 aaggaagacc tcgccgccgc cttcaaaacc gagctgttca acgacacgat ttacgtttta
     7741 accccgcacg gcaaagtcct ttccctgcct acgggcgcga cacccatcga cttcgcctac
     7801 gccctgcaca gcagcatcgg cgaccgctgc cggggcgcga aagtcgaagg gcaaatcgtg
     7861 ccgctgtcca ccccgcttga aaacggacag cgcgtcgaaa tcattaccgc caaagaaggg
     7921 catccttccg tcaactggct gtacgaaggc tgggtcaaat ccggcaaggc aatcagtaaa
     7981 atccgcgcct acatccgcca gcaaaacgcc gacaccgtgc gcgaagaagg ccgcgtccaa
     8041 atcgacaagc agcttgccaa actcacgccc aagcccaacc tgcaagagct tgccgaaaac
     8101 ctcggcttca aaaaactcga cgacctctac actgccgtcg gacaaggcga aatttccaac
     8161 cgcgccatcc aaaaagcctg cggcacgctg aacgaacctc cgcccgtacc cgtcagcgaa
     8221 accaccatcg tcaaacagtc caaaatcaaa aaaggcggca aaaacggcgt gctcatcgac
     8281 ggcgaagacg gtttgatgac cacgcttgcc aaatgctgca aacccgcgcc gcccgacgac
     8341 attgtcggct tcgttacccg cgatcgcggc atttcggtac accgcaaaac ctgcccctct
     8401 ttccgacacc tcgccgaaca cgcgcccgaa aaagtactgg acgcaagttg ggcggcgttg
     8461 caggaaggac aagtgttcgc cgtcgatatc gaaatccgcg cccaagaccg ctccgggctt
     8521 ttgcgcgacg tatccgacgc gctcgcccgc cacaaactca acgttaccgc cgtgcaaacc
     8581 cagtcccgcg acttggaagc cagtatgcgg ttcacgctcg aagtcaaaca ggtcaacgac
     8641 ctcccgcgcg tcctcgccag cctcggcgac gtcaaaggcg tattgagcgt tacccggctt
     8701 taaatacaaa aatgccgtct gaaagccgta taacgcttca gacggcattt tgattgacgg
     8761 ggtttgctat tttttgttgc atagtcaatt aaaaacaaaa tagtacaata ctcaactttg
     8821 aaggtctaac catggcatac tctgcggact taagaaacaa agctttaa
//
LOCUS       DAWFIE010000076         8711 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWFIE010000076 DAWFIE010000000
VERSION     DAWFIE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.076"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(86..2878)
                     /gene="polA"
                     /locus_tag="WGQ71_001690"
     CDS             complement(86..2878)
                     /gene="polA"
                     /locus_tag="WGQ71_001690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ0707660.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKAIAPEKLDYQA
                     VTTEAQFAALLDKLSRADTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPTLKKIGQNLKYDQHVFANYDIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QQAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGIAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNISAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVRNWLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
     gene            complement(3024..3530)
                     /gene="luxS"
                     /locus_tag="WGQ71_001691"
     CDS             complement(3024..3530)
                     /gene="luxS"
                     /locus_tag="WGQ71_001691"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ0707661.1"
                     /translation="MPLLDSFKVDHTRMHAPSVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYLMHSLSEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            complement(3552..3971)
                     /locus_tag="WGQ71_001692"
     CDS             complement(3552..3971)
                     /locus_tag="WGQ71_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ0707662.1"
                     /translation="MAQLPLYRTAEIENFTVGTPEVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKST"
     gene            complement(3997..5013)
                     /locus_tag="WGQ71_001693"
     CDS             complement(3997..5013)
                     /locus_tag="WGQ71_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ0707663.1"
                     /translation="MNTRPFYFGLIFIAIIAIFANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            complement(5040..5363)
                     /gene="cyaY"
                     /locus_tag="WGQ71_001694"
     CDS             complement(5040..5363)
                     /gene="cyaY"
                     /locus_tag="WGQ71_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ0707664.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWNFDCRFAGNVLTIEAEN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDGRDFYDVLNEALSAASGEAV
                     KIAEL"
     gene            5434..5604
                     /locus_tag="WGQ71_001695"
     CDS             5434..5604
                     /locus_tag="WGQ71_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0707665.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSTPQSAPQTQK"
     gene            5615..6859
                     /gene="lysA"
                     /locus_tag="WGQ71_001696"
     CDS             5615..6859
                     /gene="lysA"
                     /locus_tag="WGQ71_001696"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ0707666.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTASVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQNEKVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVEAKNISPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGGGYRLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(6895..6987)
                     /locus_tag="WGQ71_001697"
     CDS             complement(6895..6987)
                     /locus_tag="WGQ71_001697"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ0707667.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(6984..8519)
                     /locus_tag="WGQ71_001698"
     CDS             complement(6984..8519)
                     /locus_tag="WGQ71_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0707668.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
BASE COUNT         2113 a         2276 c         2257 g         2065 t
ORIGIN      
        1 atccactata ctataggaac atttagaaaa aatataccaa aaattaaatt ttatttaaat
       61 aaagatagca tataacacct ttcaatcaat gtgcctcttc ccaattctcc cctacgccaa
      121 cctcagccac cagcggtaca tccaataatc cgccgtccac tttcgccata atctgcggca
      181 gtttttcttt gacaaaatcc agttcggttt caacgacttc cagcaccagt tcgtcatgca
      241 cctgcatgac cagtttgctg ccgatgccgt ctgaaagcca gtttcgcacg tctatcatgg
      301 cgcgtttgat gaggtcggac gcggtgcctt gcatgggagc gttgatggcg gcgcgttcgg
      361 ctcctgcgcg ggcgttggcg tttttgttgc ggatgtcggg caaatagagc cttctgccga
      421 acagggtttc gacgtagcct tgggcggcgg cttgttcttt ggtgcgctgc atgtattcgg
      481 cgacgccggg gtagcgggcg aagtagcggt cgataaagtt tttagcggag atgttgtcga
      541 tgcccaatga tttggcaagg ccgtattggc ccatgccgta aatcaggccg aagttgatgg
      601 ttttggcgta gcggcgttgt tcggacgaga cgttttcggg ggcgatgccg aacacttcgg
      661 cggcggtgcg gcggtgtacg tcttcgccgt tttggaacgc ggcaatgagg gttttatcgc
      721 cggagaggtg cgccatgatg cgcagctcga tttgggaata gtcggcggaa acgatgacgc
      781 tgccttgcgg tgcggtgaag gcgcggcgca cgcgtcggcc ttcggcggtg cggatgggga
      841 tgttttgcag gttggggttg ttgcttgcca agcgtcctgt aatggcgacg gcttgggcgt
      901 aggtggtatg cacgcggccg tccttggggg aaatcatttc gggtagtttg tcggtgtagg
      961 tggatttgag cttcgccagg ctgcggtttt gcaggatgat tttaggcagg gggtagtcgg
     1021 gcgcgagctg ttcgagcacg gcttcgttgg tggaaatgcc gcctttggcg gtttttttca
     1081 ggcctttggt ggggatgccc attttgtcga acaggatttc ttgcagctgt ttgggcgaat
     1141 tgaggttgaa cggctggcct gcggcggcat aggcttgttg ttcgagcttc atcagctcgg
     1201 cgccgagttc cgcgctttgg cgggcgagtt cggcgcggtc gatttgcacg ccgttgcgtt
     1261 ccatttcaaa caatacctgc gcgacgggca gctccatttt ttcatacatt tcaagctgtt
     1321 tttcgtccat ttgcgcgcgc agatgcgctt cgaggcgcag ggcgaaatcg gcgtcttggg
     1381 cggcgtattc ggtcgcctgc ccgatagcga cgtcggcaaa accgatttgc ttcgcgcctt
     1441 tgccgcacag cgattcgtag gtaatggttt ccaagcccag ccagcgttcg gacaattcgt
     1501 ccaaaccgtg tccgagatgg ctctcgatga tgtaggaagc gagcatggcg tcgccggcaa
     1561 tgccgttcag ggcgatgtcg tagttggcaa aaacatgttg gtcgtatttg aggttttgcc
     1621 cgattttttt cagggtgggg ttttccaaat gcggtttcag acggcctaat acgtcttgca
     1681 aatcaagctg ttcaggcgcg gcggtcaggc tgtggcctac ggggatgtaa accgcttcgc
     1741 ctgcttggaa ggcgatgctg atgccgacca gcgaggcatt catcgcatct aatgacgtgg
     1801 tttccgtatc gatgccgatt gtgtccgccc gcgacagttt gtccaacaaa gcggcaaact
     1861 gcgcttcggt ggtaacggct tgataatcca gtttttcggg ggcgatggct ttttcggctt
     1921 gtttttcaaa cggcatttcc gcattcaaag ccgcctgctc gccgatgctg tcgctgccga
     1981 acaaatcatc ggtcgagccg gtattcatgt ttgattccgc ttctttcagc cacgtgcgga
     2041 agccccagcg tttaaaatcg acaaccagtt gcgcccattt cggcgtggta cggcgcaggc
     2101 tttcgatgcc gtctgaaagc tcggcgtgca agtccacatc ggttttaatc gtgaccaaat
     2161 catacgacag cggcagttgg ggcagcgcgg cttgcaggtt ttcgcccact ttgcccttga
     2221 tttccgaagc gtgttctatc acgccttgca atgagccgta ggcttccagc cacttcaccg
     2281 ccgttttcgg gccgcatttt tccacgcccg gcacgttgtc caccttgtcg cccatcagcg
     2341 cgagataatc gcggatttgg tcggggcgca cgccgaattt tgccttcacg ccttcaatgt
     2401 ccagcgtttc gctgctcatc gtgttcacca gcgtaacgcg ctcatccacc aactgcgcca
     2461 tgtccttatc gccggtcgaa acaatgactc gcaaaccatg ttccgccccc tgtttcgcca
     2521 gcgtgccgat cacatcgtcc gcctccactt gcccaatcac caataccggc cagcccgtca
     2581 ggcgcactaa atccggcagt gcttccgcct gcgggcgcaa atcgtcgggc atcggcgggc
     2641 gcgtcgcctt gtattcttca aacatttgat ggcggaaatt tttgcctttc gcatcaaaaa
     2701 ccaccgcgca ataatcgtgc ggatattccg accgcaaacg gcgcaacata ttcaatacgc
     2761 catacagcgc acccgtcggc gcaccgtcgg gtgcggtcag gttttgcccc atcgcgtgat
     2821 acgcgcggta gagataggaa gagccgtcaa caaggaggag tgtaggtctg ttggacataa
     2881 aaaacccgct taaaaccgat aagggacaga aaaaaatagg gaaggacgat gccggcttaa
     2941 aacccgcccc cgcacaaccc cggattataa cggaaaaggc aaatgccgtc tgaaaccctt
     3001 gttcagacgg catttttggc ggattaggcg ttcagcagcc cttcatccag cgtcagctct
     3061 tcatttttgt tcaccgccac tttgcgcgcc aacacgtttt gcgcgatttg ttgcgcttcg
     3121 gagagcgagt gcatcagata agtgccgcat tggtattcgt tcaactcggg gattttgctt
     3181 tggtctttga cattcaaaac atcctgcatc gaagccagcc acgcatcggc gacctgctgt
     3241 tcggaaggcg tgccgataag gctcatgtag aaaccggtgc ggcagcccat cggggaaatg
     3301 tcgatgattt ccacgccgtt tccgttcaag tggtcgcgca taaagcctgc aaacaaatgc
     3361 tccagcgtgt gtatgccttt ttcgggcagg atttctttgt tgggaacgca aaagcgcagg
     3421 tcgaatacag taatggtgtc gcctttgggc gtggtcatgg ttttcgccac gcgtacggag
     3481 ggggcgtgca tacgggtgtg gtcgactttg aaactgtcta gtaggggcat ttgggtatcc
     3541 ttttgtgagg gttatgtaga ttttcgggat aggattttgt ccagcaattc atcatccaag
     3601 acgcggtttt cgactttcac ccattcgccg cctgcttcag ggaagccgtt gcggcaatat
     3661 cctgcctgtt cgacaaaatc atagacggca tggatgtagt cgttgataca caatgcggct
     3721 atggtgcagg catcatccca taaaatcaag acatgattgg ggatatgttt gtcggataca
     3781 tcttcgacat tgtaaatgtg caaggcatct aaaatcccgt cttgcgaagc ggcatagaag
     3841 tagcctgtgt cgccgtcgtc ttcaaagaca acaccataag ggatatgttt ggaaaatgat
     3901 tctaaaactt cgggcgtgcc gacagtaaaa ttttcgattt cggcagtccg atacaggggt
     3961 aattgtgcca ttgggtaacg ctctctttag tgaattttat attaattttg atattccata
     4021 gttcactaaa aatccaccaa ccactagcca taaataagtt agtattccta aaacaaacgg
     4081 ttttaatcct gcctttttga ttgcgcttgc ttgcgtcgtt aagccaagcg cagccattga
     4141 tgaaattaat aagaaagaat cgatttcaac gaataatttc acgagttctt ttggtaataa
     4201 atcaaaagaa ttaaaaatgg caacaccaat aaaaagtaca gcaaaccaag gaattgtaat
     4261 tttgtgtgat gtattttctg atactccatt actacgtgtt aataaccaag aaagcattaa
     4321 taaaaagggg gcgagcatca tcactcggat cattttggaa atgacggcag tattcgccac
     4381 gataggatca atattttccc caatcgcata aacttgagcc acttcgtgta cactagaacc
     4441 aacataaata ccgaattgat gggcgttaat taaatgttgt gaccacgtgt agaacaaggg
     4501 gtaagtaaaa atagcaagcg tcccgaaaat gaccactacg gcaatcgcca ctgaaacttt
     4561 atgggattct gctttggtaa caggctctgc cgccatcact gctgccgcac cgcaaatgct
     4621 gcaacctgcc ccagtgagat aaaccagttg tttatccatt tttagataac gaatgcctaa
     4681 aagtgcggta aaaaagaagg ttgaaattag catgattgca tcagtgacga cagcatttaa
     4741 tcctacatcg gcgatatcgc caaaagtaag gcgaaaacca tacagcacaa tgccagtgcg
     4801 aagaagcgcg cctttggcaa acaaaacacc tttttccact tgtgttgaaa attgcggata
     4861 aatggtattg ccgattgcca ttcccagcaa gatagcaata attaaagcac tgatatgata
     4921 atgatgggaa aaatcagtgt ttcctaaata gttagcaaat atagcgataa tcgcgataaa
     4981 tataagtccg aaataaaagg gacgtgtgtt cattttttct ctccgtgata acactcaaat
     5041 tacaattcgg caatctttac cgcttcgccc gaagccgcgc tcagtgcttc gtttaaaacg
     5101 tcataaaaat cgcgtccgtc tcgcgttgcc agccatttgc cgttttgctc ggcgaaatgg
     5161 tagccgccgc ttttggcggc aatccacaat tcctgattgg gcgtgtggcg gttgacgatg
     5221 atttgcgtgc cgttttcggc ttcgatggtc aggacgtttc cggcaaaccg gcagtcgaaa
     5281 ttccagccgt tttcgtcgat ttggtcttcg atgtgttcaa ataatgcttc gctcgcgcgg
     5341 ataaactcgc tttcggtcat catagctttt tgcgtgtttt ttgtttaaga tgccgaatct
     5401 tgccacattc gcgcttatga aggaagttta ccgatgaaat acggcgtatt ttttgcggcg
     5461 gcaaccgccc tcctgctttc cgcctgcggt tacaaaggcg acctctactt gcccaaagaa
     5521 ggcgacaagg cgcgtttcgg cgtaatccaa accggtttgc agcttcaaag cacaccgcaa
     5581 tccgctccac aaacccaaaa atgaaaacga aaatatgacc ctattttgcg aacaagtccc
     5641 ctacccccgc cttgccgaag cattcggcac gccgctttat gtgtacagcc aatccgcgct
     5701 gaccgaagcg tttgaacact accaaaccgc gtttgccgct ttgaacccgc tcgtctgtta
     5761 cgccgtcaag gcaaacggca atctgagcat tatcaaacac ttcgcctcgc tgggcagcgg
     5821 tttcgacatt gtgtccggcg gcgaattggc acgcgttttg gcggcaggcg gcgacgcggc
     5881 gaaaacgatt ttttccggtg tgggtaagag tgaagcagaa attgagtttg ccctgaatgc
     5941 gggcgtgaaa tgcttcaata tggaaagcat ccccgaaatc gaccgcattc aaaaagttgc
     6001 cgcgcgtttg ggtaaaaccg cgtccgtctc cctgcgcgtc aatcccgatg tcgatgcgaa
     6061 aacccatcct tacatctcca caggtttgaa agccaacaaa ttcggtatcg cctacgccga
     6121 cgcgctcgaa gcctaccgcc atgccgcaca acagcccaat ttgaaaatca tcggcatcga
     6181 ctgccacatc ggctcccaac taaccgactt aagcccgctg gtcgaagcct gcgaacgcat
     6241 tttgattttg gttgacgcgc ttgccgccga aggcattgtt ttggaacatt tggacttagg
     6301 cggcggcgtc ggcattgttt accaaaacga aaaagtgcct gatttgggcg cgtatgccca
     6361 agcggttcaa aaactgatcg gcacacgccg tctgaaactc attcttgagc ccggccgcag
     6421 cttggtcggc aacgcaggtg cgttgttgac gcgtgtcgaa ttcgtcaaac acggtgaaga
     6481 gaaaaacttt gtgatggtcg atgcggcgat gaacgatttg atgcgcccgg cactttatga
     6541 tgcctaccac cacatcgaag cagttgaagc caaaaacatc tcgccgctga ccgccaacat
     6601 cgtcggccca atttgtgaaa ccggcgactt ccttggcaaa gaccgcacca tcgcctgcga
     6661 agaaggcgat ttgctgctta tccgcagcgc gggcgcatac ggagccagta tggcgagcaa
     6721 ttacaacgcg cgcaaccgtg cggcggaagt attggttgac ggcggcggat accgactcat
     6781 ccgccggcgc gaaaccttgg aacagcaaat ggcaaacgaa ctcgcctgcc tgcaagccga
     6841 acatcaaaat gccgtctgaa gcggttcaga cggcattttt aacgctctaa aggcttactc
     6901 gttcggcagc cttaacaggg aaagcagcag ccctccccag attatcacga ttgagacaat
     6961 catcatcaca atggcggaag tactcatttt tcttctcctt gttcatgttc gtgttcgtct
     7021 ttgacgttga aatcctgacc gtgtttccaa ggcagcaacg acagcagcag cccgaacacg
     7081 accaacgccg ccgacattcc ccagccgaaa atactgagga aaccatccgg ataaccttcg
     7141 taatttttct ccatcaggcc gctggtatct ttaaacagca tatagccgag catcacgacg
     7201 gtaaccacga cgcagaccgt ccacaagccg ccgatgcgga tggaggacaa agcgttcagg
     7261 tgcttgcgta attccggcag cctgccgctg atgatgatgg cggcaacata aacaaagccg
     7321 gcggcaacaa tgccgtaggt gttgacgaat ttgtccatca cgtccaaaac cggcagcccc
     7381 gtcgccgtac cgaacagcag cgtggaaaca atgcccatcg gaatgcagac cagcagcgtg
     7441 gcgttgacgc gcccgatgtt cagcttgtcc tgaatcgccg ccacaatcac ttcaaggatg
     7501 gaaatcatcg acgtaacgcc ggcgaatacc agcgaaccga aaaacaatat gccgatcagc
     7561 cagcccatcg gtgcctggtt gataatggtc ggaaaggcga taaacgccaa accgatgccg
     7621 cctgaggcaa cctcgttgac cgccttaccg cccgcctgcg ccataaagcc caatgcggca
     7681 aacacgccga tgcccgcgag cagttcaaag ctgctgttgg caaaaccgac caccagcccc
     7741 gttccgccca agtcggtttt tttcttcaaa taagaagaat aggtaaccat aatgccgaag
     7801 cagatggaaa gcgaaaagaa aatctgcccg tatgccgcca cccagacctt ggaatcggcg
     7861 agtttcgacc agtcgggcgt aaacaatgcg tccaagccct ttgccgcgcc cggcaacgtc
     7921 agcgaaatgc cgaccataat caaaaacatc accaaaagta gcggcataaa gaacgacgag
     7981 gcgcgcgcca cgcccttttg cacgcccaaa gccataatgg cggcggtaaa aacccacacg
     8041 cccgccaaag gaccggcgac tttgccgaca aaatccaaac ccaaggcttc cgggcccgcc
     8101 atttgcagga agtccttaaa gaaaaaaccc tgcggatccg caccccaggc ggcgttgacc
     8161 gaataatagg tatagcttgc cgcccaaccg ataatcactg cgtaatagat gcagatgacg
     8221 atattggtca tcacgttcca ccagccgacc ggctcaaacc agcgtccgag gcggcggaaa
     8281 gccaagggcg cagaaccacg gtaacggtgg ccgatggcat aatcgagcag cagcagcggg
     8341 atgcccgccg tcagaagcgc gaccagatag ggcaggatga acgcgccgcc gccgttttca
     8401 aaagcaatat aggggaaacg ccaaatattg cccaagccga cggcggaccc gatggcggca
     8461 atcataaacg cgcggcgcgt gccgaatgtg gcgcgttctt tcgtcttgga atcagacacg
     8521 ttgatacctc ttgaattatt tattaaaaac aagccgttat aggcgaaata tatagaaaca
     8581 gcgtctgaca gggataatgt cttctgaaaa aaacaaaccg gcttgtgacc gattgtttac
     8641 aatctatgtg cttatcgtaa aaaaatttaa cgctgatggc aagcggtgaa ggtttgaaaa
     8701 cgaaaaattt t
//
LOCUS       DAWFIE010000077         8623 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWFIE010000077 DAWFIE010000000
VERSION     DAWFIE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8623
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.077"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(274..2964)
                     /gene="glnE"
                     /locus_tag="WGQ71_001699"
     CDS             complement(274..2964)
                     /gene="glnE"
                     /locus_tag="WGQ71_001699"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ0707669.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFIYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKALVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPTSPKQRQLLAESMGFDSYSAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTSEIQTAFDRIRTEILTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARKYPQLLDNYGNIALLNIAADCSLIDKNLAEQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWRDVFGEEAATA"
     gene            complement(3182..3364)
                     /locus_tag="WGQ71_001700"
     CDS             complement(3182..3364)
                     /locus_tag="WGQ71_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707670.1"
                     /translation="MGFWNGVAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIVKSD
                     GFLKIPHGRKVRLMLF"
     gene            complement(3501..3974)
                     /locus_tag="WGQ71_001701"
     CDS             complement(3501..3974)
                     /locus_tag="WGQ71_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ0707671.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4111..5136)
                     /locus_tag="WGQ71_001702"
     CDS             complement(4111..5136)
                     /locus_tag="WGQ71_001702"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ0707672.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNMVYKLGLIPVTGNRTKPV
                     KLMGLDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPNPQPGNPQPRLFRVPE
                     YQGIINRMGFNNHGIDAMIQNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGRDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     RSAQNAV"
     gene            5368..5604
                     /gene="acpP"
                     /locus_tag="WGQ71_001703"
     CDS             5368..5604
                     /gene="acpP"
                     /locus_tag="WGQ71_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ0707673.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            5760..7007
                     /gene="fabF"
                     /locus_tag="WGQ71_001704"
     CDS             5760..7007
                     /gene="fabF"
                     /locus_tag="WGQ71_001704"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ0707674.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            7312..8446
                     /locus_tag="WGQ71_001705"
                     /pseudo
     CDS             7312..8446
                     /locus_tag="WGQ71_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT         2012 a         2256 c         2298 g         2057 t
ORIGIN      
        1 cgtctgaaaa gtagaatgcg ctattttaat ggaaacggcg gggttttaaa cggttcttat
       61 ttgtttgttt tgatttttgc ttgggtaaaa aatcccatca ttcccacaaa aacagaagcc
      121 tgaaatcccg tcattcccgc gaaagaggaa tccggttttt gggtttcagc cattcccgat
      181 aaatcgcttt agctctgccg caaccttaaa acggcacggt acgaaaatac cgtctgaaac
      241 ccagattgtc aggcttcaga cggcattttt tgttcaggcg gttgccgctt cttcgccgaa
      301 cacgtcccgc cacaatttcc tgacattgcc gtaatgtgcc aacagttcgc cggttacttc
      361 ggtttttgcc gcgtcgcgca gtttggtgtt gtgctgctgc cggcggtaga agcggtaggc
      421 ggtgcggctt tgttcggcga ggtttttgtc gataaggctg cagtcggcgg cgatattcaa
      481 gagggcgatg ttgccgtagt tgtcgagaag ttgcggatat tttcgggcat gggcaaggat
      541 gaggtattgg acgataaatt cgacatcgac cacgccaccg cgcgcgtatt tgacgttgct
      601 gtcggcaggc gggtgggtgg ggaacatttt ttcgcgcatt tcgatgattt cgcctgccaa
      661 ggcggtttgg tcgcgttcgg cggtaaggat ttcggtgcgg atgcggtcga aggccgtctg
      721 aatttcggac gtgccgcaga tgaagcgggc gcgggtaagg gattggtgtt cccacgtcca
      781 ggcgttttcg cgctggtatt tttcaaaggc ggcgatgctg tgggcgagga aaccggcgtc
      841 gccattaggg cgcaggcgca ggtcggtttc gtagaggctg cctgcgccgg tggcggcgga
      901 aagccagttg gtcaggcgac gggcgaggcg gctgtacacg tcgcctgcgt cggggtgggg
      961 atcgtcgtag agatagacga ggtcgaggtc ggaggcgtag ccgagttctt tgccgccgag
     1021 tttgccgtaa ccaacgacgg cgaattgcgg ggtgtcgcgg tgttttttgg gcatgtccgc
     1081 ccatgcgcac agcagggcgg cggcgaggat ggtgtcggcg agggcggaga gttggtcgga
     1141 gagggattct accgtccaca gtccggcgag gtcttggacg gcgaggcgga agacttgggc
     1201 gtgctggaag cggcgcaggg tgtccatttg cgcttcagta tcgccgccgc aggctttgag
     1261 gtcgtctgaa agggcggcgg cgagtgcctg ccaatcaaac gcggtatcca aaagctgcgc
     1321 gctgatgagt tcgtccaaca aaatcggata tttgttcaga tacgccgcca cccaagaact
     1381 ttggcccata atctgcgcca gttgcgccaa ggtttgcgga tgttcgttga ggaaggcgag
     1441 ataggcggat cggcggctga tgttttcgag aaaatccaac agccgcatca atgtatcggt
     1501 cgggttgctt tgcgctgccg ccgcctgtac gaacagcggc acaaccgcat cgaaacgcgg
     1561 ctgggcgtgt gcggaaagat ggcggtattt atggccgtgg cggatttggt cgagccttgc
     1621 ggcgacggtt tcggcatcga acccgtgcgc cttcagacgg catcgccgcc cttcttcgtc
     1681 gggtttgtcc tgccatgccc attgccattc gctgttgtct tgcgtttgct cttcgggttc
     1741 gctcaaaatt tcgttgaaca actgattgac tttgttccga tgaacattga gaccgtctga
     1801 aaaagcggaa taactgtcga aacccatgct ttcggcgagc agttgccgct gtttgggcga
     1861 ggtcggcagg gtttgggttt gccgatcgtc ccagtattgc aggcggtgtt cgacatcgcg
     1921 caggaatcgg taggcggcaa gcagagtttc gacgttttca gacggcatga tcccggtttc
     1981 ggcaagtttc ttcagcgttt cctgcgtgcc tttcagttgc agcgcacgca tttggccgcc
     2041 gcgtatcatc tggaaaatct gggcgataaa ttcgacttcg cggatgccgc ccgcgccgag
     2101 tttgatgttg tccgccatgc cttttttgct gacttcgctg cggatttggc ggtgcagctt
     2161 acgcatcgcc tcatacgcgc cgtaatccag atatttgcgg aacacaaagg ggcgcaccag
     2221 tgctttgatg tcgttcggat acggcgtaac cacgcgacct ttgcaccacg cgtagcgttc
     2281 ccattctcgc ccctgtgtaa tcaaatattg ctccagcgcg gtttcgctca ataccaacgc
     2341 gcccgaatca ccgtccggcc gcagccgcat atcgacgcgg aacacctgcc cgtcggcggt
     2401 gatgtcgttc agcagcgcaa tcagtttctg cccgactttg gtgaaaaact cctgattgcc
     2461 ccgttcgcgc ctgccgtcgg tgtcgccaga ttcgggatag atgaaaatca aatcgatgtc
     2521 ggaagacacg ttcaactcat agccgcccgc cttgcccatc gccaccacgc tcaaatgctg
     2581 cggcgatttg gtataacgcc cgatcggcgt gccgtacatg tcccgataat aggcgtaggc
     2641 aaaatccagc gcggtattga cggcaaaatc ggcaaacagc gtaatcgtgc gggttacttc
     2701 gttcaaatcg ctgatacggt tgatatcgcg cacgataatc tgcgacacca cataacggcg
     2761 caacccgcgc aactgccgcg ccaattcttc ctcgttttct tccgcacgga ttttgtccca
     2821 gtcggcaaag gcttggaaat ccgcttcggt caaaaccttg tccagcatag gcaggaaaat
     2881 ttcgggcttg agtttgccgt tgtcgagctg gcgggcgagg aagagggaat ggcggcgggc
     2941 ggtgtcgagg cggttgtcgg acatttcgga ttccgtttga aaaaatggca ggaagatgat
     3001 ggcggattca cttcaaatca ggacagggcg aggggatgcc gtaccggttt aaagtcaagc
     3061 cactatatca tagcaacctt cgatgccgtc tgaaaccttg ccccgacatc cagacggatg
     3121 cctgccgata aaaaacagcg gcaaatgccg ccgtttcctg ttcacacctc gccacgctct
     3181 tttaaaacag cataagccgc acttttctcc cgtgtggaat ttttaaaaaa ccatcactct
     3241 tgacgatttc atgcagctcc tcacttgatt tctccgcata ttccattttc aacgccttat
     3301 gctcattgcc ggcttcaatc attccctctc ccactgcttt tgccacacca ttccaaaaac
     3361 ccataatcat gtcctccaat cgtttcaaat aaataaggtc gttgtttctg tcaagaaaca
     3421 acgacagttt ataaactgtc ggaaaacaaa atgccgtctg aaggccggat aatgcttcag
     3481 acggcatcgg attgccggtt ttaaccctgc cctccgccgt tgttcaaata gtgttcccag
     3541 tgagaatata accgggcaat attttcttca taggtgtggg cagaaggaat ataaccgtct
     3601 ttgggcggca tatacctgac aaatgattct atttcttccg gataatcaaa atctgcataa
     3661 attttttcaa cttcccccaa aggatcctca aattcttctt ttctatggaa tacatcgctt
     3721 aagaggacgt acagccaatt tttaggggaa taatccctca tttccgaagc caaatcttcc
     3781 agaacaggct ttaattcaag aatattggat ttgttgatta aagatagttt gaacacacgt
     3841 tcatcatgat ctgaaagcgt catttttcgg gcataagctg ccacatcagc ccaacccaag
     3901 tatttattcc cataccccca cagaatatcc ttccaagaaa gacggacttt ttgcgcggtt
     3961 aaatcaaggt tcatttttta ctccggttac ctgctccgtg ttcacgcatc ggtttgcctt
     4021 atttcaaaca gggcggcgcg taagggattt tcaaggggcg gcaggcggaa aaaggaaatg
     4081 aaaatgccgt ctgaacggca aaacgtgcgt tcagacggca ttttgggcgg atcgcgccaa
     4141 agccttcaaa cattctttga ccaatgccgg acctttgtat atcaatccgc tgtacacttg
     4201 gacggcggtc gcgcccaagc ggattttctc tgccgcgtcc ctgccttcca taatgccgcc
     4261 tacgccgata atcggcagct tgccgtcgat gtggtctgcc aacagcttca acacccgatt
     4321 acttttttca tgaacgggca gcccgctcaa accgccctgc tcgcctgcga gcggatggct
     4381 gccgagactt gatttgtcga tggtggtatt ggtagcgatg atgccgtcca tttcgacgga
     4441 tttgacaacg tgggcgatgt cttcgatttg tgcttcatcc aaatcggggg cgattttgac
     4501 ggcgagcggg acgtatttcc cgtgtacaga ggcaagctgt gcctgtttgt ttttcaaagc
     4561 ctcaagcaat gcgctcaact cgtcgccacc ttgcagcgcg cggaggtttt tagtgttggg
     4621 cgaggaaata ttgacggtaa tgtaacttgc gtgtgcgtag gctttttcga ggcagattaa
     4681 ataatcatcg gcagcgtttt caatcggtgt tacagcgttt ttgccgatgt tgatgcccaa
     4741 tacgccgctg aatttacttt tttcgatgtt ttgaatcatg gcatcgatgc cgtggttgtt
     4801 gaagcccatg cggttgatga tgccttgata ttcgggaacg cggaagaggc gcggctgcgg
     4861 gttgccgggc tgcgggttgg gcgttaccgt gccgatttcg atgaaaccga agccgagcgc
     4921 gcccaatgcg tctatatatt cgccgttttt atcgagtccg gcggcaagcc cgacagggtt
     4981 gggcaaatcc aaacccatca gttttacagg tttggtacgg ttgccggtta cgggaatcag
     5041 acccaattta taaaccatgt tgagcgcgtc gagcgtgaag tggtgggctt tctcggcatc
     5101 gagtgcaaac aggatgcggc gggcaagtgg atacatgaca gggctttctg cttcagatgg
     5161 aaatgccgtc attttaaccg aaatcccgtc aaacatacgg cacacaggtc aaacttgaac
     5221 gacaaaaccc gccgcaccca aaaatgtaca aatcggcaaa tattgatata atagtcatta
     5281 gatagaaaac tctattaaaa gtcggtacaa taccaccgat ttatttccct taaacaacct
     5341 gccgcaaggc ataaaggaac gactgatatg tcaaacatcg aacaacaagt taaaaaaatt
     5401 gttgctgaac aactgggcgt aaacgaagcc gacgtgaaaa acgaatcttc tttccaagac
     5461 gacttgggcg cggattcttt ggacaccgtg gagctggtta tggctttgga agaagcattc
     5521 ggctgcgaaa tccccgacga agatgccgaa aaaatcacca ccgtccaact ggctatcgac
     5581 tacatcaatg cccacaacgg ctaaccggtc gtcgcccgac acaacagcct ctgctgcacc
     5641 gcgcaataga ggcttttccc ttatatggca aactgtttga acccctgccg caaattacag
     5701 acggcatctg cacgacagcc gcatatgccc ccattccgac aaccaacagc gaggttatca
     5761 tgagtcagag aagagtagtc attacaggct taggtcaggt ttcccctgtc ggcaacactg
     5821 tcgcagaggc ttgggacacc ctgctcgccg gcaaaagcgg catcggcgcg attacccgct
     5881 ttgacgcatc cgacatcaac agccgtgtcg ccggcgaagt gcgcggtttc gacatcggac
     5941 aatacatcag cgcgaaagaa gcgcgccgga tggacgtatt catccactac ggcattgccg
     6001 ccgcattgca ggcaatcgcc gattcgggct tggacgatgt ggaaaacctc gacaaagacc
     6061 gcatcggcgt gaacatcggt tccggcatcg gcggactgcc cagcatcgag gtcaccggca
     6121 aagccgtaat cgaaggcggc gcgcgcaaaa tcaacccttt ctttatcccc ggttcgctga
     6181 tcaacctgat ttccggacac gttaccatcc tcaaaggcta ccgcggcccg agctacggga
     6241 tggtttccgc ctgtaccacc ggcgcgcacg ccatcggcaa ttccgcccga ctgatcaaat
     6301 acggcgacgc ggacataatg gttgccggcg gcgcggaagg cgcaatcagc actttgggcg
     6361 tgggcggttt tgccgcgatg aaagccctct ccacccgcaa cgacgacccc gccaccgctt
     6421 cccgtccgtg ggacaaaggc cgcgacggct tcgttatcgg cgaaggcgcg ggcatattgg
     6481 tgttggaaga attggaacac gccaaaaaac gcggcgcgaa aatctacgcc gaaatcgtcg
     6541 gcttcggcat gagttccgat gcttaccata tcaccgcgcc gaacgaagaa ggccccgccc
     6601 ttgccgttac ccgcgcgctg aaagatgccg gcatcaatcc cgaagacgtg gattacgtca
     6661 acgcgcacgg cacgtccacc cccttgggcg atgccaacga aaccaaagcc ctcaaacgcg
     6721 cattcggcga acacgcctac aaaaccgtcg tcagctcgac caaatccatg accggccacc
     6781 tgctcggcgc ggcgggcggc gtggaggccg tgtacagcat tttggcgata cacgacggca
     6841 aaatcccgcc gaccatcaac atttttgaac aagacgttga agccggctgc gatttggact
     6901 actgcgccaa cgaagcgcgc gacgcggaaa tcgacgttgc catttccaac tccttcggct
     6961 tcggcggcac caacggcacg ctggtgttca aacgcttcaa aggctgattc cgcaaagccg
     7021 ccgccgacat cgaaatgccg tctgaaaccg tttcagacgg catttttata gtgattaaca
     7081 aaaatcagga caaggcggcg agccgcagac agtacaaata gtacggaacc gattcacttg
     7141 gtgcttcagc accttagaga atcgttctct ttgagctaag gcggggcaac gccgtaccgg
     7201 tttttgttaa tccactataa tacgcaggca aacccaagcc ttgctgggtt acaggatgaa
     7261 accctgccgt agcagaccgc atttccaatc catattcaag gtgtccgcct tatgaaaatc
     7321 gtttttatca caacagtcgc atccagcatt tacggtttcc gcgcccccgt cattaaaaaa
     7381 ttaatcggca aaaaccatca ggtgtatgcc tttgtatcgg agttttccga taatgaattg
     7441 gatattatca gggaaatggg ggttacaccc gttacctacc gttcaaaccg cagcgggctg
     7501 aacccgtttt ccgatataaa atccaccttc ctcatcttta aagaactcaa aaaaatatcg
     7561 ccggatttgg ttttccctta tttcgcaaaa cccgtgattt tcggcacttt tgccgcaaaa
     7621 ctggcaggcg tgcccagaat cgtcgggatg ctggaaggtt tgggattcgc atttaccccg
     7681 cagccggaag gcataccgtt aaaaacaaaa atcataaagg ggattttgat tgccttgtac
     7741 cacattgccc tgccgatatt ggaaagcctg attgtattaa accccgacga caaagacgaa
     7801 ctgacggaca aatacggcat caaaataaaa aacatccata ttttgggcgg aatcggtctg
     7861 gatttgcggc aatatcctta ttccgaggcg gatattcccg atgaaaaaga acccgtaaaa
     7921 ttcctcttta tcggcagatt tctgaaagaa aaggggattg atgattttat tcgggcggcg
     7981 gaacaggtta aggacaaata ccccgatacg gtttttaccg ctttgggcgc aatcgacaag
     8041 tcacgcgggg ggtggggggg gggatttgga acgccttgcc gcccgcgata ttatccgttt
     8101 ccccggtttt gtgaacaatg tttccgaagt gataaaagaa catcatatat tcgtattgcc
     8161 gtcttattat agggaaggcg ttccccgaag cactcaggag gcaatggccg tcggcagggc
     8221 agtgattacg acggatgtcc ccggatgcag ggaaacggtc gccgacaagg tcaacggctt
     8281 cctgatcgag ccttggaatc cccgcatctt ggccgaaaaa atgatttatt ttatcgaaaa
     8341 cagggaagcc gtccgcctga tggggaatgc aagttatgcg attgccaaag ataaattcga
     8401 tgccgaaaaa gtcgatttga aattgctcga tattttgaag gcgtaaacaa ggctgcccgc
     8461 ttttgagttt cggtcatttc tgataaaccc ccgtcattcc cgccacaccc ccgtcattcc
     8521 ctcgaaagcg ggaattcagg ttcgtttagt ttcggtcatt tccgataaat tcttgcaact
     8581 ttgcgtttct agattcccac tttcgtggga atgacggcgg agg
//
LOCUS       DAWFIE010000078         8522 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWFIE010000078 DAWFIE010000000
VERSION     DAWFIE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.078"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(94..990)
                     /gene="argB"
                     /locus_tag="WGQ71_001706"
     CDS             complement(94..990)
                     /gene="argB"
                     /locus_tag="WGQ71_001706"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ0707675.1"
                     /translation="MAFENIISAADKARILAEALPYIRRFSGSVVVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGKFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1159..2289
                     /locus_tag="WGQ71_001707"
     CDS             1159..2289
                     /locus_tag="WGQ71_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ0707676.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTVEKSPEVFFVRERMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANVTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNVIGDLR"
     gene            2360..3211
                     /locus_tag="WGQ71_001708"
     CDS             2360..3211
                     /locus_tag="WGQ71_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ0707677.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVIFGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3294..3956)
                     /locus_tag="WGQ71_001709"
     CDS             complement(3294..3956)
                     /locus_tag="WGQ71_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ0707678.1"
                     /translation="MDSQKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEENGAIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(4051..5844)
                     /locus_tag="WGQ71_001710"
     CDS             complement(4051..5844)
                     /locus_tag="WGQ71_001710"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ0707679.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6089..7198
                     /gene="proB"
                     /locus_tag="WGQ71_001711"
     CDS             6089..7198
                     /gene="proB"
                     /locus_tag="WGQ71_001711"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ0707680.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7211..8473
                     /locus_tag="WGQ71_001712"
     CDS             7211..8473
                     /locus_tag="WGQ71_001712"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ0707681.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALFAMADSLEAATADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIETHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1903 a         2412 c         2240 g         1967 t
ORIGIN      
        1 tcaggaatga cggtttggaa attgcccgaa accccaaaaa cagaaaccgg acaaacgggt
       61 tttccgccaa agccggcatt ttccgacttt gcttcaggca tcttccccac cgcccaaaat
      121 catcgaaccg ataccggcat cggtaaagat ttccagcaaa agcgcgttgg gcaccctgcc
      181 gtcgatgatg tgcgtggctt tcacgccgtt gacggcggct tcgaccgcag aagcgatttt
      241 cggcagcata ccgccataca gcgtgccgtc ggcaatcagt tcatcaatcc gtttcggcgt
      301 gagtttggtc agcagattgc ccgttttgtc catcacaccg gcgatattcg tcatcatcaa
      361 gagtttttcg gcgttcaatt cttccgccaa tttgcctgct accaaatcgg cgttgatgtt
      421 gaacgcttcg cctttttcac ctacgccgac gggggcgaca acgggaatgc agccgcgttc
      481 tatcagccct ttaaccaaac ctgtatcgat gctttccacc gtaccgacct gtccgatgtc
      541 tacgccattc tgttcgggcg tatcgatcaa aagtttcttc gccttaatga aatggtcgtc
      601 gcgtccgctc acgccgaccg cgtgcccgcc atatgtgtta atcatcgaca cgatttcttt
      661 attgacgtgc ccgcccaaca ccatttcgac aatatccatc gcctctttgt cggtaacgcg
      721 cattccttgg acaaacttac cctttttgcc gactttttca agcatcgcat tgatctgcgg
      781 cccgccgcca tgaacgatga cgggatgaat gccgaccagc ttcagcagca cgacatctcg
      841 ggcaaaccct tctttcaagg caggttcggt catggcgttg ccgccgtact tgatgacgac
      901 gaccgaaccg gaaaaccggc ggatgtaagg cagtgcttcg gcaaggatac gcgccttgtc
      961 ggcggcggaa ataatgtttt caaacgccat aacggctccc tattgttgac aatttcggct
     1021 tatgttaacg ccaaacgccg tctgaaacaa tcttcagacg gcatccggca caccttcggg
     1081 caggttgaag ccttgcgata cgggcggtaa aatcgcggtt tatccggttc gagaaaccgg
     1141 cgacagcaag gattccccat gattaaaatc agcacccgct tcgatgccgg ctcggtcgtt
     1201 gtcaaagacc tgaccgatcc ttccaacatc cgcctcgccc tgcgtcccga caacgcctcc
     1261 gattttgcac aatggttcta cttccggctg caaggcgcgg catatcaaaa ctgcatcatg
     1321 cattttgaaa acgcggcaga agccgcctac ccgaaaggct gggaaggcta tcaggcgtgt
     1381 gcctcatacg accgccgcaa ctggttccgc gtaccgactt cctacgaaaa cggcgtactg
     1441 accgtcaatc atacgccgct gtccaacagc gtgtattacg cctatttcga accttactcc
     1501 gaagaacagc atttaaacct cctcggcgac gcgcaaggca gcggcctgtg ccgcatcgac
     1561 gacttgggca gcaccgtgca agggcgcgac atcaatctgc tgaccatagg caaccaggtc
     1621 gaaagcgatt tgaaaatctg gattaccgcc cgccagcatc cgggcgaaac catggcggaa
     1681 tggtttgtcg aaggactgct cggcaggctg ctcgatccgc aagacccgac agcacgcacc
     1741 ctgctcgacc gcgccacgtt ctatatcgtc cccaatatga accccgacgg ttcggcactg
     1801 ggcaaccttc gtaccaacgc cgccggtgcg aacctcaacc gcgagtggga aaacccgact
     1861 gttgaaaaaa gccccgaagt gttcttcgtg cgtgaaagaa tgttggaaac cggcgtggat
     1921 ttgtttttgg atatccacgg cgacgaaggg ctgccctttg tctttgttgc aggaactgaa
     1981 ggcgtgccga actacaatcc acgcatttcc gcgttggaaa cacagttcaa aaccgccctt
     2041 ttgaacgcca gccccgattt ccaagatgaa tacggttacg aaaaagatgc gcccggtcag
     2101 gcaaacgtga ccttggcaac aaactgggtc ggcaaccgtt tcaactgcct tgcctatacg
     2161 ttggaaatgc cgttcaaaga caatgccaac ctgcccgacg acgacttcgg gtggaacggt
     2221 caacgttcgt tgcgcctggg cgaagccgcg ttgtccgcga tcctcaacgt catcggcgat
     2281 ttgcgctgaa acccttattg cagaaatgcc gtctgaagca tcatgccttt cagacggcat
     2341 ttgaaaacga aagcctgtta tgattatcca tccccaattc gatcccgtcc tcatcagtat
     2401 cggcccgctt gccgtccgct ggtatgccct aagctacatc ctcggattta ttctttttac
     2461 ctttctcggc agaaggcgca tcgcgcaagg cttgtccgtt tttaccaaag aatcgcttga
     2521 cgacttcctc acttggggca ttttgggcgt aatttttggt gggcgtttgg gttacgtcct
     2581 gttttacaag ttttccgact acctcgccca tccgcttgat attttcaagg tatgggaagg
     2641 cggaatgtcg ttccacggcg gctttttggg tgtagttatt gccatatggt tgttcggccg
     2701 caaacacggc atcggcttcc tcaaactgat ggacacggtc gcaccgctcg ttccgctggg
     2761 tctcgcttcg ggacgtatcg gcaacttcat caacggcgaa ctttggggac gcgttaccga
     2821 catcaacgca ttttgggcaa tgggcttccc gcaggcgcgt tacgaagatg ccgaagccgc
     2881 cgcgcacaat ccgctttggg cagaatggct gcaacaatac ggtatgctgc cacgtcatcc
     2941 ctcgcagctt tatcagtttg cacttgaagg catctgcctg ttcgccgtcg tttggctgtt
     3001 ctctaaaaaa cagcggccga ccggacaagt cgcctcgctc ttcctcggcg gctacggcat
     3061 attccgcttc attgccgaat tcgcacgcca acccgacgac tatctcgggc tgctgacctt
     3121 ggggctgtcg atggggcaat ggttgagcgt cccgatgatt gttttgggta tcgtcggctt
     3181 tgtccggttc ggcatgaaaa aacagcactg accgcctgtg aaaaataccg tctgaaacat
     3241 ggaaatccgg tttcagacgg catttttacg tttggttcat ggaaacaaac gcttcaattc
     3301 ttaccgcaac acgccttgta ttttctgccg ctgccgcacg gacagggatc gttcctgcct
     3361 gttttttcgc cttccctgcg gacggtttgc ggtttgttga tgactgcctg ccagtagcgg
     3421 tagatatccg ccaatgcata aggcaactcg gattccagtt ccgccagctc gccttctgtg
     3481 aattgcagac ggatagcgcc gttttcctct tcgtcgtaaa taccgcccaa tgccatgatg
     3541 ggataaaaca actcttcaaa cgcttcatca tcgacggctt caaaccaatc ggtcggcaca
     3601 atatccaaac cgtaaagata agcattgcac catgtgtaaa aatcgctgcc gccgtcttcg
     3661 ttttcataca gccacaaatc gggcagtttt ttatccgaca tcgcggcggt tgtttccatc
     3721 gccattgcca aaaccagccg ttcgatttcg gaacgttcgg cggcggtaaa ttgcgattcg
     3781 tcgcccaaca cttcgggcag ccagtcgagc ggtgtcaatt tgtccggccc gctcaacagc
     3841 gccgtcataa aaccttgaac ctcgtcgcaa cgcatcgtgt tgccttgttc gcttttggca
     3901 tccaacaatt cgctcaaccg ccgtttggat gcttcggtaa atttttggga atccatgatt
     3961 ttccttttca aatgggtttt gcgccctatt atcgccgcaa tgccgtctga agccttttta
     4021 tgcttcagac ggcatcgtct gttttcacgc tcaaatcgta ccgctgcctt gcgctttcgg
     4081 tttggcggca ctaaattcca gtttgctcaa ggcggaaagg taggctttgg cggtagcgac
     4141 caaaacgtcg gtatccgcgc cctgaccgtt gacgacgcgg ttgccgcgcg ccagacggac
     4201 gctggtttcg ccctggcttt ccgtaccttg cgtgacggcg ttgacggaat aaatctgcaa
     4261 agtcgcgccg ctttgcgcca cgctttcaat cgctttgaaa attgcatcaa ccggaccgga
     4321 accggttgcg gaagcgcgtt tttcttcgcc tttgatgctg aacacgatgt cggcgcgcgg
     4381 ctcttcgccg gtttcggtgc tgattttttg ggagatgaat ttgtagctct cggcattcat
     4441 gctgcccatt tcgtcggata ccagtgcgtg caggtcttca tcgaagattt cgcgtttttt
     4501 gtcggcgagt tctttgaagc gggcaaacgc ggcgttcagt gcctcttcgc tttccaactc
     4561 gatgcccaaa tccgccagct tggttttgaa ggcgttgcgg ccggacaatt tgcccaagct
     4621 caaacggttt gttgcccagc cgaccgattc ggcggacata atctcgtaag tttcgcggtg
     4681 tttcagcacc ccatcctgat ggatgcccga ttcatgcgaa aaggcattgg caccgacaat
     4741 ggctttgttg ggctgcacgg gatagcccgt aatggtggac accagtttgg acgaaggcac
     4801 gatttgcgtg gtatcgatgc cggtttccaa gccgaacaag tcgtggcgca ctttcaacgc
     4861 catcacgatt tcttcaaccg aagcattgcc tgcacgttcg cccaagccgt tgacagtaca
     4921 ttccacctga cgcgcgccgc cttttaatgc ggcaagcgaa ttggcaaccg ccaagcccaa
     4981 atcgttgtgg cagtgtgccg accaaacgac tttgccgccg ttgggcgttt tggcaatcag
     5041 ttcgcggaaa aattcttcgg ttttgtacgg gatggaatag ccgacggtat cgggaatatt
     5101 gatggtggtc gcgcccgctt caatcaccgc gccgcagatt tcggcaagga aatcgatttc
     5161 cgaacgcaac gcgtcttcgc aggaaaattc cacatcgtcg gtgtattcac gagcgatttt
     5221 caccgctttg accgccgcct caatcacctg cttcggcttc atcttcaatt tgtactccat
     5281 atggatgggg ctggtggcga tgaaggtgtg gatgcgtttt ttcggcgcgg gcgcaacggc
     5341 ctcacccgcc tgacggatgt cccgctcgat ggcgcgggac aatgaacaga ccgttgattt
     5401 ggtaatggtt ttcgcaatcg cattgaccgc ctcgaaatcg cccgggctgg cagcggcaaa
     5461 acccgcttcg atgatgtcca cacccaattt ttccagctgg cgggcgacgc ggattttttc
     5521 ctctttggtc atagcggcgc cgggcgactg ctcgccgtcg cgcagggtgg tgtcgaaaat
     5581 gataacgcgg ttggcctgtg tcatttctgt tctctccaga gattcgtttt tttgtaaaaa
     5641 agcattcaaa tccagcggcc tgccttgcgc ttccgccaac gcggtcagct tttgcagttg
     5701 cgccagaggc agcgagccgc gcgtgcgcca tttatagatg gcggcggtcg tcgcggcatt
     5761 ttcgggatcg tgctgtttca acgcttcggc aagcgcgttc acgccgccga aataagcaac
     5821 caagcggtct atatctaatt gcatggtagt ccttgtctaa aataaatcag cctacagcag
     5881 aaatatggac aggattatac gcattttgga caaaacggca atataaattt gaaaaatctc
     5941 aataagccgc catagatttt attaatttta tacattttgt ccattttatt gcagacgcgg
     6001 cgtgattgtt gacaattgcg gcagaacagg gcaaattcgg cggcacaagg tggcaacgag
     6061 acaggacaaa ccaacaagga cggaaacaat gaaatacaaa agaatcgtat ttaaagtcgg
     6121 cacatcttcg attacccatt cggacggcag tctctcgcgc ggcaaaatcc aaaccatcac
     6181 ccgccagctt gccgcattgc atcatgcggg acacgagctg gtcttggtgt cttccggcgc
     6241 ggttgcggca gggttcggtg cgctgggttt caaaaaacgt ccggtcaaaa tcgccgacaa
     6301 acaggcttcc gccgccgtcg ggcaggggct gctgatggaa gaatatacgg caaacctgtc
     6361 ttcagacggc atcgtgtccg cgcaaatcct gctcagccgc gccgactttg ccgacaaacg
     6421 ccgctaccaa aatgccggcg gcgcactttc cgtgctgctg caacgccgcg ccgtccccat
     6481 catcaatgaa aacgatacgg tttcggttga ggaattgaaa atcggcgaca acgacacatt
     6541 gagtgcgcaa gtggcggcga tgatacaggc agacctcttg gtgctgctga ccgacataga
     6601 cggtctttac acgggcaacc cgaacagcaa tcccgatgcc gtacggctgg acaaaatcga
     6661 acacatcaac catgaaatca tcgaaatggc gggcggatcg ggttcggcaa acggcacggg
     6721 cggtatgctg accaaaatca aagcggcaac catcgccgcc gaatccggcg taccggtgta
     6781 tatctgttcc tcgctcaaac ccgatgcact tgccgaagct gccgaacatc aggcggacgg
     6841 ctcgtttttc gtcccccgtg ccaaaggttt gcggacgcag aagcaatggc tggcgttcta
     6901 ttccgaaagc cggggcagcg tttatgtgga cgaaggtgcg gaacacgctt tgtccgaaca
     6961 ggggaaaagc ctgctgatgt cgggcattgc cggaatcgaa gggcattttt cccgtatgga
     7021 caccgtaacc gtgtacagca aggcaaccaa acagcccctg ggcaaagggc gcgtcctgtt
     7081 cggttctgcc gccgccgaag acctgctcaa atcgcgtaag gcgaaaggcg tgttcatcca
     7141 tcgggacgac tggatttcca tcacgcccga aatacgcctg cttctgaccg aattttagaa
     7201 aaggaaaccc atgtcaaaca cacaaaaaca gcttgccctt gccaaagcgg caaaaaaatc
     7261 cgtcaacacg gcggatacgg aagaaaaaaa ccgcgcgctg tttgctatgg cggacagctt
     7321 ggaagcggcg acggcagata ttttggcggc aaaccgtcaa gacttggaag ccgcggcagg
     7381 caacattccc gaaagcatga ccgaccgcct tttgttggac ggcaaacgca tttgcgcgat
     7441 ggcggacggc atcagggcgg ttgccgcgct gcccgacccc gtgggcgaaa tactggaaac
     7501 ctcgactttg cccaacggct tggaaatcgt caaaaagcgc gtggcgatgg gcgttatcgg
     7561 cattatttac gaaagccgcc cgaacgttac ttccgatgcg gcggctttgg cactgaaaag
     7621 cggcagcgcg gtcgtactcc gcagcggcaa agatgcattc caatccgcac gcgccatcgt
     7681 tgccgccctg aaaacggggt tggcacaaac ccgcatcgac cccgacgcgc tccagttgat
     7741 tgaagacaca gggcgtgagg gcagttacga aatgatgagg gcgaaagatt atctagacct
     7801 gctgattccg cgcggcgggg cggggctgat acgggcggtg gttgaaaatg ccgtcgtgcc
     7861 ggtcattgaa acgggaacgg gcattgtcca catttatatc gacaaggacg ctgattggga
     7921 taaggcactc cgtatcgtgt acaacgccaa aaccagccgt ccgtccgtgt gcaactcgat
     7981 ggaagtgctg ctggtacatg aagacattgc tgccgacttc ctgcccaagc tcgaacggct
     8041 gttggttcgc gaccgtatag aagccggact gccgcccgtc cgcttccgtt tggatccgca
     8101 ggcggcgcgg catatcggcg gcgaagcggc gggtgcagac gatttcgata ccgagttttt
     8161 agactacatc ctcgccgtga aaaccgtcgc ttcggtcgaa gaggcggtcg ggcacatcga
     8221 aacccacggc acgcaccatt cagacggcat cgttaccgaa aaccgccacg ctgcggacta
     8281 tttcacgacc catatcgatt ctgccgccgt gtatgtcaac gcgtccacgc gctttaccga
     8341 cggcggcgaa ttcggcttgg gttgcgaaat gggcatctcc acgcaaaaac tccacgcccg
     8401 gggtccgatg ggtttgaaag agctgacgag ttacaaatac atcgtacaag gcacgggaca
     8461 ggttagggaa taatccgaat tcaaacgtct gatatagtgg attaacttta aaccgatacg
     8521 gc
//
LOCUS       DAWFIE010000079         8404 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWFIE010000079 DAWFIE010000000
VERSION     DAWFIE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8404
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.079"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            366..2447
                     /locus_tag="WGQ71_001713"
                     /pseudo
     CDS             366..2447
                     /locus_tag="WGQ71_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2689..4155
                     /locus_tag="WGQ71_001714"
     CDS             2689..4155
                     /locus_tag="WGQ71_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0707682.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETQREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQTDGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFDNRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4356..5324)
                     /locus_tag="WGQ71_001715"
     CDS             complement(4356..5324)
                     /locus_tag="WGQ71_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707683.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSPYRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSEFYADTPWHFTPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGKDSL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYASVLQQWEEHWFAP
                     IYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            5401..6693
                     /locus_tag="WGQ71_001716"
     CDS             5401..6693
                     /locus_tag="WGQ71_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ0707684.1"
                     /translation="MAVFPFSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAEQKQKADALLARLRGIPVTVSQSD"
     gene            complement(7042..7728)
                     /locus_tag="WGQ71_001717"
     CDS             complement(7042..7728)
                     /locus_tag="WGQ71_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ0707685.1"
                     /translation="MYAFTDAQQQKALFRLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYIFSVLFHDGGWTGLGALS
                     QFNTFVGRIALASFAAYALGQILDIFVFDKLRRLKAWWVAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(7804..8277)
                     /gene="queF"
                     /locus_tag="WGQ71_001718"
     CDS             complement(7804..8277)
                     /gene="queF"
                     /locus_tag="WGQ71_001718"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ0707686.1"
                     /translation="MSRNTEELQGISLLGNQKTQYPIGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDSQ"
     regulatory      complement(8281..8325)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         2006 a         2069 c         2508 g         1821 t
ORIGIN      
        1 ccgaaaatgc acatcatcca aaatcccgtt attcccacaa aacagaaaat caaaaacagt
       61 aacctgaaat cccgtcattc ccgcgcaggc gggaatccga cttgtccggt ttcggttctt
      121 tttctcgttt caggtgattt ctaaaccgtc attcccgcgc aggcgggaat ccagaccttt
      181 aaatcccgga catccccgat aaattcttgc cgcattaaaa ttctagattc ccactttcgc
      241 gggaatgacg gcggagaggt tttttgtttt tcctgatttt tcattgcgat gtagtgtaat
      301 gtagtatata atcattataa ttttaacact tgacaaagga aaatttctca tgacactgaa
      361 agtaagcaag taagcaagta agcaagcaag caagcaagca agcaagcggt cgggttaatc
      421 tattaacatt atctgtttta tcgctgtttt gcacgccata tgtttgaggt tcggatgcgt
      481 acgatcccgt caaagaagcc gagattaaaa acaaatttat tttagaagcg gcggaagaca
      541 gaagttccca cgtttggcgc ggcccgtgca gtgtgtcttt tgattgcttc ggtgctttca
      601 gttatcagcc cggttcggat actcgttcta caaatattgg cgatgctgcc gatttttcat
      661 ttttagacaa gccggcaaac ggtgtttccc attatttttc cagcggcaaa accgatcaaa
      721 attcatccga atacgggtat gacgaaatca atatcacggg taaaaactac aatagcggca
      781 tcctcgccgt cgataatatg cccgttgtta agaaatatat tacagatact tacggggata
      841 atttaaagga tgcggttaag aagcaattga aggctttgta cgacacgaaa cccgaagatt
      901 gggaaaaaaa taaaaaaata gttgaggaag tatatagaaa acagcttgta ggaacatttg
      961 atatacttaa acagaaaaga cccaatataa ttaatcaatt gatagaagac gccgtactta
     1021 ctccttatac accacagact aatctcgaca acatcttcaa taaaacactg cacgtcaaaa
     1081 tcgaaaacaa atcccacgtc gccggacagg tgttggaact gaccaagatg acgctgaaag
     1141 attccctttg ggaaccgcgc cgccattccg acatccatat gctggaaact tccgataatg
     1201 cccgcatccg cctgaacacg aaagatgaaa aactgaccgt ccataaagcg tatcagggcg
     1261 gtgcggattt cctgttcggc tacgacgtgc gggagtcgga cgaacccgcc ctgacctttg
     1321 aagacaaagt cagcggacaa tccggcgtgg ttttggaacg ccggccggaa aatctgaaaa
     1381 cgctcgacgg gcgcaaactg attgcggcaa aaacggcgga ttccggttcg tttgcgttta
     1441 aacaaaatta ccggcaggga ctgtacgaat tattgctcaa gcaatgcgaa ggcggatttt
     1501 gcttgggcgt gcagcgtttg gctatccctg aggcggaagc ggttttatat gcccaacagg
     1561 cttatgcggc aaatactttg tttgggctgc gtgccgccga caggggcggc gacgtgtatg
     1621 ccgccgatcc gtcccgtcaa aaattgtggc tgcgcttcat cggcggtcgg tcgcatcaaa
     1681 atatacgggg cggcgcggct gcggacgggc ggcgcaaagg cgtgcaaatc ggcggcgagg
     1741 tgtttgtacg gcaaaatgaa ggcagccgac tggcaatcgg cgtgatgggc ggcagggccg
     1801 gccagcacgc atcagtcaac ggcaaaggcg gtgcggcagg cagttatttg catggttatg
     1861 gcgggggtgt ttatgctgcg tggcatcagt tgcgcgataa acaaacgggt gcgtatttgg
     1921 acggctggtt gcaataccaa cgtttcaaac atcgcatcaa tgatgaaaac cgtgcggaac
     1981 gctacaaaac caaaggttgg acggcttctg tcgaaggcgg ctacaacgcg cttgtggcgg
     2041 aaggcgttgt cggaaaaggc aataatgtgc ggttttacct gcaaccgcag gctcagttta
     2101 cctacttggg cgtaaacggc ggctttaccg acagcgaggg gacggcggtc ggactgctcg
     2161 gcagcggtca gtggcaaagc cgcgctggca ttcgggcaaa aacccgtttt gctttgcgta
     2221 acggtgtcaa tcttcagcct tttgccgctt ttaatgtttt gcacaggtca aaatctttcg
     2281 gcgtggaaat ggacggcgaa aaacagacgc tggcaggcag gacggcactc gaagggcggt
     2341 tcggcattga agccggttgg aaaggccata tgtccgcacg cattggatac ggcaaaagga
     2401 cggacggcga caaagaagcc gcattgtcgc tcaaatggct gttttgatgc gtcgggaaat
     2461 gttttgacgt acaggcggca caccggcacg gcaccgcgcg ccgccccgca aaccaatccg
     2521 aaccctgccg ccccgaaggg cggggcatca tgatgaaacc ggcggaaaac cgccggtttt
     2581 ttgccgccgt ttgaaacccg attctagctt cagacggcat tgtcgcggca tcgggcggca
     2641 gggtttggaa cagcggcata aaaaactgat acaatccgcc gattgataat ggttattttt
     2701 tatttttgtg ggaagacatt tatgcctgca cgaaacagat ggatgctgct gctgccttta
     2761 ttggcaagcg cggcatatgc cgaagaaaca cagcgcgaac cggatttgag aagccgtccc
     2821 gagttcaggc ttcatgaagc ggaggtcaaa ccaatcgaca gggagaaggt accggggcag
     2881 gtgcgggaaa aaggaaaagt tttgcagatt gacggcgaaa ccctgctgaa aaatcccgaa
     2941 ttgctgtccc gcgcgatgta ttccgcagtg gtctcaaaca atattgccgg tatccgcgtt
     3001 attttgccga tttacctaca acaggcgcag caggataaga tgttggcact ttatgcacaa
     3061 gggattttgg cgcagacaga cggtagggtg aaagaagcga tttcccatta ccgggaattg
     3121 attgccgccc aacccgacgc gcccgccgtc cgtatgcgtt tggcggcggc attgtttgac
     3181 aacaggcaga acgaggcggc ggcagaccag ttcgaccgcc tgaaggcgga aaacctgccg
     3241 ccgcagctga tggagcaggt cgagctgtac cgcaaggcat tgcgcgaacg cgatgcgtgg
     3301 aaggtaaatg gcggcttcag cgtcacccgc gaacacaata tcaaccaagc cccgaaacgg
     3361 cagcagtacg gcaaatggac tttcccgaaa caggtggacg gcacggcggt caattaccgg
     3421 ctcggcgcgg agaaaaaatg gtcgctgaaa aacggctggt acacgacggc gggcggcgac
     3481 gtgtccggca gggtttatcc ggggaataag aaattcaacg atatgacggc aggcgtttcc
     3541 ggcggcatcg gttttgccga ccggcgcaaa gatgccgggc tggcagtgtt ccacgaacgc
     3601 cgcacctacg gcaacgacgc ttattcttac accaacggcg cacgccttta tttcaaccgt
     3661 tggcaaaccc cgaaatggca aacgttgtct tcggcggagt gggggcgttt gaagaatacg
     3721 cgccgggcgc gttccgacaa tacccatttg caaatttcca attcgctggt gttttaccgg
     3781 aatgcgcgcc aatattggat gggcggtttg gatttttacc gcgagcgcaa ccccgccgac
     3841 cggggcgaca atttcaaccg ttacggcctg cgctttgcct gggggcagga atggggcggc
     3901 agcggcctgt cttcgctgtt gcgcctcggc gcggcgaaac ggcattatga aaaacccggc
     3961 tttttcagcg gttttaaagg ggaaaggcgc agggataaag aattgaacac atccttgagc
     4021 ctttggcacc gggcattgca tttcaaaggc atcacgccgc gcctgacgtt gtcgcaccgc
     4081 gaaacgcgga gtaacgatgt gttcaacgaa tacgagaaaa atcgggcgtt tgtcgagttt
     4141 aataaaacgt tctgattgct gttccttttc ggaggaaacc ctgccggcgg cggtatcacg
     4201 gcgggcatcg gcggctttcg ggcggtgctt tgcgtgccgc cgcgtgtgcg gaaacgcatt
     4261 ccggtttttc cggcataacg gcgatgcgag gtaaaatgcc gtctgaaacc cgattcgggc
     4321 ttcagacggc attgtcgcgg ttgcggcggg cgggttcacc agattccgtc aaaggttttc
     4381 gcgccgcgcc aaaatttcca cctgtcggcg ggtttgaagg tcagcgtacc gccgtgttgt
     4441 ccgtccgtgg tgatgtccag ccgtttgatt ttgccggtac ggacggcttc gtagatcggt
     4501 gcgaaccagt gttcttccca ctgctgtaaa accgatgcat accgctccct gtcccctgtc
     4561 agggcggtca ggcgcaaatc gtccataaac aggatatggt gcgtgtcggg caggtgtgcc
     4621 gccgtttctt cataggcgcg gaagttgtcg ggcaatgcgc ggcggtcggg gtggaaacgg
     4681 ctccaaaccg tatcggcgaa aagcgtgccg ccttgactgc ttccgtgcag actgtcccaa
     4741 agccataagc cgttcaactc gggcagcccg cgtttcttgc ggttatggtt gacggggtgt
     4801 gccgccagcc acatttggat ttcggtttgg acgcgcagcc attccaacga gtctttcccg
     4861 tccggctgat cgtccgcgcc caataatccg cccaagtcca aaacgggctt cgcgccccag
     4921 cggtacgcgc ggggaaggga aaccagccat aattcgggca ggacgggcgt gaaatgccag
     4981 ggagtatcgg cgtaaaattc agacaaatcg cggcagaggc gttcggcttc atccgtaccg
     5041 acgttcagat attccgccgt cagtacattt gcctgatgca tccccatttt ctgccagacg
     5101 ggcgtggcga gcgcgacggc ttcagacggc atattcaggc tttgcgccgc gcgttccgcc
     5161 agtctgccgc accacaaata acgcgcgtaa aatgccgaag ccgtgcagct ttggcgatac
     5221 ggcgagccgt attgcaggat tttgttgaag gcgggcaggc agagaggtat tcggatttcg
     5281 tcttcatcca aattgagcga ggggagggcg agggtgagtt tcatcgtttg atgtctgaca
     5341 aatgcggatc gggcggatat tatagaggat tcggcgcaaa cgccgtcaaa aaggaacaat
     5401 atggctgtct tcccattttc ggcaaaacat cggaaatacg cgctgcgcgc gcttgccgtt
     5461 tcgattattt tggtgtcggc ggcatacatt gcttcgacag agaggacgga gcgcgtcaga
     5521 ccgcagcgcg tggaacaaaa actgccgccg ctgtcttggg gcggcagcgg cgttcagacg
     5581 gcatattggg tgcaggaggc ggtgcagccg ggcgactcgc tggcggacgt gctggcgcgt
     5641 tcgggtatgg cgcgggacga gattgcccga atcacggaaa aatatggcgg cgaagccgat
     5701 ttgcggcatt tgcgtgccga ccagtcggtt catgttttgg tcggcggcga cggcggcgcg
     5761 cgcgaagtgc agttttttac cgacgaagac ggcgagcgca atctggtcgc tttggaaaag
     5821 aaaggcggca tatggcggcg gtcggcttct gaggcggata tgaaggtttt gccgacgctg
     5881 cgttcggtcg tggtcaaaac gtcggcgcgc ggttcgctgg cgcgggcgga agtgcccgtc
     5941 gaaatccgcg aatccttaag cgggattttc gccggccgct tcagccttga cggtttgaag
     6001 gaaggcgatg ccgtgcgcct gatttacgac agcctgtatt tccacgggca gcaggtggcg
     6061 gcgggcgata ttttggcggc tgaagtcgtt aagggcggca caaggcatca ggcgttctat
     6121 taccgttcgg acaaggaagg cggagggggc ggcaattatt acgatgaaga cggcagggtg
     6181 ttgcaggaaa aaggcggctt caacatcgag ccgctggtct atacgcgcat ttcttcgccg
     6241 ttcggctacc gtatgcaccc catcctgcac acttggcggc tgcacacggg catcgactat
     6301 gccgcaccgc agggaacgcc ggtcagggct tccgccgacg gcgtgattac ctttaaaggc
     6361 cggaagggcg gatacggcaa cgcggtgatg atacgccacg ccaacggtgt ggaaacgctg
     6421 tacgcgcact tgagcgcgtt ttcgcaggca caaggcaatg tgcgcggcgg cgaggtcatc
     6481 ggtttcgtcg gttcgaccgg gcgttcgacc gggccgcacc tgcattacga ggcgcgcatc
     6541 aacgggcagc ccgtcaatcc tgtttcagtc gcattgccga cacccgaatt gacgcaggcg
     6601 gacaaggcgg cgtttgccga gcagaaacag aaggcggacg cgctgcttgc gcgcttgcgc
     6661 ggcataccgg ttaccgtgtc gcaatcggat tgaagtttga accggcgacg aaaacaatgc
     6721 cgtctgaaaa tctgcaaacc ggttttcaga cggcatttgc agttgattaa aataaaatta
     6781 agccaaggaa gcactgccgt cattcccgta cgggcgggaa tccggacacc acggcacgga
     6841 aacccatccg ccgtcattcc cgcgaaagcg ggaatctaga aatacaacgc ggcaggagtt
     6901 tatcggaaat gactgaaacc caacgcaccg gattcccgct ttcgcgggaa tgacgaagtg
     6961 gcgggaatcc ggatttatcc gttccggcag tgtttgcaaa taaaagaaaa cccaaccgtc
     7021 ccgattcccg gcagggctgt gttacggatt ttgcagcgag ggcgcggggc ggtcttgcgc
     7081 ctgtttggtt tgcagggttg tcagtttttt cgtcagcaga ttcagaatca cgccgtaggc
     7141 gggcaggaaa aacagaccgc agacggtaag tttgaacagg taatcgacaa aagcgatgcc
     7201 ctgccagttt gccgccataa atccatcgct gcttgcgtag aaggcaacgg cgaaaaatac
     7261 caacgtatcc aaggcgttgc cgatgacggt tgatgcagtc ggggcaaccc accacgcttt
     7321 cagacggcgt aatttgtcga atacgaaaat atcgaggatt tgtccgagcg cgtaggcggc
     7381 aaaacttgcc agcgcgatgc gtccgacaaa ggtgttgaat tgggacagcg cgcccaagcc
     7441 cgtccaaccg ccgtcgtgga acaaaacgga aaagatatag gaaagcaaaa gggcggggaa
     7501 catcacccaa aagataatcc gccgtgccaa gtgcgaaccg aaaatgcgga cggtcaggtc
     7561 ggtggcaagg aagatgaagg gaaaggaaaa tgcgccccaa gtggtgtgga tgccgaaaat
     7621 ttggaagggg aactgcacca gatagttgct ggcggcgatg atgaggatat gaaaaagcac
     7681 cagccggaag agtgccttct gttgctgtgc gtcggtaaat gcgtacataa aaaaaccttt
     7741 cggaaaggcg ttcagacggc ataccgtatc gaaagaatgc cgtctgaaag tgggaaggac
     7801 ggattattgg ctgtcgtgtt cgaacaagcg tttacgcgcc aatgcttcaa actcggtgcc
     7861 tgctttgccg tagttggcaa acggatgaat ggcgatgccg ccgcgcggtg tgaactcgcc
     7921 gaatacttcg atgtatttcg gatccatcag ggcaatgagg tctttcatga tgatgttgac
     7981 acagtcttca tgaaaatcgc cgtggttgcg gaagctgaag aggtagagtt tcagggattt
     8041 gctttccacc attttgatgt gcggaatata gcggatgtag atggtggcga agtcgggctg
     8101 cccggtcatg gggcagaggc tggtgaactc tgggcagacg aatttgacga aatagtcgtt
     8161 gtcgggatgt ttgttgtcga atgcttcgag aatttcaggc gcgtagccga tcggatattg
     8221 ggttttttga ttacccaaaa gcgagatgcc ttgcagctct tcggtattgc gggacatagg
     8281 gtttccttag ttttttagtg tgggaggttt tcgaaccacg gggcggcgat tgtaatataa
     8341 gcggcggtat ctgtgtagtt ttcttcagac ggcatggttt ggacggcggc gttttccgtg
     8401 tcat
//
LOCUS       DAWFIE010000080         8322 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWFIE010000080 DAWFIE010000000
VERSION     DAWFIE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.080"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            69..2183
                     /locus_tag="WGQ71_001719"
     CDS             69..2183
                     /locus_tag="WGQ71_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ0707687.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPHLSTLLPEF
                     HIYKDGKGAEAVVGWGLRPTTHKARAFATEHQLPFIALEDGFLRSLGLGVSGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQTMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADIWVKTHPD
                     VLCGKKQGYLTQLAQQHRVHLLAEDINPISLLQNVDKVYCVTSQMGFEALLCGKPLVT
                     FGLPWYAGWGVSDDRHPEINRLVQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLAGVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMGI
                     YFNAEAPSRLEHILQNQNFDDQDFQTALKLQKMLTENHISKYNVGNSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYRNLDLLRTVRERNPDAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQADILTCLQYADEVHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPILRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGYFAKKLGKIKQLCRSFK"
     gene            2320..3579
                     /locus_tag="WGQ71_001720"
     CDS             2320..3579
                     /locus_tag="WGQ71_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HEZ0707688.1"
                     /translation="MKQTVLKNNLQNLLESAENILMLQGPVGDFFLRLADWLTENGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYIVQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     EAPTPVRGGFMPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAHYDIPGMTDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            3946..5352
                     /locus_tag="WGQ71_001721"
     CDS             3946..5352
                     /locus_tag="WGQ71_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0707689.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYTEAAFVLG
                     DHLLQNGHPEQALPWLKAAAAQRHPKALFTLLQQREHNGTPTGQLLNEYAWLGEQGHL
                     EAQLILMRYHTQRNDPQSLYWAELAAAQYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAANKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGIGCHVPTACYWLQAAISNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            5570..6784
                     /gene="gltS"
                     /locus_tag="WGQ71_001722"
     CDS             5570..6784
                     /gene="gltS"
                     /locus_tag="WGQ71_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ0707690.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            6883..7899
                     /locus_tag="WGQ71_001723"
     CDS             6883..7899
                     /locus_tag="WGQ71_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0707691.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            7910..8107
                     /locus_tag="WGQ71_001724"
     CDS             7910..8107
                     /locus_tag="WGQ71_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707692.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            8125..8274
                     /locus_tag="WGQ71_001725"
     CDS             8125..8274
                     /locus_tag="WGQ71_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707693.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTVGFLCVSYADVPTHRTIQKAS
                     PYVPS"
BASE COUNT         2132 a         2414 c         1931 g         1845 t
ORIGIN      
        1 aaagacttgg ccggcaaagt attggctgaa gcggtcacgt tttaagacta ttcaggccgt
       61 ctgaaagaat gttcctcttt tcagacggcc tacaatctat taataacaat aatcgacgaa
      121 aacgcattgt gaaaaacgcc tacatcccct ctcgcggcat ccgcaaaatc ccccatctct
      181 ccaccctatt gcccgagttt catatctaca aagacggaaa aggagcagag gctgttgtcg
      241 gctggggttt gcgcccgacg acacacaaag cgcgtgcttt tgccaccgaa caccagcttc
      301 cctttattgc tttggaagac ggctttttac gctcgctcgg actgggcgtc tccggttatc
      361 ccccctactc catcgtctat gacgacatcg gcatctacta cgacactacc cgcccttctc
      421 gtttggaaca actgattctt gccgccgata ccatgccgtc tgaaaccttg gctcaggcgc
      481 aacagacgat ggatttcatc ctgcaacacc acctgtccaa atacaaccac gcgcccgaac
      541 tttcagacga tcatccttta cgctccccat ccaagcctga aactgtcctc attatcgacc
      601 aaaccttcgg cgatatggcc atccaatatg gcggcgcaga cgcctctacg tttgaactga
      661 tgtttcagac ggccttgaat gaaaacccgc aagccgatat ctgggtaaaa acccatcccg
      721 atgttttgtg cggcaaaaaa caaggctatc tgacccaact ggcgcagcaa caccgcgtcc
      781 atcttctggc agaagacatc aacccgattt ctttgttgca aaacgtcgat aaagtgtatt
      841 gcgtgacctc gcaaatgggt tttgaagcct tgttgtgcgg caagcctttg gtaacgtttg
      901 gtttgccgtg gtatgccgga tggggtgtaa gcgacgaccg ccatcctgaa atcaaccgcc
      961 ttgttcaaac ccaacgccgc gccacccgca acttgctgca gctcttcgcc gcagcctatc
     1021 tgcaatacag ccgctacctc aatcccaata ccggcgaagc aggcagcctc tttgatgtca
     1081 tcgactatct ggcgacggtc aaacgtaaaa acgacaaatt gcgtggcgag ttatattgcg
     1141 tcggtatgtc tttgtggaaa cgcgcggttg ccaaaccgtt ctttaacgta ccctcttgcc
     1201 gtctgaaatt tatctcttcc acccaaaaac tggccggggt caaactgtcc gacgatgcac
     1261 gcatcctggc ttggggcaac ggcaaagagg ccatcgtccg ctttgccgaa caacaccaca
     1321 tccccctgct gcgcatggaa gacggcttta tccgctcggt cggactcggc tccaacttag
     1381 tgccgccgct gtcgctcgtt accgacgata tgggtattta cttcaatgcc gaagccccat
     1441 cccgccttga acacatcctg caaaaccaaa acttcgacga tcaagacttt cagacggcct
     1501 tgaagttgca aaaaatgctg accgaaaacc atatcagcaa atacaacgtc ggcaactcag
     1561 actttaccgc cccatcaacc gacaaaaccg tgatcctcgt tcccggtcag gttgaggatg
     1621 atgcgtctat ccgctacggt tcgccccaaa tctatcgcaa tctggatctg ctccgtaccg
     1681 tacgcgaacg taatcccgat gcctatatca tctacaaacc gcatcccgat gtagtcagcg
     1741 gtaaccgcat cggccatatt tcccctgaag atgctgcacg atatgccgac caaacagccg
     1801 aacaagccga tatcctgacc tgtctccaat acgcagacga agtacatacc atgacttcgc
     1861 tgaccggttt tgaagccttg ttgcgcggca aaaaagtcag ctgctacggc ctgcctttct
     1921 atgcaggttg ggggcttacc caagatctgc tccccattct gcgccgcagc cgcaggcttg
     1981 agctttggca gctgattgca ggcacgctca tccactaccc cgactacatc caccccgaaa
     2041 cccatcaggc catcaatgcg gaaactgcag cccaaatcct gatacgacaa aaaaatatgc
     2101 aaaaaaacaa caacggatta catcgcgggt actttgccaa aaaattaggt aaaatcaaac
     2161 aactatgtcg atctttcaaa taaataccat caaagttaac aatgcgtcat aaacttgcct
     2221 ctgttgcggc atcattgcct ttgcatcgtt aattctcttg gcgtatgctt gaaagttcaa
     2281 cctaaaacta ttacataaaa aacaaaacca cattgcgaca tgaaacagac cgtcctcaaa
     2341 aataacctgc aaaacctgct tgaaagcgca gaaaatatcc tgatgcttca aggccctgtc
     2401 ggcgattttt ttctgcgcct tgccgactgg ctgactgaaa acggcaaaac cgtacataaa
     2461 ttcaacttta atgcaggcga cgactatttt tatccgccca ctcaagcgca taccgttgtt
     2521 tttaacgaca actacgatgc ctttcctgaa tttttgcaag aatacatcgt tcaacatcac
     2581 atccaggctg ttgtctgctt tggcgacaca cgcccttatc acgtcattgc aaaacgcatt
     2641 gcaaacgaaa accaagccag tttctgggcg tttgaagaag gctatttccg cccctactac
     2701 atcacattag aaaaagacgg cgtcaacgca ttttcaccgt tgccgcgccg tgccgacttt
     2761 tttcttgaac aattccccaa actggcccag caagaatatg aagcgccaac gccggtacgc
     2821 ggcgggttta tgcccatggc aaaaaacgct atccgttact atatcgagtt gttccgcaat
     2881 ccacgcaaat accccgacta catccaccac cgcgcaccca atgccggcca ttacctcaaa
     2941 ccgtggtcgc tctccatcct caagcgtttg aactactata ttgaagacat ccaaatcgcc
     3001 aaacgtgtgg aagcaggcaa atacggcaag ttttttattg ttcccttaca ggtattcaac
     3061 gacagccaag tccgtatcca ttgcgacttt cccagcgtcc gcagcttcct gctccatgtt
     3121 ttgagttcat ttgccgagca cgcgcctgcc gataccaaca tcatcatcaa gcatcatccg
     3181 atggaccgcg gttttatcga ctactggcgc gacattaaac gctttatcaa agaacacccc
     3241 gaactcaaag gccgcgtgat ttatgtccat gatgtccccc tgcccgtttt cctgcgccac
     3301 ggtctcggca tggtcaccat caacagcacc agcggcctgt ccggactgat tcacaatatg
     3361 ccagttaagg ttctcggccg tgcccattac gacattcccg gcatgactga ccaaaatacc
     3421 ttggcagaat tttggaatca tccgacaccg cctgacaaag agctgttcca tgcctaccga
     3481 atgtaccacc tcaacgtgac ccaaattaac ggcaacttct acagtcaggt gtttttcccc
     3541 aacaaaaaaa cctccaactc ttccacacca gtaatctgac ttagcgaagg aagttcaggc
     3601 cgtctgaaaa catttcagac ggcctgaaac aatcaatacc ttagctactg ccatgtaaat
     3661 aaaacacaaa aatctgcatt tatcattaac aataaattac aaaaacagta taatgaccga
     3721 gctgccatga gcgcataccg actcaacctg agccctttgt aacacacaaa atatggatat
     3781 atccctaggc aaaacaatat aacaagccaa acatcctaaa gataagccgg caaggcaata
     3841 cactctataa aactgtgccg agcaaaattt ttacaaagcc ctcaaccggt atcgccgccc
     3901 atatgccgca gcatccgtct tccactttat atccgcccgc aaaccatgac cgccgctcct
     3961 gatatcctct accggcaagc cgccgccctt ttggaacaat ccaataccgc ccaagccctg
     4021 cccctgctgc aacaggccgc agagcaggga tatacggaag ctgcttttgt attgggcgac
     4081 catctgctgc aaaacggtca tccggagcag gcacttccat ggttgaaagc cgccgcggcc
     4141 caacgccatc ccaaggcact ctttaccctg ctgcaacaac gcgaacacaa cggcaccccg
     4201 accggacagc ttcttaacga atacgcctgg ctgggtgagc aaggtcacct cgaagcccaa
     4261 ttaattctca tgcgttacca cacgcagcgc aacgatccac aatcgctcta ctgggcggag
     4321 cttgccgctg cccaatatgc cgcacctgcg tattaccatc tggcacgcca tcatcaacgc
     4381 caaggcgacg ttgaaacagc catcgaacaa tacgaaaaag cggcagcact cggcgtaact
     4441 gccgcctgct ggcaacttgg tcaaatctac ttctacggta caggtgtcag ccccaaccac
     4501 gcacaagccg aacactatct cgaaccagcc gcacaagccg gccacatcgc cgcacaaacc
     4561 ctgctggccg accttcttgc cgcccaacgc aaacccgaag ccttagaatg gtatcgtcgt
     4621 gccgccaata aggagcaagc ggaagcacaa gccaagctgg cccaatacgc cctgaccggc
     4681 gaactttccg aacgcgatcc gttccaagcg gcacgatatg ccaaagccgc tgccgagaaa
     4741 aaccatcctg aagccctgaa aatcatgggc gacctctacc gctacggtct cggtatcaaa
     4801 gccgacaacc atatcgcgca agattactac caccgtgccg ccgcgctggg ttctgccgcc
     4861 gcagcacaaa aactcatcag cgacgccgcg ctgtaccatc cgcaacaata cgaacaaatc
     4921 aaaactgccg ccctgcaaca acaacaaacc gaaaccatct accgtttggc ggaagcacaa
     4981 gcctgcgcca tcggccgtcc cgccgactac aatgccgcgc gaaaaaatta catggaagct
     5041 gccgggttcc accataaaaa cgcagcggca gccttaggcc gcatctacca ttacggcctc
     5101 ggtacggcgc aagatcctcg ggcggctgca cactggtacg ccattgctgc cgaacaaaac
     5161 cacccttccg cccaatacca cctcgcctgt ttttactatc acggacaagg tatcggctgt
     5221 catgttccga ccgcctgcta ctggctgcag gccgccatca gcaacggcca cacttcggcc
     5281 gaatcattaa tatccctatt agaacaatgg cgacgcgaag cacaccatgc catcggacaa
     5341 aaggccgtct gaaaagattt acactcgcat tttttgacaa tctttaacta ttcccctaat
     5401 atttgccagt tatttttcac ggacacgcca ttgttttcat ttctttctga aaacaccttg
     5461 tccgcgcatc aataccatga cactcggcgg ataacgccaa gcgttgaaac acactacatc
     5521 cggaacaaaa acggatgctc ggaaaaatat ttctaggagg tgaaacaaca tggaatggga
     5581 attcaacagt tattacacac tgattgccgc cacgctcgtg ttgctggttg gtaaatttct
     5641 ggttcaaaaa atcaaattct tacgagactt caatattccc gagccggtcg ctggcggttt
     5701 ggttgccgcg attatcctgt ttgcgctgca cgaggcgtac ggcgtgagct tcaaatttga
     5761 gaaaccgctg caaaatgcgt ttatgctgat ttttttcacg tccatcggct tgagcgcaga
     5821 tttttcccgt ttgaaggcgg gcggtttgcc gctggtggtt tttaccgcga ttgtgggcgg
     5881 atttatcttg gtgcaaaact ttgtcggggt cggactggct acggctttgg gtttggatcc
     5941 gctcatcggt ctgattaccg gttcggtgtc gctgacgggc ggacacggta cgtcaggtgc
     6001 gtggggacct aattttgaaa cgcaatacgg cttggtcggc gcaaccggtt tgggtattgc
     6061 atcggctact ttcgggctgg tgttcggcgg cctgatcggc gggccggttg cgcgccgcct
     6121 gatcaacaaa atgggccgca aaccggttga aaacaaaaaa caggatcagg acgacaacgc
     6181 ggacgacgtg ttcgagcagg caaaacgcac ccgcctgatt acggcggaat ctgccgttga
     6241 aacgcttgcc atgtttgccg cgtgtttggc gtttgccgag attatggacg gcttcgacaa
     6301 agaatatctg ttcgacctgc ccaaattcgt gtggtgtctg tttggcggcg tggtcatccg
     6361 caacatcctc actgccgcat tcaaggtcaa tatgttcgac cgcgccatcg atgtgttcgg
     6421 caatgcttcg ctttcgcttt tcttggcaat ggcgttgctg aatttgaaac tgtgggagct
     6481 gaccggtttg gcggggcctg taaccgtgat tcttgccgta caaaccgtgg tgatggtttt
     6541 gtacgcgact tttgttacct atgtctttat ggggcgcgac tatgatgcgg cagtattggc
     6601 tgccggccat tgcggtttcg gcttgggtgc aacgccgacg gcggtggcaa atatgcagtc
     6661 cgtcacgcat actttcggcg cgtcgcataa ggcgtttttg attgtgccta tggtcggcgc
     6721 gttcttcgtc gatttgatta atgccgcgat tctcaccggt tttgtgaatt tctttaaagg
     6781 ctgattttcc gcctttccga caaagcacct gcaaggttta ccgcctgcag gtgcttttgc
     6841 tatgatagcc gctatcggtc tgcaccgttt ggaaggaaca tcatgtatcg gaaactcatt
     6901 gcgctgccgt ttgccctgct gcttgccgct tgcggcaggg aagaaccgcc caaggcattg
     6961 gaatgcgcca accccgccgt gttgcaaggc atacgcggca atattcagga aacgctcacg
     7021 caggaagcgc gttctttcgc gcgcgaagac ggcaggcagt ttgtcgatgc cgacaaaatt
     7081 atcgccgccg cctacggttt ggcgttttct ttggaacacg cttcggaaac gcaggaaggc
     7141 gggcgcacgt tctgtatcgc cgatttgaac attaccgtgc cgtctgaaac gcttgccgat
     7201 gccaaggcaa acagccccct gttgtacggg gaaactgctt tgtcggatat tgtgcggcag
     7261 aagacgggcg gcaatgtcga gtttaaagac ggcgtattga cggcagccgt ccgcttcctg
     7321 cccgtcaaag acggtcagac ggcatttgtc gacaacacgg tcggtatggc ggcgcaaacg
     7381 ctgtctgccg cgctgctgcc ttacggcgtg aagagcatcg tgatgataga cggcaaggcg
     7441 gtgaaaaaag aagacgcggt caggattttg agcggaaaag cccgtgaaga agaaccgtcc
     7501 aaacccacgc ccgaagacat tttggaacac aatgccgccg gcggcgatgc gggcgtaccc
     7561 caagccgcag aaggcgcgcc cgaaccggaa atcctgcatc ctgacgacgg cgagcgtgcc
     7621 gataccgtta ctgtatcacg gggcgaagtg gaagaggcgc gcgtacaaaa ccagcgtgcg
     7681 gaatccgaaa ttaccaaact ttggggagga ctcgataccg acgtgcaaaa agagttggtc
     7741 ggcgaacaac gcaagtgggc gcaggaaaaa atcagcaact gccgacaagc cgccgcgcag
     7801 gcagaccggc aggaatacgc cgaatacctc aagctgcaat gcgacacgcg gatgacgcgc
     7861 gaacggatac agtatcttcg cggctattcc atcgattagg ggcaaaccga tgactaccgt
     7921 ccccaaaagc aggattcccg tcaaaccgct gcccgaaaaa accacagacg aagccaaagt
     7981 cgaaaaatgg cggcagctcg gtgcggaaca cggtttgtcg ggcgaatggg cagttgccgc
     8041 cagattgggc gagaacggtt ttaccgaaga acagatggaa aatatcgcca acctgttcgg
     8101 cagataaagg gaaaattgac ggaaatgccg tctgaaaccc tgttatcggt ttcagacggc
     8161 attttgacta atacggtacg caggcgcaaa acagtcggct ttttatgtgt ttcctatgct
     8221 gatgttccaa cacacaggac gatacaaaaa gcgtcgccct atgtgccgtc ctgattcgga
     8281 aagggttacg ctcctcccaa atatagtgga ttaacaaaaa cc
//
LOCUS       DAWFIE010000081         8299 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWFIE010000081 DAWFIE010000000
VERSION     DAWFIE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.081"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WGQ71_001726"
     CDS             complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WGQ71_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ0707694.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALGVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TSSSGSQEIGEYREVKAADVAEMLKSANSVIITPGYGMAVAQAQYPVAEITELLHKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1659..2042)
                     /locus_tag="WGQ71_001727"
     CDS             complement(1659..2042)
                     /locus_tag="WGQ71_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ0707695.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLAHTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAAQSTINTLACLFILFRLAFIWCYIA
                     DKAAMRSLMWAGGFACTVGLFVAAA"
     gene            complement(2046..3587)
                     /locus_tag="WGQ71_001728"
     CDS             complement(2046..3587)
                     /locus_tag="WGQ71_001728"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ0707696.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWVCPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLN
                     MFKKG"
     gene            3877..4710
                     /gene="serB"
                     /locus_tag="WGQ71_001729"
     CDS             3877..4710
                     /gene="serB"
                     /locus_tag="WGQ71_001729"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ0707697.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SGADAALLGSRQIDHAVLPDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGVGVAYRAKPKAQAAADACINFGGLERVRGLF
                     R"
     gene            4774..5997
                     /locus_tag="WGQ71_001730"
     CDS             4774..5997
                     /locus_tag="WGQ71_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0707698.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGVY
                     TSFREGVAQASSMRRVVVLTLCGAVAGVGWCLLKALGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGAAAVFFQRTAQKFPFIKRDNIKIIPLAVCMFVLIGVISVWFP
                     EVLGNGKAGNQLTFSGLTGWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSGSAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6158..7738
                     /gene="purH"
                     /locus_tag="WGQ71_001731"
     CDS             6158..7738
                     /gene="purH"
                     /locus_tag="WGQ71_001731"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ0707699.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLTKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLGEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGEPQIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDVYPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
BASE COUNT         1838 a         2257 c         2358 g         1846 t
ORIGIN      
        1 taaaaatcca tcttcaacac ggagatggat tttgtttgtt tcgttgattt tgtgtcagtt
       61 tcagatgtag gtgcttattc ggacgggccg tctgaaaatg tttgccccaa tgcaaaaaaa
      121 atcactgcaa accttcataa acggggtttg cagtgatttt ttcaaatcaa acagattgaa
      181 aacctgcgcc gaattgttca gacggcatta ttttttcagt tcggacagaa tgtcatctac
      241 ggttttcttc gcatctccga aacacattac gctgttttcg ttgaagaaca gcgggttttg
      301 tacacctgcg tagccggtat tcatcgagcg tttgaagacg acgacttctt ttgccttcca
      361 tacttccaac acgggcatac ccgcaatcgg gctgttcggg tcggtttggg cggcaggatt
      421 gacggtatcg ttcgcaccga tgaccaagac cacatcggtt tcggggaaat cgtcgttgat
      481 ttcgtccatt tccaacacga tgtcgtaggg gactttggct tcggcaagca gtacgttcat
      541 atgaccgggc aggcggccgg cgacggggtg gatgccgaag cgtacttctg tgccgttttt
      601 atgcaaaagc tcggtgattt cggcaacggg gtattgcgcc tgtgcgactg ccatgccgta
      661 gcccggggtg atgatgacgc tgtttgcgct tttcagcatt tcggcaacat cggcagcttt
      721 gacttctcgg tattccccta tctcttggct gccggaagat gatgtgccgc tgtcgctgcc
      781 gaaaccgccg gcaattaccg agacaaacga gcggttcatg gctttgcaca taatgtagga
      841 cagaatcgcg ccgcttgagc caaccagcgc gccggtaacg atgagcaggt cgttggagag
      901 cataaagcct gccgctgcgg ccgcccagcc ggagtaggag ttgagcatgg acacgaccac
      961 gggcatatct gcgccgccga tggaggcaac caagtgccag ccgaatgcga gggcaatcag
     1021 ggtcataatc agcaggatga agccgctgcc gtcaatgccg acgaatacga gcagcaacac
     1081 aaacgatacg ccaagtgcca gtgcgttgag cttgtgtttg gcgggcagtt gcagcgggct
     1141 gctgctgatt ttgccgttga gcttgccgaa tgcgaccagc gagccggtaa aggttaccgc
     1201 gccgatgaaa atgcccaaat agacttcgac cagatggatg gtgtgcatat cgtgcgaaac
     1261 gttgcccggc gcgatatagc tgttgaagcc gaccaaaacc gccgctaggc cgacgaagct
     1321 gtgcagcagg gcaatcagtt cgggcatttc ggtcatttcc acctttttgg ctttgtagat
     1381 gccgattgcc gcgccgatga gcatggcgat gatgatccag cccagtccgt gggtattgtc
     1441 ggaaaaaaca gttacaaaaa gggcgaccgc cataccggcg ataccggaat agcagccctg
     1501 tttggcggtt tcctgtttgg acagccccgc cagtgagaag atgaataaaa ttgcggcaac
     1561 gatatacgcc gctgttacga gtcctgaaga catggaaatt ctccgatttt cgatgatttg
     1621 ttttcaatgc cgtccgaact gttcagacgg tatctgtttc aagcagccgc gacaaacagc
     1681 ccgacggtgc aggcaaatcc gcctgcccac atcagtgagc gcatagcggc tttgtcggcg
     1741 atatagcacc agataaaggc gaggcggaac aggatgaaca ggcaggcaag cgtgttgatg
     1801 gtcgattgcg ccgcattgcc ggttgcgtgt gccgtcaaaa cggcggcggc aaacggtgca
     1861 aaggcttcaa aaccgttttg ctgcgcggcg tgggcacggg cggctgtgcc ttgcgtgtgc
     1921 gccagaaaat cgcgcggatt gtggttgtct ttaaaccgga atccgcccgc ttttttggca
     1981 tatgccgcac aaaaaagcgg caataggcag gcaatcagga tgcaccaata ggcgaaagtc
     2041 atggcttacc ctttcttaaa catattcagc atacgccgtg taaccgcaaa gccgccgaag
     2101 atgttgatgc cggcaatcag gatggcaaca aacgacagca gcgaaacaaa gccgttgccc
     2161 tgaccgattt gcagcagcgc gccgacgacg atgatgccgg agatggcgtt ggtcacggac
     2221 atcagcggcg tatgcagcga gtggctgaca ttccaaacga cgtagtaccc gatgacgcag
     2281 gcgaggacga acacgataaa gtggttcaag aatgctgcgg gtgcgaccgc gccgacccac
     2341 agcactaata cggcggcgat gacggcaggc gcgagttttt tccacagggg aacgggtttc
     2401 ggctcgggct tggcggcagg cgcggctttt tcagacggcg tttgctgcgg ctgggcggaa
     2461 acttgaatcg gcggaggcgg gaaggtgatt tcgccgtcgc gggtaacggt catgttacgg
     2521 ataatcacgt cttcaaaatc cagcgtgatt tcgccgtctt tgttcgggct taacagcttg
     2581 gtcaggttca ccaagttggt ggcgtaaagc tgggaagact gtccggcaag gcggtttgcc
     2641 atgtcggtgt agccgatgat tttcacgccg ttgccggtta cggacaattc gcccgggcgg
     2701 gtgagttcgc agttgccgcc cgtcgccgcc gccaaatcga cgatgacgga gccggatttc
     2761 atgctttcca ccatttcttt ggtaatcagc ttgggcgcgg gtttgcccgg aatggcggcg
     2821 gtagtgatga tgatgtccac ttctttcgcc tgctcggcaa agagtttcat ctcggcggcg
     2881 ataaattcgt cgctcatcac tttggcgtag ccgtcgccgc tgccgcccga ttcttgcggg
     2941 aagtcgagtt tcaggaactt gccgcccatc gattcgattt gttccgccac ttccaagcgg
     3001 gtatcgaacg cgcgcaccac tgcgcctagt gaatttgccg taccgattgc cgccaaacct
     3061 gccacacctg caccaatcac caaaacctgc gcaggcggca ctttgccggc ggcagtaatt
     3121 tgaccggtga agaaacggcc gaaggcgttg gcggcttcaa ttacggcgcg gtagccgctg
     3181 atgtttgcca tcgaagacaa agcgtccaaa gcctgcgcgc gcgaaatgcg gggcaccata
     3241 tccatcgcca gcgcgttcac tttcttggcg cgcaaggctt cgaccaaagc ctcgttttgg
     3301 cgcggccaca ggaagctgac gatggtttga ccttcgttca aaagcggcag ttcctgttcg
     3361 gacggcgcgt tgaccttata aatcaaaggg cagacccaaa ccgccgcttt gtcggcaacg
     3421 gttgcgcctg ctgtttggta agcggcatcg tccaaacttg ccgccaaacc tgcaccgctt
     3481 tcgacaacgg tttcaaagcc cagtttgccc agcagggcga cggtggcggg cgtacaggcg
     3541 acgcgggttt cgccggataa tgactcgcgt gggataccga ttttcatctc tgaatccttt
     3601 ttcgggtagt ttatatgtat cgtgggttaa atttaaatcg gggcagggcg gagcaacgcc
     3661 gtaccggttt aaaaccgatt cacttcaaat gttaatatat tttagataat ccccttataa
     3721 cgaattttca tcaggctggc aatagttgcg gcattttccc gtgttgtccg acacatactg
     3781 ccgtttgcgt ttgaagtcat tttccgattg cagtccctcc cgccgtaaca ttctttgccg
     3841 tttcactata taatccgcat tttttgagcc gtcgttatgc cgcacgccct cgtcctccaa
     3901 tttccctccg ccgaagccct gccttccgac ttcccctccc gcctgcccga acctgattgc
     3961 gccgatgaaa agcgtatgcg ttttatcgtt gaagaagggt tttctttaag cggggcggac
     4021 gcggcgttgc ttggcagccg tcaaatcgac cacgccgtgt tgccggatat ggattttggc
     4081 gaactcggtt tgattgtcag cgatatggat tcgacgctga ttaccatcga atgcgtcgat
     4141 gaaattgcgg caggcgtggg tttaaaagac aaggtagcgg aaattaccga gcgttcgatg
     4201 cgcggagagt tggatttcga gcagtcgttg cgcagccgcg ttgcgctgtt ggcgggattg
     4261 gacgaacaga ttttggcgga cgtttatgaa aacgttttga agctctcgcc cggtgcggaa
     4321 tttttgttgg acgaatgcaa aaggcacgat gtgaaattcc tgctggtgtc cggcggcttc
     4381 acgtttttta ccgaacgtct gcaacaacgc ctcggcttcg aataccaaca cgccaatgtt
     4441 ttggaaattg aaaacggcag gttgaccggc cgtctgaaag gcagaatcat cgacgcgcag
     4501 gcaaaggcgg atttattgcg cgaataccgc agccgcctcg gattgcagcc acatcaggtg
     4561 ttggcgatgg gcgacggtgc gaacgatatt ccgatgctca aagaagcggg cgtgggcgtg
     4621 gcttaccgcg ccaaaccgaa agcgcaggca gccgccgatg cctgtatcaa tttcggcggt
     4681 ttggagcgtg tacgcggcct gttcaggtag gcggatagga aacggatgcc gtctgaaagg
     4741 tgttcagacg gcatttgaac ggcaggaacg acagtgggac gcagaaaact ttggtttgcc
     4801 ctggcagcag cgggcgttat cggcggtttg gtcggcattg tgctgacgga actgatgcac
     4861 ttcatacagc atacggcata cggttatggc gcggacggcg tgtacacttc gttccgcgaa
     4921 ggcgtggcac aggcttccag tatgcggcgc gttgtcgtgc tgacgctgtg cggcgcggtc
     4981 gcaggagtcg gctggtgctt gctgaaagcc ttgggcaagc cgcaaatcga aatcaaagcc
     5041 gccttgaagc agccgttgca ggggctgccg tttctgacga cggtttttca tgttctgctg
     5101 caaatcataa cggtcggact cggttcgccg ctcggacggg aagtcgcgcc gcgtgaaatg
     5161 actgccgcgt ttgcttttgc cggcggcagg cggttgggtt tggatgaaga cgaaatgcgc
     5221 ctcttgattg cctgtgcgtc cggcgcgggt ttggcagccg tgtataatgt gccgctcgcc
     5281 tccacacttt tcatcctcga agccatgctg ggcgtgtgga cgcagcaggc ggttgccgcc
     5341 gcgttgctga cttccgtcat tgccaccgcc gtggcgcgta tcggcttagg cgacgtacag
     5401 cagtatcatc ccgccaacct tgccgtaaat acttcattgc tttggttttc cgccgtcatc
     5461 ggcccgatat tgggcgcagc cgccgtcttt ttccagcgta ccgcccaaaa gttccccttt
     5521 atcaagcgcg acaacatcaa aattattccc ttggcagtct gcatgtttgt gctcatcggc
     5581 gtgatttccg tttggtttcc cgaagttttg ggcaacggca aagcaggcaa ccaactgact
     5641 tttagtggtt tgaccggttg gcaacacagc cttgagctga ccgccgtcaa atggctggtt
     5701 gtcttgatgg cgttggcggc aggcgcatac ggcggactga ttacgccgtc tatgatgctc
     5761 ggcagcacca tttcttttgc agccgccgcc gcgtggaaca gcgttttccc cgaaatgccg
     5821 tctggaagcg cggcagtcgt cggagcggcg gtgtttctcg gcgtgtcgct caaaatgccg
     5881 ctgaccgcca ttgtgttcgt attggaactg acctacgcgc cgctgtccct gctgctgcct
     5941 ttgtgcatcg gcatggcggg ggcattggga acatcgggaa aaatgggctt caaatgaatg
     6001 ccgacaaatg ccgtctgaaa ggttttcaga cggcatttct ttgtaaattg tcagcaatat
     6061 ctgcaaaaac ctactgccaa aaaactagaa atagcggata ataccgcccc gaaaaagttc
     6121 ccctttcccc atccaactgt cagaaaagga agccccaatg tccgtcatca aacgcgccct
     6181 gatcagcctg tccgacaagg caggcgcggt cgaatttgcc caaaccctga ccaagctcgg
     6241 tgttgaaatc ctctctaccg gcggtacagc aaagctcttg gctgatgcag gcgttcccgt
     6301 tatcgaagtt gccgactata ccggcttccc cgaaatgctc gatggccgcg tgaaaaccct
     6361 gcatccgaaa atccacggcg gcatcctcgg tcgtcgcgat ttgggcgaac acgtcgccaa
     6421 gatggaagaa cacggtatcg gcaacatcga cctcgtgtgc gtcaaccttt accccttcgc
     6481 cgccaccatc gccaaaccaa actgcacact ggaagacgcg attgaaaaca tcgacatcgg
     6541 cggcccgact atggtgcgtt ctgccgcgaa aaactggaaa cacgtcgcca tcgttaccga
     6601 taccgccgat ttccctgcca tcgccgccga actcgaagcc aacaacggcg cattgagcga
     6661 caaaacccgt ttcaacctct cacgcaaagc attcagccat accgcccaat acgacggcat
     6721 gatttccaac tacctgacct cgctttcaga cgacgtcttg agcggagagc cccaaatcgg
     6781 cgaattcccg agccagttca accaaagctg gattaaagtg caagatatgc gctacggcga
     6841 gaatccgcac caacgcgccg cgttctaccg cgatgtgtac cccgccgcag gcagcctttc
     6901 cgcctacaaa cagctccaag gtaaagaatt gtcgtacaac aacatcgccg atgccgatgc
     6961 cgcatgggaa gccgtcaaat cctttgacgc gcccgcctgc gtgattgtga agcacgccaa
     7021 tccgtgcggc gtcgccgttg cagccgatac cttgaccgcc tacaaactcg cctacgccac
     7081 cgacaccacc agcgcgttcg gcggcatcat cgccttcaac cgcgaagtgg acggcgaaac
     7141 cgtcaaacag attacagaca accagtttat ggaagtcctg atggcgccga agttcaccgc
     7201 cgaagccctc gaaatcgccg ccgccaagaa aaacgtgcgc gtgttggaag tgcctttgga
     7261 agcaggcgcg aaccgctttg aactcaaacg cgtcggcggc ggattgttgg tacaaacgcc
     7321 cgacatccac cgcatcagcc gcgccgattt gaaagtcgtc tccaaacgcc aaccgaccga
     7381 acaggaatgg aacgatttgc tgttcgtttg gaacgtcgca aaatacgtca aatccaacgc
     7441 catcgtcttc ggcaaaggcg gtcaaaccta cggcatcggc gcaggccaaa tgagccgcgt
     7501 ggacagtacc cgcatcgccg cccgcaaagc gcaggacgca ggcttggact taaacggcgc
     7561 atgtgccgcc tctgatgctt tcttcccctt ccgcgacggc gtggacgtga ttgccgagca
     7621 gggcatcaaa gccatcatcc atccggcagg ttcgatgcgc gatcaggaag tcttcgacgc
     7681 agcggacgaa cacggcatcg ccatggtcgt aaccgacgtg cgccacttcc gccattaacg
     7741 cagatgaaca aggcaatgcc gtctgaaggg ctttcagacg gcattttgcg ctattttgtg
     7801 aaggtaggga tgacggttcg ggtattcctg acagggtgga ttttcaaggt gttgtatagg
     7861 gtgtaggagg attcgtaaaa ggtgggatgc agggtgtgct tcagcccgct gcatcaaaaa
     7921 tttttggaga atcggcggga gtcggcggtt ttggtttcgg cggggacggt ggaaatgggt
     7981 aacattgacg gaatcgacgg aagcggtggg cttcagccca ccgcttgtat attggaatca
     8041 ccgtatcata gcgccacccg cgcccaccca aggcacacaa ccgttgcgta gctcagggag
     8101 cggcagggca acccatcgac acaaccggac agttgccgga caacacaacc gaatgcaagg
     8161 caggttgatg atgagtaccc gataccatta cgcaggtata gtgaattaac aaaaaccagt
     8221 acgacgttgc ctcgccttag ctcaaagaga acgattctct aaggtgctga agcaccaagt
     8281 gagtcggttc cgtactatc
//
LOCUS       DAWFIE010000082         8261 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWFIE010000082 DAWFIE010000000
VERSION     DAWFIE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.082"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            140..1081
                     /gene="miaA"
                     /locus_tag="WGQ71_001732"
     CDS             140..1081
                     /gene="miaA"
                     /locus_tag="WGQ71_001732"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ0707700.1"
                     /translation="MPIPKAFALLGPTACGKTALALKIAETLPVEIISLDSALVYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKCPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIERALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            1085..1804
                     /locus_tag="WGQ71_001733"
     CDS             1085..1804
                     /locus_tag="WGQ71_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEZ0707701.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLQSLLDDTLPQEMSQAHKARLLAELKNHPPVAAFPSQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGREIQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            1809..2297
                     /locus_tag="WGQ71_001734"
     CDS             1809..2297
                     /locus_tag="WGQ71_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ0707702.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYVAGILGVKYTGAGRLAVRGALVGSIVGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLIVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            2363..3115
                     /gene="rlmB"
                     /locus_tag="WGQ71_001735"
     CDS             2363..3115
                     /gene="rlmB"
                     /locus_tag="WGQ71_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ0707703.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFVDTTRLDAISKGARHQGVAGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMSGESDLYHCNLPDRAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKV"
     gene            complement(3177..4568)
                     /locus_tag="WGQ71_001736"
     CDS             complement(3177..4568)
                     /locus_tag="WGQ71_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ0707704.1"
                     /translation="MSGHMGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            4875..5750
                     /locus_tag="WGQ71_001737"
     CDS             4875..5750
                     /locus_tag="WGQ71_001737"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ0707705.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFFVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            5759..6955
                     /gene="bamC"
                     /locus_tag="WGQ71_001738"
     CDS             5759..6955
                     /gene="bamC"
                     /locus_tag="WGQ71_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ0707706.1"
                     /translation="MPSEAFRRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSHAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKTTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(7012..7944)
                     /gene="pip"
                     /locus_tag="WGQ71_001739"
     CDS             complement(7012..7944)
                     /gene="pip"
                     /locus_tag="WGQ71_001739"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ0707707.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIRKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT         2130 a         2675 c         1924 g         1532 t
ORIGIN      
        1 ccgtactggt ttttgttaat ccactatata aaggcatacg gtaaacacgc ccccttgccg
       61 tcggcgtgct tccgcaaacc aaaatataac gtcggcacgc tttccgctat aatccgcatt
      121 ttcgcaaccc tgaaacatca tgccgattcc caaagccttt gccctcttag gcccgaccgc
      181 ctgcggcaaa accgcgctcg ccctcaaaat tgccgaaacc ctgcctgtcg aaatcatcag
      241 cctcgattcc gcgctagtct ataccggtat ggacatcggc acggcaaaac cgtccgcctc
      301 cgaacgcgcc tttgtcccgc accaccttat cgacatcatt acgcctgttc agacatacag
      361 tgccgcccgt tttgtcgaag actgcacgcg tctgatcggc gaaatcacgg cgcgtgggaa
      421 atgtccgctg attgtcggcg gcacaatgat gtatttccgc gccctgaccc aaggtttgaa
      481 cgatttgccc gaggccgatg cctgcctgcg tgccgacttg gacgaacaaa aacagatgta
      541 cggcttggat ttcctctacc gcaccctgca aaaagtcgac cctgaaaccg catgccgtct
      601 gaaaccgaac gacagccagc gtatcgaacg cgctttggaa gtttattatc tgaccggcaa
      661 accgatgagc gaacacctcg gcagacaaag cccccatacg ctccccttcg atttgcatac
      721 cgccgccctg attcccgaaa accgcgcccg cctgcatgaa aacatcgccc tgcgtttcca
      781 cctgatgctt gaacagggct ttatcggcga agtagaaaac ctgcgccgcc gctatcccgg
      841 cctgaccgcc gactcccccg ccatccgctg cgtcggctac cgtcaggcat gggaatatct
      901 agacggaaaa accgatttcc cggcatttgt cgagaaaggc attgccgcca cccgccaact
      961 tgccaaacgc caactgactt ggttgcgcaa aacaccttta gactgcgttg ccgacccgtt
     1021 ttcagacggc acttcatgca cacgcctgat tgaggcggca aaacggtttt tcggcgcata
     1081 aaccatgctg accacaccgc cgctcgcccc caaaaccgtc gccgccctgc acaggctcgg
     1141 tatccgtacg ctcgaagaac tgcggcaaaa cggttcggtc aaagcctttc tgctgctcaa
     1201 agcatcgggt ttgaccctca cgaaaagcac actgtggcag cttcagtcgc tgctggacga
     1261 cacgctgccg caagaaatgt cccaagcgca taaagcccgc cttcttgccg aattgaaaaa
     1321 ccatccgccc gtcgccgcct tcccatcgca ggaagaaatg gaacacttca tgcgcgaagc
     1381 actgcgccaa gcagaacaat ccgccgccga cggcgaaata cccgtcgggg cagtcattgt
     1441 ttcagacggc aaaatcatcg catcggcaca caacacctgc attgccgact gcaatgtcag
     1501 ccgacacgcc gaaatcaacg ccttggcaca ggcaggcaga gaaatacaaa actaccgcct
     1561 tgacggatgc gacatatata tcaccctcga accctgcgcc atgtgcgcgt ccgcactgat
     1621 acaggcacga atcaggcgtg tgatatatgg tgcggcagaa cccaaaaccg gcgcggcagg
     1681 cagcattgtc aacctgtttg ccgacaaacg cctcaatacg cacaccgcca tacggggcgg
     1741 aatcctgcaa gaagaatgcc gcgccgtatt aagccgtttt tttcagaaca aaagaaaagg
     1801 ttgaacctat gaccgtactg accgtcatcc tcgccctcgc cctgatagcc gtcggcacgg
     1861 cgggcatcgt ttaccccgcc ctgcccggat tggcattgat gtttgccgga acatggctgc
     1921 ttgcctactc cggcggctac caaatctacg gcgcaggtgt tttgtggacg gtcggactca
     1981 tcagccttgc cggcatactg gcggactatg tggcaggcat attgggggta aaatataccg
     2041 gagcgggcag gctcgccgtt cgcggcgcat tggtcggcag catcgtcggc atatttttct
     2101 cccttcccgg actaatactc ggccccttta tcggcgcggc ggcaggcgaa ctcatcgaca
     2161 gacgcaatat gcttcaggca ggtaaagcgg gcttgggtac gctgttgggg cttatcgtcg
     2221 gtacggcgtt caaaatcggc tgcgccgtat ccatcttgtt tatcctgttg gtgaaataca
     2281 tcgcctacct gttttaaccc cttcagacgg catacggata aattgctata atgccgtctg
     2341 aaatttccga caaagcacca tcatggcaaa ccaaagaccc atctacggct tccacgccgt
     2401 caatgcccgt ttgtggcaaa accccaaatc catcgtcgaa ctctacatcc aagaaggcaa
     2461 atcagacgca cgcacgcgcg aagtgttgga aaaggcggca aacgaaaaca tccgcgtata
     2521 ttttgttgac accacccgtc ttgatgccat cagcaaaggc gcgcgccatc agggtgtggc
     2581 cggatttatc gatgcctcca aaaaccacgt ccatctcgaa gacgtattgg aaaacttaag
     2641 cgaaccgccg ctgctgctga tactcgacgg catcaccgac ccgcacaacc tcggcgcatg
     2701 cctgcgtacc gccgacgcaa tgggcgtaca cgccgtcatc gcaccgaaag acaaaagcgc
     2761 ggggctgaac gccaccgtca gcaaagtcgc ctgcggtgcg gcggaaaccg tcccctatat
     2821 caccgtaacc aacctcgccc gcaccctgcg cgaattgaaa gaatacggca tttggattat
     2881 cggcaccgat atgagcggcg agtccgacct ttaccattgc aatctgcccg acagagcggc
     2941 gtgggtaatg ggcaacgaag gcgacggtat gcgccgcctc acgcgcgaac attgcgacat
     3001 gctggtgtct atacccatgt tcggcacagt cgaaagcatg aacgtctccg tgtctgccgg
     3061 aatggtatta agtgaaacac gccgacaacg cgtattaaaa aacgaaaaag tataagcctc
     3121 aaaaacaatg ccgtctgaaa cagggattct gtttcagacg gcattcattt tcaaaatcag
     3181 tgttccaagt ggtcggtatc aaacttgacc gccgcctcgg ttttatcttc gggcaagacc
     3241 aaattcagca agactgccgt aatgccgccg gcggaaatag agttttggaa caagacgggc
     3301 aggtttttaa acacttccgg ctcaaacgcg acacccaagc ccaaaccgac cgacgttgcc
     3361 gcaattaccg cttcgcgcct gcggatgccg tgactgacca aaatccgcac gcccgcaatc
     3421 gcaatcaagc cgaacatcaa aaccatcgcg ccgcccaaca ccggactcgg aatcgtcgta
     3481 aacgcgcgtc cgacaacggg gaacagaccc aacagcacca aaatcacggc aatatatttg
     3541 cccacatggc gcgaagccac gccggtcatc tgaatcacgc cgttgttttg cgcaaacgtc
     3601 gtcagcggca gcgaacccaa agccgtcgca atcaccgaca ccaagccgtc agccaacacg
     3661 ccgccgcgca ggcgtttggt gtattcctcg ccttcaatcg gctggtcgga caccattgcc
     3721 gtcgcggtta aatcgccgac cgcctcaaac acgctcaaca agaaaatcgc gcccgccaca
     3781 ataaacgcgt gccagtcgaa agcaaaaccg tatttaaacg gtacgggcag cgtaaccagc
     3841 ggcaggtttt gcagcgcgga aaaatccacc ttgcccaaaa acagcgcgac gatatagccg
     3901 gcaatcagcc cgaccgcaat gccgctcatg cgcaacagcg ggtttttcat gcagttgaac
     3961 accaacacaa tcagcaacac cagcgaagcc agccccaagt tttccatcga gccgaacgtg
     4021 ccgtccgcct tcgcgccgaa gccgccgccg aaatcggtaa tgccgacgtg taccaaactc
     4081 aaaccaatga gcatcacgac cacgccgctg accgtcggcg taatcacttt tttcaaatac
     4141 ggcagaagcc acgccgagaa acacaccaaa aacgcgccga caaacgatac gcccaagagc
     4201 gtcgaaatca tcgcatcctt agtcaaaccg ccctctttca tccccgcgcc cagcgcaatc
     4261 atgacggtaa cgaacgagaa attcaccgac tggatggaca gcatccccga accgaccggc
     4321 ccgaagcggt tgacctgcaa ataagtgccg acacccgacg caaccatcgc catcgacacg
     4381 agatacgccg tcatctccac cggcagttcc agcgcgccgc ccacaatcag cgcgggcgta
     4441 atcatcggca cgaaaatcgc caaaagatgg gtaaccgcgc tcaacagcgc attaccgaac
     4501 ggcggcctat cctccaaacc gtacaccaaa tcaggcgcat ccgccccttt tcccatatga
     4561 ccgctcataa tgtttccttg cttcaaatgt tgaaaaaacc aaagcgcgga ttgtagcgaa
     4621 tccgccatac cgcttcaatt tcgatttcgg acggcatacg cgcccgtccg gcaaacagat
     4681 tccgaaccgt cgccgccgga atatcgttta caaaacgcaa taacctgatt tcaaacacaa
     4741 aaatacaaac ccgacgcatc cgccgtcaag ccgccccgct catcccggac ccgccggatt
     4801 tcacacctgc ggtaaagtag gctacaatcg gaagcattac cgccccctac accacaactg
     4861 ataaaaggtt ctgtatgtta caaggtagcc tggttgccct gattaccccg atgaatcaag
     4921 acggcagcat ccattacgaa caactccgcg acttaatcga ctggcacatt gaaaacggca
     4981 cggacggcat cgttgccgtc ggcacgacag gcgaatccgc caccctctcc gtcgaagaac
     5041 acaccgccgt catcgaagcc gtcgtcaaac acgttgccaa acgcgtcccc gtcatcgccg
     5101 gcacaggggc aaacaacacc gtcgaagcca tcgccctttc ccaagccgcc gaaaaagccg
     5161 gggcggacta caccctctcc gtcgtcccct attacaacaa accttcgcaa gaaggcatgt
     5221 accgccattt caaagccgtt gccgaagccg ccgcgattcc gatgatactc tacaacgtac
     5281 ccggcagaac cgtcgtcagc atgaacaacg aaaccatcct gcgccttgcc gaaatcccca
     5341 acatcgtcgg cgtgaaagaa gccagcggca acatcggcag caacatcgaa ctcatcaacc
     5401 gcgcgcccga aggcttcttc gtcctttccg gcgacgacca taccgccctg ccgttcatgc
     5461 tgtgcggcgg acacggcgtg attaccgtag ccgccaacgc cgcgcccaag ctctttgccg
     5521 atatgtgccg cgccgcgctt caaggcgata tcgcccttgc acgcgaactc aacgaccggc
     5581 tgattccgat ttacgacacc atgttctgcg aacccagccc cgccgcaccg aaatgggcgg
     5641 tatccgccct gggcagatgc gaaccgcacg tccgcctgcc gctcgttccg ctgacggaag
     5701 gcggacaggc aaaagtccgc gccgccctga aagcctcagg acaactttaa atatagccat
     5761 gccgtctgaa gcgttcagac ggcataacgc aacaaacact ttaatatcca tcacacagga
     5821 tgacacgatg acccatatca aacccgtcat tgccgcgctc gcactcatcg ggcttgccgc
     5881 ctgctccggc agcaaaaccg aacagcccaa gctcgactac caaagccggt cgcaccgcct
     5941 gatcaaactc gaagtcccac ctgatttgaa caaccccgac caaggcaacc tctaccgcct
     6001 gcctgccggt tcgggcgccg tccgcgccag cgatttggaa aaacgccgca cacccgccgt
     6061 ccaacagcct gccgatgccg aagtattgaa aagcgtcaaa ggtgtccgcc tcgagcgcga
     6121 cggcagccaa cgctggctcg ttgtcgacgg caagtctcat gccgaaatct ggccgctcct
     6181 gaaagccttt tggcaggaaa acggcttcga catcaaatcc gaagaacccg ccatcggaca
     6241 aatggaaacc gagtgggcgg aaaaccgtgc caaaatcccc caagacagct tgcgccgcct
     6301 attcgacaca gtcggtttgg gcggcatcta ctccaccggc gagcgcgaca aattcatcgt
     6361 ccgtatcgaa cagggcaaaa acggcgtttc cgacatcttc ttcgcccaca aagccatgaa
     6421 agaagtgtac ggcggcaaag acaaagacac gaccgtatgg cagccctccc cgtccgatcc
     6481 caacctcgaa gccgctttcc tgacgcgctt tatgcaatat ttgggcgttg acggacagca
     6541 ggcggaaaac gcatcggcaa aaaaaactac ccttcccgcc gccaacgaaa tggcgcgtat
     6601 cgaaggcaaa agcctgattg tctttggcga ctacggcaga aactggcggc gcaccgcgct
     6661 cgccctcgac cgcatcgggc tgaccgtcgt cggtcaaaac accgaacgcc acgctttcct
     6721 ggttcaaaaa gccccgaacg aaagcaatgc agttaccgaa caaaaacccg gcctgttcaa
     6781 acgcctgctg ggcaaaggca aagcggagaa acctgccgaa cagccggaac tgattgtcta
     6841 tgccgagcct gtcgccaacg gctcgcgcat cgtcctgctc aacaaagacg gcagcgcata
     6901 tgccggcaaa gacgcatccg cattattggg caaactccat tccgaactgc gttaaaccat
     6961 tcaaaacaaa agcagcttcc aaacagaagc tgctttttta tgcggaactt tttacaacaa
     7021 atggggcaaa atatcctcaa ctgcctgaac caacgcatcc accaaaggcg gatcgaacgc
     7081 acgatgccct gcctgaacca ccctcaattc cgcttcggga aaggctttcg acagcgccca
     7141 cgcactctgc atcggcgtac acaaatcata ccgcccctgc acgataatgg tcgggatatg
     7201 ccgtattttg ccgatattgt tcaaaatcgc cctatcgccc tgcaaccagc cgccgttgac
     7261 aaaataatgg ttttccaaac gcgcgattgc cagtgaggca taagcatctt catccacttc
     7321 ctccggctcg aaacggatca gatagctttc ccaatccgcc caagcctttg cggcagacag
     7381 gcacacttct tcatcttgat gaaacagcaa tccgtgatac gcctcaatca gccggttccg
     7441 ccgattttca gcaatcggcg cgacaaattt ttgccattgt tccggataaa tccggctcac
     7501 accgcccgcc tcgttcagcc acgccgtttc agacggcctg cacaaaaata tcccgcgcaa
     7561 taccaatccc tttacccgtt caggatgggt ttgggcataa gccagcgaca aagtgctgcc
     7621 ccacgaaccg ccgaacacca gccatttcct gatacccagc atttcacgga ctttttcaat
     7681 atccgccacc aaatcccaag tcgtattgtc ttccgcacaa gcatacgggc gtgaacgtcc
     7741 gcaaccgcgc tggtcgatga tgacgatgcg gaacacatcg ggattgaaaa aaccccgaca
     7801 ttcaggcgaa gcccccgcgc ccggtccgcc gtgcaggaaa atgaccggca caccgtcggg
     7861 attgcccgac tcttcccaat aaatttgatg aatttcagac acctgcaagt atccgctatg
     7921 aaaaggctgt tttatctcat acatcatctt ctcctatatt gcttctgaca atccgaacat
     7981 cccgacacgc gacatcccgc acaaaacaca agccgccccg aaaggcaaat tccgaaaaat
     8041 cgcgatatag tggattaaca aaaatcagga caaggcgacg aagccgcaaa cagtacaaat
     8101 agtacggaac cgattcactt ggtgcttcag caccttagag aatcgttctc tttgagctaa
     8161 ggcgaggcaa cgctgtaccg gtttttgtta atccgctata ttccgccatc tctaagattt
     8221 acagcgatac acgggtgatt taaggaatgc ccgaaccgtc a
//
LOCUS       DAWFIE010000083         8233 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWFIE010000083 DAWFIE010000000
VERSION     DAWFIE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.083"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(173..589)
                     /gene="dksA"
                     /locus_tag="WGQ71_001740"
     CDS             complement(173..589)
                     /gene="dksA"
                     /locus_tag="WGQ71_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ0707708.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDEHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(736..1527)
                     /locus_tag="WGQ71_001741"
     CDS             complement(736..1527)
                     /locus_tag="WGQ71_001741"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ0707709.1"
                     /translation="MNVYFLGGGNMAAAIAGGLVKQGGYRIYIANRGAEKRERLEKEL
                     GVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVDTLSRYLGG
                     NRRIVRVMPNTPGKIGLGVSGMFAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1576..1983)
                     /locus_tag="WGQ71_001742"
     CDS             complement(1576..1983)
                     /locus_tag="WGQ71_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ0707710.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSERVRW"
     gene            complement(1983..2669)
                     /locus_tag="WGQ71_001743"
     CDS             complement(1983..2669)
                     /locus_tag="WGQ71_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ0707711.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGREPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSEQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLSDLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2789..3832
                     /locus_tag="WGQ71_001744"
     CDS             2789..3832
                     /locus_tag="WGQ71_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ0707712.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3995..5221
                     /locus_tag="WGQ71_001745"
     CDS             3995..5221
                     /locus_tag="WGQ71_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0707713.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5507..7657
                     /gene="yccS"
                     /locus_tag="WGQ71_001746"
     CDS             5507..7657
                     /gene="yccS"
                     /locus_tag="WGQ71_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ0707714.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESSVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIIGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         2035 a         2696 c         1762 g         1740 t
ORIGIN      
        1 gtatacggtc aatacttcat cttgcttttt ttaattattt tctccatctc gttgtctgat
       61 aaggtttttt atttcgccac aaaccacatc cgactcgaaa tttacctgcc gccatcttaa
      121 tctgcttccc tttttttctg tcatatggtt tgaaaataac caaaaggcaa atttagtcgg
      181 caaactgttt tttcatcctc tctcggcgtt cttgggactc aacagataaa gtggctgtcg
      241 ggcgtgccag cagccgcttc agaccgatag gctctcccgt atcggcacag aatccataat
      301 ccccttcatc aatattgcgg atggtcgcct gtattttact gagaagtttt cgttcccgat
      361 cgcgggtacg gagttccaat gcgtactctt cttcctgtgt ggcacggtcg gcaggatcgg
      421 gggctgattc gtgttcttgg agatgccctg tcgtagcgga agcattttcg atgagttcgt
      481 cttgcatttt taccagcaat tcgcggaaaa aagccaaatg ctcgtcattc atataatcgt
      541 cttccggccc gttccaattc aaaatatctt gttctgtcag ctttgccata atgtatatcc
      601 tctcggttca ccgaaaaaaa caaagcacca caaagtggct ttattccttt gatttttata
      661 aggcttattg cctaaggcgt ttttgaaaac gcgcatcata acacgttttg gattggtttt
      721 tttattttct ttacattatt gatattgccg ttccatttcc tgcgaacggc gcacacaggc
      781 acaaacgccc tcacttatgg cttcggcgac acgatgccgc ctgaaggctt ccacggcttc
      841 gtgggttgtc ccgccttttg acgttacatt tttttgaagc ttctcgaaat cttcacccgt
      901 ctgctcggca agggcaaccg ctcctttaaa cgttgccaga ctgagcgcgc gtgcttctgc
      961 catatcaaac ccttgtcgga tggcggcatt ttgcaatgcg tccagcagat aaaacacata
     1021 tgccggcccg ctgccgctga taccggtaat gctgtgcatt tgcgcttcat cttccaacca
     1081 aacggtcaaa ccgactgatt tcatgattcg atcggcaatc ctgcggtctg tttccgatac
     1141 ttccgcttcg gcaaacatac cggatacgcc cagcccgatt tttccgggtg tattcggcat
     1201 aacccggaca atgcggcggt ttcccccgag gtaacggctg agcgtatcaa ccgacaatcc
     1261 agctgcaaca gaaagcacca atgcgccgtt ggtgcggata tttttgcacg cggcttccat
     1321 atcctgcggt ttgacggcaa ggattaaaac atcgtcggaa tgaagctccg gcagggttgc
     1381 cgaagtttcg acccccaact ctttttccaa acgttcgcgt ttttccgcac cccgattggc
     1441 tatatagatg cggtaaccgc cttgtttgac caatccgcct gcgatggcag ccgccatatt
     1501 gccgccgccg agaaaataaa cattcattat ttctacccta ttcaaaatta ccttactaaa
     1561 ttcattccca attttttacc atcggacgcg ctcggacaga ccgttgcgcc gcaacccctg
     1621 ctttttcaaa caatcaaaac cttcctgttt gaacgtctca atcaaaggtt tgccatcacg
     1681 cgtacgccaa tccaacccgc aggcttcaaa cttccctatt ttcctgtaaa agtaagattg
     1741 ttgtgcggat tcatattcgt ttgccctgac ggaacgattg ccgtctgaaa gcggtatctc
     1801 gtagtcctct aaagacggcg gactctcgcc gccaatatcc caaaaatcga tggcggcagg
     1861 ttttctcggc ttacaccagc cggacaggga cgaacactca taccaacctg ccaaccggca
     1921 gcctgcaacc gtaaatgcaa gcaacgcagc cattgccgta tattttattg cccgaatttc
     1981 catcagcccc ttttcccgaa aatcgcgctg ccgatacgga cgtgtgtcgc accgcactca
     2041 atggcggcag gcatatcgtc cgacatcccc atagacagca cgtccgcctt aacgccagcc
     2101 gtattgaggt cggaaagcag tttccgcatc gtttgaaatt gcaccttcaa ctccgtttca
     2161 ctgctgttgg ctttggcaac acacatcaag ccgcgtacga cgatattcgg cagcttcgcc
     2221 acttccacag caagcgcgac tgcttcttcg ggcgcaacac cgtgctttgc cgcctcgccc
     2281 gcaatattca cctcgataca cacctgcaaa ggcggcattg aggaaggacg ctgttcgctc
     2341 aaccggacag cggttttcag acggcatacg gtatgcaccc aatgcgcgcg ttcggcgaca
     2401 aacttggttt tgttggactg cacatcgccg atgacgtgcc acacgatgtc ggtcaaatcc
     2461 gccaactctt ccgttttgcc gtaccactcc tgaatatagt tctcgccgaa atcacgctgt
     2521 ccggcggcgt aaacttcgcg gatgccgtct gaagggaaag tcttaccgac ggcaatcagg
     2581 ctgacggaat gcggctccct gcccgcctgc agaaccaatt ttccgatacg gtcggacacc
     2641 tcacaataac gttcttgcaa caccgtcata gattatcccc taattaaaaa tgattaaaca
     2701 gttgaaaccc ctccagtcag ggcggtacaa tcaaggttgt tagaaccatt ccaaccaatc
     2761 gaaacattat actaaacaga gccgcattat gcagattacc gacttactcg ccttcggcgc
     2821 taaaaacaaa gcatccgacc ttcacctgag ttcgggcata tcccctatga ttcgggttca
     2881 cggcgatatg cggcgcatca accttcccga aatgagcgcg gaagaggtcg gtaatatggt
     2941 aacttcggtg atgaacgacc accagcggaa aatctaccag caaaacttgg aagtcgactt
     3001 ctcgttcgaa ctgcccaacg tcgcccgatt ccgcgtcaac gccttcaaca tcggccgcgg
     3061 tcccgccgcc gtattccgca ccattcccag caccgtctta tcgctggaag aattgaaagc
     3121 cccgagcatt ttccaaaaaa tcgcagaatc gccgcgcggc atggttttgg ttaccggccc
     3181 taccggttcg ggcaaatcga ccacgcttgc cgcaatgatc aactacatca acgaaaccca
     3241 gccggcacac atcctgacca tcgaagaccc gatcgaattc gtccaccaaa gcaaaaaatc
     3301 cctgattaac cagcgcgagc tgcaccagca caccctcagc ttcgccaacg cgctgcgttc
     3361 cgcattgcgc gaagaccccg acgttatcct tgttggcgag atgcgcgacc ccgaaaccat
     3421 cggcttggca ctgaccgccg ccgaaaccgg acacttggtt ttcggcacgc tgcacacgac
     3481 cggcgcagca aaaaccgtcg accgtatcgt ggacgtattc ccagcgggag aaaaagaaat
     3541 ggtgcgctct atgctgtccg aatcgctgac cgccgtcatc tcccaaaacc tgctgaaaac
     3601 gcacgacggc aacggccgtg tcgcctcgca cgaaatcctg attgccaacc ccgccgtccg
     3661 caacctcatc cgcgaaaaca aaatcacgca gattaactcc gtcctgcaaa ccgggcaggc
     3721 gagcggtatg cagacgatgg accaatcgct gcaatcgctg gtgcgccaag ggctgatcgc
     3781 accggaagtc gcacgcagac gcgcgcaaaa cagcgaaagt atgagtttct gacacacaac
     3841 cgccttccgg ccataccggc gggaaaacaa ggcgcaaaca cgcggggcgg gacgcagcat
     3901 cccgcccggc tacctttccg aacaggaagc gtccgccttc ctgttgaaac ctgccgccgc
     3961 aaactgcaag gcttaaaccg aaaagaagct aacgatgaat accgataacc tgcacgacat
     4021 cttggacgaa atggttcaag tgtattctca aaaaaaacaa agccgatccg aaaccccggc
     4081 cgaaatcggc gcacacttcc acccgctgct cgaccgcctg tgcgaaaccg cagaagcaca
     4141 aaacgcgtcc gacatcctta tcagcaaagg attcccgccc tcgttgaaaa tcaacagcgc
     4201 attaaccccg cagccgcaaa aggcgctgac gggcgaggaa accgccgcca tcgccgcatc
     4261 gacgatgaac gccgaacaat cggaaatatt ccggcgcgac ggcgaaatca actactccgt
     4321 ccagtcgcgc agcggcacgc gctaccgcgc caacgcctac cacagccaag gcagcgcagg
     4381 tttggttttg cggcgcatca accacgtcat cccgcaaatg caggaattgg gcctgcccga
     4441 aaaactcaaa gacctcgccg tcgcaccgcg cgggctgctg attatcgtcg ggcctaccgg
     4501 ttcgggcaaa tccaccacga tggcgactat gctcgaacac cgcaacaaaa ccctgcccag
     4561 ccatatcgtt accatcgaag acccgattga gtttatctac aaaccgcgcc gctgcatctt
     4621 tacccagcgc gaaatcggcg tcgacaccat aaactggcag acggcggtac aaaacgctat
     4681 gcgccaatcc cccgacgtgg tctgcatcgg cgaagtccgc agcagggaaa gtatggaata
     4741 cgcgatgcag ctcgcccaaa ccggccacct gtgcattttt acgctccacg ccaacaccgc
     4801 gccgcagtcg ctcgaacgca tactcaactt ctaccccaaa gaacagcaca accaaatact
     4861 gatcgacatc gccctcaacc tgaccggcat catctgccaa cgcctcgccc tcaaacaaga
     4921 caaaacgggc aggacggcgg ttgtcgactt gctcatcaac acgcccgcca tccaagactt
     4981 catcctgaag ggcgacctga tgaacatcag taaaatcatg gaaaccgcca aaaccgacgg
     5041 aatgcagacg atggatcaaa accttttcga actgtaccgt cacggcatca tcagttacga
     5101 agaagccctg cgccagtccg tttccgccaa caacctgcga ttgcacatcc aactgcacaa
     5161 agaaggcaaa acgcccgaac tcctttacga cagggtcaac ggtctcaacc tcatttcctg
     5221 atccgcaaaa cccaatgccg tctgaaaacc gcatccccgt tttcagacgg catgatttta
     5281 tccgccccat tcatgtgcta cactttatag tggattaaat ttatagtgga ttaacaaaaa
     5341 tcaggacaag gcgacgaagc cgcagacagc acaaatagta cggaatcgat tcacttggtg
     5401 cttgagcacc ttagagaatc gttctctttc agctaaggcg aggcaacgcc gtactggttt
     5461 ttgttaatcc actatattca cttcataaca ataaaccggt aaaaccatga aaaccccact
     5521 cctcaagcct ctgctcatta cctcgcttcc cgttttcgcc agtgttttta ccgccgcctc
     5581 catcgtctgg cagctaggcg aacccaagct cgccatgccc ttcgtactcg gcatcatcgc
     5641 cggcggcctt gtcgatttgg acaaccgcct gaccggacgg ctgaaaaaca tcatcgccac
     5701 cgtcgccctg ttcaccctct cctcgctcac ggcacaaagt accctcggca cagggctgcc
     5761 cttcatcctc gccatgaccc tgatgacctt cggctttacc attttaggcg cggtcgggct
     5821 gaaataccgc accttcgcct tcggcgcact cgccgtcgcc acctacacca cacttaccta
     5881 cacccccgaa acctactggc tgaccaaccc cttcatgatt ttgtgcggca ccgtactgta
     5941 cagcaccgcc atcatcctgt tccaaatcat cctgccccac cgccccgtcc aagaaagcgt
     6001 cgccaacgcc tacgacgcac tcggtggcta cctcgaagcc aaagccgact tcttcgaccc
     6061 cgatgaggca gcctggatag gcaaccgcca catcgacctc gccatgagca acaccggcgt
     6121 catcaccgcc ttcaaccaat gccgttccgc cctgttttac cgccttcgcg gcaaacaccg
     6181 ccacccgcgc accgccaaaa tgctgcgcta ctacttcgcc gcccaagaca tacacgaacg
     6241 tatcagctcc gcccacgtcg actaccaaga gatgtccgaa aaattcaaaa acaccgacat
     6301 catcttccgc atccaccgcc tgctcgaaat gcagggacaa gcctgccgca ataccgccca
     6361 agccctgcgc gcaagcaaag actacgttta cagcaaacgc ctcggccgcg ccatcgaagg
     6421 ctgccgccaa tcgctgcgtc tcctttccga cggcaacgac agccccgaca tccgccacct
     6481 gcgccgtctt ctcgacaacc tcggcagcgt cgaccaacaa ttccgccaac tccagcacaa
     6541 cggcctgcag gcagaaaacg accgcatggg cgacacccgc atcgccgccc tcgaaaccgg
     6601 cagcctcaaa aacacctggc aggcaatccg tccgcagcta aacctcgaat caagcgtatt
     6661 ccgccatgcc gtccgcctgt cccttgtcgt tgccgccgcc tgcaccatcg tcgaagccct
     6721 caacctcaac ctcggctact ggatactact gaccgccctt ttcgtctgcc aacccaacta
     6781 caccgccacc aaaagccgcg tccgccagcg catcgccggc accgtactcg gcgtaatcat
     6841 cggctcgctc gtcccctact ttaccccctc cgtcgaaacc aaactctgga tcgtcatcgc
     6901 cagtaccacc ctctttttca tgacccgcac ctacaaatac agtttctcca ccttcttcat
     6961 taccattcaa gccctgacca gcctctccct cgcaggtttg gacgtatacg ccgccatgcc
     7021 cgtacgcatc atcgacacca ttatcggcgc atcccttgcc tgggcggcag tcagctacct
     7081 atggccagac tggaaatacc tcacgctcga acgcaccgcc gcccttgccg tatgcagcaa
     7141 cggtgcctat ctcgaaaaaa tcaccgaacg cctcaaaagc ggcgaaaccg gcgacgacgt
     7201 cgaataccgc gccacccgcc gccgcgccca cgaacacacc gccgccctca gcagcaccct
     7261 ttccgacatg agcagcgaac ccgcaaaatt cgccgacagc ctgcaacccg gctttaccct
     7321 gctcaaaacc ggctacgccc tgaccggcta catctccgcc ctcggcgcat accgcagcga
     7381 aatgcacgaa gaatgcagcc ccgactttac cgcacagttc cacctcgccg ccgaacacac
     7441 cgcccacatc ttccaacacc tgcccgaaac cgaacccgac gactttcaga cagcactgga
     7501 tacactgcgc ggcgaactcg acaccctccg cacccacagc agcggaacac aaagccacat
     7561 cctcctccaa cagctccaac tcatcgcccg gcagctcgaa ccctactacc gcgcctaccg
     7621 acaaattccg cacaggcagc cccaaaacgc agcctgaaaa agtttcggca ttttgtaaga
     7681 gagggcagat tgtcagacag gttgcaagat agtggataaa gctttgcgca gggtaaatgc
     7741 gtagcaactg aaccgtcatt cccacgaacc tacatcccgt cattcccacg aaaacagaaa
     7801 accaaaaaca gaaacctaaa atcccgtcat tcccacgaaa gtgggaatcc agttcgttcg
     7861 gtttcgcttg ttttaagttt cgggtaactt ccacttcgtc attcccgcgc aggcgggaat
     7921 ccagtgcgtt gagcttcagc tatttagaat aaattttgaa actctaatcc cgtcattccc
     7981 acgaacctac attccgtcat tcccacgaaa gtgggaatcc agaatcccgg actttcagat
     8041 aatctttgaa tattgctgtt gttctaaggt ctagattccc gcctgcgcgg gaatgacgaa
     8101 tccatccgca cggaaacctg caccacgtca ttcccacgaa cctacatccc gtcattccca
     8161 cgaaagtggg aatccagttt tttgagtttc agtcattccc gataaattgc cttagcattg
     8221 aatgtctaga ttc
//
LOCUS       DAWFIE010000084         8153 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWFIE010000084 DAWFIE010000000
VERSION     DAWFIE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.084"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            123..1046
                     /locus_tag="WGQ71_001747"
     CDS             123..1046
                     /locus_tag="WGQ71_001747"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ0707715.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFLRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
     gene            2225..3673
                     /gene="gnd"
                     /locus_tag="WGQ71_001748"
     CDS             2225..3673
                     /gene="gnd"
                     /locus_tag="WGQ71_001748"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ0707716.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLDEEDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWDAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLGNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            3736..5007
                     /gene="waaA"
                     /locus_tag="WGQ71_001749"
     CDS             3736..5007
                     /gene="waaA"
                     /locus_tag="WGQ71_001749"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ0707717.1"
                     /translation="MFQWLYDALWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHTVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKALSSEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            5048..5497
                     /locus_tag="WGQ71_001750"
     CDS             5048..5497
                     /locus_tag="WGQ71_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ0707718.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            5530..6504
                     /locus_tag="WGQ71_001751"
     CDS             5530..6504
                     /locus_tag="WGQ71_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0707719.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNSSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLSFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            6573..6648
                     /locus_tag="WGQ71_001752"
     tRNA            6573..6648
                     /locus_tag="WGQ71_001752"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6606..6608,aa:Lys,seq:ttt)
     gene            6867..8120
                     /gene="murA"
                     /locus_tag="WGQ71_001753"
     CDS             6867..8120
                     /gene="murA"
                     /locus_tag="WGQ71_001753"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ0707720.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMVVEGA
                     GELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
BASE COUNT         1909 a         1980 c         2288 g         1976 t
ORIGIN      
        1 atttttatgc ggcttacgta ttgaaaacgg atacattggc aaatactatg gaaaaacata
       61 gcttgttttt ccggttttat cgtattattc caaaacttac cccattgata aggaagcggt
      121 ttatgctgca aagaactttg gcgaaatcca tcagcgttac cggagtcggg ctgcattcgg
      181 gcgaacgcgt cgcgctgacc ctgcaccccg cgcctgaaaa cagcgggatt tccttcctac
      241 gcaccgattt ggacggcgag atgggcgaac aaatcaagct gaccccttat ttgatcaacg
      301 atacccgcct ttcatccacc atcgttaccg acaaaggcgt gcgcgtcggc acgatcgaac
      361 acattatgtc cgcgctgtcc gcctacggta tcgacaacgc actgattgag ctgaacgcgc
      421 ccgaaatccc gattatggac ggctccagcc tgccgtttat ttaccttttg caagatgcgg
      481 gcgtggtcga tcaaaaggcg caaaagcgtt ttttgaaaat cctcaagcct gtcgaaatca
      541 aagaagcggg caaatgggtg cgctttacgc cgtatgacgg ctttaaggta acgctgacca
      601 tcgaattcga ccatccggtt ttcaaccgca gctcgcccac ttttgaaatt gatttcgccg
      661 gcaaatccta catcgacgaa atcgcgcgcg cgcgcacttt cggctttatg cacgaagtgg
      721 aaatgatgcg cgcccacaat ctgggtttgg gcggcaattt gaacaacgcc atcgtgattg
      781 acgacacgga tgtcctgaat cctgaaggct tgcgctatcc cgatgagttt gtgcgccaca
      841 aaatccttga tgccatcggc gatttgtata tcgtcggaca tccgattgtc ggcgcgtttg
      901 aaggctacaa atcgggacac gccatcaaca acgcactctt gcgcgcggtt ttggcagacg
      961 aaacggctta cgaccgggtg gaatttgccg acagcgatga tttgcccgac gcatttcacg
     1021 agctgaacat cagaacttgt ggataacgct ttctattttt tccggtaaat tcctaaaact
     1081 taaaatttca tcattcctac aaaaacagaa aaccaaaatc agaaacctaa aatcccgtca
     1141 ttcccacgaa agtgggaatc tagttttttg agtttcagtc atttccgata aattgcctta
     1201 gcattgaatg tctagattcc cgctttcgcg ggaatgacgg cggagcggtt tctgtttttg
     1261 gtttttgtgg gaatgacggg atgtaggttc gtaggaatga cgtggtgcag gtttccgtat
     1321 ggatggattc gtcattcccg cgcaggcggg aatctagaac gtaaaatcta aagaaaccgt
     1381 gctgtaaacg gcagactgat gccgtcattc ccacgaaagt gggaatccag aatctcggac
     1441 tttcagataa tctttgaata ttgctgttgt tctaaggtct ggattcccgc ctgcgcggga
     1501 atgacggcgg agcggtttct gttttttccg ataaatgccg caatctcaaa tcccgtcatt
     1561 cccactttcg tgggaatgac ggaatgtagg ttcgtgggaa tgacgcggta caggtttccg
     1621 ttacgtatcc ccatccgaaa gtttatttat acacaagcaa tccaaagtta tctgactgaa
     1681 atattgtgag atttacccgc aaatctcatt ttttccacca cttatacaca aattcggatg
     1741 cgcactctgc cgatatgcag aaataagaca ttcgtgccga cacctgacca tttccacaag
     1801 ttccaaacac actttcctct caaacctcca cagtattccg gttcttaaag acatcggcag
     1861 ccattcggta aagaaaagcg gaatgctatc tgcacaacag gcaaactgtc cacaaaaact
     1921 attcttcatg aaaaacaatc cacaggtatg cacaaaaggg aatccgcctt accgacaccc
     1981 ttttaaaacg gaaatcccaa tgatttttaa aataaaaaaa agttacccac agaaaatttc
     2041 tcctatcaat ataatcatcg tttttttatt taatttttat attttattta taagccttca
     2101 gggaaacgca cggtaaagcg taaaggcatc ttttcaaaaa attaaaaagt taaggattgt
     2161 taaacaagtg taaatcaggt agagttgcgg tcgataaatt aaatctataa cggagcttcc
     2221 tgcaatgaaa ggcgatattg gcgtaatcgg tttggcggtt atggggcaaa acctgatttt
     2281 gaatatgaac gattgcggat ttaaggttgt cgcctacaac cgaacaatcg gtaaggtgga
     2341 cgaattttta aacggcgcgg ctaagggaac ggatattgtc ggcgcgtatt ccctgcaaga
     2401 cttggtcgat aagctggcaa aaccgagaaa aatcatgatg atggttcgcg caggttcggt
     2461 ggttgacgac tttatcgaac aactgctgcc cctgttggac gaggaagaca tcattatcga
     2521 cggcggcaat gccaattacc ccgacacgac acggcggaca cattaccttg ccgaaaaagg
     2581 gattttgttt gtcggggcgg gcgtatccgg cggcgaagaa ggcgcgcggc acggcccgtc
     2641 cattatgccg ggcggggata agcgcgcttg ggatgcagtc aagccgattt ttcaggcgat
     2701 tgccgccaaa acgccgcagg gcgaaccgtg ttgtgactgg gtcggcaaag acggtgcggg
     2761 acattttgtc aaaatggtgc ataacggtat cgaatacggc gatatgcagt tgatttgcga
     2821 agcgtaccag ttcatgaaag atggtttggg attgtcctac gatgaaatgc accgcgtgtt
     2881 tgccgagtgg aacaaaaccg agctggattc ttatctgatt gaaatcacag cggcaatttt
     2941 gggctataag gacgaaggcg gtgaaccgct ggtcgagaaa atcctcgata cggcagggca
     3001 aaaaggcacg ggcaaatgga cgggcatcaa tgccttggat ttgggtattc cgttgacgct
     3061 gatttccgaa gcggtattcg cccgctgcgt ttcgtcgttc aaagaacagc gcgtgcagac
     3121 cggcaagctg ttcgcccgaa ccgttacgcc cgtcgaaggc ggcaaacaag aatgggttga
     3181 ggcgttgaga caggccctgc ttgcatccaa aatcatttcc tacgcacaag gctttatgct
     3241 gatccgcgaa gcgggcgaaa gctacggctg ggatttggac tacggcaaca ctgcgctgct
     3301 gtggcgcgag gggtgcatca tccgcagcgc gtttttgggc aatatccgcg acgcgtatga
     3361 aaacaatccc gatttggtgt tcttgggtgc ggatccgtat ttcaaaaata ttttggaaaa
     3421 ctgcctgccg gcgtggcgca aggtggttgc caaggcggtc gaatgcggca tccctatgcc
     3481 ctgcatggct tcggcgatta cgttcttgga cggctacacg accgaacgtc tgcctgccaa
     3541 cctcttgcag gcacagcgcg actacttcgg cgcgcatact tacgagcgca ccgacaaacc
     3601 gcgcggcgag tttttccata ccaactggac gggcaagggc ggagataccg cttcgacaac
     3661 ctacgatatt tgattccgtt gccgaaatgc cgtccgaacc gttcggacgg catttgtttt
     3721 gcaggaaata gaaagatgtt ccaatggctt tatgatgcat tgtggctgct tgcgccgata
     3781 tggatacggc gttatttgga caaacgctcc ggaagtgccc cggcatatcg ggcgcatcgg
     3841 gacgagcgtt tcggcaagcc gtatccgaat cccgttaccg gagcggtttg gatacacacc
     3901 gtttcggtcg gggaaacgcg tgccgcccaa cctttgatac gcgagttgcg gcagcgtttt
     3961 cccgatgcgc cgctgctgat gacgcagatg accccgacgg gacgggaaac cgcgcaagtt
     4021 ctgtttcctg atgcacaatg ccgctatctt ccgtatgaca aaaaaacgtg ggtacggcag
     4081 tttttgcgcg aacaccgccc gatgttcggc attttgatgg aaaccgaaat ctggcccaac
     4141 ctgatgaagg aatgccggcg cgcgggtgtg ccgctgtttt tggcgaatgc gcggctgtcg
     4201 gaaaaatcgt tgaacggtta tctgaaagtc cgccgcctga tccgtcctgc cgccgcttcg
     4261 ctgacgggtt gtctggcgca gacggaggcg gatgcggcgc ggttggcgaa attgggcgcg
     4321 gcatccgtgc aggtgtgcgg caataccaaa tacgacctga tgccgtcgga acagatgaaa
     4381 acgctggcgg ggcagtttga aaaacgcatc ggaggcagac cggttgccgt gtgcggcagc
     4441 acgcgcgttt atcggggcga agacgaggcg gagaaactgc tggcggcgtg gcaacaatat
     4501 cgcggcgatg ccctgctggt cgtcgtgccg cgccatcccg agcattttca gacggtattt
     4561 gaaacggcaa aacgcttcgg gtttaaggtt cagcggcgca gcgacggttt gccggtcgaa
     4621 cctgatacgc aggtgtggat aggcgacagt atgggcgagc tgtatgcgta ttacctgtgc
     4681 gccgatgtcg cttttgtcgg cggcagtctg gtcgattcgg gctgtcagaa cattatcgaa
     4741 ccgctttcct gcggcgttcc gacgatattc ggcttttcga cctacaattt ttccgaagcc
     4801 tgccggcacg ccttggcatc gggtgcggcg gttcaagttg aatcggcgga tgcgtggcgg
     4861 gaagccgttg aaaaagcctt atcgtccgag gggggcggaa tgcagatgca ggcacgcgtg
     4921 gacggcttta tcgcgcaaca tcgcggagcg ggcgcgagaa tcgccgaggc ggtacgggaa
     4981 gcggtatgcg gacatcgggg acgatagtga tacaatccgt atccgagttt tccggttgga
     5041 gtaccgtatg agtatttatg ccgtcgcgca catcgtccac ctgtattgcg ccatcgcctt
     5101 tgtcggcggc gtgttttttg aagtgctggt tttgtccgtc ctgcatacgg gacgggtgtc
     5161 gtgcgaggcg cggcgcgaag tggaaaaggc aatgtcttac cgcgccgtca gggtgatgcc
     5221 gtttgtggtc ggactgctgt tcgccagcgg catcgtgatg gcggcaaacc gctatctttc
     5281 tatattgggc gaaccgtttg ccacttcctt cggtacgatg ctgacgctga aaatcctgtt
     5341 ggcgttcagc gtgttggcgc acttcgccat cgccgtcgtc aaaatggcgc gttccacact
     5401 gaccgtcggc tggtcgaaat acatacacac cgtcgtcttt acccatatgc tgctgattgt
     5461 ctttttggca aaagcgatgt tttatatcag ctggtaacac aactacattt caaatcagga
     5521 gattgaacca tgacagaata tcccggcatc gggtcgccgt tgttttacgg cgtttttttt
     5581 gcggcagtgc tggtcatgat tgccttggat atgttttcgc tgaagaaaaa cagcagccat
     5641 aaagtcggcg tcaaagaagc cttggcatgg agcggcttgt gggttgccgt atcctgcctg
     5701 ttcgcgggct ggctgtattt tgaactcgcc ggcaatctcg gctacggcgc ggcagtcgca
     5761 aaagaaaaag tattggaatt ctttaccggc tacattttgg aaaaatcgtt ggcagtcgac
     5821 aatattttcg tgttcctgat gatattcggc tacttcaaag tcgcaccaca gtttcagcac
     5881 cgcgtgctgc tgtacggcgt attgggcgca ttggtattgc gcactgtcat gattttcgtc
     5941 ggcgcggcac tggttcagca gtttgagtgg attctgtatc tgtttggcgc gttcctgctc
     6001 tatactggta tacacatgat gaagcccgaa ggcgatgaga aagaggattt ggcaaacagc
     6061 aggctgctga atgccgtcaa gaaagtcgtt ccggtcggca cggaatttca cggcgagaaa
     6121 ttttttaccg tcgaaaacgg caaaaaaatc gccacgccgc tgtttttggt gctggtcatg
     6181 attgaattga gcgatgtcgt gtttgccgtg gacagtattc ccgccgtctt tgctgttacc
     6241 accgatccgt ttatcgtgct gacctccaat attttcgcca ttttgggttt gcgggcgatg
     6301 tatttcctgc tggcggatgt ggcggaacgc tttatcttcc tgaaatacgg cttggcattc
     6361 gtgttgagtt ttatcggtgt gaaaatgctg gtaatgcatt gggtgcatat cccgatttcc
     6421 gtttcgctgt cggtcgtgtt cggcgcgttg ggtgcatcga tactgacctc gttaatttat
     6481 acgaaaaaac agcctgataa ataaaatgcc gtctcaacgc cgacaaaatg gttgacagca
     6541 tcaggcttga tgtatagaat ttcgagcttg tcgggtcgtt agctcagccg gtagagcagc
     6601 ggacttttaa tccgttggtc gagcgttcga atcgctcacg acccaccaga taaaacaaaa
     6661 aaagctgagt tttcaaaact cggctttttt gtcttccgac aatccttgtt cgaaaagcgc
     6721 ggcatttcgt caaattcttt ggtgcggctt ccgtattttt cccgaaaatc ccttataatt
     6781 ccgcctctgc aaccttcatc gggggatgct ttatgcgttg tcccgagtat tgaaatatta
     6841 caagtaccca ccaggaaaga acaaacgtgg acaaactgaa aatctccgca aacggcccgc
     6901 tcaacgggga aataacggtc tcgggcgcga aaaacgcggc attgccgctg atgtgcgcgg
     6961 gtttgctgac atcgggtacg ttgcgcctga aaaacgtccc tatgctggca gatgtgaaaa
     7021 ccacgcaaaa gctgcttcag gggatgggcg cgcgcgtcct gaccgacaat atcagcgaat
     7081 ttgaaatcaa cggcggtacg gtaaacaata cctgcgcccc ttacgaattg gtacggacga
     7141 tgcgggcctc gattttggtg ctggggccta ctttggcacg tttcggcgag gcgcaagtca
     7201 gcctgccggg cggctgcgcc atcggttcgc gccccgtcga tcagcatttg aaaggcttgg
     7261 aagcgatggg tgctgagatt gttatcgaac acggttacgt caaagccaaa ggcaaactca
     7321 aaggtacgcg cgtggcgatg gatgtcgtta ccgtcggcgg cacggaaaac ctgctgatgg
     7381 cggcgacgct ggcggaaggt acgacggttt tggaaaactg cgccattgag cctgaagtgg
     7441 tcgatttggc ggaatgcctg gtcaaaatgg gcgcgaaaat cagcggcatc ggtacgtcca
     7501 cgatggttgt ggaaggcgcg ggcgagctac acggctgcga acacagcgtc gtccccgacc
     7561 ggattgaagc ggggacgttt ctgtgcgcgg tggcgataac cggcggcagg gtggttttgc
     7621 ggaatgccgc cccgaaaacg atggaagtgg tgttggacaa actggttgag gcaggtgcgg
     7681 tgattgaagc gggcgacgat tggatcgcca tcgatatgcg gcagcgtccg aaggcggtgg
     7741 acatccgcac ggtcgtccac cccggcttcc ccacggatat gcaggcgcag tttatggcat
     7801 tgaatgccgt ggcggaggga agctgccgcg tggtggaaac gatttttgaa aaccgcttta
     7861 tgcacgtccc cgagttgaac cggatggggg cgaacatcac aaccgagggc aatacggcat
     7921 ttgtgcaggg tgtggaacag ctttccggcg cggtcgtcaa ggcgacggat ttgcgtgcgt
     7981 ccgcaagcct cgttatcgcc ggtttggcgg cgcgaggcga aaccgtggtc gaacagattt
     8041 accacttgga tcgcggttat gaaaatattg aaaaaaaact cggcagcgtc ggcgcgaaaa
     8101 tcgagcgcgt atccggctga tcgggttgcg atgccgtctg aagccttcag acg
//
LOCUS       DAWFIE010000085         8111 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWFIE010000085 DAWFIE010000000
VERSION     DAWFIE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8111
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.085"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(74..1420)
                     /gene="mnmE"
                     /locus_tag="WGQ71_001754"
     CDS             complement(74..1420)
                     /gene="mnmE"
                     /locus_tag="WGQ71_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ0707721.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGVNDKTRAILDALPSELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVVCGEITGEFTADDLLGVIFSRFCIGK"
     gene            1555..1710
                     /locus_tag="WGQ71_001755"
     CDS             1555..1710
                     /locus_tag="WGQ71_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707722.1"
                     /translation="MEKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1707..2138
                     /locus_tag="WGQ71_001756"
     CDS             1707..2138
                     /locus_tag="WGQ71_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ0707723.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2272..6636
                     /gene="app"
                     /locus_tag="WGQ71_001757"
     CDS             2272..6636
                     /gene="app"
                     /locus_tag="WGQ71_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ0707724.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGIFPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFTYKIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGRKYIDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPRQNGKYSFHD
                     NNNGTGKIDAKHEHNSLPNRLKTRTVQLFNVSLSETAQEPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDIATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKA
                     ENFQIKGGQAVVSRNVAKVKGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEK
                     TITDDKVIASLTNTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNG
                     DLSLVGNAQATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSHSALNG
                     NVSLADKAVFHFESSRFTGQISGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSATDAPRRRSRRSLLSVTPPASAESHFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAG
                     RDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDTALAKQREAETRPATTAFP
                     RARRARRDLPQPQPQPQPQPQRDLISRYTNSGLSEFSATLNSVFAVQDELDRVFAEDR
                     RNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDD
                     GIGNSARLAHGAVFGQYGIDRFDIGISAGAGFSSGNLSDGIGGKIRRRVLHYGIQARY
                     RAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHI
                     SITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAK
                     GPQLEAQHSAGIKLGYRW"
     gene            complement(6745..7467)
                     /locus_tag="WGQ71_001758"
     CDS             complement(6745..7467)
                     /locus_tag="WGQ71_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0707725.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPS
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGIDYFGTDEDIDFPDPPNMG"
     gene            7752..>8111
                     /locus_tag="WGQ71_001759"
     CDS             7752..>8111
                     /locus_tag="WGQ71_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0707726.1"
                     /translation="MSTFFQQTAQAMIAKHINRFPLLKLDQVIDWQPIEPYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSEL"
BASE COUNT         2169 a         2297 c         1926 g         1719 t
ORIGIN      
        1 aacggatcgg attcccgttt ttatgggata acggaatgtt cagccggaca ccgccacgat
       61 gctttcgatc cgtttatttt ccgatacaaa acctcgaaaa aatcacgccg agcaggtcgt
      121 ccgccgtaaa ctcgcccgtg atttcgccgc acacgacctg cgccagccgc aagtgttcgg
      181 caaacagctc gatttgatgg ttgccgcaca atgccgccag cgacaattct tcctgcgctg
      241 ctttgagtgc gttgacgtgc cgcgtccgcg ccaaaaacag cccttcgctt tcgccctgcc
      301 aaccggcctc acgcaacaac gtccgtttca gcgcgtccaa gccgtcgccc gttttcgccg
      361 acaacgcgat gacggtttcc gcgcccgtac cgaacccgcc tgccgcgtgt gcgtgcaaat
      421 cggatttgct gtggatttct atgcgtttca actccgacgg cagcgcgtcc aaaatcgccc
      481 gtgtcttgtc gttcacgcct tcgcgcggat cgaccaacac cagcgcgaca tcggcttcgg
      541 atacggcttt gcggctgcgt tcgatgccga tgcgttcgac cacgtcgtcc gtctcgcgca
      601 aacctgccgt atcgacaata tgcaccggca cgccgtcaat caggatacgt tctctgaccg
      661 catcgcgcgt cgttccggca atatcggtaa caatcgccac ttcgtcgccc gccagcgcgt
      721 tcagcaggct cgacttgccc acattcggcg cgccgaccaa tacgacattc agaccttcgc
      781 gcaaaatcgc gccctgctgc gcgttggcaa gcacatcatc cacggcgcgg cgcaagccgt
      841 ccagtttgcc gcgtgcgtct gccgcttcga gaaaatcaat gtcttcctcg ggaaaatcta
      901 acgtcgcttc gaccagcatc cgcaaggtaa tcaaatcttc gaccagaccg tgtatccgcc
      961 gcgaaaaatc gcccttgagc gaacgcaatg ccaaacgtgc cgccgaacgg ctggatgcgt
     1021 caatcaaatc cgccacgcct tccgcctgtg ccaagtccag tttgtcgttc aaaaacgcac
     1081 gtttggtaaa ctcgcctggt tcggcaaggc gcgcacccaa ttccaaacag cggttcagca
     1141 gcatatccat cactaccggc ccgccgtgtc cctgaagctc gatgacatct tcacccgtaa
     1201 aacttgccgg tgcggcaaaa aacagtagca gcccgctatc gattgcctgt ccgtccgcgt
     1261 ccgtaaaatc agcataggtt gccacgcgcg gcttgggcgt tttcccgcac aaagcctgcg
     1321 ccatcggcag caggtttttc cccgatatgc gtatcacgcc cacgccgccg cgccctggtg
     1381 cggtagcgac tgccgcaatc gttggaacgt tatccgacat aaaacccccg aaaaacaatc
     1441 aaaaacagcc gtgattatag caaatgccgt ctgaagtccg acggtttggc tttcagacgg
     1501 cataaaaccg caaaaatgct tgataaatcc gtccgcctga cctaatataa ccatatggaa
     1561 aaacgaaaca catacgccct cctgctcggt ataggctcgc tgctgggcct gttccatccc
     1621 gcaaaaaccg ccatccgccc caatcccgcc gacgatctca aaaacatcgg cggcgatttt
     1681 caacgcgcca tagagaaagc gcgcaaatga ccgaaaacgc acaggacaag gcgcggcagg
     1741 ctgtcgaaac cgtcgtcaaa tccccggagc ttgtcgagca aatcctgtcc gacgagtacg
     1801 tgcaaataat gatagctcgg cgtttccatt cgggaccgtt gccgccgccg tccgacttgg
     1861 cgcaatacaa cgacattatc agcaacgggg cagaccgcat tatggcaatg gcggaaaaag
     1921 aacaagccgt ccggcacgaa accatacggc aagaccaaac cttcaacagg cgcgggcaac
     1981 tgtacggctt catcagcgtc atcctgatac tgctttttgc cgtcttcctc gtatggagcg
     2041 gctaccccgc aaccgccgcc tcccttgccg gcggcacagt ggttgccttg gcgggtgctt
     2101 tcgtgattgg aagaagccga gaccaaggca aaaattaatg acaaatccta gggcgtgctt
     2161 catatccgcc cgaacgccga accgcacata taggcacatc ccgcgcgccg cccggaagcg
     2221 gaagccgcgc cctcccaaac aaacccgaat cccgtcagat aaggaaaaat aatgaaaaca
     2281 accgacaaac ggacaaccga aacacaccgc aaagccccga aaaccggccg catccgcttc
     2341 tcgcctgctt acttagccat atgcctgtcg ttcggcattt ttccccaagc ctgggcggga
     2401 cacacttatt tcggcatcaa ctaccaatac tatcgcgact ttgccgaaaa taaaggcaag
     2461 tttgcagtcg gggcgaaaga tattgaggtt tacaacaaaa aaggggagtt ggtcggcaaa
     2521 tcaatgacaa aagccccgat gattgatttt tctgtggtat cgcgtaacgg cgtggcggca
     2581 ttggtgggcg atcaatatat tgtgagcgtg gcacataacg gcggctataa caacgttgat
     2641 tttggtgcgg agggaagcaa tcccgatcaa catcgtttta cttataaaat tgtgaaacgg
     2701 aataattata aagcagggac taacggccat ccttatggcg gcgattatca tatgccgcgt
     2761 ttacataaat ttgtaaccga tgcagaacct gttgaaatga ccagttatat ggatgggcgg
     2821 aaatatatcg atcaaaataa ttaccctgac cgtgttcgta ttggggcagg caggcaatat
     2881 tggcgatctg atgaagatga gcccaataac cgcgaaagtt catatcatat tgcaagtgcg
     2941 tattcttggc tcgttggtgg caataccttt gcacaaaatg gatcaggtgg tggcacagtc
     3001 aacttaggta gtgaaaaaat taaacatagc ccatatggtt ttttaccaac aggaggctca
     3061 tttggcgaca gtggctcacc aatgtttatc tatgatgccc aaaagcaaaa gtggttaatt
     3121 aatggggtat tgcaaacggg caacccctat ataggaaaaa gcaatggctt ccagctggtt
     3181 cgtaaagatt ggttctatga tgaaatcttt gctggagata cccattcagt attctacgaa
     3241 ccacgtcaaa atgggaaata ctcttttcac gacaataata atggcacagg aaaaatcgac
     3301 gccaaacatg aacacaattc tctgcctaat agattaaaaa cacgaaccgt tcaattgttt
     3361 aatgtttctt tatccgagac agcacaagaa cctgtttatc atgctgcagg tggtgtcaac
     3421 agttatcgac ccagactgaa taatggagaa aatatttcct ttattgacga aggaaaaggc
     3481 gaattgatac ttaccagtaa catcaaccaa ggtgctggag gattatattt ccaaggagat
     3541 tttacggtct cgcctgaaaa caacgaaacg tggcaaggcg cgggcgttca tatcagtgaa
     3601 gacagtaccg ttacttggaa agtcaatggc gtggcaaacg accgcctgtc caaaatcggc
     3661 aaaggcacgc tgcacgttca agccaaaggg gaaaaccaag gctcgatcag cgtgggcgac
     3721 ggtaaagtta ttttagatca acaagcagat gaaaataata aaaaacaagc ctttagtgaa
     3781 atcggcttgg tcagcggcag gggtacggtg caactgaatg ccgataatca gttcaacccc
     3841 gacaaactct atttcggctt tcgcggcgga cgtttggatt tgaacgggca ttcactttcg
     3901 ttccaccgta ttcaaaatac cgatgaaggg gcgatgattg tcaaccacaa tcaagacaaa
     3961 gaatccaccg ttaccattac aggcaataaa gatattgcta caaccggcaa taacaacagc
     4021 ttggatagca aaaaagaaat tgcctacaac ggttggtttg gcgagaaaga tacgaccaaa
     4081 acgaacgggc ggctcaacct tgtttaccag cccgccgcag aagaccgcac cctgctgctt
     4141 tccggcggaa caaatttaaa cggcaacatc acgcaaacaa acggcaaact gtttttcagc
     4201 ggcagaccaa caccgcacgc ctacaatcat ttaaacgacc attggtcgca aaaagagggc
     4261 attcctcgcg gggaaatcgt gtgggacaac gactggatca accgcacatt taaagcggaa
     4321 aacttccaaa ttaaaggcgg acaggcggtg gtttcccgca atgttgccaa agtgaaaggc
     4381 gattggcatt tgagcaatca cgcccaagca gtttttggtg tcgcaccgca tcaaagccac
     4441 acaatctgta cacgttcgga ctggacgggt ctgacaaatt gtgtcgaaaa aaccattacc
     4501 gacgataaag tgattgcttc attgaccaat accgacatca gcggcaatgt cagccttgcc
     4561 gatcacgctc atttaaatct cacagggctt gccacactca acggcaatct tagtgcaaat
     4621 ggcgatacac gttatacagt cagccacaac gccacccaaa acggcgacct tagcctcgtg
     4681 ggcaatgccc aagcaacatt taatcaagcc acattaaacg gcaacacatc ggcttcgggc
     4741 aatgcttcat ttaatctaag cgaccacgcc gtacaaaacg gcagtctgac gctttccggc
     4801 aacgctaagg caaacgtaag ccattccgca ctcaacggta atgtctccct agccgataag
     4861 gcagtattcc attttgaaag cagccgcttt accggacaaa tcagcggcag caaggatacg
     4921 gcattacact taaaagacag cgaatggacg ctgccgtcag gcacggaatt aggcaattta
     4981 aaccttgaca acgccaccat tacactcaat tccgcctatc gccacgatgc ggcaggggcg
     5041 caaaccggca gtgcgacaga tgcgccgcgc cgccgttcgc gccgttccct attatccgtt
     5101 acacctccgg cttcggcaga atcccatttc aacacgctga cggtaaacgg caaattgaac
     5161 ggtcagggaa cattccgctt tatgtcggaa ctcttcggct accgcagcga caaattgaag
     5221 ctggcggaaa gttccgaagg cacttacacc ttggctgtca acaataccgg caacgaacct
     5281 gcaagcctcg aacaattgac ggtagtggaa ggaaaagaca acaaaccgct gtccgaaaac
     5341 cttaatttta ccctgcaaaa cgaacacgtc gatgccggcg cgtggcgtta ccaactcatc
     5401 cgcaaagacg gcgagttccg cctgcataat ccggtcaaag aacaagagct ttccgacaaa
     5461 ctcggcaagg cagaagccaa aaaacaggcg gaaaaagaca acgcgcaaag ccttgacgcg
     5521 ctgattgcgg ccgggcgcga tgccgtcgaa aagacagaaa gcgttgccga accggcccgg
     5581 caggcaggcg gggaaaatgt cggcattatg caggcggagg aagagaaaaa acgggtgcag
     5641 gcggataaag acaccgcctt ggcgaaacag cgcgaagcgg aaacccggcc ggctaccacc
     5701 gctttccccc gcgcccgccg cgcccgccgg gatttgccgc aaccgcagcc ccaaccgcag
     5761 ccccaaccgc agcgcgacct gatcagccgt tataccaata gcggtttgag tgaattttcc
     5821 gccacgctca acagcgtttt cgccgtacag gacgaattag accgcgtatt tgccgaagac
     5881 cgccgcaacg ccgtttggac aagcggcatc cgggacacca aacactaccg ttcgcaagat
     5941 ttccgcgcct accgccaaca aaccgacctg cgccaaatcg gtatgcagaa aaacctcggc
     6001 agcgggcgcg tcggcatcct gttttcgcac aaccggaccg aaaacacctt cgacgacggc
     6061 atcggcaact cggcacggct tgcccacggc gccgttttcg ggcaatacgg catcgacagg
     6121 ttcgacatcg gcatcagcgc gggcgcgggt tttagcagcg gcaacctttc agacggcatc
     6181 ggaggcaaaa tccgccgccg cgtgctgcat tacggcattc aggcacgata ccgcgccggt
     6241 ttcggcggat tcggcatcga accgcacatc ggcgcaacgc gctatttcgt ccaaaaagcg
     6301 gattaccgct acgaaaacgt caatatcgcc acccccggcc ttgcgttcaa ccgctaccgc
     6361 gcgggcatta aggcagatta ttcattcaaa ccggcgcaac acatttccat cacgccttat
     6421 ttgagcctgt cctataccga tgccgcttcg ggcaaagtcc gaacgcgcgt caataccgcc
     6481 gtattggctc aggatttcgg caaaacccgc agtgcggaat ggggcgtaaa cgccgaaatc
     6541 aaaggtttca cgctgtccct ccacgctgcc gccgccaaag gcccgcaact ggaagcgcaa
     6601 cacagcgcgg gcatcaaatt aggctaccgc tggtaacacc ggatatgccg aaaggggttt
     6661 gacgatgtcg ccgtgcgctg tcaaaccctt tttctgccgc cgccttgtgc ctgctccatt
     6721 gtctgataac cgtcaaattg atttttagcc catgtttggt gggtcgggga aatctatatc
     6781 ttcgtccgtg ccgaaatagt ctatgcccga tatacaattt tgatacacaa acttggaaat
     6841 atcggtatcg tcgccggagc gatagaatgc ggacagtttt tcattgaatt gcggcatctg
     6901 catttcggag atttccaaga tgccgcagcc ccccgccatc atcagaccgt tggcaaataa
     6961 ggttgccgtt cgtttgttgc cgtcccaaaa aacctgctgc cgcatacaat aaagcataaa
     7021 acggacggct gcctccgtcg tcgaaccgct ttgcagtccg atattttgta acacgcgagc
     7081 cacttcaatt tccttcactg gattcggggc atgacgggaa ccgtccaata gcgttacgcc
     7141 gaccgaaccg gtacggaaat cactgggtgc caaagaatcg tccttggcaa caatgttgtt
     7201 gattttttta aggagtgaaa tatcgaccgg ttcgccgttt gaaatatgcg aaatcacata
     7261 ttgataggca cgtttcaggt tcaagatggt ttggatgtct tccagtgacg cggaggctac
     7321 atttttgcca tggataattt gttcggtctg caacaaggtc gtctgacaat tctcaaaacg
     7381 gctgaggttg tgaatttggg caaccaatac tttctttgcc aaaaaaatat tttctttcaa
     7441 actcaacttg tatttgtcgg gaaacacgat aattccttaa ataattaaag cagtacgtta
     7501 aatcatcatc ttatcatcac tttcggggtt tatgttgcgt gcgtccgttt tctaatacgg
     7561 tttcccatcc cgcgaaacga ggcggagacc tgtgcaaaaa acctgttcac gaaattttcc
     7621 gcattaacga tacgctgaaa atgcgctaaa aatgactgtg tttgaatatc ggttgatttc
     7681 atccgtttga gacctttgca aaattcccca aaatccccta aattcccacc aagacattta
     7741 ggggattttc catgagcacc ttcttccagc aaaccgccca agccatgatt gccaaacaca
     7801 tcaaccgttt cccgctattg aagttggacc aagtgattga ttggcagccg atcgaaccat
     7861 acctgaaccg tcaaaaaacc cgttacctcc gagaccaccg cggtcgtccc gcctatcccc
     7921 tgttgtccat gttcaaagcc gtcctgctcg gacaatggca cagcctctcc gatcccgaac
     7981 tcgaacacag cctcatcacc cgcatcgatt tcaacctgtt ttgccgtttt gacgaactaa
     8041 gcatccccga ttacagcacc ttatgccgct accgcaattg gctggcgcaa gacgacaccc
     8101 tgtccgaact g
//
LOCUS       DAWFIE010000086         8037 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWFIE010000086 DAWFIE010000000
VERSION     DAWFIE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.086"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            222..320
                     /locus_tag="WGQ71_001760"
     CDS             222..320
                     /locus_tag="WGQ71_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ0707727.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(391..1776)
                     /locus_tag="WGQ71_001761"
     CDS             complement(391..1776)
                     /locus_tag="WGQ71_001761"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ0707728.1"
                     /translation="MVSIFDIFKIGIGPSSSHTVGPMKAAAAFATGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNGLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(1875..4265)
                     /gene="gyrB"
                     /locus_tag="WGQ71_001762"
     CDS             complement(1875..4265)
                     /gene="gyrB"
                     /locus_tag="WGQ71_001762"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ0707729.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINNYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            4535..5407
                     /locus_tag="WGQ71_001763"
     CDS             4535..5407
                     /locus_tag="WGQ71_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0707730.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            5495..7531
                     /locus_tag="WGQ71_001764"
     CDS             5495..7531
                     /locus_tag="WGQ71_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ0707731.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELLDGGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSTHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
BASE COUNT         1729 a         2256 c         2199 g         1853 t
ORIGIN      
        1 catattttag aaaagacctt ttatagtgga ttaacaaaaa ccagtacagc gttgcctcgc
       61 cttagctcaa agagaacgat tctctaaggt actgaagcac caagtgaatc ggttccgtac
      121 tatttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actataaaaa
      181 ttcttgccgg atgctgcaaa caacgccggt ttgcattcct gatggcggtg gttttcttag
      241 acgaacgccc gaacacgcag gaatggatag gcttggggct ggttacggcg ggcgtgttga
      301 cgctggcact gaaacggtaa agccgcaaga aataaatgaa atgccgtcta aaaaactgtt
      361 ttcagacggc attttcgttt ctgtccatcc tcagcactcg accacgcgca cggatacggg
      421 gacggctttt ttccggagcg tggcggcgag tccggcaaga gaggtttctt tgtaggtcgc
      481 cttcatatcc cggccggttt gcagcatggt tcggatgact tcgtcgagcg agactttttt
      541 gtccgtgccg tcttccaaaa gcgcgagcgt gccgagtttg agggcttttt cggcggcgat
      601 gccgttgcgc tcgatgcagg ggatttgcac cagtccgccg acggggtcgc aagtcagccc
      661 caaatggtgt tccatcgcca tttcggcggc gttttccact tgtttgggcg tgccgccgat
      721 gacttcggcg tatgcgcccg ccgccatcga acacgctacg ccgacttcgc cctgacagcc
      781 gacatccgca ccggaaatgg aggcgttggt cttgtagagg atgccgattg cgcctgcggt
      841 gagcaggaag ttttcgacgc gttcctgtgt ggcgtgcgga ttgaacttgc ggaaatagtg
      901 caatacggcg ggaatgatgc ctgccgcgcc gttggtcggt gcggtaacga cgcgtccgcc
      961 ggcggcgttt tcttcgttga ccgccatggc gtacaccatc ggccagagct gggtgttgac
     1021 gatttcggtt tcgcgcagga ctttgagctt ggcggcaagc tgcggggcgc ggcggcggac
     1081 gttcaatccg ccgggcagtt cgccgtccgc acccaagccg cgtttgatgc agccttccat
     1141 aacctcggca acggcagcgg cgcggcggcg gatttcggct tcgccgcatc cggcaagcgc
     1201 ggcttcgttt gccaacacga cttcggagat gtcgagccgg ttcagacggc atcgggcaag
     1261 cagttcggcg caactggtat agggataggg aacggctttt tccgtttccg cctgccggtc
     1321 aaaatcttct tcggtaacga caaagccgcc gccgaccgaa taataaacct gttcattcaa
     1381 taccgtgccg tctgaagcat aggcggtaaa acgcaggccg ttggggtgtt tgggcagcac
     1441 ttgattgccg agtatgttca ggtcgcggtc ggggatgaag cggatttctt gcccgttgag
     1501 ccggaggatg tgctgcgtgc ggatgcgttc gaggcgttcg ggaatgccgg caagcgggat
     1561 gtcgtgcggc aggctgcctt ccaaaccgag catcagcgcg tcaaatgtac cgtgtccgta
     1621 tccggtcagt gcgagcgagc cgtaaatgtc gatgacgatg cgaacagcct gtgcatccaa
     1681 acctgtcgca aaggcggcgg ctgccttcat cgggccgacc gtatgcgaac tggaaggccc
     1741 gataccgatt ttgaaaatat caaaaatgct gaccatggtt ttgctccgaa aggcggtttc
     1801 agacggtatg gcttccgttt gacaaaccaa aaggagacgc ggcacgatgc ccgtctcctt
     1861 ttttaaaacg gcacttatgc gtcgatattt tgggcaatca gcgcgttgtt ttcgataaag
     1921 gcacggcgcg gctcgacctc gtcgcccatc agcgtaacga acacttcgtc ggcggcaatg
     1981 gcatcttcga tgcgcacttt caacaggcgg cgcacggcgg gatccatcgt ggtttcccac
     2041 agctgctcgg ggttcatctc gcccaagcct ttgtatcgtt ggatggacat acctttttgg
     2101 gcaacgctca tcaagatgtc caaagcggtt tcaaagctgt ccgcgtcgta cccgttttcg
     2161 cctttgtaaa gcttggcacc ctcgccgacc atgcctttga gcgcggcggc ggtttgggtg
     2221 agggtttggt aggctttgct gttgaggaac ttgggttcga tgtagctgac catgacgttg
     2281 ccgtgcagct tgcgcgtgat tttgatgaac cggtgtcctt catgaccttc gatgcgttcg
     2341 agggcgactt ctttttcgtc aagcagaccg gaaagttcgg caacggcttt atcggcgttt
     2401 tcagacgacg tcaaatcaat gggcgacgcg tgtagcatgg cgcgcaggac gagttcgtct
     2461 acgaagcggc tttcctgttc gatgacggtt tttgccaaca ggaattgttt ggcggtgtcg
     2521 gcaagttctg cgccttcgat ggtgcggccg tctgaaatga ttttggcttt ttccaaggca
     2581 agaccgagca gccattggtc tttttccaat tcgtctttga gatatcgttc ttgtttgccg
     2641 tatttggctt tgtataaagg cggctgggca atgtagatgt agccgcgttc gaccagctcg
     2701 ggcatttggc ggtagaagaa ggtcaggagc agggtgcgga tgtgcgcgcc gtccacgtcg
     2761 gcatcggtca tgatgatgat gcggtggtaa cgcagttttt cggcattgaa ttcttctttg
     2821 ccgatgcccg cgcccaacgc ggtaatcaaa gcggctactt cctgactggc gagcattttt
     2881 tcaaagcgcg ctttttcgac gttcaaaatc ttgcccttaa gcggcaaaat cgcttggaat
     2941 ttgcggtcgc gcccctgcat ggcggaaccg cctgcagaat cgccctcgac cagataaagt
     3001 tctgacaatg cggggtcttt ttcttggcag tcggcgagtt tgccgggtag ccccaagccg
     3061 tccatcacgc ctttgcggcg ggtgatttcg cgtgctttgc gggcggcttc gcgcgcgcgg
     3121 gcggcatcga cgattttgcc ggtgatgatt ttggcttcgg cagggttttc ttcgaggaag
     3181 tcggtcaggg cttggctgat gacttcgttg acgacggggc cgatttcgcc ggaaaccagt
     3241 ttgtctttgg tttgcgatga gaatttcgga tcgggcagtt tgacggacaa aacgcaggtc
     3301 agaccttcac gcatatcgtc gccggcggtt tccactttgg cttttttagc cacttcgttg
     3361 gcttcgatat agttgttgat ggtgcgggtc atcacttggc gcaacgcggt caagtgggtg
     3421 ccgccgtcgc gttgcggaat gttgttggta aaacactgca cgctttcttg gtagctgtca
     3481 ttccactgca tcgcgcattc gacgctcatg ccgtctttct cgccgaacgc gtagaagatt
     3541 ttttcgtgca agggcgtttt tttgcggttc atgtattgca cgaatccagc cacgccgccg
     3601 gaaagggcga agctttcgtg tttgccgtcg cgctcgtcgg tcaattcgat gtccacgccg
     3661 ttgttcagga acgaaagttc gcggatgcgt ttggcaagga tgtcgaagct gtattcgacg
     3721 ttgccgaagg tttccgtact ggcgaggaag cgcacggtcg tgcctttttt atcggaatcg
     3781 ccgaccactt tcaacggctc ttcggtttcg ccgcgcacga agcggacgaa gtgttctttg
     3841 ccgtcgcggt agatggtcag cgtaacccag tcggacagcg cgttgacgac ggacacgccc
     3901 acgccgtgca ggccgccgga gattttgtag ctgttgttgt cgaatttacc gcccgcgtgc
     3961 aatacggtca tgatgacttc ggcggcggag cgtccttctt tcgggtggat gccggtgggc
     4021 ataccgcgcc cgttgtcggc gacgctgacg gaatggtcgg cgtgtatcgt taccgtgatt
     4081 ttgtcgcaat gtccggcgag tgcttcgtca atggcgttgt ccaatacttc gaacaccatg
     4141 tggtgcagac cgctgccgtc ctgcgtgtcg ccgatgtaca tgccggggcg tttgcgtacc
     4201 gcttccaagc cttcgagcac ctgaatgctg tcggcgccgt attcttcgtg tttttgttca
     4261 gtcatatttt ttgccggatt ttgaaaagat tttgcgatgc cgccaaaaca agtccgcacc
     4321 ttgtagaaaa agcgggcggg acgacatata taattgtgta ttatagccga ttttgccgcc
     4381 taattcagcg ttatccgcat cagtgtgccg ccgggaaaag atgaaacggt acgtttgcct
     4441 ccggcatcag gtcggggatt gtcccgtaaa gtggcaaaag cgtttttttg ccactaaaat
     4501 ctacacccta tacttttcgg acaggggcgc ggaaatggaa atatggaata tgttggacac
     4561 ttggctcggt gccgtcccga tacgtgcgga ggcggtcgaa tccgtggcgg cggttgcggc
     4621 tttgctgctg gcgcgcgccc ttctgttgaa tatccacttc aaacggcatc cggatttcgg
     4681 catcgaaagc aagcggcggt ttttggttgc cagccgcaat ataacgctgc ttttggtgct
     4741 gttttcgctg gcatttatct ggtcggcgca aatccaaacg ctggctttgt cgatgtttgc
     4801 ggtggcggcg gcggtcgtcg tggcgacgaa ggaactgatt atgtgtctgt cgggcagtat
     4861 tttaaggtct gccacccagc aatactcggt cggcgactat atcgaaatca acggcctgcg
     4921 cgggcgcgtg gtcgacatca acctgttgaa cacgctgatg atgcaggtcg gtccgaaccc
     4981 cttggtcgga cagcttgcgg gaaccaccgt ttctttcccc aacagcctgt tgttgagcca
     5041 ccccgtgcgc cgcgacaata ttttgggcga ctatgtcatc catacggtcg aaatccccgt
     5101 tcccatccat ttggattcgg atgaagccgt atgccgtctg aaagccgtac tcgagccctt
     5161 gtgcgcgccc tacatccccg ccatccaacg gcatttggaa aacgtgcagg cggaaaaact
     5221 gtttatcacg cccgccgcca gaccgcgcgt tacccgcgtg ccgtacgatg acaaggcata
     5281 ccgcatcatc gtccgcttcg cctcccccgt ttcaaagcgg ctggaaatcc aacaggcggt
     5341 tatggacgaa tttttgcgcg tacaataccg cctgttaaat taccccgccg gctccgaaac
     5401 actttaactt tccccgaccg accccatttc cggcttcaga cggcatattg ccgatatgct
     5461 gtctgaagca caacacgcaa aggaaaccca tcttatgact gacaacgcac tgctccattt
     5521 gggcgaagaa ccccgttttg atcaaatcaa aaccgaagac atcaaacccg ccctgcaaac
     5581 cgccatcgct gaagcgcgcg aacaaatcgc cgccatcaaa gcccaaacgc acaccggctg
     5641 ggcaaacact gtcgaacccc tgaccggcat caccgaacgc gtcggcagga tttggggcgt
     5701 ggtgtcgcac ctcaactccg tcgccgacac gcccgaactg cgcgccgtct ataacgaact
     5761 gatgcccgaa atcaccgtct tcttcaccga aatcggacaa gacatcgagc tgtacaaccg
     5821 cttcaaaacc atcaaaaact cccccgagtt cgacaccctc tcccccgcgc aaaaaaccaa
     5881 actcaaccac gatctgcgcg atttcgtcct cagcggcgcg gaactgccgc ccgaacagca
     5941 ggcagaactg gcaaaactgc aaaccgaagg cgcgcaactt tccgccaaat tctcccaaaa
     6001 cgtcctagac gcgaccgacg cgttcggcat ttactttgac gatgccgcac cgcttgccgg
     6061 cattcccgaa gacgcgctcg ccatgtttgc cgctgccgcg caaagcgaag gcaaaacagg
     6121 ctacaaaatc ggtttgcaga ttccgcacta cctcgccgtc atccaatacg ccgacaaccg
     6181 cgaactgcgc gaacaaatct accgcgccta cgttacccgc gccagcgaac ttttagacgg
     6241 cggcaaattc gacaacaccg ccaacatcga ccgcacgctc gcaaacgccc tgcaaaccgc
     6301 caaactgctc ggcttcaaaa actacgccga attgtcgctg gcaaccaaaa tggcggacac
     6361 ccccgaacaa gttttaaact tcctgcacga cctcgcccgc cgcgccaaac cctacgccga
     6421 aaaagacctc gccgaagtca aagccttcgc ccgcgaaagc ctgaacctcg ccgatttgca
     6481 accgtgggac ttgggctacg ccagcgaaaa actgcgcgaa gccaaatacg cgttcagcga
     6541 aaccgaagtc aaaaaatact tccccgtcgg caaagtatta aacggactgt tcgcccaaat
     6601 caaaaaactc tacggcatcg gatttaccga aaaaaccgtc cccgtctggc acaaagacgt
     6661 gcgctatttt gaattgcaac aaaacggcga aaccataggc ggcgtctata tggacctcta
     6721 cgcccgcgaa ggcaaacgcg gcggcgcgtg gatgaacgac tacaaaggtc gccgccgttt
     6781 ttcagacggc acgctgcaac tgcccaccgc ctacctcgtc tgcaacttcg ccccacccgt
     6841 cggcggcagg gaagcccgcc tgagccatga cgaaatcctc accctcttcc acgaaaccgg
     6901 ccacggcctg caccacctgc ttacccaagt ggacgaactg ggcgtgtccg gtatcaacgg
     6961 cgtagaatgg gacgcggtcg aactgcccag ccagtttatg gaaaatttcg tttgggaata
     7021 caatgtattg gcgcaaatgt ccacccacga agaaaccggc gagcccctgc cgaaagaact
     7081 cttcgacaaa atgctcgccg ccaaaaactt ccaacgcgga atgttcctcg tccgccagat
     7141 ggagttcgcc ctcttcgata tgatgattta cagcgaagac gacgaaggcc gtctgaaaaa
     7201 ctggcaacag gttttagaca gcgtgcgcaa agaagtcgcc gtcatccaac cgcccgaata
     7261 caaccgcttc gccttgagct tcggccacat cttcgcaggc ggctattccg caggctatta
     7321 cagctacgcg tgggcggaag tattgagcgc ggacgcatac gccgcctttg aagaaagcga
     7381 cgatgtcgcc gccacaggca aacgcttttg gcaggaaatc ctcgccgtcg gcggatcgcg
     7441 cagcgcggca gaatccttca aagccttccg cggccgcgaa ccgagcatag acgcactctt
     7501 gcgccacagc ggtttcgaca acgcggcttg atcgatgtga acaaaaaatg ccgtctgaaa
     7561 caatcaaaca gtgtttcaga cggcatcagc atttgcgcgg tttcgccatt ttcacaaaaa
     7621 cagtaagcca aaatcccgtc attcccgcgt aggcgggaat ccagaccggt cggcacggaa
     7681 acttatcgga taaaacggtt tctttagatt ttacgttcta gattcccgct ttcgcgggaa
     7741 tgacgcggaa aagttgctgt gatttcggat aaattttcgc aacgtttaat ttccgtttta
     7801 cctgataaat gccctcaatc tcaaatcccg tcattcccca aaaacaaaaa aaatcaaaaa
     7861 cagaaatccc gtcattcccg cgcaggcggg aatccaggtc tgtcggtgcg gaaacttatc
     7921 ggataaaacg gtttctttag attttacgtt ctagattccc gctttcgcgg gaatgacgga
     7981 atatttttga atttgataaa aatgccgtct gaaacggtca aacaacgctt cagacgg
//
LOCUS       DAWFIE010000087         7990 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWFIE010000087 DAWFIE010000000
VERSION     DAWFIE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.087"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(159..1859)
                     /gene="recJ"
                     /locus_tag="WGQ71_001765"
     CDS             complement(159..1859)
                     /gene="recJ"
                     /locus_tag="WGQ71_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ0707732.1"
                     /translation="MSVKIQTRSINTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAAGRLADAVERQEKILIVADYDADGATACSVGMSGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFQSKSLAGVGVIFYVLTALRAELRRRDYFSDGIKEP
                     NLGDLLDLVALGTVADVVTLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIDCLLARDDVEAQELAAQLNNLNIERREIEQSM
                     LQDALSDFPKTLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFFRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDVLDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTYITDGSLKACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGYEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAV
                     "
     gene            complement(1928..2644)
                     /locus_tag="WGQ71_001766"
     CDS             complement(1928..2644)
                     /locus_tag="WGQ71_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707733.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTLMLWGMNNPVSETITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTDGLCLRYDTDRPDDIAKLKQLEFEAVELDKQTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPVEIYYMVKGEYTDKLKLFGNILYTPPLLILDAA
                     GAVLALPTAALGAVVDAARK"
     gene            complement(2691..3197)
                     /locus_tag="WGQ71_001767"
     CDS             complement(2691..3197)
                     /locus_tag="WGQ71_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ0707734.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RTRMELLADDGALTQFMSDFPHADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MENGDAEI"
     gene            3352..4683
                     /gene="pmbA"
                     /locus_tag="WGQ71_001768"
     CDS             3352..4683
                     /gene="pmbA"
                     /locus_tag="WGQ71_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ0707735.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGFAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(4980..5183)
                     /locus_tag="WGQ71_001769"
     CDS             complement(4980..5183)
                     /locus_tag="WGQ71_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ0707736.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            5426..5728
                     /gene="clpS"
                     /locus_tag="WGQ71_001770"
     CDS             5426..5728
                     /gene="clpS"
                     /locus_tag="WGQ71_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ0707737.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            5730..>7990
                     /gene="clpA"
                     /locus_tag="WGQ71_001771"
     CDS             5730..>7990
                     /gene="clpA"
                     /locus_tag="WGQ71_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ0707738.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDDEDGNDSDGLGREG
                     EEAEQKTGSLSDYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLVGEAG
                     VGKTALAEGLAHQIVNSDIPDALKDTEVYALDMGSLLAGTKYRGDFEARVKSVLKQLE
                     KIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRTIFDK
                     DHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSARYINE
                     RFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVSRIPEKTVSHDDKQVL
                     QFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGKTEAAKQ
                     LAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVLLLDE
                     IEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGFTAKR
                     ERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLDKKVEAE
                     FTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRIDWDTAK
                     EEAVLKFKKSKVK"
BASE COUNT         1910 a         2343 c         2030 g         1707 t
ORIGIN      
        1 agtctgaaat ttgatgcacc ttatatattg cgtccctaag aaaggatgat taacaaaaat
       61 taacgccctt tactttcttt tccgattggg aacattcccg tcgtactaac aggcaaatga
      121 aggaacagaa atcacattca aacggcgttc cgccgcctct acaccgcttc ccagtagtcg
      181 atataaagct gcaattcgag attattccgc cattcgttgg caacggggcg gtacaccgtg
      241 cggatgtatt cgggaatgtc ttcgctacaa cgccaaaaca tggcttcaaa ttcgtagccg
      301 tctttttgca gccagacttt tttatgtttg ccttccgcac ccaaaggctg ctggcggaca
      361 acgtgaaatt cgtcggtaaa actcggcggc gcgaagccct gcccccaaac gtggcgggcg
      421 aggttttgcg cctgttccaa cgtgatgtcg caggctttca ggctgccgtc ggtaatatag
      481 gtctgcgaca aatcgtcttc gcacaccatc tcgcgcacgg cttcttcaaa ggctgtctga
      541 aacgcgggaa tattgtgttc gagtatgctc aaacccgccg ccattgcgtg tccgccgaat
      601 ttcaaaatca aatcgggatg gcgtttggac accaagtcca aaacatcgcg caggtgcaaa
      661 ttgggaatgg agcgtcccga accacgcact tcgccgttgt cggcaggcgc aaacacgatg
      721 gtcggacgaa aaaaacggtc tttgagacga ctggcaacga taccgaccac gccttgatgg
      781 aagtcgtcgc gatacgccac caaagtcgtc tgacctgaag gcagggtttt gggaaaatca
      841 ctcagcgcgt cttgcagcat ggattgctcg atttcgcggc gttcgatatt gaggttgttt
      901 aactgggccg ccagttcctg cgcttccaca tcatctcgcg ccaacagaca gtcaatgccg
      961 accgacatat cgtccagccg tccggcggcg ttgatgcgcg gtcccaacgc aaagcccata
     1021 tcaaacggct gagccttgcg ccaatcccgt cgtgctactt caaacaaggc gcggatgccg
     1081 gggcgcattt tgcccgaacg catccgtttc aaaccttgcg acacgaggat acggttgttg
     1141 tggtctagag taacgacatc ggcgacagtg ccgagtgcga ccaaatccaa aaggtcgccc
     1201 agattcggct ctttgatgcc gtctgaaaaa taatcgcggc ggcgcagttc ggcacgcaac
     1261 gccgtcaata cataaaaaat cacgcccacg cccgccaaac ttttgctttg gaagccgcag
     1321 cctttttggt tcggattgac gatgatgcag tcgggcacgg tctcggcagg cagatggtgg
     1381 tcggtaacga tgacatccaa acccagagcc tgcgcgcgcg ccacgcctgc gatgctggca
     1441 atgccgttat cgaccgtaat cagcaaatct acaccttgct ctgccgcgat ttcagccagc
     1501 tcgggcgtta aaccgtagcc gtgttcaaag cggttgggca caaggaaatc cactttcgcc
     1561 cccatcgccg ccaaaccgct cataccgacg gaacacgccg tcgcgccgtc ggcatcatag
     1621 tcggcgacaa tcaggatttt ttcctgacgc tcaaccgcat ccgccaaacg gccggcggcg
     1681 gcttcgcaat tcgtcagcga ttgataaggc aggagggaag cgagtttgtc gtccaattcg
     1741 gcaggacttt gcacaccgcg cgaagcacaa agccgggcga ttaaaggatc ggcaccggcg
     1801 gtgagcaaat gattaaaaac gtcggtattg atggatcggg tttggatttt gactgacata
     1861 acgtggggtt ttactgtgcg tgtgtgctta aaatgccgtc tgaaagcttt tcagacggca
     1921 ttgctgttca tttgcgggcg gcatccacga ccgcacccag agccgccgta ggcaaggcca
     1981 gtaccgcgcc cgccgcatcc agtatcaaca aggggggcgt atataagata tttccaaaca
     2041 gcttgagttt gtcggtatat tctcccttaa ccatataata aatctcgaca ggcacacttt
     2101 gctcaaaatg gtaatcggcg ttcagttttt gcggtgtggc gtagtatttg cctttggcag
     2161 agacgcagcg cgtgtaaata gtctgtttgt cgagttcgac cgcttcaaac tcaagctgtt
     2221 tcagcttggc gatgtcgtca ggcctgtcgg tatcgtagcg caggcaaagg ccgtcggtac
     2281 tgaaattctg gctgccgggc gattcgagtt tgaccggcag ggcttggtgg cgggcatagc
     2341 tcggggtatc ctcaactatt tggaagggct tgtccagccc ggccttcaaa atgcccgtca
     2401 gcttcgccga atcttcagga ttgacgacga accagtattt tccgcccatc atcaccaggc
     2461 tgcccttttc caattgggca ttgtcttcgg caaccacacc gaaggcgcgg atttggtctt
     2521 tgtcaacgtg tttgcgggtg attgtttcgc tgaccgggtt gttcattccc cacaacatca
     2581 acgtacagcc gttcagcatc aaggttgccg ccaaaacggc ggcggtcgtt ttacggaaca
     2641 acattttatc ctccaatcta aataacggcg gatgtcgtct gaaaatatgc ctaaatttcc
     2701 gcgtccccgt tttccatcac ggttttcaac tcttgaaaca gggcgcggaa attttttggt
     2761 ggtttgtttt gctcctgctc ttttttggta ttgcggatga gtgtcctcag cttgcccgcg
     2821 tccgcatgcg gaaaatccga cataaactgc gtcaacgcgc cgtcgtctgc cagcagctcc
     2881 atccgcgtcc gctccacacg ttgcaaaaag gcgttgtgcg ccgcatcgtc gccgcgcagc
     2941 ttggcaagaa atgcctcgat gggcgcggga tcggtatcgc gcatcagtct gccgataaat
     3001 tgcgcctgcc gcttgagcgc gccgttggat gtgatttttt tataggtaac gacggcttcg
     3061 tacaaatctt cgtccaagcc gatttttttc aacgtgtcgc ttgagagctt ggtcagctcc
     3121 atacccaaat cctgcaaacc gttcatctgc tttttcattt gggttttgct gacccattcg
     3181 tcttcttgtt caaacatctt cattctcaaa ttttatccga cgggcgcatt ttaacagaat
     3241 gattccgcgt tcagacggca ttttgcaccg tccgccctgc aaacggcacg cttttgcccc
     3301 tgccgcccga aacggttaaa atgccgcaac gctccaccaa gaaagatttc tatgctgttc
     3361 aaccacaccg cttccgaact tctcgacctc tgccgccgca cgctcgactt ggcaaaagca
     3421 gcgggcgcga ccgccgccga agccgatttc agcgaatcca tcggacaaag cgtcagcgtg
     3481 cggctgggcg aaatcgaaca aatcgagttc cagcaggaca agtcgctgga cattaccgtt
     3541 tacgtcggca aacgcaaagg ccgcgctggc accgccgatt tctccgaaca agccctgcgc
     3601 gacaccgtcc gcgccgccgc cgacatcgcc cgccacaccg ccgaagacgg ttgcgccgga
     3661 cttgccgacc ccgagttgat ggcgcaacac atcggcgacc ccgaccttta ccacgaatgg
     3721 gatttggata cggaagccgc cgtcggcttt gcaaaacaat gcgaacaagc cgccctgaac
     3781 gaggatgagc gcatcgaaaa ctccgaaggc gcggcggtgc aaaccggcca ttaccaatac
     3841 gtttacggca acacccacgg ttttgccgca caccggcaag gcacgcacca cagcatttcc
     3901 tgcagcgtcg ttgccgccga cgaaaacggg atgcagcgcg actactggta cgattccgcc
     3961 tgccgccatc cggatatgga cagccccgaa accatcggtc aaaccgccgc ccgccgcact
     4021 ttgcgccgac tcggcagccg cagcatcccg acaggcagct accccgtcct cttcgatacc
     4081 accgtttcgg gcggtcttat cggacacctc gtcggcgcac tctccggcgg cgcgctctac
     4141 cgccaaagca gtttcctgat cgacagcatc ggcaaaaaag tcctgcccga tttcctcaac
     4201 ctgcgcgaag aaccgcacat cccccgctct ttccgcagca gctattttga tgcggaaggc
     4261 gttgccaccg cgccgcgctt cgtgattcaa aacggcattg tcgaaggcta tttcctcagc
     4321 agttacagcg cgcgcaaact cggtatgcag accacgggca acgccggcgg cgcgcacaac
     4381 ctgtatttga accatacgca cgaaacgcaa tccgacctgc tgaaagaaat ggggacggga
     4441 ttgttggtaa ccgaactgat gggacagggc gcgaacacca ttaccggtga ctactcgcgc
     4501 ggcgcggcgg gtttttgggt ggaaaacggc gtgattgcct accccgtcca cgagattacc
     4561 gtagccggac gcttgcagga tatgtaccgc gacattgtcg gcgtggcgga tgacgctttg
     4621 cggcgttcgt ccaacaaaat cggctcgatt ctgattgcgg ggatgacggt tgccggaagc
     4681 tgaacgccct gccgatgccg tctgaagacg aaggcagcac gttttgcccc gtgcctgccc
     4741 gtttgccgtt ttatgccgca ccgccttttg ccgacaacat tgcacatttt ttcatcagca
     4801 ctgattttcc cttatccgtc aaaatactaa gccacttgtc cacaacaggg tacacacaag
     4861 ttcaggataa ggcgtgcggg gattggggtt tttccgccgc cggcaaaacg gcagacaaaa
     4921 acggcgggac attgcctgtc ccgccgtttt caaatataca tttgaccgta catcaggaat
     4981 taagcagcct gaatgttggc ggcctgtttg cctttagggc cggtggttac gtcgaaagag
     5041 acacgttggc cttctttcag ggttttgaaa ccttccatat tgattgctga gaagtgagcg
     5101 aacaaatctt cgccgccttc atcaggcgtg atgaaaccaa aacctttagc gtcgttaaac
     5161 cattttacga taccggttgc cattagaaac ttcctatact caaaaattaa caaaatcagc
     5221 aaaacaaggc atacggcaaa ccgagccgtc tggcgttcct tccagcttga tatactcggc
     5281 ggatgcccaa tcctgcttaa ccgtcttttt acatttgtta agacaaaacg tcaagctatg
     5341 ttttcaaaaa agtgggaaaa taggcaaaag taccgaaaat acaacaaaaa tgccgtctga
     5401 acgcggcttt caaatgggac acactatgac cgcgcaacac caatccgaca cgctcttgca
     5461 ccgtctgaac accctgccgc cgaaacgtta cggcgttttc ctattgaacg acgattacac
     5521 cacgatggaa tttgtcgttg aaatcctgac cgaaatcttc atgctcggac aagaacaggc
     5581 ggtagcggta atgctcttgg ttcatcacga aggcaaaggc ctgtgcggca cttacacgcg
     5641 cgatattgcc caaaccaaac agcaacaagt catgcagcgg gcaaaagccg aagggcatcc
     5701 gctgcaatgt attgtcgagg agatttaata tgcttgcacc cgaattggaa cagattttgc
     5761 agcagcttta ccgcgaggcg cgtaaggctc attatgaatt tatcagcctc gagcatctgc
     5821 ttttggtact catcgaagaa gatgcctccg tgccgaacgt cttaaaacta tgcggcgcgg
     5881 atttgaaagt ggtgtccgaa cagctcgccg ccagcgttgc cgaaaacacc cccctgattc
     5941 ccgaacacct tttagacacg gtcgaaacac ggcccacgct cggcttccaa cgcgtgatgc
     6001 aacgggcgat ggtgcatacc cagtctgccg gaaaagccgc agtcgaaccg ttggacgttt
     6061 tggtcgcgct gatgagcgaa accgacagcc acaccgtcta tttcctcaag ctgcaatcgg
     6121 ttacgcgttt tgaagttttg cgctgtattg cccacggctc gcccgatgac gaggatggca
     6181 atgattcaga cggcctggga cgcgagggcg aagaagcgga acaaaaaacc ggctcccttt
     6241 ccgactacac cgtcaacctc aacgccgaag tcaaagccgg ccgtatcgac cctttgattg
     6301 gtcgcaaaca cgaaatggaa cggctggtgc aaatcctgtg ccgccgccgc aaaaacaatc
     6361 cgcttttggt cggcgaagca ggtgtgggca aaaccgcgct ggcggaaggt ttggcacatc
     6421 aaatcgtcaa cagcgacatt cccgacgcgc tcaaagatac cgaagtgtac gcgctggata
     6481 tgggttcgct gttggcgggc acgaaatacc gcggcgactt tgaagcgcgg gtcaaatccg
     6541 tcttgaaaca gctcgaaaaa atcccgcacg ccattttgtt tatcgacgaa atccacacca
     6601 tcatcggcgc gggcagcacc agcggcggca ccatggacgc gtccaacctg ctcaaacccg
     6661 cgctggcaaa aggttcgctg cgctgcatcg gcgcgaccac ctacgacgaa taccgcacca
     6721 ttttcgacaa agaccatgcc ttaagccgcc gcttccaaaa aatcgacgtg gtcgaaccca
     6781 ccgtttccga aaccgttcaa atcctgcgcg gcttgaaacc gatgtttgaa gccttccacc
     6841 aagtccgcta cactcaaggc gcactcgaag ccgccgccga actctccgca cgctacatca
     6901 acgagcgttt cctgcccgac aaagccatcg acgtaatgga tgaagcaggc gcggcgcaac
     6961 ggattctgcc caaatccaaa cagaaaaaag tcatcggcaa agcacaaatc gaaaccgtca
     7021 tcgccaaagt ttcgcggatt cccgaaaaaa ccgtgtcgca cgacgacaaa caagtgctgc
     7081 aattcctcgg tcgcgatttg aaaaacatgg tttacggtca agaaaacgcc atcgacgcgc
     7141 tggttgccgc cgtcaaaatg tcgcgttccg gccttgccct gcccgacaaa ccgataggca
     7201 gcttcctctt ctccggcccg accggcgtgg gcaaaaccga agccgccaaa cagcttgcct
     7261 actcgctggg cgtaccgctg caacgctttg atatgtccga atacatggaa cgccatgccg
     7321 tatcgcgcct catcggcgcg ccaccgggct acgtcggctt tgaacaaggc ggccttttga
     7381 ccgaagccgt caacaaacag ccgcattgcg tattgctctt ggacgaaatc gaaaaagccc
     7441 accccgacat tttcaacgtc ctcctgcaag tcatggacgc aggcaaactg accgacaaca
     7501 acggcaagag tgccgatttc cgcaacgtca tcctgattat gaccaccaac gcaggcgcgg
     7561 aaagcctcag ccgccccagc ctcggcttta ccgccaagcg cgaacgcggc gacgaaatgc
     7621 aggcaatcaa caagctcttc acgcccgagt tccgcaaccg cttggacgcg attatcccgt
     7681 ttgcgccctt gtccgaaccc atcatcgtca aagtcgtgga caaattcctg ctccagctcg
     7741 aacaccagct cctcgacaaa aaagtcgaag ccgaattcac gtcggcattg cacaaatatc
     7801 tggcggaaaa aggtttcgac ccgcaaatgg gcgcgcgccc gatgcaccgc ctgattcagg
     7861 aaaaaatccg caaaccgctc gccgacgaac tcctgttcgg caaactcgcc gacggcggct
     7921 ttgtgcggat agactgggat acggcaaaag aagaagccgt gttgaagttt aagaaaagca
     7981 aggtcaaaat
//
LOCUS       DAWFIE010000088         7938 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWFIE010000088 DAWFIE010000000
VERSION     DAWFIE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.088"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(214..285)
                     /locus_tag="WGQ71_001772"
     tRNA            complement(214..285)
                     /locus_tag="WGQ71_001772"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(250..252),aa:Arg,seq:cct)
     gene            complement(305..751)
                     /locus_tag="WGQ71_001773"
     CDS             complement(305..751)
                     /locus_tag="WGQ71_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ0707739.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(755..1729)
                     /gene="ispB"
                     /locus_tag="WGQ71_001774"
     CDS             complement(755..1729)
                     /gene="ispB"
                     /locus_tag="WGQ71_001774"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ0707740.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(1745..1933)
                     /locus_tag="WGQ71_001775"
     CDS             complement(1745..1933)
                     /locus_tag="WGQ71_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707741.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            1953..2261
                     /gene="rplU"
                     /locus_tag="WGQ71_001776"
     CDS             1953..2261
                     /gene="rplU"
                     /locus_tag="WGQ71_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ0707742.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2286..2558
                     /gene="rpmA"
                     /locus_tag="WGQ71_001777"
     CDS             2286..2558
                     /gene="rpmA"
                     /locus_tag="WGQ71_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ0707743.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            2947..4131
                     /gene="ubiM"
                     /locus_tag="WGQ71_001778"
     CDS             2947..4131
                     /gene="ubiM"
                     /locus_tag="WGQ71_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ0707744.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTAVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAQPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(4402..4557)
                     /gene="rpmG"
                     /locus_tag="WGQ71_001779"
     CDS             complement(4402..4557)
                     /gene="rpmG"
                     /locus_tag="WGQ71_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ0707745.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(4589..4822)
                     /gene="rpmB"
                     /locus_tag="WGQ71_001780"
     CDS             complement(4589..4822)
                     /gene="rpmB"
                     /locus_tag="WGQ71_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ0707746.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(5072..6598)
                     /gene="farB"
                     /locus_tag="WGQ71_001781"
     CDS             complement(5072..6598)
                     /gene="farB"
                     /locus_tag="WGQ71_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ0707747.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKCRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVTALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     FVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(6622..7776)
                     /gene="farA"
                     /locus_tag="WGQ71_001782"
     CDS             complement(6622..7776)
                     /gene="farA"
                     /locus_tag="WGQ71_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ0707748.1"
                     /translation="MDTHADETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELIQAVRQNRRQNAATSQAAAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEIESTDWSEVDRTVDEILGQSAP"
BASE COUNT         2146 a         2188 c         1846 g         1758 t
ORIGIN      
        1 aatttaatcc actatatcat aaaacaacag atttccaagc cggaacatct tttacggagc
       61 ctgaaaatca tttcatgatt ctaccgtcct aaaggtcggt ttttcaagca ggaagaaaaa
      121 gttttcagat ggcaaaaaag ccctccaaca ctgaaaggcc ttatatcgga aacttcccgc
      181 aacacgggaa acagacaaat gcaaatcgtc aaacctcgcc aacaggaatc gaacctgtat
      241 tttacgctta ggaggcatac gttctatccg ttgaactatg gcgagccgaa atgaaaagga
      301 gattttaacc ctttccgacg acaaaagaca agatgccggc cgcaatcagc ggcccgacag
      361 ggataccgcc cataaatgcc accccgataa ccgtcccgat taacagccct gtaattaaaa
      421 caggctgctg tcccatcaaa ggcacgccgc gtcccgccag ccaagccacg aaaatcccga
      481 taaaaacggc ggatatcatt ttaaaattca aaaattcggc aacgggagga acctgcgcct
      541 ttcctgaaac caacggactc aaaaccccta tggtcaaaag aatgataccg agattcaacc
      601 cgtgcttctc gaccaacggg acaaactgta ccaatgccgt ctgctgcatc agcagcaata
      661 tggttgccga gatggtaatc gaattgttgt tgctgaccac ccccaacaga atcagcgtaa
      721 ccaaaaacag gggaacaaaa ctgaaattca tcggttacga caccctgacc aaagattcct
      781 tcgccagctg aatcatggca tccttcactt cgctgtcggg cagggcatcc aaggcggtaa
      841 cggcacaatc gactgctttg cgcgcctcgc ctatcgaata tgccaaagca tccgaacgga
      901 cgacataatc gtggattttc tcaaaatagc tgcgatctgc attttccaaa gcagtacgca
      961 catcgttcgc aacctgttcg gaaccctgac gcatcagata aatcaaaggc aaagtcggtt
     1021 ttccttccgc caaatcgtcg ccgacgtttt tgccggtttc ttcggtttcg ccagaatagt
     1081 ccagcacatc gtcaataatt tggaatgccg taccgacata cataccgtag tctttcaacg
     1141 cccgttcgtg ttcgggggaa gccttgccca aaattgcgcc gacttgagcg gcagcttcaa
     1201 acaattttgc cgttttatat tggatgactt ggatatattg ttcttcggta atgtccgtat
     1261 tgccgatgtt catcagctgc atgacttcgc cctcggcaat aatgttggtt gcatccgcca
     1321 tcacttccaa aacgcgcata ctgcccgagg caaccatcag ttgaaaggcg cgcgtgtata
     1381 aaaagtcgcc aaccaacaca gccgccgcat tgccgaacag attgtttgcc gttgcccgcc
     1441 cacggcgcaa atcgctttca tcgacgacat cgtcgtgcag gagggtggaa gtgtggataa
     1501 actcgaccat cgccgccagc gaatacagtt tctcgtcatc ataaccgacc gccttacccg
     1561 ccaaaatcgt cataatcgga cgcaggcgtt tgccgcccgc gctgatgata tatgtaccga
     1621 tttgcgaaat cagtgcgaca tcggattgca ccgcacggtt gatgacttca ttgactttgg
     1681 caaggtcttc aggcagatgt cgctggaaat agggcagatt ctcgagcatg gcagactctc
     1741 aggtttacgt ttccgtcaaa cggcagatac cgcttgaaag ctgattcaaa tagcgggcaa
     1801 ttatatcagc aaagccatca gggtacatct tttcagacgg catatggtta tccgaaccgc
     1861 gcaaactttg acaaaacagg caaataacaa tacaatgccg agttccgcac aatggtgtgc
     1921 ggatattaac cataaccctt atggagttga gtatgtacgc ggtcgtaaaa accggcggca
     1981 aacagtataa agtttccgtc ggcgaaaaat tgaaagtaga acagatacca gccgaactcg
     2041 acagccaaat cgaactgacc gaagttttga tgattgctga cggcgaatct gtaaaagttg
     2101 gcgcaccctt tatcgaaggt gcaaaagtaa cggctaaagt agtggcacac ggtcgtggcg
     2161 aaaaagtccg catcttcaaa atgcgccgcc gcaaacacta ccaaaaacgc caaggccacc
     2221 gccaaaattt cacccaaatc gaaatcgtgg caatcgccta accttaaaac ttaggagcat
     2281 tacaaatggc aagtaaaaaa gcaggcggca gcacccgcaa cggtcgcgat tcagaagcca
     2341 aacgcttggg cgttaaagcc tacggcaacg agctgattcc cgcaggttcc atcatcgtac
     2401 gccaacgcgg taccaaattc cacgcaggcg acaacgtagg tatgggcaaa gaccacactt
     2461 tgttcgccaa agttgacggt tatgtcgaat tcaaaaccaa aggcgcgctg aaccgtaaaa
     2521 ctgtcagcat ccgtccttac accggttctg aagaataatc caaccgatac ctgaagccgc
     2581 atcttttcac gatgcggctt tttatagtgg attaacaaaa atcaggacaa ggcgacgaag
     2641 ccgcagacag tacaaatagt acggaaccga ttcacttggt gcttcagcac cttagagaat
     2701 cgttctcttt gagctaaggc gaggcaacgc cgtactggtt taaatttaat ccactataca
     2761 taccccacac ccgatttgat acacattaag ctgaaagtaa aaatccgcat acaccctccc
     2821 ctgcatattt cttcaacagt gggttttgat ataatcgcct atctgttaca gatagttcaa
     2881 acggcattca aacccttaca aataccgtct gaaatcatcg gcatccgtct atgcaaagga
     2941 tattttatga gtttacacag cgacatcctc gtcgtcggcg caggcccggc gggtttaagt
     3001 tttgcagccg aacttgccgg cagcggtttg aaagttaccc tgatcgaacg cagcccactg
     3061 actgttctgc aaaatcctcc atatgacgga cgcgaaatcg ccctgaccca tttttcccgc
     3121 gaaatcatgc agcgtttggg tatgtgggac aaaattcccg aaaacgaaat ctaccctttg
     3181 cgcgatgcca aagtgctgaa cggacgttcc gactaccagc tccactttcc ccaaccgacc
     3241 gaggcgcgcg gcgagcctgc cgactgtttg ggctatctga tttccaacca caatatccgc
     3301 cgcgctgcct acgaagtcgt atcccaactc gacaacatta ccattctgac cgatacggcc
     3361 gttaaagaag tcaaaacatc tgacaacgaa gcacaagtca ttttagaaaa cggcaaaatc
     3421 ctgaccgccc gcctgctttt ggcggcagac agccgttttt cgcaaacccg ccgacaactc
     3481 ggcatttctt cagatatgca cgactacagc cgaaccatgt tcgtctgccg catgaaacat
     3541 actctttcca accaacatac cgcttacgaa tgcttccatt acgggcgcac catcgcactg
     3601 ctgccgctgg aagaacacct gaccaatacc gtcattaccg tcgataccga taaaatcaac
     3661 agcgttcaaa acctttcgcc cgaagaattg gcggcaagcg taaaagaaca gctcaagggc
     3721 aggctcggcg atatggaatt ggtcagcagc atccaccatt acccccttgt cggtatgatt
     3781 gccaaacgtt tctacggcaa acgcagcgcg ctgatcggcg atgcggctgt cggtatgcac
     3841 cccgttaccg cacacggttt caacttgggt ctgtccagcg cagatatttt ggcaaaactg
     3901 atactcgaag ccgagcagcg cggtcaggac atcggcgcgg cttccttgtt ggaaaaatac
     3961 agcagcaaac atatgctgca tgcccagccg ctttatcacg gaaccaatat gatgctgaaa
     4021 ctgtttacca atgagaccgc gccggcaaaa ctgctgcgcg gtttggtatt gcgtgccggc
     4081 aacaacttcc cgccgctgaa aaaactgatt accaaacaac tgaccggttg aaaactgccg
     4141 aaatgccgtc tgaaacgttt cagacggcat ttttgtatta tagtggatta acaaaaatca
     4201 ggacaaggcg acgaagctgc agacagtaca aatagtacgg aaccgattca cttggtgctt
     4261 cagcacctta gagaatcgtt ctctatcctt ggcagacggt ttgcagcaga ttcgcatacg
     4321 gcagatgttt caagcggaaa ggaaacacga taaaaataaa aaagcctccg aacagtcgga
     4381 ggctttgctt tcaaactgaa attatttcag tttggtttct ttgtacacta cgtgtttgcg
     4441 ggcaaccgga tcgaattttt tgatttccag tttgccgggc atagtgcgtt tgtttttggt
     4501 agtggtgtag aagtgaccag tacctgcact ggattccagt ttgattttat cgcgcattgc
     4561 agtaatcctt aattaaatag tgtttaaatt aagcttcgcc gcgagcacgc aaatcagcca
     4621 atacgacatc aatgcctact ttgtcgatgg tacgcagtgc agcgttggaa acgcgcaggc
     4681 gaacccagcg gttttcactt tctacccaaa aacgacgtga ttgcaagttg ggcaaaaaac
     4741 ggcgtttggt tttgttgttg gcgtgcgata cgttgttgcc ggacatcggg cgtttgccgg
     4801 tcactttgca aactcgtgcc ataatcagtc tccaaacttc taaaatagta aaacgcgatt
     4861 tataccacga aaaacagtgt tagttcaagt gttttaaaga aaaacggctg ttttgcgcgg
     4921 ataatccttc cgattgtcgc atctgtggaa cgtttgcagg tgcgaaacag atggggcgcg
     4981 attatagcgg atttcgggaa ctgctgcatt caaaatgccg tccgaacata tttccagtat
     5041 tttcagacgg catttcaagt tttcaaatcc ctcaatgtcc accgccgccg ccgttgtgga
     5101 acggcggttt tgccagccat atgacgggta tcatgataat gaataacaag ctgcccgcca
     5161 taaagatttc gttcgagccg ataataaaac cctgctgggt aatggtattg ttgatgatgc
     5221 ctagggtttg aatgtcggaa acgccgtgct gggacaaatg agcggccgtt tcgtgcaatg
     5281 ttgcggaata gggcgtgatg tgttcggcaa agcgtgtatg gtgcaacgct tcacgccgtt
     5341 cccacagggt actaacgacg gatacgccga caccgcccat cagcacgcgc aagaaattcg
     5401 acaggctgcc tgcggcggca atctgcccgc ccttcatatg cgacagtgtg atggtggtca
     5461 gcggcagaaa aaacatggcg acaccgacac cctgccaaaa ctgcggccag atgacgttgc
     5521 caatatccat atcggcataa aaatccgtac gccaatagaa agtaaaggca aaggtcagga
     5581 agctggcagt tacgaacagg cgcatatcga ttttattgcc gaacctgccg attaacggag
     5641 acaggaaaac aggcaggatg ccgacgggcg cggcggcaag ccctgcccac gtggaggtat
     5701 agcccaggtt ggtctgcaac actaacggca gcagggtcag cgtccccata tacaccataa
     5761 aacccaatga cgtggcaatg acgccgacgg taaaattccg atctttaaac agcgataaat
     5821 cgacaatcgg atatttttct cccaattccc aaacaataaa atacgacaag cacaccagtg
     5881 cggttacgcc caaggtaatg atttctccag aggcgaacca gtcgagttcc ttacccctgt
     5941 ccaacatcat ctgtaacgcg ccgataccga ctaccatcaa tgtaagcccg acatagtcgg
     6001 tcggcatttt aacggtttcc gtttcccgac atttcaaatg tttccatgta atccatgccg
     6061 atatgatacc gatagggata ttaatgaaga aaatccaacc ccaatgccag tttccggaaa
     6121 tccagccgcc gagtatcggc ccgagaacag gggcgacaac gacggtcatt gcccacaatg
     6181 ccagtgccag cgtccgtttt gcgggcggat aggatgccat taacaggctt tgcgacaagg
     6241 gaatcagcgg cccggcgata aagccctgca agatgcggaa aacaaccagc gactgaaggt
     6301 tgggggcaat accgcacagc cacgatgtga tgacgaaacc gacagcggcg gcggtaaaca
     6361 atttgacctc gccgatacgt tttgccaaaa agcccgttaa cggcacggaa acggcgtttg
     6421 ccacagcaaa agaagtaatg acccacgtcc cctgcgtggt tgccgcaccg aggttgccgg
     6481 cgatgacggg aacggcgaca ttggcgatag tcgtatctaa aacttccata aatacggcaa
     6541 gccccaaaga cagcgtaacc cacgccaatg ccgcaccctt aagcggtgga taatccattg
     6601 tgtcctccgt ttcagacggc atcagggcgc ggattgcccg aggatttcat cgaccgtccg
     6661 atcgacttcc gaccagtcgg tactttctat ttccggcaat gccgcacccg gcgtttttga
     6721 aacaggcgcg cctgcggcgg aagtatccac tttaaccgtc atcgacaaac cgatacgcaa
     6781 cggatgcctg tccacatctt cgcggttcag cacgatacgg acggggacgc gctgcaccac
     6841 tttaatccag ttgcccgttg cgttttgcgc cggaatcagc gaaaacgcgc tgcccgtacc
     6901 tgccgaaaaa cctgccaccc tgccgcgata aacaatttgt ttgccgtaca aatcggacac
     6961 cagctcggca ggctgtccga ttttcatatg ccgcaactgc gtctctttaa aattagcatc
     7021 cacccacaca tccgacagcg gcaccaccgc catcagcggc gcgcctgccg ccacctgctg
     7081 cccgacctgc accgaacgct tcgccacctg accgtccgcc ggcgcgcgga tttgcgtccg
     7141 ctgaaggttc aaccacgcat ctttcaacct gccgattgcc gtctgaaccg ccggctgttc
     7201 gcgcaaagaa acctgaccgc ccaaagccgc acgcgccgaa gattcttccg ccaaagccgc
     7261 cttaactgcc gcctgagcct gagacactgc cgcacgggcg tgtgccagct cttcagccga
     7321 caccgcgccc gattccgcca aagcagaccg gcggcgcaaa tcatcctgcg cgcgcgccaa
     7381 atccgcccgg cgcaaggcaa cctgtgccgc cgcctgcgaa gtggcggcat tttgccggcg
     7441 gttttgccgc accgcctgaa tcagctcgtt ttttgcccgc tcgtaagcca gcacatcatt
     7501 atcgtcgtcc aataccgcca gcacgtcgcc ttttttcacg gcatccgtat cgtcgtgcaa
     7561 aaccttccgc accgtaccgc ccttttgcgg cgtaacctga accacgcgtc cggcaacata
     7621 agcgtcttcc gtttcctctt cgtgctgcca ccataaaaaa aacgccgacc cggcggctgc
     7681 ggcggcaagc gcgaacagca gcgtcaatgc cgtcaggcgg cgtttgcgtt tggcttgcgt
     7741 gttttgaagt tttgtttcgt ccgcgtgcgt atccataaga ttgggttccg aatttcctga
     7801 tttcatataa acaatgttct ttcaatagga agcagattct atatccgcgc aataacgcaa
     7861 tcaagtcagt attttagatg caatattcaa aaaacaacgc ttcaggcggc aaaactgaaa
     7921 atatatttcg tttatata
//
LOCUS       DAWFIE010000089         7456 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWFIE010000089 DAWFIE010000000
VERSION     DAWFIE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.089"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            157..330
                     /locus_tag="WGQ71_001783"
     CDS             157..330
                     /locus_tag="WGQ71_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0707749.1"
                     /translation="MKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
     gene            394..1677
                     /gene="hemL"
                     /locus_tag="WGQ71_001784"
     CDS             394..1677
                     /gene="hemL"
                     /locus_tag="WGQ71_001784"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ0707750.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGIMPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FQTAADAAGIEFAADSVGGMFGLYFAAHAPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1893..2165)
                     /locus_tag="WGQ71_001785"
     CDS             complement(1893..2165)
                     /locus_tag="WGQ71_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HEZ0707751.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(2176..2739)
                     /gene="orn"
                     /locus_tag="WGQ71_001786"
     CDS             complement(2176..2739)
                     /gene="orn"
                     /locus_tag="WGQ71_001786"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ0707752.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2757..3644)
                     /gene="prmA"
                     /locus_tag="WGQ71_001787"
     CDS             complement(2757..3644)
                     /gene="prmA"
                     /locus_tag="WGQ71_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ0707753.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQTAAQECGLKDLAYTGEILEDQDWVR
                     LTQAQFDPIRISERLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEESWARLSGTKR"
     gene            complement(3762..5123)
                     /gene="accC"
                     /locus_tag="WGQ71_001788"
     CDS             complement(3762..5123)
                     /gene="accC"
                     /locus_tag="WGQ71_001788"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ0707754.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(5237..5692)
                     /locus_tag="WGQ71_001789"
     CDS             complement(5237..5692)
                     /locus_tag="WGQ71_001789"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ0707755.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(5674..5931)
                     /locus_tag="WGQ71_001790"
     CDS             complement(5674..5931)
                     /locus_tag="WGQ71_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ0707756.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6048..7088
                     /gene="queA"
                     /locus_tag="WGQ71_001791"
     CDS             6048..7088
                     /gene="queA"
                     /locus_tag="WGQ71_001791"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ0707757.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LSDYVGEGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLHRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT         1548 a         1877 c         2220 g         1811 t
ORIGIN      
        1 cagacggaag agggaatcgc acaaacggtt gttaaaggca aaacgacccg ggcggaggta
       61 gaggcacgtt tcgggaagcg caaccctttc ggttgttatg cctatcatga agtcagcctg
      121 ccgatttata attttttgcc gaccaatttc atttatatga aatcagaacg ccggcattgg
      181 gagtggtgtg tggattacga ctgggaagga gttgtccggg actaccgctt tacacataaa
      241 gtggaaaaag acgagcgttc cgtcatccgg gatgcggttg gcgtcatccg caaagaagca
      301 ggcaaatcct tgtcgcaacc tgaaaaatga taaaatggga ctttctgctt ctaagcccga
      361 aacctgccgt tcagacggca tttgaggata aatatgaacc gtaacgaaat tttattcgac
      421 cgcgccaaag ccatcatccc cggcggcgtg aattcccccg tccgcgcatt cggcagcgtc
      481 ggcggcgtgc cgcgcttcat caaaaaagcc gaaggcgcgt atgtttggga cgaaaacggc
      541 acgcgttata ccgactatgt cggctcttgg gggcctgcga ttgtcggaca cgcgcacccc
      601 gaagtcgtcg aagccgtgcg cgaagccgcg ttgggcggtt tgtcgttcgg cgcgcccacc
      661 gagggcgaaa tcgccattgc cgaacaaatc gccaaaatca tgccgtccgt cgaacggctg
      721 cgcctcgtca gctccggtac cgaagcgacg atgaccgcca tccgtttggc acgcggtttt
      781 accggtcgcg acaaaatcat caagtttgaa ggctgctacc acgggcattc cgacagcctg
      841 ctggtgaaag ccggcagcgg cctgctgacg ttcggcaatc cttcttccgc cggtgtgcct
      901 gccgacttta ccaaacacac tttagtcctt gagtacaaca acatcgccca attggaagaa
      961 gcctttgctc aaagcggcaa tgacattgcc tgtgtgattt tggagccttt tgtcggcaat
     1021 atgaaccttg tccgcccgac cgaagccttc gtcaaagccc tgcgcgaatt gaccgaaaaa
     1081 cacggcgcgg tgttgattta cgacgaagtg atgaccggtt tccgcgtcgc gctcggcggc
     1141 gcacaatcgc tgcacggcat catgcccgac ctgaccacga tgggcaaagt catcggcggc
     1201 ggtatgccgc ttgccgcgtt cggcgggcgc aaagacatta tggaatgtat ttccccactg
     1261 ggcggcgtgt atcaggcagg tacattatca ggcaacccga ttgccgtcgc cgccggtttg
     1321 aaaacgctgg aaatcatccg ccgcgaaggg ttttatgaaa acctgacggc gcgtaccgaa
     1381 cagttggttc aaggttttca gacggcagcg gatgcggcgg gcatcgagtt tgccgccgac
     1441 agcgtgggcg gtatgttcgg tctgtatttc gccgcacacg cgccgcgaaa ctatgccgat
     1501 atggcgcgtt ccaatattga cgctttcaaa cgcttcttcc acggtatgct cgaccgcggc
     1561 attgccttcg gcccgtccgc ttatgaagca ggtttcgttt ctgccgcgca tacgcccgag
     1621 ctgattgacg aaacggttgc ggttgcggtt gaagtgttca aggcgatggc tgcatgatgt
     1681 tttgacggac agagtttctc tgttcgattt gtttggcaga ttgaagtaag aatgcacacc
     1741 gccgtcattc ccgcgcaggc gggaatccag acctttcagt ttctgtaatg attgaaaata
     1801 acgggccgaa atcagccgcg cccggcagga agccgccgcc acctgaaaca atgccgtctg
     1861 aagccttcag acggcatttt atcggaagcg catcagaaaa aacggatagc gacatatgcg
     1921 gtaaaagcaa gggcaattac atagataaac ccctttataa attcaaaagt ctcatctata
     1981 tcgctatatt tttctttcag ttcgtcagat tttcgataac gtttcatttt caagaccact
     2041 tcatgaccaa caagcccgac cttccggatt cccgcctgcg cgggaatgac gggcgtgtat
     2101 atttttgatt tcaatctgct gtaaaaatgc cgtctgaaat gcaggatttg cgcttcagac
     2161 ggcatttttt gtttcttatt gcgcttcggc tctcggggcg gaaatcagaa agtgttcgcg
     2221 gtagtggcgc atttcttcga tgctctccaa aatgtcgtcc aatgccttgt gcgaaccgcg
     2281 tttgacgacg cttttggcaa cgggcggatt ccagcgtttg gcaagctctt tcagtgtgga
     2341 aacgtcaagg ttgcggtagt ggaagtagtt ttccagtttc ggcatatatt tgaccataaa
     2401 acgccggtct tggtggatgg aattgccgca catcggcgtg gcgcgtccgg gtacccattc
     2461 cgacataaag tccagcagtt tctgttcgac ttcggcttcg gtatgcgacg attcgcgtac
     2521 gcgctgtgtc agccccgtcc tgccgtgtgt ggcggtgttc cactcgtcca tattgtcgag
     2581 cagctcgtca ctttggtgga cggcgtaaac ttcggattgc gccaatacat tcaaatccga
     2641 gtcggtaata atcatcgcga cttcgataat gcggtcggtt tcgggattaa gccccgtcat
     2701 ttccatatcg agccagcaga ggttgttttt gtcctgcacg gtgtttcctt tccgtttcag
     2761 cgttttgtcc cgctcaagcg cgcccagctt tcttcggttt ccgccgggtc gagatcgaac
     2821 cattggctgt aaatgccgcc gagttcttcg gcttgttcgt ccaacaaacc ggataacacg
     2881 atgcggccgc cttgtttggt gcgggcggcg agcatttcgc cgagcatacg taaagggttg
     2941 gcgaggatgt tggcgacaac tacgtcgaat tgcccttgag gcagaccgtc gggcaggaag
     3001 aattgtgcat cgacgttgtt ctgttcggcg ttgtccttgc cggcgcggac ggcttgttcg
     3061 tcaatatcca cgccgacggc agaacctgcg ccgagtttga gggcggcgat ggtcaggatg
     3121 cccgaaccgc agccgtagtc gaggacgctt tcgccgtttt tgagttgcgt atccagccat
     3181 ttgaggcaga ggcgcgtggt cgggtggctg ccggtgccga aggcgaggcc gggatcgagg
     3241 cgtaggttga cggcagtgcc ttcgggggct tcgtgccaag agggggtaat ccacaggcgt
     3301 tcggaaattc ggatggggtc gaattgcgct tgggtcaggc gtacccagtc ttggtcttcg
     3361 aggatttcgc cggtgtatgc caagtctttt aatccgcatt cttgtgcggc ggtttggatg
     3421 atggcggcgg cttcgtcgtg ttcgccgaac agggcgatga ctttgctctg ctgccagatt
     3481 tgttcggcgg gcataccggg ttcgccgaaa atcgcctgtt cgttttgcgt gccggcgtag
     3541 gcatcttcga tggcggcgga gagtgcgccg tgttccatca gcgcgtcggc gaggcgttcg
     3601 gcgacggcat cgttgacgtt gacggtgatt tgttggtagg gcataacggg ctttcttaac
     3661 agaccttcgg aatagggtcg tactgtataa ggggatgggt gtgtaacgga aatgccgtct
     3721 gaaaacggac aaactgtttc agacggcata tcggcggcgg tttacttgtc ctgtttggct
     3781 ttgcgatctt ccagccagtg ttccaaatag tggatgctga caccgccttt ttggaaaccc
     3841 gcatcggcga acaggtcgcg gtgaagcggc gtattggttt tgatgccggt taccgccagc
     3901 tcggcgagtg cgacgcgcat tttcgccatt gcctgttcac gcgttttgcc gtgtacgcag
     3961 attttgccga tcaggctgtc gtagtacggt gggatgcggt agccttggta aatgtggctg
     4021 tccacgcgga taccgaagcc gccgggcagg tggcagcttt caatcgggcc cgggctgggg
     4081 atgaagttgt atgggtcttc ggcgttgata cggcactcaa acgcgtggcc ttcgacttga
     4141 atatcctttt gtttgtattg caaaggcagg ccggcggcga tgcggagttg ctcctgcacg
     4201 atgtccacgc cggtgatgag ctcggtaacc ggatgctcga cctgaacgcg cgtgttcatc
     4261 tcgataaaga aaaattcgcc gtcttcgtat aaaaactcaa acgtacccgc gccccggtag
     4321 ccgatgcgtt tgcaggcatc ggcacaggcg ttgccgattt tggcgcgttc tttttcagtg
     4381 atgaacggag ccggtgcttc ctcgatgact ttttggtggc ggcgttgcag cgaacagtcg
     4441 cgctcggcaa ggtagatggc gttgccgtgt tcgtcggcaa tcacttggat ttcgacgtgg
     4501 cgcggacgtt gcaaatagcg ttccatgtaa accatcgggt tgccgaatgc cgcgcccgct
     4561 tcggccttgg tcatttcgac agattggagg aggtcttctt ttttctcgac cacgcgcata
     4621 ccgcgaccgc cgccgccacc ggaggctttg ataatgacgg gataaccgac tttatcggcg
     4681 attttgagga tttcttcgcc gtcgtcgggc aatgcgccgt cagaaccggg gacgcagggt
     4741 acgcctgccg ctatcatcgc gtgtttggcg gagactttgt cgcccatcag gcggatggtg
     4801 tcgggtttcg ggccgataaa ggtaaagccg gactgctcga cctgttcggc gaaatcggcg
     4861 ttttcggcaa ggaaaccgta gcccggatgg acagcgtccg cgcagcttac ttcggcggcg
     4921 gcgataatgg cggggacgtt aaggtagctt tgcgcggaag cggcagggcc gatgcacacg
     4981 gattcgtcgg cgagtttgac gtgcaggctg tctttgtcgg cctcggaatg cacggcgacg
     5041 gtggcaatgc ccatttcgcg gcaggcacgg agtacgcgta atgcgatttc gcctcggttg
     5101 gcgattaaaa cttttttcag catgatgact gttcctgtgg ttccggcaac ccgtccgtaa
     5161 aaaaaaaggt gcgggaacgc cgaaggggga aagcatttca gacggcatcg gaagtttatg
     5221 ccgtctgaaa acaggattat ccgatgatga agagcggttc gccgaattcg acgggcgtac
     5281 cgttttcgac cagaatttct ttgaccgtgc cggatttttc ggcttcgatt tcgttcatca
     5341 gcttcatcgc ttcgatgatg cacagcgtgt cgccggcttt aacctgttgg ccgacttcga
     5401 caaaaggcgc ggcattcggg ccgggtgcgc ggtagaacgt gccgaccata ggcgattttt
     5461 gcgcgtcgga caaatcgcgg gcggcaggtg cggcggcggg cgcggatgcc gcaacgggtg
     5521 cggcggcagg cgttacggcc ggcgcggcgg caggtacggg cgcggcgtaa acgggtgcgg
     5581 cggcgatggt tcgggtgatg cggacttttt cctcgccttc ggttacttcg atttcggcga
     5641 tacccgattc ttcaaccaaa tcaatcagtt tttttaattt gcgcaaatcc atttctgttc
     5701 ctttaaaggc tgccggtgtt ccggcgggtt gttgcgttgg tttttcagaa aacagccggc
     5761 ttgcggcaac ggcgcaaacc gggcggaatg gtgttcggat acggttttcc tgtggtggaa
     5821 aacaaccgtg agaatgtgtt tattttcccg aagttgtcgg taaatgtcca gtaaaatatc
     5881 aaaaaacggc ggtttttggg ggaaatgtgc aggaagtttg attttgcgca caagatgttc
     5941 caggttaaga gtgtttattc taatctgttg gtttttcgga caaagatgcc gtctgaaaaa
     6001 gggctaaagt gcgtataatg gcggcttgcc cgaacgagag tgtaaaaatg gatatttcag
     6061 attttgattt taccctgccc gaaaagctga ttgcccagca tccgcccgaa gtgcgcggca
     6121 gcagccgcct tttggttgcg ttgcccgata tgccgctgca agaccgggtg ttcggcgatt
     6181 tatccgatta tgtcggtgag ggcgacgttt tggtgttcaa caacaccaaa gtcatgaaag
     6241 cgcggctgtt tgggcagaaa gacagcggcg gcaggatcga agccctgatt gagcgcgtgt
     6301 tggacaacca taccgcactg gcgcacatcc gttcgtccaa gtcccccaag cccggtatgg
     6361 ggctggtgtt tgaaggcggc atccgtgccg tgatggtcgg gcgtgagggc gaactgttct
     6421 gcctgcgttt tgaaggcggt caaaccgttt acgaactttt ggaacagaac ggacacctgc
     6481 ccctgccgcc ttatatcgaa cgtgccgccg acgcggacga cgacagccgt tatcaaaccg
     6541 tttatgccaa atatcagggt gcggtcgctg cgccgacggc gggcctgcat tttacggaag
     6601 aacttttgca ccgtctgaaa gacaaaggcg cggtaaccgc agaagtaact ctgcacgtcg
     6661 gtgcggggac attccaaccc gtgcgtgtgg acaaaatcga agagcataaa atgcacagcg
     6721 aatggtttga agtgccgtct gaaaccgccg ccgccgttga ggcggcaaaa gcccggggga
     6781 acaaagtctg ggcggtcggc acgacttcca tgcgcgccct cgaatctgcc gcgcgtgcga
     6841 cgggtcgtct gaaagcgggg cagggcgaca ccgatatttt catcacgccg ggctaccgtt
     6901 ttaatgttgt cgacaggctg gttaccaatt ttcatctgcc gaaatcgacg ctgctgatgc
     6961 ttgtcagcgc gttttcgggt atgggtcata tccgcgccgc gtatcgttat gcggtcgaac
     7021 gcgaataccg tttcttcagc tacggcgatg cgatggtttt ggggcggaac gaaggggtgg
     7081 tacgttagaa cagccccaag tctattgggg gaagacaagt gccgtctgaa cggacgttcg
     7141 gacggcattc ttttgttgat ttgaatgaaa ggaactgtat gacatagatt caggcgcgag
     7201 aggcgagaaa ctcagtcatt gttgatgacg cgcaatacgt taaaattcat tccgttttgg
     7261 tcgtataaac atattacata cgccattttt ttgtattcaa ggctgcctga aaacagtggg
     7321 caagaatatc taacccaacg aaaagctgaa aaagcaacaa aaaataatct gcaccttagt
     7381 cggttggtta gtccaacctt aggggtgcag accattcaat ctttcaggct gcctttgttt
     7441 gtttataaga taaatt
//
LOCUS       DAWFIE010000090         7447 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWFIE010000090 DAWFIE010000000
VERSION     DAWFIE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.090"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(205..2808)
                     /gene="pepN"
                     /locus_tag="WGQ71_001792"
     CDS             complement(205..2808)
                     /gene="pepN"
                     /locus_tag="WGQ71_001792"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ0707758.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSTKLLSVKINGVAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GECSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPARYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNIFELTVKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQTFQFEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLAVPSEAELWDGT
                     ENIDPLRYHQAREALLDTLAAHFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHVED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(3020..3814)
                     /locus_tag="WGQ71_001793"
     CDS             complement(3020..3814)
                     /locus_tag="WGQ71_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0707759.1"
                     /translation="MTTILAFDIETVPDVQCIRTLYDLPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLHGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWSEFVQAWK"
     gene            complement(4563..5432)
                     /locus_tag="WGQ71_001794"
     CDS             complement(4563..5432)
                     /locus_tag="WGQ71_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ0707760.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYRLVKPRRRVGEIN
                     LAKCFPEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDEAL
                     SAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDAQILKGRNRYDNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWESFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5483..6019)
                     /gene="ruvC"
                     /locus_tag="WGQ71_001795"
     CDS             complement(5483..6019)
                     /gene="ruvC"
                     /locus_tag="WGQ71_001795"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ0707761.1"
                     /translation="MSATVRILGIDPGSRVTGFGVIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQPDAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(6022..6261)
                     /locus_tag="WGQ71_001796"
     CDS             complement(6022..6261)
                     /locus_tag="WGQ71_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ0707762.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6291..7301)
                     /gene="dusB"
                     /locus_tag="WGQ71_001797"
     CDS             complement(6291..7301)
                     /gene="dusB"
                     /locus_tag="WGQ71_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ0707763.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHKNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEAAYDLIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAEKTDSWACAYRPN
                     AF"
BASE COUNT         1673 a         1966 c         1946 g         1862 t
ORIGIN      
        1 acaaaaacca gtacagcgtt gcctcgcctt accgtactat ctgtactgtc tgcggcttcg
       61 tcgccttgtc ctgatttttg ttaatccact ataattaata tgactttgcg gccgttttgc
      121 cattgcgtaa taaaacgatg gggaagtgat gataaaacgt gtgtgtaact atatcagaca
      181 gcattgtttt tctgtttgac ggcctcaatc caaaattttg ccgacgattt cgcccacgtc
      241 tttcgacaat ccttcctgcg cccgaatgcg ctgcaatgct tgtttcacca agtttttgcg
      301 gtgcggctcg agcttgttgc agaggttgaa cgcctgcacc aggcgggcgg cgacctgcgg
      361 gttaaagcgg tcgatttcga taactttgtc ggcgataaag cggtagccgc tgccgtcttc
      421 tacgtggaaa tgcgggacgt tgcggctgaa gctgccgata agcgatcggg ctttgttggg
      481 attttcgagg ctgaacttcg gatgctgcaa ggcggtttga acctgttgca gggtgtcgct
      541 gcggcggctt gagccgacga gggcgaaata tttgtccatc accagcgcgt cgtctgaaaa
      601 cttgtcggca aactgcgcca gcaggcggtt gcgcgtatcg ctttcgttgc cgttgacggc
      661 ggacaggatg ccccattcgt gggtcatgtt ttgcgccatt tcgccgtatt tttcggcaac
      721 ggtttcgatg tgcgcggggt cggcgcgcag gacgaaggcg cggcagacgt tgcgcagcgt
      781 gcgccagccg gcggcttcgg ggctgtattc gtaactttgg ttttcctgct tcgccgcctg
      841 acggttcaat tcgtgccatt tcggcaggaa gtgggcggca agcgtgtcca acaaggcttc
      901 gcgcgcctga tggtagcgca acgggtcgat gttttccgtg ccgtcccaca gctcggcttc
      961 agacggcacg gccaaaagca gggctttgaa ggcgttgtcc aagaggtcgt ctgaaatgac
     1021 tttttcgacg gcggcaagca gtttttcgtg tttcggcaac ccgatgccgt ctgaaagcgc
     1081 ggcgaggttg gcggcgacgg cgcggcggta gagcgtttgg gcggcttccc agcgcgtgaa
     1141 ggcgtcgctg tcgtgggcga gcaggagcag caggtcgtcg tcgctgtacg gatagttcag
     1201 atgcactggc gcgctgaagc cgcgcagcag cgagggaacg acggcttcgg tcacgccttc
     1261 gaactggaag gtttgttcgg cttcggtcag cagcaatacg gcttcggtcg cgcgtttgcc
     1321 ctgatagtcg aatgccgccg cttcgccgtt gcggttcagc agcccgattt tgacgggaat
     1381 catcatcggc tgcttgtccg ccatatcggg cgtaggcggc acggtttgtt tgacggttaa
     1441 ctcgaaaata ttgtttttca gacggccttc ggcttccaaa acgggcgtgc ccgcctggct
     1501 gtaccacaag gcgaactggt cgagattgat gccgttcgcg tccgccatcg ccgcgcggaa
     1561 atcgtcgcag gtcacagcct gtccgtcgtg gcgttggaaa tagagcttca tgcctttttg
     1621 gaagccctct tcgccgagca aggtgtgata catccgcacc acttccgcgc ctttttcata
     1681 aacggtcatg gtgtagaaat tgttcatctc ctcatatcgg gcggggcgca ccggatgtgc
     1741 ggtcggacct gcgtcttcgg ggaactggtg ctggcgcaaa aggcggatgt tttcgatgcg
     1801 gcgcacggcg cggctggcgc ggtcgccgga aaattcttgg tcgcggaaca cggtcagccc
     1861 ttccttcagc gaaagctgga accagtctcg gcaggttacg cggttgcccg tccagttgtg
     1921 gaaatattcg tgtccgacca cggattcgat gccttcaaaa tcggtatcgg tggcggtgcg
     1981 gctgtcggca aggacgaact tggtgttaaa gatgttcaaa cccttgtttt ccatcgcacc
     2041 catattgaaa tcgcctacgg cgacgaccat gaaaatatcc aagtcgtatt ccaagccgaa
     2101 gcgcgtttcg tcccacttca tcgcgttttt caaggattcc acggcaaagc cgaccttggg
     2161 cttgtccgct tcggtggtgt aaaactcgat tttgacgttt ctgccgctca tggtggtgaa
     2221 atagtcttcc gtgaccgcca aatcgcccgc aaccaaagca aacagatagc tcggtttggc
     2281 aaacgggtct tcccatttca cccaatggcg gccgtctgaa cactcgccgc cgtcgatttt
     2341 gttgccgttg gagagcaaaa cgggatagcg ttttttgtcc gcaacgatgg tggtcgtgaa
     2401 tttggacatc acatccggac ggtcgatgta gaacgtgatt ttgcggaagc cctccggctc
     2461 gcattgggta aacagattgc caccggaagc atacagcccc atcagcgatt tgttttccgc
     2521 cggcaggatc tcggtttcca cttcgacggt gaagcgttcg gacggcacgt ctgcaatcgt
     2581 cagagtctcg ccttccaaca cataatccgc cgccacgccg ttgattttga cggacaagag
     2641 tttcgtcgaa ccgtccaaca ccagcggctc ccccaccctc tgcggctcga ccgtcaaacg
     2701 ggatttcacg acagtttgcg gttcggcaat atcaaaatgc aggtcggttt caaggatgcg
     2761 gtaggcaggc gtttggtaat ctttcagata acggacggtt ttgctcattt ttttctttca
     2821 atattgtttg gtttgactgg aaaaggcttc agacagcacg gatacagctt ttaaagcgga
     2881 ttaacaaaaa tcaggacaag gcgacgaagc cacagacagt acagatagta cgggctgtac
     2941 cggtttgaat ttaatccacc atatgccgtc tgaaaccgaa acggcggcac aagcgtaccg
     3001 ccttcggaca gcaggttttc tatttccacg cttggacaaa ttctgaccaa tgcggtttat
     3061 cttctgtttg ggcggaaaga tagtttctga tccgcttgat ttccatttcg tattcgtccg
     3121 cgtcgaatct gccgctgacc agacaaaaac gcagatacat caaatacgta ttcacggcat
     3181 cggtttcgca ataattgcgg atttctttca gcctgcccgc gtggaacgca tcccacacct
     3241 tgctgccgtc catacccagc ttgcccggaa aaccgcacag tttcgccata tcgtccagcg
     3301 gcacgtttgc cctcggctgg taaagcgcga gcaaatccat caaatcgcag tggcgttggt
     3361 gataacggct gatgtagttg ttccacttga aatcgcggct gtcgccgaaa tcgccgtcgc
     3421 ccatatccca atagcgcgcg gcgttgatgc cgtgtagcag cgaacggtaa tgcagaaccg
     3481 gcaggtcgaa accgccgccg ttccaactga ccagttgcgg cgtatgtttt tcaatcaact
     3541 cgaaaaattt ggcgataacc acttcctcgc cgtcatccat ctcgccgatt gtaccgacat
     3601 ggactttatc ctgcccccaa cgcatgcagc acgaaatcgc cacaatctga tgaagatgat
     3661 gctgcataaa atccccaccc gtctgagcac ggcgtttttg ctgggcaaac agcaccactt
     3721 catcgtcggg cagcgaggac ggcaagtcat acagcgtacg gatacactgc acatcgggta
     3781 cggtttcaat atcgaaagcc aaaatcgtgg tcatgacagc accttgtatt taaaacagac
     3841 ttgcgcctat tgtgtcatta aaaggcggat aaaaaaagag gcaacccccc acaggattgc
     3901 cccaatacct caaatcagag atttacgctt cacaaacaat acaggctttc gcctgcggct
     3961 ttgcccgcgt agctcaactc tacgccggca aacttttgtt tcaccgtttc cgatgaaacc
     4021 ccgaccaatc gcaagattga ccggaaaatc ctttcagacg gcatttcctg cctgtcgtgt
     4081 aattccatgt agcgaaatgt acgccatttt ctacgctttg ccaagcattt tttacaatat
     4141 aaatgtcaaa acattaattt tataaaattg ctgaaaatat taaatatatg aatttttatt
     4201 ttaaatattt taataaatat aaatttaatt ttgatttata tttaaattta agcataaaat
     4261 gtcaaatatt aaaggaaata tgaaaagcat atattaaata tttatttata acgctatgtt
     4321 tttaaagaaa attaatttta atatattaac tagattgtct gcagatattc atgggtttgc
     4381 ggtatttctt ccaaaacctg cttcgaattt tccgaccaag tcttaaaaat attgtttttg
     4441 agatacttaa atagcagcga ttatcaaatg aaatctgttc atataatcta ccatttcgca
     4501 tttaaaaaaa caatcaggag tttcgactcg aaacgcctga tatgttttgt aattttacgt
     4561 agtcagtaaa aatcggggct gccttccgga cgggttttga aacgcttgtg cagccaaaaa
     4621 tactgctcgg gatgttcgcg cacgcgttcc tcgataaaac ggttcatgcg ctgcgcgtcg
     4681 gcctgcgcat cttcactcgg aaaggattcc caagccgggt agaaatgcaa tgtaaccgta
     4741 ttgtccgcct cgcggacggg gatggcgggt atcacttttg catttgcaag cgcggcaatg
     4801 cggctcaatc cggtaatcgt tgccgtctga ataccgaaaa aatccacaaa aaccgaatcg
     4861 ttgcgtccga aatcctgatc gggcagatac agaaacggcg cgctgctttt gcggaactgt
     4921 ttgacgaggg cgcgcagccc ttcggtgcgc ccgatgagga agacgttgtc gtagcggttg
     4981 cggcctttca aaatctgtgc gtccaatatc ttgttttttt ggtgggaata catactgatc
     5041 agcggaacat cctgattgag cgcgtacacc gccatctcga acgcggtaaa gtgcggatac
     5101 aggatgatga ctttttcccc tgccgataat gcctcatcca gataatgttt gttgcggtaa
     5161 cgcaccagcg acttcaaacg tcctgcgggc gcataccagt ataaaccgta ctccaacatc
     5221 aacttcgcca tgtgtttgaa atgctgcttc aatacggttt tacgcttttc ctcactccat
     5281 tcgggaaaac attttgccaa attgatttcc ccaacccttc ggcggggttt gaccagacgg
     5341 taggcaagca aacccgtcag gtcggcaagt ttgtgcagca gcgcaaacgg cagaaactgc
     5401 aaaacataca gtacaaaaaa tataaatttc atctcgatac acattttctt ttcaggtggc
     5461 aaaatacaaa tgccgtctga aactattgaa acctgccgcg cttgacctgc atccccgaag
     5521 gattgagttt ggcggcaagc ccgtggttac gtaaggcgtg ggtcagcgcc accgccaaac
     5581 cgtcggcggc atccggctgg ggcgttcccg aaagtcccaa catctgcacc accatatgct
     5641 gcacctgttc ttttgccgcc ttacccttgc cgaccaccgc ctgtttgacc tgcaaggccg
     5701 tgtattccga aacgggcagc ttatggctga ccaatgccgc caatgccgcg cccctagcct
     5761 gacccagcat cagcgtcgat gccggattga cgttgacgaa cacctgttcc accgccgcct
     5821 gctgcggctt gtaaacggca acgacttcgc cgatgtgccg cacaatcacg gcaatcctgt
     5881 ctgccagagg cgcatcggca ggcgttttga tgcagcctga ggcaacgtaa aaatgatcgc
     5941 gccccctgac atcgatgaca ccgaaacccg ttacgcggct gcccgggtct atgcccaaga
     6001 tacggacggt tgccgacata ttcacagcaa accgtgttga atcagttttt tacgcaaggt
     6061 attgcggttc agccccagca tcacggatgc tttggactgg ttgccgccgc attgctccat
     6121 cacgcacacc agcagcggtt tttccacctg atgcaatacc atatcgtaca cgccgcaagg
     6181 ttcggtaccg ttcaggtctt tgaaatattg ttctaaattt tgtctgatgc attgggaaat
     6241 atcgggaagg gtatggggca tgattgcact ttcaaaggat aatcaagtgt tcagaaggca
     6301 tttgggcggt aggcgcacgc ccaactgtcg gttttttcgg caagtctttc aagataacct
     6361 gcaagcatgt cgtattgcgc cgccgcactg tccaagcggt tgatttcacg acgtgtctgt
     6421 tcgccgtcgg gcatttcgtc gatgtaccag cctatgtgtt tgcgtgcaat gcgcacaccg
     6481 gcggtctcac cataaaacgc gtgcatggcg cggacgtggt tcaaaatggc ggcactgcat
     6541 tctgccaaac tcaaggcagg cggtaaaaca ccgtgttcgg cgtaatgttt caaatcgcgg
     6601 aagaaccacg gtctgccttg cgcgccgcgc cctatcatga tgccgtctgc ggcggtttgt
     6661 ttaaggacgg cttgggcttt ttgcggcgaa gtaatgtcgc cgttgaccca aaccgggatg
     6721 ttcagacggc atttggtttc ggcaatcagg tcgtaagccg cttcgccttt gtacatttgc
     6781 gtgcgcgtgc gtccgtggac ggcaagggcg gcaatgccgc aatcttcggc gattttggcg
     6841 atgacgggca ggtttttgtg gtcgtcgtgc caacccaaac gggttttgag ggtaacgggt
     6901 acgcccgccg ccttgaccac cgcttccaaa atggcggcaa ccagcggctc gttctgcatc
     6961 agcgcgctac cggcttggac attgcagact tttttagcgg gacagcccat gttgatgtcg
     7021 ataagctgcg ccccaaggct gacgttgtaa cgcgcggcat ccgccatctg ctgcggatcg
     7081 cttccggcaa tctgcacggc aacaatgccg ccttcatcgg caaaatcgct gcggtgcaag
     7141 gtttttctag tatttctgag cgtcgggtcg ctggtcagca tttcgcacac cgcccaacct
     7201 gcgccaaaat ctcggcaaag tcggcggaac ggtttgtcgg taatgcccgc catcggcgca
     7261 agtgcgatgg ggttgtcgat aaaatagccg ccgatgtgca taatgggtcc gcgtttcaaa
     7321 aaagtgcgac attgtacatt ttttaagcag gatttccaat ctccggacgc gcccgcgatt
     7381 ggggcgggca ccgttttatg gcataatccg cacacagatt tcctgccccg ccactcacag
     7441 gcgggca
//
LOCUS       DAWFIE010000091         7430 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWFIE010000091 DAWFIE010000000
VERSION     DAWFIE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.091"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(190..468)
                     /locus_tag="WGQ71_001798"
     CDS             complement(190..468)
                     /locus_tag="WGQ71_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707764.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSLKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            complement(475..759)
                     /locus_tag="WGQ71_001799"
     CDS             complement(475..759)
                     /locus_tag="WGQ71_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707765.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            complement(767..964)
                     /locus_tag="WGQ71_001800"
     CDS             complement(767..964)
                     /locus_tag="WGQ71_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707766.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            complement(969..1259)
                     /locus_tag="WGQ71_001801"
     CDS             complement(969..1259)
                     /locus_tag="WGQ71_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707767.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGRSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            complement(1304..2646)
                     /locus_tag="WGQ71_001802"
                     /pseudo
     CDS             complement(1304..2646)
                     /locus_tag="WGQ71_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            complement(2731..2946)
                     /locus_tag="WGQ71_001803"
     CDS             complement(2731..2946)
                     /locus_tag="WGQ71_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707768.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRP"
     gene            complement(2947..3297)
                     /locus_tag="WGQ71_001804"
     CDS             complement(2947..3297)
                     /locus_tag="WGQ71_001804"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707769.1"
                     /translation="MQNGGGKIYQTADNVEGIMLLKVVPERTVSADAKTRDPMWDNAA
                     LQTSEGVNFIARFLGFFSDGEYRYVDVLQPNHSDIIRYSGKDFPLNQILNHIHPARYA
                     VTFENNVDSKLRRH"
     gene            complement(3234..3576)
                     /locus_tag="WGQ71_001805"
                     /pseudo
     CDS             complement(3234..3576)
                     /locus_tag="WGQ71_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3812..5239
                     /locus_tag="WGQ71_001806"
     CDS             3812..5239
                     /locus_tag="WGQ71_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ0707770.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     VVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            5308..6711
                     /locus_tag="WGQ71_001807"
     CDS             5308..6711
                     /locus_tag="WGQ71_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ0707771.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTGLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(6875..>7430)
                     /locus_tag="WGQ71_001808"
     CDS             complement(6875..>7430)
                     /locus_tag="WGQ71_001808"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0707772.1"
                     /translation="GDNAQKKTTTYKEQDPAKVTHYLTQPAEFSDYQRVYLDETGFDR
                     YLFRPYARSLKGQIVKAQISGKRYQRLSLVSAQVGNRLIAPMVYQNTMTGVFFEAWFQ
                     QCLLPALTQKSVIILDNARFHRMGVLREMAEKLGHKVLPLAPYSPELNPIEKVWANIK
                     RYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         1857 a         1782 c         1853 g         1938 t
ORIGIN      
        1 ttgctagacg gctgatattt ataaagtgcc aactgcgcct gagtgataaa cggcttgttc
       61 atggttctgc ctttcaatga ttgttttgaa agcctgattt tgacaccata acttcatgcg
      121 ctcaattctt aaacagaacc gccccgatta atacgggtac ggaaacgccg agataaaaat
      181 aaaaatccat catttcaaaa cctttttcag cagggaaaca aagtaaacgg acgcgaggat
      241 gccgaatact atccagcctg tttcaaggcc gctttgcagg ttgtctttca gactgcattc
      301 cgccaataaa agccttagcg gctgaccgtc cgacatcttc cacaggctgc cgttatattc
      361 cggcctgaca atctgtccgt tttctttgat tcttggtact accaagctga aataaaggtt
      421 ttcagcctgg tgcttctcaa gacatttatt tccgacttgg tagtacatgc cgtcttactt
      481 catcactctc ttaacgatgg aaaatacaaa aagcgcggcg aaaacgccaa cgacaatcca
      541 accggcttcc ataccgtccg cttttgcggc ttccaaagcg ttttttgccg tatcgggcaa
      601 cgttgcattt gcatgtgcgg ccaaagccag gggagcagct gttacaacag ccagttttgc
      661 gccgtattta cggcaggtgt taataaattt catgatattt tccttcaaaa agtgtttggc
      721 ggtaatggat ggagcgtttt tcagacgacc gccgaacatc cgaaaatcag tctttcaaaa
      781 atccgaatac gacaaattcg tattggttgc cgatttcttc caaacctgcg ttaatcgctt
      841 cttcgaagtc gtagaaataa tcggcattgg tgattaattt ggtatgtccg atgtcgcccg
      901 tttcaggaga gagatacaga aagtcccctg ttgatacgga ctggacaaca tagactttct
      961 gcattcaatc agcctttctt cacgagttga aaaccgatga ctttcagttt ttgggttttg
     1021 cccgtagtga cgatttctac gttcaggttt gcttcgatcg gaaattgggc gtttcggaac
     1081 tgctcgaaat tggcagagcc gccgaaatcg tattcagtag tagagctgcc caatgcgttg
     1141 ccttgggagc tgtctaaggg tgtggcgaca atcaggcggc aatagtcgaa gctcctgcct
     1201 tcgatttgtc cgttgaattt tttaacgccg acgatgtggc cttgaagttg gatgttcatt
     1261 ttttggtttc cttgtgtgat taaacgtctt tcggacagac actttaagcc catgaaatcg
     1321 gtagtcttgc gaatttgtcg taaatgaagt tgttatagct ttcttcattg ttgacgtgtt
     1381 tttgctgttc aagctgtttt tcaagattct cgtaatattc gtacatatag taagggtctt
     1441 tgtacggttt gaatgcgggc tgttcatgaa tggcttgagc tttcaaaaag gcgcagtcgt
     1501 aggcttcggg agccaaagac ttgggcagct tgtgatgact cggctcaatc agttcaaaca
     1561 gtttggcttt gtccaattcg ggaaaaatga atttcagacc gtttgccgca cgtccaaact
     1621 gtttttttac ccattcaagg tagcggtcgg ctgaaatgac cttatcttcc ttaaccgcgt
     1681 gtatgcgcgt tgccttttgg acgaatcgtt cgcaaatcgg atatgcgccg ccgaaatatt
     1741 cgcccggatt ctgcaaaact tcaaaaggga taacgatgtc ttttgctttg aattcaattt
     1801 caaagcgcgt ccatgtgctt gttttatcgc ccaactgctt gcctttttca tagacgcgga
     1861 catatttgga cgattcacgg gagccgatac cataggtctt gcctttggtc attttggctt
     1921 catcgtcttc ttcccaatct gaccccaaac attcgccttt tggtttgacg tgatgacagg
     1981 taaacatacc tttatttcgg tcttcacggg cttggttcgg gctgtattcg ccgttgaaaa
     2041 agtcttttgc gatgtcaacg cgtgtgattt ttgggcggat tgcattagtc aggaatgcga
     2101 aaagtcgtga ttcccagcct tcttttgcga cgccgcaacc ggtgccggtc agttcgaaaa
     2161 ggatggtatt gttttgtccg ccaaaatgga cgcgaccgta taggacgtct tccgaaccca
     2221 tcaaccaaca gcgctcatag aaacgaccgc ccgagccttt ggattctttg tagataccga
     2281 aaccgaaaac ttcttcggcg agcatggacg cggcgcgaat aaaatcttcg tcttccaaaa
     2341 gacttacacg aacgccgtat ttatcgaaaa aggttttttc atgaaatgaa aagctaattt
     2401 gatcaatgaa agccgaatct gatacaccgc gccgaagagg aacgcctaac aggtttcctt
     2461 taccgtccgt tatgtacgtt tcgtaacatt cgaagacttc ctgaaccctg cctgccgttt
     2521 cggtttctgt ccccccctgt tagataaggg gggaagattt gaagcggttg tcggcttcct
     2581 gccgtccgct ggcgcgtccg tcatcacgcc ggcaaccgcc tttgtcatcc cttgcttatc
     2641 ttccatggtg cgaatcctca aaaacgggca aaaaaaagcc ctgttacttg tagaaagtaa
     2701 aggacgttaa tttttgttaa tcgtcccttc ttagggacgc aatatataag gttttaaaac
     2761 gctaagaaca aaatccgata acggctctga agaagtaagt ctttctttat tgcacaagat
     2821 agcaaacttc cacggcattc tcgccccacc cgtgccgtcc aagccttttt caaagacata
     2881 gatggttttc ttggcaatca cttcgtcagt ttggcggtcg ataatccgta tggtcgcacc
     2941 tgccactcag tgcctgcgca gcttggaatc gacattgttt tcgaacgtta ccgcataacg
     3001 ggcggggtgt atatggttaa gtatttgatt tagcggaaaa tctttacctg aataccgaat
     3061 aatatcggaa tggttgggtt gcaggacatc cacatagcgg tattccccat cgctaaaaaa
     3121 tcctaggaaa cgagcaataa aatttacgcc ttcgctggtc tgtaaagccg cattgtccca
     3181 catcgggtct ctggtttttg catctgccga aacggtacgc tcaggtacta ccttcaacag
     3241 cataatccct tccacattgt ccgccgtctg gtaaatcttt cccccgccgt tttgcattgt
     3301 tcgttaaaca cggcttcggc ttctttgtat tttctgtccc actcttcttg tgcctgtatt
     3361 tcttctttgc tcgggcagaa ttgtttggga ataatccaaa caaacagcat caggatagcg
     3421 gcggcggtca ggctgcctga aaggattttg ccggggtttt ttgtaggcaa agcggacgag
     3481 aaaccaaagc aacagcagca tggtgtccca atagccgatt gagaatagga tggccaaacc
     3541 ttctaggaaa tggcgtaaat cgtttgtggt aaacatgggt agttcctgtg gttaaatgtg
     3601 caggctgctt tttgccgaac cttgccgcat ctcaaaagca gcctgcgctt cagcgttgcg
     3661 ttacgcagta aaataatgaa tatttgtaac gacttgggta ttttttgtca atattcccgc
     3721 ctttccctta acagctgccg cgctttccgt taaaattcct ttacatattt atattgtttc
     3781 ctgtttctat attgccaagg ttatacccgt tatgtttttc tccgccctga aatcctttct
     3841 ttcccgctac attaccgtat ggcgcaatgt ttgggcggtg cgcgaccagt tggaaccgcc
     3901 caaacgcacg gcggaagaac aggcgttttt gcccgcgcat ttggaactga ccgatacgcc
     3961 ggtctctgcc gctccgaaat gggcggcgcg ttttattatg gcgtttgcgc ttttggcttt
     4021 gttgtggtcc tggttcggca aaatcgatat tgtggcggcg gcttcgggca aaacggtgtc
     4081 gggcgggcgc agcaaaacca tccagccgct ggaaacggtg gtggttaagg cggtacatgt
     4141 gcgcgacggg cagcatgtga aacagggaga aacgctggcg gaactggagg ctgtgggaac
     4201 agacagcgat gtggtgcagt cggagcaggc tttgcaggct gcccaattgt ccaaactgcg
     4261 ttatgaagcg gtattggcgg cattggaaag ccgtaccgtg ccgcatatcg atatggcgca
     4321 agcacggtct ttaggtctct ccgatgccga tgtgcaatcg gcgcaggtgt tggcgcagca
     4381 ccagtatcag gcatgggcgg cgcaggatgc gcaattgcag tcggctttgc gcggccatca
     4441 ggcggaattg cagtcggcca aggcgcagga gcagaagctg gtttcggtgg gggcgatcga
     4501 gcagcagaaa acagcagact accgccgttt gcgggccgac aattttattt cggaacatgc
     4561 gtttttggag cagcagagca aatcggtcag caattggaac gatttggaaa gtacgcgcgg
     4621 tcagatgagg cagattcagg cggccattgc acaggcggag cagaatcggg tgctgaatac
     4681 gcagaacctg aaacgcgata cgctggatgc gctgcgccag gcaaacgaac agattgacca
     4741 ataccgcggc caaacggata aggcaaagca gcggcagcag ctgatgacaa tacagtcgcc
     4801 tgcggacggc acggtgcagg aattggccac ctatacggtg ggcggtgtgg tgcaggctgc
     4861 ccaaaaaatg atggtggttg cgcccgatga cgacaaaatg gacgtggaag ttttggtatt
     4921 gaacaaagac atcggttttg tggaacaggg acaggatgcg gtggtgaaga ttgagagttt
     4981 tccctatacg cgctacggtt atctgacggg caaggtgaaa agtgtcagcc atgatgcggt
     5041 aagccacgaa cagttgggct tggtttatac ggcggtggtg tcgctggaca aacatacctt
     5101 gaatattgac ggcaaagcag tgaatctgac ggcgggcatg aatgtcacgg cggagattaa
     5161 aacgggtaaa cggcgggtgc tggattatct gttaagcccg ctgcaaacca aattggacga
     5221 aagctttagg gagcgatagg cggatccata ctgggcattt gttatccgcc ggttcggaca
     5281 tgcagactgc ctgaaaccat tgcccggatg acattgctca atctaatgat aatgcaagat
     5341 tacggtattt ccgtttgcct gacactgacg ccctatttgc aacatgaact attttcggct
     5401 atgaaatcct atttttccaa atatatccta cccgtttcac tttttacctt gccactatcc
     5461 ctttccccat ccgtttcggc ttttacgctg cctgaagcat ggcgggcggc gcagcaacat
     5521 tcggctgatt ttcaagcgtc ccattaccag cgtgatgcag tgcgcgcacg gcaacaacaa
     5581 gccaaggccg cattccttcc ccatgtatcc gccaatgcca gctaccagcg ccagccgcca
     5641 tcgatttctt ccacccgcga aacacaggga tggagcgtgc aggtgggaca aaccttattt
     5701 gacgctgcca aatttgcaca ataccgccaa agcaggttcg atacgcaggc tgcagaacag
     5761 cgtttcgatg cggcacgcga agaattgctg ttgaaagttg ccgaaagtta tttcaacgtt
     5821 ttactcagcc gagacaccgt tgccgcccat gcggcggaaa aagaggctta tgcccagcag
     5881 gtaaggcagg cgcaggcttt attcaataaa ggtgctgcca ccgcgctgga tattcacgaa
     5941 gccaaagccg gttacgacaa tgccctggcc caagaaatcg ccgtattggc tgagaaacaa
     6001 acctatgaaa accagttgaa cgactacacc ggcctggaca gcaaacaaat cgaggccata
     6061 gataccgcca acctgttggc acgctatctg cccaagctgg aacgttacag tctggatgaa
     6121 tggcagcgca ttgccttatc caacaatcat gaataccgga tgcagcagct tgccctgcaa
     6181 agcagcggac aggcgcttcg ggcagcacag aacagccgct atcccaccgt ttctgcccat
     6241 gtcggctatc agaataacct ctacacttca tctgcgcaga ataatgacta ccactatcgg
     6301 ggcaaaggga tgagcgtcgg cgtacagttg aatttgccgc tttataccgg cggagaattg
     6361 tcgggcaaaa tccatgaagc cgaagcgcaa tacggggccg ccgaagcaca gctgaccgca
     6421 accgagcggc acatcaaact cgccgtacgc caggcttata ccgaaagcgg tgcggcgcgt
     6481 taccaaatca tggcgcaaga acgggttttg gaaagcagcc gtttgaaact gaaatcgacc
     6541 gaaaccggcc aacaatacgg catccgcaac cggctggaag taatacgggc gcggcaggaa
     6601 gtcgcccaag cagaacagaa actggctcaa gcacggtata aattcatgct ggcttatttg
     6661 cgcttggtga aagagagcgg gttagggttg gaaacggtat ttgcggaata aagcaggctg
     6721 aaacggttat gaaattccca aagcagcctg caccccgttt cgaaagtgca ggctgctttg
     6781 ggattgatcc gatactttca cattctcatt gcctttaaat aaagcacagt taaaaacaca
     6841 tttaaattaa gtgcgaatta attcaaatgt atattcaatt aaaatcaaaa taggacagta
     6901 gtgcatcgtc aaatcgggcg taatcagaca aaacggttcg cagataccgc ttaatattcg
     6961 cccacacttt ctcaatcggg ttgagctcag gtgaataagg tgcaagaggc aataccttat
     7021 gtcccaattt ttccgccatt tcccgtaaga cacccatacg gtgaaatcgc gcattatcta
     7081 aaataatcac cgatttttga gtcaatgcgg gcagtaggca ttgctgaaac cacgcttcaa
     7141 aaaagactcc ggtcatcgta ttttgataaa ccatcggagc aatcagccgg ttgccgactt
     7201 gtgcggacac caaagataag cgttggtatc tttttccact tatctgcgct ttcactattt
     7261 gccctttcag gctgcgggca tagggacgga acaggtagcg gtcaaatcct gtttcatcca
     7321 aataaacacg ttggtagtcg gaaaattcgg ccggctgtgt caaataatgc gttactttgg
     7381 ccgggtcttg ttctttgtaa gtggtggtct ttttttgcgc gttatcccca
//
LOCUS       DAWFIE010000092         7396 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWFIE010000092 DAWFIE010000000
VERSION     DAWFIE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.092"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(151..573)
                     /locus_tag="WGQ71_001809"
     CDS             complement(151..573)
                     /locus_tag="WGQ71_001809"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ0707773.1"
                     /translation="MSIMEVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            complement(584..1981)
                     /gene="atpD"
                     /locus_tag="WGQ71_001810"
     CDS             complement(584..1981)
                     /gene="atpD"
                     /locus_tag="WGQ71_001810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ0707774.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            complement(2019..2894)
                     /gene="atpG"
                     /locus_tag="WGQ71_001811"
     CDS             complement(2019..2894)
                     /gene="atpG"
                     /locus_tag="WGQ71_001811"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ0707775.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            complement(2919..4466)
                     /locus_tag="WGQ71_001812"
     CDS             complement(2919..4466)
                     /locus_tag="WGQ71_001812"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ0707776.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIYGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            complement(4477..5010)
                     /locus_tag="WGQ71_001813"
     CDS             complement(4477..5010)
                     /locus_tag="WGQ71_001813"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ0707777.1"
                     /translation="MAEFATIARPYAKALFGLAQEKSQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            complement(5015..5485)
                     /locus_tag="WGQ71_001814"
     CDS             complement(5015..5485)
                     /locus_tag="WGQ71_001814"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ0707778.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            complement(5556..5792)
                     /gene="atpE"
                     /locus_tag="WGQ71_001815"
     CDS             complement(5556..5792)
                     /gene="atpE"
                     /locus_tag="WGQ71_001815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ0707779.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            complement(5849..6715)
                     /gene="atpB"
                     /locus_tag="WGQ71_001816"
     CDS             complement(5849..6715)
                     /gene="atpB"
                     /locus_tag="WGQ71_001816"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ0707780.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            complement(6705..7058)
                     /locus_tag="WGQ71_001817"
     CDS             complement(6705..7058)
                     /locus_tag="WGQ71_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ0707781.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
BASE COUNT         1949 a         1967 c         1676 g         1804 t
ORIGIN      
        1 ctatatgttt cggttaggcg gcaggctgcc ctattgaata ccttaaagca ggctatgcct
       61 gccaacgcca tatccaaaca cagtctttaa tttaaatccg gaaaataaaa agcacgacca
      121 aacggtcgtg cttttccaaa ccaaacaagt ttatttcttg tgcgaacgga tatagtccaa
      181 agttttgagc tgtgcaatcg cagcagccaa tgctttatgt gcttccgcca aagccttatc
      241 gtctttagct tgggaaatgc ccgcttctgc ggcttttttc gcctcttccg cacgtgcctg
      301 atccatctcc gcactgcgga cggcaacatc cgccaagaca gttactttat caggctgtac
      361 ttccaaaaca ccgccggaaa cagcaaccaa aacctcttta tcctcgcccg gaacggtcaa
      421 acgcaaagcc cccggccgca ccaaactcat aatcggctcg tgtcgcggat aaataccgag
      481 ttcgccctgt acagtcggaa caacgataaa tgttgcctcg cctgaataga ttttctgctc
      541 gctacttacc acctcaactt ccatgatgct catgccgacc tcctcagttt aaggttttcg
      601 ctttctcaac cgcttcttcg atgctgccga ccatatagaa tgcctgctcg ggcagatggt
      661 cgtattcgcc gttcaagatg gctttgaagc cggcaatggt atcgcgcagg gcgacatatt
      721 tgcccggaga acctgtaaac acttcggcaa cgtggaacgg ttgggacagg aagcgttgga
      781 ttttacgcgc acgcattacg gtcagtttgt cttcgtcaga caattcgtcc atacccaaga
      841 tggcgatgat gtcgcgcaat tctttgtatt tctgcagggt ggactgtacg ccgcgcgcca
      901 cgtcgtagtg ttcttgaccc aagaccatcg gatccagttg gcgtgaagta gagtcaagag
      961 ggtcaactgc cgggtaaata cccaaagagg caatatcacg gctcaatacg acggttgcgt
     1021 ccaagtgggc gaacgttgtt gccggagacg ggtcggtcaa gtcgtccgca ggtacatata
     1081 cggcttggat ggaagtaata gaaccggttt gggtagaggt aatacgctcc tgcaaacgac
     1141 ccatttcttc agccaatgtc ggttggtagc ccactgcaga cggcatacgg cccaacaatg
     1201 cggatacttc ggtaccagct agagtgtaac ggtagatgtt gtccacgaag aacaatacgt
     1261 cgcggccttt gccgttttcg tctttttcgt cacggaagta ttccgccatg gtcaaaccgg
     1321 tcaatgcgac gcgcaaacgg ttgcccggag gttcgttcat ctgaccgtaa accattgcca
     1381 ctttatccaa tacgttggaa tctttcatct cgtggtagaa gtcgttacct tcgcgggtac
     1441 gctcacccac gcctgcgaac acggacaagc cgctgtgcgc tttggcgatg ttgttgatca
     1501 attccatcat gttcacggtt ttacccacac cggcaccgcc gaacagacct actttaccgc
     1561 ctttggcaaa cggacacagc aagtcaatca ctttaatgcc cgtttcgagc aattcggttg
     1621 tggaagacag ttcgtcaaac ttaggagcgg cttggtggat ggcacgactc ttgtcggtat
     1681 cgatcggacc tgcctcgtca acaggcgtac ccaatacgtc gacaatacga cccaatgtac
     1741 ctttacctac cggcacagta atgggcgcac cggtattgct cacagtcatg ccgcgtttca
     1801 aaccgtccga gctgcccatc gcaatggtac ggactacgcc gtcgcccaaa agctgttgga
     1861 cttccaaagt cagaccgttt tcgtctaatt tcaaagcgtc gtaaacgcgc ggaatcatgt
     1921 cgcgtggaaa ttccacgtca acaaccgcac cgataatttg tacgattttg ccttggctca
     1981 ttatcgtatc ctaatttctg tacaggattc agacggcatc agacagccgc cgcacctgct
     2041 acaatttctg acaattccgt ggtaatcgca gcttgacgcg atttgttata taccaaacgc
     2101 aactctttga tggcattgcc tgcattgtct gttgcagctt tcatggcaac catgcgggct
     2161 gcctgttcgg atgccatatt gtcgctcaac gcctgataaa ccacagactc taaatagcgg
     2221 cgaaccagat attccaacac tgcaagtgca gtcggttcgt agcggtattc ccagctgaac
     2281 ggtgatttgg gagctgaatc gccaatcacg ttctcaccga taggcagcaa tacttccatt
     2341 ctcggttctt gacgcatggt attgacaaaa cccgaataca ccagatggat tctgtcaatt
     2401 tcatgtttct cataccgttg gaagagttct gtcaaaggtc cgagcagcat ttccattttt
     2461 ggggtatcgc ccaaatttac ggcactggca accacattca gaccaatgct ctgacacgcc
     2521 atcagacctt tactgccaag gcatacgatt tcctcttcaa taccttgatt ccgatactct
     2581 tgaacttgtg ccaaaaactt tttcagcacg ttggcgttca aaccgccaca caaaccctta
     2641 tcagacgtaa tcaaaataaa accgacacgt ctaatctccc gatgagattc cagtaacgga
     2701 ataccatgat cggtattggt ttgcgcaaga tggctcatca ccatacgcac tttttcggca
     2761 tacggacgcg ccaaacgcat ccgttcctga gtcttccgca ttttagaggt tgacaccatc
     2821 tgcatcgctt tagtgatctt ttgggtattc tgaacactgc ggattttggt gagaatctct
     2881 tttcctactg ccatttcaga ctcctttcac ttcaagcctt atgcctgata ggcgtaagaa
     2941 gatttgaagg atttcatggc tgcttcaagc gttttctcgc tctcgtcgga cattgcacct
     3001 gaagcattga cggcttccaa aacttccgga tgttgggtac ggacaaagct caaaaattca
     3061 gattcaaaag ccagagcttt ggcaaccgga acatcagaat acgaaccgtt gttgattgcc
     3121 caaagggtca aagccatttc agccgtattc aacgtactga actgtttctg tttcatcagt
     3181 tcggttacga cttcgccatg ctccaattgt ttgcgcgtag cttcatccaa atcggatgca
     3241 aattgcgaga acgccgccaa ttcacgatat tgtgccaacg ccaaacggat accgccaccc
     3301 agctttttaa tcactttggt ttgtgcagca ccgcctacgc gggatacgga aataccggca
     3361 ttgattgcag gacggatacc ggcgttgaag aggtcggttt ccaagaaaat ctgaccgtcg
     3421 gtaatcgaaa tgacgttagt cggaacgaaa gcagatacgt cgcccgcttg ggtttcgata
     3481 atcggcaaag cagtcagaga accggttttg ccttttactt cgccgttggt caatttctcc
     3541 acttcgtgtt cattgacacg tgctgcacgt tccaacagac gggagtgcag gtagaacaca
     3601 tcgccgggat aggcttcgcg gccgggcgga cggcgcaaaa gcagggaaat ttgacggtaa
     3661 gccacagcct gtttggacaa atcgtcataa acaatcaagg catcttcgcc gcggtcgcgg
     3721 aagaattcac ccatcgtaca accggagtaa ggtgcgatat attgcaatgc cgccgcttca
     3781 gatgcagttg cagcaaccac aatcgtgtgt tccatcgcgc catgctcttc caatttgcgg
     3841 accacgttgg caatagaaga tgctttttga ccgatagcga catagataca gataacaccc
     3901 gtaccttttt ggttgacgat ggcatccagc gctacggccg ttttaccggt ttgacggtcg
     3961 ccaataatca actcacgctg accgcgaccg acaggaacca tagagtcaat cgccttcaga
     4021 ccggtttgca tcggctggtc aaccgatttg cgcgcaatca cgcccggtgc gattttttcg
     4081 ataggggcgg tcaaagttgt attaatcggg cctttgccgt cgataggccg acccaatgca
     4141 tcaacgacgc gtccgaccag ttcgcgtccg accggcactt ccaagatacg accggtacag
     4201 gtaaccgtgt cgccttcttt aatatgttcg tactcgccca acactacggc gccgacggag
     4261 tcgcgctcca ggttcatcgc caagccgaaa gtgttacccg ggaattcgag catctcacct
     4321 tgcattgcat ctgacaaacc atagatgcga acgataccgt cagttaccga aatcaccgta
     4381 ccacgggtac gcacttcggc atttacagac agattttcga tcttggcttt aatcaaatcg
     4441 ctaatttcag caggattaag ctgcatgaaa actctcctaa ttcgtcatag tcgtgtacaa
     4501 ggcactcaat ttgccttgta cagacaaatc caaaacctga tcacccactt caacttttat
     4561 gccgccaatc agctccggtt cgatttcgac agagattttc agctcgctgt cgaaacgctt
     4621 attcagcatt tgcgccaact cgccgacctg tttgtcggtc aacggatagg cactgtaaat
     4681 gacggcagat ttgatatggt tgaatgataa ggtcaagtct tgatattgag catatacttc
     4741 cggcaatatc gacaaacgtt tctgcccggc caagacgata acaaagtttt tcaactcctt
     4801 gtctttcaaa ccgaccaaat cgatgaggat atctgctttt tctgaagcat tcgtttcagg
     4861 acggtcaatc aatgaagcca ccttcccttc ctgaacaacc gccgcaagtt tttccagtcc
     4921 gcccaaccaa gactcaattt ggcttttttc ctgagccaga ccgaacaatg cctttgcata
     4981 aggtctggca atcgttgcga actctgccat aagattacag ctcctgtttc agggtatcga
     5041 gcagttttgc gtgtttggaa gcatcgactt cgctgcgcaa aatagattcg gcacctttga
     5101 cagccaacac ggcaacctgc tcgcgcaggg attcgcgtgc gcggaacaat tcctgctcca
     5161 catcggcctt tgcctgagct gcaatgcgcg ccgcctcgga agaagcctgt tctttggctt
     5221 cttcgacaat tttggcggca cgtttttcgg cgttggcaac catttcggaa acctgattac
     5281 gcccttctgc caagagttct gcaacctttt tttcagcctg ctcgaaatcg cttttaccac
     5341 gctcggcagc tgccaagcct tcggcgactt ttgcggcacg ctcatccaaa gcttttgcaa
     5401 tcggcggcca cacgaatttc atggtaaacc ataccaaacc gaaaaagacg atgatttgag
     5461 cgaataatgt tgcattgata ttcacgttac ttaaccttcg tattggggtt aatcaaacag
     5521 gctgcgcctg tacggaacgg acgaatccgt cctgattatg cacctgcaaa cgggttaacg
     5581 aaggcgaaca gcagtgcaat ggcgacacca atcaagaatg cggcatcaat caaaccggca
     5641 atcaggaaca gtttggtttg cagcggaccg atcagttcgg gctgacgggc agaagactcc
     5701 aaatatttag aaccgaccat tgcgataccg atagaggcac ccagtgcgcc caatgcaacg
     5761 atcaaaccac atgcgatagc aatcaaaccc attttaaact ccttaaagaa acaaaggtta
     5821 aactacaaaa acaaactact taggaaaatc agtgcgcatc atgtgcctgt ccgatataga
     5881 cgaacgccaa cgccatgaaa ataaacgcct gcagggtaat caccaaaata tggaaaatcg
     5941 cccatgccaa accggcaata atgtggaata caaacagaat cggatccatg acttcgacgc
     6001 tgccggaagc cgcccaagca ccgccaagca aggctatcaa caagaatacc aactcgcctg
     6061 catacatatt gccgaacaac cgcataccgt gggatacggt tttagaaaga aactcgacca
     6121 aattcagcag gaagttcgca ggtgcgagtt ttgcaccgaa cggcgcgctg aacaactcgt
     6181 gaaaccatcc gcccaatcct ttgattttga tgttgtaata gatacaaatc agcaacacgc
     6241 cgacagcgag tgccaaagtg gtgttcaaat cggcagtcgg tacgacgcgc aacagggcgt
     6301 gatggttgcc ggtaatgccc tgccatacca tcggcagcaa atcgaccggc agcatatcca
     6361 tcgcgttcat caggaaaatc cagacaaaca gcgtcagacc caacggcgcg acggcttttc
     6421 tagacttttc gttgtgaatg atgctcttac acatatcgtc cacaaactca aacaagattt
     6481 ccacggcggc ctggaaacgt ccgggaacgc ctgccgtcgc tttttttgca ccgcgccaca
     6541 acaggaagct gccgattacg cccaacagga cggcaaaaaa gacggcatca aggttaataa
     6601 acgaaaaatc agcaatgttt ttcagtccct gaccctgagt aacatccgac aaactggtca
     6661 agctctgcaa gtggtgcttg atgtagtcgg cagcggtaat ggtttcacct gccataatct
     6721 ttcactctca acaatactaa aaaaaccaaa tggctgacac cgagcagccc catcagaaac
     6781 ggggcgaaca ccagcgattg atgccataca gcaaatacgg cgagcatgga caacagcgac
     6841 agcactactt ttaaaatctc tccgaagacg aacatcctgc tttgcaggaa ggggtttccc
     6901 ctgaaaagtt ttaaaagtaa aactgcaaca aacgtgggaa gcaggtagga caaaccgcca
     6961 ccgaccgccg aaaggaatcc ggcaaaaccc catacggcaa aggcaactgc ggcgcatatg
     7021 gacaatacgg cggattgtag gatgataatc tgcttcataa agggaatgtt tccgcctcga
     7081 atttgaggcg cggctaatat aatttagaag ccttattacg tcaagcaata tgttaatctt
     7141 tgtgaaacaa cgtatcccaa tccgccgcgc tcgccgcctg aataacggcg acaggtgtca
     7201 ttctaacaca cattacatat aattacagga tattaaggag tttgtccgca atttctttac
     7261 acttttaatg ttcttacgtg atttgtttgc tttacgtgga aataataaaa aatcaacgcg
     7321 aaattgtagc agtttatcgg tcggattgtc ggcagtttgg ggaatttgct caataaataa
     7381 aaggtcgtct gaaaat
//
LOCUS       DAWFIE010000093         7169 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWFIE010000093 DAWFIE010000000
VERSION     DAWFIE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.093"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(52..807)
                     /locus_tag="WGQ71_001818"
     CDS             complement(52..807)
                     /locus_tag="WGQ71_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ0707782.1"
                     /translation="MKFARLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGD
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQRNGSNEPGYNRTILDWYA
                     DGGRKDLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASVGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(934..2094)
                     /gene="nirK"
                     /locus_tag="WGQ71_001819"
     CDS             complement(934..2094)
                     /gene="nirK"
                     /locus_tag="WGQ71_001819"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ0707783.1"
                     /translation="MKRQALAAIIASMFALAACGGEPAAQTPAASAEAASSAAQTAAE
                     TPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWTFDGD
                     VPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTSTFSFK
                     ALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKGKKGA
                     QGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLVSSFH
                     VIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRAFNKG
                     ALGQLKVEGAENPEIMTQKLSDTAYAGNGAASAASAPAASAPAASESEKSVY"
     gene            2464..4719
                     /locus_tag="WGQ71_001820"
     CDS             2464..4719
                     /locus_tag="WGQ71_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ0707784.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPDETFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            4898..5431
                     /locus_tag="WGQ71_001821"
     CDS             4898..5431
                     /locus_tag="WGQ71_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ0707785.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5494..6678)
                     /locus_tag="WGQ71_001822"
     CDS             complement(5494..6678)
                     /locus_tag="WGQ71_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ0707786.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNVGFTVTNQQAGSRRF
                     QRFAGTDTQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDIGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDSSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(6718..7122)
                     /locus_tag="WGQ71_001823"
     CDS             complement(6718..7122)
                     /locus_tag="WGQ71_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ0707787.1"
                     /translation="MTAQPCPICTAQDEDILLQTPNLRVIAIHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1583 a         1756 c         2007 g         1823 t
ORIGIN      
        1 aggcgaggca acgccgtact ggtttttgtt aatccactat aattgaaggg gttatcggct
       61 tgtgcaacgg aagcccaagt tgtgcaagac atatttggat tgcaggctgg tgcggatgcc
      121 gtagcggagg aaggcggcat agttggacga gtcgctcgac ccgacagatg cgccgctgca
      181 aaacatttgg gcgttggcat tgccggaaga aagcaggctg ctgttgaaat cttccgtcca
      241 ttcccaaatc agaccgtgca tatcataaac gccccagtag ttcgggcgac ctttgccgac
      301 atcgtgcagg tctttccgtc cgccgtcggc ataccaatcg agaatagtgc ggttgtagcc
      361 gggttcgttt gagccgtttc tctgcgtggc ggaagcaagt ccggcaaatt cccattcgtc
      421 aatggtcggc aggcgtttgc cttgtgcggc gcaataggcg ttggcggcga accaggaaac
      481 attggttacc ggttgtttta aatcgcccgc cttcggcgca tagctgcggc tgccgttttt
      541 catccaatgc ttcaggtaag cgggttctgc ctgtttggaa ccgatcctgc ctttttgcca
      601 ttgggggtgg ctgttgacaa attcggcaaa ctcggcattg gtaacgggat atttatccag
      661 tttgaacggt ttgactttaa tcagaccggt atcttttttc agataaagcg ggcggtagct
      721 gccgccttcg atttgaacca tttcggcagc tgccgcttga gtgccggcga gtgccgcaca
      781 gagaaagagt aaccgtgcaa acttcatatc atgcctcctg acaggcagtt aaaatcaatc
      841 ttccgaaagg aaagattggt tgttaaaaaa ccactgccgt gcgtaatgag gtacggcggc
      901 agtggtttgt cccgctaatg acgggtatcc gatttaataa acgctttttt cggattcaga
      961 ggctgccggg gcagaagctg cgggagcgga agctgcggaa gccgcaccgt taccggcgta
     1021 agcggtatca ctcaattttt gagtcatgat ttcagggttt tccgcaccct ctactttcaa
     1081 ttgccccaac gcgcctttgt tgaatgcgcg gaagatagag tggtcaacca aagtgtagct
     1141 gcccgggatg tcgactttga attcgacgat ggcagagccg ccggcaggaa cgatggtgct
     1201 ttgtacgttt tcgttaatca gtttgccgcc ttcaacataa actttgtcga agatttcgcc
     1261 gatgacgtgg aaggaagata ccaagttcgg accgccgtta ccaacgtaca tacgtacagt
     1321 ttcgcctgct ttggctttca gcgcgttatc gccggcgata gcacctacgt gaccgttgaa
     1381 tacgacgtat tcaggctgtt cggcaacggc tttgtccata tcgaacggtt gcagaccttg
     1441 cgcgcctttt ttgcctttgg tgtagaagtc gccttggacg atgtagaact ctttatccac
     1501 tttcggcagg ccttctttag gctcgaccaa aatcaggccg tacataccgt tggcgatgtg
     1561 cataccgacc ggcgcgacgg cgcagtggta gatgtacaga cccggttgca gggctttgaa
     1621 gctgaatgtg gaagtacggc ccggagcggt aaaggttgcg gctgcaccgc cgccctgacc
     1681 ggttgcggcg tggaagtcga cgttgtgcgg aacggtagaa gaaggattgt tggaaaattc
     1741 cacttcaacc gtatcgcctt cgcgtacgcg gatcatgcga cccggaacgt cgccgtcaaa
     1801 tgtccagtag cggtattcca caccgtcgtc cattttcatg gttttttcga cggtttccat
     1861 tttcacgcgt actttggcag ggtagtcgcg gtcgattgca ggaggtactt ctggagcgtg
     1921 ggtggtaact gcatcgatga cgggcagttc gcctgcaggc gtttcggcgg cggtttgtgc
     1981 ggcggagctt gcggcttcgg cggaagcggc aggggtttgc gcggcaggtt cgccaccgca
     2041 ggcggctaaa gcaaacatag aagcgataat tgcggctaag gcttggcgtt tcataatgtt
     2101 ttccttttgt aagaaaagta gggagattgg ttaagaactg gtgtgattat gcgtttgccc
     2161 cattgggggc attttgattt aagtcaaata attcataaaa tatgaactta aaatgatact
     2221 tctaatactt tatagctact aaaaattaca ataaataggc tgatttgctt aagggcttcg
     2281 gcaaatcagg caaaggcagg gtagttgccg actaaagaat aatgatagtt attatcatat
     2341 atgtatttgt tttatatgtg agtttctttg aacaaatgtt tatttgcgcg gtaaaccgtg
     2401 ctacaatctt taacattcat attttgtgaa ttttaatcca ctatattaaa aggagctctc
     2461 aaaatgggac agtacaagaa gctgtggtac ttgctgtttg ccgttctggc ggtatgcttt
     2521 accattcttg gctatatggg cagcgaggtt tataagaaag ccccgcctta ccccgaacag
     2581 gtcgtttccg catccggcaa agtcttgatg acgaaagacg atattttggc aggtcagtct
     2641 gcgtggcaga gtaccggcgg catggaagtc ggttcgattt tgggtcacgg cgcatatcag
     2701 gctccggact ggacggccga ctggctgcat cgtgagttgt ccgcctggtt ggatttgacc
     2761 gcgcaacaga cttacggcaa aaaattcgat gaagtttccc ctgaagaaca agccgttctg
     2821 aaaacccgac tggctgacga ataccgcaac caaagccgta ttaaagaaga cggcagcgtc
     2881 gtcatcagcg atacgcgcgt gaaagccatc gaaagcatcc tgccttatta ccacggtgtt
     2941 tacggcgatg atcccgcgct gcaaaccacc cgcgaacact ttgcaatgaa aaacaacaca
     3001 ttgccaagcc aagaagcgcg tgaaaaactg ttcgacttct tcttctggac ttcttggtct
     3061 gcttcgacca accgtcctga cgagacgttc acttatacca acaactggcc gcacgagcct
     3121 ttgatcaaca acgtaccgac cactgaaaac tatatgtggt cattcaccag cgttgttctt
     3181 cttctgatgg gcatcggttt gctgatgtgg ggttattcct tcctgaccaa acacgaggaa
     3241 gtggaagtgc cgtctgaaga cccgatttcc aaaatccaac tgaccccttc gcaaaaagca
     3301 ttgggcaaat acgtcttcct gacggtcgcc ctgtttgtgg tacaagtatt gctgggcggc
     3361 ctgaccgcgc actacaccgt cgaaggtcag ggtttctacg gcatcgatga ggcgttgggc
     3421 tttgaaatgt ccgactggtt tccctatgcc ctgacccgta cttggcacat ccaatccgcc
     3481 atcttctgga ttgcaaccgg ctttttgacg gcaggtttgt tcctcgcgcc gattgtcaac
     3541 ggcggcaaag atcccaagtt ccaacgtgca ggcgtgaact tcctctatat cgccctcttc
     3601 atcgtggtcg gcggttctta cgcgggcaac ttcttcgcgt tgacgcacat ccttcccccg
     3661 gaatttaact tctggttcgg acaccaaggt tacgaatacc tcgatttggg acgtttctgg
     3721 caactccttt tgatggtcgg tctgctgtta tggctgttcc tgatgttgcg ttgcacggtt
     3781 tccgccttta aagaaaaagg cgtggacaaa aacctgttgg ctatcttcgt agcttctatg
     3841 gtcggcgtgg gcgtgttcta cgcgccgggt ctgttctacg gtgaaaaatc cccgattgcc
     3901 gttatggaat actggcgttg gtgggtggtt cacctgtggg tagaaggctt cttcgaagta
     3961 tttgccactg ccgcctttgc ctttgtcttc tacaatatgg gctttgtccg ccgcagtacc
     4021 gccactgcct ccactttggc cgctgccgcc atctttatgt tgggcggcgt tccgggtacg
     4081 ctgcaccacc tgtacttctc cggctctacc tccgcctcta tggcaatcgg tgcctgcttc
     4141 tccgctttgg aagtcgtgcc gctggtgttg ctgggccgtg aagcatacga acactggtct
     4201 taccaacacc tgtccgactg ggcgaaacgc ctgcgttggc cgctgatgtg cttcgtggca
     4261 gtcgcctttt ggaatatgat cggtgccggc gtattcggct tcctgatcaa tccgccgatt
     4321 tccctgttct atatccaagg tttgaacact tctgccgttc acgcgcacgc cgccttgttt
     4381 ggtgtgtacg gtttcttggc attgggcttc gtattgctgg ttgcccgtta tttgaaaccg
     4441 aacgcgcagt ttgacgacaa actgatgact tggggcttct ggctgctcaa cggcggcttg
     4501 gtcggtatga ttgccatcag cctgctgcct gtcggcgcga ttcaggcata cgcctccatc
     4561 acgcacggtc tgtggtatgc ccgcagcgaa gagttcctgc aaatggaaat cctcgatacc
     4621 ttgcgttggg tacgtacggc agctgacttg gtctttatcg gcggtgcaat ctgcgttgcc
     4681 atccaagcca ctaaaatcgt attcggccgc gacaaataag ttttgcggta tgaaatgagc
     4741 aactgcaccc gttttcgggt gcagtttttt attgcggatt gggacgggaa tggaaaagga
     4801 tgtggatttt ttattttaat cggctataca attgctgctt tcatgccgtc tgaagcacgt
     4861 ttcagatggc atcggtttac atcagataag gataaaaatg ggtatttacg attttcaaat
     4921 gaaagatgcg gaaggcaatg cggttgattt gtcaggctat cgcggcaagg ttctgctgat
     4981 cgtcaacacg gcaacgcgtt gcggtctgac cccgcaatac gaggctttgc agaagctgta
     5041 tgcacaatat accgcagaag gcttggagat tttggatttt ccgtgcaacc agttccgcga
     5101 acaggctccc gaaagcagcg gagaaattgc ccaagtgtgt atgatgaagt ttggcacgaa
     5161 gtttaagatt ttcgacaaaa tcgaagtcaa tggagcaaat accgcgccct tgtatgccta
     5221 cctgaaatcc gtcaaaccgc aagataaagg caatcatctg tttaaagatt tcgtgttgaa
     5281 gctcgcagct ttgggagaga agcgcgacga aggcgacatc aaatggaact ttaccaaatt
     5341 cctcgtcaac cgcgacggcg aagtggtcga gcgttttgcg cctagtgtta cgcccgaaga
     5401 aatcgaagcc gatattcggg cattgctgta atctgccgcc gtcttgggaa atgccgtctg
     5461 aaggcttcag acggcatttg tccgtttgcg ggtttattcc gaaatatcgg caacaccgct
     5521 ttcggggaag atgggcaaat ccggcggtgg gagcagggta tccaaagtca gggcggacgc
     5581 atcgagtgtg gggtagcctt caaacgcgac cgaataaccg ctgtttccgt tcatacggat
     5641 tcgggcgtgc gcgcccagcg ggaaagtgat tttgtcggca atgtgtccga acgggaagcc
     5701 cgtcagcacg gggattttcg ccgtgcgcga aacatggttg gcaacggcgg aaaaatcata
     5761 agacgaatca tagacatctc gaattttttc catacggaaa ttgccgaaca cgatggcgcg
     5821 ctgtttcttc aaaatacccg aaagatacag cgtattgagc atacgttcga tgcggtaggg
     5881 ctgttcgccg atatcttcga ggaacaaaat gccgccgtcg atgtcgggca tataaggcgt
     5941 accggcgagc gaggcgagga cgcttaagtt gccgccccac aacgtgcctt cggtttcgac
     6001 atcggcgcgt tggatataag gaacatcaac ggtcaggcgg ttttgggttg cccctttgat
     6061 aaaggcatcc atcgtaaacg caccgggggc aggtttgcca aaatcgctat aagccatcgg
     6121 gccggcaaaa ctcatcatat tgccttttgc caacaatgcc agttggacgg cgcacacatc
     6181 gctgaatccg aaaaacagcg tgccgtgttc gcgcatcctt gcgccgagcg aagcaaaatc
     6241 gatatgcggc agaatccgcg ccgcaccgta accgccgcgc aagcccatca gcactttagg
     6301 cgtggcgacg cggccggaag cgacctcttg gaaatcggcg gcacgttgcg tgtccgtgcc
     6361 ggcaaaccgt tggaaacggc ggctgcccgc ctgttggttg gttacggtaa aaccgacatt
     6421 gtaaaggcgg gttaaggctg tgttgacgcg gttggtgtct tcggcaaatc ctgacgaagc
     6481 gacaacgcgc agaaggttgc cgtccgaact ttgacggcgt ggcgtttgga gaggcacggt
     6541 tcgggctttc acaacggaat gggaagaggg aaggggcgga acggatgtgg cggatgtgcc
     6601 gcaagcctga agcagccccg cgccggcagc ggcggtgcag gttttcagaa aacggcggcg
     6661 ggaggtcggt tcggtcataa tttccctttc atacggtttc agacggcatt ggcgggctta
     6721 aagcagcttt ttaagctgtt ccgtccaatc ttgcggcagg gtcataccgt gtttccggac
     6781 ggggtttgcc caaatcggcg cggggaaaga cgcatcgttt tcaaagcggg cgataatatg
     6841 ccaatgcagg tgcggcacga cattgcccaa gctggcgagg ttgatttttg ccggccggaa
     6901 cacttggcgc atagcggctt cgactttgta caccatttcc atcaattcgc cgcgttccgc
     6961 tgccgaaagg tcggtcattt cggcgatgtg cttacgccaa atgacgcggc agaatgcagg
     7021 cgaaccgctg tcgttatgga tggcgatgac gcggaggttg ggggtttgca gcaaaatatc
     7081 ttcatcttgt gccgtgcaga tggggcaggg ttgggcggtc atttttcctt attcctgaaa
     7141 gtgtttaaaa aacggtaggg taatttctg
//
LOCUS       DAWFIE010000094         6705 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWFIE010000094 DAWFIE010000000
VERSION     DAWFIE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.094"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..400)
                     /locus_tag="WGQ71_001824"
     CDS             complement(<1..400)
                     /locus_tag="WGQ71_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0707788.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKNTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYFGGQRKGKRGRGAAGKVAVFGLLKRNGKVYTVT
                     VPNTQTATLFPIIREQVKPDSIVYTDCVAMM"
     gene            467..769
                     /locus_tag="WGQ71_001825"
     CDS             467..769
                     /locus_tag="WGQ71_001825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707789.1"
                     /translation="MKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGFNGDI
                     GEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWEMER"
     gene            complement(851..2938)
                     /locus_tag="WGQ71_001826"
     CDS             complement(851..2938)
                     /locus_tag="WGQ71_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ0707790.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGVALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMGLIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(2941..4206)
                     /locus_tag="WGQ71_001827"
     CDS             complement(2941..4206)
                     /locus_tag="WGQ71_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ0707791.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(4251..4388)
                     /locus_tag="WGQ71_001828"
     CDS             complement(4251..4388)
                     /locus_tag="WGQ71_001828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707792.1"
                     /translation="MNTETIYAIVFCILAATISGLLVKFNVIKIETSINSKFMLLGIS
                     I"
     gene            complement(5429..5665)
                     /locus_tag="WGQ71_001829"
     CDS             complement(5429..5665)
                     /locus_tag="WGQ71_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ0707793.1"
                     /translation="MKELHTSELVEVSGGKFHIFAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGISKKW"
BASE COUNT         2134 a         1199 c         1163 g         2209 t
ORIGIN      
        1 acatcatagc tacacaatcc gtataaacaa tgctgtcagg tttcacttgt tcacggataa
       61 taggaaataa agtagcggtt tgagtattcg gtactgtaac cgtataaacc ttaccatttc
      121 gcttcaaaag accgaatacg gcgactttac cggcagcacc gcgaccgcgt ttgcctttgc
      181 gttgtccgcc aaaataactt tcatctgctt ctacttcgcc atcaaacatt tctaaatgtg
      241 ggctgttttg ataaataagt aatcgtaaac gatgaaaata ataggctgcg gtatttttat
      301 taacgcctac taactctgct gctgttcttg cagttacacc tgcgacaaat agctcaatga
      361 gtttattttg tttataccgg cttagacgac tttttctcat agggataatt ctaacttaat
      421 ttgaatttcc ctagttatct aggacagccc cttaaaaaat atccctttga aagattttga
      481 tgaagtttgt gaaaagtggg agaaatcctt taaagaaaat ggaaaagaaa ttgaatataa
      541 tcaaaaatat tcaagaattt atggtgaagt tcgtggagct ggctttaatg gcgatatcgg
      601 agaaaagcta catcgttgtg ccgtgatgga atgggctgaa aatgagaccg tgaaattagc
      661 ccaaaaatgg gaacaagagc agaaaaaaca acaaattcaa cagaaaaagg aaactgaaaa
      721 atcgccaaaa cacaaagcca gtcgtgatga ttgggaaatg gaacgttaaa ccgctatttt
      781 catgtattct attaaattat ttgaaaaaag acttcacttt ttgttaaaga aaaatgaagt
      841 cttgttctgt tcaacctaaa ttcagaattt tatctgccga ttgaattgtt tcttggcggt
      901 gtgcaaccat tattttcata ataccaagac tttttaggtt atggttaatt ttttgttcat
      961 tttctacatc taaatggcta cttgcttcgt ctaaaaatag aattttgggt cgtttataca
     1021 atgcacgagc caagataact ctctgctttt gtccacctga taagatattt cccatatcgc
     1081 caatcaaggt ctcatagccc attggcattt taagtatatc gtcatgtatt tgtgccattt
     1141 ttgcacattg ttcaatgagc cccatatttg ggctttcatc aaaaaatgaa atattttccc
     1201 caatagaacc tgcaaaaagg acatcatctt ggcgaacaat cccgctcaat cccctaataa
     1261 aggatggaga aacttgatat atatcatgcc cattaatact aactgtacca gtttcaggtt
     1321 ttaggctacc tgttaaaatg tttaacaaag tggacttccc ccgaccagat tgtcctgtta
     1381 aaacaactgc ttcattatct ttaaattcca aattaatgtt ttcaaaaaga tatggctcat
     1441 tatcagcata tctgaatgag acgttttcaa ctttaagaac cagttgttca ttatctaatt
     1501 taggtatatg attatactta ataatttcag tttctgtttc atttaaagta atgtcagcca
     1561 aacgttcagc atgaagccct aacattttga tttggatgta ttggtcaaca agagaagctg
     1621 ttctgctttc aaattgccct ttataagcca aaaaagccat cagaacaccg actgtaaatg
     1681 aaccatctaa aattgcgctt gcaccaagat aaattatgat aatattttcc atgctaaaca
     1741 acagtttatt tgaaaattca aataaagcag agagtttatc tgttgtcagc ttggtattga
     1801 ctgtattcac aaataggctc atccaagtgc catgtctttg ataatgttta tcaaataatt
     1861 taactgattg gataccacga atggtttcca tgaaatatga gttttgtttg gcttcatgaa
     1921 caatattttc ttctgttgca tttcttaatg ggtaatatgc aagccaacga attagtatgt
     1981 acaaaacaag tgttaaaaga acaatcagcg ataattgagt gctgtaaatt gtcattaaca
     2041 cgaaagtaaa aacagccatt aagctattta aaactaaaac aaaaaaagta gaagttagtg
     2101 tttcttggat atgatctatt gaaccaaatc ttgaaatcac atctcctaaa tgtcgtttac
     2161 tgaaatagtc attaggtaag tcaagtaacc ttttaaatat attggctttc cattgtaaat
     2221 taagagttgt agataggtgc atacctaccc atgcttgtaa caggctaatt aactgttgca
     2281 ggatagccag taaaccaaat cccaaagtaa gggtcagtaa taaattttta tcagcagtta
     2341 caatgacatg gtctattacc cattgcataa agaatggact aaccaatgca aagacttcca
     2401 aagaaatagc taatataagc atttgaatta aagagcgttt taagcctgaa atccccctta
     2461 atagagataa tattttgatt ttctttgttt cttttttctc ttcaaaatgg gtattgggga
     2521 ataattctag ggcaacccct gtgaattttt gtgaaacttc gtccattttg atttttcgca
     2581 taccgacagc agggtccata atgacgatac tgtctttgga aatggaacaa agtacaacaa
     2641 aatggtttaa gttccaatgg agaatgcagg gtagttgtaa atttgacagc tcatctaact
     2701 ctaaacgcaa agctcgtggc gttaaattca tttcattgcc aaatctcatg atgtctgcaa
     2761 gatttgcgcc ctttaatgac agggtgtatt tttggcgtaa cgttcttaaa tcagtgtgaa
     2821 agccataata ggacaagatg gatgtcaggc atgctaaacc acattcagca acttctgttt
     2881 gcagaatgac aggtagcttt ttgttaaatc caaaggacag tcttgataaa taatccattt
     2941 ttaattgatt tttcctgaaa tgctgtaaag tgggtcaagt acccattcgt acaatttttt
     3001 tcgttcatgg agaatatctg cttctaaaat catgccaatt tgaagcggct tgttttctcc
     3061 gtatgcttta atcgtttgtt tttccaattt aactttcaca agataggcag gttcatttaa
     3121 taaggttggg ttagtgaaaa tgatacctaa acctgatagc tcttgtttac cgagagcagt
     3181 tctggcaact gaaataattt ctcctgtggc atgtccaaat ttttggtaag ggtacgcttg
     3241 gtaacgtaaa acaactttat ctttcggttt aataaaacca acagctttac tgggtatgta
     3301 aagattggcg accaattcag tttgttcagg gacaatgctt aacagcaatt tagacggttc
     3361 aacttgttgc cctatatcaa cattaattgt tgatatataa cctgatttac tagctcgtat
     3421 ggtttgttcg gatttcaaat caaaatccaa aatttcttgg ttcatttccg taatcgcacg
     3481 gttgagttgg ctcaattcgg ttttatggcg ttcaggcagg ctgcttaatg tgattttctg
     3541 ttcatcaagt tccctgattg cattattttg ttcacgtttt aggctctcca aacgtgagcg
     3601 ttgttccaat aaatggcttt cggcggtcat cttatcttgt tgggatactg cgccttgact
     3661 ggctaaaaac ttgttcttgt taagggtttt ttccgctaaa cgaatttgac gattttgccc
     3721 tgtaatttgc tgtttaatat tctctaattg attgtttaaa cgatgaatgt tgttatgaac
     3781 atttttttgc tcattttgat gaatgcgctt taaacgttcc aattcttgta atgccaaagt
     3841 ttttttaagg ttggcttctg ctgccaattt ggcttgtacg tttccttttt cgccaaaacg
     3901 cgatgtggaa agtttgaaca atggttcgcc agctttgaca aagttaccat cttcaacaaa
     3961 tttatgcgta atcgtgccga tatcggaaga gtaaacacga accaccccca tagttggaag
     4021 taattgacct tcaacggttg ttttattggt atagctacca aaaatcaaaa agataatgat
     4081 acacagagca atgagaaaag cgcaaaaagt cagaaataaa aaagagaatg gacgggtcaa
     4141 gataacctga cctgtccact tattttgttg ggctacaaag acttcttttc ggaataagtg
     4201 ggacatttat tattatttct taaaaaaaat ggatagaaaa acaccaataa ttaaatactt
     4261 atgcctaata acataaattt gctattgatt gatgtttcta ttttaattac attaaattta
     4321 accaataatc cagaaatggt tgcagctaaa atgcaaaaga caatggcgta aatggtttca
     4381 gtattcataa tttttctctt tcaaaattgt tcaatctaaa tagaaaaagg taacttcaat
     4441 acatattgct gtaactgaaa ttaccttttc cttaatgatg taattatctt atggcttcat
     4501 catgccacga ccaatattgt atccgccagc agcagagaaa ataccagctc caattgcgcc
     4561 aacccagcct actggtcctg ttcacttgtt ccgttgagct acaaagactt ctggtctgaa
     4621 aaatgagtga ttgttagaca tgtgtactta tttatgtttc tttgattggg ataaatttgt
     4681 aaaaacaaaa aaaaggcaag ttgttattat agaagaactt aaaatgctta acatactcat
     4741 tgaggtttgt accacataat gagatacgag ataatttgtt gggagtaaaa tcaaaaaaaa
     4801 gattaaatat ttaaaataat cactatattt tttcattatt tgttcctttt tgtatttaag
     4861 ttcttatgaa aaactagggt attttgaata gaaaatatca aaatacccta gttttattaa
     4921 ggaatattac caccaacaag tgcttccata ttgtacagca ccagtgattg caccaccaat
     4981 agctccgaat ttcgcacctg gaatagcacc aataccacct gcaaatgaac caacaattgc
     5041 cccaccagct gctccaccta atgcactacc aatggtattt tttgagaaat cacgccagtt
     5101 acaagcagca ccagaaactt gttaaatttc attcaaagtc aaaacttgca taatattcac
     5161 ctttgttaag tgtggttgag aaaaaagtaa attttcatcg ttactcatta tttgcaatca
     5221 tgcatatact gtaaccaagc actatattca gttggatcat agtttttcct actgggttgt
     5281 ctgatatgac tattggcact attggcacaa ttgggattag caactttatt gtgagtacct
     5341 aatggattga tatttcctgt aaagtggcta ttgcatgcgc ttaatgagca tagatttatt
     5401 aatgtcagaa aggataaaac aaaaaatctt accatttttt actaatacca aaagtaggtt
     5461 tcgaaaactg tacaccaaga ccttgtacat atccaaattg cttgctaaca gaaaactcta
     5521 ctccactatt gttaggggaa taggaagcac caattttacc aacagcaacc atatcttttt
     5581 tacctaggtt gccgccaccc tgtgcaaaga tatggaattt gccacctgac acttcaacta
     5641 attcagaggt gtgtaactct ttcatgattt tctcctgtaa agaagtatgg ttggaaatgt
     5701 aaatcagaat taacctttct taagtttaat ttaatacttt ttttgcaagt attttatttt
     5761 aaattaaatc aatattttaa aaatatttac ttgtatactg aaaaagaaac tcctaatggt
     5821 ggagtggggc ttttgtttaa gtaagcatta aaaaaattta aatttcccat tatgattaac
     5881 ttaatgcaag agcttttgca ggacgtacaa accagcgaac gggcgttctg atgtgggcag
     5941 gttggttgtc gctaaccaat ttcagtagct caatcgtcag attggtatcc gaccctatca
     6001 agctaaggag aggctgcggc tactacacaa agcacctgaa attattgggt gcttttgtct
     6061 ttttatggca ttgtaccaat ttgtgtacca cgagggcttg ttcgcccaaa cgcggtaaca
     6121 actgaataca cactttcccc ctcaacttct ttgcgttcta aaacgactgt cccaatccga
     6181 gaatagacaa tcataggacg atgattgccc cttacatcac taaattcact aaaaattttt
     6241 gcgcctgatt gaaaaatcat ttccacataa aagggttaca cctgtttcat cgtggcttaa
     6301 tccaaaccgc gttaaatctg ctttgtgttc tgccaaaatg tgaatcacac caaagccgtg
     6361 attaaccttt ttgtgttcgc cgtgttttaa aacaatttga cctgccgaga agtctgctct
     6421 attgttcgga caataggggg aatataccaa ttactttaac tacatcattt tttgacattt
     6481 ttcttgcctt tcaatttaaa atccaaaatc atactgccat aatttagcaa tccaaaaaaa
     6541 tttaggcagc agtcatggtt tcgtatttta gaaaacgaaa cttggtttgg tgttttgaag
     6601 catttcagta cacaaacatg gaattttaaa ttgtcttgtg ttgccttgat ttaggatgtt
     6661 ccagtttttt aataaatagt ttggggctgt cctagataac tagga
//
LOCUS       DAWFIE010000095         6676 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWFIE010000095 DAWFIE010000000
VERSION     DAWFIE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.095"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(148..990)
                     /locus_tag="WGQ71_001830"
     CDS             complement(148..990)
                     /locus_tag="WGQ71_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0707794.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHVVLWKQALDEGVPYVAVFEDDVLLGEGAEKFLA
                     EDGWLKERFDPDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
     gene            complement(1032..1127)
                     /locus_tag="WGQ71_001831"
     CDS             complement(1032..1127)
                     /locus_tag="WGQ71_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ0707795.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILRRLLKNR"
     gene            complement(1139..1966)
                     /locus_tag="WGQ71_001832"
     CDS             complement(1139..1966)
                     /locus_tag="WGQ71_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0707796.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSSVEKACFMSHVVLWKQALDEGVPYVAVFEDDVLLGEGAEKFLD
                     EDAWLQERFDKDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAVLPSEGLHPVDLMMFSDFFDREGVPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            complement(2008..3054)
                     /locus_tag="WGQ71_001833"
     CDS             complement(2008..3054)
                     /locus_tag="WGQ71_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0707797.1"
                     /translation="MPSEAFRRHRAYRENKLQSLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLEILIVDDGSTDGTLAIAKDFQKRDSRIKILAQAQNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTLPAGAWLDFAADGRMRRLFTL
                     RQYFGILHRLLKNR"
     gene            complement(3071..5134)
                     /locus_tag="WGQ71_001834"
     CDS             complement(3071..5134)
                     /locus_tag="WGQ71_001834"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ0707798.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGGLLEDIINQVVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEQTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDVVAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            complement(5155..5565)
                     /locus_tag="WGQ71_001835"
     CDS             complement(5155..5565)
                     /locus_tag="WGQ71_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ0707799.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQALIFGVRGSLHQAA
                     DDAEAFDRFMDELIAYLEANGLEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            complement(5641..6546)
                     /gene="glyQ"
                     /locus_tag="WGQ71_001836"
     CDS             complement(5641..6546)
                     /gene="glyQ"
                     /locus_tag="WGQ71_001836"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0707800.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
BASE COUNT         1427 a         1781 c         1773 g         1695 t
ORIGIN      
        1 ccgtcattcc cgcgcaggcg ggaatctaga aatttaacgt tgcggtgatt tatcggaaat
       61 gactgaaact caacggacta gattcccgcc tgcgcgggaa tgacagtgta tccatttcta
      121 attttaatcc gctatatttt acacaaacta tttgaacgat atgacccgcc tgccgtaagc
      181 tttctcaagc tccgcctgcc tttgacgctc cattcttttc ttcttttctc taccgaattt
      241 acccaaagca cgcttcaagt caaacattac cttcaacgaa cggcggtgtc ttctttcttg
      301 ttccctatct ttttccaaat cgctacccaa catactgttt ttactgagga acttggcata
      361 atgcaattct tgggtacata aggcgggatt aacctgataa acaggcatcc cctccttatc
      421 aaagaaataa gtaaacatca tccaatctac cgctttaatc cactctgccg gcaaaacggc
      481 aaacctttcc aagaaaaacc gcatcgcctc acgcgaaatg atatagccag ccgtccccca
      541 atgttcgctc tccagcaaag gaaatgaccg gttctcataa ttcaggactt tatccggtct
      601 gacaataact ttcgcaaaca tcgtttccaa acggacgata aaggcggaat ccgggtcaaa
      661 ccgctctttt agccagccgt cttcggcaag gaatttctcc gccccttcgc cgagtaagac
      721 atcatcctca aatacggcaa catacggtac gccttcgtcc aacgcctgct tccacaatac
      781 gacgtggctc ataaagcagg ctttttccac ttcgctcaac aggtgctgtt ttgccaatcc
      841 cggcaccaat tccgccatca tccgattcag ttcttcagac ggcatcagtg cgtcgaaaaa
      901 ctgaaacgga atgtcgtgcc tgccgaaggt atcggcaatg tgcgccctgc gttctgcggc
      961 ggaagctaag ctgataacat ggttttgcat aatttatcct gttttttgtc tgttggataa
     1021 agcggcgttt ttcaacggtt tttcagcaat cggcgcaaaa tgccgaagta ttgcctcaag
     1081 gtaaacagcc gccgcatcct gccgtctgct gcaaatacga tgtccatctc tcctcctttt
     1141 attggaaagg cacaatgaac tgttcgcgcc tttgccggcg tttttccctt tccctgctga
     1201 ttttggtcaa ggcgcggatc aggcggcgtt tgaatgtgtt ggcgggggaa tcgcgccatt
     1261 gctgtttgcg gttcaggagg cggtcgtgtt cgatcaggct gcccaatgcg ctgttttggt
     1321 cgtgaaactt ggcataatgc agctcttggg cgcacaaggc gggattgagc tggcaaaccg
     1381 gcactccttc cctgtcgaaa aaatcgctga acatcatcag atcgacgggg tgcagccctt
     1441 cggacggcaa aacggcaaac ctgtccaaaa aaaaccgcat tgcctttcgg gaaatgatat
     1501 agcccgccgt cccccagtgt tcgctttcca acagcggaaa ggcacgcccg cagtagtccg
     1561 ccacaccgga gggcgaggtc aggacgtgca taaacatcgt ttccaagcgg acgataaagg
     1621 cggaatcctt gtcaaaacgc tcttgcagcc aagcgtcttc gtcaaggaat ttctccgccc
     1681 cttcgccgag taagacatca tcctcaaata cggcaacata cggtacgcct tcgtccaacg
     1741 cctgcttcca caatacgacg tggctcataa agcaggcttt ttccacacta ctcaaataag
     1801 gatgcgccga caagccgggg acgagttccg ccattgcctg ctccagcctt tcagacggca
     1861 tcagtgcgtc gaaaaactga aacgggatgc cgcgcgcgcc gaaggttgcg gcaatgtgcg
     1921 ccctgcgttc tgcggcggaa gctaagctga taacgtggtt ttgcataatt tatcctgttt
     1981 tttgtctgtt ggataaagcg gcgtttttca acggtttttc agcaatcggt gcaaaatgcc
     2041 gaagtattgc ctcaaggtaa acagccgccg catcctgccg tctgccgcaa aatccagcca
     2101 cgcgccggcg ggcagcgtgt ccgtccgttt gaagcattgg tacaaaaacc ggcgggcgcg
     2161 ttcaaaatct tcttccggca aatgtttctc cagcaattca tacgctactg cttttatttg
     2221 gcggtattca aggctgtcga accgggtttt aaaacccata gactgcaaaa aatcgtttct
     2281 ggcggttttt tggatgcctt gcgcgatttc gtgttggcgg atgctgtatt tggatgaaac
     2341 ctgattggcg tgaaggcggt atttgaccaa ggcttcggga taataagcca gcctgcccaa
     2401 tttgctgaca tcgtaccaaa attggtaatc ttccgcccaa tcccgctcgg tgttgtaacg
     2461 caaaccgccg tcaatgacgc tgcgcctcat aatcatcgtg ttgttgtgta tggggttgcc
     2521 gaaagggaaa aagtcggcaa tatcttcgtg tcgggtcggc tttttccaaa ttttgccgtg
     2581 cctgtgatgc cgcgccagcc ggttgccgtc cttttcttcc gacaaaactt ccagccacgc
     2641 gcccatcgcg atgatgctgc ggtctttttc catctcgccc acgattttct caatccagtc
     2701 gggggcggca atatcgtcgg catcggtgcg tgcaatatat tcccccattc ctgactttgc
     2761 caattcgtcc agcccgatgt ttaaagaggg aatcaggccg gaattttgag cttgtgcaag
     2821 gattttgata cggctgtccc gcttttgaaa atccttggca atggcaagcg taccgtctgt
     2881 cgagccgtca tcgacaatca aaatctccaa gttgcgccaa gtctgattca cgacggcggc
     2941 taatgattgg gcgaaatatt tttctacgtt gtaggcgcaa atcaatacgc tgactaaaga
     3001 ctgcaattta ttctcccgat aggcacgatg ccgtctgaag gcttcagacg gcatttggac
     3061 tgtacaacgg ttactcgccc aaaagcgcga tgtcggctac cgcgttcatc tgctctgcca
     3121 agcggttcag caggttcagg cggttttgtt tcacggcggc atcttcagcc atcaccatca
     3181 cgccgtcaaa gaaggcatcg acttgcggct tgacggaagc cagttcggac aaggcggttt
     3241 ggaaattgcc ttcggcgacg gcggcggcga ttttcggctg caagccttgc gcggcggcaa
     3301 agagggcttt ttcttcgtcc tgttgcagca ggctttcgtt aaccgcgccc aactcggcat
     3361 cggctttttt cagcagattt tgcacgcgtt tgttggcggc ggcgagcgcg gcggcttcgg
     3421 gcagttgttt gaacgcggca acggcttgca gtttcgccac tacgtcgtcc aaacggcgcg
     3481 gctgtttcgc caatacggcg gcaacgatgt cttgcggata atcgttttgc agcaacacgg
     3541 caaggcgcgc ctgcataaag tcggcggttt cagacggcgt ttgctcgttg agcaagcctt
     3601 tggggaagct gtcgaatgcc gtctgaatca gttcgttcac gtccaaaccg tactgcatca
     3661 gcatccgcaa aatacccaag gcggcgcggc gcagggcgta ggggtcttta tcgccggtcg
     3721 gaatcagacc gatgccccaa atgccgacca gggtttccag cttgtccgcc agtgccacgg
     3781 cggcggcaat tttgctttcg ggcagcttgt cgccggcaaa acgcggctga tagtgctgct
     3841 cgatggcttc ggcgatttct tcggtttcgc cgtccaaacg ggcatagtat ttgcccatgg
     3901 tgccttgcaa ttcagggaac tcgccgacca tttcggttac caagtcggct tttgccagac
     3961 gcgcggcgcg ttcggcggcg gcggcatctg cgcccaacgc tttagcgatg tgggcggcaa
     4021 tgctttgcag gcgttcgatg cgttcggctt gcgaaccgat tttgttgtga taaaccacgt
     4081 ttgccagctt gggcaggcgg ctttccaaag tcgccttttg gtcttgtttg tagaagaact
     4141 cggcatcaga caggcgcgcg cgcaaaacgc gttcgttgcc tcggatgatg tgcgacgggt
     4201 cttcggtttg caggttggaa accagcagga agcggttcat cagcttgccg ttttggtcga
     4261 gcagcggaaa gtatttttgg ttttgctgca tcgttaaaat caagcattcc tgcggcacgg
     4321 cgaggaaatg ttcctcaaaa ccggcttcca aaacaacagg ccattcgacc agcgcggtta
     4381 cttcgtccaa caatgcttca tcggcggcga cggcggcgtt cagactgcgc gcctgccctt
     4441 ccaatgccgt ctgaatcgcg gctttgcgct cggcaaacga agcgacgact ttgccttgcc
     4501 cgcgcatttg ttcggcgtag ctgtcggcgt tttcaatggt gatttcgccg ttggacaaga
     4561 agcggtgtcc caaggtttga ttgctgcttt gcagacccaa aacgctgacg tttacgatgt
     4621 cgccgccgtg cagcacaatc agcccgtgaa cagggcgcac gaaggtaaat gtgctgctgc
     4681 cccaacgcat cactttggga atcggcagtt tcttaaccac ttgattgata atgtcttcca
     4741 aaagtccgcc cagcggcttg ccggtttgga cgtattcgta ggcgtacaca tcctgcttgc
     4801 cgtcgtggac gatggtcaag tcttcgattt tcgcgcctgc accgcgtgcg aaaccttcca
     4861 aagccttggt cggcgcaccg tcttttacgg cattcgccac ggcagggcct tttttcacga
     4921 ttttttggtc ggcctgaacg gctttgacgt ttttgacttg aaccgccaaa cggcgcggcg
     4981 aggcataagc cgtaaattcg gctgcaccgt caaccagttg cgctttttcc aagccttcgg
     5041 caacggcggc ggcaaaatgg ttgcccagat tattcagggc tttgggcggg agttcttcgg
     5101 taaggagttc gattaaaagg gtttgggtca tcattcggct ttctttgaat ttggttaatc
     5161 tgcctgttta taggtttcgc tgtaattttc ccagccgtca tccccgtaaa aaccatcaac
     5221 cagcggggtg gcgtacaaag tggcgacatc ttcgcggcct gccagccaag agataatggc
     5281 ttttttctcg gtttcgccca agcttcgggc accggatttt tgaaacaggc acgaaaaatc
     5341 gccgcaatcg cccccgcccg ccatttccaa gccatttgcc tcaagatacg caatcagctc
     5401 gtccataaag cggtcgaacg cttcagcatc gtccgcagct tggtgcaaac tgcctcgaac
     5461 gccgaaaatc aatgcttgaa actcgcccaa atgcagcttt ttatgctggc ggcggttcat
     5521 tttgtgcagg cgtttcctgc tcggggtgcg gaaatagaca ggcataattt tcctaaaaaa
     5581 tataatggct tccggacggc tgccatatcg tgccgcccga acgtaaaaat cgtcgctccc
     5641 ctaggcggca tttgccttca ttaagggaaa gcccagtttt tcgcggcttt cgacatattt
     5701 ttgcgccacg gcgcggctca acgcgcggat gcgcccgatg taggtggcgc gttcggttac
     5761 ggaaatcgcg ccgcgcgcgt ccaagaggtt gaacgtatgc cccgctttga ggaccagctc
     5821 gtaggcaggc agggcgaggg cggcgttttc ttcggcaagc aggcgtttgg cttgcgcttc
     5881 gtagtcgttg aactggcgca gcagccaatc ggcatcgctg tattcgaagt tgtaggtgga
     5941 ttgttcgact tcgttttggt ggtacacgtc gccgtagctt acggtgttgc cgtccggcgt
     6001 ttttgcccaa acgaggtcat agacgttttc cacgccttgc aggtacatcg ccaagcgttc
     6061 gatgccgtag gtaatttcgc cgagtacagg cgtgcagtca atgccgccga cttgttggaa
     6121 ataggtaaac tgggttactt ccatgccgtt gagccagact tcccagccca aaccccatgc
     6181 accgagggtg gggttttccc agtcgtcttc gacaaagcgg atgtcgtgga ctttggggtc
     6241 gatgcccaat tcgcgcaggg agtcgagata gaggtcttgg atattggcgg gagcgggctt
     6301 gagggcgact tggaattggt aatagtgttg caggcggttg gggttgtcgc cgtagcgacc
     6361 gtctttgggg cggcggctgg gttggacgta ggcggcaaac caaggctcgg gcccgagtgc
     6421 gcgcaggcag gtggcggggt gggaagtacc tgcaccgact tccatgtcga agggttggat
     6481 aacggtgcag cctttgtctg cccagaatgt ttgcagtttg aagatgattt gttggaaggt
     6541 aagcatggct tatgattcga taaaataaag ggtttatttt actgtttcca ttgctgtttg
     6601 gataggttta tctcaaagac agactgattt gaaaacacgg catacatgat atagtggatt
     6661 aaatttaaac cagtac
//
LOCUS       DAWFIE010000096         6413 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWFIE010000096 DAWFIE010000000
VERSION     DAWFIE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.096"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            485..931
                     /gene="yciA"
                     /locus_tag="WGQ71_001837"
     CDS             485..931
                     /gene="yciA"
                     /locus_tag="WGQ71_001837"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ0707801.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKIEVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLPTP"
     gene            complement(982..1803)
                     /locus_tag="WGQ71_001838"
     CDS             complement(982..1803)
                     /locus_tag="WGQ71_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0707802.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKTAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1922..2380
                     /locus_tag="WGQ71_001839"
     CDS             1922..2380
                     /locus_tag="WGQ71_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0707803.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFADSSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2545..3660)
                     /gene="lst"
                     /locus_tag="WGQ71_001840"
     CDS             complement(2545..3660)
                     /gene="lst"
                     /locus_tag="WGQ71_001840"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ0707804.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKEKLFN
                     ERGKPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAEW
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTINLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKDFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            3761..3865
                     /locus_tag="WGQ71_001841"
                     /pseudo
     CDS             3761..3865
                     /locus_tag="WGQ71_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4093..6315
                     /locus_tag="WGQ71_001842"
     CDS             4093..6315
                     /locus_tag="WGQ71_001842"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ0707805.1"
                     /translation="MTKSTIIYTYTDEAPALATQSLLPIVQAFTRHADIDVKTSDISL
                     SGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQS
                     KGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKHPH
                     SMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALKA
                     GEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFFA
                     PVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVDS
                     DKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATIDF
                     CREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHDV
                     EAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLAE
                     LDTDGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKML
                     SIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGNA
                     KAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELKA
                     AFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNAL
                     "
BASE COUNT         1724 a         1870 c         1423 g         1396 t
ORIGIN      
        1 cccgagattc tagattccca cttccgtggg aatgacgtgg tgcaggtttc cgtatggatg
       61 gattcgtcat tcccgcgcag gcgggaatct agacctgtcc gcacagaaac ttatcggata
      121 aaaacagttg ctcaaacccc tcgttctaga ttcccacttt cgtgggaatg acggttcagt
      181 tgctacggcc ccgccctaca atacgccttg cgaatctgtc ccgcgcccgc ccttatatgt
      241 ttcaaatgag ttaaactacg cccttcccga cttcccgttt cgggcagggg tacaggtccg
      301 atttatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacagatag
      361 tacggaaccg attcactcgg tgcttcagca ccttagagaa tcgttctctt tgagctaagg
      421 cgaggtaacg ccgtactggt ttaaatttaa tccactatat atttcaataa aacaaggaaa
      481 ctttatgcag cacgaagaag gcaaccgcca acgccctcaa ggcgaactgc tcctgcgtac
      541 cgtcgctatg ccgcgcgata ccaatcccaa ccaagacatt ttcggcggct ggattatgtc
      601 gcagatggat ttgggcggcg gcatattggc ggcggaaatc gcgcggggac gcatcgttac
      661 cgtcgccgtt caggaaatga acttcatccg cccggtcaag gtcggcaacg tcgtctgctg
      721 ctacgggcat tgcgtccgcg tgggcaatac ttccctccag cttaaaatcg aagtctgggt
      781 gaaaactttg atgaacgatt gcgttaccga agaccgctac ctcgtaaccg aggcggtgtt
      841 cacttatgtt gccatcgatg cggaaggcaa tccgcgcccg attccgaaag aaggcaaccc
      901 caaattgtcg ggcttattgc ctactccgta aaaatacccg taaaaatgcc gtctgaaact
      961 gtcttcagac ggcattccgc atcatttgaa ccaggacctg atgcgggaaa acagcgacat
     1021 actctgtttt tcaaagtctt cccgcacttc gggcacggct gtttttatga ccttcattcc
     1081 tgcaaaagtc tgcgccacgg gcataatttc aatcgtgttc acattcatat gcgccggccg
     1141 gcggtacagc cacaatgcgg tttccgcaat atcttcgggg cggataaatt ccacaccctc
     1201 atacacgcct gctgccttct cgtcatcgcc tttgaagcgc acgttggaaa actccgtatt
     1261 gccgcacaaa cccggctcga tattggtaac gcggatgttc ttatccgcca actccgcgcg
     1321 caaattcagg ctgaactggc gcacaaacgc cttggtcgcc ccgtaaacgt tgctgccggc
     1381 ataagcataa ttgcctgcaa tcgaacccaa attcatcaca taaccgccgc cgcgttccac
     1441 catttgcggc aaaattttgc gcgtcaggaa cgtcaaaccc aaaacattgg tttgaatcat
     1501 cgtttcccaa tcttcaaaat ccgccttgtc cgccgtgtcc agacccaaag ccagcccggc
     1561 attgttgatg aggcagtcga tgtcggaaaa ttcatcgggg atgccgttta aggcgttttc
     1621 caccgactcg cgtcgcgaca cgtccatttc caaagggtaa aacaaagcac ccaattcatc
     1681 cgccaaggcc tgaagcctgt ccgcacggcg cgccgcaccg ataacgcggt atcccgcccc
     1741 gacaaacgca cggcacatcg cttcgccgaa tcctgccgaa gcaccggtaa ttaaaacagc
     1801 cattgttttt cctttctttc tcgtttccca aacctgtttc aggtatcata acgctcttca
     1861 gacggcatcg cgccggctga aaatgacaac aaccatacca ccacgacagg acacattcaa
     1921 tatgaaaacc caaatcagcc ttgctgctgc cgccatcaca ctgctccttt ccgcctgcgg
     1981 aggcagcgga ataccttccc aacccaaagg cgaaatttcc gaaaaccgca ccgccgcgtt
     2041 caaatctatg atgcccgact tctcgcgtat gggcaaaatg gtcaaaggcg aagagcctta
     2101 cgatgtcgaa aaattcaaac aggcggcagc ggcgtttgcc gacagcagca aaaaaccgtt
     2161 tacgcttttt gagtccgacc cgcaaggcaa cgggcgcgca ctgcccgccg tttggtcgga
     2221 tggtgcaaaa tttgaagccg aaaaaacaaa attcgccgcc gccgtcgaaa aactcaacgc
     2281 cgccgcccaa accggcaaac tggacgaaat caaagccgcc tacggcgaaa ccggcgcaag
     2341 ctgcaaatcc tgccacgaca gtttccgcgc gcccgaataa catccaaaac aaaacaatgc
     2401 cgtctgaaga cttcagacgg cattttcttt gccatttcag cacacgcaaa gcaatcagaa
     2461 attatcgtca atccaaatga aaaaacgcta tccttatttt taccccacgc actccgccat
     2521 tttctatttt gatttgctat gcgattaatc tttatcgtca aatgtcaaaa tcgggatgcc
     2581 ggattgggta aacagggcat acaccggctt gagccaataa tcttctggaa gggaagccgg
     2641 tttcaatgcg taaacgtgca cattgggaaa atccttcatc gtcaacgccg cgccgctgaa
     2701 aaaggtataa atttcatacc tcgtatgcgg gtttttctta atttcgcgca aaatatagtc
     2761 ttcgatgaca tagggcgaat ttaatgtggt tacgccggaa agcccgtagg tttggcgagg
     2821 gtgcggcgcg acatattgta tgttgaaatc ttttgccgcc ttttccgaaa tttccttcat
     2881 ctccttgtcg ggcgaaccca aaagtatccg caccgtgccg cctgtttcgt cccccgcctt
     2941 cagttcggac gcatcgaaca gcggcaggta agtcatcttg cggcggccgt cgtccataat
     3001 gtttttcaaa cccttgaata tcgtgtaatg ctcgtcggaa gcattgcggg ttttggcgat
     3061 gctccaatct ccgatcatca tccgggcaaa attccgcttg atcgtcccgt ttacagaaaa
     3121 ctcatcgccc aaatagctgc tgctttgaat taaattgatt gtcccgtcgt caaaggtttt
     3181 gatttccgca tccgggtaag tgctcaaaaa ggcggcaatg ctgacttttt ccaaacttgc
     3241 caaataaatc cgcttgacct tcggcagcag catcgacttt accttcagct ccgccatcgt
     3301 cggaatgaaa ttaaacgatt tgttcaaacc gtagggcagg tggaaaaaat acgcccactc
     3361 cgccttatcc tttatctgat tgaaataata atcgtatttt tcattcctgt tttcagacat
     3421 cagcaccaca taaaaccgct cccccggatg ctgcgccata atcctttccg ccaccttcat
     3481 ctgcaatatg gtataacaga aaatcagatt gaccggtttc cccctttcat tgaaaagttt
     3541 ctccttcagc agggaaaccg cattcctttc cccctgattt acccggtcaa atgtataaaa
     3601 tatcccgaaa caaaaaacaa tcaggcacaa cacggtcaaa caagcctttt tcaagcccat
     3661 atccctaaaa ctccattccg acaaattaaa catacgcccc gagtgtcccg ggacggaaaa
     3721 atgcggaatt ttagcagata ttccgacagt taccgaatat tatagtggtt taaatttaaa
     3781 tcaggacaag gcgacgaagc cgcagacagt acaagcagta cgacaaggca aggcaacgct
     3841 gtactggttt aaatttaaac cactataaaa tcaatttcac cgaaaatatg ccgccgcaca
     3901 tcaggcacag cacctccgac cgccgccaaa cgcgggaaaa ccatttctcc cttcctttca
     3961 cgccgccgcg ttcaaacacg ccaagatcga acaaagcgcg aacaaagccg ccaaaatatg
     4021 ccataatagc cgcgcacatt ccccgcgcaa tgtgttccaa aagaaaaaac caacctacaa
     4081 gagaataatc ccatgactaa atccaccatt atctatacct acaccgacga agctcctgca
     4141 ttggccactc aatccctgtt gccaatcgta caggcgttta cccgccatgc cgatattgat
     4201 gtcaaaacca gcgacatctc tctctccggc cgtattttgg cggcgtttcc agaatacctg
     4261 accgaagcgc aacgcgtacc tgatgcgctt gccgaattgg gcgaactggt gaaacaaccc
     4321 gatgcaaacg taatcaaact gccgaacatc agcgcgtccg tacctcaact gactgccgcg
     4381 attaaagaat tacagtctaa aggctttgcc gttcccgact atcctgctga ccctcaaacc
     4441 gatgaagaaa aagccgtacg cgaacgctac gaccgcatca aaggcagcgc ggtaaaccct
     4501 gtcctgcgtg aaggcaactc cgaccgtcgc gcccctaaag cagtgaaaaa ctttgcgaaa
     4561 aaacatccgc acagcatggg cgcatggacg aaagactcaa aaacccacgt tgccaccatg
     4621 caaagcggcg actttttcca taacgaacaa tccgttaccg tacctgaagc gacttccgta
     4681 tccatcgtat tcaccgacaa acaaggcaac aaaaaagagc tgcgcgcgcc cgtcgccctg
     4741 aaagccggcg aaatcatcga cgcgaccgtg atgagcaaaa aagccctgct cgccttcctt
     4801 gccgaacaag tgaaagacgc gaaagcaaaa ggcgtattgt tctcgctgca tatgaaagcc
     4861 acgatgatga aagtgtccga cccgattatc ttcggacacg ccgtcaaagt gttctttgcg
     4921 cctgtttttg aaaaattcgg cgacaaattg gctgccgccg gcgtcaacgt caacaacggc
     4981 ttcggcaacc tgcttgccaa tttggacaaa ctggatgcgg acacccgcgc agccgttgaa
     5041 gctgaaatcg ccgccgttta cgccgccaac cccgatttgg cgatggttga ttccgacaaa
     5101 ggcatcacca acctgcacgt tcccagcgat gtcatcgtcg atgcctctat gcctgcaatg
     5161 attcgcaact ccggccgtat gtgggataaa gacggcaaag cgcaagacac caaagccgtg
     5221 attcccgaca gtagctacgc aggcgtttat caagcaacca tcgacttctg ccgcgaacac
     5281 ggcgcattcg acccgacaac catgggtacc gttcctaacg tcggtctgat ggcgcaagca
     5341 gccgaagaat acggttcaca caacaaaact ttcgaaatcg aagccgacgg ccaagttcaa
     5401 gtcattgatg cagcgggcaa agtcctgatg cagcacgatg ttgaagcagg cgacatctgg
     5461 cgtatgtgtc aaaccaaaga cgcgcccgtc aaagactggg tacaactcgc cgtcaaccgc
     5521 gcccgtctga gcaatacacc agccgtattc tggctcgatg aaaaccgtcc gcacgacaag
     5581 agcctgctcg ccaaggttaa agcctacctt gccgaactgg ataccgacgg cctcgacatc
     5641 cgcgtcctcg ctcccgaaga agccgctaaa ttcagcttag gccgtctgaa aaacggcgaa
     5701 gacaccatct ccgtaaccgg caacgtcctg cgcgactacc tgaccgactt gttcccgatt
     5761 ttggaactgg gtaccagcgc gaaaatgctg tctatcgtcc cattgatgaa cggcggcggt
     5821 atgtttgaaa ccggcgcggg cggttctgca ccgaaacacg ttcaacaatt cctcgaagaa
     5881 aaccacttgc gttgggactc tttgggcgaa ttcctcgcac tcgccgtatc gtttgaacat
     5941 ttggcgcaaa aaaccggcaa cgccaaagcc caagtcctcg ccgacacttt ggatgcagcc
     6001 accgaaaaac tgctgttgaa cgacaaatcg cctaaacgca aagcaggcga actcgacaac
     6061 cgcggcagcc atttctacct taccctctac tgggcgcaag aattggcggc gcaagacaaa
     6121 gatgccgaac tgaaagccgc atttacgcca ttggcagccg ctttgaccgc cgacgaagcg
     6181 aaaatcgttg ccgagctttc cgccgtacaa ggcaaagcgg ccgacatcgg cggctactac
     6241 gccgccaatc ctgaaaaagc cgcacaagtg atgcgtccga gcgtaacctt taatcaggca
     6301 ctaaacgcct tataagcaca aaggtaaaaa tgccgtctga acattcgtcg ttcagacggc
     6361 attagttatc cctatctgcc tgattatgat taaacgccgc acccattgga tat
//
LOCUS       DAWFIE010000097         6200 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWFIE010000097 DAWFIE010000000
VERSION     DAWFIE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.097"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(48..1559)
                     /gene="ubiB"
                     /locus_tag="WGQ71_001843"
     CDS             complement(48..1559)
                     /gene="ubiB"
                     /locus_tag="WGQ71_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ0707806.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGKPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKINALVDEARRQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
     gene            complement(1594..2058)
                     /locus_tag="WGQ71_001844"
     CDS             complement(1594..2058)
                     /locus_tag="WGQ71_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0707807.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPSRNIKLLVFGLLIAFLGYLLGKIF"
     gene            complement(2055..2201)
                     /locus_tag="WGQ71_001845"
     CDS             complement(2055..2201)
                     /locus_tag="WGQ71_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707808.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(2218..2556)
                     /gene="erpA"
                     /locus_tag="WGQ71_001846"
     CDS             complement(2218..2556)
                     /gene="erpA"
                     /locus_tag="WGQ71_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ0707809.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(2688..3374)
                     /locus_tag="WGQ71_001847"
     CDS             complement(2688..3374)
                     /locus_tag="WGQ71_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0707810.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3560..3832
                     /locus_tag="WGQ71_001848"
     CDS             3560..3832
                     /locus_tag="WGQ71_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707811.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            4040..5968
                     /gene="dnaK"
                     /locus_tag="WGQ71_001849"
     CDS             4040..5968
                     /gene="dnaK"
                     /locus_tag="WGQ71_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ0707812.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKAFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKTAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
BASE COUNT         1773 a         1640 c         1372 g         1415 t
ORIGIN      
        1 cggtttcctt ataaacgggc ggagtcgggc gatgattcgg acaaagccta tttcaaaagc
       61 aaaatcagca gcaaaacaac cgcaatcaca gccagccaca ggctttgccg ctgctgcact
      121 ttgaccaaat ggatataggc atcgcgcatt tcctgccggc gcgcctcatc gaccaaggca
      181 ttgattttgc gtggcaatga aggaatgatt tgcgcccagt cgggggcttc gtttttgagg
      241 ttgcgccaaa gggctttggg accgacctgc ccgttcatcc atttcaccaa aaacggtttg
      301 gcggttttcc acaagtccaa atcaggatcg agttggcggc ccaggccttc gatgttgagc
      361 agcgtttttt gcagcagcac aagctgcggt tggatttcga cattgaagcg gcggctgact
      421 tcaaacaggc gcatcagcac caagccgaag gaaatctgcg aaatcggttt gttgaacacc
      481 ggttcgcaca cggcgcggac ggcggcttcc aattcttccg cacgcgtgtc ggcaggcacc
      541 cagcccgatt cgatgtgggc ggtggcgacg cgccggtaat cgcggttgaa aaaggcgagg
      601 aaattgatgg cgagataacg tttgtcgtaa tcggtcagcg tgccgacgat gccgaaatcg
      661 agggcgatgt agcggttgtc ggcggcaacc aaaatattgc cggggtgcat atccgcgtgg
      721 aaaaaaccgt cgcggaagac ctgtgtgaaa aagatttcca cgccgtaatc ggcgagtttg
      781 tgcaaatcga tgccgtctgc tttgagtttg gcgatgtcgg aaaccggctt gccgtccatc
      841 cattcgattg tcagcacgtc gctggtgcag tagtcgtaaa acaccttggg cacaatcagc
      901 atattgctgt tttggaaatt gcgtccgagc tgtccggcat tggcggcttc gcgcatcaaa
      961 tccaactcgt cgtgcagata tttgtcgaac tccgccacca cttcgagcgg cttcagacgc
     1021 ctgccgtctg aaaacaagcg ttcgacccag gccgcgccga aacgcatcag cgacaaatcc
     1081 tgttcgataa ggggcaaaag gttggggcgc aaaactttaa ccgccactcg ttcgcccgaa
     1141 tgcaggcggg ctttgtgtac ttgggcgatg gacgcgctgg cgatgggctc ggtttcaaat
     1201 tccgcataca gcttttcgat ggactgaccc aacgattttt cgatttgttc acgcgaaagc
     1261 cgcgcgtcaa aaggcggcac tttgtcttgc agctttgcca gttcgacggc gtaatcgtgc
     1321 ggaatcaaat cggggcgtgt ggacaaaacc tgcccgaact tgatgaaaat cggccccagg
     1381 ctttccaagg caaggcgcag acggacagca ggcggttcgt ttttcaattt ggacgactgc
     1441 ggcatcattt tcagcagagc gcatatccaa ccgctaccca tcagcgaagc acacagacct
     1501 gccagccgat agcggtaaaa agtcccgaca ataaccgtca ggcgtttcaa ccatttcata
     1561 tataaaatcc cgaatacagc atttatcgga caatcaaaaa atcttaccga gcagatagcc
     1621 caaaaaggca atcagcagcc cgaaaaccag cagtttgata ttgcgggacg gcggttcggg
     1681 ttgcgaaggt gcggaaatat catcttcccg cccgccgtga agttcaagat attcgggcat
     1741 ttccccatcc gcgccttctc cttcgccctc cccttcctcc ggcggcacga ccgtttccaa
     1801 ctccatatat ctccgggtaa taatatccaa acccaaatag ctgaaataat catgcaactc
     1861 ttcccgtttc gcccaagtgc atttttcctc aaccagcact tgaggtttgg aatcgagcga
     1921 ttcgatcaat ccttccgcct tttcctcgcc gagattctcc ctgaggcact cccggataag
     1981 cccgcgatcc acttggggcg ggtaggaaac atatacatcg tacaaatcat ttcggtaatc
     2041 tttactcatc tcaatcataa gggaacaaat acgcctgata ccgttccgcc cgttcctttc
     2101 gggaaacccg attcatcttc aaacaatcgg attatatgtg aattcatccc gataatatat
     2161 tttccaaaag taaaaatgcc gtctgaacgt tcagacggca tcacacaaac caagcggtca
     2221 tacggaaaac gacgatccgc aaccgcaggt tgtttccgca ttcggattgc ggatgacgaa
     2281 ttgcgaaccc tgcaaacttt ccgtatagtc gatttccgca ccgaccagat attgatagct
     2341 catcggatcg accaaaaaga ccaaaccgtt tttctcaatt tcaaaatcgt cgtcgttttt
     2401 gatttcgtca aaagtaaatc cgtactggaa acccgaacag ccgccgccat tgacaaaaac
     2461 ccgcaatttc aaatcgggat tgttttcttc ggcaatcaaa tcggcaactt tggcacagca
     2521 gctgtcagta aaaataatag ggctttcatc cgacatggtg ttattcctta atataatatt
     2581 ttctgcattc tctctcaaac cccctttaaa ggcaagcagg cttcgccttt gaaaaaggaa
     2641 ttgttccaat agataagttt gaccgcctgc aatttcaagc cccaaactca aatcgtcctg
     2701 ccgaaccact cgacacgccc gataatctcc acatcatcgg tcaaatcgtt caaattgatt
     2761 tcaaaagcag gataagcctc gtttgcagaa atcacattga taatcccgcc cggtacaatc
     2821 tgtaaacgtt taaccagcag attttcatta atccgcaaca catacagacc gtccctcggc
     2881 gtattttcac catgattgac caaaatcgaa tcgccgtcat tcaaaacccc ctccatcgaa
     2941 tcccccttga cggaaattac agacaggttt ttcgtatcgc gggtaacata attctcaatc
     3001 caatgccgtc tgaacgccat tgtaaatacc ggttcctcat gatcgacaaa ctgcccgtat
     3061 cccgcagccg cccgaatatc atatctcggc acaaagacaa actcgtccgt atcgacttca
     3121 ttgcccaagg tatcgtaagc aagggatttt tttggggctt catccggaaa cggattaccc
     3181 tccccggtca gcagccaatc gatactacac cccttcaact gcttgatttt tttcaatgtt
     3241 tcagacttcg gcagaccgcc ttcattccat atcctgctga agcccgcaat cgtcatttca
     3301 atatcggatg cgatttttgc ctgcttcgct tcgcttttcc ataaaaaaac cagtctgtct
     3361 ttaaaggttt ccataaatat ccttccgctc cccttcattc aatcatctaa agtaaacatg
     3421 aaatatattt taccttagct atcccaaaaa cggaacaact atgataaact aaatttaaat
     3481 tatgattttt cttactaaat acataatttt taagattttc cataggttta atcctgaatt
     3541 gacagaaaaa ggatagaaaa tgaagaaaac cggcaaatat cttatctatg ctgcggcatt
     3601 taccgcattc tgctttgcct tccaagaaaa ccgttctgaa gccaaacagc ctgacatcac
     3661 tttatccgca tccctgtgcg aacaattcaa catactgaac gccaaagata tggatgcaga
     3721 acaagtctcc ctttccaaag aatgcgacat catcgagtct tcacacgact gggaaaaaga
     3781 gtacggcaac ttgaacgaac aggaaatgct cgccggcatc gtctatgaat aaacctgcct
     3841 gccatttgaa acattatgct tgaatgcatt ggagccaaat gtattaaatc aaatataaaa
     3901 ccaatatatt cataaagtta tatacttata gccatgctgt agcttgaaac agcccgccgc
     3961 ccgccctatt tacagcccat cgggacaaaa tgttccaaca tatttctcaa aataagcaaa
     4021 atcaaatagg attatccaca tggcaaaagt aatcggtatc gacttaggta caaccaactc
     4081 ttgtttggcc atttccgaaa acggtcaaac caaagttatc gaaaacgcag aaggcgcacg
     4141 caccacgccg tccgttatcg cttatttgga cggcggcgaa atcctcgtcg gcgcgcctgc
     4201 caaacgccaa gccgtaacca acgctaaaaa cactatttac gccgccaaac gtttgatcgg
     4261 ccacaaattt gaagacaaag aagtccaacg cgacatcgaa tctatgcctt ttgaaatcat
     4321 caaagccaac aacggcgacg catgggtgaa agcgcaaggc aaagagctgt ctcctcctca
     4381 aatttccgca gaagtcctgc gtaaaatgaa agaagccgcc gaagcttact tgggcgaaaa
     4441 agtaaccgaa gccgtgatta ccgttccggc ctacttcaac gacagccaac gtcaagctac
     4501 caaagacgca ggccgtatcg ccggtttgga cgtgaaacgc atcatcaacg agccgaccgc
     4561 agccgctttg gcattcggta tggacaaagg cgacaacaaa gaccgcaaag tagccgtata
     4621 tgacttgggc ggcggtactt tcgatatttc catcatcgaa atcgccaacc tcgacggcga
     4681 caaacaattt gaagtattgg ctaccaacgg cgatactttc ttgggcggtg aagacttcga
     4741 ccaacgcttg atcgactaca tcattgacga gtttaaaaaa gaacaaggca ttgatttgaa
     4801 acaagacgtc atggctctgc aacgtctgaa agaagctgcc gaaaaagcca aaatcgaatt
     4861 gtccagcggc cagcaaaccg aaatcaacct gccgtacatc accatggacg caaccggccc
     4921 gaaacacttg gcgatgaaaa ttacccgcgc caaattcgaa agcctggttg aagacctgat
     4981 tgcccgctct atcgagcctt gccgcaccgc attgaaagat gccggtctga gcactggcga
     5041 catcgacgac gtgattttgg tcggtggtca aagccgtatg ccgaaagtac aagaagccgt
     5101 taaagccttc ttcggcaaag agccgcgcaa agacgtgaac cctgacgaag ctgtcgccgt
     5161 aggcgcagcg attcaaggcg aagtgttaag cggcggccgc agcgacgtat tgctgctgga
     5221 cgtaacccct ctgtccttgg gtatcgaaac catgggcggc gtgatgacca agctgattca
     5281 aaagaacaca actattccga ctaaagcgtc gcaagtgttc tctaccgccg aagacaacca
     5341 aagcgcagta accatccacg tactgcaagg cgaacgcgaa cgcgcttctg ccaacaaatc
     5401 tttgggtcag ttcaacttgg gcgacatcgc acctgcacca cgcggtatgc cgcaaattga
     5461 agttactttc gacatcgacg ccaatggtat cttgcacgtt tctgccaaag acaaaggcac
     5521 cggtaaagca gccaacatta ccatccaagg ttcttcaggt ttgagcgaag aagaaatcga
     5581 acgcatggtg aaagatgccg aagccaatgc tgaggaagat aaaaagctga ctgaattggt
     5641 cgcttcccgc aaccaagccg aagccctgat tcactccgtg aaaaaatctt tggccgacta
     5701 cggcgacaaa ctcgacgctg ccgagaaaga aaaaatcgaa gccgcactga aagaagccga
     5761 agaagccgtg aaaggcgacg acaaaaccgc catcgatgcc aaagccgaag cactgggcac
     5821 agccagccaa aaactggggg aaatggttta cgctcaagca caagctgaag cccaagcagg
     5881 cgaaagcgaa caagccaatg cttctgcaaa gaaagacgat gatgtcgtag atgccgactt
     5941 tgaagaagta aaagacgaca aaaaataatt gatgccgtct gaaaaaagcg cgaaccgttt
     6001 gattcgcgct tttttcaatt gagataaaag accatagcat aacagaggtt ccaaattcat
     6061 ctaccgtgat attcccaagc ccaagttcta actgttatat cggttctctg gaaatttttt
     6121 atatagtgga ttaaatttaa accagtacag cgttgcctcg ccttagctca aagagaacga
     6181 ttctctaagg tgctgaagca
//
LOCUS       DAWFIE010000098         6141 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWFIE010000098 DAWFIE010000000
VERSION     DAWFIE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.098"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(832..3663)
                     /gene="lbpA"
                     /locus_tag="WGQ71_001850"
                     /gene_synonym="tbpA"
     CDS             complement(832..3663)
                     /gene="lbpA"
                     /locus_tag="WGQ71_001850"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ0707813.1"
                     /translation="MNKKHGFQLTLTALAIATAFPAYAAQAGGATPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTHDIQSNKTGQFFLVEGENTLK
                     PVAKLAGYGIYLNRQLNRWVKERIEKNQPLSAEEEAMVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEADKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKKLVRGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISESTG
                     FDDNNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADH
                     KTQLPDSSGRLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYHKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSAKRASSSWSTADVSAYLNLKKRLTLRAGIYNIGNYRYVTWESLRQTAEG
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(3660..5888)
                     /locus_tag="WGQ71_001851"
     CDS             complement(3660..5888)
                     /locus_tag="WGQ71_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0707814.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAGPSVETTPVNRPAVGAAMRLLRRNIATSDKDGNDFPNSRQAEEKLSFKEG
                     DVLFLYGSKKDQRQQLEDEIRQRNPNVQIRTSENENKKYGYNFVDAGYVYVKGEDEIE
                     RTSNYKYFTHRFGYDGFVYYSGEHPSQSLPSAGTVKYFGNWQYMTDAIRHRTGKAGDP
                     SEDLGYLVYYGQNVGATSYAATADDREGEGKHPAKYTVDFDKKILKGELIKNQYVQKR
                     NDPNEPKKPLTIYNITAKLDGNRFTGSAKVNPDLATNHANKEHLFFHTDADQRLEGGF
                     FGDNGEELAGRFISNDNSVFGVFAGKQKTETANAADTKPALPSGKHTKILDSLKISVD
                     EASDNNARQFAISSMPDLGHPDKLLVEGREIPLVNQEQTIDLADGRKMTVRACCDFLT
                     YVKLGRIKTDRPASKPKAEDKREDEESAGVGNGEEDEDEIADEDEVSEEDAEEIEEEA
                     DEVEEEEAEADEAEETEEESPTEESGSGSNAILPTPEAPKGRDIDLFLKGIRTAEADI
                     PKNGTAHYTGTWEARISTPIQWDNQADKTAKAEFNVDFGKKSISGTLTEKNGVEAAFH
                     IENGVIEGNGFHATARTRDNGINLSGTGSTNPKTFQANDLRVEGGFYGPQAAELGGTI
                     FNKDGKSLGITEGTENKVEVEAEVEVEAETGVVEQLEPDEVKHQFGVVFGAKKDNKEV
                     EK"
BASE COUNT         1341 a         1624 c         1420 g         1756 t
ORIGIN      
        1 tcagctattt agaataaatt ttgaaactct aatcgcgtca ttcccacgaa agtgggaatc
       61 cagttttttg agtttcagtc attcccgata aattgcctta gcattgaatg tctagattct
      121 cgcctgcgcg ggaatgacga atccatccat acggaaacct gcatcccgtc attcccacga
      181 acctgcatcc cgtcattccc acgaaagtgg gaatccagct ttttgagttt cagtcatttc
      241 cgagaaattg ccttagcatt gaatgtctag attcccgcct gcgcgggaat gacgggattt
      301 gaaattgcgg catttatcag gagcaacaga agccgctctg ccgtcattcc cacgaaagtg
      361 ggaatccagt ttttcgagtt tcagtcattc ccgataaatt gccttagcat tgaatgtcta
      421 gattcccgcc tgcgcgggaa tgacgaatcc atccgtacgg aaacctgcat cccgtcattc
      481 ccacgaaagt gggaatccag ctttttgagt ttcagtcatt tccgagaaat tgccttagca
      541 ttgaatgtct agattcccgc ctgcgcggga atgacggatt ttaggttggg ggcatttatt
      601 ggaaaaagca gaagccgctc tgccgtcatt cccgcgcaag cgggaatcca gtttcttgag
      661 tttcagtcat tcccgataaa ttgccttagc attgaatgtc tagattcccg cctgcgcggg
      721 aatgacggcg gaaatcttgt ttatatcgaa tcaaaaaaac ctgcacctta atcagttggc
      781 ggtttagtcc gacttttggg gtgcagatca agctttcaga cggtatttcc tttaaaactt
      841 catttcgagc gcgaggctga agttcctgcc cggtgcggca taccttccat agttgctgtc
      901 gccgccgtgc cggtttgccg tgccttccgc agtctggcgc aaggattccc aagtaacgta
      961 gcggtagttg ccgatattgt agatacccgc cctcaatgtc agccgttttt tcagattcag
     1021 ataggcggaa acgtctgccg tcgaccaaga agacgacgct ctttttgccg aatatcgttt
     1081 ttgatcgcct gccagataag caagttcatc agggtttttc cctttggaat aggtcagcat
     1141 aatgtttgcg ccccattttc cctcgggctg gtcgtatccg aaccccaaaa cataacgcga
     1201 cggctgtacc gcatccaaag catagctgcg gagagacagg cccggtcggt tggataccga
     1261 tttcggtttg atgcggttgt acgccaaagt cgtgtacaaa ccttcgggca gtttgtgata
     1321 cacgccgttc cagtcgattt ttcccaatat attaacgcct tggagcgaca tattttgggc
     1381 attgtaataa tcgcgtatat caatctctgt caatcgtcct gatgaatccg gcaattgggt
     1441 tttgtgatcg gcaacggcaa tcatatcggt atagcggttg cggaagctgc tgatttccaa
     1501 aaagccgaag tcgcccttcc actgcaaacc gatttcgcgg ttggctgcct tttccgattt
     1561 cagggcggga cgctgccagc ctttcggata atcgtgataa atgtctattc cgaaaagttc
     1621 ttggaaggag ggcgtcctga agccgctgga ggcgcggtaa gacaaagaaa aatgccggtt
     1681 cggtttgaac acgatgccgc tgttccacga acggtcaaca taccgtccgc tgcggacgag
     1741 ttcttccgac gtggtgaagt ttttccggtc gtacctgcca cccaagctga aatcaaaata
     1801 tttgccgatt gaaaaacggt cgttcaaaga aatgtggata ttgctgccgt tgatttttct
     1861 tggcacgcat ttgcgggaac gcagggtttc gatgtagccg cagaccgacc cttcgacgac
     1921 ttcgggctta cccaaacggt acttatcttg attgttatca tcgaatcccg tggattccga
     1981 aatccttgcc gcattgtggg aaagctgttc agggcgggaa atcgcgttgg aagcatcgta
     2041 accgaagccc aaagtcagat ggtgtttcgt ccatttgttt ttcagcgatt tctcaaacga
     2101 ggcattcaaa acattgtgct gttcgcggta gtggaaacgg tcgctgctgt cgtaggaata
     2161 cggtttgtcc gccgacgcgc ggcaggattt gtccacagca ggatacacgg cgcaattcag
     2221 cttcagcgtg ttgttgtcgg ttgccacgcc ctgtttgtca aacgacaaca ccgccttatc
     2281 cgcccagttg tcagaatacg cttcgttttc ataacggtac agcaaaccca tacggcggcg
     2341 gcggtgatgt tcatcaataa atttggtgcg ggaatatttc aaacctatgc ccctgaccaa
     2401 ctttttatcg cccttccact cttctatatt cggcacaaaa tacaagccgt cgcggaaatc
     2461 gtcgccgtcg tacaccccgc tcttgtctct aaacttgtcc gcctcgtccg taccgtaata
     2521 ctgtttttcc gtcatatcgc ggatgtcgta acgctgtttg gtatcctcaa acacgccgcc
     2581 gacataatgc ctgccgccga agcggtagcc cagcttggca agccaagagc cgctgcggta
     2641 atccatcgga tcgggcaata tcctgccgcc gcccgtgtaa gcttgggcgg acaggttttc
     2701 gtggcgcgcc tgcgcctccc gcaccatcgc ttcttcttca gcacttaaag gctgattttt
     2761 ttcaatacgt tcttttaccc agcggttaag ctggcggttc aaatatatcc cgtagcccgc
     2821 caattttgcc acgggcttga gtgtattctc gccctctact aagaaaaatt gccctgtttt
     2881 attgctttga atatcgtgtg tctggcggaa cgcgtccaaa cggtttatgc cgtatgccac
     2941 cccgtccgca atatcgccgt gcgggcgcgt ttcccgccct tggcgttcgg ttcggattaa
     3001 cagcccttcc caaccgtctt tgctgaaccc cgcgccgagc gacttcataa attggcggtt
     3061 tttactgccg taggcggttt ttgcctgtat cccccagctt ttgccgtctg aaatcaggtc
     3121 tgccgcctct ttggtgcgga aagcgaccgc gccgccgagt gcgccgctgc cgtgatcgga
     3181 cgaaccggca cctttgtcga tttccaccgt gctgatgttt tcatattcga tttcgttgat
     3241 tgcaccgctg ccgccgcgtc cgccgtatcc gctcaacgat ccctgcacgg taaacgcctg
     3301 tatttgggca acgccgtcaa ccgaaaccgc cacacggttt ttatccacgc cgcgtatcga
     3361 gtagccgccg ctcgcgccgt tgccctgttc gacaaccgcc acgcccggat cgtagcgcgt
     3421 caggtcgcgg ataccgagta cctgttcttt gttcaacgtt tccgacgttt tgacgatttt
     3481 gcccaaaccg gtcgcctctt tcgatcgccg tcccactttg gcggcacgga cggtaacctc
     3541 tttcagggat tgggtctgcg cggcatcagg tgtcgctccc cccgcttggg cagcataagc
     3601 cggaaaagcg gttgcaatgg ccaaggcggt cagggtaagc tgaaaaccgt gtttcttatt
     3661 catttttcca cctctttatt atctttcttc gcaccgaata ccacgccgaa ttggtgttta
     3721 acttcatcag gttctaactg ttcgacaaca ccagtttcag cttcaacttc aacttcagct
     3781 tcaacttcaa ctttattttc agtaccttca gttataccaa gagatttccc atccttattg
     3841 aaaatagtac cgcccaattc cgccgcctgc gggccgtaaa atcctccttc tacacgaaga
     3901 tcattagctt ggaaggtttt ggggttggtc gaaccagttc ccgaaagatt gatgccgtta
     3961 tcccgagtgc gcgctgtcgc gtggaaaccg ttgccctcaa tcacgccgtt ttcaatatgg
     4021 aaagcagctt ctacaccgtt tttctccgtc agcgttccgg aaatcgattt cttgccgaaa
     4081 tcaacattaa actctgcttt tgcggtttta tccgcctgat tgtcccattg aatgggtgtg
     4141 ctgatacgcg cttcccaagt gccggtataa tgcgccgttc cgttttttgg aatgtcggct
     4201 tccgccgtgc ggataccttt caggaaaagg tcgatgtccc tgcctttagg ggcttccgga
     4261 gtgggcagga tggcgtttga accgctgccg ctttcttctg tcggcgattc ttcttcggtt
     4321 tcttcggctt catcagcttc agcttcttcc tcttcaactt catcagcttc ttcttcgatt
     4381 tcttctgcat cttcttcgga aacttcgtct tcatcggcga tttcgtcttc gtcttcttcg
     4441 ccgttaccaa cgcctgcact ctcttcatcc tcccttttat cttccgcctt tggtttactt
     4501 gccgggcggt cggtttttat ccgtccgagt ttcacatagg tcagaaaatc gcaacaagca
     4561 cggacggtca ttttcctgcc gtcggcaaga tcgatggttt gttcttggtt taccaaagga
     4621 atttcacgcc cttcgacaag aagtttgtcg ggatgaccaa gatcgggcat agaggaaatg
     4681 gcaaactggc gggcattgtt atcacttgcc tcgtcaacgg aaattttcag agaatccaag
     4741 attttggtgt gttttccaga cggcagggca ggttttgtat ctgctgcgtt tgctgtctct
     4801 gttttttgtt tgcctgcgaa cacgccgaat acgctgttgt cgttgctgat aaaccgtccg
     4861 gcaagctctt ctccgttatc gccgaaaaaa ccgccctcaa gccgctgatc ggcatcggta
     4921 tggaaaaaca aatgctcctt attggcatgg tttgtcgcta aatcaggatt gaccttggca
     4981 ctgccggtaa agcggttgcc gtccaatttt gcagtaatgt tgtaaatggt cagcggtttt
     5041 ttaggttcat ttggatcatt tctcttttgc acatactgat ttttaatcag ctcgcccttt
     5101 aggatttttt tgtcgaaatc aaccgtatat ttggcaggat gttttccctc cccctcccgg
     5161 tcgtcggcag tcgcagcata agaagttgct ccgacatttt gaccgtaata aacgagataa
     5221 cccaaatctt cgctaggatc tcctgctttt cctgtccgat gacgtatggc atcggtcata
     5281 tattgccagt taccgaaata ttttaccgtt cccgcgctcg gtaaagattg cgaaggatgt
     5341 tctccggaat aatatacaaa accatcataa ccaaaccggt gagtaaaata cttgtaattt
     5401 gaagtcctct caatttcatc ttctcccttt acatatacat aacctgcatc tacaaagtta
     5461 taaccatatt ttttattttc attttctgat gtcctaattt gtacattagg attgcgttga
     5521 cgaatttcat cttcaagctg ctgacgttga tcttttttgg aaccgtataa aaacagaaca
     5581 tcaccttctt taaacgacag cttttcttct gcttgtctgc tatttggaaa atcattgcca
     5641 tccttatcag aagttgcgat attccgcctc aacagccgca ttgccgcacc gacggcgggc
     5701 cggttgaccg gcgtggtttc taccgaaggc ccggcagggg gcgaagtggg aacgtcctta
     5761 gacttgaaag tgacggggta cgcggtcggc gttgattcga caacaggctg tacgccgaaa
     5821 ttgccgccga tacaagatgc caaaagtaag ggcaacaaga caatgccgcc ataattcggt
     5881 ttacacatcc ctacttttcc tcctatttga ttaataataa ttatcattat attaatatgt
     5941 acagataata tcaacccgtt tttatagtga attaacaaaa atcaggacaa ggcgacgagc
     6001 cgcagacagt acagatacat tccgtcattc ccacgaacct acatcccgtc attcccacga
     6061 aagtgggaat ccagttcgtt cggtttcgct tgttttaagt ttcgggtaac ttccacttcg
     6121 tcattcccgc gaacctgcat t
//
LOCUS       DAWFIE010000099         6001 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWFIE010000099 DAWFIE010000000
VERSION     DAWFIE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.099"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(171..758)
                     /locus_tag="WGQ71_001852"
     CDS             complement(171..758)
                     /locus_tag="WGQ71_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ0707815.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTSKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            921..2327
                     /gene="dnaB"
                     /locus_tag="WGQ71_001853"
     CDS             921..2327
                     /gene="dnaB"
                     /locus_tag="WGQ71_001853"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ0707816.1"
                     /translation="MNDYTAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
     gene            2629..3297
                     /locus_tag="WGQ71_001854"
     CDS             2629..3297
                     /locus_tag="WGQ71_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ0707817.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERVANLLRFSKGEAVRLNLPVYICPVQVKKDGAPNNKCDSGKKGQGMLAFGDKNGNK
                     IYDGDAADVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTADRVVWTFNQNGTFGYST
                     NQDLTNTSKFVYSGGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRGDRRTM
                     CQYK"
     gene            3321..3929
                     /gene="pilV"
                     /locus_tag="WGQ71_001855"
     CDS             3321..3929
                     /gene="pilV"
                     /locus_tag="WGQ71_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ0707818.1"
                     /translation="MKNNDCFRLKDSQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYTGSYTPTSSDGDFKLDNL
                     KSKKDLAKVQLDRFGYELKNALPDAVAIHYAVCKDSSGKAPTLSGGTFSPNCDNKENG
                     DTLIKVLWVNDSAGDSDITRTNLETNGDNIVYTYQARVGGRE"
     gene            3926..4894
                     /locus_tag="WGQ71_001856"
     CDS             3926..4894
                     /locus_tag="WGQ71_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ0707819.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGLLSMIVLIAVGSSYFT
                     SRKLNDAANERLAEQQDLRNAATLIVRDARMAGSFGCFNMSEHTKDDIVDSSNQTQPA
                     SAKPGVKQENPLFSLKRSDMEQLIPVTESADIKYSDFTQRLNALVFQYGIDDLDASAE
                     TVVVSSCSKIAKPGKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYAVGRIADEE
                     GLFRFQLDDKGKWGNPQLLVKKVKRMNVRYIYVSGCPEDAGKEEQFKYTNKFDKSKNA
                     VTPAGVEVLLDSGLDAKIAASSDNSIYAYRINATIRGGNVCANRTL"
     gene            4873..5472
                     /locus_tag="WGQ71_001857"
     CDS             4873..5472
                     /locus_tag="WGQ71_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ0707820.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGEFQVLDLEYDTDSKVTFSENCEKGLCTAVNVRT
                     NTNHGNEEAFDNIVVQGNPTVEAVKRSCPANSTDLCIDNKGMEYKKGTQSVSKPPRYI
                     IEYLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            5477..5950
                     /locus_tag="WGQ71_001858"
     CDS             5477..5950
                     /locus_tag="WGQ71_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ0707821.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIERGYQSQLYTE
                     MVGINNVLKQIILKNPQDDNDTLKIRLEALASGYKMNPKIAKKYSVSVAFANTEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDKASAEAYSETLSADAGCEAFSNRKK"
BASE COUNT         1622 a         1344 c         1546 g         1489 t
ORIGIN      
        1 tgacagtaac cgtagcaact gaaccgtcat tcccacggaa gtgggaatct agaaatgaaa
       61 agcaacaggc atttatcgga aataactgaa accgaacaga cctagattcc cgcctgcgcg
      121 ggaatgacgg ctgcagatgc ccgacggtct ttatagcgga ttaacaaaaa tcaggacaag
      181 gcggcgaagc gtttggcgac ttcgtcccag ttgacgattt cccaaaaacc tttcaggtag
      241 ttgggacggc tgttgcggta gtcgatgtag taggcgtgtt cccacacgtc gcaggtcagc
      301 agcggcgtgt tttcagtggt cagcggcgta gcggcgttgg aagtggaaac caaatccaat
      361 ccgccggcag gggtttttac cagccacgcc caaccggagc cgaaagtacc ggccgcgcag
      421 gcattgaacg cttcttggaa tttctcgaag ctgccccatt tcgcgtcgat ggcggcggcc
      481 agttcgccgg caggtttgcc ttgacctttg gacgtgaaac ccagccagta gaaggtgtgg
      541 ttccaagttt gtgccgcgtt gttgaacacg ccgcccgaag attttttcac aatctcttcc
      601 aaaggcaggt tttcaaattc ggtacctttg atttgattgt tcaggttggt gatgtaggtt
      661 tgatggtgtt tgccgtagtg gaattccaaa gtttctttgc tcagatgcgg ggacaatgcg
      721 tccagttcat aaggcagttg cggcagctta tgttccattt tgtgctccta atattgtttt
      781 aaatgttgta ttttggcagt gttgctgcaa atagcttggc agcgcacata ttttacctgt
      841 tttgagggat ggaaaccaat taaacctgct ttacgctata atagaagatt gcaatttcgg
      901 cacgacagat aggatgtacc atgaacgatt acacagctat gccgtctgaa gaccgtgagg
      961 tcggcgcatt gtcgctgcct ccacattcga tggaggcgga acaatccgtt ttgggcggtt
     1021 tgatgctgga aaatccggct tgggacagga ttgccgatgt ggtttcggga gaggatttct
     1081 accgccacga acaccgcctg attttccgat ccattgctaa attaatcaat gagagccgtc
     1141 ccgccgatgt gattacggtt caggaagatt tgcagcggaa cgaagaattg gaagcggcag
     1201 gcggatttga atatctgatt acgctggcgc aaaatacccc gtctgccgcc aacatccgcc
     1261 gctatgccga aatcgtgcgc gagcgttcca tcatgcgcca actcgccgaa gtggggacgg
     1321 aaatcgcccg cagcgcatac aatccgcaag gcagggacgc gggtcagctt ttggacgagg
     1381 cggaaaacaa agtattccaa atcgccgaaa gcaccgccaa atccaagcag ggctttttgg
     1441 agatgcccga tttgctgaaa gaagtcgtac agcgcatcga tatgctctac tcgcgcgaca
     1501 atcccgatga agttaccggc gtgccgacgg ggttcatcga cctcgacaaa aaaacctcgg
     1561 gccttcagcc cggcgacctg attattgttg ccggtcgtcc gtctatgggt aagaccgcct
     1621 tttctatcaa tatcgccgaa cacgttgccg tagaaggcaa gctgccggtt gccgtcttct
     1681 caatggagat gggcggggcg caactggtca tgcgtatgct cggctcggtc ggacggctgg
     1741 atcaaagcgt cctgaaaacc ggcaggctcg aagacgaaca ctggggtcgt ctgaacgaag
     1801 cagtcgtcaa actctccgac gcgcccgtgt acatcgacga gaccccgggt ttgactgcgc
     1861 tcgaactgcg cgcccgcgcc cgtcgtctcg cccgtcaatt taacaataag cttggattaa
     1921 tcgtcatcga ctacctgcaa ctgatggcgg gatccggccg ttcggacaac cgagcttcgg
     1981 agctgggcga aatttcccgc tccctcaaag ccctggcgaa agaattgcaa gtccccatca
     2041 tcgccctgtc gcaattgagc cgtaccgtcg aatcgcgtac cgacaaacgc ccgatgatgt
     2101 ccgaccttcg cgagtccggc gcaatcgagc aggatgccga cctgattatg ttcatgtatc
     2161 gcgacgaata ctacaaccaa gactcaccca tgaaaggcct tgccgaatgt atcatcggca
     2221 aacaccgcaa cggtcccgtc ggtaaaatct tcctcacatg gacgggacaa ttcaccaaat
     2281 tcgacaatgc tgcctatatt cccgaggagg caaagataga agattaaatg gctatataaa
     2341 aatttattag gcgaaatcag gcaaaatcgt ttaaaatcat gctgagagat tgccctaaaa
     2401 aataaaacgc ggtcttgagg catttttgca ttcagcccgc atataattga aaatatagtg
     2461 gattaacaaa aatcaggaca aggcaacgaa gccgcagaca gtacaaatag tacggaaccg
     2521 attcacttgg tgcttgagca ccttagagaa tcgttctctt tgagctaagg cgaggtaacg
     2581 ccgtactggt ttgaatttaa tccacgatac attaccagtt aacgtcctat gtgtacacga
     2641 aaacaacaag gtttcacgct aacagagctg ctcatcgtga tggtcatcgc agccattatg
     2701 gcgatgatag ccctccccaa tatgagccaa tggattgcat cccgccgcat tgccagtcac
     2761 gcggagcggg ttgccaacct tttgcgtttc tccaagggcg aagccgtccg gctcaatctc
     2821 cctgtctata tctgtcctgt tcaagttaaa aaagacggtg cgcccaacaa taaatgtgac
     2881 tccggcaaga aggggcaggg aatgttggct tttggcgata agaatggcaa taagatatat
     2941 gacggcgatg cggcggatgt tcttctccgc agtgtggtat tgaatgatga tatcaatgat
     3001 aagcggatta attatgcctt caaccatatc gctttcggtc agactcagcc gaccgctgac
     3061 cgtgtagttt ggacattcaa tcaaaacggg acgttcggtt attctaccaa tcaggatctc
     3121 acgaatactt ccaaatttgt ttactccggc ggttatatcc aaatcgtgct gacagatgcg
     3181 agagcagttt cagatgccga taagaaattc cgttcggcgg tggttttgat tgacagcagc
     3241 ggcagggtcg aagtttgccc taggggtgat cggcgtacta tgtgccagta taaataacag
     3301 tttcagtttt aaaaatgaat atgaagaata atgattgctt ccgcctgaaa gattcccagt
     3361 ccggtatggc gctgatagaa gtcttggttg ctatgctcgt tctgaccatc ggtattttgg
     3421 cgttactgtc tgtgcagttg cggacagtcg cttccgtcag ggaggcggag acacaaacca
     3481 tcgtcagcca aatcacgcaa aacctgatgg aaggaatgtt gatgaatccg accattgatt
     3541 cggacagcaa caagaaaaac tataatcttt acactgggtc gtacaccccc acttcctctg
     3601 acggcgattt caagcttgat aatttgaaaa gcaagaagga tttggcaaag gtccagttgg
     3661 acaggttcgg ttatgaattg aaaaatgcct tgccggatgc ggtagctatt cattacgctg
     3721 tctgcaagga ttcgtcgggt aaagcgccga cattgtccgg cggtactttt tctccaaatt
     3781 gcgataataa ggaaaacgga gatactttaa ttaaagtatt gtgggtaaat gattcggcag
     3841 gggattcgga tatcacccgt acgaatcttg agacgaacgg cgacaatatc gtatatacct
     3901 atcaggcaag ggtcggaggt cgggaatgag acgtaaaatg ctaaacgtac caaaaggcag
     3961 ttatgatggt atgaaaggtt ttaccattat tgaatttttg gttgcgggcc tgctcagtat
     4021 gattgtcctg atagcggtcg gatcgagtta cttcacatcc cggaaattaa atgatgcggc
     4081 aaacgagcgt cttgccgagc aacaggattt gcggaatgcg gcaacattga ttgtccgcga
     4141 tgcaagaatg gcggggagct tcggttgttt caatatgtcc gagcatacta aagacgatat
     4201 tgttgattca agtaatcaaa ctcaacctgc ctctgcaaaa cccggtgtca aacaagaaaa
     4261 tccccttttt tccttaaaaa ggagcgacat ggaacaactg attcccgtta ctgaatccgc
     4321 agatattaaa tattcggatt ttacccagcg ccttaacgca ttggttttcc aatacggtat
     4381 cgatgatctt gatgcgagtg ctgagactgt tgtagtcagc agctgttcca aaatagcaaa
     4441 accgggtaag aaaatatcta ccttgcaaga agcaaagagt gcattacaga ttactaatga
     4501 tgataaacaa aatggaaata tcacccgtca aaggcatgtg gtcaatgcct atgcggtcgg
     4561 caggattgcc gatgaggaag gtttgttccg cttccaattg gatgataagg gcaagtgggg
     4621 taatcctcag ttgctcgtga aaaaggttaa acgtatgaat gtgcggtata tttatgtttc
     4681 cggttgtcct gaagatgccg gcaaagagga gcaattcaaa tatacgaata aattcgacaa
     4741 atccaaaaat gctgttacgc ctgccggggt ggaggtttta ttggatagcg gccttgatgc
     4801 caagattgcc gcttcttcag acaatagtat ttatgcttac cgtatcaatg cgacaatacg
     4861 ggggggaaat gtatgcgcaa acagaacact ttgacgggaa tcccgacttc tgacggacag
     4921 agggggtttg cactgtttat cgtgctgatg gtaatgatcg tcgtggcttt tttggttgta
     4981 actgccgcgc agtcttacaa taccgagcag cggatcagtg ccaacgaatc agacaggaaa
     5041 ttggctttgt ctttggccga ggcggctttg cgggaaggcg aatttcaggt tttggatttg
     5101 gaatatgata cggacagtaa ggttacattt agcgaaaact gtgaaaaagg tctgtgtacc
     5161 gcagtgaatg tgcggacaaa tacaaatcat ggtaatgaag aggcttttga caatatcgtg
     5221 gtgcaaggca atcccaccgt tgaggcggtg aagcgttctt gccctgcaaa ttctaccgac
     5281 ctgtgcattg acaataaagg gatggaatat aagaaaggca cgcaaagtgt cagcaaacca
     5341 ccgcgctata ttatcgaata tttgggcgtg aagaacggag aaaatgttta tcgggttact
     5401 gccaaggctt ggggtaagaa tgccaatacc gtggtcgtcc ttcaatctta tgtaagcaat
     5461 aatgatgagt aataaaatgg aacaaaaagg gtttacattg attgagatga tgatagtcgt
     5521 cgcgatactc ggcatcatca gcgtcattgc cataccttct tatcagagtt atattgaaag
     5581 aggctatcag tcccagcttt atacggagat ggtcggtatc aacaatgttc tcaaacagat
     5641 tattttgaaa aatccccagg acgataatga taccctcaag atacgactgg aagcgcttgc
     5701 ctcaggctat aagatgaatc cgaaaattgc caaaaaatat agtgtttcgg tggcatttgc
     5761 caatacggaa aaaccaaggg catacagctt ggtcggtgtt ccaaagacgg ggacgggtta
     5821 tactttgtcg gtatggatga acagcgtggg cgacggatac aaatgccgtg ataaggcttc
     5881 tgccgaagcc tattcggaga ccttgtccgc agatgccggc tgtgaagcct tctctaatcg
     5941 taaaaaataa ggttgttttg ccgatgccgt ctgaaaatca atgttcagac ggcattttta
     6001 t
//
LOCUS       DAWFIE010000100         5824 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWFIE010000100 DAWFIE010000000
VERSION     DAWFIE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.100"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(43..999)
                     /locus_tag="WGQ71_001859"
                     /pseudo
     CDS             complement(43..999)
                     /locus_tag="WGQ71_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1235..2200
                     /locus_tag="WGQ71_001860"
     CDS             1235..2200
                     /locus_tag="WGQ71_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0707822.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKAAFEKAEPVAAGKE"
     gene            3183..3506
                     /locus_tag="WGQ71_001861"
     CDS             3183..3506
                     /locus_tag="WGQ71_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0707823.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSVVLIELSD
                     PSEAV"
     gene            3620..5797
                     /locus_tag="WGQ71_001862"
     CDS             3620..5797
                     /locus_tag="WGQ71_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0707824.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRLTRKPLFEVRAEAGNRKHFGLDADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRRRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPSSFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQGETHTDPATLRIPNPAAKARAAANSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT         1340 a         1802 c         1634 g         1048 t
ORIGIN      
        1 taatggttgt ttcaaatcaa aatatctatg ccgccggccc catcagcact tcttcacatc
       61 cgaaggcaaa aatccgtaat gccgtctgaa cgcttcgttg aaccgtcccg cgtggcggta
      121 gccgcaaaag tgcatggcgg cttggacggt gctgccggat tcgatgaggg cgagcgcgtg
      181 ttccagccgc aggcggcgca ggcatccggc gacggtttcg ccggtttgcg ctttgaaata
      241 gcgtttcagg tagcattcgt tcagtccgac gcggcgggcg atttcggcga tggtcagcgg
      301 acgggcgaat tcgtgttgca ggatgtcggc ggcttcgtct atgcgccgac ggcggtaacc
      361 gttgtcgtgg cggcggaagg tgaagcgcaa taatcgggcg gagagttcca gcgcggcggc
      421 ttcgtcggca agcaggccga agccgtccga ttcgaacggg cgttgcagca gtgggcaggc
      481 cgccgccgtc agtgccgctg cgttttgcgc cagccgttac agggcgaatc ggcctatcgt
      541 ttgcggcgaa aacaggcgtt cgtccagcaa gccttcgtcg tgccagcggc gcagtttttc
      601 cagcgaaaaa tccaaatgca gcgcgcacat gccgctgttg tcgggcagca gggtttcgga
      661 tacgtccgcc aactcgccgc gtaccagcca gatttcgccg gcagacgggc ggtattccct
      721 gccgcccatt tgtaaccggt tctgccccga caccatgacg aacaaggcgc agttgtggct
      781 gaaattgtgg atttcggtgg gaaacgcgcc cgttccgccg ccgcgcatcc gcgacagggt
      841 gatgcccgag tcgaagcggt tgatgcacat ttccagatgc aaaccgggct gttttgcctg
      901 cgcaatgagc gcgctgtcgg aacagccgtc caacgcccag ccggatttat cggagcggac
      961 ataggtttgg tactggcggt agatggcggc ggtgttcatg attggatagg aacgagttgt
     1021 ctaacaaatg aattaaatag gaattattac caataatcaa gcgcagggat tggttgaaac
     1081 ggaaaaggtc gtctgaaagg gtgtttcaga cgaccttttc cgtatcggga atttgttttg
     1141 ccgtatcggg aattttgcgt tttgcggcgt ggtttctgca ggttgtttgc ttaataataa
     1201 acattcttat tcgtatgcaa aggaaccgca caccgtgaaa ccgcgttttt attgggcagc
     1261 ctgcgccgtc ctgctgaccg cctgttcgcc cgaacctgcc gccgaaaaaa ctgtatccgc
     1321 cgcatccgca tctgccgcca cactgaccgt gccgaccgcg cggggcgatg ccgttgtgcc
     1381 gaagaatccc gaacgcgtcg ccgtgtacga ctgggcggcg ttggatacgc tgaccgaatt
     1441 gggtgtgaat gtgggcgcaa ccaccgcgcc ggtgcgcgtg gattatttgc agcctgcatt
     1501 tgacaaggcg gcaacggtgg ggacgctgtt cgagcccgat tacgaagccc tgcaccgcta
     1561 caatcctcag cttgtcatta ccggcgggcc gggcgcggaa gcgtatgaac agttggcgaa
     1621 aaacgcgacc accatagatc tgacggtgga caacggcaat atccgcacca gcggcgaaaa
     1681 gcagatggag accttggcgc ggattttcgg caaggaagcg cgcgcggcgg aattgaaggc
     1741 gcagattgac gcgctgttcg cccaaacgcg cgaagccgcc aaaggcaaag gacgcgggct
     1801 ggtgctgtcg gttacgggca acaaggtgtc cgccttcggc acgcagtcgc ggttggcaag
     1861 ttggatacac ggcgacatcg gcctaccgcc tgtagacgaa tctttacgca acgaggggca
     1921 cgggcagcct gtttccttcg aatacatcaa agagaaaaac cccgattgga ttttcatcat
     1981 cgaccgtacc gccgccatcg ggcaggaagg gccggcggct gtcgaagtat tggataacgc
     2041 gctggtacgc ggcacgaacg cttggaagcg caagcaaatc atcgtcatgc ctgccgcgaa
     2101 ctacattgtc gcgggcggct cgcggcagtt gattcaggcg gcggagcagt tgaaggcggc
     2161 gtttgaaaag gcagaacccg ttgcggcggg gaaagagtag ggagtcgtct gaaaacggag
     2221 cttccgaagg aagcgggggg tttctgcgaa gctaaagtgc ggtttcaacg aattgaaaag
     2281 cagcctgtat tttgaaaata ccgctcaagc aaacctacgg tttgccgccc tctccctagc
     2341 cctctcccac agggagaggg gattgggttg caggctgcct ttaaggttta ggcaaatttt
     2401 taacttcgtt gaagctccgt tttcagacga ccttttggag taccgcaggc acacgcatcg
     2461 aacggctgaa tcaaagttca gaccgatggc agcccgcacc cgagtttatg cggcaaacag
     2521 cgaggctacg gcaacccgtc ccctctccct gtgggagagg gttagggaga gggcagtaag
     2581 ccgtaggctt acatcaaagt cgataacgct ttcacaaagc ctgtgtgcac tgaaaactga
     2641 aaggtcatct gaaaacggag ctttcgaagg aagcagagtt tctgcgaagc taaaaccgaa
     2701 gctgcaaaaa atcgaaaagc agcctgcacg ttgaaaatgc cgcccaagca aacctgcggt
     2761 ttgccgccct ctccctagcc ctctcccaca gggagagggg attggggtgc aggctgtctt
     2821 taaggttcag gcaaattttt aacttcgttg aagctgcgat ttcagaagct ccgttttagc
     2881 ttcgcagaaa ctctgcttcc ttcgaaagct ccgttttcag acgacctttt ggagtaccgc
     2941 aggcacacgc atcgaacggc tgaatcaaag ttcagaccga tggcagcccg cacccgagtt
     3001 tatgcggcaa acagcgaggc tacggcaacc cgtcccctct ccctgtggga gagggttagg
     3061 gagagggcgg taagccgcag gcttgcatca aagtcgataa cgctttcgtt acaactccgc
     3121 ccactgaacg aagccaaaac gacaaaccgc atcgtaaacc acccaaccca taggagaacc
     3181 ccatgcaaaa cgaaaccatc aacctgaaac agcaccttgc cgccatcaaa gaatactggc
     3241 agcccgaaat catcaaccgc cacgggttcc aattccgttt ggtcaaactt ttgggcgatt
     3301 acggctggca tacgcacgga tacagcgaca aagtgctgtt tgccgtggag ggcgacatgg
     3361 cggtggactt cgccgacggc ggcagcatga cgatacgcga gggcgagatg gcggtcgtgc
     3421 cgaagtcggt gtcgcaccgc ccgcgttcgg aaaacggctg ctcggtggtg ctgattgagt
     3481 tgtccgaccc gtccgaggcc gtctgaaaac gaagtttccg aaggaagctg agtttctgcg
     3541 aagctaaaag cagcctgcac cttcaatcaa tatgccgaaa atacaacccc accgcacacc
     3601 aacacacaaa ggaaatccca tgacacgctt caaatattcc ctgctgtttg ccgccctgtt
     3661 gcccgtgtac gcgcaggccg atgtttctgt ttcagacgac cccaaaccgc aggaaagcac
     3721 tgaattgccg accatcaccg ttaccgccga ccgcaccgcg agttccaacg acggctacac
     3781 tgtttccggc acgcacaccc cgctcgggct gcccatgacc ctgcgcgaaa tcccgcagag
     3841 cgtcagcgtc atcacatcgc aacaaatgcg cgaccaaaac atcaaaacgc tcgaccgcgc
     3901 cctgttgcag gcgaccggca ccagccgcca gatttacggc tccgaccgcg cgggctacaa
     3961 ctacctgttc gcgcgcggca gccgcatcgc caactaccaa atcaacggca tccccgttgc
     4021 cgacgcgctg gccgatacgg gcaatgccaa caccgccgcc tatgagcgcg tagaagtcgt
     4081 gcgcggcgtg gcggggctgc tggacggcac gggcgagcct tccgccaccg tcaatctggt
     4141 gcgcaaacgc ctgacccgca agccattgtt tgaagtccgc gccgaagcgg gcaaccgcaa
     4201 acatttcggg ctggacgcgg acgtatcggg cagcctgaac accgaaggca cgctgcgcgg
     4261 ccgcctggtt tccaccttcg gacgcggcga ctcgtggcgg cggcgcgaac gcagccgcga
     4321 tgccgaactc tacggcattt tggaatacga catcgcaccg caaacccgcg tccacgcagg
     4381 catggactac cagcaggcga aagaaaccgc cgacgcgccg ctcagctacg ccgtgtacga
     4441 cagccaaggt tatgccaccg ccttcggccc gaaagacaac cccgccacaa attgggcgaa
     4501 cagccgccac cgtgcgctca acctgttcgc cggcatcgaa caccgcttca accaagactg
     4561 gaaactcaaa gccgaatacg actacacccg cagccgcttc cgccagccct acggcgtagc
     4621 aggcgtgctt tccatcgacc acaacaccgc cgccaccgac ctgattcccg gttattggca
     4681 cgccgacccg cgcacccaca gcgccagcgt gtcattgatc ggcaaatacc gcctgttcgg
     4741 ccgcgaacac gatttaatcg cgggtatcaa cggttacaaa tacgccagca acaaatacgg
     4801 cgaacgcagc atcatcccca acgccattcc caacgcctac gaattttccc gcacgggcgc
     4861 ctatccgcag ccatcatcgt ttgcccaaac catcccgcaa tacggcacca ggcggcaaat
     4921 cggcggctat ctcgccaccc gtttccgcgc cgccgacaac ctttcgttga tactcggcgg
     4981 cagatacagc cgttaccgca ccggcagcta cgacagccgc acacaaggca tgacctatgt
     5041 gtccgccaac cgtttcaccc cctacacagg catcgtgttc gacctgaccg gcaacctgtc
     5101 gctttacggc tcgtacagca gcctgttcgt cccgcaatcg caaaaagacg aacacggcag
     5161 ctacctgaaa cccgtaaccg gcaacaatct ggaagccggc atcaaaggcg aatggcttga
     5221 aggccgtctg aacgcatccg ccgccgtgta ccgcgcccgt aaaaacaacc tcgccaccgc
     5281 agcaggacgc gacccgagcg gcaacaccta ctaccgcgcc gccaaccaag ccaaaaccca
     5341 cggctgggaa atcgaagtcg gcggccgcat cacgcccgaa tggcagatac aggcaggtta
     5401 cagccaaagc aaaacccgcg accaagacgg tagccgcctg aaccccgaca gcgtacccga
     5461 acgcagcttc aaactcttca ctgcctacca ctttgccccc gaagccccca gcggctggac
     5521 catcggcgca ggcgtgcgct ggcagggcga aactcacacc gaccctgcca cgctccgcat
     5581 ccccaacccc gctgccaaag cccgcgccgc cgccaacagc cgccaaaaag cctacgccgt
     5641 cgccgacatc atggcgcgtt accgcttcaa tccgcgcgcc gaactgtcgc tgaacgtgga
     5701 caacctgttc aacaaacact accgcaccca gcctgaccgc cacagttacg gcgcactgcg
     5761 gacagtgaac gcggcgttta cctatcggtt taaataaggt cgtctgaaaa cggagtttct
     5821 gcga
//
LOCUS       DAWFIE010000101         5726 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWFIE010000101 DAWFIE010000000
VERSION     DAWFIE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.101"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..264)
                     /locus_tag="WGQ71_001863"
     CDS             complement(<1..264)
                     /locus_tag="WGQ71_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ0707825.1"
                     /translation="MLCPEKMSGMAGQYPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEA"
     gene            complement(300..413)
                     /gene="rrf"
                     /locus_tag="WGQ71_001864"
     rRNA            complement(300..413)
                     /gene="rrf"
                     /locus_tag="WGQ71_001864"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(510..3401)
                     /locus_tag="WGQ71_001865"
     rRNA            complement(510..3401)
                     /locus_tag="WGQ71_001865"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3808..3883)
                     /locus_tag="WGQ71_001866"
     tRNA            complement(3808..3883)
                     /locus_tag="WGQ71_001866"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3848..3850),aa:Ala,seq:tgc)
     gene            complement(3889..3965)
                     /locus_tag="WGQ71_001867"
     tRNA            complement(3889..3965)
                     /locus_tag="WGQ71_001867"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3929..3931),aa:Ile,seq:gat)
     gene            complement(4067..5607)
                     /locus_tag="WGQ71_001868"
     rRNA            complement(4067..5607)
                     /locus_tag="WGQ71_001868"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1245 a         1672 c         1228 g         1581 t
ORIGIN      
        1 cgcctccaac tcctgctgcg aagccgcatt gatgtttacc gccgcaaggg agaaggcgca
       61 ggagaacagc atacagaaca gcacgaacat tttcttcatg gtttttcctt taagggttgc
      121 aaacaataaa ccgcatcttg cgacgataaa acgagtcatt ctaaaatgaa tatcccaaag
      181 tttcaagccg ttcctccgca aacccgaccg gacgccgtac ggatactgtc ccgccatccc
      241 cgacattttt tccgggcaaa gcaaaaaccc ccgaataatc gggggttttc tgaatgggtg
      301 tttggcagtg acctactttc gcatggaaga accacactat catcggcgct gagtcgtttc
      361 acggtcctgt tcgggatggg aaggcgtggg accaactcgc tatggccgcc aaacttaaac
      421 tgttacaaat cggtaaagcc ttaatcaata tattcggtaa tgactgaatc agtcagtaag
      481 cttttatctc ttgaagttct tcaaatgata gagtcaagcc tcacgagcaa ttagtatggg
      541 ttagcttcac gcgttaccgc gcttccacac cccacctatc aacgtcctgg tctcgaacga
      601 ctctttagtg cggttaaacc gcaagggaag tctcatcttc aggcgagttt cgcgcttaga
      661 tgctttcagc gcttatctct tccgaactta gctacccggc tatgcaactg gcgttacaac
      721 cggtacacca gaggttcgtc cactccggtc ctctcgtact aggagcagcc cccgtcaaac
      781 ttccaacgcc cactgcagat agggaccaaa ctgtctcacg acgttttaaa cccagctcac
      841 gtaccacttt aaatggcgaa cagccatacc cttgggaccg actacagccc caggatgtga
      901 tgagccgaca tcgaggtgcc aaactccgcc gtcgatatga actcttgggc ggaatcagcc
      961 tgttatcccc ggagtacctt ttatccgttg agcgatggcc cttccataca gaaccaccgg
     1021 atcactatgt cctgctttcg cacctgctcg acttgtcggt ctcgcagtta agctaccttt
     1081 tgccattgca ctatcagtcc gatttccgac cggacctagg taaccttcga actcctccgt
     1141 tacgctttgg gaggagaccg ccccagtcaa actgcctacc atgcacggtc cccgacccgg
     1201 atgacgggtc tgggttagaa cctcaaagac accagggtgg tatttcaagg acggctccac
     1261 agagactggc gtctctgctt ctaagcctcc cacctatcct acacaagtga cttcaaagtc
     1321 caatgcaaag ctacagtaaa ggttcacggg gtctttccgt ctagcagcgg gtagattgca
     1381 tcttcacaac cacttcaact tcgctgagtc tcaggaggag acagtgtggc catcgttacg
     1441 ccattcgtgc gggtcggaac ttacccgaca aggaatttcg ctaccttagg accgttatag
     1501 ttacggccgc cgtttactgg ggcttcgatc cgatgctctc acatcttcaa ttaaccttcc
     1561 agcaccgggc aggcgtcaca ccctatacgt ccactttcgt gttagcagag tgctgtgttt
     1621 ttaataaaca gtcgcagcca cctattctct gcgaccctcc ggggcttacg gagcaagtcc
     1681 ttaaccttag agggcatacc ttctcccgaa gttacggtat caatttgccg agttccttct
     1741 cctgagttct ctcaagcgcc ttagaattct catcctgccc acctgtgtcg gtttgcggta
     1801 cggttcgatt caaactgaag cttagtggct tttcctggaa gcgtggtatc ggttgcttcg
     1861 tgtccgtaga cactcgtcgt cacttctcgg tgttaagaag acccggattt gcctaagtct
     1921 tccacctacc ggcttaaaca agctattcca acagcttgcc aacctaacct tctccgtccc
     1981 cacatcgcat ttgaatcaag tacaggaata ttaacctgtt tcccatcgac tacgcatttc
     2041 tgcctcgcct taggggccga ctcaccctac gccgatgaac gttgcgtagg aaaccttggg
     2101 ctttcggcga gcgggctttt cacccgcttt atcgctactc atgtcaacat tcgcacttct
     2161 gatacctcca gcacacttta caatgcacct tcatcggcct acagaacgct cccctaccat
     2221 gccggtaaac cggcatccgc agcttcggtt atagatttga gccccgttac atcttccgcg
     2281 caggacgact cgaccagtga gctattacgc tttctttaaa tgatggctgc ttctaagcca
     2341 acatcctggc tgtctgggcc ttcccacttc gtttaccact taatctatca tttgggacct
     2401 tagctggcgg tctgggttgt ttccctcttg acaacggacg ttagcacccg ctgtctgtct
     2461 cccgaggaac cacttgatgg tattcttagt ttgccatggg ttggtaagtt gcaataaccc
     2521 cctagccata acagtgcttt acccccatca gtgtcttgct cgaggcacta cctaaatagt
     2581 tttcggggag aaccagctat ctccgagttt gtttagcctt tcacccctat ccacagctca
     2641 tccccgcatt ttgcaacatg cgtgggttcg gtcctccagt acctgttacg gcaccttcaa
     2701 cctggccatg gatagatcac tcggtttcgg gtctacaccc agcaactcat cgccctatta
     2761 agactcggtt tccctacgcc tcccctattc ggttaagctc gctactgaat gtaagtcgtt
     2821 gacccattat acaaaaggta cgcagtcaca cctctagggt gctcccactg tttgtatgca
     2881 tcaggtttca ggttctattt cactcccctc ccggggttct tttcgccttt ccctcacggt
     2941 actggttcac tatcggtcga tgatgagtat ttagccttgg aggatggtcc ccccatattc
     3001 agacaggatt tcacgtgtcc cgccctactt ttcgtacgct tagtaccgct gttgagattt
     3061 cgaatacggg actgtcaccc actatggtca agcttcccag cttgttcttc tatctcgaca
     3121 gttattacgt acaggctcct ccgcgttcgc tcgccactac ttgcggaatc tcggttgatt
     3181 tcttttcctc cgggtactta gatggttcag ttctccgggt tcgcttctct aagtctatgt
     3241 attcaactta ggatactgca cagaatgcag tgggtttccc cattcggaca tcgcgggatc
     3301 attgctttat tgccagctcc cccgcgcttt tcgcaggctt acacgtcctt cgtcgcctat
     3361 catcgccaag gcatccgcct gatgcactta ttcacttgac tctatcattt caagaacttc
     3421 tttgactttg cctaacattc cgttgactag aacatcagac ttgaatttcc tactttgata
     3481 aagcttactg ctttgttgtg tcttaatcct gccttttgtg tttcaggatt aagtcgatac
     3541 aatcatcacc caaatactgt gtttgttttc ttttctcttg tgagcgattt tttatccttt
     3601 gcaaagaata aaaaatcaaa acaaacgctt tgtctttgtt tgttgatttc ggctttccaa
     3661 tttgttaaag atcgatgcgt tcgatattgc tatctactgt gcaaatcaaa acgagctgat
     3721 tattatatca gcattttgtt cttggtcaag tgtgacgtcg ccctgaatgg attctgttcc
     3781 attcttccgt tttgatttgt acagtattgg tggaggcaaa cgggatcgaa ccgatgaccc
     3841 cctgcttgca aagcaggtgc tctaccaact gagctatgcc cccgttcttg gtgggtctgg
     3901 gaggacttga acctccgacc ccacgcttat caagcgtgtg ctctaaccag ctgagctaca
     3961 aacccggatt ctcttcttaa gcgaatcttg ccttcactca agcttcttcc gcatcttttt
     4021 cagtttaccg ataagtgtga atgcctaaag cctcttcttt ctctagaaag gaggtgatcc
     4081 agccgcaggt tcccctacgg ctaccttgtt acgacttcac cccagtcatg aagcataccg
     4141 tggtaagcgg actccttgcg gttatcctac ctacttctgg tatcccccac tcccatggtg
     4201 tgacgggcgg tgtgtacaag acccgggaac gtattcaccg cagtatgctg acctgcgatt
     4261 actagcgatt ccgacttcat gcactcgagt tgcagagtgc aatccggact acgatcggtt
     4321 ttgtgagatt ggctccgcct cgcggcttgg ctaccctctg taccgaccat tgtatgacgt
     4381 gtgaagccct ggtcataagg gccatgagga cttgacgtca tccccacctt cctccggctt
     4441 gtcaccggca gtctcattag agtgcccaac tgaatgatgg caactaatga caagggttgc
     4501 gctcgttgcg ggacttaacc caacatctca cgacacgagc tgacgacagc catgcagcac
     4561 ctgtgttacg gctcccgaag gcactcctcc gtctccagag gattccgtac atgtcaagac
     4621 caggtaaggt tcttcgcgtt gcatcgaatt aatccacatc atccaccgct tgtgcgggtc
     4681 cccgtcaatt cctttgagtt ttaatcttgc gaccgtactc cccaggcggt caatttcacg
     4741 cgttagctac gctaccaagc aatcaggttg cccaacagct aattgacatc gtttagggcg
     4801 tggactacca gggtatctaa tcctgtttgc tacccacgct ttcgggcatg aacgtcagtg
     4861 ttgtcccagg aggctgcctt cgccatcggt attcctccac atctctacgc atttcactgc
     4921 tacacgtgga attctacctc cctctgacac actcgagtca cccagttcag aacgcagttc
     4981 ccgggttgag cccggggatt tcacatcctg cttaagtaac cgtctgcgcc cgctttacgc
     5041 ccagtaattc cgattaacgc tcgcacccta cgtattaccg cggctgctgg cacgtagtta
     5101 gccggtgctt attcttcagg taccgtcatc agccgctgat attagcaaca gccttttctt
     5161 ccctgacaaa agtcctttac aacccgaagg ccttcttcag acacgcggca tggctggatc
     5221 aggcttgcgc ccattgtcca aaattcccca ctgctgcctc ccgtaggagt ctgggccgtg
     5281 tctcagtccc agtgtggcgg atcatcctct cagacccgct actgatcgtc gccttggtag
     5341 gcctttaccc caccaactag ctaatcagat atcggccgct cgaatagcgc aaggcccgaa
     5401 ggtcccctgc tttctctctc aagacgtatg cggtattagc tgatctttcg atcagttatc
     5461 ccccactact cggtacgttc cgatatgtta ctcacccgtt cgccactcgc cacccaagaa
     5521 gcaagcttct ctgtgctgcc gtccgacttg catgtgtaaa gcatgccgcc agcgttcaat
     5581 ctgagccagg atcaaactct tatgttcaat ctctaacttt ttaacttctg gtctgcttca
     5641 aagaaaccaa caggacaatg ttcaaaacat tatcttgtct gtctttcaaa cagtgtgagg
     5701 ctcaaggcac tcacacttat cggtaa
//
LOCUS       DAWFIE010000102         5595 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWFIE010000102 DAWFIE010000000
VERSION     DAWFIE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.102"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(208..1719)
                     /gene="lysS"
                     /locus_tag="WGQ71_001869"
     CDS             complement(208..1719)
                     /gene="lysS"
                     /locus_tag="WGQ71_001869"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ0707826.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVNERYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTAKISYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(1716..1868)
                     /locus_tag="WGQ71_001870"
     CDS             complement(1716..1868)
                     /locus_tag="WGQ71_001870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707827.1"
                     /translation="MPSETANRFQTALPRLWKKMPTSRKIRANFVIRSGRLKQIAPDL
                     FFRKTL"
     gene            complement(1874..3127)
                     /locus_tag="WGQ71_001871"
     CDS             complement(1874..3127)
                     /locus_tag="WGQ71_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0707828.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3585..5381
                     /locus_tag="WGQ71_001872"
     CDS             3585..5381
                     /locus_tag="WGQ71_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ0707829.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFVH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSYLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVATPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
BASE COUNT         1307 a         1632 c         1438 g         1218 t
ORIGIN      
        1 ggtatttggg gcgaaaacag ccgaaaacct gtgttggggt ttcggctgtc gggagggaaa
       61 ggaattttgc aaaggtctct atttgtactg tctgcggctc gccgccttgt cctgattttt
      121 gttaattcac tatcaatcct gccatacaaa atgccgtctg aatccaatcg ggttcagacg
      181 gcattgccat ttcaactgtt tttatgatta ctcgggacgc atctgcggaa acagaatcac
      241 atcgcggatg gtttgcgaat cggtcagcag cattaccaag cggtcgatac cgatgccgca
      301 accgcctgtc ggcggcagac cgaactccat cgcgcggatg tagtcggcat cgtagtgcat
      361 cgcttcgtcg tcgcccgcgt ctttttgcgc cacttgcgct ttgaagcgtt cggcttggtc
      421 ttcggggtcg ttcaactcgg aatagccgtt tgccagttcg cggccgacaa cgaacaattc
      481 gaaacgttcg gtcagacctt gtttggtatc cgaagcgcgc gccaatgggg aaacttcgac
      541 cgggtaatcg acgatgaagg tcggattcca cagcttgccc tcggcgcaac cttcaaacag
      601 cgcgagttgc aggctgccga tgcccgggga cggcggcagg ctttcgccgt gtttgacgat
      661 ttcttttttc agccattccg catcgttcaa ctgctcgtcg gtgtagtgcg gattgtattt
      721 tttgatggct tcgaggatgg tcagacgctc aaacgggctt tccaaatcga cttctttgcc
      781 gttgtagctg attttcgccg tgccgtttac cgtgcgcgat gcgtttcgga tgatgtcttc
      841 cgccatctgc atcatacgtt cgtagtcgga gaaggcttcg taaaattcaa tcatcgtaaa
      901 ttcgggattg tggcgcaccg acatgccttc gttgcggaag ctgcgattga tttcaaacac
      961 gcgctccaaa ccgccgacca ccaagcgttt cagatacagc tcgggcgcga tgcgcaggta
     1021 gagcgggatg tccaaagcat tgtggtgggt cacgaacggt ttcgccgtcg cgccgccggg
     1081 aatcgggtgc atcatcgggg tttcgacttc gagataacgc tcgttcacca taaaattgcg
     1141 cacagattgg atgatttggc tgcgtttgat gaaggtattg cgcgattctt cgttggcaat
     1201 caaatcgacg tagcgttggc ggtatttggt ttcctgatcg ctcaagcctt tgtgtttgtc
     1261 gggcagcggg cgcagggatt tagacagcag gcggatgtcg gacacgcgta cggtcagttc
     1321 gccgtggttg gttttgaaca aagtgccttc cgcgccgacg atgtcgccca aatcccaatg
     1381 gttgaagtcg tccaaaactt cttggctcac gcctttgttg ttcagataaa gctggatttg
     1441 cccggacacg tcttgaatgg tggcaaagct cgccttgccc atttgacgct tcagcatcat
     1501 gcggccggcc actttgacgg gaatgccttg cggatcgagt tcttctttgc cgatttcgcc
     1561 gtattgggcg tgcaaatcgg cggcaaagct gtcgcgtttg aagtcgttgg gataggcgtt
     1621 gcgctgttgg cggatgttgt gcagtttttc gcggcgcagg gcgatgattt ggttttcgtc
     1681 caactgcggc tcggtttgcg gatggttttg ttcgctcata gggttttccg aaaaaataaa
     1741 tcaggcgcaa tctgtttcag acgacctgac cgaatcacaa aatttgcgcg tattttacgc
     1801 gatgtcggca tttttttcca taaacgcggc aatgccgtct gaaagcggtt tgcggtttca
     1861 gacggcatcg ttatcatttg aacattcccg ccaaattcaa taagaacaaa acggtaaaac
     1921 cggtcagata aatcaagcct gccaatgcaa gggcattcat acctgatgtg agtttgtgtt
     1981 tttcatcacc tttgaccaaa cggtaattca gccaggcaaa cacaggggcg gacacaaaag
     2041 cggcaatcat cgcaaatttg agcagattcg ccattacgcc gtcaaaccaa aaaatcaccg
     2101 ccaaaccgct gcccgccacc caaatattcc aggcaaagaa ttcggcgttg cccgttttgt
     2161 cttttccgcg cagcaggcgc acgggttcgg caatggcacg ggcatagccg tccacaacgg
     2221 taatcgtcgt gccgtacatg caggcaaacg cgataaacgc caccagcggg cgcgaccagc
     2281 cgccgatggt aacggcgtac atattgatca attgcccgat atatttgccg cccgccatct
     2341 gcactgcttc gccgttgccg tattgcacaa acgcgcccag tgcaaggaaa accaaagcca
     2401 aaaccgcact ggcgatataa ccgacgttga aatcaaaaat cccgtcgcgg tattcggaag
     2461 gattgatgcg ttgtttttcg gttacccaca aagaattgat ggcggaaatt tcaatcggcg
     2521 caggcatcca gcccatcagc gcgatcagga agcccaaacc ggcaagcgtc cacggtgtcg
     2581 gctcgataaa gtccggctgc atctgcatac cgcgcgacat agcgatgccg gcggcggcaa
     2641 gcgtggcgat actcaaagta acgatgatga ttttggaaac gcgatccaaa gcgcggtaac
     2701 gtccgctcac caaaataatc aggcaggatg ccataatcaa ggcggcaacc gtgccggcat
     2761 caaacatcag cgagggaatc gccattttga cgatggcggc ggtgacaatg gcgaccgcgc
     2821 ccgcgttaat cgtggcggag aggatgcaca aaatcaggaa tacccacaaa taaacgcggc
     2881 ttttctcggc ataaccttca atcaggctct tgcccgtgtc cagcgtgtaa tgcgcgctga
     2941 agcggaaaaa cgggtatttg aagaggttgg tcaggatgat gatgagcgcg atctgccagc
     3001 cgtaaagcgc gcccgcctgc gtcgaggcaa tcaggtgcga accgccgacc gccgccgaag
     3061 ccatcataat ccccggtccc aatgcgttga ttttactttt ccaagtcgaa atatgttgtt
     3121 cggacataaa gtcttccgta tttttaaccg tgtttcaaca cacagagccg catattcgga
     3181 cacagcccta tctattgctc caatttgggc gggattgccc ccaaacaaac ccaaatccta
     3241 ccgtcttcaa aaacaggata ccgcccggta gggaaatttt gatgaaagcg cgtattgtaa
     3301 cgtaatccaa atacctgcca acacacacta ttagaacttc atgctcaaac ttgactatat
     3361 tttccgtatt acttccaaaa aaatgcataa aacgatatct tatgcctaaa attttacaac
     3421 aaacaacctt acatcgcttt tttcgcgcaa acacgcacca tccgatcagc ccgtccgttt
     3481 tgcagcaggc tggcgatttg ataagatggt tatgtttttc agacggcatt tcagatttcc
     3541 gtccatgccg tctgaagccg caaaacccga ttggaggaac tgttatgaat accgtatcga
     3601 attacctgtc cgcattgcgc gaagccatga aggcgcaagg cttggatgca ctcgtcatcc
     3661 cttccgccga cccccacctg tccgaatacc tgcccgagca ttggcaggcg cgccgcgaat
     3721 tatcgggctt taccggctcg gtcggcacgt tcgtcctgac caccgatgaa gcgggcgtat
     3781 gggtggacag ccgctattgg gaacaagccg ccaagcagct tgcgggcagc ggcatcgaac
     3841 tgcaaaaaag cgggcaagtg ccgccgtata acgaatggct cgcggcaaac ctgcccgaaa
     3901 acgccgccgt cggcatccct tccgatatgg tctcgctcac cggcaaacgc actttggcgc
     3961 aatccctgac cgccaaaaac atccgcatcg aacacccgga cgatctgctt gaccaagtgt
     4021 ggacaagccg tcccgctatc cccgccgaaa ccgtgttcgt ccacgacccc gcctacgtct
     4081 ccgaaaccgc cgccgaaaaa ctcgcccgcg tgcgcgccgt gatggcggaa aaaggcgcgg
     4141 attaccactt ggtttcctcg ctcgacgaca tcgcctggct gaccaacctg cgcggcagcg
     4201 acgtgccttt caatcccgtt ttcgtgtcct atctattgat tggcaaagac aacgccgtcc
     4261 tgtttaccga ccgatgccgt ctgaatgccg aagccgccgc cgcgctgcaa accgcaggca
     4321 tcacggtcga gccttacgcc caagttgccg acaaactcgc gcaaatcggc ggcgcgttgc
     4381 ttatcgaacc gaacaaaacc gccgtcagca cgcttgtgcg cctgcccgaa agcgtgcgcc
     4441 ttatcgaggg aatcaacccg tccacgctgt tcaaatcctg caaatccgaa gccgacatcg
     4501 cccgcatccg cgaagcgatg gaacacgacg gcgcggcgtt gtgcggtttc ttcgccgagt
     4561 ttgaagacat catcggcaac ggcggcagcc tgaccgaaat cgacgtggac accatgcttt
     4621 atcgccaccg cagcgtgcgc ccaggcttca tttcattgag tttcgacacc atcgcaggct
     4681 tcaacgccaa cggcgcactg ccgcattaca gcgcaacgcc cgaaagccac agcaccatca
     4741 gcggcaacgg gcttttgctc atcgactccg gcgcgcaata caaaggcggc acgaccgaca
     4801 tcacccgcgt cgtccctgtc ggcacaccca ccgccgaaca aaaacgcgac aacaccctcg
     4861 ttctcaaagc ccatatcgcg cttgccgaag ccgtgttccc cgaaaacatc ccctcgccgc
     4921 tgattgatgc gatttgccgc aaacccctgt ggcaggcgca atgcgactac ggccacggca
     4981 ccggacacgg cgtaggctat ttcctcaacg tccacgaagg cccgcagcgc atcgccttcg
     5041 ccgcccccgc cacgcccgaa accgccatga aaaaaggcat ggttacctcc atcgaacccg
     5101 gactctaccg cccgggaaaa tggggcatcc gcattgaaaa ccttgccgcc aaccaagccg
     5161 tcgccacccc tcaagaaacc gaattcggca gcttcctctg ttttgaaacc ctgaccctct
     5221 gccccatcga tacacgcctg atggacaccg ccctcatgac cgacggcgaa atcgactggg
     5281 tcaaccgcta ccacgccgaa gtccgccgcc gcctcgagcc gctgaccgaa ggcgcggcaa
     5341 aagcgtggct gatcaaacgc accgaaccgc tggcgcgtta aacagcacgg cgcaaaaaat
     5401 gccgtctgaa agcccttcag acggcattgg tttcccaaaa catcccgcac cgttttcatc
     5461 ttgccgcaag caaatatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac
     5521 agtacgaata gtacggaacc gattcacttg gtgcttcagc accttagaga atcgttctct
     5581 ttgagctaag gcgcg
//
LOCUS       DAWFIE010000103         5345 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWFIE010000103 DAWFIE010000000
VERSION     DAWFIE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.103"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            215..2362
                     /locus_tag="WGQ71_001873"
     CDS             215..2362
                     /locus_tag="WGQ71_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0707830.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGGSFDLDSVDTEAPRPAPK
                     YQDISSEKPQAQKDQGGYGFAMRLKRRNWYPSAKVNEVKLNESDWEATGLPTAPKELP
                     KRQKSVIEKVETDDDSNIYSSPYLAPSNHQNGNTGNGVNQQPKNKAKDHENFQYVYSG
                     WFYKHAKQNRDLPNKIVQQGDDGYIFYHGKEPSRQLPASGTVTYKGVWHFATDTKKGQ
                     KFREIIQPSKNQGDRYSGFSGDEGEEYSNKNEPTLQSGHEGYGFTSNLQVDFDNKKLT
                     GKLIRNNANQNNTNNDKHTTQYYSLDATLKGNRFSGKAEATDKPKNGETKEHPFVSDS
                     SSLSGGFFGPKGEELGFRFLSDDKKVAVVGSAKTKDNTANGDTAAASGGAGAAASGGV
                     ADMPSENGKLTTVLDAVELTHGGTAIKNLDNFSNAAQLVVDGIMIPLLPEASESGNNQ
                     ANQGTNGGTAFTRKFDHTPKSDEKDTQAGTAANGDQATSNTAGDTNGKTKTYEVEVCC
                     SNLNYLKYGLLTRKTAGNTVGSGNGSPTAAQTDAQSMFLQGERTPVSDMAARTEANAK
                     YRGTWYGHIANDTSTSWSGNASDKEGGNRADFTVNFGEKKITGTLTAENRQQATFTIE
                     GTIQDNGFSGTAKTADLGFDLDQSNTTGTPKAYITNAKVQGGFYGPKAEEMGGWFAYP
                     GDSQTQPSASGSGASAANSATVVFGAKRQQLVQ"
     gene            2449..5187
                     /gene="tbpA"
                     /locus_tag="WGQ71_001874"
     CDS             2449..5187
                     /gene="tbpA"
                     /locus_tag="WGQ71_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ0707831.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGVSQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDTNQYAHFVVEEECPNGYAACKDKPK
                     KDIVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHTQ
                     QTFDTRDMTVPAFLTKAVFDANKKQAGSLSGNGKYAGNHKYGGLFTNGENGALVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSN
                     LRHQDYYFQNAKRAYSLKTPPQSGKNSPNGDKSKPYWVSIGGGNVVTGQICRLGNNTY
                     TDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLSWN
                     AGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRSGDKIKAVKIDPEKSFNKEAGIVFK
                     GDFGNLEASWFNNAYRDLIVRGYEAQIQNGKETAKGDPAYLNAQSARITGINILGKID
                     WNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQPE
                     GKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIKKH
                     FTLRAGVYNLLNHRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF"
BASE COUNT         1533 a         1361 c         1363 g         1088 t
ORIGIN      
        1 accgaacgag ccggattccc actttcgtgg gaatgacggg atatacgtgg gaatgacggg
       61 atataggttt ccgtgcggac gcgttcggat tcacgactgc gcgggaataa cgggattttg
      121 gtgtattccc taaaaaaata aaaaagcatt tgcaaatttg ttaaaaataa ataaaataat
      181 aatctttatc attctttaat tgaattaggt ttatatgaac aatccattgg tgaatcaggc
      241 tgctatggtg ctgccggtgt ttttgttgag tgcttgtctg ggcggaggcg gcggcagttt
      301 cgatcttgat tctgtcgata ccgaagcccc gcgtcccgcg ccaaagtatc aagatatttc
      361 ttccgaaaaa ccgcaagccc aaaaagacca aggcggatac ggttttgcga tgaggttgaa
      421 acggaggaat tggtatccgt cggcaaaagt aaacgaggtt aaactgaacg agagtgattg
      481 ggaggcgaca ggattgccga cagcacccaa ggaactccct aaacggcaaa aatcggttat
      541 tgaaaaagta gaaacagacg acgacagcaa tatttattct tccccctatc tcgcgccatc
      601 aaaccatcaa aacggcaaca ctggcaacgg tgtaaatcaa caacctaaaa ataaggcaaa
      661 agatcacgaa aatttccaat atgtttattc cggttggttt tacaaacacg ctaaacaaaa
      721 tcgtgatttg ccaaataaaa ttgtacaaca aggcgacgac ggttatatct tctatcacgg
      781 taaagaacct tcccgacaac ttcccgcttc tggaacagtt acctataaag gtgtgtggca
      841 ttttgcgacc gatacaaaaa agggtcaaaa atttcgtgaa attatccaac cttcaaaaaa
      901 tcaaggcgac aggtatagcg gattttcggg cgatgaaggc gaagaatatt ccaataaaaa
      961 tgaaccgact ttacaaagcg gtcatgaagg ttatggtttt acctcgaatt tacaagtgga
     1021 tttcgacaat aagaaattga caggtaaatt aatacgcaat aatgcgaacc aaaataatac
     1081 taataatgac aaacatacca cccaatacta cagccttgat gcgacgctta agggaaaccg
     1141 ctttagcgga aaagcggaag caaccgacaa acccaaaaac ggcgaaacca aggaacatcc
     1201 ctttgtttcc gactcgtctt ctttgagcgg cggctttttc ggcccgaagg gtgaggaatt
     1261 gggtttccgc tttttgagcg acgataaaaa agttgcggtt gtcggcagcg cgaaaaccaa
     1321 agacaacact gcaaatggcg atactgcggc ggcttcaggc ggcgcaggtg cggcagcatc
     1381 gggcggtgtg gcagatatgc cgtctgaaaa cggtaagctg accacggttt tggatgcggt
     1441 cgagctgacg cacggcggca cagcaatcaa aaatctcgac aacttcagca acgccgccca
     1501 actggttgtc gacggcatta tgattccgct cctgcccgag gcttccgaaa gtgggaacaa
     1561 tcaagccaat caaggtacaa atggcggaac agcctttacc cgcaaatttg accacacgcc
     1621 gaaaagcgat gaaaaagaca cccaagcagg tacggcggcg aatggcgatc aagccacttc
     1681 aaatacggca ggtgatacca atggcaaaac aaaaacctat gaagtcgaag tctgctgttc
     1741 caacctcaat tatctgaaat acgggttgtt gacacgcaaa accgccggca acacggtggg
     1801 aagcggcaac ggcagcccaa ccgccgccca aacggacgcg caaagtatgt tcttacaagg
     1861 cgagcgcaca ccggtatctg atatggcggc aaggacagag gcaaacgcca aatatcgagg
     1921 cacttggtac gggcatattg ccaacgacac aagcacaagc tggagcggca atgcttcaga
     1981 taaagaggga ggcaacaggg cggactttac cgtgaatttt ggcgagaaaa aaattaccgg
     2041 cacgttaacc gctgaaaaca ggcagcaggc aacctttacc attgagggaa cgattcagga
     2101 caacggtttt tccggtacgg caaaaactgc tgacttaggt tttgatctcg atcaaagcaa
     2161 taccaccggc acacctaagg catatatcac aaacgccaag gtgcagggcg gtttttacgg
     2221 gcctaaagcc gaagaaatgg gtggatggtt tgcttatccg ggcgacagtc agacgcagcc
     2281 gtccgcttcg gggtcaggcg catcagccgc caacagcgcg accgtggtat tcggtgcgaa
     2341 acgccaacag cttgtgcaat aagcacggct gccgaacaat cgagaataag gcttcagacg
     2401 gcatcgctcc ttccgatacc gtctgaaagc gaagattagg gaaacattat gcaacagcaa
     2461 catttgttcc gattaaatat tttatgcctg tcgctgatga ccgcgctgcc cgtttatgca
     2521 gaaaatgtgc aagccggaca agcacaggaa aaacagttgg ataccataca ggtaaaagcc
     2581 aaaaaacaga aaacccgccg cgataacgaa gtaaccggtt tgggcaaatt ggtcaaaacc
     2641 gccgacaccc tcagcaagga acaggtactc gatatccgcg acctgacgcg ttacgacccc
     2701 ggcatcgccg tggtcgaaca ggggcgcggc gcaagctcgg gctattcaat acgcggtatg
     2761 gataaaaacc gcgtttcctt aacggtggac ggcgtttcgc aaatacagtc ctacaccgcg
     2821 caggcggcat tgggcgggac gaggacggcg ggcagcagcg gcgcaatcaa tgaaatcgag
     2881 tatgaaaacg tcaaagctgt cgaaatcagc aaaggctcaa actcggtcga acaaggcagc
     2941 ggcgcattgg cgggctcggt cgcatttcaa accaaaaccg ccgacgatgt tatcggggaa
     3001 ggcaggcagt ggggcattca gagtaaaacc gcctattccg gcaaaaaccg ggggcttacc
     3061 caatccatcg cgctggcggg gcgcatcggc ggtgcggagg ctttgctgat ccacaccggg
     3121 cggcgcgcgg gggaaatccg cgcacacgaa gatgccggac gcggcgttca gagctttaac
     3181 aggctggtgc cggttgagga taccaatcag tacgctcatt tcgtggtcga agaagaatgc
     3241 cctaatggat atgcggcttg taaagacaaa ccgaaaaaag atattgtcgg caaagacgaa
     3301 cgtcaaacgg tttccacccg agactacacg ggtcccaacc gcttcctcgc cgatccgctt
     3361 tcatacgaaa gccgatcgtg gctgttccgc ccgggttttc gttttgaaaa caaacggcac
     3421 tacatcggcg gcatactcga acacacgcaa caaactttcg acacgcgcga tatgacggtt
     3481 ccggcattcc tgaccaaggc ggtttttgat gcaaataaaa aacaggcggg ttctttgtcc
     3541 ggcaacggca aatacgcggg caaccacaaa tacggcggac tgtttaccaa cggcgaaaac
     3601 ggtgcgctgg tgggcgcgga atacggtacg ggcgtgtttt acgacgagac gcacaccaaa
     3661 agccgctacg gtttggaata tgtctatacc aatgccgata aagacacttg ggcagattat
     3721 gcccgcctct cttacgaccg tcagggcatc ggtttggaca atcattttca gcagacgcac
     3781 tgttctgccg acggttcgga caaatattgc cgtccgagtg ccgacaagcc gttttcctac
     3841 tacaaatccg atcgcgtgat ttacggggaa agccacaggc tcttgcaggc ggcattcaaa
     3901 aaatccttcg ataccgccaa aatccgccac aacctgagcg tgaatctcgg gtttgaccgc
     3961 tttggctcta atctccgcca tcaggattat tattttcaaa acgccaaacg cgcctattcg
     4021 ttgaaaacgc cccctcaaag cggcaaaaac agccccaacg gcgacaagag caaaccctat
     4081 tgggtcagca taggcggggg aaatgtggtt acggggcaaa tctgccgctt gggcaacaat
     4141 acttatacgg actgcacgcc gcgcagcatt aacggcaaaa gctattacgc ggcagttcgg
     4201 gacaatgtcc gtttgggcag gtgggcggat gtcggcgcgg ggttgcgcta cgactaccgc
     4261 agcacgcatt cggacgacgg cagcgtttcc accggcacgc accgcaccct gtcctggaac
     4321 gcaggcatcg tcctcaaacc tgccgactgg ctggatttga cttaccgcac ttcaaccggc
     4381 ttccgcctgc cctcgtttgc ggaaatgtac ggctggcggt cgggcgataa aataaaagcc
     4441 gtcaaaatcg atccggaaaa atcgttcaac aaagaagccg gcatcgtgtt taaaggcgat
     4501 ttcggcaact tggaggcaag ttggttcaac aatgcctacc gcgatttgat tgtccggggt
     4561 tatgaagcgc aaattcaaaa cggcaaagaa acagccaaag gcgacccggc ttacctcaat
     4621 gcccaaagcg cgcggattac cggcatcaat attttgggca aaatcgattg gaacggcgta
     4681 tgggataaat tgcccgaagg ttggtattct acatttgcct ataatcgtgt ccatgtccgc
     4741 gacatcaaaa aacgcgcaga ccgcaccgat attcaatcac atctgtttga tgccatccaa
     4801 ccttcgcgct atgtcgtcgg cttgggctat gaccaaccgg aaggcaaatg gggtgtgaac
     4861 ggtatgctga cttattccaa agccaaggaa atcacagagt tgttgggcag ccgtgctttg
     4921 ctcaacggca acagccgcaa tacaaaagcc accgcgcgcc gtacccgccc ttggtatatt
     4981 gtggatgtgt ccggttatta cacgattaaa aaacacttca ccctccgtgc gggcgtgtac
     5041 aacctcctca accaccgcta tgttacttgg gaaaatgtgc ggcaaactgc cggcggcgca
     5101 gtcaaccaac acaaaaatgt cggcgtttac aaccgatatg ccgcccccgg ccgcaactac
     5161 acatttagct tggaaatgaa gttctaaacg tccaaacgcc gcaaatgccg tctgaaaggc
     5221 ttcagacggc attttttaca caattcccac catttcccat cgtccccgac aataccgtaa
     5281 tctcgaatcc cgtcattccc gcgcaggcgg gaatctagac attcaatgct aaggcaattt
     5341 atcgg
//
LOCUS       DAWFIE010000104         5014 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWFIE010000104 DAWFIE010000000
VERSION     DAWFIE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.105"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(255..1769)
                     /gene="katA"
                     /locus_tag="WGQ71_001875"
     CDS             complement(255..1769)
                     /gene="katA"
                     /locus_tag="WGQ71_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ0707832.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITQYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPASYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            complement(2032..2649)
                     /gene="tetR(B)"
                     /locus_tag="WGQ71_001876"
     CDS             complement(2032..2649)
                     /gene="tetR(B)"
                     /locus_tag="WGQ71_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HEZ0707833.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLNGFM"
     gene            2731..3936
                     /gene="tet(B)"
                     /locus_tag="WGQ71_001877"
     CDS             2731..3936
                     /gene="tet(B)"
                     /locus_tag="WGQ71_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HEZ0707834.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIVGPIIGGFAGEISPHSP
                     FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            4033..4884
                     /locus_tag="WGQ71_001878"
     CDS             4033..4884
                     /locus_tag="WGQ71_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ0707835.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVEDWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERLGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESLEIPAA
                     HKRKTAE"
BASE COUNT         1156 a         1176 c         1294 g         1388 t
ORIGIN      
        1 ggaaaagttt tcgtgccggt ttgaaccggt ggggatggtg cgggaatgac ggcggaaaag
       61 tgggctgtgg ggcgggaatg acgggatatt ttgcgtttaa taaaaaacgc ccactgaaac
      121 ggcgggggtg gggtgtggaa atgccgtctg aagcggtcaa acgacgtttc agacggcatt
      181 tttatgcccg gttatttccg gtagcggacg gcgcgggaca ggatttcttc aatttccatc
      241 cacataatgc ccccttacag caaaccgggc tggccgagtg cggggtcggt cgcgcgggcg
      301 gcttgtgcgt cttcgacagt cagtccgagg gcttttgcca cgccttcacc gtatgccgga
      361 tcgcaacggt agcagttgcg gatatggcgg tatttgatga agtcgggcgc gtcgcccatt
      421 gcggcggcgg tgttgccgaa caatgcctgt ttctgcgcgt cgttcatcag gttgaacagg
      481 gcgcgcggtt ggctgaagta gtcgtcatca tcttggcggt agtcccagtg tgccgcgtcg
      541 ccgttgattt tcaaaggcgg ttcggcgaag tcgggctgct gctgccattg tccgaagctg
      601 ttgggttcgt agtgcggcag gctgccgtag ttgccgtcgg ctctgccttg tccgtcgcgc
      661 tggttgctgt gaacagggca acgcggacgg ttgacaggga tttggcggaa gttcacgccc
      721 aagcggtagc gttgcgcgtc ggcgtaattg aacaaacgcg cctgcaacat tttatctggg
      781 ctggcgccga caccgggaac gaggttgctc ggtgcgaagg cggattgttc cacatcggcg
      841 aagaagtttt cgggattgcg gttcaactcg aattcgccca cttcaatcag cggatagtct
      901 tttttcggcc aaactttggt caagtcaaac ggatgataag gtactttttc cgcgtctgct
      961 tcaggcatga cttggatgta catcgtccat ttcggaaact cgccgcgttc gatggcttcg
     1021 tacaggtcgc gctgatggct ttcgcggtcg tcggcgatga tttttgcggc ttcttcgttg
     1081 gtcaggtttt taatgccttg ttgggtgcgg aaatgaaatt tcacccagaa acgctcgcct
     1141 gcttcgttcc agaagctgta agtatgcgaa ccgaagccgt gcatatggcg gtagctggcg
     1201 ggaataccgc ggtcgctcat gacgatggta acttggtgca acgcttcagg cagcagcgtc
     1261 cagaagtccc agttgtttgt ggcggaacgc atattggtgc gcggatcgcg tttgacggct
     1321 ttgttcaggt cagggaattt acgcgggtcg cgcaggaaga acacgggcgt gttgttgccg
     1381 accacatccc agttgccttc ttcggtataa aatttcaatg caaagcctcg gatgtcgcgt
     1441 tccgcatcgg ctgcgccgcg ttcgcctgcc acggtggtga aacgggcgaa catctcggtt
     1501 tttttgccga cttcgctgaa gattttggcg cgggtgtatt gggtgatgtc gtgcgttacg
     1561 gtaaacgtgc cgaacgcgcc cgaacctttg gcgtgcatac ggcgttcggg gatgacttcg
     1621 cgcacgaagt cggcgagttt ttcattcagc cacaaatcct gcgccagcag ggggccgcgc
     1681 ggaccggcgg tcaggctgtt ttgattgtcg gcaacaggcg cgccgttgtt catggtcaga
     1741 tgggttacgg ggcatttgga ggtagtcata gctcgtgttc cttttccttg gttaatcaaa
     1801 agatacgaca aacggcttac agtacgattt ggcggaaagc gtattcgtaa ccggtttctt
     1861 gattgcaata aatttcttga atcgacattt tgttttcctt ttgtaaaaac tatggatgag
     1921 actatacgct aagatttttg ctattaaaac tatgaaatcg atttaatatt gttataagca
     1981 atcggttttt gattttcgtt tgttttttgt tatcgaaacg aatcaaaatc cctacataaa
     2041 accattaagt tgtttttcta atccgcatat gatcaattca aggccgaata agaaggctgg
     2101 ctctgcacct tggtgatcaa ataattcgat agcttgtcgt aataatggcg gcatactatc
     2161 agtagtaggt gtttcccttt cttctttagc gacttgatgc tcttgatctt ccaatacgca
     2221 acctaaagta aaatgcccca cagcgctgag tgcatataat gcattctcta gtgaaaaacc
     2281 ttgttggcat aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg
     2341 tgtacctaaa tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt
     2401 agcgttatta cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg
     2461 gtgcctatct aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg
     2521 ccaatacaat gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc
     2581 ttcgattccg acctcattaa gcagctctaa tgcgctgtta atcactttac ttttatctaa
     2641 tctagacatc attaattcct aatttttgtt gacactctat cattgataga gttattctac
     2701 cactccctat cagtgatgga gaaaagtgaa atgaatagtt cgacaaagat cgcattggta
     2761 attacgttac tcgatgccat ggggattggc cttatcatgc cagtcttgcc aacgttatta
     2821 cgtgaattta ttgcttcgga agatatcgct aaccactttg gcgtattgct tgcactttat
     2881 gcgttaatgc aggttatctt tgctccttgg cttggaaaaa tgtctgaccg atttggtcgg
     2941 cgcccagtgc tgttgttgtc attaataggc gcatcgctgg attacttatt gctggctttt
     3001 tcaagtgcgc tttggatgct gtatttaggc cgtttgcttt cagggatcac aggagctact
     3061 ggggctgtcg cggcatcggt cattgccgat accacctcag cttctcaacg cgtgaagtgg
     3121 ttcggttggt taggggcaag ttttgggctt ggtttaatag tggggcctat tattggtggt
     3181 tttgcaggag agatttcacc gcatagtccc ttttttatcg ctgcgttgct aaatattgtc
     3241 actttccttg tggttatgtt ttggttccgt gaaaccaaaa atacacgtga taatacagat
     3301 accgaagtag gggttgagac gcaatcgaat tcggtataca tcactttatt taaaacgatg
     3361 cccattttgt tgattattta tttttcagcg caattgatag gccaaattcc cgcaacggtg
     3421 tgggtgctat ttaccgaaaa tcgttttgga tggaatagca tgatggttgg cttttcatta
     3481 gcgggtcttg gtcttttaca ctcagtattc caagcctttg tggcaggaag aatagccact
     3541 aaatggggcg aaaaaacggc agtactgctc ggatttattg cagatagtag tgcatttgcc
     3601 tttttagcgt ttatatctga aggttggtta gttttccctg ttttaatttt attggctggt
     3661 ggtgggatcg ctttacctgc attacaggga gtgatgtcta tccaaacaaa gagtcatcag
     3721 caaggtgctt tacagggatt attggtgagc cttaccaatg caaccggtgt tattggccca
     3781 ttactgtttg ctgttattta taatcattca ctaccaattt gggatggctg gatttggatt
     3841 attggtttag cgttttactg tattattatc ctgctatcga tgaccttcat gttaacccct
     3901 caagctcagg ggagtaaaca ggagacaagt gcttagttat ttcgtcacca aatgatgtta
     3961 ttccgcttat aatgatgttt cccggcatca tctgccgccg acaggccaaa agaataattc
     4021 tctcgcatta tcatgacgtt actcggaata aagctcaaac agacccagca gctcaaccag
     4081 cggctgcaac aatctttgcg cgtattgcag atgtcgggta tcgaacttga acgcgaggtc
     4141 gaagactggc tgtcggacaa ccccctgctc gaacgcaaag acacggatga attttccgat
     4201 gccgagttca gccattacac cgcgcctgcc cgtcaaatcg gcggagacga aggcgaagat
     4261 atgctgtcca acatcgccgg cgagcaggat ttcaagcaat acctgcacgc gcaagtgtgc
     4321 gaacacccgc tttccgacca agaagccgcc tgtgtccaca tccttatcga tttccttgac
     4381 gagcagggct acctgaccga cagcatcgaa gacatcctcg accatacgcc cttggagtgg
     4441 atgttggatg aagcaatgct gaaacaagcc ctgaccgcat tgaaaaaatt cgacccggca
     4501 ggcgtggccg ccgccgattt gaacgaatcg ctgatactgc agatagaaag attgggcgaa
     4561 tgtgctgcca aacccgccgc cctgcatatc gtccgaaacg ccctcgacag cattgacggc
     4621 aaccgcagcc aaaccctcgc acgaataaaa aaacacctgc cccaaaccga cagcggcaca
     4681 ctcgaagccg cactcgacct cattgcttcg ctcaatccct ttcccgccgc cggttttgcc
     4741 tcgtccacac ccacgccgta ttctgacgag gcactcgcca acctgctggc tttccgcggc
     4801 atggaggttt ctcgccgcac cattgccaaa tacagagaat cccttgagat tccggcagca
     4861 cacaaacgca aaaccgcaga ataattgctg aataatctta taaagacaac aaaccaaaag
     4921 ccggcatttc tgcgaaagcg ggaatgccga atccgtccgc gcggaaacct gcatcccgtc
     4981 attcccgcga aagagggaat ctagaaacgc aaag
//
LOCUS       DAWFIE010000105         4936 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWFIE010000105 DAWFIE010000000
VERSION     DAWFIE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.106"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(108..248)
                     /locus_tag="WGQ71_001879"
     CDS             complement(108..248)
                     /locus_tag="WGQ71_001879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707836.1"
                     /translation="MPSENLSDGIFYQSSHIFYQGCKIYPKQQKPPCRHSRKSGNLEH
                     KI"
     gene            complement(266..1204)
                     /locus_tag="WGQ71_001880"
     CDS             complement(266..1204)
                     /locus_tag="WGQ71_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0707837.1"
                     /translation="MLELNGLCKRFGGKTVADDICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIIRPDGGEIRLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAESLALSALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRNGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQNAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(1213..2088)
                     /locus_tag="WGQ71_001881"
     CDS             complement(1213..2088)
                     /locus_tag="WGQ71_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0707838.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWPDMGRRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNILFSLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNIAAGGVSSGELPSEAVCRESSEEAGLDQTLFPLIRPVSQLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVTCFEKMDIGSLVAAMLSENMMHDAQLVTLDAFCRYGLI
                     DVAHPLSEWLDGIRL"
     gene            complement(2270..2346)
                     /locus_tag="WGQ71_001882"
     tRNA            complement(2270..2346)
                     /locus_tag="WGQ71_001882"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2310..2312),aa:Met,seq:cat)
     regulatory      2496..2595
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2686..4587
                     /gene="thiC"
                     /locus_tag="WGQ71_001883"
     CDS             2686..4587
                     /gene="thiC"
                     /locus_tag="WGQ71_001883"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ0707839.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGAEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTADRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTAKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1225 a         1372 c         1282 g         1057 t
ORIGIN      
        1 tcccttaaaa cagaaaaccg aaatcagaaa cctaaaatcc cgtcattccc gcgcaggcgg
       61 gaatctaggt ctgtcggcac agaaacttgt cgggaaaaac ggtttcttta gattttatgt
      121 tctagattcc cgcttttgcg ggaatgacgg cagggtggtt tctgttgttt cggataaatt
      181 ttgcagccct gataaaaaat atggctgctt tggtaaaaaa tgccgtctga aaggttttca
      241 gacggcattt tgtttttaag aagcatcagc ggaagcggac gatttccctg tcttcgatat
      301 ggatgcgtac cgtatcgttt cccgatattt caccggcgtg cggcatatcg aggttcagcc
      361 acaggatgcc gtgttccgga tggaggacgg acaggctgaa cgattcgggc aaacaggtac
      421 gggataatac gcggcactcc atgccgtctt ggtcgaaacg caccgcattt tgcggaatat
      481 ggcggtcatc gtcggtattg ggcaaaccca tcagtcgggc gacctgcacg ccggcaggcg
      541 tttgaaccaa ggtttcgggc gtaccgcatt gaaggatttt cccctcgtgc atgacggcga
      601 tttcgtccgc cgccgtgcag gcctcttcgg gcgaatgcgt taccaaaacg gcagggatgc
      661 cgccgtttcg gatacgttcg gcagtcatgc ggcgcagccg gtcgcgcaaa tgcgtgtcca
      721 aactggaaaa cgattcgtcc agcaacagca aggaagggcg gacaaccaaa gcgcgcgcca
      781 gtgccaaccg ttgcttctcg cctccggaaa gtttttcagg cttgcggtgc gcctcatttt
      841 ccagtccgac ttcggcaagt gccgacaagg cgaggctttc ggcttcggct ttcggcattt
      901 tttgcatttt caaaccgaat gccgcatttt ccagcgcact catatgggga aacagcgcgt
      961 aatcttgaaa catcagcgaa atacggcgtt tttcgggcgg catacgggta atgttttccc
     1021 cgttcagccg aatttcgccg ccgtccggac ggataatccc cgcaatcata ttcagcagtg
     1081 ttgattttcc gcagcccgac cgccccaaaa cggcgagtat tttgccgcgc ccgacagtca
     1141 ggcagatatc gtcggcaacc gttttgccgc cgaagcgttt gcagagtccg ttcagttcaa
     1201 gcatggcgca tcctataaac gtatgccgtc cagccactcg gacagcggat gggcgacatc
     1261 aatcagaccg taacggcaaa acgcgtccag cgtaaccagt tgcgcgtcgt gcatcatgtt
     1321 ttccgacaac atggcggcaa ccaggctgcc gatgtccatt ttctcaaaac acgtaacctc
     1381 gccgtcctga ttttcaggca ggaaggtttc gggcaggacg gcatcgaaca catacaggat
     1441 ttcattgtgc acaccccggc tgacggggcg caggctgtgc agctgcgata ccgggcggat
     1501 gagcggaaac agcgtttgat ccaaacccgc ttcttcgctg ctttcgcgac acacggcttc
     1561 agacggcaat tcaccgctgg aaacaccgcc ggcggcaata ttgtcgagtt tgtcgggatc
     1621 gactgctttg tgcggactgc gcctgcctat ccaaaaatgc catctgccat ccgattcagt
     1681 cagaccgttg agatggacgg cgcggctgag tagtccgaac gggcggaaag cggcgcgttc
     1741 gagtgagaac aagatattgc cgccgccgtc ggtcaggtcg aaacactcgt tgcgccatcc
     1801 gtgaagcagc cccgccttgt tccatgtgcg ggcgaggtgc tgcaagcgtc tgcccatatc
     1861 tggccagccg tccgcattca ggaaaatgcc gtctgaagac tccgagcagc ctgcctccca
     1921 gtcttttttg acgcgctccg cccattccgg cgacagattg cccaaaggca gacggttcag
     1981 atacagcgtt ttccagcaac tttctgcacc gtaacttgct tttgcccact cgaacagggc
     2041 atcaaggtcg tgtttgctga cggattcggt aaaacggacg gtcggcatag tcggctttct
     2101 tgaagacaat gcggaagatt gtagccaaac tgcctgaatt tgcaaccgca aacagacggc
     2161 ggtctgccgt tccgatattg cggcaggaag gaaaatccgg caaaaaatgg aggcggcagg
     2221 aaacaggcaa aacaaaaccc ctcggcattg aaccgagggg tttgatattt ggttgcggga
     2281 gcaggattcg aacctacgac cttcgggtta tgagcccgac gagctaccat gctgctccat
     2341 cccgcgtcag aagatgaaac tatacggcag attttttatg ttgtcaaaca ttacttccgc
     2401 gaaaaatcat aaaattttgc cgtccggcgg tttatgcctg atttgaaata ttattttctt
     2461 tacaaaagtt catgttcgtg atttaatttt ggttaacatt gaaacagggg tgctgcctga
     2521 tgttcaggcg gctgagaaat accctttaca cccgatcggg ataatacctg cgtggggagt
     2581 tttcacggat tctgtctttc agacagcatt ggttttcaaa tgccgtctga aaacgcaaaa
     2641 cgctcctgtt tctttattct aaacgagaaa acaggagcat tttttatgac tacgccaaaa
     2701 aaaaccgcca aaacttccgg caacgaagcg cgcgaacttg ccgacttgag cgaagacatc
     2761 ggtatccgct tcaaatatcc gaactcggaa cgcgtgtatc tgcaaggcag ccgcgacgac
     2821 atccgcgtgc ctttgcgtga aatccgtcag gacgacacct atacggcgca aggcgcggaa
     2881 gccaacccgc cgattcccgt ctatgacacc agcggcgtgt acggcgatcc agcggcacac
     2941 atcgacctga aacaaggtct gccgcacatc cgcaccgcat ggctgaacga acgcggcgat
     3001 accgaaatcc tgcccaagct ctccagcgaa tacggcatcg aacgcgcaca cgatccgaaa
     3061 accgcccatc tgcgcttcaa ccaaatcacc cgcccgcgcc gcgcgaaaag cggtagcaac
     3121 gtaacccagc ttcactacgc gcgccaaggc attatcacgc ccgaaatgga gtttgtcgcc
     3181 atacgcgaac gtttaaaatt agacgaattg tcccaaaaat cggaatacgc caaactcttg
     3241 aaacaacacg cagggcaaag tttcggcgcg aacatcccaa cccgtcccga ccaaatcacg
     3301 cccgaattcg tgcgccaaga aatcgccgcc ggacgcgcga ttatccccgc caacatcaac
     3361 caccccgaac tcgaaccgat gattatcggc cgcaacttcc gcgtcaaaat caacggcaac
     3421 ttgggcaatt ccgccgtcac ttccagcctg accgaagaag tcgaaaaaat ggtgtggtcg
     3481 ctgcgttggg gcgcggacac aattatggac ttatccacag gcgcgcacat ccatgaaacg
     3541 cgcgaatgga ttatccgcaa cgcgcccgtc cccatcggca ccgtgccgat ttaccaagcg
     3601 ttggaaaaaa ccggcggcat tgccgaagat ttgacttggg atttgttccg cgacacctta
     3661 atcgagcagg cggaacaagg cgtggactat ttcaccatac acgcaggcgt gttgctgcgt
     3721 tatgtgccga tgactgccga ccgcctcacc ggcatcgtat cgcgcggcgg ttcgattatg
     3781 gcgaaatggt gtttggcaca tcatcgggaa aacttccttt acacgcattt cgacgaaatc
     3841 tgcgaaatta tgaaggcgta tgacgtatcg ttcagcctcg gcgacggttt gcgtcccggc
     3901 tgcattgccg atgccaacga cgaatcccaa ttcgccgagc tgcacacctt gggcgaattg
     3961 accgccaaag cgtggaaaca cgacgtacag gtgatgatcg aaggtcccgg ccatgtaccg
     4021 ctgcaacgcg tcaaagaaaa tatgaccgaa gagttgcaac actgttttga agcaccgttt
     4081 tacaccctcg gcccgctcgt taccgacatc gcccccggct acgaccacat cacctcgggc
     4141 ataggcgctg ccaatatcgg ctggtacggc acggcgatgc tctgttacgt tacccccaaa
     4201 gaacatttgg gactgcccga caaagaagac gtgcgtaccg gcatcatcac ctacaaactc
     4261 gccgcccacg ccgccgacct cgccaaaggc tggccgggcg cacagttgcg cgacaacgcc
     4321 ctgagcaagg cgcgtttcga gttccgctgg cgcgaccagt tccgcctaag cctcgacccc
     4381 gaacgcgccg aaagcttcca cgacgaaacc ctgcccgccg aaggcgcgaa aatcgcccac
     4441 ttctgttcga tgtgcggccc caaattctgc tcgatgaaaa tcacgcagga agtgcgcgac
     4501 tacgccgaca agcaaaaagc ccaacggcag ggcatggagg aaaaagcggt cgagttcgtc
     4561 aaaaaaggag cgaagattta cagctaaaac gtcaagcaaa aaatgccgtc tgaaacccgg
     4621 aaaaaaggct tcagacggca ttctttcgct tgtgaaaata tagtggatta acaaaaatca
     4681 ggacaaggcg acgaagccgc agacagtaca gatagtacgg aaccgactca cttggtgctt
     4741 cagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgccgta ctggtttttg
     4801 ttaatccact ataattttta aaatttttat attcacatag atggggatga tgggatttag
     4861 ggttctgatt ttgtttttga gagaatgaag gaatttgaga ttgtgggcga ttatcgggaa
     4921 aaatagaatc tttccg
//
LOCUS       DAWFIE010000106         4935 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWFIE010000106 DAWFIE010000000
VERSION     DAWFIE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.107"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(650..1186)
                     /locus_tag="WGQ71_001884"
     CDS             complement(650..1186)
                     /locus_tag="WGQ71_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ0707840.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEGKVVLVRQWRYAANQATLELPAGKLDVASEDMAACALRELAEETPYTAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(1220..1576)
                     /gene="folB"
                     /locus_tag="WGQ71_001885"
     CDS             complement(1220..1576)
                     /gene="folB"
                     /locus_tag="WGQ71_001885"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ0707841.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1632..2234
                     /gene="plsY"
                     /locus_tag="WGQ71_001886"
     CDS             1632..2234
                     /gene="plsY"
                     /locus_tag="WGQ71_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ0707842.1"
                     /translation="MFNISAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIWATLLIALLVLFRHKSNIVKLLEGRESKIGGSR"
     gene            2311..3156
                     /locus_tag="WGQ71_001887"
     CDS             2311..3156
                     /locus_tag="WGQ71_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ0707843.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSKAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPKLYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLKNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3402..4376
                     /locus_tag="WGQ71_001888"
     CDS             3402..4376
                     /locus_tag="WGQ71_001888"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ0707844.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDTFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPAKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVDYLNRLHSK"
     gene            complement(4534..4752)
                     /locus_tag="WGQ71_001889"
     CDS             complement(4534..4752)
                     /locus_tag="WGQ71_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ0707845.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         1287 a         1452 c         1014 g         1182 t
ORIGIN      
        1 aagtattcat atattctatc tttaattatt tcattatttt cttcattttc ttcatctaaa
       61 aatatacatc tttttaatag ttccccatta aattcataaa catcgtaata agctttaaaa
      121 atagatatca tatcatttcc ttatcttcca taaatcggct tcgcttgttt aaatactgcc
      181 tgcgccaatt accgttctca aattcaaatt gggcttttag gtctataaca aagaaaaagc
      241 cttggcagga taaacctacc aaggcttttt attaaaaaaa acgctctatt actgtgtcta
      301 aaaatgcaaa aaaagtaacc atcataaccc ttttgccaaa atcaatatca tcatccgatg
      361 aatgaattga cggtatataa gaaagaatta ataagctcat aaaccggtaa gaaaaatcag
      421 taacaaagat attcattatt aactattctt aaaaatgatc acgttcgttt gggtaattat
      481 cagcccatac cataccctac tcctattact aaagcacgaa tgactttaaa gggcgcaccc
      541 gacggcaagc cgaacccttg cctccccgaa gccgaaatca gaaaaaccat accccatccg
      601 ccaaacaaaa tgcccgccaa tctactccgg cgggcagttc aacaccctgt caatccttca
      661 tcagccagta ttgcaaaccg attaatgtct tgccgtcttt aatttcatcg tttgccaatg
      721 cctgacggac ttcttctttc gacatcaata cggtttccgt aatctcgtct tcgtcattgg
      781 caagcgtact gcccaaacgc acgccttccg cctcgaacag atacattttt tcgttgcaaa
      841 aaccgaccgc cgtataaaaa ctgtaaagca ggcgtacgct gtcggcggta taaggcgttt
      901 cttcagccag ttctcgtaaa gcgcatgctg ccatatcctc gctcgccaca tccagcttgc
      961 ccgcaggaag ttccaatgtc gcctgatttg ccgcataacg ccactgccgc accaaaacca
     1021 ctttcccctc gtccgtaacc gccaacacgc atgccgcacc cggatggcgg atgacgatac
     1081 gctgcccttc attgccgttg ggcaacctga ccttatccct gctgatgctg acgaaaccgc
     1141 cctcgtaaat ggtttcgccg cctaatttta cttccctcaa atccacttca tatctccttt
     1201 ccgtttccta ttctgccgtt caatcttcac gcttgccgcg ctcgatttcc acgccaacct
     1261 cgcgcacgcc ttccaaaata cccggcttga cgattttcac gcgcacccac accgcgccga
     1321 aatatcccaa aaccaaatcg gcaatatatt ccgccaacgc ttccaaaagc aggaaatcct
     1381 gttctttcag atgtcggcgc agcgtttcgc atacctcggc ataatgcacc gtattggcaa
     1441 tatcgtcgtc cgaacccgct ttctcgggaa caccgatgtc caaatcgaca atcagggtct
     1501 gcaaccgttc gcgctcccag ccgtacacgc cgataagcgt atccgccttc ataccgtgca
     1561 aaaagatttt atccataatc cgccccgttt tttgctaaaa taaccgcacc attctaagta
     1621 aagtaccgca aatgttcaac atatcggctg ttgctgtttc ctatctgatc ggctcacttt
     1681 ctttcgccgt catcgtgtcc aaatattacg gcatggacga cccgcgcacc tacggatcgg
     1741 gcaatccggg ggcaaccaat gttttacgca gcggcaaaaa aaaggcggcc gcgctgacgc
     1801 tcttgggcga tgccgccaaa ggtttagttg ccgttttgct tgcacgcgtg cttcaagaac
     1861 cgctcggttt atccgacagc gcaatcgcgg ccgtcgcact cgccgcgctg gtcgggcata
     1921 tgtggccggt gtttttcgga tttaaaggcg gcaaaggcgt ggcaacggca ttgggcgtgc
     1981 ttctggcact ctctcctgca actgccttgg tctgcgcgtt gatttggctt gtgatggcat
     2041 tcggcttcaa agtatcctcc cttgccgcat taaccgccac aatcgccgcc ccccttgccg
     2101 cactgttttt tatgccgcat acttcttgga tttgggcgac actccttatc gccctgctgg
     2161 tgttgttccg ccacaaaagt aatatcgtca agctgctcga aggcagagaa agcaaaatcg
     2221 gcggcagccg ctgatttccc atccatccct gtttatgccg tctgaaccac gcttcagacg
     2281 gcatccgaat atcccgccgt tttaccgact atgctcagtt acagacacgc attccacgcc
     2341 ggcaaccacg ccgatatgct caaacacttt accttgtttt tagtgctgca atatttcaac
     2401 cgcaaagaca agccctactg gtacatcgac acgcacggcg gcgcgggtgt gtacaatctc
     2461 gaaggcagca aagcacagaa agtcggtgaa taccgccaag gcatcgccct gctccgacag
     2521 gcgcaaaacc tgcctgccga actctctgac tttgccgcgc acatacaaaa aatcctgccc
     2581 tcgcccaaac tttactgcgg ttctccgtgg ctggcgcaat cgctgacccg tgtcggcgac
     2641 aaattgcgcc tgtttgagct gcaccccacc gattttgtcc atctgaaaaa caatatgggc
     2701 gaagcaggat tgggcaaacg tggacaagta ttgcgcgaag acggttacaa agggctgatt
     2761 tccctgctgc cgcccccgcc gcgccgcgcc accgtcctca tcgacccgcc ctatgaggaa
     2821 aaacaggact accggcgtgt aacggaaacg ctgaaagccg ctttaaaacg gtttgagtcc
     2881 ggctgttatc tcatttggta tccctgcctc agccgcgaag aaagccgcaa gctgcccgaa
     2941 gaattgaaaa aactcctgcc cgacaactac cttcacgccg aacttcacgt ccacgcgccg
     3001 aaagccgacg gtttcggtat gcacggcagc ggaatgttcg tcatcaatcc gccctatctg
     3061 ttggcggaac aattggcggc aaacctcccc gccctgacgc ggctgctggc gcaagacgaa
     3121 ggcgcgcgct accttttgga cagcaaaatc cgctgacccc gatgccgtct gaaacccttc
     3181 agacaacatc tcccgtcaaa taaacaaaac cggacgatat accggcagtc ggcactttta
     3241 agaataaata tcccctcatc cgcctgccgc gttccgactt gccccatgcc gtttttttac
     3301 actacaatcc cctagactat cctgcaaaac gcaccaaagg aatacgccac aataacctgc
     3361 cgcctttcag acggcacaaa cctttccgaa acgagcaaac tatggacaca ctgacccgat
     3421 tcctccccga acacctgcaa caaaaccagc tgcccgaagc actcggcggc gttttactgt
     3481 ccgttgtttc cgcctgcacc gaaatcaacg ccaaagtccg cctcggcgca ctggccggcg
     3541 tattgggtat ggcgggcacg ggcaatattc agggcgaaga ccaaaaaaaa ctggatgtta
     3601 tcgccaacaa catcatgatt gacacactca aagccaaccc tgccgttgcc ggtttggcaa
     3661 gcgaggaaga agacactttc gtaagtgccg gtgaaaacgg acgctatctc gtcctattcg
     3721 accctttaga cggatcgtcc aatattgatg tcaacatttc cgtcggtacg attttctcca
     3781 tccttgaaaa acccgaaggc gcattggcaa ccgaatcatt ccttcaaacg ggtagacagc
     3841 agcttgccgc aggctatgtc ctctacgggc ctcaaaccca gctcgtattc acattcggac
     3901 acggcgtata tatgttcacg ctcaatgccg aaaacgaatt tgtgctgaca aaagaaaaac
     3961 cgaaagtacc cgaaagtacc aaagaatttg ccatcaatat gtccaaccgc cgccactggc
     4021 tgccccccgt ccaacaatac atcgacgagc tcttggcggg cgaaaccggc acgcgcggca
     4081 aaaactacaa tatgcgctgg gtagccagca tggttgccga aatccaccgc atcctgatgc
     4141 gcggcggcgt gttcatgtat ccgcaagaca aacgcgaccc cgccaagccc ggcaaactgc
     4201 gcctgatgta cgaagccaac cctatgagcc tgatactcga acaggcgggc gcatcggcaa
     4261 gcaacgcata tcaagccatg ctcgacatac agcccgaaaa cctgcaccaa cgcgtcgccg
     4321 tcttcatggg cagcagcgaa gaagtggatt acctcaaccg cctgcattca aaataaaagg
     4381 cagcctgacc gataaagaaa ggcggcagcg cgcatccgac gctgccgccg atatttcgtt
     4441 tatagcctta aaaagataga aaaccacctt tccgaacggc aaaaatgccg tctgaaaagt
     4501 tttcagacgg catttcaaaa actcaaaccc gaattatttt cccgctgctt tttgcaggat
     4561 ggcgtacaac tcgtctttca gtttcaattt ggcttttttc agctcatcga tggtttccgc
     4621 accgctggta accggattgt tgaccagacc agtaatttta tcgtccagct cgttgtgttc
     4681 gtcgaacaga cgggcgaagc gggaattttc ctgtttcaat ttggaaatca aatcacggta
     4741 ttctgggaac atatcgcttt cctctcttgg tttacggtta aaaagtcaaa tgaatcttta
     4801 cattaattat aacaaagata atatttttat ccagtgtttt atcggatacg gcaaaatttt
     4861 cttttaacac cgccgtccgg ttgataaaaa cgttgacaat atcacgcttt acgcataaat
     4921 tttcagcctt tccta
//
LOCUS       DAWFIE010000107         4813 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWFIE010000107 DAWFIE010000000
VERSION     DAWFIE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.108"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(550..795)
                     /locus_tag="WGQ71_001890"
     CDS             complement(550..795)
                     /locus_tag="WGQ71_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707846.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            907..1335
                     /locus_tag="WGQ71_001891"
     CDS             907..1335
                     /locus_tag="WGQ71_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ0707847.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            1347..1823
                     /locus_tag="WGQ71_001892"
     CDS             1347..1823
                     /locus_tag="WGQ71_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ0707848.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISELSRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(2173..2661)
                     /locus_tag="WGQ71_001893"
     CDS             complement(2173..2661)
                     /locus_tag="WGQ71_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ0707849.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAGRTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(2729..3784)
                     /gene="aroG"
                     /locus_tag="WGQ71_001894"
     CDS             complement(2729..3784)
                     /gene="aroG"
                     /locus_tag="WGQ71_001894"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ0707850.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            complement(3946..4173)
                     /locus_tag="WGQ71_001895"
     CDS             complement(3946..4173)
                     /locus_tag="WGQ71_001895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707851.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            4400..4708
                     /locus_tag="WGQ71_001896"
     CDS             4400..4708
                     /locus_tag="WGQ71_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707852.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKSK
                     "
BASE COUNT         1190 a         1220 c         1130 g         1273 t
ORIGIN      
        1 ttaatgcggc aagaatttat cggaaaaaac tgaagtttaa agacctagat tcccgcctgc
       61 gcgggaatga cgatttagaa atcacccgaa acgagaaaaa gaaccgaaac cggacaagtc
      121 ggattcccgc acgggaggga aggacggaat ttcagattgt tgtttttgat tttctgtttt
      181 tgtgggaata acgggacttt ggatgatgtg catttatcga gaaaactaaa aaccctccgc
      241 cgtcattccc gcgaaagcgg gaatctagaa ttttaatgcc gcaagaattt atcggaaaaa
      301 ctgaagttta aagacctaga ttcccgcctg cgcgggaatg acggtttaga aatcacccga
      361 aacgagaaaa agaaccgaaa ccgaacaagc cggattaccg cctgcgcggg aatgacggct
      421 caaaagttac gaaacgaaaa ataaccgaaa ccaaacaagt cggattcccg cgcgggtggg
      481 aatgacgata agcgtaattt caatctgcca tattgtcttt tcacttgcct gaaaacagat
      541 ttcagccacc tatccctgca tccggcggct gatgtagtcg gcaatcaaat cccgcttttc
      601 ccaaaaaaac ttctgcattt tttgttcgga atacagtttg cggaccagag tgggcaaacc
      661 gctgtggacg atggcggcga tttctttgtt gcctttttct tcctgcattg ccgtctgaaa
      721 atccataccc ctgcctttca gatattctgc aaggcggttg cggatgtggg aattgagttc
      781 gatggatttg agcattttgc ctccgttttg cggtaaggat ttattcgatt tgtttatccg
      841 cctgatttta tcacgtttcc caagcggttt gcctgtacaa taccgctttt cggcagggtg
      901 tgggcgatga tgttttcttg gttcaagctg tttcacttgt tttttgtcat ttcgtggttt
      961 gcagggctgt tttacctgcc gaggattttc gtcaatatgg cgatgattga tgtgccgcgc
     1021 ggcaatcccg agtatgtgcg tctgtcgggc atggcggtgc ggctgtaccg ttttatgtcg
     1081 ccgttgggct tcggcgcggt cgtgttcggc gcggcgatac cgtttgccgc cggctggtgg
     1141 ggcagcggct gggtacacgt caaactgtgt ttgggcttga tgctcttggc ttaccagttg
     1201 tattgcggcg tgctgctgcg ccgttttcag gattacagca atgctttttc acaccgctgg
     1261 taccgcgtgt tcaacgaaat ccccgtgctg ctgatggttg ccgcgctgta tctggtcgtg
     1321 ttcaaaccgt tttgacggag catcttatgc cgatagaaat cgaacgccgt tttttgattg
     1381 gaaacgacaa ctggcggcaa tacgccgacg aaccgctgct gttgaggcag ggttatctgt
     1441 ctgttgaaaa agaacgcacc gtccgtgtcc gtattgccgg acaacgggcg tggctgacgc
     1501 tgaaaggcta tatttcggaa ctcagccgca gtgagtttga atacgaaatc ccgcttgccg
     1561 atgcggaaaa gatgatggaa acgatgtgtc cgtttaaaat ggaaaaatgg cgttatccgg
     1621 tcagatgggg cggcagcctg tttgaaatcg atgtttttct tggcgataat gcgcctttgg
     1681 tcgtcgcgga aatcgagttg cccgacgaga atgccgattt tgaccgtccg gactggctgg
     1741 gacgggaaat cactgccgac ggtatgttta ccaatgctta tttgagcagg catccgttct
     1801 caagctggaa aaatgccgtc tgaaacgact tcagacggca ttctccgaca accatcgcaa
     1861 atccaaagac agcctctata tccttatttc tgctttcttt gttccattat tcccaataaa
     1921 tccccataac ctaaaatctc gtcattcccg cgcaggcggg aatccggttc tttaaacttc
     1981 ggttttttcc gataaattgc tttagctttt cgtttccgga ttcccgcttt tgcgggaatg
     2041 acgatggaag attgttgtta tttcggataa attaccctaa ctttaaaatc ccggattccc
     2101 atcttcgggg gaataacaat attttctatt tcagcccatc atttgcgggg gattttatag
     2161 agagtttata tttcaatatc tcaaataatg cacaaaagca tagcgcgtgc ctttgctgct
     2221 gacgcggcgt tccgtccgcc ctgcctcttt ccaatgcgtc cggtctattt cggggaaaaa
     2281 tgcatctcct tccacagaca aatccacttc ggttatccgc aaatcggtcg ccaatggcat
     2341 cgcttgtccg tatatctgcg cgccgcccat aatgacagct tcttccgcgc ctgcgcacaa
     2401 tgccaatgcc gcctccaaac ttgccgccgt ttccgcgcct gccgcgcaat aatccgcctg
     2461 ccggctgatg acgatgttcc tccgtccggg caggggtttg acgggcaggg attcccacgt
     2521 tttccgcccc ataatgacgg gtttgcccaa ggtataggct ttgaaaaatg cgaaatcttc
     2581 ggggatgtgc caaggcatag cattgcccgc cccgatgcac aggttttccg cacacgccgc
     2641 aattagggtt attttgagca tatttcctcc acaaaaatgc cgtctgaacg cttcagacgg
     2701 cattttatga cattccgtgt caaaaatctc aaccggcgcg cgccatacgt tttttgtttg
     2761 cacctgccaa caatgccaac agttcttcag tcgcgcccca accgatacac gcatcggtaa
     2821 tgctcttgcc gtacacttcc ggcttgtcct gcctgccttc gaccaaatgg ctttccacca
     2881 tcacgcccat gatattgctc ccgtcctgtt ccaattgggc agcaatatct tgtgccactt
     2941 ccatctgccg ggtgtaatcc ttgcggctgt tggcgtggct gcaatcgatc atcagcttgt
     3001 cggttacccc tgccgcacgc agttgttccg ccgcctcgct gacgtgtccc gcatcataat
     3061 tcggctcttt tccaccgcgc aaaatgacat gacagtcggg attgccgccg gtatggacaa
     3121 tggcggaatg cccggccttg gttacagaca ggaaatgatg cgaatggctc gccgcaccga
     3181 ttgcgtcgat ggcaatcttc aaattgccgt ccgtaccgtt tttaaagccg acggggcagg
     3241 acagcccgct tgccaattcg cggtgaactt ggctttcggt cgtccgcgcg ccgattgccc
     3301 cccaagaaat caagtccgca taatattgcg gcgtaatcat atccaaaaac tcggtagagg
     3361 caggcatacc catattgttc aacgacaaca acaggctgcg cgcctgacgc aaaccgaaat
     3421 tgatgtcaaa cgtaccgtcc aaatgcgggt cgttaatcaa acctttccaa cccaccgtcg
     3481 ttctcggctt ctcgaaataa acgcgcatca caatcaaaag ctcgttttca tactgcttgc
     3541 ggagtttcaa caaacgctcc gcatattcca atgccgcttt cggatcgtga atcgaacacg
     3601 gtccgataat aaccaacagc cgcttgtccc tgccgtgaac caaatcggaa atttcctgac
     3661 gggtgcggtg aaccaagccc gaagcctctt tggaaatcgg cagctcgtaa agatgggcaa
     3721 tcggcggcaa caactcttta acttctttaa tcttaatatc gtctgtgggg taatggtgtg
     3781 tcatcttgca tctttcctga tgggttttta tacgcggctt aggttacatc gtttaccttg
     3841 cacaaacaag tgcaacatat tactacaaag ctattttatt tcaaaataac tgatatatct
     3901 tttttcatag attttaaatt tcatattttt aatattttag gaattttatt ccaaagaaaa
     3961 agaaacagat tgataaaaac aatacccacc attcattcgg acaaaaccgc acggaacatc
     4021 cgttttccca tttccacgac aatccggtac agaacacccc gctgccgatt ccgcctgcac
     4081 atccggcaaa atttcatacg caaggaaaat gttgaaaaca aaaagaacta attgcttgaa
     4141 aaaagattta attggaatgc gggtatttcc cacactttct ccatttattc atcctcatta
     4201 taggaagaaa aacatgaaaa aaccctgctt gcactctcac ttgcagccgt gtccgccgtt
     4261 acggtagccg atattcctga gtctaacggc aagggcaaag ccatcatcgg cgataaggaa
     4321 aaacaccgtt accaagcatg aaaaccttgt tttgaaagtc tatggcgaaa tccgccaaga
     4381 tttgggggac ggatgatcct tgaccccttg ggcggatgca tatgcagatt tgcgcggcaa
     4441 aaccaaagtg atgacgaccc agatgtgtgc ttcccgcgat gtcagcaaaa gcgccaaagg
     4501 ttggagtgtc ggtatcggtc tgaatgtagg caaacagttg accgacagcg tcggtctcga
     4561 gtttgatcca tactaccgtc acaaaacaat ctgcaaaccc cgtgagattg ttttggacgg
     4621 cgacaaaacc aaaatgggcc gctccaaatc caacgagtac ggcttccgcg taaccgcaac
     4681 gttctatagt caattaaaat caaagtagga cagtagtgca tcgtcaaatc gggcgtaatc
     4741 agacaaaacg gttcgcagat accgcttaat attcgcccac actttctcaa tcgggttgag
     4801 ctcaggtgaa taa
//
LOCUS       DAWFIE010000108         4759 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWFIE010000108 DAWFIE010000000
VERSION     DAWFIE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.109"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..466)
                     /locus_tag="WGQ71_001897"
     CDS             complement(<1..466)
                     /locus_tag="WGQ71_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0707853.1"
                     /translation="MKIVLTTSMAGLGGTETATVRLGRLLKRRGHDIILASSDGPFVG
                     EAQASGIRWQPVDFYRGGLAGYLKSTFAYARMLRREQPDIIDCQMARVVPACALAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPADR"
     gene            complement(463..1884)
                     /locus_tag="WGQ71_001898"
     CDS             complement(463..1884)
                     /locus_tag="WGQ71_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ0707854.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVSKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEENAPPARLSATAESASALLAAAVCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            complement(2079..3188)
                     /gene="ribD"
                     /locus_tag="WGQ71_001899"
     CDS             complement(2079..3188)
                     /gene="ribD"
                     /locus_tag="WGQ71_001899"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ0707855.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAQDSQIVGQG
                     FHVKAGEPHAEVHALHQAGEMAKGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLESAGIKTECGLLENKARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERDEDKLHPYRKHAHIRILMPSETAGGK
                     IDLNHLMRLLADEGFGEIMVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLLSLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNDNAF"
     gene            complement(3220..3684)
                     /gene="nrdR"
                     /locus_tag="WGQ71_001900"
     CDS             complement(3220..3684)
                     /gene="nrdR"
                     /locus_tag="WGQ71_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ0707856.1"
                     /translation="MKCPFCAHPDTRVADSRLMDERNAVRRRRYCPNCGKRFGTLETA
                     ELKMPAVIGTDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETARLTELRLYASGLRD
                     ISAAELVEIVLEELFGQSTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            complement(3714..4556)
                     /locus_tag="WGQ71_001901"
     CDS             complement(3714..4556)
                     /locus_tag="WGQ71_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ0707857.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDETWAQLAVDELVMFREGKIILNDRPRHPVYMSVEEGLETARAA
                     GVAV"
BASE COUNT          964 a         1221 c         1600 g          974 t
ORIGIN      
        1 tccggtcggc ggggaaaccg tggcggatga gtttttcgcg ttcgtgtttg cagttaccga
       61 tgatgtacac gcccagcttg tcgaagagtt tggcgatttt ggggtaggtt gccgcgtcca
      121 aaccgtgcga gtggtagatg attttggttt tcggcgaaac gattttggcg gcaagggcgc
      181 aggctggaac gacgcgcgcc atttggcagt cgatgatgtc gggttgttcg cggcgcagca
      241 tccgggcgta ggcaaaggtg cttttgaggt agccggcaag cccgccccgg taaaaatcaa
      301 ccggctgcca acggatgccc gatgcttgcg cctctccgac aaaaggcccg tccgaagaag
      361 caaggatgat gtcgtgcccg cgccgtttga gcagccgccc gaggcggacg gtggcggttt
      421 ccgtgccgcc caagcctgcc atagatgtgg tcaaaacgat tttcataatg ggaaaccttg
      481 ttttttcaaa taatgaaaca gtttgtgcaa atctttccgg tggcgcagga tgcagcctgc
      541 cagatatgcc gcccatacgc cggcaaacag ggggtagttt gccggtgtgc cgaagcaggt
      601 gtaggccgcc gaggaggcca ggcagaacaa tgtgtgcata taaagcggca ggcgtttgag
      661 cggctgccac aggcgacagg agctttctgt cttgaaaaca aaaaacaacc agaatgaggc
      721 ggcacaggca accgccgcgc cgcgcgcgcc gccggacggt acggcaagcc ccagcagcag
      781 caggtttgcc gccagcgcgc ccaaggtggc gagcgcgatc gggcgtgttt ttcggacgac
      841 gttcaaaccg atgccgctga tttctaccag cgtgcaaaac agcggcggca gcatacacga
      901 tacgacgata aaccggacgg cggcgtagtt ttccggcagc aggagggagg cgaggggcga
      961 gaaaatgccg gtcaggcaga cggcggcggc aagcagggcg gaggcggatt ctgccgttgc
     1021 cgagaggcgg gcgggcgggg cgttttcttc gattgcgcgg aaaatatacg gtgtccagac
     1081 cgttgaaaag atgctttgga acaataatgc cgccccgccg aacgaaatac ccatcgaata
     1141 aacgccgagc tgttctaggc cggcatattt tttcaggaac aaacggtcgg cggatgccag
     1201 cccccaatag gcgatgctgc ttagtgcgat cggtatgccg tagcgcaggc cgcgatgcag
     1261 gacggcggat gaaaacggtg cgcgccggac ggccttcaga cggcatcggt tttgaaacag
     1321 caaaaaggcg gcggcggcaa ggtttgccag cgcgtaaacg gcggtcagga cggcggtgtt
     1381 cgccggaaag tgcagcagcc cgaccgtcag cggcagcagc agcaggatgg cgagcttgga
     1441 cacgagttgc gcggacgaaa aggcaagggc gcgtccttcc atacgcaaaa ccagtaagag
     1501 aaagcggatg ggcaggaagc tcagttcaaa cagcaccagc ccgatgccgg cggcggcatc
     1561 gtcgagcgaa aacaggattt cagacggcag ggacgggcgg gaaagcagca gggcggctat
     1621 cgcggcggca gacagcagcg gcggcaggaa cagggttttg aacaaggtgt ctttgtcggc
     1681 ggcggcatag tattcgcgga cgtatgcctg atccagcccg aggcacaata ccgacaccgt
     1741 cagtcccgcc gccgtctgca tcagcacgat gcgcccgatg tcgtcggcgg ggaaatacca
     1801 cgacagcagc ggcaggatga tgacggctaa aaccgcgctg ccgatcgagc ctgccgcgta
     1861 gccgaggatt tcttttgtgt ccatttttga tgtccgggcg gcggcgggat gctgcctgtg
     1921 ccgtctgaag cctttcttga tcggaatttg acggctttca gtccgttgcg gctgccggcg
     1981 gggtgcggca gtccgggttg cgcttttccg ggcgggcggc ggtctgaacg gggccgtttt
     2041 ttatcggcgt tattatatag tgaaacggcg caaacccttt aaaaggcgtt atcgtttttc
     2101 cggaacacgg ttttgatgtc gtgtccgagg atttcgcttg aaacgggtgt ccacaagggc
     2161 ggtgcggaaa gggcggcgcg gttttcgggc agggaaagca ggtctttgcc gctgccgagg
     2221 attttgggcg aacggtacag cacgatttcg tccgccagat cttctgccag aaatgcggat
     2281 gtgagttcgg agcctgcttc gaccatgatt tctccgaaac cttcgtcggc aaggaggcgc
     2341 atcaggtggt tcaggtcgat tttgccgcct gccgtttcag acggcatcag gatgcggatg
     2401 tgtgcgtgtt tgcgataggg gtggagtttg tcttcatcgc gttccaaggt ggcgatgtag
     2461 gttggagatt gtccgtcggt cagaaggtgg ctgtccaagg gcaggcgcag gcggctgtct
     2521 aaaacgatgc gtgcgggttg gcgcaaagtt ggaaaagcgc ggacgttgag ccggggattg
     2581 tccgccaaca ccgtgccgat gccggtcagc accgcgcagc tttcggcacg caaaacctgt
     2641 acgtcggcac gcgcgtcttc gccggtaatc caaaagctgc tgccgtctga aagggcggtt
     2701 ttgccgtcca gcgaaacggc gcatttgagg cggacgaagg gacggcggcg ttcgatgcgc
     2761 gataaaaatc cccggttgag ttcgcgtgct ttgttttcaa gcagtccgca ttctgttttg
     2821 atgcctgccg attcgagcag ggcaagccct ttgccggcaa ccagcgggtt ggggtcgcgc
     2881 atggcggcaa cgacgcgtgt tacgccggaa cggagcagag cttcggcgca gggcggtgtg
     2941 cgcccgtaat ggctgcacgg ttcgagggta acaaaggcgg tcgcgccttt tgccatttcc
     3001 cccgcctgat gcagggcgtg gacttcggcg tgcggttcgc ccgctttgac gtggaagccc
     3061 tgcccgacaa tttggctgtc ttgtgcgata acgcagccga cgcgcggatt gggcgaagtg
     3121 gaaaaacgcc ccaaagcggc aagtcggagg gcgttttcca tcatggatat atctgtgtcc
     3181 gaaaacatgg ggataccgta tcagtatggg ttgggggaat caggctttgc cgcctgtttt
     3241 gacggcttgc gccagccacg aggcaaagtc tgccggattg tcgaagcgtt tgtgcagggc
     3301 ggcgaaacgg acggcggctt ccgtgctttg cccgaacagc tcttccagta cgatttcgac
     3361 aagttcggca gcggatatgt cgcgcaaacc cgaagcatac agcctgagtt ccgtgaggcg
     3421 ggcggtttcg tcgatctgtt cgggtgtcag ggcggatttt cgggcggcgg cggtcaggtc
     3481 gttgcggagg cgttgtgcat tgaagggcga acgttttttg tccgtgccga tgacggcggg
     3541 cattttgagt tcggcggttt cgagtgtgcc gaagcgtttg ccgcagttgg ggcagtatcg
     3601 gcggcggcgg acggcgttgc gttcgtccat caggcgcgaa tcggcaacgc gggtgtcggg
     3661 gtgggcgcaa aacgggcatt tcatcgggtt cgtcctccta tgtcgtctga agttcagacg
     3721 gcgacgccgg cggcgcgcgc ggtttccaaa ccttcttcga cactcatata aacggggtgc
     3781 cggggtcggt cgttcaaaat aattttgcct tcgcggaaca tcaccagttc gtcaacggca
     3841 agttgcgccc acgtttcatc gcgggtcagc ggcagggtgg cgataacggc gacgcggtcg
     3901 gagggcgtgg ttacttcggc aaaatcgacc atcacgtcgt cgtcgagcag gcgcgccttg
     3961 ccgaacgggg cttggcggac gatgtagtgc agcagtgtgc tggcgtgggc aaacagggca
     4021 atgccgtctg aaagcataaa gttaaacagc ccgaacttgc ggatttcgtg cgtcagcccc
     4081 gcaatcgcgt caaacagcgt gtcgtcgtcg ggacgggcgg caaagcgggt gcgcaggcgg
     4141 ttgaggatgt ggcagaacgc gcgttcggaa tcggttgtgc cgacggggtg gaaaaattcg
     4201 ccctgttcgg ggaaaaaatc aatcaaatgt ccgttgtggg caaacagcca gtagccgccc
     4261 cacatttcgc gcataaaggg atgggtgttc gccagcgagg tttgtccttg tgatgctttg
     4321 cggatatggg cgacgacgtt ttccgattta atctggtagg cgcgcacgag gtcggcgacg
     4381 ggcgaattgg cactgggttt gtcgtcatgg aacaggcgca cgcctttgcc ttcgaaaaag
     4441 ccgatgccga aaccgtcggc atggtggtcg gtaatgccgc ccctgcggcg gaagccttca
     4501 aaggaaaaca taatatcggt cggcgtattg cagttcatgc ccagcagttg acacatggtt
     4561 tgtcccaatg attcagatgg tcgcaagtat tcggattata ccccgaactg aaaatgccgt
     4621 ctgaaatacg gcttgttccc cgttattccc tcgaaaacag aaaacagaaa acaaagacgg
     4681 aaacttaaga ttccgtcatt cccgcgcagg cgggaatccg acttgtccgg ttttggttgt
     4741 ttttcgttcc gtaactttt
//
LOCUS       DAWFIE010000109         4611 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWFIE010000109 DAWFIE010000000
VERSION     DAWFIE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.110"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(45..3104)
                     /locus_tag="WGQ71_001902"
     CDS             complement(45..3104)
                     /locus_tag="WGQ71_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEZ0707858.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDR
                     SIAEGLMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKV
                     RVSDTPMEGLQIIGLDDPVLQRTYSHMFDADKEAFSESADYGFEPYFEKQHPSAFSAV
                     KAENARNAPFRRHAGQGKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKAT
                     VSAEARISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEP
                     AAPDAWVVEPPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPSGFEQVQRSRIAETDHLA
                     DDVLNGGWQEETAAIADDGSEGVAERSSGQYLSETEAFGHDSQAVCPFENVPSERPSC
                     RVSDTEADEGAFQSEETDAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLA
                     EFKVKVKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGK
                     TCMGLELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLV
                     AGTTGSGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMK
                     LAANALNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLMPDDPEP
                     LEKLPFIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGL
                     IKANIPTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDE
                     EVHRVVEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKA
                     SISGVQRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3269..3949
                     /locus_tag="WGQ71_001903"
     CDS             3269..3949
                     /locus_tag="WGQ71_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ0707859.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLDRYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAALWLAALLMLPYCLLFA
                     GSVGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRSIWR"
     gene            3978..4337
                     /locus_tag="WGQ71_001904"
     CDS             3978..4337
                     /locus_tag="WGQ71_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ0707860.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALSNIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            complement(4469..>4611)
                     /locus_tag="WGQ71_001905"
     CDS             complement(4469..>4611)
                     /locus_tag="WGQ71_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707861.1"
                     /translation="NEFDEEDGWPDLSCSLHTFKTTLIAWNAFLQLPKSIHSVVETVI
                     EE"
BASE COUNT          963 a         1337 c         1151 g         1160 t
ORIGIN      
        1 gccgtctgaa acagtcttca gacggcattt ccatttgcaa aaaatcaagc attgtccaag
       61 gggacgagaa tcgtacggtt gccgttgtgt tccggtgcgg acacaatgcc ttccgcctcc
      121 atctggtcaa tcagacgcgc ggcgcggttg tagccgatgc gcaaggcgcg ctgtacgccc
      181 gaaatactgg ctttgcgcgt tttcaggaca acggatacag cttcatcata catcggatcg
      241 gtttcgccgt cgccgctgcg cccgatgccg ggcagctctt cgctgccgcc gccgctcaaa
      301 atatcgtcaa catagtccgg ctcgccaaac tgtttcaaat attcgaccac gcggtgcacc
      361 tcttcatccg aggcaaacgc gccgtgaacg cgctgcggat aggcagtacc cggcggcagg
      421 aacagcatat cgccctgacc gagcaggttt tccgcgccca tttggtcgag aatcgtgcgg
      481 ctgtcgattt tgctggacac ttggaacgcg atacgcgtcg ggatgttcgc cttaatcaga
      541 cccgtgatga catcgacact ggggcgttgt gtggcaagaa tcaaatggat gcctgccgcg
      601 cgggcttttt gggcgaggcg ggcaatcagt tcttcgattt tcttgcctgc cgtcatcatc
      661 aggtcggcaa actcatcgac cacgaccacg ataaacggca gtttttccaa aggttcggga
      721 tcgtcgggca tgaggctgaa cggattgccg attttttctc cccttgctgc ggcttcggcg
      781 attttttggt tgaagcccgc aagattgcgt acgcccataa agctcatcag gcggtagcgt
      841 ttttccattt cgttaacaca ccagttcagc gcgtttgccg ccagcttcat atcggtaacg
      901 acaggggcga gcaggtgcgg gatgccttcg taaatgctca attccagcat tttcggatcg
      961 atcataatca tacgcacgtc ttccggcgcg gctttgaaaa gcatagacag aatcatcgcg
     1021 ttgacaccca ccgatttgcc cgaaccggtc gtgccggcaa ccaacaaatg cggtgctttg
     1081 cccaagtcgg ttacgacggg ctgtccggtg atgtcctgac cgagcgcgag cgtcagcttg
     1141 gatttggatt cggcaaactc gggcgaattg aagatttcgc tcaggcgtat catttggcgt
     1201 ttcgggttcg gaagttccaa acccatacag gttttgccgg ggatggtttc gacaacgcgg
     1261 atggaagcca cgccgagcga acgcgccaag tctttttcca gatttagaac ggaattgccg
     1321 cgcacgccga catcgggttc gatttcataa cgcgtaatca cggggccgga ataagaatcg
     1381 acaaccttga ccttgacttt gaactccgcc aatttttctt cgatggtgat gctgttttcc
     1441 aacagttctt cttcggtttg cgtcgcctcg ggattgaaca gcggcggcag aagcaggtcg
     1501 gttgtcggca ggtgttcgga taccgcatcg gtttcttcag attggaacgc cccttcatcc
     1561 gcttccgtat ccgatacccg gcaggacggg cgttcagacg gcacattttc aaacggacaa
     1621 accgcctgac tgtcatgccc gaacgcttcg gtttccgaca aatattgccc gcttgaccgc
     1681 tctgccacac cctcactgcc gtcatccgca atagcggcgg tttcctcctg ccaacctcca
     1741 ttcaaaacat catcggcaag atgatcggtt tcggcaatgc ggctgcgttg cacctgctcg
     1801 aatcctgacg gcggttcata ggtacggttg tagatttccg atacgggagg cggcggcgga
     1861 atatcgattg cgggcatggg aactttcggc acttcgggtg gttcgaccac ccaagcatcg
     1921 ggcgcggcag gttcttcaat atggatatcg gcagtttcat cataaaccgg accgccgtat
     1981 cccacagacg aaacggtttc cgtgaaaaca ttttcggttt cagacggcat ttcgacatcc
     2041 cgtttcccga caaccgtccg actttccgga atcaggcgcg aaatccgcgc ctccgcagaa
     2101 accgttgcct tgttcggttc tttcaaattg acggaaacgc ggcggcgggc atcgcggacg
     2161 gctgtgccgt ctgaaacgga ctgcccttgg gaaacatccg gggattttgc ctccgcctgc
     2221 cctttcccct gccctgcatg acggcggaac ggcgcattcc gtgcattttc ggctttgact
     2281 gcagaaaagg cagacggatg ctgcttctca aaatacggct caaatccgta atccgcagac
     2341 tcggaaaacg cttctttgtc cgcatcgaac atatgggaat acgtgcgttg aagcacaggg
     2401 tcgtccaaac cgataatctg cagcccttcc atcggggtat cggacacgcg gactttgggg
     2461 accgctgttt cgtttttctc aaatgcatcg atatagcgtt ttttcgtttc ccttaaagcc
     2521 gcgctgttta aagcacgcgt tgcttcttcc aaagtgattt ccttaaaaac cggacggacg
     2581 ggcgaaattt cgctttcaga cggcatcaac ccttcagcaa tactccggtc gaatgggatg
     2641 cggcggttgt cgattacggc agttgcaatg tcttccgtat cggcagcctc ttcctccgca
     2701 gcttctgctt cttccgtttc cgcttcttcc gtttccgctt cttccgtccc gtttccactg
     2761 tctgaatatc cgtcggatgc ggtcggaaca tccgcacgat ttgcagaagc aggcggaacg
     2821 ggcctgtccg gttcggattc gtgcgccaga tagtttcggc agaaacggac gacaccgaca
     2881 aacagccaat acactgccgt tttgacggca tggaaaagca tcagggcaag ttcgggaaaa
     2941 tcgggcatac cgtctttgat ttcaggcagc tccgcctgtt tttccccttt cttttcctgc
     3001 cacgcagaaa cctcgcgcat ccactcgcgt tcggattgtg cgcggacaaa aaacaagccg
     3061 gcaagcgcaa gcaacaaaat aacgatcaaa actatccaaa acatatgccg tttcccgcaa
     3121 gacaaacgat taaacgggtt tattttaacg tgtttccatc ccaaaaaaaa caaccggtcg
     3181 gcaattctgc cgaaaccgat tcggctttcg gtttgccccc gccgacagta taatagccgt
     3241 cccttccctt aatctttgcg gcattgccat gattttccaa acagtctggt tttcagatat
     3301 ggtactgtcg gtttcgtgga ttgtccttat cctgatactg gcagcttccg cgccgtccgc
     3361 attccgctcg cttgaccgct accgttccgc ccttccactg tgtaccgtaa ttttttccgc
     3421 agcatggtgt ctgaacgcct ctgccggcgg cggacagctt gcacagatga actaccacct
     3481 gctcgccgtc aatctggttg cactgatggt gggcacttcc gccgcccttt ggcttgccgc
     3541 gctgctgatg ctgccctact gcctgctgtt tgccggttct gtcggcgcat atccgccgaa
     3601 cgctttggtg ctgattctgc ccgcgctggt cgtcaaccgc ctgtcgcgta tgctggtcaa
     3661 ccgcctgccg cccaatattt tcatctttat tttcgtcaac ggctttctcg cttccgccgc
     3721 cggcattctg ctgaccgggc tggtgctgac cggtattttg gatgccgcaa acgcttttcc
     3781 gtccgaaata ttgtggacga ccgccctgcc cgtctttatt ctgctggcgt gggcggaagc
     3841 cttcctcagc ggcatttcaa ctgccatatt tgttgcactg aagccccatt ggatcaacac
     3901 tttcgacgac aaccgctatc tgaagtccga ccgtagcata tggaggtagc cttcagacgg
     3961 cataggcaga caaaaccatg ctttccaaca tcatccccct ttccatcggt gcggcactcg
     4021 gtgcaacggc gcgttggctg ctcaacctcg ccgttcctgc cgccatgtct cccgccacgg
     4081 gcaacctgtt tgccaactgg acgggcgcgc tgctcatcgg catctttgcc gaaaccgtca
     4141 atcacccgca atggaagctg ctgctgatta ccggttttct cggcagcctg accacgcttt
     4201 ccggattttc actggaaacc gtaaccctgc tccaatcgaa tcgtcccgct tcagcacttt
     4261 ccaatatttt cctacacacg gcaggttcgc tgctgctgac ttggctcggg ctgaagatag
     4321 ggacggcagt caaataatca aaaaaattgc ttcctttggt tttgacacaa actatacaag
     4381 tcctaacatc ttaatcatgg cgcagcatat aagcgtggat ttgcagccca atctatactt
     4441 tctgattcta ttcattaagc cattatgctt attcctcaat cacggtttcc accactgaat
     4501 gaatactttt aggcaattgc aaaaaagcat tccaagcaat taaagtagtt ttaaaagtat
     4561 gcagcgaaca gcttaaatca ggccagccgt cttcttcatc aaattcgttg t
//
LOCUS       DAWFIE010000110         4563 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWFIE010000110 DAWFIE010000000
VERSION     DAWFIE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.111"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(193..1548)
                     /locus_tag="WGQ71_001906"
     CDS             complement(193..1548)
                     /locus_tag="WGQ71_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ0707862.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETVKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(1563..1835)
                     /locus_tag="WGQ71_001907"
     CDS             complement(1563..1835)
                     /locus_tag="WGQ71_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ0707863.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENQINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(1961..2698)
                     /locus_tag="WGQ71_001908"
     CDS             complement(1961..2698)
                     /locus_tag="WGQ71_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ0707864.1"
                     /translation="MKKLSRIVFSIVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(2967..3866)
                     /gene="prmB"
                     /locus_tag="WGQ71_001909"
     CDS             complement(2967..3866)
                     /gene="prmB"
                     /locus_tag="WGQ71_001909"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0707865.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGNGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            4011..>4563
                     /locus_tag="WGQ71_001910"
     CDS             4011..>4563
                     /locus_tag="WGQ71_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707866.1"
                     /translation="MVDGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     KNSGHELKVTWQGVKHAASKANQAELSIITKLDDLLRYAKYEGSYSDRKGRPDIIAAH
                     KYRAVAKVGNESLNIGVIVREFPD"
BASE COUNT         1258 a         1178 c          972 g         1155 t
ORIGIN      
        1 agttcaatga gtttgttttg tttatactgg cttagacgac tttttctcat agggataatt
       61 ctaacttaat ttgaatttcc ctagttatct aggacagccc catattcttt tctaaagaca
      121 ttttacccga tgcctataaa caaaaatgcc gtctgaagcc tttcagacgg catttcccca
      181 tcaaaaccgc aatcagtttt tcatcgattg aaccggtgcg ggaatcctgc cgccccggtt
      241 gacgaacact tcgcacgaac cttctttgac cggcattaca ggcgcgtagc ccaacaggcc
      301 gccgaactcg acgctgtcgc cgacggtttt acccgttacc ggaataatgc gcacggcagt
      361 ggttttgctg ttaatcatac cgatggcggc ttcgtcggca atgatgccgg aaatggtgtg
      421 cgcgggtgtg tcgccgggaa cggcaatcat atccaagccg accgaacaaa cggcggtcat
      481 cgcttcgagt ttatccaacg tcagcacgcc tgcttcggcg gcggcaatca taccttcgtc
      541 ttcggaaacg gggataaacg cgccactcaa accgccaacc gcgctcgaag ccatcatgcc
      601 gccctttttc acggcatcgt tcagcaatgc caaagctgct gttgtgccgt gcgtaccgca
      661 gacgctcaaa cccatttctt caagaatgcg cgccaccgag tcgccgacgg caggggtcgg
      721 tgccagcgac aagtcgagaa taccaaacgg gatattcagc atttttgagg cttcgcggcc
      781 gatgagttcg cccacgcggg taattttgaa ggcggttttc ttcacaactt ccgcaacttc
      841 ggtcaatgtc gttgcatctg aattttccaa cgcggctttt acgacacctg ggccggatac
      901 gccgacatta atcacagcat ccgcttcgcc cgaaccatga aacgcgcccg ccataaacgg
      961 gttgtcttcc accgcgttgc agaacacgac aattttagcg cagccgaaac cttcgggcgt
     1021 gatttccgcc gtgcgtttga cggtttcgcc cgccagcttg accgcatcca tattgatacc
     1081 ggcacgcgta ctgccgatat tgatggagct gcacacaata tcggtagtct tcatcgcttc
     1141 gggaatggag cggattaaca cctcatccga aggcgacatc cctttttgca ccaacgcgga
     1201 aaagccgccg ataaaggaca cgccgatggc tttggcagcc ttatccaaag tttgcgccac
     1261 gctgacatag gaatcggcat gggtagccgc cgcgatttgg gcaatcggcg tgacagaaat
     1321 gcgctgattc acaatcggca ctccatattt ggcagacaga tattttgccg tcgccaccaa
     1381 gtctttgccg accgtggtga ttttgttgta aatgttttgg tttaacacgt cgatgtcggt
     1441 gctgatgcag tcgtgcaaat caatgccgat ggtaatggtg cggacatcaa aattctggtc
     1501 ggcaaccatt ttgacggttt ctaaaatttc gccggattgg atactcatca cattcctcca
     1561 actcaaatgc ggtgcatcgc ttggaagatt tcttcgtttt gcatacggat atcaagcgcg
     1621 agttttttgc tctcttccgc aaacaaatcc aaaacctctt gacgcgattt gctgcatttt
     1681 gaagtgtcca ccaagataat catagtaaaa aaatcgtcca tcagctgttg gctgatgttg
     1741 agaatattga tttggttttc cgccaaaatt ttggaaacat cgtacacgat gccgacgcgg
     1801 tctttaccga tgacggtgat gactgaattg ttcacaggct tactccttgc agatatccgt
     1861 taaagtccga aattatacca ccgttggatt ttgaagaaat attgtcaaca atatatacat
     1921 acaaaatgcc gtctgaaact atttcagaca gcatcaagat tcagggttcg attaaataac
     1981 catccttatc ccactgggtt ttcctgacca acttgtcatc ctgataaaca gcctcgctct
     2041 ttttagaacc atcttcatac cactccaaaa ccaccccgtt gcgttgatgg tggcggatag
     2101 acagttccga gagtaatcgg ccgctttcat cccaagtcag aattttggca ggctcatcgt
     2161 tgaccataac catttccgtc ttgatactgc cgtcggcata ccattgcttc catacgccgt
     2221 ttgccttatt ttgcttaaac tggatttcgc tttccttgcc gccgttacgg taatagcggt
     2281 accccgtacc ttcactcaaa ccatttttat aaggcataac ggcagatttt ttaccgttcg
     2341 gataccagtt gacccactcc ccgtccggct tacccttgct gaagcccccc gccatttttt
     2401 tctgaccatt aaaatgccac aaaatcaaca taccgttttg cagggtaggc acaaaagatt
     2461 tgatttgcgt tgaagcaacg atataaggtt cggaatattt cttcatcgac ggataataaa
     2521 aatcctgcgc gtgtgcgata cccgccgcca cactatattg cctgatataa gcggcagaag
     2581 acatcgtcgc cgtcagtttc ccgttctgat taaaataaac agaataggtc tgcgccggca
     2641 aagcggccga aaaacccaac aggacaattg aaaatacaat ccgagataat tttttcattg
     2701 caatagcgat ataaaaacaa ggctgtgttt tagtaatctg ttgatttcaa ttaaagacaa
     2761 ttattttccg gttaggaata aacctattct attgaataga ttgaagccaa gtacgcctat
     2821 caacactata ttaaaacact gccaaaaaca attaacttat aaacaatatg gtaaggattt
     2881 ctctgccaag catcaaaccc gagacaacgt atcgtaaaaa tgccgtctga aaacaaatcg
     2941 tcttcagacg gcatttcccc ttcaactcac tcttcaccca ataactgctc gcgcgtcaag
     3001 aggaaaacaa aaccgtcgcc cccgctggtt tccaaccaag taaaaggcaa ctccggatac
     3061 gctgcttcca atacatccct gttatgcccg atttccacca gcaatacacc tttgggattc
     3121 agaaactttg ccgcattcag aagaatctgc ctggtggcat ccaacccgtc cgccccgctg
     3181 cccaatgcca attccggttc gtgcaaatac tcttcaggca ataactcaac cgattccgca
     3241 tccacataag gcggattgga aacaatcaaa tcataagtgc cttccaatcc ttcaaacaaa
     3301 tccgtatgaa taagccggat gcgttcttcc aaaccataat cttcgacatt aatccctgcc
     3361 acttccaaag catccaagct cacatcaacc gcatcaattt gggcatcagg ataatgatgc
     3421 gccatctgaa tggcaaggca accgcttccg gtgcaaagat ccaaagcatt atgcaccagc
     3481 tcatcgtatt ctatccaagg acgaagtccg ttacccaaca attcataaat aaaagaacga
     3541 ggtatgatta cgcgctcatc cacatagaaa tcaaactctc cctgccatgc ctggtgtgtc
     3601 aaataagcgg ctggaatgtg ttcgacagca cgacgctcaa taaccgccag cacttcctct
     3661 ttttcagctt ccaagagttt tgcatcaaga tatggggcaa gcatatccaa aggcaaattc
     3721 aaagtatgca gaatcaaata agccgcttca tcatgcgcat tatctgttcc atgaccaaaa
     3781 aagagccctg cctcattaaa acggctgact gcaaaacgta aaatatcgcg gatagtcgtc
     3841 aattcttgtg ctgcctgatt aaacataata tgaaccattc tgcgtataga tacttttaat
     3901 tataacagaa acaacaagca aaccttttca tatcgccaaa taaccaccca atctacccat
     3961 acaactacat aaatgcccgc gcgaaaacca tcgcccgaac ggaaacgaca atggtcgacg
     4021 gcatgggcaa tctgattggc tgggaaaaaa cggggcttgt tgtcggtaag cagtggataa
     4081 ccgcaaaaga cgacaaggtg tccgatgtct gcaatgccaa cggcgagatg ggcgtaatcg
     4141 ggctttacga gcctttctca cacggcgcat tgacgatacc cggtcatccg aactgccgat
     4201 gcgaggttgt ttccgtatcg ggtggcgaat tgggagaatt tgccgaaaaa aaggagcttc
     4261 gtaaagcggc tatgcagtat gcgcgggata actttatcgg caaaagctat gtcaataaaa
     4321 acagcgggca tgaactgaag gtaacttggc aaggtgtgaa acacgctgcg tcaaaggcaa
     4381 atcaggcgga attatccatc ataacaaaac ttgatgactt attgcgctac gcaaaatatg
     4441 agggttctta ttcggatagg aaaggtcgtc ctgatattat tgcagcacat aagtatcgtg
     4501 ccgttgccaa ggttgggaat gagtctttaa atatcggcgt gattgtaagg gaatttccag
     4561 acg
//
LOCUS       DAWFIE010000111         4522 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWFIE010000111 DAWFIE010000000
VERSION     DAWFIE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.112"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..941)
                     /locus_tag="WGQ71_001911"
     CDS             complement(<1..941)
                     /locus_tag="WGQ71_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0707867.1"
                     /translation="MYSNNAITNNRNNITLGAYRDAIISIFSDENVVRAIGTINSMYE
                     EYLTRSHKIDQKEFLREGERLSAKAREFQRTAEEAQKSLKEGIGRAASKFRGNSKEAF
                     VARKVQSDLQKALNASNYNKQQYARRAATALENASKSKVMAANLGKFTYGATVAMGTL
                     SMGSAWWKALDGEGNWSDVGLSSASFLGGLMGVQWGAALGVGAFGLLTGGAGWLVVGA
                     ILGGGAGAWWGSLGAEKAWRWFEDFLNLNRSDKYHVYDPLALDLDGDGIETVATKGFA
                     GALFDHRNQGIRTATGWVSADDGLLVRDLNGNGIIDNG"
     gene            2215..4347
                     /locus_tag="WGQ71_001912"
     CDS             2215..4347
                     /locus_tag="WGQ71_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ0707868.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPIKRLAMATLPALVWCDDGNHFILAKTDGEGEHAQFLIQ
                     DLVTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
BASE COUNT         1157 a          998 c         1035 g         1332 t
ORIGIN      
        1 ccgttgtcga tgatgccgtt gccgttcaaa tcgcggacga gtaaaccgtc atcggcagaa
       61 acccaaccgg tggcggtgcg gatgccttga ttgcggtggt cgaacaatgc acctgcaaag
      121 cctttggtgg caacggtttc tataccgtcg ccgtctagat ctagggcgag ggggtcgtaa
      181 acgtggtatt tgtcgctgcg gttaagattt aagaaatcct caaaccatct ccaagctttt
      241 tcagctccta atgatcccca ccaagctcct gcgccacctc caagtatagc tccaactact
      301 agccaacctg cgccaccagt aagtaaacca aaagccccaa cacccaaagc cgcaccccat
      361 tggacgccca ttagcccacc caaaaaagaa gctgatgaga gaccaacatc actccaattt
      421 ccttctccat ccaaggcttt ccaccaagca cttcccatac tcaaagttcc catggcaaca
      481 gttgccccat aagtaaactt acccaaattc gctgccataa cttttgattt tgaagcattc
      541 tctaacgctg ttgccgcacg tcttgcatat tgctgcttgt tatagttgct agcattcaat
      601 gctttttgca aatcactttg tacttttctt gcaacaaatg cctcctttga attaccacga
      661 aatttactag cagctcttcc aattccttct tttaagcttt tttgtgcctc ttctgctgtg
      721 cgttggaatt ctcttgcctt agcagacaga cgctcaccct cccttaaaaa ttctttttga
      781 tctatcttgt gcgaacgggt caaatattct tcgtacattg agttaatcgt accaatcgcc
      841 ctaaccacat tttcatcaga aaatatggaa ataatagcat ccctatacgc acctagtgta
      901 atattgtttc tattattagt tatagcatta ttcgaataca taatagcacc tccaaattta
      961 gaactgaata cgtttatatt ctctgtgtaa taatatcaaa taattaatta atggataagt
     1021 aagtccgaaa aaataacaac taaaataagc aatatactga ttctctcctt tattaattag
     1081 atatattgaa aaacaaataa aaaataaaca gaaaacattc atgtgaaaaa gccacctcat
     1141 attcttattt ataagtttaa aataactagg cattgtatat ttaaaaatcg gtacatccaa
     1201 cggaaacaaa ccacataaaa atttttcttt attctttatt aaagacatag ttttattttt
     1261 caaaattagt ccagataata tactaacaat tactattaaa acaataagca cagcgtaatt
     1321 ttttataaaa ttcattaaaa taataacgaa aatactaaat agattacata ctataaattg
     1381 aaatataaaa tattcagtat ttaatccatt ttcaaccgcg agttttttaa aatatccttg
     1441 ctcgaaaaaa ttaaaaaaat ccattaatac tttcctctca agaaaactaa ttttagttta
     1501 gttaagataa aatgcaatat taccttttct ccataaaagt atggagctta tatacataaa
     1561 ttatgtgcga ggtagcctga aaaatatcca atacaagata gtaggagatg tttcccttat
     1621 cactattggc caatacccaa ttggcaggca ctctatttcc aaaattttct caagaatagt
     1681 taaatactat taattagttt aaaaacaata acatacgttt gtatttatcc gttttggtct
     1741 gcctgaaaca gactctatgt ccatacacgg acatgtagac agtttccttg ttattttggt
     1801 tgctcgtcat tagtgatgat actttactcc tctcttaaaa aatcgtcaag aactgttatc
     1861 aatctttaaa gtctttttta aacaaatata tatacgttca ggctgtctga aacacaaaaa
     1921 aatgatgtta cccgaccgtg acagagtcgg ggcagccggt accttaaccc tgcaccttaa
     1981 ccctgtgcct taacggtata taggaggcac gataggtgcg aggcatccgc atctatcttg
     2041 attcctaagc cgttttacct tcctgaaacg agaacttttc catactttcc ctttacaatt
     2101 cctgtataga tccttacaat gtgttctgct gttttattta tatatttctt tctacttaat
     2161 atattccttt tccagccagc cgttatccac atccaatcta actatatttt atttatgtct
     2221 atcgtatccg caccgctccc cgccctttcc gccctcatca tcctcgccca ttaccacggc
     2281 attgccgcca atcctgccga tatacagcat gaattttgta cttccgcaca gagcgattta
     2341 aatgaaacgc aatggctgtt agccgccaaa tctttgggat tgaaggcaaa ggtagtccgc
     2401 cagcctatta aacgtttggc tatggcgact ttacccgcat tggtatggtg tgatgacggc
     2461 aaccatttca ttttggccaa aacagacggt gagggtgagc atgcccaatt tttgatacag
     2521 gatttggtta cgaataagtc tgcggtattg tcttttgccg aattttctaa cagatattcg
     2581 ggcaaactga tattggttgc ttcccgcgct tcggtattgg gcagtttggc aaagtttgac
     2641 tttacctggt ttattccggc ggtaatcaaa taccgccggt tgttttttga agtattggtg
     2701 gtgtcggtgg tgttgcagct gtttgcgctg attacgcctc tgtttttcca agtggtgatg
     2761 gacaaggtgc tggtacatcg gggattctct actttggatg tggtgtcggt ggctttgttg
     2821 gtggtgtcgc tgtttgagat tgtgttgggc ggtttgcgga cgtatctgtt tgcacatacg
     2881 acttcacgta ttgatgtgga attgggcgcg cgtttgttcc ggcatctgct ttccctgcct
     2941 ttatcctatt tcgagcacag acgagtgggt gatacggtgg ctcgggtgcg ggaattggag
     3001 cagattcgca atttcctgac cggtcaggcg ctgacttcgg tgttggattt ggcgttttcg
     3061 tttatctttc tggcggtgat gtggtattac agctccactc tgacttgggt ggtattggct
     3121 tcgttgcctg cctatgcgtt ttggtcggca tttatcagtc cgatactgcg gacgcgtctg
     3181 aacgataagt tcgcgcgcaa tgcagacaac cagtcgtttt tagtagaaag catcactgcg
     3241 gtgggtacgg taaaggcgat ggcggtggag ccgcagatga cgcagcgttg ggacaatcag
     3301 ttggcggctt atgtggcttc gggatttcgg gtaacgaagt tggcggtggt cggccagcag
     3361 ggggtgcagc tgattcagaa gctggtgacg gtggcgacgt tgtggattgg cgcacggctg
     3421 gtaattgagg gcaagctgac ggtggggcag ctgattgcgt ttaatatgct ctcgggacag
     3481 gtggcggcgc ctgttatccg tttggcgcag ttgtggcagg atttccagca ggtggggatt
     3541 tcggtggcgc gtttggggga tattctgaat gcgccgaccg agaatgcgtc ttcgcatttg
     3601 gctttgcccg atatccgggg ggagattacg ttcgaacatg tcgatttccg ctataaggcg
     3661 gacggcaggc tgattttgca ggatttgaac ctgcggattc gggcggggga agtgctgggg
     3721 attgtgggac gttcggggtc gggcaaatcc acactcacca aattggtgca gcgtctgtat
     3781 gtaccggagc agggacgggt gttggtggac ggcaacgatt tggctttggc cgctcctgcc
     3841 tggctgcggc ggcaggtcgg cgtggtcttg caggagaatg tgctgctcaa ccgcagcata
     3901 cgcgacaata tcgcgctgac ggatacgggt atgccgctgg aacgcattat cgaagcagcc
     3961 aaactggcgg gcgcacacga gtttattatg gagctgccgg aaggctacgg caccgtggtg
     4021 ggcgaacaag gggccggctt gtcgggcgga cagcggcagc gtattgcgat tgcccgcgcg
     4081 ttaatcacca atccgcgcat tctgattttt gacgaagcca ccagcgcgct ggattatgaa
     4141 agtgaacgag cgattatgca gaacatgcag gccatttgcg ccaaccggac ggtgctgatt
     4201 atcgcccacc gtctgtccac tgttaaaacg gcacaccgga tcattgccat ggataaaggc
     4261 aggattgtgg aagcgggaac acagcaggaa ttgctggcga agccgaacgg atattaccgc
     4321 tatctgtatg atttacagaa cgggtagaag gcgggggatg ggcgtgtagg ctgctttttt
     4381 cgtaacgttc tgcgttttca gaccgtcgtt tactgggttt actgactgaa atgaaaccaa
     4441 agtgcaggct gccggaaaga ctttttctgt ctttttaggc agcctgcact tctttttcac
     4501 tcatctagag acctttgcaa aa
//
LOCUS       DAWFIE010000112         4433 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWFIE010000112 DAWFIE010000000
VERSION     DAWFIE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.113"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            53..475
                     /locus_tag="WGQ71_001913"
     CDS             53..475
                     /locus_tag="WGQ71_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707869.1"
                     /translation="MSAVFIAPYLTAFHEQEKTFEYAELTVTAPNRSGRAIKLDADGQ
                     HYRLSCYGFDSLCTGGNIGRTIRVEQVRAVLSETVGKGFLNGVLLEYRNSGSVYSNKD
                     FARTEDRLVEVLAQPAVFCLKLGILLLLPAIFLRLKRM"
     gene            581..1144
                     /gene="pgsA"
                     /locus_tag="WGQ71_001914"
     CDS             581..1144
                     /gene="pgsA"
                     /locus_tag="WGQ71_001914"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ0707870.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1224..1299
                     /locus_tag="WGQ71_001915"
     tRNA            1224..1299
                     /locus_tag="WGQ71_001915"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1257..1259,aa:Gly,seq:gcc)
     gene            1325..1400
                     /locus_tag="WGQ71_001916"
     tRNA            1325..1400
                     /locus_tag="WGQ71_001916"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1358..1360,aa:Gly,seq:gcc)
     gene            1424..1499
                     /locus_tag="WGQ71_001917"
     tRNA            1424..1499
                     /locus_tag="WGQ71_001917"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1457..1459,aa:Gly,seq:gcc)
     gene            1521..1596
                     /locus_tag="WGQ71_001918"
     tRNA            1521..1596
                     /locus_tag="WGQ71_001918"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1554..1556,aa:Gly,seq:gcc)
     gene            1622..1695
                     /locus_tag="WGQ71_001919"
     tRNA            1622..1695
                     /locus_tag="WGQ71_001919"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1654..1656,aa:Cys,seq:gca)
     gene            1796..1885
                     /locus_tag="WGQ71_001920"
     tRNA            1796..1885
                     /locus_tag="WGQ71_001920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1830..1832,aa:Leu,seq:taa)
     gene            1989..3041
                     /gene="sohB"
                     /locus_tag="WGQ71_001921"
     CDS             1989..3041
                     /gene="sohB"
                     /locus_tag="WGQ71_001921"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ0707871.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIASAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3092..3961)
                     /locus_tag="WGQ71_001922"
     CDS             complement(3092..3961)
                     /locus_tag="WGQ71_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ0707872.1"
                     /translation="MWKMLKHIAQTHRKRLLGTFSLVGLENLWMLVLYPVFGGRAINA
                     VIAGEVWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSA
                     VTARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLL
                     PRFAAISENLYFRLKNSLERDNHFIRKGDRRQLYRHYGLLARLRVLISNREAFGYLCV
                     GTAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQ
                     RIGGLERNIKAGT"
     gene            complement(3971..4402)
                     /locus_tag="WGQ71_001923"
     CDS             complement(3971..4402)
                     /locus_tag="WGQ71_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0707873.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
BASE COUNT         1090 a         1056 c         1162 g         1125 t
ORIGIN      
        1 taatccacta tattatcgct gggtatggtt tgccgccata agctgtctgc tgttgtccgc
       61 cgtttttatt gcaccttacc tgacggcatt tcacgaacaa gaaaaaacat tcgaatatgc
      121 ggaattgacc gttaccgccc ccaaccgcag cggacgggca atcaaactgg atgcagacgg
      181 gcagcattac cggctttcct gctatgggtt cgacagtttg tgcacaggcg gcaatatcgg
      241 cagaaccatc agggtggagc aggttagagc cgttttaagc gaaaccgtcg gcaaaggttt
      301 tttaaacggc gttctgttgg aataccgcaa cagtggcagt gtctatagca ataaagattt
      361 tgcccgcacg gaagaccgcc ttgtcgaagt gttggcacaa cctgccgttt tctgcctgaa
      421 actgggtatt ttgcttttat tgcccgccat tttcctgcgt ttgaaaagaa tgtgaaaaca
      481 gattggcggg gcgggggaat gcccatgcag caattttacg ttttgggtta tgaggataac
      541 cgttataatc acaactttac agtctgcaca gagaaaaccg atgccttgga atctccccat
      601 cttcctcaca tggttgaggg tcttgctcat tcctgtcatg acagcccttt tttacctgcc
      661 ttttccgtgg ttcgcggagg aaacgattaa tcttgccgcc gccgtcattt ttgccgttgc
      721 cgccttaacc gactggtttg acggattttt ggcaagactg tggaaacaga cttccgattt
      781 cggcgccttc ctcgatcccg ttgccgacaa gctgatggtc gccgtctctt tgcttttact
      841 ggtcaaactc gaccggacct atgttttgtt tgccatgatc atcatcggca gggaaattac
      901 catttccgca ttgcgcgaat ggatggcgca aatgggcaaa aggaacagcg ttgccgtcgc
      961 caccgtcggt aagtttaaaa ccgccgcgca aatgctggca attttcctgc tgctgctgaa
     1021 tattcccgat ttttacggat ttaatcttgt tgttatcggc aatgtattga tgtttatcgc
     1081 atctttgctg acagtctggt cgatgctgta ttatctgaaa atggcgtgga aagaaatcgc
     1141 ctgaaaaaaa cataaaaata gcttgacggc aaaaacagaa tccataataa ttgcgtctct
     1201 tcgatgttgg agaatgaaat cgggcgggaa tagctcagtt ggtagagcgc aaccttgcca
     1261 aggttgaggt cgcgagttcg agactcgttt cccgctccaa aaatttcatt ttcccacaat
     1321 cattgcggga atagctcagt tggtagagcg caaccttgcc aaggttgagg tcgcgagttc
     1381 gagactcgtt tcccgctcca agttttattt caaacatatc aacgcgggaa tagctcagtt
     1441 ggtagagcgc aaccttgcca aggttgaggt cgcgagttcg agactcgttt cccgctccaa
     1501 aaaccgatat gtttgaacgc gcgggaatag ctcagttggt agagcgcaac cttgccaagg
     1561 ttgaggtcgc gagttcgaga ctcgtttccc gctccaagtt ttttagataa gccagtttta
     1621 gggcgagata gcaaagtggt tatgcagcgg attgcaaatc cgtctacgcc ggttcgattc
     1681 cgactctcgc ctccatatat attacggcgg ggtggcagag tgtttatgct atgaggagtg
     1741 caatcttcat ataggccggt taaaatccgc gcccccgcct ccacctttca caaatgcccg
     1801 ggtggtgaaa taggtagaca caacggactt aaaatccgtc gggactaaac atcccgtgcc
     1861 ggttcgattc cggctccggg caccaagctg aaaatgacaa tgccgctcca agcggttatt
     1921 tttttatctg tcggacggtg atggacaata ttgacatgtt catgcctgaa caagaggaaa
     1981 tccaatcaat gtggaaagaa attttactga attacggtat tttcctgctc gaactgctta
     2041 ccgtgttcgg cgcaattgcg ctgattgtgt tggctatcgt acagagtaag aaacagtcgg
     2101 aaagcggcag tgtcgtactg acggattttt cggaaaatta taaaaaacag cggcaatcgt
     2161 ttgaagcatt ctttttaagc ggggaagagg caaaacatca ggaaaaagag gaaaagaaaa
     2221 aggaaaaggc ggaagccaaa gcagagaaaa agcgtttgaa ggagggtggg gagaaatctg
     2281 ccgaaacgca aaaatcccgc ctttttgtgt tggattttga cggcgatttg tatgcacacg
     2341 ccgtagaatc cttgcgtcat gagattacgg cggtgctttt gattgccaag cctgaagatg
     2401 aggttctgct cagattggaa agtccgggcg gcgtggttca cggttacggt ttggcggctt
     2461 cgcagcttag gcgtttgcgc gaacgcaata ttccgctgac cgtcgccgtc gataaggtgg
     2521 cggcgagcgg cggttatatg atggcgtgtg tggcggataa aattgcttcc gctccgtttg
     2581 cgattgtcgg ttcggtgggt gttgtagcgg aagtaccgaa tatccaccgc ctgttgaaaa
     2641 aacatgatat tgatgtggat gtgatgacgg cgggcgaatt taagcgcacg gttactttta
     2701 tgggtgaaaa tacggaaaag ggcaaacaga aattccgaca ggaactggag gaaacgcatc
     2761 agttgttcaa gcagtttgtc agcgagaacc gccctcaatt ggatattgag gaagtggcaa
     2821 cgggcgagca ttggttcggt cggcaggcgt tggcgttgaa cttgattgac gagatttcga
     2881 ccagtgatga tttgttgttg aaagcgtttg aaaacaaaca ggttatcgaa gtgaaatatc
     2941 aggagaagca aagcctgatc cagcgcattg gtttgcaggc ggaagcttct gttgaaaagt
     3001 tgtttgccaa acttgtcaac cggcgggcgg atgtgatgta gtttgccgaa cggatggaaa
     3061 tgccgtctga agcgtgttca gacggcattt ttcaagttcc ggctttgatg ttccgttcca
     3121 atccccctat ccgttgtccg atgtctttca aattggaata ttgttcgacc aatcgcggca
     3181 cgtcgtccaa actcatggca aacatccaca gataagtgcc gaccgaatag acatgccccg
     3241 cgctgctgta gcctttgagc gtcatcatca caaaagcaaa gccgaacaaa atacccatcg
     3301 ccgtgccgac gcagagatag ccgaaggctt cgcggttgga aatcagcaca cgcaggcgcg
     3361 caagcagtcc gtaatggcgg tacagctgcc gccggtcgcc ttttcggata aagtggttgt
     3421 cgcgttccaa gctgttcttc aggcggaaat acaggttttc gctgatggcg gcaaaacgtg
     3481 gcaaaagcca taaaaacaac gcaagtatgc ccaccgccga cacgccgacc caaaattcca
     3541 gcaccagcag catgatgcac gcgccgaata tggatacgac ggatgtcgcg gcaatcggca
     3601 ggtgttcttc aaaaaagctg acaaactcac gcgacagggc aacccgcgca gttaccgccg
     3661 aatgcgggac ttgtcgctgc cgctgttcca acacgaccgg cacggcgatt tcggtataaa
     3721 tccgcgtaaa cgtgcgcgta tcggcaatcc gccgcgccgc accgaccagc cacatcaaaa
     3781 gcacaaccaa agcgtacagc aacgcctgcc acacctcccc cgcaatcacg gcattgatcg
     3841 cccggccgcc aaacactgga tataatacca gcatccaaag gttttccagt ccgaccaggg
     3901 aaaacgtgcc gagcagacgc ttgcggtggg tttgggctat gtgtttcaac attttccaca
     3961 tatttgctcc ctatttttta ttttccgaga ttgttttttc catcacttca gccagtgcat
     4021 ccaaatcggc aaacagccgg gttgtgcgct tgtcgccgaa tgtggcaaat actttgtcgc
     4081 tgaattcctg cgcgctttct gttaaaggtg cggcatgggc tttgcctttt tcggtcagcg
     4141 acagcaaccg ttcgcgccgg tcctgttcgc tttcctgcca ttcaatcaac ccttgtccgg
     4201 caagggtttt gcatacgcct gaaacggtct gtttgggcaa actccactct tcgccgatat
     4261 gcttttgcgt gcggctgcct tcggttgcca gggtataaag tacggcaaag aggttgtaat
     4321 tcagatcctg ctgcccgatc catttgtcga agacattgca aatcaggttg atacgggtgc
     4381 caagttggtc gagttggttc ataattggtc ttgatattga ctaaatttgt gcg
//
LOCUS       DAWFIE010000113         4432 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWFIE010000113 DAWFIE010000000
VERSION     DAWFIE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.114"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(46..350)
                     /locus_tag="WGQ71_001924"
                     /pseudo
     CDS             complement(46..350)
                     /locus_tag="WGQ71_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(478..1728)
                     /locus_tag="WGQ71_001925"
     CDS             complement(478..1728)
                     /locus_tag="WGQ71_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ0707874.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     DAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADVRVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            complement(1794..2006)
                     /locus_tag="WGQ71_001926"
     CDS             complement(1794..2006)
                     /locus_tag="WGQ71_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707875.1"
                     /translation="MADKDVVGAGYLIDFAQTAENLPFNVLLLISLVLNKGDETLKTS
                     MLNKLPMLNKLPDNAKENLRIMGYLP"
     gene            complement(2227..4002)
                     /gene="ggt"
                     /locus_tag="WGQ71_001927"
     CDS             complement(2227..4002)
                     /gene="ggt"
                     /locus_tag="WGQ71_001927"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ0707876.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGNYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(4170..4432)
                     /locus_tag="WGQ71_001928"
                     /pseudo
     CDS             complement(4170..4432)
                     /locus_tag="WGQ71_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin, Grx4 family"
BASE COUNT         1110 a         1027 c         1072 g         1223 t
ORIGIN      
        1 agctaaggcg aggcaacgcc gtactggttt ttgttaatcc actatactgt aatcagggat
       61 gctcagttcg tcaaagcggc aaaacaggtt gaagtcgatg cgggtgatga ggctgtgttc
      121 gagttcggga tcggagaggc tgtgccattg tccgagcagg acggctttga acatggacaa
      181 caggggatag gcgggacggc cgcggtaatc tcggaggtaa cgggtttttg acggttcagg
      241 tattgttcga tcggctgcca atcaatcacc tggtccaact tcaatagtgg gaaacggtcg
      301 atgtgtttgg caatcatggc ttgggcggtt tgctggaaga aggtgctcat ggaaaatccc
      361 ctaaatggct tggtgggaat ttaggggatt tggggggatt ttgcaaaggt ttccgcctga
      421 aacattatga gatttcaggc ggcattggat tgcttggcgg aatattttta aaaaggctta
      481 cgcgccgtaa acggggtatt tattgcacaa agcagttact tgttcgcgga ctttggcgag
      541 gttggcttcg tcttcggggt tagacaatac gtcggcaacc aagttcgcca atacgcgcac
      601 gtcggcttcg ttaaaaccgc gtgtggtcat ggcggcggaa ccgatgcgga tgccggaggt
      661 aacgaagggt ttttccggat cattcgggat ggcgtttttg ttgacagtga tgtgcgcttt
      721 gcccaaagca gcttcggcgg ctttgccggt aattttcatc ggttgcaggt caacgaggaa
      781 aacgtggctt tcggtgcggc cggaaacgat gcgcaaaccg cgtttaacca actcttccgc
      841 catggcggct gcattgattt tcacttgttt tgcgtattgt ttgaactcgg gttgcaatgc
      901 ttctttaaac gccacggctt tggcggcgat aacgtgcatc agcgggccgc cttgcaggct
      961 tgggaagatg gaagagttca gtgctttttc gtgtgtgttg tcgcggcaca aaattacgcc
     1021 gccgcgagga ccgcgcaggg ttttgtgggt ggtggtggta acgaagtcgc agaacggcac
     1081 gggattggga tattcgccgc cggcaaccag accggcatag tgcgccatat cgacaaagag
     1141 gtatgcgccg actttatcgg cgatttcgcg gaattttgcc cagtcgattt gcaacgcgta
     1201 ggcagacgca cccgccacaa tcattttggg tttgtgttcg agcgcgaggc gttcgacttc
     1261 ggcataatcg agcacttcgt tttcatccaa accataagta acggcattgt agagtttgcc
     1321 tgagatatta acgctcgcgc cgtgggtcag gtggccgccg tgcgccagag acatacccaa
     1381 aatggtgtcg cctggtttta aaacggaagc gtacacggct tggttggctt gcgagccgga
     1441 gtgcggttgg acgttggcgt attgcgcgcc gaacagtttt ttgacgcggt caatcgccaa
     1501 ttgttcgaca atatcgacgt attcgcagcc gccgtagtag cgtttgccag gatagccttc
     1561 ggcgtatttg ttggtcaatt gcgaaccttg cgcgtccatt acggcgcagc taacgtagtt
     1621 ttcggaagca atcagctcga cgtggtcttg ctggcgttgg tcttcttggg caatggctgc
     1681 tgccaaatcg gggtcgtatt gtgcgagggt aacgcttttt gaaaacatgt tctcggctcc
     1741 tttgtgtaat cagggtatca tgagtgtttt ttgtataaaa aatacttcaa tacctaaggt
     1801 agatagccca taatgcgtaa attttctttg gcattatcag gtaatttatt taacatgggt
     1861 aatttattta acatgctggt ttttagcgtc tcatcacctt tatttagtac aagactaatc
     1921 aaaagcaata cattaaatgg taaattttcg gcagtttgtg caaaatcaat caaataacca
     1981 gcaccaacca catctttatc agccataacc cgctgataca ccacttgcta agacaactct
     2041 cctaccccac catgtcttaa aatcagatga atggcttgta atgctgtgtt tggtgtctca
     2101 caaattgcca aaatctgatc agaagcttgc ttgatggctt gtatttttgt ttgattactc
     2161 acaatttccc cctattttaa taattaactt aaatgcggtc aaattcacaa aatacaagct
     2221 tcacctctaa tcacccatca ctcgaccttc tcggcgtgga tcggcaccac caaccagcct
     2281 gcttggctcg ataataatgg cttgaacacc tgaatttagc tcacgcacat cagtcttata
     2341 gcccaaatca tttaatgctt gttgccactg gagggcggtt gtgcccgttt ctagttcata
     2401 gttaccaaag cgatttaata aattgggtgc actgatggca tcttggatat ccatattcca
     2461 gtcactatgt gccacaatcg tcttagcgac atagccaatg atacggctac cacctgggga
     2521 gccgattgcc atataaggct tgcctgcttt aaatacgatg gttggtgcca ttgaggagcg
     2581 tggtctcttg ccgggctcga cacgattggc gacctgtttg ccctgcttta ttggctcaaa
     2641 actaaagtct gtcagctcat tattcagcag gtagccattt gccatcaaag ttgagccaaa
     2701 cgcattttca atggaagtcg tcattgatag cacattgccc gccttatcca caattgatat
     2761 atgactggta gaaggtaact caatcgcttg tgaggacacc cactcatgaa taaaatcgcc
     2821 tgcagatacg ctaggcaatg ccttatccga ctgctcaagc agctggctgc gatgttttag
     2881 gtagtcttta gaaatcaact ggcgaatggg tactggtaca aaatcagggt cgcccaaata
     2941 tacatcacga tccgcaaacg caagcctaga agcgtcgccc aagagacgta aaccttcagc
     3001 atcatacccc acctgattgg gtgaaaattc atttaaaatc cccaaaatct gacccacagc
     3061 aatcccacct gagcttggtg cacccatacc gcatacttca taaatacgat aagtcacaca
     3121 aacaggcggg cgttccacca cttgataatc agataaatct tgtaaggata attgaccggg
     3181 gttatcctta gcattttgga caactgaaac gatattttgg gcatatttac cagtatgcag
     3241 agcttttgca ccttgagctg ctaacgcctg aacactgtca gcaaattcta aatttttcag
     3301 caagctgcct gcttgtagcg gcacaccatt cggcaaaaaa taagcggctg tttttggata
     3361 gcgtgccaaa tgctgctgat tttgctcaac cgagatggca agccttggcg acacctcaaa
     3421 gccttgtttt gccaagcgga tcggcgtatc aaataatttt ccccaaggca atacaccgta
     3481 tcgctgatgt attgtctcca tcagtttagg gatagcaggc gtacccaccg agcgaccacc
     3541 gaccaccgct tccataaatt tcaatggttg accatcttta tccaaaaata attccggcgt
     3601 cgcacgcatc ggtgccgtct cacgcccatc aaatgtggtc aatgttttgg cggtattatc
     3661 ccaatacaac acaaatgctc caccgcccaa gcctgacgac tgtggctcta ccaagcttag
     3721 tgtcgtctgc accgccacca tcgcatctgc agcgctaccg ccttgcttta agatatcata
     3781 gccagcttgt gttgctaatg gattggctga cgctaccata aaatcacttg caatcacctg
     3841 cttttgttcg gtcagtcccg ttgcatgttc aggcgtgtga gcgtctgcac ctgtgatgac
     3901 agcagaatga gtattaacct taccttgatt ggcatggatg acttgacatc cagagattgt
     3961 catagacatt atcaatgcag tcaataaata tgttttagcc acaagcactc cttcgcctga
     4021 gtttgattga tgattcatac aaggcatgct gattattgta tttaatatgg ctaaataatt
     4081 caatccaaac tatcaatctt gaccatcaaa aaaagaccgc taatgtcatc agcagtcttt
     4141 tttgatattt attttaagat attaagtaat cagacctttg ggctatgctc ttcaatgagt
     4201 ggttttagct cacctgattg gtacatttgt aggataatat cactaccacc gattaactca
     4261 ccattaaccc aaagctgtgg aaaggttggc cgattggcga tgagtggtag agtactgcga
     4321 atttctgggt tttctaggat attaacaaaa gcaaagggtc tgccaattgg gtcagcacct
     4381 ctactgcacg cgctgaaaat ccacattggg gaaactgggg cgtgcctttc at
//
LOCUS       DAWFIE010000114         4298 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWFIE010000114 DAWFIE010000000
VERSION     DAWFIE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.115"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            496..2382
                     /locus_tag="WGQ71_001929"
     CDS             496..2382
                     /locus_tag="WGQ71_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ0707877.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAESWLARHSDGIPAGIST
                     PQSSVSETDYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDVEGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQTD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPNTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLNPCKSGLGFEGIFNV
                     VIRTLLLKPVSNSVSDDLDSRLNLNQDKATKPQTRQDEATPYRFKVNPLYQGVYGVGS
                     GIVIDSDPAAEYRECGWKARFLNELRPDFGIFETLRVENGRCTLLDRHLCRLKTSAQA
                     LNLPLPDGCENQIKQYIADLPDGAFRIKALLASDGISLSRAVLNRLTDKQRVIISPTI
                     LPAQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQW
                     LTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFA
                     AALA"
     gene            complement(2443..3960)
                     /locus_tag="WGQ71_001930"
     CDS             complement(2443..3960)
                     /locus_tag="WGQ71_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0707878.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALSQVWRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRKLHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAEKLDLPAPVLENVGRFRKKAEGLTGWRFSGGISPAVNRNANNAAPQYCWQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLSPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
BASE COUNT         1062 a         1379 c          958 g          899 t
ORIGIN      
        1 cgacggagcc gcagacagta caaatagtac ggaaccgatt cacttggtgc ttcagcacct
       61 tagagaatcg ttctctttga gctaaagcga ggcaacgctg tactggtttt tgttaatcca
      121 ctatacttgg agctggtctt gcttttcgcc taattctacg ttttcaaacg gttgcagctg
      181 gtggtctgcc ataaaggtct ccttattgta tttcaggttg gaaatgtttc aacacacagg
      241 acgacacata aagcgtcgcc ctatgtgttg ccctgatttg gaaggggtta cgcccctccc
      301 aaataaagtc tgattctacc gccctaaagg gcggggttta aaccgaaaag gaaatacgat
      361 gaaaatccat tttcacaatt ttacaccttc gcccccgctt tctctacata aaattacatt
      421 ttgccgatat ttgccgaatt gtctgaaaat atgtgtaaaa agagcgtata atcaaaacat
      481 ttgccccgaa ttgccatgcc ttatttcgcc ctgtttgacg atgccgtaag cggccgcgca
      541 aaacgctatc aaaatcatgt agaaagccgt tttttccgtc ccgaagaact cgatgctttg
      601 gacggcgcgc tgcaatcggg ctggcaaaaa gggctgcatt cggtgttgtt tgcagactac
      661 ggattcggtc tgccgctgac gggggttgag tccgaacgcg gcggcaatct tgccctgcac
      721 tggtttgccg actgcgccga caccgatgcc gaaagctggc ttgcccgaca ttcagacggc
      781 atccccgccg gcatttccac gccgcaatcc tccgtatccg aaaccgatta cctcgaccac
      841 atccgccaaa tccacgaagc catccggcgc ggcgacacct atcaaatcaa ctacaccacc
      901 cgcctgcacc tgcaagccta cggcaatccc gtcagcctct accgccgcct gcgccagccc
      961 gtcccctatg ccgtcttgtc ccacctgccc gatgtggagg ggcaatccgc gtggacgctg
     1021 tgtttctcgc ccgaactctt cctcaaaatc ggttcggacg gcaccatcag caccgaaccg
     1081 atgaaaggca ctgcgccgat tttgggcgac ggacaagacg aacgccgcgc cgccgagttg
     1141 caaaccgacc cgaaaaaccg cgccgaaaac gtgatgattg tcgatttgct gcgcaacgac
     1201 ctcggcaaaa tcgcccaaac cggcaaagta tgcgtgcccg agccgtttaa agtatcgcgt
     1261 ttcggcagcg tctggcagat gaccagcacc atccaagccc aagccctgcc aaacacctcg
     1321 ttcgccgaca tcctccgcgc cgccttcccc tgcggcagca tcaccggtgc gcccaaaaaa
     1381 atgagtatgc agattatcga atcgctcgaa accgaagcgc gcggcctgta caccggcagc
     1441 atcggctacc tgaatccatg taaaagtggg ctggggtttg aaggcatatt caacgtcgtt
     1501 atccgcacct tgttgctcaa acccgtttct aactcagttt cagacgacct agatagtcga
     1561 ttaaatttga atcaggacaa ggcgacgaaa ccgcagacac ggcaagacga ggcaacgccg
     1621 taccggttta aagttaatcc actctatcaa ggggtgtacg gtgtcggttc cggcatcgtc
     1681 atcgacagcg accccgccgc cgaatatcgc gaatgcggct ggaaagcccg tttcctcaac
     1741 gaattgcgcc ccgatttcgg catttttgaa accctgcgcg tcgaaaacgg acgctgcacc
     1801 ctgctcgacc gccacctatg ccgtctgaaa acctccgcgc aagccctcaa cctgcccctg
     1861 cccgacggtt gcgaaaatca aatcaaacaa tacattgccg acttgcccga cggcgcgttc
     1921 cgcatcaaag ccctgctcgc ttcagacggc atcagcctgt cccgcgccgt tttaaaccgt
     1981 ctgaccgaca aacaacgcgt catcatttcg ccgaccatcc tgcccgcaca aaactacctg
     2041 cgccgcttca aaaccacctg ccgcaccgtc ttcgaccaag cgtggcaaac cgccgaaaca
     2101 caaggcgcgt tcgacagcct gtttttcaat tcagacggca tcctgctcga aggcggcaga
     2161 agcaacgtgt tcgtcaaaca tcgcggacaa tggcttacgc cctctttaga tttggacatt
     2221 ttaaacggca taatgcgcca agccgtattg gacgaaccgc aaaaatattt gcaaacaaat
     2281 caagtaatcg aaacacacat cacacaaaaa acactgcaag aagccgaaga aatccgcctc
     2341 tccaacgccc tgcgcggcgt atttgccgcc gcccttgcct gaacgcgcaa aaatgccgtc
     2401 cgaacctgtt tccacaaggt tcagacggca ttattccacc attcaaaacc gccaatccgc
     2461 cgacacaaac acctcgctgt tgcggcgttt cgcatacggc acattacttt ccgtcctgcc
     2521 gaaacgataa ttcaacgccg gtacgatacc tttgtacgac aacttgtcgt ggctcaaagc
     2581 cagcgagaca ttccattcgc ggttgcgttg cgcctctgtc gagaaagccg caacgccctt
     2641 atagttgcgg cgggcataag acgcggaaac ccgactgttc aaaccgccca actgccgcca
     2701 ctcctgcgcc cagccggcat aaacaccgtt gcgccggtag gcggcattat tgaccgcgcc
     2761 gcccaccgtt tcgcgtttcg gcacaaaccg cacaaactgc cagccgccga acacagttgc
     2821 cgattcgccc aaacgttttg ccgacgaaac ataaaacccg tcctgccggc cgttattgta
     2881 ttccgcccta tcctgttcgc ggtagcgttg gcggtaatgt tccagcgcga ccgaaaattg
     2941 ccatcccggg tttgggcggt aagtatggga cagctgcacg ccgactccgt gcgccagcat
     3001 atacggcgac aggcggcggt tgtttacccg ttttgttttc gcatcaaagc cgtcgctgcc
     3061 cgacaactgc acctgataaa acggcaaaat ccccgccgtc tgccgcgcat ttttatactg
     3121 ccatcccaaa tacgccctgc cgaacccgtc atcataagct gattttttac tgaaataata
     3181 gctcgtgccg ccgatattgg aacggaacaa caaataatga ttgtccgcca acgccgtcag
     3241 cttttccgcc tcgatttcat aattcaaccc tgccgcccgc tccgcccggc tgacactgca
     3301 tatctgccgg cctccgtttt gccagcaata ctgcggcgcg gcattattgg catttctatt
     3361 gaccgccggg ctgataccgc ccgaaaaacg ccagcccgtc agcccctccg cttttttccg
     3421 aaaacgcccc acattttcca aaaccggcgc cggcaaatcc aatttttccg cctcggcaaa
     3481 atgcctttct gccgacttca gccggaaatc gtcaaactcc gccgccgcca aatccagcaa
     3541 aatccgctcg tctgccgcat tttccccgtg cagcttccga taccgcgcca ccgcctccgc
     3601 tggccttccc gccaatttcg ccagcaaagc ccgcgccctg ccgtacaaaa ctgcatcata
     3661 atccggcagc ttggcataca aatccgccaa cgaagcgatt aaatccgcct gattgccgtt
     3721 gagcgcgtcg cgcaaactat gttccaacat tttcgggtgc gccaacaaaa aatccccgtc
     3781 aaccacgcgc ggggcatcat tttcaacttt ccaatccgat tccgcccatt tatccgacac
     3841 cgaccgccac acctgcgaca aagctttgtc atccaaaatt gcaggcgcat ccgccccata
     3901 ggcggcagaa acacctgccg cacaccaaac aaccaaaaaa ccgtatctga aatacaacat
     3961 accctgtcat ttacctttcc ggcaaacacg ccgccgaagc acgtcaaacc atccgaaaaa
     4021 caggcaaaag cccgtaaaaa ccggctttgc cgcctgaaag cgggcaggca aaaaaccgcc
     4081 gcccgatttt caaagggcgg atttcacatt tatagtggat taacaaaaat caggacaaga
     4141 cgacgaagcc gcagacagta cagatagtac ggaaccgatt cacttggtgc ttcagcacct
     4201 tagagaattg ttctctttga gctaaggcga ggcaacgccg taccggtttt tgttaatcca
     4261 ctataacagc aaccctgtcg ccgtcattcc cgcaaaag
//
LOCUS       DAWFIE010000115         4275 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWFIE010000115 DAWFIE010000000
VERSION     DAWFIE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.116"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(169..384)
                     /locus_tag="WGQ71_001931"
     CDS             complement(169..384)
                     /locus_tag="WGQ71_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707879.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLEMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(394..636)
                     /locus_tag="WGQ71_001932"
     CDS             complement(394..636)
                     /locus_tag="WGQ71_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0707880.1"
                     /translation="MDRLKAAYLSARPDMVLLLRYTEMGLNQAEAAKLLGIAPSNVRA
                     RLKQLADLGLTEYRPDPKYRNRHALAAAHGQQSLGL"
     gene            complement(894..1079)
                     /locus_tag="WGQ71_001933"
     CDS             complement(894..1079)
                     /locus_tag="WGQ71_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707881.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            complement(1352..1519)
                     /locus_tag="WGQ71_001934"
     CDS             complement(1352..1519)
                     /locus_tag="WGQ71_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707882.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            2042..2233
                     /locus_tag="WGQ71_001935"
     CDS             2042..2233
                     /locus_tag="WGQ71_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707883.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            2230..2646
                     /locus_tag="WGQ71_001936"
     CDS             2230..2646
                     /locus_tag="WGQ71_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707884.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDSD"
     gene            2823..3227
                     /locus_tag="WGQ71_001937"
     CDS             2823..3227
                     /locus_tag="WGQ71_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HEZ0707885.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCTECSIPEL
                     ERVVEDLRQHGFTPKKTAGRQPNRRSSADPMMRKIEALLLDNGWSWNYAQGTAKKMFK
                     VDRVEWLSDGNMHKLVAALQISANRKKKEKTG"
     gene            3224..3703
                     /locus_tag="WGQ71_001938"
     CDS             3224..3703
                     /locus_tag="WGQ71_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707886.1"
                     /translation="MSLNWEMTEQDFEAVKHLLPHSVVAMITVIGLEAAFHMVKVWGG
                     TNYPISNRRRNTRQSRILHAQLVEDIGEEAAGRLERAYVGQPFLAIPRCWDAMRELRN
                     RFIRRQYDAMSAEGLSDLFIVRELVLAHRLSARNIRYILKEADREAAARAQTDLFVA"
     gene            3866..>4275
                     /locus_tag="WGQ71_001939"
     CDS             3866..>4275
                     /locus_tag="WGQ71_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0707887.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNSAAGKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGRHSRLAYA"
BASE COUNT         1050 a          917 c         1130 g         1178 t
ORIGIN      
        1 tgggtcagtt gcgtgtagct catggcaatc tttcttgcag gaaaggtcgt atgctaccgc
       61 atactggcct ttttctgtta gggaaagttg cacttcaaat gcgaatccgc cgtcgtctga
      121 aacggggttt aaaacctgtt tcagacggcc ttttttcatg ccctagattc aggctgcttt
      181 ctcttcctgc tcgtacacgg cgttgtagaa agcggcggac agcttgtcgg ctttctcgga
      241 ggcggtttca atcacggcag acaaaccgtc ttcgattcct tccatgtcca tctccaaaac
      301 tttcagatgg ttgagtgtga acaccagcag attcagggct ttgaggctgt cttggtcgaa
      361 agtcagggta taggtggtat tcatggctta accctacaat cccaaagact gttggccgtg
      421 tgccgccgcc agggcgtggc ggttgcggta tttcgggtcg gggcggtatt cggtcagacc
      481 cagatcggcg agttgtttga ggcgcgcgcg cacgttggac ggcgcaatgc ccaagagttt
      541 ggcggcttcc gcctgattca gtcccatttc ggtgtagcgc agcaggagca ccatatcggg
      601 gcgggcggaa aggtaggcgg ctttgagcct gtccattgcg ctctgtaaac tctttagagc
      661 attccaaatt ttgaatggcg cgtaacacat ctttgtgctt cttgccgaaa ttgtttgata
      721 cggcgagcga ggttgtaacc ggacggtcgc cggagatttg aacaagttgg gtgttgttgg
      781 tattcatgat agaatagcct ctcttttaga ttgcatttaa aagacacgag aaagaacgtt
      841 gcagcgttgc tttcccactg ttcccccgat gttccaccat cgggggaatt ttcttatttc
      901 attttttcct tcatgactga tttaacccag tttgaaaagt cgggaatttc tttttgcata
      961 aaatccacca actctttctc tctttccaaa tggaaagaaa ctgttttcat cagccgcttt
     1021 tggtcatact ttcgctgata ttcagcttgt tttgctttta gcgttttttc atcaggcata
     1081 ggcaatcctt gatttttaaa atcgttgtag taaaatggct tgcagaaagg ttggcgaaag
     1141 gggcgcggtg ttctgccacc ccttgtgttt aatcaacttg cttagtaagc cggactacag
     1201 cttaaaagca gaatgatgaa aaaaacaact ttcgccagtt ttgtcatttc cacaagcctc
     1261 ctttctatcc gataagcccc cgaagttgcc gcttcggggc tttgctttat cagataggcg
     1321 tattatctag tatttctaga atttagtcaa gctatttttt gattttttct ttgatgattg
     1381 atttaaccca ttgggaaaaa tcgatatttt gggcatattc caaaagttct ttttccgttt
     1441 cggcattgaa cgagactttc ttaatcaccc gtttctgctc gtaacgcttt cggttttctg
     1501 ccagtttttc atcaaccata ggcaaactcc ttgatttttt aaaccgtatt ttgtaagatt
     1561 gggactgggg acagcggcga ccgcccccag ctggtcagtc tttagtaggc attagcactt
     1621 gccaacatga aaactgcgac caagatgaga atttgaatag tgcgtttatt catcttcttt
     1681 ctcccagttg agccccgctt cggtggggct tttcccgtat cggccctcac tacgccgatg
     1741 aattgaatta taaacgtgga cacgttaaaa gtcaaccctt tccccaacaa aatcaaagaa
     1801 aaatgccgtc tgaaaacagc tcgaatgctg tttcagacgg cattttttgt tgtcgggcat
     1861 caatgccctg ccatttgtgc ggcgttgcgg ttatcaagat atggtatttt agtgtgtatt
     1921 tttagaagaa atttaccata tatagtatta aaatattata cggttgtttt atatggattt
     1981 gattttcttg aaaaataaat cccatatgct aatatttgca ctatttgggc gaggttgaaa
     2041 aatgaatacc gtcaaagaat atacggcagt ccgcgaacgg ctgctcaatg ctgccgatta
     2101 tttggaagaa gtccgtaaag accgtaagac gggcaatatt gcgtccgtgg aatttgtgcc
     2161 gccgaaaata ggggctcggg gttacggcaa attcaaagtc cgttacaaaa ccttagtggc
     2221 ggttgattta tgagcgggga aaaatccgag aatctgccgg ccgatgagca gcagaaaatg
     2281 cttcaggagt ttttagaact tcaaaaacag gatattgcag tcaaacagaa ggagttggaa
     2341 agccgtaagg acgaaatcca atctcacgaa cgcattgcac tggctaccat cgaagcgcaa
     2401 aaacagggtg aacagcaaca tggtgagatt ttcaaagagg tacacaaacg ccgtctgaat
     2461 gccgtgattg ctatttcttt tctgattgcc gccgtgctga ttactgcttt atggcgcgat
     2521 aaggacagcg tcgctattga aattgtcaaa atcggcgggg cggtagcttt gggatatttt
     2581 gcaggaatca acagaggtaa agcgcaaacc ttggaaaagc aacggcgcga aaaagacagt
     2641 gattagattg tagtaaaaaa ggccgtctga aatgttttgg cgaaactcgc aaagctcgtt
     2701 ttcagacggc tttttcatgc ctgtccgttt cgtaaaaaaa acagcggctt actacaatat
     2761 atagtatttt atatgtataa tatgcatcaa ttaatcaata tattgtgttt tatgggtttg
     2821 aaatgcgccg ggcgttgatt gcgaaaatta agattgctca aaaggagctg ggcttggatg
     2881 acggtaccta tcgagcggtg ttagagcggg tgacgggcaa gcggtcgtgt acggagtgca
     2941 gtatccctga gctggaacgc gtggtcgagg atttgcgcca acatggtttt acgccgaaaa
     3001 aaacggcagg ccgacagcca aaccgccgct cttctgccga cccgatgatg cgcaaaatcg
     3061 aagccctgct gctggataac ggctggtctt ggaattatgc gcagggtacg gcgaaaaaga
     3121 tgtttaaggt tgaccgcgtg gaatggttgt ccgacggcaa tatgcacaag ttggtggcgg
     3181 ctttgcagat tagcgcgaac cgcaagaaaa aggagaaaac gggatgagtt tgaactggga
     3241 gatgacagag caggactttg aggctgtgaa acatctgctg ccgcacagtg tggtggcgat
     3301 gattacggtt atcgggctgg aggcggcgtt tcacatggtt aaggtttggg gcgggacgaa
     3361 ttatccgatt tccaaccgcc gccgcaatac gcgtcagagc cgcatcttac acgctcaact
     3421 ggtcgaggac atcggcgaag aagcagcggg acggttggag cgtgcttatg tcgggcagcc
     3481 tttcttggcg attccgcgct gctgggatgc gatgcgcgaa cttcgcaacc ggttcatccg
     3541 ccgccaatat gatgcgatga gcgcggaagg tttgagcgat ttgtttattg tgcgcgagct
     3601 ggtattggct catcggctgt ctgcgcgcaa tatccgatac attttgaaag aggccgaccg
     3661 cgaggcggcg gcaagagcgc agacggattt atttgtagct tgattgtgtt gtgtgtttct
     3721 tgtgagtgga cctttgccct gctttttgca gggctttttt ttgtctgcgg aacagtaagt
     3781 gcatttgtgc cgactgtctt atgccgtctg aaaaggttaa ataaggtttt gaaaataaat
     3841 tgtgatttga ttttcggaga tgtttatggg caaaactgta actttaaccg ccggccacag
     3901 caacaccgac ccgggtgcgg tcaacggcag cgaccgtgag gcggacttgg cgcaggatat
     3961 gcgcaacatc gtggctgcta ttttgcgcga tgactacggt ttgactgtta aaaccgacgg
     4021 cacaggcaaa ggcaatatgc cgctgcgtga agctgtaaaa ctgattcgcg gctcggatgt
     4081 ggcgattgag ttccatacca actcggcggc cggtaaagca gctacgggca ttgaggcgtt
     4141 gagtaccgtc aaaaacaaac gctggtgtca ggtgttgagc aaagccgttg ccaagaaaac
     4201 cggctggaaa ctgcgcggcg aagacggctt taaacccgac aatgcgggcc ggcattcgcg
     4261 cctggcttat gcgca
//
LOCUS       DAWFIE010000116         3910 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWFIE010000116 DAWFIE010000000
VERSION     DAWFIE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.117"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            390..722
                     /locus_tag="WGQ71_001940"
     CDS             390..722
                     /locus_tag="WGQ71_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0707888.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGSTSAPDFNAG
                     GTGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPESA
                     YRRLYKPK"
     gene            736..3597
                     /locus_tag="WGQ71_001941"
     CDS             736..3597
                     /locus_tag="WGQ71_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEZ0707889.1"
                     /translation="MINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGY
                     NENYKNYTAYMRKEAPEDGGGKDIKASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHI
                     IGGRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFG
                     TTSRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNML
                     FIFSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLS
                     APYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIG
                     AVGVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKG
                     GSQLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIV
                     YLADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAG
                     YLNRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRG
                     NAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMP
                     GIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLD
                     HNGTGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGRSTW
                     SENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGT
                     LMQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGL
                     AGLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVA
                     GLGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1057 a         1061 c         1035 g          757 t
ORIGIN      
        1 caacgaagcc gaagacagta cagatagtac ggaaccgatt cactcggtgc ttcagcacct
       61 tagagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttcatcca
      121 ctataactag ggaaattcaa attaagttag aattatccct atgagaaaaa gccgtctaag
      181 ccggtataaa caaaataaac tcattgagct atttgtcgaa agttcaaatt tccattttaa
      241 aacaattagt aaaatcgagt ttatcctagt tgtccaagac aacccctata ataatataat
      301 tcaaaatata aaaatgggtt acatctaaac attacggaat ttttattccc tcgcctgaat
      361 tctattgtca gattcaagga gacctcatca tgcgaacgac cccaaccttc cctacaaaaa
      421 ctttcaaacc ggctgccatg gcgttagctg ttgcaacaac actttctgcc tgcttaggcg
      481 gcggcggagg cagcacttct gcgcccgact tcaatgcagg cggcaccggt atcggcagca
      541 acagcagggc aacaacagcg aaatcagcag cagtatctta cgccggtatc aagaacgaaa
      601 tgtgcaaaga cagaagcatg ctctgtgccg gtcgggatga cgttgcggtt acagacaggg
      661 atgccaaaat caatgccccc ccccccgaat ctgcataccg gagactttac aaacccaaat
      721 gacgcataca agaatttgat caacctcaaa cctgcaattg aagcaggcta tacaggacgc
      781 ggggtagagg taggtatcgt cgacacaggc gaatccgtcg gcagcatatc ctttcccgaa
      841 ctgtatggca gaaaagaaca cggctataac gaaaattaca aaaactatac ggcgtatatg
      901 cggaaggaag cgcctgaaga cggaggcggt aaagacatta aagcttcttt cgacgatgag
      961 gccgttatag agactgaagc aaagccgacg gatatccgcc acgtaaaaga aatcggacac
     1021 atcgatgtgg tctcccatat tattggcggg cgttccgtgg acggcagacc tgcaggcggt
     1081 attgcgcccg atgcgacgct acacataatg aatacgcatg atggaaccaa gaacgaaata
     1141 atgtctgcag ccatccgcaa tgcatgggtc aagctgggcg aacgtggcgt gcgcatcgtc
     1201 aataacagtt ttggaacaac atcgagggca ggcactgccg accatttcca aatagccaat
     1261 tcggaggagc agtaccgcca agcgttgctc gcctattccg gcggtgataa aacagacgag
     1321 ggtatccgcc tgatgcaaca gagcgattac ggcaacttgt cctaccacat ccgtaataaa
     1381 aacatgcttt tcattttttc ggcaagcaat gacgcacaag ctcagcccaa cacactgacc
     1441 ctattgccat tttatgaaaa agatgctcaa aaaggcatta tcacagtcgc aggcgtagac
     1501 cgcagtggag aaaagttcaa tggctccaac cattgcggaa ttactgccat gtggtgccta
     1561 tcggcaccct atgaagcaag cgtccgtttc acccgtacaa acccgattca aattgccgga
     1621 acatcctttt ccgcacccat cgtaaccggc acggcggctc tgctgctgca gaaatacccg
     1681 tggatgagca acgacaacct gcgtaccacg ctgctgacaa cggctcagga catcggtgca
     1741 gtcggcgtgg acagcaagtt cggctgggga ctgctggatg cgggtaaggc catgaacgga
     1801 cccgcgtcct ttccgttcgg cgactttacc gccgatacga aaggtacatc cgatattgcc
     1861 tactccttcc gtaacgacat ttcaggcacg ggcggcctga tcaaaaaagg cggcagccaa
     1921 ctgcaactgc acggcaacaa cacctatacg ggcaaaacca ttatcgaagg cggttcgctg
     1981 gtgttgtacg gcaacaacaa atcggatatg cgcgtcgaaa ccaaaggtgc gctgatttat
     2041 aacggggcgg catccggcgg tagcctgaac agcgacggca ttgtctatct ggcagatacc
     2101 gaccgatccg gcgcaaacga aaccgtgcac atcaaaggcg atctgcagct gggcggcgaa
     2161 ggtacgctgt acacacgttt gggcaaactg ctgaaagtgg acggtacggc gatgaccggc
     2221 ggcaagctgt acatgtcggc acgcggcaaa ggggcaggct atctcaaccg taccggacaa
     2281 cgtgttccct tcctgagtgc cgccaaaatc gggcgggatt attctttctt cacaaacatc
     2341 gaaaccgacg gtggtctgct ggcttccctc gacagcgtcg aaaaaacagc gggcagtgaa
     2401 ggcgacacgc tgtcctatta tgtccgtcgc ggcaatgcgg cacggactgc ttcggcagcg
     2461 gcacattccg cgcccgccgg tctgaaacac gccgtagaac agggcggcag caatctggaa
     2521 aacctgatgg tcgaactgga tgcctccgaa tcatccgcaa cacccgagac ggttgaaact
     2581 gcggcagccg accgcacaga tatgccgggc atccgcccct acggcgcaac tttccgcgca
     2641 gcggcagccg tacagcatgc gaatgccgcc gacggtgtac gcatcttcaa cagtctcgcc
     2701 gctaccgtct atgccgacag taccgccgcc catgccgata tgcagggacg ccgcctgaaa
     2761 gccgtatcgg acgggttgga ccacaacggc acgggtctgc gcgtcatcgc gcaaacccaa
     2821 caggacggtg gaacgtggga acagggcggt gttgaaggca aaatgcgcgg cagtacccaa
     2881 accgtcggca ttgccgcgaa aaccggcgaa aatacgacag cagccgccac actgggcatg
     2941 ggacgcagca catggagcga aaacagtgca aatgcaaaaa ccgacagcat tagtctgttt
     3001 gcaggcatac ggcacgatgc gggcgatatc ggctatctca aaggcctgtt ctcctacgga
     3061 cgctacaaaa acagcatcag ccgcagcacc ggtgcggacg aacatgcgga aggcagcgtc
     3121 aacggcacgc tgatgcagct gggcgcactg ggcggtgtca acgttccgtt tgccgcaacg
     3181 ggagatttga cggtcgaagg cggtctgcgc tacgacctgc tcaaacagga tgcattcgcc
     3241 gaaaaaggca gtgctttggg ctggagcggc aacagcctca ctgaaggcac gctggtcgga
     3301 ctcgcgggtc tgaagctgtc gcaacccttg agcgataaag ccgtcctgtt tgcaacggcg
     3361 ggcgtggaac gcgacctgaa cggacgcgac tacacggtaa cgggcggctt taccggcgcg
     3421 actgcagcaa ccggcaagac gggggcacgc aatatgccgc acacccgtct ggttgccggc
     3481 ctgggcgcgg atgtcgaatt cggcaacggc tggaacggct tggcacgtta cagctacgcc
     3541 ggttccaaac agtacggcaa ccacagcgga cgagtcggcg taggctaccg gttctgacgg
     3601 acaggaagca gacagccgca aagatcacgg tctttgcggc tgtttcttat gaaaagaaaa
     3661 ccctattcca attgcctgct tctattgttt caagacttct tccaaagatt cggcattaat
     3721 cagatgtata gcggattaac aaaaatcagg acaaggcggc gaagccgcgg acagtacaaa
     3781 tagtacggaa ccgattcact cggtgcttca gcaccttaga gaatcgttct ctttgagcta
     3841 aggcgaggca acgctgtact ggtttttgtt aatccgctat attccaccat ctctaagatt
     3901 tacagcgata
//
LOCUS       DAWFIE010000117         3799 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWFIE010000117 DAWFIE010000000
VERSION     DAWFIE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.118"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            358..1479
                     /gene="dnaJ"
                     /locus_tag="WGQ71_001942"
     CDS             358..1479
                     /gene="dnaJ"
                     /locus_tag="WGQ71_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ0707890.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1675..3693
                     /locus_tag="WGQ71_001943"
     CDS             1675..3693
                     /locus_tag="WGQ71_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ0707891.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(3737..>3799)
                     /locus_tag="WGQ71_001944"
     CDS             complement(3737..>3799)
                     /locus_tag="WGQ71_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0707892.1"
                     /translation="KDLAGKTWAQAEKLRLTLSR"
BASE COUNT          798 a          856 c         1142 g         1003 t
ORIGIN      
        1 tgctggcgaa gctgaaggat caggcttccg tcggcgcgcc tgcaccaaaa ggcccagcca
       61 aaccagtgct gcccgcggat aaaaaataaa atagggggaa gtctgcagcc gcatcaaatg
      121 ccgtctgaac atgcgttcgg gcggcgtttt tataacaaaa acacttcatg gcggttggtt
      181 ttatgcctat ctaagttttt gtgtcgtgca tacctgaaga tttcagacgg catcggttta
      241 tgctgtctga aaagtgtatt ccgtttcagt ttgtaagcta tggcagtctg tttgtcttgt
      301 gttttgcgca attgccctta ttttaagccg tgattttatt ttgaattaga tgaaaaaatg
      361 agtaatcaag atttttatgc gacgctgggt gtggcaagaa cagctaccga tgatgagatt
      421 aaaaaagcct accggaaatt ggcaatgaaa taccatcccg accgcaatcc tgacaataaa
      481 gaggcggaag agaagtttaa agaagtacaa aaggcgtatg aaactttgtc cgacaaggaa
      541 aagcgcgcta tgtacgacca gtatggtcat gcggcgtttg aaggcggcgg acaggggggc
      601 ttcggagggt ttggcggatt tggcggtgcg cagggttttg actttgggga tattttcagc
      661 caaatgtttg gaggcggttc ggggcgcgcc cagcctgatt atcagggtga ggacgttcaa
      721 gtcggtatcg aaatcacgct tgaagaagcc gcaaaaggtg tgaagaaacg catcaatatt
      781 ccgacttatg aagcgtgtga tgtctgtaac ggcagtggcg cgaaaccggg gacatccccg
      841 gaaacctgcc cgacttgcaa aggttcgggt acggtgcaca tccagcaggc gattttccgt
      901 atgcagcaga cttgtccgac ctgccacggt gcgggcaaac acattaaaga accttgcgtc
      961 aaatgccgtg gcgcggggcg gaataaggcg gtcaagacgg tggaagtcaa tattcccgcc
     1021 ggtatcgatg acgggcagcg tatccgtttg agcggcgaag gcgggccggg tatgcacggt
     1081 gcgcctgccg gcgacttgta tgtaaccgtc cgcattcggg cgcataagat tttccaacgc
     1141 gacggtctgg acttgcattg cgaactgccg atcagttttg ccacggctgc tttgggcggg
     1201 gagttggaag tgccgacctt ggacggaaag gtcaagctca ccgtccccaa agaaacccaa
     1261 accggcagga ggatgcgcgt gaagggtaag ggtgtcaaat ctttacgcag cagcgcgacc
     1321 ggcgatttgt actgccatat tgttgtcgaa acgcctgtca atttgaccga ccgtcaaaaa
     1381 gagcttttgg aagaatttga gcggatttct accggcttgg aaaaccaaac accgcgcaag
     1441 aaatcgtttt tagacaagct gcgcgatttg tttgattgat tttaaggttc ggaaacaagc
     1501 agccgtatcg gggaatctcc ctgatacggc tgtttttgtt tgtttaaaaa tagtttttat
     1561 tttcaatggg gtatggggca gggtgggata actgttttta actgttcttt ttaaaacttg
     1621 acatcatggc gcgatgccaa caatatgtga acgtctgttg tcaaaggaag aataatgaat
     1681 aaatctttat ccgggtcggt agaagaatac cgcgaactga cgctccgagg catgatactc
     1741 ggtgcattga tcactgtaat ttttactgcg tccaacgttt acctcggttt gaaagtcggg
     1801 ctgacctttg cctcgtcgat tccggcggcg gtgatttcga tggcggtttt aaagtttttc
     1861 aaaggcagca atattttgga aaacaacatg gtgcagaccc aagcttcggc ggcgggtacg
     1921 ctttcgacca tcatcttcgt cctgcccggt ttgctgatgg cgggctactg gagcggtttc
     1981 ccgttctggc agacgacgct tttatgtatt gccggcggga ttttgggggt gattttcacc
     2041 attccgctgc gttacgcgat ggtggtgaaa agcgatttgc cttatccgga aggtgtggcg
     2101 gctgctgaaa ttttgaaagt gggcggtcat gaagaagggg ataaccgtca gggcggcagc
     2161 ggcatcaaag agctggcggc cggcggtgcg ttggcgggat tgatgagctt ttgcgccgga
     2221 ggtctgcgcg tgattgccga cagcgcgagt tattggttta aaagcggtac ggcgattttc
     2281 cagctgccga tgggcttttc actggcattg ttgggcgcgg gctatttggt cggactgacg
     2341 ggcggtatcg ccatcctgtt gggcatttcg attgcttggg gcattgccgt gccgtatttc
     2401 tcctcacaca ttccgcaacc ttccgatatg gaaatggcgg cgtttgcgat gaagctgtgg
     2461 aaggagaaag tgcgttttat cggtgcgggg actattggca ttgcggcggt ttggacgctg
     2521 ttgatgctgc tcaagccgat ggtggaaggc atgaagatgt cgttcaagag ttttggcggc
     2581 ggtgcgcccg ctgcggaacg cgccgaacag gatttgtcgc ctaaggctat gattttttgg
     2641 gtgctggcga tgatgtttgt tttaggcgtg tcgttttacc actttatcgg cgattcgcac
     2701 attacgggcg gcatggcttg gcttttggtg gtcgtttgca cgcttttggc ttccgtcatc
     2761 ggctttttgg tcgccgccgc ctgcggttat atggcaggtt tggtcggctc gtcttccagc
     2821 ccgatttccg gcgtgggcat cgtgtccgtc gtcgttattt cactggtttt gctgctggta
     2881 ggcgaatccg gaggtttgtt ggcggatgag gctaaccgca aatttttgct ggcactgact
     2941 ttgttttgcg gctcggcagt aatctgcgtg gcttcgattt ccaatgacaa cctgcaagac
     3001 ttgaaaaccg gctacctgct caaagccacg ccttggcggc agcaagtcgc cctgattatc
     3061 ggctgtatcg ttggtgcgct ggttatttcg cccgtgttgg aactgcttta cgaagcctac
     3121 ggctttaccg gcgcaatgcc gcgcgaaggc atggatgcgg cgcaggcttt ggcagcccct
     3181 caagcgactt tgatgacgac catcgcgtcg ggcattttcg cccacaacct tgaatgggtc
     3241 tatatcttta ccggcatcgc gattggcgcg gtgttgattg ccgccgattt ggtgttgaaa
     3301 aaatcatcag gcggcaaact tgccctgccc gtccttgcgg tcggtatggg tatttatctg
     3361 ccgccgtccg tcaatatgcc catcgtggca ggcgcggtgt tggcggcggt gttgaaacac
     3421 atcatcggta aaaaagcgga aaaccgtgaa ggccgtctga aaaacgccga gcgcatcgga
     3481 accttgttct ccgccggtct gattgtcggt gaaagtctga ttggtgtgat tatggcgttt
     3541 attattgcct tctccgtgac caacggcggc tcggacgcgc cgctcgcgtt gaatctgcaa
     3601 aactgggatg ccgccgcttc ttggctgggc ttggcgttct tcgttaccgg gatgtttttc
     3661 tttgcacagc gcgtactgaa ggcgggtagg tagcctgtcg gagaaaatgc cgtctgaaac
     3721 gttcagacgg cattttttat cgggaaagtg taaggcggag cttttcggct tgcgcccacg
     3781 ttttgccggc aaggtcttt
//
LOCUS       DAWFIE010000118         3751 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWFIE010000118 DAWFIE010000000
VERSION     DAWFIE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.119"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(166..279)
                     /locus_tag="WGQ71_001945"
                     /pseudo
     CDS             complement(166..279)
                     /locus_tag="WGQ71_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            418..493
                     /locus_tag="WGQ71_001946"
     tRNA            418..493
                     /locus_tag="WGQ71_001946"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:452..454,aa:Glu,seq:ttc)
     gene            complement(1004..1201)
                     /locus_tag="WGQ71_001947"
     CDS             complement(1004..1201)
                     /locus_tag="WGQ71_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707893.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
     gene            1262..2068
                     /gene="thiD"
                     /locus_tag="WGQ71_001948"
     CDS             1262..2068
                     /gene="thiD"
                     /locus_tag="WGQ71_001948"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ0707894.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVRTAKSYITAAISSPLEIGAGHGPVNHWAYRD"
     gene            complement(2118..2987)
                     /gene="tehB"
                     /locus_tag="WGQ71_001949"
     CDS             complement(2118..2987)
                     /gene="tehB"
                     /locus_tag="WGQ71_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ0707895.1"
                     /translation="MKEWIVGQSGELFCFGQMPVWKAENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELDEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            3101..3538
                     /gene="rnhA"
                     /locus_tag="WGQ71_001950"
     CDS             3101..3538
                     /gene="rnhA"
                     /locus_tag="WGQ71_001950"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ0707896.1"
                     /translation="MDKTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
BASE COUNT          929 a         1086 c          894 g          842 t
ORIGIN      
        1 cgtctgaaaa tttaaaagtt ctagattccc gctttcgcgg gaatgacgaa gtttcagacg
       61 gcatcgcccg cctgttttga tatggcggca cccccccccc cgacaaaaaa aacaatccgg
      121 aacgcatctg accgttccgg cttattttca ggcgaatccg ccgcatcaga acatactgcg
      181 cacgcccata ttgacctgcc aagtctggcg catcgtgtgc atcgaagacc tttgcgcctc
      241 aaaataaagc tgccttccgt tgtcggcatt accacgcaaa aaaatgaatt gcttgatatt
      301 ccaatgtttt tatatgtttt tatatggtga tgcgatcaga caaacgcccc cctgacattt
      361 gtttagacgg catcgtattg ctaaatttct ataagtatgt ataatgtccg tttccacgcg
      421 cccatcgtct agaggcctag gacactgccc tttcacggcg gcaaccgggg ttcgaatccc
      481 cgtgggcgtg ccaattcaaa aacctgcttg tttcaagcag gtttttttat tatgaatcgt
      541 cattcccgca actcttcgtc attcccgcaa aagcgggaat ctagagcgta gggttgaaga
      601 aaccgtttta tccgataagt ttccgtgccg acaggtctgg attcccactt tcgtgggaat
      661 gacgagtctt ttataacctt taaggctgct ctgcccaatg gtttggattc ccgcttatgc
      721 gggaatgacg gggcggggcg atgccgtctg aaacttcgtc attcccacaa cccttcgtca
      781 ttcccgtgaa aacgggaatc tagaacttct gaattttcag acggctttat aaatattgcc
      841 gctgccccaa gggctagatt cccgcctgcg cgggaatgat gatttggaaa tcacccgaaa
      901 cccaaaaaca actgaaaccg aacacactgg attcccgctt tcgtaggaat gacggaattt
      961 taggtttctg tttttgtgga aatgacgaat aaagcgtgcc ggtttatgct cgccgcaaca
     1021 cgcggttcag acggcattgc tctctttttt cattatcagt gggtgtagca actgtatttt
     1081 tcaccccgtc gggcaaaaat acagttgcta cgatgcaccc cgccgccctg ccctgtgcct
     1141 tgtcctgcaa tacggcatat aatgcaccac aaacccccgc gctgcggttt tcagacggca
     1201 tcgccgtgct tttttacagg cattagccct ttttatcgga cacaatatta aggaggaaca
     1261 aatgaaaagc tcttttgtgc aaacgcttac catcgccggt tcggattcgg gcggcggtgc
     1321 gggcattcag gcggatttga aaacgtttca gatgcgcggc gtgttcggaa cgtgcgtcat
     1381 caccgccgtt accgcgcaaa ataccttggg cgtgtcggcg gttcatctcg tcccgaccga
     1441 aaccatcacc gcacaaatcc aagcaatcag ggaagacttt gacatccgcg cctacaaaat
     1501 aggaatgctc ggcacggcgg aaatcatcga atgcgttgcc gacaagctga aacactgcga
     1561 cttcggcagg cgcgtactcg accctgtgat gattgccaaa ggcggtgcgc cgctgttgca
     1621 ggattccgcc gttgcggcac tgacgcgcct gctgcttccc gatacggatg tattgacccc
     1681 caacctgccc gaggcggaag ccctgaccgg cgtgcatatc gaaaaccgta aagatgcgga
     1741 acgtgcggca aaaatcctgc ttgattacgg tgtcaaaaat gtcgtcatca aaggcggaca
     1801 tttgaatggc agcagcagcg gacgctgcac ggattggctg tttacgcaaa acgaaacgct
     1861 ggaattggac agcccgcgct ttccgaccgc ccacacgcac ggcacgggct gcacgttttc
     1921 tgcctgcatt accgccgagt tggcaaaagg cttagacgtt tgcaaagccg tacggactgc
     1981 caagtcctac atcacggcgg caatctcaag ccctttggaa atcggcgcgg gacacggccc
     2041 ggtcaatcat tgggcctatc gggactaacc gtaaaaatgc cgtctgaaac aaaatgttca
     2101 gacggcattt ttgaggatta ttcaggcttt ttcgccagca tcgttacaaa tttaaaccgt
     2161 atcggattgc cgttttcgtc tttggcgtgc atcgcgccca attcttcttt atattcgtcc
     2221 agctcccaat cccgataata atccttcagc tctccctctt taaatttaaa agggaacggc
     2281 atcggacaag ggaaatccgc cgtatccatt gccgatacga ttaagttgta cccgcccgcc
     2341 gccgtatgcg cctgcatatc ggcaatcacg tcaggcacgc gctgcggcat caggaacatc
     2401 agcaccactg ttgccacaat ataatcaaac tcaccctgca aggcggcggc gttcaaatca
     2461 tattccagcg tgcggacgtt caaaccctcc gcctctgcca gctccgccac gtttgccaag
     2521 gcggcgggat tgtgatcgac tgcagtaact tcaaatccct tcaaaccgag aaacagtgcg
     2581 ttgcgcccct gtccgcagcc catatccaac gccctgcccg ccggtacggt atcctgagcc
     2641 gccgcaaccg cagaatgcgt ggcactcatg ccgtattttt tgtgaaaata gtctgccgcc
     2701 gcgcaataca gcgacaaacg gatttcggca tcgtccgttt tcggtttgac agaaaacacc
     2761 tgctgcggcg caaacacgca atcgccgctt tctgccgacc aaacctctgc cgccccgtcc
     2821 tgcgtgcgga cttcgacatc gccctgcaac acattcaggc agacccactc cccttcctca
     2881 gacgaatagc ccgacaacaa aacttccggc aggttttccg ccttccatac gggcatctgt
     2941 ccgaaacaaa acaactcgcc gctttgaccc actatccact ccttcatatt caaaaataaa
     3001 gttgcacatt atatgcctat tttaatccgc cgcaatcttt cagacggcac ggcgcacaaa
     3061 ccgcttataa tcacgccaga caccacacaa aggcacaata atggacaaaa ccgtttacct
     3121 ttacaccgac ggcgcgtgca aaggcaatcc cggcgcgggc ggctggggcg tgttgatgcg
     3181 ctacggcagc cacgaaaaag aacttttcgg cggcgaagcg caaaccacca acaaccgcat
     3241 ggagctgacc gccgtcatcg aaggactgaa atcgctcaaa cgccgctgca ccgtcatcat
     3301 ctgcaccgac tcgcaatacg tcaaaaacgg catggaaaac tggatacacg gttggaagcg
     3361 caacggctgg aaaaccgccg ccaaacagcc cgtcaaaaac gacgacttgt ggaaagaact
     3421 cgacgttctg gttgggcagc accaagtcag ctggacttgg gttaaaggac acgcgggaca
     3481 cgcggaaaac gaacgcgccg acgatttggc aaaccgtggc gcagcgcagt tttcctgact
     3541 gccgctccgg caaaaatgcc gtctgaaacc gctaatgggc tttagacggc atcgtcctcc
     3601 accgtcattc ccacgcaggc gggaatccaa accgtcgggc aacagcaata ttcaaagatt
     3661 atctgaaagt ttgaagttct agattcccat tttcacggga atgacgaaaa gttgcaggaa
     3721 tgacggagtt tcaggcggca tccgaccgcc c
//
LOCUS       DAWFIE010000119         3728 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWFIE010000119 DAWFIE010000000
VERSION     DAWFIE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.120"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            1523..3658
                     /locus_tag="WGQ71_001951"
     CDS             1523..3658
                     /locus_tag="WGQ71_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0707897.1"
                     /translation="MNAPLFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFAGNDGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGYKSPNGGKT
                     VPYSALDKRSYLAKIGTSFGDGDHRIVLSHMKDQHRGIRTVREEFTIFDSDPKKDRQK
                     PSYRETTQSNTNLEYTGKNLGFVEKLNANAYVLEKKRYSADDSGSGYAGNVEGPNHTR
                     ITTRGANFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGEFKIKDKDDATEQDKQKNR
                     EDEKIAKAYRLTNPTKTDAGVYVEAIHDIGDFTLTGGLRYDRFKVKTHDGKTVSNSSL
                     NPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTKAERARNTEI
                     GFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIKNHGYELGASYRT
                     GGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFKNPNLEIGWRG
                     RYVQKAVGSILAAGQKNRDKQLENVVRKGFGVNDVFANWKPLGKDTLNVNFSVNNVFN
                     KYYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT         1003 a         1007 c          849 g          869 t
ORIGIN      
        1 ccttagaaca acagcaatat tcaaagatta tctgaaagtc cgggattcta gattcccact
       61 ttcgtgggaa tgacgaattt taggtttctg tttttggttt tctgtctttg tgggaatgat
      121 gaaattttaa gttttaggaa tttatcggaa aaaacagaaa ccgctccgcc gtcattcccg
      181 cgcaggcggg aatctaggtc tgtcggtgcg gaaacttatc ggataaaacg gtttcttcag
      241 attttacgtt ctggattccc actttcgtgg gaatgacgaa ttttaggttt ctgtttttgg
      301 ttttctgtcc ttgcgggaat gatgaaattt taagttttag gaatttatcg gaaaaaacag
      361 aaaccgctcc gccgtcattc ccgcgcaggc gggaatctag aatttcaatg cctcaagaat
      421 ttatcggaaa aaaccaaaac ccttccgccg tcattcccgc aaaagtggga atctagaaat
      481 gaaaagcagc aggaatttat cggaaatgac cgaaactgaa cggactggat tcccgctttc
      541 gcgggaatga cggcgacagg gttgctgtta tagtggatga acaaaaacca gtacgtcgtt
      601 gcctcgcctt agctcaaaga gaacgattct ctaaggtgct caagcaccaa gtgaatcggt
      661 tccgtcctat ccgtactgtc tgcggcttcg ccgccttgtc ctgatttccg ttcgttttcg
      721 gttattcccg ataaattacc gccgtttctc atcatttctt taacccttcg tcattcccgc
      781 gcaggcggga atctagtttt tttgagttcc agttgtttct gataaattct tgcagctttg
      841 agttcctaga ttcccgcttt cgcgggaatg acgcggaaaa gttgcttttc gtcattcccg
      901 cgcaggcttc gtcattcccg cgcaggcggg aatctaggtt cgttcggttt cggttttttc
      961 cgatagattc ctgccgcgtt aggggttcta gattcccgct ttcgcgggaa ggacgcggaa
     1021 aggttgcttt tcgtcattcc cgcgcaggcc tcgtcattcc cgcacaggct tcgtcattcc
     1081 cgcgcaggcg ggaatccagt ctgttcggtt tcagtcattt ccgatagatt cctgccgcgt
     1141 taggggttct agattcccgc ttttgcggga atgacgcgga aaggttgctt ttcgtcattc
     1201 ccgcatttgt taatccacta tatttccgcc gttttttata gtggactaac aaaaaccagt
     1261 acagcgttgc ctcgccttgc cgtactatct gtactgtctg cggcttcgtc gccttatcct
     1321 gatttttgtt agtccactat acatttccga caaaacctgt caacaaaaaa caacacaatg
     1381 caaataaaaa cgataatcag ctttgcaaaa atcccccccc cctgttaata taaataaaaa
     1441 taattattaa ttatttttct tatcctgcca aaccttaacg gtttggattt actttccttc
     1501 atatactcaa aaggacgact gaatgaacgc cccattgttc cgcctcagcc tgctctcgct
     1561 cacacttgcg gcaggctttg cccacgcggc agaaaataat gccaatgtcg cattggatac
     1621 cgttaccgta aaaggcgacc gccaaggcag caaaatccgt accaacatcg ttacgcttca
     1681 acaaaaagac gaaagcaccg caaccgatat gcgcgaactc ttaaaagaag agccctccat
     1741 cgatttcggc ggcggcaacg gcacgtccca attcctgacg ctgcgcggca tgggtcagaa
     1801 ctctgtcgac atcaaggtgg acaacgccta ttccgacagc caaatccttt accaccaagg
     1861 cagatttatt gtcgatcccg ctttggttaa agtcgtttcc gtacaaaaag gcgcgggttc
     1921 cgcctctgcc ggtatcggcg cgaccaacgg cgcgattatc gccaaaaccg tcgatgccca
     1981 agacctgctc aaaggcttgg ataaaaactg gggcgtgcgc ctcaacagcg gctttgccgg
     2041 caacgacggc gtaagctacg gcgcaagcgt attcggaaaa gagggcaact tcgacggttt
     2101 gttctcttac aaccgcaacg atgaaaaaga ttacgaagca ggtaaaggct acaaaagtcc
     2161 caacggcggc aaaaccgtac cgtacagcgc gctggacaaa cgcagctacc tcgccaaaat
     2221 cggaacaagc ttcggcgacg gcgaccaccg cattgtgttg agccatatga aagaccaaca
     2281 ccgaggcatc cgcactgtgc gtgaagaatt tacaatcttc gattccgatc ctaaaaaaga
     2341 caggcaaaaa ccctcatacc gcgaaaccac acaatccaac accaacttgg aatatacggg
     2401 taaaaacctg ggctttgtcg aaaaactgaa cgccaacgcc tatgtgttgg aaaagaaacg
     2461 ctattccgcc gatgacagcg gcagcggtta cgcaggcaat gtagaaggcc ccaaccatac
     2521 ccgaatcacc actcgtggtg cgaacttcaa cttcgacagc cgccttgccg aacaaaccct
     2581 gttgaaatac ggtatcaact accgccatca ggaaatcaaa ccgcaagcgt ttttgaacgg
     2641 cgaatttaaa ataaaagata aagatgacgc aaccgagcaa gacaagcaaa aaaaccgtga
     2701 agatgaaaaa attgccaaag cctaccgtct gaccaaccca accaaaaccg atgccggcgt
     2761 atatgttgaa gccattcacg acatcggcga tttcaccctg accggcgggt tgcgttacga
     2821 ccgcttcaag gtgaaaaccc acgacggcaa aaccgtttca aacagcagcc ttaacccgag
     2881 tttcggcgtg atttggcagc cgcacgaaca ctggagcttc agcgcaagcc acaactacgc
     2941 cagccgcagc ccgcgcctgt atgacgcgct gcaaacccac ggtaaacgcg gcattatctc
     3001 gattgccgac ggcaccaaag ccgaacgcgc gcgcaatacc gaaatcggct tcaactacaa
     3061 cgacggcacg tttgccgcaa acggcagcta cttctggcaa accatcaaag acgcgcttgc
     3121 caatccgcaa aaccgccacg tctctgccgc cgtccgtgaa gccgtcaatg ccggttacat
     3181 caaaaaccac ggttacgaat tgggcgcgtc ctaccgcacc ggcggcctga ctgccaaagt
     3241 cggcgtcagc cacagcaaac cgcgctttta cgatacgcac aaagacaagc tgttgagcgc
     3301 gaatcctgaa tttggcgcac aagtcggccg cacttggaca gcctcccttg cctaccgctt
     3361 caaaaacccg aatctggaaa tcggctggcg cggacgctat gttcaaaaag ccgtgggttc
     3421 gatattggcg gcaggtcaaa aaaaccgcga caaacaattg gaaaacgttg tacgcaaagg
     3481 tttcggtgtg aacgatgtct tcgccaactg gaaaccgctg ggcaaagaca cgctcaatgt
     3541 caatttttcg gttaacaacg tgttcaacaa gtactactat ccgcacagcc aacgctggac
     3601 caataccctg ccgggcgtgg gacgtgatgt acgcttgggc gtgaactaca agttctaaaa
     3661 cgcacatccc gaaaaaatgc cgtctgaaag cctttcagac ggcatctgtt ctgataattt
     3721 gatatata
//
LOCUS       DAWFIE010000120         3632 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWFIE010000120 DAWFIE010000000
VERSION     DAWFIE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.121"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..116)
                     /locus_tag="WGQ71_001952"
     CDS             complement(<1..116)
                     /locus_tag="WGQ71_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0707898.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKNTAA"
     gene            377..1849
                     /gene="pyk"
                     /locus_tag="WGQ71_001953"
     CDS             377..1849
                     /gene="pyk"
                     /locus_tag="WGQ71_001953"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ0707899.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            2159..3559
                     /locus_tag="WGQ71_001954"
     CDS             2159..3559
                     /locus_tag="WGQ71_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ0707900.1"
                     /translation="MTPSALKKTVLLLGTAFAAASVHASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWS
                     TMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFE
                     KEKTVVKGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMN
                     SLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARF
                     KNHADIIGLQYTYKFK"
BASE COUNT          933 a         1086 c          913 g          700 t
ORIGIN      
        1 gctgcggtat ttttattaac gcctactaac tctgctgccg ttcttgcagt tacacctgcg
       61 acaaatagct caatgagttt attttgttta taccggctta gacgactttt tctcataggg
      121 ataattctaa cttaatttga atttccctag ttatctagga cagccccaaa aataaattta
      181 gcccaagtca taaaaccgtg taattttgca ccaaaaaagg cggtttgcag gtaaaatatc
      241 aaactattaa tttaaatcat atttttacag aatattccgc cgcgttcatc aaatggacat
      301 caaaccggtt ccaaatttct gtaattttgt aacaaaatac cgcaaaacac ccgattgaga
      361 ccaaaaggac tttcatatga accagacaag ccgcgatctg acccgcatca gccacaacac
      421 caaaatcgtc gccacccttg ggccgggcag caacaacgta gaactgttgg aagatatgat
      481 ccgcgtcggc ggcctgaacg tcgtccgctt caacttcagc cacggcacgc ccgaattcca
      541 tcaggaaaac gcccgcatcg tgcgcgaggc ggcaaaacgc gccggacagg aaatcgccat
      601 cattgccgac ctgcaagggc cgaaaatccg cgtgggcaaa atcgccggcg gcggcattga
      661 attgaacaaa ggcgaaacgc tggtactgga tgccgcgctc gaaggcgaag gcacgcgcga
      721 ggcggtcggt ttggactacc gcgacctgcc cgacgacgtt gccgcaggcg acgtattgtg
      781 gctggacgac ggcctgctga ccctgaccgt ggaatccgtc gaaggcagca ggattatcac
      841 aagggtggaa aacagccacg tcctgaaaag caacaagggc atcaacaaac gcggcggcgg
      901 tctgtccgca ggcgcgttga ccgaaaaaga cttccgcgac ctgaaaaccg cgattgccat
      961 cggttgcgac tacctcgcca tcagctttgt gaaatccgcc gaagatttgc atatcgcgcg
     1021 cgccaaagtc gaagaagaaa tgaagggcag cacggccgtg cgccccggtt tggtttccaa
     1081 aatcgaacgc gtggaagcga ttgaaaactt ggacgaaatc atccttgccg gcgacggcat
     1141 tatggttgcg cgcggcgact tggcggtcga agtcggacac gccgccgtcc ccgccctgca
     1201 aaaacggatg atccgccgcg cccgcgagtt gcgccgcttc agcattacgg cgacgcaaat
     1261 gatggaatcg atgattacca accccgtgcc gacccgcgcg gaagtcagcg atgtggcaaa
     1321 cgcggtattg gacggcaccg atgcggtgat gtgttccgcc gaaaccgccg tcggcgcgta
     1381 tccgtttgaa accgtcagcc aaatggcaat tatctgcgcg gctgcggaaa aagagcagga
     1441 ttcgctcaac ggcgttgccg aacaggcgga gtatcccgaa gcggtcagca ccaacctggc
     1501 ggttgccggc ggcgcggtca gcgtggcgcg cgcggttcac gccaaagcca tcgtcgccct
     1561 gaccgaaagc ggttcgaccg cctttgaaat cagccgccac aacatcaccc tgccgatttt
     1621 cgcgctgacc ccgagcgttt ccgcccaacg ccgtatggcg atgtaccggg gcgtgcgccc
     1681 gctgattttg gcaaccagca ccgaccacga cacggcgtta aacgaagtcg aaaccatgct
     1741 ggtggaacac aacatcctgc attccggcga ccaatacatc attaccagcg gctcgcaaat
     1801 gcgcgaatcc ggttcgacca atacgctgga agtgctgcgc gtcaaataat ccgccctgag
     1861 tggaaaatgc cgtctgaagt cgatgcccga ggcttcagac ggcatttttt gcggcggcgc
     1921 ggcggttcgg gcaaactctg aaaaaattgt gcaaatccgg caacatcgga taaaatcgag
     1981 tacctatact aaagcgaaac aaggcatttc cgactgcctt ttttatagtg gattaaattt
     2041 aaaccagtac ggcgttgcct tgccgtacta tctgtactgt ctgcggcttc gtcgccttgt
     2101 cctgattttt gttaatccac tatatttatc caccgtccgc ctttttacgg aaaccgaaat
     2161 gaccccttcc gcactgaaaa aaaccgtcct actgctcggc actgcctttg ccgccgcatc
     2221 cgtccacgca tccggctacc acttcggcac acagtcggtc aacgcgcaaa gcacggcaaa
     2281 tgccgccgcc gcagaagccg ccgacgcatc gaccatcttc tacaaccctg ccggcctgac
     2341 caaactcgac agcagccaga tttccgtcaa cgccaacatc gtgctgccca gcattcatta
     2401 tgaggcggat tccgccaccg actttaccgg gcttcccgtc caaggttcga aaagcggcaa
     2461 aatcaccaaa accacggtcg cgccccacat ctacggcgca tacaaagtca acgacaatct
     2521 gaccgtgggc ttgggcgtgt acgtcccctt cggctctgcc accgaatacg aaaaagattc
     2581 cgtgttgcgc cacaacatca acaaactcgg tctgaccagc atcgccgtcg aacctgtcgc
     2641 cgcgtggaaa ctcaacgacc gccattcctt cggcgcaggc atcatcgccc aacatacttc
     2701 cgccgaactg cgcaaatatg ccgactgggg gattaagagt aaagcagaga tattgacggc
     2761 aaaaccgccc aaacctaacg gtgtagccga agctgcaaaa attcaggccg acggacacgc
     2821 cgatgtcaaa ggcagcgatt ggggcttcgg ctaccaactg gcgtggatgt gggacatcaa
     2881 cgaccgtgcg cgcgtgggcg tgaactaccg ttccaaagtc tcgcacacgc tcaaaggcga
     2941 tgccgaatgg gcggcagacg gcgcggcggc gaaagcaatg tggagtacga tgcttgcagc
     3001 aaacggctac acggcgaatg aaaaagcccg cgttaaaatc gttacgcctg agtctttgtc
     3061 cgtacacggt atgtacaaag tgtccgataa agccgacctg ttcggcgacg taacttggac
     3121 gcgccacagc cgcttcgata aggcggaact ggtttttgaa aaagaaaaaa ccgtcgtcaa
     3181 aggcaaatcc gaccgcacca ccatcacccc caactggcgc aacacctaca aagtcggctt
     3241 cggcggttct tatcaaatca gcgaaccgct gcaactgcgc gccggcatcg cttttgacaa
     3301 atcgcccgtc cgcaacgccg actaccgcat gaacagccta cccgacggca accgcatctg
     3361 gttctccgcc ggtatgaaat accatatcgg taaaaaccac gtcgtcgatg ccgcctacac
     3421 ccacatccac atcaacgaca ccagctaccg cacggcgaag gcaagcggca acgatgtgga
     3481 cagcaaaggc gcgtcttccg cacgtttcaa aaaccacgcc gacatcatcg gtctgcaata
     3541 cacctacaaa ttcaaataaa cggcccgccg tttgaatgta acaatgccgt ctgaaacaga
     3601 tttccgtttc agacggcatt gttttaggct cg
//
LOCUS       DAWFIE010000121         3485 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWFIE010000121 DAWFIE010000000
VERSION     DAWFIE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.122"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(231..1295)
                     /locus_tag="WGQ71_001955"
     CDS             complement(231..1295)
                     /locus_tag="WGQ71_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0707901.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRDCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLYALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTDRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIVEHHQSGILLDRLTPESLADEIERLFLNPETKNALATAAH
                     QCVAARFTINHTADKLLDAI"
     gene            complement(1296..2054)
                     /locus_tag="WGQ71_001956"
     CDS             complement(1296..2054)
                     /locus_tag="WGQ71_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0707902.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDYSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAKKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2129..2204)
                     /locus_tag="WGQ71_001957"
     tRNA            complement(2129..2204)
                     /locus_tag="WGQ71_001957"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2169..2171),aa:Ile,seq:cat)
     gene            complement(2258..3394)
                     /locus_tag="WGQ71_001958"
                     /pseudo
     CDS             complement(2258..3394)
                     /locus_tag="WGQ71_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            complement(3339..>3485)
                     /locus_tag="WGQ71_001959"
     CDS             complement(3339..>3485)
                     /locus_tag="WGQ71_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0707903.1"
                     /translation="NHSKEFADRQNHINGIENFWNQAKRVLRKYSGLARTFPRIGSAT
                     GCAD"
BASE COUNT          791 a          841 c          920 g          933 t
ORIGIN      
        1 gtactggttt ttgttagtct actatacatt gtaacaaagc acttgacgcg gcagcacaag
       61 ccggggaaga cgctgcaaaa ctttgccaat aggctgcgta atatcggggg atattgggat
      121 tttcttattt tgaaggatga attcggggtg tttctttcca atgaacgaat ataatttagt
      181 ggtttgttgc atcaaaggct ttcagacggc atatgccgtc tgaaagcagt ttatattgca
      241 tccaataatt tgtcggcggt atggttgatg gtaaaacggg cggcgacgca ttgatgagct
      301 gccgttgcca gtgcgttttt cgtttcaggg ttcaagaaga ggcgttcgat ttcgtccgcc
      361 aaagattcag gtgtcagcct gtccagcaga atccccgatt gatgatgttc gacaatttcc
      421 ttttgcgccc ccaaagtatt ggaaatcacc gccgttcggc agtacatcgc ctcgcataat
      481 gacaaaccga atgcctccgg gacgaggctg ggcaaaacca cgacatcgct ttggcggtaa
      541 aacgaagcaa gtttttcggt aaacccttca aaagaaacaa atggttctgc tcctgaagca
      601 gatacgtccc gcttcaggcg gcacatataa tccggatgtc cgtcccctgc caatttgagc
      661 ctgatttgag gatatttccg atgcagtatc acacaggctt caatcaggtt ttccaatcct
      721 ttttccggac tgatcctgcc ggcgtaggcg acggtaaaaa aacggctgtc gggtttttct
      781 tgagagggag ggaagcgatc ggtatcgata ccgttatgaa caatccggta tttttcttta
      841 aagggattgt cggcggtttg cacatcgtaa accagacggg aaacgcaaat aaagcggtcg
      901 gtgtttttct gtatcaggcg gtgataaaaa tcagttttgt tggcgacaac attatgtttg
      961 acaaatatca ggcgcacccc ggtcagtttt ttcaaaagta tggataaggc ggcaattttg
     1021 ccggtgtgta tcatcaggtg ggaaatgcgg tttttccgga taaagcgggt aagtgcgtag
     1081 agcgaaaaca gcccgttgag cgtgtgaagg cgcgttgtga aaacggaaga aacttcggaa
     1141 aatcgcctgt gcatcaattc attattttta ttgacggcgg taaacattgt gcagtcccga
     1201 agccccaatg cttttgaaac atcatagaca tattgttcgc ctccacccca aatcttggcc
     1261 gatacgatat ttaatatcct gaattctttt tccatttaaa attttccgcc ggatttgtac
     1321 agataatata gtttgacata tttaatcatc gtgtaatagc tgtggttgac ggacataatc
     1381 caacccattt ttccatcaag aaaccctttg ttcaggatat aaattttgaa aaacccccaa
     1441 atcgggcgga ggataatgtc cctaacgaaa cgcacgggct ttccctgctc tcggtatttt
     1501 ttggctgaaa tggaagtata tttgttgaac ttgttgaaat attgttccca gttgtcgtac
     1561 gtgtaatggt acataaaatg cttcagacgg cgtttggggt agggggtttg tacggtttcg
     1621 tgtactttgc cttgcaccga actgtctttt ttcggcatca gacggcatac gctgtcggga
     1681 cgcatcgcgc cgtgtgtggc gggatggttg gggaaaaggt tgcggcgttc gacaaaatag
     1741 gcggcataat cgccggtttg gacaattttt gagatttcat cagatagttc cggcgtgcag
     1801 cgttcgtctg catcaatcag gaaaacccat tctccgcctg cctgttcgat ggcaaatgtt
     1861 ttttgcgctc cgaaatcccc attcaaatgc cgtctgaaga cttttgcgcc caaaccctcg
     1921 gcaatttcgg cagtattgtc ggtgctgtag tcgtcgataa cgatgacttc tttatcgaaa
     1981 cggcaacttt caatacattc ctgaatgtgg tttgcttcgt ttttggcaac aatcaatacg
     2041 ctgacttttt tcattatcaa ggtttctcaa agcattttgg tttcacaggg cggcaaaaaa
     2101 gcccaaacat acgtccgggc ttgggaattg gtgggtccgg tggggttcga acccacgacc
     2161 aagggattat gagtcccctg ctctaacccc tgagctacag acccgtcaag agtaggcgcg
     2221 tattgtatag catagtggtg ggattgacaa acttatttca tccgatgatt aagacgcagt
     2281 tgcttcctcc gaaggcaaac gacgagctgg cggcaatgtg tttttccgtt gtctagaagc
     2341 tgccgctgcc ggtcaggtta atggcgggca ggtcggggtc gttctgcccg tcccaaagct
     2401 ggggcggaag ttttccttcg ggattgcttt gccggtcggc aaagccccac gcgaacgcgg
     2461 cttcgattgc gcccgccgca cccagcgtgt gtccggtttg cggcttggtg gacgtgcagg
     2521 gcgtattgtt gccgaaaacc gctgcaacgg cacggctttc catactgtcg ttgtggtgcg
     2581 tcccggtgcc gtgcagatta atccagccga tgtcttcggg cgcaagacct gcgtgttgca
     2641 atgccgtctg aaaggcaagg attgcgcctt gcgcgtcggg gcggggcgtg gaaatatggt
     2701 aggcatcgct gttggctccg tagccgacga gttcggcgta aattatcgca ccgcgccgtt
     2761 tggcgtgttc caattcttcc agcacgaaaa tccccgcgcc ttcgccgatg accagcccgt
     2821 cgcggttcgc gtcgtatggg cgcggggttt tttccggttc gccgttgcgg cggctggcgg
     2881 cataaagcga gtcgaaaaca tacacttcgg acgggaaaaa ttcttcgcct ccgcccgcca
     2941 gcatcatatc ggtcagaccg tatttgatgg cttcgtaggc ataacctatg ccttggctgc
     3001 cggacgaaca cgcgctcgat gtcgggatga tgcgtccttt cagtccgaaa aagatgccga
     3061 tattggcggc ggcggtgtac ggcatcatac gcacataggt gttggcactg aagttgcgcg
     3121 acatgccggt cagcaacaat tcgcctacat cgccgatgtc tttggtgctg ccgctggaag
     3181 agccgcaggc aacgcccatc cgtccgtcgg taatgctttc gtccccgagc aaaccggcat
     3241 ccgtcaaagc ctgctccgcc gcatcgacgc acaaatacga cacacgcccc atacttctga
     3301 gctgcttgcg cgtccaatgt ttcggcggcg cgtaatcctc aatcggcgca cccagttgcg
     3361 cttccaattc ggggaaacgt tcgtgccaat ccactatatt ttcgcaagac gcgttttgcc
     3421 tgattccaaa agttctcaat gccgttaatg tggttctgac ggtctgcaaa ctccttggaa
     3481 tggtt
//
LOCUS       DAWFIE010000122         3400 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWFIE010000122 DAWFIE010000000
VERSION     DAWFIE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.123"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            202..3198
                     /locus_tag="WGQ71_001960"
     CDS             202..3198
                     /locus_tag="WGQ71_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ0707904.1"
                     /translation="MNAQKLPEVKWGSSYNSLSVKSNEREVTHTSSLGIRKNVSFLFN
                     NTDQVVAEKKDAVVFGAATYLPPYGKVSGFDVVKLTERNNAVDWIGTTHPGLIGYSYE
                     NSTCSSNNCPEVNYRTQFTFNSNELKKNGNDGKLDIYEDKSRDNSPIYKLKDHPGLGV
                     SFNLGGESSFKPKRNSQLVSSFRENVQNNQYKENLVYTTDDYNNKNNRNHQDKHHAVA
                     FYLNAKLHLLDKKQIKNIAKGSELKLGELKTRIEPTDRWKNQNGNFFNGGTWTYEDKG
                     VVSVTLKLPQVKADRCINKPNPNKNSKALSPALTAPALWFGPVQNGKVQMYSASVSTY
                     PDSSSSRIFLQNLERKTDPGRPGRHSLKPLNEAQIKSKEPSFTSRQTIIRLDGGVQQI
                     KLGKNNNEVTGFNGNSNNATFGIVQEYGFKPNDDEWKKVLLPWTVRGSADDNRFKSIN
                     QESSKYSQRYRIRDNGNRDLGDIVNSPIVAVGEYLATSANDGMVHIFKKGNGDARNYS
                     LKLSYIPGTMPRKDIESKESTLAKELRAFAEKGYVGDRYGVDGGFVLRQVERDGKTRV
                     FMFGAMGFGGRGAYALDLTKADGSDPKQASLFDVKHDNNGKNSNNSVQLGYTVGTPQI
                     GKTHDGKYAAFLASGYATKRIDDPTNKTALYVYDLESSGTLIKKIEVPSGKGGLSSPT
                     LVDKDLDGMVDIAYAGDRGGSMYRFDLSGNDPTKWSARAIFSGNKPITSAPAISQLKD
                     KRVVIFGTGSDLSEEDVDNMEEQYIYGIFDDDTETTGSVNFSGSGGGLLEQVLSRDND
                     NKTLFLTDYKRSDGSGDKGWVVKLKDGQRVTVKPTVVLRTAFVTIHKYTGNDKCGAET
                     AILGINTADGGKLTKKSARPIVPDANTAVAQYSGHKKGTNGKSIPIGCMQKGNEIVCP
                     NGYVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTLL
                     MNDLDSLDITGPTCGMKRISWREVFY"
BASE COUNT          988 a          829 c          878 g          705 t
ORIGIN      
        1 atcgggcggt ttaccgaacc ccggcgttcg cggcgcgccg ccccgtgaag gcaaactcaa
       61 ggaataaaag atgaataaaa cttggaaacg gcaggttttc cgccataccg cgctttatac
      121 cgccatattg atgttttccc ataccggcgg cggggggggg gggggcaggc gcaagcgcaa
      181 acgaataaat acgctattgt aatgaacgcg caaaagctgc ctgaggtaaa gtgggggagt
      241 agttataact cattgtcggt caaaagcaat gaacgcgaag ttacccatac gagtagtttg
      301 ggaatcagaa aaaacgttag tttcttattc aataataccg atcaagttgt tgctgaaaaa
      361 aaagatgctg tcgttttcgg cgcggcgacc tacctgccgc cctacggcaa ggtttccggt
      421 tttgatgtcg ttaagctgac cgagcgcaat aatgctgttg attggattgg tacgactcac
      481 ccggggttga taggctacag ctacgaaaat agcacatgct ccagcaacaa ctgtcctgaa
      541 gttaactata gaacccaatt taccttcaat agtaacgagt tgaaaaaaaa tggaaacgac
      601 ggcaagctgg atatatacga agacaaaagc cgcgacaatt cgcccattta caaattgaag
      661 gatcatcctg ggttgggcgt gtctttcaat ttgggcggag agagctcctt caaaccaaag
      721 agaaacagtc aattggtatc ttcttttaga gagaacgtgc agaacaacca atacaaagaa
      781 aacctcgttt atacgacaga cgattacaat aataagaata atcgtaacca tcaggacaaa
      841 caccacgccg tcgcctttta tctgaacgcc aaactgcacc tgctggataa aaaacaaatc
      901 aaaaatatcg cgaaaggcag tgaacttaag ttgggtgagt tgaaaacgcg catcgagccg
      961 acggataggt ggaaaaacca gaatgggaac ttttttaatg gtggtacttg gacgtatgaa
     1021 gataaaggag tagtcagcgt cacactcaaa ttgccgcaag tcaaagcaga ccgctgcatc
     1081 aataaaccga accccaataa aaacagcaaa gccctttcgc cggccctgac cgcccccgcg
     1141 ctgtggttcg gacctgtgca aaatggtaag gtgcagatgt attccgcttc ggtttccacc
     1201 taccccgaca gttcgagcag ccgcatcttc cttcaaaatc tggaaagaaa aaccgacccc
     1261 ggcagacccg gccgccattc cctcaaaccc ttgaatgagg cgcagattaa aagtaaagag
     1321 ccgagtttca caagtcggca aacaatcatc cgattggatg gcggcgtgca gcagatcaaa
     1381 ctgggtaaaa acaataatga ggtcaccggt tttaatggaa acagcaacaa cgcaactttc
     1441 ggcattgttc aggaatatgg cttcaagcct aatgacgacg agtggaaaaa agtgctgctg
     1501 ccttggacgg ttcggggttc tgctgatgac aatcgattta aatcaattaa ccaagaatca
     1561 agcaaataca gccaaagata ccgcatccgc gacaacggca atcgcgattt gggcgacatc
     1621 gtcaacagcc ccatcgtggc ggtcggcgag tatttggcta cttccgccaa cgacgggatg
     1681 gtgcatatct tcaaaaaagg caacggggac gcgcgcaact atagtctgaa gctcagctac
     1741 atccccggaa cgatgccgcg caaagatatt gaaagcaaag aatccaccct tgccaaagag
     1801 ctgcgcgcct ttgctgaaaa aggctatgtg ggcgaccgct acggcgtgga cggcggcttt
     1861 gtcttgcgcc aagtcgaacg ggacgggaaa acccgcgtgt ttatgttcgg cgcgatgggc
     1921 tttggcggca gaggtgcgta tgccttggat ttaaccaaag ccgacggcag tgacccgaaa
     1981 caagcctctt tgtttgatgt caaacatgac aataatggca agaatagcaa taatagcgtg
     2041 caattaggct acaccgtcgg cacgccgcaa atcggcaaaa cccacgacgg caaatacgcc
     2101 gccttcctcg cctccggtta tgcgactaaa agaattgacg acccaacaaa taaaaccgcg
     2161 ctgtatgtat atgatttgga aagcagcggc acgctgatta aaaaaatcga agtacccagc
     2221 ggcaagggcg ggctttcgtc ccccacgctg gtggataaag atttggacgg catggtcgat
     2281 atcgcctatg ccggcgatcg cggcggtagt atgtaccgct ttgatttgag cggcaacgac
     2341 ccgaccaaat ggtctgcacg cgccattttc agcggcaaca aaccgattac ttccgcgccc
     2401 gccatttccc aactgaaaga caaacgcgtg gttatcttcg gcacgggcag cgatttgagt
     2461 gaagaggatg tggacaatat ggaagaacaa tatatttacg gtatcttcga cgacgatacg
     2521 gagacgacgg gttctgtaaa cttcagcggt tcgggaggcg ggctgcttga gcaagtgctt
     2581 agtagggata acgacaataa aaccttattc ctgaccgatt acaagcgatc cgacggatcg
     2641 ggcgacaagg gctgggtagt gaaattgaag gacggacagc gcgttaccgt caaaccgacc
     2701 gtggtattgc gtaccgcctt tgtaaccatc cataaatata cgggtaatga caaatgcggc
     2761 gcggaaaccg ccattttggg catcaatacc gccgacggcg gcaagctgac caagaaaagc
     2821 gcgcgcccga ttgtgccgga cgccaatacg gctgtcgcgc aatattccgg ccataagaaa
     2881 ggcaccaacg gcaaatccat ccctataggt tgtatgcaaa aaggcaatga aatcgtctgc
     2941 ccgaacggat atgtttacga caaaccggtt aatgtgcgtt atctggatga aaagaaaaca
     3001 gacggatttt caacaacggc agacggcgat gcgggcggca gcggtataga ccccgccggc
     3061 aagcgttccg gcaaaaacaa ccgctgcttc tcccaaaaag gggtgcgcac cctgctgatg
     3121 aacgatttgg acagcttgga cattaccggc ccgacgtgcg gtatgaaacg aatcagctgg
     3181 cgtgaagtct tctactgatt tgcacgcgaa aatgccgtcc gaaaggtttt cggacggcat
     3241 tttttgcgtt tttcgggagg ggcgggttcg taaaaggcgg gctatagggt aggcttcatc
     3301 tcgccaatct cactgaatcc atcaatttcc acaattcaat taaataccgt caaaccgatg
     3361 ccgtcattcc cgcgcaggcg ggaatctaga ccttgggata
//
LOCUS       DAWFIE010000123         3386 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWFIE010000123 DAWFIE010000000
VERSION     DAWFIE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.124"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            153..605
                     /gene="dut"
                     /locus_tag="WGQ71_001961"
     CDS             153..605
                     /gene="dut"
                     /locus_tag="WGQ71_001961"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ0707905.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            682..1869
                     /gene="dapC"
                     /locus_tag="WGQ71_001962"
     CDS             682..1869
                     /gene="dapC"
                     /locus_tag="WGQ71_001962"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ0707906.1"
                     /translation="MNTLLKQLKPYPFARLHEAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEIFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            2132..2911
                     /gene="yaaA"
                     /locus_tag="WGQ71_001963"
     CDS             2132..2911
                     /gene="yaaA"
                     /locus_tag="WGQ71_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ0707907.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AVEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            2994..3084
                     /locus_tag="WGQ71_001964"
     tRNA            2994..3084
                     /locus_tag="WGQ71_001964"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3028..3030,aa:Ser,seq:tga)
     gene            3162..3251
                     /locus_tag="WGQ71_001965"
     tRNA            3162..3251
                     /locus_tag="WGQ71_001965"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3196..3198,aa:Ser,seq:cga)
BASE COUNT          887 a          797 c          823 g          879 t
ORIGIN      
        1 gtgtgcataa tgttcgtctt cataacggga agggcgggca ttatacactg gcaacggatt
       61 tcaaaacaaa accgatttgc cgtgtttcag cgtaaacacg gcttgtgtat aatctcccat
      121 ctttgaaatc ggccgtatgc aggagcaaga cgatgaatat tgaagtagaa atgaaagtat
      181 tggacgagag gatggcggat tttatccctg cctatgcaac ggagggttct gcaggtttag
      241 atttgcgcgc ctgtttggat gaggaagtcg tcttgcagcc gggtgaaacg tttcttgtac
      301 cgacaggttt ggcaatttat ttggcgaatc ccgcatatgc cgccgttttg ctgccccgtt
      361 ccggcttggg gcataaacac ggcattgttt tgggcaattt ggtcggcttg attgactccg
      421 attatcaagg ggaattgaag gtttcgttat ggaacagaag cagcgagccg tttactgtca
      481 aaccgtttga gcgtatcgcg cagatggtta tcgtgccaat cgtgcaggcg cgtttcaaac
      541 gtgtcgagga atttgtcgga agcagccggg gtgagggcgg cttcggcagt acgggtttgc
      601 attgaatata aaatgccgtc tgatgggtgc cgtcaggttc agacggcata tcttccgata
      661 taccgataac cagagaaaat catgaatacc ttactcaagc agctcaaacc ctatcccttt
      721 gcccgactgc acgaggcgat gcagggcatt tccgcgcctg aaggcatgga agccgtccca
      781 ctgcatatcg gcgaaccgaa acatccgaca ccgaaagtca ttacggatgc gctgaccgcc
      841 tcattgcacg agttggaaaa atatccgctg acggccggtt tgcctgaact gcgtcaggcg
      901 tgtgcgaact ggttaaaacg ccgttatgat gggttgacgc tgaattccga caccgaaatt
      961 ctgccggttt tgggaagtag ggaggcgttg ttttcttttg tccaaaccgt gttgaaccct
     1021 gtttcagacg gcctcaaacc cgtaatcgtc agcccgaatc ccttttatca gatttacgaa
     1081 ggtgcgacac ttttgggcgg cggtgaaatc cattttgcta attgccccgc gccgtctttc
     1141 aaccccgatt ggcgcagtat ttccgaagag gtttggaaac gcaccaaact ggtgttcgtc
     1201 tgctcgccca acaaccccag cggcagcgtg ctggatttgg acggatggaa agaaattttt
     1261 gatttacagg ataaatatgg tttcattatt gcctcggatg aatgctattc cgaaatctat
     1321 ttcgacggca acaaaccttt gggctgcctg caagccgctg cacagttggg tcgaagcagg
     1381 caaaaactgc ttatgttcac cagtttgtcc aagcggtcca atgttccggg cctgcgttcc
     1441 ggttttgtcg ccggcgatgc cgaactgctt aaaaactttc tgctttacag aacctatcac
     1501 ggcagtgcaa tgagcattcc cgtgcagcgc gcaagcattg ccgcttggga tgacgaacag
     1561 cacgttatcg acaaccgccg cctgtatcag gaaaaatttg agcgcgttat tcccattttg
     1621 caacaggtat ttgatgttaa attaccggat gcctcgtttt acatctggtt gaaagtccct
     1681 gatggcgacg atttggcatt tgcacgcaat ttatggcaaa aagccgctat ccaagtattg
     1741 cccggacgtt ttttggcacg ggataccgaa cagggcaatc ccggggaagg ttatgtgcgt
     1801 attgctttgg ttgccgatgt cgcaacttgt gtcaaagctg cggaaaccat tgtttcccta
     1861 tatcggtaaa gaataaaaaa atgccgtctg aacttttgtt cagacggcat ttttcaatat
     1921 tttacggttg aatttgctat aacggtattt atagtggatt aacaaaaatc aggacaaggc
     1981 gacgaagccg aagacagtac agatagtacg gcaaggcgag gcaacgctgt actggtttaa
     2041 atttaatcca ctatacttca cttttaatcg gcttgcccgc aaacacgttt aaacttaaaa
     2101 tccccgtgtt tgacacaata ccgagcagat tatgtttttt gtcctttccc ctgcgaagaa
     2161 ccttaatgaa aaagaccctg cccctgtcag cgagtttacc caacccgacc tgctggcaga
     2221 gtccgacatt ctaatgcagc agttgcgcga gcttgcgccg caacagattg ccgaactgat
     2281 gcacgtttcc gacaaaattg ccctcttaaa cgcgcagcgc aatgcagaat ggaacacgcc
     2341 gtttacgccg gaaaacgcca aacaggcggt ctttatgttc aacggcgatg tttacgaagg
     2401 tatggatgca aacacattgg atattggaca gatacgctat ctgcaaaacc atgtccgcct
     2461 gctgtccggt ctgtacggtc ttcttcgccc gttagacctg atacagccct atcgtttgga
     2521 aatggggacg gcatttgcca atttgcgcgg caagaatttg tatgagtttt ggggcgacat
     2581 cattaccaac cttttaaatg acacgcttgc ccaagcaggc agcaatacgc ttgtcaacct
     2641 tgcctcacag gaatatttca agtccgtcaa cacgaaaaaa cttcgggcgc ggctgattac
     2701 cccgatattt aaagacgaaa aaaacggtaa atataaaatc atcagtttct atgccaagcg
     2761 cgcgcgcgga ttaatggtgc gctatgcggt agaacatcat attaccgacc ctgaaatgct
     2821 gaaaaatttt aattacgaag gctacgcatt caatgacgcg gcttcaaatg aaagcgaatg
     2881 ggtttttatg cgttcggaac aaataaaatg aaaacaataa attaagtatt ttccgaaaaa
     2941 agtgcttggc aaaatgtata aacttcatta ttattcctac tcttcaagaa gacggaagcg
     3001 tggcagagcg gtttaatgca acggtcttga aaaccgtcga gggttgatag ccctccgtga
     3061 gttcgaatct caccgcttcc gccaatcctt gaggtcaaaa accaaataaa aatgaagtag
     3121 cataaagcat cggcattttt atttggtttt acttcattat cggaaacgtg gcagagaggc
     3181 tgaatgcagc ggactcgaaa tccgctgagg gtgcaaatcc tccgtgggtt cgaatcccac
     3241 cgtttccgcc acaaaacaaa accgccctga ttcggggcgg ttttgttttg ttcaagattg
     3301 agacctttgc aaaattcctt tccctcccga cagccgaaac ccaaacacag gttttcagct
     3361 gttttcgccc caaataccgc ctaatt
//
LOCUS       DAWFIE010000124         3310 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWFIE010000124 DAWFIE010000000
VERSION     DAWFIE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.125"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            471..1094
                     /locus_tag="WGQ71_001966"
     CDS             471..1094
                     /locus_tag="WGQ71_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0707908.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGTLLLSPLAQAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1205..2587
                     /locus_tag="WGQ71_001967"
     CDS             1205..2587
                     /locus_tag="WGQ71_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0707909.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYAGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNRDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2590..3114
                     /locus_tag="WGQ71_001968"
     CDS             2590..3114
                     /locus_tag="WGQ71_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ0707910.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          696 a          870 c          989 g          755 t
ORIGIN      
        1 tccgtggagg attaaggggg tatttgggta aaattaggag gtatctgggg cgaaaacagc
       61 tgaaaacctg tgttggggtt tcggctgtcg ggagggaaag gaattttgca aaggcctcaa
      121 gatatagtgg attaaattta aattagtacg gtaagacgag gcaacgctgt actggtttaa
      181 atttaatcta ctatatttcc gcctgctgcc acccgaaaag cgtgatgcgc tgatggtgct
      241 gctgcataat ccggacgacg gataaccgat gaaaacggaa atgcccgggt ggtgttgcct
      301 gtgcctgttt ggacaagcgt tcttgagggc ggtaaaatca aggtttgccc gaaaagggca
      361 gggcgatatg ccgcgcttgg attttgcgcg cggtttttcg cgttggaagc aatcatgtct
      421 gtttgaatat ttaatgccgt ctgaaacgtc cggacgcgcc cccgttccct atgaaaaaac
      481 cgaccgatac cctacccgtc aatctacaac gccgccgcct gttgtgtgct gccggcacgc
      541 tgttgctcag cccgctggca caagccggcg cgcaacgtga agaaacgctt gccgacgatg
      601 tggcttccgt gatgaggagt tctgttggca gcgtcaatcc gccgaggctg gtgtttgaca
      661 atccgaaaga gggcgaacgt tggttgtctg ccatgtcggc acgtttggca aggttcgtcc
      721 ccgaggagga ggagcggcgc aggctgctgg tcaatatcca gtacgaaagc agccgggccg
      781 gtttggatac gcagattgtg ttggggctga ttgaggtgga aagcgcgttc cgccagtatg
      841 caatcagcgg cgtcggtgcg cgcggtctga tgcaagttat gccgttttgg aaaaactaca
      901 tcggcaaacc ggcgcacaac ctgttcgaca tccgcaccaa cctgcgttac ggctgtacca
      961 tcctgcgcca ttaccggaat cttgaaaaag gcaacatcgt ccgcgcgctt gcccgcttta
     1021 acggcagctt gggcagcaat aaatatccga acgccgtttt gggcgcgtgg cgcaaccgct
     1081 ggcagtggcg ttgattttga acccgcgccg caaccgaaat acggcgaatc ctgtataatc
     1141 cgaaaatctg ttcactggaa gttcagacgg cattgcaact gttgatgccg tctgaaaaaa
     1201 tatgatggca agagacaacc gcatccaaat gtttccgcac gaatggcgcg ccagtacgac
     1261 gctttccggc gtgtacgcgc tgcgtatgct gggtatgttc ctcgtgctgc ccgtattggc
     1321 ggtgtatgcc gcctcgctgc ccggcgcgga aggcaacaaa acgctggtcg ggctggcaat
     1381 gggcatttac gggctgacac aggctctgct gcaactgcct ttgggcatcg cttccgacaa
     1441 gttcgggcgc aagaaaacca tttatgcggg actggtcgtg tttgcggcgg gcagctttct
     1501 tgccgccgcc gccgatacgc tgcccatgct ggtcgccgca cgcgccatac agggtgcagg
     1561 ggcggtcagt gcggcggtta ccgcgctgct ggcggatttg acgcgcgacg gcgtacgtac
     1621 ccgcgcaatg gcgatgatcg gtttgagtat cggtttgacg ttttcggtca gcctcgtcgt
     1681 tgcccccgtg attgccgacg cggtcggcgt tcgcggactg tttatgctga ccggcattct
     1741 gaccgtcatc agcatcggcg tggtggcgtg gatgactccc gatcccgaag tttccaagct
     1801 gcacgaagat acgcaggcgc agccttcgcg cataggcgaa gttttgaaaa accgtaggct
     1861 gctgacgctt gatttcggca ttttcgccct gcacgccgca caaatggcat tgtttaccgc
     1921 gctgcctttc gcgatgaccc agctcggttt ggaaaaaata cagcattgga aagtctatct
     1981 gccttcgacc attacgggct tggtggtgat ggttccgctg attatcgtcg gcgagacgcg
     2041 caacaagctt aagcaggttt ttgttttggg tatcgtctgt attgcggcgg cgcagctcgg
     2101 tttgctgtcc ggtatgcgct cggtaggctt gattaccgct tatttggttg tttactttat
     2161 cggttttaat gtgttggaag cgagcctgcc gtctatggtt tccaaaatcg cgccgtccga
     2221 cctgaagggt acggcgatgg gcgtgtacaa cacgatgcag tcgctcggac tgtttgccgg
     2281 cggcgcggca ggcggtttgc tgtttcaaaa atacggcttt tccggcgtgt ttgccttttg
     2341 cagtatattg atgctgctgt ggctggtaat tgccgtttta tcgcctgcgc ccaagcccgt
     2401 caaaaacctc agttaccctg tcggcggcgt gtggcagggc aatcgggacg gattgcaacg
     2461 cgccctgttg caactcgaag gcgtggaaga catcggtttc agtttcgacg ggcagaccgt
     2521 ctatctcaaa gtgttgcaga agggtttcga tcaggctgcc gctgaaaaaa tcattacagg
     2581 agtttaaaaa tgtcattgaa caaagttatc cttatcggcc gcctcggacg cgatcccgaa
     2641 gtgcgctata tgcccaacgg cgaggcggtg tgtaatttca gcgtcgccac cagcgaaact
     2701 tggaacgacc gcaacggcca acgtgtagag cgtactgagt ggcacaacat cacgatgtac
     2761 cgcaaactgg cggaaattgc cgggcaatac ctcaaaaaag gcgggctggt ttatctggaa
     2821 ggcagaatcc aaagccgcaa ataccaaggc aaagacggca tcgaacgcac cgcttacgat
     2881 attgtcgcca acgaaatgaa aatgttgggc gggcgcaatg aaaacagcgg cggtgcgcct
     2941 tacgaggaag gttacggtca gagtcaggag gcttaccaac gccccgcaca gcaaagccgg
     3001 cagcccgcct ccgacgcgcc gtcccatccc caagaagcac cagccgcgcc gcgccgccaa
     3061 cccgtgcctg ccgccgcccc ggtcgaggat attgacgacg acatcccgtt ctgaatttta
     3121 cggccggaca tcctcgcaga gggaaatcat aaaggacgga gaaaccttaa accttacggg
     3181 gcagggtttc tcttttttgc atccgagccg gcttgttgcc gccccttgcg ttgggcggaa
     3241 cgttgcgatt gcgccgttcg ggcgttttga ggcagtgatg cctgccgcac gtccccgctg
     3301 ttttcaggcg
//
LOCUS       DAWFIE010000125         3250 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWFIE010000125 DAWFIE010000000
VERSION     DAWFIE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.126"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            115..2880
                     /locus_tag="WGQ71_001969"
     CDS             115..2880
                     /locus_tag="WGQ71_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0707911.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGALKFNSDSGKWEP
                     DLQKQQWKYNPYKKYNNQELQKYIEEHDKSWRENLAPQYDITPIDPSSLKQQSAGNLF
                     KLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYANLNLTAAYNSGRQK
                     YPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKNR
                     FPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALKK
                     DIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYKKHCNRSCGIYEPVLKK
                     YGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPERA
                     NTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVNSTG
                     LAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNAS
                     KEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYID
                     GTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRYI
                     DPLDAGNDAATQRYYSSFDPKDKDEEVTCNADKTLCNGKYGGTSKSVLTNFARGRTFL
                     MTMSYKF"
     gene            2910..3152
                     /locus_tag="WGQ71_001970"
     CDS             2910..3152
                     /locus_tag="WGQ71_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707912.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPRRRESRHSMLRQFIGND"
BASE COUNT          921 a          801 c          788 g          740 t
ORIGIN      
        1 atccaattga aatcttcaga cggtatatcg aatttacact ttttaatgtt tatgccgcct
       61 aaaaaaatgc taatatattt cttaattgtc tgactgttta ttgttgagga aaatatgaga
      121 tcttctttcc ggttgaagcc gatttgtttt tatcttatgg gtgttatgct atatcatcat
      181 agttatgccg aagatgcagg gcgcgcgggc agcgaggcgc agatacaggt tttggaagat
      241 gtgcacgtca aggcgaagcg cgtaccgaaa gacaaaaaag tgtttaccga tgcgcgtgcc
      301 gtatcgaccc gtcaggatat attcaaatcc agcgaaaacc tcgacaacat cgtacgcagc
      361 atacccggtg cgtttacaca gcaagataaa agctcgggca ttgtgtcttt gaatattcgc
      421 ggcgacagcg ggttcgggcg ggtcaatacg atggtggacg gcatcacgca gaccttttat
      481 tcgacttcta ccgatgcggg cagggcaggc ggttcatctc aattcggtgc atctgtcgac
      541 agtaatttta ttgccggact ggatgtcgtc aaaggcagct tcagcggctc ggcaggcatc
      601 aacagccttg ccggttcggc gaatctgcgg actttaggcg tggatgacgt cgttcagggc
      661 aataatacct acggcctgct gctaaaaggt ctgaccggca ccaattcaac caaaggtaat
      721 gcgatggcgg cgataggtgc gcgcaaatgg ctggaaagcg gagcatctgt cggtgtgctt
      781 tacgggcaca gcaggcgcag cgtggcgcaa aattaccgcg tgggcggcgg cgggcagcac
      841 atcggaaatt ttggcgcgga atatctggaa cggcgcaagc agcgatattt tgtacaagag
      901 ggtgctttga aattcaattc cgacagcgga aaatgggagc cggatttaca aaagcaacag
      961 tggaaataca atccgtataa aaaatacaac aaccaagaac tacaaaaata catcgaagag
     1021 catgacaaaa gctggcggga aaacctggcg ccgcaatacg acatcacccc catcgatccg
     1081 tccagcctga agcagcagtc ggcaggtaat ctgtttaaat tggaatacga cggcgtattc
     1141 aataaataca cggcgcaatt tcgcgattta aacaccaaaa tcggcagccg caaaatcatc
     1201 aaccgcaatt atcaattcaa ttacggttta tctttaaact catatgccaa cctcaatctg
     1261 accgcagcct acaattcggg caggcagaaa tatccgaaag ggtcgaagtt tacaggctgg
     1321 gggcttttaa aggattttga aacctacaac aacgcaaaaa tcctcgacct caacaacacc
     1381 gccaccttcc ggctgccccg tgaaaccgag ttgcaaacca ctttgggctt caattatttc
     1441 cacaacgaat acggcaaaaa ccgctttcct gaagaattgg ggctgttttt cgacggtccg
     1501 gatcaggaca acgggcttta ttcctatttg gggcggttta agggcgataa agggctgctg
     1561 ccccaaaaat caaccattgt ccaaccggcc ggcagccaat atttcaacac gttctacttc
     1621 gatgccgcgc tcaaaaaaga catttaccgc ttaaactaca gcaccaatac cgtcggctac
     1681 cgtttcggcg gcgaatatac gggctattac agctcggaag gcgaatttaa gcgggcattc
     1741 ggagaaaact cgccgacata caagaaacat tgcaaccgga gctgcgggat ttatgaaccc
     1801 gtattgaaaa aatacggcaa aaagcgcgcc aacaaccatt cggtcagcat tagtgcggac
     1861 ttcggcgatt atttcatgcc gttcgccagc tattcgcgca cacaccgtat gcccaacatc
     1921 caagaaatgt atttttccca aatcggcgac tccggcgttc acaccgcctt aaaaccagag
     1981 cgcgcaaaca cttggcaatt tggcttcaat acctataaaa aaggattgtt aaaacaagat
     2041 gatacattag gattaaaact ggtcggctac cgcagccgca tcgacaacta catccacaac
     2101 gtttacggga aatggtggga tttgaacggg aatattccga gctgggtcaa cagcaccggg
     2161 cttgcctaca ccatccaaca ccgcaatttc aaagacaaag tgcacaaaca cggttttgag
     2221 ttggagctga attacgatta tgggcgtttt ttcaccaacc tttcttacgc ctatcaaaaa
     2281 agcacgcaac cgaccaactt cagcgatgcg agcgaatcgc ccaacaatgc gtccaaagaa
     2341 gaccaactca aacaaggtta tgggttgagc agggtttccg ccctgccgcg agattacgga
     2401 cgtttggaag tcggtacgcg ctggttgggc aacaaactga ctttgggcgg cgcgatgcgc
     2461 tatttcggca agagcatccg cgcgacggca gaagaacgct atatcgacgg caccaacggg
     2521 ggaaatacca gcaatgtccg gcaactgggc aagcgttcca tcaaacaaac cgaaaccctt
     2581 gcccgccagc ctttgatttt tgatttttac gccgcttacg agccgaagaa aaaccttatt
     2641 ttccgcgccg aagtcaaaaa tctgttcgac aggcgttata tcgatccgct cgatgcgggc
     2701 aatgatgcgg caacgcagcg ttattacagt tcgttcgacc cgaaagacaa ggacgaagaa
     2761 gtaacgtgta atgctgataa aacgttgtgc aacggcaaat acggcggcac aagcaaaagc
     2821 gtattgacca attttgcacg cggacgcacc tttttgatga cgatgagcta caagttttaa
     2881 aggcagcccg cgttttgtag aaaaccgcaa tgccgtctga aagcccttca gacggcattt
     2941 gtttccccaa acgcatcatc ctgccgcaag cctatgccaa tccgttttat cgcatcggca
     3001 actcaaagaa aaatccattt cattcccacg cagggaagcc ggtttttgat ttcggttatt
     3061 tttggttgtt tcgggtaatt tatgagtcgt cattcccgcg caggcgggaa tctagacatt
     3121 caatgctaag gcaatttatc ggaaatgact gaaactcaaa aaactggatt cccactttcg
     3181 tgggaatgac gcggtgcagg tttccgtacg gatggattcg tcattcccgc gcaggcggga
     3241 atctagtccg
//
LOCUS       DAWFIE010000126         3175 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWFIE010000126 DAWFIE010000000
VERSION     DAWFIE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.127"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            52..1005
                     /locus_tag="WGQ71_001971"
     CDS             52..1005
                     /locus_tag="WGQ71_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ0707913.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSICGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1116..1439)
                     /locus_tag="WGQ71_001972"
     CDS             complement(1116..1439)
                     /locus_tag="WGQ71_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707914.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1612..2973)
                     /locus_tag="WGQ71_001973"
     CDS             complement(1612..2973)
                     /locus_tag="WGQ71_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ0707915.1"
                     /translation="MLKKWLNKMLPSGQSSKKAESKTIIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSSKLGFDLSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLATLMWPELERHWKSNLQQGLKPVPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
BASE COUNT          746 a          932 c          767 g          730 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatctg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgca
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggc ttcctgcccg gagacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt tgaccgtgta
      661 accaagctga ttgaaaaagg cctgattgag attgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 aacgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcaggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tttctttatc cgtccaaccg atacatcaat cgaattttct gcatcggatt
     1141 gcctcatctt cgcaaaacat tgccgaagtt tcgcggttgc acgcctgacg cactttctgc
     1201 cgcgccacgc cttcattccg atcgcttgcc tcaaacgtct tctgaaaagg cgtaacgctg
     1261 catacataca tggtggaatt tgaaccgaaa aggtcgattt tgacggtatt gccgccgctt
     1321 tccaatacgg cgacacggtg ttccaactgc gccagacgtg cttccaaatc gccgattctg
     1381 tcggcaaatg cgggcatggc ggcacacgcg gtcaaaatgc ccaaaataca ttttttcatc
     1441 aatttttcct tgttgcttga accctgccca cccggacatc ctttttccga gaggcagggt
     1501 cagactttat ttacggagat gaggcccctt acaaatcagg ataatacata gaacggcact
     1561 ttatgcttca ccccatgtgt taaaacttca ttgctccttg tctgttgacc cttattccca
     1621 atccgttccc acaaccttcg gcttgggttt gcgcctgcga cggcggcgtt ttttcgcctg
     1681 tccttcgttt ttttcgtgtc gggcggcggc ttcgtttttg gtcatctccg accgctgttc
     1741 cgtcgatgcc gtctgaaacg ccgtccacca ctcggcaagg gcgcggtccg cattgccggt
     1801 ttcggcgcgc aagagcagga aatcataggc ggcacggaaa cgcgcctgtg caaacagttt
     1861 gtgcggcctt gcgcctttgc ggttttcaaa ctgcggctgg aacatccaaa tttcgcgcat
     1921 cgtggcggaa aagcgttgcg gtacgcccca accgcgttcg acggtttcgc gcatcgtatt
     1981 gattgcatcg gacagggcgg gcacgggttt caaaccctgt tgcagattgc ttttccaatg
     2041 gcgttccaac tcgggccaca tcagggtcgc cagtacgaaa ccgaccgaaa ccgatttgtc
     2101 ggcacgcagc cgctcatcgg tatttttcag ggcaagcacc gtcatttttc cggcgatgcc
     2161 gtctgaaacg cgcaaggcat tgagcagcgg atggatgtcg tccggtatgt caaatccgtt
     2221 caaacgtttc agacactcgc gcgcgtgccc tgaaaacagc agtttcataa tttcgtcgaa
     2281 cagccgcgct accggttcgt gcttcagacg gcatatcgat tcggcaatcg gtgcggcggt
     2341 ttcttccgac agatcaaagc ccaatttgct cgacaggcgg atggcgcgca aaatcctgac
     2401 agggtcttcc tgatagcgtt cggcggcatc accaatcata accagcctgc gggcggcaac
     2461 atcggcaatc ccgttgtgga aatccaaaat ctcttctttt tcaggatcgt aatacaaggc
     2521 attgcaggta aaatcgcgcc gcatcgcatc ttcttcgatg ctgccgtagg tgttgtcctt
     2581 catgattctg ccgtgcgcgt tctgatgaac cttcgcaccg ccgcgaaacg tcgttacttc
     2641 gataatctcc gcaccgttca tcacatggac aatctgaaaa cgcctgccga tgatgcggct
     2701 gcggcggaag agtttgtgca cctgttcggg cgtggcatcg gttgcgacat cgaaatcctt
     2761 gggttcgatg ccgagcagca ggtccctgac cgcgccgccg accacataag cctgaaaccc
     2821 cgcccctttc aggcggcgta tgacgttttc ggcggcaaag ctcaacattt cggcacggat
     2881 gttgtgtctt tcggcaggaa tgatcgtttt actttccgct tttttactgc tctgaccgga
     2941 aggcagcatc ttattcaacc attttttcag cataaatatt ctaacccgaa gagcggcatc
     3001 tccacgggca accgtgttca gacggcatca ggcggtacga ttccgcaaga tgccgtaacc
     3061 ataaacaaaa ggcgtagaaa ctacgccgtc cgaatcacgc cgccctcgcg gcggcggcaa
     3121 cgcgccatta taacagattg ccctttaaaa aaatcagacc ctgcttttgt ggcgg
//
LOCUS       DAWFIE010000127         3027 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWFIE010000127 DAWFIE010000000
VERSION     DAWFIE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.128"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..748
                     /gene="mafB"
                     /locus_tag="WGQ71_001974"
     CDS             <1..748
                     /gene="mafB"
                     /locus_tag="WGQ71_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ0707916.1"
                     /translation="DPNKNWWANRMDDIRGIVQGAVNPFLMGFQGVGIGAITDSAVSP
                     VTDTAAQQTLQGINHLGNLSPEAQLAAATALQDSAFAVKDGINSARQWADAHPNITAT
                     AQTALSAAEAAGTVWRGKKVELNPTKWDWVKNTGYKTPAARPMQTLDGEMAGGNKPVV
                     KSIRPTTRDELRQALQEQGFRRTGSDAAQYETWKGPDGVKIDIRPNGEVIRTQRVPRT
                     DGVQGKYPQRQDYEGNPLPNNHHHSGYFVK"
     gene            745..1062
                     /locus_tag="WGQ71_001975"
     CDS             745..1062
                     /locus_tag="WGQ71_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707917.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            1132..1800
                     /gene="mafB"
                     /locus_tag="WGQ71_001976"
                     /pseudo
     CDS             1132..1800
                     /gene="mafB"
                     /locus_tag="WGQ71_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            1810..2016
                     /locus_tag="WGQ71_001977"
     CDS             1810..2016
                     /locus_tag="WGQ71_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707918.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            2149..2400
                     /locus_tag="WGQ71_001978"
     CDS             2149..2400
                     /locus_tag="WGQ71_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707919.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            2397..2768
                     /locus_tag="WGQ71_001979"
     CDS             2397..2768
                     /locus_tag="WGQ71_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0707920.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            2854..2979
                     /locus_tag="WGQ71_001980"
                     /pseudo
     CDS             2854..2979
                     /locus_tag="WGQ71_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
BASE COUNT         1055 a          544 c          633 g          795 t
ORIGIN      
        1 cgaccccaat aaaaattggt gggctaaccg tatggatgat attcgcggca tcgtccaagg
       61 tgcggttaat ccttttttaa tgggttttca aggagtaggg attggggcaa ttacagacag
      121 tgcagtaagc ccagtcacag atacagccgc gcagcagact ctacaaggta ttaatcattt
      181 aggaaattta agtcccgaag cacaacttgc ggccgcaacc gcattacaag acagtgcttt
      241 tgcggtaaaa gacggtatca actctgccag acaatgggct gatgcccatc cgaatataac
      301 tgcaacagcc caaactgccc tttccgcagc agaggccgca ggtacggttt ggagaggtaa
      361 aaaagtagaa cttaacccga ctaaatggga ttgggttaaa aataccggct ataaaacacc
      421 tgctgcccga cctatgcaga ctttagatgg agagatggca ggtggaaaca agccagttgt
      481 taaatctatc agaccaacta cgcgagatga attacgtcaa gcattgcaag aacaaggttt
      541 tagacgtact ggttcagatg cggctcaata tgaaacatgg aaaggtcctg atggcgtgaa
      601 aatagatatt cgtccaaatg gagaggttat aagaacccaa agagtgccgc gaaccgatgg
      661 tgtacaggga aaatatccgc aacgacaaga ttatgaaggc aatccattgc caaataatca
      721 tcatcattct ggatattttg tcaaatgaaa aaaaatattt ttcacaatgt aagcctttat
      781 gaaataatct tttccgataa tggaaatacc cttacattat cttttacaga tacaattgaa
      841 ggtaattatt tcggatatat caaatgcagt aatattttga attttaaatt agatacaaat
      901 aatttcgtag attatgagga taaggaagat agcttgtttc ccttgtttat acccgaaata
      961 gagctatata aataccaatt ttatagtgaa attattattg atgtagggat tattataaaa
     1021 atatctgctg aaacaattaa ttttgagcca ctgggaaaat agtaactgct ttctcagcag
     1081 ccgtagcaac tgtattttta cccgacgggg taaaaataca gttgctacat ctctgcgcag
     1141 cagactctac aaggtattaa taatttagga aatttaagtc cggaaacaca acttgctgcc
     1201 gcgagcctat tacaggatag tgcttttgcg gtaaaagacg gcattaattc cgccagacaa
     1261 tgggctgatg cccatccgaa tataactgca acagcccaaa ctgctcttgc cgcagcagag
     1321 gccgcaacta cggtttgggg cggtaaaaaa gtagaactta acccgactaa atgggattgg
     1381 gttaaaaata ccgattataa aaaacctgct acccgccata tgcagactgt agatggggag
     1441 atggcagggg ggaatagacc gcctaaatct ataacgtcca acagcaaagc agatgcttcc
     1501 acacaaccgt ctttacaagc gcaactaatt ggagaacaaa ttagtagtgg gcatgcttat
     1561 aacaagcatg tcataagaca acaagaattt acggatttaa atatcaattc accagcagat
     1621 tttgctcggc atattgaaaa tattgttagc catccaacaa atatgaaaga gttacctcgc
     1681 ggtagaactg cgtattggga tgataaaaca gggacaatag ttatccgaga taaaaattct
     1741 gacgatggag gtacagcatt tagaccaaca tcaggtaaaa aatattatga tgatttatag
     1801 gaaaaagcca tgaatatact atccataaat aatcaaaact caactatttc actaactcaa
     1861 gatgaagttt ttgttttacg agctatcttg aatgagatat atgcgggcgt atgtgtagat
     1921 tcaagagaat ttgaaaatgt atctggtgtt agaaaacatg aagtagataa tttacaacaa
     1981 cagtttgctg gaatttataa aaaaatgaca acttaacaac ccaaattttt gtcagagcct
     2041 agtgcaaatt acaactatga ttctcttgta gctgcaatga acgaaaaaaa atcatgggtt
     2101 ggcgacaggg ttgatgttgt taatatgcct gatggagcac ctactagtat ggataacacg
     2161 cgtattatgg cagcacgtga agcaggggta aaagtggaag cgaatgttca taattttaat
     2221 gaccgattat catcaaaaga gagaatcagg tttaagcatg atggtattga gcctcaaact
     2281 tggggagaag ctatccagct acgaattaga aagcaagaaa cacaaaaagg agttccagaa
     2341 gggtggagca aaagatttcc taacggaagt atttatgatg taaaggtact taggaaatga
     2401 taaaacaaaa tagttttgtt ccgtatcctg aagcaatgct tcctaaagga tttaaatatc
     2461 cgcaaagtta tttaaaatta gctcaatcca ctcatgccat taactacgat gaacaatatt
     2521 cttttccttg gtggtttgaa aatgcagaaa gcaatatatc agaagtaatt gacatttatt
     2581 ttgaaataac tggcattcca aacctattac cttttgctag aaaccaagag tgggctgcct
     2641 gttttgatat ttcagataaa tcaggtaatc ctaaaattat agtagttaat ttagataata
     2701 caaaatatta cgagactttt gaaaattttg atacttggct aaaagaagct gaaaatgatg
     2761 gttggtagca gccgtaggtc ggattctcga atccgacatt ttccaacagc ggcatttcgg
     2821 aaacgataga cgcgtcaaat atttttgtcg gatacaagta tccggccata cggcaatcat
     2881 acgggtaact ccgccccatc cgtagagact gataacagtc atgaggggta tggatacagc
     2941 gatgaagtag tgcgacaaca tagacaaggg caaccttgat tcacactacc ataaccgctt
     3001 gctaccaagg aaaacaaaat gaaattg
//
LOCUS       DAWFIE010000128         2992 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWFIE010000128 DAWFIE010000000
VERSION     DAWFIE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.129"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(459..1538)
                     /gene="aroB"
                     /locus_tag="WGQ71_001981"
     CDS             complement(459..1538)
                     /gene="aroB"
                     /locus_tag="WGQ71_001981"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ0707921.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMTGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGTLGDISFFEWLEQHMPELMALERAPLIQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAVAAGCVLAA
                     RLSEQLGKISAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSSGVMRFIGL
                     NRLGEAVITEITDTDILRRTLQPYL"
     gene            complement(1618..2130)
                     /locus_tag="WGQ71_001982"
     CDS             complement(1618..2130)
                     /locus_tag="WGQ71_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ0707922.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
BASE COUNT          666 a          711 c          876 g          739 t
ORIGIN      
        1 cgaggtttta agttgctgtt tcgggtttct gttttttgtg gaaataacga gattctaggg
       61 ttgcagaaac ttctccgaaa caacaaaaac ctctccgccg tcattcccac gaaagtggga
      121 atctagaaat gaaaagcaac gggaatttat cggaaacgac cgaaaccgaa cggactggat
      181 tcccgctttt gcgggaatga cgaggtttta agttgctgtt ttgggtttct gttttttgtg
      241 gaaataacga gattctaggg ttgcagaaac ttctccgaaa caacaaaaac ctttccaccg
      301 tcattcctgc gaaagtggga atctagaaat gaaaagcaac gggaacttat cggaaacgac
      361 cgaaaccgaa cggaccggat tcccgctttt acggagaaag atgatggaaa atcatcatcg
      421 tgcgtcaaac ccgcgcggca gcacatcggc agaggaaatc agagatacgg ttgcagggtg
      481 cggcggagaa tgtccgtgtc ggtaatttcg gtgatgacgg cttcgcccag acggttcaac
      541 ccgataaagc gcatcacgcc gctactgact tttttatcgt ggctcatgtg ttccagccat
      601 ttttcaaagg caaacacggg tggcgcggac ggcagtccgg cggcttcgag cagggcggcg
      661 agccgcgcgg tatctgcggc ggagattttg cccagttgtt cggacaaacg cgccgccaac
      721 acgcagccgg cggcgacggc ctcgccgtgc agccaagtac cgtaacccat ctcggtttca
      781 atggcgtgtc cgaaggtgtg tccgaggttg agccatgcgc gtatgccctg ttcggtttcg
      841 tcttgggcga cgatgtctgc cttcatttgg caacagcggt acacggcttg gatgaggggc
      901 gcgcgttcga gtgccatcag ttcgggcata tgctgttcca gccattcaaa aaaactaatg
      961 tcgccgagcg tgccgtattt gatgacttcc gccataccgg cggacagttc gcgggcgggc
     1021 agggtgtgca gcgtgtccaa gtcggcaagc accgcctgcg gctggtagaa cgcgccgatc
     1081 atatttttgc cgagcgggtg gttgatggcg gttttgccgc cgacggagga atcgacctga
     1141 cttaataaag tagtgggaat ttgaacaaat ggcgcgccgc gttgataagt agccgccgca
     1201 aaacccgtca tatcgccgat cacgccgccg cccagtgcga tgagcgtggt tttgcgttcg
     1261 gcgcggtttt gcatcagccc gtcaaagatg aggttgagcg tctgccagtt tttgtgcgcc
     1321 tcgccgtcgg gcaggatgat gctgaaatgg gatacgcctg ccgcatccaa tgccgtctga
     1381 agcgtgccga ggtagagcgg ggcgacggtt tcgttggtga tgatggcggc gcgtttgccc
     1441 aaatgcggtt tgagcaggct tcctgcctgc ggcagcagtc cgttgccgat aaagatgggg
     1501 tagctgtggg acggggtgtg tacggtcagt gttttcattg ttgttcctta aagtttgaac
     1561 cgccggcccg ccgggcgggg cgcggtttgg ttttctgggc gcggcgcata tgccggttta
     1621 tcgggataag cgtttgagca gggtttgcac ggtttcccgg cagtttgccg attctacggt
     1681 aaagtcggcg gtttggcggt aaacggggtc gcgtgcggcg tagagttcac gtaatttcgc
     1741 caaaggatcg gcaacttgca gcaaaggacg gctgttgtcg cagcgcgtgc gttcgagcag
     1801 ggtttcgggc ggggcgtgca gatagacgac cgtgccgctt ttgcggataa gggcgcggtt
     1861 ttcttctttt aacaccgcgc cgccgccggt ggacaggacg atatggggca ggataaccag
     1921 ctttttgagt atggcggttt cgcgcgaacg gaatccctgt tcgccttcca tttcaaatat
     1981 ggtggggatg ggaacgccgg cggctgcggc gatttcgtga tcgctgtcgt aaaaacggta
     2041 atccagccgc tgcgccattt gccggcccag cgtggttttg cccgcgccca tcagtccgat
     2101 gaggatgagt ttgccgttaa agtttttcat cacaattcct taatgtttga caccccgcct
     2161 ttcggggcga cagggttcgg gcttgtcggg ttacggcgga attttatacg aaatcggcag
     2221 ggcggcggta cgtttggaaa aataacccga ccatcccgaa cctttctgat tttaaggaca
     2281 aataaaagaa atcagggagg ttttttattt caggctgtgt tttgacaatc cgttgatttc
     2341 acttatttgt caggaaaagg caattatctt tgcttaggta aacaattatc caattgaata
     2401 tattgaagat aatatgttta tcaatactat agtggattaa caaaaaccag tacagcgttg
     2461 cctcgccttg gctcaaagag aacgattctc taaggtgctg aaacaccaag tgaatcggtt
     2521 ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta
     2581 taaagaccgt tgggcatctg tcgccgtcat tcccgcgcag gcggaaatcc gaacacgtcc
     2641 gcacggaaac ccatatcccg tcattcccac gaaagtggga atctagaacg cagggttgga
     2701 gaaaccgttt tatccgataa gttcctgaac cgacagacct agattcccgc ctgcgcggga
     2761 atgacggggt tttaagttgc cgttttgggt tgctgttttt tgtggaaata acgagattct
     2821 agggttgcag aaacttctcc gaaacaacaa aaacctctcc gccgtcattc ccacgaaagt
     2881 gggaatctag aaatgaaaag cagcaggaat ttatcggaaa cgaccgaaac tgaacggacc
     2941 ggattcccgc ttttgcggga atgacgaggt tttaagttgc tgtttcgggt tt
//
LOCUS       DAWFIE010000129         2706 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWFIE010000129 DAWFIE010000000
VERSION     DAWFIE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.130"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            157..381
                     /locus_tag="WGQ71_001983"
                     /pseudo
     CDS             157..381
                     /locus_tag="WGQ71_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            919..1386
                     /locus_tag="WGQ71_001984"
     CDS             919..1386
                     /locus_tag="WGQ71_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0707923.1"
                     /translation="MACDAGVASSSTIKGKYVAGVKVEKGVITAEMKSSGVNKEIQGK
                     KLSLWAKRQNGSVKWFCGQPVTRADNAKDDAKDDAVTAAATGTDKIDTKHLPSTCRDA
                     SSASRTKTPGFAFKYFQKISRYRVLPESRQMTKKLRHSRAGGNLGLSAQKLIG"
     gene            1432..1731
                     /locus_tag="WGQ71_001985"
     CDS             1432..1731
                     /locus_tag="WGQ71_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0707924.1"
                     /translation="MTGFNDAAGVATSTDIKGKYVQSVEVKNGVVTATMASSNVNNEI
                     KGKKLSLWAKRQNGSVKWFCGQPVTRANTATDADVTAANGTDKKIDTKLEKPFSR"
     gene            2560..>2706
                     /gene="pilE"
                     /locus_tag="WGQ71_001986"
     CDS             2560..>2706
                     /gene="pilE"
                     /locus_tag="WGQ71_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial major subunit PilE"
                     /protein_id="HEZ0707925.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLAEG"
BASE COUNT          861 a          727 c          545 g          573 t
ORIGIN      
        1 tttgcacaaa acacccaagt attgcccgaa tcacggcgaa tggccgaaaa acttcgtcat
       61 tcccgcgcag gcgggaatct aggtttgtcg gcgcagaaac ttatcgggta aaaaggtttc
      121 tccggtcctg agtcctagat tcccactttc gtgggaatga cgggatttaa tgatgccgcc
      181 ggtgtggcat cctccgccga catcaaaggc aaatatgttc agaaagttga agtcaacaac
      241 ggcgtcgtta ccgccacaat gctttcaagc ggcgtaaaca aagaaatcca aggcaaaaaa
      301 ctctccctgt gggccaagcg tcaagacggt tcggtaaaat ggttctgcgg acagccggtt
      361 acgcgcgacg ccgccaccga cgacgacgac accgacaaga aaatcgacac caaactagag
      421 aaaccttttt acccgataag tttccgtacc gacgaggctg gattcccgcc tgcgcgggaa
      481 tgacggcgga gcggtttctg ttttttccga taaattcctc aaacttaaaa tttcatcatt
      541 cccacaagaa cagaaaacca aaaacagaaa cctaaaattt catcattccc tcaaaaacag
      601 aaaacccaaa acagaaacct aaaattcgtc attccctcaa aaacagaaaa cccaaaacag
      661 aaacctaaaa ttcgtcattc ccgcaaaagc gggaatctag ttcgttgagt ttcagttatt
      721 tagaataaat tttgaaactt taatcccgtc attcccacga aagtgggaat ctaggacgta
      781 aaatctaaag aaaccgtttt cccgataaat ttccgtaccg acagacctag attcccgcct
      841 gcgcgggaat gacgaagcta tccttttggc tgaaagtcaa aaatcagccg tcaccgagta
      901 ttacctgaat cacggcgaat ggcctgtgat gccggcgtgg catcttcttc aacaatcaaa
      961 ggcaaatatg ttgcaggggt taaagttgaa aaaggcgtca ttaccgcaga aatgaaatca
     1021 agcggcgtaa acaaagaaat ccaaggcaaa aaactctccc tgtgggccaa gcgtcaaaac
     1081 ggttcggtaa aatggttctg cggacagccg gttacgcgcg ccgacaacgc caaagacgac
     1141 gccaaagacg acgccgttac cgccgccgcc accggcaccg acaaaatcga caccaagcac
     1201 ctgccgtcaa cctgccgcga cgcttcatct gccagccgta caaaaacacc cggtttcgct
     1261 ttcaaatact tccaaaaaat cagccgttac cgagtattgc ccgaatcacg gcaaatgact
     1321 aaaaaacttc gtcattcccg cgcaggcggg aatctaggtt tgtcggcgca gaaacttatc
     1381 gggtaaaaag gtttctccgg tcctgagttc tagattccca ctttcgtggg aatgacggga
     1441 ttcaatgatg ccgccggcgt ggcaacctcc accgacatta aaggcaaata tgttcaaagc
     1501 gttgaagtca aaaacggcgt cgttaccgcc acaatggctt caagcaacgt aaacaacgaa
     1561 atcaaaggca aaaaactctc cctgtgggcc aagcgtcaaa acggttcggt aaaatggttc
     1621 tgcggacagc cggttacgcg cgccaacacc gccaccgacg ccgacgtcac cgccgccaac
     1681 ggcaccgaca agaaaatcga caccaaacta gagaaaccgt tttcccgata agtttccgta
     1741 ccgacaaggc tggattcccg cctgcgcggg aatgacggcg gagcggtttc tgttttttcc
     1801 gataaattcc taaaactcaa aatttcatca ttcctacaaa aacagaaaac caaaaacaga
     1861 aacctaaaat tcgtcattcc cgcaaaagcg ggaatctagt tcgttgagtt tcagttattt
     1921 agaataaatt ttgaaacttt aatcccgtca ttcccacgaa agtgggaatc caggacgcaa
     1981 aatctcaaga aaccgttttc ccgataagtt tctgcaccga caaggctgga ttcccgcctg
     2041 cgcgggaatg acgaagccat ccgcacggaa acctgccgcg acgattcaac tgccgtttgc
     2101 acaggaacac cacgcgccga tttcaaacac tttccaagaa aacggaaatt tttaaaaatt
     2161 aaatttaaaa tttcccaccc aacccaccct atgctaccgc gcaaattcaa aaatctcaaa
     2221 ttccgaccca atcaacacac ccgatgcccc atgccaatag agatacccca cgccaataaa
     2281 aaagtgccga aaatcggcac taaaactgac aattttcggc actgctgaca aaaaacggta
     2341 accccctgct tcctcaaacc acgcccaaac gaaagaaaat gtaaaataaa aacaacaata
     2401 taaagataaa cgcataaatt ttcacctcaa aacacaaact tggtaccaat cttgctttat
     2461 aatacacagg tatcgcaaca aaataccgat ggtcaaatac attgcataat gccgatggcg
     2521 taagcttgaa gcatttcctt tccaattagg agtaatttta tgaacaccct tcaaaaaggt
     2581 tttaccctta tcgagctgat gattgtgatt gccatcgtcg gcattttggc ggcagtcgcc
     2641 cttcctgctt atcaagacta cacagcccgc gcacaagttt ccgaagccat ccttttggct
     2701 gaaggt
//
LOCUS       DAWFIE010000130         2699 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWFIE010000130 DAWFIE010000000
VERSION     DAWFIE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.131"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            251..583
                     /locus_tag="WGQ71_001987"
                     /pseudo
     CDS             251..583
                     /locus_tag="WGQ71_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
     gene            complement(799..1593)
                     /locus_tag="WGQ71_001988"
     CDS             complement(799..1593)
                     /locus_tag="WGQ71_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ0707926.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGCAELGAIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVEWIRENQ"
     gene            1637..1741
                     /locus_tag="WGQ71_001989"
                     /pseudo
     CDS             1637..1741
                     /locus_tag="WGQ71_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1802..2527
                     /locus_tag="WGQ71_001990"
     CDS             1802..2527
                     /locus_tag="WGQ71_001990"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ0707927.1"
                     /translation="MPRFHLPENLSVGQTVNLPDNIVRHLNVLRVRPNENITLFNGKG
                     KAHAAQLTVLEKRRAEAEILREETADTESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAAIAAMQTLWGDFA"
BASE COUNT          715 a          842 c          601 g          541 t
ORIGIN      
        1 ggctatgccg gctgctgccg cccattgcac cttcgccgaa tcctgccgcc caccgccgaa
       61 gccctcatgc gttcgcggta cagcgcgtat gtgctactat agtggattaa caaaaaccag
      121 tacagcgttg cctcgcctta gctcaaagag aacgattctc taaggtgctg aagcaccgag
      181 tgaatcggtt ccgtactatt tgtactgtct tcggcttcgt cgccttgtcc tgatttttgt
      241 taatccacta tacatcatcg ctactaccgt tccggcgcaa caaacattcc tcgatgccgc
      301 cgaactgatg caatggagta tagaaaccga agggctgggc ttgaacgtca tctcgcacaa
      361 gatactcggc aaagaccacg cccaagtcga atttgaagcc tacttccgag acggacaaca
      421 ccgatccgcg catcacgaac tgtccggctt catcaacatc ggcggacaat ggtattttat
      481 cgatcccacc gttccgcatc ctgcgatgaa acaaccctgc atttgcggat cgggcaaaaa
      541 attcaaagcc tgctgcggca aatatctgaa acctgtcgca taaaaatgcc gtctgaacgt
      601 tcagacggca ttttcaacac gcaaaaaaac cattcatacc aagggtaagt atgaatggtc
      661 aatacattgc gggaaaacgt cttacttgct gcactgccga aaagggagaa acggcagcgg
      721 taatcagcgg aaaggattgt acccgaatta atgttaagaa acgttaatcg tgaaaatata
      781 ttaacaaacc tgttgaacct attggttttc ccgtatccac tcgacccagc gttcaaacag
      841 cttcggttcg agcgcggcga cgaccgagcg tttgaacacg tgttcaccac tccaaaaccc
      901 gtcgccttcc aaagtcgtca gcctgccgcc cgcctcctcg aaaatcaacg cgccggcggc
      961 ataatcccac agcttctgcc cgccgtggac ataaacatca taacgcccgc acgccagata
     1021 acaccagtcc aacgtactgc tgcccatact ccgtatcgtt ccaaaaggcg cgagcgtact
     1081 catgcggctg gacagtttgc ccgaacgcag atatttgatt tccacacccg cgattgcctc
     1141 attcaacttc ttatccacga ggcgcaaagg caggcgtgtc ccattaagaa acgcaccctg
     1201 cccccgttcg gcataaaaac attcgccgct gaccggattg tagattgcgc ccaattcggc
     1261 gcacccgttg cggacaaacg ccaccgacac ggcaaaatgc ggcagcccgt tgacaaaatt
     1321 gttcgtcccg tctatcggat cgacaatcca cagccccttt tcccccgaat attgttccca
     1381 cagagccgac tgttcctgtc gcgacatttc ctcgcccaac atcgggcaat cgatcagaag
     1441 cggcaacgcg gcggcaaaag ccgtctgcgc ggcaatgtcc gcctcgctca acatcgaacc
     1501 atcttccttg cggtgggacg gcgtattcaa aaaacgcggc ataatttcgg tttgcgcgat
     1561 atggcgcacg actttctgca aacggtgtaa cacttcctac tgtcctcata ttttgaactt
     1621 gcggcacgcg aacgtatata gtggattaaa tttaaatcag gacaaggcga cgaagccgca
     1681 gacagtacaa atagtacggc aaggcgaggc aacgctgtac tggtttaaat ttaatccact
     1741 ataatgtccg tttccatcac gccgctgcga cagattataa ccgtccgaac cgccaaaaac
     1801 tatgccccga ttccacctgc ccgaaaacct ttccgtcgga caaaccgtca acctgcccga
     1861 caacatcgtc cgccacctca acgtcctgcg cgtccgcccc aacgaaaaca tcaccctctt
     1921 caacggcaaa ggcaaggcac acgccgcaca gttgaccgtt ttggaaaaac gccgcgccga
     1981 ggccgagatt ctacgcgaag aaacggccga tacagagtcc ccactcaaca tcacactgat
     2041 acaatccatc tcctccggcg atcgcatgga tttcaccctg caaaaaagcg tcgaactcgg
     2101 cgtaaccgcc atacagcccg tcatcagcga acgctgcatc gtccgcctcg acggagaacg
     2161 cgccgccaaa cgcctcgcac gctggcagga aatcgtcatc tccgcgtgcg aacaaagcgg
     2221 cagaaacacc gttccccccg tactgcccat catcggctac cgtgaagcac tcgacaaaat
     2281 gccgtctgaa agcaccaagc tgattatgag catcaaccgc gcccgcaaac tcggcgacat
     2341 ccgccacccg tccggcgcaa tcgtctttat ggtcgggccc gaaggcggct ggacagaaca
     2401 ggaagaacaa caggcatttg aagccggctt tcaggcggtt acactcggca aacggatttt
     2461 acgcacagaa accgccccac tcgccgccat cgccgcgatg cagacacttt ggggcgattt
     2521 cgcataaaca gaaatgccgt ctgaaacccg ttcagacggc attttgcagc cgattaagac
     2581 atagtaggtt caaataagat ttcccgtgtc gtcattcccg caacagcggg aatctagaaa
     2641 cgaaaaacta caaggactta tccgaaacaa caaaccctct ccgccgtcat tcccgcgca
//
LOCUS       DAWFIE010000131         2689 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWFIE010000131 DAWFIE010000000
VERSION     DAWFIE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.132"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            59..1432
                     /locus_tag="WGQ71_001991"
     CDS             59..1432
                     /locus_tag="WGQ71_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0707928.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNIHLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGNLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1737..>2689
                     /locus_tag="WGQ71_001992"
     CDS             1737..>2689
                     /locus_tag="WGQ71_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEZ0707929.1"
                     /translation="MLEKQHFPRFVIGESLLPHCMEMLEEAGFADAVRAEPGFQLKNG
                     AAFSWGSRYTEFDFTDKFSDGPGTTYQVRRAVFDKILIEEAAKQGVEVRFGHGVTAFD
                     NSGDFARLNIETDTGKRYELTAKFVLDASGYGRVLPRLLNLETPSHLPPRQAHFTHID
                     DNITNPKFDRNKILITTYPQHRDVWIWLIPFGDNRCSAGVVGTPDKLVGESETVLKKF
                     VYECPMLNEILDKAVWENDFPFRSIQGYSANVKSLHGRHFALLGNAAEFLDPVFSSGV
                     TIALHSAKLAADLLAKQLKGEAADWQTEFAEPLMIGVDAFRT"
BASE COUNT          671 a          842 c          674 g          502 t
ORIGIN      
        1 cccatcctat ctgtttcccg cttcagacgg cataaggtct gaaagaaaga ctacaattat
       61 gagtaatcca ttttcctctt taggtttggg tacggaactc gtttccgcac tgaccgcgca
      121 aggttacgaa aacccgacgc ccatccaagc cgccgccatt cccaaagcac tcgccggtca
      181 tgatttacta gccgccgcgc aaaccggcac gggcaaaacc gccgccttta tgctgccctc
      241 gctcgaacgc ctcaaacgtt acgccaccgc cagcacctcg cccgcgatgc accccgtgcg
      301 tatgctcgtg ttgaccccca cgcgcgaact cgccgaccaa atcgaccaaa acgtgcagtc
      361 ctacatcaaa aacctgccgc tgcgccacac cgtcttgttc ggcggtatga atatggacaa
      421 acagaccgcc gatttgcgtg ccggctgcga aatcgtcgtc gccacagtcg gacggctgct
      481 cgaccacgtg aaacagaaaa acatccattt gaacaaagtc gaaatcgtcg ttttggacga
      541 agccgaccgt atgctggata tgggttttat cgacgacatc cgcaaaatca tgcagatgct
      601 gccccgccaa cgccaaaccc tgctcttttc cgccaccttc tccgccccga tacgcaaact
      661 ggcgcaagac ttcatgaacg cgcccgaaac cgtcgaagtc gccgcgcaaa acaccaccaa
      721 cgccaacgtc gagcagcaca tcatcgccgt cgataccatt cagaagcgca acctgctcga
      781 acggctgatt gtcgatttgc atatgaacca ggtcatcgtg ttctgcaaaa ccaaacaaag
      841 cgtcgaccgc gtaacgcgcg aactggtgcg ccgcaacctg tccgcacagg cgatacacgg
      901 cgaccgttcc caacaaagcc ggctcgaaac actcaacgcc ttcaaagacg gcaacctgcg
      961 cgtcctcgtc gccaccgata tcgccgcgcg ggggctggat attgccgaac tgcccttcgt
     1021 catcaattac gaaatgcccg cccagcccga agactacgtc caccgcatcg ggcgcacggg
     1081 gcgcgcgggc gcggacggcg tggcgatttc cctgatggac gaatccgaac agaaaatgtt
     1141 tgaatccatt aaagagctga ccggcaacaa gctgctcatc gagcgcatcg agggcttcga
     1201 gccgcaatgg tgggaacagg gcggcgcaaa accggaaaaa cccgaaatgc gcgaaccgag
     1261 acaacgcaac cgctacgaat ccgccaaagc gcaacgcgaa aaaaacaccc ggccggaaaa
     1321 tgcggcaaac gatgcgggcg cggcttgcgg aaaaattgcc ggacgcagcc gccgaagccg
     1381 ccgggaacac cggacgtgcg ccctgctcca accgcgttac ggcgtaaaat agccctgaaa
     1441 atcaaatgcc gtctgaacat ttcccgtttc agacggcatt tttcaaaccg gactgacgca
     1501 tcgggagcaa ccgcccgcac cggataaatt tctgccgcaa acagtttcag acggcatttg
     1561 ccgcctgtac aatatagtgg attaacaaaa attaggacaa ggcggcgagc cgcagacagt
     1621 acaaatagta cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctttg
     1681 agctaaggcg aggcaacgcc gtactgattt ttgttaatcc actatatcaa gtctgcgtgt
     1741 tggaaaaaca gcactttccg cgcttcgtca tcggcgaaag cctgctgccg cactgtatgg
     1801 aaatgctgga agaagccgga tttgccgatg ccgtgcgcgc cgagcccggc tttcagttga
     1861 aaaacggcgc ggcgttttcg tggggcagcc gttataccga attcgatttc accgataaat
     1921 tttcagacgg ccccggcacg acttatcaag tgcgccgcgc cgtgttcgat aaaatcctga
     1981 ttgaagaagc cgccaaacaa ggcgttgaag tacgtttcgg gcacggcgta accgcgttcg
     2041 acaacagcgg cgattttgcc cgcctgaaca tcgaaaccga caccggcaag cgctatgaac
     2101 tgaccgcgaa attcgtcttg gacgcaagcg gttacggacg cgtgctgccg cgcctgctaa
     2161 acttggaaac gccctcgcac ctgccgccgc gccaagcgca tttcacgcac atcgacgaca
     2221 acatcaccaa cccgaaattc gaccgcaaca aaatcctgat taccacctat ccgcaacacc
     2281 gcgacgtgtg gatttggctg attcccttcg gcgacaaccg ctgttccgcc ggcgtggtcg
     2341 gcacgcccga caaacttgtc ggcgaatcgg aaacggtgtt gaaaaaattt gtttacgaat
     2401 gcccgatgct gaacgaaatt ttggacaaag ccgtttggga aaacgatttt ccgttccgct
     2461 ccatccaagg ctattccgcc aatgtgaaat cgctgcacgg caggcatttc gcgctgttgg
     2521 gcaatgccgc cgagttcctc gatcccgtgt tctcgtcggg cgtaaccatc gcgctgcact
     2581 ccgccaaact tgccgccgat ctgttggcaa aacaactcaa aggcgaagcc gccgattggc
     2641 aaaccgaatt tgccgaaccc ttgatgatcg gcgtggacgc gttccgcac
//
LOCUS       DAWFIE010000132         2660 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWFIE010000132 DAWFIE010000000
VERSION     DAWFIE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.133"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..194)
                     /locus_tag="WGQ71_001993"
     CDS             complement(<1..194)
                     /locus_tag="WGQ71_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0707930.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEG"
     gene            801..1610
                     /locus_tag="WGQ71_001994"
     CDS             801..1610
                     /locus_tag="WGQ71_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0707931.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDMPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEYEAYEDKRHIPENPYFHKLYYIKKGENPAIITHQNYHRYGENDY
                     STSVGSCINGFTVRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
     gene            1627..>2660
                     /locus_tag="WGQ71_001995"
     CDS             1627..>2660
                     /locus_tag="WGQ71_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707932.1"
                     /translation="MNEGEVVLTPEQIQTLYGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDTNLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGR
                     LPNTSEIERSYYKAVTENGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     VNNPNGSEWDNRKQLISALDKGFDGSFKEKPFTFLQSVIMDVTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVAKREWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKET
                     KKRNGKYHVYDPLALDLDGDGIETVATKGFAGALFDHRNQGIRTATGWVSADDGLLVR
                     DLNGNGIIDNG"
BASE COUNT          864 a          459 c          572 g          765 t
ORIGIN      
        1 ccctcaaaaa cctcatcggc agccaaggcc aaatgatggt tgataaccgt gcggatttta
       61 cggtagaaca gtgctgccga attgggatgg atacccaaaa tatcggcggc agaacgggcg
      121 gtaacttcga gtacaaaaaa acggagcagc tctttctgta cttttttctt taatttgcag
      181 tgcgttatct tcatatttcg agggtaacat atctgctaat ctagtacagc cccaaaaata
      241 taccaaaaac agcaaaacaa attgtaagga tacgtatagg ctttgtaaag gtaaattgtg
      301 aaaaaagcag ttttttaaac gaatgaaacg gcttcgggct gaaatatatg ctgatgccct
      361 gtccttcccg tatatcttgt gtgttgtcaa agtgcaggct gctttgaaat tggcattaca
      421 gtcgatgaac caccattttg ctttatttca gcgggcttga gatgtgtata agaatattgt
      481 tttgaataaa tttaaagaaa atgataatcg ttattgacga tttttaaagg aaagcgtaga
      541 gtgccaattc tatgaagcaa tacggtaagt aacaatgaaa atatctactg cttgggtata
      601 gagcatattt cacaacccgt aactattctt gcggaaacag agaaaaaagt ttctcttcta
      661 tcttggataa atatatttac cctcagttta gttaagtatt ggaatttata cctaagtagt
      721 aaaagttagt aaattatttt taactaaaga gttagtatct accataatat attctttaac
      781 taatttctag gcttgaaatt atgagaccat atgctactac catttatcaa ctttttattt
      841 tgtttattgg gagtgttttt actatgacct catgtgaacc tgtgaatgaa aagacagatc
      901 aaaaagcagt aagtgcgcaa caggctaaag aacaaaccag tttcaacaat cccgagccaa
      961 tgacaggatt tgaacatacg gttacatttg attttcaggg caccaaaatg gttatccctt
     1021 atggctatct tgcacggtat acgcaagaca atgccacaaa atggctttcc gacatgcccg
     1081 ggcaggatgc ttactccatt aatttgatag agattagcgt ctattacaaa aaaaccgacc
     1141 aaggctgggt tcttgagcca tacaaccagc aaaacaaagc acactttatc caatttctac
     1201 gcgacggttt ggatagcgtg gacgatattg ttatccgaaa agatgcgtgt agtttaagca
     1261 cgactatggg agaaagattg cttacttacg gggttaaaaa aatgccatct gcctatcctg
     1321 aatatgaagc ttatgaagat aaaagacata ttcctgaaaa tccatatttt cataaacttt
     1381 actatattaa aaaaggagaa aatccggcga ttattactca tcagaactat cataggtatg
     1441 gagagaacga ttacagcact agcgtaggtt cctgtattaa cggtttcacg gtacggtatt
     1501 acccgtttat tcgggaaaag cagcagctca cacagcagga gttggtaggt tatcaccaac
     1561 aagtagagca attggtacag agttttgtaa acaatccaag taaaaaataa tttaaaggat
     1621 cttattatga atgagggtga agttgtttta acaccagaac aaatccaaac cttgtatggt
     1681 tatgctttcc gtggcgatac ctatggcggt tggcgttatt tggctaattt gggtgaccgt
     1741 tatgcggatg atgctgccgc aattgtcggt aaggatacaa acttaaatgg tttgaattta
     1801 tggatgaaaa aaggtgtgga aaacctatgg gatgatacgg tcggtaaaaa gacccgttta
     1861 gagaaatttg atcgggttgc actgcaacat ttcagccaat atgtagatct aattaatgaa
     1921 aataatggta gattacctaa cactagtgaa attgagagaa gttactataa agccgttacc
     1981 gaaaatggtg tttcttctag tgcagctatt gatttagtta ttaatcgttc acttccggat
     2041 atggcggatg gttattgggc attaggtttg gggatagaag ccgaacgtat ccacaatgag
     2101 caagcagtaa ataatccgaa cggtagcgaa tgggataata gaaagcagtt aatatctgct
     2161 ttagataaag gatttgatgg atcttttaaa gagaagcctt ttactttttt acaatctgtg
     2221 ataatggatg taacaaagtt aggtgttgaa tatacaatag atggttggca aaaaattgga
     2281 ggttggggta atgggataat caatgattta tataaaagtg ttgcaaaaag agagtggact
     2341 ggaatatttg agatcgttaa taataacatc aagcaattta gagatctgtt cccaaatccg
     2401 gaaggctgga tcgatgatgg tcaccaatgt ttcgctcctt gggttaaaga aactaaaaaa
     2461 cgcaatggca aatatcatgt ctacgacccc ctcgccctag atctagacgg cgacggtata
     2521 gaaaccgttg ccaccaaagg ctttgcaggt gcattgttcg accaccgcaa tcaaggcatc
     2581 cgcaccgcca ccggttgggt ttctgccgat gacggtttac tcgtccgcga tttgaacggc
     2641 aacggcatca tcgacaacgg
//
LOCUS       DAWFIE010000133         2657 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWFIE010000133 DAWFIE010000000
VERSION     DAWFIE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.134"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(417..2051)
                     /gene="groL"
                     /locus_tag="WGQ71_001996"
     CDS             complement(417..2051)
                     /gene="groL"
                     /locus_tag="WGQ71_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ0707933.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGTVIAEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGMGGMM"
     gene            complement(2144..2434)
                     /locus_tag="WGQ71_001997"
     CDS             complement(2144..2434)
                     /locus_tag="WGQ71_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ0707934.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT          560 a          710 c          681 g          706 t
ORIGIN      
        1 tccatccgca cagaaacctg caccacgtca ttcctacgaa cctacatccc gtcattccca
       61 caaggacaga aaaccaaaat cagaaaccta aaatcccgtc attcccacga aagtgggaat
      121 ccagaacgta aaatctgaag aaaccgtttt atccgatacg tttccgcacc gacagaccta
      181 gattcccgct ttcgcgggaa tgacggcgga aaggttgctg tttttccgat aaattcctgc
      241 cgctcttcgt ttttgggatg gcgggaaata aaacaaaagc gcgcgtatca aaaaataaca
      301 atgcaaagaa cggtgttggc aattttaagg tttgcgcctt agccgcactt attcgcaaaa
      361 aaaccgcacg gcgttgaccg tgcggcttgt tgtctgaagg cttcagacgg cattgcttac
      421 atcatgccgc ccataccacc catgccgccc atatcaggca cagccggttt gtcttcgggg
      481 atttcagcga tcatgcaatc agtggtcagc atcaagccgg cgatggaagc ggcgtgttgc
      541 agcgcggaac gggttacttt ggcggggtcg agtacgccca tttcgatcat gtcgccgtat
      601 tcgccggagc ctgcgttgta accataattg cctttgcctt ccaacacttt gttcaccacc
      661 acgctgggtt cgccgcctgc gttggcaacg atttggcgca gcggagactc aacggcgcgc
      721 aagacgattt gtacgcctgc gtcttggtcg gcattgccgg tgtgcaggtt ttccaaagca
      781 gcacgggcgc gcaacagggc tacgccgccg cctgcaacca cgccttcttc aacggctgcg
      841 cgggtagcgt gcagcgcgtc ttccacgcgg tcttttttct ctttcatttc aacttcggtc
      901 gccgcgccga ctttgatcac tgccacgccg cctgccagtt tggcaacgcg ctcttgcagt
      961 ttttctttgt cgtaatcgct ggttgcggtt tcgatttgtt ggcggatttc ggcaacacgc
     1021 gcttcgattt gggctgcgtc gccgaagccg tcgatgatgg tggtgttttc tttaccgatt
     1081 tcgatgcgtt tggcttgacc caaatcgtcc aaagtcgctt tttccagaga caggccgact
     1141 tcttcggcaa ttacggtgcc gccggtcagg atggcgatgt cttgcaacat ggctttacga
     1201 cggtcgccga agccaggggc tttaacggca acggttttca ggatgcctcg gatgttgttc
     1261 accaccaaag tcgccaaggc ttcgccttcc acgtcttcag cgataatcaa gagcgggcgg
     1321 ctggctttgg ccacttgttc caaaacaggc aacaaatcgc ggatattgct gatttttttg
     1381 tcgaacaaca atacaaacgg attgtccaaa gcagcgattt gtttttcagc gtcgttgatg
     1441 aagtaaggag acaggtagcc gcggtcgaac tgcatacctt caaccacatc cagctcgttt
     1501 tccaaagatt tgccgtcttc aacggtaatc acgccttctt tgccgacttt ttccatcgct
     1561 tcggcgataa tcgcgccgac ttgttcgtca gagttggcgg aaatcgagcc gacttgggcg
     1621 atttctttag aagtgtcgca aggtttggcg atgtttttca gctcgtcaac caaagcggca
     1681 acggctttgt cgataccgcg tttcaggtcg gtcgggttca taccggcggt aacgtatttc
     1741 ataccttcgg caacgatgga ttgcgccaat acggtggcgg tagtcgtacc gtcgcccgcc
     1801 acgtcgttgg ttttggacgc gacttctttc accatttgcg cgcccatatt ttcaaacttg
     1861 tctttcagtt cgatttcttt ggcgacggtt acgccgtctt tggtgatgtg cgggccgccg
     1921 aatgcgcggt caaccactac gttgcggcct ttgggaccca aggttacgcg gacggcgttt
     1981 gccagaatgt tcacgccgtt taccattttt tgacggactt cattgccgaa ttgtacgtct
     2041 tttgctgcca tttcaattct ccaaaaatca ttaaaactgt ctgataaaac cgtttatgcc
     2101 gtctgaaggc ggtttgccgt ttcagacggc atcgtgtccg tatttatttt tcaacgatgc
     2161 cgaagatgtc ttcttcgcgc attaccaaca gctcttcgcc gtcggctttg acggtttgac
     2221 cgctgtattt gccgaagatg attttgtcgc cgactttgac atccagcgga cggcgcgcgc
     2281 cgtctttacc gattttgccc gcgcccacgg cgatgacttc gcccatgtcg ggtttttcgg
     2341 cggccgcacc cggcaaaacg atgcccgatg cggttttctc ttcagcttcc aagcgtttga
     2401 cgacaacgcg gtcgtgtaaa ggacggatgg tcatatttat gctccgataa aatgatttga
     2461 aaacaatcat ctgcccgaac ggttcggaca gattgaagtg gaaaccggac agccgtcagg
     2521 cggctgcccg tataagtcgg caaaattagg gtgtgtgcgg gcaaattcaa gtgaggcgga
     2581 aaaaatttat ttccgccgtt ttttatagtg gactaaattt aaggggctgt actagattag
     2641 cagatatgtt accctcg
//
LOCUS       DAWFIE010000134         2598 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWFIE010000134 DAWFIE010000000
VERSION     DAWFIE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.135"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            99..656
                     /locus_tag="WGQ71_001998"
     CDS             99..656
                     /locus_tag="WGQ71_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ0707935.1"
                     /translation="MGFGCREGKEFCKGLSLPEIIRDLLEMPSETAVADIARTYSAHY
                     LNPNNRNMTLFPDALPCLDKLKAQGYWLAVATGKGRAGLDGAISQTATGGYWLATACA
                     GEYPSKPSPEMVFGICRELGLDPKEALVVGDTAHDLHMAANAGAAAVGVTTGAHSREQ
                     LLSAPHLAVLDGLSELPGFLARHYA"
     gene            complement(743..1711)
                     /gene="ispH"
                     /locus_tag="WGQ71_001999"
                     /gene_synonym="lytB"
     CDS             complement(743..1711)
                     /gene="ispH"
                     /locus_tag="WGQ71_001999"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ0707936.1"
                     /translation="MNGKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLIYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAAGIGTDAYMVDNASYLQRAWFEGKNKVGVTAGASAPEVLV
                     QEVLATIRRWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1741..2235)
                     /locus_tag="WGQ71_002000"
     CDS             complement(1741..2235)
                     /locus_tag="WGQ71_002000"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ0707937.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     GKKT"
BASE COUNT          579 a          788 c          665 g          566 t
ORIGIN      
        1 gcagcccgga tgcctgatta tcgggtatcc ggggattaag ggggtgtttg ggtagaatta
       61 ggaggtgtta tggggcgaaa acagccgaaa acctgtgttt gggtttcggc tgtcgggagg
      121 gaaaggaatt ttgcaaaggt ctcagcctgc ccgaaatcat ccgcgacctg ctcgaaatgc
      181 cgtctgaaac cgccgttgcc gacatcgcgc gcacttattc cgcgcattac ctcaatccca
      241 acaaccgcaa tatgacgctc tttcccgatg ccctgccctg tctggacaag ctcaaagcac
      301 aaggatactg gcttgccgtc gccacgggca aagggcgggc gggcttggac ggtgccatca
      361 gccaaaccgc caccggcggc tattggctcg ccaccgcctg cgcgggggaa tatccctcca
      421 aaccctcgcc cgaaatggtg ttcggaatct gccgcgaact gggactcgac ccgaaagagg
      481 cattggtcgt cggcgatacg gcgcacgacc tgcatatggc ggcaaacgcg ggtgcggcgg
      541 cagtcggcgt taccaccggc gcacattcgc gcgaacagct ccttagcgca ccgcatctcg
      601 ccgtattgga cggtttgtcc gaactgcccg gttttcttgc acgacattac gcctgatcgg
      661 tttccgcatc cggcacgcgg caaaaatgcc gtctgaagcc tgttcagacg gcatttgtgt
      721 tgcccaaaca tccgacgctt gctcaacgtt tgcacaaatc gggtttggtt tcgccctcgc
      781 ggcgcagctc tttgggcagg acgaacacaa tgctttcttc cgcgccctcc ccttcgcgta
      841 cggtttcgtg cccccatctg cgtatggttg ccagtacttc ctgcaccaac acttcgggcg
      901 cggacgcgcc tgccgttacg ccgactttgt ttttgccctc aaaccacgcg cgttgcaggt
      961 aggacgcgtt gtccaccata tacgcatcgg tcccgatgcc tgccgccact tcgcgcaggc
     1021 gattgctgtt ggacgaattg ggcgaaccga ccacaatcac gatgtcgcac tgttccgcca
     1081 gctctttgac ggcggtttgc cggttggtcg tcgcatagca gatgtcttcc ttgtgcggat
     1141 tgcggatatt ggggaaacgc gcgttcagcg cggcaatgat gtctttggtt tcatcgaccg
     1201 agagcgtggt ttggctgaca taggcgagtt tgtcggggtt tctgacttcg agttttgcca
     1261 catctccgac cgtttcgacc aaaagcattt tgcccggcgc aagctgcccc atcgttcctt
     1321 cgacctcaac gtgcccctta tgcccgatca tgatgatttc acagtcttgg gcatccagtc
     1381 gggcgacttc cttatgcact ttcgtaacca gcgggcaagt cgcatcaaac acgcggaaac
     1441 cgcgctccgc cgcttcctgc tgcaccgcct tcgatacacc gtgtgccgaa taaatcagcg
     1501 tcgcgcccgg cggcacttcc gccaagtctt cgataaacac cgcgcctttt tcacgcaggt
     1561 tatcgacgac gaatttgttg tggacgactt cgtggcgcac ataaatcggc gcgccgaatt
     1621 cttccaaagc acgctcgaca atactgattg cccgatccac gccggcgcag aagccgcgcg
     1681 gattggcaag gatgatggtt tttccgttca taagttttac attccgtgtt cagacggcat
     1741 tcacgttttt ttgccatctt tgcgatggac gatattgtcc aacaccgcca acaccgcacc
     1801 gacgcagata aagctgtcgg caatattaaa ggcgggataa aaccaatttt gccaataaaa
     1861 caataagaaa tcgacgacat gaccgtgtat caggcgatcg atgacattgc ctaacgcacc
     1921 gccgataatc atcgccgcac ccgttttgcc gagggttgca aactcatcgc gcaagatggc
     1981 gcgtaccaaa tacgcgctca ccgccaccgc cagcaccaaa aaaaagtatt tttgccagcc
     2041 gccctgatcg gcaaggaagc tgaacgccgc gcccgggttg tacaccaagg tcagatcgaa
     2101 aaacgaagga atgacattga cgcgttcccg atactgaaac gacgacagca ccgcccactt
     2161 cgaccactgg tccagcacga tggcggcaag tgccaatacc caatagcgcg ttttacttga
     2221 aacagatgaa gacatatttt tcaacagccg gataaaagag tgccatttta cccgaaaacc
     2281 ccctgcctgt acccgaaacg gcaaatgccg gaatcttgaa acccgtcatt cccgacaaca
     2341 ccgtaatctc gaaacccgtc attcccgcgc aggcgggaat ccagacctgt ccgcacagaa
     2401 acttatcgga taaaaacagt tgctcaaacc cctcgttcta gattcccact ttcgtgggaa
     2461 tgacggttca gttgcgttcc aacaacaccg caatctcgaa atccgtcatt cccgcgcagg
     2521 cgggaatcca gacctccgac gcggcgggaa tctatcggaa atgactgaaa ccccgagatt
     2581 ctagattccc actttcgt
//
LOCUS       DAWFIE010000135         2446 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWFIE010000135 DAWFIE010000000
VERSION     DAWFIE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.136"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(425..2305)
                     /locus_tag="WGQ71_002001"
     CDS             complement(425..2305)
                     /locus_tag="WGQ71_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEZ0707938.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAYRPSISIYFDVDSE
                     FNVGAPTCKIEAVNIAENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGNVSVVRRERGSPIDTLVSEMMILANSTWAQMLD
                     ENELPGLFRVQPAGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     SAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          490 a          599 c          730 g          627 t
ORIGIN      
        1 atttccacct gcgcgggaat gacgattcag aagttgcccg aaacctaaaa aactgaaacc
       61 gaacgagccg gatttccgct ttcgcgggaa tgacggcgta taagttcccg tgcggacaga
      121 cctatattcc cacctgcgcg ggaatgacga ttcagaagtt acacgaaact caaaaaaaac
      181 gaaaccgaac gaaccggatt cccgctttta cgggaatgac ggcgcataag ttcccgtgcg
      241 gacagaccta gattcccacc tgcgtgggaa tgacgggatt ttagattgcg ggtatttatc
      301 gggaacggcg gcttggaagt tcattgaaac ggaaaaacaa cggaaaccca aaaaaccgga
      361 ttcccgactg tgggaatgat gagattcagg tttctgtttt tgccggagtt tgccgtatcg
      421 ggcttcagac ggcattgcct gccgttgtac ccgcgggtgc gactgccttg atgtagttga
      481 gcgagacaaa ctgcttctcg gcatccaatt cggtgatttt gaacaatgcc tgtgatttgg
      541 gcagcgcgtc aaacggaata ccggtcgcgc gcgttaccag cggcaggcct tcaatgcgga
      601 cgaggtcttc tttgaggatg gtggccgtca gctcgctcgt gccttgctgt tgcagataga
      661 cgaggctcca gtaggcttcc atttggcgtt ggaagtcggc gtaggcggta taggcggcat
      721 caaagtcgcg cagcgcggcg aaaagctcgg catcgctgtt ttgatacagc ggctcggcag
      781 agtcgtctat caggctgatc agctgctttt ggttgatgta gtcggcggcg cggcgcagcg
      841 gcgaagtgaa ccagccgtaa tgctgcacgc ccatgccgat gtgcggctcg gatttggtgc
      901 tcatgcgcac tttgcctgcc ggttggacgc ggaaaaggcc gggcagctcg ttttcgtcca
      961 gcatttgcgc ccaagtgctg ttggcaagaa tcatcatctc gctgaccagc gtatcgatgg
     1021 gcgaaccgcg ttcgcggcgg acgacggata cgttgccttc ctcatccaat tcgatgctgt
     1081 aatcgtattg cggcgcacgg tcgggctcgt atttgccgcg cgctttttgc agggcggtgg
     1141 cgaattgata gaaccaaatc aggtcttgat ggtgggcgaa catcatttcg cccgcttcgt
     1201 ccaagcccgc ttcggcgttg aaatgcggtt cgatggcttg gatacgcagg ttttcggcga
     1261 tgttgaccgc ttcgattttg caggtcggcg cgccgacgtt gaactcgctg tccacatcga
     1321 aataaatgct gatggaaggg cggtatgcac ctgcatcaag gctgaacgcg gcaatccagt
     1381 tttcgggcag catcgtgatt ttgccgccgg ggaaataaac cgtgctcaag cgttccatga
     1441 tgtttttttc cattttgtcg ccttgtttaa tggcaagcga cggcgcggcg atgtggatgc
     1501 cgacgcgctt cgtgccgttg tccaagtcgg tcaggcttaa agcgtcgtcc acttcggtgg
     1561 tcgattcgtc gtcaatggaa aaggcggtaa cgtcagcctt gggcaggtcg ggcatttcgg
     1621 gaagcggaag gtcggggaag cctgttcctt tggggaagta tttgatttca aacccgtctt
     1681 gcaggtattg gggaatggac gtaatgccgc ccgttttttt cgccaattcg taggcagagg
     1741 ttttcagcgc gtcggcggct ttggtaaagg ctttgtaggt cagcgactgc ttgtcgggcg
     1801 cgtgcaggat ggttttcaga tccgccgcga tttcagacgg catctcgccg cgtttcaagg
     1861 cttccgccca agcgtcgatt tgcgcgtctt gctgtttttt gcgttcgatg gcggcaagtg
     1921 cctgtttcaa agtttcttcg ggcgcggctt tgaacacgcc tttggctttt ttgtagaaat
     1981 acatcggcgc ggcgtacagc gcaatcaagg tcgccgccag ctcggttttg gtcggcgcat
     2041 ggccgtaata ttcttcggct atggcttcag cggtaaattc ctcttcgccg catacttccc
     2101 acaataaatc ggtgtcgatg tccgccgcct gtgcctgcgc gttttccaaa aacgccgcca
     2161 tatcgccgtc aaactcggca aagacgttgt tcgccttcac tttggtgcgt ttgccgtgtg
     2221 gggtatcgac ttggtaggtg gcatcgtttt tttggatgat ggcggcgatt ttgaattggc
     2281 cggactcttc gtaaaaaata ttcatttttc ggatttttct gtggaaactc aagcgggcga
     2341 ttttagcaga ttaccgaaaa tgccgtctga aaaaaggttg ggagagggtt ggcgcgtctt
     2401 tgcggtgctt gcgttatagt ggattaacaa aaaccagtac agcgtt
//
LOCUS       DAWFIE010000136         2434 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWFIE010000136 DAWFIE010000000
VERSION     DAWFIE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.137"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            35..1390
                     /locus_tag="WGQ71_002002"
     CDS             35..1390
                     /locus_tag="WGQ71_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ0707939.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLFYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKSY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLIITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMMGGLQANMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="WGQ71_002003"
     CDS             1394..2404
                     /locus_tag="WGQ71_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ0707940.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     TYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDGLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRCRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLHWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          455 a          657 c          726 g          596 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt taggcggccg
      301 ccgtctgccg tatctgtttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcgaa tatggcgatg cagccgttta agatgatggt
      481 cggcgatatg gtcaacgagg agcagaaaag ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg ttcgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccaca aaccgtggtc gtagcattct atgtgggcgc
      661 ggcattactg attattacca gtgcgttcac aatcttcaaa gtcaaagaat acgacccgga
      721 aacctatgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggtt
      781 cgaactctta aaaacggcac ctaaagcgtt ttggacggtt actctggtac agtttttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccact gatgcgtctt ccgtaggtta tcaggaggcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtaacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgccgcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tcccgatgat gggcggtttg caggccaata tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttccgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat ttcggtctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgat gatgcggctt cctatgcgga caatccgttt cagattaaca aacagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttacctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag acggcctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgctgtcg tatcagcata tacagcgacc gcaacggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgcat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgcagacg gcct
//
LOCUS       DAWFIE010000137         2308 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWFIE010000137 DAWFIE010000000
VERSION     DAWFIE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.138"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WGQ71_002004"
     CDS             complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WGQ71_002004"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ0707941.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGNGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1627..2193
                     /locus_tag="WGQ71_002005"
     CDS             1627..2193
                     /locus_tag="WGQ71_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0707942.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          528 a          564 c          663 g          553 t
ORIGIN      
        1 tcctagttat ctaggacagc cccttaaatt taatccacta tattttcctg tttcaggctc
       61 ggcgttcttt gcacatcagt ttcgcatcca gccagccatc gccgccggag atgatgttgt
      121 tggcaacgaa gttgtccaag tcgggcagca gggaaacggc gcggccgctg ttgatgatga
      181 cgaccaccgc catcggtttg tcgcccagcc aataacctgc aagggcgcgg acattgttga
      241 gcgtgccggt ttttaagcgc aacagcccgc cgctttgttt gaagcggttg cgtaaagtcc
      301 cgtctgtgcc ggcgatgggc agcgtatcga tgaaatcttg tgcaaacggg ctgaaataag
      361 ccgtttccaa catttgcgcc atcattctcg ccgttaccct ttcttttcta gacagacctg
      421 aaccgttttc caaaaccaaa tccgcaacat cgatgcccga cacggcaagt tcacgccgga
      481 cggcagacgc tgcctgttcg gaaacggcgg gcagtttgcc gttgccgccg agtttgagga
      541 agacggaacg cgcaattaga ttgtccgaac gcttgttcat gtccgtcaaa atttccttca
      601 tcggctttga gtgtgcaacg gcaagcgtct gcgcgccttc cggcgtgtcg gatatgccga
      661 tgccgtctga aatccgtccg ccgccgagca gccagtggtt ggtaaaactt tgccggatca
      721 gttcgtcaag cgcgaacatc cggacaccga caggcttgcc caaacagctc tcgggaatat
      781 tgccgcgcaa tttcagcgta ttgtccgaaa aagatgcacg catcagtttt ttgatcgaag
      841 ggcaggcagc ttgggaggcg gtaattttca agttgttttg ggcgaaaata tgcggcaaag
      901 gcggatcggt gaggatgtcg gtactgtcgg cggcattgcg ttcggcgcgc accataacca
      961 taccggcaga cagcatagtt ggattggggg gcgtcataaa cggcgaaccg ctgtcggctt
     1021 cgaaatcgtc ggggctgccg acttcgcccc acaggctgtg gtcgagcatc aggtgtcccg
     1081 tgatattgcg tatgccttgt tcgcgcaact ggcgttggac ggcaagcagg ttttcctgat
     1141 tgaaaacggg gtcgccgctg cccgcccaat acaggtttcc gtcaagcgtg ccgtcgttta
     1201 ccgtaccgtt gcttttaaac tcggtcgccc agcggtaatt gctgccgaag gttttgaagg
     1261 cggcaaacgc ggtaacgagt ttcattgtgg aggcggggtt gacggggaca tccgagcggt
     1321 ggtcaatgat gacttttccg ctgtcaagct cttggacata tacggcgatt tcgttttgcg
     1381 gaatgcgacc cgtatcgagc gcgtgtgcgg cgagggaggc gagaagcagc agcagggagg
     1441 ccgctgtttt ggggaaattc ataggtaaat ctgttacata aaaaaagcaa ttataaggca
     1501 aagccggata agtgggaacg ctgtggcggc gggccggctt gtttgcaggg gaaatcacat
     1561 atataataat cgctatcatt atgaaatgat tgagacgcac aaacttaata ttcaggagga
     1621 atgattatgg ctaagaaaat cagtattttg gtgggcagcc tgcgccgtgc ttcttttgcg
     1681 cgcaaagtgg cattgaatgc ggcggagatg ttccccgaag gctggcaggc ggaaatcgtc
     1741 gaaatcggac atctgccgct ttacaatttc gattatgacg accctgcggt ggaagatgtg
     1801 ccgctgcccg aaagctacac ggttttccgc gaaacgatta aggcttcgga cggcattttg
     1861 ttcgttacgt ccgaaaataa ccgcaccatt cccgcctgtt tgaaaaatgc ggtggacatc
     1921 ggctcgaaac cgaatgccga cgtggcgtgg aaaaacaaac cggccggcat catcagccat
     1981 tccgtcggca agatgggcgg ttacagttcg caaaaaaacc tgcgccttgc gctgtcttat
     2041 ttcgatatgc ccgtaaccgg acagccggaa gtgtttttgg gcaattcgcc gacgctgttt
     2101 gatgaaaacg gcaaattgat cgactcggca agggattttg ttcagtcata tattaatcag
     2161 tttgtcggtt tgattgaaag aaatgccaaa taaacaaaca agtcgaaagg ccgcaaaacc
     2221 ggttcgggtt ttgcggcttt tttatatggg ctgggcagcg tcaaggtttg ccgtttgccg
     2281 gataatgcgc ggcaaatgac cggcgtgt
//
LOCUS       DAWFIE010000138         2292 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWFIE010000138 DAWFIE010000000
VERSION     DAWFIE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.139"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            145..531
                     /locus_tag="WGQ71_002006"
     CDS             145..531
                     /locus_tag="WGQ71_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ0707943.1"
                     /translation="MKGLDKYILFKNCETLGELFRIYQDYLSLNIDDPNLKIKFFDLL
                     EELLSDGVIELCDYRENPPKILTGSPKTQVDELRKIWPDMEEMLLYFPDNPWHYVEWF
                     WWGATCPIELTQLPKIEIYEEQMKQA"
     gene            559..2154
                     /locus_tag="WGQ71_002007"
                     /pseudo
     CDS             559..2154
                     /locus_tag="WGQ71_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
BASE COUNT          808 a          530 c          518 g          436 t
ORIGIN      
        1 accttggaaa ggaacgacca tcattttaag gaatttttca actagccgag aacaaactgg
       61 agcaaaatgg acgattgagt ttcaaaatca tccattagta ggtaggaaaa aaatggaatt
      121 aaaatttaga taaggaattg aataatgaaa ggtttagata agtatatcct atttaaaaat
      181 tgtgaaacat taggagaatt gtttcgaata tatcaagact atctatcatt aaatattgat
      241 gacccaaatt taaaaattaa attttttgac cttttggaag aacttttatc agatggtgtt
      301 attgaattat gcgattatcg tgaaaatcct ccaaaaatat taaccggttc gccaaaaaca
      361 caggtagatg aattaagaaa aatttggccg gatatggaag agatgttgct atattttcca
      421 gataaccctt ggcattatgt tgaatggttt tggtggggag caacttgccc tattgaactg
      481 acacaattgc ctaaaataga aatttacgaa gaacaaatga aacaagctta aagccagcgt
      541 aaccagcctg tatgacaagg tgcaggtgaa caatccaata tccacattac cggctcagat
      601 gttgccggca aagaaggaac cggcctgatt gcagataagc aaatccatct gcaatcagcc
      661 gagcaaagta ataccgaacg cagccaaaac aaatcggcag gctggaacgc aggtgctgcc
      721 gtatcattcg gacaaggagg ctggtcatta ggcgttaccg caggcggcaa tatcggcaaa
      781 ggccacggca atggcgacag catcacccac cgccatagcc atatcggcga caaaggcagc
      841 caaaccctta tccaaagtgg tggcgatact accatcaaag gcgcgcaagt acgcggcaaa
      901 ggcgtacaag tcaatgccaa aaacctaagt attcaaagcg tacaagatag cgaaacctat
      961 caaagcaaac aacaaaacgc cagtgcacaa gtgaccgtcg gttatggctt cagtgccagt
     1021 ggcgattaca gccaaagcaa aatccgagcc aatcatgctt cggtaaccga gcaaagcggt
     1081 atttatgccg gagaagacgg ctatcaaatc aaggtcggaa accatacaga cctcaaaggc
     1141 ggcatcatca ccagtaccca aagcgcagaa gacaagggta aaaaccgatt cagcacaggc
     1201 acactcgccg gcagtgatat tcaaaattac agccaatacg aaggaaaaag ttttggattg
     1261 ggcgcaagtg cgtccataag cggcaaaaca ctgggacagg gcgcacaaaa taagcctcaa
     1321 aacaaacacc tgacaagcgt ggccgataaa aacggcgcaa gttcatcagt aggctacggc
     1381 agcgacagcg acagcgacag ccaaagcagc atcacaaaaa gcggcatcaa tacccaaaac
     1441 attcaaatca ccgacgaagc cgcacaaatc cggctgacag gaaaaacagc ggcacaaacc
     1501 aaagccgata ttgatacaaa cgtaaccaca gacaccgccg aacgacattc gggcagcctg
     1561 aaaaacgtat ttgacaaaga tagagtgcaa agtgaactgg atttacaaag aaccgtcagc
     1621 caagatttta gtaaaaatgt tcaacaagcc aataccgaga ttaaccaaca tttagacaaa
     1681 ctcaaagcag acaaagaagc agccgaaaca gcagcagccg aggcattagc caatggcgat
     1741 atggaaactg ccaaacacaa agcccatgaa gctcaagatg cggcagcaaa agcagataat
     1801 tggcaacaag gcaaagtcat tctcaacatg ttagcctcag gtttagctgc gccgacccaa
     1861 agcggagcgg gcatcgctgc ggctaccgca tcgccagccg tatcgtatgc gattggacag
     1921 cactttaaag atttagccgg tcaaaacgcg aatggcaaac taaccgccag tcaagaaacc
     1981 gctcacgttc ttgcccacgc ggtattagga gcagcggttg cagcagcagg aggcaacaat
     2041 gccccggcgg gagcattgag tgcgggcggg tcggaagcgg ctgcgcctta catcagcaaa
     2101 tggttatatg gcaaagaaaa aggaagtgac ttaacggcgg aagagaaaga gactgagacc
     2161 tttgcaaaat tccccaaaat cccctaaatt cccaccaaga catttaggag atttctcatg
     2221 agcaccttct tccagcaaac cgcccaagcc atgattgcca aacacatcga ccgtttcccg
     2281 ctattgaagt tg
//
LOCUS       DAWFIE010000139         2199 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWFIE010000139 DAWFIE010000000
VERSION     DAWFIE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.140"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            33..518
                     /locus_tag="WGQ71_002008"
     CDS             33..518
                     /locus_tag="WGQ71_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707944.1"
                     /translation="MFVNEKYPYAALFAGLVFLTLPFALSVHDALAFAFGRAGLLVSV
                     SDGGFGWRGGWDGTVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAAAVAWIFELLLGSEALGGLRGEAVSEYAFAVWLVAMLTLPKRLTRAPVQPVVFHRK
                     K"
     gene            614..1237
                     /gene="rsmG"
                     /locus_tag="WGQ71_002009"
     CDS             614..1237
                     /gene="rsmG"
                     /locus_tag="WGQ71_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ0707945.1"
                     /translation="MERKERLRAGIAAMGLDILETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVITSRAFAELADFVSWTGHLLKDG
                     GYWAAMKGVYPQGEIDRLPQDVCVEKVQRLDVPGLDAERHIAILRKR"
     gene            1336..2109
                     /locus_tag="WGQ71_002010"
     CDS             1336..2109
                     /locus_tag="WGQ71_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ0707946.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVVLGDADVKSATVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELMARVSGK"
BASE COUNT          441 a          501 c          748 g          509 t
ORIGIN      
        1 aattcggtct gcttcggaag agggggacgg cgatgtttgt gaacgagaaa tatccctatg
       61 cggctttgtt tgcggggcta gtgtttttga cgctgccgtt cgcactgtcg gtgcatgatg
      121 ccttggcatt tgcgttcgga cgggcggggc tgctggtgtc ggtgtcggac ggcggattcg
      181 gctggcgtgg cggttgggac ggcactgttt ggtttgtgtt cggtgtgttt gcgtttttga
      241 atgtggttgt atcggcgggt ctgacgaaac tggcgtacaa aaagatgatg cggcggcatt
      301 cccgttacgc gctgtttctg tcgggcgtgg cggcttgcgc ggcagcagcg gtggcttgga
      361 ttttcgagct gttgttgggc agtgaggctt tgggcgggct gcgcggggag gcggtgtcgg
      421 aatatgcgtt tgccgtgtgg ctggtggcga tgctgacgct gcccaaacgc ctgacgcgcg
      481 cgccggtgca gccggtggtg tttcacagga aaaaataggt tggaacggga atgccgtctg
      541 aaacgccgac acgcggtttc agacggcatg tttttccgtt aacattacgc ctgaatatgg
      601 ataggaagca gatatggaac gtaaagaacg cctgcgtgca ggcattgccg cgatggggct
      661 ggatattttg gaaacggcgc aggacaggct tttggcctat gtggatttgt tgaaaaagtg
      721 gaacaaaacc tacaatctga ccgcactgcg cgacgaggaa aaaatgattg tccatcatct
      781 tttggacagc ctgacgctgc tgccctatat cgaaggcgca cagacgatgc tggatgtcgg
      841 ttcgggcggc ggccagcccg gcattccggc ggcggtgtgc cgtccggatg tgcagataac
      901 ccttttggat gcgaatacga agaaaacggc ttttttacag caggcggtta tcgagttggg
      961 attggacaat gtgcgcgtgg tatccggacg cgtggaggcg gtttcggacg tgcgtgccga
     1021 cgtgattacc agccgtgcgt ttgcagaact ggcggatttt gtgtcgtgga cggggcatct
     1081 gttgaaagac ggcggctact gggcggcgat gaagggcgtg tatccgcagg gggaaatcga
     1141 ccgcctgccg caggatgtgt gcgtcgaaaa agtccaaagg cttgacgtgc cgggcttgga
     1201 tgcggaacgc catatcgcca tactccgcaa gcgttgagcg cgcttcagac ggcatgaata
     1261 ccttttttgt gcggataaag gtaaaattcc gcactgtttt tcttttttca acatcagacg
     1321 ggacacgggc gggacatgag tgcgaacatc cttgccatcg ccaatcagaa gggcggtgtg
     1381 ggcaaaacga cgacgacggt aaatttggcg gcttcgctgg catcgcgcgg caaacgcgtg
     1441 ctggtggtcg atttggatcc gcagggcaat gcgacaacgg gcagcggcat cgacaaggca
     1501 agtttgcagt ccggcgttta tcaggtcgta ctgggcgatg cggacgtgaa atcggcgacg
     1561 gtacgcagca aagagggcgg atacgctgtg ttgggcgcga accgcgcgct ggccggtgcg
     1621 gaaatcgagc tggtgcagga aatcgcccgg gaagtgcgtt tgaaaaacgc gctcaaggca
     1681 gtggcggaag attacgactt tatcctgatc gactgtccgc cttcgctgac gctgttgacg
     1741 cttaacggct tggtggcggc gggcggcgtg attgtgccga tgttgtgcga atattacgcg
     1801 ctggaaggga tttccgattt gattgcgacc gtgcgcaaaa tccgtcaggc ggtcaatccc
     1861 gatttggaca tcacgggcat cgtgcgcacg atgtacgaca gccgcagcag gctggttgcc
     1921 gaagtcagcg aacagttgcg cagccatttc ggggatttgc tttttgaaac cgtcatcccg
     1981 cgcaatatcc gccttgcgga agcaccaagc cacggtatgc cggttatggc ttatgatgcg
     2041 caggcaaagg gtaccaaggc gtatcttgcc ttggcggacg agctgatggc gagggtgtcg
     2101 gggaaatagg tcaatccaaa tcaggctgcc cgtgccttta tgccgtttga ccgagtgcgt
     2161 taatatggtg gattaaaata aaaatactta tatcgtcat
//
LOCUS       DAWFIE010000140         2184 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWFIE010000140 DAWFIE010000000
VERSION     DAWFIE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.141"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..510
                     /gene="mafB"
                     /locus_tag="WGQ71_002011"
     CDS             <1..510
                     /gene="mafB"
                     /locus_tag="WGQ71_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0707947.1"
                     /translation="KPGKAAVSGDFSAAYNTRTTRKVTTETEGLNRIRQNQKNSNIHE
                     KNYGRDNPNHINVLSGNSIQHILYGDEAGGGHLFPGKPGKTTFPQHWSASKITHEISD
                     IVTSPKTQWYAQTGTGGKYIAKGRPARWVSYETRDGIRIRTVYEPATGKVVTAFPDRT
                     SNPKYNPVK"
     gene            525..755
                     /locus_tag="WGQ71_002012"
     CDS             525..755
                     /locus_tag="WGQ71_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0707948.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            904..1641
                     /locus_tag="WGQ71_002013"
     CDS             904..1641
                     /locus_tag="WGQ71_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0707949.1"
                     /translation="MVKTADGYKAIAHIRAGESVLSKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLLSESGKTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPYGGSNNLEKAKLRAERLSKND
                     RAGKDFTKAGKEAVIDLNRIQNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVI
                     PKSKGGQGTPKNGQVLCRGCNIKKSNK"
     gene            1638..2057
                     /locus_tag="WGQ71_002014"
     CDS             1638..2057
                     /locus_tag="WGQ71_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HEZ0707950.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
BASE COUNT          781 a          406 c          438 g          559 t
ORIGIN      
        1 aaaccgggga aagctgcggt tagcggggat ttttctgctg catacaatac aagaacaact
       61 agaaaagtta ctacagaaac agaggggtta aatagaatca gacagaacca gaaaaatagt
      121 aatatacatg agaaaaatta tggaagagat aatcctaatc atattaatgt tttatctgga
      181 aattctatac aacatatact gtatggagat gaagcaggag gtgggcatct ttttcctggc
      241 aaacctggta agacaacatt cccccaacat tggtcagcca gtaaaataac tcatgaaatt
      301 agtgatatcg ttacatcccc aaaaacgcaa tggtatgcac agactggaac aggcggcaaa
      361 tatattgcta aaggaagacc agccaggtgg gtatcatatg aaacgagaga tggaattcgt
      421 atcagaacag tttatgaacc tgcaacagga aaagtggtaa ctgcattccc cgatagaacc
      481 tctaatccca aatataaccc tgtaaaataa tgagtatttg aaagatgaaa acattagacg
      541 aacgaataaa aaatttagga aaatctcttg aagatagaat tgacgctaat ctaattgatg
      601 ccgcactaga gtacattact ttttcagaac gtttgctagc ttttgaaacg ctatgtgatt
      661 atatagaaga tttcaatatc cagcttacgg aaaaagaatc ccaagaaatc tcttttatta
      721 ataaggaatt tggaattgaa agtacatcag attaataaat attaggtcgg tttaaaaacc
      781 caacaatcaa aaagtcgtct gaaactgtgt ttaatttaca gacgaccttt ccttcatttg
      841 aagcaggata ttgagaacta agttcttcaa aaatcctaca cctactcctt ccacggcagc
      901 accttggtca aaacggcaga cggctacaaa gccattgccc atatccgagc cggcgagagc
      961 gtcctctcca aggacgaggc aagtggaaaa acgggataca aacccgttac cgcccgatac
     1021 ggcaatccct atcaagaaac cgtttacatt gaaatttcag acggcatcgg caacagtcaa
     1081 accctgattt ccaaccgcat ccatccgttt tattcggacg gcaaatggat taaagcggaa
     1141 gatttgaaag cgggaagccg gctgttatcc gaaagcggca aaacccaaac cgtccgcaac
     1201 atcgttgtca aaccaaaacc gctcaaagcc tacaatctga ccgttgccga ttggcatacc
     1261 tactttgtca aaggcgataa ggcggaaacg gaaggggttt gggttcataa tgattgtccg
     1321 tatggcggtt caaataattt agagaaagca aaacttcgcg cagaacggct tagtaaaaac
     1381 gatcgggcag gaaaagactt tactaaagca ggaaaagaag ccgttattga tttgaatagg
     1441 attcaaaata atggtcaagt taagtgcgct aattgcggta ttgaaacaat tccagccaaa
     1501 caatctataa aaaatatttc tcctacttca aatgaaagac aagtagatca tgtcatccct
     1561 aaatctaagg gtggtcaagg tacacctaaa aacgggcagg tattatgcag aggctgcaac
     1621 attaagaaga gtaacaaatg aaatcgaaac ttactgtcgt ctattacgat ttggaaagca
     1681 atattgcaga agaaatactg tcaggaaaca taatgcctga tggaaatttt cttattcaag
     1741 agattcctct tttcgcaccg aatttggctt taaacgacat tgttgccata gaacgtgagg
     1801 ataagatgct gtttttcgac cacttgataa aagcttcagg aaataccacg ataaacatcg
     1861 ttgttttgga tcatttccca aaggatttat tggcagccat agaagaacac agtggtaaaa
     1921 tcagaaaaaa tggagagaat tatttatcgg ttaattttcc gcccaaaaaa tataattctg
     1981 atttaaaagg aattttaaat agatatgagg aagcaaatat cctcagctac agggaagctt
     2041 gtttgggctt ctcttaatcg atctataagt aagaatggat tacgttagat tgtaaaccta
     2101 acgattaaaa ggtcgtctga aaccgtgttt aattttcaga cgacctattt ccttcatttg
     2161 aaacaggata ttgggaatta agtt
//
LOCUS       DAWFIE010000141         2141 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWFIE010000141 DAWFIE010000000
VERSION     DAWFIE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.142"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..686
                     /locus_tag="WGQ71_002015"
     CDS             <1..686
                     /locus_tag="WGQ71_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEZ0707951.1"
                     /translation="FHHISTDEVYGDLGGTDDLFTETAPYAPSSPYSASKASSDHLVR
                     AWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVE
                     DHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYEDLI
                     TFVQDRPGHDVRYAIDTAKISRELGWKPQETFESGLHKTVQWYLDNKTWWQNVLNGSY
                     RLERLGTGK"
     gene            749..1615
                     /gene="rfbA"
                     /locus_tag="WGQ71_002016"
     CDS             749..1615
                     /gene="rfbA"
                     /locus_tag="WGQ71_002016"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ0707952.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     RIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQIKPSARGELEITDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            1669..>2141
                     /gene="rfbC"
                     /locus_tag="WGQ71_002017"
     CDS             1669..>2141
                     /gene="rfbC"
                     /locus_tag="WGQ71_002017"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0707953.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMRKDSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIGWPL"
BASE COUNT          558 a          599 c          527 g          457 t
ORIGIN      
        1 gtttccacca tatttccacc gatgaagtct atggcgattt aggcggcacg gacgatttgt
       61 ttaccgaaac cgcgccctac gcgccgtcca gcccctactc tgcctctaaa gcgtccagcg
      121 accacctcgt ccgcgcgtgg ttgcgtactt acggcttgcc gaccattgta accaactgct
      181 ccaacaacta cggtccttac cattttccgg aaaaactcat tcctttgatg attctgaacg
      241 cgcttgacgg caaaccgctg cctgtgtacg gcgacggtat gcaaatccgc gactggctgt
      301 ttgtcgaaga ccacgcgcgc gcactgtatc aggttgttac cgaaggtgtt gtcggcgaaa
      361 cctacaatat cggcggccac aatgaaaaag ccaatatcga agtggtcaaa accatctgcg
      421 ccctgctgga agaactcgct cccgaaaaac cggccggtgt ggcgcgttat gaagatttga
      481 ttactttcgt acaagaccgt cccggccatg acgtacgcta cgccattgat acagccaaaa
      541 tcagtaggga actaggctgg aaaccgcagg aaaccttcga gtccggcctc cacaaaaccg
      601 tgcaatggta tctggacaac aaaacctggt ggcaaaacgt attgaacggc agctatcgtt
      661 tggaacgctt aggtacaggc aaatagtttt cagacggcat cccgacgcaa tgccgtctga
      721 aaatccatcg caaaggaaga aagaaaagat gaaaggcatc atactggcag gcggcagcgg
      781 cacgcgcctc taccccatca cgcgcggcgt atccaaacag ctcctgcccg tgtacgacaa
      841 acccatgatt tactatcccc tgtccgtatt gatgctggca agaatccgcg atattttggt
      901 gattaccgcg cctgaagaca acgcctcttt caaacgcctg cttggcgacg gcagcgattt
      961 cggcatttcc atcagttatg ccgtgcaacc cagtccggac ggcttggcac aggcatttat
     1021 catcggcgaa gaatttatcg gcaacgacaa tgtttgcttg gttttgggcg acaatatttt
     1081 ttacggtcag tcgtttacgc aaacattgaa acaggcggca gcgcaaacgc acggcgcaac
     1141 cgtgtttgct tatcaggtca aaaaccccga acgtttcggc gtggttgaat ttaacgaaaa
     1201 cttccgcgcc gtttccatcg aagaaaaacc gcaacggccc aaatccgatt gggcggtaac
     1261 cggcttgtac ttctacgaca accgtgccgt cgagttcgcc aaacaaatca aaccgtccgc
     1321 acgcggcgaa ttggaaatta ccgacctcaa ccggatgtat ttggaagacg gctcgctctc
     1381 cgttcaaata ttgggacgcg gtttcgcgtg gctggacacc ggcacccacg agagcctgca
     1441 cgaagccgct tcattcgtcc aaaccgtgca aaacatccaa aacctgcaca tcgcctgcct
     1501 cgaagaaatc gcttggcgca acggttggct ttccgatgaa aaactggaag aattggcgcg
     1561 cccgatggcg aaaaaccaat acggccaata tttgctgcgc ctgttgaaaa aataatgttt
     1621 gaggccgtct gaaacttttc agacggcctt tagatgaaag ataaaaagat gaacatcatt
     1681 gataccgcca ttcctgacgt aaaactgctt gagccccaag tcttcggcga cgcgcgcggc
     1741 ttttttatgg aaaccttccg agacgagtgg tttaaaaccc aagtctgcga acgcactttc
     1801 gtgcaggaaa accactccaa atccggcaaa ggcgtattgc gcggcctgca ctaccaaacc
     1861 gaaaacacac aaggcaaact cgtacgcgta gttgtcggcg aagtattcga cgtggccgtc
     1921 gatatgcgta aagactcccc cactttcggc aaatgggcgg gcgcaaccct gtccgcgcaa
     1981 aaccaatgcc agctttggat acccgaaggc ttcgcgcacg ggttttgcgt tttgggcgac
     2041 gcagcggaag tcgtgtacaa atgcacggac tattacaagc ctgaagccga acaggtttta
     2101 atatggaacg acccgacagt cggcataggc tggccgcttc a
//
LOCUS       DAWFIE010000142         2108 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWFIE010000142 DAWFIE010000000
VERSION     DAWFIE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.143"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(383..793)
                     /locus_tag="WGQ71_002018"
     CDS             complement(383..793)
                     /locus_tag="WGQ71_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707954.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIEGTDFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(1381..1764)
                     /locus_tag="WGQ71_002019"
     CDS             complement(1381..1764)
                     /locus_tag="WGQ71_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707955.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            complement(1730..>2108)
                     /gene="mafB"
                     /locus_tag="WGQ71_002020"
     CDS             complement(1730..>2108)
                     /gene="mafB"
                     /locus_tag="WGQ71_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ0707956.1"
                     /translation="MQTVDGEMAGGNKPPKPSTQQHSTHSDNNIGLPAPYVKPDTSIS
                     PTGTIYVDSKGNAIPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHHANTHQDPR
                     ALTGHAHVPGVTNDDGTPWLPIK"
BASE COUNT          620 a          377 c          349 g          762 t
ORIGIN      
        1 ctctacggat ggggcggagt tacccgtatg attgccgtat ggccggatac ttgtatccga
       61 caaaaatatt tgacgcatct atcgtttccg aaatgccgct gttgaaaaat gtcggattcg
      121 agaatccgac ctacggttgc tgaacaatgt tttgtagaac ttgctcaaaa tccatgtttc
      181 gccattttcc tttatatata cctaatacag cacgggttac gatttcgttt aagtcataaa
      241 tagccattta cgtcctctgc taattttcag cgagtagatt gggttaccaa acccaacaaa
      301 aaagcagcct gaaaaatttc aggatgcttg ttttatgttt tttatcaatg aattaggttt
      361 taatccagcc tgcgcttgct agttaaacag tatttacata aaaatcttgt actgaacaaa
      421 caagcgtatc atgttcatca tgaaatgatg ggcataacgt taatctacaa atgtttacat
      481 ttaaagatat tttttcagtt tcaatatttt taatttgtaa ggttaataca tgaggatatt
      541 tgtgatatag ctgatttttt aattgttcgt tgtctaatat agcaaaaggt aattttgcac
      601 acaaataacc aataactttg tttttatgaa aaaaaactat ccaattttca attccttcat
      661 cccaatcaaa agtaaaatcc gttccttcta tgataaattt agcacaagat attaaatcta
      721 acttaaccca agtggattta tacagactca atgctgatga agttataata ttatctaaat
      781 tataaatttt cataaatcaa cgttccttac ctggtgttat atgcacaggt ctaatcccat
      841 ctttattcgg ctttctacga taaatgttaa ttatatgtgt cggttttccg tctgtatagc
      901 caactacgtc gttcgttgga acttttgcct tattttgact atcccataaa ttatgtttat
      961 ctgcatatct agcagcatct aatgtagctt tttccgcatc aacatctgat ttaaataaac
     1021 ttttgctatc tatcttgtct agactagatg catcaggttt gtgtcgtaaa aagcactgtc
     1081 ctgtaatagg ctcgcggcag caagttgtgc ttccggactt aattttccta aatcattaat
     1141 accttgtaaa gtttgctgcg cagagatgta ggtcggatat ttgtatccga caaaaatatt
     1201 tgacgcatct atcgtttccg aaatgccgct gttggaaaat gtcggattcg agaatctgac
     1261 ctacggctgc tgactatttt cttgtttata taattctaca actgcaaact actatcaaat
     1321 ctttttatct ctggctctac actcagcaag aatattttta ttttacaaaa cataggctag
     1381 ttatttgagt atattgtcaa attctaattt gtttttttga tagatttttt ttataccatt
     1441 aatacattca ttagttgtaa ttctgccaag cgaatgtaat ttatcaatat caaaacaatc
     1501 ggtataaacc ctattcacag ttttatcttg tatttcctta tgaatactta atatattgag
     1561 atagtttaga aattttattt tctcagattg aggtaactca tcaaaaccaa gccaataata
     1621 aaaattgtta tatgatttta aaaaatcaac aatttttatt tttcctaaaa ccaaatcttc
     1681 aactaactgg gttgcataaa taatatcatt tatgatttta acaggtaaat tatttgatgg
     1741 gtaaccatgg ggttccgtca tcatttgtaa ctcctggtac atgtgcgtgt cctgtaagtg
     1801 cgcgtgggtc ttgatgggta tttgcatgat gagcatcatc tattctggct ccagtcaagt
     1861 ttccattact atctcggact tggataaaac gagcatcagg cgaacctgtt atataaccgc
     1921 ctattggtgt tggtattgca tttcctttgc tgtctacata aattgttcct gtcggagaaa
     1981 tagatgtatc aggtttaaca tatggggcag gtaagcctat attgttatca gagtgtgtag
     2041 agtgttgctg cgtacttggt ttcggtggct tatttccccc agccatctcc ccgtctacag
     2101 tctgcata
//
LOCUS       DAWFIE010000143         1867 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWFIE010000143 DAWFIE010000000
VERSION     DAWFIE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.144"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            403..1512
                     /locus_tag="WGQ71_002021"
     CDS             403..1512
                     /locus_tag="WGQ71_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0707957.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCITARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIVIGRDKLFIHPRLMELLRGEGNSFTSF"
BASE COUNT          578 a          450 c          402 g          437 t
ORIGIN      
        1 aggaatgaca gtttaaaagt tacccgaaac ctcaaaaaaa aggctgtgtt ttaatatagt
       61 gttgatagac gtacttggct tccatgtatt caatcgtgga aatctatatc ttcgtcctcg
      121 ccgaaatagt ctatgcccga tatacaattt tgatacacaa acttggaaat atgggtatcg
      181 tcgccggagc gatagaatgc ggacagttat atatatacgg tttttttagg gaagcgggca
      241 gatgaagggc ggattttgaa ttaaccccat gcaaattgac ttttgcaggc ggttttacct
      301 cccatatatt tacaaaagcc aaatttttaa acatatatcc ttgatatata cacggcgtaa
      361 acatatactg gaaacatctt taaattttcc gaaattttaa atatgagcaa ctggaaaccc
      421 gatattccct ataacgattt accacccctg ccgccaaaac aggatattga aagcaaaacc
      481 atcctgaaac gttgtataac cgcccgtgca tcccttgccc gtttaaagca ggcggcagaa
      541 ctgataccga atcaagccat gctgattaac acccttcctg ttatggaagc ccgtgcaagt
      601 tcggaaattg aaaacatcgt aaccacaacg gataagctgt ttcaatccct gcaaatggat
      661 acggaacggc aagaccctgc cacgaaagaa gccctgcaat accgcaccgc cctgtttgca
      721 ggctatgaat cactgacgag ccgcccttta tgcacacaaa ccgccatcat ggtctgcaac
      781 gccatcaagc acccctacga aatggccatc cgcaaaacag gcggcacagc cctaaaagga
      841 ggcaacagcg gaaatgttgt ctataccccg cccgaaggag aagaaaccat acgcggcaag
      901 ctggcaaatt gggagcggtt tattcacgaa agcggcgatt tagacccgct tatcatcatg
      961 gcggcggcac attaccaatt tgaagccatc catccgttta cggacggcaa cgggcggacg
     1021 gggcgcatat tgaacagcct gctattgatt gaaaaagggc ttttggattt gcctattttg
     1081 tatttgagcc gctacatcat cgaaaacagg gcggactatt accgcctgct gttaggcgta
     1141 accgaacggc aggactggga aagctggata atctacatct tagacggcgt agccgacacc
     1201 gccgattgga cggtatcgaa aatagatgcg atacgccgcc tgttcgagca gacacggcaa
     1261 cacatacgga cacacgcaca aggaatctac acgcacgaac tggtaaatct tctgtttgag
     1321 cagccatata cacgcattgc caacctagaa gcggcaggga tagccaaacg gcagacggcc
     1381 tctaagtacc tgaaagagct ttcagacata ggtgtgctgc aagaaatcgt catcggcagg
     1441 gacaaactat tcattcatcc gcgcctaatg gaactattgc ggggagaggg caacagcttt
     1501 acttcattct aaccccctct tcccccccca catgactaac acgaaacagg gattttgaca
     1561 cccgaaccga gaccccttgt atttcccccg cgaaaagccg gcatccgccc gcgtatcatg
     1621 ggagcaacaa aacccctgcc taaaattttg acttgtgcaa attgggggta tatttggggg
     1681 tatattgaaa atggctaaaa taaaatgttt aataaacaaa atgttgaacc ttaatttcga
     1741 tagagcatct gcatatcgta ttgaggcgtt catggaattt gagaaagcta tttttaaata
     1801 agaaaaggta actattaaat agctaccttt ctaaaattaa atatcaacac atcgttaaaa
     1861 cacagcc
//
LOCUS       DAWFIE010000144         1816 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWFIE010000144 DAWFIE010000000
VERSION     DAWFIE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.145"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            58..858
                     /locus_tag="WGQ71_002022"
     CDS             58..858
                     /locus_tag="WGQ71_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0707958.1"
                     /translation="MDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNN
                     PESATQYSYPAYDTTATTKADALSSVTTSTSLLNAPAAALTKNSGRKGERSAGLSVNG
                     TGDYRNETLLANPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRT
                     ELHLYNAETLKAQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTV
                     KASDRLMVDFSDITPYGDTTAQNRPDFKQNNGKNPDAGNEVIRRRKGG"
     gene            862..>1816
                     /gene="mafB"
                     /locus_tag="WGQ71_002023"
     CDS             862..>1816
                     /gene="mafB"
                     /locus_tag="WGQ71_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0707959.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDEGFTVYRLNWEGHEHHPADGYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYSNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKF
                     AVIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEAVFNVAA"
BASE COUNT          473 a          660 c          423 g          260 t
ORIGIN      
        1 cgctttgccg tcgaacaaga actcgtcgcc gcatcgtccc gcgccgccgt caaagaaatg
       61 gatttgtccg ccctgaaagg acgcaaagcc gccctttacg tctccgttat gggcgaccaa
      121 ggttcgggca acataagcgg cggacgctac tctatcgacg cactgatacg cggcggctac
      181 cacaacaacc ccgaaagtgc cacccaatac agctaccccg cctatgacac taccgccacc
      241 accaaagccg acgcgctctc cagcgtaacc acttccacat cgcttttgaa cgcccccgcc
      301 gccgccctga cgaaaaacag cggacgcaaa ggcgaacgct ccgccggact gtccgtcaac
      361 ggcacgggcg actaccgcaa cgaaaccctg ctcgccaacc cccgcgacgt ttccttcctg
      421 accaacctca tccaaaccgt cttctacctg cgcggcatcg aagtcgtacc gcccgaatac
      481 gccgacaccg acgtattcgt aaccgtcgac gtattcggca ccgtccgcag ccgtaccgaa
      541 ctgcacctct acaacgccga aacccttaaa gcccaaacca agctcgaata tttcgccgtc
      601 gaccgcgaca gccggaaact gctgattgcc cctaaaaccg ccgcctacga atcccaatac
      661 caagaacaat acgccctctg gatgggacct tacagcgtcg gcaaaaccgt caaagcctca
      721 gaccgcctga tggtcgattt ctccgacatc accccctacg gcgacacaac cgcccaaaac
      781 cgtcccgact tcaaacaaaa caacggtaaa aaccccgatg ccggcaacga agtcatccgc
      841 cgccgcaaag gaggataaac cgtgaaaccg ctgcgcagac tgacaaacct ccttgccgcc
      901 tgcgccgtag cggccgccgc cttcggacag cccgccctcg cagcggactt ggcgcaagac
      961 ccgttcatta ccgataacgc ccaacggcag cactacgaac ccggaggcaa ataccacctc
     1021 ttcggcgacc cgcgcggcag cgtctccgac cgcaccggtc aaatcaacgt catccaagac
     1081 tatacccacc agatgggcaa cctgctcatc caacaggcaa acatcaacgg cacaatcggc
     1141 taccacaccc gcttttccgg acacggacac gaagaacacg cccccttcga caaccacgcc
     1201 gccgacagcg caagcaaaga acaaggcaac gttgacgaag gctttaccgt gtaccggctc
     1261 aactgggaag gacacgaaca tcatcccgcc gacggctatg acggcccgaa gggcggcaat
     1321 taccccaaac ctacgggcgc acgcgacgaa tacacctatc acgtcaacgg cacagcacgc
     1381 agcatcaaac tcaatccgac cgacacccgc agcatccggc aacgcatatc cgacaactac
     1441 agcaacctcg gcagcaattt ctccgaccgc gccgatgaag ccaacagaaa aatgttcgag
     1501 cacaatgcca agctcgaccg ctggggcaac agcatggagt ttatcaacgg cgtcgccgcc
     1561 ggcgcgctca acccctttat cagcgcgggc gaagccttgg gcataggcga catactgtac
     1621 ggaacgcgct atgccataga caaagccgca atgcgcaaca tcgccccctt gcccgccgag
     1681 ggcaaattcg ccgtcatcgg cggcttgggc agtgtggcgg gctttgaaaa gaatacgcgc
     1741 gaagccgttg accggtggat acaggaaaat cccaatgccg ccgaaaccgt cgaagccgtc
     1801 ttcaacgttg ccgcag
//
LOCUS       DAWFIE010000145         1784 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWFIE010000145 DAWFIE010000000
VERSION     DAWFIE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.146"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(150..1139)
                     /gene="porB"
                     /locus_tag="WGQ71_002024"
     CDS             complement(150..1139)
                     /gene="porB"
                     /locus_tag="WGQ71_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ0707960.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVAHNGAQA
                     ASVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHQDVDNVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVEDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFDD
                     ADYTNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGESKFVSTAGGVGLRHKF"
BASE COUNT          449 a          412 c          457 g          466 t
ORIGIN      
        1 gggaatgacg gcatattttg acattgaata aaaaagacta aaaacaggaa aaagccaaac
       61 agaaaacagc caaacagaaa acaggaaaaa gccaaacaga aaaaagcctg tctggcgaca
      121 ggctttttgt tgataccaat ctttgcagat tagaatttgt ggcgcagacc gacaccgccg
      181 gcagtcgata cgaatttgct ttcgcctttg ccttcttgca accaaccggc agaaaccaag
      241 gcagaagtgc gtttggagaa gtcgtattcc gcaccgacaa ccacttggtc gtaatcgttg
      301 gtgtagtctg catcatcaaa cgagcctttg aagccgtggg cgtaagaaac gcggggcgtt
      361 acgttgccga agcggtatgc caaggtagcg gcaacttcgg tttgagagtt gtgcgaattg
      421 tcttcaacca gtttcgcgtc ttgttgctgt acggctacgg aagcgtatag ggcatcattg
      481 tcgtaaccgc tgaccaaacg gtgaatctgg tatttctcaa tcttcacgtt atccacatcc
      541 tgatgtcttt tataggcacc gccatattgc acgaagaagc cgccgttttt gtagttgaag
      601 ccggcatggt aagattcgct gttatgtctg cctgcattgt cgttaagcgc gtattgtacg
      661 ctgccgctga ggccggcaaa ttcgggagaa tcgtagcgta cggaaatgag gcgtgcttcg
      721 ggttcggcaa ttttgtttac acccaaatag tcgcttttgc tatcccaagg attgatgtcg
      781 ccggtgtctt tcaggacgct gttcaaacgg ccgacgcgca atttaccgaa gccgcctttc
      841 aaaccgatga aggattggcg gttgccccaa ccggagtcag taccggcgat agatgccttt
      901 tgctcaacct gccaaatggc tttcaggccg ttgccgaggt cttcttggcc tttgaagccg
      961 attttcgaac ccaaatcaac gatgccggta ccggtttcaa cgctagccgc ctgagctcca
     1021 ttgtgagcta cggagcggga agtttctacg ccggctttga tggtgccgta cagggtaacg
     1081 tcagccattg ctgcaacagg aagggctgcc aaagtcaggg caatcaggga ttttttcatt
     1141 gctgtattcc ttttttggtt aagaaattta agcaggccgg gctttccaag ccgcttagct
     1201 ttgcatttac cgccgacgtt ttcggcgatt ccatgtctga aactatagat gttcctgtct
     1261 gaaaaaacaa acttctgcct gtttttgtgt ggaattcgat gtgtattttg aagggcggat
     1321 agagatattg tgggtatttt ttgttttata acatatggtt atttaaattt tttaagattt
     1381 gcatttttac aacacttact cgggagggta ttggagggca ttgcaaaccg agggttataa
     1441 agacggcaaa aaacgcgcac gtggtttctt tcggaacggg cggatgcaaa acccgcccct
     1501 gcggcaggag atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac
     1561 aaataggacg gaaccgattc acttggtgct tcagcacctt agagaatcgt tctctttgag
     1621 ctaaggcgag gcaacgctaa ccgtcattcc cgccactttt cgtcattccc gctcaggcgg
     1681 gaatctagaa tctcggactt tcagataatc tttgaatatt gccgctgcct taaggtctgg
     1741 attcccgcct gcgcgggaat gacgaatcca tccgcacgga aacc
//
LOCUS       DAWFIE010000146         1718 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWFIE010000146 DAWFIE010000000
VERSION     DAWFIE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.147"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(195..1394)
                     /locus_tag="WGQ71_002025"
     CDS             complement(195..1394)
                     /locus_tag="WGQ71_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ0707961.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNFQLQLTEQP
                     QTANTQQGGKVKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGG
                     GATRWGNRESFVGLAGEFGTLRTGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDD
                     MPVSVRYDSPDFSGFSGSVQFVPIQNSKSAYTPAHWNTVYNTNGTTTTTTTFVPAVVG
                     KPGSDVYYAGLNYKNGGFAGNYAFKYARHANVGRDAFELFLIGSATSDQAKGTDPLKN
                     HQVHRLTGGYEEGGLNLALAAQLDLSENGDKAKTKNSTTEIAATASYRFGNAVPRISY
                     AHGFDLIERGKKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAAS
                     VGLRHKF"
BASE COUNT          445 a          431 c          402 g          440 t
ORIGIN      
        1 tatatgtgca aaccaagccg aaaatgcgaa ataccgtctg aaaatctttc agacggtatt
       61 tgctgtcttt attgccgttt ttcttccgta tccggatttt tgtttggggc tgaagcagat
      121 tggcagtcag attgcaatca aagaatgaaa gcaagccgtc aaaaacaaag ctatccgctt
      181 caccgccccg atatttagaa tttgtggcgc aaaccgacgg aggcggcatt aatttgagtg
      241 tagttgccga tgccggtatt gcgtttcagc caagcgccag acacgatggc ggaagtgcgt
      301 ttggaaaaat cataatcaac gccggcgatg atttgatcgt agctggtatt ttcgcctttt
      361 ttaccgcgtt cgatcaagtc aaaaccatgg gcatagctga tgcgcggaac tgcattaccg
      421 aagcggtagg aagcagtggc ggcaatttcg gtcgtactgt ttttggtttt ggctttgtcg
      481 ccattttcag acaaatccaa ctgagccgcc aaggcgagat tcaagccgcc ttcctcatag
      541 ccgcccgtca ggcggtgtac ctgatggttt ttcaagggat cggtaccttt ggcttgatca
      601 ctcgtcgcgc tgccgattaa gaacaactca aaagcatcac gtccgacatt ggcgtgtctc
      661 gcatatttaa aggcatagtt cccggcaaaa ccgccatttt tgtaattcag accggcataa
      721 tacacatccg atccgggctt gccgacaaca gccggaacga aagtagtagt agtagtagta
      781 gtaccgttag tgttatacac agtattccaa tgagccggcg tataggcgga cttgctgttt
      841 tggatcggaa cgaattgaac gctgccgctg aaaccggaaa agtccggaga gtcgtagcgt
      901 acggaaaccg gcatatcgtc gtggcgtttg aaaataccca attgcgaagc cacatcatta
      961 ttgctgtccc aaggatcaat ggcttggctg gcatcgtcaa actgatttgc aacgcggccg
     1021 gtgcgcagcg tgccgaattc gcctgccaag ccgacaaagg attccctatt gccccaacgg
     1081 gtcgcgccac cgccggcaac ggatacgtct tgctcaagct gccaaacagc tttcagacct
     1141 tcgcccaaat cctcgctccc cttaaagccg ataaacgagc cgaaatcact gattttcgtc
     1201 ctgatgcggc ttttggcctt agtaactttt accttaccgc cttgctgagt gttagcggtt
     1261 tggggctgct cggtcaattg cagctggaag ttcctgcctt ccacgccggc tttgatttcg
     1321 ccgtacaggc tgacatcggc aacggccgca agcggcagtg cggacaatac gagggcggta
     1381 agtttttttc gcatatcggc ttccttttgt aaatttgata aaaacctaaa aacatcgggc
     1441 aaacacccga tatgtcttca attatacccc cccccccgca aaaaaccatt tttcagaaca
     1501 aatatctgat aaatgccgca acctttattt taaaaatgat tatattttaa tataaaacaa
     1561 tagcttattt tttcaaaaac cgttgtgttt ctacaacaca attcaagcgc agacctcgtg
     1621 cgggccgatg cgctgctgcc cggatgcagt ctcggctttt taaaacgcca taaaaaaaca
     1681 cacgcggcac tttatagtgg atgaacaaaa accagtac
//
LOCUS       DAWFIE010000147         1446 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWFIE010000147 DAWFIE010000000
VERSION     DAWFIE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.148"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            32..1369
                     /locus_tag="WGQ71_002026"
     CDS             32..1369
                     /locus_tag="WGQ71_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0707962.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIIGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT          264 a          349 c          380 g          453 t
ORIGIN      
        1 tatactacct tcacatttct taataaattt tatgagtaac catacttctt ggtcgtccaa
       61 aatcggtttc gtccttgctg cggcaggttc ggccatcggt ttgggcgcga tttggaaatt
      121 tccttatacg gcaggcacca acggcggcgc ggtgtttttc ctgctgtttt tgatatttac
      181 tatcttggtc gccctacccg ttcagcttgc cgaattttat atcgggcgca cgggcggtaa
      241 aaatgccgtc gattccttca gggttctgcg tccgggcacg caatggcttt gggtcgggcg
      301 tatgggcgtt gccgcctgct ttattttgct gtcgttttac agcgtggtcg gcggatgggt
      361 attaaattat gtcgtccaca gttttacggg ggcggttcat accggcgcgg actttgaagc
      421 cttgttcggc gcgacgattt ccaatccggc aggttcgctg tcctatcagg cactgtttat
      481 gctgattacg gtttgggtgg tcaaaggcgg catttcagac ggcattgaaa aggcaaaccg
      541 ttatctgatg ccggggctgt ttatcctctt tattgcgctg gcaatccgtt cgctgacgct
      601 gccgggtgca atggagggcg tgtctttcct gctcaaaccg aattggtcgt actttaaagc
      661 cgatacgatg attacggctt taggccaggc gttttttgcc ctgagcatcg gcgtttccgc
      721 catgattacc tacgcttcat atttgggaaa agatcaggat atgttccgtt ccggccatac
      781 gattatgtgg atgaacctct tggtttcgct gcttgccggc ctggtgattt ttccggcggt
      841 gttcgccttc ggttttgaac cgagccaggg gccgggattg atttttatcg tattgcccgc
      901 agtgtttatg aagatgccgt tcggtacggt tttgtttgcg gtatttatgc tgctggtcgt
      961 tttcgccacg ctgacttcgg cattttcgat gttggaaacg gtcattgcct caaccatccg
     1021 ccaagacgag cgcaaacgca aaaaacacac ttggcttatc ggcacggcca ttttcattat
     1081 cggcatcccg tccgcgctgt ctttcggcgt atggggcgag tttaaggttt tcggcaaaac
     1141 catttttgat ttgtgggact atgttatttc cgccgtcatt atgccgattg gtgctttgag
     1201 tgtttccatc tttaccgcct ggattcagga caagcagtct gtgttaaaag atgccggcgc
     1261 gggcagcacc gtaccacggg cagtgctgct gctgtggctg aataccttgc gctaccttgc
     1321 cccgattgcc attattattg ttttcatcaa ttctttggac atcctttaaa agccatccaa
     1381 acagcaaaaa tgccgtctga aagcctttca gacggcattt ttgcttcggg ttcagcctat
     1441 ttcgtt
//
LOCUS       DAWFIE010000148         1284 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWFIE010000148 DAWFIE010000000
VERSION     DAWFIE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.149"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..>1284
                     /locus_tag="WGQ71_002027"
     CDS             <1..>1284
                     /locus_tag="WGQ71_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0707963.1"
                     /translation="TKLVDGSFAKHGYAALAELDSNGDNIINAADAAFQTLRVWQDLN
                     QDGISQANELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGSYTKTDGTTAKMGDLL
                     LAADNLHSRFKDKVELTAEQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSAAETK
                     EAQLALLDNLIHKWAETDSNWGKKSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVS
                     LSDKAKAAIDAARDRIAVLDAYTGQDSSTLYYMSEEDALNIVKVTNDTYDHLAKNIYQ
                     NLLFQTRLQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEMLAYGE
                     LRSWYEGRRLMADYVEEAKKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNVGFGH
                     NKNVSLYGNDGNDTLIGGAGNDYLEGGSGSDTYVFG"
BASE COUNT          385 a          291 c          299 g          309 t
ORIGIN      
        1 accaaactgg tagacggttc ttttgccaaa cacggctacg cggctttggc cgaattggat
       61 tcaaacggcg acaacatcat caacgcggca gacgccgcat tccaaaccct gcgtgtatgg
      121 caggatctca atcaggacgg catttcccaa gctaatgaat tgcgtaccct tgaagaattg
      181 ggtatccaat ctttggatct cgcctataaa gatgtaaata aaaatctcgg taacggtaac
      241 actttggctc agcaaggcag ctataccaaa acagacggta caaccgcaaa aatgggggat
      301 ttacttttag cagccgacaa tctgcacagc cgcttcaaag acaaagtgga actcactgcc
      361 gaacaggcaa aagccgccaa tcttgcgggc attggtcgtc tgcgcgattt gcgcgaagct
      421 gccgcattgt ccggcgattt ggccaatatg ctgaaagctt attctgccgc cgaaactaaa
      481 gaagcacagt tggcattgtt agataatttg attcacaaat gggcggaaac cgattcgaac
      541 tggggcaaaa aatcgccaat gcgactttca accgattgga cgcaaacggc taatgaaggt
      601 attgcactga caccatccca agtagcacaa ctaaaaaaga acgctttagt ttccctttct
      661 gataaagcta aagcagctat tgacgccgcc cgcgaccgca ttgccgtgct tgatgcctac
      721 acggggcagg attccagcac actctattac atgagcgagg aagatgcgct taatatcgtc
      781 aaagtaacca acgatacata cgaccatctc gccaaaaaca tctaccaaaa cctgttgttc
      841 caaacccgtt tgcagccata tttgaatcaa atcagtttca aaatggaaaa tgatacgttc
      901 actttggatt ttagtggtct tgttcaagca tttaaccatg tcaaagaaac taatccgcaa
      961 aaagcttttg tggatttggc cgagatgctt gcatatggcg aacttcgttc ttggtatgaa
     1021 ggccgaagac taatggccga ttatgtggag gaggcaaaaa aagcaggtaa atttgaagat
     1081 taccagaaag tgttgggtca ggagaccgtt gcattattag ctaaaacatc gggtacgcaa
     1141 gcagatgata tcctgcaaaa tgtaggcttt ggtcataata aaaatgtttc tttatatggt
     1201 aatgacggca acgacactct gatcggcggt gccggtaatg attacttgga gggcggcagc
     1261 ggttcggata cttatgtctt cggc
//
LOCUS       DAWFIE010000149         1211 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWFIE010000149 DAWFIE010000000
VERSION     DAWFIE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.150"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..218)
                     /locus_tag="WGQ71_002028"
     CDS             complement(<1..218)
                     /locus_tag="WGQ71_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
                     /protein_id="HEZ0707964.1"
                     /translation="MVKTADGYKAIAHIQTGEHVFAKDETSGKTGYKPVTTRYGNPYQ
                     ETVYIEVSDGIGNSQTLISNRIHPFYSDG"
     gene            complement(433..792)
                     /locus_tag="WGQ71_002029"
     CDS             complement(433..792)
                     /locus_tag="WGQ71_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707965.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKSGWVFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDN
                     YKVNWKINNGNLIQTTN"
     gene            complement(795..1151)
                     /locus_tag="WGQ71_002030"
     CDS             complement(795..1151)
                     /locus_tag="WGQ71_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707966.1"
                     /translation="MSDSARQLYQNAKYREALDIHYEDLIRRKTDGSSKFINGREIDA
                     VTNDALIQAKRTISAIDKPKNFLNQKNRKQIKATIEAANQQGKRAEFWFKYGVHSQVK
                     SYIESKGGIVKTGLGD"
BASE COUNT          332 a          225 c          199 g          455 t
ORIGIN      
        1 ccatccgaat aaaacggatg gatgcggttg gaaatcaggg tttggctgtt gccgatgccg
       61 tctgaaactt caatgtaaac ggtttcctgg tacggattgc cgtatcgggt ggtaacgggt
      121 ttgtatccag tttttccact ggtttcatct ttggcaaaga cgtgctctcc agtttggata
      181 tgtgcgatgg ctttgtagcc gtctgccgtt ttgaccaagg tgctgccgtg gaaggagcag
      241 gtgtaggatt tttaaagaac ttagttctca atatcctgtt tcattcatca aaatgccgtc
      301 tgaaagctga ataccgcttc agacggcatt ttggtggttg ggtttttaag ccaacctacg
      361 cttactgaaa accaaattga gtttcagaca gtttttaggt ttgggtgtcc aatctaactt
      421 atatttgtcc atttaattag tcgtttgtat caaatttcca ttatttattt tccaatttac
      481 tttataatta tcttcataat aatctaattc aaaaaaacct gatatttcaa tatccaattc
      541 cattattgtt ttaatacatt tttcaaaata aataatgaaa taagatttta cgcatgcacc
      601 aaaaaaaata tagctgctcc aattaaaact atttgtcggg aaaacccacc cgcttttata
      661 tatttttgca gattctttct cttcgatatt aaagggacaa ttattccaaa aattattaat
      721 gttttcttgg atgattttta tatcatcgtc agagcattca atccatcctt ttatggaaac
      781 atatgatgcc atgtttaatc tcctaaacct gttttaacaa tgccgccttt tgattcaata
      841 tatgacttaa cttgtgaatg aacaccgtat ttaaaccaaa attctgcacg ttttccctgt
      901 tggtttgctg cttcgatggt tgctttaatt tgctttctat ttttttgatt taagaaattt
      961 ttaggtttat ctattgctga aattgttctt ttggcttgta ttaaagcatc attcgtaaca
     1021 gcgtcaattt ctctgccgtt aataaatttt gatgaaccat cagtttttct tctaattaaa
     1081 tcttcataat gtatatctag agcttctcta tactttgcat tttgatataa ctgtctcgca
     1141 ctatcagaca aagccaattt ctttttataa gaatcagcaa aatccccgct aaccgcagcc
     1201 ttccctggtt t
//
LOCUS       DAWFIE010000150         1210 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWFIE010000150 DAWFIE010000000
VERSION     DAWFIE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.151"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            54..578
                     /locus_tag="WGQ71_002031"
     CDS             54..578
                     /locus_tag="WGQ71_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0707967.1"
                     /translation="MSRLDTIRAVHLMLDIFKKMVDRDMPVRLNVAGIGEEMDNLKAQ
                     AERLGIDGKVTFLGGVRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILEAGLYDTPVV
                     TYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPELREAMGKRLHAHVAALCS
                     DDEIYRTTMAAYTM"
     gene            598..>1210
                     /gene="pglH"
                     /locus_tag="WGQ71_002032"
     CDS             598..>1210
                     /gene="pglH"
                     /locus_tag="WGQ71_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
                     /protein_id="HEZ0707968.1"
                     /translation="MNITIVAPYCSLPSEPYFNRFWYLAERLSQSHDVLLITSRFRHY
                     DKSFRRHEDAAATSNGRLRVKLLDEPGYRKNVSLARVASHRVFVRNLARWLHSPQAAE
                     QDIVYSAYPLMATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRCADALVAVSQTYLDRAKEANPNVPGEAVYIGTDFAA"
BASE COUNT          277 a          374 c          315 g          244 t
ORIGIN      
        1 ccccggaatt tcctttccgg aaaaccgaaa aagaatgcgc cgtactcggc acgctgtcgc
       61 gtctggacac catccgcgcc gtgcatctga tgctggacat tttcaaaaaa atggtcgacc
      121 gcgatatgcc cgtacgcctg aacgtggcgg gcataggcga agaaatggac aatttgaaag
      181 cgcaggcgga acgtctgggc atagacggca aagttacctt cctcggcggc gtgcgcgatt
      241 tgaccgccta cttcaaagac gtggatattt tggtgaacac gccccactgc atcggcgacc
      301 acggcgcagg cgtgggcaac aacatcttag aagccggact ttacgacaca cccgtcgtta
      361 cctacgatat ggcgggcatt tccgaaatgg tcgtcaacgg ggaaacgggt tactgcatcc
      421 ccttcggcga cgaagaagcc tttatcgccg ccgtagacac gctcgtcaga caaccggagt
      481 tgcgcgaagc aatgggcaaa aggctgcacg cacacgtcgc cgcactctgt tccgacgacg
      541 aaatctaccg taccaccatg gcggcctata ccatgtaatg ccgtctgaaa gccgccgatg
      601 aacatcacca tagtcgcccc ttattgctcg ctgccgtccg aaccgtattt caaccgcttt
      661 tggtatctgg cagaacgatt gtcgcaatcg cacgacgtgt tgctgattac cagccgtttc
      721 cgccactacg acaaatcatt cagacggcat gaagatgctg ccgccacttc aaacggcagg
      781 ctgcgcgtca agctgctgga cgaacctggc taccgaaaaa acgtctccct cgcccgcgtc
      841 gccagccacc gcgtattcgt ccgcaatttg gcacgctggc tgcacagccc gcaggcggcg
      901 gaacaggaca tcgtctattc tgcctatccg ctgatggcga ccaacctgct gttgggcaaa
      961 cacaaagcgc gtttgggcta caaactgatt gtcgatgtgc aggacgtatg gccggagtct
     1021 ttctcctcgg tcgtaccatt tttgaaaaaa gtaccgcaca agctgctgcc ctttgcttca
     1081 cgcgccaacc gtgcctaccg ctgcgccgac gcgctggttg ccgtatcgca gacttatctc
     1141 gaccgcgcca aagaagccaa tccgaacgta cccggcgaag ccgtctatat cggtacggat
     1201 tttgccgcca
//
LOCUS       DAWFIE010000151         1165 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWFIE010000151 DAWFIE010000000
VERSION     DAWFIE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.152"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            1..132
                     /locus_tag="WGQ71_002033"
                     /pseudo
     CDS             1..132
                     /locus_tag="WGQ71_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            234..395
                     /locus_tag="WGQ71_002034"
                     /pseudo
     CDS             234..395
                     /locus_tag="WGQ71_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            547..1116
                     /locus_tag="WGQ71_002035"
     CDS             547..1116
                     /locus_tag="WGQ71_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ0707969.1"
                     /translation="MLFPRIIAASAQRKLSVYLKKGGLVAYPTESCYGLGCLPTLAKA
                     LGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPAPKTFLLSAKSC
                     VLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRACRTEREVRRQFG
                     RDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT          317 a          248 c          325 g          275 t
ORIGIN      
        1 gacagtgcgg aaaaccggca acatctggaa gaacatcagt tgttagacgg cattatgcgc
       61 aaagcctgcc gcaaccgtcc gctgacggaa gtgcaaacca aacgcaaccg gtatttgtcg
      121 aagacccgtt atagtggatt aaatttaaat caggacaagg cgacgaagcc gcagacagta
      181 caaatagtac ggcaaggcga ggcaacgccg tactggtttt tgttaatcca ctatatgtgg
      241 tcgaacagag cttcggtacg ctgcaccgta aattccgcta cgcccgggca gcctatttcg
      301 gactgattaa agtgagtgcg caaagccatc tgaaggcgat gtgtttgaac ctgttgaaag
      361 ccgccaacag gctaagtgcg cccgctgccg cctaaaaggc agcccggatg cctgattatc
      421 gggtatccgg ggaggattaa gggggtattt gggtaaaatt aggcggtatt tggggcgaaa
      481 atagacgaaa acctgtgttt gggtttcggc tgtcgggagg gaaaggaatt ttgcaaaggt
      541 ctcattatgt tatttccgag aattatcgca gcatcagcgc aacgtaagtt aagcgtttat
      601 ttgaaaaaag gcggtttggt cgcttatccg accgaatctt gttatggctt ggggtgtctt
      661 ccgacattag ccaaagcact cggtaagttg gcgcatttga aaaaaagacc gcaacacaag
      721 ggtatgattg tcatcgggaa tcaatttgaa caattgcagc ctttacttca aatgccgtct
      781 gaaaatcttc aggatatgct tagaaaagaa tggcctgccc ctaaaacgtt cctattatcc
      841 gccaaatcct gtgtcttacc tgagttgcgc gggaaacagc gcagcaagtt ggcggtcagg
      901 gttcccgccc atgtaggtgc gcgccgcttg tgtcaggctt tgcaaacgcc tttggtctcg
      961 acttcgtgca accgcgcagg caaacgcgcg tgtcggacgg agagggaagt cagaaggcaa
     1021 ttcggacgcg atgtgtggat tgttggtggg agaatcggca ggcagaaatc gccgagtcag
     1081 attattgacg gggaaacagg taaacgtttg cgttagatat gggtacttga atgaaaactt
     1141 ttttgcatac acaaagaaaa aattt
//
LOCUS       DAWFIE010000152         1152 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWFIE010000152 DAWFIE010000000
VERSION     DAWFIE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.153"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            243..1040
                     /gene="truA"
                     /locus_tag="WGQ71_002036"
     CDS             243..1040
                     /gene="truA"
                     /locus_tag="WGQ71_002036"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ0707970.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTAAVRPVQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGQMRQAAALLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          268 a          373 c          265 g          246 t
ORIGIN      
        1 tactggtttt tgttaatcca ctataaaatg gtttctttag attttacgtc ctagattccc
       61 gcctgcgcgg gaatgacgat tcgggcactc ctgacagggt aaattcacag gatagcgatt
      121 cgtagcaact gcatccaccc ccccaactcc ccaaacaacg ccgctcgccc tgggcgtttg
      181 ccgtttccct gcaaaatctg cgatacaatg cagtctgaac atttatccga atcccaaatc
      241 cgatggatac cgcacaaaaa caacgctggg caataaccct atcctatgat ggcagccgct
      301 tttacggctg gcagaaacag gctgacggcg taccgaccgt tcaggcggca ttggaaaccg
      361 cgctcgccca aatagcaggg gaagcggttt ccaccaccgt tgccggcagg accgataccg
      421 gcgttcatgc gactgcccaa gtcgttcatt ttgatactgc cgccgtccgt ccggttcagg
      481 catgggtgcg cggcgtaaat gcccacctgc ccgaaggcat tgccgttttg cacgcccgac
      541 aggtcgcccc cgaatttcac gcccgcttcg acgcatacgg acggcattac cgctacctgc
      601 tcgaatccgc ccccgtccgt tcccccctgc tcaaaaacag ggcaggctgg acgcatctca
      661 aactcgacat cgggcagatg cggcaggctg ccgccttatt gatcggcgaa caagacttct
      721 ccagcttccg cgccgccgaa tgccaagcaa aatcccccgt caaaaccatc taccgcgccg
      781 accttaccca aagctcagga ctcgtccgcc tcgatttgca cggcaacgcc tttttgcacc
      841 atatggtacg caacatcatg ggcgcgctcg tctatgtcgg cagcggcaga ctcagcgtcg
      901 aaggcttcgc cgcactgatt caagaacgca gccgcctcaa agccccgccg accttcatgc
      961 ccgacggact ttacctgacc ggcgtcgact atcccgaggc atacggcatc atccgccccc
     1021 aaatccccga atggctttaa aacattcttg tcgcggagat tttgaaatcg gacaaactgt
     1081 caggcaatct ttttcaatgt tgacactacc tcatcaaggt aataacattg ttattacata
     1141 aactggtgaa ta
//
LOCUS       DAWFIE010000153         1151 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWFIE010000153 DAWFIE010000000
VERSION     DAWFIE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.154"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..468
                     /locus_tag="WGQ71_002037"
     CDS             <1..468
                     /locus_tag="WGQ71_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0707971.1"
                     /translation="NTKNVQKNDNGNRQDLKTENQENGTFHAVSSLGLSAIYDFKLND
                     KFDKFKPYIGVRVAYGHVRHSIDSTKKTTNVVTVAGAANTAPTIYYAPETQNAYHESH
                     SIRRLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGVRYRF"
BASE COUNT          314 a          346 c          258 g          233 t
ORIGIN      
        1 aacacaaaaa atgtgcaaaa aaacgacaat ggcaacaggc aagacctgaa gacggaaaat
       61 caggaaaacg gtacattcca cgccgtttct tctctcggct tatccgccat ttacgatttc
      121 aaactcaacg ataaattcga taaattcaaa ccctatatcg gtgtgcgcgt cgcctacgga
      181 cacgtcagac acagcatcga ttcgaccaaa aaaacaacaa atgttgttac cgtcgccggt
      241 gctgctaaca cagcacctac gatttattat gcaccagaga cgcaaaacgc ctatcacgaa
      301 agccacagca tccgccgctt gggtcttggt gtcatcgccg gtgtcggttt cgacatcacg
      361 cccaagctga ccctggacac cggataccgt taccacaact ggggacgctt ggaaaacacc
      421 cgcttcaaaa cccacgaagt ctcattgggc gtgcgctacc gcttctgatt ccccgacacc
      481 gatgccgtct gaaccttcag acggcatttt tgatgcacct gccgttgaca ggcgcggggc
      541 gggcgtaggg aaatgcccga accgtcattc ccgacaacac cgcaatctcg aaattcgtca
      601 ttcccgcgca ggcgggaatc cagacctccg acgcggcggg aatctatcgg aaatgactga
      661 aaccccgaga ttctagattc ccacttccgt gggaatgacg gttcagttgc gttccgacaa
      721 caccgtaatc tcgaaacccg tccgacaaca ccgtaatctt gaaattcgtc attcccgcac
      781 aggcgggaat ccagacccct gacgcggcgg gaatctatcg gaaatgactg aaaccccgag
      841 attctagatt cccacttccg tgggaatgac gtggtgcagg tttccgtatg gatggattcg
      901 tcattcccgc gcaggcggga atccagacct gtccgcacag aaacttatcg gataaaaaca
      961 gttgcccaaa ccccgcgttc tagattccca ctttcgtggg aatgacggtt cagttgcgtt
     1021 ccgacaacac cgtaatctca aaacccgtcc gacaacaccc caatcttgaa attcgtcatt
     1081 cccgcacagg cgggaatcca gacctgtccg cacagaaact tatcggataa aaacagttgc
     1141 tcaaaccccg c
//
LOCUS       DAWFIE010000154         1075 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWFIE010000154 DAWFIE010000000
VERSION     DAWFIE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.155"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(124..552)
                     /locus_tag="WGQ71_002038"
     CDS             complement(124..552)
                     /locus_tag="WGQ71_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ0707972.1"
                     /translation="MAFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(533..967)
                     /locus_tag="WGQ71_002039"
     CDS             complement(533..967)
                     /locus_tag="WGQ71_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ0707973.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          282 a          322 c          194 g          277 t
ORIGIN      
        1 atttgaaaac cccattaaac cgtctttaac ccatcgtcct gaacggtaga tgtttcaccc
       61 cctgcgaacc aaaaaaaaga ccgtctgaat acagacggcc aaagcctggt cacacatcac
      121 tgcctaaaat aaagctgcct gctgtgccgc aggtcggctc atttcattga taatcgtata
      181 tcccgttcgt gaagagatgc cgtatttagg gcatagcttc gtcatcgcca taagcccgct
      241 ctccttatca atatcgcgca atttgacaaa ctcctgataa aacctatggt ttctcaactg
      301 tattaacgcc ttaccgcacc gtgggacata caattcctcg ccaccatata cctgcaacag
      361 ctcatgtgtt ttcacttcgc cgatggcttc gaccaaaatt gccaaacgct cggtgtccac
      421 cttgccctta ccaaatttaa accgcgcccc gccaatcgcc ttgaccagct gttccgtcgc
      481 tgccagtccg atgacatcca caatgtccaa cacggtatcc ggcaataaat gttcaacttt
      541 ttcgaacgcc atcatcccca cccgcttttt ccgttttcct gttttccgca atctgcaacg
      601 cagcaaccag tttatgtagc tgcgtatcgt ctaaatattc gaccttatcc ttaccaaaca
      661 tccgccgcgc cattgcgtgt gcatagttcc aatgtttgcc gccgacggtc agcagggctt
      721 cgactttgtc caacattgcc gctgatgatg tccgacgcag atgcggtttg ccgtgtgggt
      781 taccttttgc tttaggctta aatccgtgcg accgcatatc agcgacaaca gactcaagtt
      841 cggaaacatc catatccgca cacgaccgct tgcccgtcac acgctccaac accgcgcgat
      901 aggtaccgtc gtccaagccc agctcctttt gagcaatctt aattttcgca atcaacgccc
      961 ggcgcatctc aaacccataa aacacaatat attgactaat taacgcatat tataccaata
     1021 aaatacaata tatagtatta agccgatgtt ttttgcgaaa cggacagaca taaaa
//
LOCUS       DAWFIE010000155         1045 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWFIE010000155 DAWFIE010000000
VERSION     DAWFIE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.156"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            360..>1045
                     /locus_tag="WGQ71_002040"
     CDS             360..>1045
                     /locus_tag="WGQ71_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0707974.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRIR
                     YLRDHRGRPAYPLLSMFKTVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLKLINRQLTEKGLKIEKASAAVVDATIIQTAGSKQRQAIE
                     VDEEGQINGQTTPSKDKDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECK
                     HLSPLLEGIA"
BASE COUNT          300 a          293 c          206 g          246 t
ORIGIN      
        1 tgggtcagtt gcgtgtagct catggcaatc tttcttgcag gaaaggccgt atgctaccgc
       61 atactggcct ttttctgtta gggaaagttg cacttcaaat gcgaatccgc ctcctgatgt
      121 aatagtcgta gaagataatt gccctttatt cggaatattc ctaaaatcag gcctctgccc
      181 tgaatattgt tcatacaaat caaatatttc tcttcgactt acgttagaat atatcggcat
      241 tccatttttg ctggcagatg aatttggtct gatcgtttta aaaccaattt gtaagttgag
      301 acctttgcaa aatccccaaa atcccctaaa ttcccatcaa gacatttagg ggattttcca
      361 tgagcacctt ctttcagcaa accgcacaag ccatgattgc caaacacatc gaccgcttcc
      421 cactattgaa gctagatcaa gtgattgatt ggcaaccgat cgaacaatac ctgaaccgtc
      481 aaagaatccg ttacctccga gaccaccgcg gccgtcccgc ctatccgctg ctatccatgt
      541 tcaaaaccgt cctgctcgga caatggcaca gcctctccga tcccgaactc gaacacagcc
      601 tcatcacccg catcgatttc aacctgtttt gccgttttga cgaactgagc atccccgatt
      661 acagcacctt atgccgctac cgcaactggc tggcgcaaga cgataccctg tccgaattgc
      721 tcaaactgat caaccgccaa ctgaccgaaa aaggtttaaa aatagagaaa gcatccgccg
      781 ccgtcgttga cgccaccatt attcagaccg ccggcagcaa acagcgtcag gccatagaag
      841 tcgatgaaga aggacaaatc aacggccaaa ccacaccgag taaagacaaa gatgcccgtt
      901 ggatcaagaa aaacggcctc tacaaactcg gttacaaaca acatacccgt accgatgcgg
      961 aaggctatat cgagaaactg cacattactc ccgccaatgc ccatgagtgc aaacacctgt
     1021 cgcctttgct ggaagggata gccga
//
LOCUS       DAWFIE010000156         1019 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWFIE010000156 DAWFIE010000000
VERSION     DAWFIE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.157"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT          310 a          208 c          223 g          278 t
ORIGIN      
        1 ccgccttgtc ctgatttttg ttaatccgag acctttgcaa aaatagtctg ttaacgaaat
       61 ttgacgcata aaaatgcgcc aaaaaatttt caattgccta aaaccttcct aatattgagc
      121 aaaaagtagg aaaaatcaga aaagctttgc attttgaaaa tgagattgag cataaaattt
      181 tagtaaccta tgttattgca aaggtctcaa tccactataa agaccgtcgg gcatctgcag
      241 ccgtcattcc cgcgcaggcg ggaatctagt ccgttcggtt tcggtttttt ggctagtgcc
      301 gcaacattaa atttctagat tcccactttc gtgggaatga cgcgattaga gtttcaaaat
      361 ttattctaaa tagctgaaac tcaacgcatt ggattcccgc ctgcgcggga atgacgaatt
      421 tcaggttgct gtttttggtt ttctgttttt gtgaaaataa cgggatttca gcttgtgggt
      481 atttactgga aaaaacagaa accgctccgc cgtcattccc gcgcaggcgg gaatccagac
      541 attcaatgct aaggcaattt atcgggaatg actgaaactc aaaaagctgg attcccactt
      601 tcgtgggaat gacgaagtgg aagttacccg aaacttaaaa caagcgaaac cgaacgaacc
      661 ggattcccac tttcgtggga atgacgggat gcaggtttcc gtatggatgg attcgtcatt
      721 cccgcgcagg cgggaatcta gacattcaat gctaaggcaa tttatcggaa atgactgaaa
      781 ctcaaaaaac tggattccta ctttcgtggg aatgacgcga ttagagtttc aaaatttatt
      841 ctaaatagct gaaactcaac gcactggatt cccgcctgcg cgggaatgac gaagtggaag
      901 ttacccgaaa cttaaaacaa gcgaaaccga acgagccgga ttcccgcttg cgcgggaatg
      961 acgggattaa gttttcaaaa ttcatcagaa attactgatt taatagcata aatttttta
//
LOCUS       DAWFIE010000157          857 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWFIE010000157 DAWFIE010000000
VERSION     DAWFIE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.158"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(10..576)
                     /locus_tag="WGQ71_002041"
     CDS             complement(10..576)
                     /locus_tag="WGQ71_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ0707975.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGS
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMREGLSGKAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTAGRALGVLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          236 a          277 c          183 g          161 t
ORIGIN      
        1 tttccgacat cattgaatca aacccaaatg cgacaagagc gtccatgtac cgatggcaat
       61 caacaccaaa cctccggcaa attccgcacg cctgccgaac aatacgccca aagcccttcc
      121 cgccgtcagc ccgaccgcca ccatcaccgt cgtcgccata ccgatgattg cggcggcaaa
      181 ggcgatgttt acctccataa acgccaagcc caccccgact atcatggaat caatactggt
      241 tccaaaagca gtcaaaaccg tcatccatag gctttcccgt ttgctttcgc gcacatcttc
      301 cgccttgccg gacagccctt cgcgcatcat tttcagaccc agcccgccca gcaggacgaa
      361 agccgcccaa tggtcccatt cgctgataaa cggtttggca taaaaaccgc ctacccagcc
      421 tgccagcggc gtgagtgctt caaccgagcc gaacaccaag gctgttgctg caattttgcg
      481 cggaggcatc ctgaccgccg caccctttgc caatgcgacg gcaaacgcat ccatcgacat
      541 ccccagcgca atcaagagca aagcgtaaag actcataccg cacccatcct caaaaagggc
      601 agattatagc aaaacagcaa agcgcgaaaa acgccgcacg aaaacccaca tcccgtcatt
      661 cctgcaaaaa caaaaaatca aaaacagaaa tcccgtcatt cccgcgcagg cgggaatcca
      721 gaccggtcgg tgcagaaact tattggataa aacggtttct ttagatttta cgttctagat
      781 tcccactttc gtgggaatga cgggatgtag gtttccgtgc ggatggattc gttattcccg
      841 cgcaggcggg aatccag
//
LOCUS       DAWFIE010000158          821 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWFIE010000158 DAWFIE010000000
VERSION     DAWFIE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.159"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            153..542
                     /locus_tag="WGQ71_002042"
                     /pseudo
     CDS             153..542
                     /locus_tag="WGQ71_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          203 a          182 c          227 g          209 t
ORIGIN      
        1 aaatttttat agtggattaa caaaaaccag tacagcgttg cctcgcctta gctcaaagag
       61 aacgattctc taaggtgctg aagcaccaag tgaatcggtt ccgtactgtt tgtactgtct
      121 gcggcttcgt cgccttgtcc tgatttttgt taatccacta tacctccgcc aatgcccatg
      181 agtgcaaaca cctgtcgccg ttgttagaag ggatagctga aggtacgacc gtctatgccg
      241 acaaaggcta tgacagtgcg gaaaaccggc aacatctgga aaagcgtcag ttgttagacg
      301 gtattatgcg caaagcctgc cgcaaccgtc cgctgacgga ggcacagacc aaacgcaacc
      361 ggtatttgtc gaagacccgt tatgtggtcg aacaaagctt cggtacgctg caccgtaaat
      421 tccgctatgc ccgggcagcc tatttcggac tgattaaagt gagtgcgcaa agccatctga
      481 aggcgatgtg tttgaacctg ttgaaagccg ccaacaggct aagtgcgcct gctgccgcct
      541 aaaaggcggc tcggatgcct gattatcggg tatccgggga ggattcaggg ggtatttggg
      601 tagaattagg gagtgattgg tggcgaaaac agctgaaaac ctgtgtttgg gtttcggctg
      661 tcgggaggga aaggaatttt gcaaaggtct cagattatct gaaagtccga gattctagat
      721 tcccgctttc gcgggaatga cggttcagtt gctacggtta ttgtcaggtt tcggttatgt
      781 tggaatttcg ggaaacttat gaattgtcat tcccacgcag g
//
LOCUS       DAWFIE010000159          740 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWFIE010000159 DAWFIE010000000
VERSION     DAWFIE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.160"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..234
                     /locus_tag="WGQ71_002043"
     CDS             <1..234
                     /locus_tag="WGQ71_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0707976.1"
                     /translation="LIAQKEFEEFVNNVKEYDILESFSYFLNKRVAHNIWKIYFSDES
                     VIFIRKSEENGKTVHEFVYQEYTDSSDFNSMFE"
     gene            176..616
                     /locus_tag="WGQ71_002044"
     CDS             176..616
                     /locus_tag="WGQ71_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707977.1"
                     /translation="MNSFIKSTPIVLILILCSSNSYSEPVRLERSQIKFQSSNNLKSS
                     GFKLDSSSKSFAKFIEAANFEHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKF
                     STRAVPYVGTALLAHDVYETFKEDIQARGYQYDTETDKFAKVSG"
BASE COUNT          240 a          135 c          161 g          204 t
ORIGIN      
        1 ttaattgctc agaaagaatt tgaagaattt gttaataacg taaaagagta cgatatttta
       61 gagagtttct cttatttctt aaataagaga gtcgcacata atatttggaa gatttatttt
      121 tctgatgaat ctgttatttt tataagaaaa tcagaagaga acggaaaaac tgttcatgaa
      181 ttcgtttatc aagagtacac cgatagttct gattttaatt ctatgttcga gtaattctta
      241 ttctgaacct gtaagattag aaagatcaca aattaaattt caatcaagta ataatttaaa
      301 atcaagtggt tttaaattag attcaagttc taagagtttt gccaaattca tagaagcggc
      361 aaattttgag cacatcccta cgggcgcaaa agcccgaatc aacgccaaaa taaccgccag
      421 cgtttcccgc gccggcgtat tggcgggggt cggcaaactt gcccgcttag gcgcgaaatt
      481 cagcacaagg gcggttccct atgtcggaac agccctttta gcccacgacg tatacgaaac
      541 tttcaaagaa gacatacagg cacgaggcta ccaatacgac accgaaaccg acaaatttgc
      601 aaaggtctca ggctaagtgc gcctgttgcc gcctaaaagg taacccggat gcctgattat
      661 cgggtatccg gggaggatta agggggtatt tgggtaaaat taggaggtat ttggggtgaa
      721 aacagccgaa aacctgtgtt
//
LOCUS       DAWFIE010000160          693 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWFIE010000160 DAWFIE010000000
VERSION     DAWFIE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.161"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..563)
                     /locus_tag="WGQ71_002045"
     CDS             complement(<1..563)
                     /locus_tag="WGQ71_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ0707978.1"
                     /translation="MTKLYAEIAKREAQDDGTVKVLGYASSEAVDSDGEVIATAAMKA
                     AIPDYMKFDAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDNLNKSQITGLKLTEISLVERPANPDAVFTCFKADKGAEAVNNDTEHNAT
                     YFSHFPSKQKSTGGSRCPFLYRFPYFFT"
BASE COUNT          154 a          182 c          171 g          186 t
ORIGIN      
        1 gtgaaaaaat aggggaacct gtaaaggaag gggcatcggc tgccgccggt gcttttttgt
       61 ttggaaggga aatggctaaa atatgtagca ttatgttctg tatcgttgtt taccgcttcc
      121 gcacctttgt ccgccttaaa gcaggtaaac accgcatcgg gattggcagg gcgctcgaca
      181 aggctgattt ccgtcagctt caagcccgtg atttgcgact tgttcaaatt atcgcgggcg
      241 gtaacgctgc cgccgatgga aaagcctttg taaacgcctg ttttgacttt cgacacgaca
      301 acggggtcga tgatatgagc accgaaaaag gttatgccgt cgtcttcaac gttgatttca
      361 attgccgttc ccgcagcgtt tgagccgtgc atctcgcgcc ccgcgtcaaa cttcatataa
      421 tcgggaatcg ccgccttcat agctgccgtc gcgatgactt caccgtccga atcgaccgcc
      481 tcgcttgagg cataacccaa aaccttgacc gtgccgtcgt cctgcgcctc cctcttggcg
      541 atttctgcgt ataactttgt catgggttta ctcctgcctg tataaatggc atgctgaatt
      601 taaagccctt ttgtaaggct ggaagtgtac ttgccttaac gctatccgct ccagaacgac
      661 actgcaaaag ggctttactc atccttcaag atg
//
LOCUS       DAWFIE010000161          613 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWFIE010000161 DAWFIE010000000
VERSION     DAWFIE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.162"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            323..>613
                     /locus_tag="WGQ71_002046"
     CDS             323..>613
                     /locus_tag="WGQ71_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0707979.1"
                     /translation="MQVFLTIPGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYI
                     DEVLTAGHGKLYQSDGRHSVNPTEGYGTGGLLQGKKHMLSLTWNAPIEAFTRE"
BASE COUNT          177 a          152 c          155 g          129 t
ORIGIN      
        1 tgcgcgggaa tgacaactca ttagttacct aaaacttaaa aagcagaaac ctttacgccg
       61 tcattcccac gaaagtggga atccgggaac ttaacgttac agtgatttat cggaaacggc
      121 tgaaaccgaa tgaattggat tcccgcctgc gcgggaatga cgaatccatc cgtacggaaa
      181 cctgcaccac gtcattccca cgaacctaca ttccgtcatt cccacgaaag tgggaatcca
      241 ggacgaaaaa tctccagaaa ccgttttacc cgataagttt ccgcaccgac aggtctggat
      301 tcccgcctgc gcgggaatga cgatgcaggt atttctgacg attcccggct atgatgttga
      361 ggcagaaatc gaaaaattcg tttggatgga tgctgtgatt tggcagatgc cgggctggtg
      421 gatgcacgag ccttggacag tgaaaaaata catagacgaa gtattaaccg ctggacacgg
      481 caaactctac caaagcgacg gcagacacag cgtcaatccg actgagggct acggcacagg
      541 cggcttgttg caaggcaaaa aacatatgct ttcactgact tggaatgcgc cgattgaggc
      601 gtttacccgc gaa
//
LOCUS       DAWFIE010000162          611 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWFIE010000162 DAWFIE010000000
VERSION     DAWFIE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.163"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT          162 a          149 c          128 g          172 t
ORIGIN      
        1 gacggcggcg ggatttctgt ttttccgata aattcctaaa acttaaaatt ccgttattcc
       61 tacggaaaca gaaaccaaaa acagaaacct aaaatctcgt cattcccacg caggcgggaa
      121 tccagtccgt tcggtttcgc ttgttttaag tttcgggtaa cttccacttc gtcattccca
      181 cgaaagtggg aatccagtcc gttgagtttc agtcatttcc gataaatcac cgcaacgtta
      241 aatttctaga ttcccgcctg cgcgggaatg acggcggggg gggtttgttg tttcggataa
      301 atccctgtag tttttcgttt ctagattccc gctttcgcgg gaatgacggc ggggggattt
      361 ctgtttttcc gacaaattcc taaaacttaa aattccgtta ttcccgcgag aacagaaaac
      421 caaaaacaga aacctaaaat ctcgtcattc ccgcaaaaac agaaaaccaa aaaacagcaa
      481 cctaaaattc gtcattcccg cgcaggcggg aatctagtcc gttcggtttc gcttgtttta
      541 agtttcgggt aacttccact tcgtcattcc cgcgaaagtg ggaatccagt ccgttgagtt
      601 tcagtcattt c
//
LOCUS       DAWFIE010000163          575 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWFIE010000163 DAWFIE010000000
VERSION     DAWFIE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.164"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            190..>575
                     /locus_tag="WGQ71_002047"
     CDS             190..>575
                     /locus_tag="WGQ71_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HEZ0707980.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQQYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYDSGLTKTST"
BASE COUNT          164 a          129 c          162 g          120 t
ORIGIN      
        1 gctgatgccg gagacgtata ttgcgtctat aacatcagac tgaagcagta cactgcctgc
       61 caggttaccc gagttgaaga acacggtggc aaaaaaacac atgcgaccct gctggctttg
      121 gactggcagg gcaacaaacc gcttggggcg gcggaactgg cggatttgaa accgctctac
      181 aaagacttta tgtattggga acgcggtctg cacatgtata aggcaagtgc cgtcgtgccg
      241 acgggatatg tacgggttgg gaataccgcg ccgctggtcg gcgaagacac gcagcagtat
      301 gcctcttttt ggggcgacgg ctacgacgtg taccgtcagt tgagatggca gcagataccc
      361 gaaaaacaga gaaaggcatt caaaaaagcc gccaaaagca aaaagaccgt gatgtttgcc
      421 ggacgggaat acggcatatc caaacagaat ttgagcgatg tttgggacga ttttgaagac
      481 gcgatggaac tgaaggcgtt tccctgcctg tcttcgctgt ttctgaccaa atggcataaa
      541 aatctatatg atagtggatt aacaaaaacc agtac
//
LOCUS       DAWFIE010000164          567 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWFIE010000164 DAWFIE010000000
VERSION     DAWFIE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.165"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            158..567
                     /locus_tag="WGQ71_002048"
                     /pseudo
     CDS             158..567
                     /locus_tag="WGQ71_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          171 a          151 c          127 g          118 t
ORIGIN      
        1 cggaatatag cggattaaca aaaaccagta cggcgttgcc tcgacttagc tcaaagagcg
       61 attctctaag gtgctgaagc accaagtgaa tcggttccgt actatttgta ctgtctgcgg
      121 cttcgtcgcc ttgtcctgat ttttgttaat ccaatatact ttatgccgct accgcaaccg
      181 actggctcaa gacaataccc tgtctgaact gttggaactg attaaccgcc aactgaccga
      241 aaaaggttta aaaatagaga aagcatccgc cgccgtcgtt gacgccacca ttattcagac
      301 cgctggcagc aaacagcgtc aggccataga agttgacgaa gaaggacaaa tcagcggcca
      361 aaccacaccg agtaaagaca gcgatgcccg ttggataaag aaaaacggcc tctacaaact
      421 cggttacaaa caacataccc gtaccgatgt ggaaggctat atcgagaaac tgcacattac
      481 ccccgccaat gcccatgagt gcaaacacct gtcgccgttg ttggaaggac tgcccaaagg
      541 tacgaccgtc tatgccgaca aaggcta
//
LOCUS       DAWFIE010000165          563 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWFIE010000165 DAWFIE010000000
VERSION     DAWFIE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.166"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(25..348)
                     /locus_tag="WGQ71_002049"
     CDS             complement(25..348)
                     /locus_tag="WGQ71_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ0707981.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(336..563)
                     /locus_tag="WGQ71_002050"
     CDS             complement(336..563)
                     /locus_tag="WGQ71_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ0707982.1"
                     /translation="MRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCPTVRR
                     GWAEAAAMLVETEQEHFFSEIENEADKEWIW"
BASE COUNT          144 a          147 c          118 g          154 t
ORIGIN      
        1 acacaatcca ttcagactat tcaatcaggc aaacatctcc tgcaatactg caaacagttt
       61 ttcagccgta ctgttgtcta aattgccaag atgcttgacc aatccggctt tatccacagc
      121 cctaatctgt tcgggcaaaa gcaaaccgtc tttatcctga aagcggacat tgacgcggaa
      181 cggggcagga cggcttccgc tcgtcatggg aacgatcagc acagtcttga gatagttgtg
      241 tatttcagga ggagagacta cgacacaagg acgtgtcttt ttgatttcgc ttcctacggt
      301 cgggtctaag gagaccagat agattccgcc gcgtactacc atatccattc tttatccgct
      361 tcgttttcaa tttcggaaaa aaaatgctcc tgctcggttt cgacaagcat tgcggcagct
      421 tctgcccatc ccctgcgaac ggtaggacag cttaaaataa tattgccctt ttcaactgta
      481 acagccaagc tgtctactgc ccctatttga cccaataatg atttgggcag aatcacgcct
      541 tgcgagtttc ccattttgcg tat
//
LOCUS       DAWFIE010000166          561 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWFIE010000166 DAWFIE010000000
VERSION     DAWFIE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.167"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT          127 a          125 c          156 g          153 t
ORIGIN      
        1 atttagggct aatctagtac agccccaaaa tttaaatcag gacaaagcga cgaagccgca
       61 gacagtacaa atagtacgga accgattcac ttggtgcttc agcaccttag agaatcgttc
      121 tctttgagct agggcgaacc aacgctgtac tggtttagat ttaattcact atacctgcgt
      181 aatggtatcg ggtactcatc atcaacctgc cttgcattcg gttgtgttgt ccggcaactg
      241 tccggttgtg tcgatgggtt gccctgccgc tccctgtgct gcgcaacggt tgtgtgcctt
      301 gggtgggcgc gggtggcggc cgggcggttt gttgctatgg tacgggagtt ccgatataca
      361 agggtgggct tcagcccacc aatcaacgac cgaaaaagaa aaaacgttgg gctgaagccc
      421 actctacgca attacgcaac tgttatcgtg gggatgacgg gattttaggt ttttgttttt
      481 ctgtttttgc gggaaggatg ggttttaggt tgcggtcatt tatcggaaaa gcagaaatcg
      541 ctccgccgtt attccagcga a
//
LOCUS       DAWFIE010000167          524 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWFIE010000167 DAWFIE010000000
VERSION     DAWFIE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.168"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT          130 a           98 c          163 g          133 t
ORIGIN      
        1 atgtaggttc gtaggaatga cgggatgtag gtttccgtat ggatggattc gtcattcccg
       61 cgcaggcggg aatctaggtc tgtcggtgcg gaaacttatc gggtaaaacg gtttcttgag
      121 attttgcgtc ctggattccc actttcgtgg gaatgacggg atgtaggttc gtaggaatga
      181 cgcggtgcag gtttccgtac ggatggattc gtcattcccg cgcaggcggg aatctagaca
      241 ttcaatgcta aggcaattta tcggaaatga ctgaaactca aaaaactgga ttcccacttt
      301 cgtgggaatg acggaatgca ggttcgtggg aatgacggaa tgcaggttcg tgggaatgac
      361 ggaatgcagg ttcgtgggaa tgacggaatg caggttcgtg ggaatgacgc ggtgcaggtt
      421 tccgtatgga tggattcgtc attcccgcgc aggcgggaat ctagacattc aatgctaagg
      481 caatttatcg gaaatgactg aaactcaaaa aactggattc ccac
//
LOCUS       DAWFIE010000168          520 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWFIE010000168 DAWFIE010000000
VERSION     DAWFIE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.169"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..360)
                     /locus_tag="WGQ71_002051"
     CDS             complement(<1..360)
                     /locus_tag="WGQ71_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEZ0707983.1"
                     /translation="MRKILVTGGAGFIGSAVVRHIIQNTQDSVVNLDKLTYAGNLESL
                     TDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNI
                     VGTFNLLEAARAYWQQMP"
BASE COUNT          130 a          119 c          144 g          127 t
ORIGIN      
        1 cggcatttgt tgccagtagg cgcgggctgc ttcaagcaga ttgaatgtgc cgacgatatt
       61 ggtttggata aactcgcctg ccgaaccgat agagcggtcg acatggcttt ccgccgccaa
      121 gtgcatcacg gcatctggcc ggtgttgtgc gaatacgcgg tcgagttcgg cgcggtcgca
      181 aatgtccact tgctcgaaag catagcgggg attatcggca atgtcggtca gcgattcgag
      241 attgcccgcg taggtcagtt tgtcgaggtt gacgacggaa tcttgggtgt tttgaatgat
      301 atggcggaca acggcagagc cgataaagcc cgcaccgccg gtaacaagga tttttctcat
      361 aaatttcaga ggatagccaa aaaatataaa cagattatag cagacagaat gtgtgttttt
      421 cagataaaga ggccgtctga aaacatctct ttcagacggc ctgtatcagg tcaacttaat
      481 cgtcgtagcc attcggatta ttactcaccc agcgccatga
//
LOCUS       DAWFIE010000169          520 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWFIE010000169 DAWFIE010000000
VERSION     DAWFIE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.170"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT          138 a           95 c          143 g          144 t
ORIGIN      
        1 cttttgcggg aatgacggtt cagttgctac ggttactgtc aggtttcggt tatgttggaa
       61 tttcgggaaa cttatgaatc gtcattcccg cgtaggcggg aatctagacc ttagaacaac
      121 agcaatattc aaagattatc tgaaagtccg agattctaga ttcccacttt cgtgggaatg
      181 acgggatgta ggttcgtggg aatgacgtgg tgcaggtttc cgtgcggatg aattcatcat
      241 tcccgcgcag gcgggaatct agactttaga acaacagcaa tattcaaaga ttatctgaaa
      301 gtctgagatt ctagattccc actttcgtgg gaatgacggg atgtaggttc gtgggaatga
      361 cgtggtgcag gtttccgtgc ggatggattc gtcattcccg cgcaggcggg aatctagact
      421 ttagaacaac agcaatattc aaagattatc tgaaagtctg agattctaga ttcccacttt
      481 cgtgggaatg acgaaaagtt gcgggaatga cgaaaagttg
//
LOCUS       DAWFIE010000170          507 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWFIE010000170 DAWFIE010000000
VERSION     DAWFIE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.171"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT          154 a          130 c          109 g          114 t
ORIGIN      
        1 gctaacaagg caaattatac cataaatttt aaataaatca agcggtaaat gattttccac
       61 ggccactcgg attaatccgg gtggcttcat ttttaaaggt ttgcaaggca agcggggtcg
      121 tccgttttgg tggaaataat atatcgatcg cgcttcaagg ctctgcgcgt acctcattgc
      181 cgcccgttta aacacggggt ttatctgaca ggcgcacaat ccaccctctc atttaccgaa
      241 caagcggact agattccctc ctactcggga atgatgaagc tatccgcacg gaaacctgca
      301 ccacgtcatt cccacaagaa cagaaaacca aaaacagaaa cctaaaattc ggcattcccg
      361 cgaaagtggg aatccagaac gcaaaatctc aagaaaccgt tttcccgata agtttctgca
      421 ccgacagacc tggattcccg cctgcgcggg aatgacggca accggccggt tgcgtatcaa
      481 aaaataaagt aattcggcta gatatag
//
LOCUS       DAWFIE010000171          498 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWFIE010000171 DAWFIE010000000
VERSION     DAWFIE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.172"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            22..>498
                     /locus_tag="WGQ71_002052"
     CDS             22..>498
                     /locus_tag="WGQ71_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Opacity protein opA54"
                     /protein_id="HEZ0707984.1"
                     /translation="MQADLAYAAERITHNYPEPTGADKDKISTVSDYFRNIRAHSIHP
                     RVSVGYDFGGWRIAADYASYRKWKESNSSTNTENSETQQNRIKIETGHQGNGSFHAAS
                     SLGLSAIYDFKLNDKFKPYIGARVAYGYVKHQVHSVESKTKTVTPKPNGGPVKEGPT"
BASE COUNT          155 a          134 c          111 g           98 t
ORIGIN      
        1 gacaatggcc gcggcccgta tgtgcaggca gatttagcct acgccgccga acgcattacc
       61 cacaattatc cggaaccaac cggtgcagac aaagacaaaa taagcacagt aagcgattat
      121 ttcagaaaca tccgtgcgca ttccatccac ccccgggtgt cggtcggcta cgatttcggc
      181 ggctggagga tagcggcaga ttatgccagt tacagaaaat ggaaagaaag taattcttct
      241 actaatacag aaaatagcga gactcaacag aaccgcataa agattgaaac aggacatcag
      301 ggaaacggca gcttccacgc cgcttcttct ctcggcttat ccgccattta cgatttcaaa
      361 ctcaacgata aattcaaacc ctatatcggc gcgcgcgtcg cctacggata cgttaagcat
      421 caggttcatt cagtggaaag caaaaccaag actgttaccc ctaaaccaaa tggaggccct
      481 gtcaaagaag gtccgacc
//
LOCUS       DAWFIE010000172          488 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWFIE010000172 DAWFIE010000000
VERSION     DAWFIE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.173"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(121..>488)
                     /locus_tag="WGQ71_002053"
     CDS             complement(121..>488)
                     /locus_tag="WGQ71_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707985.1"
                     /translation="GFGQDTVYNYHVDKNSDTMHFKGFKAADVHFIRSGSDLVLSASE
                     QDNVRISGFFYGENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGG
                     NVDANIQSVQQPLLVTPSA"
BASE COUNT          160 a          101 c           88 g          139 t
ORIGIN      
        1 agaagtgcac cgattaaggt taattaaaat acgaccctta tcccaatatt aagaaaaaca
       61 gcaatcaaaa taaacttgat tgctgttttt cagtttaagc catgaatgtg attaggctcc
      121 ttatgcagat ggcgttacca ataacggctg ctgtacggat tgtatattgg catccacatt
      181 tcctcctgtc gcagcagtat tagaaccgaa cacagacata gactgtacca aattattgcc
      241 agcattaata tacttggcaa aatctggatt actgatagct gcatcatcaa agacaaatgt
      301 atctacacga tggttttcac catagaagaa tccggaaata cgtacgttgt cttgttcaga
      361 agcgctaagc accaaatcac ttccggaacg gataaaatga acatctgctg ctttaaatcc
      421 tttaaagtgc atagtgtcag agtttttatc cacatggtaa ttatagaccg tatcctgacc
      481 gaagcctt
//
LOCUS       DAWFIE010000173          485 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWFIE010000173 DAWFIE010000000
VERSION     DAWFIE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.174"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(139..321)
                     /locus_tag="WGQ71_002054"
     CDS             complement(139..321)
                     /locus_tag="WGQ71_002054"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707986.1"
                     /translation="MPSEPSDGIFDSPAVDKRGAGVGKCPNRHSRQHRNLETRHSRQY
                     RNLEIRHSRAGGNPDP"
     gene            complement(335..>485)
                     /locus_tag="WGQ71_002055"
     CDS             complement(335..>485)
                     /locus_tag="WGQ71_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0707987.1"
                     /translation="LGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLG
                     MRYHF"
BASE COUNT          106 a          112 c          149 g          118 t
ORIGIN      
        1 tttcaagatt gcgctgttat cgggaatgac ggatttcaag attacggtgt tgtcggaatg
       61 caactgaacc gccattccca cgaaagtggg aatctagaat ctcggggttt cagtcatttc
      121 cgatagattc ccgccgcgtc aggggtctgg attcccgcct gcgcgggaat gacgaatttc
      181 gagattgcgg tattgtcggg aatgacgggt ttcaagatta cggtgttgtc gggaatgacg
      241 gttcgggcat ttccctacgc ccgccccgcg tttgtcaacg gcaggtgaat caaaaatgcc
      301 gtctgaaggt tcagacggca tcggtgtcgg ggaatcagaa gtggtagcgc atgcccaatg
      361 agacttcgtg ggttttgaag cgggtgtttt ccaagcgtcc ccagttgtgg taacggtatc
      421 cggtgtccaa ggtcagcttg ggcgtgatgt cgaaaccgac accggcgatg acaccaagac
      481 ccaag
//
LOCUS       DAWFIE010000174          464 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWFIE010000174 DAWFIE010000000
VERSION     DAWFIE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.175"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            116..>464
                     /locus_tag="WGQ71_002056"
     CDS             116..>464
                     /locus_tag="WGQ71_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0707988.1"
                     /translation="MFGFRLSGGKGILQRSQVALVVTESTGGLEIPAAKAIRRAGIAV
                     IIANPRQTHQFAQSQPLTKTDAKDAKMPAFFTQMMTQKEDWQTMPYQPPTEAEEVLEA
                     LVNRRNQSADMRTA"
BASE COUNT          119 a          126 c          133 g           86 t
ORIGIN      
        1 gcctgttgcc gcctaaaagg cgacccggat gcctgattat cgggtatccg gggaggatta
       61 aagggatatt tgggtagaat taggcggtat ttggggcgaa aacagctgaa aacctgtgtt
      121 tgggtttcgg ctgtcgggag ggaaaggaat tttgcaaagg tctcaggtcg ccctcgtcgt
      181 gacggaaagt actggcggtc tcgaaatccc cgccgccaaa gccatccgcc gagcaggcat
      241 agccgtgatt atcgccaacc cgcgtcagac gcatcagttt gcccaatcgc agccgctgac
      301 caaaaccgac gccaaagatg ccaaaatgcc cgccttcttc acacagatga tgacgcagaa
      361 agaagattgg caaaccatgc cctaccaacc gcccaccgaa gcggaagaag tgttggaagc
      421 cttggttaac cgccgcaacc aatcggcgga tatgcggact gccg
//
LOCUS       DAWFIE010000175          460 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWFIE010000175 DAWFIE010000000
VERSION     DAWFIE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.176"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT          119 a          130 c           99 g          112 t
ORIGIN      
        1 ttgaaaagct tacctcccca ttttaacttt gcacactgat cacggtaaag cgttttttag
       61 caaaccgctg cagatgccca acggtctgga ttcccgcctg cgcgggaatg acggtttaga
      121 agttgcccga aacctcaaaa aaaaccgaaa ccgaacaagc cggattcccg cctgcgcggg
      181 aatgacgaat ccatccgcac ggaaacctgc accgcgtcat tcccgcgaac ctacattccg
      241 tcattcccgc gaacctacat cccgtcattc ccacgaaagt gggaatctag aatctcggac
      301 tttcagataa tctttgaata ttgccgctgt ccaatggtct agattcccgc ctacgcggga
      361 atgacgggtc ttttataatc tttgaatatt gctgttgttc taaggtctag attcccgcct
      421 acgcgggaat gacggtttag aagttgcccg aaacctcaaa
//
LOCUS       DAWFIE010000176          444 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWFIE010000176 DAWFIE010000000
VERSION     DAWFIE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.177"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT          124 a          109 c           90 g          121 t
ORIGIN      
        1 tttgcgggaa tgacggcggc gggatttctg tttttccgac aaattcctaa aacttaaaat
       61 ttcgttattt ccgcgagaac agaaaaccaa aaacagaaac ctaaaatctc gtcattccca
      121 cgcaggcggg aatccagtct gttgagtttc agtcatttcc gataaatcac cgcaacatta
      181 aatttccaga ttcccgcttt cgcgggaatg acggcggggg ggatttctgt ttttccgaca
      241 aattcctaaa acttaaaatt tcatcatttc tgcgaagaca gaaaaccaaa aacagaaacc
      301 tgaaaacagc aacctaaaat tcgtcattcc cgcgcaggcg ggaatccagt ccgttcggtt
      361 tcgcttgttt taagtttcgg gcaacttcca ctttgtcatt cccgcgcagg cgggaatcca
      421 gtccgttgag tttcagtcat ttcc
//
LOCUS       DAWFIE010000177          436 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWFIE010000177 DAWFIE010000000
VERSION     DAWFIE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.178"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            27..>436
                     /locus_tag="WGQ71_002057"
     CDS             27..>436
                     /locus_tag="WGQ71_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0707989.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGTGKGNMPLRDAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGRHSRLAYA"
BASE COUNT          112 a          115 c          128 g           81 t
ORIGIN      
        1 caatcaatat tttttggaga tgatgaatgg gcaaaaccgt aaccttaacc gctggacaca
       61 gcaacaccga cccgggcgcg gtcaacggaa gcgaccgtga ggcggatttg gcgcaggata
      121 tgcgcaacat tgtggcttca atcctgcgta acgattacgg cttaaccgta cgcaccgacg
      181 gcacgggcaa aggcaatatg cccttgcgcg atgcggtcaa gctgattcgc ggctcggatg
      241 tggcgattga gtttcacacc aacgctgccg tcagcaaagc ggcgacaggc atcgaagcct
      301 tgagtaccgt taaaaacaaa cgctggtgtc aggtgttgag caaagccgtt gccaagaaaa
      361 ccggctggaa actgcgcggc gaagacggct ttaaacccga caatgcgggt cggcattcgc
      421 gcctggctta tgcgca
//
LOCUS       DAWFIE010000178          423 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWFIE010000178 DAWFIE010000000
VERSION     DAWFIE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.179"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT          120 a          113 c          109 g           81 t
ORIGIN      
        1 ccaacgctgc aagatttatc agaaacaact gaaaccgaac ggactggatt cccgcctgcg
       61 cgggaatgac gggattttag taaccgtagc aaccgcctgc gcgacggcta aggggcttca
      121 gcaaccgtag caactgcctg tgtgggaatg acggacaatg ggcttcagac ggcatctctt
      181 gcctgccgct aaaacagttt gccgcacaac tgttcaaacg cgtccgatat gtttcaacac
      241 acaggacgac acataaagca ccgccctatg tgtcgtcctg atttggaagg ggttacaccc
      301 ccctcccaaa taaagtctga tcctgccgcc ccgaaggaca gatgtccgag tggcggggtt
      361 tcaaccgaaa aggaaatacg atgaagtggt acaattagcg gcaatgcgga cagacaaatt
      421 aaa
//
LOCUS       DAWFIE010000179          423 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWFIE010000179 DAWFIE010000000
VERSION     DAWFIE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.180"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT          117 a           84 c          100 g          122 t
ORIGIN      
        1 ttcaatgcta aggcaattta tcgggaatga ctgaaactca aaaaactaga ttcccacttt
       61 cgtgggaatg acgtggtgca ggtttccgta tggatggatt cgtcattccc gcgcaggcgg
      121 gaatctagac attcaatgct aaggcaattt atcggaaatg actgaaactc aaaaagctgg
      181 attcccactt tcgtgggaat gacgcgatta gagtttcaaa atttattcta aatagctgaa
      241 actcaacgca ctggattccc gctttcgtgg gaatgacgaa ttttaggttt ctgattttgg
      301 ttttctgttt ttgtgaaaat aacaggattt tagcttgtgg gtatttaccg gaaaaaatag
      361 aaaccgctcc gccgtcattc ccgcgcaggc gggaatccag acctgtcggt gcggaaactt
      421 atc
//
LOCUS       DAWFIE010000180          422 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWFIE010000180 DAWFIE010000000
VERSION     DAWFIE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.181"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..>422
                     /locus_tag="WGQ71_002058"
     CDS             <1..>422
                     /locus_tag="WGQ71_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HEZ0707990.1"
                     /translation="YFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAVFGD
                     DYPTPDGTGMRDYIHVMDLAEGHIAAMQAKSNVAGTHLLNLGSGRASSVLEIIRAFEA
                     ASGLTIPYEVKPRRAGDLACFYADPSYTKAQIGWQTQR"
BASE COUNT           96 a          106 c          119 g          101 t
ORIGIN      
        1 tatttcaatc cgattggcgc gcacgaaagt ggtttgattg gcgagcagcc aaacggcatc
       61 ccgaataatt tgttgcctta tatctgccaa gtggcggcag gcaaactgcc gcaattggcg
      121 gtatttggcg atgactaccc tacccccgac ggcacgggga tgcgtgacta tattcatgtg
      181 atggatttgg cagaagggca tatcgcggct atgcaggcaa aaagtaatgt agcaggcacg
      241 catttgctga acttaggctc cggccgcgct tcttcggtgt tggaaatcat ccgcgcattt
      301 gaagcagctt cgggtttgac gattccgtat gaagtcaaac cgcgccgtgc cggtgatttg
      361 gcgtgcttct atgccgaccc ttcctataca aaggcgcaaa tcggctggca aacccagcgt
      421 ga
//
LOCUS       DAWFIE010000181          420 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWFIE010000181 DAWFIE010000000
VERSION     DAWFIE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.182"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            46..>420
                     /locus_tag="WGQ71_002059"
     CDS             46..>420
                     /locus_tag="WGQ71_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEZ0707991.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIDGPAAF
                     IETNIVGTYTLLEAARAYWHDMP"
BASE COUNT           99 a          125 c          108 g           88 t
ORIGIN      
        1 tcgtggcgtt gggtcagccg caaccccaac ggatatgggg attaaatgca aaccgcaaac
       61 aaaaaaacca tcctcgttac cggcggcgcg ggctttatcg gctctgccgt tgtccgtcat
      121 attatccgaa acacccaaga ttccgtcgtc aacctcgaca aactgaccta cgcgggcaat
      181 ctcgaatcgc tgaccgacat tgccgataat ccccgctacg cttttgagca agtggatatc
      241 tgcgaccgcg ccgaactcga ccgcgtattc gcgcaacacc ggccagatgc cgtgatgcac
      301 ttggcggcag aaagccatgt cgaccgcagt attgatgggc cggcggcatt tatcgaaacc
      361 aatattgtcg gcacttatac gcttttggaa gcggcgcgcg cttattggca cgatatgccg
//
LOCUS       DAWFIE010000182          393 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWFIE010000182 DAWFIE010000000
VERSION     DAWFIE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.183"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT           92 a           76 c          121 g          104 t
ORIGIN      
        1 gttgcgtagg actggattgt gaaaaggggc ggattcggtg aaaacggcgg aaatgtggga
       61 ttgatggaat cggtgggctg aagccctccc tacagagctt tcagacggca ttttttgcgt
      121 ttttcgggag ggggcggcaa atgaaacgcg tcattcccgc gcaggcggga atccggaccg
      181 gtcggtacgg gaacttatcg ggtaaaaagg tttctcaggt cctgagtcct ggattcccac
      241 tttcgtggga atgacgaatt ttaggtttct gattttggtt ttctgttttt gtgggaatga
      301 tgaaattttg agttttagga atttaccgga aaaaacagaa accgctccgc cgtcattccc
      361 gcgtaggcgg gaatccagac cttagaacaa cag
//
LOCUS       DAWFIE010000183          392 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWFIE010000183 DAWFIE010000000
VERSION     DAWFIE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.184"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT          103 a           99 c           80 g          110 t
ORIGIN      
        1 aaactccgtc attcttgcaa cttttcgtca ttcccgtgaa aacgggaatc tagaacttca
       61 aactttcaga taatctttga atattgccgt tgcccgacgg tttggattcc cgcttgcgcg
      121 ggaatgacgg tagagcggtt tcagttgctc ccgataaatg ccgcaatctc aaatcccgtc
      181 attccctcaa aaacagaaaa acaaaatcag aaacctaaaa tcccgtcatt cccgcagctc
      241 ttcgtcattc ccgtgaaagc gggaatctag accttttaaa ttttcacacg gcttttgaat
      301 attgccgtta cccgaaggtc tggattcccg cctgcacggg aatgacggca gagcggtttc
      361 agttgtttct aaataaattt ttgcagcgtt gg
//
LOCUS       DAWFIE010000184          390 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWFIE010000184 DAWFIE010000000
VERSION     DAWFIE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.185"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..>390)
                     /locus_tag="WGQ71_002060"
     CDS             complement(<1..>390)
                     /locus_tag="WGQ71_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0707992.1"
                     /translation="HTHFDGKAQVAEQIKGIGSITTATLMAMLPELGRLSHKQIASLA
                     GIAPHPRESGETKFKSRCFGGRSAVRKALYMATVAATRFKPLIRDFYQRLPSEGKPYK
                     VAVTACMRKLLTISNARMRDYFAENDTA"
BASE COUNT           78 a          115 c          102 g           95 t
ORIGIN      
        1 ggcggtatcg ttttcggcaa aataatcacg catccgggca ttcgatatcg tcagcagttt
       61 gcgcatacat gccgtaacgg caaccttata cggcttaccc tcggacggca ggcgttggta
      121 gaaatcccga ataagcggtt taaaacgtgt cgctgccacg gtagccatat acagcgcctt
      181 acgtaccgca gaccttccgc cgaagcagcg gcttttgaat ttggtttccc cgctctccct
      241 cgggtgcggg gcaatgccgg ccaaactcgc tatctgtttg tgcgacagcc gccccaattc
      301 gggcagcatc gccatcagcg tagccgtcgt tatcgaaccg atgcctttga tttgctctgc
      361 cacttgggct ttgccgtcaa aatgcgtgtg
//
LOCUS       DAWFIE010000185          373 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWFIE010000185 DAWFIE010000000
VERSION     DAWFIE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.186"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT           99 a           62 c          127 g           85 t
ORIGIN      
        1 gaatgtaggt tcgtgggaat gacgaagtgg aagttacccg aaacttaaaa caagcgaaac
       61 cgaacgaact agattcccac tttcgtggga atgacggaat gtaggttcgt gggaatgacg
      121 ggatgcaggt ttccgtatag atggattcgt cattcccgcg caggcgggaa tctagacatt
      181 caatgctaag gcaatttctc gggaatgact gaaactcaaa aaactggatt cccactttcg
      241 tgggaatgac gggatgcagg ttcgtgggaa tgacgggatg caggttcgtg gggatgacgg
      301 gatgcaggtt cgtggggatg acgggatgca ggttcgtggg aatgacggaa tgtaggttcg
      361 tgggaatgac gtg
//
LOCUS       DAWFIE010000186          369 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWFIE010000186 DAWFIE010000000
VERSION     DAWFIE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.187"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(140..>369)
                     /locus_tag="WGQ71_002061"
     CDS             complement(140..>369)
                     /locus_tag="WGQ71_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ0707993.1"
                     /translation="DGWYDFRCQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAKS
                     EQRLSVLSEWVGQLETEYSELNLNRYSVASP"
BASE COUNT           89 a           87 c          102 g           91 t
ORIGIN      
        1 ggtatttggg gcgaaaacag ccgaaaacct gtgttggggt ttcggctgtc gggagggaaa
       61 ggaattttgc aaaggtctca aatagtacgg aaccgattca cttggtgctt cagcacctta
      121 gagaatcgtt ctctttgagc taaggcgagg caacgctgta ccggtttaaa tttaattcac
      181 tatattcggt ttccaactga ccgacccatt ctgataagac cgacaggcgt tgttcggatt
      241 tcgccacgaa cgggttttcg gtatcccacg cgtagcctgc caaaatcgaa gaaagcatac
      301 ggctgatttc cgggctgcgg tcgggcgcgt acacgacgtt ttggcagcgg aaatcgtacc
      361 agccgtcca
//
LOCUS       DAWFIE010000187          365 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWFIE010000187 DAWFIE010000000
VERSION     DAWFIE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.188"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT          106 a           86 c          100 g           73 t
ORIGIN      
        1 gtgggaatga cgattcagaa gttgcctgaa acctaaaaaa ctgaaaccga acgagccgga
       61 tttccgcttt cgcgggaatg acggcgcata agttcccgtg cggacagacc tagattccca
      121 cctgtgtggg aatgacgatg cagaagttgc ccgaaaccaa aaaactgaag ccgaacggtc
      181 tggattcccg ctttcgcggg aatgacggcg cataagttcc cgtgcggaca gacctagatt
      241 cccacctgcg tgggaatgac gattcagaag ttgcccgaaa ctcaaaaaaa acgaaaccga
      301 acgaaccgga ttcccgcttt tacgggaatg acgggatttt gggttgtggt aatttatcgg
      361 gaaaa
//
LOCUS       DAWFIE010000188          361 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWFIE010000188 DAWFIE010000000
VERSION     DAWFIE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.189"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..152)
                     /locus_tag="WGQ71_002062"
     CDS             complement(<1..152)
                     /locus_tag="WGQ71_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0707994.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEI"
BASE COUNT           95 a           83 c           85 g           98 t
ORIGIN      
        1 aaaatttcaa acggataccg ttcgcgcacc gccattaccg cctgccgcaa agccaaacgc
       61 accgcatcat cggtcaaaat cttctgccgt ttattggtta caaccgtaaa aaagtaagtg
      121 ccgccattgc ggtaaaaacg acggtatttc atagtattat gctcggaatg attttgtagg
      181 tcggattctt gaattcgaca ttttgggcat tgctgcaatg gattgcaatg atgggaatgt
      241 taaaggtttt gtcggataca agtatccgac ctacgcttgc tgaaccgtca ttcccacgga
      301 agtgggaatc tagaatctca gggtttcagt catttccgat agattcccgc cgcgtcaggg
      361 g
//
LOCUS       DAWFIE010000189          349 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWFIE010000189 DAWFIE010000000
VERSION     DAWFIE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.190"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT          118 a           86 c           57 g           88 t
ORIGIN      
        1 cagatattcg aatatcgaat ttgccttatg attacggttt tctgtcttat ctgccttaaa
       61 acgccgatat tccgtttgaa ccaaattata tttgccgaaa cgctcaaaaa tctcccttac
      121 ctgcccgaca gacatcaagc cttcgttgtt gtagctgaga aaaatatatt gaaagtccgc
      181 atccctaatc aaagccccaa ataccggaac cacatccgat tttgagcaag tccgcgctaa
      241 aacacatcgt acagcgcaaa accggatggg aatggcaaaa tccggaaaca aacatttttc
      301 aaagcataca aacaaaaaaa taccaaccgg aggttggtat tccttaact
//
LOCUS       DAWFIE010000190          342 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWFIE010000190 DAWFIE010000000
VERSION     DAWFIE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.191"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..291
                     /locus_tag="WGQ71_002063"
     CDS             <1..291
                     /locus_tag="WGQ71_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0707995.1"
                     /translation="DNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEYTIRDEADFA
                     WHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSIGYD"
BASE COUNT           90 a           69 c           86 g           97 t
ORIGIN      
        1 gacaatgatt ctgcttattc ggaacgctgg cggcaaatca agcggcacag ccaatattta
       61 atcggcggca atctcaggct ttggcaaaaa cgcttttggg aatatactat ccgcgatgag
      121 gccgattttg cctggcattt tgattatctg catttcaatc cggtcaaaca tggctatgta
      181 ggacaaattt ccgattgggg gttttctacg tttcaccgtt atgtcaaaca gggtatttat
      241 ccgcataatt ggggtggcgg caatgcggac ttttctattg gatacgattg aagtaatgtc
      301 ggattcgaga atccgaccta cggaaaactg aaagagcatc gg
//
LOCUS       DAWFIE010000191          323 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWFIE010000191 DAWFIE010000000
VERSION     DAWFIE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.192"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            56..241
                     /locus_tag="WGQ71_002064"
     CDS             56..241
                     /locus_tag="WGQ71_002064"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0707996.1"
                     /translation="MDGFVVPAKAGIRNRKPQKFIGKTETQKQRKPNGQDSRLRGNDG
                     SYITQKIETKQMGFPLA"
BASE COUNT          100 a           69 c           84 g           70 t
ORIGIN      
        1 cttgtgggaa tgacgggatg taggttcgta ggaatgacgt ggtgcaggtt tccgtatgga
       61 tggattcgtc gttcccgcga aagcgggaat ccggaaccgg aaaccacaaa aatttatcgg
      121 gaaaaccgaa actcaaaaac aacggaaacc gaacggacag gattcccgcc tgcgcgggaa
      181 tgacggctca tacatcaccc aaaaaattga aaccaaacag atgggattcc ctcttgcgtg
      241 aggctgacag atgccgtctg aaagactttc agacggcata gctttttctc tttgaattta
      301 tagtggatta acaaaaatca gga
//
LOCUS       DAWFIE010000192          320 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWFIE010000192 DAWFIE010000000
VERSION     DAWFIE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.193"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT           96 a           89 c           56 g           79 t
ORIGIN      
        1 tttttttgaa tttcgggcaa cgcttaaatc ttcattccgc gcaggcggaa attatcggtg
       61 cggtacggca acttttttcg atatgaaaag accgtcattc ctgtaaaaac aaaaaatcaa
      121 aaacagaaaa ttgaaattcg tcattcccgc gcaggcggga atccaggacg taaaatctga
      181 gacctttgca aaattccttt ccctcccgac agccgaaacc caaacacagg ttttcagctg
      241 ttttcgcccc aaataccgcc taattttacc caaatacccc ctgaatcctc cccggatacc
      301 cgataatcag gcatccgagc
//
LOCUS       DAWFIE010000193          314 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWFIE010000193 DAWFIE010000000
VERSION     DAWFIE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.194"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(158..>314)
                     /locus_tag="WGQ71_002065"
     CDS             complement(158..>314)
                     /locus_tag="WGQ71_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0707997.1"
                     /translation="RRLGLGVIAGVGFDITSNLTLDAGYRYHNWGRLENTRFKTHEAS
                     LGVRYRF"
BASE COUNT           74 a           72 c           98 g           70 t
ORIGIN      
        1 atttcgagat tgcggtgttg tcgggaatga caaatttcaa gattacggtg ttgtcgggaa
       61 tgacggttcg ggtatttcca cgcccgcccc gcgtttgtca acggcaggtg aatcaaaaat
      121 gccgtctgaa ggttcagacg gcatcggtat cggggaatca gaagcggtag cgcacgccca
      181 atgaggcttc gtgggttttg aagcgggtgt tttccaagcg tccccagttg tggtaacggt
      241 agccagcatc taaagtcaga ttggacgtga tgtcgaaacc gacaccggcg atgacaccaa
      301 gacccaagcg gcgg
//
LOCUS       DAWFIE010000194          313 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWFIE010000194 DAWFIE010000000
VERSION     DAWFIE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.195"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..>313
                     /locus_tag="WGQ71_002066"
     CDS             <1..>313
                     /locus_tag="WGQ71_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0707998.1"
                     /translation="QLTEKGLKIEKASAAVIDATIIQTAGSKQRQAIEVDEEGQVSGQ
                     TTPSKDKDARWTKKNGLYRLGYKQHTRTDEEGYIKKLHITPANAHECKHLLPLLEGLP
                     KG"
BASE COUNT          108 a           82 c           72 g           51 t
ORIGIN      
        1 gccaactgac cgaaaaaggt ttaaaaatag agaaagcatc cgccgccgtc attgatgcca
       61 ccattattca gactgccggc agcaaacagc gccaggccat agaagtcgac gaggaaggac
      121 aagtcagcgg ccaaaccaca ccgagtaaag ataaagatgc ccgttggaca aagaaaaacg
      181 gtttatacag actcggttac aaacaacata cccgtaccga tgaggaaggc tatatcaaga
      241 aactgcacat cacccccgcc aatgcccatg agtgcaaaca cctgttgcct ttgttggaag
      301 ggttacccaa agg
//
LOCUS       DAWFIE010000195          310 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWFIE010000195 DAWFIE010000000
VERSION     DAWFIE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.196"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
BASE COUNT           97 a           83 c           55 g           75 t
ORIGIN      
        1 ccatccgcac ggaaacctat atcccgtcat tcttacgaac ctacatcccg tcattccctc
       61 aaaaacagaa aaccaaaatt agaaacctaa aatcccgtca ttcccacgaa agtgggaatc
      121 tagttcgttg agtttcagtt atttagaata aattttgaaa ctttaatccc gtcattcccg
      181 caaaagcggg aatccagaac gtaaaatcta aagaaaccgt tttacccgat aagtttccgc
      241 accgacagac ctagattccc gcctgcgcgg gaatgacggc tgcagatgcc cgacggtctt
      301 tatagtggat
//
LOCUS       DAWFIE010000196          268 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWFIE010000196 DAWFIE010000000
VERSION     DAWFIE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.denovo.197"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            <1..>268
                     /locus_tag="WGQ71_002067"
     CDS             <1..>268
                     /locus_tag="WGQ71_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HEZ0707999.1"
                     /translation="RSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAAKAYDQID
                     NAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGA"
BASE COUNT           83 a           82 c           57 g           46 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggcggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgcccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgcc
//
LOCUS       DAWFIE010000197         5096 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S186
            SAMN37720371-rid20220313.guided.104, whole genome shotgun sequence.
ACCESSION   DAWFIE010000197 DAWFIE010000000
VERSION     DAWFIE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720371
            Sequence Read Archive: SRR26318893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985367.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985367.1
            Genome Coverage       :: 245x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 20:59:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,071
            CDSs (total)                      :: 2,015
            Genes (coding)                    :: 1,901
            CDSs (with protein)               :: 1,901
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 43 of 114
            Pseudo Genes (incomplete)         :: 68 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720371-rid20220313.guided.104"
                     /strain="S186"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2021"
                     /collected_by="Suzhou CDC"
     gene            complement(296..1810)
                     /gene="katA"
                     /locus_tag="WGQ71_002068"
     CDS             complement(296..1810)
                     /gene="katA"
                     /locus_tag="WGQ71_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ0708000.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITQYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPASYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            complement(2073..2690)
                     /gene="tetR(B)"
                     /locus_tag="WGQ71_002069"
     CDS             complement(2073..2690)
                     /gene="tetR(B)"
                     /locus_tag="WGQ71_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HEZ0708001.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLNGFM"
     gene            2772..3977
                     /gene="tet(B)"
                     /locus_tag="WGQ71_002070"
     CDS             2772..3977
                     /gene="tet(B)"
                     /locus_tag="WGQ71_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HEZ0708002.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIVGPIIGGFAGEISPHSP
                     FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            4074..4925
                     /locus_tag="WGQ71_002071"
     CDS             4074..4925
                     /locus_tag="WGQ71_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ0708003.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVEDWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERLGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESLEIPAA
                     HKRKTAE"
BASE COUNT         1180 a         1195 c         1311 g         1410 t
ORIGIN      
        1 agattttacg ttctggattc ccactttcgt gggaatgacg cggaaaagtt ttcgtgccgg
       61 tttgaaccgg tggggatggt gcgggaatga cggcggaaaa gtgggctgtg gggcgggaat
      121 gacgggatat tttgcgttta ataaaaaacg cccactgaaa cggcgggggt ggggtgtgga
      181 aatgccgtct gaagcggtca aacgacgttt cagacggcat ttttatgccc ggttatttcc
      241 ggtagcggac ggcgcgggac aggatttctt caatttccat ccacataatg cccccttaca
      301 gcaaaccggg ctggccgagt gcggggtcgg tcgcgcgggc ggcttgtgcg tcttcgacag
      361 tcagtccgag ggcttttgcc acgccttcac cgtatgccgg atcgcaacgg tagcagttgc
      421 ggatatggcg gtatttgatg aagtcgggcg cgtcgcccat tgcggcggcg gtgttgccga
      481 acaatgcctg tttctgcgcg tcgttcatca ggttgaacag ggcgcgcggt tggctgaagt
      541 agtcgtcatc atcttggcgg tagtcccagt gtgccgcgtc gccgttgatt ttcaaaggcg
      601 gttcggcgaa gtcgggctgc tgctgccatt gtccgaagct gttgggttcg tagtgcggca
      661 ggctgccgta gttgccgtcg gctctgcctt gtccgtcgcg ctggttgctg tgaacagggc
      721 aacgcggacg gttgacaggg atttggcgga agttcacgcc caagcggtag cgttgcgcgt
      781 cggcgtaatt gaacaaacgc gcctgcaaca ttttatctgg gctggcgccg acaccgggaa
      841 cgaggttgct cggtgcgaag gcggattgtt ccacatcggc gaagaagttt tcgggattgc
      901 ggttcaactc gaattcgccc acttcaatca gcggatagtc ttttttcggc caaactttgg
      961 tcaagtcaaa cggatgataa ggtacttttt ccgcgtctgc ttcaggcatg acttggatgt
     1021 acatcgtcca tttcggaaac tcgccgcgtt cgatggcttc gtacaggtcg cgctgatggc
     1081 tttcgcggtc gtcggcgatg atttttgcgg cttcttcgtt ggtcaggttt ttaatgcctt
     1141 gttgggtgcg gaaatgaaat ttcacccaga aacgctcgcc tgcttcgttc cagaagctgt
     1201 aagtatgcga accgaagccg tgcatatggc ggtagctggc gggaataccg cggtcgctca
     1261 tgacgatggt aacttggtgc aacgcttcag gcagcagcgt ccagaagtcc cagttgtttg
     1321 tggcggaacg catattggtg cgcggatcgc gtttgacggc tttgttcagg tcagggaatt
     1381 tacgcgggtc gcgcaggaag aacacgggcg tgttgttgcc gaccacatcc cagttgcctt
     1441 cttcggtata aaatttcaat gcaaagcctc ggatgtcgcg ttccgcatcg gctgcgccgc
     1501 gttcgcctgc cacggtggtg aaacgggcga acatctcggt ttttttgccg acttcgctga
     1561 agattttggc gcgggtgtat tgggtgatgt cgtgcgttac ggtaaacgtg ccgaacgcgc
     1621 ccgaaccttt ggcgtgcata cggcgttcgg ggatgacttc gcgcacgaag tcggcgagtt
     1681 tttcattcag ccacaaatcc tgcgccagca gggggccgcg cggaccggcg gtcaggctgt
     1741 tttgattgtc ggcaacaggc gcgccgttgt tcatggtcag atgggttacg gggcatttgg
     1801 aggtagtcat agctcgtgtt ccttttcctt ggttaatcaa aagatacgac aaacggctta
     1861 cagtacgatt tggcggaaag cgtattcgta accggtttct tgattgcaat aaatttcttg
     1921 aatcgacatt ttgttttcct tttgtaaaaa ctatggatga gactatacgc taagattttt
     1981 gctattaaaa ctatgaaatc gatttaatat tgttataagc aatcggtttt tgattttcgt
     2041 ttgttttttg ttatcgaaac gaatcaaaat ccctacataa aaccattaag ttgtttttct
     2101 aatccgcata tgatcaattc aaggccgaat aagaaggctg gctctgcacc ttggtgatca
     2161 aataattcga tagcttgtcg taataatggc ggcatactat cagtagtagg tgtttccctt
     2221 tcttctttag cgacttgatg ctcttgatct tccaatacgc aacctaaagt aaaatgcccc
     2281 acagcgctga gtgcatataa tgcattctct agtgaaaaac cttgttggca taaaaaggct
     2341 aattgatttt cgagagtttc atactgtttt tctgtaggcc gtgtacctaa atgtactttt
     2401 gctccatcgc gatgacttag taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa
     2461 tcttgccagc tttccccttc taaagggcaa aagtgagtat ggtgcctatc taacatctca
     2521 atggctaagg cgtcgagcaa agcccgctta ttttttacat gccaatacaa tgtaggctgc
     2581 tctacaccta gcttctgggc gagtttacgg gttgttaaac cttcgattcc gacctcatta
     2641 agcagctcta atgcgctgtt aatcacttta cttttatcta atctagacat cattaattcc
     2701 taatttttgt tgacactcta tcattgatag agttattcta ccactcccta tcagtgatgg
     2761 agaaaagtga aatgaatagt tcgacaaaga tcgcattggt aattacgtta ctcgatgcca
     2821 tggggattgg ccttatcatg ccagtcttgc caacgttatt acgtgaattt attgcttcgg
     2881 aagatatcgc taaccacttt ggcgtattgc ttgcacttta tgcgttaatg caggttatct
     2941 ttgctccttg gcttggaaaa atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt
     3001 cattaatagg cgcatcgctg gattacttat tgctggcttt ttcaagtgcg ctttggatgc
     3061 tgtatttagg ccgtttgctt tcagggatca caggagctac tggggctgtc gcggcatcgg
     3121 tcattgccga taccacctca gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa
     3181 gttttgggct tggtttaata gtggggccta ttattggtgg ttttgcagga gagatttcac
     3241 cgcatagtcc cttttttatc gctgcgttgc taaatattgt cactttcctt gtggttatgt
     3301 tttggttccg tgaaaccaaa aatacacgtg ataatacaga taccgaagta ggggttgaga
     3361 cgcaatcgaa ttcggtatac atcactttat ttaaaacgat gcccattttg ttgattattt
     3421 atttttcagc gcaattgata ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa
     3481 atcgttttgg atggaatagc atgatggttg gcttttcatt agcgggtctt ggtcttttac
     3541 actcagtatt ccaagccttt gtggcaggaa gaatagccac taaatggggc gaaaaaacgg
     3601 cagtactgct cggatttatt gcagatagta gtgcatttgc ctttttagcg tttatatctg
     3661 aaggttggtt agttttccct gttttaattt tattggctgg tggtgggatc gctttacctg
     3721 cattacaggg agtgatgtct atccaaacaa agagtcatca gcaaggtgct ttacagggat
     3781 tattggtgag ccttaccaat gcaaccggtg ttattggccc attactgttt gctgttattt
     3841 ataatcattc actaccaatt tgggatggct ggatttggat tattggttta gcgttttact
     3901 gtattattat cctgctatcg atgaccttca tgttaacccc tcaagctcag gggagtaaac
     3961 aggagacaag tgcttagtta tttcgtcacc aaatgatgtt attccgctta taatgatgtt
     4021 tcccggcatc atctgccgcc gacaggccaa aagaataatt ctctcgcatt atcatgacgt
     4081 tactcggaat aaagctcaaa cagacccagc agctcaacca gcggctgcaa caatctttgc
     4141 gcgtattgca gatgtcgggt atcgaacttg aacgcgaggt cgaagactgg ctgtcggaca
     4201 accccctgct cgaacgcaaa gacacggatg aattttccga tgccgagttc agccattaca
     4261 ccgcgcctgc ccgtcaaatc ggcggagacg aaggcgaaga tatgctgtcc aacatcgccg
     4321 gcgagcagga tttcaagcaa tacctgcacg cgcaagtgtg cgaacacccg ctttccgacc
     4381 aagaagccgc ctgtgtccac atccttatcg atttccttga cgagcagggc tacctgaccg
     4441 acagcatcga agacatcctc gaccatacgc ccttggagtg gatgttggat gaagcaatgc
     4501 tgaaacaagc cctgaccgca ttgaaaaaat tcgacccggc aggcgtggcc gccgccgatt
     4561 tgaacgaatc gctgatactg cagatagaaa gattgggcga atgtgctgcc aaacccgccg
     4621 ccctgcatat cgtccgaaac gccctcgaca gcattgacgg caaccgcagc caaaccctcg
     4681 cacgaataaa aaaacacctg ccccaaaccg acagcggcac actcgaagcc gcactcgacc
     4741 tcattgcttc gctcaatccc tttcccgccg ccggttttgc ctcgtccaca cccacgccgt
     4801 attctgacga ggcactcgcc aacctgctgg ctttccgcgg catggaggtt tctcgccgca
     4861 ccattgccaa atacagagaa tcccttgaga ttccggcagc acacaaacgc aaaaccgcag
     4921 aataattgct gaataatctt ataaagacaa caaaccaaaa gccggcattt ctgcgaaagc
     4981 gggaatgccg aatccgtccg cgcggaaacc tgcatcccgt cattcccgcg aaagagggaa
     5041 tctagaaacg caaagctgca agaatttatc ggaaatgacc gaaactcaac gaacct
//
